Ubr4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ubr4
Entrez Gene ID	69116
Full Name	ubiquitin protein ligase E3 component n-recognin 4
Synonyms	1810009A16Rik,A930005E13Rik,D930005K06Rik,Gm1032,Gm1666,N28143,RBAF600,Zubr1,mKIAA0462,p600
General protein information	Preferred Names ubiquitin protein ligase E3 component n-recognin 4 Names E3 ubiquitin-protein ligase UBR4 N-recognin-4 RING-type E3 ubiquitin transferase UBR4 retinoblastoma-associated factor 600 zinc finger UBR1-type protein 1 zinc finger, UBR1 type 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001160319.1	NP_001153791.1	E3 ubiquitin-protein ligase UBR4

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBR4	NP_065816.2
Rattus norvegicus (Norway rat)	Ubr4	NP_001034115.1
Caenorhabditis elegans (roundworm)	C44E4.1	NP_740838.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001157	XP_322005.5
Canis lupus familiaris (dog)	UBR4	XP_005617954.1
Pan troglodytes (chimpanzee)	UBR4	XP_513158.2
Macaca mulatta (Rhesus monkey)	LOC702449	XP_002802257.1
Mus musculus (house mouse)	Ubr4	NP_001153791.1
Drosophila melanogaster (fruit fly)	poe	NP_001260222.1
Bos taurus (cattle)	UBR4	NP_001192721.1
Gallus gallus (chicken)	UBR4	XP_417626.4
Danio rerio (zebrafish)	ubr4	XP_005162254.1
Xenopus tropicalis (tropical clawed frog)	ubr4	XP_002943596.2

Gene Ontology
Bibliography

Related articles in PubMed

The N-recognin UBR4 of the N-end rule pathway is targeted to and required for the biogenesis of the early endosome.
Kim ST, Lee YJ, Tasaki T, Mun SR, Hwang J, Kang MJ, Ganipisetti S, Yi EC, Kim BY, Kwon YT
Journal of cell science131(17)(2018 09)

The N-recognin UBR4 of the N-end rule pathway is required for neurogenesis and homeostasis of cell surface proteins.
Kim ST, Lee YJ, Tasaki T, Hwang J, Kang MJ, Yi EC, Kim BY, Kwon YT
PloS one13(8)e0202260(2018)

The ubiquitin ligase Ubr4 controls stability of podocin/MEC-2 supercomplexes.
Rinschen MM, Bharill P, Wu X, Kohli P, Reinert MJ, Kretz O, Saez I, Schermer B, Höhne M, Bartram MP, Aravamudhan S, Brooks BR, Vilchez D, Huber TB, Müller RU, Krüger M, Benzing T
Human molecular genetics25(7)1328-44(2016 Apr)

p600 regulates spindle orientation in apical neural progenitors and contributes to neurogenesis in the developing neocortex.
Belzil C, Asada N, Ishiguro K, Nakaya T, Parsons K, Pendolino V, Neumayer G, Mapelli M, Nakatani Y, Sanada K, Nguyen MD
Biology open3(6)475-85(2014 May)

Time-of-day- and light-dependent expression of ubiquitin protein ligase E3 component N-recognin 4 (UBR4) in the suprachiasmatic nucleus circadian clock.
Ling HH, Beaulé C, Chiang CK, Tian R, Figeys D, Cheng HY
PloS one9(8)e103103(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ubr4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ubr4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08040	NM_001160319.1 Latest version!	Mus musculus ubiquitin protein ligase E3 component n-recognin 4 (Ubr4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ubr4 ( NM_001160319.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153791.1 Mus musculus ubiquitin protein ligase E3 component n-recognin 4 (Ubr4), mRNA.

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CloneID	OMu08040
Clone ID Related Accession (Same CDS sequence)	NM_001160319.1
Accession Version	NM_001160319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 15543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase UBR4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL807833.5, AK080556.1, BC057625.1 and BC051096.1. On or before May 21, 2009 this sequence version replaced XM_001479738.1, XM_001479746.1, XM_001479450.1, XM_001479455.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGA GCGGCGGCGA AGAGGCGGCG GCAGCAGCTC CAGCGCCCGG AGCCCCGGCG 
ACCGGGCAGG ACACGACCCC GGGCTGGGAG GTGGCGGTGC GGCCCCTCCT GTCCGCGTCC 
TACTCCGCCT TCGAGATGAA GGAGTTGCCG CAGCTGGTGG CCTCAGTCAT CGAGAGTGAG 
TCAGAAATCC TGCACCACGA GAAGCAGTAC GAGCCGTTCT ACTCCTCCTT TGTCGCACTG 
TCCACGCACT ACATCACCAC GGTCTGCAGC CTCATTCCCC GGAACCAGCT TCAGTCGGTG 
GCAGCTGCCT GTAAAGTTCT CATTGAGTTT TCCCTCCTGC GTCTGGAGAA CCCTGACGAG 
GCATGTGCTG TATCCCAGAA ACACTTGATT CTGCTGATAA AAGGCCTGTG CACTGGCTGT 
AGCCGACTAG ACAGAACAGA GATTATCACC TTTACAGCAA TGATGAAGTC CGCCAAGCTT 
CCACAGACAG TGAAGACGCT TTCAGACGTG GAAGATCAGA AAGAGCTGGC CTCCCCAGTA 
AGCCCCGAGC TCAGGCAGAA GGAGGTACAG ATGAACTTTT TGAACCAGCT GACCTCAGTT 
TTTAACCCTA GAACTGTACC ATCTCCTCCC ATCAGTCCAC AGGCTCTGGT GGAAGGAGAA 
AATGATGAGC AGTCATCTCC AGATCAGGTC TCTGCTGCCA AGACCAAGAG TGTGTTCATA 
GCTCAGAATG TGGCTAGCCT TCAAGAGCTT GGGGGTTCCG AGAAGCTACT CCGAGTATGC 
CTGAACCTGC CCTATTTCCT GCGCTACATC AATCGGTTTC AGGATGCTGT GGTGGCCAAT 
TCCTTCTTCA TCATGCCAGC AACAGTAGCT GATGCCACCG CTGTTCGAAA TGGTTTTCAC 
TCACTGGTGA TTGATGTAAC CATGGCCTTG GATACCCTTT CCCTGCCTGT GCTGGAACCC 
CTCAATCCTT CTCGTCTGCA AGATGTGACA GTGCTCAGCC TCAGCTGTCT GTATGCAGGT 
GTGAGTGTAG CCACGTGTAT GGCCATCCTT CATGTGGGTA GTGCCCAGCA AGTGCGGACA 
GGGTCCACAA GCTCCAAAGA AGATGACTAC GAAAGTGATG CAGCCACGAT TGTTCAGAAA 
TGTCTTGAGA TCTACGACAT GATTGGACAG GCCATCAGCA GCTCCCGCCG CGCAGGTGGT 
GAGCACTTCC AGAACTTCCA GCTGTTGGGC GCCTGGTGCC TGCTTAATAG CCTCTTCCTC 
ATACTGAACC TCAGCCCCAC AGCCCTGGCT GACAAGGGGA AAGAGAAGGA CCCATTGGCT 
GCCCTTCGCG TCAGAGACAT CCTTTCTCGT ACAAAGGAGG GAGTGGGCTC TCCCAAACTG 
GGGCCTGGCA AAGGGCATCA GGGATTTGGG GTACTCTCGG TGATACTGGC AAACCATGCC 
ATCAAGCTGC TGGCGTCTCT CTTTCAAGAC TTGCAGGTGG AGGCACTCCA CAAGGGCTGG 
GAGACAGATG GCCCCCCTGC AGTCCTGAGT ATTATGGCCC AGAGCACCTC CACACAAAGG 
ATTCAACGGC TGATTGACTC TGTCCCACTG ACAAACCTGC TCTTGACTTT ACTTTCAACT 
TCATACAGAA AGGCGTGTGT CCTGCAGCGG CAGAGGAAGG GCTCCATGAG CAGTGATGCC 
AGCGCCTCCA CCGACTCCAA CACGTACTAT GAGGATGACT TCAGTAGCAC AGAGGAGGAC 
AGCAGCCAAG ATGATGACAG TGAGCCTATT TTGGGGCAGT GGTTTGAGGA GACCATCTCT 
CCCAGTAAAG AAAAAGCTGC GCCTCCACCT CCTCCACCAC CCCCTCCCCT GGAGAGCTCT 
CCTCGGGTTA AAAGCCCCAA TAAGCAGGCA TCTGGTGAGA AGGGCAACAT CTTGGCCAGC 
CGCAAAGACC CTGAGTTGTT CTCAGGCCTG GCTTCCAACA TCCTGAACTT CATCACGACT 
TCCATGCTGA ACTCTCGAAA CAGTTTCATC CGGAGCTATC TGAGCGCCTC TCTCTCAGAG 
CACCACATGG CCACTCTGGC CAGCATCATT AAGGAGGTGG ACAAAGATGG ACTCAAGGGG 
TCATCAGATG AGGACTTTGC TGCTGCTCTC TATCACTTCA ACCACTCGCT GGTGACTTCT 
GACCTACAGT CCCCAAACCT GCAGAATACA CTGCTCCAGC AGCTGGGAGT TGCTCCTTTC 
TCCGAAGGCC CTTGGCCCCT GTACATTCAC CCCCAAGGCC TCTCTGTGCT TTCTCGCCTC 
CTGCTTATCT GGCAGCATAA AGCTGGAGCC CAAGGTGACC CTGATGTCCC TGAATGCCTG 
AAAGTGTGGG ACAGGTTTTT AACTACCATG AAGCAGAATG CACTGCAGGG GGTGGTGCCC 
AGTGAGACAG AAGACCTGAA CGTTGAGCAC CTGCAGCTGC TGCTGCTCAT CTTCCACAGC 
TTCTCTGAGA AGGGCCGCAG AGCCATCTTG ACCATGCTTG TGCAGAGCAT CCAGGAGCTG 
AGTGTTAACA TGGAGGTGCA GATGCGCACT GCACCGCTGA TCCTGGCCCG CCTCCTCTTG 
ATCTTTGACT ACCTACTTCA TCAGTACTCC AAAGCCCCCG TGTATCTGTT TGAGCAGGTG 
CAGCACAATC TGCTAAGTCC CCCTTTTGGG TGGGCAAGTG GATCCCAAGA CAGCAGCAGC 
CGCCGAGCCA ACACTCCTCT GTATCATGGC TTCAAGGAAG TAGAAGAAAA TTGGTCCAAA 
CATTTTTCAT CAGATGCGGC CCCACAACCC AGGTTCTACT GCGTCCTGTC CACAGAGGCC 
TCTGAGGAGG ATTTGAACCG GCTGGATTCT GAGGCATGTG AAGTTCTTTT CTCCAAGCCT 
GTCAAGTACG ATGAACTTTA TTCCTCACTG ACTACCCTGC TCGCGGCTGG ATCTCAGCTG 
GATACAACCA GGAGGAAGGA AAAGAAGAAC GTGACAGCCC TGGAGGCCTG CGCCCTGCAG 
TATTACTTCT TGATTCTCTG GAGGATCTTA GGAATTTTGC CACCTTCAAA GACTTACATG 
AACCAGCTGG CCATGAATTC ACCTGAGATG AGTGAATGTG ACATCTTACA CACTCTGCGA 
TGGTCGTCTC GCCTCCGGAT CAGTTCCTAT GTCAGCTGGA TAAAGGATCA CCTTATCAAA 
CAAGGCATGA AGCCCGAGCA TGCGGGCTCT CTCATAGAAC TGGCTGCCAG CAAGTGTAGT 
TCAGTGAAGT ACGATGTTGA GATTGTAGAG GAGTACTTTG CCCGACAGAT CTCGTCCTTC 
TGCTCCATTG ACTGCACTGC CGTCCTCCAG CTGCATGAGA TCCCCAGTCT GCAGTCCATC 
TACACCCTGG ATGCCGCTGT CTCGAAGGTC CAGGTCTCTC TGGATGAGCA CTTCTCTAAG 
ATGGCCGCAG AGACCGACCC CCATAAGTCA TCTGAGATCA CCAAGAACCT CCTTCCAGCT 
ACGCTGCAGC TCATTGACAC CTATGCCTCG TTCACCAGAG CCTATCTGCT GCAGAACCTT 
AATGAAGAGG GGTCCACTGA GAAGCCCTCC CAGGAGAAGC TGCATGGCTT TGCAGCTGTC 
TTGGCTATCG GCTCCAGTAG GTGCAAGGCA AACACACTGG GACCAACTCT AGTGCAGAAT 
TTACCGTCAT CAGTGCAGTC TGTCTGTGAG TCCTGGAATA ACATCAATAC CAATGAGTTT 
CCCAACATTG GATCCTGGCG CAATGCATTT GCCAACGACA CCATCCCGTC CGAGAGTTAC 
ATCAGTGCCG TGCAGGCTGC TCACCTGGGG ACTCTGTGTG GCCAGAGTCT GCCCCTGGCG 
GCTTCCCTGA AACATACCCT CCTCTCCCTG GTCAGGTTGA CTGGGGATCT GATTGTGTGG 
TCAGATGAGA TGAACCCAGC ACAGGTGATC CGGACTCTGT TACCGCTTCT CTTGGAGTCA 
AGCACAGAGA GCGCTGCTGA GATCAGCAGT AACTCTCTGG AGCGCATCTT GGGACCTGCG 
GAGTCTGATG AGTTCCTGGC TCGTGTGTAT GAGAAGCTGA TCACGGGATG TTACAACATT 
CTGGCCAACC ATGCAGATCC CAACAGTGGG CTGGATGAGT CCATCCTGGA GGAGTGTTTA 
CAGTATCTGG AGAAGCAGCT GGAGAGCAGC CAGGCCCGGA AGGCCATGGA GGAGTTCTTC 
TCTGATGGTG GGGAGCTGGT GCAGATCATG ATGGCCACAG CCAATGAGGA CCTCTCTGCT 
AAATTCTGTA ACCGAGTCTT GAAATTCTTC ACCAAACTCT TCCAGCTGAC TGAGAAGAGC 
CCTAACCCGA GCCTCCTTCA CCTCTGTGGC TCTCTCGCCC AGCTTGCGTG TGTGGAGCCG 
GTACGCCTGC AGGCCTGGCT CACCCGCATG ACAACATCGC CCCCGAAGGA TTCTGACCAG 
CTGGAGGTCA TTCAGGAGAA CCGGCAGCTG CTGCAGTTAC TGACCACGTA CATCGTTCGG 
GAGAACAGCC AAGTTGGAGA AGGTGTGTGT GCTGTGCTTC TGGGCACCCT GACCCCCATG 
GCAACAGATA TGCTGGCGAA CGGTGATGGG ACCGGCTTCC CTGAGCTCAT GGTTGTGATG 
GCCACTCTAG CCAGTGCCGG GCAAGGAGCT GGCCACCTTC AGCTTCACAA TGCTGCAGTG 
GACTGGCTGG GCAGATGCAA GAAATACCTG TCACAGAAGA ACGTGGTTGA GAAGCTGAAT 
GCCAATGTAA TGCATGGAAA GCATGTGATG GTCTTGGAGT GCACATGTCA TATTATGTCT 
TACCTGGCCG ATGTCACAAA TGCCCTGAGC CAGAGTAATG GTCAAGGCCC AAGCCATCTG 
TCAGTGGATG GGGAAGAGCG TGCCATTGAG GTGGATTCAG ACTGGGTGGA AGAGTTAGCG 
GTGGAGGAGG AAGACTCCCA GGCTGAGGAC TCTGATGAAG ATTCTCTTTG CAATAAGCTG 
TGTACCTTCA CCATCACACA GAAGGAGTTT ATGAACCAGC ATTGGTACCA CTGTCATACC 
TGTAAGATGG TGGATGGGGT GGGTGTGTGC ACAGTGTGTG CAAAGGTATG CCATAAGGAC 
CATGAGATTT CCTACGCCAA GTATGGTTCC TTTTTCTGTG ACTGTGGAGC CAAGGAAGAT 
GGCAGCTGCT TGGCCCTGGT GAAGCGAACT CCTAGCAGTG GCATGAGCTC TACCATGAAG 
GAGTCAGCAT TTCAGAGCGA ACCTAGGGTT TCCGAAAGTC TGGTGCGCCA TGCCAGCACC 
TCTCCTGCTG ACAAGGCCAA GGTCACCATC AGTGATGGGA AGGTTACAGA TGAAGAGAAG 
CCCAAGAAGA GCAGCCTGTG CCGTACAGTT GAGGGGTGTC GTGAGGAGCT GCAGAATCAG 
GCCAATTTCT CCTTCGCTCC TCTCGTGTTA GACATGCTCA GTTTCCTCAT GGATGCCATT 
CAGACCAACT TTCAGCAGGC TTCAGCCGTG GGGAGCAGCA GCAGGGCCCA GCAGGCCCTC 
AGTGAACTGC ACACAGTGGA CAAGGGTGTG GAGATGACAG ACCAGCTGAT GGTTCCCACC 
TTAGGCTCAC AGGAAGGTGC CTTTGAGAAT GTTCGGATGA ATTACAGCGG AGACCAGGGC 
CAGACTATAC GGCAGCTGAT CAGTGCCCAC GTGCTGAGAC GGGTGGCTAT GTGTGTGCTT 
TCTTCCCCCC ATGGGCGCCG CCAGCATTTG GCTGTTAGCC ATGAGAAGGG CAAGATCACA 
GTGCTGCAGC TCTCAGCGCT CCTGAAGCAA GCAGATTCCA GTAAGAGGAA GCTGACGCTA 
ACCCGCTTGG CTTCTGCCCC AGTTCCCTTC ACAGTGCTGA GTCTTACTGG AAACCCCTGC 
AAGGAGGACT ACCTGGCAGT GTGTGGGCTG AAGGACTGCC ACGTGCTCAC CTTCAGCAGC 
TCAGGCTCTG TCTCCGATCA CTTAGTGTTA CATCCCCAGT TGGCCACAGG GAACTTCATC 
ATCAAAGCTG TGTGGCTCCC TGGTTCACAG ACTGAGCTAG CCATCGTCAC CGCAGACTTC 
GTCAAGATTT ACGACCTGTC TATTGATGCC TTGAGTCCAA CCTTCTACTT CCTCCTGCCG 
AGCTCAAAGA TAAGAGACGT TACCTTCCTT TTCAACGAGG AAGGCAAGAA CATCATTGTC 
ATCATGTCCT CTGCTGGGTA CATGTACACA CAACTCATGG AGGAGGCCAG CAGTGCCCAG 
CAGGGCCCCT TCTATGTCAC TAATGTGCTG GAAATAAATC ACGAGGACCT GAAGGACAGT 
AACAGCCAGG TGGCAGGCGG TGGCGTGTCT GTGTACTACT CACACGTGTT GCAGATGCTT 
TTCTTCAGCT ACAGTCAGGG CAGGTCCTTT GCAGCCACTG TCAGCAGGAG CACCCTGGAG 
GTGTTGCAGC TCTTCCCCAT CAACATCAAA AGCTCCAATG GTGGCAGTAA GACTTCTCCT 
GCCCTTTGCC AGTGGTCCGA GGTGATGAAC CACCCTGGCT TGGTGTGTTG TGTTCAGCAG 
ACTACTGGCG TGCCTCTGGT GGTCATGGTG AAGCCAGGCA CTTTCCTCAT CCAGGAGATT 
AAGACTCTTC CCGCCAAAGC AAAGATCCAA GACATGGTTG CCATTAGGCA CACTGCCTGC 
AATGAGCAGC AACGTACAAC CATGATCCTG CTGTGCGAGG ACGGCAGCCT GCGGATTTAC 
ATGGCCAATG TAGAGAACAC CTCTTACTGG CTCCAGCCGT CTCTGCAGCC CAGCAGTGTC 
ATCAGCATCA TGAAACCTGT GCGGAAGCGC AAAACAGCTA CCATCACAGC CCGCACATCC 
AGCCAGGTCA CCTTCCCCAT TGACTTTTTT GAACACAACC AGCAGCTAAC AGATGTGGAG 
TTTGGCGGTA ATGACCTCTT GCAAGTCTAC AATGCACAGC AGATAAAGCA CAGGCTGAAC 
TCCACTGGCA TGTACGTGGC TAACACCAAG CCTGGCGGCT TCACCATCGA GATCAGTAAC 
AACAGCAGCA CCATGGTGAT GACGGGCATG CGGATCCAGA TTGGGACGCA GGCCATCGAG 
CGGGCCCCGT CCTACATCGA GATCTTCGGC AGGACCATGC AGCTGAACCT GAGCCGCTCC 
CGCTGGTTTG ACTTCCCCTT CACCAGAGAG GAGGCCCTGC AGGCCGACCG GAAGCTGAGC 
CTCTTCATCG GTGCCTCCGT GGATCCAGCT GGTGTCACCA TGATAGATGC TGTAAAAATT 
TATGGCAAGA CTAAAGAGCA GTTTGGCTGG CCTGATGAGC CCCCAGAAGA CTTCCCTTCT 
GCCTCAGTCA GCAACATCTG CCCTCCTAAC CTGAACCAGA GCAATGGCAC TGGAGAGAGT 
GACTCAGCCG CCCCTGCCAC AACCAGTGGC ACTGTCCTGG AGAGGCTGGT TGTGAGTTCT 
TTGGAAGCCC TGGAAAGCTG CTTTGCTGTT GGCCCAATCA TTGAGAAGGA GAGAAACAAG 
CATGCCGCTC AGGAGCTAGC CACTTTGTTG CTCTCCCTGC CAGCGCCTGC CAGTGTCCAG 
CAGCAATCAA AGAGCCTCCT GGCCAGTCTG CACAGCAGCC GCTCGGCCTA CCACAGCCAT 
AAGGACCAGG CCTTGTTGAG CAAAGCTGTG CAGTGTCTCA ACACATCCAG CAAAGAAGGC 
AAGGACTTGG ACCCCGAGGT GTTCCAGCGC CTGGTGATCA CAGCTCGCTC CATTGCCGTC 
ACACGCCCTA ACAACCTTGT GCACTTCACC GAGTCAAAGC TGCCCCAGAT GGAAACAGAA 
GGAGCAGATG AGGGGAAAGA GCCGCAGAAG CAGGAAGGAG ACGGCTGTAG TTTCATCACT 
CAGCTTGTAA ACCACTTCTG GAAACTCCAT GCATCTAAGC CCAAGAATGC CTTCCTGGCA 
CCTGCCTGCC TGCCAGGCCT TACTCATATT GAAGCTACGG TTAATGCGTT GGTAGACATT 
ATCCACGGTT ATTGTACCTG CGAGCTGGAC TGTATCAACA CAGCATCCAA GATCTACATG 
CAGATGCTGC TGTGTCCTGA CCCTGCTGTG AGCTTCTCCT GTAAACAAGC TCTGATTCGA 
GTCCTAAGGC CCAGGAACAA GCGGAGACAC GTGACGTTGC CCTCCTCCCC TCGAAGCAAC 
ACTCCAATGG GAGACAAGGA CGACGATGAT GACGATGACG CAGATGAGAA AATGCAGTCA 
TCGGGCATCC CCGATGGTGG TCACATCCGC CAGGAAAGCC AGGAACAGAG TGAGGTGGAC 
CATGGAGATT TTGAGATGGT GTCTGAGTCG ATGGTCCTGG AAACAGCTGA AAATGTCAAC 
AATGGCAACC CCTCTCCCCT GGAGGCCCTG CTGGCAGGTG CTGAAGGCTT TCCTCCCATG 
CTGGACATCC CACCTGATGC AGACGACGAG ACCATGGTTG AACTAGCCAT TGCCCTGAGC 
CTGCAGCAGG ACCAGCAAGG CAGCAGCAGC AGTGCCCTGG GCCTGCAGAG CCTGGGACTC 
TCCGGCCAGG CACCCAGCTC TTCCTCTCTG GACGCAGGAA CCCTCTCTGA CACCACAGCA 
TCAGCTCCAG CCTCAGATGA CGAGGGCAGC ACTGCAGCAA CTGACGGCTC CACCCTGCGG 
ACCTCGCCAG CTGACCATGG CGGTAGTGTG GGCTCAGAGA GCGGGGGAAG TGCAGTGGAC 
TCGGTGGCTG GCGAGCACAG TGTGTCTGGC CGGAGCAGTG CATATGGTGA CGCCACAGCT 
GAGGGGCACC CGGCTGGACC AGGGAGCGTC AGCTCTAGCA CAGGCGCCAT CAGCACCGCC 
ACTGGGCACC AGGAGGGAGA CGGCTCTGAG GGAGAAGGAG AAGGGGAAGC TGAAGGAGAT 
GTGCACACTA GCAACAGGCT ACACATGGTT CGTCTGATGC TGTTGGAAAG ATTGCTGCAG 
ACACTGCCCC AGTTACGGAA TGTCGGAGGT GTCCGGGCCA TCCCATACAT GCAGGTCATT 
CTCATGCTCA CTACAGATCT CGATGGAGAA GATGAGAAAG ACAAGGGAGC CCTGGACAAC 
CTGCTTGCAC AGCTCATCGC CGAGTTGGGC ATGGACAAAA AGGATGTCTC CAAGAAGAAC 
GAGCGCAGTG CTCTGAATGA AGTCCATTTG GTCGTGATGA GACTCCTGAG TGTCTTCATG 
TCTCGTACCA AGTCTGGATC CAAATCTTCC ATCTGTGAGT CCTCTTCCCT CATCTCCAGT 
GCCACGGCAG CAGCCCTGCT TAGCTCAGGG GCTGTGGACT ATTGCCTCCA TGTGCTCAAG 
TCTCTGCTGG AATACTGGAA GAGCCAGCAG AGTGATGAGG AGCCTGTGGC TGCCAGCCAA 
CTGCTGAAAC CACACACGAC TTCATCTCCG CCAGATATGA GCCCATTCTT TCTCCGCCAG 
TATGTGAAGG GCCATGCCGC TGATGTCTTT GAAGCCTATA CCCAGCTGCT CACAGAGATG 
GTACTGAGGC TTCCCTACCA AATCAAGAAG ATCGCAGACA CCAGCTCTCG GATTCCTCCT 
CCTGTCTTTG ATCACTCCTG GTTCTACTTC CTCTCTGAGT ACCTAATGAT CCAGCAGACC 
CCTTTTGTCC GCCGACAAGT TCGAAAACTT CTGCTCTTCA TCTGTGGATC AAAGGAGAAA 
TACCGACAGC TCCGAGACCT GCACACCCTG GACTCCCATG TGCGTGGGAT CAAGAAGCTG 
CTGGAGGAGC AGGGCATCTT CCTCCGGGCC AGTGTGGTCA CAGCCAGTTC CGGCTCCGCT 
TTGCAGTATG ACACGCTCAT CAGCCTGATG GAGCATCTGA AGGCCTGTGC AGAGATTGCC 
GCCCAGCGAA CCATCAACTG GCAGAAGTTC TGCATCAAAG ATGACTCTGT CCTGTACTTC 
CTCCTGCAAG TGAGTTTCCT GGTAGATGAG GGGGTGTCCC CCGTGCTGCT GCAGCTGCTC 
TCCTGTGCTC TGTGCGGCAG CAAAGTGCTC GCAGCCCTGG CGGCCTCCAC AGGGTCTTCC 
AGTGTGGCTT CCTCCTCAGC CCCTCCTGCA GCCAGTTCTG GACAAGCCAC AACTCAGTCC 
AAATCTTCCA CTAAAAAGAG CAAGAAAGAA GAAAAGGAAA AAGAGAAAGA GGGTGAGAGC 
TCTGGTAGCC AGGAGGACCA GCTGTGCACA GCTCTCGTGA ACCAGCTGAA CAGATTTGCA 
GACAAAGAAA CTCTGATCCA GTTTCTGCGC TGTTTCCTGT TGGAGTCCAA CTCGTCCTCA 
GTGCGCTGGC AGGCCCACTG CCTGACACTG CACATCTACA GAAACTCCAA CAAGGCTCAG 
CAGGAGCTCT TGCTAGATCT CATGTGGTCC ATCTGGCCGG AATTGCCAGC TTACGGCCGG 
AAGGCTGCCC AGTTTGTGGA CCTGCTGGGC TATTTCTCCC TGAAAACTGC ACAGACAGAG 
AAGAAGTTGA AGGAGTATTC CCAGAAGGCC GTGGAGATCC TGAGGACTCA GAACCATATC 
CTCACCAATC ACCCCAACTC CAACATCTAC AACACCTTGT CTGGCTTAGT GGAGTTTGAC 
GGCTATTACC TAGAGAGCGA CCCCTGCCTG GTGTGTAACA ATCCTGAGGT GCCGTTTTGT 
TATATCAAGC TGTCTTCCAT TAAAGTGGAC ACACGGTACA CCACCACCCA GCAGGTTGTA 
AAGCTCATTG GCAGTCACAC CATCAGCAAG GTGACAGTGA AAATTGGGGA CCTGAAGCGA 
ACCAAGATGG TGCGGACGAT CAACCTGTAC TACAACAACC GAACTGTGCA GGCCATCGTG 
GAGCTGAAAA ATAAGCCAGC TCGCTGGCAC AAAGCCAAGA AAGTTCAGCT GACTCCCGGA 
CAGACAGAAG TCAAGATTGA CCTGCCTCTG CCCATCGTGG CCTCTAACCT GATGATCGAG 
TTTGCAGACT TCTATGAGAA CTACCAGGCC TCCACAGAGA CCCTTCAATG TCCTCGCTGC 
AGTGCCTCTG TCCCCGCCAA CCCAGGCGTC TGTGGCAACT GTGGAGAGAA TGTATACCAG 
TGTCACAAGT GCAGGTCCAT CAACTATGAT GAAAAGGACC CCTTCCTCTG CAATGCTTGT 
GGCTTCTGTA AATACGCCCG CTTTGACTTC ATGCTTTATG CCAAGCCTTG CTGTGCAGTG 
GATCCCATCG AGAACGAAGA AGATCGGAAG AAGGCCGTTT CCAACATCAA CACACTTCTG 
GACAAAGCTG ACCGCGTGTA CCATCAGCTC ATGGGACATC GGCCCCAGTT GGAGAACCTG 
CTCTGCAAAG TGAATGAAGC AGCTCCAGAA AAGCCACAGG AAGACTCAGG AACAGCGGGA 
GGCATCAGCT CCACTTCAGC CAGTGTGAAT CGCTACATCC TGCAGCTGGC TCAAGAGTAT 
TGTGGAGACT GTAAGAACTC GTTCGATGAG CTCTCCAAAA TCATCCAGAA AGTTTTTGCT 
TCACGCAAAG AGCTGTTGGA ATATGATCTA CAGCAAAGAG AAGCAGCCAC CAAGTCATCC 
CGGACGTCTG TCCAGCCCAC GTTTACTGCC AGCCAGTACC GTGCTTTGTC TGTCTTAGGC 
TGTGGCCACA CCTCCTCCAC CAAGTGCTAT GGCTGTGCCT CAGCTGTCAC GGAGCATTGT 
ATTACACTAC TGAGGGCCCT GGCCACCAAT CCAGCCCTGC GACACATCCT CGTCTCCCAG 
GGCCTCATCC GAGAGCTCTT TGATTACAAC CTCCGCCGTG GCGCTGCAGC CATTCGAGAG 
GAGGTCCGCC AGCTCATGTG CCTCCTCACC AGAGACAACC CAGAGGCCAC CCAGCAAATG 
AACGACCTGA TCATTGGCAA AGTCTCCACT GCACTGAAGG GCCACTGGGC TAATCCTGAC 
CTGGCTAGCA GCCTTCAGTA TGAGATGCTG CTGCTGACAG ATTCCATCTC CAAGGAGGAC 
AGCTGCTGGG AACTCCGCTT ACGATGTGCA CTCAGCCTTT TCCTCATGGC TGTGAACATC 
AAAACGCCAG TGGTTGTTGA GAATATCACA CTCATGTGTC TGCGGATCTT ACAGAAGCTG 
ATTAAACCAC CTGCCCCAAC CAGCAAGAAG AACAAGGATG TCCCCGTGGA GGCCCTCACC 
ACAGTGAAAC CGTACTGCAA TGAGATCCAT GCCCAGGCTC AGCTGTGGCT CAAGAGAGAT 
CCCAAGGCAT CATACGAAGC CTGGAAGAAG TGCCTGCCTA TCCGAGGGGT GGATGGCAAT 
GGGAAATCCC CCAGCAAGTC AGAGCTCCAC CGTCTCTACT TGACCGAGAA GTACGTGTGG 
AGGTGGAAAC AGTTCCTGAG CCGGCGGGGG AAGAGGACCA CCCCACTTGA CCTCAAGCTG 
GGCCACAACA ACTGGCTGCG GCAGGTACTC TTTACCCCAG CAACACAGGC AGCAAGGCAG 
GCAGCCTGTA CCATCGTGGA AGCTCTTGCT ACTGTCCCCA GCCGCAAGCA GCAGGTCCTT 
GACCTCCTCA CCAGTTACCT GGACGAGCTC AGCGTTGCGG GGGAGTGTGC TGCAGAGTAC 
CTGGCTCTCT ACCAGAAGCT CATCGCCTCG TGCCACTGGA AGGTCTACCT AGCTGCACGC 
GGAGTCCTGC CCTACGTGGG CAACCTCATC ACCAAGGAAA TCGCCCGCCT GCTGGCCTTG 
GAGGAGGCCA CGCTGAGCAC AGACCTGCAG CAGGGATATG CCCTCAAGAG TCTCACAGGC 
CTTCTCTCCT CCTTTGTAGA GGTGGAATCC ATTAAGAGGC ATTTTAAGAG CCGCTTAGTG 
GGCACTGTGC TGAATGGGTA CCTGTGCTTG CGGAAGCTGG TGCTGCAGAG GACCAAGCTC 
ATCGACGAGA CCCAGGACAT GCTGCTGGAG ATGCTGGAGG ACATGACCAC AGGTACAGAG 
TCTGAAACCA AGGCCTTCAT GGCTGTGTGC ATTGAGACAG CAAAGCGCTA CAATCTGGAT 
GACTACCGGA CTCCCGTGTT CATCTTTGAG AGGCTGTGCA GCATCATCTA CCCTGAGGAG 
AATGAAGTCA CGGAGTTCTT CGTGACCCTG GAGAAGGACC CCCAGCAAGA GGACTTTCTA 
CAGGGCAGGA TGCCCGGAAA CCCATATAGC AGCAATGAAC CAGGCATTGG GCCTCTTATG 
AGGGACATAA AGAACAAGAT CTGCCAGGAC TGTGACCTAG TGGCTCTTCT GGAGGATGAC 
AGTGGGATGG AGCTCCTGGT GAACAATAAA ATTATCAGTC TGGACCTTCC CGTGGCTGAG 
GTTTACAAGA AGGTGTGGTG TGCGACCAAT GAGGGAGAGC CCATGAGGAT TGTTTATCGA 
ATGCGGGGGC TCCTGGGCGA TGCCACCGAG GAGTTTATCG AGTCCCTGGA CTCCACCACA 
GATGAAGAAG AAGATGAAGA GGAAGTGTAC AGAATGGCCG GTGTGATGGC CCAGTGTGGG 
GGTCTGCAGT GCATGTTGAA CAGACTGGCA GGAGTCAAAG ATTTTAAGCA GGGACGCCAC 
CTTCTAACAG TGCTGCTGAA GCTCTTCAGT TACTGTGTGA AGGTCAAAGT CAACCGGCAG 
CAGCTGGTCA AGCTGGAGAC GAACACTCTG AATGTCATGC TGGGGACTCT GAACCTGGCT 
CTGGTAGCCG AGCAAGAGAG CAAGGACAGC GGAGGAGCAG CTGTGGCTGA GCAGGTGCTA 
AGCATCATGG AGATCATTCT AGATGAATCC AACGCAGAGC CCCTGAGTGA GGACAAGGGC 
AACCTCCTCC TCACAGGTGA CAAGGATCAA CTGGTGATGC TCTTGGACCA GATCAACAGC 
ACCTTCGTTC GATCCAACCC TAGCGTGCTA CAGGGCTTGC TGCGTATCAT CCCATACCTG 
TCCTTCGGAG AGGTGGAGAA GATGCAGATC CTGGTGGAGC GGTTCAAGCC ATACTGCAGC 
TTCGAGAAGT ACGATGAAGA CCATAGTGGG GATGACAAAG TCTTCCTGGA TTGCTTCTGC 
AAAATTGCTG CCGGTATCAA GAACAACAGC AATGGTCATC AGCTGAAGGA TCTCATCCTG 
CAGAAGGGAA TCACCCAAAA TGCCCTGGAC TACATGAAAA AGCATATCCC CAGTGCCAAG 
AATTTGGATG CTGACATCTG GAAAAAGTTT TTGTCTCGCC CTGCTCTGCC ATTTATTTTG 
AGGCTTCTTC GGGGTCTGGC CATGCAGCAC CCTGCCACCC AGGTTCTGAT TGGAACGGAC 
TCCATCACGA GCCTGCATAA GCTGGAGCAG GTGTCCAGTG ATGAGGGCAT TGGGACCCTG 
GCTGAGAACC TGCTGGAGGC CCTGAGAGAA CACCCAGATG TGAACAAGAA GATTGACGCA 
GCCCGCAGGG AGACCCGGGC AGAGAAGAAG CGCATGGCCA TGGCCATGAG GCAGAAGGCT 
CTGGGAACTC TGGGCATGAC GACAAACGAA AAGGGTCAGG TGGTGACCAA GACTGCACTC 
CTGAAGCAGA TGGAAGAGCT GATTGAGGAG CCGGGACTCA CGTGCTGCAT CTGCAGGGAG 
GGGTACAAGT TCCAGCCCAC GAAGGTCTTA GGCATTTACA CTTTCACCAA GCGGGTAGCC 
TTGGAGGAGA TGGAGAACAA GCCCCGGAAG CAGCAGGGCT ACAGCACTGT GTCCCATTTC 
AACATTGTGC ACTACGACTG TCACCTGGCT GCCGTCAGGT TGGCTAGAGG CCGGGAAGAG 
TGGGAGAGTG CTGCCCTGCA GAACGCCAAC ACGAAGTGCA ATGGGCTCCT TCCGGTCTGG 
GGCCCCCATG TCCCTGAATC AGCTTTCGCC ACTTGCTTAG CAAGGCACAA CACGTACCTC 
CAGGAGTGTA CCGGCCAGCG GGAGCCCACG TACCAGCTCA ACATCCATGA CATCAAACTG 
CTCTTCCTGC GCTTCGCCAT GGAGCAGTCA TTCAGTGCAG ACACCGGTGG GGGCGGCCGT 
GAGAGCAACA TCCACCTGAT CCCATACATC ATTCACACTG TGCTTTACGT CCTGAACACG 
ACCCGAGCAA CGTCCCGGGA GGAGAAGAAC CTCCAAGGCT TCCTGGAACA GCCCAAAGAG 
AAGTGGACAG AGAGTGCCTT CGATGTAGAT GGGCCCCACT ACTTCACCAT CCTAGCCCTG 
CACGTCCTCC CCCCTGAACA GTGGAAAGCC ATTCGAGTAG AGATCCTACG CAGGCTGCTG 
GTGGCCTCAC ATGCCCGGGC AGTGGCTCCA GGAGGAGCCA CCAGGTTGAC AGACAAGGCG 
GTGAAGGACT ATTCTGCGTA CCGTTCTTCC CTGCTCTTCT GGGCTCTCGT CGATCTCATT 
TATAACATGT TTAAGAAGGT GCCCACTAGT AACACTGAGG GCGGCTGGTC CTGCTCTCTG 
GCCGAGTACA TCCGCCACAA TGACATGCCC ATCTACGAAG CTGCCGACAA AGCCCTGAAA 
ACCTTCCAGG AGGAGTTCAT GCCAGTGGAG ACCTTCTCGG AGTTCCTCGA CGCAGCAGGT 
CTTCTATCAG AAATCACCGA TCCAGAGAGC TTCCTAAAGG ACCTGTTGAA CTCAGTCCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153791.1
CDS	29..15571
Translation	MATSGGEEAAAAAPAPGAPATGQDTTPGWEVAVRPLLSASYSAFEMKELPQLVASVIESESEILHHEKQYEPFYSSFVALSTHYITTVCSLIPRNQLQSVAAACKVLIEFSLLRLENPDEACAVSQKHLILLIKGLCTGCSRLDRTEIITFTAMMKSAKLPQTVKTLSDVEDQKELASPVSPELRQKEVQMNFLNQLTSVFNPRTVPSPPISPQALVEGENDEQSSPDQVSAAKTKSVFIAQNVASLQELGGSEKLLRVCLNLPYFLRYINRFQDAVVANSFFIMPATVADATAVRNGFHSLVIDVTMALDTLSLPVLEPLNPSRLQDVTVLSLSCLYAGVSVATCMAILHVGSAQQVRTGSTSSKEDDYESDAATIVQKCLEIYDMIGQAISSSRRAGGEHFQNFQLLGAWCLLNSLFLILNLSPTALADKGKEKDPLAALRVRDILSRTKEGVGSPKLGPGKGHQGFGVLSVILANHAIKLLASLFQDLQVEALHKGWETDGPPAVLSIMAQSTSTQRIQRLIDSVPLTNLLLTLLSTSYRKACVLQRQRKGSMSSDASASTDSNTYYEDDFSSTEEDSSQDDDSEPILGQWFEETISPSKEKAAPPPPPPPPPLESSPRVKSPNKQASGEKGNILASRKDPELFSGLASNILNFITTSMLNSRNSFIRSYLSASLSEHHMATLASIIKEVDKDGLKGSSDEDFAAALYHFNHSLVTSDLQSPNLQNTLLQQLGVAPFSEGPWPLYIHPQGLSVLSRLLLIWQHKAGAQGDPDVPECLKVWDRFLTTMKQNALQGVVPSETEDLNVEHLQLLLLIFHSFSEKGRRAILTMLVQSIQELSVNMEVQMRTAPLILARLLLIFDYLLHQYSKAPVYLFEQVQHNLLSPPFGWASGSQDSSSRRANTPLYHGFKEVEENWSKHFSSDAAPQPRFYCVLSTEASEEDLNRLDSEACEVLFSKPVKYDELYSSLTTLLAAGSQLDTTRRKEKKNVTALEACALQYYFLILWRILGILPPSKTYMNQLAMNSPEMSECDILHTLRWSSRLRISSYVSWIKDHLIKQGMKPEHAGSLIELAASKCSSVKYDVEIVEEYFARQISSFCSIDCTAVLQLHEIPSLQSIYTLDAAVSKVQVSLDEHFSKMAAETDPHKSSEITKNLLPATLQLIDTYASFTRAYLLQNLNEEGSTEKPSQEKLHGFAAVLAIGSSRCKANTLGPTLVQNLPSSVQSVCESWNNINTNEFPNIGSWRNAFANDTIPSESYISAVQAAHLGTLCGQSLPLAASLKHTLLSLVRLTGDLIVWSDEMNPAQVIRTLLPLLLESSTESAAEISSNSLERILGPAESDEFLARVYEKLITGCYNILANHADPNSGLDESILEECLQYLEKQLESSQARKAMEEFFSDGGELVQIMMATANEDLSAKFCNRVLKFFTKLFQLTEKSPNPSLLHLCGSLAQLACVEPVRLQAWLTRMTTSPPKDSDQLEVIQENRQLLQLLTTYIVRENSQVGEGVCAVLLGTLTPMATDMLANGDGTGFPELMVVMATLASAGQGAGHLQLHNAAVDWLGRCKKYLSQKNVVEKLNANVMHGKHVMVLECTCHIMSYLADVTNALSQSNGQGPSHLSVDGEERAIEVDSDWVEELAVEEEDSQAEDSDEDSLCNKLCTFTITQKEFMNQHWYHCHTCKMVDGVGVCTVCAKVCHKDHEISYAKYGSFFCDCGAKEDGSCLALVKRTPSSGMSSTMKESAFQSEPRVSESLVRHASTSPADKAKVTISDGKVTDEEKPKKSSLCRTVEGCREELQNQANFSFAPLVLDMLSFLMDAIQTNFQQASAVGSSSRAQQALSELHTVDKGVEMTDQLMVPTLGSQEGAFENVRMNYSGDQGQTIRQLISAHVLRRVAMCVLSSPHGRRQHLAVSHEKGKITVLQLSALLKQADSSKRKLTLTRLASAPVPFTVLSLTGNPCKEDYLAVCGLKDCHVLTFSSSGSVSDHLVLHPQLATGNFIIKAVWLPGSQTELAIVTADFVKIYDLSIDALSPTFYFLLPSSKIRDVTFLFNEEGKNIIVIMSSAGYMYTQLMEEASSAQQGPFYVTNVLEINHEDLKDSNSQVAGGGVSVYYSHVLQMLFFSYSQGRSFAATVSRSTLEVLQLFPINIKSSNGGSKTSPALCQWSEVMNHPGLVCCVQQTTGVPLVVMVKPGTFLIQEIKTLPAKAKIQDMVAIRHTACNEQQRTTMILLCEDGSLRIYMANVENTSYWLQPSLQPSSVISIMKPVRKRKTATITARTSSQVTFPIDFFEHNQQLTDVEFGGNDLLQVYNAQQIKHRLNSTGMYVANTKPGGFTIEISNNSSTMVMTGMRIQIGTQAIERAPSYIEIFGRTMQLNLSRSRWFDFPFTREEALQADRKLSLFIGASVDPAGVTMIDAVKIYGKTKEQFGWPDEPPEDFPSASVSNICPPNLNQSNGTGESDSAAPATTSGTVLERLVVSSLEALESCFAVGPIIEKERNKHAAQELATLLLSLPAPASVQQQSKSLLASLHSSRSAYHSHKDQALLSKAVQCLNTSSKEGKDLDPEVFQRLVITARSIAVTRPNNLVHFTESKLPQMETEGADEGKEPQKQEGDGCSFITQLVNHFWKLHASKPKNAFLAPACLPGLTHIEATVNALVDIIHGYCTCELDCINTASKIYMQMLLCPDPAVSFSCKQALIRVLRPRNKRRHVTLPSSPRSNTPMGDKDDDDDDDADEKMQSSGIPDGGHIRQESQEQSEVDHGDFEMVSESMVLETAENVNNGNPSPLEALLAGAEGFPPMLDIPPDADDETMVELAIALSLQQDQQGSSSSALGLQSLGLSGQAPSSSSLDAGTLSDTTASAPASDDEGSTAATDGSTLRTSPADHGGSVGSESGGSAVDSVAGEHSVSGRSSAYGDATAEGHPAGPGSVSSSTGAISTATGHQEGDGSEGEGEGEAEGDVHTSNRLHMVRLMLLERLLQTLPQLRNVGGVRAIPYMQVILMLTTDLDGEDEKDKGALDNLLAQLIAELGMDKKDVSKKNERSALNEVHLVVMRLLSVFMSRTKSGSKSSICESSSLISSATAAALLSSGAVDYCLHVLKSLLEYWKSQQSDEEPVAASQLLKPHTTSSPPDMSPFFLRQYVKGHAADVFEAYTQLLTEMVLRLPYQIKKIADTSSRIPPPVFDHSWFYFLSEYLMIQQTPFVRRQVRKLLLFICGSKEKYRQLRDLHTLDSHVRGIKKLLEEQGIFLRASVVTASSGSALQYDTLISLMEHLKACAEIAAQRTINWQKFCIKDDSVLYFLLQVSFLVDEGVSPVLLQLLSCALCGSKVLAALAASTGSSSVASSSAPPAASSGQATTQSKSSTKKSKKEEKEKEKEGESSGSQEDQLCTALVNQLNRFADKETLIQFLRCFLLESNSSSVRWQAHCLTLHIYRNSNKAQQELLLDLMWSIWPELPAYGRKAAQFVDLLGYFSLKTAQTEKKLKEYSQKAVEILRTQNHILTNHPNSNIYNTLSGLVEFDGYYLESDPCLVCNNPEVPFCYIKLSSIKVDTRYTTTQQVVKLIGSHTISKVTVKIGDLKRTKMVRTINLYYNNRTVQAIVELKNKPARWHKAKKVQLTPGQTEVKIDLPLPIVASNLMIEFADFYENYQASTETLQCPRCSASVPANPGVCGNCGENVYQCHKCRSINYDEKDPFLCNACGFCKYARFDFMLYAKPCCAVDPIENEEDRKKAVSNINTLLDKADRVYHQLMGHRPQLENLLCKVNEAAPEKPQEDSGTAGGISSTSASVNRYILQLAQEYCGDCKNSFDELSKIIQKVFASRKELLEYDLQQREAATKSSRTSVQPTFTASQYRALSVLGCGHTSSTKCYGCASAVTEHCITLLRALATNPALRHILVSQGLIRELFDYNLRRGAAAIREEVRQLMCLLTRDNPEATQQMNDLIIGKVSTALKGHWANPDLASSLQYEMLLLTDSISKEDSCWELRLRCALSLFLMAVNIKTPVVVENITLMCLRILQKLIKPPAPTSKKNKDVPVEALTTVKPYCNEIHAQAQLWLKRDPKASYEAWKKCLPIRGVDGNGKSPSKSELHRLYLTEKYVWRWKQFLSRRGKRTTPLDLKLGHNNWLRQVLFTPATQAARQAACTIVEALATVPSRKQQVLDLLTSYLDELSVAGECAAEYLALYQKLIASCHWKVYLAARGVLPYVGNLITKEIARLLALEEATLSTDLQQGYALKSLTGLLSSFVEVESIKRHFKSRLVGTVLNGYLCLRKLVLQRTKLIDETQDMLLEMLEDMTTGTESETKAFMAVCIETAKRYNLDDYRTPVFIFERLCSIIYPEENEVTEFFVTLEKDPQQEDFLQGRMPGNPYSSNEPGIGPLMRDIKNKICQDCDLVALLEDDSGMELLVNNKIISLDLPVAEVYKKVWCATNEGEPMRIVYRMRGLLGDATEEFIESLDSTTDEEEDEEEVYRMAGVMAQCGGLQCMLNRLAGVKDFKQGRHLLTVLLKLFSYCVKVKVNRQQLVKLETNTLNVMLGTLNLALVAEQESKDSGGAAVAEQVLSIMEIILDESNAEPLSEDKGNLLLTGDKDQLVMLLDQINSTFVRSNPSVLQGLLRIIPYLSFGEVEKMQILVERFKPYCSFEKYDEDHSGDDKVFLDCFCKIAAGIKNNSNGHQLKDLILQKGITQNALDYMKKHIPSAKNLDADIWKKFLSRPALPFILRLLRGLAMQHPATQVLIGTDSITSLHKLEQVSSDEGIGTLAENLLEALREHPDVNKKIDAARRETRAEKKRMAMAMRQKALGTLGMTTNEKGQVVTKTALLKQMEELIEEPGLTCCICREGYKFQPTKVLGIYTFTKRVALEEMENKPRKQQGYSTVSHFNIVHYDCHLAAVRLARGREEWESAALQNANTKCNGLLPVWGPHVPESAFATCLARHNTYLQECTGQREPTYQLNIHDIKLLFLRFAMEQSFSADTGGGGRESNIHLIPYIIHTVLYVLNTTRATSREEKNLQGFLEQPKEKWTESAFDVDGPHYFTILALHVLPPEQWKAIRVEILRRLLVASHARAVAPGGATRLTDKAVKDYSAYRSSLLFWALVDLIYNMFKKVPTSNTEGGWSCSLAEYIRHNDMPIYEAADKALKTFQEEFMPVETFSEFLDAAGLLSEITDPESFLKDLLNSVP

Target ORF information:

RefSeq Version	NM_001160319.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3 component n-recognin 4 (Ubr4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160319.1

ORF Insert Sequence:

ATGGCGACGA GCGGCGGCGA AGAGGCGGCG GCAGCAGCTC CAGCGCCCGG AGCCCCGGCG 
ACCGGGCAGG ACACGACCCC GGGCTGGGAG GTGGCGGTGC GGCCCCTCCT GTCCGCGTCC 
TACTCCGCCT TCGAGATGAA GGAGTTGCCG CAGCTGGTGG CCTCAGTCAT CGAGAGTGAG 
TCAGAAATCC TGCACCACGA GAAGCAGTAC GAGCCGTTCT ACTCCTCCTT TGTCGCACTG 
TCCACGCACT ACATCACCAC GGTCTGCAGC CTCATTCCCC GGAACCAGCT TCAGTCGGTG 
GCAGCTGCCT GTAAAGTTCT CATTGAGTTT TCCCTCCTGC GTCTGGAGAA CCCTGACGAG 
GCATGTGCTG TATCCCAGAA ACACTTGATT CTGCTGATAA AAGGCCTGTG CACTGGCTGT 
AGCCGACTAG ACAGAACAGA GATTATCACC TTTACAGCAA TGATGAAGTC CGCCAAGCTT 
CCACAGACAG TGAAGACGCT TTCAGACGTG GAAGATCAGA AAGAGCTGGC CTCCCCAGTA 
AGCCCCGAGC TCAGGCAGAA GGAGGTACAG ATGAACTTTT TGAACCAGCT GACCTCAGTT 
TTTAACCCTA GAACTGTACC ATCTCCTCCC ATCAGTCCAC AGGCTCTGGT GGAAGGAGAA 
AATGATGAGC AGTCATCTCC AGATCAGGTC TCTGCTGCCA AGACCAAGAG TGTGTTCATA 
GCTCAGAATG TGGCTAGCCT TCAAGAGCTT GGGGGTTCCG AGAAGCTACT CCGAGTATGC 
CTGAACCTGC CCTATTTCCT GCGCTACATC AATCGGTTTC AGGATGCTGT GGTGGCCAAT 
TCCTTCTTCA TCATGCCAGC AACAGTAGCT GATGCCACCG CTGTTCGAAA TGGTTTTCAC 
TCACTGGTGA TTGATGTAAC CATGGCCTTG GATACCCTTT CCCTGCCTGT GCTGGAACCC 
CTCAATCCTT CTCGTCTGCA AGATGTGACA GTGCTCAGCC TCAGCTGTCT GTATGCAGGT 
GTGAGTGTAG CCACGTGTAT GGCCATCCTT CATGTGGGTA GTGCCCAGCA AGTGCGGACA 
GGGTCCACAA GCTCCAAAGA AGATGACTAC GAAAGTGATG CAGCCACGAT TGTTCAGAAA 
TGTCTTGAGA TCTACGACAT GATTGGACAG GCCATCAGCA GCTCCCGCCG CGCAGGTGGT 
GAGCACTTCC AGAACTTCCA GCTGTTGGGC GCCTGGTGCC TGCTTAATAG CCTCTTCCTC 
ATACTGAACC TCAGCCCCAC AGCCCTGGCT GACAAGGGGA AAGAGAAGGA CCCATTGGCT 
GCCCTTCGCG TCAGAGACAT CCTTTCTCGT ACAAAGGAGG GAGTGGGCTC TCCCAAACTG 
GGGCCTGGCA AAGGGCATCA GGGATTTGGG GTACTCTCGG TGATACTGGC AAACCATGCC 
ATCAAGCTGC TGGCGTCTCT CTTTCAAGAC TTGCAGGTGG AGGCACTCCA CAAGGGCTGG 
GAGACAGATG GCCCCCCTGC AGTCCTGAGT ATTATGGCCC AGAGCACCTC CACACAAAGG 
ATTCAACGGC TGATTGACTC TGTCCCACTG ACAAACCTGC TCTTGACTTT ACTTTCAACT 
TCATACAGAA AGGCGTGTGT CCTGCAGCGG CAGAGGAAGG GCTCCATGAG CAGTGATGCC 
AGCGCCTCCA CCGACTCCAA CACGTACTAT GAGGATGACT TCAGTAGCAC AGAGGAGGAC 
AGCAGCCAAG ATGATGACAG TGAGCCTATT TTGGGGCAGT GGTTTGAGGA GACCATCTCT 
CCCAGTAAAG AAAAAGCTGC GCCTCCACCT CCTCCACCAC CCCCTCCCCT GGAGAGCTCT 
CCTCGGGTTA AAAGCCCCAA TAAGCAGGCA TCTGGTGAGA AGGGCAACAT CTTGGCCAGC 
CGCAAAGACC CTGAGTTGTT CTCAGGCCTG GCTTCCAACA TCCTGAACTT CATCACGACT 
TCCATGCTGA ACTCTCGAAA CAGTTTCATC CGGAGCTATC TGAGCGCCTC TCTCTCAGAG 
CACCACATGG CCACTCTGGC CAGCATCATT AAGGAGGTGG ACAAAGATGG ACTCAAGGGG 
TCATCAGATG AGGACTTTGC TGCTGCTCTC TATCACTTCA ACCACTCGCT GGTGACTTCT 
GACCTACAGT CCCCAAACCT GCAGAATACA CTGCTCCAGC AGCTGGGAGT TGCTCCTTTC 
TCCGAAGGCC CTTGGCCCCT GTACATTCAC CCCCAAGGCC TCTCTGTGCT TTCTCGCCTC 
CTGCTTATCT GGCAGCATAA AGCTGGAGCC CAAGGTGACC CTGATGTCCC TGAATGCCTG 
AAAGTGTGGG ACAGGTTTTT AACTACCATG AAGCAGAATG CACTGCAGGG GGTGGTGCCC 
AGTGAGACAG AAGACCTGAA CGTTGAGCAC CTGCAGCTGC TGCTGCTCAT CTTCCACAGC 
TTCTCTGAGA AGGGCCGCAG AGCCATCTTG ACCATGCTTG TGCAGAGCAT CCAGGAGCTG 
AGTGTTAACA TGGAGGTGCA GATGCGCACT GCACCGCTGA TCCTGGCCCG CCTCCTCTTG 
ATCTTTGACT ACCTACTTCA TCAGTACTCC AAAGCCCCCG TGTATCTGTT TGAGCAGGTG 
CAGCACAATC TGCTAAGTCC CCCTTTTGGG TGGGCAAGTG GATCCCAAGA CAGCAGCAGC 
CGCCGAGCCA ACACTCCTCT GTATCATGGC TTCAAGGAAG TAGAAGAAAA TTGGTCCAAA 
CATTTTTCAT CAGATGCGGC CCCACAACCC AGGTTCTACT GCGTCCTGTC CACAGAGGCC 
TCTGAGGAGG ATTTGAACCG GCTGGATTCT GAGGCATGTG AAGTTCTTTT CTCCAAGCCT 
GTCAAGTACG ATGAACTTTA TTCCTCACTG ACTACCCTGC TCGCGGCTGG ATCTCAGCTG 
GATACAACCA GGAGGAAGGA AAAGAAGAAC GTGACAGCCC TGGAGGCCTG CGCCCTGCAG 
TATTACTTCT TGATTCTCTG GAGGATCTTA GGAATTTTGC CACCTTCAAA GACTTACATG 
AACCAGCTGG CCATGAATTC ACCTGAGATG AGTGAATGTG ACATCTTACA CACTCTGCGA 
TGGTCGTCTC GCCTCCGGAT CAGTTCCTAT GTCAGCTGGA TAAAGGATCA CCTTATCAAA 
CAAGGCATGA AGCCCGAGCA TGCGGGCTCT CTCATAGAAC TGGCTGCCAG CAAGTGTAGT 
TCAGTGAAGT ACGATGTTGA GATTGTAGAG GAGTACTTTG CCCGACAGAT CTCGTCCTTC 
TGCTCCATTG ACTGCACTGC CGTCCTCCAG CTGCATGAGA TCCCCAGTCT GCAGTCCATC 
TACACCCTGG ATGCCGCTGT CTCGAAGGTC CAGGTCTCTC TGGATGAGCA CTTCTCTAAG 
ATGGCCGCAG AGACCGACCC CCATAAGTCA TCTGAGATCA CCAAGAACCT CCTTCCAGCT 
ACGCTGCAGC TCATTGACAC CTATGCCTCG TTCACCAGAG CCTATCTGCT GCAGAACCTT 
AATGAAGAGG GGTCCACTGA GAAGCCCTCC CAGGAGAAGC TGCATGGCTT TGCAGCTGTC 
TTGGCTATCG GCTCCAGTAG GTGCAAGGCA AACACACTGG GACCAACTCT AGTGCAGAAT 
TTACCGTCAT CAGTGCAGTC TGTCTGTGAG TCCTGGAATA ACATCAATAC CAATGAGTTT 
CCCAACATTG GATCCTGGCG CAATGCATTT GCCAACGACA CCATCCCGTC CGAGAGTTAC 
ATCAGTGCCG TGCAGGCTGC TCACCTGGGG ACTCTGTGTG GCCAGAGTCT GCCCCTGGCG 
GCTTCCCTGA AACATACCCT CCTCTCCCTG GTCAGGTTGA CTGGGGATCT GATTGTGTGG 
TCAGATGAGA TGAACCCAGC ACAGGTGATC CGGACTCTGT TACCGCTTCT CTTGGAGTCA 
AGCACAGAGA GCGCTGCTGA GATCAGCAGT AACTCTCTGG AGCGCATCTT GGGACCTGCG 
GAGTCTGATG AGTTCCTGGC TCGTGTGTAT GAGAAGCTGA TCACGGGATG TTACAACATT 
CTGGCCAACC ATGCAGATCC CAACAGTGGG CTGGATGAGT CCATCCTGGA GGAGTGTTTA 
CAGTATCTGG AGAAGCAGCT GGAGAGCAGC CAGGCCCGGA AGGCCATGGA GGAGTTCTTC 
TCTGATGGTG GGGAGCTGGT GCAGATCATG ATGGCCACAG CCAATGAGGA CCTCTCTGCT 
AAATTCTGTA ACCGAGTCTT GAAATTCTTC ACCAAACTCT TCCAGCTGAC TGAGAAGAGC 
CCTAACCCGA GCCTCCTTCA CCTCTGTGGC TCTCTCGCCC AGCTTGCGTG TGTGGAGCCG 
GTACGCCTGC AGGCCTGGCT CACCCGCATG ACAACATCGC CCCCGAAGGA TTCTGACCAG 
CTGGAGGTCA TTCAGGAGAA CCGGCAGCTG CTGCAGTTAC TGACCACGTA CATCGTTCGG 
GAGAACAGCC AAGTTGGAGA AGGTGTGTGT GCTGTGCTTC TGGGCACCCT GACCCCCATG 
GCAACAGATA TGCTGGCGAA CGGTGATGGG ACCGGCTTCC CTGAGCTCAT GGTTGTGATG 
GCCACTCTAG CCAGTGCCGG GCAAGGAGCT GGCCACCTTC AGCTTCACAA TGCTGCAGTG 
GACTGGCTGG GCAGATGCAA GAAATACCTG TCACAGAAGA ACGTGGTTGA GAAGCTGAAT 
GCCAATGTAA TGCATGGAAA GCATGTGATG GTCTTGGAGT GCACATGTCA TATTATGTCT 
TACCTGGCCG ATGTCACAAA TGCCCTGAGC CAGAGTAATG GTCAAGGCCC AAGCCATCTG 
TCAGTGGATG GGGAAGAGCG TGCCATTGAG GTGGATTCAG ACTGGGTGGA AGAGTTAGCG 
GTGGAGGAGG AAGACTCCCA GGCTGAGGAC TCTGATGAAG ATTCTCTTTG CAATAAGCTG 
TGTACCTTCA CCATCACACA GAAGGAGTTT ATGAACCAGC ATTGGTACCA CTGTCATACC 
TGTAAGATGG TGGATGGGGT GGGTGTGTGC ACAGTGTGTG CAAAGGTATG CCATAAGGAC 
CATGAGATTT CCTACGCCAA GTATGGTTCC TTTTTCTGTG ACTGTGGAGC CAAGGAAGAT 
GGCAGCTGCT TGGCCCTGGT GAAGCGAACT CCTAGCAGTG GCATGAGCTC TACCATGAAG 
GAGTCAGCAT TTCAGAGCGA ACCTAGGGTT TCCGAAAGTC TGGTGCGCCA TGCCAGCACC 
TCTCCTGCTG ACAAGGCCAA GGTCACCATC AGTGATGGGA AGGTTACAGA TGAAGAGAAG 
CCCAAGAAGA GCAGCCTGTG CCGTACAGTT GAGGGGTGTC GTGAGGAGCT GCAGAATCAG 
GCCAATTTCT CCTTCGCTCC TCTCGTGTTA GACATGCTCA GTTTCCTCAT GGATGCCATT 
CAGACCAACT TTCAGCAGGC TTCAGCCGTG GGGAGCAGCA GCAGGGCCCA GCAGGCCCTC 
AGTGAACTGC ACACAGTGGA CAAGGGTGTG GAGATGACAG ACCAGCTGAT GGTTCCCACC 
TTAGGCTCAC AGGAAGGTGC CTTTGAGAAT GTTCGGATGA ATTACAGCGG AGACCAGGGC 
CAGACTATAC GGCAGCTGAT CAGTGCCCAC GTGCTGAGAC GGGTGGCTAT GTGTGTGCTT 
TCTTCCCCCC ATGGGCGCCG CCAGCATTTG GCTGTTAGCC ATGAGAAGGG CAAGATCACA 
GTGCTGCAGC TCTCAGCGCT CCTGAAGCAA GCAGATTCCA GTAAGAGGAA GCTGACGCTA 
ACCCGCTTGG CTTCTGCCCC AGTTCCCTTC ACAGTGCTGA GTCTTACTGG AAACCCCTGC 
AAGGAGGACT ACCTGGCAGT GTGTGGGCTG AAGGACTGCC ACGTGCTCAC CTTCAGCAGC 
TCAGGCTCTG TCTCCGATCA CTTAGTGTTA CATCCCCAGT TGGCCACAGG GAACTTCATC 
ATCAAAGCTG TGTGGCTCCC TGGTTCACAG ACTGAGCTAG CCATCGTCAC CGCAGACTTC 
GTCAAGATTT ACGACCTGTC TATTGATGCC TTGAGTCCAA CCTTCTACTT CCTCCTGCCG 
AGCTCAAAGA TAAGAGACGT TACCTTCCTT TTCAACGAGG AAGGCAAGAA CATCATTGTC 
ATCATGTCCT CTGCTGGGTA CATGTACACA CAACTCATGG AGGAGGCCAG CAGTGCCCAG 
CAGGGCCCCT TCTATGTCAC TAATGTGCTG GAAATAAATC ACGAGGACCT GAAGGACAGT 
AACAGCCAGG TGGCAGGCGG TGGCGTGTCT GTGTACTACT CACACGTGTT GCAGATGCTT 
TTCTTCAGCT ACAGTCAGGG CAGGTCCTTT GCAGCCACTG TCAGCAGGAG CACCCTGGAG 
GTGTTGCAGC TCTTCCCCAT CAACATCAAA AGCTCCAATG GTGGCAGTAA GACTTCTCCT 
GCCCTTTGCC AGTGGTCCGA GGTGATGAAC CACCCTGGCT TGGTGTGTTG TGTTCAGCAG 
ACTACTGGCG TGCCTCTGGT GGTCATGGTG AAGCCAGGCA CTTTCCTCAT CCAGGAGATT 
AAGACTCTTC CCGCCAAAGC AAAGATCCAA GACATGGTTG CCATTAGGCA CACTGCCTGC 
AATGAGCAGC AACGTACAAC CATGATCCTG CTGTGCGAGG ACGGCAGCCT GCGGATTTAC 
ATGGCCAATG TAGAGAACAC CTCTTACTGG CTCCAGCCGT CTCTGCAGCC CAGCAGTGTC 
ATCAGCATCA TGAAACCTGT GCGGAAGCGC AAAACAGCTA CCATCACAGC CCGCACATCC 
AGCCAGGTCA CCTTCCCCAT TGACTTTTTT GAACACAACC AGCAGCTAAC AGATGTGGAG 
TTTGGCGGTA ATGACCTCTT GCAAGTCTAC AATGCACAGC AGATAAAGCA CAGGCTGAAC 
TCCACTGGCA TGTACGTGGC TAACACCAAG CCTGGCGGCT TCACCATCGA GATCAGTAAC 
AACAGCAGCA CCATGGTGAT GACGGGCATG CGGATCCAGA TTGGGACGCA GGCCATCGAG 
CGGGCCCCGT CCTACATCGA GATCTTCGGC AGGACCATGC AGCTGAACCT GAGCCGCTCC 
CGCTGGTTTG ACTTCCCCTT CACCAGAGAG GAGGCCCTGC AGGCCGACCG GAAGCTGAGC 
CTCTTCATCG GTGCCTCCGT GGATCCAGCT GGTGTCACCA TGATAGATGC TGTAAAAATT 
TATGGCAAGA CTAAAGAGCA GTTTGGCTGG CCTGATGAGC CCCCAGAAGA CTTCCCTTCT 
GCCTCAGTCA GCAACATCTG CCCTCCTAAC CTGAACCAGA GCAATGGCAC TGGAGAGAGT 
GACTCAGCCG CCCCTGCCAC AACCAGTGGC ACTGTCCTGG AGAGGCTGGT TGTGAGTTCT 
TTGGAAGCCC TGGAAAGCTG CTTTGCTGTT GGCCCAATCA TTGAGAAGGA GAGAAACAAG 
CATGCCGCTC AGGAGCTAGC CACTTTGTTG CTCTCCCTGC CAGCGCCTGC CAGTGTCCAG 
CAGCAATCAA AGAGCCTCCT GGCCAGTCTG CACAGCAGCC GCTCGGCCTA CCACAGCCAT 
AAGGACCAGG CCTTGTTGAG CAAAGCTGTG CAGTGTCTCA ACACATCCAG CAAAGAAGGC 
AAGGACTTGG ACCCCGAGGT GTTCCAGCGC CTGGTGATCA CAGCTCGCTC CATTGCCGTC 
ACACGCCCTA ACAACCTTGT GCACTTCACC GAGTCAAAGC TGCCCCAGAT GGAAACAGAA 
GGAGCAGATG AGGGGAAAGA GCCGCAGAAG CAGGAAGGAG ACGGCTGTAG TTTCATCACT 
CAGCTTGTAA ACCACTTCTG GAAACTCCAT GCATCTAAGC CCAAGAATGC CTTCCTGGCA 
CCTGCCTGCC TGCCAGGCCT TACTCATATT GAAGCTACGG TTAATGCGTT GGTAGACATT 
ATCCACGGTT ATTGTACCTG CGAGCTGGAC TGTATCAACA CAGCATCCAA GATCTACATG 
CAGATGCTGC TGTGTCCTGA CCCTGCTGTG AGCTTCTCCT GTAAACAAGC TCTGATTCGA 
GTCCTAAGGC CCAGGAACAA GCGGAGACAC GTGACGTTGC CCTCCTCCCC TCGAAGCAAC 
ACTCCAATGG GAGACAAGGA CGACGATGAT GACGATGACG CAGATGAGAA AATGCAGTCA 
TCGGGCATCC CCGATGGTGG TCACATCCGC CAGGAAAGCC AGGAACAGAG TGAGGTGGAC 
CATGGAGATT TTGAGATGGT GTCTGAGTCG ATGGTCCTGG AAACAGCTGA AAATGTCAAC 
AATGGCAACC CCTCTCCCCT GGAGGCCCTG CTGGCAGGTG CTGAAGGCTT TCCTCCCATG 
CTGGACATCC CACCTGATGC AGACGACGAG ACCATGGTTG AACTAGCCAT TGCCCTGAGC 
CTGCAGCAGG ACCAGCAAGG CAGCAGCAGC AGTGCCCTGG GCCTGCAGAG CCTGGGACTC 
TCCGGCCAGG CACCCAGCTC TTCCTCTCTG GACGCAGGAA CCCTCTCTGA CACCACAGCA 
TCAGCTCCAG CCTCAGATGA CGAGGGCAGC ACTGCAGCAA CTGACGGCTC CACCCTGCGG 
ACCTCGCCAG CTGACCATGG CGGTAGTGTG GGCTCAGAGA GCGGGGGAAG TGCAGTGGAC 
TCGGTGGCTG GCGAGCACAG TGTGTCTGGC CGGAGCAGTG CATATGGTGA CGCCACAGCT 
GAGGGGCACC CGGCTGGACC AGGGAGCGTC AGCTCTAGCA CAGGCGCCAT CAGCACCGCC 
ACTGGGCACC AGGAGGGAGA CGGCTCTGAG GGAGAAGGAG AAGGGGAAGC TGAAGGAGAT 
GTGCACACTA GCAACAGGCT ACACATGGTT CGTCTGATGC TGTTGGAAAG ATTGCTGCAG 
ACACTGCCCC AGTTACGGAA TGTCGGAGGT GTCCGGGCCA TCCCATACAT GCAGGTCATT 
CTCATGCTCA CTACAGATCT CGATGGAGAA GATGAGAAAG ACAAGGGAGC CCTGGACAAC 
CTGCTTGCAC AGCTCATCGC CGAGTTGGGC ATGGACAAAA AGGATGTCTC CAAGAAGAAC 
GAGCGCAGTG CTCTGAATGA AGTCCATTTG GTCGTGATGA GACTCCTGAG TGTCTTCATG 
TCTCGTACCA AGTCTGGATC CAAATCTTCC ATCTGTGAGT CCTCTTCCCT CATCTCCAGT 
GCCACGGCAG CAGCCCTGCT TAGCTCAGGG GCTGTGGACT ATTGCCTCCA TGTGCTCAAG 
TCTCTGCTGG AATACTGGAA GAGCCAGCAG AGTGATGAGG AGCCTGTGGC TGCCAGCCAA 
CTGCTGAAAC CACACACGAC TTCATCTCCG CCAGATATGA GCCCATTCTT TCTCCGCCAG 
TATGTGAAGG GCCATGCCGC TGATGTCTTT GAAGCCTATA CCCAGCTGCT CACAGAGATG 
GTACTGAGGC TTCCCTACCA AATCAAGAAG ATCGCAGACA CCAGCTCTCG GATTCCTCCT 
CCTGTCTTTG ATCACTCCTG GTTCTACTTC CTCTCTGAGT ACCTAATGAT CCAGCAGACC 
CCTTTTGTCC GCCGACAAGT TCGAAAACTT CTGCTCTTCA TCTGTGGATC AAAGGAGAAA 
TACCGACAGC TCCGAGACCT GCACACCCTG GACTCCCATG TGCGTGGGAT CAAGAAGCTG 
CTGGAGGAGC AGGGCATCTT CCTCCGGGCC AGTGTGGTCA CAGCCAGTTC CGGCTCCGCT 
TTGCAGTATG ACACGCTCAT CAGCCTGATG GAGCATCTGA AGGCCTGTGC AGAGATTGCC 
GCCCAGCGAA CCATCAACTG GCAGAAGTTC TGCATCAAAG ATGACTCTGT CCTGTACTTC 
CTCCTGCAAG TGAGTTTCCT GGTAGATGAG GGGGTGTCCC CCGTGCTGCT GCAGCTGCTC 
TCCTGTGCTC TGTGCGGCAG CAAAGTGCTC GCAGCCCTGG CGGCCTCCAC AGGGTCTTCC 
AGTGTGGCTT CCTCCTCAGC CCCTCCTGCA GCCAGTTCTG GACAAGCCAC AACTCAGTCC 
AAATCTTCCA CTAAAAAGAG CAAGAAAGAA GAAAAGGAAA AAGAGAAAGA GGGTGAGAGC 
TCTGGTAGCC AGGAGGACCA GCTGTGCACA GCTCTCGTGA ACCAGCTGAA CAGATTTGCA 
GACAAAGAAA CTCTGATCCA GTTTCTGCGC TGTTTCCTGT TGGAGTCCAA CTCGTCCTCA 
GTGCGCTGGC AGGCCCACTG CCTGACACTG CACATCTACA GAAACTCCAA CAAGGCTCAG 
CAGGAGCTCT TGCTAGATCT CATGTGGTCC ATCTGGCCGG AATTGCCAGC TTACGGCCGG 
AAGGCTGCCC AGTTTGTGGA CCTGCTGGGC TATTTCTCCC TGAAAACTGC ACAGACAGAG 
AAGAAGTTGA AGGAGTATTC CCAGAAGGCC GTGGAGATCC TGAGGACTCA GAACCATATC 
CTCACCAATC ACCCCAACTC CAACATCTAC AACACCTTGT CTGGCTTAGT GGAGTTTGAC 
GGCTATTACC TAGAGAGCGA CCCCTGCCTG GTGTGTAACA ATCCTGAGGT GCCGTTTTGT 
TATATCAAGC TGTCTTCCAT TAAAGTGGAC ACACGGTACA CCACCACCCA GCAGGTTGTA 
AAGCTCATTG GCAGTCACAC CATCAGCAAG GTGACAGTGA AAATTGGGGA CCTGAAGCGA 
ACCAAGATGG TGCGGACGAT CAACCTGTAC TACAACAACC GAACTGTGCA GGCCATCGTG 
GAGCTGAAAA ATAAGCCAGC TCGCTGGCAC AAAGCCAAGA AAGTTCAGCT GACTCCCGGA 
CAGACAGAAG TCAAGATTGA CCTGCCTCTG CCCATCGTGG CCTCTAACCT GATGATCGAG 
TTTGCAGACT TCTATGAGAA CTACCAGGCC TCCACAGAGA CCCTTCAATG TCCTCGCTGC 
AGTGCCTCTG TCCCCGCCAA CCCAGGCGTC TGTGGCAACT GTGGAGAGAA TGTATACCAG 
TGTCACAAGT GCAGGTCCAT CAACTATGAT GAAAAGGACC CCTTCCTCTG CAATGCTTGT 
GGCTTCTGTA AATACGCCCG CTTTGACTTC ATGCTTTATG CCAAGCCTTG CTGTGCAGTG 
GATCCCATCG AGAACGAAGA AGATCGGAAG AAGGCCGTTT CCAACATCAA CACACTTCTG 
GACAAAGCTG ACCGCGTGTA CCATCAGCTC ATGGGACATC GGCCCCAGTT GGAGAACCTG 
CTCTGCAAAG TGAATGAAGC AGCTCCAGAA AAGCCACAGG AAGACTCAGG AACAGCGGGA 
GGCATCAGCT CCACTTCAGC CAGTGTGAAT CGCTACATCC TGCAGCTGGC TCAAGAGTAT 
TGTGGAGACT GTAAGAACTC GTTCGATGAG CTCTCCAAAA TCATCCAGAA AGTTTTTGCT 
TCACGCAAAG AGCTGTTGGA ATATGATCTA CAGCAAAGAG AAGCAGCCAC CAAGTCATCC 
CGGACGTCTG TCCAGCCCAC GTTTACTGCC AGCCAGTACC GTGCTTTGTC TGTCTTAGGC 
TGTGGCCACA CCTCCTCCAC CAAGTGCTAT GGCTGTGCCT CAGCTGTCAC GGAGCATTGT 
ATTACACTAC TGAGGGCCCT GGCCACCAAT CCAGCCCTGC GACACATCCT CGTCTCCCAG 
GGCCTCATCC GAGAGCTCTT TGATTACAAC CTCCGCCGTG GCGCTGCAGC CATTCGAGAG 
GAGGTCCGCC AGCTCATGTG CCTCCTCACC AGAGACAACC CAGAGGCCAC CCAGCAAATG 
AACGACCTGA TCATTGGCAA AGTCTCCACT GCACTGAAGG GCCACTGGGC TAATCCTGAC 
CTGGCTAGCA GCCTTCAGTA TGAGATGCTG CTGCTGACAG ATTCCATCTC CAAGGAGGAC 
AGCTGCTGGG AACTCCGCTT ACGATGTGCA CTCAGCCTTT TCCTCATGGC TGTGAACATC 
AAAACGCCAG TGGTTGTTGA GAATATCACA CTCATGTGTC TGCGGATCTT ACAGAAGCTG 
ATTAAACCAC CTGCCCCAAC CAGCAAGAAG AACAAGGATG TCCCCGTGGA GGCCCTCACC 
ACAGTGAAAC CGTACTGCAA TGAGATCCAT GCCCAGGCTC AGCTGTGGCT CAAGAGAGAT 
CCCAAGGCAT CATACGAAGC CTGGAAGAAG TGCCTGCCTA TCCGAGGGGT GGATGGCAAT 
GGGAAATCCC CCAGCAAGTC AGAGCTCCAC CGTCTCTACT TGACCGAGAA GTACGTGTGG 
AGGTGGAAAC AGTTCCTGAG CCGGCGGGGG AAGAGGACCA CCCCACTTGA CCTCAAGCTG 
GGCCACAACA ACTGGCTGCG GCAGGTACTC TTTACCCCAG CAACACAGGC AGCAAGGCAG 
GCAGCCTGTA CCATCGTGGA AGCTCTTGCT ACTGTCCCCA GCCGCAAGCA GCAGGTCCTT 
GACCTCCTCA CCAGTTACCT GGACGAGCTC AGCGTTGCGG GGGAGTGTGC TGCAGAGTAC 
CTGGCTCTCT ACCAGAAGCT CATCGCCTCG TGCCACTGGA AGGTCTACCT AGCTGCACGC 
GGAGTCCTGC CCTACGTGGG CAACCTCATC ACCAAGGAAA TCGCCCGCCT GCTGGCCTTG 
GAGGAGGCCA CGCTGAGCAC AGACCTGCAG CAGGGATATG CCCTCAAGAG TCTCACAGGC 
CTTCTCTCCT CCTTTGTAGA GGTGGAATCC ATTAAGAGGC ATTTTAAGAG CCGCTTAGTG 
GGCACTGTGC TGAATGGGTA CCTGTGCTTG CGGAAGCTGG TGCTGCAGAG GACCAAGCTC 
ATCGACGAGA CCCAGGACAT GCTGCTGGAG ATGCTGGAGG ACATGACCAC AGGTACAGAG 
TCTGAAACCA AGGCCTTCAT GGCTGTGTGC ATTGAGACAG CAAAGCGCTA CAATCTGGAT 
GACTACCGGA CTCCCGTGTT CATCTTTGAG AGGCTGTGCA GCATCATCTA CCCTGAGGAG 
AATGAAGTCA CGGAGTTCTT CGTGACCCTG GAGAAGGACC CCCAGCAAGA GGACTTTCTA 
CAGGGCAGGA TGCCCGGAAA CCCATATAGC AGCAATGAAC CAGGCATTGG GCCTCTTATG 
AGGGACATAA AGAACAAGAT CTGCCAGGAC TGTGACCTAG TGGCTCTTCT GGAGGATGAC 
AGTGGGATGG AGCTCCTGGT GAACAATAAA ATTATCAGTC TGGACCTTCC CGTGGCTGAG 
GTTTACAAGA AGGTGTGGTG TGCGACCAAT GAGGGAGAGC CCATGAGGAT TGTTTATCGA 
ATGCGGGGGC TCCTGGGCGA TGCCACCGAG GAGTTTATCG AGTCCCTGGA CTCCACCACA 
GATGAAGAAG AAGATGAAGA GGAAGTGTAC AGAATGGCCG GTGTGATGGC CCAGTGTGGG 
GGTCTGCAGT GCATGTTGAA CAGACTGGCA GGAGTCAAAG ATTTTAAGCA GGGACGCCAC 
CTTCTAACAG TGCTGCTGAA GCTCTTCAGT TACTGTGTGA AGGTCAAAGT CAACCGGCAG 
CAGCTGGTCA AGCTGGAGAC GAACACTCTG AATGTCATGC TGGGGACTCT GAACCTGGCT 
CTGGTAGCCG AGCAAGAGAG CAAGGACAGC GGAGGAGCAG CTGTGGCTGA GCAGGTGCTA 
AGCATCATGG AGATCATTCT AGATGAATCC AACGCAGAGC CCCTGAGTGA GGACAAGGGC 
AACCTCCTCC TCACAGGTGA CAAGGATCAA CTGGTGATGC TCTTGGACCA GATCAACAGC 
ACCTTCGTTC GATCCAACCC TAGCGTGCTA CAGGGCTTGC TGCGTATCAT CCCATACCTG 
TCCTTCGGAG AGGTGGAGAA GATGCAGATC CTGGTGGAGC GGTTCAAGCC ATACTGCAGC 
TTCGAGAAGT ACGATGAAGA CCATAGTGGG GATGACAAAG TCTTCCTGGA TTGCTTCTGC 
AAAATTGCTG CCGGTATCAA GAACAACAGC AATGGTCATC AGCTGAAGGA TCTCATCCTG 
CAGAAGGGAA TCACCCAAAA TGCCCTGGAC TACATGAAAA AGCATATCCC CAGTGCCAAG 
AATTTGGATG CTGACATCTG GAAAAAGTTT TTGTCTCGCC CTGCTCTGCC ATTTATTTTG 
AGGCTTCTTC GGGGTCTGGC CATGCAGCAC CCTGCCACCC AGGTTCTGAT TGGAACGGAC 
TCCATCACGA GCCTGCATAA GCTGGAGCAG GTGTCCAGTG ATGAGGGCAT TGGGACCCTG 
GCTGAGAACC TGCTGGAGGC CCTGAGAGAA CACCCAGATG TGAACAAGAA GATTGACGCA 
GCCCGCAGGG AGACCCGGGC AGAGAAGAAG CGCATGGCCA TGGCCATGAG GCAGAAGGCT 
CTGGGAACTC TGGGCATGAC GACAAACGAA AAGGGTCAGG TGGTGACCAA GACTGCACTC 
CTGAAGCAGA TGGAAGAGCT GATTGAGGAG CCGGGACTCA CGTGCTGCAT CTGCAGGGAG 
GGGTACAAGT TCCAGCCCAC GAAGGTCTTA GGCATTTACA CTTTCACCAA GCGGGTAGCC 
TTGGAGGAGA TGGAGAACAA GCCCCGGAAG CAGCAGGGCT ACAGCACTGT GTCCCATTTC 
AACATTGTGC ACTACGACTG TCACCTGGCT GCCGTCAGGT TGGCTAGAGG CCGGGAAGAG 
TGGGAGAGTG CTGCCCTGCA GAACGCCAAC ACGAAGTGCA ATGGGCTCCT TCCGGTCTGG 
GGCCCCCATG TCCCTGAATC AGCTTTCGCC ACTTGCTTAG CAAGGCACAA CACGTACCTC 
CAGGAGTGTA CCGGCCAGCG GGAGCCCACG TACCAGCTCA ACATCCATGA CATCAAACTG 
CTCTTCCTGC GCTTCGCCAT GGAGCAGTCA TTCAGTGCAG ACACCGGTGG GGGCGGCCGT 
GAGAGCAACA TCCACCTGAT CCCATACATC ATTCACACTG TGCTTTACGT CCTGAACACG 
ACCCGAGCAA CGTCCCGGGA GGAGAAGAAC CTCCAAGGCT TCCTGGAACA GCCCAAAGAG 
AAGTGGACAG AGAGTGCCTT CGATGTAGAT GGGCCCCACT ACTTCACCAT CCTAGCCCTG 
CACGTCCTCC CCCCTGAACA GTGGAAAGCC ATTCGAGTAG AGATCCTACG CAGGCTGCTG 
GTGGCCTCAC ATGCCCGGGC AGTGGCTCCA GGAGGAGCCA CCAGGTTGAC AGACAAGGCG 
GTGAAGGACT ATTCTGCGTA CCGTTCTTCC CTGCTCTTCT GGGCTCTCGT CGATCTCATT 
TATAACATGT TTAAGAAGGT GCCCACTAGT AACACTGAGG GCGGCTGGTC CTGCTCTCTG 
GCCGAGTACA TCCGCCACAA TGACATGCCC ATCTACGAAG CTGCCGACAA AGCCCTGAAA 
ACCTTCCAGG AGGAGTTCAT GCCAGTGGAG ACCTTCTCGG AGTTCCTCGA CGCAGCAGGT 
CTTCTATCAG AAATCACCGA TCCAGAGAGC TTCCTAAAGG ACCTGTTGAA CTCAGTCCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The N-recognin UBR4 of the N-end rule pathway is targeted to and required for the biogenesis of the early endosome.
Journal of cell science131(17)(2018 09)
Kim ST,Lee YJ,Tasaki T,Mun SR,Hwang J,Kang MJ,Ganipisetti S,Yi EC,Kim BY,Kwon YT

The N-recognin UBR4 of the N-end rule pathway is required for neurogenesis and homeostasis of cell surface proteins.
PloS one13(8)e0202260(2018)
Kim ST,Lee YJ,Tasaki T,Hwang J,Kang MJ,Yi EC,Kim BY,Kwon YT

The ubiquitin ligase Ubr4 controls stability of podocin/MEC-2 supercomplexes.
Human molecular genetics25(7)1328-44(2016 Apr)
Rinschen MM,Bharill P,Wu X,Kohli P,Reinert MJ,Kretz O,Saez I,Schermer B,Höhne M,Bartram MP,Aravamudhan S,Brooks BR,Vilchez D,Huber TB,Müller RU,Krüger M,Benzing T

p600 regulates spindle orientation in apical neural progenitors and contributes to neurogenesis in the developing neocortex.
Biology open3(6)475-85(2014 May)
Belzil C,Asada N,Ishiguro K,Nakaya T,Parsons K,Pendolino V,Neumayer G,Mapelli M,Nakatani Y,Sanada K,Nguyen MD

Time-of-day- and light-dependent expression of ubiquitin protein ligase E3 component N-recognin 4 (UBR4) in the suprachiasmatic nucleus circadian clock.
PloS one9(8)e103103(2014)
Ling HH,Beaulé C,Chiang CK,Tian R,Figeys D,Cheng HY

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Ubr4 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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