Trpm5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trpm5
Entrez Gene ID	56843
Full Name	transient receptor potential cation channel, subfamily M, member 5
Synonyms	9430099A16Rik,LTrpC-5,Ltrpc5,Mtr1
General protein information	Preferred Names transient receptor potential cation channel, subfamily M, member 5 Names transient receptor potential cation channel subfamily M member 5 MLSN1- and TRP-related gene 1 protein long transient receptor potential channel 5 long transient receptor potential-related channel 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F5

mRNA and Protein(s)

mRNA	Protein	Name
XM_017322383.1	XP_017177872.1	transient receptor potential cation channel subfamily M member 5 isoform X3
XM_006508646.4	XP_006508709.1	transient receptor potential cation channel subfamily M member 5 isoform X2
XM_006508645.1	XP_006508708.1	transient receptor potential cation channel subfamily M member 5 isoform X1
XM_006508646.3	XP_006508709.1	transient receptor potential cation channel subfamily M member 5 isoform X2
NM_020277.2	NP_064673.2	transient receptor potential cation channel subfamily M member 5
XM_017322384.1	XP_017177873.1	transient receptor potential cation channel subfamily M member 5 isoform X4
XM_030242820.1	XP_030098680.1	transient receptor potential cation channel subfamily M member 5 isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Trpm5	NP_064673.2
Pan troglodytes (chimpanzee)	TRPM5	XP_521720.4
Rattus norvegicus (Norway rat)	Trpm5	NP_001178825.1
Homo sapiens (human)	TRPM5	NP_055370.1
Macaca mulatta (Rhesus monkey)	TRPM5	XP_001093334.1
Gallus gallus (chicken)	TRPM5	XP_003641369.2
Bos taurus (cattle)	TRPM5	XP_002699492.2
Canis lupus familiaris (dog)	TRPM5	XP_005631408.1
Danio rerio (zebrafish)	trpm5	NP_001121711.1

Gene Ontology
Bibliography

Related articles in PubMed

Impairment of Bitter Taste Sensor Transient Receptor Potential Channel M5-Mediated Aversion Aggravates High-Salt Intake and Hypertension.
Cui Y, Wu H, Li Q, Liao J, Gao P, Sun F, Zhang H, Lu Z, Wei X, He C, Ma T, Wei X, Chen X, Zheng H, Yang G, Liu D, Zhu Z
Hypertension (Dallas, Tex. : 1979)74(4)1021-1032(2019 10)

A Metabolite-Triggered Tuft Cell-ILC2 Circuit Drives Small Intestinal Remodeling.
Schneider C, O'Leary CE, von Moltke J, Liang HE, Ang QY, Turnbaugh PJ, Radhakrishnan S, Pellizzon M, Ma A, Locksley RM
Cell174(2)271-284.e14(2018 07)

Detection of Succinate by Intestinal Tuft Cells Triggers a Type 2 Innate Immune Circuit.
Nadjsombati MS, McGinty JW, Lyons-Cohen MR, Jaffe JB, DiPeso L, Schneider C, Miller CN, Pollack JL, Nagana Gowda GA, Fontana MF, Erle DJ, Anderson MS, Locksley RM, Raftery D, von Moltke J
Immunity49(1)33-41.e7(2018 07)

Thymic tuft cells promote an IL-4-enriched medulla and shape thymocyte development.
Miller CN, Proekt I, von Moltke J, Wells KL, Rajpurkar AR, Wang H, Rattay K, Khan IS, Metzger TC, Pollack JL, Fries AC, Lwin WW, Wigton EJ, Parent AV, Kyewski B, Erle DJ, Hogquist KA, Steinmetz LM, Locksley RM, Anderson MS
Nature559(7715)627-631(2018 07)

Chemical synapses without synaptic vesicles: Purinergic neurotransmission through a CALHM1 channel-mitochondrial signaling complex.
Romanov RA, Lasher RS, High B, Savidge LE, Lawson A, Rogachevskaja OA, Zhao H, Rogachevsky VV, Bystrova MF, Churbanov GD, Adameyko I, Harkany T, Yang R, Kidd GJ, Marambaud P, Kinnamon JC, Kolesnikov SS, Finger TE
Science signaling11(529)(2018 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Trpm5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trpm5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu82155	XM_017322383.1 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu82156	XM_017322384.1 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu82156	XM_030242820.1 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu18787	NM_020277.2 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu18787	XM_006508645.1 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu40285	XM_006508646.4 Latest version!	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu40285	XM_006508646.3	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trpm5 ( XM_017322383.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177872.1 Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu82155
Clone ID Related Accession (Same CDS sequence)	XM_017322383.1
Accession Version	XM_017322383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACAA CCCAGAGCTC CTGCCCCGGC AGCCCCCCAG ATACTGAGGA TGGCTGGGAG 
CCCATCCTAT GCAGGGGAGA GATCAACTTC GGAGGGTCTG GGAAGAAGCG AGGCAAGTTT 
GTGAAGGTGC CAAGCAGTGT GGCCCCCTCC GTGCTTTTTG AACTCCTGCT CACCGAGTGG 
CACCTGCCAG CCCCCAACCT GGTGGTGTCC CTGGTGGGTG AGGAACGACC TTTGGCTATG 
AAGTCGTGGC TTCGGGATGT CCTGCGCAAG GGGCTGGTGA AAGCAGCTCA GAGCACAGGT 
GCCTGGATCC TGACCAGTGC CCTCCACGTG GGCCTGGCCC GCCATGTTGG ACAAGCTGTA 
CGTGATCACT CTCTGGCTAG CACATCCACC AAGATCCGTG TAGTGGCCAT CGGAATGGCC 
TCTCTGGATC GAATCCTTCA CCGTCAACTT CTAGATGGTG TCCACCAAAA GGAGGATACT 
CCCATCCACT ACCCAGCAGA TGAGGGCAAC ATTCAGGGAC CCCTCTGCCC CCTGGACAGC 
AATCTCTCCC ACTTCATCCT TGTGGAGTCA GGCGCCCTTG GGAGTGGGAA CGACGGGCTG 
ACAGAGCTGC AGCTGAGCCT GGAGAAGCAC ATCTCTCAGC AGAGGACAGG TTATGGGGGC 
ACCAGCTGCA TCCAGATACC TGTCCTTTGC CTGTTGGTCA ATGGTGACCC CAACACCCTA 
GAGAGGATTT CCAGGGCAGT GGAGCAGGCT GCCCCATGGC TGATCCTGGC AGGTTCTGGT 
GGCATTGCTG ATGTACTCGC TGCCCTGGTG AGCCAGCCTC ATCTCCTGGT GCCCCAGGTG 
GCTGAGAAGC AGTTCAGAGA GAAATTCCCC AGCGAGTGTT TCTCTTGGGA AGCCATTGTA 
CACTGGACAG AGCTGTTACA GAACATTGCT GCACACCCCC ACCTGCTCAC AGTATATGAC 
TTCGAGCAGG AGGGTTCGGA GGACCTGGAC ACTGTCATCC TCAAGGCACT TGTGAAAGCC 
TGCAAGAGCC ACAGCCAAGA AGCCCAAGAC TACCTAGATG AGCTCAAGTT AGCAGTGGCC 
TGGGATCGCG TGGACATTGC CAAGAGTGAA ATCTTCAATG GGGACGTGGA ATGGAAGTCC 
TGTGACTTGG AAGAGGTGAT GACAGATGCC CTCGTGAGCA ACAAGCCTGA CTTTGTCCGC 
CTCTTTGTGG ACAGCGGTGC TGACATGGCC GAGTTCTTGA CCTATGGGCG GCTGCAGCAG 
CTTTACCATT CTGTGTCCCC CAAGAGCCTC CTCTTTGAAC TGCTGCAGCG TAAGCATGAG 
GAGGGTAGGC TGACACTGGC CGGCCTGGGT GCCCAGCAGG CTCGGGAGCT GCCCATTGGT 
CTGCCTGCCT TCTCACTCCA CGAGGTCTCC CGCGTACTCA AAGACTTCCT GCATGACGCC 
TGCCGTGGCT TCTACCAGGA CGGGCGCAGG ATGGAGGAGA GAGGGCCACC TAAGCGGCCC 
GCAGGCCAGA AGTGGCTGCC AGACCTCAGT AGGAAGAGTG AAGACCCTTG GAGGGACCTG 
TTCCTCTGGG CTGTGCTGCA GAATCGTTAT GAGATGGCCA CATACTTCTG GGCCATGGGC 
CGGGAGGGTG TGGCTGCTGC TCTGGCTGCC TGCAAGATCA TAAAGGAAAT GTCCCACCTG 
GAGAAAGAGG CAGAGGTGGC CCGCACCATG CGTGAGGCCA AGTATGAGCA GCTGGCCCTG 
GATCTTTTCT CAGAGTGCTA CGGCAACAGT GAGGACCGTG CCTTTGCCCT GCTGGTGCGA 
AGGAACCACA GCTGGAGCAG GACCACGTGC CTGCACCTGG CCACTGAAGC TGATGCCAAG 
GCCTTCTTTG CCCATGACGG TGTGCAAGCA TTCCTGACCA AGATCTGGTG GGGAGACATG 
GCCACAGGCA CACCCATCCT ACGGCTTCTG GGTGCCTTCA CCTGCCCAGC CCTCATCTAC 
ACAAACCTCA TCTCCTTCAG TGAGGATGCC CCGCAGAGGA TGGACCTAGA AGATCTGCAG 
GAGCCAGACA GCTTGGATAT GGAAAAGAGC TTCCTATGCA GCCGGGGTGG CCAATTGGAG 
AAGCTAACAG AGGCACCAAG GGCTCCAGGC GATCTAGGCC CACAAGCTGC CTTCCTGCTC 
ACACGGTGGA GGAAGTTCTG GGGCGCTCCT GTGACTGTGT TCCTGGGGAA TGTGGTCATG 
TACTTCGCAT TCCTCTTCCT GTTCACCTAT GTCCTGCTGG TGGACTTCAG GCCACCACCC 
CAGGGGCCGT CTGGATCCGA GGTTACCCTC TATTTCTGGG TGTTCACACT GGTGCTGGAG 
GAAATCCGAC AGGGCTTCTT CACAGATGAG GACACGCACC TGGTGAAGAA ATTCACTCTG 
TATGTGGAAG ACAACTGGAA CAAGTGTGAC ATGGTGGCCA TCTTCCTGTT CATTGTGGGA 
GTCACCTGTA GAGGCTCGTG TGAACTGTTC TCTTCACCCT CTGCTGCTGG AAAGCTCGGC 
TTCCTGCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177872.1
CDS	64..2595
Translation	MQTTQSSCPGSPPDTEDGWEPILCRGEINFGGSGKKRGKFVKVPSSVAPSVLFELLLTEWHLPAPNLVVSLVGEERPLAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKIRVVAIGMASLDRILHRQLLDGVHQKEDTPIHYPADEGNIQGPLCPLDSNLSHFILVESGALGSGNDGLTELQLSLEKHISQQRTGYGGTSCIQIPVLCLLVNGDPNTLERISRAVEQAAPWLILAGSGGIADVLAALVSQPHLLVPQVAEKQFREKFPSECFSWEAIVHWTELLQNIAAHPHLLTVYDFEQEGSEDLDTVILKALVKACKSHSQEAQDYLDELKLAVAWDRVDIAKSEIFNGDVEWKSCDLEEVMTDALVSNKPDFVRLFVDSGADMAEFLTYGRLQQLYHSVSPKSLLFELLQRKHEEGRLTLAGLGAQQARELPIGLPAFSLHEVSRVLKDFLHDACRGFYQDGRRMEERGPPKRPAGQKWLPDLSRKSEDPWRDLFLWAVLQNRYEMATYFWAMGREGVAAALAACKIIKEMSHLEKEAEVARTMREAKYEQLALDLFSECYGNSEDRAFALLVRRNHSWSRTTCLHLATEADAKAFFAHDGVQAFLTKIWWGDMATGTPILRLLGAFTCPALIYTNLISFSEDAPQRMDLEDLQEPDSLDMEKSFLCSRGGQLEKLTEAPRAPGDLGPQAAFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFTYVLLVDFRPPPQGPSGSEVTLYFWVFTLVLEEIRQGFFTDEDTHLVKKFTLYVEDNWNKCDMVAIFLFIVGVTCRGSCELFSSPSAAGKLGFLP

Target ORF information:

RefSeq Version	XM_017322383.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322383.1

ORF Insert Sequence:

ATGCAAACAA CCCAGAGCTC CTGCCCCGGC AGCCCCCCAG ATACTGAGGA TGGCTGGGAG 
CCCATCCTAT GCAGGGGAGA GATCAACTTC GGAGGGTCTG GGAAGAAGCG AGGCAAGTTT 
GTGAAGGTGC CAAGCAGTGT GGCCCCCTCC GTGCTTTTTG AACTCCTGCT CACCGAGTGG 
CACCTGCCAG CCCCCAACCT GGTGGTGTCC CTGGTGGGTG AGGAACGACC TTTGGCTATG 
AAGTCGTGGC TTCGGGATGT CCTGCGCAAG GGGCTGGTGA AAGCAGCTCA GAGCACAGGT 
GCCTGGATCC TGACCAGTGC CCTCCACGTG GGCCTGGCCC GCCATGTTGG ACAAGCTGTA 
CGTGATCACT CTCTGGCTAG CACATCCACC AAGATCCGTG TAGTGGCCAT CGGAATGGCC 
TCTCTGGATC GAATCCTTCA CCGTCAACTT CTAGATGGTG TCCACCAAAA GGAGGATACT 
CCCATCCACT ACCCAGCAGA TGAGGGCAAC ATTCAGGGAC CCCTCTGCCC CCTGGACAGC 
AATCTCTCCC ACTTCATCCT TGTGGAGTCA GGCGCCCTTG GGAGTGGGAA CGACGGGCTG 
ACAGAGCTGC AGCTGAGCCT GGAGAAGCAC ATCTCTCAGC AGAGGACAGG TTATGGGGGC 
ACCAGCTGCA TCCAGATACC TGTCCTTTGC CTGTTGGTCA ATGGTGACCC CAACACCCTA 
GAGAGGATTT CCAGGGCAGT GGAGCAGGCT GCCCCATGGC TGATCCTGGC AGGTTCTGGT 
GGCATTGCTG ATGTACTCGC TGCCCTGGTG AGCCAGCCTC ATCTCCTGGT GCCCCAGGTG 
GCTGAGAAGC AGTTCAGAGA GAAATTCCCC AGCGAGTGTT TCTCTTGGGA AGCCATTGTA 
CACTGGACAG AGCTGTTACA GAACATTGCT GCACACCCCC ACCTGCTCAC AGTATATGAC 
TTCGAGCAGG AGGGTTCGGA GGACCTGGAC ACTGTCATCC TCAAGGCACT TGTGAAAGCC 
TGCAAGAGCC ACAGCCAAGA AGCCCAAGAC TACCTAGATG AGCTCAAGTT AGCAGTGGCC 
TGGGATCGCG TGGACATTGC CAAGAGTGAA ATCTTCAATG GGGACGTGGA ATGGAAGTCC 
TGTGACTTGG AAGAGGTGAT GACAGATGCC CTCGTGAGCA ACAAGCCTGA CTTTGTCCGC 
CTCTTTGTGG ACAGCGGTGC TGACATGGCC GAGTTCTTGA CCTATGGGCG GCTGCAGCAG 
CTTTACCATT CTGTGTCCCC CAAGAGCCTC CTCTTTGAAC TGCTGCAGCG TAAGCATGAG 
GAGGGTAGGC TGACACTGGC CGGCCTGGGT GCCCAGCAGG CTCGGGAGCT GCCCATTGGT 
CTGCCTGCCT TCTCACTCCA CGAGGTCTCC CGCGTACTCA AAGACTTCCT GCATGACGCC 
TGCCGTGGCT TCTACCAGGA CGGGCGCAGG ATGGAGGAGA GAGGGCCACC TAAGCGGCCC 
GCAGGCCAGA AGTGGCTGCC AGACCTCAGT AGGAAGAGTG AAGACCCTTG GAGGGACCTG 
TTCCTCTGGG CTGTGCTGCA GAATCGTTAT GAGATGGCCA CATACTTCTG GGCCATGGGC 
CGGGAGGGTG TGGCTGCTGC TCTGGCTGCC TGCAAGATCA TAAAGGAAAT GTCCCACCTG 
GAGAAAGAGG CAGAGGTGGC CCGCACCATG CGTGAGGCCA AGTATGAGCA GCTGGCCCTG 
GATCTTTTCT CAGAGTGCTA CGGCAACAGT GAGGACCGTG CCTTTGCCCT GCTGGTGCGA 
AGGAACCACA GCTGGAGCAG GACCACGTGC CTGCACCTGG CCACTGAAGC TGATGCCAAG 
GCCTTCTTTG CCCATGACGG TGTGCAAGCA TTCCTGACCA AGATCTGGTG GGGAGACATG 
GCCACAGGCA CACCCATCCT ACGGCTTCTG GGTGCCTTCA CCTGCCCAGC CCTCATCTAC 
ACAAACCTCA TCTCCTTCAG TGAGGATGCC CCGCAGAGGA TGGACCTAGA AGATCTGCAG 
GAGCCAGACA GCTTGGATAT GGAAAAGAGC TTCCTATGCA GCCGGGGTGG CCAATTGGAG 
AAGCTAACAG AGGCACCAAG GGCTCCAGGC GATCTAGGCC CACAAGCTGC CTTCCTGCTC 
ACACGGTGGA GGAAGTTCTG GGGCGCTCCT GTGACTGTGT TCCTGGGGAA TGTGGTCATG 
TACTTCGCAT TCCTCTTCCT GTTCACCTAT GTCCTGCTGG TGGACTTCAG GCCACCACCC 
CAGGGGCCGT CTGGATCCGA GGTTACCCTC TATTTCTGGG TGTTCACACT GGTGCTGGAG 
GAAATCCGAC AGGGCTTCTT CACAGATGAG GACACGCACC TGGTGAAGAA ATTCACTCTG 
TATGTGGAAG ACAACTGGAA CAAGTGTGAC ATGGTGGCCA TCTTCCTGTT CATTGTGGGA 
GTCACCTGTA GAGGCTCGTG TGAACTGTTC TCTTCACCCT CTGCTGCTGG AAAGCTCGGC 
TTCCTGCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm5 ( XM_017322384.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177873.1 Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu82156
Clone ID Related Accession (Same CDS sequence)	XM_017322384.1 , XM_030242820.1
Accession Version	XM_017322384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGATG CCCTCGTGAG CAACAAGCCT GACTTTGTCC GCCTCTTTGT GGACAGCGGT 
GCTGACATGG CCGAGTTCTT GACCTATGGG CGGCTGCAGC AGCTTTACCA TTCTGTGTCC 
CCCAAGAGCC TCCTCTTTGA ACTGCTGCAG CGTAAGCATG AGGAGGGTAG GCTGACACTG 
GCCGGCCTGG GTGCCCAGCA GGCTCGGGAG CTGCCCATTG GTCTGCCTGC CTTCTCACTC 
CACGAGGTCT CCCGCGTACT CAAAGACTTC CTGCATGACG CCTGCCGTGG CTTCTACCAG 
GACGGGCGCA GGATGGAGGA GAGAGGGCCA CCTAAGCGGC CCGCAGGCCA GAAGTGGCTG 
CCAGACCTCA GTAGGAAGAG TGAAGACCCT TGGAGGGACC TGTTCCTCTG GGCTGTGCTG 
CAGAATCGTT ATGAGATGGC CACATACTTC TGGGCCATGG GCCGGGAGGG TGTGGCTGCT 
GCTCTGGCTG CCTGCAAGAT CATAAAGGAA ATGTCCCACC TGGAGAAAGA GGCAGAGGTG 
GCCCGCACCA TGCGTGAGGC CAAGTATGAG CAGCTGGCCC TGGATCTTTT CTCAGAGTGC 
TACGGCAACA GTGAGGACCG TGCCTTTGCC CTGCTGGTGC GAAGGAACCA CAGCTGGAGC 
AGGACCACGT GCCTGCACCT GGCCACTGAA GCTGATGCCA AGGCCTTCTT TGCCCATGAC 
GGTGTGCAAG CATTCCTGAC CAAGATCTGG TGGGGAGACA TGGCCACAGG CACACCCATC 
CTACGGCTTC TGGGTGCCTT CACCTGCCCA GCCCTCATCT ACACAAACCT CATCTCCTTC 
AGTGAGGATG CCCCGCAGAG GATGGACCTA GAAGATCTGC AGGAGCCAGA CAGCTTGGAT 
ATGGAAAAGA GCTTCCTATG CAGCCGGGGT GGCCAATTGG AGAAGCTAAC AGAGGCACCA 
AGGGCTCCAG GCGATCTAGG CCCACAAGCT GCCTTCCTGC TCACACGGTG GAGGAAGTTC 
TGGGGCGCTC CTGTGACTGT GTTCCTGGGG AATGTGGTCA TGTACTTCGC ATTCCTCTTC 
CTGTTCACCT ATGTCCTGCT GGTGGACTTC AGGCCACCAC CCCAGGGGCC GTCTGGATCC 
GAGGTTACCC TCTATTTCTG GGTGTTCACA CTGGTGCTGG AGGAAATCCG ACAGGGCTTC 
TTCACAGATG AGGACACGCA CCTGGTGAAG AAATTCACTC TGTATGTGGA AGACAACTGG 
AACAAGTGTG ACATGGTGGC CATCTTCCTG TTCATTGTGG GAGTCACCTG TAGAATGGTG 
CCCTCGGTGT TTGAGGCTGG CAGGACCGTT CTGGCCATTG ACTTCATGGT GTTCACACTT 
CGGCTCATCC ACATCTTTGC TATTCACAAG CAGTTGGGTC CTAAGATCAT CATTGTAGAG 
CGAATGATGA AGGATGTCTT CTTTTTCCTC TTCTTCCTGA GCGTATGGCT TGTGGCCTAT 
GGTGTGACCA CTCAGGCCCT GCTGCATCCC CATGATGGCC GTTTGGAGTG GATTTTCCGC 
CGTGTGCTAT ACAGGCCTTA CCTGCAGATC TTTGGGCAAA TCCCTCTGGA TGAAATTGAT 
GAGGCTCGTG TGAACTGTTC TCTTCACCCT CTGCTGCTGG AAAGCTCGGC TTCCTGCCCT 
AATCTCTATG CCAACTGGCT GGTCATTCTC CTGCTGGTTA CCTTCCTGCT TGTCACTAAT 
GTGCTGCTCA TGAACCTTCT GATCGCCATG TTCAGCTACA CATTCCAGGT GGTGCAAGGC 
AATGCAGACA TGTTCTGGAA GTTTCAACGC TACCACCTCA TCGTTGAATA CCATGGAAGA 
CCAGCTCTGG CCCCGCCCTT CATCCTGCTC AGCCACCTGA GCCTGGTGCT CAAGCAGGTC 
TTCAGGAAGG AAGCCCAGCA TAAGCGACAA CATCTGGAGA GAGACTTGCC TGACCCCTTG 
GACCAGAAGA TCATTACCTG GGAAACGGTT CAAAAGGAGA ACTTCCTGAG TACCATGGAG 
AAACGGAGGA GGGACAGCGA GGGGGAGGTG CTGAGGAAAA CGGCACACAG AGTGGACTTG 
ATTGCCAAAT ACATCGGGGG GCTGAGAGAG CAAGAAAAGA GGATCAAGTG TCTGGAATCA 
CAGGCCAACT ACTGTATGCT CCTCTTGTCC TCTATGACGG ATACACTGGC TCCAGGAGGC 
ACCTACTCAA GCTCTCAGAA CTGTGGTTGC AGGAGTCAGC CAGCCTCTGC TAGAGACAGG 
GAGTACCTAG AGTCTGGCTT GCCACCCTCT GACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177873.1
CDS	41..2359
Translation	MTDALVSNKPDFVRLFVDSGADMAEFLTYGRLQQLYHSVSPKSLLFELLQRKHEEGRLTLAGLGAQQARELPIGLPAFSLHEVSRVLKDFLHDACRGFYQDGRRMEERGPPKRPAGQKWLPDLSRKSEDPWRDLFLWAVLQNRYEMATYFWAMGREGVAAALAACKIIKEMSHLEKEAEVARTMREAKYEQLALDLFSECYGNSEDRAFALLVRRNHSWSRTTCLHLATEADAKAFFAHDGVQAFLTKIWWGDMATGTPILRLLGAFTCPALIYTNLISFSEDAPQRMDLEDLQEPDSLDMEKSFLCSRGGQLEKLTEAPRAPGDLGPQAAFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFTYVLLVDFRPPPQGPSGSEVTLYFWVFTLVLEEIRQGFFTDEDTHLVKKFTLYVEDNWNKCDMVAIFLFIVGVTCRMVPSVFEAGRTVLAIDFMVFTLRLIHIFAIHKQLGPKIIIVERMMKDVFFFLFFLSVWLVAYGVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSLHPLLLESSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYHLIVEYHGRPALAPPFILLSHLSLVLKQVFRKEAQHKRQHLERDLPDPLDQKIITWETVQKENFLSTMEKRRRDSEGEVLRKTAHRVDLIAKYIGGLREQEKRIKCLESQANYCMLLLSSMTDTLAPGGTYSSSQNCGCRSQPASARDREYLESGLPPSDT

Target ORF information:

RefSeq Version	XM_017322384.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322384.1

ORF Insert Sequence:

ATGACAGATG CCCTCGTGAG CAACAAGCCT GACTTTGTCC GCCTCTTTGT GGACAGCGGT 
GCTGACATGG CCGAGTTCTT GACCTATGGG CGGCTGCAGC AGCTTTACCA TTCTGTGTCC 
CCCAAGAGCC TCCTCTTTGA ACTGCTGCAG CGTAAGCATG AGGAGGGTAG GCTGACACTG 
GCCGGCCTGG GTGCCCAGCA GGCTCGGGAG CTGCCCATTG GTCTGCCTGC CTTCTCACTC 
CACGAGGTCT CCCGCGTACT CAAAGACTTC CTGCATGACG CCTGCCGTGG CTTCTACCAG 
GACGGGCGCA GGATGGAGGA GAGAGGGCCA CCTAAGCGGC CCGCAGGCCA GAAGTGGCTG 
CCAGACCTCA GTAGGAAGAG TGAAGACCCT TGGAGGGACC TGTTCCTCTG GGCTGTGCTG 
CAGAATCGTT ATGAGATGGC CACATACTTC TGGGCCATGG GCCGGGAGGG TGTGGCTGCT 
GCTCTGGCTG CCTGCAAGAT CATAAAGGAA ATGTCCCACC TGGAGAAAGA GGCAGAGGTG 
GCCCGCACCA TGCGTGAGGC CAAGTATGAG CAGCTGGCCC TGGATCTTTT CTCAGAGTGC 
TACGGCAACA GTGAGGACCG TGCCTTTGCC CTGCTGGTGC GAAGGAACCA CAGCTGGAGC 
AGGACCACGT GCCTGCACCT GGCCACTGAA GCTGATGCCA AGGCCTTCTT TGCCCATGAC 
GGTGTGCAAG CATTCCTGAC CAAGATCTGG TGGGGAGACA TGGCCACAGG CACACCCATC 
CTACGGCTTC TGGGTGCCTT CACCTGCCCA GCCCTCATCT ACACAAACCT CATCTCCTTC 
AGTGAGGATG CCCCGCAGAG GATGGACCTA GAAGATCTGC AGGAGCCAGA CAGCTTGGAT 
ATGGAAAAGA GCTTCCTATG CAGCCGGGGT GGCCAATTGG AGAAGCTAAC AGAGGCACCA 
AGGGCTCCAG GCGATCTAGG CCCACAAGCT GCCTTCCTGC TCACACGGTG GAGGAAGTTC 
TGGGGCGCTC CTGTGACTGT GTTCCTGGGG AATGTGGTCA TGTACTTCGC ATTCCTCTTC 
CTGTTCACCT ATGTCCTGCT GGTGGACTTC AGGCCACCAC CCCAGGGGCC GTCTGGATCC 
GAGGTTACCC TCTATTTCTG GGTGTTCACA CTGGTGCTGG AGGAAATCCG ACAGGGCTTC 
TTCACAGATG AGGACACGCA CCTGGTGAAG AAATTCACTC TGTATGTGGA AGACAACTGG 
AACAAGTGTG ACATGGTGGC CATCTTCCTG TTCATTGTGG GAGTCACCTG TAGAATGGTG 
CCCTCGGTGT TTGAGGCTGG CAGGACCGTT CTGGCCATTG ACTTCATGGT GTTCACACTT 
CGGCTCATCC ACATCTTTGC TATTCACAAG CAGTTGGGTC CTAAGATCAT CATTGTAGAG 
CGAATGATGA AGGATGTCTT CTTTTTCCTC TTCTTCCTGA GCGTATGGCT TGTGGCCTAT 
GGTGTGACCA CTCAGGCCCT GCTGCATCCC CATGATGGCC GTTTGGAGTG GATTTTCCGC 
CGTGTGCTAT ACAGGCCTTA CCTGCAGATC TTTGGGCAAA TCCCTCTGGA TGAAATTGAT 
GAGGCTCGTG TGAACTGTTC TCTTCACCCT CTGCTGCTGG AAAGCTCGGC TTCCTGCCCT 
AATCTCTATG CCAACTGGCT GGTCATTCTC CTGCTGGTTA CCTTCCTGCT TGTCACTAAT 
GTGCTGCTCA TGAACCTTCT GATCGCCATG TTCAGCTACA CATTCCAGGT GGTGCAAGGC 
AATGCAGACA TGTTCTGGAA GTTTCAACGC TACCACCTCA TCGTTGAATA CCATGGAAGA 
CCAGCTCTGG CCCCGCCCTT CATCCTGCTC AGCCACCTGA GCCTGGTGCT CAAGCAGGTC 
TTCAGGAAGG AAGCCCAGCA TAAGCGACAA CATCTGGAGA GAGACTTGCC TGACCCCTTG 
GACCAGAAGA TCATTACCTG GGAAACGGTT CAAAAGGAGA ACTTCCTGAG TACCATGGAG 
AAACGGAGGA GGGACAGCGA GGGGGAGGTG CTGAGGAAAA CGGCACACAG AGTGGACTTG 
ATTGCCAAAT ACATCGGGGG GCTGAGAGAG CAAGAAAAGA GGATCAAGTG TCTGGAATCA 
CAGGCCAACT ACTGTATGCT CCTCTTGTCC TCTATGACGG ATACACTGGC TCCAGGAGGC 
ACCTACTCAA GCTCTCAGAA CTGTGGTTGC AGGAGTCAGC CAGCCTCTGC TAGAGACAGG 
GAGTACCTAG AGTCTGGCTT GCCACCCTCT GACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm5 ( XM_030242820.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098680.1 Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X4, mRNA.

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CloneID	OMu82156
Clone ID Related Accession (Same CDS sequence)	XM_017322384.1 , XM_030242820.1
Accession Version	XM_030242820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGATG CCCTCGTGAG CAACAAGCCT GACTTTGTCC GCCTCTTTGT GGACAGCGGT 
GCTGACATGG CCGAGTTCTT GACCTATGGG CGGCTGCAGC AGCTTTACCA TTCTGTGTCC 
CCCAAGAGCC TCCTCTTTGA ACTGCTGCAG CGTAAGCATG AGGAGGGTAG GCTGACACTG 
GCCGGCCTGG GTGCCCAGCA GGCTCGGGAG CTGCCCATTG GTCTGCCTGC CTTCTCACTC 
CACGAGGTCT CCCGCGTACT CAAAGACTTC CTGCATGACG CCTGCCGTGG CTTCTACCAG 
GACGGGCGCA GGATGGAGGA GAGAGGGCCA CCTAAGCGGC CCGCAGGCCA GAAGTGGCTG 
CCAGACCTCA GTAGGAAGAG TGAAGACCCT TGGAGGGACC TGTTCCTCTG GGCTGTGCTG 
CAGAATCGTT ATGAGATGGC CACATACTTC TGGGCCATGG GCCGGGAGGG TGTGGCTGCT 
GCTCTGGCTG CCTGCAAGAT CATAAAGGAA ATGTCCCACC TGGAGAAAGA GGCAGAGGTG 
GCCCGCACCA TGCGTGAGGC CAAGTATGAG CAGCTGGCCC TGGATCTTTT CTCAGAGTGC 
TACGGCAACA GTGAGGACCG TGCCTTTGCC CTGCTGGTGC GAAGGAACCA CAGCTGGAGC 
AGGACCACGT GCCTGCACCT GGCCACTGAA GCTGATGCCA AGGCCTTCTT TGCCCATGAC 
GGTGTGCAAG CATTCCTGAC CAAGATCTGG TGGGGAGACA TGGCCACAGG CACACCCATC 
CTACGGCTTC TGGGTGCCTT CACCTGCCCA GCCCTCATCT ACACAAACCT CATCTCCTTC 
AGTGAGGATG CCCCGCAGAG GATGGACCTA GAAGATCTGC AGGAGCCAGA CAGCTTGGAT 
ATGGAAAAGA GCTTCCTATG CAGCCGGGGT GGCCAATTGG AGAAGCTAAC AGAGGCACCA 
AGGGCTCCAG GCGATCTAGG CCCACAAGCT GCCTTCCTGC TCACACGGTG GAGGAAGTTC 
TGGGGCGCTC CTGTGACTGT GTTCCTGGGG AATGTGGTCA TGTACTTCGC ATTCCTCTTC 
CTGTTCACCT ATGTCCTGCT GGTGGACTTC AGGCCACCAC CCCAGGGGCC GTCTGGATCC 
GAGGTTACCC TCTATTTCTG GGTGTTCACA CTGGTGCTGG AGGAAATCCG ACAGGGCTTC 
TTCACAGATG AGGACACGCA CCTGGTGAAG AAATTCACTC TGTATGTGGA AGACAACTGG 
AACAAGTGTG ACATGGTGGC CATCTTCCTG TTCATTGTGG GAGTCACCTG TAGAATGGTG 
CCCTCGGTGT TTGAGGCTGG CAGGACCGTT CTGGCCATTG ACTTCATGGT GTTCACACTT 
CGGCTCATCC ACATCTTTGC TATTCACAAG CAGTTGGGTC CTAAGATCAT CATTGTAGAG 
CGAATGATGA AGGATGTCTT CTTTTTCCTC TTCTTCCTGA GCGTATGGCT TGTGGCCTAT 
GGTGTGACCA CTCAGGCCCT GCTGCATCCC CATGATGGCC GTTTGGAGTG GATTTTCCGC 
CGTGTGCTAT ACAGGCCTTA CCTGCAGATC TTTGGGCAAA TCCCTCTGGA TGAAATTGAT 
GAGGCTCGTG TGAACTGTTC TCTTCACCCT CTGCTGCTGG AAAGCTCGGC TTCCTGCCCT 
AATCTCTATG CCAACTGGCT GGTCATTCTC CTGCTGGTTA CCTTCCTGCT TGTCACTAAT 
GTGCTGCTCA TGAACCTTCT GATCGCCATG TTCAGCTACA CATTCCAGGT GGTGCAAGGC 
AATGCAGACA TGTTCTGGAA GTTTCAACGC TACCACCTCA TCGTTGAATA CCATGGAAGA 
CCAGCTCTGG CCCCGCCCTT CATCCTGCTC AGCCACCTGA GCCTGGTGCT CAAGCAGGTC 
TTCAGGAAGG AAGCCCAGCA TAAGCGACAA CATCTGGAGA GAGACTTGCC TGACCCCTTG 
GACCAGAAGA TCATTACCTG GGAAACGGTT CAAAAGGAGA ACTTCCTGAG TACCATGGAG 
AAACGGAGGA GGGACAGCGA GGGGGAGGTG CTGAGGAAAA CGGCACACAG AGTGGACTTG 
ATTGCCAAAT ACATCGGGGG GCTGAGAGAG CAAGAAAAGA GGATCAAGTG TCTGGAATCA 
CAGGCCAACT ACTGTATGCT CCTCTTGTCC TCTATGACGG ATACACTGGC TCCAGGAGGC 
ACCTACTCAA GCTCTCAGAA CTGTGGTTGC AGGAGTCAGC CAGCCTCTGC TAGAGACAGG 
GAGTACCTAG AGTCTGGCTT GCCACCCTCT GACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098680.1
CDS	760..3078
Translation	MTDALVSNKPDFVRLFVDSGADMAEFLTYGRLQQLYHSVSPKSLLFELLQRKHEEGRLTLAGLGAQQARELPIGLPAFSLHEVSRVLKDFLHDACRGFYQDGRRMEERGPPKRPAGQKWLPDLSRKSEDPWRDLFLWAVLQNRYEMATYFWAMGREGVAAALAACKIIKEMSHLEKEAEVARTMREAKYEQLALDLFSECYGNSEDRAFALLVRRNHSWSRTTCLHLATEADAKAFFAHDGVQAFLTKIWWGDMATGTPILRLLGAFTCPALIYTNLISFSEDAPQRMDLEDLQEPDSLDMEKSFLCSRGGQLEKLTEAPRAPGDLGPQAAFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFTYVLLVDFRPPPQGPSGSEVTLYFWVFTLVLEEIRQGFFTDEDTHLVKKFTLYVEDNWNKCDMVAIFLFIVGVTCRMVPSVFEAGRTVLAIDFMVFTLRLIHIFAIHKQLGPKIIIVERMMKDVFFFLFFLSVWLVAYGVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSLHPLLLESSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYHLIVEYHGRPALAPPFILLSHLSLVLKQVFRKEAQHKRQHLERDLPDPLDQKIITWETVQKENFLSTMEKRRRDSEGEVLRKTAHRVDLIAKYIGGLREQEKRIKCLESQANYCMLLLSSMTDTLAPGGTYSSSQNCGCRSQPASARDREYLESGLPPSDT

Target ORF information:

RefSeq Version	XM_030242820.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242820.1

ORF Insert Sequence:

ATGACAGATG CCCTCGTGAG CAACAAGCCT GACTTTGTCC GCCTCTTTGT GGACAGCGGT 
GCTGACATGG CCGAGTTCTT GACCTATGGG CGGCTGCAGC AGCTTTACCA TTCTGTGTCC 
CCCAAGAGCC TCCTCTTTGA ACTGCTGCAG CGTAAGCATG AGGAGGGTAG GCTGACACTG 
GCCGGCCTGG GTGCCCAGCA GGCTCGGGAG CTGCCCATTG GTCTGCCTGC CTTCTCACTC 
CACGAGGTCT CCCGCGTACT CAAAGACTTC CTGCATGACG CCTGCCGTGG CTTCTACCAG 
GACGGGCGCA GGATGGAGGA GAGAGGGCCA CCTAAGCGGC CCGCAGGCCA GAAGTGGCTG 
CCAGACCTCA GTAGGAAGAG TGAAGACCCT TGGAGGGACC TGTTCCTCTG GGCTGTGCTG 
CAGAATCGTT ATGAGATGGC CACATACTTC TGGGCCATGG GCCGGGAGGG TGTGGCTGCT 
GCTCTGGCTG CCTGCAAGAT CATAAAGGAA ATGTCCCACC TGGAGAAAGA GGCAGAGGTG 
GCCCGCACCA TGCGTGAGGC CAAGTATGAG CAGCTGGCCC TGGATCTTTT CTCAGAGTGC 
TACGGCAACA GTGAGGACCG TGCCTTTGCC CTGCTGGTGC GAAGGAACCA CAGCTGGAGC 
AGGACCACGT GCCTGCACCT GGCCACTGAA GCTGATGCCA AGGCCTTCTT TGCCCATGAC 
GGTGTGCAAG CATTCCTGAC CAAGATCTGG TGGGGAGACA TGGCCACAGG CACACCCATC 
CTACGGCTTC TGGGTGCCTT CACCTGCCCA GCCCTCATCT ACACAAACCT CATCTCCTTC 
AGTGAGGATG CCCCGCAGAG GATGGACCTA GAAGATCTGC AGGAGCCAGA CAGCTTGGAT 
ATGGAAAAGA GCTTCCTATG CAGCCGGGGT GGCCAATTGG AGAAGCTAAC AGAGGCACCA 
AGGGCTCCAG GCGATCTAGG CCCACAAGCT GCCTTCCTGC TCACACGGTG GAGGAAGTTC 
TGGGGCGCTC CTGTGACTGT GTTCCTGGGG AATGTGGTCA TGTACTTCGC ATTCCTCTTC 
CTGTTCACCT ATGTCCTGCT GGTGGACTTC AGGCCACCAC CCCAGGGGCC GTCTGGATCC 
GAGGTTACCC TCTATTTCTG GGTGTTCACA CTGGTGCTGG AGGAAATCCG ACAGGGCTTC 
TTCACAGATG AGGACACGCA CCTGGTGAAG AAATTCACTC TGTATGTGGA AGACAACTGG 
AACAAGTGTG ACATGGTGGC CATCTTCCTG TTCATTGTGG GAGTCACCTG TAGAATGGTG 
CCCTCGGTGT TTGAGGCTGG CAGGACCGTT CTGGCCATTG ACTTCATGGT GTTCACACTT 
CGGCTCATCC ACATCTTTGC TATTCACAAG CAGTTGGGTC CTAAGATCAT CATTGTAGAG 
CGAATGATGA AGGATGTCTT CTTTTTCCTC TTCTTCCTGA GCGTATGGCT TGTGGCCTAT 
GGTGTGACCA CTCAGGCCCT GCTGCATCCC CATGATGGCC GTTTGGAGTG GATTTTCCGC 
CGTGTGCTAT ACAGGCCTTA CCTGCAGATC TTTGGGCAAA TCCCTCTGGA TGAAATTGAT 
GAGGCTCGTG TGAACTGTTC TCTTCACCCT CTGCTGCTGG AAAGCTCGGC TTCCTGCCCT 
AATCTCTATG CCAACTGGCT GGTCATTCTC CTGCTGGTTA CCTTCCTGCT TGTCACTAAT 
GTGCTGCTCA TGAACCTTCT GATCGCCATG TTCAGCTACA CATTCCAGGT GGTGCAAGGC 
AATGCAGACA TGTTCTGGAA GTTTCAACGC TACCACCTCA TCGTTGAATA CCATGGAAGA 
CCAGCTCTGG CCCCGCCCTT CATCCTGCTC AGCCACCTGA GCCTGGTGCT CAAGCAGGTC 
TTCAGGAAGG AAGCCCAGCA TAAGCGACAA CATCTGGAGA GAGACTTGCC TGACCCCTTG 
GACCAGAAGA TCATTACCTG GGAAACGGTT CAAAAGGAGA ACTTCCTGAG TACCATGGAG 
AAACGGAGGA GGGACAGCGA GGGGGAGGTG CTGAGGAAAA CGGCACACAG AGTGGACTTG 
ATTGCCAAAT ACATCGGGGG GCTGAGAGAG CAAGAAAAGA GGATCAAGTG TCTGGAATCA 
CAGGCCAACT ACTGTATGCT CCTCTTGTCC TCTATGACGG ATACACTGGC TCCAGGAGGC 
ACCTACTCAA GCTCTCAGAA CTGTGGTTGC AGGAGTCAGC CAGCCTCTGC TAGAGACAGG 
GAGTACCTAG AGTCTGGCTT GCCACCCTCT GACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm5 ( NM_020277.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064673.2 Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), mRNA.

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CloneID	OMu18787
Clone ID Related Accession (Same CDS sequence)	XM_006508645.1 , NM_020277.2
Accession Version	NM_020277.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575302400000
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF228681.1, AB039952.1 and AK044908.1. On May 23, 2007 this sequence version replaced NM_020277.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF228681.1, AY280364.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAACAA CCCAGAGCTC CTGCCCCGGC AGCCCCCCAG ATACTGAGGA TGGCTGGGAG 
CCCATCCTAT GCAGGGGAGA GATCAACTTC GGAGGGTCTG GGAAGAAGCG AGGCAAGTTT 
GTGAAGGTGC CAAGCAGTGT GGCCCCCTCC GTGCTTTTTG AACTCCTGCT CACCGAGTGG 
CACCTGCCAG CCCCCAACCT GGTGGTGTCC CTGGTGGGTG AGGAACGACC TTTGGCTATG 
AAGTCGTGGC TTCGGGATGT CCTGCGCAAG GGGCTGGTGA AAGCAGCTCA GAGCACAGGT 
GCCTGGATCC TGACCAGTGC CCTCCACGTG GGCCTGGCCC GCCATGTTGG ACAAGCTGTA 
CGTGATCACT CTCTGGCTAG CACATCCACC AAGATCCGTG TAGTGGCCAT CGGAATGGCC 
TCTCTGGATC GAATCCTTCA CCGTCAACTT CTAGATGGTG TCCACCAAAA GGAGGATACT 
CCCATCCACT ACCCAGCAGA TGAGGGCAAC ATTCAGGGAC CCCTCTGCCC CCTGGACAGC 
AATCTCTCCC ACTTCATCCT TGTGGAGTCA GGCGCCCTTG GGAGTGGGAA CGACGGGCTG 
ACAGAGCTGC AGCTGAGCCT GGAGAAGCAC ATCTCTCAGC AGAGGACAGG TTATGGGGGC 
ACCAGCTGCA TCCAGATACC TGTCCTTTGC CTGTTGGTCA ATGGTGACCC CAACACCCTA 
GAGAGGATTT CCAGGGCAGT GGAGCAGGCT GCCCCATGGC TGATCCTGGC AGGTTCTGGT 
GGCATTGCTG ATGTACTCGC TGCCCTGGTG AGCCAGCCTC ATCTCCTGGT GCCCCAGGTG 
GCTGAGAAGC AGTTCAGAGA GAAATTCCCC AGCGAGTGTT TCTCTTGGGA AGCCATTGTA 
CACTGGACAG AGCTGTTACA GAACATTGCT GCACACCCCC ACCTGCTCAC AGTATATGAC 
TTCGAGCAGG AGGGTTCGGA GGACCTGGAC ACTGTCATCC TCAAGGCACT TGTGAAAGCC 
TGCAAGAGCC ACAGCCAAGA AGCCCAAGAC TACCTAGATG AGCTCAAGTT AGCAGTGGCC 
TGGGATCGCG TGGACATTGC CAAGAGTGAA ATCTTCAATG GGGACGTGGA ATGGAAGTCC 
TGTGACTTGG AAGAGGTGAT GACAGATGCC CTCGTGAGCA ACAAGCCTGA CTTTGTCCGC 
CTCTTTGTGG ACAGCGGTGC TGACATGGCC GAGTTCTTGA CCTATGGGCG GCTGCAGCAG 
CTTTACCATT CTGTGTCCCC CAAGAGCCTC CTCTTTGAAC TGCTGCAGCG TAAGCATGAG 
GAGGGTAGGC TGACACTGGC CGGCCTGGGT GCCCAGCAGG CTCGGGAGCT GCCCATTGGT 
CTGCCTGCCT TCTCACTCCA CGAGGTCTCC CGCGTACTCA AAGACTTCCT GCATGACGCC 
TGCCGTGGCT TCTACCAGGA CGGGCGCAGG ATGGAGGAGA GAGGGCCACC TAAGCGGCCC 
GCAGGCCAGA AGTGGCTGCC AGACCTCAGT AGGAAGAGTG AAGACCCTTG GAGGGACCTG 
TTCCTCTGGG CTGTGCTGCA GAATCGTTAT GAGATGGCCA CATACTTCTG GGCCATGGGC 
CGGGAGGGTG TGGCTGCTGC TCTGGCTGCC TGCAAGATCA TAAAGGAAAT GTCCCACCTG 
GAGAAAGAGG CAGAGGTGGC CCGCACCATG CGTGAGGCCA AGTATGAGCA GCTGGCCCTG 
GATCTTTTCT CAGAGTGCTA CGGCAACAGT GAGGACCGTG CCTTTGCCCT GCTGGTGCGA 
AGGAACCACA GCTGGAGCAG GACCACGTGC CTGCACCTGG CCACTGAAGC TGATGCCAAG 
GCCTTCTTTG CCCATGACGG TGTGCAAGCA TTCCTGACCA AGATCTGGTG GGGAGACATG 
GCCACAGGCA CACCCATCCT ACGGCTTCTG GGTGCCTTCA CCTGCCCAGC CCTCATCTAC 
ACAAACCTCA TCTCCTTCAG TGAGGATGCC CCGCAGAGGA TGGACCTAGA AGATCTGCAG 
GAGCCAGACA GCTTGGATAT GGAAAAGAGC TTCCTATGCA GCCGGGGTGG CCAATTGGAG 
AAGCTAACAG AGGCACCAAG GGCTCCAGGC GATCTAGGCC CACAAGCTGC CTTCCTGCTC 
ACACGGTGGA GGAAGTTCTG GGGCGCTCCT GTGACTGTGT TCCTGGGGAA TGTGGTCATG 
TACTTCGCAT TCCTCTTCCT GTTCACCTAT GTCCTGCTGG TGGACTTCAG GCCACCACCC 
CAGGGGCCGT CTGGATCCGA GGTTACCCTC TATTTCTGGG TGTTCACACT GGTGCTGGAG 
GAAATCCGAC AGGGCTTCTT CACAGATGAG GACACGCACC TGGTGAAGAA ATTCACTCTG 
TATGTGGAAG ACAACTGGAA CAAGTGTGAC ATGGTGGCCA TCTTCCTGTT CATTGTGGGA 
GTCACCTGTA GAATGGTGCC CTCGGTGTTT GAGGCTGGCA GGACCGTTCT GGCCATTGAC 
TTCATGGTGT TCACACTTCG GCTCATCCAC ATCTTTGCTA TTCACAAGCA GTTGGGTCCT 
AAGATCATCA TTGTAGAGCG AATGATGAAG GATGTCTTCT TTTTCCTCTT CTTCCTGAGC 
GTATGGCTTG TGGCCTATGG TGTGACCACT CAGGCCCTGC TGCATCCCCA TGATGGCCGT 
TTGGAGTGGA TTTTCCGCCG TGTGCTATAC AGGCCTTACC TGCAGATCTT TGGGCAAATC 
CCTCTGGATG AAATTGATGA GGCTCGTGTG AACTGTTCTC TTCACCCTCT GCTGCTGGAA 
AGCTCGGCTT CCTGCCCTAA TCTCTATGCC AACTGGCTGG TCATTCTCCT GCTGGTTACC 
TTCCTGCTTG TCACTAATGT GCTGCTCATG AACCTTCTGA TCGCCATGTT CAGCTACACA 
TTCCAGGTGG TGCAAGGCAA TGCAGACATG TTCTGGAAGT TTCAACGCTA CCACCTCATC 
GTTGAATACC ATGGAAGACC AGCTCTGGCC CCGCCCTTCA TCCTGCTCAG CCACCTGAGC 
CTGGTGCTCA AGCAGGTCTT CAGGAAGGAA GCCCAGCATA AGCGACAACA TCTGGAGAGA 
GACTTGCCTG ACCCCTTGGA CCAGAAGATC ATTACCTGGG AAACGGTTCA AAAGGAGAAC 
TTCCTGAGTA CCATGGAGAA ACGGAGGAGG GACAGCGAGG GGGAGGTGCT GAGGAAAACG 
GCACACAGAG TGGACTTGAT TGCCAAATAC ATCGGGGGGC TGAGAGAGCA AGAAAAGAGG 
ATCAAGTGTC TGGAATCACA GGCCAACTAC TGTATGCTCC TCTTGTCCTC TATGACGGAT 
ACACTGGCTC CAGGAGGCAC CTACTCAAGC TCTCAGAACT GTGGTTGCAG GAGTCAGCCA 
GCCTCTGCTA GAGACAGGGA GTACCTAGAG TCTGGCTTGC CACCCTCTGA CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064673.2
CDS	88..3564
Translation	MQTTQSSCPGSPPDTEDGWEPILCRGEINFGGSGKKRGKFVKVPSSVAPSVLFELLLTEWHLPAPNLVVSLVGEERPLAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKIRVVAIGMASLDRILHRQLLDGVHQKEDTPIHYPADEGNIQGPLCPLDSNLSHFILVESGALGSGNDGLTELQLSLEKHISQQRTGYGGTSCIQIPVLCLLVNGDPNTLERISRAVEQAAPWLILAGSGGIADVLAALVSQPHLLVPQVAEKQFREKFPSECFSWEAIVHWTELLQNIAAHPHLLTVYDFEQEGSEDLDTVILKALVKACKSHSQEAQDYLDELKLAVAWDRVDIAKSEIFNGDVEWKSCDLEEVMTDALVSNKPDFVRLFVDSGADMAEFLTYGRLQQLYHSVSPKSLLFELLQRKHEEGRLTLAGLGAQQARELPIGLPAFSLHEVSRVLKDFLHDACRGFYQDGRRMEERGPPKRPAGQKWLPDLSRKSEDPWRDLFLWAVLQNRYEMATYFWAMGREGVAAALAACKIIKEMSHLEKEAEVARTMREAKYEQLALDLFSECYGNSEDRAFALLVRRNHSWSRTTCLHLATEADAKAFFAHDGVQAFLTKIWWGDMATGTPILRLLGAFTCPALIYTNLISFSEDAPQRMDLEDLQEPDSLDMEKSFLCSRGGQLEKLTEAPRAPGDLGPQAAFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFTYVLLVDFRPPPQGPSGSEVTLYFWVFTLVLEEIRQGFFTDEDTHLVKKFTLYVEDNWNKCDMVAIFLFIVGVTCRMVPSVFEAGRTVLAIDFMVFTLRLIHIFAIHKQLGPKIIIVERMMKDVFFFLFFLSVWLVAYGVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSLHPLLLESSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYHLIVEYHGRPALAPPFILLSHLSLVLKQVFRKEAQHKRQHLERDLPDPLDQKIITWETVQKENFLSTMEKRRRDSEGEVLRKTAHRVDLIAKYIGGLREQEKRIKCLESQANYCMLLLSSMTDTLAPGGTYSSSQNCGCRSQPASARDREYLESGLPPSDT

Target ORF information:

RefSeq Version	NM_020277.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020277.2

ORF Insert Sequence:

ATGCAAACAA CCCAGAGCTC CTGCCCCGGC AGCCCCCCAG ATACTGAGGA TGGCTGGGAG 
CCCATCCTAT GCAGGGGAGA GATCAACTTC GGAGGGTCTG GGAAGAAGCG AGGCAAGTTT 
GTGAAGGTGC CAAGCAGTGT GGCCCCCTCC GTGCTTTTTG AACTCCTGCT CACCGAGTGG 
CACCTGCCAG CCCCCAACCT GGTGGTGTCC CTGGTGGGTG AGGAACGACC TTTGGCTATG 
AAGTCGTGGC TTCGGGATGT CCTGCGCAAG GGGCTGGTGA AAGCAGCTCA GAGCACAGGT 
GCCTGGATCC TGACCAGTGC CCTCCACGTG GGCCTGGCCC GCCATGTTGG ACAAGCTGTA 
CGTGATCACT CTCTGGCTAG CACATCCACC AAGATCCGTG TAGTGGCCAT CGGAATGGCC 
TCTCTGGATC GAATCCTTCA CCGTCAACTT CTAGATGGTG TCCACCAAAA GGAGGATACT 
CCCATCCACT ACCCAGCAGA TGAGGGCAAC ATTCAGGGAC CCCTCTGCCC CCTGGACAGC 
AATCTCTCCC ACTTCATCCT TGTGGAGTCA GGCGCCCTTG GGAGTGGGAA CGACGGGCTG 
ACAGAGCTGC AGCTGAGCCT GGAGAAGCAC ATCTCTCAGC AGAGGACAGG TTATGGGGGC 
ACCAGCTGCA TCCAGATACC TGTCCTTTGC CTGTTGGTCA ATGGTGACCC CAACACCCTA 
GAGAGGATTT CCAGGGCAGT GGAGCAGGCT GCCCCATGGC TGATCCTGGC AGGTTCTGGT 
GGCATTGCTG ATGTACTCGC TGCCCTGGTG AGCCAGCCTC ATCTCCTGGT GCCCCAGGTG 
GCTGAGAAGC AGTTCAGAGA GAAATTCCCC AGCGAGTGTT TCTCTTGGGA AGCCATTGTA 
CACTGGACAG AGCTGTTACA GAACATTGCT GCACACCCCC ACCTGCTCAC AGTATATGAC 
TTCGAGCAGG AGGGTTCGGA GGACCTGGAC ACTGTCATCC TCAAGGCACT TGTGAAAGCC 
TGCAAGAGCC ACAGCCAAGA AGCCCAAGAC TACCTAGATG AGCTCAAGTT AGCAGTGGCC 
TGGGATCGCG TGGACATTGC CAAGAGTGAA ATCTTCAATG GGGACGTGGA ATGGAAGTCC 
TGTGACTTGG AAGAGGTGAT GACAGATGCC CTCGTGAGCA ACAAGCCTGA CTTTGTCCGC 
CTCTTTGTGG ACAGCGGTGC TGACATGGCC GAGTTCTTGA CCTATGGGCG GCTGCAGCAG 
CTTTACCATT CTGTGTCCCC CAAGAGCCTC CTCTTTGAAC TGCTGCAGCG TAAGCATGAG 
GAGGGTAGGC TGACACTGGC CGGCCTGGGT GCCCAGCAGG CTCGGGAGCT GCCCATTGGT 
CTGCCTGCCT TCTCACTCCA CGAGGTCTCC CGCGTACTCA AAGACTTCCT GCATGACGCC 
TGCCGTGGCT TCTACCAGGA CGGGCGCAGG ATGGAGGAGA GAGGGCCACC TAAGCGGCCC 
GCAGGCCAGA AGTGGCTGCC AGACCTCAGT AGGAAGAGTG AAGACCCTTG GAGGGACCTG 
TTCCTCTGGG CTGTGCTGCA GAATCGTTAT GAGATGGCCA CATACTTCTG GGCCATGGGC 
CGGGAGGGTG TGGCTGCTGC TCTGGCTGCC TGCAAGATCA TAAAGGAAAT GTCCCACCTG 
GAGAAAGAGG CAGAGGTGGC CCGCACCATG CGTGAGGCCA AGTATGAGCA GCTGGCCCTG 
GATCTTTTCT CAGAGTGCTA CGGCAACAGT GAGGACCGTG CCTTTGCCCT GCTGGTGCGA 
AGGAACCACA GCTGGAGCAG GACCACGTGC CTGCACCTGG CCACTGAAGC TGATGCCAAG 
GCCTTCTTTG CCCATGACGG TGTGCAAGCA TTCCTGACCA AGATCTGGTG GGGAGACATG 
GCCACAGGCA CACCCATCCT ACGGCTTCTG GGTGCCTTCA CCTGCCCAGC CCTCATCTAC 
ACAAACCTCA TCTCCTTCAG TGAGGATGCC CCGCAGAGGA TGGACCTAGA AGATCTGCAG 
GAGCCAGACA GCTTGGATAT GGAAAAGAGC TTCCTATGCA GCCGGGGTGG CCAATTGGAG 
AAGCTAACAG AGGCACCAAG GGCTCCAGGC GATCTAGGCC CACAAGCTGC CTTCCTGCTC 
ACACGGTGGA GGAAGTTCTG GGGCGCTCCT GTGACTGTGT TCCTGGGGAA TGTGGTCATG 
TACTTCGCAT TCCTCTTCCT GTTCACCTAT GTCCTGCTGG TGGACTTCAG GCCACCACCC 
CAGGGGCCGT CTGGATCCGA GGTTACCCTC TATTTCTGGG TGTTCACACT GGTGCTGGAG 
GAAATCCGAC AGGGCTTCTT CACAGATGAG GACACGCACC TGGTGAAGAA ATTCACTCTG 
TATGTGGAAG ACAACTGGAA CAAGTGTGAC ATGGTGGCCA TCTTCCTGTT CATTGTGGGA 
GTCACCTGTA GAATGGTGCC CTCGGTGTTT GAGGCTGGCA GGACCGTTCT GGCCATTGAC 
TTCATGGTGT TCACACTTCG GCTCATCCAC ATCTTTGCTA TTCACAAGCA GTTGGGTCCT 
AAGATCATCA TTGTAGAGCG AATGATGAAG GATGTCTTCT TTTTCCTCTT CTTCCTGAGC 
GTATGGCTTG TGGCCTATGG TGTGACCACT CAGGCCCTGC TGCATCCCCA TGATGGCCGT 
TTGGAGTGGA TTTTCCGCCG TGTGCTATAC AGGCCTTACC TGCAGATCTT TGGGCAAATC 
CCTCTGGATG AAATTGATGA GGCTCGTGTG AACTGTTCTC TTCACCCTCT GCTGCTGGAA 
AGCTCGGCTT CCTGCCCTAA TCTCTATGCC AACTGGCTGG TCATTCTCCT GCTGGTTACC 
TTCCTGCTTG TCACTAATGT GCTGCTCATG AACCTTCTGA TCGCCATGTT CAGCTACACA 
TTCCAGGTGG TGCAAGGCAA TGCAGACATG TTCTGGAAGT TTCAACGCTA CCACCTCATC 
GTTGAATACC ATGGAAGACC AGCTCTGGCC CCGCCCTTCA TCCTGCTCAG CCACCTGAGC 
CTGGTGCTCA AGCAGGTCTT CAGGAAGGAA GCCCAGCATA AGCGACAACA TCTGGAGAGA 
GACTTGCCTG ACCCCTTGGA CCAGAAGATC ATTACCTGGG AAACGGTTCA AAAGGAGAAC 
TTCCTGAGTA CCATGGAGAA ACGGAGGAGG GACAGCGAGG GGGAGGTGCT GAGGAAAACG 
GCACACAGAG TGGACTTGAT TGCCAAATAC ATCGGGGGGC TGAGAGAGCA AGAAAAGAGG 
ATCAAGTGTC TGGAATCACA GGCCAACTAC TGTATGCTCC TCTTGTCCTC TATGACGGAT 
ACACTGGCTC CAGGAGGCAC CTACTCAAGC TCTCAGAACT GTGGTTGCAG GAGTCAGCCA 
GCCTCTGCTA GAGACAGGGA GTACCTAGAG TCTGGCTTGC CACCCTCTGA CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm5 ( XM_006508645.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508708.1 Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X1, mRNA.

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CloneID	OMu18787
Clone ID Related Accession (Same CDS sequence)	XM_006508645.1 , NM_020277.2
Accession Version	XM_006508645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACAA CCCAGAGCTC CTGCCCCGGC AGCCCCCCAG ATACTGAGGA TGGCTGGGAG 
CCCATCCTAT GCAGGGGAGA GATCAACTTC GGAGGGTCTG GGAAGAAGCG AGGCAAGTTT 
GTGAAGGTGC CAAGCAGTGT GGCCCCCTCC GTGCTTTTTG AACTCCTGCT CACCGAGTGG 
CACCTGCCAG CCCCCAACCT GGTGGTGTCC CTGGTGGGTG AGGAACGACC TTTGGCTATG 
AAGTCGTGGC TTCGGGATGT CCTGCGCAAG GGGCTGGTGA AAGCAGCTCA GAGCACAGGT 
GCCTGGATCC TGACCAGTGC CCTCCACGTG GGCCTGGCCC GCCATGTTGG ACAAGCTGTA 
CGTGATCACT CTCTGGCTAG CACATCCACC AAGATCCGTG TAGTGGCCAT CGGAATGGCC 
TCTCTGGATC GAATCCTTCA CCGTCAACTT CTAGATGGTG TCCACCAAAA GGAGGATACT 
CCCATCCACT ACCCAGCAGA TGAGGGCAAC ATTCAGGGAC CCCTCTGCCC CCTGGACAGC 
AATCTCTCCC ACTTCATCCT TGTGGAGTCA GGCGCCCTTG GGAGTGGGAA CGACGGGCTG 
ACAGAGCTGC AGCTGAGCCT GGAGAAGCAC ATCTCTCAGC AGAGGACAGG TTATGGGGGC 
ACCAGCTGCA TCCAGATACC TGTCCTTTGC CTGTTGGTCA ATGGTGACCC CAACACCCTA 
GAGAGGATTT CCAGGGCAGT GGAGCAGGCT GCCCCATGGC TGATCCTGGC AGGTTCTGGT 
GGCATTGCTG ATGTACTCGC TGCCCTGGTG AGCCAGCCTC ATCTCCTGGT GCCCCAGGTG 
GCTGAGAAGC AGTTCAGAGA GAAATTCCCC AGCGAGTGTT TCTCTTGGGA AGCCATTGTA 
CACTGGACAG AGCTGTTACA GAACATTGCT GCACACCCCC ACCTGCTCAC AGTATATGAC 
TTCGAGCAGG AGGGTTCGGA GGACCTGGAC ACTGTCATCC TCAAGGCACT TGTGAAAGCC 
TGCAAGAGCC ACAGCCAAGA AGCCCAAGAC TACCTAGATG AGCTCAAGTT AGCAGTGGCC 
TGGGATCGCG TGGACATTGC CAAGAGTGAA ATCTTCAATG GGGACGTGGA ATGGAAGTCC 
TGTGACTTGG AAGAGGTGAT GACAGATGCC CTCGTGAGCA ACAAGCCTGA CTTTGTCCGC 
CTCTTTGTGG ACAGCGGTGC TGACATGGCC GAGTTCTTGA CCTATGGGCG GCTGCAGCAG 
CTTTACCATT CTGTGTCCCC CAAGAGCCTC CTCTTTGAAC TGCTGCAGCG TAAGCATGAG 
GAGGGTAGGC TGACACTGGC CGGCCTGGGT GCCCAGCAGG CTCGGGAGCT GCCCATTGGT 
CTGCCTGCCT TCTCACTCCA CGAGGTCTCC CGCGTACTCA AAGACTTCCT GCATGACGCC 
TGCCGTGGCT TCTACCAGGA CGGGCGCAGG ATGGAGGAGA GAGGGCCACC TAAGCGGCCC 
GCAGGCCAGA AGTGGCTGCC AGACCTCAGT AGGAAGAGTG AAGACCCTTG GAGGGACCTG 
TTCCTCTGGG CTGTGCTGCA GAATCGTTAT GAGATGGCCA CATACTTCTG GGCCATGGGC 
CGGGAGGGTG TGGCTGCTGC TCTGGCTGCC TGCAAGATCA TAAAGGAAAT GTCCCACCTG 
GAGAAAGAGG CAGAGGTGGC CCGCACCATG CGTGAGGCCA AGTATGAGCA GCTGGCCCTG 
GATCTTTTCT CAGAGTGCTA CGGCAACAGT GAGGACCGTG CCTTTGCCCT GCTGGTGCGA 
AGGAACCACA GCTGGAGCAG GACCACGTGC CTGCACCTGG CCACTGAAGC TGATGCCAAG 
GCCTTCTTTG CCCATGACGG TGTGCAAGCA TTCCTGACCA AGATCTGGTG GGGAGACATG 
GCCACAGGCA CACCCATCCT ACGGCTTCTG GGTGCCTTCA CCTGCCCAGC CCTCATCTAC 
ACAAACCTCA TCTCCTTCAG TGAGGATGCC CCGCAGAGGA TGGACCTAGA AGATCTGCAG 
GAGCCAGACA GCTTGGATAT GGAAAAGAGC TTCCTATGCA GCCGGGGTGG CCAATTGGAG 
AAGCTAACAG AGGCACCAAG GGCTCCAGGC GATCTAGGCC CACAAGCTGC CTTCCTGCTC 
ACACGGTGGA GGAAGTTCTG GGGCGCTCCT GTGACTGTGT TCCTGGGGAA TGTGGTCATG 
TACTTCGCAT TCCTCTTCCT GTTCACCTAT GTCCTGCTGG TGGACTTCAG GCCACCACCC 
CAGGGGCCGT CTGGATCCGA GGTTACCCTC TATTTCTGGG TGTTCACACT GGTGCTGGAG 
GAAATCCGAC AGGGCTTCTT CACAGATGAG GACACGCACC TGGTGAAGAA ATTCACTCTG 
TATGTGGAAG ACAACTGGAA CAAGTGTGAC ATGGTGGCCA TCTTCCTGTT CATTGTGGGA 
GTCACCTGTA GAATGGTGCC CTCGGTGTTT GAGGCTGGCA GGACCGTTCT GGCCATTGAC 
TTCATGGTGT TCACACTTCG GCTCATCCAC ATCTTTGCTA TTCACAAGCA GTTGGGTCCT 
AAGATCATCA TTGTAGAGCG AATGATGAAG GATGTCTTCT TTTTCCTCTT CTTCCTGAGC 
GTATGGCTTG TGGCCTATGG TGTGACCACT CAGGCCCTGC TGCATCCCCA TGATGGCCGT 
TTGGAGTGGA TTTTCCGCCG TGTGCTATAC AGGCCTTACC TGCAGATCTT TGGGCAAATC 
CCTCTGGATG AAATTGATGA GGCTCGTGTG AACTGTTCTC TTCACCCTCT GCTGCTGGAA 
AGCTCGGCTT CCTGCCCTAA TCTCTATGCC AACTGGCTGG TCATTCTCCT GCTGGTTACC 
TTCCTGCTTG TCACTAATGT GCTGCTCATG AACCTTCTGA TCGCCATGTT CAGCTACACA 
TTCCAGGTGG TGCAAGGCAA TGCAGACATG TTCTGGAAGT TTCAACGCTA CCACCTCATC 
GTTGAATACC ATGGAAGACC AGCTCTGGCC CCGCCCTTCA TCCTGCTCAG CCACCTGAGC 
CTGGTGCTCA AGCAGGTCTT CAGGAAGGAA GCCCAGCATA AGCGACAACA TCTGGAGAGA 
GACTTGCCTG ACCCCTTGGA CCAGAAGATC ATTACCTGGG AAACGGTTCA AAAGGAGAAC 
TTCCTGAGTA CCATGGAGAA ACGGAGGAGG GACAGCGAGG GGGAGGTGCT GAGGAAAACG 
GCACACAGAG TGGACTTGAT TGCCAAATAC ATCGGGGGGC TGAGAGAGCA AGAAAAGAGG 
ATCAAGTGTC TGGAATCACA GGCCAACTAC TGTATGCTCC TCTTGTCCTC TATGACGGAT 
ACACTGGCTC CAGGAGGCAC CTACTCAAGC TCTCAGAACT GTGGTTGCAG GAGTCAGCCA 
GCCTCTGCTA GAGACAGGGA GTACCTAGAG TCTGGCTTGC CACCCTCTGA CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508708.1
CDS	64..3540
Translation	MQTTQSSCPGSPPDTEDGWEPILCRGEINFGGSGKKRGKFVKVPSSVAPSVLFELLLTEWHLPAPNLVVSLVGEERPLAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKIRVVAIGMASLDRILHRQLLDGVHQKEDTPIHYPADEGNIQGPLCPLDSNLSHFILVESGALGSGNDGLTELQLSLEKHISQQRTGYGGTSCIQIPVLCLLVNGDPNTLERISRAVEQAAPWLILAGSGGIADVLAALVSQPHLLVPQVAEKQFREKFPSECFSWEAIVHWTELLQNIAAHPHLLTVYDFEQEGSEDLDTVILKALVKACKSHSQEAQDYLDELKLAVAWDRVDIAKSEIFNGDVEWKSCDLEEVMTDALVSNKPDFVRLFVDSGADMAEFLTYGRLQQLYHSVSPKSLLFELLQRKHEEGRLTLAGLGAQQARELPIGLPAFSLHEVSRVLKDFLHDACRGFYQDGRRMEERGPPKRPAGQKWLPDLSRKSEDPWRDLFLWAVLQNRYEMATYFWAMGREGVAAALAACKIIKEMSHLEKEAEVARTMREAKYEQLALDLFSECYGNSEDRAFALLVRRNHSWSRTTCLHLATEADAKAFFAHDGVQAFLTKIWWGDMATGTPILRLLGAFTCPALIYTNLISFSEDAPQRMDLEDLQEPDSLDMEKSFLCSRGGQLEKLTEAPRAPGDLGPQAAFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFTYVLLVDFRPPPQGPSGSEVTLYFWVFTLVLEEIRQGFFTDEDTHLVKKFTLYVEDNWNKCDMVAIFLFIVGVTCRMVPSVFEAGRTVLAIDFMVFTLRLIHIFAIHKQLGPKIIIVERMMKDVFFFLFFLSVWLVAYGVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSLHPLLLESSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYHLIVEYHGRPALAPPFILLSHLSLVLKQVFRKEAQHKRQHLERDLPDPLDQKIITWETVQKENFLSTMEKRRRDSEGEVLRKTAHRVDLIAKYIGGLREQEKRIKCLESQANYCMLLLSSMTDTLAPGGTYSSSQNCGCRSQPASARDREYLESGLPPSDT

Target ORF information:

RefSeq Version	XM_006508645.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508645.1

ORF Insert Sequence:

ATGCAAACAA CCCAGAGCTC CTGCCCCGGC AGCCCCCCAG ATACTGAGGA TGGCTGGGAG 
CCCATCCTAT GCAGGGGAGA GATCAACTTC GGAGGGTCTG GGAAGAAGCG AGGCAAGTTT 
GTGAAGGTGC CAAGCAGTGT GGCCCCCTCC GTGCTTTTTG AACTCCTGCT CACCGAGTGG 
CACCTGCCAG CCCCCAACCT GGTGGTGTCC CTGGTGGGTG AGGAACGACC TTTGGCTATG 
AAGTCGTGGC TTCGGGATGT CCTGCGCAAG GGGCTGGTGA AAGCAGCTCA GAGCACAGGT 
GCCTGGATCC TGACCAGTGC CCTCCACGTG GGCCTGGCCC GCCATGTTGG ACAAGCTGTA 
CGTGATCACT CTCTGGCTAG CACATCCACC AAGATCCGTG TAGTGGCCAT CGGAATGGCC 
TCTCTGGATC GAATCCTTCA CCGTCAACTT CTAGATGGTG TCCACCAAAA GGAGGATACT 
CCCATCCACT ACCCAGCAGA TGAGGGCAAC ATTCAGGGAC CCCTCTGCCC CCTGGACAGC 
AATCTCTCCC ACTTCATCCT TGTGGAGTCA GGCGCCCTTG GGAGTGGGAA CGACGGGCTG 
ACAGAGCTGC AGCTGAGCCT GGAGAAGCAC ATCTCTCAGC AGAGGACAGG TTATGGGGGC 
ACCAGCTGCA TCCAGATACC TGTCCTTTGC CTGTTGGTCA ATGGTGACCC CAACACCCTA 
GAGAGGATTT CCAGGGCAGT GGAGCAGGCT GCCCCATGGC TGATCCTGGC AGGTTCTGGT 
GGCATTGCTG ATGTACTCGC TGCCCTGGTG AGCCAGCCTC ATCTCCTGGT GCCCCAGGTG 
GCTGAGAAGC AGTTCAGAGA GAAATTCCCC AGCGAGTGTT TCTCTTGGGA AGCCATTGTA 
CACTGGACAG AGCTGTTACA GAACATTGCT GCACACCCCC ACCTGCTCAC AGTATATGAC 
TTCGAGCAGG AGGGTTCGGA GGACCTGGAC ACTGTCATCC TCAAGGCACT TGTGAAAGCC 
TGCAAGAGCC ACAGCCAAGA AGCCCAAGAC TACCTAGATG AGCTCAAGTT AGCAGTGGCC 
TGGGATCGCG TGGACATTGC CAAGAGTGAA ATCTTCAATG GGGACGTGGA ATGGAAGTCC 
TGTGACTTGG AAGAGGTGAT GACAGATGCC CTCGTGAGCA ACAAGCCTGA CTTTGTCCGC 
CTCTTTGTGG ACAGCGGTGC TGACATGGCC GAGTTCTTGA CCTATGGGCG GCTGCAGCAG 
CTTTACCATT CTGTGTCCCC CAAGAGCCTC CTCTTTGAAC TGCTGCAGCG TAAGCATGAG 
GAGGGTAGGC TGACACTGGC CGGCCTGGGT GCCCAGCAGG CTCGGGAGCT GCCCATTGGT 
CTGCCTGCCT TCTCACTCCA CGAGGTCTCC CGCGTACTCA AAGACTTCCT GCATGACGCC 
TGCCGTGGCT TCTACCAGGA CGGGCGCAGG ATGGAGGAGA GAGGGCCACC TAAGCGGCCC 
GCAGGCCAGA AGTGGCTGCC AGACCTCAGT AGGAAGAGTG AAGACCCTTG GAGGGACCTG 
TTCCTCTGGG CTGTGCTGCA GAATCGTTAT GAGATGGCCA CATACTTCTG GGCCATGGGC 
CGGGAGGGTG TGGCTGCTGC TCTGGCTGCC TGCAAGATCA TAAAGGAAAT GTCCCACCTG 
GAGAAAGAGG CAGAGGTGGC CCGCACCATG CGTGAGGCCA AGTATGAGCA GCTGGCCCTG 
GATCTTTTCT CAGAGTGCTA CGGCAACAGT GAGGACCGTG CCTTTGCCCT GCTGGTGCGA 
AGGAACCACA GCTGGAGCAG GACCACGTGC CTGCACCTGG CCACTGAAGC TGATGCCAAG 
GCCTTCTTTG CCCATGACGG TGTGCAAGCA TTCCTGACCA AGATCTGGTG GGGAGACATG 
GCCACAGGCA CACCCATCCT ACGGCTTCTG GGTGCCTTCA CCTGCCCAGC CCTCATCTAC 
ACAAACCTCA TCTCCTTCAG TGAGGATGCC CCGCAGAGGA TGGACCTAGA AGATCTGCAG 
GAGCCAGACA GCTTGGATAT GGAAAAGAGC TTCCTATGCA GCCGGGGTGG CCAATTGGAG 
AAGCTAACAG AGGCACCAAG GGCTCCAGGC GATCTAGGCC CACAAGCTGC CTTCCTGCTC 
ACACGGTGGA GGAAGTTCTG GGGCGCTCCT GTGACTGTGT TCCTGGGGAA TGTGGTCATG 
TACTTCGCAT TCCTCTTCCT GTTCACCTAT GTCCTGCTGG TGGACTTCAG GCCACCACCC 
CAGGGGCCGT CTGGATCCGA GGTTACCCTC TATTTCTGGG TGTTCACACT GGTGCTGGAG 
GAAATCCGAC AGGGCTTCTT CACAGATGAG GACACGCACC TGGTGAAGAA ATTCACTCTG 
TATGTGGAAG ACAACTGGAA CAAGTGTGAC ATGGTGGCCA TCTTCCTGTT CATTGTGGGA 
GTCACCTGTA GAATGGTGCC CTCGGTGTTT GAGGCTGGCA GGACCGTTCT GGCCATTGAC 
TTCATGGTGT TCACACTTCG GCTCATCCAC ATCTTTGCTA TTCACAAGCA GTTGGGTCCT 
AAGATCATCA TTGTAGAGCG AATGATGAAG GATGTCTTCT TTTTCCTCTT CTTCCTGAGC 
GTATGGCTTG TGGCCTATGG TGTGACCACT CAGGCCCTGC TGCATCCCCA TGATGGCCGT 
TTGGAGTGGA TTTTCCGCCG TGTGCTATAC AGGCCTTACC TGCAGATCTT TGGGCAAATC 
CCTCTGGATG AAATTGATGA GGCTCGTGTG AACTGTTCTC TTCACCCTCT GCTGCTGGAA 
AGCTCGGCTT CCTGCCCTAA TCTCTATGCC AACTGGCTGG TCATTCTCCT GCTGGTTACC 
TTCCTGCTTG TCACTAATGT GCTGCTCATG AACCTTCTGA TCGCCATGTT CAGCTACACA 
TTCCAGGTGG TGCAAGGCAA TGCAGACATG TTCTGGAAGT TTCAACGCTA CCACCTCATC 
GTTGAATACC ATGGAAGACC AGCTCTGGCC CCGCCCTTCA TCCTGCTCAG CCACCTGAGC 
CTGGTGCTCA AGCAGGTCTT CAGGAAGGAA GCCCAGCATA AGCGACAACA TCTGGAGAGA 
GACTTGCCTG ACCCCTTGGA CCAGAAGATC ATTACCTGGG AAACGGTTCA AAAGGAGAAC 
TTCCTGAGTA CCATGGAGAA ACGGAGGAGG GACAGCGAGG GGGAGGTGCT GAGGAAAACG 
GCACACAGAG TGGACTTGAT TGCCAAATAC ATCGGGGGGC TGAGAGAGCA AGAAAAGAGG 
ATCAAGTGTC TGGAATCACA GGCCAACTAC TGTATGCTCC TCTTGTCCTC TATGACGGAT 
ACACTGGCTC CAGGAGGCAC CTACTCAAGC TCTCAGAACT GTGGTTGCAG GAGTCAGCCA 
GCCTCTGCTA GAGACAGGGA GTACCTAGAG TCTGGCTTGC CACCCTCTGA CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm5 ( XM_006508646.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508709.1 Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu40285
Clone ID Related Accession (Same CDS sequence)	XM_006508646.4 , XM_006508646.3
Accession Version	XM_006508646.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508646.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACAA CCCAGAGCTC CTGCCCCGGC AGCCCCCCAG ATACTGAGGA TGGCTGGGAG 
CCCATCCTAT GCAGGGGAGA GATCAACTTC GGAGGGTCTG GGAAGAAGCG AGGCAAGTTT 
GTGAAGGTGC CAAGCAGTGT GGCCCCCTCC GTGCTTTTTG AACTCCTGCT CACCGAGTGG 
CACCTGCCAG CCCCCAACCT GGTGGTGTCC CTGGTGGGTG AGGAACGACC TTTGGCTATG 
AAGTCGTGGC TTCGGGATGT CCTGCGCAAG GGGCTGGTGA AAGCAGCTCA GAGCACAGGT 
GCCTGGATCC TGACCAGTGC CCTCCACGTG GGCCTGGCCC GCCATGTTGG ACAAGCTGTA 
CGTGATCACT CTCTGGCTAG CACATCCACC AAGATCCGTG TAGTGGCCAT CGGAATGGCC 
TCTCTGGATC GAATCCTTCA CCGTCAACTT CTAGATGGTG TCCACCAAAA GGAGGATACT 
CCCATCCACT ACCCAGCAGA TGAGGGCAAC ATTCAGGGAC CCCTCTGCCC CCTGGACAGC 
AATCTCTCCC ACTTCATCCT TGTGGAGTCA GGCGCCCTTG GGAGTGGGAA CGACGGGCTG 
ACAGAGCTGC AGCTGAGCCT GGAGAAGCAC ATCTCTCAGC AGAGGACAGG TTATGGGGGC 
ACCAGCTGCA TCCAGATACC TGTCCTTTGC CTGTTGGTCA ATGGTGACCC CAACACCCTA 
GAGAGGATTT CCAGGGCAGT GGAGCAGGCT GCCCCATGGC TGATCCTGGC AGGTTCTGGT 
GGCATTGCTG ATGTACTCGC TGCCCTGGTG AGCCAGCCTC ATCTCCTGGT GCCCCAGGTG 
GCTGAGAAGC AGTTCAGAGA GAAATTCCCC AGCGAGTGTT TCTCTTGGGA AGCCATTGTA 
CACTGGACAG AGCTGTTACA GAACATTGCT GCACACCCCC ACCTGCTCAC AGTATATGAC 
TTCGAGCAGG AGGGTTCGGA GGACCTGGAC ACTGTCATCC TCAAGGCACT TGTGAAAGCC 
TGCAAGAGCC ACAGCCAAGA AGCCCAAGAC TACCTAGATG AGCTCAAGTT AGCAGTGGCC 
TGGGATCGCG TGGACATTGC CAAGAGTGAA ATCTTCAATG GGGACGTGGA ATGGAAGTCC 
TGTGACTTGG AAGAGGTGAT GACAGATGCC CTCGTGAGCA ACAAGCCTGA CTTTGTCCGC 
CTCTTTGTGG ACAGCGGTGC TGACATGGCC GAGTTCTTGA CCTATGGGCG GCTGCAGCAG 
CTTTACCATT CTGTGTCCCC CAAGAGCCTC CTCTTTGAAC TGCTGCAGCG TAAGCATGAG 
GAGGGTAGGC TGACACTGGC CGGCCTGGGT GCCCAGCAGG CTCGGGAGCT GCCCATTGGT 
CTGCCTGCCT TCTCACTCCA CGAGGTCTCC CGCGTACTCA AAGACTTCCT GCATGACGCC 
TGCCGTGGCT TCTACCAGGA CGGGCGCAGG ATGGAGGAGA GAGGGCCACC TAAGCGGCCC 
GCAGGCCAGA AGTGGCTGCC AGACCTCAGT AGGAAGAGTG AAGACCCTTG GAGGGACCTG 
TTCCTCTGGG CTGTGCTGCA GAATCGTTAT GAGATGGCCA CATACTTCTG GGCCATGGGC 
CGGGAGGGTG TGGCTGCTGC TCTGGCTGCC TGCAAGATCA TAAAGGAAAT GTCCCACCTG 
GAGAAAGAGG CAGAGGTGGC CCGCACCATG CGTGAGGCCA AGTATGAGCA GCTGGCCCTG 
GATCTTTTCT CAGAGTGCTA CGGCAACAGT GAGGACCGTG CCTTTGCCCT GCTGGTGCGA 
AGGAACCACA GCTGGAGCAG GACCACGTGC CTGCACCTGG CCACTGAAGC TGATGCCAAG 
GCCTTCTTTG CCCATGACGG TGTGCAAGCA TTCCTGACCA AGATCTGGTG GGGAGACATG 
GCCACAGGCA CACCCATCCT ACGGCTTCTG GGTGCCTTCA CCTGCCCAGC CCTCATCTAC 
ACAAACCTCA TCTCCTTCAG TGAGGATGCC CCGCAGAGGA TGGACCTAGA AGATCTGCAG 
GAGCCAGACA GCTTGGATAT GGAAAAGAGC TTCCTATGCA GCCGGGGTGG CCAATTGGAG 
AAGCTAACAG AGGCACCAAG GGCTCCAGGC GATCTAGGCC CACAAGCTGC CTTCCTGCTC 
ACACGGTGGA GGAAGTTCTG GGGCGCTCCT GTGACTGTGT TCCTGGGGAA TGTGGTCATG 
TACTTCGCAT TCCTCTTCCT GTTCACCTAT GTCCTGCTGG TGGACTTCAG GCCACCACCC 
CAGGGGCCGT CTGGATCCGA GGTTACCCTC TATTTCTGGG TGTTCACACT GGTGCTGGAG 
GAAATCCGAC AGGGCTTCTT CACAGATGAG GACACGCACC TGGTGAAGAA ATTCACTCTG 
TATGTGGAAG ACAACTGGAA CAAGTGTGAC ATGGTGGCCA TCTTCCTGTT CATTGTGGGA 
GTCACCTGTA GAATGGTGCC CTCGGTGTTT GAGGCTGGCA GGACCGTTCT GGCCATTGAC 
TTCATGGTGT TCACACTTCG GCTCATCCAC ATCTTTGCTA TTCACAAGCA GTTGGGTCCT 
AAGATCATCA TTGTAGAGCG AATGATGAAG GATGTCTTCT TTTTCCTCTT CTTCCTGAGC 
GTATGGCTTG TGGCCTATGG TGTGACCACT CAGGCCCTGC TGCATCCCCA TGATGGCCGT 
TTGGAGTGGA TTTTCCGCCG TGTGCTATAC AGGCCTTACC TGCAGATCTT TGGGCAAATC 
CCTCTGGATG AAATTGATGA GGCTCGTGTG AACTGTTCTC TTCACCCTCT GCTGCTGGAA 
AGCTCGGCTT CCTGCCCTAA TCTCTATGCC AACTGGCTGG TCATTCTCCT GCTGGTTACC 
TTCCTGCTTG TCACTAATGT GCTGCTCATG AACCTTCTGA TCGCCATGTT CAGGGTGCTG 
ACAGAGACAG GACCCATGTC CTGGTATTTT GCTGCTGTGT CCAGTGGGCT GGACCTGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508709.1
CDS	64..3066
Translation	MQTTQSSCPGSPPDTEDGWEPILCRGEINFGGSGKKRGKFVKVPSSVAPSVLFELLLTEWHLPAPNLVVSLVGEERPLAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKIRVVAIGMASLDRILHRQLLDGVHQKEDTPIHYPADEGNIQGPLCPLDSNLSHFILVESGALGSGNDGLTELQLSLEKHISQQRTGYGGTSCIQIPVLCLLVNGDPNTLERISRAVEQAAPWLILAGSGGIADVLAALVSQPHLLVPQVAEKQFREKFPSECFSWEAIVHWTELLQNIAAHPHLLTVYDFEQEGSEDLDTVILKALVKACKSHSQEAQDYLDELKLAVAWDRVDIAKSEIFNGDVEWKSCDLEEVMTDALVSNKPDFVRLFVDSGADMAEFLTYGRLQQLYHSVSPKSLLFELLQRKHEEGRLTLAGLGAQQARELPIGLPAFSLHEVSRVLKDFLHDACRGFYQDGRRMEERGPPKRPAGQKWLPDLSRKSEDPWRDLFLWAVLQNRYEMATYFWAMGREGVAAALAACKIIKEMSHLEKEAEVARTMREAKYEQLALDLFSECYGNSEDRAFALLVRRNHSWSRTTCLHLATEADAKAFFAHDGVQAFLTKIWWGDMATGTPILRLLGAFTCPALIYTNLISFSEDAPQRMDLEDLQEPDSLDMEKSFLCSRGGQLEKLTEAPRAPGDLGPQAAFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFTYVLLVDFRPPPQGPSGSEVTLYFWVFTLVLEEIRQGFFTDEDTHLVKKFTLYVEDNWNKCDMVAIFLFIVGVTCRMVPSVFEAGRTVLAIDFMVFTLRLIHIFAIHKQLGPKIIIVERMMKDVFFFLFFLSVWLVAYGVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSLHPLLLESSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFRVLTETGPMSWYFAAVSSGLDLQ

Target ORF information:

RefSeq Version	XM_006508646.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508646.4

ORF Insert Sequence:

ATGCAAACAA CCCAGAGCTC CTGCCCCGGC AGCCCCCCAG ATACTGAGGA TGGCTGGGAG 
CCCATCCTAT GCAGGGGAGA GATCAACTTC GGAGGGTCTG GGAAGAAGCG AGGCAAGTTT 
GTGAAGGTGC CAAGCAGTGT GGCCCCCTCC GTGCTTTTTG AACTCCTGCT CACCGAGTGG 
CACCTGCCAG CCCCCAACCT GGTGGTGTCC CTGGTGGGTG AGGAACGACC TTTGGCTATG 
AAGTCGTGGC TTCGGGATGT CCTGCGCAAG GGGCTGGTGA AAGCAGCTCA GAGCACAGGT 
GCCTGGATCC TGACCAGTGC CCTCCACGTG GGCCTGGCCC GCCATGTTGG ACAAGCTGTA 
CGTGATCACT CTCTGGCTAG CACATCCACC AAGATCCGTG TAGTGGCCAT CGGAATGGCC 
TCTCTGGATC GAATCCTTCA CCGTCAACTT CTAGATGGTG TCCACCAAAA GGAGGATACT 
CCCATCCACT ACCCAGCAGA TGAGGGCAAC ATTCAGGGAC CCCTCTGCCC CCTGGACAGC 
AATCTCTCCC ACTTCATCCT TGTGGAGTCA GGCGCCCTTG GGAGTGGGAA CGACGGGCTG 
ACAGAGCTGC AGCTGAGCCT GGAGAAGCAC ATCTCTCAGC AGAGGACAGG TTATGGGGGC 
ACCAGCTGCA TCCAGATACC TGTCCTTTGC CTGTTGGTCA ATGGTGACCC CAACACCCTA 
GAGAGGATTT CCAGGGCAGT GGAGCAGGCT GCCCCATGGC TGATCCTGGC AGGTTCTGGT 
GGCATTGCTG ATGTACTCGC TGCCCTGGTG AGCCAGCCTC ATCTCCTGGT GCCCCAGGTG 
GCTGAGAAGC AGTTCAGAGA GAAATTCCCC AGCGAGTGTT TCTCTTGGGA AGCCATTGTA 
CACTGGACAG AGCTGTTACA GAACATTGCT GCACACCCCC ACCTGCTCAC AGTATATGAC 
TTCGAGCAGG AGGGTTCGGA GGACCTGGAC ACTGTCATCC TCAAGGCACT TGTGAAAGCC 
TGCAAGAGCC ACAGCCAAGA AGCCCAAGAC TACCTAGATG AGCTCAAGTT AGCAGTGGCC 
TGGGATCGCG TGGACATTGC CAAGAGTGAA ATCTTCAATG GGGACGTGGA ATGGAAGTCC 
TGTGACTTGG AAGAGGTGAT GACAGATGCC CTCGTGAGCA ACAAGCCTGA CTTTGTCCGC 
CTCTTTGTGG ACAGCGGTGC TGACATGGCC GAGTTCTTGA CCTATGGGCG GCTGCAGCAG 
CTTTACCATT CTGTGTCCCC CAAGAGCCTC CTCTTTGAAC TGCTGCAGCG TAAGCATGAG 
GAGGGTAGGC TGACACTGGC CGGCCTGGGT GCCCAGCAGG CTCGGGAGCT GCCCATTGGT 
CTGCCTGCCT TCTCACTCCA CGAGGTCTCC CGCGTACTCA AAGACTTCCT GCATGACGCC 
TGCCGTGGCT TCTACCAGGA CGGGCGCAGG ATGGAGGAGA GAGGGCCACC TAAGCGGCCC 
GCAGGCCAGA AGTGGCTGCC AGACCTCAGT AGGAAGAGTG AAGACCCTTG GAGGGACCTG 
TTCCTCTGGG CTGTGCTGCA GAATCGTTAT GAGATGGCCA CATACTTCTG GGCCATGGGC 
CGGGAGGGTG TGGCTGCTGC TCTGGCTGCC TGCAAGATCA TAAAGGAAAT GTCCCACCTG 
GAGAAAGAGG CAGAGGTGGC CCGCACCATG CGTGAGGCCA AGTATGAGCA GCTGGCCCTG 
GATCTTTTCT CAGAGTGCTA CGGCAACAGT GAGGACCGTG CCTTTGCCCT GCTGGTGCGA 
AGGAACCACA GCTGGAGCAG GACCACGTGC CTGCACCTGG CCACTGAAGC TGATGCCAAG 
GCCTTCTTTG CCCATGACGG TGTGCAAGCA TTCCTGACCA AGATCTGGTG GGGAGACATG 
GCCACAGGCA CACCCATCCT ACGGCTTCTG GGTGCCTTCA CCTGCCCAGC CCTCATCTAC 
ACAAACCTCA TCTCCTTCAG TGAGGATGCC CCGCAGAGGA TGGACCTAGA AGATCTGCAG 
GAGCCAGACA GCTTGGATAT GGAAAAGAGC TTCCTATGCA GCCGGGGTGG CCAATTGGAG 
AAGCTAACAG AGGCACCAAG GGCTCCAGGC GATCTAGGCC CACAAGCTGC CTTCCTGCTC 
ACACGGTGGA GGAAGTTCTG GGGCGCTCCT GTGACTGTGT TCCTGGGGAA TGTGGTCATG 
TACTTCGCAT TCCTCTTCCT GTTCACCTAT GTCCTGCTGG TGGACTTCAG GCCACCACCC 
CAGGGGCCGT CTGGATCCGA GGTTACCCTC TATTTCTGGG TGTTCACACT GGTGCTGGAG 
GAAATCCGAC AGGGCTTCTT CACAGATGAG GACACGCACC TGGTGAAGAA ATTCACTCTG 
TATGTGGAAG ACAACTGGAA CAAGTGTGAC ATGGTGGCCA TCTTCCTGTT CATTGTGGGA 
GTCACCTGTA GAATGGTGCC CTCGGTGTTT GAGGCTGGCA GGACCGTTCT GGCCATTGAC 
TTCATGGTGT TCACACTTCG GCTCATCCAC ATCTTTGCTA TTCACAAGCA GTTGGGTCCT 
AAGATCATCA TTGTAGAGCG AATGATGAAG GATGTCTTCT TTTTCCTCTT CTTCCTGAGC 
GTATGGCTTG TGGCCTATGG TGTGACCACT CAGGCCCTGC TGCATCCCCA TGATGGCCGT 
TTGGAGTGGA TTTTCCGCCG TGTGCTATAC AGGCCTTACC TGCAGATCTT TGGGCAAATC 
CCTCTGGATG AAATTGATGA GGCTCGTGTG AACTGTTCTC TTCACCCTCT GCTGCTGGAA 
AGCTCGGCTT CCTGCCCTAA TCTCTATGCC AACTGGCTGG TCATTCTCCT GCTGGTTACC 
TTCCTGCTTG TCACTAATGT GCTGCTCATG AACCTTCTGA TCGCCATGTT CAGGGTGCTG 
ACAGAGACAG GACCCATGTC CTGGTATTTT GCTGCTGTGT CCAGTGGGCT GGACCTGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm5 ( XM_006508646.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508709.1 Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X2, mRNA.

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CloneID	OMu40285
Clone ID Related Accession (Same CDS sequence)	XM_006508646.4 , XM_006508646.3
Accession Version	XM_006508646.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	transient receptor potential cation channel subfamily M member 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508646.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACAA CCCAGAGCTC CTGCCCCGGC AGCCCCCCAG ATACTGAGGA TGGCTGGGAG 
CCCATCCTAT GCAGGGGAGA GATCAACTTC GGAGGGTCTG GGAAGAAGCG AGGCAAGTTT 
GTGAAGGTGC CAAGCAGTGT GGCCCCCTCC GTGCTTTTTG AACTCCTGCT CACCGAGTGG 
CACCTGCCAG CCCCCAACCT GGTGGTGTCC CTGGTGGGTG AGGAACGACC TTTGGCTATG 
AAGTCGTGGC TTCGGGATGT CCTGCGCAAG GGGCTGGTGA AAGCAGCTCA GAGCACAGGT 
GCCTGGATCC TGACCAGTGC CCTCCACGTG GGCCTGGCCC GCCATGTTGG ACAAGCTGTA 
CGTGATCACT CTCTGGCTAG CACATCCACC AAGATCCGTG TAGTGGCCAT CGGAATGGCC 
TCTCTGGATC GAATCCTTCA CCGTCAACTT CTAGATGGTG TCCACCAAAA GGAGGATACT 
CCCATCCACT ACCCAGCAGA TGAGGGCAAC ATTCAGGGAC CCCTCTGCCC CCTGGACAGC 
AATCTCTCCC ACTTCATCCT TGTGGAGTCA GGCGCCCTTG GGAGTGGGAA CGACGGGCTG 
ACAGAGCTGC AGCTGAGCCT GGAGAAGCAC ATCTCTCAGC AGAGGACAGG TTATGGGGGC 
ACCAGCTGCA TCCAGATACC TGTCCTTTGC CTGTTGGTCA ATGGTGACCC CAACACCCTA 
GAGAGGATTT CCAGGGCAGT GGAGCAGGCT GCCCCATGGC TGATCCTGGC AGGTTCTGGT 
GGCATTGCTG ATGTACTCGC TGCCCTGGTG AGCCAGCCTC ATCTCCTGGT GCCCCAGGTG 
GCTGAGAAGC AGTTCAGAGA GAAATTCCCC AGCGAGTGTT TCTCTTGGGA AGCCATTGTA 
CACTGGACAG AGCTGTTACA GAACATTGCT GCACACCCCC ACCTGCTCAC AGTATATGAC 
TTCGAGCAGG AGGGTTCGGA GGACCTGGAC ACTGTCATCC TCAAGGCACT TGTGAAAGCC 
TGCAAGAGCC ACAGCCAAGA AGCCCAAGAC TACCTAGATG AGCTCAAGTT AGCAGTGGCC 
TGGGATCGCG TGGACATTGC CAAGAGTGAA ATCTTCAATG GGGACGTGGA ATGGAAGTCC 
TGTGACTTGG AAGAGGTGAT GACAGATGCC CTCGTGAGCA ACAAGCCTGA CTTTGTCCGC 
CTCTTTGTGG ACAGCGGTGC TGACATGGCC GAGTTCTTGA CCTATGGGCG GCTGCAGCAG 
CTTTACCATT CTGTGTCCCC CAAGAGCCTC CTCTTTGAAC TGCTGCAGCG TAAGCATGAG 
GAGGGTAGGC TGACACTGGC CGGCCTGGGT GCCCAGCAGG CTCGGGAGCT GCCCATTGGT 
CTGCCTGCCT TCTCACTCCA CGAGGTCTCC CGCGTACTCA AAGACTTCCT GCATGACGCC 
TGCCGTGGCT TCTACCAGGA CGGGCGCAGG ATGGAGGAGA GAGGGCCACC TAAGCGGCCC 
GCAGGCCAGA AGTGGCTGCC AGACCTCAGT AGGAAGAGTG AAGACCCTTG GAGGGACCTG 
TTCCTCTGGG CTGTGCTGCA GAATCGTTAT GAGATGGCCA CATACTTCTG GGCCATGGGC 
CGGGAGGGTG TGGCTGCTGC TCTGGCTGCC TGCAAGATCA TAAAGGAAAT GTCCCACCTG 
GAGAAAGAGG CAGAGGTGGC CCGCACCATG CGTGAGGCCA AGTATGAGCA GCTGGCCCTG 
GATCTTTTCT CAGAGTGCTA CGGCAACAGT GAGGACCGTG CCTTTGCCCT GCTGGTGCGA 
AGGAACCACA GCTGGAGCAG GACCACGTGC CTGCACCTGG CCACTGAAGC TGATGCCAAG 
GCCTTCTTTG CCCATGACGG TGTGCAAGCA TTCCTGACCA AGATCTGGTG GGGAGACATG 
GCCACAGGCA CACCCATCCT ACGGCTTCTG GGTGCCTTCA CCTGCCCAGC CCTCATCTAC 
ACAAACCTCA TCTCCTTCAG TGAGGATGCC CCGCAGAGGA TGGACCTAGA AGATCTGCAG 
GAGCCAGACA GCTTGGATAT GGAAAAGAGC TTCCTATGCA GCCGGGGTGG CCAATTGGAG 
AAGCTAACAG AGGCACCAAG GGCTCCAGGC GATCTAGGCC CACAAGCTGC CTTCCTGCTC 
ACACGGTGGA GGAAGTTCTG GGGCGCTCCT GTGACTGTGT TCCTGGGGAA TGTGGTCATG 
TACTTCGCAT TCCTCTTCCT GTTCACCTAT GTCCTGCTGG TGGACTTCAG GCCACCACCC 
CAGGGGCCGT CTGGATCCGA GGTTACCCTC TATTTCTGGG TGTTCACACT GGTGCTGGAG 
GAAATCCGAC AGGGCTTCTT CACAGATGAG GACACGCACC TGGTGAAGAA ATTCACTCTG 
TATGTGGAAG ACAACTGGAA CAAGTGTGAC ATGGTGGCCA TCTTCCTGTT CATTGTGGGA 
GTCACCTGTA GAATGGTGCC CTCGGTGTTT GAGGCTGGCA GGACCGTTCT GGCCATTGAC 
TTCATGGTGT TCACACTTCG GCTCATCCAC ATCTTTGCTA TTCACAAGCA GTTGGGTCCT 
AAGATCATCA TTGTAGAGCG AATGATGAAG GATGTCTTCT TTTTCCTCTT CTTCCTGAGC 
GTATGGCTTG TGGCCTATGG TGTGACCACT CAGGCCCTGC TGCATCCCCA TGATGGCCGT 
TTGGAGTGGA TTTTCCGCCG TGTGCTATAC AGGCCTTACC TGCAGATCTT TGGGCAAATC 
CCTCTGGATG AAATTGATGA GGCTCGTGTG AACTGTTCTC TTCACCCTCT GCTGCTGGAA 
AGCTCGGCTT CCTGCCCTAA TCTCTATGCC AACTGGCTGG TCATTCTCCT GCTGGTTACC 
TTCCTGCTTG TCACTAATGT GCTGCTCATG AACCTTCTGA TCGCCATGTT CAGGGTGCTG 
ACAGAGACAG GACCCATGTC CTGGTATTTT GCTGCTGTGT CCAGTGGGCT GGACCTGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508709.1
CDS	64..3066
Translation	MQTTQSSCPGSPPDTEDGWEPILCRGEINFGGSGKKRGKFVKVPSSVAPSVLFELLLTEWHLPAPNLVVSLVGEERPLAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKIRVVAIGMASLDRILHRQLLDGVHQKEDTPIHYPADEGNIQGPLCPLDSNLSHFILVESGALGSGNDGLTELQLSLEKHISQQRTGYGGTSCIQIPVLCLLVNGDPNTLERISRAVEQAAPWLILAGSGGIADVLAALVSQPHLLVPQVAEKQFREKFPSECFSWEAIVHWTELLQNIAAHPHLLTVYDFEQEGSEDLDTVILKALVKACKSHSQEAQDYLDELKLAVAWDRVDIAKSEIFNGDVEWKSCDLEEVMTDALVSNKPDFVRLFVDSGADMAEFLTYGRLQQLYHSVSPKSLLFELLQRKHEEGRLTLAGLGAQQARELPIGLPAFSLHEVSRVLKDFLHDACRGFYQDGRRMEERGPPKRPAGQKWLPDLSRKSEDPWRDLFLWAVLQNRYEMATYFWAMGREGVAAALAACKIIKEMSHLEKEAEVARTMREAKYEQLALDLFSECYGNSEDRAFALLVRRNHSWSRTTCLHLATEADAKAFFAHDGVQAFLTKIWWGDMATGTPILRLLGAFTCPALIYTNLISFSEDAPQRMDLEDLQEPDSLDMEKSFLCSRGGQLEKLTEAPRAPGDLGPQAAFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFTYVLLVDFRPPPQGPSGSEVTLYFWVFTLVLEEIRQGFFTDEDTHLVKKFTLYVEDNWNKCDMVAIFLFIVGVTCRMVPSVFEAGRTVLAIDFMVFTLRLIHIFAIHKQLGPKIIIVERMMKDVFFFLFFLSVWLVAYGVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSLHPLLLESSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFRVLTETGPMSWYFAAVSSGLDLQ

Target ORF information:

RefSeq Version	XM_006508646.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508646.3

ORF Insert Sequence:

ATGCAAACAA CCCAGAGCTC CTGCCCCGGC AGCCCCCCAG ATACTGAGGA TGGCTGGGAG 
CCCATCCTAT GCAGGGGAGA GATCAACTTC GGAGGGTCTG GGAAGAAGCG AGGCAAGTTT 
GTGAAGGTGC CAAGCAGTGT GGCCCCCTCC GTGCTTTTTG AACTCCTGCT CACCGAGTGG 
CACCTGCCAG CCCCCAACCT GGTGGTGTCC CTGGTGGGTG AGGAACGACC TTTGGCTATG 
AAGTCGTGGC TTCGGGATGT CCTGCGCAAG GGGCTGGTGA AAGCAGCTCA GAGCACAGGT 
GCCTGGATCC TGACCAGTGC CCTCCACGTG GGCCTGGCCC GCCATGTTGG ACAAGCTGTA 
CGTGATCACT CTCTGGCTAG CACATCCACC AAGATCCGTG TAGTGGCCAT CGGAATGGCC 
TCTCTGGATC GAATCCTTCA CCGTCAACTT CTAGATGGTG TCCACCAAAA GGAGGATACT 
CCCATCCACT ACCCAGCAGA TGAGGGCAAC ATTCAGGGAC CCCTCTGCCC CCTGGACAGC 
AATCTCTCCC ACTTCATCCT TGTGGAGTCA GGCGCCCTTG GGAGTGGGAA CGACGGGCTG 
ACAGAGCTGC AGCTGAGCCT GGAGAAGCAC ATCTCTCAGC AGAGGACAGG TTATGGGGGC 
ACCAGCTGCA TCCAGATACC TGTCCTTTGC CTGTTGGTCA ATGGTGACCC CAACACCCTA 
GAGAGGATTT CCAGGGCAGT GGAGCAGGCT GCCCCATGGC TGATCCTGGC AGGTTCTGGT 
GGCATTGCTG ATGTACTCGC TGCCCTGGTG AGCCAGCCTC ATCTCCTGGT GCCCCAGGTG 
GCTGAGAAGC AGTTCAGAGA GAAATTCCCC AGCGAGTGTT TCTCTTGGGA AGCCATTGTA 
CACTGGACAG AGCTGTTACA GAACATTGCT GCACACCCCC ACCTGCTCAC AGTATATGAC 
TTCGAGCAGG AGGGTTCGGA GGACCTGGAC ACTGTCATCC TCAAGGCACT TGTGAAAGCC 
TGCAAGAGCC ACAGCCAAGA AGCCCAAGAC TACCTAGATG AGCTCAAGTT AGCAGTGGCC 
TGGGATCGCG TGGACATTGC CAAGAGTGAA ATCTTCAATG GGGACGTGGA ATGGAAGTCC 
TGTGACTTGG AAGAGGTGAT GACAGATGCC CTCGTGAGCA ACAAGCCTGA CTTTGTCCGC 
CTCTTTGTGG ACAGCGGTGC TGACATGGCC GAGTTCTTGA CCTATGGGCG GCTGCAGCAG 
CTTTACCATT CTGTGTCCCC CAAGAGCCTC CTCTTTGAAC TGCTGCAGCG TAAGCATGAG 
GAGGGTAGGC TGACACTGGC CGGCCTGGGT GCCCAGCAGG CTCGGGAGCT GCCCATTGGT 
CTGCCTGCCT TCTCACTCCA CGAGGTCTCC CGCGTACTCA AAGACTTCCT GCATGACGCC 
TGCCGTGGCT TCTACCAGGA CGGGCGCAGG ATGGAGGAGA GAGGGCCACC TAAGCGGCCC 
GCAGGCCAGA AGTGGCTGCC AGACCTCAGT AGGAAGAGTG AAGACCCTTG GAGGGACCTG 
TTCCTCTGGG CTGTGCTGCA GAATCGTTAT GAGATGGCCA CATACTTCTG GGCCATGGGC 
CGGGAGGGTG TGGCTGCTGC TCTGGCTGCC TGCAAGATCA TAAAGGAAAT GTCCCACCTG 
GAGAAAGAGG CAGAGGTGGC CCGCACCATG CGTGAGGCCA AGTATGAGCA GCTGGCCCTG 
GATCTTTTCT CAGAGTGCTA CGGCAACAGT GAGGACCGTG CCTTTGCCCT GCTGGTGCGA 
AGGAACCACA GCTGGAGCAG GACCACGTGC CTGCACCTGG CCACTGAAGC TGATGCCAAG 
GCCTTCTTTG CCCATGACGG TGTGCAAGCA TTCCTGACCA AGATCTGGTG GGGAGACATG 
GCCACAGGCA CACCCATCCT ACGGCTTCTG GGTGCCTTCA CCTGCCCAGC CCTCATCTAC 
ACAAACCTCA TCTCCTTCAG TGAGGATGCC CCGCAGAGGA TGGACCTAGA AGATCTGCAG 
GAGCCAGACA GCTTGGATAT GGAAAAGAGC TTCCTATGCA GCCGGGGTGG CCAATTGGAG 
AAGCTAACAG AGGCACCAAG GGCTCCAGGC GATCTAGGCC CACAAGCTGC CTTCCTGCTC 
ACACGGTGGA GGAAGTTCTG GGGCGCTCCT GTGACTGTGT TCCTGGGGAA TGTGGTCATG 
TACTTCGCAT TCCTCTTCCT GTTCACCTAT GTCCTGCTGG TGGACTTCAG GCCACCACCC 
CAGGGGCCGT CTGGATCCGA GGTTACCCTC TATTTCTGGG TGTTCACACT GGTGCTGGAG 
GAAATCCGAC AGGGCTTCTT CACAGATGAG GACACGCACC TGGTGAAGAA ATTCACTCTG 
TATGTGGAAG ACAACTGGAA CAAGTGTGAC ATGGTGGCCA TCTTCCTGTT CATTGTGGGA 
GTCACCTGTA GAATGGTGCC CTCGGTGTTT GAGGCTGGCA GGACCGTTCT GGCCATTGAC 
TTCATGGTGT TCACACTTCG GCTCATCCAC ATCTTTGCTA TTCACAAGCA GTTGGGTCCT 
AAGATCATCA TTGTAGAGCG AATGATGAAG GATGTCTTCT TTTTCCTCTT CTTCCTGAGC 
GTATGGCTTG TGGCCTATGG TGTGACCACT CAGGCCCTGC TGCATCCCCA TGATGGCCGT 
TTGGAGTGGA TTTTCCGCCG TGTGCTATAC AGGCCTTACC TGCAGATCTT TGGGCAAATC 
CCTCTGGATG AAATTGATGA GGCTCGTGTG AACTGTTCTC TTCACCCTCT GCTGCTGGAA 
AGCTCGGCTT CCTGCCCTAA TCTCTATGCC AACTGGCTGG TCATTCTCCT GCTGGTTACC 
TTCCTGCTTG TCACTAATGT GCTGCTCATG AACCTTCTGA TCGCCATGTT CAGGGTGCTG 
ACAGAGACAG GACCCATGTC CTGGTATTTT GCTGCTGTGT CCAGTGGGCT GGACCTGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impairment of Bitter Taste Sensor Transient Receptor Potential Channel M5-Mediated Aversion Aggravates High-Salt Intake and Hypertension.
Hypertension (Dallas, Tex. : 1979)74(4)1021-1032(2019 10)
Cui Y,Wu H,Li Q,Liao J,Gao P,Sun F,Zhang H,Lu Z,Wei X,He C,Ma T,Wei X,Chen X,Zheng H,Yang G,Liu D,Zhu Z

A Metabolite-Triggered Tuft Cell-ILC2 Circuit Drives Small Intestinal Remodeling.
Cell174(2)271-284.e14(2018 07)
Schneider C,O'Leary CE,von Moltke J,Liang HE,Ang QY,Turnbaugh PJ,Radhakrishnan S,Pellizzon M,Ma A,Locksley RM

Detection of Succinate by Intestinal Tuft Cells Triggers a Type 2 Innate Immune Circuit.
Immunity49(1)33-41.e7(2018 07)
Nadjsombati MS,McGinty JW,Lyons-Cohen MR,Jaffe JB,DiPeso L,Schneider C,Miller CN,Pollack JL,Nagana Gowda GA,Fontana MF,Erle DJ,Anderson MS,Locksley RM,Raftery D,von Moltke J

Thymic tuft cells promote an IL-4-enriched medulla and shape thymocyte development.
Nature559(7715)627-631(2018 07)
Miller CN,Proekt I,von Moltke J,Wells KL,Rajpurkar AR,Wang H,Rattay K,Khan IS,Metzger TC,Pollack JL,Fries AC,Lwin WW,Wigton EJ,Parent AV,Kyewski B,Erle DJ,Hogquist KA,Steinmetz LM,Locksley RM,Anderson MS

Chemical synapses without synaptic vesicles: Purinergic neurotransmission through a CALHM1 channel-mitochondrial signaling complex.
Science signaling11(529)(2018 05)
Romanov RA,Lasher RS,High B,Savidge LE,Lawson A,Rogachevskaja OA,Zhao H,Rogachevsky VV,Bystrova MF,Churbanov GD,Adameyko I,Harkany T,Yang R,Kidd GJ,Marambaud P,Kinnamon JC,Kolesnikov SS,Finger TE

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Trpm5 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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