Polr3b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polr3b
Entrez Gene ID	70428
Full Name	polymerase (RNA) III (DNA directed) polypeptide B
Synonyms	2700078H01Rik,A330032P03Rik,C85372,RPC2
General protein information	Preferred Names polymerase (RNA) III (DNA directed) polypeptide B Names DNA-directed RNA polymerase III subunit RPC2 C128 DNA-directed RNA polymerase III 127.6 kDa polypeptide DNA-directed RNA polymerase III B DNA-directed RNA polymerase III subunit B RNA polymerase III subunit C2 RNA polymerase III subunit RPC2 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_027423.1	NP_081699.1	DNA-directed RNA polymerase III subunit RPC2
NM_027423.2	NP_081699.1	DNA-directed RNA polymerase III subunit RPC2

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F01078g	XP_455128.1
Schizosaccharomyces pombe (fission yeast)	rpc2	NP_593690.1
Drosophila melanogaster (fruit fly)	RpIII128	NP_523706.1
Mus musculus (house mouse)	Polr3b	NP_081699.1
Rattus norvegicus (Norway rat)	Polr3b	NP_001178807.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008151	XP_317312.4
Oryza sativa (rice)	Os03g0403100	NP_001050323.1
Neurospora crassa	NCU01772	XP_957195.1
Homo sapiens (human)	POLR3B	NP_060552.4
Gallus gallus (chicken)	POLR3B	XP_416307.3
Danio rerio (zebrafish)	polr3b	XP_690811.2
Magnaporthe oryzae (rice blast fungus)	MGG_06984	XP_003709735.1
Macaca mulatta (Rhesus monkey)	POLR3B	XP_001099515.1
Xenopus tropicalis (tropical clawed frog)	polr3b	NP_001121521.1
Pan troglodytes (chimpanzee)	POLR3B	XP_001161567.1
Canis lupus familiaris (dog)	POLR3B	XP_531760.2
Bos taurus (cattle)	POLR3B	NP_001179718.1
Caenorhabditis elegans (roundworm)	F09F7.3	NP_498192.2
Saccharomyces cerevisiae (baker's yeast)	RET1	NP_014850.1
Arabidopsis thaliana (thale cress)	NRPC2	NP_199327.4
Eremothecium gossypii	AGOS_ADL275C	NP_983821.2

Gene Ontology
Bibliography

Related articles in PubMed

The leukodystrophy mutation Polr3b R103H causes homozygote mouse embryonic lethality and impairs RNA polymerase III biogenesis.
Choquet K, Pinard M, Yang S, Moir RD, Poitras C, Dicaire MJ, Sgarioto N, Larivière R, Kleinman CL, Willis IM, Gauthier MS, Coulombe B, Brais B
Molecular brain12(1)59(2019 06)

Association of brain immune genes with social behavior of inbred mouse strains.
Ma L, Piirainen S, Kulesskaya N, Rauvala H, Tian L
Journal of neuroinflammation1275(2015 Apr)

Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and disrupts zebrafish digestive development.
Yee NS, Gong W, Huang Y, Lorent K, Dolan AC, Maraia RJ, Pack M
PLoS biology5(11)e312(2007 Nov)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Polr3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu04189	NM_027423.2 Latest version!	Mus musculus polymerase (RNA) III (DNA directed) polypeptide B (Polr3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$664.30 $949.00
View Old Accession Versions >>
OMu04189	NM_027423.1	Mus musculus polymerase (RNA) III (DNA directed) polypeptide B (Polr3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$474.50-$664.30 $949.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polr3b ( NM_027423.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081699.1 Mus musculus polymerase (RNA) III (DNA directed) polypeptide B (Polr3b), mRNA.

Price & Availability↑

CloneID	OMu04189
Clone ID Related Accession (Same CDS sequence)	NM_027423.1 , NM_027423.2
Accession Version	NM_027423.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509120000000
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase III subunit RPC2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC068143.1. On Apr 7, 2004 this sequence version replaced XM_125795.2. ##Evidence-Data-START## Transcript exon combination :: BC068143.1, SRR1660823.184404.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGATGTTC TAGCCGAGGA GTTCGGGAGC CTGACTCCGG AGCAGCTGAC TGCGCCGATC 
CCGACTGTGG AGGAAAAATG GAGGCTGCTT CCAGCATTTT TAAAGGTTAA AGGCCTGGTG 
AAACAGCACA TAGACTCCTT TAACTATTTC ATTAATGTAG AGATAAAGAA GATAATGAAA 
GCCAATGAAA AGGTTACAAG TGATGCTGAC CCCATGTGGT ACTTAAAATA CCTTAATATC 
TATGTCGGAC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT GTCCCCTCAT 
GAGTGCCGTC TGAGGGACAT GACGTACTCC GCCCCAATCA CAGTGGACAT TGAGTATACC 
CGAGGCAGCC AGAGGATAAT CCGCAACGCC TTACCCATTG GCAGAATGCC CATCATGCTC 
CGAAGCTCGA ACTGTGTTCT AACAGGGAAA ACGCCGGCAG AGTTTGCCAA ACTGAATGAG 
TGTCCACTAG ACCCAGGTGG CTACTTCATT GTTAAAGGAG TGGAAAAAGT CATTCTTATC 
CAAGAGCAGC TGTCCAAGAA CAGGATCATT GTGGAGGCCG ACAGGAAAGG AGCCGTTGGA 
GCCTCAGTTA CCAGCTCCAC CCATGAGAAG AAAAGCAGAA CCAACATGGC CGTGAAACAG 
GGTCGATTCT ATCTGAGGCA CAACACCCTG TCTGAAGATA TCCCCATTGT CATCATATTT 
AAGGCCATGG GTGTGGAGAG TGACCAGGAA ATCGTGCAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGCTTGGAG GAGTGCCAGA AAGCTCAGAT CTTCACCCAG 
ATGCAGGCAT TGAAGTATAT AGGGAATAAA GTAAGAAGAC AAAGAATGTG GGGAGGTGGA 
CCAAAGAAAA CCAAAATAGA AGAAGCAAGA GAACTCCTGG CTTCTACCAT TCTGACCCAC 
GTACCAGTGA AGGAATTCAA TTTCCGAGCT AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGGGTGATCC TGGCCCAAGG AGATAACAAG GTCGATGACA GAGACTATTA TGGCAACAAA 
CGACTGGAAT TGGCAGGACA GCTCTTATCT CTTCTTTTTG AAGATTTGTT TAAAAAGTTT 
AATTCAGAAA TGAAGAAGAT TGCAGACCAA GTGATTCCTA AACAGAGAGC AGCCCAGTTT 
GACGTCGTTA AACACATGCG CCAAGACCAG ATTACCAACG GCATGGTCAA TGCCATCTCC 
ACGGGAAATT GGTCTCTGAA GAGATTTAAG ATGGACCGAC AGGGTGTGAC CCAGGTGCTG 
TCTCGCTTGT CATATATATC TGCACTCGGC ATGATGACAA GAATCTCTTC CCAGTTTGAA 
AAGACGAGGA AAGTGAGTGG CCCTCGTTCT CTGCAGCCGT CTCAGTGGGG AATGCTCTGT 
CCCTCCGACA CACCTGAAGG GGAGGCCTGT GGTTTGGTTA AGAACTTGGC CCTCATGACA 
CATATAACAA CTGACATGGA AGATGGACCC ATTATTAAGT TAGCTGGGAA CTTGGGAGTA 
GAAGATGTGA ATTTATTATG TGGAGAAGAG CTCTCTTACC CAAATGTGTT TCTTGTCTTC 
CTGAATGGTA ACATCCTTGG TGTCATTCGA GACCATAAAA AGCTTGTCAG CACATTCCGG 
CTGATGCGAA GAGCAGGCTA TATCAATGAA TTTGTTTCTA TCTCCACAAA CCTTACGGAT 
CGCTGTGTTT ATATTTCCTC TGATGGCGGG CGCTTGTGCA GGCCTTACAT AATTGTTAAG 
AAACAAAAGC CAGCTGTCAC AAATAAGCAC ATGGAAGAGT TGGCTCAAGG CTACAGGAAT 
TTTGAAGATT TCTTACATGA AAGTCTAGTT GAATACTTAG ATGTGAATGA AGAAAATGAC 
TGTAACATTG CGCTCTACGA ACATACAATT AATAAAGACA CCACCCACTT GGAGATTGAA 
CCCTTCACTC TGCTTGGTGT TTGTGCCGGC CTGATCCCAT ACCCTCATCA CAACCAGTCT 
CCAAGAAACA CCTACCAGTG TGCTATGGGC AAGCAAGCCA TGGGTACCAT TGGCTACAAT 
CAACGGAACA GAATTGATAC ACTTATGTAT CTACTAGCAT ATCCACAAAA GCCCATGGTT 
AAAACAAAAA CAATCGAGTT GATAGATTTT GAGAAGTTGC CGGCTGGCCA GAACGCAACT 
GTTGCCGTGA TGAGCTACAG CGGCTATGAC ATTGAGGACG CGCTGGTTTT GAACAAGGCC 
TCTCTGGACA GAGGTTTTGG GCGTTGTCTT GTGTATAAAA ATGCTAAGTG CACCTTGAAA 
CGATACACCA ATCAGACCTT TGATAAGGTG ATGGGTCCCA TGTTGGATGC TGCTACCAGA 
AAACCCATCT GGCGACATGA AATTTTAGAT GCAGATGGCA TTTGTTCTCC AGGTGAGAAA 
GTGGAAAACA AACAGGTGCT TGTAAACAAG TCCATGCCCA CAGTGACACA GATTCCTTTG 
GAAGGGAGCA ACGTGCCACA GCAACCACAG TATAAAGATG TGCCTATCAC GTACAAAGGT 
GCGACAGACT CCTACATCGA GAAAGTGATG ATCTCTTCCA ACGCCGAGGA TGCTTTCCTC 
ATCAAGATGC TCCTGAGACA GACGCGGCGC CCAGAGATTG GAGACAAATT CAGCAGTCGC 
CATGGACAAA AGGGTGTCTG TGGCCTGATC GTCCCCCAGG AAGACATGCC GTTCTGTGAT 
TCTGGCATCT GTCCCGACAT CATAATGAAC CCACACGGCT TCCCATCAAG AATGACGGTT 
GGAAAGTTAA TTGAGCTGCT GGCTGGCAAG GCAGGCGTGT TAGACGGCAG GTTCCACTAC 
GGCACTGCCT TTGGAGGGAG TAAAGTGAAG GATGTGTGTG AAGATCTCGT TCGCCATGGT 
TACAACTACC TGGGGAAGGA CTACGTCACC TCAGGCATCA CAGGTGAGCC CTTAGAAGCA 
TACATCTATT TTGGACCTGT GTATTATCAG AAACTGAAAC ACATGGTGCT GGATAAAATG 
CACGCCCGGG CCCGAGGACC ACGAGCTGTC CTTACCAGGC AGCCCACTGA AGGGCGGTCG 
CGCGATGGTG GCTTGCGACT TGGAGAAATG GAACGCGACT GTCTAATCGG CTATGGAGCG 
AGTATGCTTT TGCTGGAGAG ACTAATGATC TCAAGTGATG CCTTTGAGGT TGACGTCTGC 
GGGCAGTGTG GACTCCTGGG ATATTCTGGC TGGTGCCACT ACTGCAAATC TTCGTGCCAC 
GTGTCCTCCC TCCGCATCCC GTATGCCTGC AAGCTGCTCT TCCAGGAGTT ACAGTCCATG 
AACATCATCC CCAGGCTGAA ACTGGCCAAG TACAACGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081699.1
CDS	31..3432
Translation	MDVLAEEFGSLTPEQLTAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGAVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIIKLAGNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVSTFRLMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIDFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHGYNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLAKYNE

Target ORF information:

RefSeq Version	NM_027423.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) III (DNA directed) polypeptide B (Polr3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027423.1

ORF Insert Sequence:

ATGGATGTTC TAGCCGAGGA GTTCGGGAGC CTGACTCCGG AGCAGCTGAC TGCGCCGATC 
CCGACTGTGG AGGAAAAATG GAGGCTGCTT CCAGCATTTT TAAAGGTTAA AGGCCTGGTG 
AAACAGCACA TAGACTCCTT TAACTATTTC ATTAATGTAG AGATAAAGAA GATAATGAAA 
GCCAATGAAA AGGTTACAAG TGATGCTGAC CCCATGTGGT ACTTAAAATA CCTTAATATC 
TATGTCGGAC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT GTCCCCTCAT 
GAGTGCCGTC TGAGGGACAT GACGTACTCC GCCCCAATCA CAGTGGACAT TGAGTATACC 
CGAGGCAGCC AGAGGATAAT CCGCAACGCC TTACCCATTG GCAGAATGCC CATCATGCTC 
CGAAGCTCGA ACTGTGTTCT AACAGGGAAA ACGCCGGCAG AGTTTGCCAA ACTGAATGAG 
TGTCCACTAG ACCCAGGTGG CTACTTCATT GTTAAAGGAG TGGAAAAAGT CATTCTTATC 
CAAGAGCAGC TGTCCAAGAA CAGGATCATT GTGGAGGCCG ACAGGAAAGG AGCCGTTGGA 
GCCTCAGTTA CCAGCTCCAC CCATGAGAAG AAAAGCAGAA CCAACATGGC CGTGAAACAG 
GGTCGATTCT ATCTGAGGCA CAACACCCTG TCTGAAGATA TCCCCATTGT CATCATATTT 
AAGGCCATGG GTGTGGAGAG TGACCAGGAA ATCGTGCAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGCTTGGAG GAGTGCCAGA AAGCTCAGAT CTTCACCCAG 
ATGCAGGCAT TGAAGTATAT AGGGAATAAA GTAAGAAGAC AAAGAATGTG GGGAGGTGGA 
CCAAAGAAAA CCAAAATAGA AGAAGCAAGA GAACTCCTGG CTTCTACCAT TCTGACCCAC 
GTACCAGTGA AGGAATTCAA TTTCCGAGCT AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGGGTGATCC TGGCCCAAGG AGATAACAAG GTCGATGACA GAGACTATTA TGGCAACAAA 
CGACTGGAAT TGGCAGGACA GCTCTTATCT CTTCTTTTTG AAGATTTGTT TAAAAAGTTT 
AATTCAGAAA TGAAGAAGAT TGCAGACCAA GTGATTCCTA AACAGAGAGC AGCCCAGTTT 
GACGTCGTTA AACACATGCG CCAAGACCAG ATTACCAACG GCATGGTCAA TGCCATCTCC 
ACGGGAAATT GGTCTCTGAA GAGATTTAAG ATGGACCGAC AGGGTGTGAC CCAGGTGCTG 
TCTCGCTTGT CATATATATC TGCACTCGGC ATGATGACAA GAATCTCTTC CCAGTTTGAA 
AAGACGAGGA AAGTGAGTGG CCCTCGTTCT CTGCAGCCGT CTCAGTGGGG AATGCTCTGT 
CCCTCCGACA CACCTGAAGG GGAGGCCTGT GGTTTGGTTA AGAACTTGGC CCTCATGACA 
CATATAACAA CTGACATGGA AGATGGACCC ATTATTAAGT TAGCTGGGAA CTTGGGAGTA 
GAAGATGTGA ATTTATTATG TGGAGAAGAG CTCTCTTACC CAAATGTGTT TCTTGTCTTC 
CTGAATGGTA ACATCCTTGG TGTCATTCGA GACCATAAAA AGCTTGTCAG CACATTCCGG 
CTGATGCGAA GAGCAGGCTA TATCAATGAA TTTGTTTCTA TCTCCACAAA CCTTACGGAT 
CGCTGTGTTT ATATTTCCTC TGATGGCGGG CGCTTGTGCA GGCCTTACAT AATTGTTAAG 
AAACAAAAGC CAGCTGTCAC AAATAAGCAC ATGGAAGAGT TGGCTCAAGG CTACAGGAAT 
TTTGAAGATT TCTTACATGA AAGTCTAGTT GAATACTTAG ATGTGAATGA AGAAAATGAC 
TGTAACATTG CGCTCTACGA ACATACAATT AATAAAGACA CCACCCACTT GGAGATTGAA 
CCCTTCACTC TGCTTGGTGT TTGTGCCGGC CTGATCCCAT ACCCTCATCA CAACCAGTCT 
CCAAGAAACA CCTACCAGTG TGCTATGGGC AAGCAAGCCA TGGGTACCAT TGGCTACAAT 
CAACGGAACA GAATTGATAC ACTTATGTAT CTACTAGCAT ATCCACAAAA GCCCATGGTT 
AAAACAAAAA CAATCGAGTT GATAGATTTT GAGAAGTTGC CGGCTGGCCA GAACGCAACT 
GTTGCCGTGA TGAGCTACAG CGGCTATGAC ATTGAGGACG CGCTGGTTTT GAACAAGGCC 
TCTCTGGACA GAGGTTTTGG GCGTTGTCTT GTGTATAAAA ATGCTAAGTG CACCTTGAAA 
CGATACACCA ATCAGACCTT TGATAAGGTG ATGGGTCCCA TGTTGGATGC TGCTACCAGA 
AAACCCATCT GGCGACATGA AATTTTAGAT GCAGATGGCA TTTGTTCTCC AGGTGAGAAA 
GTGGAAAACA AACAGGTGCT TGTAAACAAG TCCATGCCCA CAGTGACACA GATTCCTTTG 
GAAGGGAGCA ACGTGCCACA GCAACCACAG TATAAAGATG TGCCTATCAC GTACAAAGGT 
GCGACAGACT CCTACATCGA GAAAGTGATG ATCTCTTCCA ACGCCGAGGA TGCTTTCCTC 
ATCAAGATGC TCCTGAGACA GACGCGGCGC CCAGAGATTG GAGACAAATT CAGCAGTCGC 
CATGGACAAA AGGGTGTCTG TGGCCTGATC GTCCCCCAGG AAGACATGCC GTTCTGTGAT 
TCTGGCATCT GTCCCGACAT CATAATGAAC CCACACGGCT TCCCATCAAG AATGACGGTT 
GGAAAGTTAA TTGAGCTGCT GGCTGGCAAG GCAGGCGTGT TAGACGGCAG GTTCCACTAC 
GGCACTGCCT TTGGAGGGAG TAAAGTGAAG GATGTGTGTG AAGATCTCGT TCGCCATGGT 
TACAACTACC TGGGGAAGGA CTACGTCACC TCAGGCATCA CAGGTGAGCC CTTAGAAGCA 
TACATCTATT TTGGACCTGT GTATTATCAG AAACTGAAAC ACATGGTGCT GGATAAAATG 
CACGCCCGGG CCCGAGGACC ACGAGCTGTC CTTACCAGGC AGCCCACTGA AGGGCGGTCG 
CGCGATGGTG GCTTGCGACT TGGAGAAATG GAACGCGACT GTCTAATCGG CTATGGAGCG 
AGTATGCTTT TGCTGGAGAG ACTAATGATC TCAAGTGATG CCTTTGAGGT TGACGTCTGC 
GGGCAGTGTG GACTCCTGGG ATATTCTGGC TGGTGCCACT ACTGCAAATC TTCGTGCCAC 
GTGTCCTCCC TCCGCATCCC GTATGCCTGC AAGCTGCTCT TCCAGGAGTT ACAGTCCATG 
AACATCATCC CCAGGCTGAA ACTGGCCAAG TACAACGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polr3b ( NM_027423.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081699.1 Mus musculus polymerase (RNA) III (DNA directed) polypeptide B (Polr3b), mRNA.

Price & Availability↑

CloneID	OMu04189
Clone ID Related Accession (Same CDS sequence)	NM_027423.1 , NM_027423.2
Accession Version	NM_027423.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571760000000
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase III subunit RPC2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK165886.1 and BC068143.1. On Jun 28, 2018 this sequence version replaced NM_027423.1. ##Evidence-Data-START## Transcript exon combination :: BC068143.1, SRR1660825.184404.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGTTC TAGCCGAGGA GTTCGGGAGC CTGACTCCGG AGCAGCTGAC TGCGCCGATC 
CCGACTGTGG AGGAAAAATG GAGGCTGCTT CCAGCATTTT TAAAGGTTAA AGGCCTGGTG 
AAACAGCACA TAGACTCCTT TAACTATTTC ATTAATGTAG AGATAAAGAA GATAATGAAA 
GCCAATGAAA AGGTTACAAG TGATGCTGAC CCCATGTGGT ACTTAAAATA CCTTAATATC 
TATGTCGGAC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT GTCCCCTCAT 
GAGTGCCGTC TGAGGGACAT GACGTACTCC GCCCCAATCA CAGTGGACAT TGAGTATACC 
CGAGGCAGCC AGAGGATAAT CCGCAACGCC TTACCCATTG GCAGAATGCC CATCATGCTC 
CGAAGCTCGA ACTGTGTTCT AACAGGGAAA ACGCCGGCAG AGTTTGCCAA ACTGAATGAG 
TGTCCACTAG ACCCAGGTGG CTACTTCATT GTTAAAGGAG TGGAAAAAGT CATTCTTATC 
CAAGAGCAGC TGTCCAAGAA CAGGATCATT GTGGAGGCCG ACAGGAAAGG AGCCGTTGGA 
GCCTCAGTTA CCAGCTCCAC CCATGAGAAG AAAAGCAGAA CCAACATGGC CGTGAAACAG 
GGTCGATTCT ATCTGAGGCA CAACACCCTG TCTGAAGATA TCCCCATTGT CATCATATTT 
AAGGCCATGG GTGTGGAGAG TGACCAGGAA ATCGTGCAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGCTTGGAG GAGTGCCAGA AAGCTCAGAT CTTCACCCAG 
ATGCAGGCAT TGAAGTATAT AGGGAATAAA GTAAGAAGAC AAAGAATGTG GGGAGGTGGA 
CCAAAGAAAA CCAAAATAGA AGAAGCAAGA GAACTCCTGG CTTCTACCAT TCTGACCCAC 
GTACCAGTGA AGGAATTCAA TTTCCGAGCT AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGGGTGATCC TGGCCCAAGG AGATAACAAG GTCGATGACA GAGACTATTA TGGCAACAAA 
CGACTGGAAT TGGCAGGACA GCTCTTATCT CTTCTTTTTG AAGATTTGTT TAAAAAGTTT 
AATTCAGAAA TGAAGAAGAT TGCAGACCAA GTGATTCCTA AACAGAGAGC AGCCCAGTTT 
GACGTCGTTA AACACATGCG CCAAGACCAG ATTACCAACG GCATGGTCAA TGCCATCTCC 
ACGGGAAATT GGTCTCTGAA GAGATTTAAG ATGGACCGAC AGGGTGTGAC CCAGGTGCTG 
TCTCGCTTGT CATATATATC TGCACTCGGC ATGATGACAA GAATCTCTTC CCAGTTTGAA 
AAGACGAGGA AAGTGAGTGG CCCTCGTTCT CTGCAGCCGT CTCAGTGGGG AATGCTCTGT 
CCCTCCGACA CACCTGAAGG GGAGGCCTGT GGTTTGGTTA AGAACTTGGC CCTCATGACA 
CATATAACAA CTGACATGGA AGATGGACCC ATTATTAAGT TAGCTGGGAA CTTGGGAGTA 
GAAGATGTGA ATTTATTATG TGGAGAAGAG CTCTCTTACC CAAATGTGTT TCTTGTCTTC 
CTGAATGGTA ACATCCTTGG TGTCATTCGA GACCATAAAA AGCTTGTCAG CACATTCCGG 
CTGATGCGAA GAGCAGGCTA TATCAATGAA TTTGTTTCTA TCTCCACAAA CCTTACGGAT 
CGCTGTGTTT ATATTTCCTC TGATGGCGGG CGCTTGTGCA GGCCTTACAT AATTGTTAAG 
AAACAAAAGC CAGCTGTCAC AAATAAGCAC ATGGAAGAGT TGGCTCAAGG CTACAGGAAT 
TTTGAAGATT TCTTACATGA AAGTCTAGTT GAATACTTAG ATGTGAATGA AGAAAATGAC 
TGTAACATTG CGCTCTACGA ACATACAATT AATAAAGACA CCACCCACTT GGAGATTGAA 
CCCTTCACTC TGCTTGGTGT TTGTGCCGGC CTGATCCCAT ACCCTCATCA CAACCAGTCT 
CCAAGAAACA CCTACCAGTG TGCTATGGGC AAGCAAGCCA TGGGTACCAT TGGCTACAAT 
CAACGGAACA GAATTGATAC ACTTATGTAT CTACTAGCAT ATCCACAAAA GCCCATGGTT 
AAAACAAAAA CAATCGAGTT GATAGATTTT GAGAAGTTGC CGGCTGGCCA GAACGCAACT 
GTTGCCGTGA TGAGCTACAG CGGCTATGAC ATTGAGGACG CGCTGGTTTT GAACAAGGCC 
TCTCTGGACA GAGGTTTTGG GCGTTGTCTT GTGTATAAAA ATGCTAAGTG CACCTTGAAA 
CGATACACCA ATCAGACCTT TGATAAGGTG ATGGGTCCCA TGTTGGATGC TGCTACCAGA 
AAACCCATCT GGCGACATGA AATTTTAGAT GCAGATGGCA TTTGTTCTCC AGGTGAGAAA 
GTGGAAAACA AACAGGTGCT TGTAAACAAG TCCATGCCCA CAGTGACACA GATTCCTTTG 
GAAGGGAGCA ACGTGCCACA GCAACCACAG TATAAAGATG TGCCTATCAC GTACAAAGGT 
GCGACAGACT CCTACATCGA GAAAGTGATG ATCTCTTCCA ACGCCGAGGA TGCTTTCCTC 
ATCAAGATGC TCCTGAGACA GACGCGGCGC CCAGAGATTG GAGACAAATT CAGCAGTCGC 
CATGGACAAA AGGGTGTCTG TGGCCTGATC GTCCCCCAGG AAGACATGCC GTTCTGTGAT 
TCTGGCATCT GTCCCGACAT CATAATGAAC CCACACGGCT TCCCATCAAG AATGACGGTT 
GGAAAGTTAA TTGAGCTGCT GGCTGGCAAG GCAGGCGTGT TAGACGGCAG GTTCCACTAC 
GGCACTGCCT TTGGAGGGAG TAAAGTGAAG GATGTGTGTG AAGATCTCGT TCGCCATGGT 
TACAACTACC TGGGGAAGGA CTACGTCACC TCAGGCATCA CAGGTGAGCC CTTAGAAGCA 
TACATCTATT TTGGACCTGT GTATTATCAG AAACTGAAAC ACATGGTGCT GGATAAAATG 
CACGCCCGGG CCCGAGGACC ACGAGCTGTC CTTACCAGGC AGCCCACTGA AGGGCGGTCG 
CGCGATGGTG GCTTGCGACT TGGAGAAATG GAACGCGACT GTCTAATCGG CTATGGAGCG 
AGTATGCTTT TGCTGGAGAG ACTAATGATC TCAAGTGATG CCTTTGAGGT TGACGTCTGC 
GGGCAGTGTG GACTCCTGGG ATATTCTGGC TGGTGCCACT ACTGCAAATC TTCGTGCCAC 
GTGTCCTCCC TCCGCATCCC GTATGCCTGC AAGCTGCTCT TCCAGGAGTT ACAGTCCATG 
AACATCATCC CCAGGCTGAA ACTGGCCAAG TACAACGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081699.1
CDS	170..3571
Translation	MDVLAEEFGSLTPEQLTAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGAVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIIKLAGNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVSTFRLMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIDFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHGYNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLAKYNE

Target ORF information:

RefSeq Version	NM_027423.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) III (DNA directed) polypeptide B (Polr3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027423.2

ORF Insert Sequence:

ATGGATGTTC TAGCCGAGGA GTTCGGGAGC CTGACTCCGG AGCAGCTGAC TGCGCCGATC 
CCGACTGTGG AGGAAAAATG GAGGCTGCTT CCAGCATTTT TAAAGGTTAA AGGCCTGGTG 
AAACAGCACA TAGACTCCTT TAACTATTTC ATTAATGTAG AGATAAAGAA GATAATGAAA 
GCCAATGAAA AGGTTACAAG TGATGCTGAC CCCATGTGGT ACTTAAAATA CCTTAATATC 
TATGTCGGAC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT GTCCCCTCAT 
GAGTGCCGTC TGAGGGACAT GACGTACTCC GCCCCAATCA CAGTGGACAT TGAGTATACC 
CGAGGCAGCC AGAGGATAAT CCGCAACGCC TTACCCATTG GCAGAATGCC CATCATGCTC 
CGAAGCTCGA ACTGTGTTCT AACAGGGAAA ACGCCGGCAG AGTTTGCCAA ACTGAATGAG 
TGTCCACTAG ACCCAGGTGG CTACTTCATT GTTAAAGGAG TGGAAAAAGT CATTCTTATC 
CAAGAGCAGC TGTCCAAGAA CAGGATCATT GTGGAGGCCG ACAGGAAAGG AGCCGTTGGA 
GCCTCAGTTA CCAGCTCCAC CCATGAGAAG AAAAGCAGAA CCAACATGGC CGTGAAACAG 
GGTCGATTCT ATCTGAGGCA CAACACCCTG TCTGAAGATA TCCCCATTGT CATCATATTT 
AAGGCCATGG GTGTGGAGAG TGACCAGGAA ATCGTGCAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGCTTGGAG GAGTGCCAGA AAGCTCAGAT CTTCACCCAG 
ATGCAGGCAT TGAAGTATAT AGGGAATAAA GTAAGAAGAC AAAGAATGTG GGGAGGTGGA 
CCAAAGAAAA CCAAAATAGA AGAAGCAAGA GAACTCCTGG CTTCTACCAT TCTGACCCAC 
GTACCAGTGA AGGAATTCAA TTTCCGAGCT AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGGGTGATCC TGGCCCAAGG AGATAACAAG GTCGATGACA GAGACTATTA TGGCAACAAA 
CGACTGGAAT TGGCAGGACA GCTCTTATCT CTTCTTTTTG AAGATTTGTT TAAAAAGTTT 
AATTCAGAAA TGAAGAAGAT TGCAGACCAA GTGATTCCTA AACAGAGAGC AGCCCAGTTT 
GACGTCGTTA AACACATGCG CCAAGACCAG ATTACCAACG GCATGGTCAA TGCCATCTCC 
ACGGGAAATT GGTCTCTGAA GAGATTTAAG ATGGACCGAC AGGGTGTGAC CCAGGTGCTG 
TCTCGCTTGT CATATATATC TGCACTCGGC ATGATGACAA GAATCTCTTC CCAGTTTGAA 
AAGACGAGGA AAGTGAGTGG CCCTCGTTCT CTGCAGCCGT CTCAGTGGGG AATGCTCTGT 
CCCTCCGACA CACCTGAAGG GGAGGCCTGT GGTTTGGTTA AGAACTTGGC CCTCATGACA 
CATATAACAA CTGACATGGA AGATGGACCC ATTATTAAGT TAGCTGGGAA CTTGGGAGTA 
GAAGATGTGA ATTTATTATG TGGAGAAGAG CTCTCTTACC CAAATGTGTT TCTTGTCTTC 
CTGAATGGTA ACATCCTTGG TGTCATTCGA GACCATAAAA AGCTTGTCAG CACATTCCGG 
CTGATGCGAA GAGCAGGCTA TATCAATGAA TTTGTTTCTA TCTCCACAAA CCTTACGGAT 
CGCTGTGTTT ATATTTCCTC TGATGGCGGG CGCTTGTGCA GGCCTTACAT AATTGTTAAG 
AAACAAAAGC CAGCTGTCAC AAATAAGCAC ATGGAAGAGT TGGCTCAAGG CTACAGGAAT 
TTTGAAGATT TCTTACATGA AAGTCTAGTT GAATACTTAG ATGTGAATGA AGAAAATGAC 
TGTAACATTG CGCTCTACGA ACATACAATT AATAAAGACA CCACCCACTT GGAGATTGAA 
CCCTTCACTC TGCTTGGTGT TTGTGCCGGC CTGATCCCAT ACCCTCATCA CAACCAGTCT 
CCAAGAAACA CCTACCAGTG TGCTATGGGC AAGCAAGCCA TGGGTACCAT TGGCTACAAT 
CAACGGAACA GAATTGATAC ACTTATGTAT CTACTAGCAT ATCCACAAAA GCCCATGGTT 
AAAACAAAAA CAATCGAGTT GATAGATTTT GAGAAGTTGC CGGCTGGCCA GAACGCAACT 
GTTGCCGTGA TGAGCTACAG CGGCTATGAC ATTGAGGACG CGCTGGTTTT GAACAAGGCC 
TCTCTGGACA GAGGTTTTGG GCGTTGTCTT GTGTATAAAA ATGCTAAGTG CACCTTGAAA 
CGATACACCA ATCAGACCTT TGATAAGGTG ATGGGTCCCA TGTTGGATGC TGCTACCAGA 
AAACCCATCT GGCGACATGA AATTTTAGAT GCAGATGGCA TTTGTTCTCC AGGTGAGAAA 
GTGGAAAACA AACAGGTGCT TGTAAACAAG TCCATGCCCA CAGTGACACA GATTCCTTTG 
GAAGGGAGCA ACGTGCCACA GCAACCACAG TATAAAGATG TGCCTATCAC GTACAAAGGT 
GCGACAGACT CCTACATCGA GAAAGTGATG ATCTCTTCCA ACGCCGAGGA TGCTTTCCTC 
ATCAAGATGC TCCTGAGACA GACGCGGCGC CCAGAGATTG GAGACAAATT CAGCAGTCGC 
CATGGACAAA AGGGTGTCTG TGGCCTGATC GTCCCCCAGG AAGACATGCC GTTCTGTGAT 
TCTGGCATCT GTCCCGACAT CATAATGAAC CCACACGGCT TCCCATCAAG AATGACGGTT 
GGAAAGTTAA TTGAGCTGCT GGCTGGCAAG GCAGGCGTGT TAGACGGCAG GTTCCACTAC 
GGCACTGCCT TTGGAGGGAG TAAAGTGAAG GATGTGTGTG AAGATCTCGT TCGCCATGGT 
TACAACTACC TGGGGAAGGA CTACGTCACC TCAGGCATCA CAGGTGAGCC CTTAGAAGCA 
TACATCTATT TTGGACCTGT GTATTATCAG AAACTGAAAC ACATGGTGCT GGATAAAATG 
CACGCCCGGG CCCGAGGACC ACGAGCTGTC CTTACCAGGC AGCCCACTGA AGGGCGGTCG 
CGCGATGGTG GCTTGCGACT TGGAGAAATG GAACGCGACT GTCTAATCGG CTATGGAGCG 
AGTATGCTTT TGCTGGAGAG ACTAATGATC TCAAGTGATG CCTTTGAGGT TGACGTCTGC 
GGGCAGTGTG GACTCCTGGG ATATTCTGGC TGGTGCCACT ACTGCAAATC TTCGTGCCAC 
GTGTCCTCCC TCCGCATCCC GTATGCCTGC AAGCTGCTCT TCCAGGAGTT ACAGTCCATG 
AACATCATCC CCAGGCTGAA ACTGGCCAAG TACAACGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The leukodystrophy mutation Polr3b R103H causes homozygote mouse embryonic lethality and impairs RNA polymerase III biogenesis.
Molecular brain12(1)59(2019 06)
Choquet K,Pinard M,Yang S,Moir RD,Poitras C,Dicaire MJ,Sgarioto N,Larivière R,Kleinman CL,Willis IM,Gauthier MS,Coulombe B,Brais B

Association of brain immune genes with social behavior of inbred mouse strains.
Journal of neuroinflammation1275(2015 Apr)
Ma L,Piirainen S,Kulesskaya N,Rauvala H,Tian L

Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and disrupts zebrafish digestive development.
PLoS biology5(11)e312(2007 Nov)
Yee NS,Gong W,Huang Y,Lorent K,Dolan AC,Maraia RJ,Pack M

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Polr3b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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