Prkd3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkd3
Entrez Gene ID	75292
Full Name	protein kinase D3
Synonyms	4930557O20Rik,5730497N19Rik,PKD3,Pkcnu,Prkcn
General protein information	Preferred Names protein kinase D3 Names serine/threonine-protein kinase D3 nPKC-nu protein kinase C nu type protein kinase C, nu
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_029239.3	NP_083515.2	serine/threonine-protein kinase D3 isoform 2
XM_030250123.1	XP_030105983.1	serine/threonine-protein kinase D3 isoform X1
NM_001171004.1	NP_001164475.1	serine/threonine-protein kinase D3 isoform 1
NM_001171005.1	NP_001164476.1	serine/threonine-protein kinase D3 isoform 3

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000070	XP_311085.5
Rattus norvegicus (Norway rat)	Prkd3	NP_001019434.2
Homo sapiens (human)	PRKD3	NP_005804.1
Pan troglodytes (chimpanzee)	PRKD3	XP_001166998.1
Canis lupus familiaris (dog)	PRKD3	XP_005630396.1
Caenorhabditis elegans (roundworm)	dkf-2	NP_507239.2
Danio rerio (zebrafish)	prkd3	XP_685483.4
Caenorhabditis elegans (roundworm)	dkf-2	NP_001256726.1
Macaca mulatta (Rhesus monkey)	PRKD3	XP_001108366.1
Bos taurus (cattle)	PRKD3	NP_001192467.1
Mus musculus (house mouse)	Prkd3	NP_001164475.1
Gallus gallus (chicken)	PRKD3	XP_419526.2
Drosophila melanogaster (fruit fly)	PKD	NP_001262705.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein kinase D regulates positive selection of CD4
Ishikawa E, Kosako H, Yasuda T, Ohmuraya M, Araki K, Kurosaki T, Saito T, Yamasaki S
Nature communications712756(2016 Sep)

PKD3 deficiency causes alterations in microtubule dynamics during the cell cycle.
Zhang T, Braun U, Leitges M
Cell cycle (Georgetown, Tex.)15(14)1844-54(2016 07)

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Ray JP, Staron MM, Shyer JA, Ho PC, Marshall HD, Gray SM, Laidlaw BJ, Araki K, Ahmed R, Kaech SM, Craft J
Immunity43(4)690-702(2015 Oct)

Protein kinase D-HDAC5 signaling regulates erythropoiesis and contributes to erythropoietin cross-talk with GATA1.
Delehanty LL, Bullock GC, Goldfarb AN
Blood120(20)4219-28(2012 Nov)

Inducible silencing of protein kinase D3 inhibits secretion of tumor-promoting factors in prostate cancer.
LaValle CR, Zhang L, Xu S, Eiseman JL, Wang QJ
Molecular cancer therapeutics11(7)1389-99(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prkd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu87281	XM_030250123.1 Latest version!	Mus musculus protein kinase D3 (Prkd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu06670	NM_029239.3 Latest version!	Mus musculus protein kinase D3 (Prkd3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu11786	NM_001171004.1 Latest version!	Mus musculus protein kinase D3 (Prkd3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu11790	NM_001171005.1 Latest version!	Mus musculus protein kinase D3 (Prkd3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkd3 ( XM_030250123.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105983.1 Mus musculus protein kinase D3 (Prkd3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu87281
Clone ID Related Accession (Same CDS sequence)	XM_030250123.1
Accession Version	XM_030250123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase D3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGTGG TGCAGTCCAT CAAGCACACA AAGAGGAGGA GCAGCACAGT TGTGAAGGAA 
GGGTGGATGG TCCACTACAC CAGCAGGGAC AACCTGAGAA AGAGACATTA TTGGAGACTT 
GACAGTAAAT GTCTAACATT GTTTCAAAAT GAATCTGGAT CAAAGTATTA TAAGGAAATT 
CCACTTTCAG AAATTCTCCG TGTATCTTCA CCGCAAGATT TCACCAGCAT TTCACAAGGC 
AGTAACCCAC ACTGTTTTGA AATCATCACC GATACCGTGG TATACTTTGT TGGTGAGAAC 
AATGGGAGCA GCTCTCATAA TCCTGTTCTC GCTGCCACTG GAGTGGGACT AGATGTGGCG 
CAGAGCTGGG AAAAAGCAAT TCGCCAGGCC CTCATGCCTG TCACTCCTCA AGCAAGTGTT 
TGCACTTCTC CGGGGCAAGG GAAGGACCAC AAAGATCTGG CTACCAGTAT CTCCGTGTCT 
AACTGTCAGG TTCAGGAGAA TGTCGACATC AGCAGCGTTT ACCAGATCTT TGCGGACGAG 
GTGCTTGGTT CTGGCCAGTT TGGCATTGTT TATGGAGGAA AACACAGAAA GACGGGAAGG 
GATGTGGCTA TTAAAGTGAT TGATAAAATG AGGTTCCCCA CAAAGCAAGA GAGTCAGCTC 
CGGAACGAAG TGGCCATTTT ACAGAATCTG CACCATCCTG GGATTGTAAA CCTGGAGTGT 
ATGTTTGAAA CCCCAGAACG AGTCTTCGTG GTGATGGAAA AGCTCCATGG AGATATGTTG 
GAAATGATTC TGTCCAGTGA AAAGAGTCGA CTTCCAGAAC GGATTACCAA ATTCATGGTC 
ACACAGATAC TTGTTGCCTT GAGGAATTTA CATTTCAAGA ATATTGTGCA CTGTGATTTA 
AAGCCCGAAA ATGTGCTGCT TGCATCCGCA GAGCCATTTC CTCAGGTGAA GCTGTGTGAC 
TTTGGGTTTG CCCGCATCAT TGGTGAGAAG TCGTTCCGGA GGTCAGTGGT AGGAACTCCA 
GCCTACTTAG CCCCTGAGGT TCTCAGGAGC AAAGGCTACA ACCGTTCTCT AGACATGTGG 
TCGGTGGGCG TCATCGTTTA TGTGAGCCTC AGTGGCACAT TCCCATTCAA TGAAGATGAA 
GATATAAATG ACCAAATCCA AAACGCTGCG TTTATGTACC CACCAAATCC ATGGCGAGAA 
ATTTCCAGTG AAGCAATTGA CTTGATAAAC AACTTGCTTC AAGTGAAGAT GAGAAAACGA 
TACAGTGTTG ACAAATCTCT CAGTCATCCT TGGCTACAGG ATTATCAGAC TTGGCTTGAC 
CTCAGAGAAT TTGAAACTCG CATTGGAGAA CGTTACATTA CACACGAAAG TGATGATGCT 
CGCTGGGAAA TACACGCATA CACGCACAAT CTGGAATACC CAAAGCATTT CATTATGGCC 
CCCAACCCAG ACGACATGGA GGAGGATCCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105983.1
CDS	648..2120
Translation	MRVVQSIKHTKRRSSTVVKEGWMVHYTSRDNLRKRHYWRLDSKCLTLFQNESGSKYYKEIPLSEILRVSSPQDFTSISQGSNPHCFEIITDTVVYFVGENNGSSSHNPVLAATGVGLDVAQSWEKAIRQALMPVTPQASVCTSPGQGKDHKDLATSISVSNCQVQENVDISSVYQIFADEVLGSGQFGIVYGGKHRKTGRDVAIKVIDKMRFPTKQESQLRNEVAILQNLHHPGIVNLECMFETPERVFVVMEKLHGDMLEMILSSEKSRLPERITKFMVTQILVALRNLHFKNIVHCDLKPENVLLASAEPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRSKGYNRSLDMWSVGVIVYVSLSGTFPFNEDEDINDQIQNAAFMYPPNPWREISSEAIDLINNLLQVKMRKRYSVDKSLSHPWLQDYQTWLDLREFETRIGERYITHESDDARWEIHAYTHNLEYPKHFIMAPNPDDMEEDP

Target ORF information:

RefSeq Version	XM_030250123.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase D3 (Prkd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250123.1

ORF Insert Sequence:

ATGAGGGTGG TGCAGTCCAT CAAGCACACA AAGAGGAGGA GCAGCACAGT TGTGAAGGAA 
GGGTGGATGG TCCACTACAC CAGCAGGGAC AACCTGAGAA AGAGACATTA TTGGAGACTT 
GACAGTAAAT GTCTAACATT GTTTCAAAAT GAATCTGGAT CAAAGTATTA TAAGGAAATT 
CCACTTTCAG AAATTCTCCG TGTATCTTCA CCGCAAGATT TCACCAGCAT TTCACAAGGC 
AGTAACCCAC ACTGTTTTGA AATCATCACC GATACCGTGG TATACTTTGT TGGTGAGAAC 
AATGGGAGCA GCTCTCATAA TCCTGTTCTC GCTGCCACTG GAGTGGGACT AGATGTGGCG 
CAGAGCTGGG AAAAAGCAAT TCGCCAGGCC CTCATGCCTG TCACTCCTCA AGCAAGTGTT 
TGCACTTCTC CGGGGCAAGG GAAGGACCAC AAAGATCTGG CTACCAGTAT CTCCGTGTCT 
AACTGTCAGG TTCAGGAGAA TGTCGACATC AGCAGCGTTT ACCAGATCTT TGCGGACGAG 
GTGCTTGGTT CTGGCCAGTT TGGCATTGTT TATGGAGGAA AACACAGAAA GACGGGAAGG 
GATGTGGCTA TTAAAGTGAT TGATAAAATG AGGTTCCCCA CAAAGCAAGA GAGTCAGCTC 
CGGAACGAAG TGGCCATTTT ACAGAATCTG CACCATCCTG GGATTGTAAA CCTGGAGTGT 
ATGTTTGAAA CCCCAGAACG AGTCTTCGTG GTGATGGAAA AGCTCCATGG AGATATGTTG 
GAAATGATTC TGTCCAGTGA AAAGAGTCGA CTTCCAGAAC GGATTACCAA ATTCATGGTC 
ACACAGATAC TTGTTGCCTT GAGGAATTTA CATTTCAAGA ATATTGTGCA CTGTGATTTA 
AAGCCCGAAA ATGTGCTGCT TGCATCCGCA GAGCCATTTC CTCAGGTGAA GCTGTGTGAC 
TTTGGGTTTG CCCGCATCAT TGGTGAGAAG TCGTTCCGGA GGTCAGTGGT AGGAACTCCA 
GCCTACTTAG CCCCTGAGGT TCTCAGGAGC AAAGGCTACA ACCGTTCTCT AGACATGTGG 
TCGGTGGGCG TCATCGTTTA TGTGAGCCTC AGTGGCACAT TCCCATTCAA TGAAGATGAA 
GATATAAATG ACCAAATCCA AAACGCTGCG TTTATGTACC CACCAAATCC ATGGCGAGAA 
ATTTCCAGTG AAGCAATTGA CTTGATAAAC AACTTGCTTC AAGTGAAGAT GAGAAAACGA 
TACAGTGTTG ACAAATCTCT CAGTCATCCT TGGCTACAGG ATTATCAGAC TTGGCTTGAC 
CTCAGAGAAT TTGAAACTCG CATTGGAGAA CGTTACATTA CACACGAAAG TGATGATGCT 
CGCTGGGAAA TACACGCATA CACGCACAAT CTGGAATACC CAAAGCATTT CATTATGGCC 
CCCAACCCAG ACGACATGGA GGAGGATCCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkd3 ( NM_029239.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083515.2 Mus musculus protein kinase D3 (Prkd3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu06670
Clone ID Related Accession (Same CDS sequence)	NM_029239.3
Accession Version	NM_029239.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571587200000
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase D3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC151293.2 and AC154274.1. On Jan 16, 2010 this sequence version replaced NM_029239.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC037012.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCAA ATAATTCCCC TCCATCAGCC CAGAAATCTG TGTTCCCTGC AACTGTTTCT 
GCTGTGCTTC CAGCTCCTTC TCCGTGTTCA AGTCCCAAAA CAGGACTCTC AGCTCGGCTC 
TCTAATGGCA GCTTCAGTGC CCCGTCACTC ACAAACTCTA GAGGCTCAGT GCACACAGTT 
TCATTTCTAC TTCAGATTGG CCTCACACGA GAGAGTGTTA CCATCGAGGC CCAGGAACTG 
TCTCTCTCTG CTGTCAAAGA CCTCGTGTGC TCCATTGTTT ATCAGAAGTT TCCAGAGTGT 
GGATTCTTTG GCATGTATGA TAAAATTCTT CTCTTTCGCC ACGACATGAA CTCAGAAAAC 
ATTTTGCAGC TGATTACCTC AGCAGATGAA ATACATGAAG GGGACCTGGT AGAAGTTGTC 
CTGTCAGCTT TGGCCACAGT GGAAGACTTC CAGATCCGCC CTCACGCTCT CTATGTACAT 
TCTTACAAAG CACCTACTTT TTGTGATTAC TGTGGTGAAA TGCTCTGGGG ACTGGTACGT 
CAAGGATTAA AATGTGAAGG CTGTGGATTA AATTATCATA AACGATGTGC CTTCAAGATT 
CCAAATAACT GCAGTGGAGT AAGAAAGAGA CGTCTGTCAA ATGTATCTCT GCCAGGACCT 
GGCCTCTCAG TTCCAAGACC CCTGCAGCCT GAGTGTGTGC CCCTTCTCAG TGAAGAGTCA 
CATACTCACC AAGAGCCCAG TAAGAGAATC CCGTCGTGGA GTGGTCGTCC GATCTGGATG 
GAAAAGATGG TAATGTGCAG GGTCAAAGTC CCTCACACAT TTGCTGTCCA CTCTTATGGC 
CGCCCGACCA TATGCCAGTA CTGCAAGCGC CTGCTGAAAG GCCTCTTCCG CCAAGGGATG 
CAGTGTAAAG ATTGCAAATT CAACTGCCAT AAACGCTGTG CATCCAAAGT ACCAAGAGAC 
TGCCTTGGCG AAGTTACTTT CAATGGAGAG CCGTGCAGCG TGGGGACTGA TGCAGACATG 
CCCATGGATA TTGACAGTAG TGATGTGAAC AGTGATGGCA GTAGGGGCTT GGATGACTCA 
GAGGAGCCGT CCCCGCCCGA AGACAAGATG TTCTTCCTCG ACCCGACTGA TCTCGACGTG 
GAGCGAGATG AGGAGACTGT TAAGACAATC AGTCCATCCA CCAGCAATAA TATCCCGCTG 
ATGAGGGTGG TGCAGTCCAT CAAGCACACA AAGAGGAGGA GCAGCACAGT TGTGAAGGAA 
GGGTGGATGG TCCACTACAC CAGCAGGGAC AACCTGAGAA AGAGACATTA TTGGAGACTT 
GACAGTAAAT GTCTAACATT GTTTCAAAAT GAATCTGGAT CAAAGTATTA TAAGGAAATT 
CCACTTTCAG AAATTCTCCG TGTATCTTCA CCGCAAGATT TCACCAGCAT TTCACAAGGC 
AGTAACCCAC ACTGTTTTGA AATCATCACC GATACCGTGG TATACTTTGT TGGTGAGAAC 
AATGGGAGCA GCTCTCATAA TCCTGTTCTC GCTGCCACTG GAGTGGGACT AGATGTGGCG 
CAGAGCTGGG AAAAAGCAAT TCGCCAGGCC CTCATGCCTG TCACTCCTCA AGCAAGTGTT 
TGCACTTCTC CGGGGCAAGG GAAGGACCAC AATCTGGCTA CCAGTATCTC CGTGTCTAAC 
TGTCAGGTTC AGGAGAATGT CGACATCAGC AGCGTTTACC AGATCTTTGC GGACGAGGTG 
CTTGGTTCTG GCCAGTTTGG CATTGTTTAT GGAGGAAAAC ACAGAAAGAC GGGAAGGGAT 
GTGGCTATTA AAGTGATTGA TAAAATGAGG TTCCCCACAA AGCAAGAGAG TCAGCTCCGG 
AACGAAGTGG CCATTTTACA GAATCTGCAC CATCCTGGGA TTGTAAACCT GGAGTGTATG 
TTTGAAACCC CAGAACGAGT CTTCGTGGTG ATGGAAAAGC TCCATGGAGA TATGTTGGAA 
ATGATTCTGT CCAGTGAAAA GAGTCGACTT CCAGAACGGA TTACCAAATT CATGGTCACA 
CAGATACTTG TTGCCTTGAG GAATTTACAT TTCAAGAATA TTGTGCACTG TGATTTAAAG 
CCCGAAAATG TGCTGCTTGC ATCCGCAGAG CCATTTCCTC AGGTGAAGCT GTGTGACTTT 
GGGTTTGCCC GCATCATTGG TGAGAAGTCG TTCCGGAGGT CAGTGGTAGG AACTCCAGCC 
TACTTAGCCC CTGAGGTTCT CAGGAGCAAA GGCTACAACC GTTCTCTAGA CATGTGGTCG 
GTGGGCGTCA TCGTTTATGT GAGCCTCAGT GGCACATTCC CATTCAATGA AGATGAAGAT 
ATAAATGACC AAATCCAAAA CGCTGCGTTT ATGTACCCAC CAAATCCATG GCGAGAAATT 
TCCAGTGAAG CAATTGACTT GATAAACAAC TTGCTTCAAG TGAAGATGAG AAAACGATAC 
AGTGTTGACA AATCTCTCAG TCATCCTTGG CTACAGGATT ATCAGACTTG GCTTGACCTC 
AGAGAATTTG AAACTCGCAT TGGAGAACGT TACATTACAC ACGAAAGTGA TGATGCTCGC 
TGGGAAATAC ACGCATACAC GCACAATCTG GAATACCCAA AGCATTTCAT TATGGCCCCC 
AACCCAGACG ACATGGAGGA GGATCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083515.2
CDS	763..3432
Translation	MSANNSPPSAQKSVFPATVSAVLPAPSPCSSPKTGLSARLSNGSFSAPSLTNSRGSVHTVSFLLQIGLTRESVTIEAQELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRHDMNSENILQLITSADEIHEGDLVEVVLSALATVEDFQIRPHALYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSNVSLPGPGLSVPRPLQPECVPLLSEESHTHQEPSKRIPSWSGRPIWMEKMVMCRVKVPHTFAVHSYGRPTICQYCKRLLKGLFRQGMQCKDCKFNCHKRCASKVPRDCLGEVTFNGEPCSVGTDADMPMDIDSSDVNSDGSRGLDDSEEPSPPEDKMFFLDPTDLDVERDEETVKTISPSTSNNIPLMRVVQSIKHTKRRSSTVVKEGWMVHYTSRDNLRKRHYWRLDSKCLTLFQNESGSKYYKEIPLSEILRVSSPQDFTSISQGSNPHCFEIITDTVVYFVGENNGSSSHNPVLAATGVGLDVAQSWEKAIRQALMPVTPQASVCTSPGQGKDHNLATSISVSNCQVQENVDISSVYQIFADEVLGSGQFGIVYGGKHRKTGRDVAIKVIDKMRFPTKQESQLRNEVAILQNLHHPGIVNLECMFETPERVFVVMEKLHGDMLEMILSSEKSRLPERITKFMVTQILVALRNLHFKNIVHCDLKPENVLLASAEPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRSKGYNRSLDMWSVGVIVYVSLSGTFPFNEDEDINDQIQNAAFMYPPNPWREISSEAIDLINNLLQVKMRKRYSVDKSLSHPWLQDYQTWLDLREFETRIGERYITHESDDARWEIHAYTHNLEYPKHFIMAPNPDDMEEDP

Target ORF information:

RefSeq Version	NM_029239.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase D3 (Prkd3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029239.3

ORF Insert Sequence:

ATGTCTGCAA ATAATTCCCC TCCATCAGCC CAGAAATCTG TGTTCCCTGC AACTGTTTCT 
GCTGTGCTTC CAGCTCCTTC TCCGTGTTCA AGTCCCAAAA CAGGACTCTC AGCTCGGCTC 
TCTAATGGCA GCTTCAGTGC CCCGTCACTC ACAAACTCTA GAGGCTCAGT GCACACAGTT 
TCATTTCTAC TTCAGATTGG CCTCACACGA GAGAGTGTTA CCATCGAGGC CCAGGAACTG 
TCTCTCTCTG CTGTCAAAGA CCTCGTGTGC TCCATTGTTT ATCAGAAGTT TCCAGAGTGT 
GGATTCTTTG GCATGTATGA TAAAATTCTT CTCTTTCGCC ACGACATGAA CTCAGAAAAC 
ATTTTGCAGC TGATTACCTC AGCAGATGAA ATACATGAAG GGGACCTGGT AGAAGTTGTC 
CTGTCAGCTT TGGCCACAGT GGAAGACTTC CAGATCCGCC CTCACGCTCT CTATGTACAT 
TCTTACAAAG CACCTACTTT TTGTGATTAC TGTGGTGAAA TGCTCTGGGG ACTGGTACGT 
CAAGGATTAA AATGTGAAGG CTGTGGATTA AATTATCATA AACGATGTGC CTTCAAGATT 
CCAAATAACT GCAGTGGAGT AAGAAAGAGA CGTCTGTCAA ATGTATCTCT GCCAGGACCT 
GGCCTCTCAG TTCCAAGACC CCTGCAGCCT GAGTGTGTGC CCCTTCTCAG TGAAGAGTCA 
CATACTCACC AAGAGCCCAG TAAGAGAATC CCGTCGTGGA GTGGTCGTCC GATCTGGATG 
GAAAAGATGG TAATGTGCAG GGTCAAAGTC CCTCACACAT TTGCTGTCCA CTCTTATGGC 
CGCCCGACCA TATGCCAGTA CTGCAAGCGC CTGCTGAAAG GCCTCTTCCG CCAAGGGATG 
CAGTGTAAAG ATTGCAAATT CAACTGCCAT AAACGCTGTG CATCCAAAGT ACCAAGAGAC 
TGCCTTGGCG AAGTTACTTT CAATGGAGAG CCGTGCAGCG TGGGGACTGA TGCAGACATG 
CCCATGGATA TTGACAGTAG TGATGTGAAC AGTGATGGCA GTAGGGGCTT GGATGACTCA 
GAGGAGCCGT CCCCGCCCGA AGACAAGATG TTCTTCCTCG ACCCGACTGA TCTCGACGTG 
GAGCGAGATG AGGAGACTGT TAAGACAATC AGTCCATCCA CCAGCAATAA TATCCCGCTG 
ATGAGGGTGG TGCAGTCCAT CAAGCACACA AAGAGGAGGA GCAGCACAGT TGTGAAGGAA 
GGGTGGATGG TCCACTACAC CAGCAGGGAC AACCTGAGAA AGAGACATTA TTGGAGACTT 
GACAGTAAAT GTCTAACATT GTTTCAAAAT GAATCTGGAT CAAAGTATTA TAAGGAAATT 
CCACTTTCAG AAATTCTCCG TGTATCTTCA CCGCAAGATT TCACCAGCAT TTCACAAGGC 
AGTAACCCAC ACTGTTTTGA AATCATCACC GATACCGTGG TATACTTTGT TGGTGAGAAC 
AATGGGAGCA GCTCTCATAA TCCTGTTCTC GCTGCCACTG GAGTGGGACT AGATGTGGCG 
CAGAGCTGGG AAAAAGCAAT TCGCCAGGCC CTCATGCCTG TCACTCCTCA AGCAAGTGTT 
TGCACTTCTC CGGGGCAAGG GAAGGACCAC AATCTGGCTA CCAGTATCTC CGTGTCTAAC 
TGTCAGGTTC AGGAGAATGT CGACATCAGC AGCGTTTACC AGATCTTTGC GGACGAGGTG 
CTTGGTTCTG GCCAGTTTGG CATTGTTTAT GGAGGAAAAC ACAGAAAGAC GGGAAGGGAT 
GTGGCTATTA AAGTGATTGA TAAAATGAGG TTCCCCACAA AGCAAGAGAG TCAGCTCCGG 
AACGAAGTGG CCATTTTACA GAATCTGCAC CATCCTGGGA TTGTAAACCT GGAGTGTATG 
TTTGAAACCC CAGAACGAGT CTTCGTGGTG ATGGAAAAGC TCCATGGAGA TATGTTGGAA 
ATGATTCTGT CCAGTGAAAA GAGTCGACTT CCAGAACGGA TTACCAAATT CATGGTCACA 
CAGATACTTG TTGCCTTGAG GAATTTACAT TTCAAGAATA TTGTGCACTG TGATTTAAAG 
CCCGAAAATG TGCTGCTTGC ATCCGCAGAG CCATTTCCTC AGGTGAAGCT GTGTGACTTT 
GGGTTTGCCC GCATCATTGG TGAGAAGTCG TTCCGGAGGT CAGTGGTAGG AACTCCAGCC 
TACTTAGCCC CTGAGGTTCT CAGGAGCAAA GGCTACAACC GTTCTCTAGA CATGTGGTCG 
GTGGGCGTCA TCGTTTATGT GAGCCTCAGT GGCACATTCC CATTCAATGA AGATGAAGAT 
ATAAATGACC AAATCCAAAA CGCTGCGTTT ATGTACCCAC CAAATCCATG GCGAGAAATT 
TCCAGTGAAG CAATTGACTT GATAAACAAC TTGCTTCAAG TGAAGATGAG AAAACGATAC 
AGTGTTGACA AATCTCTCAG TCATCCTTGG CTACAGGATT ATCAGACTTG GCTTGACCTC 
AGAGAATTTG AAACTCGCAT TGGAGAACGT TACATTACAC ACGAAAGTGA TGATGCTCGC 
TGGGAAATAC ACGCATACAC GCACAATCTG GAATACCCAA AGCATTTCAT TATGGCCCCC 
AACCCAGACG ACATGGAGGA GGATCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkd3 ( NM_001171004.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001164475.1 Mus musculus protein kinase D3 (Prkd3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11786
Clone ID Related Accession (Same CDS sequence)	NM_001171004.1
Accession Version	NM_001171004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571587200000
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase D3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC151293.2, BC089164.1 and AC154274.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.105054.1, BC089164.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCAA ATAATTCCCC TCCATCAGCC CAGAAATCTG TGTTCCCTGC AACTGTTTCT 
GCTGTGCTTC CAGCTCCTTC TCCGTGTTCA AGTCCCAAAA CAGGACTCTC AGCTCGGCTC 
TCTAATGGCA GCTTCAGTGC CCCGTCACTC ACAAACTCTA GAGGCTCAGT GCACACAGTT 
TCATTTCTAC TTCAGATTGG CCTCACACGA GAGAGTGTTA CCATCGAGGC CCAGGAACTG 
TCTCTCTCTG CTGTCAAAGA CCTCGTGTGC TCCATTGTTT ATCAGAAGTT TCCAGAGTGT 
GGATTCTTTG GCATGTATGA TAAAATTCTT CTCTTTCGCC ACGACATGAA CTCAGAAAAC 
ATTTTGCAGC TGATTACCTC AGCAGATGAA ATACATGAAG GGGACCTGGT AGAAGTTGTC 
CTGTCAGCTT TGGCCACAGT GGAAGACTTC CAGATCCGCC CTCACGCTCT CTATGTACAT 
TCTTACAAAG CACCTACTTT TTGTGATTAC TGTGGTGAAA TGCTCTGGGG ACTGGTACGT 
CAAGGATTAA AATGTGAAGG CTGTGGATTA AATTATCATA AACGATGTGC CTTCAAGATT 
CCAAATAACT GCAGTGGAGT AAGAAAGAGA CGTCTGTCAA ATGTATCTCT GCCAGGACCT 
GGCCTCTCAG TTCCAAGACC CCTGCAGCCT GAGTGTGTGC CCCTTCTCAG TGAAGAGTCA 
CATACTCACC AAGAGCCCAG TAAGAGAATC CCGTCGTGGA GTGGTCGTCC GATCTGGATG 
GAAAAGATGG TAATGTGCAG GGTCAAAGTC CCTCACACAT TTGCTGTCCA CTCTTATGGC 
CGCCCGACCA TATGCCAGTA CTGCAAGCGC CTGCTGAAAG GCCTCTTCCG CCAAGGGATG 
CAGTGTAAAG ATTGCAAATT CAACTGCCAT AAACGCTGTG CATCCAAAGT ACCAAGAGAC 
TGCCTTGGCG AAGTTACTTT CAATGGAGAG CCGTGCAGCG TGGGGACTGA TGCAGACATG 
CCCATGGATA TTGACAGTAG TGATGTGAAC AGTGATGGCA GTAGGGGCTT GGATGACTCA 
GAGGAGCCGT CCCCGCCCGA AGACAAGATG TTCTTCCTCG ACCCGACTGA TCTCGACGTG 
GAGCGAGATG AGGAGACTGT TAAGACAATC AGTCCATCCA CCAGCAATAA TATCCCGCTG 
ATGAGGGTGG TGCAGTCCAT CAAGCACACA AAGAGGAGGA GCAGCACAGT TGTGAAGGAA 
GGGTGGATGG TCCACTACAC CAGCAGGGAC AACCTGAGAA AGAGACATTA TTGGAGACTT 
GACAGTAAAT GTCTAACATT GTTTCAAAAT GAATCTGGAT CAAAGTATTA TAAGGAAATT 
CCACTTTCAG AAATTCTCCG TGTATCTTCA CCGCAAGATT TCACCAGCAT TTCACAAGGC 
AGTAACCCAC ACTGTTTTGA AATCATCACC GATACCGTGG TATACTTTGT TGGTGAGAAC 
AATGGGAGCA GCTCTCATAA TCCTGTTCTC GCTGCCACTG GAGTGGGACT AGATGTGGCG 
CAGAGCTGGG AAAAAGCAAT TCGCCAGGCC CTCATGCCTG TCACTCCTCA AGCAAGTGTT 
TGCACTTCTC CGGGGCAAGG GAAGGACCAC AAAGATCTGG CTACCAGTAT CTCCGTGTCT 
AACTGTCAGG TTCAGGAGAA TGTCGACATC AGCAGCGTTT ACCAGATCTT TGCGGACGAG 
GTGCTTGGTT CTGGCCAGTT TGGCATTGTT TATGGAGGAA AACACAGAAA GACGGGAAGG 
GATGTGGCTA TTAAAGTGAT TGATAAAATG AGGTTCCCCA CAAAGCAAGA GAGTCAGCTC 
CGGAACGAAG TGGCCATTTT ACAGAATCTG CACCATCCTG GGATTGTAAA CCTGGAGTGT 
ATGTTTGAAA CCCCAGAACG AGTCTTCGTG GTGATGGAAA AGCTCCATGG AGATATGTTG 
GAAATGATTC TGTCCAGTGA AAAGAGTCGA CTTCCAGAAC GGATTACCAA ATTCATGGTC 
ACACAGATAC TTGTTGCCTT GAGGAATTTA CATTTCAAGA ATATTGTGCA CTGTGATTTA 
AAGCCCGAAA ATGTGCTGCT TGCATCCGCA GAGCCATTTC CTCAGGTGAA GCTGTGTGAC 
TTTGGGTTTG CCCGCATCAT TGGTGAGAAG TCGTTCCGGA GGTCAGTGGT AGGAACTCCA 
GCCTACTTAG CCCCTGAGGT TCTCAGGAGC AAAGGCTACA ACCGTTCTCT AGACATGTGG 
TCGGTGGGCG TCATCGTTTA TGTGAGCCTC AGTGGCACAT TCCCATTCAA TGAAGATGAA 
GATATAAATG ACCAAATCCA AAACGCTGCG TTTATGTACC CACCAAATCC ATGGCGAGAA 
ATTTCCAGTG AAGCAATTGA CTTGATAAAC AACTTGCTTC AAGTGAAGAT GAGAAAACGA 
TACAGTGTTG ACAAATCTCT CAGTCATCCT TGGCTACAGG ATTATCAGAC TTGGCTTGAC 
CTCAGAGAAT TTGAAACTCG CATTGGAGAA CGTTACATTA CACACGAAAG TGATGATGCT 
CGCTGGGAAA TACACGCATA CACGCACAAT CTGGAATACC CAAAGCATTT CATTATGGCC 
CCCAACCCAG ACGACATGGA GGAGGATCCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164475.1
CDS	763..3435
Translation	MSANNSPPSAQKSVFPATVSAVLPAPSPCSSPKTGLSARLSNGSFSAPSLTNSRGSVHTVSFLLQIGLTRESVTIEAQELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRHDMNSENILQLITSADEIHEGDLVEVVLSALATVEDFQIRPHALYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSNVSLPGPGLSVPRPLQPECVPLLSEESHTHQEPSKRIPSWSGRPIWMEKMVMCRVKVPHTFAVHSYGRPTICQYCKRLLKGLFRQGMQCKDCKFNCHKRCASKVPRDCLGEVTFNGEPCSVGTDADMPMDIDSSDVNSDGSRGLDDSEEPSPPEDKMFFLDPTDLDVERDEETVKTISPSTSNNIPLMRVVQSIKHTKRRSSTVVKEGWMVHYTSRDNLRKRHYWRLDSKCLTLFQNESGSKYYKEIPLSEILRVSSPQDFTSISQGSNPHCFEIITDTVVYFVGENNGSSSHNPVLAATGVGLDVAQSWEKAIRQALMPVTPQASVCTSPGQGKDHKDLATSISVSNCQVQENVDISSVYQIFADEVLGSGQFGIVYGGKHRKTGRDVAIKVIDKMRFPTKQESQLRNEVAILQNLHHPGIVNLECMFETPERVFVVMEKLHGDMLEMILSSEKSRLPERITKFMVTQILVALRNLHFKNIVHCDLKPENVLLASAEPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRSKGYNRSLDMWSVGVIVYVSLSGTFPFNEDEDINDQIQNAAFMYPPNPWREISSEAIDLINNLLQVKMRKRYSVDKSLSHPWLQDYQTWLDLREFETRIGERYITHESDDARWEIHAYTHNLEYPKHFIMAPNPDDMEEDP

Target ORF information:

RefSeq Version	NM_001171004.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase D3 (Prkd3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171004.1

ORF Insert Sequence:

ATGTCTGCAA ATAATTCCCC TCCATCAGCC CAGAAATCTG TGTTCCCTGC AACTGTTTCT 
GCTGTGCTTC CAGCTCCTTC TCCGTGTTCA AGTCCCAAAA CAGGACTCTC AGCTCGGCTC 
TCTAATGGCA GCTTCAGTGC CCCGTCACTC ACAAACTCTA GAGGCTCAGT GCACACAGTT 
TCATTTCTAC TTCAGATTGG CCTCACACGA GAGAGTGTTA CCATCGAGGC CCAGGAACTG 
TCTCTCTCTG CTGTCAAAGA CCTCGTGTGC TCCATTGTTT ATCAGAAGTT TCCAGAGTGT 
GGATTCTTTG GCATGTATGA TAAAATTCTT CTCTTTCGCC ACGACATGAA CTCAGAAAAC 
ATTTTGCAGC TGATTACCTC AGCAGATGAA ATACATGAAG GGGACCTGGT AGAAGTTGTC 
CTGTCAGCTT TGGCCACAGT GGAAGACTTC CAGATCCGCC CTCACGCTCT CTATGTACAT 
TCTTACAAAG CACCTACTTT TTGTGATTAC TGTGGTGAAA TGCTCTGGGG ACTGGTACGT 
CAAGGATTAA AATGTGAAGG CTGTGGATTA AATTATCATA AACGATGTGC CTTCAAGATT 
CCAAATAACT GCAGTGGAGT AAGAAAGAGA CGTCTGTCAA ATGTATCTCT GCCAGGACCT 
GGCCTCTCAG TTCCAAGACC CCTGCAGCCT GAGTGTGTGC CCCTTCTCAG TGAAGAGTCA 
CATACTCACC AAGAGCCCAG TAAGAGAATC CCGTCGTGGA GTGGTCGTCC GATCTGGATG 
GAAAAGATGG TAATGTGCAG GGTCAAAGTC CCTCACACAT TTGCTGTCCA CTCTTATGGC 
CGCCCGACCA TATGCCAGTA CTGCAAGCGC CTGCTGAAAG GCCTCTTCCG CCAAGGGATG 
CAGTGTAAAG ATTGCAAATT CAACTGCCAT AAACGCTGTG CATCCAAAGT ACCAAGAGAC 
TGCCTTGGCG AAGTTACTTT CAATGGAGAG CCGTGCAGCG TGGGGACTGA TGCAGACATG 
CCCATGGATA TTGACAGTAG TGATGTGAAC AGTGATGGCA GTAGGGGCTT GGATGACTCA 
GAGGAGCCGT CCCCGCCCGA AGACAAGATG TTCTTCCTCG ACCCGACTGA TCTCGACGTG 
GAGCGAGATG AGGAGACTGT TAAGACAATC AGTCCATCCA CCAGCAATAA TATCCCGCTG 
ATGAGGGTGG TGCAGTCCAT CAAGCACACA AAGAGGAGGA GCAGCACAGT TGTGAAGGAA 
GGGTGGATGG TCCACTACAC CAGCAGGGAC AACCTGAGAA AGAGACATTA TTGGAGACTT 
GACAGTAAAT GTCTAACATT GTTTCAAAAT GAATCTGGAT CAAAGTATTA TAAGGAAATT 
CCACTTTCAG AAATTCTCCG TGTATCTTCA CCGCAAGATT TCACCAGCAT TTCACAAGGC 
AGTAACCCAC ACTGTTTTGA AATCATCACC GATACCGTGG TATACTTTGT TGGTGAGAAC 
AATGGGAGCA GCTCTCATAA TCCTGTTCTC GCTGCCACTG GAGTGGGACT AGATGTGGCG 
CAGAGCTGGG AAAAAGCAAT TCGCCAGGCC CTCATGCCTG TCACTCCTCA AGCAAGTGTT 
TGCACTTCTC CGGGGCAAGG GAAGGACCAC AAAGATCTGG CTACCAGTAT CTCCGTGTCT 
AACTGTCAGG TTCAGGAGAA TGTCGACATC AGCAGCGTTT ACCAGATCTT TGCGGACGAG 
GTGCTTGGTT CTGGCCAGTT TGGCATTGTT TATGGAGGAA AACACAGAAA GACGGGAAGG 
GATGTGGCTA TTAAAGTGAT TGATAAAATG AGGTTCCCCA CAAAGCAAGA GAGTCAGCTC 
CGGAACGAAG TGGCCATTTT ACAGAATCTG CACCATCCTG GGATTGTAAA CCTGGAGTGT 
ATGTTTGAAA CCCCAGAACG AGTCTTCGTG GTGATGGAAA AGCTCCATGG AGATATGTTG 
GAAATGATTC TGTCCAGTGA AAAGAGTCGA CTTCCAGAAC GGATTACCAA ATTCATGGTC 
ACACAGATAC TTGTTGCCTT GAGGAATTTA CATTTCAAGA ATATTGTGCA CTGTGATTTA 
AAGCCCGAAA ATGTGCTGCT TGCATCCGCA GAGCCATTTC CTCAGGTGAA GCTGTGTGAC 
TTTGGGTTTG CCCGCATCAT TGGTGAGAAG TCGTTCCGGA GGTCAGTGGT AGGAACTCCA 
GCCTACTTAG CCCCTGAGGT TCTCAGGAGC AAAGGCTACA ACCGTTCTCT AGACATGTGG 
TCGGTGGGCG TCATCGTTTA TGTGAGCCTC AGTGGCACAT TCCCATTCAA TGAAGATGAA 
GATATAAATG ACCAAATCCA AAACGCTGCG TTTATGTACC CACCAAATCC ATGGCGAGAA 
ATTTCCAGTG AAGCAATTGA CTTGATAAAC AACTTGCTTC AAGTGAAGAT GAGAAAACGA 
TACAGTGTTG ACAAATCTCT CAGTCATCCT TGGCTACAGG ATTATCAGAC TTGGCTTGAC 
CTCAGAGAAT TTGAAACTCG CATTGGAGAA CGTTACATTA CACACGAAAG TGATGATGCT 
CGCTGGGAAA TACACGCATA CACGCACAAT CTGGAATACC CAAAGCATTT CATTATGGCC 
CCCAACCCAG ACGACATGGA GGAGGATCCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkd3 ( NM_001171005.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001164476.1 Mus musculus protein kinase D3 (Prkd3), transcript variant 3, mRNA.

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CloneID	OMu11790
Clone ID Related Accession (Same CDS sequence)	NM_001171005.1
Accession Version	NM_001171005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571500800000
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase D3 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC151293.2, AK030058.1 and AC154274.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK030058.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTCCAG AGTGTGGATT CTTTGGCATG TATGATAAAA TTCTTCTCTT TCGCCACGAC 
ATGAACTCAG AAAACATTTT GCAGCTGATT ACCTCAGCAG ATGAAATACA TGAAGGGGAC 
CTGGTAGAAG TTGTCCTGTC AGCTTTGGCC ACAGTGGAAG ACTTCCAGAT CCGCCCTCAC 
GCTCTCTATG TACATTCTTA CAAAGCACCT ACTTTTTGTG ATTACTGTGG TGAAATGCTC 
TGGGGACTGG TACGTCAAGG ATTAAAATGT GAAGGCTGTG GATTAAATTA TCATAAACGA 
TGTGCCTTCA AGATTCCAAA TAACTGCAGT GGAGTAAGAA AGAGACGTCT GTCAAATGTA 
TCTCTGCCAG GACCTGGCCT CTCAGTTCCA AGACCCCTGC AGCCTGAGTG TGTGCCCCTT 
CTCAGTGAAG AGTCACATAC TCACCAAGAG CCCAGTAAGA GAATCCCGTC GTGGAGTGGT 
CGTCCGATCT GGATGGAAAA GATGGTAATG TGCAGGGTCA AAGTCCCTCA CACATTTGCT 
GTCCACTCTT ATGGCCGCCC GACCATATGC CAGTACTGCA AGCGCCTGCT GAAAGGCCTC 
TTCCGCCAAG GGATGCAGTG TAAAGATTGC AAATTCAACT GCCATAAACG CTGTGCATCC 
AAAGTACCAA GAGACTGCCT TGGCGAAGTT ACTTTCAATG GAGAGCCGTG CAGCGTGGGG 
ACTGATGCAG ACATGCCCAT GGATATTGAC AGTAGTGATG TGAACAGTGA TGGCAGTAGG 
GGCTTGGATG ACTCAGAGGA GCCGTCCCCG CCCGAAGACA AGATGTTCTT CCTCGACCCG 
ACTGATCTCG ACGTGGAGCG AGATGAGGAG ACTGTTAAGA CAATCAGTCC ATCCACCAGC 
AATAATATCC CGCTGATGAG GGTGGTGCAG TCCATCAAGC ACACAAAGAG GAGGAGCAGC 
ACAGTTGTGA AGGAAGGGTG GATGGTCCAC TACACCAGCA GGGACAACCT GAGAAAGAGA 
CATTATTGGA GACTTGACAG TAAATGTCTA ACATTGTTTC AAAATGAATC TGGATCAAAG 
TATTATAAGG AAATTCCACT TTCAGAAATT CTCCGTGTAT CTTCACCGCA AGATTTCACC 
AGCATTTCAC AAGGCAGTAA CCCACACTGT TTTGAAATCA TCACCGATAC CGTGGTATAC 
TTTGTTGGTG AGAACAATGG GAGCAGCTCT CATAATCCTG TTCTCGCTGC CACTGGAGTG 
GGACTAGATG TGGCGCAGAG CTGGGAAAAA GCAATTCGCC AGGCCCTCAT GCCTGTCACT 
CCTCAAGCAA GTGTTTGCAC TTCTCCGGGG CAAGGGAAGG ACCACAAAGA TCTGGCTACC 
AGTATCTCCG TGTCTAACTG TCAGGTTCAG GAGAATGTCG ACATCAGCAG CGTTTACCAG 
ATCTTTGCGG ACGAGGTGCT TGGTTCTGGC CAGTTTGGCA TTGTTTATGG AGGAAAACAC 
AGAAAGACGG GAAGGGATGT GGCTATTAAA GTGATTGATA AAATGAGGTT CCCCACAAAG 
CAAGAGAGTC AGCTCCGGAA CGAAGTGGCC ATTTTACAGA ATCTGCACCA TCCTGGGATT 
GTAAACCTGG AGTGTATGTT TGAAACCCCA GAACGAGTCT TCGTGGTGAT GGAAAAGCTC 
CATGGAGATA TGTTGGAAAT GATTCTGTCC AGTGAAAAGA GTCGACTTCC AGAACGGATT 
ACCAAATTCA TGGTCACACA GATACTTGTT GCCTTGAGGA ATTTACATTT CAAGAATATT 
GTGCACTGTG ATTTAAAGCC CGAAAATGTG CTGCTTGCAT CCGCAGAGCC ATTTCCTCAG 
GTGAAGCTGT GTGACTTTGG GTTTGCCCGC ATCATTGGTG AGAAGTCGTT CCGGAGGTCA 
GTGGTAGGAA CTCCAGCCTA CTTAGCCCCT GAGGTTCTCA GGAGCAAAGG CTACAACCGT 
TCTCTAGACA TGTGGTCGGT GGGCGTCATC GTTTATGTGA GCCTCAGTGG CACATTCCCA 
TTCAATGAAG ATGAAGATAT AAATGACCAA ATCCAAAACG CTGCGTTTAT GTACCCACCA 
AATCCATGGC GAGAAATTTC CAGTGAAGCA ATTGACTTGA TAAACAACTT GCTTCAAGTG 
AAGATGAGAA AACGATACAG TGTTGACAAA TCTCTCAGTC ATCCTTGGCT ACAGGATTAT 
CAGACTTGGC TTGACCTCAG AGAATTTGAA ACTCGCATTG GAGAACGTTA CATTACACAC 
GAAAGTGATG ATGCTCGCTG GGAAATACAC GCATACACGC ACAATCTGGA ATACCCAAAG 
CATTTCATTA TGGCCCCCAA CCCAGACGAC ATGGAGGAGG ATCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164476.1
CDS	244..2631
Translation	MFPECGFFGMYDKILLFRHDMNSENILQLITSADEIHEGDLVEVVLSALATVEDFQIRPHALYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSNVSLPGPGLSVPRPLQPECVPLLSEESHTHQEPSKRIPSWSGRPIWMEKMVMCRVKVPHTFAVHSYGRPTICQYCKRLLKGLFRQGMQCKDCKFNCHKRCASKVPRDCLGEVTFNGEPCSVGTDADMPMDIDSSDVNSDGSRGLDDSEEPSPPEDKMFFLDPTDLDVERDEETVKTISPSTSNNIPLMRVVQSIKHTKRRSSTVVKEGWMVHYTSRDNLRKRHYWRLDSKCLTLFQNESGSKYYKEIPLSEILRVSSPQDFTSISQGSNPHCFEIITDTVVYFVGENNGSSSHNPVLAATGVGLDVAQSWEKAIRQALMPVTPQASVCTSPGQGKDHKDLATSISVSNCQVQENVDISSVYQIFADEVLGSGQFGIVYGGKHRKTGRDVAIKVIDKMRFPTKQESQLRNEVAILQNLHHPGIVNLECMFETPERVFVVMEKLHGDMLEMILSSEKSRLPERITKFMVTQILVALRNLHFKNIVHCDLKPENVLLASAEPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRSKGYNRSLDMWSVGVIVYVSLSGTFPFNEDEDINDQIQNAAFMYPPNPWREISSEAIDLINNLLQVKMRKRYSVDKSLSHPWLQDYQTWLDLREFETRIGERYITHESDDARWEIHAYTHNLEYPKHFIMAPNPDDMEEDP

Target ORF information:

RefSeq Version	NM_001171005.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase D3 (Prkd3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171005.1

ORF Insert Sequence:

ATGTTTCCAG AGTGTGGATT CTTTGGCATG TATGATAAAA TTCTTCTCTT TCGCCACGAC 
ATGAACTCAG AAAACATTTT GCAGCTGATT ACCTCAGCAG ATGAAATACA TGAAGGGGAC 
CTGGTAGAAG TTGTCCTGTC AGCTTTGGCC ACAGTGGAAG ACTTCCAGAT CCGCCCTCAC 
GCTCTCTATG TACATTCTTA CAAAGCACCT ACTTTTTGTG ATTACTGTGG TGAAATGCTC 
TGGGGACTGG TACGTCAAGG ATTAAAATGT GAAGGCTGTG GATTAAATTA TCATAAACGA 
TGTGCCTTCA AGATTCCAAA TAACTGCAGT GGAGTAAGAA AGAGACGTCT GTCAAATGTA 
TCTCTGCCAG GACCTGGCCT CTCAGTTCCA AGACCCCTGC AGCCTGAGTG TGTGCCCCTT 
CTCAGTGAAG AGTCACATAC TCACCAAGAG CCCAGTAAGA GAATCCCGTC GTGGAGTGGT 
CGTCCGATCT GGATGGAAAA GATGGTAATG TGCAGGGTCA AAGTCCCTCA CACATTTGCT 
GTCCACTCTT ATGGCCGCCC GACCATATGC CAGTACTGCA AGCGCCTGCT GAAAGGCCTC 
TTCCGCCAAG GGATGCAGTG TAAAGATTGC AAATTCAACT GCCATAAACG CTGTGCATCC 
AAAGTACCAA GAGACTGCCT TGGCGAAGTT ACTTTCAATG GAGAGCCGTG CAGCGTGGGG 
ACTGATGCAG ACATGCCCAT GGATATTGAC AGTAGTGATG TGAACAGTGA TGGCAGTAGG 
GGCTTGGATG ACTCAGAGGA GCCGTCCCCG CCCGAAGACA AGATGTTCTT CCTCGACCCG 
ACTGATCTCG ACGTGGAGCG AGATGAGGAG ACTGTTAAGA CAATCAGTCC ATCCACCAGC 
AATAATATCC CGCTGATGAG GGTGGTGCAG TCCATCAAGC ACACAAAGAG GAGGAGCAGC 
ACAGTTGTGA AGGAAGGGTG GATGGTCCAC TACACCAGCA GGGACAACCT GAGAAAGAGA 
CATTATTGGA GACTTGACAG TAAATGTCTA ACATTGTTTC AAAATGAATC TGGATCAAAG 
TATTATAAGG AAATTCCACT TTCAGAAATT CTCCGTGTAT CTTCACCGCA AGATTTCACC 
AGCATTTCAC AAGGCAGTAA CCCACACTGT TTTGAAATCA TCACCGATAC CGTGGTATAC 
TTTGTTGGTG AGAACAATGG GAGCAGCTCT CATAATCCTG TTCTCGCTGC CACTGGAGTG 
GGACTAGATG TGGCGCAGAG CTGGGAAAAA GCAATTCGCC AGGCCCTCAT GCCTGTCACT 
CCTCAAGCAA GTGTTTGCAC TTCTCCGGGG CAAGGGAAGG ACCACAAAGA TCTGGCTACC 
AGTATCTCCG TGTCTAACTG TCAGGTTCAG GAGAATGTCG ACATCAGCAG CGTTTACCAG 
ATCTTTGCGG ACGAGGTGCT TGGTTCTGGC CAGTTTGGCA TTGTTTATGG AGGAAAACAC 
AGAAAGACGG GAAGGGATGT GGCTATTAAA GTGATTGATA AAATGAGGTT CCCCACAAAG 
CAAGAGAGTC AGCTCCGGAA CGAAGTGGCC ATTTTACAGA ATCTGCACCA TCCTGGGATT 
GTAAACCTGG AGTGTATGTT TGAAACCCCA GAACGAGTCT TCGTGGTGAT GGAAAAGCTC 
CATGGAGATA TGTTGGAAAT GATTCTGTCC AGTGAAAAGA GTCGACTTCC AGAACGGATT 
ACCAAATTCA TGGTCACACA GATACTTGTT GCCTTGAGGA ATTTACATTT CAAGAATATT 
GTGCACTGTG ATTTAAAGCC CGAAAATGTG CTGCTTGCAT CCGCAGAGCC ATTTCCTCAG 
GTGAAGCTGT GTGACTTTGG GTTTGCCCGC ATCATTGGTG AGAAGTCGTT CCGGAGGTCA 
GTGGTAGGAA CTCCAGCCTA CTTAGCCCCT GAGGTTCTCA GGAGCAAAGG CTACAACCGT 
TCTCTAGACA TGTGGTCGGT GGGCGTCATC GTTTATGTGA GCCTCAGTGG CACATTCCCA 
TTCAATGAAG ATGAAGATAT AAATGACCAA ATCCAAAACG CTGCGTTTAT GTACCCACCA 
AATCCATGGC GAGAAATTTC CAGTGAAGCA ATTGACTTGA TAAACAACTT GCTTCAAGTG 
AAGATGAGAA AACGATACAG TGTTGACAAA TCTCTCAGTC ATCCTTGGCT ACAGGATTAT 
CAGACTTGGC TTGACCTCAG AGAATTTGAA ACTCGCATTG GAGAACGTTA CATTACACAC 
GAAAGTGATG ATGCTCGCTG GGAAATACAC GCATACACGC ACAATCTGGA ATACCCAAAG 
CATTTCATTA TGGCCCCCAA CCCAGACGAC ATGGAGGAGG ATCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein kinase D regulates positive selection of CD4
Nature communications712756(2016 Sep)
Ishikawa E,Kosako H,Yasuda T,Ohmuraya M,Araki K,Kurosaki T,Saito T,Yamasaki S

PKD3 deficiency causes alterations in microtubule dynamics during the cell cycle.
Cell cycle (Georgetown, Tex.)15(14)1844-54(2016 07)
Zhang T,Braun U,Leitges M

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Immunity43(4)690-702(2015 Oct)
Ray JP,Staron MM,Shyer JA,Ho PC,Marshall HD,Gray SM,Laidlaw BJ,Araki K,Ahmed R,Kaech SM,Craft J

Protein kinase D-HDAC5 signaling regulates erythropoiesis and contributes to erythropoietin cross-talk with GATA1.
Blood120(20)4219-28(2012 Nov)
Delehanty LL,Bullock GC,Goldfarb AN

Inducible silencing of protein kinase D3 inhibits secretion of tumor-promoting factors in prostate cancer.
Molecular cancer therapeutics11(7)1389-99(2012 Jul)
LaValle CR,Zhang L,Xu S,Eiseman JL,Wang QJ

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Prkd3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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