Washc3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Washc3
Entrez Gene ID	67282
Full Name	WASH complex subunit 3
Synonyms	2900091E11Rik,5730495F03Rik,Ccdc53
General protein information	Preferred Names WASH complex subunit 3 Names WASH complex subunit 3 WASH complex subunit CCDC53 coiled-coil domain containing 53 coiled-coil domain-containing protein 53
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_027487.1	NP_081763.1	WASH complex subunit 3 isoform 3
XM_006513980.2	XP_006514043.1	WASH complex subunit CCDC53 isoform X1
XM_030245232.1	XP_030101092.1	WASH complex subunit 3 isoform X1
NM_001122960.1	NP_001116432.1	WASH complex subunit 3 isoform 2
NM_026070.3	NP_080346.1	WASH complex subunit 3 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CCDC53	NP_057137.1
Pan troglodytes (chimpanzee)	CCDC53	XP_001156073.1
Bos taurus (cattle)	CCDC53	NP_001073782.1
Gallus gallus (chicken)	CCDC53	XP_004937889.1
Drosophila melanogaster (fruit fly)	CG7429	NP_609178.1
Rattus norvegicus (Norway rat)	Ccdc53	NP_001100246.1
Danio rerio (zebrafish)	ccdc53	NP_956467.1
Xenopus tropicalis (tropical clawed frog)	ccdc53	NP_001016824.1
Canis lupus familiaris (dog)	CCDC53	XP_532668.1
Mus musculus (house mouse)	Ccdc53	NP_080346.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005049	XP_313921.3

Gene Ontology
Bibliography

Related articles in PubMed

Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour.
Tyrrell BJ, Woodham EF, Spence HJ, Strathdee D, Insall RH, Machesky LM
Pigment cell & melanoma research29(5)559-71(2016 09)

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y, Ko NT, Lim MK, Ko MS
Genome research11(9)1553-8(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Washc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Washc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01513	NM_026070.3 Latest version!	Mus musculus WASH complex subunit 3 (Washc3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu04280	NM_027487.1 Latest version!	Mus musculus WASH complex subunit 3 (Washc3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu05789	NM_001122960.1 Latest version!	Mus musculus WASH complex subunit 3 (Washc3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu44715	XM_006513980.2 Latest version!	Mus musculus coiled-coil domain containing 53 (Ccdc53), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu85500	XM_030245232.1 Latest version!	Mus musculus WASH complex subunit 3 (Washc3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Washc3 ( NM_026070.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080346.1 Mus musculus WASH complex subunit 3 (Washc3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu01513
Clone ID Related Accession (Same CDS sequence)	NM_026070.3
Accession Version	NM_026070.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576425600000
Organism	Mus musculus(house mouse)
Product	WASH complex subunit 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK158822.1 and AK013835.1. On Apr 2, 2008 this sequence version replaced NM_026070.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK013835.1, AK158822.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGATGAGG ATGGGCTTCC TCTCATGGGG TCAGGCATAG ACCTGACCAA GGTGCCAGCT 
ATTCAACAGA AAAGAACCGT GGCCTTTCTA AACCAGTTTG TGGTGCACAC TGTGCAGTTT 
CTCAACCGGT TTTCCGCAGT TTGTGAGGAG AAACTGGCAG ACCTTTCTCT CCGGATTCAG 
CAAATTGAAA CAACTCTCAA TATTTTAGAT GCAAAGCTGT CATCTATCCC AGGCCTAGAG 
GACGTCACAG TTGAAGTGTC TCCTTTAAAT GTCACTGCTG TCACAAACGG ATCACATTCT 
GAAACCACTT CGGAGCAAAC GCAGCAGAAT AGCACGCAAG ACTCTGGAGC ACAGGAGAGT 
GAAGCGCCAT CAGAAAATGT CCTGACCGTA GCCAAGGATC CACGGTATGC CAGATACCTC 
AAGATGGTTC AAGTGGGTGT TCCAGTGATG GCAATCAGAG ACAAGATGAT CTCAGAAGGA 
CTGGACCCGG AGCTACTGGA GAAGCCAGAT GCCCCGGTGC CAAATGGCGA AAGCGAAAGA 
GCCGTAGAAG AAAGTTCAGA TAGTGACTCT TCCTTCAGTG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080346.1
CDS	146..730
Translation	MDEDGLPLMGSGIDLTKVPAIQQKRTVAFLNQFVVHTVQFLNRFSAVCEEKLADLSLRIQQIETTLNILDAKLSSIPGLEDVTVEVSPLNVTAVTNGSHSETTSEQTQQNSTQDSGAQESEAPSENVLTVAKDPRYARYLKMVQVGVPVMAIRDKMISEGLDPELLEKPDAPVPNGESERAVEESSDSDSSFSD

Target ORF information:

RefSeq Version	NM_026070.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus WASH complex subunit 3 (Washc3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026070.3

ORF Insert Sequence:

ATGGATGAGG ATGGGCTTCC TCTCATGGGG TCAGGCATAG ACCTGACCAA GGTGCCAGCT 
ATTCAACAGA AAAGAACCGT GGCCTTTCTA AACCAGTTTG TGGTGCACAC TGTGCAGTTT 
CTCAACCGGT TTTCCGCAGT TTGTGAGGAG AAACTGGCAG ACCTTTCTCT CCGGATTCAG 
CAAATTGAAA CAACTCTCAA TATTTTAGAT GCAAAGCTGT CATCTATCCC AGGCCTAGAG 
GACGTCACAG TTGAAGTGTC TCCTTTAAAT GTCACTGCTG TCACAAACGG ATCACATTCT 
GAAACCACTT CGGAGCAAAC GCAGCAGAAT AGCACGCAAG ACTCTGGAGC ACAGGAGAGT 
GAAGCGCCAT CAGAAAATGT CCTGACCGTA GCCAAGGATC CACGGTATGC CAGATACCTC 
AAGATGGTTC AAGTGGGTGT TCCAGTGATG GCAATCAGAG ACAAGATGAT CTCAGAAGGA 
CTGGACCCGG AGCTACTGGA GAAGCCAGAT GCCCCGGTGC CAAATGGCGA AAGCGAAAGA 
GCCGTAGAAG AAAGTTCAGA TAGTGACTCT TCCTTCAGTG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Washc3 ( NM_027487.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081763.1 Mus musculus WASH complex subunit 3 (Washc3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu04280
Clone ID Related Accession (Same CDS sequence)	NM_027487.1
Accession Version	NM_027487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576425600000
Organism	Mus musculus(house mouse)
Product	WASH complex subunit 3 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK158822.1, AK017732.1 and CR514683.1. Transcript Variant: This variant (3) contains an alternate exon that creates a unique 3' coding region and 3' UTR, compared to variant 1, resulting in a shorter protein (isoform 3) with a novel C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK017732.1, AA590676.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGATGAGG ATGGGCTTCC TCTCATGGGG TCAGGCATAG ACCTGACCAA GGTGCCAGCT 
ATTCAACAGA AAAGAACCGT GGCCTTTCTA AACCAGTTTG TGGTGCACAC TGTGCAGTTT 
CTCAACCGGT TTTCCGCAGT TTGTGAGGAG AAACTGGCAG ACCTTTCTCT CCGGATTCAG 
CAAATTGAAA CAACTCTCAA TATTTTAGAT GCAAAGCTGT CATCTATCCC AGGCCTAGAG 
GACGTCACAG TTGAAGTGTC TCCTTTAAAT GTCACTGCTG TCACAAACGG ATCACATTCT 
GAAACCACTT CGGAGCAAAC GCAGCAGAAT AGCACGCAAG ACTCTGGAGC ACAGGAGAGT 
GAAGCGCCAT CAGAAAATGT CCTGACCGTA GCCAAGGATC CACGGTATGC CAGATACCTC 
AAGATGGTTC AAGTGTTCCT GTCCACAGAA CAGGAAGCAG TACCTTGCAT CTTGGCTTCG 
ACGCCCTTTA CGGTTACACA TCTTGGGATG TGGAAACACT GCAGAAAGCA GTTGTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081763.1
CDS	146..685
Translation	MDEDGLPLMGSGIDLTKVPAIQQKRTVAFLNQFVVHTVQFLNRFSAVCEEKLADLSLRIQQIETTLNILDAKLSSIPGLEDVTVEVSPLNVTAVTNGSHSETTSEQTQQNSTQDSGAQESEAPSENVLTVAKDPRYARYLKMVQVFLSTEQEAVPCILASTPFTVTHLGMWKHCRKQLF

Target ORF information:

RefSeq Version	NM_027487.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus WASH complex subunit 3 (Washc3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027487.1

ORF Insert Sequence:

ATGGATGAGG ATGGGCTTCC TCTCATGGGG TCAGGCATAG ACCTGACCAA GGTGCCAGCT 
ATTCAACAGA AAAGAACCGT GGCCTTTCTA AACCAGTTTG TGGTGCACAC TGTGCAGTTT 
CTCAACCGGT TTTCCGCAGT TTGTGAGGAG AAACTGGCAG ACCTTTCTCT CCGGATTCAG 
CAAATTGAAA CAACTCTCAA TATTTTAGAT GCAAAGCTGT CATCTATCCC AGGCCTAGAG 
GACGTCACAG TTGAAGTGTC TCCTTTAAAT GTCACTGCTG TCACAAACGG ATCACATTCT 
GAAACCACTT CGGAGCAAAC GCAGCAGAAT AGCACGCAAG ACTCTGGAGC ACAGGAGAGT 
GAAGCGCCAT CAGAAAATGT CCTGACCGTA GCCAAGGATC CACGGTATGC CAGATACCTC 
AAGATGGTTC AAGTGTTCCT GTCCACAGAA CAGGAAGCAG TACCTTGCAT CTTGGCTTCG 
ACGCCCTTTA CGGTTACACA TCTTGGGATG TGGAAACACT GCAGAAAGCA GTTGTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Washc3 ( NM_001122960.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001116432.1 Mus musculus WASH complex subunit 3 (Washc3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05789
Clone ID Related Accession (Same CDS sequence)	NM_001122960.1
Accession Version	NM_001122960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576425600000
Organism	Mus musculus(house mouse)
Product	WASH complex subunit 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK158822.1, CK795584.1 and AK013835.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: CK795554.1, CK795584.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGATGAGG ATGGGCTTCC TCTCATGGGG TCAGGCATAG ACCTGACCAA GGTGCCAGCT 
ATTCAACAGA AAAGAACCGT GGCCTTTCTA AACCAGTTTG TGGTGCACAC TGTGCAGTTT 
CTCAACCGGT TTTCCGCAGT TTGTGAGGAG AAACTGGCAG ACCTTTCTCT CCGGATTCAG 
CAAATTGAAA CAACTCTCAA TATTTTAGAT GCAAAGCTGT CATCTATCCC AGGCCTAGAG 
GACGTCACAG TTGAAGTGTC TCCTTTAAAT GTCACTGCTG TCACAAACGG ATCACATTCT 
GAAACCACTT CGGAGCAAAC GCAGAATAGC ACGCAAGACT CTGGAGCACA GGAGAGTGAA 
GCGCCATCAG AAAATGTCCT GACCGTAGCC AAGGATCCAC GGTATGCCAG ATACCTCAAG 
ATGGTTCAAG TGGGTGTTCC AGTGATGGCA ATCAGAGACA AGATGATCTC AGAAGGACTG 
GACCCGGAGC TACTGGAGAA GCCAGATGCC CCGGTGCCAA ATGGCGAAAG CGAAAGAGCC 
GTAGAAGAAA GTTCAGATAG TGACTCTTCC TTCAGTGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116432.1
CDS	146..727
Translation	MDEDGLPLMGSGIDLTKVPAIQQKRTVAFLNQFVVHTVQFLNRFSAVCEEKLADLSLRIQQIETTLNILDAKLSSIPGLEDVTVEVSPLNVTAVTNGSHSETTSEQTQNSTQDSGAQESEAPSENVLTVAKDPRYARYLKMVQVGVPVMAIRDKMISEGLDPELLEKPDAPVPNGESERAVEESSDSDSSFSD

Target ORF information:

RefSeq Version	NM_001122960.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus WASH complex subunit 3 (Washc3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122960.1

ORF Insert Sequence:

ATGGATGAGG ATGGGCTTCC TCTCATGGGG TCAGGCATAG ACCTGACCAA GGTGCCAGCT 
ATTCAACAGA AAAGAACCGT GGCCTTTCTA AACCAGTTTG TGGTGCACAC TGTGCAGTTT 
CTCAACCGGT TTTCCGCAGT TTGTGAGGAG AAACTGGCAG ACCTTTCTCT CCGGATTCAG 
CAAATTGAAA CAACTCTCAA TATTTTAGAT GCAAAGCTGT CATCTATCCC AGGCCTAGAG 
GACGTCACAG TTGAAGTGTC TCCTTTAAAT GTCACTGCTG TCACAAACGG ATCACATTCT 
GAAACCACTT CGGAGCAAAC GCAGAATAGC ACGCAAGACT CTGGAGCACA GGAGAGTGAA 
GCGCCATCAG AAAATGTCCT GACCGTAGCC AAGGATCCAC GGTATGCCAG ATACCTCAAG 
ATGGTTCAAG TGGGTGTTCC AGTGATGGCA ATCAGAGACA AGATGATCTC AGAAGGACTG 
GACCCGGAGC TACTGGAGAA GCCAGATGCC CCGGTGCCAA ATGGCGAAAG CGAAAGAGCC 
GTAGAAGAAA GTTCAGATAG TGACTCTTCC TTCAGTGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Washc3 ( XM_006513980.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514043.1 Mus musculus coiled-coil domain containing 53 (Ccdc53), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu44715
Clone ID Related Accession (Same CDS sequence)	XM_006513980.2
Accession Version	XM_006513980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	WASH complex subunit CCDC53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG ATGGGCTTCC TCTCATGGGG TCAGGCATAG ACCTGACCAA GGTGCCAGCT 
ATTCAACAGA AAAGAACCGT GGCCTTTCTA AACCAGTTTG TGGTGCACAC TGTGCAGTTT 
CTCAACCGGT TTTCCGCAGT TTGTGAGGAG AAACTGGCAG ACCTTTCTCT CCGGATTCAG 
CAAATTGAAA CAACTCTCAA TATTTTAGAT GCAAAGCTGT CATCTATCCC AGGCCTAGAG 
GACGTCACAG TTGAAGTGTC TCCTTTAAAT GTCACTGCTG TCACAAACGG ATCACATTCT 
GAAACCACTT CGGAGCAAAC GCAGCAGAAT AGCACGCAAG ACTCTGGAGC ACAGGAGAGT 
GAAGCGCCAT CAGAAAATGT CCTGACCGTA GCCAAGGATC CACGGTATGC CAGATACCTC 
AAGATGGTTC AAGTGGGTGT TCCAGTGATG GCAATCAGAG ACAAGATGAT CTCAGAAGGA 
CTGGACCCGG AGCTACTGGA GGACATCGTG AGAAGGAACA GCTTGTTCGC ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514043.1
CDS	138..674
Translation	MDEDGLPLMGSGIDLTKVPAIQQKRTVAFLNQFVVHTVQFLNRFSAVCEEKLADLSLRIQQIETTLNILDAKLSSIPGLEDVTVEVSPLNVTAVTNGSHSETTSEQTQQNSTQDSGAQESEAPSENVLTVAKDPRYARYLKMVQVGVPVMAIRDKMISEGLDPELLEDIVRRNSLFAQ

Target ORF information:

RefSeq Version	XM_006513980.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus coiled-coil domain containing 53 (Ccdc53), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513980.2

ORF Insert Sequence:

ATGGATGAGG ATGGGCTTCC TCTCATGGGG TCAGGCATAG ACCTGACCAA GGTGCCAGCT 
ATTCAACAGA AAAGAACCGT GGCCTTTCTA AACCAGTTTG TGGTGCACAC TGTGCAGTTT 
CTCAACCGGT TTTCCGCAGT TTGTGAGGAG AAACTGGCAG ACCTTTCTCT CCGGATTCAG 
CAAATTGAAA CAACTCTCAA TATTTTAGAT GCAAAGCTGT CATCTATCCC AGGCCTAGAG 
GACGTCACAG TTGAAGTGTC TCCTTTAAAT GTCACTGCTG TCACAAACGG ATCACATTCT 
GAAACCACTT CGGAGCAAAC GCAGCAGAAT AGCACGCAAG ACTCTGGAGC ACAGGAGAGT 
GAAGCGCCAT CAGAAAATGT CCTGACCGTA GCCAAGGATC CACGGTATGC CAGATACCTC 
AAGATGGTTC AAGTGGGTGT TCCAGTGATG GCAATCAGAG ACAAGATGAT CTCAGAAGGA 
CTGGACCCGG AGCTACTGGA GGACATCGTG AGAAGGAACA GCTTGTTCGC ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Washc3 ( XM_030245232.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101092.1 Mus musculus WASH complex subunit 3 (Washc3), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu85500
Clone ID Related Accession (Same CDS sequence)	XM_030245232.1
Accession Version	XM_030245232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	WASH complex subunit 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG ATGGGCTTCC TCTCATGGGG TCAGGCATAG ACCTGACCAA GGTGCCAGCT 
ATTCAACAGA AAAGAACCGT GGCCTTTCTA AACCAGTTTG TGGTGCACAC TGTGCAGTTT 
CTCAACCGGT TTTCCGCAGT TTGTGAGGAG AAACTGGCAG ACCTTTCTCT CCGGATTCAG 
CAAATTGAAA CAACTCTCAA TATTTTAGAT GCAAAGCTGT CATCTATCCC AGGCCTAGAG 
GACGTCACAG TTGAAGTGTC TCCTTTAAAT GTCACTGCTG TCACAAACGG ATCACATTCT 
GAAACCACTT CGGAGCAAAC GCAGAATAGC ACGCAAGACT CTGGAGCACA GGAGAGTGAA 
GCGCCATCAG AAAATGTCCT GACCGTAGCC AAGGATCCAC GGTATGCCAG ATACCTCAAG 
ATGGTTCAAG TGTTCCTGTC CACAGAACAG GAAGCAGTAC CTTGCATCTT GGCTTCGACG 
CCCTTTACGG TTACACATCT TGGGATGTGG AAACACTGCA GAAAGCAGTT GTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101092.1
CDS	143..679
Translation	MDEDGLPLMGSGIDLTKVPAIQQKRTVAFLNQFVVHTVQFLNRFSAVCEEKLADLSLRIQQIETTLNILDAKLSSIPGLEDVTVEVSPLNVTAVTNGSHSETTSEQTQNSTQDSGAQESEAPSENVLTVAKDPRYARYLKMVQVFLSTEQEAVPCILASTPFTVTHLGMWKHCRKQLF

Target ORF information:

RefSeq Version	XM_030245232.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus WASH complex subunit 3 (Washc3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245232.1

ORF Insert Sequence:

ATGGATGAGG ATGGGCTTCC TCTCATGGGG TCAGGCATAG ACCTGACCAA GGTGCCAGCT 
ATTCAACAGA AAAGAACCGT GGCCTTTCTA AACCAGTTTG TGGTGCACAC TGTGCAGTTT 
CTCAACCGGT TTTCCGCAGT TTGTGAGGAG AAACTGGCAG ACCTTTCTCT CCGGATTCAG 
CAAATTGAAA CAACTCTCAA TATTTTAGAT GCAAAGCTGT CATCTATCCC AGGCCTAGAG 
GACGTCACAG TTGAAGTGTC TCCTTTAAAT GTCACTGCTG TCACAAACGG ATCACATTCT 
GAAACCACTT CGGAGCAAAC GCAGAATAGC ACGCAAGACT CTGGAGCACA GGAGAGTGAA 
GCGCCATCAG AAAATGTCCT GACCGTAGCC AAGGATCCAC GGTATGCCAG ATACCTCAAG 
ATGGTTCAAG TGTTCCTGTC CACAGAACAG GAAGCAGTAC CTTGCATCTT GGCTTCGACG 
CCCTTTACGG TTACACATCT TGGGATGTGG AAACACTGCA GAAAGCAGTT GTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour.
Pigment cell & melanoma research29(5)559-71(2016 09)
Tyrrell BJ,Woodham EF,Spence HJ,Strathdee D,Insall RH,Machesky LM

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Genome research11(9)1553-8(2001 Sep)
Piao Y,Ko NT,Lim MK,Ko MS

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Washc3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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