POLR3B cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR3B
Entrez Gene ID	474531
Full Name	RNA polymerase III subunit B
General protein information	Preferred Names RNA polymerase III subunit B Names DNA-directed RNA polymerase III subunit RPC2 polymerase (RNA) III (DNA directed) polypeptide B
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005625951.2	XP_005626008.1	DNA-directed RNA polymerase III subunit RPC2 isoform X3
XM_005625950.2	XP_005626007.1	DNA-directed RNA polymerase III subunit RPC2 isoform X2
XM_005625950.3	XP_005626007.1	DNA-directed RNA polymerase III subunit RPC2 isoform X2
XM_022424517.1	XP_022280225.1	DNA-directed RNA polymerase III subunit RPC2 isoform X4
XM_531760.6	XP_531760.2	DNA-directed RNA polymerase III subunit RPC2 isoform X1
XM_005625951.3	XP_005626008.1	DNA-directed RNA polymerase III subunit RPC2 isoform X3
XM_531760.5	XP_531760.2	DNA-directed RNA polymerase III subunit RPC2 isoform X1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F01078g	XP_455128.1
Schizosaccharomyces pombe (fission yeast)	rpc2	NP_593690.1
Drosophila melanogaster (fruit fly)	RpIII128	NP_523706.1
Mus musculus (house mouse)	Polr3b	NP_081699.1
Rattus norvegicus (Norway rat)	Polr3b	NP_001178807.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008151	XP_317312.4
Oryza sativa (rice)	Os03g0403100	NP_001050323.1
Neurospora crassa	NCU01772	XP_957195.1
Homo sapiens (human)	POLR3B	NP_060552.4
Gallus gallus (chicken)	POLR3B	XP_416307.3
Danio rerio (zebrafish)	polr3b	XP_690811.2
Magnaporthe oryzae (rice blast fungus)	MGG_06984	XP_003709735.1
Macaca mulatta (Rhesus monkey)	POLR3B	XP_001099515.1
Xenopus tropicalis (tropical clawed frog)	polr3b	NP_001121521.1
Pan troglodytes (chimpanzee)	POLR3B	XP_001161567.1
Canis lupus familiaris (dog)	POLR3B	XP_531760.2
Bos taurus (cattle)	POLR3B	NP_001179718.1
Caenorhabditis elegans (roundworm)	F09F7.3	NP_498192.2
Saccharomyces cerevisiae (baker's yeast)	RET1	NP_014850.1
Arabidopsis thaliana (thale cress)	NRPC2	NP_199327.4
Eremothecium gossypii	AGOS_ADL275C	NP_983821.2

Gene Ontology

The following POLR3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe291509	XM_022424517.1 Latest version!	Canis lupus familiaris RNA polymerase III subunit B (POLR3B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCe13572	XM_531760.6 Latest version!	Canis lupus familiaris RNA polymerase III subunit B (POLR3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe13573	XM_005625950.3 Latest version!	Canis lupus familiaris RNA polymerase III subunit B (POLR3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe13574	XM_005625951.3 Latest version!	Canis lupus familiaris RNA polymerase III subunit B (POLR3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCe13573	XM_005625950.2	Canis lupus familiaris polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe13574	XM_005625951.2	Canis lupus familiaris polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe13572	XM_531760.5	Canis lupus familiaris polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR3B ( XM_022424517.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022280225.1 Canis lupus familiaris RNA polymerase III subunit B (POLR3B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe291509
Clone ID Related Accession (Same CDS sequence)	XM_022424517.1
Accession Version	XM_022424517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	DNA-directed RNA polymerase III subunit RPC2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTGC TCGCGGAGGA GTTCGGGAAC CTGACCCCAG AGCAGCTGGC GGCGCCCATC 
CCCACCGTAG AGGAAAAATG GAGGCTACTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG 
AAACAGCATA TAGATTCATT CAACTATTTC ATTAATGTGG AGATAAAGAA GATCATGAAG 
GCCAACGAAA AGGTCACGAG TGATGCCGAC CCCATGTGGT ACCTGAAGTA TCTTAATATC 
TATGTGGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT ATCCCCTCAT 
GAGTGCCGTT TACGGGATAT GACGTACTCT GCCCCCATCA CCGTGGATAT TGAATATACC 
CGAGGCAGCC AGAGGATCAT CCGTAATGCC TTACCAATTG GCAGAATGCC CATAATGCTG 
CGTAGTTCGA ACTGTGTTCT TACAGGGAAG ACACCAGCAG AATTTGCCAA ACTGAACGAA 
TGTCCTTTAG ATCCAGGTGG CTACTTCATT GTCAAAGGAG TAGAAAAAGT AATTCTTATC 
CAAGAGCAGC TGTCTAAGAA CAGGATCATT GTGGAGGCTG ACAGGAAAGG GACGGTTGGT 
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAG 
GGGCGATTTT ATTTGAGACA TAATACTCTG TCAGAAGATA TTCCCATTGT CATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTACAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGTTTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG 
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGGAGGC AAAGGATGTG GGGCGGTGGA 
CCAAAGAAGA CGAAGATAGA AGAGGCAAGA GAGCTCCTGG CTTCTACCAT CCTCACACAC 
GTGCCAGTTA AAGAATTCAA TTTCCGAGCG AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGAGTAATTC TGGCCCAAGG AGATAATAAA GTGGATGACC GAGACTATTA TGGTAACAAG 
CGATTGGAGT TGGCAGGACA GCTTTTATCA CTTCTTTTTG AAGATTTATT CAAAAAATTT 
AATTCTGAAA TGAAGAAGAT TGCAGACCAG GTGATTCCTA AGCAAAGAGC AGCTCAGTTT 
GATGTTGTGA AACACATGCG TCAGGACCAG ATTACCAACG GCATGGTGAA TGCCATTTCT 
ACAGGAAATT GGTCTCTCAA GAGATTTAAA ATGGACCGCC AGGGTGTGAC TCAAGTCTTG 
TCTCGCTTGT CATATATATC TGCCCTGGGC ATGATGACAA GAATCTCCTC TCAGTTTGAA 
AAAACAAGAA AAGTGAGTGG TCCTCGTTCC CTCCAGCCAT CTCAGTGGGG AATGCTCTGT 
CCCTCGGACA CTCCTGAAGG AGAGGCATGT GGTTTGGTTA AAAACTTGGC CCTTATGACG 
CACATCACAA CTGACATGGA AGATGGACCC ATTGTTAAAT TAGCCAGTAA CCTGGGGGTA 
GAAGATGTGA ATTTATTATG TGGGGAAGAA CTCTCTTATC CAAATGTGTT TCTCGTCTTC 
CTCAATGGTA ACATCCTGGG TGTGATTCGA GACCACAAGA AGCTAGTGAA TACATTTCGA 
CTGATGCGAA GAGCAGGCTA TATCAATGAA TTTGTTTCCA TCTCAACAAA TCTCACAGAT 
CGATGTGTCT ATATTTCCTC TGATGGAGGA AGGCTTTGCA GACCCTACAT TATCGTTAAG 
AAACAGAAGC CTGCAGTCAC AAATAAGCAT ATGGAAGAGC TGGCTCAGGG ATACAGGAAT 
TTTGAAGACT TCTTACATGA GAGTCTGGTT GAATATTTAG ATGTGAATGA AGAAAATGAC 
TGTAACATCG CACTCTATGA ACATACAATT AATAAGGATA CCACCCACTT GGAGATTGAG 
CCCTTTACTC TCCTTGGTGT TTGTGCCGGC CTCATCCCAT ACCCTCATCA CAACCAGTCC 
CCAAGAAACA CATATCAGTG TGCCATGGGG AAGCAAGCCA TGGGTACCAT TGGATACAAC 
CAGCGGAACA GAATCGATAC TCTCATGTAT CTACTAGCAT ACCCACAAAA ACCCATGGTT 
AAAACAAAGA CCATTGAGTT GATAGAATTT GAGAAACTGC CAGCTGGACA AAATGCAACA 
GTTGCCGTGA TGAGTTACAG TGGCTATGAT ATTGAAGATG CCCTGGTTTT AAACAAGGCG 
TCCCTGGACA GGGGCTTTGG GCGTTGTCTT GTATATAAAA ATGCTAAATG TACACTGAAA 
CGCTATACCA ATCAGACCTT TGATAAAGTG ATGGGGCCGA TGTTGGATGC TGCTACAAGG 
AAACCCATCT GGCGACATGA AATTTTAGAT GCGGATGGTA TTTGTTCTCC AGGTGAGAAG 
GTAGAAAACA AACAAGTGCT GGTAAATAAG TCCATGCCCA CAGTAACCCA GATTCCTTTG 
GAAGGAAGTA ATGTGCCACA GCAGCCACAG TACAAAGATG TGCCCATCAC CTACAAAGGA 
GCAACGGATT CCTACATTGA AAAAGTGATG ATCTCTTCAA ATGCTGAAGA TGCTTTTCTG 
ATCAAAATGC TGCTGAGACA GACCAGGCGT CCAGAGATTG GAGACAAATT CAGCAGCCGT 
CATGGGCAAA AAGATGGCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280225.1
CDS	162..2885
Translation	MDVLAEEFGNLTPEQLAAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGTVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFRLMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKDGF

Target ORF information:

RefSeq Version	XM_022424517.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RNA polymerase III subunit B (POLR3B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424517.1

ORF Insert Sequence:

ATGGATGTGC TCGCGGAGGA GTTCGGGAAC CTGACCCCAG AGCAGCTGGC GGCGCCCATC 
CCCACCGTAG AGGAAAAATG GAGGCTACTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG 
AAACAGCATA TAGATTCATT CAACTATTTC ATTAATGTGG AGATAAAGAA GATCATGAAG 
GCCAACGAAA AGGTCACGAG TGATGCCGAC CCCATGTGGT ACCTGAAGTA TCTTAATATC 
TATGTGGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT ATCCCCTCAT 
GAGTGCCGTT TACGGGATAT GACGTACTCT GCCCCCATCA CCGTGGATAT TGAATATACC 
CGAGGCAGCC AGAGGATCAT CCGTAATGCC TTACCAATTG GCAGAATGCC CATAATGCTG 
CGTAGTTCGA ACTGTGTTCT TACAGGGAAG ACACCAGCAG AATTTGCCAA ACTGAACGAA 
TGTCCTTTAG ATCCAGGTGG CTACTTCATT GTCAAAGGAG TAGAAAAAGT AATTCTTATC 
CAAGAGCAGC TGTCTAAGAA CAGGATCATT GTGGAGGCTG ACAGGAAAGG GACGGTTGGT 
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAG 
GGGCGATTTT ATTTGAGACA TAATACTCTG TCAGAAGATA TTCCCATTGT CATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTACAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGTTTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG 
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGGAGGC AAAGGATGTG GGGCGGTGGA 
CCAAAGAAGA CGAAGATAGA AGAGGCAAGA GAGCTCCTGG CTTCTACCAT CCTCACACAC 
GTGCCAGTTA AAGAATTCAA TTTCCGAGCG AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGAGTAATTC TGGCCCAAGG AGATAATAAA GTGGATGACC GAGACTATTA TGGTAACAAG 
CGATTGGAGT TGGCAGGACA GCTTTTATCA CTTCTTTTTG AAGATTTATT CAAAAAATTT 
AATTCTGAAA TGAAGAAGAT TGCAGACCAG GTGATTCCTA AGCAAAGAGC AGCTCAGTTT 
GATGTTGTGA AACACATGCG TCAGGACCAG ATTACCAACG GCATGGTGAA TGCCATTTCT 
ACAGGAAATT GGTCTCTCAA GAGATTTAAA ATGGACCGCC AGGGTGTGAC TCAAGTCTTG 
TCTCGCTTGT CATATATATC TGCCCTGGGC ATGATGACAA GAATCTCCTC TCAGTTTGAA 
AAAACAAGAA AAGTGAGTGG TCCTCGTTCC CTCCAGCCAT CTCAGTGGGG AATGCTCTGT 
CCCTCGGACA CTCCTGAAGG AGAGGCATGT GGTTTGGTTA AAAACTTGGC CCTTATGACG 
CACATCACAA CTGACATGGA AGATGGACCC ATTGTTAAAT TAGCCAGTAA CCTGGGGGTA 
GAAGATGTGA ATTTATTATG TGGGGAAGAA CTCTCTTATC CAAATGTGTT TCTCGTCTTC 
CTCAATGGTA ACATCCTGGG TGTGATTCGA GACCACAAGA AGCTAGTGAA TACATTTCGA 
CTGATGCGAA GAGCAGGCTA TATCAATGAA TTTGTTTCCA TCTCAACAAA TCTCACAGAT 
CGATGTGTCT ATATTTCCTC TGATGGAGGA AGGCTTTGCA GACCCTACAT TATCGTTAAG 
AAACAGAAGC CTGCAGTCAC AAATAAGCAT ATGGAAGAGC TGGCTCAGGG ATACAGGAAT 
TTTGAAGACT TCTTACATGA GAGTCTGGTT GAATATTTAG ATGTGAATGA AGAAAATGAC 
TGTAACATCG CACTCTATGA ACATACAATT AATAAGGATA CCACCCACTT GGAGATTGAG 
CCCTTTACTC TCCTTGGTGT TTGTGCCGGC CTCATCCCAT ACCCTCATCA CAACCAGTCC 
CCAAGAAACA CATATCAGTG TGCCATGGGG AAGCAAGCCA TGGGTACCAT TGGATACAAC 
CAGCGGAACA GAATCGATAC TCTCATGTAT CTACTAGCAT ACCCACAAAA ACCCATGGTT 
AAAACAAAGA CCATTGAGTT GATAGAATTT GAGAAACTGC CAGCTGGACA AAATGCAACA 
GTTGCCGTGA TGAGTTACAG TGGCTATGAT ATTGAAGATG CCCTGGTTTT AAACAAGGCG 
TCCCTGGACA GGGGCTTTGG GCGTTGTCTT GTATATAAAA ATGCTAAATG TACACTGAAA 
CGCTATACCA ATCAGACCTT TGATAAAGTG ATGGGGCCGA TGTTGGATGC TGCTACAAGG 
AAACCCATCT GGCGACATGA AATTTTAGAT GCGGATGGTA TTTGTTCTCC AGGTGAGAAG 
GTAGAAAACA AACAAGTGCT GGTAAATAAG TCCATGCCCA CAGTAACCCA GATTCCTTTG 
GAAGGAAGTA ATGTGCCACA GCAGCCACAG TACAAAGATG TGCCCATCAC CTACAAAGGA 
GCAACGGATT CCTACATTGA AAAAGTGATG ATCTCTTCAA ATGCTGAAGA TGCTTTTCTG 
ATCAAAATGC TGCTGAGACA GACCAGGCGT CCAGAGATTG GAGACAAATT CAGCAGCCGT 
CATGGGCAAA AAGATGGCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR3B ( XM_531760.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_531760.2 Canis lupus familiaris RNA polymerase III subunit B (POLR3B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe13572
Clone ID Related Accession (Same CDS sequence)	XM_531760.6 , XM_531760.5
Accession Version	XM_531760.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	DNA-directed RNA polymerase III subunit RPC2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_531760.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTGC TCGCGGAGGA GTTCGGGAAC CTGACCCCAG AGCAGCTGGC GGCGCCCATC 
CCCACCGTAG AGGAAAAATG GAGGCTACTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG 
AAACAGCATA TAGATTCATT CAACTATTTC ATTAATGTGG AGATAAAGAA GATCATGAAG 
GCCAACGAAA AGGTCACGAG TGATGCCGAC CCCATGTGGT ACCTGAAGTA TCTTAATATC 
TATGTGGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT ATCCCCTCAT 
GAGTGCCGTT TACGGGATAT GACGTACTCT GCCCCCATCA CCGTGGATAT TGAATATACC 
CGAGGCAGCC AGAGGATCAT CCGTAATGCC TTACCAATTG GCAGAATGCC CATAATGCTG 
CGTAGTTCGA ACTGTGTTCT TACAGGGAAG ACACCAGCAG AATTTGCCAA ACTGAACGAA 
TGTCCTTTAG ATCCAGGTGG CTACTTCATT GTCAAAGGAG TAGAAAAAGT AATTCTTATC 
CAAGAGCAGC TGTCTAAGAA CAGGATCATT GTGGAGGCTG ACAGGAAAGG GACGGTTGGT 
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAG 
GGGCGATTTT ATTTGAGACA TAATACTCTG TCAGAAGATA TTCCCATTGT CATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTACAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGTTTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG 
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGGAGGC AAAGGATGTG GGGCGGTGGA 
CCAAAGAAGA CGAAGATAGA AGAGGCAAGA GAGCTCCTGG CTTCTACCAT CCTCACACAC 
GTGCCAGTTA AAGAATTCAA TTTCCGAGCG AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGAGTAATTC TGGCCCAAGG AGATAATAAA GTGGATGACC GAGACTATTA TGGTAACAAG 
CGATTGGAGT TGGCAGGACA GCTTTTATCA CTTCTTTTTG AAGATTTATT CAAAAAATTT 
AATTCTGAAA TGAAGAAGAT TGCAGACCAG GTGATTCCTA AGCAAAGAGC AGCTCAGTTT 
GATGTTGTGA AACACATGCG TCAGGACCAG ATTACCAACG GCATGGTGAA TGCCATTTCT 
ACAGGAAATT GGTCTCTCAA GAGATTTAAA ATGGACCGCC AGGGTGTGAC TCAAGTCTTG 
TCTCGCTTGT CATATATATC TGCCCTGGGC ATGATGACAA GAATCTCCTC TCAGTTTGAA 
AAAACAAGAA AAGTGAGTGG TCCTCGTTCC CTCCAGCCAT CTCAGTGGGG AATGCTCTGT 
CCCTCGGACA CTCCTGAAGG AGAGGCATGT GGTTTGGTTA AAAACTTGGC CCTTATGACG 
CACATCACAA CTGACATGGA AGATGGACCC ATTGTTAAAT TAGCCAGTAA CCTGGGGGTA 
GAAGATGTGA ATTTATTATG TGGGGAAGAA CTCTCTTATC CAAATGTGTT TCTCGTCTTC 
CTCAATGGTA ACATCCTGGG TGTGATTCGA GACCACAAGA AGCTAGTGAA TACATTTCGA 
CTGATGCGAA GAGCAGGCTA TATCAATGAA TTTGTTTCCA TCTCAACAAA TCTCACAGAT 
CGATGTGTCT ATATTTCCTC TGATGGAGGA AGGCTTTGCA GACCCTACAT TATCGTTAAG 
AAACAGAAGC CTGCAGTCAC AAATAAGCAT ATGGAAGAGC TGGCTCAGGG ATACAGGAAT 
TTTGAAGACT TCTTACATGA GAGTCTGGTT GAATATTTAG ATGTGAATGA AGAAAATGAC 
TGTAACATCG CACTCTATGA ACATACAATT AATAAGGATA CCACCCACTT GGAGATTGAG 
CCCTTTACTC TCCTTGGTGT TTGTGCCGGC CTCATCCCAT ACCCTCATCA CAACCAGTCC 
CCAAGAAACA CATATCAGTG TGCCATGGGG AAGCAAGCCA TGGGTACCAT TGGATACAAC 
CAGCGGAACA GAATCGATAC TCTCATGTAT CTACTAGCAT ACCCACAAAA ACCCATGGTT 
AAAACAAAGA CCATTGAGTT GATAGAATTT GAGAAACTGC CAGCTGGACA AAATGCAACA 
GTTGCCGTGA TGAGTTACAG TGGCTATGAT ATTGAAGATG CCCTGGTTTT AAACAAGGCG 
TCCCTGGACA GGGGCTTTGG GCGTTGTCTT GTATATAAAA ATGCTAAATG TACACTGAAA 
CGCTATACCA ATCAGACCTT TGATAAAGTG ATGGGGCCGA TGTTGGATGC TGCTACAAGG 
AAACCCATCT GGCGACATGA AATTTTAGAT GCGGATGGTA TTTGTTCTCC AGGTGAGAAG 
GTAGAAAACA AACAAGTGCT GGTAAATAAG TCCATGCCCA CAGTAACCCA GATTCCTTTG 
GAAGGAAGTA ATGTGCCACA GCAGCCACAG TACAAAGATG TGCCCATCAC CTACAAAGGA 
GCAACGGATT CCTACATTGA AAAAGTGATG ATCTCTTCAA ATGCTGAAGA TGCTTTTCTG 
ATCAAAATGC TGCTGAGACA GACCAGGCGT CCAGAGATTG GAGACAAATT CAGCAGCCGT 
CATGGGCAAA AAGGTGTTTG CGGCTTGATC GTCCCGCAGG AAGACATGCC ATTCTGTGAT 
TCTGGCATCT GTCCGGACAT CATAATGAAC CCACACGGCT TCCCATCACG AATGACGGTG 
GGAAAGCTGA TTGAGCTGTT GGCGGGCAAG GCTGGCGTGC TGGACGGGAG GTTCCACTAT 
GGCACTGCGT TTGGAGGCAG CAAAGTGAAA GATGTGTGTG AAGACCTCGT TCGCCATGGT 
TATAACTACT TGGGGAAAGA CTTTGTGACA TCTGGCATTA CAGGCGAGCC CTTAGAAGCA 
TACATCTATT TTGGCCCGGT GTACTATCAG AAACTGAAGC ACATGGTGTT GGATAAGATG 
CATGCCCGAG CCCGGGGGCC ACGAGCTGTT CTCACCAGGC AGCCCACTGA AGGTCGGTCT 
CGTGATGGCG GTTTGCGTCT TGGAGAAATG GAACGTGACT GTTTAATCGG CTACGGAGCC 
AGCATGCTCC TACTAGAGAG ACTGATGATT TCAAGTGATG CCTTTGAAGT TGATGTCTGT 
GGGCAGTGTG GACTTCTGGG GTATTCTGGC TGGTGCCATT ACTGCAAGTC ATCATGCCAT 
GTGTCTTCCC TCCGCATCCC ATATGCCTGC AAGCTGCTCT TCCAGGAGCT GCAGTCTATG 
AACATCATCC CCCGGTTAAA ACTGTCCAAA TACAATGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_531760.2
CDS	162..3563
Translation	MDVLAEEFGNLTPEQLAAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGTVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFRLMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHGYNYLGKDFVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLSKYNE

Target ORF information:

RefSeq Version	XM_531760.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RNA polymerase III subunit B (POLR3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_531760.6

ORF Insert Sequence:

ATGGATGTGC TCGCGGAGGA GTTCGGGAAC CTGACCCCAG AGCAGCTGGC GGCGCCCATC 
CCCACCGTAG AGGAAAAATG GAGGCTACTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG 
AAACAGCATA TAGATTCATT CAACTATTTC ATTAATGTGG AGATAAAGAA GATCATGAAG 
GCCAACGAAA AGGTCACGAG TGATGCCGAC CCCATGTGGT ACCTGAAGTA TCTTAATATC 
TATGTGGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT ATCCCCTCAT 
GAGTGCCGTT TACGGGATAT GACGTACTCT GCCCCCATCA CCGTGGATAT TGAATATACC 
CGAGGCAGCC AGAGGATCAT CCGTAATGCC TTACCAATTG GCAGAATGCC CATAATGCTG 
CGTAGTTCGA ACTGTGTTCT TACAGGGAAG ACACCAGCAG AATTTGCCAA ACTGAACGAA 
TGTCCTTTAG ATCCAGGTGG CTACTTCATT GTCAAAGGAG TAGAAAAAGT AATTCTTATC 
CAAGAGCAGC TGTCTAAGAA CAGGATCATT GTGGAGGCTG ACAGGAAAGG GACGGTTGGT 
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAG 
GGGCGATTTT ATTTGAGACA TAATACTCTG TCAGAAGATA TTCCCATTGT CATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTACAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGTTTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG 
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGGAGGC AAAGGATGTG GGGCGGTGGA 
CCAAAGAAGA CGAAGATAGA AGAGGCAAGA GAGCTCCTGG CTTCTACCAT CCTCACACAC 
GTGCCAGTTA AAGAATTCAA TTTCCGAGCG AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGAGTAATTC TGGCCCAAGG AGATAATAAA GTGGATGACC GAGACTATTA TGGTAACAAG 
CGATTGGAGT TGGCAGGACA GCTTTTATCA CTTCTTTTTG AAGATTTATT CAAAAAATTT 
AATTCTGAAA TGAAGAAGAT TGCAGACCAG GTGATTCCTA AGCAAAGAGC AGCTCAGTTT 
GATGTTGTGA AACACATGCG TCAGGACCAG ATTACCAACG GCATGGTGAA TGCCATTTCT 
ACAGGAAATT GGTCTCTCAA GAGATTTAAA ATGGACCGCC AGGGTGTGAC TCAAGTCTTG 
TCTCGCTTGT CATATATATC TGCCCTGGGC ATGATGACAA GAATCTCCTC TCAGTTTGAA 
AAAACAAGAA AAGTGAGTGG TCCTCGTTCC CTCCAGCCAT CTCAGTGGGG AATGCTCTGT 
CCCTCGGACA CTCCTGAAGG AGAGGCATGT GGTTTGGTTA AAAACTTGGC CCTTATGACG 
CACATCACAA CTGACATGGA AGATGGACCC ATTGTTAAAT TAGCCAGTAA CCTGGGGGTA 
GAAGATGTGA ATTTATTATG TGGGGAAGAA CTCTCTTATC CAAATGTGTT TCTCGTCTTC 
CTCAATGGTA ACATCCTGGG TGTGATTCGA GACCACAAGA AGCTAGTGAA TACATTTCGA 
CTGATGCGAA GAGCAGGCTA TATCAATGAA TTTGTTTCCA TCTCAACAAA TCTCACAGAT 
CGATGTGTCT ATATTTCCTC TGATGGAGGA AGGCTTTGCA GACCCTACAT TATCGTTAAG 
AAACAGAAGC CTGCAGTCAC AAATAAGCAT ATGGAAGAGC TGGCTCAGGG ATACAGGAAT 
TTTGAAGACT TCTTACATGA GAGTCTGGTT GAATATTTAG ATGTGAATGA AGAAAATGAC 
TGTAACATCG CACTCTATGA ACATACAATT AATAAGGATA CCACCCACTT GGAGATTGAG 
CCCTTTACTC TCCTTGGTGT TTGTGCCGGC CTCATCCCAT ACCCTCATCA CAACCAGTCC 
CCAAGAAACA CATATCAGTG TGCCATGGGG AAGCAAGCCA TGGGTACCAT TGGATACAAC 
CAGCGGAACA GAATCGATAC TCTCATGTAT CTACTAGCAT ACCCACAAAA ACCCATGGTT 
AAAACAAAGA CCATTGAGTT GATAGAATTT GAGAAACTGC CAGCTGGACA AAATGCAACA 
GTTGCCGTGA TGAGTTACAG TGGCTATGAT ATTGAAGATG CCCTGGTTTT AAACAAGGCG 
TCCCTGGACA GGGGCTTTGG GCGTTGTCTT GTATATAAAA ATGCTAAATG TACACTGAAA 
CGCTATACCA ATCAGACCTT TGATAAAGTG ATGGGGCCGA TGTTGGATGC TGCTACAAGG 
AAACCCATCT GGCGACATGA AATTTTAGAT GCGGATGGTA TTTGTTCTCC AGGTGAGAAG 
GTAGAAAACA AACAAGTGCT GGTAAATAAG TCCATGCCCA CAGTAACCCA GATTCCTTTG 
GAAGGAAGTA ATGTGCCACA GCAGCCACAG TACAAAGATG TGCCCATCAC CTACAAAGGA 
GCAACGGATT CCTACATTGA AAAAGTGATG ATCTCTTCAA ATGCTGAAGA TGCTTTTCTG 
ATCAAAATGC TGCTGAGACA GACCAGGCGT CCAGAGATTG GAGACAAATT CAGCAGCCGT 
CATGGGCAAA AAGGTGTTTG CGGCTTGATC GTCCCGCAGG AAGACATGCC ATTCTGTGAT 
TCTGGCATCT GTCCGGACAT CATAATGAAC CCACACGGCT TCCCATCACG AATGACGGTG 
GGAAAGCTGA TTGAGCTGTT GGCGGGCAAG GCTGGCGTGC TGGACGGGAG GTTCCACTAT 
GGCACTGCGT TTGGAGGCAG CAAAGTGAAA GATGTGTGTG AAGACCTCGT TCGCCATGGT 
TATAACTACT TGGGGAAAGA CTTTGTGACA TCTGGCATTA CAGGCGAGCC CTTAGAAGCA 
TACATCTATT TTGGCCCGGT GTACTATCAG AAACTGAAGC ACATGGTGTT GGATAAGATG 
CATGCCCGAG CCCGGGGGCC ACGAGCTGTT CTCACCAGGC AGCCCACTGA AGGTCGGTCT 
CGTGATGGCG GTTTGCGTCT TGGAGAAATG GAACGTGACT GTTTAATCGG CTACGGAGCC 
AGCATGCTCC TACTAGAGAG ACTGATGATT TCAAGTGATG CCTTTGAAGT TGATGTCTGT 
GGGCAGTGTG GACTTCTGGG GTATTCTGGC TGGTGCCATT ACTGCAAGTC ATCATGCCAT 
GTGTCTTCCC TCCGCATCCC ATATGCCTGC AAGCTGCTCT TCCAGGAGCT GCAGTCTATG 
AACATCATCC CCCGGTTAAA ACTGTCCAAA TACAATGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR3B ( XM_531760.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_531760.2 Canis lupus familiaris polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe13572
Clone ID Related Accession (Same CDS sequence)	XM_531760.6 , XM_531760.5
Accession Version	XM_531760.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	DNA-directed RNA polymerase III subunit RPC2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_531760.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTGC TCGCGGAGGA GTTCGGGAAC CTGACCCCAG AGCAGCTGGC GGCGCCCATC 
CCCACCGTAG AGGAAAAATG GAGGCTACTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG 
AAACAGCATA TAGATTCATT CAACTATTTC ATTAATGTGG AGATAAAGAA GATCATGAAG 
GCCAACGAAA AGGTCACGAG TGATGCCGAC CCCATGTGGT ACCTGAAGTA TCTTAATATC 
TATGTGGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT ATCCCCTCAT 
GAGTGCCGTT TACGGGATAT GACGTACTCT GCCCCCATCA CCGTGGATAT TGAATATACC 
CGAGGCAGCC AGAGGATCAT CCGTAATGCC TTACCAATTG GCAGAATGCC CATAATGCTG 
CGTAGTTCGA ACTGTGTTCT TACAGGGAAG ACACCAGCAG AATTTGCCAA ACTGAACGAA 
TGTCCTTTAG ATCCAGGTGG CTACTTCATT GTCAAAGGAG TAGAAAAAGT AATTCTTATC 
CAAGAGCAGC TGTCTAAGAA CAGGATCATT GTGGAGGCTG ACAGGAAAGG GACGGTTGGT 
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAG 
GGGCGATTTT ATTTGAGACA TAATACTCTG TCAGAAGATA TTCCCATTGT CATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTACAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGTTTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG 
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGGAGGC AAAGGATGTG GGGCGGTGGA 
CCAAAGAAGA CGAAGATAGA AGAGGCAAGA GAGCTCCTGG CTTCTACCAT CCTCACACAC 
GTGCCAGTTA AAGAATTCAA TTTCCGAGCG AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGAGTAATTC TGGCCCAAGG AGATAATAAA GTGGATGACC GAGACTATTA TGGTAACAAG 
CGATTGGAGT TGGCAGGACA GCTTTTATCA CTTCTTTTTG AAGATTTATT CAAAAAATTT 
AATTCTGAAA TGAAGAAGAT TGCAGACCAG GTGATTCCTA AGCAAAGAGC AGCTCAGTTT 
GATGTTGTGA AACACATGCG TCAGGACCAG ATTACCAACG GCATGGTGAA TGCCATTTCT 
ACAGGAAATT GGTCTCTCAA GAGATTTAAA ATGGACCGCC AGGGTGTGAC TCAAGTCTTG 
TCTCGCTTGT CATATATATC TGCCCTGGGC ATGATGACAA GAATCTCCTC TCAGTTTGAA 
AAAACAAGAA AAGTGAGTGG TCCTCGTTCC CTCCAGCCAT CTCAGTGGGG AATGCTCTGT 
CCCTCGGACA CTCCTGAAGG AGAGGCATGT GGTTTGGTTA AAAACTTGGC CCTTATGACG 
CACATCACAA CTGACATGGA AGATGGACCC ATTGTTAAAT TAGCCAGTAA CCTGGGGGTA 
GAAGATGTGA ATTTATTATG TGGGGAAGAA CTCTCTTATC CAAATGTGTT TCTCGTCTTC 
CTCAATGGTA ACATCCTGGG TGTGATTCGA GACCACAAGA AGCTAGTGAA TACATTTCGA 
CTGATGCGAA GAGCAGGCTA TATCAATGAA TTTGTTTCCA TCTCAACAAA TCTCACAGAT 
CGATGTGTCT ATATTTCCTC TGATGGAGGA AGGCTTTGCA GACCCTACAT TATCGTTAAG 
AAACAGAAGC CTGCAGTCAC AAATAAGCAT ATGGAAGAGC TGGCTCAGGG ATACAGGAAT 
TTTGAAGACT TCTTACATGA GAGTCTGGTT GAATATTTAG ATGTGAATGA AGAAAATGAC 
TGTAACATCG CACTCTATGA ACATACAATT AATAAGGATA CCACCCACTT GGAGATTGAG 
CCCTTTACTC TCCTTGGTGT TTGTGCCGGC CTCATCCCAT ACCCTCATCA CAACCAGTCC 
CCAAGAAACA CATATCAGTG TGCCATGGGG AAGCAAGCCA TGGGTACCAT TGGATACAAC 
CAGCGGAACA GAATCGATAC TCTCATGTAT CTACTAGCAT ACCCACAAAA ACCCATGGTT 
AAAACAAAGA CCATTGAGTT GATAGAATTT GAGAAACTGC CAGCTGGACA AAATGCAACA 
GTTGCCGTGA TGAGTTACAG TGGCTATGAT ATTGAAGATG CCCTGGTTTT AAACAAGGCG 
TCCCTGGACA GGGGCTTTGG GCGTTGTCTT GTATATAAAA ATGCTAAATG TACACTGAAA 
CGCTATACCA ATCAGACCTT TGATAAAGTG ATGGGGCCGA TGTTGGATGC TGCTACAAGG 
AAACCCATCT GGCGACATGA AATTTTAGAT GCGGATGGTA TTTGTTCTCC AGGTGAGAAG 
GTAGAAAACA AACAAGTGCT GGTAAATAAG TCCATGCCCA CAGTAACCCA GATTCCTTTG 
GAAGGAAGTA ATGTGCCACA GCAGCCACAG TACAAAGATG TGCCCATCAC CTACAAAGGA 
GCAACGGATT CCTACATTGA AAAAGTGATG ATCTCTTCAA ATGCTGAAGA TGCTTTTCTG 
ATCAAAATGC TGCTGAGACA GACCAGGCGT CCAGAGATTG GAGACAAATT CAGCAGCCGT 
CATGGGCAAA AAGGTGTTTG CGGCTTGATC GTCCCGCAGG AAGACATGCC ATTCTGTGAT 
TCTGGCATCT GTCCGGACAT CATAATGAAC CCACACGGCT TCCCATCACG AATGACGGTG 
GGAAAGCTGA TTGAGCTGTT GGCGGGCAAG GCTGGCGTGC TGGACGGGAG GTTCCACTAT 
GGCACTGCGT TTGGAGGCAG CAAAGTGAAA GATGTGTGTG AAGACCTCGT TCGCCATGGT 
TATAACTACT TGGGGAAAGA CTTTGTGACA TCTGGCATTA CAGGCGAGCC CTTAGAAGCA 
TACATCTATT TTGGCCCGGT GTACTATCAG AAACTGAAGC ACATGGTGTT GGATAAGATG 
CATGCCCGAG CCCGGGGGCC ACGAGCTGTT CTCACCAGGC AGCCCACTGA AGGTCGGTCT 
CGTGATGGCG GTTTGCGTCT TGGAGAAATG GAACGTGACT GTTTAATCGG CTACGGAGCC 
AGCATGCTCC TACTAGAGAG ACTGATGATT TCAAGTGATG CCTTTGAAGT TGATGTCTGT 
GGGCAGTGTG GACTTCTGGG GTATTCTGGC TGGTGCCATT ACTGCAAGTC ATCATGCCAT 
GTGTCTTCCC TCCGCATCCC ATATGCCTGC AAGCTGCTCT TCCAGGAGCT GCAGTCTATG 
AACATCATCC CCCGGTTAAA ACTGTCCAAA TACAATGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_531760.2
CDS	1963..5364
Translation	MDVLAEEFGNLTPEQLAAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGTVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFRLMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHGYNYLGKDFVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLSKYNE

Target ORF information:

RefSeq Version	XM_531760.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_531760.5

ORF Insert Sequence:

ATGGATGTGC TCGCGGAGGA GTTCGGGAAC CTGACCCCAG AGCAGCTGGC GGCGCCCATC 
CCCACCGTAG AGGAAAAATG GAGGCTACTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG 
AAACAGCATA TAGATTCATT CAACTATTTC ATTAATGTGG AGATAAAGAA GATCATGAAG 
GCCAACGAAA AGGTCACGAG TGATGCCGAC CCCATGTGGT ACCTGAAGTA TCTTAATATC 
TATGTGGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT ATCCCCTCAT 
GAGTGCCGTT TACGGGATAT GACGTACTCT GCCCCCATCA CCGTGGATAT TGAATATACC 
CGAGGCAGCC AGAGGATCAT CCGTAATGCC TTACCAATTG GCAGAATGCC CATAATGCTG 
CGTAGTTCGA ACTGTGTTCT TACAGGGAAG ACACCAGCAG AATTTGCCAA ACTGAACGAA 
TGTCCTTTAG ATCCAGGTGG CTACTTCATT GTCAAAGGAG TAGAAAAAGT AATTCTTATC 
CAAGAGCAGC TGTCTAAGAA CAGGATCATT GTGGAGGCTG ACAGGAAAGG GACGGTTGGT 
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAG 
GGGCGATTTT ATTTGAGACA TAATACTCTG TCAGAAGATA TTCCCATTGT CATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTACAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGTTTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG 
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGGAGGC AAAGGATGTG GGGCGGTGGA 
CCAAAGAAGA CGAAGATAGA AGAGGCAAGA GAGCTCCTGG CTTCTACCAT CCTCACACAC 
GTGCCAGTTA AAGAATTCAA TTTCCGAGCG AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGAGTAATTC TGGCCCAAGG AGATAATAAA GTGGATGACC GAGACTATTA TGGTAACAAG 
CGATTGGAGT TGGCAGGACA GCTTTTATCA CTTCTTTTTG AAGATTTATT CAAAAAATTT 
AATTCTGAAA TGAAGAAGAT TGCAGACCAG GTGATTCCTA AGCAAAGAGC AGCTCAGTTT 
GATGTTGTGA AACACATGCG TCAGGACCAG ATTACCAACG GCATGGTGAA TGCCATTTCT 
ACAGGAAATT GGTCTCTCAA GAGATTTAAA ATGGACCGCC AGGGTGTGAC TCAAGTCTTG 
TCTCGCTTGT CATATATATC TGCCCTGGGC ATGATGACAA GAATCTCCTC TCAGTTTGAA 
AAAACAAGAA AAGTGAGTGG TCCTCGTTCC CTCCAGCCAT CTCAGTGGGG AATGCTCTGT 
CCCTCGGACA CTCCTGAAGG AGAGGCATGT GGTTTGGTTA AAAACTTGGC CCTTATGACG 
CACATCACAA CTGACATGGA AGATGGACCC ATTGTTAAAT TAGCCAGTAA CCTGGGGGTA 
GAAGATGTGA ATTTATTATG TGGGGAAGAA CTCTCTTATC CAAATGTGTT TCTCGTCTTC 
CTCAATGGTA ACATCCTGGG TGTGATTCGA GACCACAAGA AGCTAGTGAA TACATTTCGA 
CTGATGCGAA GAGCAGGCTA TATCAATGAA TTTGTTTCCA TCTCAACAAA TCTCACAGAT 
CGATGTGTCT ATATTTCCTC TGATGGAGGA AGGCTTTGCA GACCCTACAT TATCGTTAAG 
AAACAGAAGC CTGCAGTCAC AAATAAGCAT ATGGAAGAGC TGGCTCAGGG ATACAGGAAT 
TTTGAAGACT TCTTACATGA GAGTCTGGTT GAATATTTAG ATGTGAATGA AGAAAATGAC 
TGTAACATCG CACTCTATGA ACATACAATT AATAAGGATA CCACCCACTT GGAGATTGAG 
CCCTTTACTC TCCTTGGTGT TTGTGCCGGC CTCATCCCAT ACCCTCATCA CAACCAGTCC 
CCAAGAAACA CATATCAGTG TGCCATGGGG AAGCAAGCCA TGGGTACCAT TGGATACAAC 
CAGCGGAACA GAATCGATAC TCTCATGTAT CTACTAGCAT ACCCACAAAA ACCCATGGTT 
AAAACAAAGA CCATTGAGTT GATAGAATTT GAGAAACTGC CAGCTGGACA AAATGCAACA 
GTTGCCGTGA TGAGTTACAG TGGCTATGAT ATTGAAGATG CCCTGGTTTT AAACAAGGCG 
TCCCTGGACA GGGGCTTTGG GCGTTGTCTT GTATATAAAA ATGCTAAATG TACACTGAAA 
CGCTATACCA ATCAGACCTT TGATAAAGTG ATGGGGCCGA TGTTGGATGC TGCTACAAGG 
AAACCCATCT GGCGACATGA AATTTTAGAT GCGGATGGTA TTTGTTCTCC AGGTGAGAAG 
GTAGAAAACA AACAAGTGCT GGTAAATAAG TCCATGCCCA CAGTAACCCA GATTCCTTTG 
GAAGGAAGTA ATGTGCCACA GCAGCCACAG TACAAAGATG TGCCCATCAC CTACAAAGGA 
GCAACGGATT CCTACATTGA AAAAGTGATG ATCTCTTCAA ATGCTGAAGA TGCTTTTCTG 
ATCAAAATGC TGCTGAGACA GACCAGGCGT CCAGAGATTG GAGACAAATT CAGCAGCCGT 
CATGGGCAAA AAGGTGTTTG CGGCTTGATC GTCCCGCAGG AAGACATGCC ATTCTGTGAT 
TCTGGCATCT GTCCGGACAT CATAATGAAC CCACACGGCT TCCCATCACG AATGACGGTG 
GGAAAGCTGA TTGAGCTGTT GGCGGGCAAG GCTGGCGTGC TGGACGGGAG GTTCCACTAT 
GGCACTGCGT TTGGAGGCAG CAAAGTGAAA GATGTGTGTG AAGACCTCGT TCGCCATGGT 
TATAACTACT TGGGGAAAGA CTTTGTGACA TCTGGCATTA CAGGCGAGCC CTTAGAAGCA 
TACATCTATT TTGGCCCGGT GTACTATCAG AAACTGAAGC ACATGGTGTT GGATAAGATG 
CATGCCCGAG CCCGGGGGCC ACGAGCTGTT CTCACCAGGC AGCCCACTGA AGGTCGGTCT 
CGTGATGGCG GTTTGCGTCT TGGAGAAATG GAACGTGACT GTTTAATCGG CTACGGAGCC 
AGCATGCTCC TACTAGAGAG ACTGATGATT TCAAGTGATG CCTTTGAAGT TGATGTCTGT 
GGGCAGTGTG GACTTCTGGG GTATTCTGGC TGGTGCCATT ACTGCAAGTC ATCATGCCAT 
GTGTCTTCCC TCCGCATCCC ATATGCCTGC AAGCTGCTCT TCCAGGAGCT GCAGTCTATG 
AACATCATCC CCCGGTTAAA ACTGTCCAAA TACAATGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR3B ( XM_005625950.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005626007.1 Canis lupus familiaris polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe13573
Clone ID Related Accession (Same CDS sequence)	XM_005625950.2 , XM_005625950.3
Accession Version	XM_005625950.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	DNA-directed RNA polymerase III subunit RPC2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTGC TCGCGGAGGA GTTCGGGAAC CTGACCCCAG AGCAGCTGGC GGCGCCCATC 
CCCACCGTAG AGGAAAAATG GAGGCTACTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG 
AAACAGCATA TAGATTCATT CAACTATTTC ATTAATGTGG AGATAAAGAA GATCATGAAG 
GCCAACGAAA AGGTCACGAG TGATGCCGAC CCCATGTGGT ACCTGAAGTA TCTTAATATC 
TATGTGGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT ATCCCCTCAT 
GAGTGCCGTT TACGGGATAT GACGTACTCT GCCCCCATCA CCGTGGATAT TGAATATACC 
CGAGGCAGCC AGAGGATCAT CCGTAATGCC TTACCAATTG GCAGAATGCC CATAATGCTG 
CGTAGTTCGA ACTGTGTTCT TACAGGGAAG ACACCAGCAG AATTTGCCAA ACTGAACGAA 
TGTCCTTTAG ATCCAGGTGG CTACTTCATT GTCAAAGGAG TAGAAAAAGT AATTCTTATC 
CAAGAGCAGC TGTCTAAGAA CAGGATCATT GTGGAGGCTG ACAGGAAAGG GACGGTTGGT 
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAG 
GGGCGATTTT ATTTGAGACA TAATACTCTG TCAGAAGATA TTCCCATTGT CATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTACAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGTTTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG 
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGGAGGC AAAGGATGTG GGGCGGTGGA 
CCAAAGAAGA CGAAGATAGA AGAGGCAAGA GAGCTCCTGG CTTCTACCAT CCTCACACAC 
GTGCCAGTTA AAGAATTCAA TTTCCGAGCG AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGAGTAATTC TGGCCCAAGG AGATAATAAA GTGGATGACC GAGACTATTA TGGTAACAAG 
CGATTGGAGT TGGCAGGACA GGGAAATTGG TCTCTCAAGA GATTTAAAAT GGACCGCCAG 
GGTGTGACTC AAGTCTTGTC TCGCTTGTCA TATATATCTG CCCTGGGCAT GATGACAAGA 
ATCTCCTCTC AGTTTGAAAA AACAAGAAAA GTGAGTGGTC CTCGTTCCCT CCAGCCATCT 
CAGTGGGGAA TGCTCTGTCC CTCGGACACT CCTGAAGGAG AGGCATGTGG TTTGGTTAAA 
AACTTGGCCC TTATGACGCA CATCACAACT GACATGGAAG ATGGACCCAT TGTTAAATTA 
GCCAGTAACC TGGGGGTAGA AGATGTGAAT TTATTATGTG GGGAAGAACT CTCTTATCCA 
AATGTGTTTC TCGTCTTCCT CAATGGTAAC ATCCTGGGTG TGATTCGAGA CCACAAGAAG 
CTAGTGAATA CATTTCGACT GATGCGAAGA GCAGGCTATA TCAATGAATT TGTTTCCATC 
TCAACAAATC TCACAGATCG ATGTGTCTAT ATTTCCTCTG ATGGAGGAAG GCTTTGCAGA 
CCCTACATTA TCGTTAAGAA ACAGAAGCCT GCAGTCACAA ATAAGCATAT GGAAGAGCTG 
GCTCAGGGAT ACAGGAATTT TGAAGACTTC TTACATGAGA GTCTGGTTGA ATATTTAGAT 
GTGAATGAAG AAAATGACTG TAACATCGCA CTCTATGAAC ATACAATTAA TAAGGATACC 
ACCCACTTGG AGATTGAGCC CTTTACTCTC CTTGGTGTTT GTGCCGGCCT CATCCCATAC 
CCTCATCACA ACCAGTCCCC AAGAAACACA TATCAGTGTG CCATGGGGAA GCAAGCCATG 
GGTACCATTG GATACAACCA GCGGAACAGA ATCGATACTC TCATGTATCT ACTAGCATAC 
CCACAAAAAC CCATGGTTAA AACAAAGACC ATTGAGTTGA TAGAATTTGA GAAACTGCCA 
GCTGGACAAA ATGCAACAGT TGCCGTGATG AGTTACAGTG GCTATGATAT TGAAGATGCC 
CTGGTTTTAA ACAAGGCGTC CCTGGACAGG GGCTTTGGGC GTTGTCTTGT ATATAAAAAT 
GCTAAATGTA CACTGAAACG CTATACCAAT CAGACCTTTG ATAAAGTGAT GGGGCCGATG 
TTGGATGCTG CTACAAGGAA ACCCATCTGG CGACATGAAA TTTTAGATGC GGATGGTATT 
TGTTCTCCAG GTGAGAAGGT AGAAAACAAA CAAGTGCTGG TAAATAAGTC CATGCCCACA 
GTAACCCAGA TTCCTTTGGA AGGAAGTAAT GTGCCACAGC AGCCACAGTA CAAAGATGTG 
CCCATCACCT ACAAAGGAGC AACGGATTCC TACATTGAAA AAGTGATGAT CTCTTCAAAT 
GCTGAAGATG CTTTTCTGAT CAAAATGCTG CTGAGACAGA CCAGGCGTCC AGAGATTGGA 
GACAAATTCA GCAGCCGTCA TGGGCAAAAA GGTGTTTGCG GCTTGATCGT CCCGCAGGAA 
GACATGCCAT TCTGTGATTC TGGCATCTGT CCGGACATCA TAATGAACCC ACACGGCTTC 
CCATCACGAA TGACGGTGGG AAAGCTGATT GAGCTGTTGG CGGGCAAGGC TGGCGTGCTG 
GACGGGAGGT TCCACTATGG CACTGCGTTT GGAGGCAGCA AAGTGAAAGA TGTGTGTGAA 
GACCTCGTTC GCCATGGTTA TAACTACTTG GGGAAAGACT TTGTGACATC TGGCATTACA 
GGCGAGCCCT TAGAAGCATA CATCTATTTT GGCCCGGTGT ACTATCAGAA ACTGAAGCAC 
ATGGTGTTGG ATAAGATGCA TGCCCGAGCC CGGGGGCCAC GAGCTGTTCT CACCAGGCAG 
CCCACTGAAG GTCGGTCTCG TGATGGCGGT TTGCGTCTTG GAGAAATGGA ACGTGACTGT 
TTAATCGGCT ACGGAGCCAG CATGCTCCTA CTAGAGAGAC TGATGATTTC AAGTGATGCC 
TTTGAAGTTG ATGTCTGTGG GCAGTGTGGA CTTCTGGGGT ATTCTGGCTG GTGCCATTAC 
TGCAAGTCAT CATGCCATGT GTCTTCCCTC CGCATCCCAT ATGCCTGCAA GCTGCTCTTC 
CAGGAGCTGC AGTCTATGAA CATCATCCCC CGGTTAAAAC TGTCCAAATA CAATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626007.1
CDS	1963..5202
Translation	MDVLAEEFGNLTPEQLAAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGTVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFRLMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHGYNYLGKDFVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLSKYNE

Target ORF information:

RefSeq Version	XM_005625950.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625950.2

ORF Insert Sequence:

ATGGATGTGC TCGCGGAGGA GTTCGGGAAC CTGACCCCAG AGCAGCTGGC GGCGCCCATC 
CCCACCGTAG AGGAAAAATG GAGGCTACTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG 
AAACAGCATA TAGATTCATT CAACTATTTC ATTAATGTGG AGATAAAGAA GATCATGAAG 
GCCAACGAAA AGGTCACGAG TGATGCCGAC CCCATGTGGT ACCTGAAGTA TCTTAATATC 
TATGTGGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT ATCCCCTCAT 
GAGTGCCGTT TACGGGATAT GACGTACTCT GCCCCCATCA CCGTGGATAT TGAATATACC 
CGAGGCAGCC AGAGGATCAT CCGTAATGCC TTACCAATTG GCAGAATGCC CATAATGCTG 
CGTAGTTCGA ACTGTGTTCT TACAGGGAAG ACACCAGCAG AATTTGCCAA ACTGAACGAA 
TGTCCTTTAG ATCCAGGTGG CTACTTCATT GTCAAAGGAG TAGAAAAAGT AATTCTTATC 
CAAGAGCAGC TGTCTAAGAA CAGGATCATT GTGGAGGCTG ACAGGAAAGG GACGGTTGGT 
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAG 
GGGCGATTTT ATTTGAGACA TAATACTCTG TCAGAAGATA TTCCCATTGT CATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTACAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGTTTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG 
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGGAGGC AAAGGATGTG GGGCGGTGGA 
CCAAAGAAGA CGAAGATAGA AGAGGCAAGA GAGCTCCTGG CTTCTACCAT CCTCACACAC 
GTGCCAGTTA AAGAATTCAA TTTCCGAGCG AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGAGTAATTC TGGCCCAAGG AGATAATAAA GTGGATGACC GAGACTATTA TGGTAACAAG 
CGATTGGAGT TGGCAGGACA GGGAAATTGG TCTCTCAAGA GATTTAAAAT GGACCGCCAG 
GGTGTGACTC AAGTCTTGTC TCGCTTGTCA TATATATCTG CCCTGGGCAT GATGACAAGA 
ATCTCCTCTC AGTTTGAAAA AACAAGAAAA GTGAGTGGTC CTCGTTCCCT CCAGCCATCT 
CAGTGGGGAA TGCTCTGTCC CTCGGACACT CCTGAAGGAG AGGCATGTGG TTTGGTTAAA 
AACTTGGCCC TTATGACGCA CATCACAACT GACATGGAAG ATGGACCCAT TGTTAAATTA 
GCCAGTAACC TGGGGGTAGA AGATGTGAAT TTATTATGTG GGGAAGAACT CTCTTATCCA 
AATGTGTTTC TCGTCTTCCT CAATGGTAAC ATCCTGGGTG TGATTCGAGA CCACAAGAAG 
CTAGTGAATA CATTTCGACT GATGCGAAGA GCAGGCTATA TCAATGAATT TGTTTCCATC 
TCAACAAATC TCACAGATCG ATGTGTCTAT ATTTCCTCTG ATGGAGGAAG GCTTTGCAGA 
CCCTACATTA TCGTTAAGAA ACAGAAGCCT GCAGTCACAA ATAAGCATAT GGAAGAGCTG 
GCTCAGGGAT ACAGGAATTT TGAAGACTTC TTACATGAGA GTCTGGTTGA ATATTTAGAT 
GTGAATGAAG AAAATGACTG TAACATCGCA CTCTATGAAC ATACAATTAA TAAGGATACC 
ACCCACTTGG AGATTGAGCC CTTTACTCTC CTTGGTGTTT GTGCCGGCCT CATCCCATAC 
CCTCATCACA ACCAGTCCCC AAGAAACACA TATCAGTGTG CCATGGGGAA GCAAGCCATG 
GGTACCATTG GATACAACCA GCGGAACAGA ATCGATACTC TCATGTATCT ACTAGCATAC 
CCACAAAAAC CCATGGTTAA AACAAAGACC ATTGAGTTGA TAGAATTTGA GAAACTGCCA 
GCTGGACAAA ATGCAACAGT TGCCGTGATG AGTTACAGTG GCTATGATAT TGAAGATGCC 
CTGGTTTTAA ACAAGGCGTC CCTGGACAGG GGCTTTGGGC GTTGTCTTGT ATATAAAAAT 
GCTAAATGTA CACTGAAACG CTATACCAAT CAGACCTTTG ATAAAGTGAT GGGGCCGATG 
TTGGATGCTG CTACAAGGAA ACCCATCTGG CGACATGAAA TTTTAGATGC GGATGGTATT 
TGTTCTCCAG GTGAGAAGGT AGAAAACAAA CAAGTGCTGG TAAATAAGTC CATGCCCACA 
GTAACCCAGA TTCCTTTGGA AGGAAGTAAT GTGCCACAGC AGCCACAGTA CAAAGATGTG 
CCCATCACCT ACAAAGGAGC AACGGATTCC TACATTGAAA AAGTGATGAT CTCTTCAAAT 
GCTGAAGATG CTTTTCTGAT CAAAATGCTG CTGAGACAGA CCAGGCGTCC AGAGATTGGA 
GACAAATTCA GCAGCCGTCA TGGGCAAAAA GGTGTTTGCG GCTTGATCGT CCCGCAGGAA 
GACATGCCAT TCTGTGATTC TGGCATCTGT CCGGACATCA TAATGAACCC ACACGGCTTC 
CCATCACGAA TGACGGTGGG AAAGCTGATT GAGCTGTTGG CGGGCAAGGC TGGCGTGCTG 
GACGGGAGGT TCCACTATGG CACTGCGTTT GGAGGCAGCA AAGTGAAAGA TGTGTGTGAA 
GACCTCGTTC GCCATGGTTA TAACTACTTG GGGAAAGACT TTGTGACATC TGGCATTACA 
GGCGAGCCCT TAGAAGCATA CATCTATTTT GGCCCGGTGT ACTATCAGAA ACTGAAGCAC 
ATGGTGTTGG ATAAGATGCA TGCCCGAGCC CGGGGGCCAC GAGCTGTTCT CACCAGGCAG 
CCCACTGAAG GTCGGTCTCG TGATGGCGGT TTGCGTCTTG GAGAAATGGA ACGTGACTGT 
TTAATCGGCT ACGGAGCCAG CATGCTCCTA CTAGAGAGAC TGATGATTTC AAGTGATGCC 
TTTGAAGTTG ATGTCTGTGG GCAGTGTGGA CTTCTGGGGT ATTCTGGCTG GTGCCATTAC 
TGCAAGTCAT CATGCCATGT GTCTTCCCTC CGCATCCCAT ATGCCTGCAA GCTGCTCTTC 
CAGGAGCTGC AGTCTATGAA CATCATCCCC CGGTTAAAAC TGTCCAAATA CAATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR3B ( XM_005625950.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005626007.1 Canis lupus familiaris RNA polymerase III subunit B (POLR3B), transcript variant X2, mRNA.

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CloneID	OCe13573
Clone ID Related Accession (Same CDS sequence)	XM_005625950.2 , XM_005625950.3
Accession Version	XM_005625950.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	DNA-directed RNA polymerase III subunit RPC2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625950.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTGC TCGCGGAGGA GTTCGGGAAC CTGACCCCAG AGCAGCTGGC GGCGCCCATC 
CCCACCGTAG AGGAAAAATG GAGGCTACTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG 
AAACAGCATA TAGATTCATT CAACTATTTC ATTAATGTGG AGATAAAGAA GATCATGAAG 
GCCAACGAAA AGGTCACGAG TGATGCCGAC CCCATGTGGT ACCTGAAGTA TCTTAATATC 
TATGTGGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT ATCCCCTCAT 
GAGTGCCGTT TACGGGATAT GACGTACTCT GCCCCCATCA CCGTGGATAT TGAATATACC 
CGAGGCAGCC AGAGGATCAT CCGTAATGCC TTACCAATTG GCAGAATGCC CATAATGCTG 
CGTAGTTCGA ACTGTGTTCT TACAGGGAAG ACACCAGCAG AATTTGCCAA ACTGAACGAA 
TGTCCTTTAG ATCCAGGTGG CTACTTCATT GTCAAAGGAG TAGAAAAAGT AATTCTTATC 
CAAGAGCAGC TGTCTAAGAA CAGGATCATT GTGGAGGCTG ACAGGAAAGG GACGGTTGGT 
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAG 
GGGCGATTTT ATTTGAGACA TAATACTCTG TCAGAAGATA TTCCCATTGT CATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTACAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGTTTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG 
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGGAGGC AAAGGATGTG GGGCGGTGGA 
CCAAAGAAGA CGAAGATAGA AGAGGCAAGA GAGCTCCTGG CTTCTACCAT CCTCACACAC 
GTGCCAGTTA AAGAATTCAA TTTCCGAGCG AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGAGTAATTC TGGCCCAAGG AGATAATAAA GTGGATGACC GAGACTATTA TGGTAACAAG 
CGATTGGAGT TGGCAGGACA GGGAAATTGG TCTCTCAAGA GATTTAAAAT GGACCGCCAG 
GGTGTGACTC AAGTCTTGTC TCGCTTGTCA TATATATCTG CCCTGGGCAT GATGACAAGA 
ATCTCCTCTC AGTTTGAAAA AACAAGAAAA GTGAGTGGTC CTCGTTCCCT CCAGCCATCT 
CAGTGGGGAA TGCTCTGTCC CTCGGACACT CCTGAAGGAG AGGCATGTGG TTTGGTTAAA 
AACTTGGCCC TTATGACGCA CATCACAACT GACATGGAAG ATGGACCCAT TGTTAAATTA 
GCCAGTAACC TGGGGGTAGA AGATGTGAAT TTATTATGTG GGGAAGAACT CTCTTATCCA 
AATGTGTTTC TCGTCTTCCT CAATGGTAAC ATCCTGGGTG TGATTCGAGA CCACAAGAAG 
CTAGTGAATA CATTTCGACT GATGCGAAGA GCAGGCTATA TCAATGAATT TGTTTCCATC 
TCAACAAATC TCACAGATCG ATGTGTCTAT ATTTCCTCTG ATGGAGGAAG GCTTTGCAGA 
CCCTACATTA TCGTTAAGAA ACAGAAGCCT GCAGTCACAA ATAAGCATAT GGAAGAGCTG 
GCTCAGGGAT ACAGGAATTT TGAAGACTTC TTACATGAGA GTCTGGTTGA ATATTTAGAT 
GTGAATGAAG AAAATGACTG TAACATCGCA CTCTATGAAC ATACAATTAA TAAGGATACC 
ACCCACTTGG AGATTGAGCC CTTTACTCTC CTTGGTGTTT GTGCCGGCCT CATCCCATAC 
CCTCATCACA ACCAGTCCCC AAGAAACACA TATCAGTGTG CCATGGGGAA GCAAGCCATG 
GGTACCATTG GATACAACCA GCGGAACAGA ATCGATACTC TCATGTATCT ACTAGCATAC 
CCACAAAAAC CCATGGTTAA AACAAAGACC ATTGAGTTGA TAGAATTTGA GAAACTGCCA 
GCTGGACAAA ATGCAACAGT TGCCGTGATG AGTTACAGTG GCTATGATAT TGAAGATGCC 
CTGGTTTTAA ACAAGGCGTC CCTGGACAGG GGCTTTGGGC GTTGTCTTGT ATATAAAAAT 
GCTAAATGTA CACTGAAACG CTATACCAAT CAGACCTTTG ATAAAGTGAT GGGGCCGATG 
TTGGATGCTG CTACAAGGAA ACCCATCTGG CGACATGAAA TTTTAGATGC GGATGGTATT 
TGTTCTCCAG GTGAGAAGGT AGAAAACAAA CAAGTGCTGG TAAATAAGTC CATGCCCACA 
GTAACCCAGA TTCCTTTGGA AGGAAGTAAT GTGCCACAGC AGCCACAGTA CAAAGATGTG 
CCCATCACCT ACAAAGGAGC AACGGATTCC TACATTGAAA AAGTGATGAT CTCTTCAAAT 
GCTGAAGATG CTTTTCTGAT CAAAATGCTG CTGAGACAGA CCAGGCGTCC AGAGATTGGA 
GACAAATTCA GCAGCCGTCA TGGGCAAAAA GGTGTTTGCG GCTTGATCGT CCCGCAGGAA 
GACATGCCAT TCTGTGATTC TGGCATCTGT CCGGACATCA TAATGAACCC ACACGGCTTC 
CCATCACGAA TGACGGTGGG AAAGCTGATT GAGCTGTTGG CGGGCAAGGC TGGCGTGCTG 
GACGGGAGGT TCCACTATGG CACTGCGTTT GGAGGCAGCA AAGTGAAAGA TGTGTGTGAA 
GACCTCGTTC GCCATGGTTA TAACTACTTG GGGAAAGACT TTGTGACATC TGGCATTACA 
GGCGAGCCCT TAGAAGCATA CATCTATTTT GGCCCGGTGT ACTATCAGAA ACTGAAGCAC 
ATGGTGTTGG ATAAGATGCA TGCCCGAGCC CGGGGGCCAC GAGCTGTTCT CACCAGGCAG 
CCCACTGAAG GTCGGTCTCG TGATGGCGGT TTGCGTCTTG GAGAAATGGA ACGTGACTGT 
TTAATCGGCT ACGGAGCCAG CATGCTCCTA CTAGAGAGAC TGATGATTTC AAGTGATGCC 
TTTGAAGTTG ATGTCTGTGG GCAGTGTGGA CTTCTGGGGT ATTCTGGCTG GTGCCATTAC 
TGCAAGTCAT CATGCCATGT GTCTTCCCTC CGCATCCCAT ATGCCTGCAA GCTGCTCTTC 
CAGGAGCTGC AGTCTATGAA CATCATCCCC CGGTTAAAAC TGTCCAAATA CAATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626007.1
CDS	162..3401
Translation	MDVLAEEFGNLTPEQLAAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGTVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFRLMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHGYNYLGKDFVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLSKYNE

Target ORF information:

RefSeq Version	XM_005625950.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RNA polymerase III subunit B (POLR3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625950.3

ORF Insert Sequence:

ATGGATGTGC TCGCGGAGGA GTTCGGGAAC CTGACCCCAG AGCAGCTGGC GGCGCCCATC 
CCCACCGTAG AGGAAAAATG GAGGCTACTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG 
AAACAGCATA TAGATTCATT CAACTATTTC ATTAATGTGG AGATAAAGAA GATCATGAAG 
GCCAACGAAA AGGTCACGAG TGATGCCGAC CCCATGTGGT ACCTGAAGTA TCTTAATATC 
TATGTGGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT ATCCCCTCAT 
GAGTGCCGTT TACGGGATAT GACGTACTCT GCCCCCATCA CCGTGGATAT TGAATATACC 
CGAGGCAGCC AGAGGATCAT CCGTAATGCC TTACCAATTG GCAGAATGCC CATAATGCTG 
CGTAGTTCGA ACTGTGTTCT TACAGGGAAG ACACCAGCAG AATTTGCCAA ACTGAACGAA 
TGTCCTTTAG ATCCAGGTGG CTACTTCATT GTCAAAGGAG TAGAAAAAGT AATTCTTATC 
CAAGAGCAGC TGTCTAAGAA CAGGATCATT GTGGAGGCTG ACAGGAAAGG GACGGTTGGT 
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAG 
GGGCGATTTT ATTTGAGACA TAATACTCTG TCAGAAGATA TTCCCATTGT CATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTACAGA TGATTGGAAC AGAGGAGCAC 
GTGATGGCTG CATTTGGGCC CAGTTTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG 
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGGAGGC AAAGGATGTG GGGCGGTGGA 
CCAAAGAAGA CGAAGATAGA AGAGGCAAGA GAGCTCCTGG CTTCTACCAT CCTCACACAC 
GTGCCAGTTA AAGAATTCAA TTTCCGAGCG AAGTGTATCT ACACTGCAGT GATGGTGCGA 
AGAGTAATTC TGGCCCAAGG AGATAATAAA GTGGATGACC GAGACTATTA TGGTAACAAG 
CGATTGGAGT TGGCAGGACA GGGAAATTGG TCTCTCAAGA GATTTAAAAT GGACCGCCAG 
GGTGTGACTC AAGTCTTGTC TCGCTTGTCA TATATATCTG CCCTGGGCAT GATGACAAGA 
ATCTCCTCTC AGTTTGAAAA AACAAGAAAA GTGAGTGGTC CTCGTTCCCT CCAGCCATCT 
CAGTGGGGAA TGCTCTGTCC CTCGGACACT CCTGAAGGAG AGGCATGTGG TTTGGTTAAA 
AACTTGGCCC TTATGACGCA CATCACAACT GACATGGAAG ATGGACCCAT TGTTAAATTA 
GCCAGTAACC TGGGGGTAGA AGATGTGAAT TTATTATGTG GGGAAGAACT CTCTTATCCA 
AATGTGTTTC TCGTCTTCCT CAATGGTAAC ATCCTGGGTG TGATTCGAGA CCACAAGAAG 
CTAGTGAATA CATTTCGACT GATGCGAAGA GCAGGCTATA TCAATGAATT TGTTTCCATC 
TCAACAAATC TCACAGATCG ATGTGTCTAT ATTTCCTCTG ATGGAGGAAG GCTTTGCAGA 
CCCTACATTA TCGTTAAGAA ACAGAAGCCT GCAGTCACAA ATAAGCATAT GGAAGAGCTG 
GCTCAGGGAT ACAGGAATTT TGAAGACTTC TTACATGAGA GTCTGGTTGA ATATTTAGAT 
GTGAATGAAG AAAATGACTG TAACATCGCA CTCTATGAAC ATACAATTAA TAAGGATACC 
ACCCACTTGG AGATTGAGCC CTTTACTCTC CTTGGTGTTT GTGCCGGCCT CATCCCATAC 
CCTCATCACA ACCAGTCCCC AAGAAACACA TATCAGTGTG CCATGGGGAA GCAAGCCATG 
GGTACCATTG GATACAACCA GCGGAACAGA ATCGATACTC TCATGTATCT ACTAGCATAC 
CCACAAAAAC CCATGGTTAA AACAAAGACC ATTGAGTTGA TAGAATTTGA GAAACTGCCA 
GCTGGACAAA ATGCAACAGT TGCCGTGATG AGTTACAGTG GCTATGATAT TGAAGATGCC 
CTGGTTTTAA ACAAGGCGTC CCTGGACAGG GGCTTTGGGC GTTGTCTTGT ATATAAAAAT 
GCTAAATGTA CACTGAAACG CTATACCAAT CAGACCTTTG ATAAAGTGAT GGGGCCGATG 
TTGGATGCTG CTACAAGGAA ACCCATCTGG CGACATGAAA TTTTAGATGC GGATGGTATT 
TGTTCTCCAG GTGAGAAGGT AGAAAACAAA CAAGTGCTGG TAAATAAGTC CATGCCCACA 
GTAACCCAGA TTCCTTTGGA AGGAAGTAAT GTGCCACAGC AGCCACAGTA CAAAGATGTG 
CCCATCACCT ACAAAGGAGC AACGGATTCC TACATTGAAA AAGTGATGAT CTCTTCAAAT 
GCTGAAGATG CTTTTCTGAT CAAAATGCTG CTGAGACAGA CCAGGCGTCC AGAGATTGGA 
GACAAATTCA GCAGCCGTCA TGGGCAAAAA GGTGTTTGCG GCTTGATCGT CCCGCAGGAA 
GACATGCCAT TCTGTGATTC TGGCATCTGT CCGGACATCA TAATGAACCC ACACGGCTTC 
CCATCACGAA TGACGGTGGG AAAGCTGATT GAGCTGTTGG CGGGCAAGGC TGGCGTGCTG 
GACGGGAGGT TCCACTATGG CACTGCGTTT GGAGGCAGCA AAGTGAAAGA TGTGTGTGAA 
GACCTCGTTC GCCATGGTTA TAACTACTTG GGGAAAGACT TTGTGACATC TGGCATTACA 
GGCGAGCCCT TAGAAGCATA CATCTATTTT GGCCCGGTGT ACTATCAGAA ACTGAAGCAC 
ATGGTGTTGG ATAAGATGCA TGCCCGAGCC CGGGGGCCAC GAGCTGTTCT CACCAGGCAG 
CCCACTGAAG GTCGGTCTCG TGATGGCGGT TTGCGTCTTG GAGAAATGGA ACGTGACTGT 
TTAATCGGCT ACGGAGCCAG CATGCTCCTA CTAGAGAGAC TGATGATTTC AAGTGATGCC 
TTTGAAGTTG ATGTCTGTGG GCAGTGTGGA CTTCTGGGGT ATTCTGGCTG GTGCCATTAC 
TGCAAGTCAT CATGCCATGT GTCTTCCCTC CGCATCCCAT ATGCCTGCAA GCTGCTCTTC 
CAGGAGCTGC AGTCTATGAA CATCATCCCC CGGTTAAAAC TGTCCAAATA CAATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR3B ( XM_005625951.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005626008.1 Canis lupus familiaris polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe13574
Clone ID Related Accession (Same CDS sequence)	XM_005625951.2 , XM_005625951.3
Accession Version	XM_005625951.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	DNA-directed RNA polymerase III subunit RPC2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCCA ACGAAAAGGT CACGAGTGAT GCCGACCCCA TGTGGTACCT GAAGTATCTT 
AATATCTATG TGGGGCTTCC TGATGTTGAA GAAAGCTTCA ATGTAACTAG ACCAGTATCC 
CCTCATGAGT GCCGTTTACG GGATATGACG TACTCTGCCC CCATCACCGT GGATATTGAA 
TATACCCGAG GCAGCCAGAG GATCATCCGT AATGCCTTAC CAATTGGCAG AATGCCCATA 
ATGCTGCGTA GTTCGAACTG TGTTCTTACA GGGAAGACAC CAGCAGAATT TGCCAAACTG 
AACGAATGTC CTTTAGATCC AGGTGGCTAC TTCATTGTCA AAGGAGTAGA AAAAGTAATT 
CTTATCCAAG AGCAGCTGTC TAAGAACAGG ATCATTGTGG AGGCTGACAG GAAAGGGACG 
GTTGGTGCTT CAGTTACCAG CTCTACCCAT GAGAAAAAAA GCAGAACCAA TATGGCTGTG 
AAACAGGGGC GATTTTATTT GAGACATAAT ACTCTGTCAG AAGATATTCC CATTGTCATC 
ATATTTAAGG CCATGGGTGT TGAGAGTGAC CAGGAAATTG TACAGATGAT TGGAACAGAG 
GAGCACGTGA TGGCTGCATT TGGGCCCAGT TTGGAAGAGT GCCAGAAAGC TCAGATTTTC 
ACACAGATGC AGGCATTAAA ATATATAGGG AACAAAGTAA GGAGGCAAAG GATGTGGGGC 
GGTGGACCAA AGAAGACGAA GATAGAAGAG GCAAGAGAGC TCCTGGCTTC TACCATCCTC 
ACACACGTGC CAGTTAAAGA ATTCAATTTC CGAGCGAAGT GTATCTACAC TGCAGTGATG 
GTGCGAAGAG TAATTCTGGC CCAAGGAGAT AATAAAGTGG ATGACCGAGA CTATTATGGT 
AACAAGCGAT TGGAGTTGGC AGGACAGCTT TTATCACTTC TTTTTGAAGA TTTATTCAAA 
AAATTTAATT CTGAAATGAA GAAGATTGCA GACCAGGTGA TTCCTAAGCA AAGAGCAGCT 
CAGTTTGATG TTGTGAAACA CATGCGTCAG GACCAGATTA CCAACGGCAT GGTGAATGCC 
ATTTCTACAG GAAATTGGTC TCTCAAGAGA TTTAAAATGG ACCGCCAGGG TGTGACTCAA 
GTCTTGTCTC GCTTGTCATA TATATCTGCC CTGGGCATGA TGACAAGAAT CTCCTCTCAG 
TTTGAAAAAA CAAGAAAAGT GAGTGGTCCT CGTTCCCTCC AGCCATCTCA GTGGGGAATG 
CTCTGTCCCT CGGACACTCC TGAAGGAGAG GCATGTGGTT TGGTTAAAAA CTTGGCCCTT 
ATGACGCACA TCACAACTGA CATGGAAGAT GGACCCATTG TTAAATTAGC CAGTAACCTG 
GGGGTAGAAG ATGTGAATTT ATTATGTGGG GAAGAACTCT CTTATCCAAA TGTGTTTCTC 
GTCTTCCTCA ATGGTAACAT CCTGGGTGTG ATTCGAGACC ACAAGAAGCT AGTGAATACA 
TTTCGACTGA TGCGAAGAGC AGGCTATATC AATGAATTTG TTTCCATCTC AACAAATCTC 
ACAGATCGAT GTGTCTATAT TTCCTCTGAT GGAGGAAGGC TTTGCAGACC CTACATTATC 
GTTAAGAAAC AGAAGCCTGC AGTCACAAAT AAGCATATGG AAGAGCTGGC TCAGGGATAC 
AGGAATTTTG AAGACTTCTT ACATGAGAGT CTGGTTGAAT ATTTAGATGT GAATGAAGAA 
AATGACTGTA ACATCGCACT CTATGAACAT ACAATTAATA AGGATACCAC CCACTTGGAG 
ATTGAGCCCT TTACTCTCCT TGGTGTTTGT GCCGGCCTCA TCCCATACCC TCATCACAAC 
CAGTCCCCAA GAAACACATA TCAGTGTGCC ATGGGGAAGC AAGCCATGGG TACCATTGGA 
TACAACCAGC GGAACAGAAT CGATACTCTC ATGTATCTAC TAGCATACCC ACAAAAACCC 
ATGGTTAAAA CAAAGACCAT TGAGTTGATA GAATTTGAGA AACTGCCAGC TGGACAAAAT 
GCAACAGTTG CCGTGATGAG TTACAGTGGC TATGATATTG AAGATGCCCT GGTTTTAAAC 
AAGGCGTCCC TGGACAGGGG CTTTGGGCGT TGTCTTGTAT ATAAAAATGC TAAATGTACA 
CTGAAACGCT ATACCAATCA GACCTTTGAT AAAGTGATGG GGCCGATGTT GGATGCTGCT 
ACAAGGAAAC CCATCTGGCG ACATGAAATT TTAGATGCGG ATGGTATTTG TTCTCCAGGT 
GAGAAGGTAG AAAACAAACA AGTGCTGGTA AATAAGTCCA TGCCCACAGT AACCCAGATT 
CCTTTGGAAG GAAGTAATGT GCCACAGCAG CCACAGTACA AAGATGTGCC CATCACCTAC 
AAAGGAGCAA CGGATTCCTA CATTGAAAAA GTGATGATCT CTTCAAATGC TGAAGATGCT 
TTTCTGATCA AAATGCTGCT GAGACAGACC AGGCGTCCAG AGATTGGAGA CAAATTCAGC 
AGCCGTCATG GGCAAAAAGG TGTTTGCGGC TTGATCGTCC CGCAGGAAGA CATGCCATTC 
TGTGATTCTG GCATCTGTCC GGACATCATA ATGAACCCAC ACGGCTTCCC ATCACGAATG 
ACGGTGGGAA AGCTGATTGA GCTGTTGGCG GGCAAGGCTG GCGTGCTGGA CGGGAGGTTC 
CACTATGGCA CTGCGTTTGG AGGCAGCAAA GTGAAAGATG TGTGTGAAGA CCTCGTTCGC 
CATGGTTATA ACTACTTGGG GAAAGACTTT GTGACATCTG GCATTACAGG CGAGCCCTTA 
GAAGCATACA TCTATTTTGG CCCGGTGTAC TATCAGAAAC TGAAGCACAT GGTGTTGGAT 
AAGATGCATG CCCGAGCCCG GGGGCCACGA GCTGTTCTCA CCAGGCAGCC CACTGAAGGT 
CGGTCTCGTG ATGGCGGTTT GCGTCTTGGA GAAATGGAAC GTGACTGTTT AATCGGCTAC 
GGAGCCAGCA TGCTCCTACT AGAGAGACTG ATGATTTCAA GTGATGCCTT TGAAGTTGAT 
GTCTGTGGGC AGTGTGGACT TCTGGGGTAT TCTGGCTGGT GCCATTACTG CAAGTCATCA 
TGCCATGTGT CTTCCCTCCG CATCCCATAT GCCTGCAAGC TGCTCTTCCA GGAGCTGCAG 
TCTATGAACA TCATCCCCCG GTTAAAACTG TCCAAATACA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626008.1
CDS	340..3567
Translation	MKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGTVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFRLMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHGYNYLGKDFVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLSKYNE

Target ORF information:

RefSeq Version	XM_005625951.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625951.2

ORF Insert Sequence:

ATGAAGGCCA ACGAAAAGGT CACGAGTGAT GCCGACCCCA TGTGGTACCT GAAGTATCTT 
AATATCTATG TGGGGCTTCC TGATGTTGAA GAAAGCTTCA ATGTAACTAG ACCAGTATCC 
CCTCATGAGT GCCGTTTACG GGATATGACG TACTCTGCCC CCATCACCGT GGATATTGAA 
TATACCCGAG GCAGCCAGAG GATCATCCGT AATGCCTTAC CAATTGGCAG AATGCCCATA 
ATGCTGCGTA GTTCGAACTG TGTTCTTACA GGGAAGACAC CAGCAGAATT TGCCAAACTG 
AACGAATGTC CTTTAGATCC AGGTGGCTAC TTCATTGTCA AAGGAGTAGA AAAAGTAATT 
CTTATCCAAG AGCAGCTGTC TAAGAACAGG ATCATTGTGG AGGCTGACAG GAAAGGGACG 
GTTGGTGCTT CAGTTACCAG CTCTACCCAT GAGAAAAAAA GCAGAACCAA TATGGCTGTG 
AAACAGGGGC GATTTTATTT GAGACATAAT ACTCTGTCAG AAGATATTCC CATTGTCATC 
ATATTTAAGG CCATGGGTGT TGAGAGTGAC CAGGAAATTG TACAGATGAT TGGAACAGAG 
GAGCACGTGA TGGCTGCATT TGGGCCCAGT TTGGAAGAGT GCCAGAAAGC TCAGATTTTC 
ACACAGATGC AGGCATTAAA ATATATAGGG AACAAAGTAA GGAGGCAAAG GATGTGGGGC 
GGTGGACCAA AGAAGACGAA GATAGAAGAG GCAAGAGAGC TCCTGGCTTC TACCATCCTC 
ACACACGTGC CAGTTAAAGA ATTCAATTTC CGAGCGAAGT GTATCTACAC TGCAGTGATG 
GTGCGAAGAG TAATTCTGGC CCAAGGAGAT AATAAAGTGG ATGACCGAGA CTATTATGGT 
AACAAGCGAT TGGAGTTGGC AGGACAGCTT TTATCACTTC TTTTTGAAGA TTTATTCAAA 
AAATTTAATT CTGAAATGAA GAAGATTGCA GACCAGGTGA TTCCTAAGCA AAGAGCAGCT 
CAGTTTGATG TTGTGAAACA CATGCGTCAG GACCAGATTA CCAACGGCAT GGTGAATGCC 
ATTTCTACAG GAAATTGGTC TCTCAAGAGA TTTAAAATGG ACCGCCAGGG TGTGACTCAA 
GTCTTGTCTC GCTTGTCATA TATATCTGCC CTGGGCATGA TGACAAGAAT CTCCTCTCAG 
TTTGAAAAAA CAAGAAAAGT GAGTGGTCCT CGTTCCCTCC AGCCATCTCA GTGGGGAATG 
CTCTGTCCCT CGGACACTCC TGAAGGAGAG GCATGTGGTT TGGTTAAAAA CTTGGCCCTT 
ATGACGCACA TCACAACTGA CATGGAAGAT GGACCCATTG TTAAATTAGC CAGTAACCTG 
GGGGTAGAAG ATGTGAATTT ATTATGTGGG GAAGAACTCT CTTATCCAAA TGTGTTTCTC 
GTCTTCCTCA ATGGTAACAT CCTGGGTGTG ATTCGAGACC ACAAGAAGCT AGTGAATACA 
TTTCGACTGA TGCGAAGAGC AGGCTATATC AATGAATTTG TTTCCATCTC AACAAATCTC 
ACAGATCGAT GTGTCTATAT TTCCTCTGAT GGAGGAAGGC TTTGCAGACC CTACATTATC 
GTTAAGAAAC AGAAGCCTGC AGTCACAAAT AAGCATATGG AAGAGCTGGC TCAGGGATAC 
AGGAATTTTG AAGACTTCTT ACATGAGAGT CTGGTTGAAT ATTTAGATGT GAATGAAGAA 
AATGACTGTA ACATCGCACT CTATGAACAT ACAATTAATA AGGATACCAC CCACTTGGAG 
ATTGAGCCCT TTACTCTCCT TGGTGTTTGT GCCGGCCTCA TCCCATACCC TCATCACAAC 
CAGTCCCCAA GAAACACATA TCAGTGTGCC ATGGGGAAGC AAGCCATGGG TACCATTGGA 
TACAACCAGC GGAACAGAAT CGATACTCTC ATGTATCTAC TAGCATACCC ACAAAAACCC 
ATGGTTAAAA CAAAGACCAT TGAGTTGATA GAATTTGAGA AACTGCCAGC TGGACAAAAT 
GCAACAGTTG CCGTGATGAG TTACAGTGGC TATGATATTG AAGATGCCCT GGTTTTAAAC 
AAGGCGTCCC TGGACAGGGG CTTTGGGCGT TGTCTTGTAT ATAAAAATGC TAAATGTACA 
CTGAAACGCT ATACCAATCA GACCTTTGAT AAAGTGATGG GGCCGATGTT GGATGCTGCT 
ACAAGGAAAC CCATCTGGCG ACATGAAATT TTAGATGCGG ATGGTATTTG TTCTCCAGGT 
GAGAAGGTAG AAAACAAACA AGTGCTGGTA AATAAGTCCA TGCCCACAGT AACCCAGATT 
CCTTTGGAAG GAAGTAATGT GCCACAGCAG CCACAGTACA AAGATGTGCC CATCACCTAC 
AAAGGAGCAA CGGATTCCTA CATTGAAAAA GTGATGATCT CTTCAAATGC TGAAGATGCT 
TTTCTGATCA AAATGCTGCT GAGACAGACC AGGCGTCCAG AGATTGGAGA CAAATTCAGC 
AGCCGTCATG GGCAAAAAGG TGTTTGCGGC TTGATCGTCC CGCAGGAAGA CATGCCATTC 
TGTGATTCTG GCATCTGTCC GGACATCATA ATGAACCCAC ACGGCTTCCC ATCACGAATG 
ACGGTGGGAA AGCTGATTGA GCTGTTGGCG GGCAAGGCTG GCGTGCTGGA CGGGAGGTTC 
CACTATGGCA CTGCGTTTGG AGGCAGCAAA GTGAAAGATG TGTGTGAAGA CCTCGTTCGC 
CATGGTTATA ACTACTTGGG GAAAGACTTT GTGACATCTG GCATTACAGG CGAGCCCTTA 
GAAGCATACA TCTATTTTGG CCCGGTGTAC TATCAGAAAC TGAAGCACAT GGTGTTGGAT 
AAGATGCATG CCCGAGCCCG GGGGCCACGA GCTGTTCTCA CCAGGCAGCC CACTGAAGGT 
CGGTCTCGTG ATGGCGGTTT GCGTCTTGGA GAAATGGAAC GTGACTGTTT AATCGGCTAC 
GGAGCCAGCA TGCTCCTACT AGAGAGACTG ATGATTTCAA GTGATGCCTT TGAAGTTGAT 
GTCTGTGGGC AGTGTGGACT TCTGGGGTAT TCTGGCTGGT GCCATTACTG CAAGTCATCA 
TGCCATGTGT CTTCCCTCCG CATCCCATAT GCCTGCAAGC TGCTCTTCCA GGAGCTGCAG 
TCTATGAACA TCATCCCCCG GTTAAAACTG TCCAAATACA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR3B ( XM_005625951.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005626008.1 Canis lupus familiaris RNA polymerase III subunit B (POLR3B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe13574
Clone ID Related Accession (Same CDS sequence)	XM_005625951.2 , XM_005625951.3
Accession Version	XM_005625951.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	DNA-directed RNA polymerase III subunit RPC2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCCA ACGAAAAGGT CACGAGTGAT GCCGACCCCA TGTGGTACCT GAAGTATCTT 
AATATCTATG TGGGGCTTCC TGATGTTGAA GAAAGCTTCA ATGTAACTAG ACCAGTATCC 
CCTCATGAGT GCCGTTTACG GGATATGACG TACTCTGCCC CCATCACCGT GGATATTGAA 
TATACCCGAG GCAGCCAGAG GATCATCCGT AATGCCTTAC CAATTGGCAG AATGCCCATA 
ATGCTGCGTA GTTCGAACTG TGTTCTTACA GGGAAGACAC CAGCAGAATT TGCCAAACTG 
AACGAATGTC CTTTAGATCC AGGTGGCTAC TTCATTGTCA AAGGAGTAGA AAAAGTAATT 
CTTATCCAAG AGCAGCTGTC TAAGAACAGG ATCATTGTGG AGGCTGACAG GAAAGGGACG 
GTTGGTGCTT CAGTTACCAG CTCTACCCAT GAGAAAAAAA GCAGAACCAA TATGGCTGTG 
AAACAGGGGC GATTTTATTT GAGACATAAT ACTCTGTCAG AAGATATTCC CATTGTCATC 
ATATTTAAGG CCATGGGTGT TGAGAGTGAC CAGGAAATTG TACAGATGAT TGGAACAGAG 
GAGCACGTGA TGGCTGCATT TGGGCCCAGT TTGGAAGAGT GCCAGAAAGC TCAGATTTTC 
ACACAGATGC AGGCATTAAA ATATATAGGG AACAAAGTAA GGAGGCAAAG GATGTGGGGC 
GGTGGACCAA AGAAGACGAA GATAGAAGAG GCAAGAGAGC TCCTGGCTTC TACCATCCTC 
ACACACGTGC CAGTTAAAGA ATTCAATTTC CGAGCGAAGT GTATCTACAC TGCAGTGATG 
GTGCGAAGAG TAATTCTGGC CCAAGGAGAT AATAAAGTGG ATGACCGAGA CTATTATGGT 
AACAAGCGAT TGGAGTTGGC AGGACAGCTT TTATCACTTC TTTTTGAAGA TTTATTCAAA 
AAATTTAATT CTGAAATGAA GAAGATTGCA GACCAGGTGA TTCCTAAGCA AAGAGCAGCT 
CAGTTTGATG TTGTGAAACA CATGCGTCAG GACCAGATTA CCAACGGCAT GGTGAATGCC 
ATTTCTACAG GAAATTGGTC TCTCAAGAGA TTTAAAATGG ACCGCCAGGG TGTGACTCAA 
GTCTTGTCTC GCTTGTCATA TATATCTGCC CTGGGCATGA TGACAAGAAT CTCCTCTCAG 
TTTGAAAAAA CAAGAAAAGT GAGTGGTCCT CGTTCCCTCC AGCCATCTCA GTGGGGAATG 
CTCTGTCCCT CGGACACTCC TGAAGGAGAG GCATGTGGTT TGGTTAAAAA CTTGGCCCTT 
ATGACGCACA TCACAACTGA CATGGAAGAT GGACCCATTG TTAAATTAGC CAGTAACCTG 
GGGGTAGAAG ATGTGAATTT ATTATGTGGG GAAGAACTCT CTTATCCAAA TGTGTTTCTC 
GTCTTCCTCA ATGGTAACAT CCTGGGTGTG ATTCGAGACC ACAAGAAGCT AGTGAATACA 
TTTCGACTGA TGCGAAGAGC AGGCTATATC AATGAATTTG TTTCCATCTC AACAAATCTC 
ACAGATCGAT GTGTCTATAT TTCCTCTGAT GGAGGAAGGC TTTGCAGACC CTACATTATC 
GTTAAGAAAC AGAAGCCTGC AGTCACAAAT AAGCATATGG AAGAGCTGGC TCAGGGATAC 
AGGAATTTTG AAGACTTCTT ACATGAGAGT CTGGTTGAAT ATTTAGATGT GAATGAAGAA 
AATGACTGTA ACATCGCACT CTATGAACAT ACAATTAATA AGGATACCAC CCACTTGGAG 
ATTGAGCCCT TTACTCTCCT TGGTGTTTGT GCCGGCCTCA TCCCATACCC TCATCACAAC 
CAGTCCCCAA GAAACACATA TCAGTGTGCC ATGGGGAAGC AAGCCATGGG TACCATTGGA 
TACAACCAGC GGAACAGAAT CGATACTCTC ATGTATCTAC TAGCATACCC ACAAAAACCC 
ATGGTTAAAA CAAAGACCAT TGAGTTGATA GAATTTGAGA AACTGCCAGC TGGACAAAAT 
GCAACAGTTG CCGTGATGAG TTACAGTGGC TATGATATTG AAGATGCCCT GGTTTTAAAC 
AAGGCGTCCC TGGACAGGGG CTTTGGGCGT TGTCTTGTAT ATAAAAATGC TAAATGTACA 
CTGAAACGCT ATACCAATCA GACCTTTGAT AAAGTGATGG GGCCGATGTT GGATGCTGCT 
ACAAGGAAAC CCATCTGGCG ACATGAAATT TTAGATGCGG ATGGTATTTG TTCTCCAGGT 
GAGAAGGTAG AAAACAAACA AGTGCTGGTA AATAAGTCCA TGCCCACAGT AACCCAGATT 
CCTTTGGAAG GAAGTAATGT GCCACAGCAG CCACAGTACA AAGATGTGCC CATCACCTAC 
AAAGGAGCAA CGGATTCCTA CATTGAAAAA GTGATGATCT CTTCAAATGC TGAAGATGCT 
TTTCTGATCA AAATGCTGCT GAGACAGACC AGGCGTCCAG AGATTGGAGA CAAATTCAGC 
AGCCGTCATG GGCAAAAAGG TGTTTGCGGC TTGATCGTCC CGCAGGAAGA CATGCCATTC 
TGTGATTCTG GCATCTGTCC GGACATCATA ATGAACCCAC ACGGCTTCCC ATCACGAATG 
ACGGTGGGAA AGCTGATTGA GCTGTTGGCG GGCAAGGCTG GCGTGCTGGA CGGGAGGTTC 
CACTATGGCA CTGCGTTTGG AGGCAGCAAA GTGAAAGATG TGTGTGAAGA CCTCGTTCGC 
CATGGTTATA ACTACTTGGG GAAAGACTTT GTGACATCTG GCATTACAGG CGAGCCCTTA 
GAAGCATACA TCTATTTTGG CCCGGTGTAC TATCAGAAAC TGAAGCACAT GGTGTTGGAT 
AAGATGCATG CCCGAGCCCG GGGGCCACGA GCTGTTCTCA CCAGGCAGCC CACTGAAGGT 
CGGTCTCGTG ATGGCGGTTT GCGTCTTGGA GAAATGGAAC GTGACTGTTT AATCGGCTAC 
GGAGCCAGCA TGCTCCTACT AGAGAGACTG ATGATTTCAA GTGATGCCTT TGAAGTTGAT 
GTCTGTGGGC AGTGTGGACT TCTGGGGTAT TCTGGCTGGT GCCATTACTG CAAGTCATCA 
TGCCATGTGT CTTCCCTCCG CATCCCATAT GCCTGCAAGC TGCTCTTCCA GGAGCTGCAG 
TCTATGAACA TCATCCCCCG GTTAAAACTG TCCAAATACA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626008.1
CDS	254..3481
Translation	MKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGTVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFRLMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHGYNYLGKDFVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLSKYNE

Target ORF information:

RefSeq Version	XM_005625951.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RNA polymerase III subunit B (POLR3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625951.3

ORF Insert Sequence:

ATGAAGGCCA ACGAAAAGGT CACGAGTGAT GCCGACCCCA TGTGGTACCT GAAGTATCTT 
AATATCTATG TGGGGCTTCC TGATGTTGAA GAAAGCTTCA ATGTAACTAG ACCAGTATCC 
CCTCATGAGT GCCGTTTACG GGATATGACG TACTCTGCCC CCATCACCGT GGATATTGAA 
TATACCCGAG GCAGCCAGAG GATCATCCGT AATGCCTTAC CAATTGGCAG AATGCCCATA 
ATGCTGCGTA GTTCGAACTG TGTTCTTACA GGGAAGACAC CAGCAGAATT TGCCAAACTG 
AACGAATGTC CTTTAGATCC AGGTGGCTAC TTCATTGTCA AAGGAGTAGA AAAAGTAATT 
CTTATCCAAG AGCAGCTGTC TAAGAACAGG ATCATTGTGG AGGCTGACAG GAAAGGGACG 
GTTGGTGCTT CAGTTACCAG CTCTACCCAT GAGAAAAAAA GCAGAACCAA TATGGCTGTG 
AAACAGGGGC GATTTTATTT GAGACATAAT ACTCTGTCAG AAGATATTCC CATTGTCATC 
ATATTTAAGG CCATGGGTGT TGAGAGTGAC CAGGAAATTG TACAGATGAT TGGAACAGAG 
GAGCACGTGA TGGCTGCATT TGGGCCCAGT TTGGAAGAGT GCCAGAAAGC TCAGATTTTC 
ACACAGATGC AGGCATTAAA ATATATAGGG AACAAAGTAA GGAGGCAAAG GATGTGGGGC 
GGTGGACCAA AGAAGACGAA GATAGAAGAG GCAAGAGAGC TCCTGGCTTC TACCATCCTC 
ACACACGTGC CAGTTAAAGA ATTCAATTTC CGAGCGAAGT GTATCTACAC TGCAGTGATG 
GTGCGAAGAG TAATTCTGGC CCAAGGAGAT AATAAAGTGG ATGACCGAGA CTATTATGGT 
AACAAGCGAT TGGAGTTGGC AGGACAGCTT TTATCACTTC TTTTTGAAGA TTTATTCAAA 
AAATTTAATT CTGAAATGAA GAAGATTGCA GACCAGGTGA TTCCTAAGCA AAGAGCAGCT 
CAGTTTGATG TTGTGAAACA CATGCGTCAG GACCAGATTA CCAACGGCAT GGTGAATGCC 
ATTTCTACAG GAAATTGGTC TCTCAAGAGA TTTAAAATGG ACCGCCAGGG TGTGACTCAA 
GTCTTGTCTC GCTTGTCATA TATATCTGCC CTGGGCATGA TGACAAGAAT CTCCTCTCAG 
TTTGAAAAAA CAAGAAAAGT GAGTGGTCCT CGTTCCCTCC AGCCATCTCA GTGGGGAATG 
CTCTGTCCCT CGGACACTCC TGAAGGAGAG GCATGTGGTT TGGTTAAAAA CTTGGCCCTT 
ATGACGCACA TCACAACTGA CATGGAAGAT GGACCCATTG TTAAATTAGC CAGTAACCTG 
GGGGTAGAAG ATGTGAATTT ATTATGTGGG GAAGAACTCT CTTATCCAAA TGTGTTTCTC 
GTCTTCCTCA ATGGTAACAT CCTGGGTGTG ATTCGAGACC ACAAGAAGCT AGTGAATACA 
TTTCGACTGA TGCGAAGAGC AGGCTATATC AATGAATTTG TTTCCATCTC AACAAATCTC 
ACAGATCGAT GTGTCTATAT TTCCTCTGAT GGAGGAAGGC TTTGCAGACC CTACATTATC 
GTTAAGAAAC AGAAGCCTGC AGTCACAAAT AAGCATATGG AAGAGCTGGC TCAGGGATAC 
AGGAATTTTG AAGACTTCTT ACATGAGAGT CTGGTTGAAT ATTTAGATGT GAATGAAGAA 
AATGACTGTA ACATCGCACT CTATGAACAT ACAATTAATA AGGATACCAC CCACTTGGAG 
ATTGAGCCCT TTACTCTCCT TGGTGTTTGT GCCGGCCTCA TCCCATACCC TCATCACAAC 
CAGTCCCCAA GAAACACATA TCAGTGTGCC ATGGGGAAGC AAGCCATGGG TACCATTGGA 
TACAACCAGC GGAACAGAAT CGATACTCTC ATGTATCTAC TAGCATACCC ACAAAAACCC 
ATGGTTAAAA CAAAGACCAT TGAGTTGATA GAATTTGAGA AACTGCCAGC TGGACAAAAT 
GCAACAGTTG CCGTGATGAG TTACAGTGGC TATGATATTG AAGATGCCCT GGTTTTAAAC 
AAGGCGTCCC TGGACAGGGG CTTTGGGCGT TGTCTTGTAT ATAAAAATGC TAAATGTACA 
CTGAAACGCT ATACCAATCA GACCTTTGAT AAAGTGATGG GGCCGATGTT GGATGCTGCT 
ACAAGGAAAC CCATCTGGCG ACATGAAATT TTAGATGCGG ATGGTATTTG TTCTCCAGGT 
GAGAAGGTAG AAAACAAACA AGTGCTGGTA AATAAGTCCA TGCCCACAGT AACCCAGATT 
CCTTTGGAAG GAAGTAATGT GCCACAGCAG CCACAGTACA AAGATGTGCC CATCACCTAC 
AAAGGAGCAA CGGATTCCTA CATTGAAAAA GTGATGATCT CTTCAAATGC TGAAGATGCT 
TTTCTGATCA AAATGCTGCT GAGACAGACC AGGCGTCCAG AGATTGGAGA CAAATTCAGC 
AGCCGTCATG GGCAAAAAGG TGTTTGCGGC TTGATCGTCC CGCAGGAAGA CATGCCATTC 
TGTGATTCTG GCATCTGTCC GGACATCATA ATGAACCCAC ACGGCTTCCC ATCACGAATG 
ACGGTGGGAA AGCTGATTGA GCTGTTGGCG GGCAAGGCTG GCGTGCTGGA CGGGAGGTTC 
CACTATGGCA CTGCGTTTGG AGGCAGCAAA GTGAAAGATG TGTGTGAAGA CCTCGTTCGC 
CATGGTTATA ACTACTTGGG GAAAGACTTT GTGACATCTG GCATTACAGG CGAGCCCTTA 
GAAGCATACA TCTATTTTGG CCCGGTGTAC TATCAGAAAC TGAAGCACAT GGTGTTGGAT 
AAGATGCATG CCCGAGCCCG GGGGCCACGA GCTGTTCTCA CCAGGCAGCC CACTGAAGGT 
CGGTCTCGTG ATGGCGGTTT GCGTCTTGGA GAAATGGAAC GTGACTGTTT AATCGGCTAC 
GGAGCCAGCA TGCTCCTACT AGAGAGACTG ATGATTTCAA GTGATGCCTT TGAAGTTGAT 
GTCTGTGGGC AGTGTGGACT TCTGGGGTAT TCTGGCTGGT GCCATTACTG CAAGTCATCA 
TGCCATGTGT CTTCCCTCCG CATCCCATAT GCCTGCAAGC TGCTCTTCCA GGAGCTGCAG 
TCTATGAACA TCATCCCCCG GTTAAAACTG TCCAAATACA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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POLR3B cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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