Als2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Als2
Entrez Gene ID	74018
Full Name	alsin Rho guanine nucleotide exchange factor
Synonyms	3222402C23Rik,9430073A21Rik,Als2cr6,Alsin,mKIAA1563
General protein information	Preferred Names alsin Rho guanine nucleotide exchange factor Names alsin amyotrophic lateral sclerosis 2 (juvenile) homolog amyotrophic lateral sclerosis 2 protein homolog amyotrophic lateral sclerosis protein 2 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C1.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_146109.3	NP_666221.1	alsin isoform 2
NM_028717.6	NP_082993.4	alsin isoform 1
NM_001159948.2	NP_001153420.2	alsin isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ALS2	XP_003640234.1
Danio rerio (zebrafish)	als2b	XP_002662342.2
Homo sapiens (human)	ALS2	NP_065970.2
Bos taurus (cattle)	ALS2	XP_005202742.1
Rattus norvegicus (Norway rat)	Als2	NP_001013431.1
Pan troglodytes (chimpanzee)	ALS2	NP_001073389.1
Xenopus tropicalis (tropical clawed frog)	als2	XP_004917813.1
Macaca mulatta (Rhesus monkey)	ALS2	XP_001091562.2
Mus musculus (house mouse)	Als2	NP_001153420.2
Gallus gallus (chicken)	ALS2	XP_421940.2

Gene Ontology
Bibliography

Related articles in PubMed

Functional links between SQSTM1 and ALS2 in the pathogenesis of ALS: cumulative impact on the protection against mutant SOD1-mediated motor dysfunction in mice.
Hadano S, Mitsui S, Pan L, Otomo A, Kubo M, Sato K, Ono S, Onodera W, Abe K, Chen X, Koike M, Uchiyama Y, Aoki M, Warabi E, Yamamoto M, Ishii T, Yanagawa T, Shang HF, Yoshii F
Human molecular genetics25(15)3321-3340(2016 08)

Absence of alsin function leads to corticospinal motor neuron vulnerability via novel disease mechanisms.
Gautam M, Jara JH, Sekerkova G, Yasvoina MV, Martina M, Özdinler PH
Human molecular genetics25(6)1074-87(2016 Mar)

Are alsin and spartin novel interaction partners?
Çobanoğlu G, Ozansoy M, Başak AN
Biochemical and biophysical research communications427(1)1-4(2012 Oct)

Different human copper-zinc superoxide dismutase mutants, SOD1G93A and SOD1H46R, exert distinct harmful effects on gross phenotype in mice.
Pan L, Yoshii Y, Otomo A, Ogawa H, Iwasaki Y, Shang HF, Hadano S
PloS one7(3)e33409(2012)

Alsin and SOD1(G93A) proteins regulate endosomal reactive oxygen species production by glial cells and proinflammatory pathways responsible for neurotoxicity.
Li Q, Spencer NY, Pantazis NJ, Engelhardt JF
The Journal of biological chemistry286(46)40151-62(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Als2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Als2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16295	NM_146109.3 Latest version!	Mus musculus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu15317	NM_001159948.2 Latest version!	Mus musculus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu15317	NM_028717.6 Latest version!	Mus musculus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Als2 ( NM_146109.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666221.1 Mus musculus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16295
Clone ID Related Accession (Same CDS sequence)	NM_146109.3
Accession Version	NM_146109.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	alsin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153652.3, BC046828.1 and CD543795.1. On May 19, 2009 this sequence version replaced NM_146109.2. Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR, and 3' coding sequence compared to variant 3. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031479.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCAA AGAAGAAAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGTGGA 
AAGACAGTTC TTCAGGCAGC CCTTGGTGTG AGGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AAAAGTGAAT CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTGAC AGAGAGCGGG GTGGTGTACA TGTGGGGAGA GAATGCTGCC 
GGGCAGTGTG CGGTAGCTAA CCAGCAGTAT GTGCCGGAGC CGAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCGTC ATTAGCAGTT AGGATTCTGC AATTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCACT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGCCTCATCA CCACCACTTT CCCAGTGACA AAGCCACAGA AGGTGGAACA CCTTGCTGGA 
CGCGTGGTGC TCCAGGTGGC CTGCGGTGCA TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCTCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCATGTGATA ATATCGGACA GCCATTGCTG CCCTTTAGGT 
GTGACATTGT CCGAGTCTCA AGCAGAAAAG CATGCCAGCC CTGCTCCCAG CCCTCACCCA 
GAGGCACTGG ATGAGCAGGG AGAGGTGTTT GAGAACACGG TGGTAGAAGC TGAACTGAAC 
ATGGGAAGCA GTCAGACCAC AAGTGGCAGT GCCATTTCCA CCCAGCAGAA CGTCGTGGGA 
ACAGCTGAAG TGTCTTCTGC CAGAACAGCT CCGTCATACC CAGACACCCA TGCGGTAACT 
GCATACCTGC AGAAGCTGTC AGAGCATTCG ATGAGGGAGA ACCATGAGCC TGGAGAAAAG 
CCACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTCAGA 
GTGGCTGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTACAGC 
ACTGCCCCCT GCGAGACGGC AGCTCAGTCG GGCAGTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCC GAGAAGAGCA GGTGAAACAG GAGTCACTGC AAGGGAAGAA AAGCTCAAGT 
CTCATGGACA TCAGAGAGGA GGAGTTGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGG 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCTG CGAGGGTGAA AACTCGGACA 
GTGGTTCTGA CTCCCACATA CAGTGGAGAA GCAGATGCCC TTCTGCCTTC CCTGAGGACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTAG GGCACGGCGA CGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTAATCCA CCTGGAGGCG 
GGCGGCTCCC ACTCCCTCGC ACTCACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTTGGTC AGCTTGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCAAAGGTT 
AGCAGTGAAA ATGGAGTCTG GAGTGTAGCT GCAGGCCAAG ATTATTCCTT GTTTTTAGTG 
GACACGGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GACAGGACCG TGCAGAAGGT 
GATACCCTGC CAGAGAATCC CAGTGGTACA AAGACTCCAG TACTTCTCTC CTGTAGTAAG 
CTTGGATACA TAAGCAGAGT AACAGCAGGA AAAGATAGCT ATCTAGCCTT GGTGGATAAG 
AACATCATGG GATACATCGC CAGTCTCCAT GAGTTGGCTT CTACAGAAAG ACGGTTTTAC 
TCAAAACTGA GCGAAATCAA ATCACAGATA CTTAGGCCTC TTCTCAGTTT AGAAAATTTG 
GGCACAGTGA CCACTGTCCA GCTGTTGCAG GAAGTTGCCA GCCGGTTCAG CAAGTTGTGT 
TACCTCATTG GGCAGCATGG AGCCTCACTA AGCAGCTACC TACAGGGCAT GAAGGAAGCC 
AGCAGCCTGG TCATCATGAA GCACTCAAGT CTTTTCCTGG ACAGCTACAC AGAGTACTGC 
ACATCAGTTT CAAATTTCCT GGTTATGGGA GGATTCCAGC TTCTTGCTAA GCCTGCCATT 
GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC 
ACTCAGTTGA TGGAAATCCT GAACATGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT 
TATGCAAAAG TTTTGCTAAA GCTTGCCACT TGCTTTGAAG TGGTAGGTTT CGTGTGTGCC 
CCTCCAAATA CTCGGGAAGC CAAGAGTCAA AGTAGTTTGT TTGCATTAAG TCTCCTTAAG 
ATGTTGGGCC CCGGGGCTGG TGAGATGGCT CAGTGGGTAA GAGCACCTGA CTGCTCTTCC 
GAAGGTCCGG AGTTCAAATC CCAGCAACCA CATGGTGGCT CACAACCACC CGTAATGAGA 
TCTGACGCCC TCTTCTGGAG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666221.1
CDS	136..2922
Translation	MDSKKKSSTEAEGSKERGLVHVWQAGSFSLTPERLPGWGGKTVLQAALGVRHGVLLTEDGEVYSFGTLPWKSESAEICPSSPLLESALVGHHVITVATGSFHSGAVTESGVVYMWGENAAGQCAVANQQYVPEPSPVSISDSETSPSLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPPQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEKHASPAPSPHPEALDEQGEVFENTVVEAELNMGSSQTTSGSAISTQQNVVGTAEVSSARTAPSYPDTHAVTAYLQKLSEHSMRENHEPGEKPPQVQPLVEEAVPDLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCETAAQSGSASTGPESLKDLREEQVKQESLQGKKSSSLMDIREEELEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGSHSLALTAKSQVYSWGSNTFGQLGHSEFPTTVPRLSKVSSENGVWSVAAGQDYSLFLVDTEDFQPGLYYSGRQDRAEGDTLPENPSGTKTPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELASTERRFYSKLSEIKSQILRPLLSLENLGTVTTVQLLQEVASRFSKLCYLIGQHGASLSSYLQGMKEASSLVIMKHSSLFLDSYTEYCTSVSNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNMLFFLPIRRLHNYAKVLLKLATCFEVVGFVCAPPNTREAKSQSSLFALSLLKMLGPGAGEMAQWVRAPDCSSEGPEFKSQQPHGGSQPPVMRSDALFWSF

Target ORF information:

RefSeq Version	NM_146109.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146109.3

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGTGGA 
AAGACAGTTC TTCAGGCAGC CCTTGGTGTG AGGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AAAAGTGAAT CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTGAC AGAGAGCGGG GTGGTGTACA TGTGGGGAGA GAATGCTGCC 
GGGCAGTGTG CGGTAGCTAA CCAGCAGTAT GTGCCGGAGC CGAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCGTC ATTAGCAGTT AGGATTCTGC AATTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCACT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGCCTCATCA CCACCACTTT CCCAGTGACA AAGCCACAGA AGGTGGAACA CCTTGCTGGA 
CGCGTGGTGC TCCAGGTGGC CTGCGGTGCA TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCTCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCATGTGATA ATATCGGACA GCCATTGCTG CCCTTTAGGT 
GTGACATTGT CCGAGTCTCA AGCAGAAAAG CATGCCAGCC CTGCTCCCAG CCCTCACCCA 
GAGGCACTGG ATGAGCAGGG AGAGGTGTTT GAGAACACGG TGGTAGAAGC TGAACTGAAC 
ATGGGAAGCA GTCAGACCAC AAGTGGCAGT GCCATTTCCA CCCAGCAGAA CGTCGTGGGA 
ACAGCTGAAG TGTCTTCTGC CAGAACAGCT CCGTCATACC CAGACACCCA TGCGGTAACT 
GCATACCTGC AGAAGCTGTC AGAGCATTCG ATGAGGGAGA ACCATGAGCC TGGAGAAAAG 
CCACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTCAGA 
GTGGCTGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTACAGC 
ACTGCCCCCT GCGAGACGGC AGCTCAGTCG GGCAGTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCC GAGAAGAGCA GGTGAAACAG GAGTCACTGC AAGGGAAGAA AAGCTCAAGT 
CTCATGGACA TCAGAGAGGA GGAGTTGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGG 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCTG CGAGGGTGAA AACTCGGACA 
GTGGTTCTGA CTCCCACATA CAGTGGAGAA GCAGATGCCC TTCTGCCTTC CCTGAGGACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTAG GGCACGGCGA CGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTAATCCA CCTGGAGGCG 
GGCGGCTCCC ACTCCCTCGC ACTCACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTTGGTC AGCTTGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCAAAGGTT 
AGCAGTGAAA ATGGAGTCTG GAGTGTAGCT GCAGGCCAAG ATTATTCCTT GTTTTTAGTG 
GACACGGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GACAGGACCG TGCAGAAGGT 
GATACCCTGC CAGAGAATCC CAGTGGTACA AAGACTCCAG TACTTCTCTC CTGTAGTAAG 
CTTGGATACA TAAGCAGAGT AACAGCAGGA AAAGATAGCT ATCTAGCCTT GGTGGATAAG 
AACATCATGG GATACATCGC CAGTCTCCAT GAGTTGGCTT CTACAGAAAG ACGGTTTTAC 
TCAAAACTGA GCGAAATCAA ATCACAGATA CTTAGGCCTC TTCTCAGTTT AGAAAATTTG 
GGCACAGTGA CCACTGTCCA GCTGTTGCAG GAAGTTGCCA GCCGGTTCAG CAAGTTGTGT 
TACCTCATTG GGCAGCATGG AGCCTCACTA AGCAGCTACC TACAGGGCAT GAAGGAAGCC 
AGCAGCCTGG TCATCATGAA GCACTCAAGT CTTTTCCTGG ACAGCTACAC AGAGTACTGC 
ACATCAGTTT CAAATTTCCT GGTTATGGGA GGATTCCAGC TTCTTGCTAA GCCTGCCATT 
GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC 
ACTCAGTTGA TGGAAATCCT GAACATGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT 
TATGCAAAAG TTTTGCTAAA GCTTGCCACT TGCTTTGAAG TGGTAGGTTT CGTGTGTGCC 
CCTCCAAATA CTCGGGAAGC CAAGAGTCAA AGTAGTTTGT TTGCATTAAG TCTCCTTAAG 
ATGTTGGGCC CCGGGGCTGG TGAGATGGCT CAGTGGGTAA GAGCACCTGA CTGCTCTTCC 
GAAGGTCCGG AGTTCAAATC CCAGCAACCA CATGGTGGCT CACAACCACC CGTAATGAGA 
TCTGACGCCC TCTTCTGGAG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Als2 ( NM_028717.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082993.4 Mus musculus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15317
Clone ID Related Accession (Same CDS sequence)	NM_028717.6 , NM_001159948.2
Accession Version	NM_028717.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	alsin isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153652.3, BC046828.1 and AB053307.1. On May 19, 2009 this sequence version replaced NM_028717.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB053307.1, BC046828.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCAA AGAAGAAAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGTGGA 
AAGACAGTTC TTCAGGCAGC CCTTGGTGTG AGGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AAAAGTGAAT CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTGAC AGAGAGCGGG GTGGTGTACA TGTGGGGAGA GAATGCTGCC 
GGGCAGTGTG CGGTAGCTAA CCAGCAGTAT GTGCCGGAGC CGAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCGTC ATTAGCAGTT AGGATTCTGC AATTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCACT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGCCTCATCA CCACCACTTT CCCAGTGACA AAGCCACAGA AGGTGGAACA CCTTGCTGGA 
CGCGTGGTGC TCCAGGTGGC CTGCGGTGCA TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCTCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCATGTGATA ATATCGGACA GCCATTGCTG CCCTTTAGGT 
GTGACATTGT CCGAGTCTCA AGCAGAAAAG CATGCCAGCC CTGCTCCCAG CCCTCACCCA 
GAGGCACTGG ATGAGCAGGG AGAGGTGTTT GAGAACACGG TGGTAGAAGC TGAACTGAAC 
ATGGGAAGCA GTCAGACCAC AAGTGGCAGT GCCATTTCCA CCCAGCAGAA CGTCGTGGGA 
ACAGCTGAAG TGTCTTCTGC CAGAACAGCT CCGTCATACC CAGACACCCA TGCGGTAACT 
GCATACCTGC AGAAGCTGTC AGAGCATTCG ATGAGGGAGA ACCATGAGCC TGGAGAAAAG 
CCACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTCAGA 
GTGGCTGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTACAGC 
ACTGCCCCCT GCGAGACGGC AGCTCAGTCG GGCAGTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCC GAGAAGAGCA GGTGAAACAG GAGTCACTGC AAGGGAAGAA AAGCTCAAGT 
CTCATGGACA TCAGAGAGGA GGAGTTGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGG 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCTG CGAGGGTGAA AACTCGGACA 
GTGGTTCTGA CTCCCACATA CAGTGGAGAA GCAGATGCCC TTCTGCCTTC CCTGAGGACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTAG GGCACGGCGA CGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTAATCCA CCTGGAGGCG 
GGCGGCTCCC ACTCCCTCGC ACTCACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTTGGTC AGCTTGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCAAAGGTT 
AGCAGTGAAA ATGGAGTCTG GAGTGTAGCT GCAGGCCAAG ATTATTCCTT GTTTTTAGTG 
GACACGGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GACAGGACCG TGCAGAAGGT 
GATACCCTGC CAGAGAATCC CAGTGGTACA AAGACTCCAG TACTTCTCTC CTGTAGTAAG 
CTTGGATACA TAAGCAGAGT AACAGCAGGA AAAGATAGCT ATCTAGCCTT GGTGGATAAG 
AACATCATGG GATACATCGC CAGTCTCCAT GAGTTGGCTT CTACAGAAAG ACGGTTTTAC 
TCAAAACTGA GCGAAATCAA ATCACAGATA CTTAGGCCTC TTCTCAGTTT AGAAAATTTG 
GGCACAGTGA CCACTGTCCA GCTGTTGCAG GAAGTTGCCA GCCGGTTCAG CAAGTTGTGT 
TACCTCATTG GGCAGCATGG AGCCTCACTA AGCAGCTACC TACAGGGCAT GAAGGAAGCC 
AGCAGCCTGG TCATCATGAA GCACTCAAGT CTTTTCCTGG ACAGCTACAC AGAGTACTGC 
ACATCAGTTT CAAATTTCCT GGTTATGGGA GGATTCCAGC TTCTTGCTAA GCCTGCCATT 
GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC 
ACTCAGTTGA TGGAAATCCT GAACATGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT 
TATGCAAAAG TTTTGCTAAA GCTTGCCACT TGCTTTGAAG TGACATCTCC AGAGTATCAA 
AAGCTGCAGG ATTCCAGTTC TTGCTATGAG TCTCTTGCTC TCCATCTTGG CAAGAAGAGG 
AAGGAAGCAG AGTACACACT GAGCTTCTGG AAGACCTTTC CTGGGAAAAT GACGGATTCC 
TTGAGGAAGC CAGAGCGCCG GCTGCTGTGT GAGAGCAGTA ACCGAGCCCT CTCCCTGCAG 
CATGCCGGCA GGTTTTCTGT GAATTGGTTC ATTCTCTTCA ATGATGCCCT GGTCCATGCT 
CAGTTCTCTA CACACCACGT GTTCCCTTTG GCCACACTCT GGGCAGAGCC ACTTTCTGAA 
GAAGCTGGTA GCGTGAATGG CTTAAAGATA ACTACACCTG AAGAACAATT CACACTCATT 
TCTTCAACAC CCCAGGAAAA GACCAAGTGG CTTCGGGCTA TCAGCCAAGC TGTGGATCAG 
GCTTTGAGGG GGACGTCCGA TTTCCCACTT TACGGAGGCG GCAGCAGTGT TCAGAGACAG 
GAACCACCCA TCTCAAGAAG TGCCAAATAC ACTTTCTACA AGGATACTCG CCTAAAGGAT 
GCCACTTACG ATGGGCGCTG GCTTTCAGGG AAGCCTCATG GCAGGGGTGT TCTGAAGTGG 
CCTGATGGAA AGATGTACTC TGGCATGTTC AGGAATGGCT TGGAAGATGG ATATGGTGAA 
TACAGAATCC CTAACAAGGC CCTGAACAAA GAAGACCATT ATGTAGGCCA TTGGAAAGAG 
GGGAAAATGT GTGGGCAAGG AGTCTACAGC TATGCCTCTG GTGAAGTGTT TGAAGGCTGC 
TTTCAAGATA ACATGCGCCA TGGGCATGGT CTGCTCCGGA GTGGAAAACT GACTTCTTCT 
TCTCCTAGCA TGTTCATTGG CCAGTGGGTA ATGGATAAGA AAGCAGGATA TGGCGTCTTT 
GATGATATCA CCAGGGGAGA AAAGTACATG GGAATGTGGC AGGATGATGT GTGCCAAGGG 
AATGGGGTAG TAGTCACCCA GTTTGGGTTA TACTACGAAG GCAACTTCCA CCTGAATAAG 
ATGATGGGAA ATGGGGTTTT GCTTTCTGAA GATGATACCA TCTATGAAGG AGAATTTTCC 
GATGACTGGA CACTTAGTGG AAAGGGAACG CTGACTATGC CACATGGAGA TTATATTGAA 
GGTTATTTTA GTGGAGAATG GGGATCTGGG ATAAAAATCA CTGGGACCTA CTTCAAACCT 
AGCCTGTATG AGAGCGATAA GGACAAGCCC AAAGCCTTCA GGAAGCTGGG GAACCTGGCC 
GTGGCAGCAG ACGAGAAATG GAGAGCAGTG TTTGAAGAAT GCTGGCGCCA GCTGGGCTGT 
GAGAGCCCAG GCCAAGGGGA GGTTTGGAAA GCATGGGATA ATATTGCTGT GGCCTTGACC 
ACGAACCGTC GCCAGCATAA AGACAGTCCA GAAATACTAA GCCGCTCTCA GACTCAGACC 
CTGGAGAGTT TGGAGTACAT TCCCCAGCAC ATTGGCGCCT TCTCTGTGGA GAAATATGAT 
GACATCAAGA AGTATTTAAT AAAGGCCTGT GATACTCCTC TGCACCCACT GGGCAGGCTT 
GTGGAGACCC TGGTTGCGGT GTATAGAATG ACATATGTGG GTGTAGGGGC CAACCGCCGG 
TTACTGCAGG AAGCTGTGAA GGAGATTAAA TCTTATCTCA AGAGGATTTT CCAGCTTGTG 
AGGTTCTTGT TTCCTGAGCT TCCTGAGGAG GGCAGCACAA TTCCTCTTTC TGCTCCTCTG 
CCCACTGGAA GGAGATCCTT CTGTACTGGA AAATCGGATT CCAGATCCGA GTCACCAGAA 
CCAGGTTATG TAGTAACAAG TTCTGGCTTA CTGCTTCCGG TGCTGCTGCC ACGGCTCTAC 
CCACCTCTCT TCATGCTCTA TGCCCTGGAT AATGACCGAG AGGAAGACAT TTACTGGGAA 
TGTGTGCTTC GACTAAACAA GCAGCCAGAT ATTGCTCTCT TGGGCTTCCT TGGAGTACAG 
AAAAAATTCT GGCCAGCCAC CTTGTCAATC CTTGGAGAGA GTAAAAAGGT GTTGTCAACC 
ACAAAGGATG CTTGCTTTGC ATCTGCAGTA GAATGCCTGC AGCAGATCAG CACAACATTT 
ACTCCATCAG ACAAGCTTAA AGTGATCCAG CAGACCTTTG AAGAGATCTC CCAGAGTGTC 
CTTGCATCGC TGCAGGAGGA CTTCCTCTGG TCCATGGATG ACTTGTTCCC CGTCTTCTTA 
TACGTGGTGC TGCGGGCCAG GATTCGGAAC TTGGGCTCTG AAGTTCACCT CATTGAGGAT 
CTGATGGACC CCTTTCTCCA GCATGGGGAA CAAGGCATCA TGTTCACCAC CTTGAAGGCC 
TGTTACTTCC AGATTCAGCG GGAGAAGCTT AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082993.4
CDS	136..5091
Translation	MDSKKKSSTEAEGSKERGLVHVWQAGSFSLTPERLPGWGGKTVLQAALGVRHGVLLTEDGEVYSFGTLPWKSESAEICPSSPLLESALVGHHVITVATGSFHSGAVTESGVVYMWGENAAGQCAVANQQYVPEPSPVSISDSETSPSLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPPQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEKHASPAPSPHPEALDEQGEVFENTVVEAELNMGSSQTTSGSAISTQQNVVGTAEVSSARTAPSYPDTHAVTAYLQKLSEHSMRENHEPGEKPPQVQPLVEEAVPDLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCETAAQSGSASTGPESLKDLREEQVKQESLQGKKSSSLMDIREEELEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGSHSLALTAKSQVYSWGSNTFGQLGHSEFPTTVPRLSKVSSENGVWSVAAGQDYSLFLVDTEDFQPGLYYSGRQDRAEGDTLPENPSGTKTPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELASTERRFYSKLSEIKSQILRPLLSLENLGTVTTVQLLQEVASRFSKLCYLIGQHGASLSSYLQGMKEASSLVIMKHSSLFLDSYTEYCTSVSNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNMLFFLPIRRLHNYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGKKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGSVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGTSDFPLYGGGSSVQRQEPPISRSAKYTFYKDTRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPHGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDKPKAFRKLGNLAVAADEKWRAVFEECWRQLGCESPGQGEVWKAWDNIAVALTTNRRQHKDSPEILSRSQTQTLESLEYIPQHIGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTGRRSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDREEDIYWECVLRLNKQPDIALLGFLGVQKKFWPATLSILGESKKVLSTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPFLQHGEQGIMFTTLKACYFQIQREKLN

Target ORF information:

RefSeq Version	NM_028717.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028717.6

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGTGGA 
AAGACAGTTC TTCAGGCAGC CCTTGGTGTG AGGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AAAAGTGAAT CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTGAC AGAGAGCGGG GTGGTGTACA TGTGGGGAGA GAATGCTGCC 
GGGCAGTGTG CGGTAGCTAA CCAGCAGTAT GTGCCGGAGC CGAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCGTC ATTAGCAGTT AGGATTCTGC AATTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCACT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGCCTCATCA CCACCACTTT CCCAGTGACA AAGCCACAGA AGGTGGAACA CCTTGCTGGA 
CGCGTGGTGC TCCAGGTGGC CTGCGGTGCA TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCTCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCATGTGATA ATATCGGACA GCCATTGCTG CCCTTTAGGT 
GTGACATTGT CCGAGTCTCA AGCAGAAAAG CATGCCAGCC CTGCTCCCAG CCCTCACCCA 
GAGGCACTGG ATGAGCAGGG AGAGGTGTTT GAGAACACGG TGGTAGAAGC TGAACTGAAC 
ATGGGAAGCA GTCAGACCAC AAGTGGCAGT GCCATTTCCA CCCAGCAGAA CGTCGTGGGA 
ACAGCTGAAG TGTCTTCTGC CAGAACAGCT CCGTCATACC CAGACACCCA TGCGGTAACT 
GCATACCTGC AGAAGCTGTC AGAGCATTCG ATGAGGGAGA ACCATGAGCC TGGAGAAAAG 
CCACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTCAGA 
GTGGCTGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTACAGC 
ACTGCCCCCT GCGAGACGGC AGCTCAGTCG GGCAGTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCC GAGAAGAGCA GGTGAAACAG GAGTCACTGC AAGGGAAGAA AAGCTCAAGT 
CTCATGGACA TCAGAGAGGA GGAGTTGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGG 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCTG CGAGGGTGAA AACTCGGACA 
GTGGTTCTGA CTCCCACATA CAGTGGAGAA GCAGATGCCC TTCTGCCTTC CCTGAGGACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTAG GGCACGGCGA CGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTAATCCA CCTGGAGGCG 
GGCGGCTCCC ACTCCCTCGC ACTCACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTTGGTC AGCTTGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCAAAGGTT 
AGCAGTGAAA ATGGAGTCTG GAGTGTAGCT GCAGGCCAAG ATTATTCCTT GTTTTTAGTG 
GACACGGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GACAGGACCG TGCAGAAGGT 
GATACCCTGC CAGAGAATCC CAGTGGTACA AAGACTCCAG TACTTCTCTC CTGTAGTAAG 
CTTGGATACA TAAGCAGAGT AACAGCAGGA AAAGATAGCT ATCTAGCCTT GGTGGATAAG 
AACATCATGG GATACATCGC CAGTCTCCAT GAGTTGGCTT CTACAGAAAG ACGGTTTTAC 
TCAAAACTGA GCGAAATCAA ATCACAGATA CTTAGGCCTC TTCTCAGTTT AGAAAATTTG 
GGCACAGTGA CCACTGTCCA GCTGTTGCAG GAAGTTGCCA GCCGGTTCAG CAAGTTGTGT 
TACCTCATTG GGCAGCATGG AGCCTCACTA AGCAGCTACC TACAGGGCAT GAAGGAAGCC 
AGCAGCCTGG TCATCATGAA GCACTCAAGT CTTTTCCTGG ACAGCTACAC AGAGTACTGC 
ACATCAGTTT CAAATTTCCT GGTTATGGGA GGATTCCAGC TTCTTGCTAA GCCTGCCATT 
GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC 
ACTCAGTTGA TGGAAATCCT GAACATGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT 
TATGCAAAAG TTTTGCTAAA GCTTGCCACT TGCTTTGAAG TGACATCTCC AGAGTATCAA 
AAGCTGCAGG ATTCCAGTTC TTGCTATGAG TCTCTTGCTC TCCATCTTGG CAAGAAGAGG 
AAGGAAGCAG AGTACACACT GAGCTTCTGG AAGACCTTTC CTGGGAAAAT GACGGATTCC 
TTGAGGAAGC CAGAGCGCCG GCTGCTGTGT GAGAGCAGTA ACCGAGCCCT CTCCCTGCAG 
CATGCCGGCA GGTTTTCTGT GAATTGGTTC ATTCTCTTCA ATGATGCCCT GGTCCATGCT 
CAGTTCTCTA CACACCACGT GTTCCCTTTG GCCACACTCT GGGCAGAGCC ACTTTCTGAA 
GAAGCTGGTA GCGTGAATGG CTTAAAGATA ACTACACCTG AAGAACAATT CACACTCATT 
TCTTCAACAC CCCAGGAAAA GACCAAGTGG CTTCGGGCTA TCAGCCAAGC TGTGGATCAG 
GCTTTGAGGG GGACGTCCGA TTTCCCACTT TACGGAGGCG GCAGCAGTGT TCAGAGACAG 
GAACCACCCA TCTCAAGAAG TGCCAAATAC ACTTTCTACA AGGATACTCG CCTAAAGGAT 
GCCACTTACG ATGGGCGCTG GCTTTCAGGG AAGCCTCATG GCAGGGGTGT TCTGAAGTGG 
CCTGATGGAA AGATGTACTC TGGCATGTTC AGGAATGGCT TGGAAGATGG ATATGGTGAA 
TACAGAATCC CTAACAAGGC CCTGAACAAA GAAGACCATT ATGTAGGCCA TTGGAAAGAG 
GGGAAAATGT GTGGGCAAGG AGTCTACAGC TATGCCTCTG GTGAAGTGTT TGAAGGCTGC 
TTTCAAGATA ACATGCGCCA TGGGCATGGT CTGCTCCGGA GTGGAAAACT GACTTCTTCT 
TCTCCTAGCA TGTTCATTGG CCAGTGGGTA ATGGATAAGA AAGCAGGATA TGGCGTCTTT 
GATGATATCA CCAGGGGAGA AAAGTACATG GGAATGTGGC AGGATGATGT GTGCCAAGGG 
AATGGGGTAG TAGTCACCCA GTTTGGGTTA TACTACGAAG GCAACTTCCA CCTGAATAAG 
ATGATGGGAA ATGGGGTTTT GCTTTCTGAA GATGATACCA TCTATGAAGG AGAATTTTCC 
GATGACTGGA CACTTAGTGG AAAGGGAACG CTGACTATGC CACATGGAGA TTATATTGAA 
GGTTATTTTA GTGGAGAATG GGGATCTGGG ATAAAAATCA CTGGGACCTA CTTCAAACCT 
AGCCTGTATG AGAGCGATAA GGACAAGCCC AAAGCCTTCA GGAAGCTGGG GAACCTGGCC 
GTGGCAGCAG ACGAGAAATG GAGAGCAGTG TTTGAAGAAT GCTGGCGCCA GCTGGGCTGT 
GAGAGCCCAG GCCAAGGGGA GGTTTGGAAA GCATGGGATA ATATTGCTGT GGCCTTGACC 
ACGAACCGTC GCCAGCATAA AGACAGTCCA GAAATACTAA GCCGCTCTCA GACTCAGACC 
CTGGAGAGTT TGGAGTACAT TCCCCAGCAC ATTGGCGCCT TCTCTGTGGA GAAATATGAT 
GACATCAAGA AGTATTTAAT AAAGGCCTGT GATACTCCTC TGCACCCACT GGGCAGGCTT 
GTGGAGACCC TGGTTGCGGT GTATAGAATG ACATATGTGG GTGTAGGGGC CAACCGCCGG 
TTACTGCAGG AAGCTGTGAA GGAGATTAAA TCTTATCTCA AGAGGATTTT CCAGCTTGTG 
AGGTTCTTGT TTCCTGAGCT TCCTGAGGAG GGCAGCACAA TTCCTCTTTC TGCTCCTCTG 
CCCACTGGAA GGAGATCCTT CTGTACTGGA AAATCGGATT CCAGATCCGA GTCACCAGAA 
CCAGGTTATG TAGTAACAAG TTCTGGCTTA CTGCTTCCGG TGCTGCTGCC ACGGCTCTAC 
CCACCTCTCT TCATGCTCTA TGCCCTGGAT AATGACCGAG AGGAAGACAT TTACTGGGAA 
TGTGTGCTTC GACTAAACAA GCAGCCAGAT ATTGCTCTCT TGGGCTTCCT TGGAGTACAG 
AAAAAATTCT GGCCAGCCAC CTTGTCAATC CTTGGAGAGA GTAAAAAGGT GTTGTCAACC 
ACAAAGGATG CTTGCTTTGC ATCTGCAGTA GAATGCCTGC AGCAGATCAG CACAACATTT 
ACTCCATCAG ACAAGCTTAA AGTGATCCAG CAGACCTTTG AAGAGATCTC CCAGAGTGTC 
CTTGCATCGC TGCAGGAGGA CTTCCTCTGG TCCATGGATG ACTTGTTCCC CGTCTTCTTA 
TACGTGGTGC TGCGGGCCAG GATTCGGAAC TTGGGCTCTG AAGTTCACCT CATTGAGGAT 
CTGATGGACC CCTTTCTCCA GCATGGGGAA CAAGGCATCA TGTTCACCAC CTTGAAGGCC 
TGTTACTTCC AGATTCAGCG GGAGAAGCTT AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Als2 ( NM_001159948.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153420.2 Mus musculus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant 3, mRNA.

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CloneID	OMu15317
Clone ID Related Accession (Same CDS sequence)	NM_028717.6 , NM_001159948.2
Accession Version	NM_001159948.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	alsin isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK029858.1, BC046828.1, AB053307.1 and DV648961.1. On May 19, 2009 this sequence version replaced NM_001159948.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 3 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK029858.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCAA AGAAGAAAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGTGGA 
AAGACAGTTC TTCAGGCAGC CCTTGGTGTG AGGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AAAAGTGAAT CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTGAC AGAGAGCGGG GTGGTGTACA TGTGGGGAGA GAATGCTGCC 
GGGCAGTGTG CGGTAGCTAA CCAGCAGTAT GTGCCGGAGC CGAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCGTC ATTAGCAGTT AGGATTCTGC AATTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCACT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGCCTCATCA CCACCACTTT CCCAGTGACA AAGCCACAGA AGGTGGAACA CCTTGCTGGA 
CGCGTGGTGC TCCAGGTGGC CTGCGGTGCA TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCTCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCATGTGATA ATATCGGACA GCCATTGCTG CCCTTTAGGT 
GTGACATTGT CCGAGTCTCA AGCAGAAAAG CATGCCAGCC CTGCTCCCAG CCCTCACCCA 
GAGGCACTGG ATGAGCAGGG AGAGGTGTTT GAGAACACGG TGGTAGAAGC TGAACTGAAC 
ATGGGAAGCA GTCAGACCAC AAGTGGCAGT GCCATTTCCA CCCAGCAGAA CGTCGTGGGA 
ACAGCTGAAG TGTCTTCTGC CAGAACAGCT CCGTCATACC CAGACACCCA TGCGGTAACT 
GCATACCTGC AGAAGCTGTC AGAGCATTCG ATGAGGGAGA ACCATGAGCC TGGAGAAAAG 
CCACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTCAGA 
GTGGCTGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTACAGC 
ACTGCCCCCT GCGAGACGGC AGCTCAGTCG GGCAGTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCC GAGAAGAGCA GGTGAAACAG GAGTCACTGC AAGGGAAGAA AAGCTCAAGT 
CTCATGGACA TCAGAGAGGA GGAGTTGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGG 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCTG CGAGGGTGAA AACTCGGACA 
GTGGTTCTGA CTCCCACATA CAGTGGAGAA GCAGATGCCC TTCTGCCTTC CCTGAGGACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTAG GGCACGGCGA CGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTAATCCA CCTGGAGGCG 
GGCGGCTCCC ACTCCCTCGC ACTCACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTTGGTC AGCTTGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCAAAGGTT 
AGCAGTGAAA ATGGAGTCTG GAGTGTAGCT GCAGGCCAAG ATTATTCCTT GTTTTTAGTG 
GACACGGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GACAGGACCG TGCAGAAGGT 
GATACCCTGC CAGAGAATCC CAGTGGTACA AAGACTCCAG TACTTCTCTC CTGTAGTAAG 
CTTGGATACA TAAGCAGAGT AACAGCAGGA AAAGATAGCT ATCTAGCCTT GGTGGATAAG 
AACATCATGG GATACATCGC CAGTCTCCAT GAGTTGGCTT CTACAGAAAG ACGGTTTTAC 
TCAAAACTGA GCGAAATCAA ATCACAGATA CTTAGGCCTC TTCTCAGTTT AGAAAATTTG 
GGCACAGTGA CCACTGTCCA GCTGTTGCAG GAAGTTGCCA GCCGGTTCAG CAAGTTGTGT 
TACCTCATTG GGCAGCATGG AGCCTCACTA AGCAGCTACC TACAGGGCAT GAAGGAAGCC 
AGCAGCCTGG TCATCATGAA GCACTCAAGT CTTTTCCTGG ACAGCTACAC AGAGTACTGC 
ACATCAGTTT CAAATTTCCT GGTTATGGGA GGATTCCAGC TTCTTGCTAA GCCTGCCATT 
GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC 
ACTCAGTTGA TGGAAATCCT GAACATGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT 
TATGCAAAAG TTTTGCTAAA GCTTGCCACT TGCTTTGAAG TGACATCTCC AGAGTATCAA 
AAGCTGCAGG ATTCCAGTTC TTGCTATGAG TCTCTTGCTC TCCATCTTGG CAAGAAGAGG 
AAGGAAGCAG AGTACACACT GAGCTTCTGG AAGACCTTTC CTGGGAAAAT GACGGATTCC 
TTGAGGAAGC CAGAGCGCCG GCTGCTGTGT GAGAGCAGTA ACCGAGCCCT CTCCCTGCAG 
CATGCCGGCA GGTTTTCTGT GAATTGGTTC ATTCTCTTCA ATGATGCCCT GGTCCATGCT 
CAGTTCTCTA CACACCACGT GTTCCCTTTG GCCACACTCT GGGCAGAGCC ACTTTCTGAA 
GAAGCTGGTA GCGTGAATGG CTTAAAGATA ACTACACCTG AAGAACAATT CACACTCATT 
TCTTCAACAC CCCAGGAAAA GACCAAGTGG CTTCGGGCTA TCAGCCAAGC TGTGGATCAG 
GCTTTGAGGG GGACGTCCGA TTTCCCACTT TACGGAGGCG GCAGCAGTGT TCAGAGACAG 
GAACCACCCA TCTCAAGAAG TGCCAAATAC ACTTTCTACA AGGATACTCG CCTAAAGGAT 
GCCACTTACG ATGGGCGCTG GCTTTCAGGG AAGCCTCATG GCAGGGGTGT TCTGAAGTGG 
CCTGATGGAA AGATGTACTC TGGCATGTTC AGGAATGGCT TGGAAGATGG ATATGGTGAA 
TACAGAATCC CTAACAAGGC CCTGAACAAA GAAGACCATT ATGTAGGCCA TTGGAAAGAG 
GGGAAAATGT GTGGGCAAGG AGTCTACAGC TATGCCTCTG GTGAAGTGTT TGAAGGCTGC 
TTTCAAGATA ACATGCGCCA TGGGCATGGT CTGCTCCGGA GTGGAAAACT GACTTCTTCT 
TCTCCTAGCA TGTTCATTGG CCAGTGGGTA ATGGATAAGA AAGCAGGATA TGGCGTCTTT 
GATGATATCA CCAGGGGAGA AAAGTACATG GGAATGTGGC AGGATGATGT GTGCCAAGGG 
AATGGGGTAG TAGTCACCCA GTTTGGGTTA TACTACGAAG GCAACTTCCA CCTGAATAAG 
ATGATGGGAA ATGGGGTTTT GCTTTCTGAA GATGATACCA TCTATGAAGG AGAATTTTCC 
GATGACTGGA CACTTAGTGG AAAGGGAACG CTGACTATGC CACATGGAGA TTATATTGAA 
GGTTATTTTA GTGGAGAATG GGGATCTGGG ATAAAAATCA CTGGGACCTA CTTCAAACCT 
AGCCTGTATG AGAGCGATAA GGACAAGCCC AAAGCCTTCA GGAAGCTGGG GAACCTGGCC 
GTGGCAGCAG ACGAGAAATG GAGAGCAGTG TTTGAAGAAT GCTGGCGCCA GCTGGGCTGT 
GAGAGCCCAG GCCAAGGGGA GGTTTGGAAA GCATGGGATA ATATTGCTGT GGCCTTGACC 
ACGAACCGTC GCCAGCATAA AGACAGTCCA GAAATACTAA GCCGCTCTCA GACTCAGACC 
CTGGAGAGTT TGGAGTACAT TCCCCAGCAC ATTGGCGCCT TCTCTGTGGA GAAATATGAT 
GACATCAAGA AGTATTTAAT AAAGGCCTGT GATACTCCTC TGCACCCACT GGGCAGGCTT 
GTGGAGACCC TGGTTGCGGT GTATAGAATG ACATATGTGG GTGTAGGGGC CAACCGCCGG 
TTACTGCAGG AAGCTGTGAA GGAGATTAAA TCTTATCTCA AGAGGATTTT CCAGCTTGTG 
AGGTTCTTGT TTCCTGAGCT TCCTGAGGAG GGCAGCACAA TTCCTCTTTC TGCTCCTCTG 
CCCACTGGAA GGAGATCCTT CTGTACTGGA AAATCGGATT CCAGATCCGA GTCACCAGAA 
CCAGGTTATG TAGTAACAAG TTCTGGCTTA CTGCTTCCGG TGCTGCTGCC ACGGCTCTAC 
CCACCTCTCT TCATGCTCTA TGCCCTGGAT AATGACCGAG AGGAAGACAT TTACTGGGAA 
TGTGTGCTTC GACTAAACAA GCAGCCAGAT ATTGCTCTCT TGGGCTTCCT TGGAGTACAG 
AAAAAATTCT GGCCAGCCAC CTTGTCAATC CTTGGAGAGA GTAAAAAGGT GTTGTCAACC 
ACAAAGGATG CTTGCTTTGC ATCTGCAGTA GAATGCCTGC AGCAGATCAG CACAACATTT 
ACTCCATCAG ACAAGCTTAA AGTGATCCAG CAGACCTTTG AAGAGATCTC CCAGAGTGTC 
CTTGCATCGC TGCAGGAGGA CTTCCTCTGG TCCATGGATG ACTTGTTCCC CGTCTTCTTA 
TACGTGGTGC TGCGGGCCAG GATTCGGAAC TTGGGCTCTG AAGTTCACCT CATTGAGGAT 
CTGATGGACC CCTTTCTCCA GCATGGGGAA CAAGGCATCA TGTTCACCAC CTTGAAGGCC 
TGTTACTTCC AGATTCAGCG GGAGAAGCTT AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153420.2
CDS	305..5260
Translation	MDSKKKSSTEAEGSKERGLVHVWQAGSFSLTPERLPGWGGKTVLQAALGVRHGVLLTEDGEVYSFGTLPWKSESAEICPSSPLLESALVGHHVITVATGSFHSGAVTESGVVYMWGENAAGQCAVANQQYVPEPSPVSISDSETSPSLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPPQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEKHASPAPSPHPEALDEQGEVFENTVVEAELNMGSSQTTSGSAISTQQNVVGTAEVSSARTAPSYPDTHAVTAYLQKLSEHSMRENHEPGEKPPQVQPLVEEAVPDLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCETAAQSGSASTGPESLKDLREEQVKQESLQGKKSSSLMDIREEELEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGSHSLALTAKSQVYSWGSNTFGQLGHSEFPTTVPRLSKVSSENGVWSVAAGQDYSLFLVDTEDFQPGLYYSGRQDRAEGDTLPENPSGTKTPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELASTERRFYSKLSEIKSQILRPLLSLENLGTVTTVQLLQEVASRFSKLCYLIGQHGASLSSYLQGMKEASSLVIMKHSSLFLDSYTEYCTSVSNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNMLFFLPIRRLHNYAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGKKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGSVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGTSDFPLYGGGSSVQRQEPPISRSAKYTFYKDTRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDVCQGNGVVVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPHGDYIEGYFSGEWGSGIKITGTYFKPSLYESDKDKPKAFRKLGNLAVAADEKWRAVFEECWRQLGCESPGQGEVWKAWDNIAVALTTNRRQHKDSPEILSRSQTQTLESLEYIPQHIGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTIPLSAPLPTGRRSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDREEDIYWECVLRLNKQPDIALLGFLGVQKKFWPATLSILGESKKVLSTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPFLQHGEQGIMFTTLKACYFQIQREKLN

Target ORF information:

RefSeq Version	NM_001159948.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus alsin Rho guanine nucleotide exchange factor (Als2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159948.2

ORF Insert Sequence:

ATGGACTCAA AGAAGAAAAG CTCAACAGAG GCAGAAGGAT CCAAAGAAAG AGGCCTAGTC 
CATGTCTGGC AGGCAGGATC CTTTTCTCTA ACACCAGAGA GGTTGCCAGG CTGGGGTGGA 
AAGACAGTTC TTCAGGCAGC CCTTGGTGTG AGGCATGGAG TTCTTCTGAC TGAAGATGGT 
GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AAAAGTGAAT CAGCAGAAAT TTGTCCAAGC 
AGCCCCCTTC TAGAAAGTGC CCTGGTTGGG CATCATGTTA TTACTGTGGC AACAGGGAGC 
TTCCACAGTG GAGCAGTGAC AGAGAGCGGG GTGGTGTACA TGTGGGGAGA GAATGCTGCC 
GGGCAGTGTG CGGTAGCTAA CCAGCAGTAT GTGCCGGAGC CGAGTCCTGT CAGCATTTCT 
GACTCGGAGA CCAGCCCGTC ATTAGCAGTT AGGATTCTGC AATTGGCATG TGGCGAGGAA 
CACACACTGG CATTGTCACT CAGCAGAGAG ATCTGGGCAT GGGGCACCGG CTGTCAGCTG 
GGCCTCATCA CCACCACTTT CCCAGTGACA AAGCCACAGA AGGTGGAACA CCTTGCTGGA 
CGCGTGGTGC TCCAGGTGGC CTGCGGTGCA TTCCACAGCC TTGCACTTGT GCAGTGCCTC 
CCTCCTCAGG ATCTGAAGCC AGTCCCAGAG AGATGCAATC AGTGCAGCCA GCTGCTCATC 
ACCATGACAG ACAAAGAGGA CCATGTGATA ATATCGGACA GCCATTGCTG CCCTTTAGGT 
GTGACATTGT CCGAGTCTCA AGCAGAAAAG CATGCCAGCC CTGCTCCCAG CCCTCACCCA 
GAGGCACTGG ATGAGCAGGG AGAGGTGTTT GAGAACACGG TGGTAGAAGC TGAACTGAAC 
ATGGGAAGCA GTCAGACCAC AAGTGGCAGT GCCATTTCCA CCCAGCAGAA CGTCGTGGGA 
ACAGCTGAAG TGTCTTCTGC CAGAACAGCT CCGTCATACC CAGACACCCA TGCGGTAACT 
GCATACCTGC AGAAGCTGTC AGAGCATTCG ATGAGGGAGA ACCATGAGCC TGGAGAAAAG 
CCACCCCAGG TCCAGCCTCT TGTAGAAGAA GCAGTTCCTG ATCTTCACAG TCCACCAACC 
ACAAGCACCT CAGCCCTCAA CAGCTTGGTG GTCTCCTGTG CATCTGCTGT TGGTGTCAGA 
GTGGCTGCCA CCTATGAAGC TGGGGCCTTG TCTCTCAAGA AAGTTATGAA CTTTTACAGC 
ACTGCCCCCT GCGAGACGGC AGCTCAGTCG GGCAGTGCCT CCACAGGCCC AGAAAGTCTG 
AAAGATCTCC GAGAAGAGCA GGTGAAACAG GAGTCACTGC AAGGGAAGAA AAGCTCAAGT 
CTCATGGACA TCAGAGAGGA GGAGTTGGAG GGAGGGAGTC GAAGACTCTC CCTCCCAGGG 
TTGTTGTCGC AAGTTTCCCC CAGGCTCTTA AGGAAGGCTG CGAGGGTGAA AACTCGGACA 
GTGGTTCTGA CTCCCACATA CAGTGGAGAA GCAGATGCCC TTCTGCCTTC CCTGAGGACA 
GAGGTGTGGA CCTGGGGGAA AGGCAAGGAA GGGCAGCTAG GGCACGGCGA CGTCCTGCCC 
AGGCTTCAGC CGTTGTGTGT CAAGTGTCTG GATGGTAAAG AGGTAATCCA CCTGGAGGCG 
GGCGGCTCCC ACTCCCTCGC ACTCACTGCG AAATCTCAGG TTTACTCATG GGGCAGTAAT 
ACCTTTGGTC AGCTTGGGCA TTCTGAGTTT CCAACAACGG TTCCTCGACT CTCAAAGGTT 
AGCAGTGAAA ATGGAGTCTG GAGTGTAGCT GCAGGCCAAG ATTATTCCTT GTTTTTAGTG 
GACACGGAAG ACTTCCAGCC TGGGTTGTAT TACAGTGGCC GACAGGACCG TGCAGAAGGT 
GATACCCTGC CAGAGAATCC CAGTGGTACA AAGACTCCAG TACTTCTCTC CTGTAGTAAG 
CTTGGATACA TAAGCAGAGT AACAGCAGGA AAAGATAGCT ATCTAGCCTT GGTGGATAAG 
AACATCATGG GATACATCGC CAGTCTCCAT GAGTTGGCTT CTACAGAAAG ACGGTTTTAC 
TCAAAACTGA GCGAAATCAA ATCACAGATA CTTAGGCCTC TTCTCAGTTT AGAAAATTTG 
GGCACAGTGA CCACTGTCCA GCTGTTGCAG GAAGTTGCCA GCCGGTTCAG CAAGTTGTGT 
TACCTCATTG GGCAGCATGG AGCCTCACTA AGCAGCTACC TACAGGGCAT GAAGGAAGCC 
AGCAGCCTGG TCATCATGAA GCACTCAAGT CTTTTCCTGG ACAGCTACAC AGAGTACTGC 
ACATCAGTTT CAAATTTCCT GGTTATGGGA GGATTCCAGC TTCTTGCTAA GCCTGCCATT 
GATTTCCTAA ATAAAAACCA AGAACTCTTG CAAGATTTGT CAGAAGTGAA TGATGAGAAC 
ACTCAGTTGA TGGAAATCCT GAACATGCTG TTTTTCTTGC CAATCAGACG ACTTCATAAT 
TATGCAAAAG TTTTGCTAAA GCTTGCCACT TGCTTTGAAG TGACATCTCC AGAGTATCAA 
AAGCTGCAGG ATTCCAGTTC TTGCTATGAG TCTCTTGCTC TCCATCTTGG CAAGAAGAGG 
AAGGAAGCAG AGTACACACT GAGCTTCTGG AAGACCTTTC CTGGGAAAAT GACGGATTCC 
TTGAGGAAGC CAGAGCGCCG GCTGCTGTGT GAGAGCAGTA ACCGAGCCCT CTCCCTGCAG 
CATGCCGGCA GGTTTTCTGT GAATTGGTTC ATTCTCTTCA ATGATGCCCT GGTCCATGCT 
CAGTTCTCTA CACACCACGT GTTCCCTTTG GCCACACTCT GGGCAGAGCC ACTTTCTGAA 
GAAGCTGGTA GCGTGAATGG CTTAAAGATA ACTACACCTG AAGAACAATT CACACTCATT 
TCTTCAACAC CCCAGGAAAA GACCAAGTGG CTTCGGGCTA TCAGCCAAGC TGTGGATCAG 
GCTTTGAGGG GGACGTCCGA TTTCCCACTT TACGGAGGCG GCAGCAGTGT TCAGAGACAG 
GAACCACCCA TCTCAAGAAG TGCCAAATAC ACTTTCTACA AGGATACTCG CCTAAAGGAT 
GCCACTTACG ATGGGCGCTG GCTTTCAGGG AAGCCTCATG GCAGGGGTGT TCTGAAGTGG 
CCTGATGGAA AGATGTACTC TGGCATGTTC AGGAATGGCT TGGAAGATGG ATATGGTGAA 
TACAGAATCC CTAACAAGGC CCTGAACAAA GAAGACCATT ATGTAGGCCA TTGGAAAGAG 
GGGAAAATGT GTGGGCAAGG AGTCTACAGC TATGCCTCTG GTGAAGTGTT TGAAGGCTGC 
TTTCAAGATA ACATGCGCCA TGGGCATGGT CTGCTCCGGA GTGGAAAACT GACTTCTTCT 
TCTCCTAGCA TGTTCATTGG CCAGTGGGTA ATGGATAAGA AAGCAGGATA TGGCGTCTTT 
GATGATATCA CCAGGGGAGA AAAGTACATG GGAATGTGGC AGGATGATGT GTGCCAAGGG 
AATGGGGTAG TAGTCACCCA GTTTGGGTTA TACTACGAAG GCAACTTCCA CCTGAATAAG 
ATGATGGGAA ATGGGGTTTT GCTTTCTGAA GATGATACCA TCTATGAAGG AGAATTTTCC 
GATGACTGGA CACTTAGTGG AAAGGGAACG CTGACTATGC CACATGGAGA TTATATTGAA 
GGTTATTTTA GTGGAGAATG GGGATCTGGG ATAAAAATCA CTGGGACCTA CTTCAAACCT 
AGCCTGTATG AGAGCGATAA GGACAAGCCC AAAGCCTTCA GGAAGCTGGG GAACCTGGCC 
GTGGCAGCAG ACGAGAAATG GAGAGCAGTG TTTGAAGAAT GCTGGCGCCA GCTGGGCTGT 
GAGAGCCCAG GCCAAGGGGA GGTTTGGAAA GCATGGGATA ATATTGCTGT GGCCTTGACC 
ACGAACCGTC GCCAGCATAA AGACAGTCCA GAAATACTAA GCCGCTCTCA GACTCAGACC 
CTGGAGAGTT TGGAGTACAT TCCCCAGCAC ATTGGCGCCT TCTCTGTGGA GAAATATGAT 
GACATCAAGA AGTATTTAAT AAAGGCCTGT GATACTCCTC TGCACCCACT GGGCAGGCTT 
GTGGAGACCC TGGTTGCGGT GTATAGAATG ACATATGTGG GTGTAGGGGC CAACCGCCGG 
TTACTGCAGG AAGCTGTGAA GGAGATTAAA TCTTATCTCA AGAGGATTTT CCAGCTTGTG 
AGGTTCTTGT TTCCTGAGCT TCCTGAGGAG GGCAGCACAA TTCCTCTTTC TGCTCCTCTG 
CCCACTGGAA GGAGATCCTT CTGTACTGGA AAATCGGATT CCAGATCCGA GTCACCAGAA 
CCAGGTTATG TAGTAACAAG TTCTGGCTTA CTGCTTCCGG TGCTGCTGCC ACGGCTCTAC 
CCACCTCTCT TCATGCTCTA TGCCCTGGAT AATGACCGAG AGGAAGACAT TTACTGGGAA 
TGTGTGCTTC GACTAAACAA GCAGCCAGAT ATTGCTCTCT TGGGCTTCCT TGGAGTACAG 
AAAAAATTCT GGCCAGCCAC CTTGTCAATC CTTGGAGAGA GTAAAAAGGT GTTGTCAACC 
ACAAAGGATG CTTGCTTTGC ATCTGCAGTA GAATGCCTGC AGCAGATCAG CACAACATTT 
ACTCCATCAG ACAAGCTTAA AGTGATCCAG CAGACCTTTG AAGAGATCTC CCAGAGTGTC 
CTTGCATCGC TGCAGGAGGA CTTCCTCTGG TCCATGGATG ACTTGTTCCC CGTCTTCTTA 
TACGTGGTGC TGCGGGCCAG GATTCGGAAC TTGGGCTCTG AAGTTCACCT CATTGAGGAT 
CTGATGGACC CCTTTCTCCA GCATGGGGAA CAAGGCATCA TGTTCACCAC CTTGAAGGCC 
TGTTACTTCC AGATTCAGCG GGAGAAGCTT AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional links between SQSTM1 and ALS2 in the pathogenesis of ALS: cumulative impact on the protection against mutant SOD1-mediated motor dysfunction in mice.
Human molecular genetics25(15)3321-3340(2016 08)
Hadano S,Mitsui S,Pan L,Otomo A,Kubo M,Sato K,Ono S,Onodera W,Abe K,Chen X,Koike M,Uchiyama Y,Aoki M,Warabi E,Yamamoto M,Ishii T,Yanagawa T,Shang HF,Yoshii F

Absence of alsin function leads to corticospinal motor neuron vulnerability via novel disease mechanisms.
Human molecular genetics25(6)1074-87(2016 Mar)
Gautam M,Jara JH,Sekerkova G,Yasvoina MV,Martina M,Özdinler PH

Are alsin and spartin novel interaction partners?
Biochemical and biophysical research communications427(1)1-4(2012 Oct)
Çobanoğlu G,Ozansoy M,Başak AN

Different human copper-zinc superoxide dismutase mutants, SOD1G93A and SOD1H46R, exert distinct harmful effects on gross phenotype in mice.
PloS one7(3)e33409(2012)
Pan L,Yoshii Y,Otomo A,Ogawa H,Iwasaki Y,Shang HF,Hadano S

Alsin and SOD1(G93A) proteins regulate endosomal reactive oxygen species production by glial cells and proinflammatory pathways responsible for neurotoxicity.
The Journal of biological chemistry286(46)40151-62(2011 Nov)
Li Q,Spencer NY,Pantazis NJ,Engelhardt JF

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Als2 cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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