Pitrm1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pitrm1
Entrez Gene ID	69617
Full Name	pitrilysin metallepetidase 1
Synonyms	2310012C15Rik,AA410010,MP-1,MP1,Ntup1,PreP,mKIAA1104
General protein information	Preferred Names pitrilysin metallepetidase 1 Names presequence protease, mitochondrial nuclear transplantation upregulated protein 1 pitrilysin metalloprotease 1 pitrilysin metalloproteinase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001360106.1	NP_001347035.1	presequence protease, mitochondrial isoform 2
XM_006516509.3	XP_006516572.1	presequence protease, mitochondrial isoform X1
NM_145131.1	NP_660113.1	presequence protease, mitochondrial isoform 1 precursor
XM_006516508.1	XP_006516571.1	presequence protease, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PITRM1	XP_001140483.1
Arabidopsis thaliana (thale cress)	PREP1	NP_188548.2
Schizosaccharomyces pombe (fission yeast)	SPBC119.17	NP_595299.2
Magnaporthe oryzae (rice blast fungus)	MGG_02440	XP_003709229.1
Neurospora crassa	NCU01272	XP_960750.1
Bos taurus (cattle)	PITRM1	XP_002692237.2
Gallus gallus (chicken)	PITRM1	XP_418564.2
Arabidopsis thaliana (thale cress)	PREP2	NP_850962.1
Homo sapiens (human)	PITRM1	NP_055704.2
Canis lupus familiaris (dog)	PITRM1	XP_535200.2
Rattus norvegicus (Norway rat)	Pitrm1	NP_001100833.1
Macaca mulatta (Rhesus monkey)	PITRM1	XP_001102165.1
Drosophila melanogaster (fruit fly)	CG3107	NP_001036470.1
Mus musculus (house mouse)	Pitrm1	NP_660113.1
Danio rerio (zebrafish)	pitrm1	NP_998652.1
Xenopus tropicalis (tropical clawed frog)	pitrm1	NP_001039262.1
Eremothecium gossypii	AGOS_ADR184W	NP_984280.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006616	XP_316646.4

Gene Ontology
Bibliography

Related articles in PubMed

Placentation defects are highly prevalent in embryonic lethal mouse mutants.
Perez-Garcia V, Fineberg E, Wilson R, Murray A, Mazzeo CI, Tudor C, Sienerth A, White JK, Tuck E, Ryder EJ, Gleeson D, Siragher E, Wardle-Jones H, Staudt N, Wali N, Collins J, Geyer S, Busch-Nentwich EM, Galli A, Smith JC, Robertson E, Adams DJ, Weninger WJ, Mohun T, Hemberger M
Nature555(7697)463-468(2018 03)

Defective PITRM1 mitochondrial peptidase is associated with Aβ amyloidotic neurodegeneration.
Brunetti D, Torsvik J, Dallabona C, Teixeira P, Sztromwasser P, Fernandez-Vizarra E, Cerutti R, Reyes A, Preziuso C, D'Amati G, Baruffini E, Goffrini P, Viscomi C, Ferrero I, Boman H, Telstad W, Johansson S, Glaser E, Knappskog PM, Zeviani M, Bindoff LA
EMBO molecular medicine8(3)176-90(2016 Mar)

The Mitochondrial Peptidase Pitrilysin Degrades Islet Amyloid Polypeptide in Beta-Cells.
Guan H, Chow KM, Song E, Verma N, Despa F, Hersh LB
PloS one10(7)e0133263(2015)

The metalloendopeptidase gene Pitrm1 is regulated by hedgehog signaling in the developing mouse limb and is expressed in muscle progenitors.
Town L, McGlinn E, Fiorenza S, Metzis V, Butterfield NC, Richman JM, Wicking C
Developmental dynamics : an official publication of the American Association of Anatomists238(12)3175-84(2009 Dec)

Fine-mapping gene-by-diet interactions on chromosome 13 in a LG/J x SM/J murine model of obesity.
Ehrich TH, Hrbek T, Kenney-Hunt JP, Pletscher LS, Wang B, Semenkovich CF, Cheverud JM
Diabetes54(6)1863-72(2005 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pitrm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pitrm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu48300	XM_006516509.3 Latest version!	Mus musculus pitrilysin metallepetidase 1 (Pitrm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu14511	NM_145131.1 Latest version!	Mus musculus pitrilysin metallepetidase 1 (Pitrm1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu48299	NM_001360106.1 Latest version!	Mus musculus pitrilysin metallepetidase 1 (Pitrm1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu48299	XM_006516508.1 Latest version!	Mus musculus pitrilysin metallepetidase 1 (Pitrm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pitrm1 ( XM_006516509.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516572.1 Mus musculus pitrilysin metallepetidase 1 (Pitrm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu48300
Clone ID Related Accession (Same CDS sequence)	XM_006516509.3
Accession Version	XM_006516509.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	presequence protease, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516509.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCGCT TCAGCGGTCG GCGGGGACTC TGCGCTGTAC AGCGGCTGAG CTGCGGCAGG 
GTACACCACA GAGTATGGAG GGAGAAGAGT GACCAAGCCT GTGAACGAGC TCTACAGTAT 
AAAGTGGGAG AGAAAATCCA CGGGTTCACT GTAAACCAGG TCACTCCTGT CCCCGAGCTG 
TTCCTGACAG CCGTGAAGCT CAGCCATGAC AACACGGGAG CCAGATACCT GCACCTGGCA 
AGGGAAGACA AGAACAACTT ATTCAGTGTG CAGTTCCGCA CAACCCCAAT GGATAGCACT 
GGGGTCCCAC ATGTTCTCGA GCATACGGTC CTGTGCGGCT CTCAGAAGTA CCCGTGCAGA 
GATCCTTTCT TCAAAATGCT CAACAGGTCA CTGTCCACAT TTATGAATGC CATGACAGCC 
AGCGATTACA CGATATATCC GTTTTCCACT CAAAATCCCA AAGATTTTCA GAACCTCCTC 
TCCGTGTATT TGGATGCAAC TTTCTTCCCC TGCTTGAGGG AACTGGACTT CTGGCAGGAA 
GGATGGCGTC TGGAGCATGA GAATCCCCGA GACCCTCAGA CGCCCTTGAT CTTTAAGGGG 
GTCGTCTTCA ACGAGATGAA AGGGGCATTT ACAGACAATG AGAGGATATT CTCCCAGCAC 
CTGCAGAACA AGCTGCTTCC TGACCACACC TACTCCGTGG TTTCTGGAGG GGACCCACTG 
TGCATCCCGG AGCTCACGTG GGAACAGCTG AAACAGTTCC ACGCTACTCA TTATCACCCA 
AGCAATGCCA GGTTCTTCAC TTATGGCAAT TTTCAGCTGG AAGGACACCT GAAACAAATT 
CACGAAGAAG CCCTGAGTAA ATTCCAGAGA TTGGAGCAGA GTACAGCAGT GCCTGCCCAG 
CCGCACTGGG ATAAGCCTAG GGAATTCCAT ATAACATGTG GCCCAGATTC ACTAGCTACG 
GAGACTGCCA AGCAGACAAC TGTCAGCGTT AGCTTCCTCT TACCGGATAT CACTGACACA 
TTTGAAGCCT TCACCTTGAG CCTTCTGTCC TCCCTCCTGA TTGCTGGACC CAACTCCCCC 
TTCTACAAAG CTTTGATCGA GTCTGGACTC GGCACAGACT TTTCTCCTGA TGTTGGATAT 
AATGGCTATA CACGGGAGGC TTACTTCAGT GTCGGGCTCC AAGGGATCGC AGAGAAAGAT 
GTCAAGACGG TCAGAGAGCT CGTAGACAGG ACAATCGAAG AAGTTATAGA GAAAGGATTT 
GAAGATGATC GGATTGAAGC TCTGCTTCAT AAAATCGAAA TTCAAACGAA GCATCAGTCA 
GCCAGCTTTG GCCTGACCCT GACGTCATAT ATAGCTTCTT GCTGGAACCA TGATGGGGAC 
CCTGTGGAGC TCCTGCAGAT TGGAAGTCAG CTGACTAGAT TTAGGAAGTG CCTTAAGGAA 
AATCCAAAAT TTTTACAAGA AAAAGTAGAA CAATATTTTA AGAACAATCA GCACAAGCTG 
ACTTTATCCA TGAAGCCAGA CGACAAGTAT TATGAAAAGC AAACTCAGAT GGAGACAGAA 
AAGCTGGAGC AAAAGGTGAA TTCTCTCTCC CCGGCGGACA AGCAGCAGAT CTACGAGAAA 
GGTTTAGAAC TACAGACGCA GCAAAGTAAA CATCAAGACG CCTCCTGCCT CCCAGCATTG 
AAAGTCTCGG ACATTGAGCC CTCCATGCCT TTCACCAAGC TTGACATCGG CCTTGCAGCT 
GGAGACATCC CTGTGCAGTA CTGCCCACAG CCCACCAACG GCATGGTGTA TTTCCGAGCC 
TTTTCCAGTT TAAACACGCT GCCGGAGGAC CTGAGGCCCA TTGTGCCTCT CTTTTGCAGC 
GTGCTGACCA AGCTGGGTTG TGGCATCCTT AACTACAGAG AGCAAGCCCA ACAGATTGAG 
CTCAAGACAG GAGGCATGAG TGTCACGCCC CATGTGCTCC CTGACGACTC ACAGCTGGAT 
ACCTACGAGC AGGGTGTGTT ATTTTCATCT CTCTGCCTGG AGCGGAACCT GCCAGACATG 
ATGCATCTGT GGAGCGAAAT ATTTAACAAT CCATGCTTTG AAGAAGAAGA ACACTTCAAA 
GTGTTGGTGA AGATGACCGC TCAGGAGCTC TCCAATGGAA TTTCAGACTC GGGGCATCTC 
TATGCAGCCC TCAGAGCAAG CAAGACACTG ACACCTTCAG GGGACTTGCA GGAGACCTTC 
AGTGGGATGG ATCAGGTGAA GGTGATGAAA AGAATTGCAG AGATGACAGA CATCAAGCCC 
ATCCTGAGAA AACTGCCCCG GATCAAGAAG TATCTACTAA ACTGTGACAA CATGAGATGC 
TCAGTGAATG CCACCCCTCA GCAGATGCCT CAGGCAGAAA AAGAGGTGGA AAACTTCCTT 
AGAAATGTTG GCCGAAGCAA AAAGGAACGG AAGCCTGTCC GCCCGCATAT TGTCGAGAAA 
CCCACACCCA GTGGCCCCAG TGGAGCTGCA CATGTCAGTG GGTCCCAGAT CGTCAGAAAA 
TTGGTGACAT GTCCTCGTAC GTCAAGTTCT GCTCTCAGGA GTGCCTCCCT AGTGACACCC 
ATGGAGAAGG AGATGCTGGA GTGGTGCCGC CTGCTTTTAC TACCTGAGGG CCACCTTGGG 
CTGTACATCC TAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516572.1
CDS	53..2710
Translation	MWRFSGRRGLCAVQRLSCGRVHHRVWREKSDQACERALQYKVGEKIHGFTVNQVTPVPELFLTAVKLSHDNTGARYLHLAREDKNNLFSVQFRTTPMDSTGVPHVLEHTVLCGSQKYPCRDPFFKMLNRSLSTFMNAMTASDYTIYPFSTQNPKDFQNLLSVYLDATFFPCLRELDFWQEGWRLEHENPRDPQTPLIFKGVVFNEMKGAFTDNERIFSQHLQNKLLPDHTYSVVSGGDPLCIPELTWEQLKQFHATHYHPSNARFFTYGNFQLEGHLKQIHEEALSKFQRLEQSTAVPAQPHWDKPREFHITCGPDSLATETAKQTTVSVSFLLPDITDTFEAFTLSLLSSLLIAGPNSPFYKALIESGLGTDFSPDVGYNGYTREAYFSVGLQGIAEKDVKTVRELVDRTIEEVIEKGFEDDRIEALLHKIEIQTKHQSASFGLTLTSYIASCWNHDGDPVELLQIGSQLTRFRKCLKENPKFLQEKVEQYFKNNQHKLTLSMKPDDKYYEKQTQMETEKLEQKVNSLSPADKQQIYEKGLELQTQQSKHQDASCLPALKVSDIEPSMPFTKLDIGLAAGDIPVQYCPQPTNGMVYFRAFSSLNTLPEDLRPIVPLFCSVLTKLGCGILNYREQAQQIELKTGGMSVTPHVLPDDSQLDTYEQGVLFSSLCLERNLPDMMHLWSEIFNNPCFEEEEHFKVLVKMTAQELSNGISDSGHLYAALRASKTLTPSGDLQETFSGMDQVKVMKRIAEMTDIKPILRKLPRIKKYLLNCDNMRCSVNATPQQMPQAEKEVENFLRNVGRSKKERKPVRPHIVEKPTPSGPSGAAHVSGSQIVRKLVTCPRTSSSALRSASLVTPMEKEMLEWCRLLLLPEGHLGLYILV

Target ORF information:

RefSeq Version	XM_006516509.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus pitrilysin metallepetidase 1 (Pitrm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516509.3

ORF Insert Sequence:

ATGTGGCGCT TCAGCGGTCG GCGGGGACTC TGCGCTGTAC AGCGGCTGAG CTGCGGCAGG 
GTACACCACA GAGTATGGAG GGAGAAGAGT GACCAAGCCT GTGAACGAGC TCTACAGTAT 
AAAGTGGGAG AGAAAATCCA CGGGTTCACT GTAAACCAGG TCACTCCTGT CCCCGAGCTG 
TTCCTGACAG CCGTGAAGCT CAGCCATGAC AACACGGGAG CCAGATACCT GCACCTGGCA 
AGGGAAGACA AGAACAACTT ATTCAGTGTG CAGTTCCGCA CAACCCCAAT GGATAGCACT 
GGGGTCCCAC ATGTTCTCGA GCATACGGTC CTGTGCGGCT CTCAGAAGTA CCCGTGCAGA 
GATCCTTTCT TCAAAATGCT CAACAGGTCA CTGTCCACAT TTATGAATGC CATGACAGCC 
AGCGATTACA CGATATATCC GTTTTCCACT CAAAATCCCA AAGATTTTCA GAACCTCCTC 
TCCGTGTATT TGGATGCAAC TTTCTTCCCC TGCTTGAGGG AACTGGACTT CTGGCAGGAA 
GGATGGCGTC TGGAGCATGA GAATCCCCGA GACCCTCAGA CGCCCTTGAT CTTTAAGGGG 
GTCGTCTTCA ACGAGATGAA AGGGGCATTT ACAGACAATG AGAGGATATT CTCCCAGCAC 
CTGCAGAACA AGCTGCTTCC TGACCACACC TACTCCGTGG TTTCTGGAGG GGACCCACTG 
TGCATCCCGG AGCTCACGTG GGAACAGCTG AAACAGTTCC ACGCTACTCA TTATCACCCA 
AGCAATGCCA GGTTCTTCAC TTATGGCAAT TTTCAGCTGG AAGGACACCT GAAACAAATT 
CACGAAGAAG CCCTGAGTAA ATTCCAGAGA TTGGAGCAGA GTACAGCAGT GCCTGCCCAG 
CCGCACTGGG ATAAGCCTAG GGAATTCCAT ATAACATGTG GCCCAGATTC ACTAGCTACG 
GAGACTGCCA AGCAGACAAC TGTCAGCGTT AGCTTCCTCT TACCGGATAT CACTGACACA 
TTTGAAGCCT TCACCTTGAG CCTTCTGTCC TCCCTCCTGA TTGCTGGACC CAACTCCCCC 
TTCTACAAAG CTTTGATCGA GTCTGGACTC GGCACAGACT TTTCTCCTGA TGTTGGATAT 
AATGGCTATA CACGGGAGGC TTACTTCAGT GTCGGGCTCC AAGGGATCGC AGAGAAAGAT 
GTCAAGACGG TCAGAGAGCT CGTAGACAGG ACAATCGAAG AAGTTATAGA GAAAGGATTT 
GAAGATGATC GGATTGAAGC TCTGCTTCAT AAAATCGAAA TTCAAACGAA GCATCAGTCA 
GCCAGCTTTG GCCTGACCCT GACGTCATAT ATAGCTTCTT GCTGGAACCA TGATGGGGAC 
CCTGTGGAGC TCCTGCAGAT TGGAAGTCAG CTGACTAGAT TTAGGAAGTG CCTTAAGGAA 
AATCCAAAAT TTTTACAAGA AAAAGTAGAA CAATATTTTA AGAACAATCA GCACAAGCTG 
ACTTTATCCA TGAAGCCAGA CGACAAGTAT TATGAAAAGC AAACTCAGAT GGAGACAGAA 
AAGCTGGAGC AAAAGGTGAA TTCTCTCTCC CCGGCGGACA AGCAGCAGAT CTACGAGAAA 
GGTTTAGAAC TACAGACGCA GCAAAGTAAA CATCAAGACG CCTCCTGCCT CCCAGCATTG 
AAAGTCTCGG ACATTGAGCC CTCCATGCCT TTCACCAAGC TTGACATCGG CCTTGCAGCT 
GGAGACATCC CTGTGCAGTA CTGCCCACAG CCCACCAACG GCATGGTGTA TTTCCGAGCC 
TTTTCCAGTT TAAACACGCT GCCGGAGGAC CTGAGGCCCA TTGTGCCTCT CTTTTGCAGC 
GTGCTGACCA AGCTGGGTTG TGGCATCCTT AACTACAGAG AGCAAGCCCA ACAGATTGAG 
CTCAAGACAG GAGGCATGAG TGTCACGCCC CATGTGCTCC CTGACGACTC ACAGCTGGAT 
ACCTACGAGC AGGGTGTGTT ATTTTCATCT CTCTGCCTGG AGCGGAACCT GCCAGACATG 
ATGCATCTGT GGAGCGAAAT ATTTAACAAT CCATGCTTTG AAGAAGAAGA ACACTTCAAA 
GTGTTGGTGA AGATGACCGC TCAGGAGCTC TCCAATGGAA TTTCAGACTC GGGGCATCTC 
TATGCAGCCC TCAGAGCAAG CAAGACACTG ACACCTTCAG GGGACTTGCA GGAGACCTTC 
AGTGGGATGG ATCAGGTGAA GGTGATGAAA AGAATTGCAG AGATGACAGA CATCAAGCCC 
ATCCTGAGAA AACTGCCCCG GATCAAGAAG TATCTACTAA ACTGTGACAA CATGAGATGC 
TCAGTGAATG CCACCCCTCA GCAGATGCCT CAGGCAGAAA AAGAGGTGGA AAACTTCCTT 
AGAAATGTTG GCCGAAGCAA AAAGGAACGG AAGCCTGTCC GCCCGCATAT TGTCGAGAAA 
CCCACACCCA GTGGCCCCAG TGGAGCTGCA CATGTCAGTG GGTCCCAGAT CGTCAGAAAA 
TTGGTGACAT GTCCTCGTAC GTCAAGTTCT GCTCTCAGGA GTGCCTCCCT AGTGACACCC 
ATGGAGAAGG AGATGCTGGA GTGGTGCCGC CTGCTTTTAC TACCTGAGGG CCACCTTGGG 
CTGTACATCC TAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pitrm1 ( NM_145131.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_660113.1 Mus musculus pitrilysin metallepetidase 1 (Pitrm1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14511
Clone ID Related Accession (Same CDS sequence)	NM_145131.1
Accession Version	NM_145131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571414400000
Organism	Mus musculus(house mouse)
Product	presequence protease, mitochondrial isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF513714.1. ##Evidence-Data-START## Transcript exon combination :: AF513714.1, AK141277.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTGGCGCT TCAGCGGTCG GCGGGGACTC TGCGCTGTAC AGCGGCTGAG CTGCGGCAGG 
GTACACCACA GAGTATGGAG GGAGAAGAGT GACCAAGCCT GTGAACGAGC TCTACAGTAT 
AAAGTGGGAG AGAAAATCCA CGGGTTCACT GTAAACCAGG TCACTCCTGT CCCCGAGCTG 
TTCCTGACAG CCGTGAAGCT CAGCCATGAC AACACGGGAG CCAGATACCT GCACCTGGCA 
AGGGAAGACA AGAACAACTT ATTCAGTGTG CAGTTCCGCA CAACCCCAAT GGATAGCACT 
GGGGTCCCAC ATGTTCTCGA GCATACGGTC CTGTGCGGCT CTCAGAAGTA CCCGTGCAGA 
GATCCTTTCT TCAAAATGCT CAACAGGTCA CTGTCCACAT TTATGAATGC CATGACAGCC 
AGCGATTACA CGATATATCC GTTTTCCACT CAAAATCCCA AAGATTTTCA GAACCTCCTC 
TCCGTGTATT TGGATGCAAC TTTCTTCCCC TGCTTGAGGG AACTGGACTT CTGGCAGGAA 
GGATGGCGTC TGGAGCATGA GAATCCCCGA GACCCTCAGA CGCCCTTGAT CTTTAAGGGG 
GTCGTCTTCA ACGAGATGAA AGGGGCATTT ACAGACAATG AGAGGATATT CTCCCAGCAC 
CTGCAGAACA AGCTGCTTCC TGACCACACC TACTCCGTGG TTTCTGGAGG GGACCCACTG 
TGCATCCCGG AGCTCACGTG GGAACAGCTG AAACAGTTCC ACGCTACTCA TTATCACCCA 
AGCAATGCCA GGTTCTTCAC TTATGGCAAT TTTCAGCTGG AAGGACACCT GAAACAAATT 
CACGAAGAAG CCCTGAGTAA ATTCCAGAGA TTGGAGCAGA GTACAGCAGT GCCTGCCCAG 
CCGCACTGGG ATAAGCCTAG GGAATTCCAT ATAACATGTG GCCCAGATTC ACTAGCTACG 
GAGACTGCCA AGCAGACAAC TGTCAGCGTT AGCTTCCTCT TACCGGATAT CACTGACACA 
TTTGAAGCCT TCACCTTGAG CCTTCTGTCC TCCCTCCTGA TTGCTGGACC CAACTCCCCC 
TTCTACAAAG CTTTGATCGA GTCTGGACTC GGCACAGACT TTTCTCCTGA TGTTGGATAT 
AATGGCTATA CACGGGAGGC TTACTTCAGT GTCGGGCTCC AAGGGATCGC AGAGAAAGAT 
GTCAAGACGG TCAGAGAGCT CGTAGACAGG ACAATCGAAG AAGTTATAGA GAAAGGATTT 
GAAGATGATC GGATTGAAGC TCTGCTTCAT AAAATCGAAA TTCAAACGAA GCATCAGTCA 
GCCAGCTTTG GCCTGACCCT GACGTCATAT ATAGCTTCTT GCTGGAACCA TGATGGGGAC 
CCTGTGGAGC TCCTGCAGAT TGGAAGTCAG CTGACTAGAT TTAGGAAGTG CCTTAAGGAA 
AATCCAAAAT TTTTACAAGA AAAAGTAGAA CAATATTTTA AGAACAATCA GCACAAGCTG 
ACTTTATCCA TGAAGCCAGA CGACAAGTAT TATGAAAAGC AAACTCAGAT GGAGACAGAA 
AAGCTGGAGC AAAAGGTGAA TTCTCTCTCC CCGGCGGACA AGCAGCAGAT CTACGAGAAA 
GGTTTAGAAC TACAGACGCA GCAAAGTAAA CATCAAGACG CCTCCTGCCT CCCAGCATTG 
AAAGTCTCGG ACATTGAGCC CTCCATGCCT TTCACCAAGC TTGACATCGG CCTTGCAGCT 
GGAGACATCC CTGTGCAGTA CTGCCCACAG CCCACCAACG GCATGGTGTA TTTCCGAGCC 
TTTTCCAGTT TAAACACGCT GCCGGAGGAC CTGAGGCCCA TTGTGCCTCT CTTTTGCAGC 
GTGCTGACCA AGCTGGGTTG TGGCATCCTT AACTACAGAG AGCAAGCCCA ACAGATTGAG 
CTCAAGACAG GAGGCATGAG TGTCACGCCC CATGTGCTCC CTGACGACTC ACAGCTGGAT 
ACCTACGAGC AGGGTGTGTT ATTTTCATCT CTCTGCCTGG AGCGGAACCT GCCAGACATG 
ATGCATCTGT GGAGCGAAAT ATTTAACAAT CCATGCTTTG AAGAAGAAGA ACACTTCAAA 
GTGTTGGTGA AGATGACCGC TCAGGAGCTC TCCAATGGAA TTTCAGACTC GGGGCATCTC 
TATGCAGCCC TCAGAGCAAG CAAGACACTG ACACCTTCAG GGGACTTGCA GGAGACCTTC 
AGTGGGATGG ATCAGGTGAA GGTGATGAAA AGAATTGCAG AGATGACAGA CATCAAGCCC 
ATCCTGAGAA AACTGCCCCG GATCAAGAAG TATCTACTAA ACTGTGACAA CATGAGATGC 
TCAGTGAATG CCACCCCTCA GCAGATGCCT CAGGCAGAAA AAGAGGTGGA AAACTTCCTT 
AGAAATGTTG GCCGAAGCAA AAAGGAACGG AAGCCTGTCC GCCCGCATAT TGTCGAGAAA 
CCCACACCCA GTGGCCCCAG TGGAGCTGCA CATGTCAGTG GGTCCCAGAT CGTCAGAAAA 
TTGGTGACAG ACCCCACCTT CAAACCCTGC CAGATGAAGA CACATTTTGT GCTGCCCTTC 
CCTGTGAATT ACATTGGCGA GTGTGTCAGG ACTGTCCCGT ATGCTGATCC AGACCATGCC 
AGCCTTAAGA TCCTTGCCCG TCTAATGACA GCTAAATTCT TGCATACGGA AATTCGAGAG 
AAGGGGGGTG CTTATGGTGG CGGTGCTAAA CTCACCCACA GTGGGATTTT CACGCTTTAC 
TCTTACAGGG ATCCCAATTC CATAGAAACA CTCCAGTCTT TTGGGAAAGC TGTAGACTGG 
GCTAAGTCTG GAAAGTTCAC ACAGCAGGAC ATTGATGAAG CCAAGCTGTC TGTTTTCTCT 
ACTGTGGATT CTCCTGTTGC TCCATCCGAT AAAGGAATGG ACCACTTCTT GTATGGCCTC 
TCCGATGAGA TGAAGCAGGC ATACCGAGAA CAGCTCTTTG CTGTCAACCA CGACAAACTG 
ACCTCTGTGA GCCATAAATA CCTTGGCATC GGGAAGAGCA CACACGGCCT GGCTATCCTC 
GGACCAGAGA ACTCAAAAAT TGCCAAAGAC CCATCATGGA TCATAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660113.1
CDS	18..3128
Translation	MWRFSGRRGLCAVQRLSCGRVHHRVWREKSDQACERALQYKVGEKIHGFTVNQVTPVPELFLTAVKLSHDNTGARYLHLAREDKNNLFSVQFRTTPMDSTGVPHVLEHTVLCGSQKYPCRDPFFKMLNRSLSTFMNAMTASDYTIYPFSTQNPKDFQNLLSVYLDATFFPCLRELDFWQEGWRLEHENPRDPQTPLIFKGVVFNEMKGAFTDNERIFSQHLQNKLLPDHTYSVVSGGDPLCIPELTWEQLKQFHATHYHPSNARFFTYGNFQLEGHLKQIHEEALSKFQRLEQSTAVPAQPHWDKPREFHITCGPDSLATETAKQTTVSVSFLLPDITDTFEAFTLSLLSSLLIAGPNSPFYKALIESGLGTDFSPDVGYNGYTREAYFSVGLQGIAEKDVKTVRELVDRTIEEVIEKGFEDDRIEALLHKIEIQTKHQSASFGLTLTSYIASCWNHDGDPVELLQIGSQLTRFRKCLKENPKFLQEKVEQYFKNNQHKLTLSMKPDDKYYEKQTQMETEKLEQKVNSLSPADKQQIYEKGLELQTQQSKHQDASCLPALKVSDIEPSMPFTKLDIGLAAGDIPVQYCPQPTNGMVYFRAFSSLNTLPEDLRPIVPLFCSVLTKLGCGILNYREQAQQIELKTGGMSVTPHVLPDDSQLDTYEQGVLFSSLCLERNLPDMMHLWSEIFNNPCFEEEEHFKVLVKMTAQELSNGISDSGHLYAALRASKTLTPSGDLQETFSGMDQVKVMKRIAEMTDIKPILRKLPRIKKYLLNCDNMRCSVNATPQQMPQAEKEVENFLRNVGRSKKERKPVRPHIVEKPTPSGPSGAAHVSGSQIVRKLVTDPTFKPCQMKTHFVLPFPVNYIGECVRTVPYADPDHASLKILARLMTAKFLHTEIREKGGAYGGGAKLTHSGIFTLYSYRDPNSIETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSTVDSPVAPSDKGMDHFLYGLSDEMKQAYREQLFAVNHDKLTSVSHKYLGIGKSTHGLAILGPENSKIAKDPSWIIK

Target ORF information:

RefSeq Version	NM_145131.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pitrilysin metallepetidase 1 (Pitrm1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145131.1

ORF Insert Sequence:

ATGTGGCGCT TCAGCGGTCG GCGGGGACTC TGCGCTGTAC AGCGGCTGAG CTGCGGCAGG 
GTACACCACA GAGTATGGAG GGAGAAGAGT GACCAAGCCT GTGAACGAGC TCTACAGTAT 
AAAGTGGGAG AGAAAATCCA CGGGTTCACT GTAAACCAGG TCACTCCTGT CCCCGAGCTG 
TTCCTGACAG CCGTGAAGCT CAGCCATGAC AACACGGGAG CCAGATACCT GCACCTGGCA 
AGGGAAGACA AGAACAACTT ATTCAGTGTG CAGTTCCGCA CAACCCCAAT GGATAGCACT 
GGGGTCCCAC ATGTTCTCGA GCATACGGTC CTGTGCGGCT CTCAGAAGTA CCCGTGCAGA 
GATCCTTTCT TCAAAATGCT CAACAGGTCA CTGTCCACAT TTATGAATGC CATGACAGCC 
AGCGATTACA CGATATATCC GTTTTCCACT CAAAATCCCA AAGATTTTCA GAACCTCCTC 
TCCGTGTATT TGGATGCAAC TTTCTTCCCC TGCTTGAGGG AACTGGACTT CTGGCAGGAA 
GGATGGCGTC TGGAGCATGA GAATCCCCGA GACCCTCAGA CGCCCTTGAT CTTTAAGGGG 
GTCGTCTTCA ACGAGATGAA AGGGGCATTT ACAGACAATG AGAGGATATT CTCCCAGCAC 
CTGCAGAACA AGCTGCTTCC TGACCACACC TACTCCGTGG TTTCTGGAGG GGACCCACTG 
TGCATCCCGG AGCTCACGTG GGAACAGCTG AAACAGTTCC ACGCTACTCA TTATCACCCA 
AGCAATGCCA GGTTCTTCAC TTATGGCAAT TTTCAGCTGG AAGGACACCT GAAACAAATT 
CACGAAGAAG CCCTGAGTAA ATTCCAGAGA TTGGAGCAGA GTACAGCAGT GCCTGCCCAG 
CCGCACTGGG ATAAGCCTAG GGAATTCCAT ATAACATGTG GCCCAGATTC ACTAGCTACG 
GAGACTGCCA AGCAGACAAC TGTCAGCGTT AGCTTCCTCT TACCGGATAT CACTGACACA 
TTTGAAGCCT TCACCTTGAG CCTTCTGTCC TCCCTCCTGA TTGCTGGACC CAACTCCCCC 
TTCTACAAAG CTTTGATCGA GTCTGGACTC GGCACAGACT TTTCTCCTGA TGTTGGATAT 
AATGGCTATA CACGGGAGGC TTACTTCAGT GTCGGGCTCC AAGGGATCGC AGAGAAAGAT 
GTCAAGACGG TCAGAGAGCT CGTAGACAGG ACAATCGAAG AAGTTATAGA GAAAGGATTT 
GAAGATGATC GGATTGAAGC TCTGCTTCAT AAAATCGAAA TTCAAACGAA GCATCAGTCA 
GCCAGCTTTG GCCTGACCCT GACGTCATAT ATAGCTTCTT GCTGGAACCA TGATGGGGAC 
CCTGTGGAGC TCCTGCAGAT TGGAAGTCAG CTGACTAGAT TTAGGAAGTG CCTTAAGGAA 
AATCCAAAAT TTTTACAAGA AAAAGTAGAA CAATATTTTA AGAACAATCA GCACAAGCTG 
ACTTTATCCA TGAAGCCAGA CGACAAGTAT TATGAAAAGC AAACTCAGAT GGAGACAGAA 
AAGCTGGAGC AAAAGGTGAA TTCTCTCTCC CCGGCGGACA AGCAGCAGAT CTACGAGAAA 
GGTTTAGAAC TACAGACGCA GCAAAGTAAA CATCAAGACG CCTCCTGCCT CCCAGCATTG 
AAAGTCTCGG ACATTGAGCC CTCCATGCCT TTCACCAAGC TTGACATCGG CCTTGCAGCT 
GGAGACATCC CTGTGCAGTA CTGCCCACAG CCCACCAACG GCATGGTGTA TTTCCGAGCC 
TTTTCCAGTT TAAACACGCT GCCGGAGGAC CTGAGGCCCA TTGTGCCTCT CTTTTGCAGC 
GTGCTGACCA AGCTGGGTTG TGGCATCCTT AACTACAGAG AGCAAGCCCA ACAGATTGAG 
CTCAAGACAG GAGGCATGAG TGTCACGCCC CATGTGCTCC CTGACGACTC ACAGCTGGAT 
ACCTACGAGC AGGGTGTGTT ATTTTCATCT CTCTGCCTGG AGCGGAACCT GCCAGACATG 
ATGCATCTGT GGAGCGAAAT ATTTAACAAT CCATGCTTTG AAGAAGAAGA ACACTTCAAA 
GTGTTGGTGA AGATGACCGC TCAGGAGCTC TCCAATGGAA TTTCAGACTC GGGGCATCTC 
TATGCAGCCC TCAGAGCAAG CAAGACACTG ACACCTTCAG GGGACTTGCA GGAGACCTTC 
AGTGGGATGG ATCAGGTGAA GGTGATGAAA AGAATTGCAG AGATGACAGA CATCAAGCCC 
ATCCTGAGAA AACTGCCCCG GATCAAGAAG TATCTACTAA ACTGTGACAA CATGAGATGC 
TCAGTGAATG CCACCCCTCA GCAGATGCCT CAGGCAGAAA AAGAGGTGGA AAACTTCCTT 
AGAAATGTTG GCCGAAGCAA AAAGGAACGG AAGCCTGTCC GCCCGCATAT TGTCGAGAAA 
CCCACACCCA GTGGCCCCAG TGGAGCTGCA CATGTCAGTG GGTCCCAGAT CGTCAGAAAA 
TTGGTGACAG ACCCCACCTT CAAACCCTGC CAGATGAAGA CACATTTTGT GCTGCCCTTC 
CCTGTGAATT ACATTGGCGA GTGTGTCAGG ACTGTCCCGT ATGCTGATCC AGACCATGCC 
AGCCTTAAGA TCCTTGCCCG TCTAATGACA GCTAAATTCT TGCATACGGA AATTCGAGAG 
AAGGGGGGTG CTTATGGTGG CGGTGCTAAA CTCACCCACA GTGGGATTTT CACGCTTTAC 
TCTTACAGGG ATCCCAATTC CATAGAAACA CTCCAGTCTT TTGGGAAAGC TGTAGACTGG 
GCTAAGTCTG GAAAGTTCAC ACAGCAGGAC ATTGATGAAG CCAAGCTGTC TGTTTTCTCT 
ACTGTGGATT CTCCTGTTGC TCCATCCGAT AAAGGAATGG ACCACTTCTT GTATGGCCTC 
TCCGATGAGA TGAAGCAGGC ATACCGAGAA CAGCTCTTTG CTGTCAACCA CGACAAACTG 
ACCTCTGTGA GCCATAAATA CCTTGGCATC GGGAAGAGCA CACACGGCCT GGCTATCCTC 
GGACCAGAGA ACTCAAAAAT TGCCAAAGAC CCATCATGGA TCATAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pitrm1 ( NM_001360106.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347035.1 Mus musculus pitrilysin metallepetidase 1 (Pitrm1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu48299
Clone ID Related Accession (Same CDS sequence)	NM_001360106.1 , XM_006516508.1
Accession Version	NM_001360106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571587200000
Organism	Mus musculus(house mouse)
Product	presequence protease, mitochondrial isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC159228.2. On Jan 27, 2018 this sequence version replaced XM_006516508.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK164235.1, AK167426.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTGGCGCT TCAGCGGTCG GCGGGGACTC TGCGCTGTAC AGCGGCTGAG CTGCGGGGTA 
CACCACAGAG TATGGAGGGA GAAGAGTGAC CAAGCCTGTG AACGAGCTCT ACAGTATAAA 
GTGGGAGAGA AAATCCACGG GTTCACTGTA AACCAGGTCA CTCCTGTCCC CGAGCTGTTC 
CTGACAGCCG TGAAGCTCAG CCATGACAAC ACGGGAGCCA GATACCTGCA CCTGGCAAGG 
GAAGACAAGA ACAACTTATT CAGTGTGCAG TTCCGCACAA CCCCAATGGA TAGCACTGGG 
GTCCCACATG TTCTCGAGCA TACGGTCCTG TGCGGCTCTC AGAAGTACCC GTGCAGAGAT 
CCTTTCTTCA AAATGCTCAA CAGGTCACTG TCCACATTTA TGAATGCCAT GACAGCCAGC 
GATTACACGA TATATCCGTT TTCCACTCAA AATCCCAAAG ATTTTCAGAA CCTCCTCTCC 
GTGTATTTGG ATGCAACTTT CTTCCCCTGC TTGAGGGAAC TGGACTTCTG GCAGGAAGGA 
TGGCGTCTGG AGCATGAGAA TCCCCGAGAC CCTCAGACGC CCTTGATCTT TAAGGGGGTC 
GTCTTCAACG AGATGAAAGG GGCATTTACA GACAATGAGA GGATATTCTC CCAGCACCTG 
CAGAACAAGC TGCTTCCTGA CCACACCTAC TCCGTGGTTT CTGGAGGGGA CCCACTGTGC 
ATCCCGGAGC TCACGTGGGA ACAGCTGAAA CAGTTCCACG CTACTCATTA TCACCCAAGC 
AATGCCAGGT TCTTCACTTA TGGCAATTTT CAGCTGGAAG GACACCTGAA ACAAATTCAC 
GAAGAAGCCC TGAGTAAATT CCAGAGATTG GAGCAGAGTA CAGCAGTGCC TGCCCAGCCG 
CACTGGGATA AGCCTAGGGA ATTCCATATA ACATGTGGCC CAGATTCACT AGCTACGGAG 
ACTGCCAAGC AGACAACTGT CAGCGTTAGC TTCCTCTTAC CGGATATCAC TGACACATTT 
GAAGCCTTCA CCTTGAGCCT TCTGTCCTCC CTCCTGATTG CTGGACCCAA CTCCCCCTTC 
TACAAAGCTT TGATCGAGTC TGGACTCGGC ACAGACTTTT CTCCTGATGT TGGATATAAT 
GGCTATACAC GGGAGGCTTA CTTCAGTGTC GGGCTCCAAG GGATCGCAGA GAAAGATGTC 
AAGACGGTCA GAGAGCTCGT AGACAGGACA ATCGAAGAAG TTATAGAGAA AGGATTTGAA 
GATGATCGGA TTGAAGCTCT GCTTCATAAA ATCGAAATTC AAACGAAGCA TCAGTCAGCC 
AGCTTTGGCC TGACCCTGAC GTCATATATA GCTTCTTGCT GGAACCATGA TGGGGACCCT 
GTGGAGCTCC TGCAGATTGG AAGTCAGCTG ACTAGATTTA GGAAGTGCCT TAAGGAAAAT 
CCAAAATTTT TACAAGAAAA AGTAGAACAA TATTTTAAGA ACAATCAGCA CAAGCTGACT 
TTATCCATGA AGCCAGACGA CAAGTATTAT GAAAAGCAAA CTCAGATGGA GACAGAAAAG 
CTGGAGCAAA AGGTGAATTC TCTCTCCCCG GCGGACAAGC AGCAGATCTA CGAGAAAGGT 
TTAGAACTAC AGACGCAGCA AAGTAAACAT CAAGACGCCT CCTGCCTCCC AGCATTGAAA 
GTCTCGGACA TTGAGCCCTC CATGCCTTTC ACCAAGCTTG ACATCGGCCT TGCAGCTGGA 
GACATCCCTG TGCAGTACTG CCCACAGCCC ACCAACGGCA TGGTGTATTT CCGAGCCTTT 
TCCAGTTTAA ACACGCTGCC GGAGGACCTG AGGCCCATTG TGCCTCTCTT TTGCAGCGTG 
CTGACCAAGC TGGGTTGTGG CATCCTTAAC TACAGAGAGC AAGCCCAACA GATTGAGCTC 
AAGACAGGAG GCATGAGTGT CACGCCCCAT GTGCTCCCTG ACGACTCACA GCTGGATACC 
TACGAGCAGG GTGTGTTATT TTCATCTCTC TGCCTGGAGC GGAACCTGCC AGACATGATG 
CATCTGTGGA GCGAAATATT TAACAATCCA TGCTTTGAAG AAGAAGAACA CTTCAAAGTG 
TTGGTGAAGA TGACCGCTCA GGAGCTCTCC AATGGAATTT CAGACTCGGG GCATCTCTAT 
GCAGCCCTCA GAGCAAGCAA GACACTGACA CCTTCAGGGG ACTTGCAGGA GACCTTCAGT 
GGGATGGATC AGGTGAAGGT GATGAAAAGA ATTGCAGAGA TGACAGACAT CAAGCCCATC 
CTGAGAAAAC TGCCCCGGAT CAAGAAGTAT CTACTAAACT GTGACAACAT GAGATGCTCA 
GTGAATGCCA CCCCTCAGCA GATGCCTCAG GCAGAAAAAG AGGTGGAAAA CTTCCTTAGA 
AATGTTGGCC GAAGCAAAAA GGAACGGAAG CCTGTCCGCC CGCATATTGT CGAGAAACCC 
ACACCCAGTG GCCCCAGTGG AGCTGCACAT GTCAGTGGGT CCCAGATCGT CAGAAAATTG 
GTGACAGACC CCACCTTCAA ACCCTGCCAG ATGAAGACAC ATTTTGTGCT GCCCTTCCCT 
GTGAATTACA TTGGCGAGTG TGTCAGGACT GTCCCGTATG CTGATCCAGA CCATGCCAGC 
CTTAAGATCC TTGCCCGTCT AATGACAGCT AAATTCTTGC ATACGGAAAT TCGAGAGAAG 
GGGGGTGCTT ATGGTGGCGG TGCTAAACTC ACCCACAGTG GGATTTTCAC GCTTTACTCT 
TACAGGGATC CCAATTCCAT AGAAACACTC CAGTCTTTTG GGAAAGCTGT AGACTGGGCT 
AAGTCTGGAA AGTTCACACA GCAGGACATT GATGAAGCCA AGCTGTCTGT TTTCTCTACT 
GTGGATTCTC CTGTTGCTCC ATCCGATAAA GGAATGGACC ACTTCTTGTA TGGCCTCTCC 
GATGAGATGA AGCAGGCATA CCGAGAACAG CTCTTTGCTG TCAACCACGA CAAACTGACC 
TCTGTGAGCC ATAAATACCT TGGCATCGGG AAGAGCACAC ACGGCCTGGC TATCCTCGGA 
CCAGAGAACT CAAAAATTGC CAAAGACCCA TCATGGATCA TAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347035.1
CDS	18..3125
Translation	MWRFSGRRGLCAVQRLSCGVHHRVWREKSDQACERALQYKVGEKIHGFTVNQVTPVPELFLTAVKLSHDNTGARYLHLAREDKNNLFSVQFRTTPMDSTGVPHVLEHTVLCGSQKYPCRDPFFKMLNRSLSTFMNAMTASDYTIYPFSTQNPKDFQNLLSVYLDATFFPCLRELDFWQEGWRLEHENPRDPQTPLIFKGVVFNEMKGAFTDNERIFSQHLQNKLLPDHTYSVVSGGDPLCIPELTWEQLKQFHATHYHPSNARFFTYGNFQLEGHLKQIHEEALSKFQRLEQSTAVPAQPHWDKPREFHITCGPDSLATETAKQTTVSVSFLLPDITDTFEAFTLSLLSSLLIAGPNSPFYKALIESGLGTDFSPDVGYNGYTREAYFSVGLQGIAEKDVKTVRELVDRTIEEVIEKGFEDDRIEALLHKIEIQTKHQSASFGLTLTSYIASCWNHDGDPVELLQIGSQLTRFRKCLKENPKFLQEKVEQYFKNNQHKLTLSMKPDDKYYEKQTQMETEKLEQKVNSLSPADKQQIYEKGLELQTQQSKHQDASCLPALKVSDIEPSMPFTKLDIGLAAGDIPVQYCPQPTNGMVYFRAFSSLNTLPEDLRPIVPLFCSVLTKLGCGILNYREQAQQIELKTGGMSVTPHVLPDDSQLDTYEQGVLFSSLCLERNLPDMMHLWSEIFNNPCFEEEEHFKVLVKMTAQELSNGISDSGHLYAALRASKTLTPSGDLQETFSGMDQVKVMKRIAEMTDIKPILRKLPRIKKYLLNCDNMRCSVNATPQQMPQAEKEVENFLRNVGRSKKERKPVRPHIVEKPTPSGPSGAAHVSGSQIVRKLVTDPTFKPCQMKTHFVLPFPVNYIGECVRTVPYADPDHASLKILARLMTAKFLHTEIREKGGAYGGGAKLTHSGIFTLYSYRDPNSIETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSTVDSPVAPSDKGMDHFLYGLSDEMKQAYREQLFAVNHDKLTSVSHKYLGIGKSTHGLAILGPENSKIAKDPSWIIK

Target ORF information:

RefSeq Version	NM_001360106.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pitrilysin metallepetidase 1 (Pitrm1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360106.1

ORF Insert Sequence:

ATGTGGCGCT TCAGCGGTCG GCGGGGACTC TGCGCTGTAC AGCGGCTGAG CTGCGGGGTA 
CACCACAGAG TATGGAGGGA GAAGAGTGAC CAAGCCTGTG AACGAGCTCT ACAGTATAAA 
GTGGGAGAGA AAATCCACGG GTTCACTGTA AACCAGGTCA CTCCTGTCCC CGAGCTGTTC 
CTGACAGCCG TGAAGCTCAG CCATGACAAC ACGGGAGCCA GATACCTGCA CCTGGCAAGG 
GAAGACAAGA ACAACTTATT CAGTGTGCAG TTCCGCACAA CCCCAATGGA TAGCACTGGG 
GTCCCACATG TTCTCGAGCA TACGGTCCTG TGCGGCTCTC AGAAGTACCC GTGCAGAGAT 
CCTTTCTTCA AAATGCTCAA CAGGTCACTG TCCACATTTA TGAATGCCAT GACAGCCAGC 
GATTACACGA TATATCCGTT TTCCACTCAA AATCCCAAAG ATTTTCAGAA CCTCCTCTCC 
GTGTATTTGG ATGCAACTTT CTTCCCCTGC TTGAGGGAAC TGGACTTCTG GCAGGAAGGA 
TGGCGTCTGG AGCATGAGAA TCCCCGAGAC CCTCAGACGC CCTTGATCTT TAAGGGGGTC 
GTCTTCAACG AGATGAAAGG GGCATTTACA GACAATGAGA GGATATTCTC CCAGCACCTG 
CAGAACAAGC TGCTTCCTGA CCACACCTAC TCCGTGGTTT CTGGAGGGGA CCCACTGTGC 
ATCCCGGAGC TCACGTGGGA ACAGCTGAAA CAGTTCCACG CTACTCATTA TCACCCAAGC 
AATGCCAGGT TCTTCACTTA TGGCAATTTT CAGCTGGAAG GACACCTGAA ACAAATTCAC 
GAAGAAGCCC TGAGTAAATT CCAGAGATTG GAGCAGAGTA CAGCAGTGCC TGCCCAGCCG 
CACTGGGATA AGCCTAGGGA ATTCCATATA ACATGTGGCC CAGATTCACT AGCTACGGAG 
ACTGCCAAGC AGACAACTGT CAGCGTTAGC TTCCTCTTAC CGGATATCAC TGACACATTT 
GAAGCCTTCA CCTTGAGCCT TCTGTCCTCC CTCCTGATTG CTGGACCCAA CTCCCCCTTC 
TACAAAGCTT TGATCGAGTC TGGACTCGGC ACAGACTTTT CTCCTGATGT TGGATATAAT 
GGCTATACAC GGGAGGCTTA CTTCAGTGTC GGGCTCCAAG GGATCGCAGA GAAAGATGTC 
AAGACGGTCA GAGAGCTCGT AGACAGGACA ATCGAAGAAG TTATAGAGAA AGGATTTGAA 
GATGATCGGA TTGAAGCTCT GCTTCATAAA ATCGAAATTC AAACGAAGCA TCAGTCAGCC 
AGCTTTGGCC TGACCCTGAC GTCATATATA GCTTCTTGCT GGAACCATGA TGGGGACCCT 
GTGGAGCTCC TGCAGATTGG AAGTCAGCTG ACTAGATTTA GGAAGTGCCT TAAGGAAAAT 
CCAAAATTTT TACAAGAAAA AGTAGAACAA TATTTTAAGA ACAATCAGCA CAAGCTGACT 
TTATCCATGA AGCCAGACGA CAAGTATTAT GAAAAGCAAA CTCAGATGGA GACAGAAAAG 
CTGGAGCAAA AGGTGAATTC TCTCTCCCCG GCGGACAAGC AGCAGATCTA CGAGAAAGGT 
TTAGAACTAC AGACGCAGCA AAGTAAACAT CAAGACGCCT CCTGCCTCCC AGCATTGAAA 
GTCTCGGACA TTGAGCCCTC CATGCCTTTC ACCAAGCTTG ACATCGGCCT TGCAGCTGGA 
GACATCCCTG TGCAGTACTG CCCACAGCCC ACCAACGGCA TGGTGTATTT CCGAGCCTTT 
TCCAGTTTAA ACACGCTGCC GGAGGACCTG AGGCCCATTG TGCCTCTCTT TTGCAGCGTG 
CTGACCAAGC TGGGTTGTGG CATCCTTAAC TACAGAGAGC AAGCCCAACA GATTGAGCTC 
AAGACAGGAG GCATGAGTGT CACGCCCCAT GTGCTCCCTG ACGACTCACA GCTGGATACC 
TACGAGCAGG GTGTGTTATT TTCATCTCTC TGCCTGGAGC GGAACCTGCC AGACATGATG 
CATCTGTGGA GCGAAATATT TAACAATCCA TGCTTTGAAG AAGAAGAACA CTTCAAAGTG 
TTGGTGAAGA TGACCGCTCA GGAGCTCTCC AATGGAATTT CAGACTCGGG GCATCTCTAT 
GCAGCCCTCA GAGCAAGCAA GACACTGACA CCTTCAGGGG ACTTGCAGGA GACCTTCAGT 
GGGATGGATC AGGTGAAGGT GATGAAAAGA ATTGCAGAGA TGACAGACAT CAAGCCCATC 
CTGAGAAAAC TGCCCCGGAT CAAGAAGTAT CTACTAAACT GTGACAACAT GAGATGCTCA 
GTGAATGCCA CCCCTCAGCA GATGCCTCAG GCAGAAAAAG AGGTGGAAAA CTTCCTTAGA 
AATGTTGGCC GAAGCAAAAA GGAACGGAAG CCTGTCCGCC CGCATATTGT CGAGAAACCC 
ACACCCAGTG GCCCCAGTGG AGCTGCACAT GTCAGTGGGT CCCAGATCGT CAGAAAATTG 
GTGACAGACC CCACCTTCAA ACCCTGCCAG ATGAAGACAC ATTTTGTGCT GCCCTTCCCT 
GTGAATTACA TTGGCGAGTG TGTCAGGACT GTCCCGTATG CTGATCCAGA CCATGCCAGC 
CTTAAGATCC TTGCCCGTCT AATGACAGCT AAATTCTTGC ATACGGAAAT TCGAGAGAAG 
GGGGGTGCTT ATGGTGGCGG TGCTAAACTC ACCCACAGTG GGATTTTCAC GCTTTACTCT 
TACAGGGATC CCAATTCCAT AGAAACACTC CAGTCTTTTG GGAAAGCTGT AGACTGGGCT 
AAGTCTGGAA AGTTCACACA GCAGGACATT GATGAAGCCA AGCTGTCTGT TTTCTCTACT 
GTGGATTCTC CTGTTGCTCC ATCCGATAAA GGAATGGACC ACTTCTTGTA TGGCCTCTCC 
GATGAGATGA AGCAGGCATA CCGAGAACAG CTCTTTGCTG TCAACCACGA CAAACTGACC 
TCTGTGAGCC ATAAATACCT TGGCATCGGG AAGAGCACAC ACGGCCTGGC TATCCTCGGA 
CCAGAGAACT CAAAAATTGC CAAAGACCCA TCATGGATCA TAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pitrm1 ( XM_006516508.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516571.1 Mus musculus pitrilysin metallepetidase 1 (Pitrm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu48299
Clone ID Related Accession (Same CDS sequence)	NM_001360106.1 , XM_006516508.1
Accession Version	XM_006516508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	presequence protease, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCGCT TCAGCGGTCG GCGGGGACTC TGCGCTGTAC AGCGGCTGAG CTGCGGGGTA 
CACCACAGAG TATGGAGGGA GAAGAGTGAC CAAGCCTGTG AACGAGCTCT ACAGTATAAA 
GTGGGAGAGA AAATCCACGG GTTCACTGTA AACCAGGTCA CTCCTGTCCC CGAGCTGTTC 
CTGACAGCCG TGAAGCTCAG CCATGACAAC ACGGGAGCCA GATACCTGCA CCTGGCAAGG 
GAAGACAAGA ACAACTTATT CAGTGTGCAG TTCCGCACAA CCCCAATGGA TAGCACTGGG 
GTCCCACATG TTCTCGAGCA TACGGTCCTG TGCGGCTCTC AGAAGTACCC GTGCAGAGAT 
CCTTTCTTCA AAATGCTCAA CAGGTCACTG TCCACATTTA TGAATGCCAT GACAGCCAGC 
GATTACACGA TATATCCGTT TTCCACTCAA AATCCCAAAG ATTTTCAGAA CCTCCTCTCC 
GTGTATTTGG ATGCAACTTT CTTCCCCTGC TTGAGGGAAC TGGACTTCTG GCAGGAAGGA 
TGGCGTCTGG AGCATGAGAA TCCCCGAGAC CCTCAGACGC CCTTGATCTT TAAGGGGGTC 
GTCTTCAACG AGATGAAAGG GGCATTTACA GACAATGAGA GGATATTCTC CCAGCACCTG 
CAGAACAAGC TGCTTCCTGA CCACACCTAC TCCGTGGTTT CTGGAGGGGA CCCACTGTGC 
ATCCCGGAGC TCACGTGGGA ACAGCTGAAA CAGTTCCACG CTACTCATTA TCACCCAAGC 
AATGCCAGGT TCTTCACTTA TGGCAATTTT CAGCTGGAAG GACACCTGAA ACAAATTCAC 
GAAGAAGCCC TGAGTAAATT CCAGAGATTG GAGCAGAGTA CAGCAGTGCC TGCCCAGCCG 
CACTGGGATA AGCCTAGGGA ATTCCATATA ACATGTGGCC CAGATTCACT AGCTACGGAG 
ACTGCCAAGC AGACAACTGT CAGCGTTAGC TTCCTCTTAC CGGATATCAC TGACACATTT 
GAAGCCTTCA CCTTGAGCCT TCTGTCCTCC CTCCTGATTG CTGGACCCAA CTCCCCCTTC 
TACAAAGCTT TGATCGAGTC TGGACTCGGC ACAGACTTTT CTCCTGATGT TGGATATAAT 
GGCTATACAC GGGAGGCTTA CTTCAGTGTC GGGCTCCAAG GGATCGCAGA GAAAGATGTC 
AAGACGGTCA GAGAGCTCGT AGACAGGACA ATCGAAGAAG TTATAGAGAA AGGATTTGAA 
GATGATCGGA TTGAAGCTCT GCTTCATAAA ATCGAAATTC AAACGAAGCA TCAGTCAGCC 
AGCTTTGGCC TGACCCTGAC GTCATATATA GCTTCTTGCT GGAACCATGA TGGGGACCCT 
GTGGAGCTCC TGCAGATTGG AAGTCAGCTG ACTAGATTTA GGAAGTGCCT TAAGGAAAAT 
CCAAAATTTT TACAAGAAAA AGTAGAACAA TATTTTAAGA ACAATCAGCA CAAGCTGACT 
TTATCCATGA AGCCAGACGA CAAGTATTAT GAAAAGCAAA CTCAGATGGA GACAGAAAAG 
CTGGAGCAAA AGGTGAATTC TCTCTCCCCG GCGGACAAGC AGCAGATCTA CGAGAAAGGT 
TTAGAACTAC AGACGCAGCA AAGTAAACAT CAAGACGCCT CCTGCCTCCC AGCATTGAAA 
GTCTCGGACA TTGAGCCCTC CATGCCTTTC ACCAAGCTTG ACATCGGCCT TGCAGCTGGA 
GACATCCCTG TGCAGTACTG CCCACAGCCC ACCAACGGCA TGGTGTATTT CCGAGCCTTT 
TCCAGTTTAA ACACGCTGCC GGAGGACCTG AGGCCCATTG TGCCTCTCTT TTGCAGCGTG 
CTGACCAAGC TGGGTTGTGG CATCCTTAAC TACAGAGAGC AAGCCCAACA GATTGAGCTC 
AAGACAGGAG GCATGAGTGT CACGCCCCAT GTGCTCCCTG ACGACTCACA GCTGGATACC 
TACGAGCAGG GTGTGTTATT TTCATCTCTC TGCCTGGAGC GGAACCTGCC AGACATGATG 
CATCTGTGGA GCGAAATATT TAACAATCCA TGCTTTGAAG AAGAAGAACA CTTCAAAGTG 
TTGGTGAAGA TGACCGCTCA GGAGCTCTCC AATGGAATTT CAGACTCGGG GCATCTCTAT 
GCAGCCCTCA GAGCAAGCAA GACACTGACA CCTTCAGGGG ACTTGCAGGA GACCTTCAGT 
GGGATGGATC AGGTGAAGGT GATGAAAAGA ATTGCAGAGA TGACAGACAT CAAGCCCATC 
CTGAGAAAAC TGCCCCGGAT CAAGAAGTAT CTACTAAACT GTGACAACAT GAGATGCTCA 
GTGAATGCCA CCCCTCAGCA GATGCCTCAG GCAGAAAAAG AGGTGGAAAA CTTCCTTAGA 
AATGTTGGCC GAAGCAAAAA GGAACGGAAG CCTGTCCGCC CGCATATTGT CGAGAAACCC 
ACACCCAGTG GCCCCAGTGG AGCTGCACAT GTCAGTGGGT CCCAGATCGT CAGAAAATTG 
GTGACAGACC CCACCTTCAA ACCCTGCCAG ATGAAGACAC ATTTTGTGCT GCCCTTCCCT 
GTGAATTACA TTGGCGAGTG TGTCAGGACT GTCCCGTATG CTGATCCAGA CCATGCCAGC 
CTTAAGATCC TTGCCCGTCT AATGACAGCT AAATTCTTGC ATACGGAAAT TCGAGAGAAG 
GGGGGTGCTT ATGGTGGCGG TGCTAAACTC ACCCACAGTG GGATTTTCAC GCTTTACTCT 
TACAGGGATC CCAATTCCAT AGAAACACTC CAGTCTTTTG GGAAAGCTGT AGACTGGGCT 
AAGTCTGGAA AGTTCACACA GCAGGACATT GATGAAGCCA AGCTGTCTGT TTTCTCTACT 
GTGGATTCTC CTGTTGCTCC ATCCGATAAA GGAATGGACC ACTTCTTGTA TGGCCTCTCC 
GATGAGATGA AGCAGGCATA CCGAGAACAG CTCTTTGCTG TCAACCACGA CAAACTGACC 
TCTGTGAGCC ATAAATACCT TGGCATCGGG AAGAGCACAC ACGGCCTGGC TATCCTCGGA 
CCAGAGAACT CAAAAATTGC CAAAGACCCA TCATGGATCA TAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516571.1
CDS	49..3156
Translation	MWRFSGRRGLCAVQRLSCGVHHRVWREKSDQACERALQYKVGEKIHGFTVNQVTPVPELFLTAVKLSHDNTGARYLHLAREDKNNLFSVQFRTTPMDSTGVPHVLEHTVLCGSQKYPCRDPFFKMLNRSLSTFMNAMTASDYTIYPFSTQNPKDFQNLLSVYLDATFFPCLRELDFWQEGWRLEHENPRDPQTPLIFKGVVFNEMKGAFTDNERIFSQHLQNKLLPDHTYSVVSGGDPLCIPELTWEQLKQFHATHYHPSNARFFTYGNFQLEGHLKQIHEEALSKFQRLEQSTAVPAQPHWDKPREFHITCGPDSLATETAKQTTVSVSFLLPDITDTFEAFTLSLLSSLLIAGPNSPFYKALIESGLGTDFSPDVGYNGYTREAYFSVGLQGIAEKDVKTVRELVDRTIEEVIEKGFEDDRIEALLHKIEIQTKHQSASFGLTLTSYIASCWNHDGDPVELLQIGSQLTRFRKCLKENPKFLQEKVEQYFKNNQHKLTLSMKPDDKYYEKQTQMETEKLEQKVNSLSPADKQQIYEKGLELQTQQSKHQDASCLPALKVSDIEPSMPFTKLDIGLAAGDIPVQYCPQPTNGMVYFRAFSSLNTLPEDLRPIVPLFCSVLTKLGCGILNYREQAQQIELKTGGMSVTPHVLPDDSQLDTYEQGVLFSSLCLERNLPDMMHLWSEIFNNPCFEEEEHFKVLVKMTAQELSNGISDSGHLYAALRASKTLTPSGDLQETFSGMDQVKVMKRIAEMTDIKPILRKLPRIKKYLLNCDNMRCSVNATPQQMPQAEKEVENFLRNVGRSKKERKPVRPHIVEKPTPSGPSGAAHVSGSQIVRKLVTDPTFKPCQMKTHFVLPFPVNYIGECVRTVPYADPDHASLKILARLMTAKFLHTEIREKGGAYGGGAKLTHSGIFTLYSYRDPNSIETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSTVDSPVAPSDKGMDHFLYGLSDEMKQAYREQLFAVNHDKLTSVSHKYLGIGKSTHGLAILGPENSKIAKDPSWIIK

Target ORF information:

RefSeq Version	XM_006516508.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pitrilysin metallepetidase 1 (Pitrm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516508.1

ORF Insert Sequence:

ATGTGGCGCT TCAGCGGTCG GCGGGGACTC TGCGCTGTAC AGCGGCTGAG CTGCGGGGTA 
CACCACAGAG TATGGAGGGA GAAGAGTGAC CAAGCCTGTG AACGAGCTCT ACAGTATAAA 
GTGGGAGAGA AAATCCACGG GTTCACTGTA AACCAGGTCA CTCCTGTCCC CGAGCTGTTC 
CTGACAGCCG TGAAGCTCAG CCATGACAAC ACGGGAGCCA GATACCTGCA CCTGGCAAGG 
GAAGACAAGA ACAACTTATT CAGTGTGCAG TTCCGCACAA CCCCAATGGA TAGCACTGGG 
GTCCCACATG TTCTCGAGCA TACGGTCCTG TGCGGCTCTC AGAAGTACCC GTGCAGAGAT 
CCTTTCTTCA AAATGCTCAA CAGGTCACTG TCCACATTTA TGAATGCCAT GACAGCCAGC 
GATTACACGA TATATCCGTT TTCCACTCAA AATCCCAAAG ATTTTCAGAA CCTCCTCTCC 
GTGTATTTGG ATGCAACTTT CTTCCCCTGC TTGAGGGAAC TGGACTTCTG GCAGGAAGGA 
TGGCGTCTGG AGCATGAGAA TCCCCGAGAC CCTCAGACGC CCTTGATCTT TAAGGGGGTC 
GTCTTCAACG AGATGAAAGG GGCATTTACA GACAATGAGA GGATATTCTC CCAGCACCTG 
CAGAACAAGC TGCTTCCTGA CCACACCTAC TCCGTGGTTT CTGGAGGGGA CCCACTGTGC 
ATCCCGGAGC TCACGTGGGA ACAGCTGAAA CAGTTCCACG CTACTCATTA TCACCCAAGC 
AATGCCAGGT TCTTCACTTA TGGCAATTTT CAGCTGGAAG GACACCTGAA ACAAATTCAC 
GAAGAAGCCC TGAGTAAATT CCAGAGATTG GAGCAGAGTA CAGCAGTGCC TGCCCAGCCG 
CACTGGGATA AGCCTAGGGA ATTCCATATA ACATGTGGCC CAGATTCACT AGCTACGGAG 
ACTGCCAAGC AGACAACTGT CAGCGTTAGC TTCCTCTTAC CGGATATCAC TGACACATTT 
GAAGCCTTCA CCTTGAGCCT TCTGTCCTCC CTCCTGATTG CTGGACCCAA CTCCCCCTTC 
TACAAAGCTT TGATCGAGTC TGGACTCGGC ACAGACTTTT CTCCTGATGT TGGATATAAT 
GGCTATACAC GGGAGGCTTA CTTCAGTGTC GGGCTCCAAG GGATCGCAGA GAAAGATGTC 
AAGACGGTCA GAGAGCTCGT AGACAGGACA ATCGAAGAAG TTATAGAGAA AGGATTTGAA 
GATGATCGGA TTGAAGCTCT GCTTCATAAA ATCGAAATTC AAACGAAGCA TCAGTCAGCC 
AGCTTTGGCC TGACCCTGAC GTCATATATA GCTTCTTGCT GGAACCATGA TGGGGACCCT 
GTGGAGCTCC TGCAGATTGG AAGTCAGCTG ACTAGATTTA GGAAGTGCCT TAAGGAAAAT 
CCAAAATTTT TACAAGAAAA AGTAGAACAA TATTTTAAGA ACAATCAGCA CAAGCTGACT 
TTATCCATGA AGCCAGACGA CAAGTATTAT GAAAAGCAAA CTCAGATGGA GACAGAAAAG 
CTGGAGCAAA AGGTGAATTC TCTCTCCCCG GCGGACAAGC AGCAGATCTA CGAGAAAGGT 
TTAGAACTAC AGACGCAGCA AAGTAAACAT CAAGACGCCT CCTGCCTCCC AGCATTGAAA 
GTCTCGGACA TTGAGCCCTC CATGCCTTTC ACCAAGCTTG ACATCGGCCT TGCAGCTGGA 
GACATCCCTG TGCAGTACTG CCCACAGCCC ACCAACGGCA TGGTGTATTT CCGAGCCTTT 
TCCAGTTTAA ACACGCTGCC GGAGGACCTG AGGCCCATTG TGCCTCTCTT TTGCAGCGTG 
CTGACCAAGC TGGGTTGTGG CATCCTTAAC TACAGAGAGC AAGCCCAACA GATTGAGCTC 
AAGACAGGAG GCATGAGTGT CACGCCCCAT GTGCTCCCTG ACGACTCACA GCTGGATACC 
TACGAGCAGG GTGTGTTATT TTCATCTCTC TGCCTGGAGC GGAACCTGCC AGACATGATG 
CATCTGTGGA GCGAAATATT TAACAATCCA TGCTTTGAAG AAGAAGAACA CTTCAAAGTG 
TTGGTGAAGA TGACCGCTCA GGAGCTCTCC AATGGAATTT CAGACTCGGG GCATCTCTAT 
GCAGCCCTCA GAGCAAGCAA GACACTGACA CCTTCAGGGG ACTTGCAGGA GACCTTCAGT 
GGGATGGATC AGGTGAAGGT GATGAAAAGA ATTGCAGAGA TGACAGACAT CAAGCCCATC 
CTGAGAAAAC TGCCCCGGAT CAAGAAGTAT CTACTAAACT GTGACAACAT GAGATGCTCA 
GTGAATGCCA CCCCTCAGCA GATGCCTCAG GCAGAAAAAG AGGTGGAAAA CTTCCTTAGA 
AATGTTGGCC GAAGCAAAAA GGAACGGAAG CCTGTCCGCC CGCATATTGT CGAGAAACCC 
ACACCCAGTG GCCCCAGTGG AGCTGCACAT GTCAGTGGGT CCCAGATCGT CAGAAAATTG 
GTGACAGACC CCACCTTCAA ACCCTGCCAG ATGAAGACAC ATTTTGTGCT GCCCTTCCCT 
GTGAATTACA TTGGCGAGTG TGTCAGGACT GTCCCGTATG CTGATCCAGA CCATGCCAGC 
CTTAAGATCC TTGCCCGTCT AATGACAGCT AAATTCTTGC ATACGGAAAT TCGAGAGAAG 
GGGGGTGCTT ATGGTGGCGG TGCTAAACTC ACCCACAGTG GGATTTTCAC GCTTTACTCT 
TACAGGGATC CCAATTCCAT AGAAACACTC CAGTCTTTTG GGAAAGCTGT AGACTGGGCT 
AAGTCTGGAA AGTTCACACA GCAGGACATT GATGAAGCCA AGCTGTCTGT TTTCTCTACT 
GTGGATTCTC CTGTTGCTCC ATCCGATAAA GGAATGGACC ACTTCTTGTA TGGCCTCTCC 
GATGAGATGA AGCAGGCATA CCGAGAACAG CTCTTTGCTG TCAACCACGA CAAACTGACC 
TCTGTGAGCC ATAAATACCT TGGCATCGGG AAGAGCACAC ACGGCCTGGC TATCCTCGGA 
CCAGAGAACT CAAAAATTGC CAAAGACCCA TCATGGATCA TAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Placentation defects are highly prevalent in embryonic lethal mouse mutants.
Nature555(7697)463-468(2018 03)
Perez-Garcia V,Fineberg E,Wilson R,Murray A,Mazzeo CI,Tudor C,Sienerth A,White JK,Tuck E,Ryder EJ,Gleeson D,Siragher E,Wardle-Jones H,Staudt N,Wali N,Collins J,Geyer S,Busch-Nentwich EM,Galli A,Smith JC,Robertson E,Adams DJ,Weninger WJ,Mohun T,Hemberger M

Defective PITRM1 mitochondrial peptidase is associated with Aβ amyloidotic neurodegeneration.
EMBO molecular medicine8(3)176-90(2016 Mar)
Brunetti D,Torsvik J,Dallabona C,Teixeira P,Sztromwasser P,Fernandez-Vizarra E,Cerutti R,Reyes A,Preziuso C,D'Amati G,Baruffini E,Goffrini P,Viscomi C,Ferrero I,Boman H,Telstad W,Johansson S,Glaser E,Knappskog PM,Zeviani M,Bindoff LA

The Mitochondrial Peptidase Pitrilysin Degrades Islet Amyloid Polypeptide in Beta-Cells.
PloS one10(7)e0133263(2015)
Guan H,Chow KM,Song E,Verma N,Despa F,Hersh LB

The metalloendopeptidase gene Pitrm1 is regulated by hedgehog signaling in the developing mouse limb and is expressed in muscle progenitors.
Developmental dynamics : an official publication of the American Association of Anatomists238(12)3175-84(2009 Dec)
Town L,McGlinn E,Fiorenza S,Metzis V,Butterfield NC,Richman JM,Wicking C

Fine-mapping gene-by-diet interactions on chromosome 13 in a LG/J x SM/J murine model of obesity.
Diabetes54(6)1863-72(2005 Jun)
Ehrich TH,Hrbek T,Kenney-Hunt JP,Pletscher LS,Wang B,Semenkovich CF,Cheverud JM

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Pitrm1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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