als2b gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005165740.3	XP_005165797.1	alsin isoform X3
XM_009302177.2	XP_009300452.1	alsin isoform X4
XM_009302175.3	XP_009300450.1	alsin isoform X1
XM_009302177.3	XP_009300452.1	alsin isoform X4
XM_005165740.4	XP_005165797.1	alsin isoform X3
XM_002662296.5	XP_002662342.2	alsin isoform X5
XM_002662296.6	XP_002662342.2	alsin isoform X5
XM_009302176.2	XP_009300451.1	alsin isoform X2
XM_009302175.2	XP_009300450.1	alsin isoform X1
XM_009302176.3	XP_009300451.1	alsin isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa11483	XM_005165740.4 Latest version!	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa11484	XM_002662296.6 Latest version!	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa49117	XM_009302175.3 Latest version!	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa49118	XM_009302176.3 Latest version!	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa49119	XM_009302177.3 Latest version!	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa49119	XM_009302177.2	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa11483	XM_005165740.3	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa11484	XM_002662296.5	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa49117	XM_009302175.2	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa49118	XM_009302176.2	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

als2b ( XM_005165740.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165797.1 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa11483
Clone ID Related Accession (Same CDS sequence)	XM_005165740.3 , XM_005165740.4
Accession Version	XM_005165740.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	alsin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165740.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG 
AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT 
CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC 
CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA 
CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG 
GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT 
TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC 
CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG 
CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG 
CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA 
CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT 
GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT 
GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG 
CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG 
CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG 
GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA 
GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA 
GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA 
GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG 
GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA 
CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA 
AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC 
CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG 
CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC 
AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGTTTTCCAC CCATCACATT 
TTTCCATTGG CTACGCTGTG GGTGGAGCCA ATTTCTGAGG AGAGCACAGG CGTATATGGC 
TTAAAAGTGA CTTCGCCTGA GGAAAGCTTC GCTCTGCTGG CTTCATCACC AGCTGAAAAA 
GCAAAGTGGC TTCGTTCTAT AAACCAAGCG GTAGAGCAGG CGCTCAGTGG ATTGGGGCAT 
GATGGGATAC CTCCTTCTAC AGGGATGACG CAGAGAGCAG ATCCACCCAT CTCCCGGACA 
GCATCCTACA CCTTCTACAA AGACAGTCGG CTAAAGGATG CCAAATATGA TGGCCGATGG 
GTCTCAGGGA AACCCCATGG GCGGGGCGTG GTCAAATGGC CAGATGGACG AATGTACACG 
GGGACATTCA AGAACGGTTT TGAAGATGGT TTTGGAGATT ACGTTGTGTC CAATAAAACC 
CTGAACAGCT GTGATCATTA TCAAGGCCAG TGGAAAGATG GCAAGATGCA CGGTTTTGGA 
ACATTCAGAT ATGCCTCTGG TGAAGTGTAC GAAGGCTCAT TTCAAGACAA CATGCGTCAT 
GGTCATGGGA TGCTGCGTAG TGGGAAGCTG AATTCCACCT CTCCCAGTGT CTTTATCGGC 
CAGTGGCAGT ACGACAAAAA GTCTGGCTAT GGAGTTTTTG ATGACATCAC ACGAGGAGAG 
AAGTATATGG GCATGTGGCT GGATGATCAA CGGCAGGGGA ACGGAGTTGT TGTTACACAA 
TTTGGCCTAT ACTATGAAGG TGCATTCAGC AACAACAAAA TGATGGGTAC TGGGGTGTTA 
CTCTCTGAAG ATGACACTAC ATTTGAAGGA GAGTTTTTAG AAGACTGGAC TCTTAATGGA 
AAGGGTGTGT TGACCATGCC GAATGGAGAC TATATTGAAG GCTCTTTTTG TGGAGTTTGG 
GGAACCGGGC TAAAGATGTC AGGTTCCTAC TACAAACCCA GCCTGTACGA CTCAGACAAG 
GAGAAGGGTC ATGCACTTAA ACTGGGCCGG TTAGCAGTTC ACTCTGAAGA GAAGTGGAAG 
GCTGTGTTTT TGGAGTGCTG GAACAGGCTC GGTTGTGATT CCCCTGTACA GGGGCAAACC 
ACGACAGCGT GGGACAACAT TGCCATTGCA CTCACTGCTA ACCTGAGACA GCAGAGAGAC 
AGTCCAGAGT TGCTGAGCCG CTCACATCAC AAAACACTAG AGAGTCTTGA GTGCATCCCT 
CAACATGTGG GTCCTGTAAC ATTAGAGGTG TACCACACGA TCCGTCGGTA CCTGGTGAAG 
GCATGTGATA CTCCTCTGCA TCCTCTGGGT CGGCTGGTGG AGACGCTGGT TTCGGTATAC 
CGGATGACGT ATGTTGGTGT TGGAGCAAAC CGCAGGTTAT TACCGCAGGC CGTCAATGAG 
ATCACGTCCT ATCTCAGCCG TATCTTCCAG CTCGTCAGAT TTCTCTTTCC CGAACTGCCA 
GAAGAAGGAG GTTCACTAGC CGACCCTCCA AAAGAGAAGG TCGGCACAGA TGCTGATGGA 
AAGCTGCTGG AGTCTCCTAA ACCTGGGTGT GTGGTCAGCA GCTCTGCTCT CCTCCTGCCG 
GTGCTGTTGC CACGTCTCTA TCCTCCTCTC TTCACGCTCT ACGCTCTAGA GAAAGAGAAA 
GAGGACGATG TGTACTGGGA GTGTGTGCTG CGACTCAACA AACAGCCAGA TCTGGCACTG 
CTGGCTTTCC TCGGGGTCCA ACAGAAGTTC TGGCCTGTAA CTGTTACAAT ACATGGAGAA 
AAACAACAGG TTCTCTCAAG CACTAAAGAC GCCTGTTTCG CCTCAGCAGT TGAGACTTTG 
CAGCAAATAA GCACAACGTT CACACCCTCA GACAAGCTTC AGGTGATCCA GCTGACCTTC 
GAGGAAATAA CCCAGGAAGT GCTCGCACTC CTGAAACAGG ATTTCTTGTG GTCTATGGAT 
GACCTGTTTC CTGTCTTCCT CTTTGTTGTT CTGCGTGCAC GGATAAGGAA TCTGGGGTCA 
GAGGTTAGTC TCATTGAGGA CCTAATGGAT CCATGTGTAC AACATGGAGA GCATGGCATC 
ATGTTCACCA CCCTCAAGGC GTGCTACTAT CAAATCCAGC ATGAGAAGGT CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165797.1
CDS	250..5346
Translation	MEKLISSGEGDSSGERGLLHTWKGYSYSTAPERVLLSRPVLQAALGARHGVLLVEGGQVYSFGELPWKQNQTSSAEPILEAVLSEQRVVFVAAGSAHSGVVTEDGGVHMWGENVHGQCGLLGLSVIPNPTPVGVLDSEATPPQTVKILEVACGDQHTLALSAKHEVWAWGSGCQLGLNTSTFPVWKPQKVDHLSGRHVLQVACGSAHSVALVRCLPPPQEPRKPPQDKCGQCHQLLYTMTDKEDHVIISDGHHCPLGVEMDSTEIKSAVEGSPDPGKPGKSSPTEPVPCMSTQISKSPTSSNISESTRPPESTLPFELQNQVPAYCQESIEAPVEDSAAHEAPAAAGEVCVEASENSIPEPDSCRSSGKSPYPDEQAVKAYLKRLSDHALAEHTIKTSSTIHSAQLSGDPANLFASEPLIPVAQSVTPMGSALNNLVVSCASAVGERVVSTYEALSLRKVIGYWVPGEGRERVEERLRLEESMQGKKSSSLGDIREEEAELSRRLSLPGLLSQDNAESEVKASSSNTTTEVSPRLLRRTSRPRMRAVPLASGGIPETDAHLPSLQTEVWSWGQGQDGQLGHGDLLPRLQPDCVKSLNGKEVLKVAAGAHHSLALTAQSQVFSWGCNTSGQLGHMESPTTFPQLTKLSDGIRVWDIGAGLQHTLLLADGDCIQPILYYSGQQVKEVPEPTEEEEQTAEYTQQPVLLPFCMNLGYVSSVYAGGRTCAALTDCNVMGFIASLHELAAAERKYYCKLSNIKSLLLQPLLKLDSLSSALGQSSAGLLQNLIGRMSRLSQLTGQNSASLTSFLQRSRDIRGLVILEDAHLFLDTYSEYSSAVGDLLVMGGFQALVKPCHDVFGKGAEVVQRLSECSEEGLTLADVLAHLFYLPIKHLHEYGRLLLKLATCYEVSSVDYQKLQDACSKFESMALHLKRRRKEAEYTLHFWKSFPGKMTDSLRKPSRRLICESSNKALTLQNAGRFSVNWFILFNDALVHAQFSTHHIFPLATLWVEPISEESTGVYGLKVTSPEESFALLASSPAEKAKWLRSINQAVEQALSGLGHDGIPPSTGMTQRADPPISRTASYTFYKDSRLKDAKYDGRWVSGKPHGRGVVKWPDGRMYTGTFKNGFEDGFGDYVVSNKTLNSCDHYQGQWKDGKMHGFGTFRYASGEVYEGSFQDNMRHGHGMLRSGKLNSTSPSVFIGQWQYDKKSGYGVFDDITRGEKYMGMWLDDQRQGNGVVVTQFGLYYEGAFSNNKMMGTGVLLSEDDTTFEGEFLEDWTLNGKGVLTMPNGDYIEGSFCGVWGTGLKMSGSYYKPSLYDSDKEKGHALKLGRLAVHSEEKWKAVFLECWNRLGCDSPVQGQTTTAWDNIAIALTANLRQQRDSPELLSRSHHKTLESLECIPQHVGPVTLEVYHTIRRYLVKACDTPLHPLGRLVETLVSVYRMTYVGVGANRRLLPQAVNEITSYLSRIFQLVRFLFPELPEEGGSLADPPKEKVGTDADGKLLESPKPGCVVSSSALLLPVLLPRLYPPLFTLYALEKEKEDDVYWECVLRLNKQPDLALLAFLGVQQKFWPVTVTIHGEKQQVLSSTKDACFASAVETLQQISTTFTPSDKLQVIQLTFEEITQEVLALLKQDFLWSMDDLFPVFLFVVLRARIRNLGSEVSLIEDLMDPCVQHGEHGIMFTTLKACYYQIQHEKVT

Target ORF information:

RefSeq Version	XM_005165740.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165740.3

ORF Insert Sequence:

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG 
AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT 
CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC 
CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA 
CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG 
GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT 
TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC 
CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG 
CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG 
CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA 
CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT 
GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT 
GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG 
CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG 
CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG 
GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA 
GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA 
GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA 
GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG 
GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA 
CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA 
AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC 
CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG 
CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC 
AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGTTTTCCAC CCATCACATT 
TTTCCATTGG CTACGCTGTG GGTGGAGCCA ATTTCTGAGG AGAGCACAGG CGTATATGGC 
TTAAAAGTGA CTTCGCCTGA GGAAAGCTTC GCTCTGCTGG CTTCATCACC AGCTGAAAAA 
GCAAAGTGGC TTCGTTCTAT AAACCAAGCG GTAGAGCAGG CGCTCAGTGG ATTGGGGCAT 
GATGGGATAC CTCCTTCTAC AGGGATGACG CAGAGAGCAG ATCCACCCAT CTCCCGGACA 
GCATCCTACA CCTTCTACAA AGACAGTCGG CTAAAGGATG CCAAATATGA TGGCCGATGG 
GTCTCAGGGA AACCCCATGG GCGGGGCGTG GTCAAATGGC CAGATGGACG AATGTACACG 
GGGACATTCA AGAACGGTTT TGAAGATGGT TTTGGAGATT ACGTTGTGTC CAATAAAACC 
CTGAACAGCT GTGATCATTA TCAAGGCCAG TGGAAAGATG GCAAGATGCA CGGTTTTGGA 
ACATTCAGAT ATGCCTCTGG TGAAGTGTAC GAAGGCTCAT TTCAAGACAA CATGCGTCAT 
GGTCATGGGA TGCTGCGTAG TGGGAAGCTG AATTCCACCT CTCCCAGTGT CTTTATCGGC 
CAGTGGCAGT ACGACAAAAA GTCTGGCTAT GGAGTTTTTG ATGACATCAC ACGAGGAGAG 
AAGTATATGG GCATGTGGCT GGATGATCAA CGGCAGGGGA ACGGAGTTGT TGTTACACAA 
TTTGGCCTAT ACTATGAAGG TGCATTCAGC AACAACAAAA TGATGGGTAC TGGGGTGTTA 
CTCTCTGAAG ATGACACTAC ATTTGAAGGA GAGTTTTTAG AAGACTGGAC TCTTAATGGA 
AAGGGTGTGT TGACCATGCC GAATGGAGAC TATATTGAAG GCTCTTTTTG TGGAGTTTGG 
GGAACCGGGC TAAAGATGTC AGGTTCCTAC TACAAACCCA GCCTGTACGA CTCAGACAAG 
GAGAAGGGTC ATGCACTTAA ACTGGGCCGG TTAGCAGTTC ACTCTGAAGA GAAGTGGAAG 
GCTGTGTTTT TGGAGTGCTG GAACAGGCTC GGTTGTGATT CCCCTGTACA GGGGCAAACC 
ACGACAGCGT GGGACAACAT TGCCATTGCA CTCACTGCTA ACCTGAGACA GCAGAGAGAC 
AGTCCAGAGT TGCTGAGCCG CTCACATCAC AAAACACTAG AGAGTCTTGA GTGCATCCCT 
CAACATGTGG GTCCTGTAAC ATTAGAGGTG TACCACACGA TCCGTCGGTA CCTGGTGAAG 
GCATGTGATA CTCCTCTGCA TCCTCTGGGT CGGCTGGTGG AGACGCTGGT TTCGGTATAC 
CGGATGACGT ATGTTGGTGT TGGAGCAAAC CGCAGGTTAT TACCGCAGGC CGTCAATGAG 
ATCACGTCCT ATCTCAGCCG TATCTTCCAG CTCGTCAGAT TTCTCTTTCC CGAACTGCCA 
GAAGAAGGAG GTTCACTAGC CGACCCTCCA AAAGAGAAGG TCGGCACAGA TGCTGATGGA 
AAGCTGCTGG AGTCTCCTAA ACCTGGGTGT GTGGTCAGCA GCTCTGCTCT CCTCCTGCCG 
GTGCTGTTGC CACGTCTCTA TCCTCCTCTC TTCACGCTCT ACGCTCTAGA GAAAGAGAAA 
GAGGACGATG TGTACTGGGA GTGTGTGCTG CGACTCAACA AACAGCCAGA TCTGGCACTG 
CTGGCTTTCC TCGGGGTCCA ACAGAAGTTC TGGCCTGTAA CTGTTACAAT ACATGGAGAA 
AAACAACAGG TTCTCTCAAG CACTAAAGAC GCCTGTTTCG CCTCAGCAGT TGAGACTTTG 
CAGCAAATAA GCACAACGTT CACACCCTCA GACAAGCTTC AGGTGATCCA GCTGACCTTC 
GAGGAAATAA CCCAGGAAGT GCTCGCACTC CTGAAACAGG ATTTCTTGTG GTCTATGGAT 
GACCTGTTTC CTGTCTTCCT CTTTGTTGTT CTGCGTGCAC GGATAAGGAA TCTGGGGTCA 
GAGGTTAGTC TCATTGAGGA CCTAATGGAT CCATGTGTAC AACATGGAGA GCATGGCATC 
ATGTTCACCA CCCTCAAGGC GTGCTACTAT CAAATCCAGC ATGAGAAGGT CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

als2b ( XM_005165740.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165797.1 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa11483
Clone ID Related Accession (Same CDS sequence)	XM_005165740.3 , XM_005165740.4
Accession Version	XM_005165740.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	alsin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165740.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG 
AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT 
CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC 
CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA 
CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG 
GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT 
TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC 
CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG 
CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG 
CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA 
CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT 
GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT 
GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG 
CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG 
CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG 
GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA 
GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA 
GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA 
GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG 
GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA 
CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA 
AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC 
CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG 
CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC 
AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGTTTTCCAC CCATCACATT 
TTTCCATTGG CTACGCTGTG GGTGGAGCCA ATTTCTGAGG AGAGCACAGG CGTATATGGC 
TTAAAAGTGA CTTCGCCTGA GGAAAGCTTC GCTCTGCTGG CTTCATCACC AGCTGAAAAA 
GCAAAGTGGC TTCGTTCTAT AAACCAAGCG GTAGAGCAGG CGCTCAGTGG ATTGGGGCAT 
GATGGGATAC CTCCTTCTAC AGGGATGACG CAGAGAGCAG ATCCACCCAT CTCCCGGACA 
GCATCCTACA CCTTCTACAA AGACAGTCGG CTAAAGGATG CCAAATATGA TGGCCGATGG 
GTCTCAGGGA AACCCCATGG GCGGGGCGTG GTCAAATGGC CAGATGGACG AATGTACACG 
GGGACATTCA AGAACGGTTT TGAAGATGGT TTTGGAGATT ACGTTGTGTC CAATAAAACC 
CTGAACAGCT GTGATCATTA TCAAGGCCAG TGGAAAGATG GCAAGATGCA CGGTTTTGGA 
ACATTCAGAT ATGCCTCTGG TGAAGTGTAC GAAGGCTCAT TTCAAGACAA CATGCGTCAT 
GGTCATGGGA TGCTGCGTAG TGGGAAGCTG AATTCCACCT CTCCCAGTGT CTTTATCGGC 
CAGTGGCAGT ACGACAAAAA GTCTGGCTAT GGAGTTTTTG ATGACATCAC ACGAGGAGAG 
AAGTATATGG GCATGTGGCT GGATGATCAA CGGCAGGGGA ACGGAGTTGT TGTTACACAA 
TTTGGCCTAT ACTATGAAGG TGCATTCAGC AACAACAAAA TGATGGGTAC TGGGGTGTTA 
CTCTCTGAAG ATGACACTAC ATTTGAAGGA GAGTTTTTAG AAGACTGGAC TCTTAATGGA 
AAGGGTGTGT TGACCATGCC GAATGGAGAC TATATTGAAG GCTCTTTTTG TGGAGTTTGG 
GGAACCGGGC TAAAGATGTC AGGTTCCTAC TACAAACCCA GCCTGTACGA CTCAGACAAG 
GAGAAGGGTC ATGCACTTAA ACTGGGCCGG TTAGCAGTTC ACTCTGAAGA GAAGTGGAAG 
GCTGTGTTTT TGGAGTGCTG GAACAGGCTC GGTTGTGATT CCCCTGTACA GGGGCAAACC 
ACGACAGCGT GGGACAACAT TGCCATTGCA CTCACTGCTA ACCTGAGACA GCAGAGAGAC 
AGTCCAGAGT TGCTGAGCCG CTCACATCAC AAAACACTAG AGAGTCTTGA GTGCATCCCT 
CAACATGTGG GTCCTGTAAC ATTAGAGGTG TACCACACGA TCCGTCGGTA CCTGGTGAAG 
GCATGTGATA CTCCTCTGCA TCCTCTGGGT CGGCTGGTGG AGACGCTGGT TTCGGTATAC 
CGGATGACGT ATGTTGGTGT TGGAGCAAAC CGCAGGTTAT TACCGCAGGC CGTCAATGAG 
ATCACGTCCT ATCTCAGCCG TATCTTCCAG CTCGTCAGAT TTCTCTTTCC CGAACTGCCA 
GAAGAAGGAG GTTCACTAGC CGACCCTCCA AAAGAGAAGG TCGGCACAGA TGCTGATGGA 
AAGCTGCTGG AGTCTCCTAA ACCTGGGTGT GTGGTCAGCA GCTCTGCTCT CCTCCTGCCG 
GTGCTGTTGC CACGTCTCTA TCCTCCTCTC TTCACGCTCT ACGCTCTAGA GAAAGAGAAA 
GAGGACGATG TGTACTGGGA GTGTGTGCTG CGACTCAACA AACAGCCAGA TCTGGCACTG 
CTGGCTTTCC TCGGGGTCCA ACAGAAGTTC TGGCCTGTAA CTGTTACAAT ACATGGAGAA 
AAACAACAGG TTCTCTCAAG CACTAAAGAC GCCTGTTTCG CCTCAGCAGT TGAGACTTTG 
CAGCAAATAA GCACAACGTT CACACCCTCA GACAAGCTTC AGGTGATCCA GCTGACCTTC 
GAGGAAATAA CCCAGGAAGT GCTCGCACTC CTGAAACAGG ATTTCTTGTG GTCTATGGAT 
GACCTGTTTC CTGTCTTCCT CTTTGTTGTT CTGCGTGCAC GGATAAGGAA TCTGGGGTCA 
GAGGTTAGTC TCATTGAGGA CCTAATGGAT CCATGTGTAC AACATGGAGA GCATGGCATC 
ATGTTCACCA CCCTCAAGGC GTGCTACTAT CAAATCCAGC ATGAGAAGGT CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165797.1
CDS	270..5366
Translation	MEKLISSGEGDSSGERGLLHTWKGYSYSTAPERVLLSRPVLQAALGARHGVLLVEGGQVYSFGELPWKQNQTSSAEPILEAVLSEQRVVFVAAGSAHSGVVTEDGGVHMWGENVHGQCGLLGLSVIPNPTPVGVLDSEATPPQTVKILEVACGDQHTLALSAKHEVWAWGSGCQLGLNTSTFPVWKPQKVDHLSGRHVLQVACGSAHSVALVRCLPPPQEPRKPPQDKCGQCHQLLYTMTDKEDHVIISDGHHCPLGVEMDSTEIKSAVEGSPDPGKPGKSSPTEPVPCMSTQISKSPTSSNISESTRPPESTLPFELQNQVPAYCQESIEAPVEDSAAHEAPAAAGEVCVEASENSIPEPDSCRSSGKSPYPDEQAVKAYLKRLSDHALAEHTIKTSSTIHSAQLSGDPANLFASEPLIPVAQSVTPMGSALNNLVVSCASAVGERVVSTYEALSLRKVIGYWVPGEGRERVEERLRLEESMQGKKSSSLGDIREEEAELSRRLSLPGLLSQDNAESEVKASSSNTTTEVSPRLLRRTSRPRMRAVPLASGGIPETDAHLPSLQTEVWSWGQGQDGQLGHGDLLPRLQPDCVKSLNGKEVLKVAAGAHHSLALTAQSQVFSWGCNTSGQLGHMESPTTFPQLTKLSDGIRVWDIGAGLQHTLLLADGDCIQPILYYSGQQVKEVPEPTEEEEQTAEYTQQPVLLPFCMNLGYVSSVYAGGRTCAALTDCNVMGFIASLHELAAAERKYYCKLSNIKSLLLQPLLKLDSLSSALGQSSAGLLQNLIGRMSRLSQLTGQNSASLTSFLQRSRDIRGLVILEDAHLFLDTYSEYSSAVGDLLVMGGFQALVKPCHDVFGKGAEVVQRLSECSEEGLTLADVLAHLFYLPIKHLHEYGRLLLKLATCYEVSSVDYQKLQDACSKFESMALHLKRRRKEAEYTLHFWKSFPGKMTDSLRKPSRRLICESSNKALTLQNAGRFSVNWFILFNDALVHAQFSTHHIFPLATLWVEPISEESTGVYGLKVTSPEESFALLASSPAEKAKWLRSINQAVEQALSGLGHDGIPPSTGMTQRADPPISRTASYTFYKDSRLKDAKYDGRWVSGKPHGRGVVKWPDGRMYTGTFKNGFEDGFGDYVVSNKTLNSCDHYQGQWKDGKMHGFGTFRYASGEVYEGSFQDNMRHGHGMLRSGKLNSTSPSVFIGQWQYDKKSGYGVFDDITRGEKYMGMWLDDQRQGNGVVVTQFGLYYEGAFSNNKMMGTGVLLSEDDTTFEGEFLEDWTLNGKGVLTMPNGDYIEGSFCGVWGTGLKMSGSYYKPSLYDSDKEKGHALKLGRLAVHSEEKWKAVFLECWNRLGCDSPVQGQTTTAWDNIAIALTANLRQQRDSPELLSRSHHKTLESLECIPQHVGPVTLEVYHTIRRYLVKACDTPLHPLGRLVETLVSVYRMTYVGVGANRRLLPQAVNEITSYLSRIFQLVRFLFPELPEEGGSLADPPKEKVGTDADGKLLESPKPGCVVSSSALLLPVLLPRLYPPLFTLYALEKEKEDDVYWECVLRLNKQPDLALLAFLGVQQKFWPVTVTIHGEKQQVLSSTKDACFASAVETLQQISTTFTPSDKLQVIQLTFEEITQEVLALLKQDFLWSMDDLFPVFLFVVLRARIRNLGSEVSLIEDLMDPCVQHGEHGIMFTTLKACYYQIQHEKVT

Target ORF information:

RefSeq Version	XM_005165740.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165740.4

ORF Insert Sequence:

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG 
AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT 
CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC 
CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA 
CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG 
GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT 
TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC 
CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG 
CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG 
CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA 
CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT 
GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT 
GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG 
CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG 
CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG 
GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA 
GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA 
GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA 
GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG 
GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA 
CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA 
AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC 
CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG 
CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC 
AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGTTTTCCAC CCATCACATT 
TTTCCATTGG CTACGCTGTG GGTGGAGCCA ATTTCTGAGG AGAGCACAGG CGTATATGGC 
TTAAAAGTGA CTTCGCCTGA GGAAAGCTTC GCTCTGCTGG CTTCATCACC AGCTGAAAAA 
GCAAAGTGGC TTCGTTCTAT AAACCAAGCG GTAGAGCAGG CGCTCAGTGG ATTGGGGCAT 
GATGGGATAC CTCCTTCTAC AGGGATGACG CAGAGAGCAG ATCCACCCAT CTCCCGGACA 
GCATCCTACA CCTTCTACAA AGACAGTCGG CTAAAGGATG CCAAATATGA TGGCCGATGG 
GTCTCAGGGA AACCCCATGG GCGGGGCGTG GTCAAATGGC CAGATGGACG AATGTACACG 
GGGACATTCA AGAACGGTTT TGAAGATGGT TTTGGAGATT ACGTTGTGTC CAATAAAACC 
CTGAACAGCT GTGATCATTA TCAAGGCCAG TGGAAAGATG GCAAGATGCA CGGTTTTGGA 
ACATTCAGAT ATGCCTCTGG TGAAGTGTAC GAAGGCTCAT TTCAAGACAA CATGCGTCAT 
GGTCATGGGA TGCTGCGTAG TGGGAAGCTG AATTCCACCT CTCCCAGTGT CTTTATCGGC 
CAGTGGCAGT ACGACAAAAA GTCTGGCTAT GGAGTTTTTG ATGACATCAC ACGAGGAGAG 
AAGTATATGG GCATGTGGCT GGATGATCAA CGGCAGGGGA ACGGAGTTGT TGTTACACAA 
TTTGGCCTAT ACTATGAAGG TGCATTCAGC AACAACAAAA TGATGGGTAC TGGGGTGTTA 
CTCTCTGAAG ATGACACTAC ATTTGAAGGA GAGTTTTTAG AAGACTGGAC TCTTAATGGA 
AAGGGTGTGT TGACCATGCC GAATGGAGAC TATATTGAAG GCTCTTTTTG TGGAGTTTGG 
GGAACCGGGC TAAAGATGTC AGGTTCCTAC TACAAACCCA GCCTGTACGA CTCAGACAAG 
GAGAAGGGTC ATGCACTTAA ACTGGGCCGG TTAGCAGTTC ACTCTGAAGA GAAGTGGAAG 
GCTGTGTTTT TGGAGTGCTG GAACAGGCTC GGTTGTGATT CCCCTGTACA GGGGCAAACC 
ACGACAGCGT GGGACAACAT TGCCATTGCA CTCACTGCTA ACCTGAGACA GCAGAGAGAC 
AGTCCAGAGT TGCTGAGCCG CTCACATCAC AAAACACTAG AGAGTCTTGA GTGCATCCCT 
CAACATGTGG GTCCTGTAAC ATTAGAGGTG TACCACACGA TCCGTCGGTA CCTGGTGAAG 
GCATGTGATA CTCCTCTGCA TCCTCTGGGT CGGCTGGTGG AGACGCTGGT TTCGGTATAC 
CGGATGACGT ATGTTGGTGT TGGAGCAAAC CGCAGGTTAT TACCGCAGGC CGTCAATGAG 
ATCACGTCCT ATCTCAGCCG TATCTTCCAG CTCGTCAGAT TTCTCTTTCC CGAACTGCCA 
GAAGAAGGAG GTTCACTAGC CGACCCTCCA AAAGAGAAGG TCGGCACAGA TGCTGATGGA 
AAGCTGCTGG AGTCTCCTAA ACCTGGGTGT GTGGTCAGCA GCTCTGCTCT CCTCCTGCCG 
GTGCTGTTGC CACGTCTCTA TCCTCCTCTC TTCACGCTCT ACGCTCTAGA GAAAGAGAAA 
GAGGACGATG TGTACTGGGA GTGTGTGCTG CGACTCAACA AACAGCCAGA TCTGGCACTG 
CTGGCTTTCC TCGGGGTCCA ACAGAAGTTC TGGCCTGTAA CTGTTACAAT ACATGGAGAA 
AAACAACAGG TTCTCTCAAG CACTAAAGAC GCCTGTTTCG CCTCAGCAGT TGAGACTTTG 
CAGCAAATAA GCACAACGTT CACACCCTCA GACAAGCTTC AGGTGATCCA GCTGACCTTC 
GAGGAAATAA CCCAGGAAGT GCTCGCACTC CTGAAACAGG ATTTCTTGTG GTCTATGGAT 
GACCTGTTTC CTGTCTTCCT CTTTGTTGTT CTGCGTGCAC GGATAAGGAA TCTGGGGTCA 
GAGGTTAGTC TCATTGAGGA CCTAATGGAT CCATGTGTAC AACATGGAGA GCATGGCATC 
ATGTTCACCA CCCTCAAGGC GTGCTACTAT CAAATCCAGC ATGAGAAGGT CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

als2b ( XM_002662296.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002662342.2 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X5, mRNA.

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CloneID	ODa11484
Clone ID Related Accession (Same CDS sequence)	XM_002662296.5 , XM_002662296.6
Accession Version	XM_002662296.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	alsin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002662296.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG 
AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT 
GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA 
CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT 
GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG 
TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC 
ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA 
GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC 
AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA 
TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG 
TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT 
AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG 
AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA 
TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT 
CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT 
ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT 
GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG 
AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA 
GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG 
CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT 
GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA 
TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG 
TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT 
TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GTTTTCCACC 
CATCACATTT TTCCATTGGC TACGCTGTGG GTGGAGCCAA TTTCTGAGGA GAGCACAGGC 
GTATATGGCT TAAAAGTGAC TTCGCCTGAG GAAAGCTTCG CTCTGCTGGC TTCATCACCA 
GCTGAAAAAG CAAAGTGGCT TCGTTCTATA AACCAAGCGG TAGAGCAGGC GCTCAGTGGA 
TTGGGGCATG ATGGGATACC TCCTTCTACA GGGATGACGC AGAGAGCAGA TCCACCCATC 
TCCCGGACAG CATCCTACAC CTTCTACAAA GACAGTCGGC TAAAGGATGC CAAATATGAT 
GGCCGATGGG TCTCAGGGAA ACCCCATGGG CGGGGCGTGG TCAAATGGCC AGATGGACGA 
ATGTACACGG GGACATTCAA GAACGGTTTT GAAGATGGTT TTGGAGATTA CGTTGTGTCC 
AATAAAACCC TGAACAGCTG TGATCATTAT CAAGGCCAGT GGAAAGATGG CAAGATGCAC 
GGTTTTGGAA CATTCAGATA TGCCTCTGGT GAAGTGTACG AAGGCTCATT TCAAGACAAC 
ATGCGTCATG GTCATGGGAT GCTGCGTAGT GGGAAGCTGA ATTCCACCTC TCCCAGTGTC 
TTTATCGGCC AGTGGCAGTA CGACAAAAAG TCTGGCTATG GAGTTTTTGA TGACATCACA 
CGAGGAGAGA AGTATATGGG CATGTGGCTG GATGATCAAC GGCAGGGGAA CGGAGTTGTT 
GTTACACAAT TTGGCCTATA CTATGAAGGT GCATTCAGCA ACAACAAAAT GATGGGTACT 
GGGGTGTTAC TCTCTGAAGA TGACACTACA TTTGAAGGAG AGTTTTTAGA AGACTGGACT 
CTTAATGGAA AGGGTGTGTT GACCATGCCG AATGGAGACT ATATTGAAGG CTCTTTTTGT 
GGAGTTTGGG GAACCGGGCT AAAGATGTCA GGTTCCTACT ACAAACCCAG CCTGTACGAC 
TCAGACAAGG AGAAGGGTCA TGCACTTAAA CTGGGCCGGT TAGCAGTTCA CTCTGAAGAG 
AAGTGGAAGG CTGTGTTTTT GGAGTGCTGG AACAGGCTCG GTTGTGATTC CCCTGTACAG 
GGGCAAACCA CGACAGCGTG GGACAACATT GCCATTGCAC TCACTGCTAA CCTGAGACAG 
CAGAGAGACA GTCCAGAGTT GCTGAGCCGC TCACATCACA AAACACTAGA GAGTCTTGAG 
TGCATCCCTC AACATGTGGG TCCTGTAACA TTAGAGGTGT ACCACACGAT CCGTCGGTAC 
CTGGTGAAGG CATGTGATAC TCCTCTGCAT CCTCTGGGTC GGCTGGTGGA GACGCTGGTT 
TCGGTATACC GGATGACGTA TGTTGGTGTT GGAGCAAACC GCAGGTTATT ACCGCAGGCC 
GTCAATGAGA TCACGTCCTA TCTCAGCCGT ATCTTCCAGC TCGTCAGATT TCTCTTTCCC 
GAACTGCCAG AAGAAGGAGG TTCACTAGCC GACCCTCCAA AAGAGAAGGT CGGCACAGAT 
GCTGATGGAA AGCTGCTGGA GTCTCCTAAA CCTGGGTGTG TGGTCAGCAG CTCTGCTCTC 
CTCCTGCCGG TGCTGTTGCC ACGTCTCTAT CCTCCTCTCT TCACGCTCTA CGCTCTAGAG 
AAAGAGAAAG AGGACGATGT GTACTGGGAG TGTGTGCTGC GACTCAACAA ACAGCCAGAT 
CTGGCACTGC TGGCTTTCCT CGGGGTCCAA CAGAAGTTCT GGCCTGTAAC TGTTACAATA 
CATGGAGAAA AACAACAGGT TCTCTCAAGC ACTAAAGACG CCTGTTTCGC CTCAGCAGTT 
GAGACTTTGC AGCAAATAAG CACAACGTTC ACACCCTCAG ACAAGCTTCA GGTGATCCAG 
CTGACCTTCG AGGAAATAAC CCAGGAAGTG CTCGCACTCC TGAAACAGGA TTTCTTGTGG 
TCTATGGATG ACCTGTTTCC TGTCTTCCTC TTTGTTGTTC TGCGTGCACG GATAAGGAAT 
CTGGGGTCAG AGGTTAGTCT CATTGAGGAC CTAATGGATC CATGTGTACA ACATGGAGAG 
CATGGCATCA TGTTCACCAC CCTCAAGGCG TGCTACTATC AAATCCAGCA TGAGAAGGTC 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002662342.2
CDS	250..5295
Translation	MEKLISSGEGDSSGERGLLHTWKGYSYSTAPERVLLSRPVLQAALGARHGVLLVEGGQVYSFGELPWKQNQTSSAEPILEAVLSEQRVVFVAAGSAHSGVVTEDGGVHMWGENVHGQCGLLGLSVIPNPTPVGVLDSEATPPQTVKILEVACGDQHTLALSAKHEVWAWGSGCQLGLNTSTFPVWKPQKVDHLSGRHVLQVACGSAHSVALVRCLPPPQEPRKPPQDKCGQCHQLLYTMTDKEDHVIISDGHHCPLGVEMDSTEIKSAVEGSPDPGKPGKSSPTEPVPCMSTQISKSPTSSNISESTRPPESTLPFELQNQVPAYCQESIEAPVEDSAAHEAPAAAGEVCVEASENSIPEPDSCRSSGKSPYPDEQAVKAYLKRLSDHALAEHTIKTSSTIHSAQLSGDPANLFASEPLIPVAQSVTPMGSALNNLVVSCASAVGERVVSTYEALSLRKVIGYWVPGEGRERVEERLRLEESMQGKKSSSLGDIREEEAELSRRLSLPGLLSQVSPRLLRRTSRPRMRAVPLASGGIPETDAHLPSLQTEVWSWGQGQDGQLGHGDLLPRLQPDCVKSLNGKEVLKVAAGAHHSLALTAQSQVFSWGCNTSGQLGHMESPTTFPQLTKLSDGIRVWDIGAGLQHTLLLADGDCIQPILYYSGQQVKEVPEPTEEEEQTAEYTQQPVLLPFCMNLGYVSSVYAGGRTCAALTDCNVMGFIASLHELAAAERKYYCKLSNIKSLLLQPLLKLDSLSSALGQSSAGLLQNLIGRMSRLSQLTGQNSASLTSFLQRSRDIRGLVILEDAHLFLDTYSEYSSAVGDLLVMGGFQALVKPCHDVFGKGAEVVQRLSECSEEGLTLADVLAHLFYLPIKHLHEYGRLLLKLATCYEVSSVDYQKLQDACSKFESMALHLKRRRKEAEYTLHFWKSFPGKMTDSLRKPSRRLICESSNKALTLQNAGRFSVNWFILFNDALVHAQFSTHHIFPLATLWVEPISEESTGVYGLKVTSPEESFALLASSPAEKAKWLRSINQAVEQALSGLGHDGIPPSTGMTQRADPPISRTASYTFYKDSRLKDAKYDGRWVSGKPHGRGVVKWPDGRMYTGTFKNGFEDGFGDYVVSNKTLNSCDHYQGQWKDGKMHGFGTFRYASGEVYEGSFQDNMRHGHGMLRSGKLNSTSPSVFIGQWQYDKKSGYGVFDDITRGEKYMGMWLDDQRQGNGVVVTQFGLYYEGAFSNNKMMGTGVLLSEDDTTFEGEFLEDWTLNGKGVLTMPNGDYIEGSFCGVWGTGLKMSGSYYKPSLYDSDKEKGHALKLGRLAVHSEEKWKAVFLECWNRLGCDSPVQGQTTTAWDNIAIALTANLRQQRDSPELLSRSHHKTLESLECIPQHVGPVTLEVYHTIRRYLVKACDTPLHPLGRLVETLVSVYRMTYVGVGANRRLLPQAVNEITSYLSRIFQLVRFLFPELPEEGGSLADPPKEKVGTDADGKLLESPKPGCVVSSSALLLPVLLPRLYPPLFTLYALEKEKEDDVYWECVLRLNKQPDLALLAFLGVQQKFWPVTVTIHGEKQQVLSSTKDACFASAVETLQQISTTFTPSDKLQVIQLTFEEITQEVLALLKQDFLWSMDDLFPVFLFVVLRARIRNLGSEVSLIEDLMDPCVQHGEHGIMFTTLKACYYQIQHEKVT

Target ORF information:

RefSeq Version	XM_002662296.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002662296.5

ORF Insert Sequence:

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG 
AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT 
GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA 
CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT 
GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG 
TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC 
ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA 
GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC 
AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA 
TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG 
TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT 
AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG 
AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA 
TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT 
CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT 
ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT 
GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG 
AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA 
GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG 
CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT 
GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA 
TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG 
TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT 
TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GTTTTCCACC 
CATCACATTT TTCCATTGGC TACGCTGTGG GTGGAGCCAA TTTCTGAGGA GAGCACAGGC 
GTATATGGCT TAAAAGTGAC TTCGCCTGAG GAAAGCTTCG CTCTGCTGGC TTCATCACCA 
GCTGAAAAAG CAAAGTGGCT TCGTTCTATA AACCAAGCGG TAGAGCAGGC GCTCAGTGGA 
TTGGGGCATG ATGGGATACC TCCTTCTACA GGGATGACGC AGAGAGCAGA TCCACCCATC 
TCCCGGACAG CATCCTACAC CTTCTACAAA GACAGTCGGC TAAAGGATGC CAAATATGAT 
GGCCGATGGG TCTCAGGGAA ACCCCATGGG CGGGGCGTGG TCAAATGGCC AGATGGACGA 
ATGTACACGG GGACATTCAA GAACGGTTTT GAAGATGGTT TTGGAGATTA CGTTGTGTCC 
AATAAAACCC TGAACAGCTG TGATCATTAT CAAGGCCAGT GGAAAGATGG CAAGATGCAC 
GGTTTTGGAA CATTCAGATA TGCCTCTGGT GAAGTGTACG AAGGCTCATT TCAAGACAAC 
ATGCGTCATG GTCATGGGAT GCTGCGTAGT GGGAAGCTGA ATTCCACCTC TCCCAGTGTC 
TTTATCGGCC AGTGGCAGTA CGACAAAAAG TCTGGCTATG GAGTTTTTGA TGACATCACA 
CGAGGAGAGA AGTATATGGG CATGTGGCTG GATGATCAAC GGCAGGGGAA CGGAGTTGTT 
GTTACACAAT TTGGCCTATA CTATGAAGGT GCATTCAGCA ACAACAAAAT GATGGGTACT 
GGGGTGTTAC TCTCTGAAGA TGACACTACA TTTGAAGGAG AGTTTTTAGA AGACTGGACT 
CTTAATGGAA AGGGTGTGTT GACCATGCCG AATGGAGACT ATATTGAAGG CTCTTTTTGT 
GGAGTTTGGG GAACCGGGCT AAAGATGTCA GGTTCCTACT ACAAACCCAG CCTGTACGAC 
TCAGACAAGG AGAAGGGTCA TGCACTTAAA CTGGGCCGGT TAGCAGTTCA CTCTGAAGAG 
AAGTGGAAGG CTGTGTTTTT GGAGTGCTGG AACAGGCTCG GTTGTGATTC CCCTGTACAG 
GGGCAAACCA CGACAGCGTG GGACAACATT GCCATTGCAC TCACTGCTAA CCTGAGACAG 
CAGAGAGACA GTCCAGAGTT GCTGAGCCGC TCACATCACA AAACACTAGA GAGTCTTGAG 
TGCATCCCTC AACATGTGGG TCCTGTAACA TTAGAGGTGT ACCACACGAT CCGTCGGTAC 
CTGGTGAAGG CATGTGATAC TCCTCTGCAT CCTCTGGGTC GGCTGGTGGA GACGCTGGTT 
TCGGTATACC GGATGACGTA TGTTGGTGTT GGAGCAAACC GCAGGTTATT ACCGCAGGCC 
GTCAATGAGA TCACGTCCTA TCTCAGCCGT ATCTTCCAGC TCGTCAGATT TCTCTTTCCC 
GAACTGCCAG AAGAAGGAGG TTCACTAGCC GACCCTCCAA AAGAGAAGGT CGGCACAGAT 
GCTGATGGAA AGCTGCTGGA GTCTCCTAAA CCTGGGTGTG TGGTCAGCAG CTCTGCTCTC 
CTCCTGCCGG TGCTGTTGCC ACGTCTCTAT CCTCCTCTCT TCACGCTCTA CGCTCTAGAG 
AAAGAGAAAG AGGACGATGT GTACTGGGAG TGTGTGCTGC GACTCAACAA ACAGCCAGAT 
CTGGCACTGC TGGCTTTCCT CGGGGTCCAA CAGAAGTTCT GGCCTGTAAC TGTTACAATA 
CATGGAGAAA AACAACAGGT TCTCTCAAGC ACTAAAGACG CCTGTTTCGC CTCAGCAGTT 
GAGACTTTGC AGCAAATAAG CACAACGTTC ACACCCTCAG ACAAGCTTCA GGTGATCCAG 
CTGACCTTCG AGGAAATAAC CCAGGAAGTG CTCGCACTCC TGAAACAGGA TTTCTTGTGG 
TCTATGGATG ACCTGTTTCC TGTCTTCCTC TTTGTTGTTC TGCGTGCACG GATAAGGAAT 
CTGGGGTCAG AGGTTAGTCT CATTGAGGAC CTAATGGATC CATGTGTACA ACATGGAGAG 
CATGGCATCA TGTTCACCAC CCTCAAGGCG TGCTACTATC AAATCCAGCA TGAGAAGGTC 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

als2b ( XM_002662296.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002662342.2 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X5, mRNA.

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CloneID	ODa11484
Clone ID Related Accession (Same CDS sequence)	XM_002662296.5 , XM_002662296.6
Accession Version	XM_002662296.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	alsin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002662296.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG 
AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT 
GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA 
CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT 
GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG 
TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC 
ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA 
GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC 
AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA 
TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG 
TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT 
AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG 
AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA 
TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT 
CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT 
ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT 
GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG 
AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA 
GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG 
CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT 
GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA 
TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG 
TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT 
TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GTTTTCCACC 
CATCACATTT TTCCATTGGC TACGCTGTGG GTGGAGCCAA TTTCTGAGGA GAGCACAGGC 
GTATATGGCT TAAAAGTGAC TTCGCCTGAG GAAAGCTTCG CTCTGCTGGC TTCATCACCA 
GCTGAAAAAG CAAAGTGGCT TCGTTCTATA AACCAAGCGG TAGAGCAGGC GCTCAGTGGA 
TTGGGGCATG ATGGGATACC TCCTTCTACA GGGATGACGC AGAGAGCAGA TCCACCCATC 
TCCCGGACAG CATCCTACAC CTTCTACAAA GACAGTCGGC TAAAGGATGC CAAATATGAT 
GGCCGATGGG TCTCAGGGAA ACCCCATGGG CGGGGCGTGG TCAAATGGCC AGATGGACGA 
ATGTACACGG GGACATTCAA GAACGGTTTT GAAGATGGTT TTGGAGATTA CGTTGTGTCC 
AATAAAACCC TGAACAGCTG TGATCATTAT CAAGGCCAGT GGAAAGATGG CAAGATGCAC 
GGTTTTGGAA CATTCAGATA TGCCTCTGGT GAAGTGTACG AAGGCTCATT TCAAGACAAC 
ATGCGTCATG GTCATGGGAT GCTGCGTAGT GGGAAGCTGA ATTCCACCTC TCCCAGTGTC 
TTTATCGGCC AGTGGCAGTA CGACAAAAAG TCTGGCTATG GAGTTTTTGA TGACATCACA 
CGAGGAGAGA AGTATATGGG CATGTGGCTG GATGATCAAC GGCAGGGGAA CGGAGTTGTT 
GTTACACAAT TTGGCCTATA CTATGAAGGT GCATTCAGCA ACAACAAAAT GATGGGTACT 
GGGGTGTTAC TCTCTGAAGA TGACACTACA TTTGAAGGAG AGTTTTTAGA AGACTGGACT 
CTTAATGGAA AGGGTGTGTT GACCATGCCG AATGGAGACT ATATTGAAGG CTCTTTTTGT 
GGAGTTTGGG GAACCGGGCT AAAGATGTCA GGTTCCTACT ACAAACCCAG CCTGTACGAC 
TCAGACAAGG AGAAGGGTCA TGCACTTAAA CTGGGCCGGT TAGCAGTTCA CTCTGAAGAG 
AAGTGGAAGG CTGTGTTTTT GGAGTGCTGG AACAGGCTCG GTTGTGATTC CCCTGTACAG 
GGGCAAACCA CGACAGCGTG GGACAACATT GCCATTGCAC TCACTGCTAA CCTGAGACAG 
CAGAGAGACA GTCCAGAGTT GCTGAGCCGC TCACATCACA AAACACTAGA GAGTCTTGAG 
TGCATCCCTC AACATGTGGG TCCTGTAACA TTAGAGGTGT ACCACACGAT CCGTCGGTAC 
CTGGTGAAGG CATGTGATAC TCCTCTGCAT CCTCTGGGTC GGCTGGTGGA GACGCTGGTT 
TCGGTATACC GGATGACGTA TGTTGGTGTT GGAGCAAACC GCAGGTTATT ACCGCAGGCC 
GTCAATGAGA TCACGTCCTA TCTCAGCCGT ATCTTCCAGC TCGTCAGATT TCTCTTTCCC 
GAACTGCCAG AAGAAGGAGG TTCACTAGCC GACCCTCCAA AAGAGAAGGT CGGCACAGAT 
GCTGATGGAA AGCTGCTGGA GTCTCCTAAA CCTGGGTGTG TGGTCAGCAG CTCTGCTCTC 
CTCCTGCCGG TGCTGTTGCC ACGTCTCTAT CCTCCTCTCT TCACGCTCTA CGCTCTAGAG 
AAAGAGAAAG AGGACGATGT GTACTGGGAG TGTGTGCTGC GACTCAACAA ACAGCCAGAT 
CTGGCACTGC TGGCTTTCCT CGGGGTCCAA CAGAAGTTCT GGCCTGTAAC TGTTACAATA 
CATGGAGAAA AACAACAGGT TCTCTCAAGC ACTAAAGACG CCTGTTTCGC CTCAGCAGTT 
GAGACTTTGC AGCAAATAAG CACAACGTTC ACACCCTCAG ACAAGCTTCA GGTGATCCAG 
CTGACCTTCG AGGAAATAAC CCAGGAAGTG CTCGCACTCC TGAAACAGGA TTTCTTGTGG 
TCTATGGATG ACCTGTTTCC TGTCTTCCTC TTTGTTGTTC TGCGTGCACG GATAAGGAAT 
CTGGGGTCAG AGGTTAGTCT CATTGAGGAC CTAATGGATC CATGTGTACA ACATGGAGAG 
CATGGCATCA TGTTCACCAC CCTCAAGGCG TGCTACTATC AAATCCAGCA TGAGAAGGTC 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002662342.2
CDS	270..5315
Translation	MEKLISSGEGDSSGERGLLHTWKGYSYSTAPERVLLSRPVLQAALGARHGVLLVEGGQVYSFGELPWKQNQTSSAEPILEAVLSEQRVVFVAAGSAHSGVVTEDGGVHMWGENVHGQCGLLGLSVIPNPTPVGVLDSEATPPQTVKILEVACGDQHTLALSAKHEVWAWGSGCQLGLNTSTFPVWKPQKVDHLSGRHVLQVACGSAHSVALVRCLPPPQEPRKPPQDKCGQCHQLLYTMTDKEDHVIISDGHHCPLGVEMDSTEIKSAVEGSPDPGKPGKSSPTEPVPCMSTQISKSPTSSNISESTRPPESTLPFELQNQVPAYCQESIEAPVEDSAAHEAPAAAGEVCVEASENSIPEPDSCRSSGKSPYPDEQAVKAYLKRLSDHALAEHTIKTSSTIHSAQLSGDPANLFASEPLIPVAQSVTPMGSALNNLVVSCASAVGERVVSTYEALSLRKVIGYWVPGEGRERVEERLRLEESMQGKKSSSLGDIREEEAELSRRLSLPGLLSQVSPRLLRRTSRPRMRAVPLASGGIPETDAHLPSLQTEVWSWGQGQDGQLGHGDLLPRLQPDCVKSLNGKEVLKVAAGAHHSLALTAQSQVFSWGCNTSGQLGHMESPTTFPQLTKLSDGIRVWDIGAGLQHTLLLADGDCIQPILYYSGQQVKEVPEPTEEEEQTAEYTQQPVLLPFCMNLGYVSSVYAGGRTCAALTDCNVMGFIASLHELAAAERKYYCKLSNIKSLLLQPLLKLDSLSSALGQSSAGLLQNLIGRMSRLSQLTGQNSASLTSFLQRSRDIRGLVILEDAHLFLDTYSEYSSAVGDLLVMGGFQALVKPCHDVFGKGAEVVQRLSECSEEGLTLADVLAHLFYLPIKHLHEYGRLLLKLATCYEVSSVDYQKLQDACSKFESMALHLKRRRKEAEYTLHFWKSFPGKMTDSLRKPSRRLICESSNKALTLQNAGRFSVNWFILFNDALVHAQFSTHHIFPLATLWVEPISEESTGVYGLKVTSPEESFALLASSPAEKAKWLRSINQAVEQALSGLGHDGIPPSTGMTQRADPPISRTASYTFYKDSRLKDAKYDGRWVSGKPHGRGVVKWPDGRMYTGTFKNGFEDGFGDYVVSNKTLNSCDHYQGQWKDGKMHGFGTFRYASGEVYEGSFQDNMRHGHGMLRSGKLNSTSPSVFIGQWQYDKKSGYGVFDDITRGEKYMGMWLDDQRQGNGVVVTQFGLYYEGAFSNNKMMGTGVLLSEDDTTFEGEFLEDWTLNGKGVLTMPNGDYIEGSFCGVWGTGLKMSGSYYKPSLYDSDKEKGHALKLGRLAVHSEEKWKAVFLECWNRLGCDSPVQGQTTTAWDNIAIALTANLRQQRDSPELLSRSHHKTLESLECIPQHVGPVTLEVYHTIRRYLVKACDTPLHPLGRLVETLVSVYRMTYVGVGANRRLLPQAVNEITSYLSRIFQLVRFLFPELPEEGGSLADPPKEKVGTDADGKLLESPKPGCVVSSSALLLPVLLPRLYPPLFTLYALEKEKEDDVYWECVLRLNKQPDLALLAFLGVQQKFWPVTVTIHGEKQQVLSSTKDACFASAVETLQQISTTFTPSDKLQVIQLTFEEITQEVLALLKQDFLWSMDDLFPVFLFVVLRARIRNLGSEVSLIEDLMDPCVQHGEHGIMFTTLKACYYQIQHEKVT

Target ORF information:

RefSeq Version	XM_002662296.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002662296.6

ORF Insert Sequence:

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG 
AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT 
GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA 
CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT 
GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG 
TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC 
ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA 
GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC 
AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA 
TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG 
TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT 
AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG 
AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA 
TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT 
CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT 
ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT 
GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG 
AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA 
GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG 
CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT 
GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA 
TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG 
TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT 
TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GTTTTCCACC 
CATCACATTT TTCCATTGGC TACGCTGTGG GTGGAGCCAA TTTCTGAGGA GAGCACAGGC 
GTATATGGCT TAAAAGTGAC TTCGCCTGAG GAAAGCTTCG CTCTGCTGGC TTCATCACCA 
GCTGAAAAAG CAAAGTGGCT TCGTTCTATA AACCAAGCGG TAGAGCAGGC GCTCAGTGGA 
TTGGGGCATG ATGGGATACC TCCTTCTACA GGGATGACGC AGAGAGCAGA TCCACCCATC 
TCCCGGACAG CATCCTACAC CTTCTACAAA GACAGTCGGC TAAAGGATGC CAAATATGAT 
GGCCGATGGG TCTCAGGGAA ACCCCATGGG CGGGGCGTGG TCAAATGGCC AGATGGACGA 
ATGTACACGG GGACATTCAA GAACGGTTTT GAAGATGGTT TTGGAGATTA CGTTGTGTCC 
AATAAAACCC TGAACAGCTG TGATCATTAT CAAGGCCAGT GGAAAGATGG CAAGATGCAC 
GGTTTTGGAA CATTCAGATA TGCCTCTGGT GAAGTGTACG AAGGCTCATT TCAAGACAAC 
ATGCGTCATG GTCATGGGAT GCTGCGTAGT GGGAAGCTGA ATTCCACCTC TCCCAGTGTC 
TTTATCGGCC AGTGGCAGTA CGACAAAAAG TCTGGCTATG GAGTTTTTGA TGACATCACA 
CGAGGAGAGA AGTATATGGG CATGTGGCTG GATGATCAAC GGCAGGGGAA CGGAGTTGTT 
GTTACACAAT TTGGCCTATA CTATGAAGGT GCATTCAGCA ACAACAAAAT GATGGGTACT 
GGGGTGTTAC TCTCTGAAGA TGACACTACA TTTGAAGGAG AGTTTTTAGA AGACTGGACT 
CTTAATGGAA AGGGTGTGTT GACCATGCCG AATGGAGACT ATATTGAAGG CTCTTTTTGT 
GGAGTTTGGG GAACCGGGCT AAAGATGTCA GGTTCCTACT ACAAACCCAG CCTGTACGAC 
TCAGACAAGG AGAAGGGTCA TGCACTTAAA CTGGGCCGGT TAGCAGTTCA CTCTGAAGAG 
AAGTGGAAGG CTGTGTTTTT GGAGTGCTGG AACAGGCTCG GTTGTGATTC CCCTGTACAG 
GGGCAAACCA CGACAGCGTG GGACAACATT GCCATTGCAC TCACTGCTAA CCTGAGACAG 
CAGAGAGACA GTCCAGAGTT GCTGAGCCGC TCACATCACA AAACACTAGA GAGTCTTGAG 
TGCATCCCTC AACATGTGGG TCCTGTAACA TTAGAGGTGT ACCACACGAT CCGTCGGTAC 
CTGGTGAAGG CATGTGATAC TCCTCTGCAT CCTCTGGGTC GGCTGGTGGA GACGCTGGTT 
TCGGTATACC GGATGACGTA TGTTGGTGTT GGAGCAAACC GCAGGTTATT ACCGCAGGCC 
GTCAATGAGA TCACGTCCTA TCTCAGCCGT ATCTTCCAGC TCGTCAGATT TCTCTTTCCC 
GAACTGCCAG AAGAAGGAGG TTCACTAGCC GACCCTCCAA AAGAGAAGGT CGGCACAGAT 
GCTGATGGAA AGCTGCTGGA GTCTCCTAAA CCTGGGTGTG TGGTCAGCAG CTCTGCTCTC 
CTCCTGCCGG TGCTGTTGCC ACGTCTCTAT CCTCCTCTCT TCACGCTCTA CGCTCTAGAG 
AAAGAGAAAG AGGACGATGT GTACTGGGAG TGTGTGCTGC GACTCAACAA ACAGCCAGAT 
CTGGCACTGC TGGCTTTCCT CGGGGTCCAA CAGAAGTTCT GGCCTGTAAC TGTTACAATA 
CATGGAGAAA AACAACAGGT TCTCTCAAGC ACTAAAGACG CCTGTTTCGC CTCAGCAGTT 
GAGACTTTGC AGCAAATAAG CACAACGTTC ACACCCTCAG ACAAGCTTCA GGTGATCCAG 
CTGACCTTCG AGGAAATAAC CCAGGAAGTG CTCGCACTCC TGAAACAGGA TTTCTTGTGG 
TCTATGGATG ACCTGTTTCC TGTCTTCCTC TTTGTTGTTC TGCGTGCACG GATAAGGAAT 
CTGGGGTCAG AGGTTAGTCT CATTGAGGAC CTAATGGATC CATGTGTACA ACATGGAGAG 
CATGGCATCA TGTTCACCAC CCTCAAGGCG TGCTACTATC AAATCCAGCA TGAGAAGGTC 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

als2b ( XM_009302175.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300450.1 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X1, mRNA.

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CloneID	ODa49117
Clone ID Related Accession (Same CDS sequence)	XM_009302175.3 , XM_009302175.2
Accession Version	XM_009302175.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	alsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302175.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG 
AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT 
CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC 
CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA 
CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG 
GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT 
TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC 
CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG 
CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG 
CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA 
CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT 
GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT 
GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG 
CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG 
CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG 
GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA 
GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA 
GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA 
GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG 
GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA 
CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA 
AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC 
CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG 
CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC 
AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGGGTATGGT CCCCTATAAG 
AGTCTTTTTT CCACCCATCA CATTTTTCCA TTGGCTACGC TGTGGGTGGA GCCAATTTCT 
GAGGAGAGCA CAGGCGTATA TGGCTTAAAA GTGACTTCGC CTGAGGAAAG CTTCGCTCTG 
CTGGCTTCAT CACCAGCTGA AAAAGCAAAG TGGCTTCGTT CTATAAACCA AGCGGTAGAG 
CAGGCGCTCA GTGGATTGGG GCATGATGGG ATACCTCCTT CTACAGGGAT GACGCAGAGA 
GCAGATCCAC CCATCTCCCG GACAGCATCC TACACCTTCT ACAAAGACAG TCGGCTAAAG 
GATGCCAAAT ATGATGGCCG ATGGGTCTCA GGGAAACCCC ATGGGCGGGG CGTGGTCAAA 
TGGCCAGATG GACGAATGTA CACGGGGACA TTCAAGAACG GTTTTGAAGA TGGTTTTGGA 
GATTACGTTG TGTCCAATAA AACCCTGAAC AGCTGTGATC ATTATCAAGG CCAGTGGAAA 
GATGGCAAGA TGCACGGTTT TGGAACATTC AGATATGCCT CTGGTGAAGT GTACGAAGGC 
TCATTTCAAG ACAACATGCG TCATGGTCAT GGGATGCTGC GTAGTGGGAA GCTGAATTCC 
ACCTCTCCCA GTGTCTTTAT CGGCCAGTGG CAGTACGACA AAAAGTCTGG CTATGGAGTT 
TTTGATGACA TCACACGAGG AGAGAAGTAT ATGGGCATGT GGCTGGATGA TCAACGGCAG 
GGGAACGGAG TTGTTGTTAC ACAATTTGGC CTATACTATG AAGGTGCATT CAGCAACAAC 
AAAATGATGG GTACTGGGGT GTTACTCTCT GAAGATGACA CTACATTTGA AGGAGAGTTT 
TTAGAAGACT GGACTCTTAA TGGAAAGGGT GTGTTGACCA TGCCGAATGG AGACTATATT 
GAAGGCTCTT TTTGTGGAGT TTGGGGAACC GGGCTAAAGA TGTCAGGTTC CTACTACAAA 
CCCAGCCTGT ACGACTCAGA CAAGGAGAAG GGTCATGCAC TTAAACTGGG CCGGTTAGCA 
GTTCACTCTG AAGAGAAGTG GAAGGCTGTG TTTTTGGAGT GCTGGAACAG GCTCGGTTGT 
GATTCCCCTG TACAGGGGCA AACCACGACA GCGTGGGACA ACATTGCCAT TGCACTCACT 
GCTAACCTGA GACAGCAGAG AGACAGTCCA GAGTTGCTGA GCCGCTCACA TCACAAAACA 
CTAGAGAGTC TTGAGTGCAT CCCTCAACAT GTGGGTCCTG TAACATTAGA GGTGTACCAC 
ACGATCCGTC GGTACCTGGT GAAGGCATGT GATACTCCTC TGCATCCTCT GGGTCGGCTG 
GTGGAGACGC TGGTTTCGGT ATACCGGATG ACGTATGTTG GTGTTGGAGC AAACCGCAGG 
TTATTACCGC AGGCCGTCAA TGAGATCACG TCCTATCTCA GCCGTATCTT CCAGCTCGTC 
AGATTTCTCT TTCCCGAACT GCCAGAAGAA GGAGGTTCAC TAGCCGACCC TCCAAAAGAG 
AAGGTCGGCA CAGATGCTGA TGGAAAGCTG CTGGAGTCTC CTAAACCTGG GTGTGTGGTC 
AGCAGCTCTG CTCTCCTCCT GCCGGTGCTG TTGCCACGTC TCTATCCTCC TCTCTTCACG 
CTCTACGCTC TAGAGAAAGA GAAAGAGGAC GATGTGTACT GGGAGTGTGT GCTGCGACTC 
AACAAACAGC CAGATCTGGC ACTGCTGGCT TTCCTCGGGG TCCAACAGAA GTTCTGGCCT 
GTAACTGTTA CAATACATGG AGAAAAACAA CAGGTTCTCT CAAGCACTAA AGACGCCTGT 
TTCGCCTCAG CAGTTGAGAC TTTGCAGCAA ATAAGCACAA CGTTCACACC CTCAGACAAG 
CTTCAGGTGA TCCAGCTGAC CTTCGAGGAA ATAACCCAGG AAGTGCTCGC ACTCCTGAAA 
CAGGATTTCT TGTGGTCTAT GGATGACCTG TTTCCTGTCT TCCTCTTTGT TGTTCTGCGT 
GCACGGATAA GGAATCTGGG GTCAGAGGTT AGTCTCATTG AGGACCTAAT GGATCCATGT 
GTACAACATG GAGAGCATGG CATCATGTTC ACCACCCTCA AGGCGTGCTA CTATCAAATC 
CAGCATGAGA AGGTCACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300450.1
CDS	270..5390
Translation	MEKLISSGEGDSSGERGLLHTWKGYSYSTAPERVLLSRPVLQAALGARHGVLLVEGGQVYSFGELPWKQNQTSSAEPILEAVLSEQRVVFVAAGSAHSGVVTEDGGVHMWGENVHGQCGLLGLSVIPNPTPVGVLDSEATPPQTVKILEVACGDQHTLALSAKHEVWAWGSGCQLGLNTSTFPVWKPQKVDHLSGRHVLQVACGSAHSVALVRCLPPPQEPRKPPQDKCGQCHQLLYTMTDKEDHVIISDGHHCPLGVEMDSTEIKSAVEGSPDPGKPGKSSPTEPVPCMSTQISKSPTSSNISESTRPPESTLPFELQNQVPAYCQESIEAPVEDSAAHEAPAAAGEVCVEASENSIPEPDSCRSSGKSPYPDEQAVKAYLKRLSDHALAEHTIKTSSTIHSAQLSGDPANLFASEPLIPVAQSVTPMGSALNNLVVSCASAVGERVVSTYEALSLRKVIGYWVPGEGRERVEERLRLEESMQGKKSSSLGDIREEEAELSRRLSLPGLLSQDNAESEVKASSSNTTTEVSPRLLRRTSRPRMRAVPLASGGIPETDAHLPSLQTEVWSWGQGQDGQLGHGDLLPRLQPDCVKSLNGKEVLKVAAGAHHSLALTAQSQVFSWGCNTSGQLGHMESPTTFPQLTKLSDGIRVWDIGAGLQHTLLLADGDCIQPILYYSGQQVKEVPEPTEEEEQTAEYTQQPVLLPFCMNLGYVSSVYAGGRTCAALTDCNVMGFIASLHELAAAERKYYCKLSNIKSLLLQPLLKLDSLSSALGQSSAGLLQNLIGRMSRLSQLTGQNSASLTSFLQRSRDIRGLVILEDAHLFLDTYSEYSSAVGDLLVMGGFQALVKPCHDVFGKGAEVVQRLSECSEEGLTLADVLAHLFYLPIKHLHEYGRLLLKLATCYEVSSVDYQKLQDACSKFESMALHLKRRRKEAEYTLHFWKSFPGKMTDSLRKPSRRLICESSNKALTLQNAGRFSVNWFILFNDALVHAQGMVPYKSLFSTHHIFPLATLWVEPISEESTGVYGLKVTSPEESFALLASSPAEKAKWLRSINQAVEQALSGLGHDGIPPSTGMTQRADPPISRTASYTFYKDSRLKDAKYDGRWVSGKPHGRGVVKWPDGRMYTGTFKNGFEDGFGDYVVSNKTLNSCDHYQGQWKDGKMHGFGTFRYASGEVYEGSFQDNMRHGHGMLRSGKLNSTSPSVFIGQWQYDKKSGYGVFDDITRGEKYMGMWLDDQRQGNGVVVTQFGLYYEGAFSNNKMMGTGVLLSEDDTTFEGEFLEDWTLNGKGVLTMPNGDYIEGSFCGVWGTGLKMSGSYYKPSLYDSDKEKGHALKLGRLAVHSEEKWKAVFLECWNRLGCDSPVQGQTTTAWDNIAIALTANLRQQRDSPELLSRSHHKTLESLECIPQHVGPVTLEVYHTIRRYLVKACDTPLHPLGRLVETLVSVYRMTYVGVGANRRLLPQAVNEITSYLSRIFQLVRFLFPELPEEGGSLADPPKEKVGTDADGKLLESPKPGCVVSSSALLLPVLLPRLYPPLFTLYALEKEKEDDVYWECVLRLNKQPDLALLAFLGVQQKFWPVTVTIHGEKQQVLSSTKDACFASAVETLQQISTTFTPSDKLQVIQLTFEEITQEVLALLKQDFLWSMDDLFPVFLFVVLRARIRNLGSEVSLIEDLMDPCVQHGEHGIMFTTLKACYYQIQHEKVT

Target ORF information:

RefSeq Version	XM_009302175.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302175.3

ORF Insert Sequence:

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG 
AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT 
CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC 
CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA 
CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG 
GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT 
TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC 
CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG 
CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG 
CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA 
CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT 
GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT 
GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG 
CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG 
CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG 
GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA 
GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA 
GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA 
GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG 
GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA 
CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA 
AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC 
CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG 
CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC 
AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGGGTATGGT CCCCTATAAG 
AGTCTTTTTT CCACCCATCA CATTTTTCCA TTGGCTACGC TGTGGGTGGA GCCAATTTCT 
GAGGAGAGCA CAGGCGTATA TGGCTTAAAA GTGACTTCGC CTGAGGAAAG CTTCGCTCTG 
CTGGCTTCAT CACCAGCTGA AAAAGCAAAG TGGCTTCGTT CTATAAACCA AGCGGTAGAG 
CAGGCGCTCA GTGGATTGGG GCATGATGGG ATACCTCCTT CTACAGGGAT GACGCAGAGA 
GCAGATCCAC CCATCTCCCG GACAGCATCC TACACCTTCT ACAAAGACAG TCGGCTAAAG 
GATGCCAAAT ATGATGGCCG ATGGGTCTCA GGGAAACCCC ATGGGCGGGG CGTGGTCAAA 
TGGCCAGATG GACGAATGTA CACGGGGACA TTCAAGAACG GTTTTGAAGA TGGTTTTGGA 
GATTACGTTG TGTCCAATAA AACCCTGAAC AGCTGTGATC ATTATCAAGG CCAGTGGAAA 
GATGGCAAGA TGCACGGTTT TGGAACATTC AGATATGCCT CTGGTGAAGT GTACGAAGGC 
TCATTTCAAG ACAACATGCG TCATGGTCAT GGGATGCTGC GTAGTGGGAA GCTGAATTCC 
ACCTCTCCCA GTGTCTTTAT CGGCCAGTGG CAGTACGACA AAAAGTCTGG CTATGGAGTT 
TTTGATGACA TCACACGAGG AGAGAAGTAT ATGGGCATGT GGCTGGATGA TCAACGGCAG 
GGGAACGGAG TTGTTGTTAC ACAATTTGGC CTATACTATG AAGGTGCATT CAGCAACAAC 
AAAATGATGG GTACTGGGGT GTTACTCTCT GAAGATGACA CTACATTTGA AGGAGAGTTT 
TTAGAAGACT GGACTCTTAA TGGAAAGGGT GTGTTGACCA TGCCGAATGG AGACTATATT 
GAAGGCTCTT TTTGTGGAGT TTGGGGAACC GGGCTAAAGA TGTCAGGTTC CTACTACAAA 
CCCAGCCTGT ACGACTCAGA CAAGGAGAAG GGTCATGCAC TTAAACTGGG CCGGTTAGCA 
GTTCACTCTG AAGAGAAGTG GAAGGCTGTG TTTTTGGAGT GCTGGAACAG GCTCGGTTGT 
GATTCCCCTG TACAGGGGCA AACCACGACA GCGTGGGACA ACATTGCCAT TGCACTCACT 
GCTAACCTGA GACAGCAGAG AGACAGTCCA GAGTTGCTGA GCCGCTCACA TCACAAAACA 
CTAGAGAGTC TTGAGTGCAT CCCTCAACAT GTGGGTCCTG TAACATTAGA GGTGTACCAC 
ACGATCCGTC GGTACCTGGT GAAGGCATGT GATACTCCTC TGCATCCTCT GGGTCGGCTG 
GTGGAGACGC TGGTTTCGGT ATACCGGATG ACGTATGTTG GTGTTGGAGC AAACCGCAGG 
TTATTACCGC AGGCCGTCAA TGAGATCACG TCCTATCTCA GCCGTATCTT CCAGCTCGTC 
AGATTTCTCT TTCCCGAACT GCCAGAAGAA GGAGGTTCAC TAGCCGACCC TCCAAAAGAG 
AAGGTCGGCA CAGATGCTGA TGGAAAGCTG CTGGAGTCTC CTAAACCTGG GTGTGTGGTC 
AGCAGCTCTG CTCTCCTCCT GCCGGTGCTG TTGCCACGTC TCTATCCTCC TCTCTTCACG 
CTCTACGCTC TAGAGAAAGA GAAAGAGGAC GATGTGTACT GGGAGTGTGT GCTGCGACTC 
AACAAACAGC CAGATCTGGC ACTGCTGGCT TTCCTCGGGG TCCAACAGAA GTTCTGGCCT 
GTAACTGTTA CAATACATGG AGAAAAACAA CAGGTTCTCT CAAGCACTAA AGACGCCTGT 
TTCGCCTCAG CAGTTGAGAC TTTGCAGCAA ATAAGCACAA CGTTCACACC CTCAGACAAG 
CTTCAGGTGA TCCAGCTGAC CTTCGAGGAA ATAACCCAGG AAGTGCTCGC ACTCCTGAAA 
CAGGATTTCT TGTGGTCTAT GGATGACCTG TTTCCTGTCT TCCTCTTTGT TGTTCTGCGT 
GCACGGATAA GGAATCTGGG GTCAGAGGTT AGTCTCATTG AGGACCTAAT GGATCCATGT 
GTACAACATG GAGAGCATGG CATCATGTTC ACCACCCTCA AGGCGTGCTA CTATCAAATC 
CAGCATGAGA AGGTCACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

als2b ( XM_009302175.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300450.1 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X1, mRNA.

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CloneID	ODa49117
Clone ID Related Accession (Same CDS sequence)	XM_009302175.3 , XM_009302175.2
Accession Version	XM_009302175.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	alsin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302175.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG 
AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT 
CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC 
CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA 
CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG 
GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT 
TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC 
CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG 
CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG 
CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA 
CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT 
GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT 
GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG 
CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG 
CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG 
GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA 
GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA 
GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA 
GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG 
GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA 
CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA 
AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC 
CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG 
CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC 
AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGGGTATGGT CCCCTATAAG 
AGTCTTTTTT CCACCCATCA CATTTTTCCA TTGGCTACGC TGTGGGTGGA GCCAATTTCT 
GAGGAGAGCA CAGGCGTATA TGGCTTAAAA GTGACTTCGC CTGAGGAAAG CTTCGCTCTG 
CTGGCTTCAT CACCAGCTGA AAAAGCAAAG TGGCTTCGTT CTATAAACCA AGCGGTAGAG 
CAGGCGCTCA GTGGATTGGG GCATGATGGG ATACCTCCTT CTACAGGGAT GACGCAGAGA 
GCAGATCCAC CCATCTCCCG GACAGCATCC TACACCTTCT ACAAAGACAG TCGGCTAAAG 
GATGCCAAAT ATGATGGCCG ATGGGTCTCA GGGAAACCCC ATGGGCGGGG CGTGGTCAAA 
TGGCCAGATG GACGAATGTA CACGGGGACA TTCAAGAACG GTTTTGAAGA TGGTTTTGGA 
GATTACGTTG TGTCCAATAA AACCCTGAAC AGCTGTGATC ATTATCAAGG CCAGTGGAAA 
GATGGCAAGA TGCACGGTTT TGGAACATTC AGATATGCCT CTGGTGAAGT GTACGAAGGC 
TCATTTCAAG ACAACATGCG TCATGGTCAT GGGATGCTGC GTAGTGGGAA GCTGAATTCC 
ACCTCTCCCA GTGTCTTTAT CGGCCAGTGG CAGTACGACA AAAAGTCTGG CTATGGAGTT 
TTTGATGACA TCACACGAGG AGAGAAGTAT ATGGGCATGT GGCTGGATGA TCAACGGCAG 
GGGAACGGAG TTGTTGTTAC ACAATTTGGC CTATACTATG AAGGTGCATT CAGCAACAAC 
AAAATGATGG GTACTGGGGT GTTACTCTCT GAAGATGACA CTACATTTGA AGGAGAGTTT 
TTAGAAGACT GGACTCTTAA TGGAAAGGGT GTGTTGACCA TGCCGAATGG AGACTATATT 
GAAGGCTCTT TTTGTGGAGT TTGGGGAACC GGGCTAAAGA TGTCAGGTTC CTACTACAAA 
CCCAGCCTGT ACGACTCAGA CAAGGAGAAG GGTCATGCAC TTAAACTGGG CCGGTTAGCA 
GTTCACTCTG AAGAGAAGTG GAAGGCTGTG TTTTTGGAGT GCTGGAACAG GCTCGGTTGT 
GATTCCCCTG TACAGGGGCA AACCACGACA GCGTGGGACA ACATTGCCAT TGCACTCACT 
GCTAACCTGA GACAGCAGAG AGACAGTCCA GAGTTGCTGA GCCGCTCACA TCACAAAACA 
CTAGAGAGTC TTGAGTGCAT CCCTCAACAT GTGGGTCCTG TAACATTAGA GGTGTACCAC 
ACGATCCGTC GGTACCTGGT GAAGGCATGT GATACTCCTC TGCATCCTCT GGGTCGGCTG 
GTGGAGACGC TGGTTTCGGT ATACCGGATG ACGTATGTTG GTGTTGGAGC AAACCGCAGG 
TTATTACCGC AGGCCGTCAA TGAGATCACG TCCTATCTCA GCCGTATCTT CCAGCTCGTC 
AGATTTCTCT TTCCCGAACT GCCAGAAGAA GGAGGTTCAC TAGCCGACCC TCCAAAAGAG 
AAGGTCGGCA CAGATGCTGA TGGAAAGCTG CTGGAGTCTC CTAAACCTGG GTGTGTGGTC 
AGCAGCTCTG CTCTCCTCCT GCCGGTGCTG TTGCCACGTC TCTATCCTCC TCTCTTCACG 
CTCTACGCTC TAGAGAAAGA GAAAGAGGAC GATGTGTACT GGGAGTGTGT GCTGCGACTC 
AACAAACAGC CAGATCTGGC ACTGCTGGCT TTCCTCGGGG TCCAACAGAA GTTCTGGCCT 
GTAACTGTTA CAATACATGG AGAAAAACAA CAGGTTCTCT CAAGCACTAA AGACGCCTGT 
TTCGCCTCAG CAGTTGAGAC TTTGCAGCAA ATAAGCACAA CGTTCACACC CTCAGACAAG 
CTTCAGGTGA TCCAGCTGAC CTTCGAGGAA ATAACCCAGG AAGTGCTCGC ACTCCTGAAA 
CAGGATTTCT TGTGGTCTAT GGATGACCTG TTTCCTGTCT TCCTCTTTGT TGTTCTGCGT 
GCACGGATAA GGAATCTGGG GTCAGAGGTT AGTCTCATTG AGGACCTAAT GGATCCATGT 
GTACAACATG GAGAGCATGG CATCATGTTC ACCACCCTCA AGGCGTGCTA CTATCAAATC 
CAGCATGAGA AGGTCACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300450.1
CDS	250..5370
Translation	MEKLISSGEGDSSGERGLLHTWKGYSYSTAPERVLLSRPVLQAALGARHGVLLVEGGQVYSFGELPWKQNQTSSAEPILEAVLSEQRVVFVAAGSAHSGVVTEDGGVHMWGENVHGQCGLLGLSVIPNPTPVGVLDSEATPPQTVKILEVACGDQHTLALSAKHEVWAWGSGCQLGLNTSTFPVWKPQKVDHLSGRHVLQVACGSAHSVALVRCLPPPQEPRKPPQDKCGQCHQLLYTMTDKEDHVIISDGHHCPLGVEMDSTEIKSAVEGSPDPGKPGKSSPTEPVPCMSTQISKSPTSSNISESTRPPESTLPFELQNQVPAYCQESIEAPVEDSAAHEAPAAAGEVCVEASENSIPEPDSCRSSGKSPYPDEQAVKAYLKRLSDHALAEHTIKTSSTIHSAQLSGDPANLFASEPLIPVAQSVTPMGSALNNLVVSCASAVGERVVSTYEALSLRKVIGYWVPGEGRERVEERLRLEESMQGKKSSSLGDIREEEAELSRRLSLPGLLSQDNAESEVKASSSNTTTEVSPRLLRRTSRPRMRAVPLASGGIPETDAHLPSLQTEVWSWGQGQDGQLGHGDLLPRLQPDCVKSLNGKEVLKVAAGAHHSLALTAQSQVFSWGCNTSGQLGHMESPTTFPQLTKLSDGIRVWDIGAGLQHTLLLADGDCIQPILYYSGQQVKEVPEPTEEEEQTAEYTQQPVLLPFCMNLGYVSSVYAGGRTCAALTDCNVMGFIASLHELAAAERKYYCKLSNIKSLLLQPLLKLDSLSSALGQSSAGLLQNLIGRMSRLSQLTGQNSASLTSFLQRSRDIRGLVILEDAHLFLDTYSEYSSAVGDLLVMGGFQALVKPCHDVFGKGAEVVQRLSECSEEGLTLADVLAHLFYLPIKHLHEYGRLLLKLATCYEVSSVDYQKLQDACSKFESMALHLKRRRKEAEYTLHFWKSFPGKMTDSLRKPSRRLICESSNKALTLQNAGRFSVNWFILFNDALVHAQGMVPYKSLFSTHHIFPLATLWVEPISEESTGVYGLKVTSPEESFALLASSPAEKAKWLRSINQAVEQALSGLGHDGIPPSTGMTQRADPPISRTASYTFYKDSRLKDAKYDGRWVSGKPHGRGVVKWPDGRMYTGTFKNGFEDGFGDYVVSNKTLNSCDHYQGQWKDGKMHGFGTFRYASGEVYEGSFQDNMRHGHGMLRSGKLNSTSPSVFIGQWQYDKKSGYGVFDDITRGEKYMGMWLDDQRQGNGVVVTQFGLYYEGAFSNNKMMGTGVLLSEDDTTFEGEFLEDWTLNGKGVLTMPNGDYIEGSFCGVWGTGLKMSGSYYKPSLYDSDKEKGHALKLGRLAVHSEEKWKAVFLECWNRLGCDSPVQGQTTTAWDNIAIALTANLRQQRDSPELLSRSHHKTLESLECIPQHVGPVTLEVYHTIRRYLVKACDTPLHPLGRLVETLVSVYRMTYVGVGANRRLLPQAVNEITSYLSRIFQLVRFLFPELPEEGGSLADPPKEKVGTDADGKLLESPKPGCVVSSSALLLPVLLPRLYPPLFTLYALEKEKEDDVYWECVLRLNKQPDLALLAFLGVQQKFWPVTVTIHGEKQQVLSSTKDACFASAVETLQQISTTFTPSDKLQVIQLTFEEITQEVLALLKQDFLWSMDDLFPVFLFVVLRARIRNLGSEVSLIEDLMDPCVQHGEHGIMFTTLKACYYQIQHEKVT

Target ORF information:

RefSeq Version	XM_009302175.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302175.2

ORF Insert Sequence:

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG ACAATGCAGA GTCTGAGGTG 
AAGGCGTCGT CATCCAACAC CACAACTGAA GTATCTCCTC GCCTCCTACG GAGAACAAGT 
CGCCCCCGTA TGCGGGCTGT CCCGCTCGCT TCAGGAGGTA TACCTGAGAC TGATGCCCAC 
CTGCCTTCCT TACAGACGGA AGTGTGGAGC TGGGGACAAG GCCAGGATGG ACAACTTGGA 
CATGGAGACC TCCTTCCCAG ACTGCAGCCT GATTGTGTAA AGAGTCTAAA TGGTAAGGAG 
GTGCTGAAAG TGGCTGCAGG GGCTCATCAC TCTCTCGCTC TGACAGCACA GTCACAGGTT 
TTCTCCTGGG GTTGTAACAC TTCTGGACAG CTGGGACACA TGGAATCGCC CACCACCTTC 
CCTCAATTGA CCAAGCTGTC AGATGGGATT CGTGTGTGGG ATATTGGGGC AGGTCTGCAG 
CACACTCTCT TATTGGCTGA CGGAGACTGT ATTCAGCCAA TCCTGTACTA CAGCGGACAG 
CAGGTGAAGG AAGTGCCGGA GCCAACAGAG GAAGAGGAGC AGACTGCAGA ATACACGCAA 
CAACCTGTGC TGCTGCCCTT CTGTATGAAC TTGGGCTATG TAAGCAGTGT GTATGCAGGT 
GGACGGACGT GTGCAGCGCT GACGGATTGT AATGTCATGG GTTTTATTGC TAGTCTGCAT 
GAGCTGGCGG CTGCAGAGAG AAAATATTAC TGCAAACTCA GCAATATCAA GAGTCTTCTG 
CTCCAGCCAT TGCTCAAGCT GGATTCTCTA AGCTCTGCCC TGGGTCAGTC ATCTGCAGGG 
CTGTTGCAGA ATCTGATTGG TCGAATGAGT CGACTGTCTC AGCTTACAGG TCAAAACTCG 
GCCTCTCTGA CCTCGTTCCT GCAGCGTTCA CGAGACATCC GAGGTCTTGT TATTCTTGAA 
GATGCACATC TGTTCCTGGA CACATATTCA GAGTACTCTT CTGCAGTTGG TGATCTTTTA 
GTCATGGGAG GATTTCAAGC CCTTGTCAAA CCTTGCCATG ATGTGTTTGG GAAAGGAGCA 
GAGGTTGTTC AGAGGTTGTC TGAATGTTCT GAAGAAGGTT TGACCTTGGC AGATGTTCTG 
GCGCATCTCT TTTACCTGCC CATAAAACAC CTGCATGAAT ATGGACGACT GCTGCTTAAA 
CTGGCTACCT GCTATGAGGT GAGTTCTGTG GACTATCAGA AGCTGCAGGA TGCCTGCTCA 
AAGTTCGAAT CCATGGCTCT TCATCTAAAG AGGAGGAGAA AGGAAGCTGA ATACACGCTC 
CACTTCTGGA AGAGTTTTCC TGGAAAGATG ACCGACTCCC TGCGTAAACC GTCTCGTCGG 
CTGATCTGTG AAAGCAGTAA TAAGGCTTTG ACTCTTCAAA ACGCAGGACG TTTTTCCGTC 
AACTGGTTCA TCCTCTTCAA CGACGCACTC GTTCATGCAC AGGGTATGGT CCCCTATAAG 
AGTCTTTTTT CCACCCATCA CATTTTTCCA TTGGCTACGC TGTGGGTGGA GCCAATTTCT 
GAGGAGAGCA CAGGCGTATA TGGCTTAAAA GTGACTTCGC CTGAGGAAAG CTTCGCTCTG 
CTGGCTTCAT CACCAGCTGA AAAAGCAAAG TGGCTTCGTT CTATAAACCA AGCGGTAGAG 
CAGGCGCTCA GTGGATTGGG GCATGATGGG ATACCTCCTT CTACAGGGAT GACGCAGAGA 
GCAGATCCAC CCATCTCCCG GACAGCATCC TACACCTTCT ACAAAGACAG TCGGCTAAAG 
GATGCCAAAT ATGATGGCCG ATGGGTCTCA GGGAAACCCC ATGGGCGGGG CGTGGTCAAA 
TGGCCAGATG GACGAATGTA CACGGGGACA TTCAAGAACG GTTTTGAAGA TGGTTTTGGA 
GATTACGTTG TGTCCAATAA AACCCTGAAC AGCTGTGATC ATTATCAAGG CCAGTGGAAA 
GATGGCAAGA TGCACGGTTT TGGAACATTC AGATATGCCT CTGGTGAAGT GTACGAAGGC 
TCATTTCAAG ACAACATGCG TCATGGTCAT GGGATGCTGC GTAGTGGGAA GCTGAATTCC 
ACCTCTCCCA GTGTCTTTAT CGGCCAGTGG CAGTACGACA AAAAGTCTGG CTATGGAGTT 
TTTGATGACA TCACACGAGG AGAGAAGTAT ATGGGCATGT GGCTGGATGA TCAACGGCAG 
GGGAACGGAG TTGTTGTTAC ACAATTTGGC CTATACTATG AAGGTGCATT CAGCAACAAC 
AAAATGATGG GTACTGGGGT GTTACTCTCT GAAGATGACA CTACATTTGA AGGAGAGTTT 
TTAGAAGACT GGACTCTTAA TGGAAAGGGT GTGTTGACCA TGCCGAATGG AGACTATATT 
GAAGGCTCTT TTTGTGGAGT TTGGGGAACC GGGCTAAAGA TGTCAGGTTC CTACTACAAA 
CCCAGCCTGT ACGACTCAGA CAAGGAGAAG GGTCATGCAC TTAAACTGGG CCGGTTAGCA 
GTTCACTCTG AAGAGAAGTG GAAGGCTGTG TTTTTGGAGT GCTGGAACAG GCTCGGTTGT 
GATTCCCCTG TACAGGGGCA AACCACGACA GCGTGGGACA ACATTGCCAT TGCACTCACT 
GCTAACCTGA GACAGCAGAG AGACAGTCCA GAGTTGCTGA GCCGCTCACA TCACAAAACA 
CTAGAGAGTC TTGAGTGCAT CCCTCAACAT GTGGGTCCTG TAACATTAGA GGTGTACCAC 
ACGATCCGTC GGTACCTGGT GAAGGCATGT GATACTCCTC TGCATCCTCT GGGTCGGCTG 
GTGGAGACGC TGGTTTCGGT ATACCGGATG ACGTATGTTG GTGTTGGAGC AAACCGCAGG 
TTATTACCGC AGGCCGTCAA TGAGATCACG TCCTATCTCA GCCGTATCTT CCAGCTCGTC 
AGATTTCTCT TTCCCGAACT GCCAGAAGAA GGAGGTTCAC TAGCCGACCC TCCAAAAGAG 
AAGGTCGGCA CAGATGCTGA TGGAAAGCTG CTGGAGTCTC CTAAACCTGG GTGTGTGGTC 
AGCAGCTCTG CTCTCCTCCT GCCGGTGCTG TTGCCACGTC TCTATCCTCC TCTCTTCACG 
CTCTACGCTC TAGAGAAAGA GAAAGAGGAC GATGTGTACT GGGAGTGTGT GCTGCGACTC 
AACAAACAGC CAGATCTGGC ACTGCTGGCT TTCCTCGGGG TCCAACAGAA GTTCTGGCCT 
GTAACTGTTA CAATACATGG AGAAAAACAA CAGGTTCTCT CAAGCACTAA AGACGCCTGT 
TTCGCCTCAG CAGTTGAGAC TTTGCAGCAA ATAAGCACAA CGTTCACACC CTCAGACAAG 
CTTCAGGTGA TCCAGCTGAC CTTCGAGGAA ATAACCCAGG AAGTGCTCGC ACTCCTGAAA 
CAGGATTTCT TGTGGTCTAT GGATGACCTG TTTCCTGTCT TCCTCTTTGT TGTTCTGCGT 
GCACGGATAA GGAATCTGGG GTCAGAGGTT AGTCTCATTG AGGACCTAAT GGATCCATGT 
GTACAACATG GAGAGCATGG CATCATGTTC ACCACCCTCA AGGCGTGCTA CTATCAAATC 
CAGCATGAGA AGGTCACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

als2b ( XM_009302176.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300451.1 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X2, mRNA.

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CloneID	ODa49118
Clone ID Related Accession (Same CDS sequence)	XM_009302176.2 , XM_009302176.3
Accession Version	XM_009302176.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	alsin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG GTACACACGC TCTCCTTCTC 
TTACACCCAG TGTCCCGTTT CCTATCTCCT CGCCTCCTAC GGAGAACAAG TCGCCCCCGT 
ATGCGGGCTG TCCCGCTCGC TTCAGGAGGT ATACCTGAGA CTGATGCCCA CCTGCCTTCC 
TTACAGACGG AAGTGTGGAG CTGGGGACAA GGCCAGGATG GACAACTTGG ACATGGAGAC 
CTCCTTCCCA GACTGCAGCC TGATTGTGTA AAGAGTCTAA ATGGTAAGGA GGTGCTGAAA 
GTGGCTGCAG GGGCTCATCA CTCTCTCGCT CTGACAGCAC AGTCACAGGT TTTCTCCTGG 
GGTTGTAACA CTTCTGGACA GCTGGGACAC ATGGAATCGC CCACCACCTT CCCTCAATTG 
ACCAAGCTGT CAGATGGGAT TCGTGTGTGG GATATTGGGG CAGGTCTGCA GCACACTCTC 
TTATTGGCTG ACGGAGACTG TATTCAGCCA ATCCTGTACT ACAGCGGACA GCAGGTGAAG 
GAAGTGCCGG AGCCAACAGA GGAAGAGGAG CAGACTGCAG AATACACGCA ACAACCTGTG 
CTGCTGCCCT TCTGTATGAA CTTGGGCTAT GTAAGCAGTG TGTATGCAGG TGGACGGACG 
TGTGCAGCGC TGACGGATTG TAATGTCATG GGTTTTATTG CTAGTCTGCA TGAGCTGGCG 
GCTGCAGAGA GAAAATATTA CTGCAAACTC AGCAATATCA AGAGTCTTCT GCTCCAGCCA 
TTGCTCAAGC TGGATTCTCT AAGCTCTGCC CTGGGTCAGT CATCTGCAGG GCTGTTGCAG 
AATCTGATTG GTCGAATGAG TCGACTGTCT CAGCTTACAG GTCAAAACTC GGCCTCTCTG 
ACCTCGTTCC TGCAGCGTTC ACGAGACATC CGAGGTCTTG TTATTCTTGA AGATGCACAT 
CTGTTCCTGG ACACATATTC AGAGTACTCT TCTGCAGTTG GTGATCTTTT AGTCATGGGA 
GGATTTCAAG CCCTTGTCAA ACCTTGCCAT GATGTGTTTG GGAAAGGAGC AGAGGTTGTT 
CAGAGGTTGT CTGAATGTTC TGAAGAAGGT TTGACCTTGG CAGATGTTCT GGCGCATCTC 
TTTTACCTGC CCATAAAACA CCTGCATGAA TATGGACGAC TGCTGCTTAA ACTGGCTACC 
TGCTATGAGG TGAGTTCTGT GGACTATCAG AAGCTGCAGG ATGCCTGCTC AAAGTTCGAA 
TCCATGGCTC TTCATCTAAA GAGGAGGAGA AAGGAAGCTG AATACACGCT CCACTTCTGG 
AAGAGTTTTC CTGGAAAGAT GACCGACTCC CTGCGTAAAC CGTCTCGTCG GCTGATCTGT 
GAAAGCAGTA ATAAGGCTTT GACTCTTCAA AACGCAGGAC GTTTTTCCGT CAACTGGTTC 
ATCCTCTTCA ACGACGCACT CGTTCATGCA CAGGGTATGG TCCCCTATAA GAGTCTTTTT 
TCCACCCATC ACATTTTTCC ATTGGCTACG CTGTGGGTGG AGCCAATTTC TGAGGAGAGC 
ACAGGCGTAT ATGGCTTAAA AGTGACTTCG CCTGAGGAAA GCTTCGCTCT GCTGGCTTCA 
TCACCAGCTG AAAAAGCAAA GTGGCTTCGT TCTATAAACC AAGCGGTAGA GCAGGCGCTC 
AGTGGATTGG GGCATGATGG GATACCTCCT TCTACAGGGA TGACGCAGAG AGCAGATCCA 
CCCATCTCCC GGACAGCATC CTACACCTTC TACAAAGACA GTCGGCTAAA GGATGCCAAA 
TATGATGGCC GATGGGTCTC AGGGAAACCC CATGGGCGGG GCGTGGTCAA ATGGCCAGAT 
GGACGAATGT ACACGGGGAC ATTCAAGAAC GGTTTTGAAG ATGGTTTTGG AGATTACGTT 
GTGTCCAATA AAACCCTGAA CAGCTGTGAT CATTATCAAG GCCAGTGGAA AGATGGCAAG 
ATGCACGGTT TTGGAACATT CAGATATGCC TCTGGTGAAG TGTACGAAGG CTCATTTCAA 
GACAACATGC GTCATGGTCA TGGGATGCTG CGTAGTGGGA AGCTGAATTC CACCTCTCCC 
AGTGTCTTTA TCGGCCAGTG GCAGTACGAC AAAAAGTCTG GCTATGGAGT TTTTGATGAC 
ATCACACGAG GAGAGAAGTA TATGGGCATG TGGCTGGATG ATCAACGGCA GGGGAACGGA 
GTTGTTGTTA CACAATTTGG CCTATACTAT GAAGGTGCAT TCAGCAACAA CAAAATGATG 
GGTACTGGGG TGTTACTCTC TGAAGATGAC ACTACATTTG AAGGAGAGTT TTTAGAAGAC 
TGGACTCTTA ATGGAAAGGG TGTGTTGACC ATGCCGAATG GAGACTATAT TGAAGGCTCT 
TTTTGTGGAG TTTGGGGAAC CGGGCTAAAG ATGTCAGGTT CCTACTACAA ACCCAGCCTG 
TACGACTCAG ACAAGGAGAA GGGTCATGCA CTTAAACTGG GCCGGTTAGC AGTTCACTCT 
GAAGAGAAGT GGAAGGCTGT GTTTTTGGAG TGCTGGAACA GGCTCGGTTG TGATTCCCCT 
GTACAGGGGC AAACCACGAC AGCGTGGGAC AACATTGCCA TTGCACTCAC TGCTAACCTG 
AGACAGCAGA GAGACAGTCC AGAGTTGCTG AGCCGCTCAC ATCACAAAAC ACTAGAGAGT 
CTTGAGTGCA TCCCTCAACA TGTGGGTCCT GTAACATTAG AGGTGTACCA CACGATCCGT 
CGGTACCTGG TGAAGGCATG TGATACTCCT CTGCATCCTC TGGGTCGGCT GGTGGAGACG 
CTGGTTTCGG TATACCGGAT GACGTATGTT GGTGTTGGAG CAAACCGCAG GTTATTACCG 
CAGGCCGTCA ATGAGATCAC GTCCTATCTC AGCCGTATCT TCCAGCTCGT CAGATTTCTC 
TTTCCCGAAC TGCCAGAAGA AGGAGGTTCA CTAGCCGACC CTCCAAAAGA GAAGGTCGGC 
ACAGATGCTG ATGGAAAGCT GCTGGAGTCT CCTAAACCTG GGTGTGTGGT CAGCAGCTCT 
GCTCTCCTCC TGCCGGTGCT GTTGCCACGT CTCTATCCTC CTCTCTTCAC GCTCTACGCT 
CTAGAGAAAG AGAAAGAGGA CGATGTGTAC TGGGAGTGTG TGCTGCGACT CAACAAACAG 
CCAGATCTGG CACTGCTGGC TTTCCTCGGG GTCCAACAGA AGTTCTGGCC TGTAACTGTT 
ACAATACATG GAGAAAAACA ACAGGTTCTC TCAAGCACTA AAGACGCCTG TTTCGCCTCA 
GCAGTTGAGA CTTTGCAGCA AATAAGCACA ACGTTCACAC CCTCAGACAA GCTTCAGGTG 
ATCCAGCTGA CCTTCGAGGA AATAACCCAG GAAGTGCTCG CACTCCTGAA ACAGGATTTC 
TTGTGGTCTA TGGATGACCT GTTTCCTGTC TTCCTCTTTG TTGTTCTGCG TGCACGGATA 
AGGAATCTGG GGTCAGAGGT TAGTCTCATT GAGGACCTAA TGGATCCATG TGTACAACAT 
GGAGAGCATG GCATCATGTT CACCACCCTC AAGGCGTGCT ACTATCAAAT CCAGCATGAG 
AAGGTCACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300451.1
CDS	250..5361
Translation	MEKLISSGEGDSSGERGLLHTWKGYSYSTAPERVLLSRPVLQAALGARHGVLLVEGGQVYSFGELPWKQNQTSSAEPILEAVLSEQRVVFVAAGSAHSGVVTEDGGVHMWGENVHGQCGLLGLSVIPNPTPVGVLDSEATPPQTVKILEVACGDQHTLALSAKHEVWAWGSGCQLGLNTSTFPVWKPQKVDHLSGRHVLQVACGSAHSVALVRCLPPPQEPRKPPQDKCGQCHQLLYTMTDKEDHVIISDGHHCPLGVEMDSTEIKSAVEGSPDPGKPGKSSPTEPVPCMSTQISKSPTSSNISESTRPPESTLPFELQNQVPAYCQESIEAPVEDSAAHEAPAAAGEVCVEASENSIPEPDSCRSSGKSPYPDEQAVKAYLKRLSDHALAEHTIKTSSTIHSAQLSGDPANLFASEPLIPVAQSVTPMGSALNNLVVSCASAVGERVVSTYEALSLRKVIGYWVPGEGRERVEERLRLEESMQGKKSSSLGDIREEEAELSRRLSLPGLLSQGTHALLLLHPVSRFLSPRLLRRTSRPRMRAVPLASGGIPETDAHLPSLQTEVWSWGQGQDGQLGHGDLLPRLQPDCVKSLNGKEVLKVAAGAHHSLALTAQSQVFSWGCNTSGQLGHMESPTTFPQLTKLSDGIRVWDIGAGLQHTLLLADGDCIQPILYYSGQQVKEVPEPTEEEEQTAEYTQQPVLLPFCMNLGYVSSVYAGGRTCAALTDCNVMGFIASLHELAAAERKYYCKLSNIKSLLLQPLLKLDSLSSALGQSSAGLLQNLIGRMSRLSQLTGQNSASLTSFLQRSRDIRGLVILEDAHLFLDTYSEYSSAVGDLLVMGGFQALVKPCHDVFGKGAEVVQRLSECSEEGLTLADVLAHLFYLPIKHLHEYGRLLLKLATCYEVSSVDYQKLQDACSKFESMALHLKRRRKEAEYTLHFWKSFPGKMTDSLRKPSRRLICESSNKALTLQNAGRFSVNWFILFNDALVHAQGMVPYKSLFSTHHIFPLATLWVEPISEESTGVYGLKVTSPEESFALLASSPAEKAKWLRSINQAVEQALSGLGHDGIPPSTGMTQRADPPISRTASYTFYKDSRLKDAKYDGRWVSGKPHGRGVVKWPDGRMYTGTFKNGFEDGFGDYVVSNKTLNSCDHYQGQWKDGKMHGFGTFRYASGEVYEGSFQDNMRHGHGMLRSGKLNSTSPSVFIGQWQYDKKSGYGVFDDITRGEKYMGMWLDDQRQGNGVVVTQFGLYYEGAFSNNKMMGTGVLLSEDDTTFEGEFLEDWTLNGKGVLTMPNGDYIEGSFCGVWGTGLKMSGSYYKPSLYDSDKEKGHALKLGRLAVHSEEKWKAVFLECWNRLGCDSPVQGQTTTAWDNIAIALTANLRQQRDSPELLSRSHHKTLESLECIPQHVGPVTLEVYHTIRRYLVKACDTPLHPLGRLVETLVSVYRMTYVGVGANRRLLPQAVNEITSYLSRIFQLVRFLFPELPEEGGSLADPPKEKVGTDADGKLLESPKPGCVVSSSALLLPVLLPRLYPPLFTLYALEKEKEDDVYWECVLRLNKQPDLALLAFLGVQQKFWPVTVTIHGEKQQVLSSTKDACFASAVETLQQISTTFTPSDKLQVIQLTFEEITQEVLALLKQDFLWSMDDLFPVFLFVVLRARIRNLGSEVSLIEDLMDPCVQHGEHGIMFTTLKACYYQIQHEKVT

Target ORF information:

RefSeq Version	XM_009302176.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302176.2

ORF Insert Sequence:

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG GTACACACGC TCTCCTTCTC 
TTACACCCAG TGTCCCGTTT CCTATCTCCT CGCCTCCTAC GGAGAACAAG TCGCCCCCGT 
ATGCGGGCTG TCCCGCTCGC TTCAGGAGGT ATACCTGAGA CTGATGCCCA CCTGCCTTCC 
TTACAGACGG AAGTGTGGAG CTGGGGACAA GGCCAGGATG GACAACTTGG ACATGGAGAC 
CTCCTTCCCA GACTGCAGCC TGATTGTGTA AAGAGTCTAA ATGGTAAGGA GGTGCTGAAA 
GTGGCTGCAG GGGCTCATCA CTCTCTCGCT CTGACAGCAC AGTCACAGGT TTTCTCCTGG 
GGTTGTAACA CTTCTGGACA GCTGGGACAC ATGGAATCGC CCACCACCTT CCCTCAATTG 
ACCAAGCTGT CAGATGGGAT TCGTGTGTGG GATATTGGGG CAGGTCTGCA GCACACTCTC 
TTATTGGCTG ACGGAGACTG TATTCAGCCA ATCCTGTACT ACAGCGGACA GCAGGTGAAG 
GAAGTGCCGG AGCCAACAGA GGAAGAGGAG CAGACTGCAG AATACACGCA ACAACCTGTG 
CTGCTGCCCT TCTGTATGAA CTTGGGCTAT GTAAGCAGTG TGTATGCAGG TGGACGGACG 
TGTGCAGCGC TGACGGATTG TAATGTCATG GGTTTTATTG CTAGTCTGCA TGAGCTGGCG 
GCTGCAGAGA GAAAATATTA CTGCAAACTC AGCAATATCA AGAGTCTTCT GCTCCAGCCA 
TTGCTCAAGC TGGATTCTCT AAGCTCTGCC CTGGGTCAGT CATCTGCAGG GCTGTTGCAG 
AATCTGATTG GTCGAATGAG TCGACTGTCT CAGCTTACAG GTCAAAACTC GGCCTCTCTG 
ACCTCGTTCC TGCAGCGTTC ACGAGACATC CGAGGTCTTG TTATTCTTGA AGATGCACAT 
CTGTTCCTGG ACACATATTC AGAGTACTCT TCTGCAGTTG GTGATCTTTT AGTCATGGGA 
GGATTTCAAG CCCTTGTCAA ACCTTGCCAT GATGTGTTTG GGAAAGGAGC AGAGGTTGTT 
CAGAGGTTGT CTGAATGTTC TGAAGAAGGT TTGACCTTGG CAGATGTTCT GGCGCATCTC 
TTTTACCTGC CCATAAAACA CCTGCATGAA TATGGACGAC TGCTGCTTAA ACTGGCTACC 
TGCTATGAGG TGAGTTCTGT GGACTATCAG AAGCTGCAGG ATGCCTGCTC AAAGTTCGAA 
TCCATGGCTC TTCATCTAAA GAGGAGGAGA AAGGAAGCTG AATACACGCT CCACTTCTGG 
AAGAGTTTTC CTGGAAAGAT GACCGACTCC CTGCGTAAAC CGTCTCGTCG GCTGATCTGT 
GAAAGCAGTA ATAAGGCTTT GACTCTTCAA AACGCAGGAC GTTTTTCCGT CAACTGGTTC 
ATCCTCTTCA ACGACGCACT CGTTCATGCA CAGGGTATGG TCCCCTATAA GAGTCTTTTT 
TCCACCCATC ACATTTTTCC ATTGGCTACG CTGTGGGTGG AGCCAATTTC TGAGGAGAGC 
ACAGGCGTAT ATGGCTTAAA AGTGACTTCG CCTGAGGAAA GCTTCGCTCT GCTGGCTTCA 
TCACCAGCTG AAAAAGCAAA GTGGCTTCGT TCTATAAACC AAGCGGTAGA GCAGGCGCTC 
AGTGGATTGG GGCATGATGG GATACCTCCT TCTACAGGGA TGACGCAGAG AGCAGATCCA 
CCCATCTCCC GGACAGCATC CTACACCTTC TACAAAGACA GTCGGCTAAA GGATGCCAAA 
TATGATGGCC GATGGGTCTC AGGGAAACCC CATGGGCGGG GCGTGGTCAA ATGGCCAGAT 
GGACGAATGT ACACGGGGAC ATTCAAGAAC GGTTTTGAAG ATGGTTTTGG AGATTACGTT 
GTGTCCAATA AAACCCTGAA CAGCTGTGAT CATTATCAAG GCCAGTGGAA AGATGGCAAG 
ATGCACGGTT TTGGAACATT CAGATATGCC TCTGGTGAAG TGTACGAAGG CTCATTTCAA 
GACAACATGC GTCATGGTCA TGGGATGCTG CGTAGTGGGA AGCTGAATTC CACCTCTCCC 
AGTGTCTTTA TCGGCCAGTG GCAGTACGAC AAAAAGTCTG GCTATGGAGT TTTTGATGAC 
ATCACACGAG GAGAGAAGTA TATGGGCATG TGGCTGGATG ATCAACGGCA GGGGAACGGA 
GTTGTTGTTA CACAATTTGG CCTATACTAT GAAGGTGCAT TCAGCAACAA CAAAATGATG 
GGTACTGGGG TGTTACTCTC TGAAGATGAC ACTACATTTG AAGGAGAGTT TTTAGAAGAC 
TGGACTCTTA ATGGAAAGGG TGTGTTGACC ATGCCGAATG GAGACTATAT TGAAGGCTCT 
TTTTGTGGAG TTTGGGGAAC CGGGCTAAAG ATGTCAGGTT CCTACTACAA ACCCAGCCTG 
TACGACTCAG ACAAGGAGAA GGGTCATGCA CTTAAACTGG GCCGGTTAGC AGTTCACTCT 
GAAGAGAAGT GGAAGGCTGT GTTTTTGGAG TGCTGGAACA GGCTCGGTTG TGATTCCCCT 
GTACAGGGGC AAACCACGAC AGCGTGGGAC AACATTGCCA TTGCACTCAC TGCTAACCTG 
AGACAGCAGA GAGACAGTCC AGAGTTGCTG AGCCGCTCAC ATCACAAAAC ACTAGAGAGT 
CTTGAGTGCA TCCCTCAACA TGTGGGTCCT GTAACATTAG AGGTGTACCA CACGATCCGT 
CGGTACCTGG TGAAGGCATG TGATACTCCT CTGCATCCTC TGGGTCGGCT GGTGGAGACG 
CTGGTTTCGG TATACCGGAT GACGTATGTT GGTGTTGGAG CAAACCGCAG GTTATTACCG 
CAGGCCGTCA ATGAGATCAC GTCCTATCTC AGCCGTATCT TCCAGCTCGT CAGATTTCTC 
TTTCCCGAAC TGCCAGAAGA AGGAGGTTCA CTAGCCGACC CTCCAAAAGA GAAGGTCGGC 
ACAGATGCTG ATGGAAAGCT GCTGGAGTCT CCTAAACCTG GGTGTGTGGT CAGCAGCTCT 
GCTCTCCTCC TGCCGGTGCT GTTGCCACGT CTCTATCCTC CTCTCTTCAC GCTCTACGCT 
CTAGAGAAAG AGAAAGAGGA CGATGTGTAC TGGGAGTGTG TGCTGCGACT CAACAAACAG 
CCAGATCTGG CACTGCTGGC TTTCCTCGGG GTCCAACAGA AGTTCTGGCC TGTAACTGTT 
ACAATACATG GAGAAAAACA ACAGGTTCTC TCAAGCACTA AAGACGCCTG TTTCGCCTCA 
GCAGTTGAGA CTTTGCAGCA AATAAGCACA ACGTTCACAC CCTCAGACAA GCTTCAGGTG 
ATCCAGCTGA CCTTCGAGGA AATAACCCAG GAAGTGCTCG CACTCCTGAA ACAGGATTTC 
TTGTGGTCTA TGGATGACCT GTTTCCTGTC TTCCTCTTTG TTGTTCTGCG TGCACGGATA 
AGGAATCTGG GGTCAGAGGT TAGTCTCATT GAGGACCTAA TGGATCCATG TGTACAACAT 
GGAGAGCATG GCATCATGTT CACCACCCTC AAGGCGTGCT ACTATCAAAT CCAGCATGAG 
AAGGTCACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

als2b ( XM_009302176.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300451.1 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X2, mRNA.

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CloneID	ODa49118
Clone ID Related Accession (Same CDS sequence)	XM_009302176.2 , XM_009302176.3
Accession Version	XM_009302176.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	alsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302176.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG GTACACACGC TCTCCTTCTC 
TTACACCCAG TGTCCCGTTT CCTATCTCCT CGCCTCCTAC GGAGAACAAG TCGCCCCCGT 
ATGCGGGCTG TCCCGCTCGC TTCAGGAGGT ATACCTGAGA CTGATGCCCA CCTGCCTTCC 
TTACAGACGG AAGTGTGGAG CTGGGGACAA GGCCAGGATG GACAACTTGG ACATGGAGAC 
CTCCTTCCCA GACTGCAGCC TGATTGTGTA AAGAGTCTAA ATGGTAAGGA GGTGCTGAAA 
GTGGCTGCAG GGGCTCATCA CTCTCTCGCT CTGACAGCAC AGTCACAGGT TTTCTCCTGG 
GGTTGTAACA CTTCTGGACA GCTGGGACAC ATGGAATCGC CCACCACCTT CCCTCAATTG 
ACCAAGCTGT CAGATGGGAT TCGTGTGTGG GATATTGGGG CAGGTCTGCA GCACACTCTC 
TTATTGGCTG ACGGAGACTG TATTCAGCCA ATCCTGTACT ACAGCGGACA GCAGGTGAAG 
GAAGTGCCGG AGCCAACAGA GGAAGAGGAG CAGACTGCAG AATACACGCA ACAACCTGTG 
CTGCTGCCCT TCTGTATGAA CTTGGGCTAT GTAAGCAGTG TGTATGCAGG TGGACGGACG 
TGTGCAGCGC TGACGGATTG TAATGTCATG GGTTTTATTG CTAGTCTGCA TGAGCTGGCG 
GCTGCAGAGA GAAAATATTA CTGCAAACTC AGCAATATCA AGAGTCTTCT GCTCCAGCCA 
TTGCTCAAGC TGGATTCTCT AAGCTCTGCC CTGGGTCAGT CATCTGCAGG GCTGTTGCAG 
AATCTGATTG GTCGAATGAG TCGACTGTCT CAGCTTACAG GTCAAAACTC GGCCTCTCTG 
ACCTCGTTCC TGCAGCGTTC ACGAGACATC CGAGGTCTTG TTATTCTTGA AGATGCACAT 
CTGTTCCTGG ACACATATTC AGAGTACTCT TCTGCAGTTG GTGATCTTTT AGTCATGGGA 
GGATTTCAAG CCCTTGTCAA ACCTTGCCAT GATGTGTTTG GGAAAGGAGC AGAGGTTGTT 
CAGAGGTTGT CTGAATGTTC TGAAGAAGGT TTGACCTTGG CAGATGTTCT GGCGCATCTC 
TTTTACCTGC CCATAAAACA CCTGCATGAA TATGGACGAC TGCTGCTTAA ACTGGCTACC 
TGCTATGAGG TGAGTTCTGT GGACTATCAG AAGCTGCAGG ATGCCTGCTC AAAGTTCGAA 
TCCATGGCTC TTCATCTAAA GAGGAGGAGA AAGGAAGCTG AATACACGCT CCACTTCTGG 
AAGAGTTTTC CTGGAAAGAT GACCGACTCC CTGCGTAAAC CGTCTCGTCG GCTGATCTGT 
GAAAGCAGTA ATAAGGCTTT GACTCTTCAA AACGCAGGAC GTTTTTCCGT CAACTGGTTC 
ATCCTCTTCA ACGACGCACT CGTTCATGCA CAGGGTATGG TCCCCTATAA GAGTCTTTTT 
TCCACCCATC ACATTTTTCC ATTGGCTACG CTGTGGGTGG AGCCAATTTC TGAGGAGAGC 
ACAGGCGTAT ATGGCTTAAA AGTGACTTCG CCTGAGGAAA GCTTCGCTCT GCTGGCTTCA 
TCACCAGCTG AAAAAGCAAA GTGGCTTCGT TCTATAAACC AAGCGGTAGA GCAGGCGCTC 
AGTGGATTGG GGCATGATGG GATACCTCCT TCTACAGGGA TGACGCAGAG AGCAGATCCA 
CCCATCTCCC GGACAGCATC CTACACCTTC TACAAAGACA GTCGGCTAAA GGATGCCAAA 
TATGATGGCC GATGGGTCTC AGGGAAACCC CATGGGCGGG GCGTGGTCAA ATGGCCAGAT 
GGACGAATGT ACACGGGGAC ATTCAAGAAC GGTTTTGAAG ATGGTTTTGG AGATTACGTT 
GTGTCCAATA AAACCCTGAA CAGCTGTGAT CATTATCAAG GCCAGTGGAA AGATGGCAAG 
ATGCACGGTT TTGGAACATT CAGATATGCC TCTGGTGAAG TGTACGAAGG CTCATTTCAA 
GACAACATGC GTCATGGTCA TGGGATGCTG CGTAGTGGGA AGCTGAATTC CACCTCTCCC 
AGTGTCTTTA TCGGCCAGTG GCAGTACGAC AAAAAGTCTG GCTATGGAGT TTTTGATGAC 
ATCACACGAG GAGAGAAGTA TATGGGCATG TGGCTGGATG ATCAACGGCA GGGGAACGGA 
GTTGTTGTTA CACAATTTGG CCTATACTAT GAAGGTGCAT TCAGCAACAA CAAAATGATG 
GGTACTGGGG TGTTACTCTC TGAAGATGAC ACTACATTTG AAGGAGAGTT TTTAGAAGAC 
TGGACTCTTA ATGGAAAGGG TGTGTTGACC ATGCCGAATG GAGACTATAT TGAAGGCTCT 
TTTTGTGGAG TTTGGGGAAC CGGGCTAAAG ATGTCAGGTT CCTACTACAA ACCCAGCCTG 
TACGACTCAG ACAAGGAGAA GGGTCATGCA CTTAAACTGG GCCGGTTAGC AGTTCACTCT 
GAAGAGAAGT GGAAGGCTGT GTTTTTGGAG TGCTGGAACA GGCTCGGTTG TGATTCCCCT 
GTACAGGGGC AAACCACGAC AGCGTGGGAC AACATTGCCA TTGCACTCAC TGCTAACCTG 
AGACAGCAGA GAGACAGTCC AGAGTTGCTG AGCCGCTCAC ATCACAAAAC ACTAGAGAGT 
CTTGAGTGCA TCCCTCAACA TGTGGGTCCT GTAACATTAG AGGTGTACCA CACGATCCGT 
CGGTACCTGG TGAAGGCATG TGATACTCCT CTGCATCCTC TGGGTCGGCT GGTGGAGACG 
CTGGTTTCGG TATACCGGAT GACGTATGTT GGTGTTGGAG CAAACCGCAG GTTATTACCG 
CAGGCCGTCA ATGAGATCAC GTCCTATCTC AGCCGTATCT TCCAGCTCGT CAGATTTCTC 
TTTCCCGAAC TGCCAGAAGA AGGAGGTTCA CTAGCCGACC CTCCAAAAGA GAAGGTCGGC 
ACAGATGCTG ATGGAAAGCT GCTGGAGTCT CCTAAACCTG GGTGTGTGGT CAGCAGCTCT 
GCTCTCCTCC TGCCGGTGCT GTTGCCACGT CTCTATCCTC CTCTCTTCAC GCTCTACGCT 
CTAGAGAAAG AGAAAGAGGA CGATGTGTAC TGGGAGTGTG TGCTGCGACT CAACAAACAG 
CCAGATCTGG CACTGCTGGC TTTCCTCGGG GTCCAACAGA AGTTCTGGCC TGTAACTGTT 
ACAATACATG GAGAAAAACA ACAGGTTCTC TCAAGCACTA AAGACGCCTG TTTCGCCTCA 
GCAGTTGAGA CTTTGCAGCA AATAAGCACA ACGTTCACAC CCTCAGACAA GCTTCAGGTG 
ATCCAGCTGA CCTTCGAGGA AATAACCCAG GAAGTGCTCG CACTCCTGAA ACAGGATTTC 
TTGTGGTCTA TGGATGACCT GTTTCCTGTC TTCCTCTTTG TTGTTCTGCG TGCACGGATA 
AGGAATCTGG GGTCAGAGGT TAGTCTCATT GAGGACCTAA TGGATCCATG TGTACAACAT 
GGAGAGCATG GCATCATGTT CACCACCCTC AAGGCGTGCT ACTATCAAAT CCAGCATGAG 
AAGGTCACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300451.1
CDS	270..5381
Translation	MEKLISSGEGDSSGERGLLHTWKGYSYSTAPERVLLSRPVLQAALGARHGVLLVEGGQVYSFGELPWKQNQTSSAEPILEAVLSEQRVVFVAAGSAHSGVVTEDGGVHMWGENVHGQCGLLGLSVIPNPTPVGVLDSEATPPQTVKILEVACGDQHTLALSAKHEVWAWGSGCQLGLNTSTFPVWKPQKVDHLSGRHVLQVACGSAHSVALVRCLPPPQEPRKPPQDKCGQCHQLLYTMTDKEDHVIISDGHHCPLGVEMDSTEIKSAVEGSPDPGKPGKSSPTEPVPCMSTQISKSPTSSNISESTRPPESTLPFELQNQVPAYCQESIEAPVEDSAAHEAPAAAGEVCVEASENSIPEPDSCRSSGKSPYPDEQAVKAYLKRLSDHALAEHTIKTSSTIHSAQLSGDPANLFASEPLIPVAQSVTPMGSALNNLVVSCASAVGERVVSTYEALSLRKVIGYWVPGEGRERVEERLRLEESMQGKKSSSLGDIREEEAELSRRLSLPGLLSQGTHALLLLHPVSRFLSPRLLRRTSRPRMRAVPLASGGIPETDAHLPSLQTEVWSWGQGQDGQLGHGDLLPRLQPDCVKSLNGKEVLKVAAGAHHSLALTAQSQVFSWGCNTSGQLGHMESPTTFPQLTKLSDGIRVWDIGAGLQHTLLLADGDCIQPILYYSGQQVKEVPEPTEEEEQTAEYTQQPVLLPFCMNLGYVSSVYAGGRTCAALTDCNVMGFIASLHELAAAERKYYCKLSNIKSLLLQPLLKLDSLSSALGQSSAGLLQNLIGRMSRLSQLTGQNSASLTSFLQRSRDIRGLVILEDAHLFLDTYSEYSSAVGDLLVMGGFQALVKPCHDVFGKGAEVVQRLSECSEEGLTLADVLAHLFYLPIKHLHEYGRLLLKLATCYEVSSVDYQKLQDACSKFESMALHLKRRRKEAEYTLHFWKSFPGKMTDSLRKPSRRLICESSNKALTLQNAGRFSVNWFILFNDALVHAQGMVPYKSLFSTHHIFPLATLWVEPISEESTGVYGLKVTSPEESFALLASSPAEKAKWLRSINQAVEQALSGLGHDGIPPSTGMTQRADPPISRTASYTFYKDSRLKDAKYDGRWVSGKPHGRGVVKWPDGRMYTGTFKNGFEDGFGDYVVSNKTLNSCDHYQGQWKDGKMHGFGTFRYASGEVYEGSFQDNMRHGHGMLRSGKLNSTSPSVFIGQWQYDKKSGYGVFDDITRGEKYMGMWLDDQRQGNGVVVTQFGLYYEGAFSNNKMMGTGVLLSEDDTTFEGEFLEDWTLNGKGVLTMPNGDYIEGSFCGVWGTGLKMSGSYYKPSLYDSDKEKGHALKLGRLAVHSEEKWKAVFLECWNRLGCDSPVQGQTTTAWDNIAIALTANLRQQRDSPELLSRSHHKTLESLECIPQHVGPVTLEVYHTIRRYLVKACDTPLHPLGRLVETLVSVYRMTYVGVGANRRLLPQAVNEITSYLSRIFQLVRFLFPELPEEGGSLADPPKEKVGTDADGKLLESPKPGCVVSSSALLLPVLLPRLYPPLFTLYALEKEKEDDVYWECVLRLNKQPDLALLAFLGVQQKFWPVTVTIHGEKQQVLSSTKDACFASAVETLQQISTTFTPSDKLQVIQLTFEEITQEVLALLKQDFLWSMDDLFPVFLFVVLRARIRNLGSEVSLIEDLMDPCVQHGEHGIMFTTLKACYYQIQHEKVT

Target ORF information:

RefSeq Version	XM_009302176.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302176.3

ORF Insert Sequence:

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG GTACACACGC TCTCCTTCTC 
TTACACCCAG TGTCCCGTTT CCTATCTCCT CGCCTCCTAC GGAGAACAAG TCGCCCCCGT 
ATGCGGGCTG TCCCGCTCGC TTCAGGAGGT ATACCTGAGA CTGATGCCCA CCTGCCTTCC 
TTACAGACGG AAGTGTGGAG CTGGGGACAA GGCCAGGATG GACAACTTGG ACATGGAGAC 
CTCCTTCCCA GACTGCAGCC TGATTGTGTA AAGAGTCTAA ATGGTAAGGA GGTGCTGAAA 
GTGGCTGCAG GGGCTCATCA CTCTCTCGCT CTGACAGCAC AGTCACAGGT TTTCTCCTGG 
GGTTGTAACA CTTCTGGACA GCTGGGACAC ATGGAATCGC CCACCACCTT CCCTCAATTG 
ACCAAGCTGT CAGATGGGAT TCGTGTGTGG GATATTGGGG CAGGTCTGCA GCACACTCTC 
TTATTGGCTG ACGGAGACTG TATTCAGCCA ATCCTGTACT ACAGCGGACA GCAGGTGAAG 
GAAGTGCCGG AGCCAACAGA GGAAGAGGAG CAGACTGCAG AATACACGCA ACAACCTGTG 
CTGCTGCCCT TCTGTATGAA CTTGGGCTAT GTAAGCAGTG TGTATGCAGG TGGACGGACG 
TGTGCAGCGC TGACGGATTG TAATGTCATG GGTTTTATTG CTAGTCTGCA TGAGCTGGCG 
GCTGCAGAGA GAAAATATTA CTGCAAACTC AGCAATATCA AGAGTCTTCT GCTCCAGCCA 
TTGCTCAAGC TGGATTCTCT AAGCTCTGCC CTGGGTCAGT CATCTGCAGG GCTGTTGCAG 
AATCTGATTG GTCGAATGAG TCGACTGTCT CAGCTTACAG GTCAAAACTC GGCCTCTCTG 
ACCTCGTTCC TGCAGCGTTC ACGAGACATC CGAGGTCTTG TTATTCTTGA AGATGCACAT 
CTGTTCCTGG ACACATATTC AGAGTACTCT TCTGCAGTTG GTGATCTTTT AGTCATGGGA 
GGATTTCAAG CCCTTGTCAA ACCTTGCCAT GATGTGTTTG GGAAAGGAGC AGAGGTTGTT 
CAGAGGTTGT CTGAATGTTC TGAAGAAGGT TTGACCTTGG CAGATGTTCT GGCGCATCTC 
TTTTACCTGC CCATAAAACA CCTGCATGAA TATGGACGAC TGCTGCTTAA ACTGGCTACC 
TGCTATGAGG TGAGTTCTGT GGACTATCAG AAGCTGCAGG ATGCCTGCTC AAAGTTCGAA 
TCCATGGCTC TTCATCTAAA GAGGAGGAGA AAGGAAGCTG AATACACGCT CCACTTCTGG 
AAGAGTTTTC CTGGAAAGAT GACCGACTCC CTGCGTAAAC CGTCTCGTCG GCTGATCTGT 
GAAAGCAGTA ATAAGGCTTT GACTCTTCAA AACGCAGGAC GTTTTTCCGT CAACTGGTTC 
ATCCTCTTCA ACGACGCACT CGTTCATGCA CAGGGTATGG TCCCCTATAA GAGTCTTTTT 
TCCACCCATC ACATTTTTCC ATTGGCTACG CTGTGGGTGG AGCCAATTTC TGAGGAGAGC 
ACAGGCGTAT ATGGCTTAAA AGTGACTTCG CCTGAGGAAA GCTTCGCTCT GCTGGCTTCA 
TCACCAGCTG AAAAAGCAAA GTGGCTTCGT TCTATAAACC AAGCGGTAGA GCAGGCGCTC 
AGTGGATTGG GGCATGATGG GATACCTCCT TCTACAGGGA TGACGCAGAG AGCAGATCCA 
CCCATCTCCC GGACAGCATC CTACACCTTC TACAAAGACA GTCGGCTAAA GGATGCCAAA 
TATGATGGCC GATGGGTCTC AGGGAAACCC CATGGGCGGG GCGTGGTCAA ATGGCCAGAT 
GGACGAATGT ACACGGGGAC ATTCAAGAAC GGTTTTGAAG ATGGTTTTGG AGATTACGTT 
GTGTCCAATA AAACCCTGAA CAGCTGTGAT CATTATCAAG GCCAGTGGAA AGATGGCAAG 
ATGCACGGTT TTGGAACATT CAGATATGCC TCTGGTGAAG TGTACGAAGG CTCATTTCAA 
GACAACATGC GTCATGGTCA TGGGATGCTG CGTAGTGGGA AGCTGAATTC CACCTCTCCC 
AGTGTCTTTA TCGGCCAGTG GCAGTACGAC AAAAAGTCTG GCTATGGAGT TTTTGATGAC 
ATCACACGAG GAGAGAAGTA TATGGGCATG TGGCTGGATG ATCAACGGCA GGGGAACGGA 
GTTGTTGTTA CACAATTTGG CCTATACTAT GAAGGTGCAT TCAGCAACAA CAAAATGATG 
GGTACTGGGG TGTTACTCTC TGAAGATGAC ACTACATTTG AAGGAGAGTT TTTAGAAGAC 
TGGACTCTTA ATGGAAAGGG TGTGTTGACC ATGCCGAATG GAGACTATAT TGAAGGCTCT 
TTTTGTGGAG TTTGGGGAAC CGGGCTAAAG ATGTCAGGTT CCTACTACAA ACCCAGCCTG 
TACGACTCAG ACAAGGAGAA GGGTCATGCA CTTAAACTGG GCCGGTTAGC AGTTCACTCT 
GAAGAGAAGT GGAAGGCTGT GTTTTTGGAG TGCTGGAACA GGCTCGGTTG TGATTCCCCT 
GTACAGGGGC AAACCACGAC AGCGTGGGAC AACATTGCCA TTGCACTCAC TGCTAACCTG 
AGACAGCAGA GAGACAGTCC AGAGTTGCTG AGCCGCTCAC ATCACAAAAC ACTAGAGAGT 
CTTGAGTGCA TCCCTCAACA TGTGGGTCCT GTAACATTAG AGGTGTACCA CACGATCCGT 
CGGTACCTGG TGAAGGCATG TGATACTCCT CTGCATCCTC TGGGTCGGCT GGTGGAGACG 
CTGGTTTCGG TATACCGGAT GACGTATGTT GGTGTTGGAG CAAACCGCAG GTTATTACCG 
CAGGCCGTCA ATGAGATCAC GTCCTATCTC AGCCGTATCT TCCAGCTCGT CAGATTTCTC 
TTTCCCGAAC TGCCAGAAGA AGGAGGTTCA CTAGCCGACC CTCCAAAAGA GAAGGTCGGC 
ACAGATGCTG ATGGAAAGCT GCTGGAGTCT CCTAAACCTG GGTGTGTGGT CAGCAGCTCT 
GCTCTCCTCC TGCCGGTGCT GTTGCCACGT CTCTATCCTC CTCTCTTCAC GCTCTACGCT 
CTAGAGAAAG AGAAAGAGGA CGATGTGTAC TGGGAGTGTG TGCTGCGACT CAACAAACAG 
CCAGATCTGG CACTGCTGGC TTTCCTCGGG GTCCAACAGA AGTTCTGGCC TGTAACTGTT 
ACAATACATG GAGAAAAACA ACAGGTTCTC TCAAGCACTA AAGACGCCTG TTTCGCCTCA 
GCAGTTGAGA CTTTGCAGCA AATAAGCACA ACGTTCACAC CCTCAGACAA GCTTCAGGTG 
ATCCAGCTGA CCTTCGAGGA AATAACCCAG GAAGTGCTCG CACTCCTGAA ACAGGATTTC 
TTGTGGTCTA TGGATGACCT GTTTCCTGTC TTCCTCTTTG TTGTTCTGCG TGCACGGATA 
AGGAATCTGG GGTCAGAGGT TAGTCTCATT GAGGACCTAA TGGATCCATG TGTACAACAT 
GGAGAGCATG GCATCATGTT CACCACCCTC AAGGCGTGCT ACTATCAAAT CCAGCATGAG 
AAGGTCACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

als2b ( XM_009302177.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300452.1 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X4, mRNA.

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CloneID	ODa49119
Clone ID Related Accession (Same CDS sequence)	XM_009302177.2 , XM_009302177.3
Accession Version	XM_009302177.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	alsin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG 
AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT 
GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA 
CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT 
GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG 
TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC 
ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA 
GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC 
AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA 
TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG 
TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT 
AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG 
AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA 
TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT 
CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT 
ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT 
GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG 
AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA 
GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG 
CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT 
GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA 
TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG 
TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT 
TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GGGTATGGTC 
CCCTATAAGA GTCTTTTTTC CACCCATCAC ATTTTTCCAT TGGCTACGCT GTGGGTGGAG 
CCAATTTCTG AGGAGAGCAC AGGCGTATAT GGCTTAAAAG TGACTTCGCC TGAGGAAAGC 
TTCGCTCTGC TGGCTTCATC ACCAGCTGAA AAAGCAAAGT GGCTTCGTTC TATAAACCAA 
GCGGTAGAGC AGGCGCTCAG TGGATTGGGG CATGATGGGA TACCTCCTTC TACAGGGATG 
ACGCAGAGAG CAGATCCACC CATCTCCCGG ACAGCATCCT ACACCTTCTA CAAAGACAGT 
CGGCTAAAGG ATGCCAAATA TGATGGCCGA TGGGTCTCAG GGAAACCCCA TGGGCGGGGC 
GTGGTCAAAT GGCCAGATGG ACGAATGTAC ACGGGGACAT TCAAGAACGG TTTTGAAGAT 
GGTTTTGGAG ATTACGTTGT GTCCAATAAA ACCCTGAACA GCTGTGATCA TTATCAAGGC 
CAGTGGAAAG ATGGCAAGAT GCACGGTTTT GGAACATTCA GATATGCCTC TGGTGAAGTG 
TACGAAGGCT CATTTCAAGA CAACATGCGT CATGGTCATG GGATGCTGCG TAGTGGGAAG 
CTGAATTCCA CCTCTCCCAG TGTCTTTATC GGCCAGTGGC AGTACGACAA AAAGTCTGGC 
TATGGAGTTT TTGATGACAT CACACGAGGA GAGAAGTATA TGGGCATGTG GCTGGATGAT 
CAACGGCAGG GGAACGGAGT TGTTGTTACA CAATTTGGCC TATACTATGA AGGTGCATTC 
AGCAACAACA AAATGATGGG TACTGGGGTG TTACTCTCTG AAGATGACAC TACATTTGAA 
GGAGAGTTTT TAGAAGACTG GACTCTTAAT GGAAAGGGTG TGTTGACCAT GCCGAATGGA 
GACTATATTG AAGGCTCTTT TTGTGGAGTT TGGGGAACCG GGCTAAAGAT GTCAGGTTCC 
TACTACAAAC CCAGCCTGTA CGACTCAGAC AAGGAGAAGG GTCATGCACT TAAACTGGGC 
CGGTTAGCAG TTCACTCTGA AGAGAAGTGG AAGGCTGTGT TTTTGGAGTG CTGGAACAGG 
CTCGGTTGTG ATTCCCCTGT ACAGGGGCAA ACCACGACAG CGTGGGACAA CATTGCCATT 
GCACTCACTG CTAACCTGAG ACAGCAGAGA GACAGTCCAG AGTTGCTGAG CCGCTCACAT 
CACAAAACAC TAGAGAGTCT TGAGTGCATC CCTCAACATG TGGGTCCTGT AACATTAGAG 
GTGTACCACA CGATCCGTCG GTACCTGGTG AAGGCATGTG ATACTCCTCT GCATCCTCTG 
GGTCGGCTGG TGGAGACGCT GGTTTCGGTA TACCGGATGA CGTATGTTGG TGTTGGAGCA 
AACCGCAGGT TATTACCGCA GGCCGTCAAT GAGATCACGT CCTATCTCAG CCGTATCTTC 
CAGCTCGTCA GATTTCTCTT TCCCGAACTG CCAGAAGAAG GAGGTTCACT AGCCGACCCT 
CCAAAAGAGA AGGTCGGCAC AGATGCTGAT GGAAAGCTGC TGGAGTCTCC TAAACCTGGG 
TGTGTGGTCA GCAGCTCTGC TCTCCTCCTG CCGGTGCTGT TGCCACGTCT CTATCCTCCT 
CTCTTCACGC TCTACGCTCT AGAGAAAGAG AAAGAGGACG ATGTGTACTG GGAGTGTGTG 
CTGCGACTCA ACAAACAGCC AGATCTGGCA CTGCTGGCTT TCCTCGGGGT CCAACAGAAG 
TTCTGGCCTG TAACTGTTAC AATACATGGA GAAAAACAAC AGGTTCTCTC AAGCACTAAA 
GACGCCTGTT TCGCCTCAGC AGTTGAGACT TTGCAGCAAA TAAGCACAAC GTTCACACCC 
TCAGACAAGC TTCAGGTGAT CCAGCTGACC TTCGAGGAAA TAACCCAGGA AGTGCTCGCA 
CTCCTGAAAC AGGATTTCTT GTGGTCTATG GATGACCTGT TTCCTGTCTT CCTCTTTGTT 
GTTCTGCGTG CACGGATAAG GAATCTGGGG TCAGAGGTTA GTCTCATTGA GGACCTAATG 
GATCCATGTG TACAACATGG AGAGCATGGC ATCATGTTCA CCACCCTCAA GGCGTGCTAC 
TATCAAATCC AGCATGAGAA GGTCACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300452.1
CDS	250..5319
Translation	MEKLISSGEGDSSGERGLLHTWKGYSYSTAPERVLLSRPVLQAALGARHGVLLVEGGQVYSFGELPWKQNQTSSAEPILEAVLSEQRVVFVAAGSAHSGVVTEDGGVHMWGENVHGQCGLLGLSVIPNPTPVGVLDSEATPPQTVKILEVACGDQHTLALSAKHEVWAWGSGCQLGLNTSTFPVWKPQKVDHLSGRHVLQVACGSAHSVALVRCLPPPQEPRKPPQDKCGQCHQLLYTMTDKEDHVIISDGHHCPLGVEMDSTEIKSAVEGSPDPGKPGKSSPTEPVPCMSTQISKSPTSSNISESTRPPESTLPFELQNQVPAYCQESIEAPVEDSAAHEAPAAAGEVCVEASENSIPEPDSCRSSGKSPYPDEQAVKAYLKRLSDHALAEHTIKTSSTIHSAQLSGDPANLFASEPLIPVAQSVTPMGSALNNLVVSCASAVGERVVSTYEALSLRKVIGYWVPGEGRERVEERLRLEESMQGKKSSSLGDIREEEAELSRRLSLPGLLSQVSPRLLRRTSRPRMRAVPLASGGIPETDAHLPSLQTEVWSWGQGQDGQLGHGDLLPRLQPDCVKSLNGKEVLKVAAGAHHSLALTAQSQVFSWGCNTSGQLGHMESPTTFPQLTKLSDGIRVWDIGAGLQHTLLLADGDCIQPILYYSGQQVKEVPEPTEEEEQTAEYTQQPVLLPFCMNLGYVSSVYAGGRTCAALTDCNVMGFIASLHELAAAERKYYCKLSNIKSLLLQPLLKLDSLSSALGQSSAGLLQNLIGRMSRLSQLTGQNSASLTSFLQRSRDIRGLVILEDAHLFLDTYSEYSSAVGDLLVMGGFQALVKPCHDVFGKGAEVVQRLSECSEEGLTLADVLAHLFYLPIKHLHEYGRLLLKLATCYEVSSVDYQKLQDACSKFESMALHLKRRRKEAEYTLHFWKSFPGKMTDSLRKPSRRLICESSNKALTLQNAGRFSVNWFILFNDALVHAQGMVPYKSLFSTHHIFPLATLWVEPISEESTGVYGLKVTSPEESFALLASSPAEKAKWLRSINQAVEQALSGLGHDGIPPSTGMTQRADPPISRTASYTFYKDSRLKDAKYDGRWVSGKPHGRGVVKWPDGRMYTGTFKNGFEDGFGDYVVSNKTLNSCDHYQGQWKDGKMHGFGTFRYASGEVYEGSFQDNMRHGHGMLRSGKLNSTSPSVFIGQWQYDKKSGYGVFDDITRGEKYMGMWLDDQRQGNGVVVTQFGLYYEGAFSNNKMMGTGVLLSEDDTTFEGEFLEDWTLNGKGVLTMPNGDYIEGSFCGVWGTGLKMSGSYYKPSLYDSDKEKGHALKLGRLAVHSEEKWKAVFLECWNRLGCDSPVQGQTTTAWDNIAIALTANLRQQRDSPELLSRSHHKTLESLECIPQHVGPVTLEVYHTIRRYLVKACDTPLHPLGRLVETLVSVYRMTYVGVGANRRLLPQAVNEITSYLSRIFQLVRFLFPELPEEGGSLADPPKEKVGTDADGKLLESPKPGCVVSSSALLLPVLLPRLYPPLFTLYALEKEKEDDVYWECVLRLNKQPDLALLAFLGVQQKFWPVTVTIHGEKQQVLSSTKDACFASAVETLQQISTTFTPSDKLQVIQLTFEEITQEVLALLKQDFLWSMDDLFPVFLFVVLRARIRNLGSEVSLIEDLMDPCVQHGEHGIMFTTLKACYYQIQHEKVT

Target ORF information:

RefSeq Version	XM_009302177.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302177.2

ORF Insert Sequence:

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG 
AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT 
GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA 
CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT 
GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG 
TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC 
ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA 
GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC 
AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA 
TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG 
TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT 
AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG 
AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA 
TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT 
CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT 
ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT 
GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG 
AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA 
GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG 
CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT 
GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA 
TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG 
TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT 
TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GGGTATGGTC 
CCCTATAAGA GTCTTTTTTC CACCCATCAC ATTTTTCCAT TGGCTACGCT GTGGGTGGAG 
CCAATTTCTG AGGAGAGCAC AGGCGTATAT GGCTTAAAAG TGACTTCGCC TGAGGAAAGC 
TTCGCTCTGC TGGCTTCATC ACCAGCTGAA AAAGCAAAGT GGCTTCGTTC TATAAACCAA 
GCGGTAGAGC AGGCGCTCAG TGGATTGGGG CATGATGGGA TACCTCCTTC TACAGGGATG 
ACGCAGAGAG CAGATCCACC CATCTCCCGG ACAGCATCCT ACACCTTCTA CAAAGACAGT 
CGGCTAAAGG ATGCCAAATA TGATGGCCGA TGGGTCTCAG GGAAACCCCA TGGGCGGGGC 
GTGGTCAAAT GGCCAGATGG ACGAATGTAC ACGGGGACAT TCAAGAACGG TTTTGAAGAT 
GGTTTTGGAG ATTACGTTGT GTCCAATAAA ACCCTGAACA GCTGTGATCA TTATCAAGGC 
CAGTGGAAAG ATGGCAAGAT GCACGGTTTT GGAACATTCA GATATGCCTC TGGTGAAGTG 
TACGAAGGCT CATTTCAAGA CAACATGCGT CATGGTCATG GGATGCTGCG TAGTGGGAAG 
CTGAATTCCA CCTCTCCCAG TGTCTTTATC GGCCAGTGGC AGTACGACAA AAAGTCTGGC 
TATGGAGTTT TTGATGACAT CACACGAGGA GAGAAGTATA TGGGCATGTG GCTGGATGAT 
CAACGGCAGG GGAACGGAGT TGTTGTTACA CAATTTGGCC TATACTATGA AGGTGCATTC 
AGCAACAACA AAATGATGGG TACTGGGGTG TTACTCTCTG AAGATGACAC TACATTTGAA 
GGAGAGTTTT TAGAAGACTG GACTCTTAAT GGAAAGGGTG TGTTGACCAT GCCGAATGGA 
GACTATATTG AAGGCTCTTT TTGTGGAGTT TGGGGAACCG GGCTAAAGAT GTCAGGTTCC 
TACTACAAAC CCAGCCTGTA CGACTCAGAC AAGGAGAAGG GTCATGCACT TAAACTGGGC 
CGGTTAGCAG TTCACTCTGA AGAGAAGTGG AAGGCTGTGT TTTTGGAGTG CTGGAACAGG 
CTCGGTTGTG ATTCCCCTGT ACAGGGGCAA ACCACGACAG CGTGGGACAA CATTGCCATT 
GCACTCACTG CTAACCTGAG ACAGCAGAGA GACAGTCCAG AGTTGCTGAG CCGCTCACAT 
CACAAAACAC TAGAGAGTCT TGAGTGCATC CCTCAACATG TGGGTCCTGT AACATTAGAG 
GTGTACCACA CGATCCGTCG GTACCTGGTG AAGGCATGTG ATACTCCTCT GCATCCTCTG 
GGTCGGCTGG TGGAGACGCT GGTTTCGGTA TACCGGATGA CGTATGTTGG TGTTGGAGCA 
AACCGCAGGT TATTACCGCA GGCCGTCAAT GAGATCACGT CCTATCTCAG CCGTATCTTC 
CAGCTCGTCA GATTTCTCTT TCCCGAACTG CCAGAAGAAG GAGGTTCACT AGCCGACCCT 
CCAAAAGAGA AGGTCGGCAC AGATGCTGAT GGAAAGCTGC TGGAGTCTCC TAAACCTGGG 
TGTGTGGTCA GCAGCTCTGC TCTCCTCCTG CCGGTGCTGT TGCCACGTCT CTATCCTCCT 
CTCTTCACGC TCTACGCTCT AGAGAAAGAG AAAGAGGACG ATGTGTACTG GGAGTGTGTG 
CTGCGACTCA ACAAACAGCC AGATCTGGCA CTGCTGGCTT TCCTCGGGGT CCAACAGAAG 
TTCTGGCCTG TAACTGTTAC AATACATGGA GAAAAACAAC AGGTTCTCTC AAGCACTAAA 
GACGCCTGTT TCGCCTCAGC AGTTGAGACT TTGCAGCAAA TAAGCACAAC GTTCACACCC 
TCAGACAAGC TTCAGGTGAT CCAGCTGACC TTCGAGGAAA TAACCCAGGA AGTGCTCGCA 
CTCCTGAAAC AGGATTTCTT GTGGTCTATG GATGACCTGT TTCCTGTCTT CCTCTTTGTT 
GTTCTGCGTG CACGGATAAG GAATCTGGGG TCAGAGGTTA GTCTCATTGA GGACCTAATG 
GATCCATGTG TACAACATGG AGAGCATGGC ATCATGTTCA CCACCCTCAA GGCGTGCTAC 
TATCAAATCC AGCATGAGAA GGTCACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

als2b ( XM_009302177.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300452.1 Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa49119
Clone ID Related Accession (Same CDS sequence)	XM_009302177.2 , XM_009302177.3
Accession Version	XM_009302177.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	alsin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG 
AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT 
GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA 
CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT 
GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG 
TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC 
ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA 
GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC 
AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA 
TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG 
TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT 
AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG 
AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA 
TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT 
CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT 
ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT 
GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG 
AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA 
GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG 
CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT 
GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA 
TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG 
TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT 
TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GGGTATGGTC 
CCCTATAAGA GTCTTTTTTC CACCCATCAC ATTTTTCCAT TGGCTACGCT GTGGGTGGAG 
CCAATTTCTG AGGAGAGCAC AGGCGTATAT GGCTTAAAAG TGACTTCGCC TGAGGAAAGC 
TTCGCTCTGC TGGCTTCATC ACCAGCTGAA AAAGCAAAGT GGCTTCGTTC TATAAACCAA 
GCGGTAGAGC AGGCGCTCAG TGGATTGGGG CATGATGGGA TACCTCCTTC TACAGGGATG 
ACGCAGAGAG CAGATCCACC CATCTCCCGG ACAGCATCCT ACACCTTCTA CAAAGACAGT 
CGGCTAAAGG ATGCCAAATA TGATGGCCGA TGGGTCTCAG GGAAACCCCA TGGGCGGGGC 
GTGGTCAAAT GGCCAGATGG ACGAATGTAC ACGGGGACAT TCAAGAACGG TTTTGAAGAT 
GGTTTTGGAG ATTACGTTGT GTCCAATAAA ACCCTGAACA GCTGTGATCA TTATCAAGGC 
CAGTGGAAAG ATGGCAAGAT GCACGGTTTT GGAACATTCA GATATGCCTC TGGTGAAGTG 
TACGAAGGCT CATTTCAAGA CAACATGCGT CATGGTCATG GGATGCTGCG TAGTGGGAAG 
CTGAATTCCA CCTCTCCCAG TGTCTTTATC GGCCAGTGGC AGTACGACAA AAAGTCTGGC 
TATGGAGTTT TTGATGACAT CACACGAGGA GAGAAGTATA TGGGCATGTG GCTGGATGAT 
CAACGGCAGG GGAACGGAGT TGTTGTTACA CAATTTGGCC TATACTATGA AGGTGCATTC 
AGCAACAACA AAATGATGGG TACTGGGGTG TTACTCTCTG AAGATGACAC TACATTTGAA 
GGAGAGTTTT TAGAAGACTG GACTCTTAAT GGAAAGGGTG TGTTGACCAT GCCGAATGGA 
GACTATATTG AAGGCTCTTT TTGTGGAGTT TGGGGAACCG GGCTAAAGAT GTCAGGTTCC 
TACTACAAAC CCAGCCTGTA CGACTCAGAC AAGGAGAAGG GTCATGCACT TAAACTGGGC 
CGGTTAGCAG TTCACTCTGA AGAGAAGTGG AAGGCTGTGT TTTTGGAGTG CTGGAACAGG 
CTCGGTTGTG ATTCCCCTGT ACAGGGGCAA ACCACGACAG CGTGGGACAA CATTGCCATT 
GCACTCACTG CTAACCTGAG ACAGCAGAGA GACAGTCCAG AGTTGCTGAG CCGCTCACAT 
CACAAAACAC TAGAGAGTCT TGAGTGCATC CCTCAACATG TGGGTCCTGT AACATTAGAG 
GTGTACCACA CGATCCGTCG GTACCTGGTG AAGGCATGTG ATACTCCTCT GCATCCTCTG 
GGTCGGCTGG TGGAGACGCT GGTTTCGGTA TACCGGATGA CGTATGTTGG TGTTGGAGCA 
AACCGCAGGT TATTACCGCA GGCCGTCAAT GAGATCACGT CCTATCTCAG CCGTATCTTC 
CAGCTCGTCA GATTTCTCTT TCCCGAACTG CCAGAAGAAG GAGGTTCACT AGCCGACCCT 
CCAAAAGAGA AGGTCGGCAC AGATGCTGAT GGAAAGCTGC TGGAGTCTCC TAAACCTGGG 
TGTGTGGTCA GCAGCTCTGC TCTCCTCCTG CCGGTGCTGT TGCCACGTCT CTATCCTCCT 
CTCTTCACGC TCTACGCTCT AGAGAAAGAG AAAGAGGACG ATGTGTACTG GGAGTGTGTG 
CTGCGACTCA ACAAACAGCC AGATCTGGCA CTGCTGGCTT TCCTCGGGGT CCAACAGAAG 
TTCTGGCCTG TAACTGTTAC AATACATGGA GAAAAACAAC AGGTTCTCTC AAGCACTAAA 
GACGCCTGTT TCGCCTCAGC AGTTGAGACT TTGCAGCAAA TAAGCACAAC GTTCACACCC 
TCAGACAAGC TTCAGGTGAT CCAGCTGACC TTCGAGGAAA TAACCCAGGA AGTGCTCGCA 
CTCCTGAAAC AGGATTTCTT GTGGTCTATG GATGACCTGT TTCCTGTCTT CCTCTTTGTT 
GTTCTGCGTG CACGGATAAG GAATCTGGGG TCAGAGGTTA GTCTCATTGA GGACCTAATG 
GATCCATGTG TACAACATGG AGAGCATGGC ATCATGTTCA CCACCCTCAA GGCGTGCTAC 
TATCAAATCC AGCATGAGAA GGTCACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300452.1
CDS	270..5339
Translation	MEKLISSGEGDSSGERGLLHTWKGYSYSTAPERVLLSRPVLQAALGARHGVLLVEGGQVYSFGELPWKQNQTSSAEPILEAVLSEQRVVFVAAGSAHSGVVTEDGGVHMWGENVHGQCGLLGLSVIPNPTPVGVLDSEATPPQTVKILEVACGDQHTLALSAKHEVWAWGSGCQLGLNTSTFPVWKPQKVDHLSGRHVLQVACGSAHSVALVRCLPPPQEPRKPPQDKCGQCHQLLYTMTDKEDHVIISDGHHCPLGVEMDSTEIKSAVEGSPDPGKPGKSSPTEPVPCMSTQISKSPTSSNISESTRPPESTLPFELQNQVPAYCQESIEAPVEDSAAHEAPAAAGEVCVEASENSIPEPDSCRSSGKSPYPDEQAVKAYLKRLSDHALAEHTIKTSSTIHSAQLSGDPANLFASEPLIPVAQSVTPMGSALNNLVVSCASAVGERVVSTYEALSLRKVIGYWVPGEGRERVEERLRLEESMQGKKSSSLGDIREEEAELSRRLSLPGLLSQVSPRLLRRTSRPRMRAVPLASGGIPETDAHLPSLQTEVWSWGQGQDGQLGHGDLLPRLQPDCVKSLNGKEVLKVAAGAHHSLALTAQSQVFSWGCNTSGQLGHMESPTTFPQLTKLSDGIRVWDIGAGLQHTLLLADGDCIQPILYYSGQQVKEVPEPTEEEEQTAEYTQQPVLLPFCMNLGYVSSVYAGGRTCAALTDCNVMGFIASLHELAAAERKYYCKLSNIKSLLLQPLLKLDSLSSALGQSSAGLLQNLIGRMSRLSQLTGQNSASLTSFLQRSRDIRGLVILEDAHLFLDTYSEYSSAVGDLLVMGGFQALVKPCHDVFGKGAEVVQRLSECSEEGLTLADVLAHLFYLPIKHLHEYGRLLLKLATCYEVSSVDYQKLQDACSKFESMALHLKRRRKEAEYTLHFWKSFPGKMTDSLRKPSRRLICESSNKALTLQNAGRFSVNWFILFNDALVHAQGMVPYKSLFSTHHIFPLATLWVEPISEESTGVYGLKVTSPEESFALLASSPAEKAKWLRSINQAVEQALSGLGHDGIPPSTGMTQRADPPISRTASYTFYKDSRLKDAKYDGRWVSGKPHGRGVVKWPDGRMYTGTFKNGFEDGFGDYVVSNKTLNSCDHYQGQWKDGKMHGFGTFRYASGEVYEGSFQDNMRHGHGMLRSGKLNSTSPSVFIGQWQYDKKSGYGVFDDITRGEKYMGMWLDDQRQGNGVVVTQFGLYYEGAFSNNKMMGTGVLLSEDDTTFEGEFLEDWTLNGKGVLTMPNGDYIEGSFCGVWGTGLKMSGSYYKPSLYDSDKEKGHALKLGRLAVHSEEKWKAVFLECWNRLGCDSPVQGQTTTAWDNIAIALTANLRQQRDSPELLSRSHHKTLESLECIPQHVGPVTLEVYHTIRRYLVKACDTPLHPLGRLVETLVSVYRMTYVGVGANRRLLPQAVNEITSYLSRIFQLVRFLFPELPEEGGSLADPPKEKVGTDADGKLLESPKPGCVVSSSALLLPVLLPRLYPPLFTLYALEKEKEDDVYWECVLRLNKQPDLALLAFLGVQQKFWPVTVTIHGEKQQVLSSTKDACFASAVETLQQISTTFTPSDKLQVIQLTFEEITQEVLALLKQDFLWSMDDLFPVFLFVVLRARIRNLGSEVSLIEDLMDPCVQHGEHGIMFTTLKACYYQIQHEKVT

Target ORF information:

RefSeq Version	XM_009302177.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio amyotrophic lateral sclerosis 2b (juvenile) (als2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302177.3

ORF Insert Sequence:

ATGGAGAAAC TGATTAGCTC TGGTGAGGGG GACAGTTCTG GGGAGAGAGG TCTTCTGCAC 
ACGTGGAAGG GATATTCATA CAGCACCGCA CCAGAGAGGG TGCTGCTCTC CCGGCCGGTG 
CTCCAGGCTG CCCTGGGAGC TAGACATGGT GTTCTTCTAG TAGAGGGCGG ACAAGTGTAC 
AGTTTTGGTG AGTTGCCTTG GAAGCAGAAC CAAACATCAT CAGCAGAGCC CATTTTGGAG 
GCAGTGCTGA GCGAACAGCG TGTTGTTTTT GTGGCTGCTG GCTCCGCCCA CAGCGGAGTC 
GTGACGGAGG ATGGAGGAGT GCACATGTGG GGAGAAAATG TTCACGGCCA GTGTGGCTTG 
TTGGGACTGT CTGTTATCCC TAACCCCACC CCTGTGGGTG TGCTTGACTC TGAGGCCACG 
CCACCTCAGA CGGTTAAAAT CCTGGAGGTG GCATGTGGCG ATCAGCACAC CCTGGCTCTT 
TCAGCCAAGC ATGAAGTCTG GGCTTGGGGT AGCGGCTGCC AGCTGGGCCT CAACACTTCA 
ACTTTTCCCG TCTGGAAGCC CCAAAAAGTG GACCACCTAT CTGGGAGGCA TGTATTACAG 
GTGGCGTGTG GATCCGCTCA CAGCGTGGCT CTCGTACGCT GTCTCCCTCC TCCACAGGAG 
CCCCGAAAAC CCCCTCAAGA CAAATGCGGT CAGTGCCATC AGCTGCTCTA CACTATGACA 
GATAAAGAAG ACCATGTCAT TATCTCTGAC GGCCATCACT GTCCACTGGG CGTTGAGATG 
GACAGCACTG AGATCAAGTC TGCAGTGGAG GGAAGTCCAG ATCCGGGGAA ACCAGGGAAG 
AGCTCTCCTA CTGAACCTGT TCCTTGTATG TCCACCCAGA TCTCCAAATC ACCCACTTCC 
AGTAACATCT CCGAATCTAC TCGTCCTCCA GAAAGCACAC TTCCATTTGA ACTTCAAAAT 
CAGGTTCCTG CGTATTGCCA AGAATCTATT GAAGCACCTG TTGAAGACTC GGCTGCTCAT 
GAAGCTCCAG CAGCAGCTGG AGAGGTTTGT GTGGAAGCAT CTGAGAATAG CATCCCTGAG 
CCGGATAGCT GCAGATCCTC AGGGAAGTCT CCATATCCTG ATGAGCAGGC GGTGAAGGCG 
TATCTGAAGA GACTTTCTGA TCATGCTTTA GCAGAGCACA CCATCAAGAC CTCCAGCACA 
ATTCATTCAG CTCAGCTCTC GGGTGACCCT GCTAATCTGT TCGCTTCTGA GCCCTTGATC 
CCGGTTGCTC AGTCTGTGAC TCCTATGGGC TCCGCATTGA ACAACTTAGT GGTGTCGTGT 
GCCTCTGCAG TAGGGGAGCG TGTAGTCTCA ACATATGAAG CGCTCTCCCT GAGAAAAGTC 
ATTGGTTACT GGGTTCCAGG AGAGGGTCGC GAGCGAGTGG AGGAGAGACT CCGTCTGGAA 
GAGTCCATGC AGGGGAAGAA GAGCTCCAGC CTGGGGGACA TCCGAGAGGA GGAGGCAGAG 
CTCAGCCGCC GCCTGTCCCT GCCCGGCCTG CTCTCTCAGG TATCTCCTCG CCTCCTACGG 
AGAACAAGTC GCCCCCGTAT GCGGGCTGTC CCGCTCGCTT CAGGAGGTAT ACCTGAGACT 
GATGCCCACC TGCCTTCCTT ACAGACGGAA GTGTGGAGCT GGGGACAAGG CCAGGATGGA 
CAACTTGGAC ATGGAGACCT CCTTCCCAGA CTGCAGCCTG ATTGTGTAAA GAGTCTAAAT 
GGTAAGGAGG TGCTGAAAGT GGCTGCAGGG GCTCATCACT CTCTCGCTCT GACAGCACAG 
TCACAGGTTT TCTCCTGGGG TTGTAACACT TCTGGACAGC TGGGACACAT GGAATCGCCC 
ACCACCTTCC CTCAATTGAC CAAGCTGTCA GATGGGATTC GTGTGTGGGA TATTGGGGCA 
GGTCTGCAGC ACACTCTCTT ATTGGCTGAC GGAGACTGTA TTCAGCCAAT CCTGTACTAC 
AGCGGACAGC AGGTGAAGGA AGTGCCGGAG CCAACAGAGG AAGAGGAGCA GACTGCAGAA 
TACACGCAAC AACCTGTGCT GCTGCCCTTC TGTATGAACT TGGGCTATGT AAGCAGTGTG 
TATGCAGGTG GACGGACGTG TGCAGCGCTG ACGGATTGTA ATGTCATGGG TTTTATTGCT 
AGTCTGCATG AGCTGGCGGC TGCAGAGAGA AAATATTACT GCAAACTCAG CAATATCAAG 
AGTCTTCTGC TCCAGCCATT GCTCAAGCTG GATTCTCTAA GCTCTGCCCT GGGTCAGTCA 
TCTGCAGGGC TGTTGCAGAA TCTGATTGGT CGAATGAGTC GACTGTCTCA GCTTACAGGT 
CAAAACTCGG CCTCTCTGAC CTCGTTCCTG CAGCGTTCAC GAGACATCCG AGGTCTTGTT 
ATTCTTGAAG ATGCACATCT GTTCCTGGAC ACATATTCAG AGTACTCTTC TGCAGTTGGT 
GATCTTTTAG TCATGGGAGG ATTTCAAGCC CTTGTCAAAC CTTGCCATGA TGTGTTTGGG 
AAAGGAGCAG AGGTTGTTCA GAGGTTGTCT GAATGTTCTG AAGAAGGTTT GACCTTGGCA 
GATGTTCTGG CGCATCTCTT TTACCTGCCC ATAAAACACC TGCATGAATA TGGACGACTG 
CTGCTTAAAC TGGCTACCTG CTATGAGGTG AGTTCTGTGG ACTATCAGAA GCTGCAGGAT 
GCCTGCTCAA AGTTCGAATC CATGGCTCTT CATCTAAAGA GGAGGAGAAA GGAAGCTGAA 
TACACGCTCC ACTTCTGGAA GAGTTTTCCT GGAAAGATGA CCGACTCCCT GCGTAAACCG 
TCTCGTCGGC TGATCTGTGA AAGCAGTAAT AAGGCTTTGA CTCTTCAAAA CGCAGGACGT 
TTTTCCGTCA ACTGGTTCAT CCTCTTCAAC GACGCACTCG TTCATGCACA GGGTATGGTC 
CCCTATAAGA GTCTTTTTTC CACCCATCAC ATTTTTCCAT TGGCTACGCT GTGGGTGGAG 
CCAATTTCTG AGGAGAGCAC AGGCGTATAT GGCTTAAAAG TGACTTCGCC TGAGGAAAGC 
TTCGCTCTGC TGGCTTCATC ACCAGCTGAA AAAGCAAAGT GGCTTCGTTC TATAAACCAA 
GCGGTAGAGC AGGCGCTCAG TGGATTGGGG CATGATGGGA TACCTCCTTC TACAGGGATG 
ACGCAGAGAG CAGATCCACC CATCTCCCGG ACAGCATCCT ACACCTTCTA CAAAGACAGT 
CGGCTAAAGG ATGCCAAATA TGATGGCCGA TGGGTCTCAG GGAAACCCCA TGGGCGGGGC 
GTGGTCAAAT GGCCAGATGG ACGAATGTAC ACGGGGACAT TCAAGAACGG TTTTGAAGAT 
GGTTTTGGAG ATTACGTTGT GTCCAATAAA ACCCTGAACA GCTGTGATCA TTATCAAGGC 
CAGTGGAAAG ATGGCAAGAT GCACGGTTTT GGAACATTCA GATATGCCTC TGGTGAAGTG 
TACGAAGGCT CATTTCAAGA CAACATGCGT CATGGTCATG GGATGCTGCG TAGTGGGAAG 
CTGAATTCCA CCTCTCCCAG TGTCTTTATC GGCCAGTGGC AGTACGACAA AAAGTCTGGC 
TATGGAGTTT TTGATGACAT CACACGAGGA GAGAAGTATA TGGGCATGTG GCTGGATGAT 
CAACGGCAGG GGAACGGAGT TGTTGTTACA CAATTTGGCC TATACTATGA AGGTGCATTC 
AGCAACAACA AAATGATGGG TACTGGGGTG TTACTCTCTG AAGATGACAC TACATTTGAA 
GGAGAGTTTT TAGAAGACTG GACTCTTAAT GGAAAGGGTG TGTTGACCAT GCCGAATGGA 
GACTATATTG AAGGCTCTTT TTGTGGAGTT TGGGGAACCG GGCTAAAGAT GTCAGGTTCC 
TACTACAAAC CCAGCCTGTA CGACTCAGAC AAGGAGAAGG GTCATGCACT TAAACTGGGC 
CGGTTAGCAG TTCACTCTGA AGAGAAGTGG AAGGCTGTGT TTTTGGAGTG CTGGAACAGG 
CTCGGTTGTG ATTCCCCTGT ACAGGGGCAA ACCACGACAG CGTGGGACAA CATTGCCATT 
GCACTCACTG CTAACCTGAG ACAGCAGAGA GACAGTCCAG AGTTGCTGAG CCGCTCACAT 
CACAAAACAC TAGAGAGTCT TGAGTGCATC CCTCAACATG TGGGTCCTGT AACATTAGAG 
GTGTACCACA CGATCCGTCG GTACCTGGTG AAGGCATGTG ATACTCCTCT GCATCCTCTG 
GGTCGGCTGG TGGAGACGCT GGTTTCGGTA TACCGGATGA CGTATGTTGG TGTTGGAGCA 
AACCGCAGGT TATTACCGCA GGCCGTCAAT GAGATCACGT CCTATCTCAG CCGTATCTTC 
CAGCTCGTCA GATTTCTCTT TCCCGAACTG CCAGAAGAAG GAGGTTCACT AGCCGACCCT 
CCAAAAGAGA AGGTCGGCAC AGATGCTGAT GGAAAGCTGC TGGAGTCTCC TAAACCTGGG 
TGTGTGGTCA GCAGCTCTGC TCTCCTCCTG CCGGTGCTGT TGCCACGTCT CTATCCTCCT 
CTCTTCACGC TCTACGCTCT AGAGAAAGAG AAAGAGGACG ATGTGTACTG GGAGTGTGTG 
CTGCGACTCA ACAAACAGCC AGATCTGGCA CTGCTGGCTT TCCTCGGGGT CCAACAGAAG 
TTCTGGCCTG TAACTGTTAC AATACATGGA GAAAAACAAC AGGTTCTCTC AAGCACTAAA 
GACGCCTGTT TCGCCTCAGC AGTTGAGACT TTGCAGCAAA TAAGCACAAC GTTCACACCC 
TCAGACAAGC TTCAGGTGAT CCAGCTGACC TTCGAGGAAA TAACCCAGGA AGTGCTCGCA 
CTCCTGAAAC AGGATTTCTT GTGGTCTATG GATGACCTGT TTCCTGTCTT CCTCTTTGTT 
GTTCTGCGTG CACGGATAAG GAATCTGGGG TCAGAGGTTA GTCTCATTGA GGACCTAATG 
GATCCATGTG TACAACATGG AGAGCATGGC ATCATGTTCA CCACCCTCAA GGCGTGCTAC 
TATCAAATCC AGCATGAGAA GGTCACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	als2b
Entrez Gene ID	100318899
Full Name	amyotrophic lateral sclerosis 2b (juvenile)
Synonyms	wu:fi21b09
General protein information	Preferred Names amyotrophic lateral sclerosis 2b (juvenile) Names alsin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Canis lupus familiaris (dog)	ALS2	XP_003640234.1
Danio rerio (zebrafish)	als2b	XP_002662342.2
Homo sapiens (human)	ALS2	NP_065970.2
Bos taurus (cattle)	ALS2	XP_005202742.1
Rattus norvegicus (Norway rat)	Als2	NP_001013431.1
Pan troglodytes (chimpanzee)	ALS2	NP_001073389.1
Xenopus tropicalis (tropical clawed frog)	als2	XP_004917813.1
Macaca mulatta (Rhesus monkey)	ALS2	XP_001091562.2
Mus musculus (house mouse)	Als2	NP_001153420.2
Gallus gallus (chicken)	ALS2	XP_421940.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

als2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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