Polr3f cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polr3f
Entrez Gene ID	70408
Full Name	polymerase (RNA) III (DNA directed) polypeptide F
Synonyms	2810411G20Rik,3010019O03Rik,3110032A07Rik,RPC39,RPC6
General protein information	Preferred Names polymerase (RNA) III (DNA directed) polypeptide F Names DNA-directed RNA polymerase III subunit RPC6 DNA-directed RNA polymerase III subunit F RNA polymerase III subunit C6
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 G1

mRNA and Protein(s)

mRNA	Protein	Name
NM_029763.3	NP_084039.2	DNA-directed RNA polymerase III subunit RPC6

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0D04532g	XP_453264.1
Homo sapiens (human)	POLR3F	NP_006457.2
Canis lupus familiaris (dog)	POLR3F	XP_857138.2
Rattus norvegicus (Norway rat)	Polr3f	NP_001101254.1
Eremothecium gossypii	AGOS_AER191W	NP_985048.1
Macaca mulatta (Rhesus monkey)	POLR3F	XP_001090177.2
Gallus gallus (chicken)	POLR3F	NP_001186330.1
Saccharomyces cerevisiae (baker's yeast)	RPC34	NP_014400.1
Xenopus tropicalis (tropical clawed frog)	polr3f	NP_989319.1
Schizosaccharomyces pombe (fission yeast)	rpc34	NP_588398.1
Pan troglodytes (chimpanzee)	POLR3F	XP_003316891.1
Bos taurus (cattle)	POLR3F	NP_001033237.1
Drosophila melanogaster (fruit fly)	CG5380	NP_001247237.1
Caenorhabditis elegans (roundworm)	W09C3.4	NP_491427.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011973	XP_320558.3
Mus musculus (house mouse)	Polr3f	NP_084039.2
Danio rerio (zebrafish)	polr3f	NP_957481.1

Gene Ontology
Bibliography

Related articles in PubMed

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Genetic analysis of albuminuria in a cross between C57BL/6J and DBA/2J mice.
Sheehan S, Tsaih SW, King BL, Stanton C, Churchill GA, Paigen B, DiPetrillo K
American journal of physiology. Renal physiology293(5)F1649-56(2007 Nov)

Identification of positional candidate genes for body weight and adiposity in subcongenic mice.
Chiu S, Kim K, Haus KA, Espinal GM, Millon LV, Warden CH
Physiological genomics31(1)75-85(2007 Sep)

Isolation and characterization of monoclonal antibodies directed against subunits of human RNA polymerases I, II, and III.
Jones E, Kimura H, Vigneron M, Wang Z, Roeder RG, Cook PR
Experimental cell research254(1)163-72(2000 Jan)

Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation.
Wang Z, Roeder RG
Genes & development11(10)1315-26(1997 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Polr3f gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr3f cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07439	NM_029763.3 Latest version!	Mus musculus polymerase (RNA) III (DNA directed) polypeptide F (Polr3f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polr3f ( NM_029763.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084039.2 Mus musculus polymerase (RNA) III (DNA directed) polypeptide F (Polr3f), mRNA.

Price & Availability↑

CloneID	OMu07439
Clone ID Related Accession (Same CDS sequence)	NM_029763.3
Accession Version	NM_029763.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase III subunit RPC6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK029286.1 and AK036166.1. On Jul 15, 2005 this sequence version replaced NM_029763.2. ##Evidence-Data-START## Transcript exon combination :: AK029286.1, AK036166.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGG TGAAGGTCAA GGTGCAGCCG CCGGACGCGG ATCCGGTGGA GATAGAAAAC 
AGAATTATAG AATTATGCCA CCAGTTCCCT CATGGAATCA CAGATCAAGT AATTCAGAAC 
GAAATGCCTC ACATTGAAGC CCAGCAGCGG GCAGTGGCCA TCAATAGACT GTTATCCATG 
GGTCAGTTGG ATCTTTTAAG GAGTAACACA GGCCTCTTAT ATAGAATAAA GGATTCTCAG 
AATGCTGGTA AAATGAAGGG GTCCGATAAC CAGGAAAAAC TGGTCTATCA AATCATAGAG 
GATGCAGGGA ATAAAGGAAT ATGGAGCAGA GATATTCGGT ACAAAAGTAA CTTGCCGTTA 
ACAGAAATCA ACAAAATTCT CAAGAATTTG GAAAGTAAAA AGCTAATTAA AGCCGTGAAG 
TCTGTAGCAG CTTCCAAAAA GAAGGTTTAT ATGCTCTATA ACCTGCAGCC AGACCGATCT 
GTGACTGGCG GTGCCTGGTA CAGTGACCAG GATTTTGAAT CTGAATTTGT TGAGGTGCTT 
AATCAACAGT GTTTCAAATT CCTACAAAGC AAGGCAGAGA CAGCAAGAGA AAGTAAACAG 
AATCCAGTGA TACAAAGAAA TAGTTCATTT GCATCGTCAC ATGAAGTCTG GAAGTACATC 
TGTGAGCTGG GCATCAGCAA GGTAGAGTTG TCCATGGAGG ACATTGAAAC CATCTTGAAC 
ACACTCATTT ATGATGGCAA AGTGGAGATG ACTATCATTG CTGCGAAGGA AGGCACTGTT 
GGCAGCGTAG ATGGACACAT GAAACTGTAC AGGGCAGTCA ACCCAATCCT CCCACCTACA 
GGCGTGGTCC GGGCACCCTG TGGACTCTGC CCCGTTTTTG AGGACTGCCA TGAAGGTGGT 
GAGATTTCGC CGTCTAATTG TATTTACATG ACAGAGTGGC TTGAATTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084039.2
CDS	120..1070
Translation	MAEVKVKVQPPDADPVEIENRIIELCHQFPHGITDQVIQNEMPHIEAQQRAVAINRLLSMGQLDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPVIQRNSSFASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYRAVNPILPPTGVVRAPCGLCPVFEDCHEGGEISPSNCIYMTEWLEF

Target ORF information:

RefSeq Version	NM_029763.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) III (DNA directed) polypeptide F (Polr3f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029763.3

ORF Insert Sequence:

ATGGCGGAGG TGAAGGTCAA GGTGCAGCCG CCGGACGCGG ATCCGGTGGA GATAGAAAAC 
AGAATTATAG AATTATGCCA CCAGTTCCCT CATGGAATCA CAGATCAAGT AATTCAGAAC 
GAAATGCCTC ACATTGAAGC CCAGCAGCGG GCAGTGGCCA TCAATAGACT GTTATCCATG 
GGTCAGTTGG ATCTTTTAAG GAGTAACACA GGCCTCTTAT ATAGAATAAA GGATTCTCAG 
AATGCTGGTA AAATGAAGGG GTCCGATAAC CAGGAAAAAC TGGTCTATCA AATCATAGAG 
GATGCAGGGA ATAAAGGAAT ATGGAGCAGA GATATTCGGT ACAAAAGTAA CTTGCCGTTA 
ACAGAAATCA ACAAAATTCT CAAGAATTTG GAAAGTAAAA AGCTAATTAA AGCCGTGAAG 
TCTGTAGCAG CTTCCAAAAA GAAGGTTTAT ATGCTCTATA ACCTGCAGCC AGACCGATCT 
GTGACTGGCG GTGCCTGGTA CAGTGACCAG GATTTTGAAT CTGAATTTGT TGAGGTGCTT 
AATCAACAGT GTTTCAAATT CCTACAAAGC AAGGCAGAGA CAGCAAGAGA AAGTAAACAG 
AATCCAGTGA TACAAAGAAA TAGTTCATTT GCATCGTCAC ATGAAGTCTG GAAGTACATC 
TGTGAGCTGG GCATCAGCAA GGTAGAGTTG TCCATGGAGG ACATTGAAAC CATCTTGAAC 
ACACTCATTT ATGATGGCAA AGTGGAGATG ACTATCATTG CTGCGAAGGA AGGCACTGTT 
GGCAGCGTAG ATGGACACAT GAAACTGTAC AGGGCAGTCA ACCCAATCCT CCCACCTACA 
GGCGTGGTCC GGGCACCCTG TGGACTCTGC CCCGTTTTTG AGGACTGCCA TGAAGGTGGT 
GAGATTTCGC CGTCTAATTG TATTTACATG ACAGAGTGGC TTGAATTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Genetic analysis of albuminuria in a cross between C57BL/6J and DBA/2J mice.
American journal of physiology. Renal physiology293(5)F1649-56(2007 Nov)
Sheehan S,Tsaih SW,King BL,Stanton C,Churchill GA,Paigen B,DiPetrillo K

Identification of positional candidate genes for body weight and adiposity in subcongenic mice.
Physiological genomics31(1)75-85(2007 Sep)
Chiu S,Kim K,Haus KA,Espinal GM,Millon LV,Warden CH

Isolation and characterization of monoclonal antibodies directed against subunits of human RNA polymerases I, II, and III.
Experimental cell research254(1)163-72(2000 Jan)
Jones E,Kimura H,Vigneron M,Wang Z,Roeder RG,Cook PR

Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation.
Genes & development11(10)1315-26(1997 May)
Wang Z,Roeder RG