Nepn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepn
Entrez Gene ID	66650
Full Name	nephrocan
Synonyms	5730521E12Rik,Npn,periolin
General protein information	Preferred Names nephrocan Names nephrocan
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006512818.4	XP_006512881.1	nephrocan isoform X1
XM_006512818.3	XP_006512881.1	nephrocan isoform X1
XM_006512819.3	XP_006512882.1	nephrocan isoform X2
XM_006512819.4	XP_006512882.1	nephrocan isoform X2
NM_025684.2	NP_079960.1	nephrocan precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NEPN	NP_001096797.1
Mus musculus (house mouse)	Nepn	NP_079960.1
Rattus norvegicus (Norway rat)	Nepn	NP_001101102.1
Canis lupus familiaris (dog)	NEPN	XP_541218.2
Gallus gallus (chicken)	LOC428618	XP_426175.3

Gene Ontology
Bibliography

Related articles in PubMed

A regulatory network controls nephrocan expression and midgut patterning.
Hou J, Wei W, Saund RS, Xiang P, Cunningham TJ, Yi Y, Alder O, Lu DY, Savory JG, Krentz NA, Montpetit R, Cullum R, Hofs N, Lohnes D, Humphries RK, Yamanaka Y, Duester G, Saijoh Y, Hoodless PA
Development (Cambridge, England)141(19)3772-81(2014 Oct)

Loss of function of mouse Pax-Interacting Protein 1-associated glutamate rich protein 1a (Pagr1a) leads to reduced Bmp2 expression and defects in chorion and amnion development.
Kumar A, Lualdi M, Loncarek J, Cho YW, Lee JE, Ge K, Kuehn MR
Developmental dynamics : an official publication of the American Association of Anatomists243(7)937-47(2014 Jul)

miR-335 promotes mesendodermal lineage segregation and shapes a transcription factor gradient in the endoderm.
Yang D, Lutter D, Burtscher I, Uetzmann L, Theis FJ, Lickert H
Development (Cambridge, England)141(3)514-25(2014 Feb)

Wnt/β-catenin signalling regulates Sox17 expression and is essential for organizer and endoderm formation in the mouse.
Engert S, Burtscher I, Liao WP, Dulev S, Schotta G, Lickert H
Development (Cambridge, England)140(15)3128-38(2013 Aug)

Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord.
Tamplin OJ, Cox BJ, Rossant J
Developmental biology360(2)415-25(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu43929	XM_006512819.4 Latest version!	Mus musculus nephrocan (Nepn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu00872	NM_025684.2 Latest version!	Mus musculus nephrocan (Nepn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu43928	XM_006512818.4 Latest version!	Mus musculus nephrocan (Nepn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu43928	XM_006512818.3	Mus musculus nephrocan (Nepn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu43929	XM_006512819.3	Mus musculus nephrocan (Nepn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepn ( XM_006512819.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512882.1 Mus musculus nephrocan (Nepn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu43929
Clone ID Related Accession (Same CDS sequence)	XM_006512819.3 , XM_006512819.4
Accession Version	XM_006512819.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	nephrocan isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512819.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCTAT CTAACTTCAC TGGACTGTTG GCTCTGGAAG ATTTTATTCT GCTGGCCAGT 
GGAACAGAGT CTATTGAAAA TGACACCTTC AAAACTCTGA GTACACTAAA GACTCTAGAA 
CTTTGGAAAA ATAAATTGAG ACAAGTCCCC AGTGCTCTCC CAGCCAACCT TGAGGTGTTA 
AAATTAAATG ATAATGCCAT ATGTGCTCTA CGTGGATCAG AGTTTGAAGG ACTGAAGAAC 
TTGAAAGTCC TTGAACTTAA AAACAACTTG ATCTCGTCCC TGTCTCCCAG CATGCTCTCC 
CCACTTGCCA GTTTGCAGAG TTTGATGGTG GATGGTAACA ACATAGAGTC CGTGGTTGGA 
CCTCTGAGCC TTCCGCATCT CAAGTACATG AGCATGGAGA ACAACCAACT CCACCTTATA 
CCAGGGAATG TCTTCACTTC CCTACAGAAT TTGCAGTTCC TCAGTTTCAG TGGTAATTTT 
CTAACTAAAA TTCCCATAAA TCTACCCAAA TCCTTGCTAT CTTTAAAGAT GGAGAGAAAC 
CAGCTCAAAG TGGTCAGGTT TCGCGATATG AAACACTTGG AGAACCTGTC CCATCTTTAC 
CTGTCAGAGA ACTTCCTGTC TTCCATTGAT GGGGCACAGC AGCTGACCAA TTTAACAACT 
CTGGAGGTTT CCCAAAACCA GCTTCAAATG TTGCCTCCCA GGCTACCATC GAGGTTACAG 
AAGCTTGATT GTAGCAGTAA CTTCATTCAG AGAGTGACAG CACCAGAATT CCAGGACCTC 
CGAGACCTGA AACATCTGTT TCTAGACAAC AATGTTGTCA GCTTGTTCGA GGCTGGGGCC 
CTTCAGAGGT GTTCTCAGCT GTCCAACCTG GCCCTGGAGC AGAATCTGCT GTTGTCTATC 
CCTCTGAGGC TCCCTAAGAC CCTGGCCAGG CTGGACCTCA AGGGCAATGC CATCCAGGAC 
ATGGCTGAAA GGGAGCTCAG GGACCTCAAA CAACTGCAGG TCCTCAACCT CAGGAACAAC 
CGGATCTCGG CCTTAGATTT CAAGGCACTG GAGGGGCTGC CTCGGCTCAG GCATCTCTAC 
CTGGATGGGA ATCCCTGGAA CTGCACTTGC AGTCTCCTAC GAGCCAGGGA AGTCCTGAAG 
GCCAAGGGCA CGGACGTGAA AGGGGGACAG TGCGCAGCCC CAGCAGAGCG ACAGGGGGAG 
AGCTGGATGT CCTCCAAGAA GATCCTGAGG CAATGTGAGC ATCACTTACA GCAGAGTGAG 
AAAAGCAAAG AGACCAAGAA GAAACCCAAA CCCGAAGACT CTTCCAGCAT CAGACTCAAC 
ATGGATGACG ACGATGACGA TTATGAAATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512882.1
CDS	256..1611
Translation	MQLSNFTGLLALEDFILLASGTESIENDTFKTLSTLKTLELWKNKLRQVPSALPANLEVLKLNDNAICALRGSEFEGLKNLKVLELKNNLISSLSPSMLSPLASLQSLMVDGNNIESVVGPLSLPHLKYMSMENNQLHLIPGNVFTSLQNLQFLSFSGNFLTKIPINLPKSLLSLKMERNQLKVVRFRDMKHLENLSHLYLSENFLSSIDGAQQLTNLTTLEVSQNQLQMLPPRLPSRLQKLDCSSNFIQRVTAPEFQDLRDLKHLFLDNNVVSLFEAGALQRCSQLSNLALEQNLLLSIPLRLPKTLARLDLKGNAIQDMAERELRDLKQLQVLNLRNNRISALDFKALEGLPRLRHLYLDGNPWNCTCSLLRAREVLKAKGTDVKGGQCAAPAERQGESWMSSKKILRQCEHHLQQSEKSKETKKKPKPEDSSSIRLNMDDDDDDYEID

Target ORF information:

RefSeq Version	XM_006512819.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nephrocan (Nepn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512819.3

ORF Insert Sequence:

ATGCAGCTAT CTAACTTCAC TGGACTGTTG GCTCTGGAAG ATTTTATTCT GCTGGCCAGT 
GGAACAGAGT CTATTGAAAA TGACACCTTC AAAACTCTGA GTACACTAAA GACTCTAGAA 
CTTTGGAAAA ATAAATTGAG ACAAGTCCCC AGTGCTCTCC CAGCCAACCT TGAGGTGTTA 
AAATTAAATG ATAATGCCAT ATGTGCTCTA CGTGGATCAG AGTTTGAAGG ACTGAAGAAC 
TTGAAAGTCC TTGAACTTAA AAACAACTTG ATCTCGTCCC TGTCTCCCAG CATGCTCTCC 
CCACTTGCCA GTTTGCAGAG TTTGATGGTG GATGGTAACA ACATAGAGTC CGTGGTTGGA 
CCTCTGAGCC TTCCGCATCT CAAGTACATG AGCATGGAGA ACAACCAACT CCACCTTATA 
CCAGGGAATG TCTTCACTTC CCTACAGAAT TTGCAGTTCC TCAGTTTCAG TGGTAATTTT 
CTAACTAAAA TTCCCATAAA TCTACCCAAA TCCTTGCTAT CTTTAAAGAT GGAGAGAAAC 
CAGCTCAAAG TGGTCAGGTT TCGCGATATG AAACACTTGG AGAACCTGTC CCATCTTTAC 
CTGTCAGAGA ACTTCCTGTC TTCCATTGAT GGGGCACAGC AGCTGACCAA TTTAACAACT 
CTGGAGGTTT CCCAAAACCA GCTTCAAATG TTGCCTCCCA GGCTACCATC GAGGTTACAG 
AAGCTTGATT GTAGCAGTAA CTTCATTCAG AGAGTGACAG CACCAGAATT CCAGGACCTC 
CGAGACCTGA AACATCTGTT TCTAGACAAC AATGTTGTCA GCTTGTTCGA GGCTGGGGCC 
CTTCAGAGGT GTTCTCAGCT GTCCAACCTG GCCCTGGAGC AGAATCTGCT GTTGTCTATC 
CCTCTGAGGC TCCCTAAGAC CCTGGCCAGG CTGGACCTCA AGGGCAATGC CATCCAGGAC 
ATGGCTGAAA GGGAGCTCAG GGACCTCAAA CAACTGCAGG TCCTCAACCT CAGGAACAAC 
CGGATCTCGG CCTTAGATTT CAAGGCACTG GAGGGGCTGC CTCGGCTCAG GCATCTCTAC 
CTGGATGGGA ATCCCTGGAA CTGCACTTGC AGTCTCCTAC GAGCCAGGGA AGTCCTGAAG 
GCCAAGGGCA CGGACGTGAA AGGGGGACAG TGCGCAGCCC CAGCAGAGCG ACAGGGGGAG 
AGCTGGATGT CCTCCAAGAA GATCCTGAGG CAATGTGAGC ATCACTTACA GCAGAGTGAG 
AAAAGCAAAG AGACCAAGAA GAAACCCAAA CCCGAAGACT CTTCCAGCAT CAGACTCAAC 
ATGGATGACG ACGATGACGA TTATGAAATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nepn ( XM_006512819.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512882.1 Mus musculus nephrocan (Nepn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu43929
Clone ID Related Accession (Same CDS sequence)	XM_006512819.3 , XM_006512819.4
Accession Version	XM_006512819.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	nephrocan isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512819.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCTAT CTAACTTCAC TGGACTGTTG GCTCTGGAAG ATTTTATTCT GCTGGCCAGT 
GGAACAGAGT CTATTGAAAA TGACACCTTC AAAACTCTGA GTACACTAAA GACTCTAGAA 
CTTTGGAAAA ATAAATTGAG ACAAGTCCCC AGTGCTCTCC CAGCCAACCT TGAGGTGTTA 
AAATTAAATG ATAATGCCAT ATGTGCTCTA CGTGGATCAG AGTTTGAAGG ACTGAAGAAC 
TTGAAAGTCC TTGAACTTAA AAACAACTTG ATCTCGTCCC TGTCTCCCAG CATGCTCTCC 
CCACTTGCCA GTTTGCAGAG TTTGATGGTG GATGGTAACA ACATAGAGTC CGTGGTTGGA 
CCTCTGAGCC TTCCGCATCT CAAGTACATG AGCATGGAGA ACAACCAACT CCACCTTATA 
CCAGGGAATG TCTTCACTTC CCTACAGAAT TTGCAGTTCC TCAGTTTCAG TGGTAATTTT 
CTAACTAAAA TTCCCATAAA TCTACCCAAA TCCTTGCTAT CTTTAAAGAT GGAGAGAAAC 
CAGCTCAAAG TGGTCAGGTT TCGCGATATG AAACACTTGG AGAACCTGTC CCATCTTTAC 
CTGTCAGAGA ACTTCCTGTC TTCCATTGAT GGGGCACAGC AGCTGACCAA TTTAACAACT 
CTGGAGGTTT CCCAAAACCA GCTTCAAATG TTGCCTCCCA GGCTACCATC GAGGTTACAG 
AAGCTTGATT GTAGCAGTAA CTTCATTCAG AGAGTGACAG CACCAGAATT CCAGGACCTC 
CGAGACCTGA AACATCTGTT TCTAGACAAC AATGTTGTCA GCTTGTTCGA GGCTGGGGCC 
CTTCAGAGGT GTTCTCAGCT GTCCAACCTG GCCCTGGAGC AGAATCTGCT GTTGTCTATC 
CCTCTGAGGC TCCCTAAGAC CCTGGCCAGG CTGGACCTCA AGGGCAATGC CATCCAGGAC 
ATGGCTGAAA GGGAGCTCAG GGACCTCAAA CAACTGCAGG TCCTCAACCT CAGGAACAAC 
CGGATCTCGG CCTTAGATTT CAAGGCACTG GAGGGGCTGC CTCGGCTCAG GCATCTCTAC 
CTGGATGGGA ATCCCTGGAA CTGCACTTGC AGTCTCCTAC GAGCCAGGGA AGTCCTGAAG 
GCCAAGGGCA CGGACGTGAA AGGGGGACAG TGCGCAGCCC CAGCAGAGCG ACAGGGGGAG 
AGCTGGATGT CCTCCAAGAA GATCCTGAGG CAATGTGAGC ATCACTTACA GCAGAGTGAG 
AAAAGCAAAG AGACCAAGAA GAAACCCAAA CCCGAAGACT CTTCCAGCAT CAGACTCAAC 
ATGGATGACG ACGATGACGA TTATGAAATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512882.1
CDS	256..1611
Translation	MQLSNFTGLLALEDFILLASGTESIENDTFKTLSTLKTLELWKNKLRQVPSALPANLEVLKLNDNAICALRGSEFEGLKNLKVLELKNNLISSLSPSMLSPLASLQSLMVDGNNIESVVGPLSLPHLKYMSMENNQLHLIPGNVFTSLQNLQFLSFSGNFLTKIPINLPKSLLSLKMERNQLKVVRFRDMKHLENLSHLYLSENFLSSIDGAQQLTNLTTLEVSQNQLQMLPPRLPSRLQKLDCSSNFIQRVTAPEFQDLRDLKHLFLDNNVVSLFEAGALQRCSQLSNLALEQNLLLSIPLRLPKTLARLDLKGNAIQDMAERELRDLKQLQVLNLRNNRISALDFKALEGLPRLRHLYLDGNPWNCTCSLLRAREVLKAKGTDVKGGQCAAPAERQGESWMSSKKILRQCEHHLQQSEKSKETKKKPKPEDSSSIRLNMDDDDDDYEID

Target ORF information:

RefSeq Version	XM_006512819.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nephrocan (Nepn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512819.4

ORF Insert Sequence:

ATGCAGCTAT CTAACTTCAC TGGACTGTTG GCTCTGGAAG ATTTTATTCT GCTGGCCAGT 
GGAACAGAGT CTATTGAAAA TGACACCTTC AAAACTCTGA GTACACTAAA GACTCTAGAA 
CTTTGGAAAA ATAAATTGAG ACAAGTCCCC AGTGCTCTCC CAGCCAACCT TGAGGTGTTA 
AAATTAAATG ATAATGCCAT ATGTGCTCTA CGTGGATCAG AGTTTGAAGG ACTGAAGAAC 
TTGAAAGTCC TTGAACTTAA AAACAACTTG ATCTCGTCCC TGTCTCCCAG CATGCTCTCC 
CCACTTGCCA GTTTGCAGAG TTTGATGGTG GATGGTAACA ACATAGAGTC CGTGGTTGGA 
CCTCTGAGCC TTCCGCATCT CAAGTACATG AGCATGGAGA ACAACCAACT CCACCTTATA 
CCAGGGAATG TCTTCACTTC CCTACAGAAT TTGCAGTTCC TCAGTTTCAG TGGTAATTTT 
CTAACTAAAA TTCCCATAAA TCTACCCAAA TCCTTGCTAT CTTTAAAGAT GGAGAGAAAC 
CAGCTCAAAG TGGTCAGGTT TCGCGATATG AAACACTTGG AGAACCTGTC CCATCTTTAC 
CTGTCAGAGA ACTTCCTGTC TTCCATTGAT GGGGCACAGC AGCTGACCAA TTTAACAACT 
CTGGAGGTTT CCCAAAACCA GCTTCAAATG TTGCCTCCCA GGCTACCATC GAGGTTACAG 
AAGCTTGATT GTAGCAGTAA CTTCATTCAG AGAGTGACAG CACCAGAATT CCAGGACCTC 
CGAGACCTGA AACATCTGTT TCTAGACAAC AATGTTGTCA GCTTGTTCGA GGCTGGGGCC 
CTTCAGAGGT GTTCTCAGCT GTCCAACCTG GCCCTGGAGC AGAATCTGCT GTTGTCTATC 
CCTCTGAGGC TCCCTAAGAC CCTGGCCAGG CTGGACCTCA AGGGCAATGC CATCCAGGAC 
ATGGCTGAAA GGGAGCTCAG GGACCTCAAA CAACTGCAGG TCCTCAACCT CAGGAACAAC 
CGGATCTCGG CCTTAGATTT CAAGGCACTG GAGGGGCTGC CTCGGCTCAG GCATCTCTAC 
CTGGATGGGA ATCCCTGGAA CTGCACTTGC AGTCTCCTAC GAGCCAGGGA AGTCCTGAAG 
GCCAAGGGCA CGGACGTGAA AGGGGGACAG TGCGCAGCCC CAGCAGAGCG ACAGGGGGAG 
AGCTGGATGT CCTCCAAGAA GATCCTGAGG CAATGTGAGC ATCACTTACA GCAGAGTGAG 
AAAAGCAAAG AGACCAAGAA GAAACCCAAA CCCGAAGACT CTTCCAGCAT CAGACTCAAC 
ATGGATGACG ACGATGACGA TTATGAAATA GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nepn ( NM_025684.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079960.1 Mus musculus nephrocan (Nepn), mRNA.

Price & Availability↑

CloneID	OMu00872
Clone ID Related Accession (Same CDS sequence)	NM_025684.2
Accession Version	NM_025684.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Mus musculus(house mouse)
Product	nephrocan precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK017780.1. On Apr 6, 2007 this sequence version replaced NM_025684.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK017780.1, AY938536.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849379 [ECO:0000348] ##Evidence-Data-END##

ATGCACCCGC TTTGGGCTTT TCTTTTGGGG CTCTCTCTTA CCAATGGCCT CAGTGCAAAC 
TGTCCAGGAC GATGCAGTTG TGATTCTATG CAGTCTGTAC AATGCTACAG GCTCATGGAA 
CTCCCATCAG GCATTCCTTC CACCACCAAG AGACTCTACA TTAGCCACAG CAGAATCCAG 
CACCTTCAGC TATCTAACTT CACTGGACTG TTGGCTCTGG AAGATTTTAT TCTGCTGGCC 
AGTGGAACAG AGTCTATTGA AAATGACACC TTCAAAACTC TGAGTACACT AAAGACTCTA 
GAACTTTGGA AAAATAAATT GAGACAAGTC CCCAGTGCTC TCCCAGCCAA CCTTGAGGTG 
TTAAAATTAA ATGATAATGC CATATGTGCT CTACGTGGAT CAGAGTTTGA AGGACTGAAG 
AACTTGAAAG TCCTTGAACT TAAAAACAAC TTGATCTCGT CCCTGTCTCC CAGCATGCTC 
TCCCCACTTG CCAGTTTGCA GAGTTTGATG GTGGATGGTA ACAACATAGA GTCCGTGGTT 
GGACCTCTGA GCCTTCCGCA TCTCAAGTAC ATGAGCATGG AGAACAACCA ACTCCACCTT 
ATACCAGGGA ATGTCTTCAC TTCCCTACAG AATTTGCAGT TCCTCAGTTT CAGTGGTAAT 
TTTCTAACTA AAATTCCCAT AAATCTACCC AAATCCTTGC TATCTTTAAA GATGGAGAGA 
AACCAGCTCA AAGTGGTCAG GTTTCGCGAT ATGAAACACT TGGAGAACCT GTCCCATCTT 
TACCTGTCAG AGAACTTCCT GTCTTCCATT GATGGGGCAC AGCAGCTGAC CAATTTAACA 
ACTCTGGAGG TTTCCCAAAA CCAGCTTCAA ATGTTGCCTC CCAGGCTACC ATCGAGGTTA 
CAGAAGCTTG ATTGTAGCAG TAACTTCATT CAGAGAGTGA CAGCACCAGA ATTCCAGGAC 
CTCCGAGACC TGAAACATCT GTTTCTAGAC AACAATGTTG TCAGCTTGTT CGAGGCTGGG 
GCCCTTCAGA GGTGTTCTCA GCTGTCCAAC CTGGCCCTGG AGCAGAATCT GCTGTTGTCT 
ATCCCTCTGA GGCTCCCTAA GACCCTGGCC AGGCTGGACC TCAAGGGCAA TGCCATCCAG 
GACATGGCTG AAAGGGAGCT CAGGGACCTC AAACAACTGC AGGTCCTCAA CCTCAGGAAC 
AACCGGATCT CGGCCTTAGA TTTCAAGGCA CTGGAGGGGC TGCCTCGGCT CAGGCATCTC 
TACCTGGATG GGAATCCCTG GAACTGCACT TGCAGTCTCC TACGAGCCAG GGAAGTCCTG 
AAGGCCAAGG GCACGGACGT GAAAGGGGGA CAGTGCGCAG CCCCAGCAGA GCGACAGGGG 
GAGAGCTGGA TGTCCTCCAA GAAGATCCTG AGGCAATGTG AGCATCACTT ACAGCAGAGT 
GAGAAAAGCA AAGAGACCAA GAAGAAACCC AAACCCGAAG ACTCTTCCAG CATCAGACTC 
AACATGGATG ACGACGATGA CGATTATGAA ATAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079960.1
CDS	33..1571
Translation	MHPLWAFLLGLSLTNGLSANCPGRCSCDSMQSVQCYRLMELPSGIPSTTKRLYISHSRIQHLQLSNFTGLLALEDFILLASGTESIENDTFKTLSTLKTLELWKNKLRQVPSALPANLEVLKLNDNAICALRGSEFEGLKNLKVLELKNNLISSLSPSMLSPLASLQSLMVDGNNIESVVGPLSLPHLKYMSMENNQLHLIPGNVFTSLQNLQFLSFSGNFLTKIPINLPKSLLSLKMERNQLKVVRFRDMKHLENLSHLYLSENFLSSIDGAQQLTNLTTLEVSQNQLQMLPPRLPSRLQKLDCSSNFIQRVTAPEFQDLRDLKHLFLDNNVVSLFEAGALQRCSQLSNLALEQNLLLSIPLRLPKTLARLDLKGNAIQDMAERELRDLKQLQVLNLRNNRISALDFKALEGLPRLRHLYLDGNPWNCTCSLLRAREVLKAKGTDVKGGQCAAPAERQGESWMSSKKILRQCEHHLQQSEKSKETKKKPKPEDSSSIRLNMDDDDDDYEID

Target ORF information:

RefSeq Version	NM_025684.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nephrocan (Nepn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025684.2

ORF Insert Sequence:

ATGCACCCGC TTTGGGCTTT TCTTTTGGGG CTCTCTCTTA CCAATGGCCT CAGTGCAAAC 
TGTCCAGGAC GATGCAGTTG TGATTCTATG CAGTCTGTAC AATGCTACAG GCTCATGGAA 
CTCCCATCAG GCATTCCTTC CACCACCAAG AGACTCTACA TTAGCCACAG CAGAATCCAG 
CACCTTCAGC TATCTAACTT CACTGGACTG TTGGCTCTGG AAGATTTTAT TCTGCTGGCC 
AGTGGAACAG AGTCTATTGA AAATGACACC TTCAAAACTC TGAGTACACT AAAGACTCTA 
GAACTTTGGA AAAATAAATT GAGACAAGTC CCCAGTGCTC TCCCAGCCAA CCTTGAGGTG 
TTAAAATTAA ATGATAATGC CATATGTGCT CTACGTGGAT CAGAGTTTGA AGGACTGAAG 
AACTTGAAAG TCCTTGAACT TAAAAACAAC TTGATCTCGT CCCTGTCTCC CAGCATGCTC 
TCCCCACTTG CCAGTTTGCA GAGTTTGATG GTGGATGGTA ACAACATAGA GTCCGTGGTT 
GGACCTCTGA GCCTTCCGCA TCTCAAGTAC ATGAGCATGG AGAACAACCA ACTCCACCTT 
ATACCAGGGA ATGTCTTCAC TTCCCTACAG AATTTGCAGT TCCTCAGTTT CAGTGGTAAT 
TTTCTAACTA AAATTCCCAT AAATCTACCC AAATCCTTGC TATCTTTAAA GATGGAGAGA 
AACCAGCTCA AAGTGGTCAG GTTTCGCGAT ATGAAACACT TGGAGAACCT GTCCCATCTT 
TACCTGTCAG AGAACTTCCT GTCTTCCATT GATGGGGCAC AGCAGCTGAC CAATTTAACA 
ACTCTGGAGG TTTCCCAAAA CCAGCTTCAA ATGTTGCCTC CCAGGCTACC ATCGAGGTTA 
CAGAAGCTTG ATTGTAGCAG TAACTTCATT CAGAGAGTGA CAGCACCAGA ATTCCAGGAC 
CTCCGAGACC TGAAACATCT GTTTCTAGAC AACAATGTTG TCAGCTTGTT CGAGGCTGGG 
GCCCTTCAGA GGTGTTCTCA GCTGTCCAAC CTGGCCCTGG AGCAGAATCT GCTGTTGTCT 
ATCCCTCTGA GGCTCCCTAA GACCCTGGCC AGGCTGGACC TCAAGGGCAA TGCCATCCAG 
GACATGGCTG AAAGGGAGCT CAGGGACCTC AAACAACTGC AGGTCCTCAA CCTCAGGAAC 
AACCGGATCT CGGCCTTAGA TTTCAAGGCA CTGGAGGGGC TGCCTCGGCT CAGGCATCTC 
TACCTGGATG GGAATCCCTG GAACTGCACT TGCAGTCTCC TACGAGCCAG GGAAGTCCTG 
AAGGCCAAGG GCACGGACGT GAAAGGGGGA CAGTGCGCAG CCCCAGCAGA GCGACAGGGG 
GAGAGCTGGA TGTCCTCCAA GAAGATCCTG AGGCAATGTG AGCATCACTT ACAGCAGAGT 
GAGAAAAGCA AAGAGACCAA GAAGAAACCC AAACCCGAAG ACTCTTCCAG CATCAGACTC 
AACATGGATG ACGACGATGA CGATTATGAA ATAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nepn ( XM_006512818.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512881.1 Mus musculus nephrocan (Nepn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43928
Clone ID Related Accession (Same CDS sequence)	XM_006512818.4 , XM_006512818.3
Accession Version	XM_006512818.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	nephrocan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512818.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAA TGGAGTTACC CCCAACCCCC TGTCTTCTCA CAAGGACAAG CACACATTCA 
AAAGATTCTG TTATCAGACT CCGTGGTATA AAAAGTAAGG TGTCACCTGA ACAGTTGCTT 
GCTACTTGCA AACCTCTGTG TTGGACAATG CACCCGCTTT GGGCTTTTCT TTTGGGGCTC 
TCTCTTACCA ATGGCCTCAG TGCAAACTGT CCAGGACGAT GCAGTTGTGA TTCTATGCAG 
TCTGTACAAT GCTACAGGCT CATGGAACTC CCATCAGGCA TTCCTTCCAC CACCAAGAGA 
CTCTACATTA GCCACAGCAG AATCCAGCAC CTTCAGCTAT CTAACTTCAC TGGACTGTTG 
GCTCTGGAAG ATTTTATTCT GCTGGCCAGT GGAACAGAGT CTATTGAAAA TGACACCTTC 
AAAACTCTGA GTACACTAAA GACTCTAGAA CTTTGGAAAA ATAAATTGAG ACAAGTCCCC 
AGTGCTCTCC CAGCCAACCT TGAGGTGTTA AAATTAAATG ATAATGCCAT ATGTGCTCTA 
CGTGGATCAG AGTTTGAAGG ACTGAAGAAC TTGAAAGTCC TTGAACTTAA AAACAACTTG 
ATCTCGTCCC TGTCTCCCAG CATGCTCTCC CCACTTGCCA GTTTGCAGAG TTTGATGGTG 
GATGGTAACA ACATAGAGTC CGTGGTTGGA CCTCTGAGCC TTCCGCATCT CAAGTACATG 
AGCATGGAGA ACAACCAACT CCACCTTATA CCAGGGAATG TCTTCACTTC CCTACAGAAT 
TTGCAGTTCC TCAGTTTCAG TGGTAATTTT CTAACTAAAA TTCCCATAAA TCTACCCAAA 
TCCTTGCTAT CTTTAAAGAT GGAGAGAAAC CAGCTCAAAG TGGTCAGGTT TCGCGATATG 
AAACACTTGG AGAACCTGTC CCATCTTTAC CTGTCAGAGA ACTTCCTGTC TTCCATTGAT 
GGGGCACAGC AGCTGACCAA TTTAACAACT CTGGAGGTTT CCCAAAACCA GCTTCAAATG 
TTGCCTCCCA GGCTACCATC GAGGTTACAG AAGCTTGATT GTAGCAGTAA CTTCATTCAG 
AGAGTGACAG CACCAGAATT CCAGGACCTC CGAGACCTGA AACATCTGTT TCTAGACAAC 
AATGTTGTCA GCTTGTTCGA GGCTGGGGCC CTTCAGAGGT GTTCTCAGCT GTCCAACCTG 
GCCCTGGAGC AGAATCTGCT GTTGTCTATC CCTCTGAGGC TCCCTAAGAC CCTGGCCAGG 
CTGGACCTCA AGGGCAATGC CATCCAGGAC ATGGCTGAAA GGGAGCTCAG GGACCTCAAA 
CAACTGCAGG TCCTCAACCT CAGGAACAAC CGGATCTCGG CCTTAGATTT CAAGGCACTG 
GAGGGGCTGC CTCGGCTCAG GCATCTCTAC CTGGATGGGA ATCCCTGGAA CTGCACTTGC 
AGTCTCCTAC GAGCCAGGGA AGTCCTGAAG GCCAAGGGCA CGGACGTGAA AGGGGGACAG 
TGCGCAGCCC CAGCAGAGCG ACAGGGGGAG AGCTGGATGT CCTCCAAGAA GATCCTGAGG 
CAATGTGAGC ATCACTTACA GCAGAGTGAG AAAAGCAAAG AGACCAAGAA GAAACCCAAA 
CCCGAAGACT CTTCCAGCAT CAGACTCAAC ATGGATGACG ACGATGACGA TTATGAAATA 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512881.1
CDS	268..1953
Translation	MEQMELPPTPCLLTRTSTHSKDSVIRLRGIKSKVSPEQLLATCKPLCWTMHPLWAFLLGLSLTNGLSANCPGRCSCDSMQSVQCYRLMELPSGIPSTTKRLYISHSRIQHLQLSNFTGLLALEDFILLASGTESIENDTFKTLSTLKTLELWKNKLRQVPSALPANLEVLKLNDNAICALRGSEFEGLKNLKVLELKNNLISSLSPSMLSPLASLQSLMVDGNNIESVVGPLSLPHLKYMSMENNQLHLIPGNVFTSLQNLQFLSFSGNFLTKIPINLPKSLLSLKMERNQLKVVRFRDMKHLENLSHLYLSENFLSSIDGAQQLTNLTTLEVSQNQLQMLPPRLPSRLQKLDCSSNFIQRVTAPEFQDLRDLKHLFLDNNVVSLFEAGALQRCSQLSNLALEQNLLLSIPLRLPKTLARLDLKGNAIQDMAERELRDLKQLQVLNLRNNRISALDFKALEGLPRLRHLYLDGNPWNCTCSLLRAREVLKAKGTDVKGGQCAAPAERQGESWMSSKKILRQCEHHLQQSEKSKETKKKPKPEDSSSIRLNMDDDDDDYEID

Target ORF information:

RefSeq Version	XM_006512818.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nephrocan (Nepn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512818.4

ORF Insert Sequence:

ATGGAACAAA TGGAGTTACC CCCAACCCCC TGTCTTCTCA CAAGGACAAG CACACATTCA 
AAAGATTCTG TTATCAGACT CCGTGGTATA AAAAGTAAGG TGTCACCTGA ACAGTTGCTT 
GCTACTTGCA AACCTCTGTG TTGGACAATG CACCCGCTTT GGGCTTTTCT TTTGGGGCTC 
TCTCTTACCA ATGGCCTCAG TGCAAACTGT CCAGGACGAT GCAGTTGTGA TTCTATGCAG 
TCTGTACAAT GCTACAGGCT CATGGAACTC CCATCAGGCA TTCCTTCCAC CACCAAGAGA 
CTCTACATTA GCCACAGCAG AATCCAGCAC CTTCAGCTAT CTAACTTCAC TGGACTGTTG 
GCTCTGGAAG ATTTTATTCT GCTGGCCAGT GGAACAGAGT CTATTGAAAA TGACACCTTC 
AAAACTCTGA GTACACTAAA GACTCTAGAA CTTTGGAAAA ATAAATTGAG ACAAGTCCCC 
AGTGCTCTCC CAGCCAACCT TGAGGTGTTA AAATTAAATG ATAATGCCAT ATGTGCTCTA 
CGTGGATCAG AGTTTGAAGG ACTGAAGAAC TTGAAAGTCC TTGAACTTAA AAACAACTTG 
ATCTCGTCCC TGTCTCCCAG CATGCTCTCC CCACTTGCCA GTTTGCAGAG TTTGATGGTG 
GATGGTAACA ACATAGAGTC CGTGGTTGGA CCTCTGAGCC TTCCGCATCT CAAGTACATG 
AGCATGGAGA ACAACCAACT CCACCTTATA CCAGGGAATG TCTTCACTTC CCTACAGAAT 
TTGCAGTTCC TCAGTTTCAG TGGTAATTTT CTAACTAAAA TTCCCATAAA TCTACCCAAA 
TCCTTGCTAT CTTTAAAGAT GGAGAGAAAC CAGCTCAAAG TGGTCAGGTT TCGCGATATG 
AAACACTTGG AGAACCTGTC CCATCTTTAC CTGTCAGAGA ACTTCCTGTC TTCCATTGAT 
GGGGCACAGC AGCTGACCAA TTTAACAACT CTGGAGGTTT CCCAAAACCA GCTTCAAATG 
TTGCCTCCCA GGCTACCATC GAGGTTACAG AAGCTTGATT GTAGCAGTAA CTTCATTCAG 
AGAGTGACAG CACCAGAATT CCAGGACCTC CGAGACCTGA AACATCTGTT TCTAGACAAC 
AATGTTGTCA GCTTGTTCGA GGCTGGGGCC CTTCAGAGGT GTTCTCAGCT GTCCAACCTG 
GCCCTGGAGC AGAATCTGCT GTTGTCTATC CCTCTGAGGC TCCCTAAGAC CCTGGCCAGG 
CTGGACCTCA AGGGCAATGC CATCCAGGAC ATGGCTGAAA GGGAGCTCAG GGACCTCAAA 
CAACTGCAGG TCCTCAACCT CAGGAACAAC CGGATCTCGG CCTTAGATTT CAAGGCACTG 
GAGGGGCTGC CTCGGCTCAG GCATCTCTAC CTGGATGGGA ATCCCTGGAA CTGCACTTGC 
AGTCTCCTAC GAGCCAGGGA AGTCCTGAAG GCCAAGGGCA CGGACGTGAA AGGGGGACAG 
TGCGCAGCCC CAGCAGAGCG ACAGGGGGAG AGCTGGATGT CCTCCAAGAA GATCCTGAGG 
CAATGTGAGC ATCACTTACA GCAGAGTGAG AAAAGCAAAG AGACCAAGAA GAAACCCAAA 
CCCGAAGACT CTTCCAGCAT CAGACTCAAC ATGGATGACG ACGATGACGA TTATGAAATA 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nepn ( XM_006512818.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512881.1 Mus musculus nephrocan (Nepn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43928
Clone ID Related Accession (Same CDS sequence)	XM_006512818.4 , XM_006512818.3
Accession Version	XM_006512818.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	nephrocan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512818.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAA TGGAGTTACC CCCAACCCCC TGTCTTCTCA CAAGGACAAG CACACATTCA 
AAAGATTCTG TTATCAGACT CCGTGGTATA AAAAGTAAGG TGTCACCTGA ACAGTTGCTT 
GCTACTTGCA AACCTCTGTG TTGGACAATG CACCCGCTTT GGGCTTTTCT TTTGGGGCTC 
TCTCTTACCA ATGGCCTCAG TGCAAACTGT CCAGGACGAT GCAGTTGTGA TTCTATGCAG 
TCTGTACAAT GCTACAGGCT CATGGAACTC CCATCAGGCA TTCCTTCCAC CACCAAGAGA 
CTCTACATTA GCCACAGCAG AATCCAGCAC CTTCAGCTAT CTAACTTCAC TGGACTGTTG 
GCTCTGGAAG ATTTTATTCT GCTGGCCAGT GGAACAGAGT CTATTGAAAA TGACACCTTC 
AAAACTCTGA GTACACTAAA GACTCTAGAA CTTTGGAAAA ATAAATTGAG ACAAGTCCCC 
AGTGCTCTCC CAGCCAACCT TGAGGTGTTA AAATTAAATG ATAATGCCAT ATGTGCTCTA 
CGTGGATCAG AGTTTGAAGG ACTGAAGAAC TTGAAAGTCC TTGAACTTAA AAACAACTTG 
ATCTCGTCCC TGTCTCCCAG CATGCTCTCC CCACTTGCCA GTTTGCAGAG TTTGATGGTG 
GATGGTAACA ACATAGAGTC CGTGGTTGGA CCTCTGAGCC TTCCGCATCT CAAGTACATG 
AGCATGGAGA ACAACCAACT CCACCTTATA CCAGGGAATG TCTTCACTTC CCTACAGAAT 
TTGCAGTTCC TCAGTTTCAG TGGTAATTTT CTAACTAAAA TTCCCATAAA TCTACCCAAA 
TCCTTGCTAT CTTTAAAGAT GGAGAGAAAC CAGCTCAAAG TGGTCAGGTT TCGCGATATG 
AAACACTTGG AGAACCTGTC CCATCTTTAC CTGTCAGAGA ACTTCCTGTC TTCCATTGAT 
GGGGCACAGC AGCTGACCAA TTTAACAACT CTGGAGGTTT CCCAAAACCA GCTTCAAATG 
TTGCCTCCCA GGCTACCATC GAGGTTACAG AAGCTTGATT GTAGCAGTAA CTTCATTCAG 
AGAGTGACAG CACCAGAATT CCAGGACCTC CGAGACCTGA AACATCTGTT TCTAGACAAC 
AATGTTGTCA GCTTGTTCGA GGCTGGGGCC CTTCAGAGGT GTTCTCAGCT GTCCAACCTG 
GCCCTGGAGC AGAATCTGCT GTTGTCTATC CCTCTGAGGC TCCCTAAGAC CCTGGCCAGG 
CTGGACCTCA AGGGCAATGC CATCCAGGAC ATGGCTGAAA GGGAGCTCAG GGACCTCAAA 
CAACTGCAGG TCCTCAACCT CAGGAACAAC CGGATCTCGG CCTTAGATTT CAAGGCACTG 
GAGGGGCTGC CTCGGCTCAG GCATCTCTAC CTGGATGGGA ATCCCTGGAA CTGCACTTGC 
AGTCTCCTAC GAGCCAGGGA AGTCCTGAAG GCCAAGGGCA CGGACGTGAA AGGGGGACAG 
TGCGCAGCCC CAGCAGAGCG ACAGGGGGAG AGCTGGATGT CCTCCAAGAA GATCCTGAGG 
CAATGTGAGC ATCACTTACA GCAGAGTGAG AAAAGCAAAG AGACCAAGAA GAAACCCAAA 
CCCGAAGACT CTTCCAGCAT CAGACTCAAC ATGGATGACG ACGATGACGA TTATGAAATA 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512881.1
CDS	268..1953
Translation	MEQMELPPTPCLLTRTSTHSKDSVIRLRGIKSKVSPEQLLATCKPLCWTMHPLWAFLLGLSLTNGLSANCPGRCSCDSMQSVQCYRLMELPSGIPSTTKRLYISHSRIQHLQLSNFTGLLALEDFILLASGTESIENDTFKTLSTLKTLELWKNKLRQVPSALPANLEVLKLNDNAICALRGSEFEGLKNLKVLELKNNLISSLSPSMLSPLASLQSLMVDGNNIESVVGPLSLPHLKYMSMENNQLHLIPGNVFTSLQNLQFLSFSGNFLTKIPINLPKSLLSLKMERNQLKVVRFRDMKHLENLSHLYLSENFLSSIDGAQQLTNLTTLEVSQNQLQMLPPRLPSRLQKLDCSSNFIQRVTAPEFQDLRDLKHLFLDNNVVSLFEAGALQRCSQLSNLALEQNLLLSIPLRLPKTLARLDLKGNAIQDMAERELRDLKQLQVLNLRNNRISALDFKALEGLPRLRHLYLDGNPWNCTCSLLRAREVLKAKGTDVKGGQCAAPAERQGESWMSSKKILRQCEHHLQQSEKSKETKKKPKPEDSSSIRLNMDDDDDDYEID

Target ORF information:

RefSeq Version	XM_006512818.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nephrocan (Nepn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512818.3

ORF Insert Sequence:

ATGGAACAAA TGGAGTTACC CCCAACCCCC TGTCTTCTCA CAAGGACAAG CACACATTCA 
AAAGATTCTG TTATCAGACT CCGTGGTATA AAAAGTAAGG TGTCACCTGA ACAGTTGCTT 
GCTACTTGCA AACCTCTGTG TTGGACAATG CACCCGCTTT GGGCTTTTCT TTTGGGGCTC 
TCTCTTACCA ATGGCCTCAG TGCAAACTGT CCAGGACGAT GCAGTTGTGA TTCTATGCAG 
TCTGTACAAT GCTACAGGCT CATGGAACTC CCATCAGGCA TTCCTTCCAC CACCAAGAGA 
CTCTACATTA GCCACAGCAG AATCCAGCAC CTTCAGCTAT CTAACTTCAC TGGACTGTTG 
GCTCTGGAAG ATTTTATTCT GCTGGCCAGT GGAACAGAGT CTATTGAAAA TGACACCTTC 
AAAACTCTGA GTACACTAAA GACTCTAGAA CTTTGGAAAA ATAAATTGAG ACAAGTCCCC 
AGTGCTCTCC CAGCCAACCT TGAGGTGTTA AAATTAAATG ATAATGCCAT ATGTGCTCTA 
CGTGGATCAG AGTTTGAAGG ACTGAAGAAC TTGAAAGTCC TTGAACTTAA AAACAACTTG 
ATCTCGTCCC TGTCTCCCAG CATGCTCTCC CCACTTGCCA GTTTGCAGAG TTTGATGGTG 
GATGGTAACA ACATAGAGTC CGTGGTTGGA CCTCTGAGCC TTCCGCATCT CAAGTACATG 
AGCATGGAGA ACAACCAACT CCACCTTATA CCAGGGAATG TCTTCACTTC CCTACAGAAT 
TTGCAGTTCC TCAGTTTCAG TGGTAATTTT CTAACTAAAA TTCCCATAAA TCTACCCAAA 
TCCTTGCTAT CTTTAAAGAT GGAGAGAAAC CAGCTCAAAG TGGTCAGGTT TCGCGATATG 
AAACACTTGG AGAACCTGTC CCATCTTTAC CTGTCAGAGA ACTTCCTGTC TTCCATTGAT 
GGGGCACAGC AGCTGACCAA TTTAACAACT CTGGAGGTTT CCCAAAACCA GCTTCAAATG 
TTGCCTCCCA GGCTACCATC GAGGTTACAG AAGCTTGATT GTAGCAGTAA CTTCATTCAG 
AGAGTGACAG CACCAGAATT CCAGGACCTC CGAGACCTGA AACATCTGTT TCTAGACAAC 
AATGTTGTCA GCTTGTTCGA GGCTGGGGCC CTTCAGAGGT GTTCTCAGCT GTCCAACCTG 
GCCCTGGAGC AGAATCTGCT GTTGTCTATC CCTCTGAGGC TCCCTAAGAC CCTGGCCAGG 
CTGGACCTCA AGGGCAATGC CATCCAGGAC ATGGCTGAAA GGGAGCTCAG GGACCTCAAA 
CAACTGCAGG TCCTCAACCT CAGGAACAAC CGGATCTCGG CCTTAGATTT CAAGGCACTG 
GAGGGGCTGC CTCGGCTCAG GCATCTCTAC CTGGATGGGA ATCCCTGGAA CTGCACTTGC 
AGTCTCCTAC GAGCCAGGGA AGTCCTGAAG GCCAAGGGCA CGGACGTGAA AGGGGGACAG 
TGCGCAGCCC CAGCAGAGCG ACAGGGGGAG AGCTGGATGT CCTCCAAGAA GATCCTGAGG 
CAATGTGAGC ATCACTTACA GCAGAGTGAG AAAAGCAAAG AGACCAAGAA GAAACCCAAA 
CCCGAAGACT CTTCCAGCAT CAGACTCAAC ATGGATGACG ACGATGACGA TTATGAAATA 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A regulatory network controls nephrocan expression and midgut patterning.
Development (Cambridge, England)141(19)3772-81(2014 Oct)
Hou J,Wei W,Saund RS,Xiang P,Cunningham TJ,Yi Y,Alder O,Lu DY,Savory JG,Krentz NA,Montpetit R,Cullum R,Hofs N,Lohnes D,Humphries RK,Yamanaka Y,Duester G,Saijoh Y,Hoodless PA

Loss of function of mouse Pax-Interacting Protein 1-associated glutamate rich protein 1a (Pagr1a) leads to reduced Bmp2 expression and defects in chorion and amnion development.
Developmental dynamics : an official publication of the American Association of Anatomists243(7)937-47(2014 Jul)
Kumar A,Lualdi M,Loncarek J,Cho YW,Lee JE,Ge K,Kuehn MR

miR-335 promotes mesendodermal lineage segregation and shapes a transcription factor gradient in the endoderm.
Development (Cambridge, England)141(3)514-25(2014 Feb)
Yang D,Lutter D,Burtscher I,Uetzmann L,Theis FJ,Lickert H

Wnt/β-catenin signalling regulates Sox17 expression and is essential for organizer and endoderm formation in the mouse.
Development (Cambridge, England)140(15)3128-38(2013 Aug)
Engert S,Burtscher I,Liao WP,Dulev S,Schotta G,Lickert H

Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord.
Developmental biology360(2)415-25(2011 Dec)
Tamplin OJ,Cox BJ,Rossant J

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Nepn cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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