trpm5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	trpm5
Entrez Gene ID	557722
Full Name	transient receptor potential cation channel, subfamily M, member 5
Synonyms	si:dkey-56p7.1
General protein information	Preferred Names transient receptor potential cation channel, subfamily M, member 5 Names transient receptor potential cation channel subfamily M member 5 transient receptor potential melastatin 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001128239.1	NP_001121711.1	transient receptor potential cation channel subfamily M member 5
XM_017356874.1	XP_017212363.1	transient receptor potential cation channel subfamily M member 5 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Trpm5	NP_064673.2
Pan troglodytes (chimpanzee)	TRPM5	XP_521720.4
Rattus norvegicus (Norway rat)	Trpm5	NP_001178825.1
Homo sapiens (human)	TRPM5	NP_055370.1
Macaca mulatta (Rhesus monkey)	TRPM5	XP_001093334.1
Gallus gallus (chicken)	TRPM5	XP_003641369.2
Bos taurus (cattle)	TRPM5	XP_002699492.2
Canis lupus familiaris (dog)	TRPM5	XP_005631408.1
Danio rerio (zebrafish)	trpm5	NP_001121711.1

Gene Ontology
Bibliography

Related articles in PubMed

Thermal stress in Danio rerio: a link between temperature, light, thermo-TRP channels, and clock genes.
Jerônimo R, Moraes MN, de Assis LVM, Ramos BC, Rocha T, Castrucci AML
Journal of thermal biology68(Pt A)128-138(2017 Aug)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Phylogenetic analysis and expression of zebrafish transient receptor potential melastatin family genes.
Kastenhuber E, Gesemann M, Mickoleit M, Neuhauss SC
Developmental dynamics : an official publication of the American Association of Anatomists242(11)1236-49(2013 Nov)

Epidermal keratinocyte polarity and motility require Ca²⁺ influx through TRPV1.
Graham DM, Huang L, Robinson KR, Messerli MA
Journal of cell science126(Pt 20)4602-13(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following trpm5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trpm5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36884	NM_001128239.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 5 (trpm5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa59635	XM_017356874.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 5 (trpm5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trpm5 ( NM_001128239.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001121711.1 Danio rerio transient receptor potential cation channel, subfamily M, member 5 (trpm5), mRNA.

Price & Availability↑

CloneID	ODa36884
Clone ID Related Accession (Same CDS sequence)	NM_001128239.1
Accession Version	NM_001128239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL929208.9. On Jul 31, 2008 this sequence version replaced XM_680835.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01765705 [ECO:0000350] ##Evidence-Data-END##

ATGGTCGAGA AGTCCAGTGA GAGATTTGAT AAACAGATGG CCGGGCGATT GGGAGATATT 
GATTTTACTG GTGTCAGTCG AACCAGGGGC AAGTTCGTAA GGGTGACCAG CTCCACGGAT 
CCAGCTGAGA TCTATCAGAT TTTGACTAAG CAGTGGGGTC TGGCTCCTCC TCATCTGGTG 
GTGGCACTGA TGGGAGGTGA TGAAGTTGCT CAGCTTAAGC CCTGGCTGAG GGACACACTC 
AGGAAAGGCC TCGTGAAGGC AGCACAGAGC ACAGGGGCCT GGATCCTCAC CAGTGGTTTG 
CGCTTTGGCA TCACTAAGAA TCTGGGTCAG GCCGTAAGAG ACCACTCTCT AGCGAGCACT 
TCCCCAAAAG TGCGAGTTGT GGCTATTGGA ATTGCCCCCT GGAACATGAT TCAGAACCGA 
GATCTGCTGC TGTCTGCAAA GCCTGATCAT CCAGCAACAT ACCCAACCGA GGATCTCCCA 
TATGGCGCTG TGTACTCCTT GGACTGCAAT CATTCACATT TCATCCTGGT GGACGAGGAT 
CCCAAGAGAC CTGGAGCCAC TGGAGAGATG AGGGTCAAGA TGCTCAAACA CATCTCTCTG 
CAGCGAACGG GATATGGGGG CACTGGCAGT ATAGAAATAC CTGTTTTGTG TCTTCTGGTT 
CATGGAGAGC CAAGGATATT ACAGAAAATG TACAAGAATA TTCAGAATTC AATTCCCTGG 
CTAATACTGG CTGGCTCAGG AGGAGTAGCC GATATTTTAG TGACCCTGAT GGACAGAGGA 
TGCTGGGATG CTGATATTGT TCAAGAGCTC TTGATTAACA CCTTTCCTGA TGGTCTGCAC 
AGCACTGAAA TCACTTCTTG GACCAAACTG ATTCAGAGAA TATTGGACCA TGGTCACCTG 
TTAACAGTGC ATGACCCAGA GCAGGATTCA GAACTGGATA CAGTAATTCT CAAAGCTCTT 
GTTAAGGCGT GTAAGAGTCA GAGTCAGGAA GCGCAGGACT TCCTGGATGA GCTGAAGCTG 
GCCGTGGCCT GGAATAGAGT GGACATCGCC AAAAGTGAGA TCTTCAGTGG AGACGTCCAA 
TGGAGTGCAC AGGACTTGGA GGAGGTCATG ATGGAGGCAC TGGTGAATGA CAAGCCTGAC 
TTTGTGCGAC TTTTTGTTGA TAACGGTGTA AACATAAAGC AATTCCTGAC TTATGGCCGT 
CTCCAAGAGC TCTACTGCTC TGTGTCTGAG AAAAACCTGC TTCACACTCT GCTCCTGAAA 
AAGAATCAGG AGAGACAGGC TCAGTTGGCC AGAAAACGAA TGTCAGGAAA CCCCAACAAT 
GAACTAGGTG ACCGCAAATT TCGTTTCACC TTCCATGAGG TCTCAAAAGT CCTCAAAGAC 
TTTCTTGATG ACACCTGTAA GGGTTTCTAC CAAAAACTTC CAGCGGTGAG TAAAGCTTTA 
CACCAGAAAA ACCTTCCAGA CATGGACAGA CGATGTGAGC ACCCTTGGAG AGACCTCTTC 
CTTTGGGCTA TTCTGCAGAA TAGGCAGGAA ATGGCAAACT ACTTCTGGGC CATGGGCCCG 
GAGGCAGTGG CAGCTGCTCT GGTGGGCTGT AAGATCATGA AGGAGATGGC CCATCTGGCT 
ACTGAAGCGG AGTCTGCTCG CAGTATGAAG AATGCCAAGT ATGAGCAGTT TGCAATGGAT 
CTTTTCAGTG AGTGTTATTC AAACAGTGAA GACAGAGCCT ATTCTCTTCT GGTGAGGAAA 
ACGTGCTGTT GGAGCAAAGC AACTGTCCTT AATATCGCCA CTTTGGCAGA AGCCAAATGC 
TTTTTTGCTC ACGATGGAGT CCAGGCCCTG CTGACCAAAG TCTGGTGGGG AGCCATGAGG 
ACAGACACCT CCATATCTAG ACTCGTGCTT ACTTTCTTCA TCCCTCCCCT GGTTTGGACC 
AGCCTTATCA AGTTCAATCC AGAGGAACAA GTGAGCAAAG ATGAAGGGGA GCCGTTTGCT 
GAACTGGACA GTTTGGAAAC AGAACAAGCA CTGTTACTCA CAGACGGGGA TCCTGTTGCT 
GGGGAAGGTA GTGCTGAAAC TGCTGCTCGC AGCTGCAGCG CAACCTTCAT CCGTGTGGTT 
CTGCGCCGCT GGAACCGTTT CTGGAGCGCT CCAGTCACCG TGTTCATGGG AAACGTCATC 
ATGTACTTTG CTTTCCTCAT TCTGTTCAGT TACGTGCTGC TTTTGGACTT CAGGCCTCCA 
CCACCGTATG GCCCCTCTGC AGCTGAGATC ATCCTCTACT TCTGGGTCTT TACCCTGGTG 
TTGGAGGAAA TCAGACAGAG TTTCTTCACG GATGAGGACA TGAGTATTCT GAAGAAGATG 
AAACTGTATG TGGAGGACAA CTGGAACAAA TGTGACATGG TGGCCATCTC CCTCTTTGTG 
GTGGGATTGT CATGCAGGAT GGCCATGAGT ACATATGAGG CTGGTCGCAC TGTTCTTGCC 
TTGGACTTCA TGGTTTTCAC CCTAAGACTC ATTCACATTT TTGCCATCCA TAAACAGCTG 
GGACCCAAAA TAATCATTGT TGAGAGAATG ATCAAGGATG TGTTCTTCTT CCTCTTCTTC 
CTGAGTGTTT GGCTGATAGC GTATGGAGTG ACGACACAGG CCCTTCTCCA CCCCAATGAC 
CCGCGCATTG ATTGGGTCTT CCGCAGGGCG CTGTACCGCC CATATCTCCA TATATTTGGA 
CAGATTCCTT TGGAGGAAAT AGATGCTGCA AAAATGCCAG ATGATAACTG CACTACTGAC 
GTGCAGGAGA TTATATTAGG CACTCTGCCA CCGTGCCCAA ACATATATGC CAACTGGCTG 
GTCATTCTGC TGCTCGTCAT CTATTTGCTG GTCACAAATG TACTGCTGCT CAACCTTCTG 
ATTGCCATGT TTAGCTACAC GTTCCAAGTG GTGCAGGAGA ATGCAGATAT CTTCTGGAAG 
TTTCAGCGCT ACAACTTAAT TGTTGAATAC CACAGCCGCC CGGCACTAGC ACCACCTTTC 
ATCATCATCA GCCACATCAC ACAGGCTCTC CTCAGCTTCA TCAAAAAGAC AGAAAACACA 
CAGGATCTGC TGGGCAAGGA ACTGCCGTCT GGCCTGGACC AGAAGCTCAT GACATGGGAG 
ACGGTGCAGA AAGAGAACTA TCTGGCTAAA CTGGAGCATG AGCACAGAGA GAGCAGCGGA 
GAGAGACTCA GATACACTTC CTCAAAGGTA CAGACTTTGC TGAGGATGGT TGGGGGTTTT 
AAGGACCAAG AAAAGCGAAT GGCAACAGTG GAGACTGAGG TGAGGTATTG TGGAGAGGTC 
TTGTCTTGGA TTGCTGAATG TTTCCACAAA AGTACTCTGA AGTGTGACAG AGACGCACCA 
AAAGCCCCAC GGTCCATTGC CGGGAGCTCC AGAGACCAGC AGCCACAAGG GGCCAAACGC 
CAGCAGCCAG CAGGACATCC AGCATATGGC ACTGACAAGA AACTGCCTTT CATTGATCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121711.1
CDS	1..3483
Translation	MVEKSSERFDKQMAGRLGDIDFTGVSRTRGKFVRVTSSTDPAEIYQILTKQWGLAPPHLVVALMGGDEVAQLKPWLRDTLRKGLVKAAQSTGAWILTSGLRFGITKNLGQAVRDHSLASTSPKVRVVAIGIAPWNMIQNRDLLLSAKPDHPATYPTEDLPYGAVYSLDCNHSHFILVDEDPKRPGATGEMRVKMLKHISLQRTGYGGTGSIEIPVLCLLVHGEPRILQKMYKNIQNSIPWLILAGSGGVADILVTLMDRGCWDADIVQELLINTFPDGLHSTEITSWTKLIQRILDHGHLLTVHDPEQDSELDTVILKALVKACKSQSQEAQDFLDELKLAVAWNRVDIAKSEIFSGDVQWSAQDLEEVMMEALVNDKPDFVRLFVDNGVNIKQFLTYGRLQELYCSVSEKNLLHTLLLKKNQERQAQLARKRMSGNPNNELGDRKFRFTFHEVSKVLKDFLDDTCKGFYQKLPAVSKALHQKNLPDMDRRCEHPWRDLFLWAILQNRQEMANYFWAMGPEAVAAALVGCKIMKEMAHLATEAESARSMKNAKYEQFAMDLFSECYSNSEDRAYSLLVRKTCCWSKATVLNIATLAEAKCFFAHDGVQALLTKVWWGAMRTDTSISRLVLTFFIPPLVWTSLIKFNPEEQVSKDEGEPFAELDSLETEQALLLTDGDPVAGEGSAETAARSCSATFIRVVLRRWNRFWSAPVTVFMGNVIMYFAFLILFSYVLLLDFRPPPPYGPSAAEIILYFWVFTLVLEEIRQSFFTDEDMSILKKMKLYVEDNWNKCDMVAISLFVVGLSCRMAMSTYEAGRTVLALDFMVFTLRLIHIFAIHKQLGPKIIIVERMIKDVFFFLFFLSVWLIAYGVTTQALLHPNDPRIDWVFRRALYRPYLHIFGQIPLEEIDAAKMPDDNCTTDVQEIILGTLPPCPNIYANWLVILLLVIYLLVTNVLLLNLLIAMFSYTFQVVQENADIFWKFQRYNLIVEYHSRPALAPPFIIISHITQALLSFIKKTENTQDLLGKELPSGLDQKLMTWETVQKENYLAKLEHEHRESSGERLRYTSSKVQTLLRMVGGFKDQEKRMATVETEVRYCGEVLSWIAECFHKSTLKCDRDAPKAPRSIAGSSRDQQPQGAKRQQPAGHPAYGTDKKLPFIDH

Target ORF information:

RefSeq Version	NM_001128239.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 5 (trpm5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128239.1

ORF Insert Sequence:

ATGGTCGAGA AGTCCAGTGA GAGATTTGAT AAACAGATGG CCGGGCGATT GGGAGATATT 
GATTTTACTG GTGTCAGTCG AACCAGGGGC AAGTTCGTAA GGGTGACCAG CTCCACGGAT 
CCAGCTGAGA TCTATCAGAT TTTGACTAAG CAGTGGGGTC TGGCTCCTCC TCATCTGGTG 
GTGGCACTGA TGGGAGGTGA TGAAGTTGCT CAGCTTAAGC CCTGGCTGAG GGACACACTC 
AGGAAAGGCC TCGTGAAGGC AGCACAGAGC ACAGGGGCCT GGATCCTCAC CAGTGGTTTG 
CGCTTTGGCA TCACTAAGAA TCTGGGTCAG GCCGTAAGAG ACCACTCTCT AGCGAGCACT 
TCCCCAAAAG TGCGAGTTGT GGCTATTGGA ATTGCCCCCT GGAACATGAT TCAGAACCGA 
GATCTGCTGC TGTCTGCAAA GCCTGATCAT CCAGCAACAT ACCCAACCGA GGATCTCCCA 
TATGGCGCTG TGTACTCCTT GGACTGCAAT CATTCACATT TCATCCTGGT GGACGAGGAT 
CCCAAGAGAC CTGGAGCCAC TGGAGAGATG AGGGTCAAGA TGCTCAAACA CATCTCTCTG 
CAGCGAACGG GATATGGGGG CACTGGCAGT ATAGAAATAC CTGTTTTGTG TCTTCTGGTT 
CATGGAGAGC CAAGGATATT ACAGAAAATG TACAAGAATA TTCAGAATTC AATTCCCTGG 
CTAATACTGG CTGGCTCAGG AGGAGTAGCC GATATTTTAG TGACCCTGAT GGACAGAGGA 
TGCTGGGATG CTGATATTGT TCAAGAGCTC TTGATTAACA CCTTTCCTGA TGGTCTGCAC 
AGCACTGAAA TCACTTCTTG GACCAAACTG ATTCAGAGAA TATTGGACCA TGGTCACCTG 
TTAACAGTGC ATGACCCAGA GCAGGATTCA GAACTGGATA CAGTAATTCT CAAAGCTCTT 
GTTAAGGCGT GTAAGAGTCA GAGTCAGGAA GCGCAGGACT TCCTGGATGA GCTGAAGCTG 
GCCGTGGCCT GGAATAGAGT GGACATCGCC AAAAGTGAGA TCTTCAGTGG AGACGTCCAA 
TGGAGTGCAC AGGACTTGGA GGAGGTCATG ATGGAGGCAC TGGTGAATGA CAAGCCTGAC 
TTTGTGCGAC TTTTTGTTGA TAACGGTGTA AACATAAAGC AATTCCTGAC TTATGGCCGT 
CTCCAAGAGC TCTACTGCTC TGTGTCTGAG AAAAACCTGC TTCACACTCT GCTCCTGAAA 
AAGAATCAGG AGAGACAGGC TCAGTTGGCC AGAAAACGAA TGTCAGGAAA CCCCAACAAT 
GAACTAGGTG ACCGCAAATT TCGTTTCACC TTCCATGAGG TCTCAAAAGT CCTCAAAGAC 
TTTCTTGATG ACACCTGTAA GGGTTTCTAC CAAAAACTTC CAGCGGTGAG TAAAGCTTTA 
CACCAGAAAA ACCTTCCAGA CATGGACAGA CGATGTGAGC ACCCTTGGAG AGACCTCTTC 
CTTTGGGCTA TTCTGCAGAA TAGGCAGGAA ATGGCAAACT ACTTCTGGGC CATGGGCCCG 
GAGGCAGTGG CAGCTGCTCT GGTGGGCTGT AAGATCATGA AGGAGATGGC CCATCTGGCT 
ACTGAAGCGG AGTCTGCTCG CAGTATGAAG AATGCCAAGT ATGAGCAGTT TGCAATGGAT 
CTTTTCAGTG AGTGTTATTC AAACAGTGAA GACAGAGCCT ATTCTCTTCT GGTGAGGAAA 
ACGTGCTGTT GGAGCAAAGC AACTGTCCTT AATATCGCCA CTTTGGCAGA AGCCAAATGC 
TTTTTTGCTC ACGATGGAGT CCAGGCCCTG CTGACCAAAG TCTGGTGGGG AGCCATGAGG 
ACAGACACCT CCATATCTAG ACTCGTGCTT ACTTTCTTCA TCCCTCCCCT GGTTTGGACC 
AGCCTTATCA AGTTCAATCC AGAGGAACAA GTGAGCAAAG ATGAAGGGGA GCCGTTTGCT 
GAACTGGACA GTTTGGAAAC AGAACAAGCA CTGTTACTCA CAGACGGGGA TCCTGTTGCT 
GGGGAAGGTA GTGCTGAAAC TGCTGCTCGC AGCTGCAGCG CAACCTTCAT CCGTGTGGTT 
CTGCGCCGCT GGAACCGTTT CTGGAGCGCT CCAGTCACCG TGTTCATGGG AAACGTCATC 
ATGTACTTTG CTTTCCTCAT TCTGTTCAGT TACGTGCTGC TTTTGGACTT CAGGCCTCCA 
CCACCGTATG GCCCCTCTGC AGCTGAGATC ATCCTCTACT TCTGGGTCTT TACCCTGGTG 
TTGGAGGAAA TCAGACAGAG TTTCTTCACG GATGAGGACA TGAGTATTCT GAAGAAGATG 
AAACTGTATG TGGAGGACAA CTGGAACAAA TGTGACATGG TGGCCATCTC CCTCTTTGTG 
GTGGGATTGT CATGCAGGAT GGCCATGAGT ACATATGAGG CTGGTCGCAC TGTTCTTGCC 
TTGGACTTCA TGGTTTTCAC CCTAAGACTC ATTCACATTT TTGCCATCCA TAAACAGCTG 
GGACCCAAAA TAATCATTGT TGAGAGAATG ATCAAGGATG TGTTCTTCTT CCTCTTCTTC 
CTGAGTGTTT GGCTGATAGC GTATGGAGTG ACGACACAGG CCCTTCTCCA CCCCAATGAC 
CCGCGCATTG ATTGGGTCTT CCGCAGGGCG CTGTACCGCC CATATCTCCA TATATTTGGA 
CAGATTCCTT TGGAGGAAAT AGATGCTGCA AAAATGCCAG ATGATAACTG CACTACTGAC 
GTGCAGGAGA TTATATTAGG CACTCTGCCA CCGTGCCCAA ACATATATGC CAACTGGCTG 
GTCATTCTGC TGCTCGTCAT CTATTTGCTG GTCACAAATG TACTGCTGCT CAACCTTCTG 
ATTGCCATGT TTAGCTACAC GTTCCAAGTG GTGCAGGAGA ATGCAGATAT CTTCTGGAAG 
TTTCAGCGCT ACAACTTAAT TGTTGAATAC CACAGCCGCC CGGCACTAGC ACCACCTTTC 
ATCATCATCA GCCACATCAC ACAGGCTCTC CTCAGCTTCA TCAAAAAGAC AGAAAACACA 
CAGGATCTGC TGGGCAAGGA ACTGCCGTCT GGCCTGGACC AGAAGCTCAT GACATGGGAG 
ACGGTGCAGA AAGAGAACTA TCTGGCTAAA CTGGAGCATG AGCACAGAGA GAGCAGCGGA 
GAGAGACTCA GATACACTTC CTCAAAGGTA CAGACTTTGC TGAGGATGGT TGGGGGTTTT 
AAGGACCAAG AAAAGCGAAT GGCAACAGTG GAGACTGAGG TGAGGTATTG TGGAGAGGTC 
TTGTCTTGGA TTGCTGAATG TTTCCACAAA AGTACTCTGA AGTGTGACAG AGACGCACCA 
AAAGCCCCAC GGTCCATTGC CGGGAGCTCC AGAGACCAGC AGCCACAAGG GGCCAAACGC 
CAGCAGCCAG CAGGACATCC AGCATATGGC ACTGACAAGA AACTGCCTTT CATTGATCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm5 ( XM_017356874.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212363.1 Danio rerio transient receptor potential cation channel, subfamily M, member 5 (trpm5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa59635
Clone ID Related Accession (Same CDS sequence)	XM_017356874.1
Accession Version	XM_017356874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCGAGA AGTCCAGTGA GAGATTTGAT AAACAGATGG CCGGGCGATT GGGAGATATT 
GATTTTACTG GTGTCAGTCG AACCAGGGGC AAGTTCGTAA GGGTGACCAG CTCCACGGAT 
CCAGCTGAGA TCTATCAGAT TTTGACTAAG CAGTGGGGTC TGGCTCCTCC TCATCTGGTG 
GTGGCACTGA TGGGAGGTGA TGAAGTTGCT CAGCTTAAGC CCTGGCTGAG GGACACACTC 
AGGAAAGGCC TCGTGAAGGC AGCACAGAGC ACAGGGGCCT GGATCCTCAC CAGTGGTTTG 
CGCTTTGGCA TCACTAAGAA TCTGGGTCAG GCCGTAAGAG ACCACTCTCT AGCGAGCACT 
TCCCCAAAAG TGCGAGTTGT GGCTATTGGA ATTGCCCCCT GGAACATGAT TCAGAACCGA 
GATCTGCTGC TGTCTGCAAA GCCTGATCAT CCAGCAACAT ACCCAACCGA GGATCTCCCA 
TATGGCGCTG TGTACTCCTT GGACTGCAAT CATTCACATT TCATCCTGGT GGACGAGGAT 
CCCAAGAGAC CTGGAGCCAC TGGAGAGATG AGGGTCAAGA TGCTCAAACA CATCTCTCTG 
CAGCGAACGG GATATGGGGG CACTGGCAGT ATAGAAATAC CTGTTTTGTG TCTTCTGGTT 
CATGGAGAGC CAAGGATATT ACAGAAAATG TACAAGAATA TTCAGAATTC AATTCCCTGG 
CTAATACTGG CTGGCTCAGG AGGAGTAGCC GATATTTTAG TGACCCTGAT GGACAGAGGA 
TGCTGGGATG CTGATATTGT TCAAGAGCTC TTGATTAACA CCTTTCCTGA TGGTCTGCAC 
AGCACTGAAA TCACTTCTTG GACCAAACTG ATTCAGAGAA TATTGGACCA TGGTCACCTG 
TTAACAGTGC ATGACCCAGA GCAGGATTCA GAACTGGATA CAGTAATTCT CAAAGCTCTT 
GTTAAGGCGT GTAAGAGTCA GAGTCAGGAA GCGCAGGACT TCCTGGATGA GCTGAAGCTG 
GCCGTGGCCT GGAATAGAGT GGACATCGCC AAAAGTGAGA TCTTCAGTGG AGACGTCCAA 
TGGAGTGCAC AGGACTTGGA GGAGGTCATG ATGGAGGCAC TGGTGAATGA CAAGCCTGAC 
TTTGTGCGAC TTTTTGTTGA TAACGGTGTA AACATAAAGC AATTCCTGAC TTATGGCCGT 
CTCCAAGAGC TCTACTGCTC TGTGTCTGAG AAAAACCTGC TTCACACTCT GCTCCTGAAA 
AAGAATCAGG AGAGACAGGC TCAGTTGGCC AGAAAACGAA TGTCAGGAAA CCCCAACAAT 
GAACTAGGTG ACCGCAAATT TCGTTTCACC TTCCATGAGG TCTCAAAAGT CCTCAAAGAC 
TTTCTTGATG ACACCTGTAA GGGTTTCTAC CAAAAACTTC CAGCGGAAAG GATGGGAAAA 
GGCAGACTTT TCCACAGCCA GAAAAACCTT CCAGACATGG ACAGACGATG TGAGCACCCT 
TGGAGAGACC TCTTCCTTTG GGCTATTCTG CAGAATAGGC AGGAAATGGC AAACTACTTC 
TGGGCCATGG GCCCGGAGGC AGTGGCAGCT GCTCTGGTGG GCTGTAAGAT CATGAAGGAG 
ATGGCCCATC TGGCTACTGA AGCGGAGTCT GCTCGCAGTA TGAAGAATGC CAAGTATGAG 
CAGTTTGCAA TGGATCTTTT CAGTGAGTGT TATTCAAACA GTGAAGACAG AGCCTATTCT 
CTTCTGGTGA GGAAAACGTG CTGTTGGAGC AAAGCAACTG TCCTTAATAT CGCCACTTTG 
GCAGAAGCCA AATGCTTTTT TGCTCACGAT GGAGTCCAGG CCCTGCTGAC CAAAGTCTGG 
TGGGGAGCCA TGAGGACAGA CACCTCCATA TCTAGACTCG TGCTTACTTT CTTCATCCCT 
CCCCTGGTTT GGACCAGCCT TATCAAGTTC AATCCAGAGG AACAAGTGAG CAAAGATGAA 
GGGGAGCCGT TTGCTGAACT GGACAGTTTG GAAACAGAAC AAGCACTGTT ACTCACAGAC 
GGGGATCCTG TTGCTGGGGA AGGTAGTGCT GAAACTGCTG CTCGCAGCTG CAGCGCAACC 
TTCATCCGTG TGGTTCTGCG CCGCTGGAAC CGTTTCTGGA GCGCTCCAGT CACCGTGTTC 
ATGGGAAACG TCATCATGTA CTTTGCTTTC CTCATTCTGT TCAGTTACGT GCTGCTTTTG 
GACTTCAGGC CTCCACCACC GTATGGCCCC TCTGCAGCTG AGATCATCCT CTACTTCTGG 
GTCTTTACCC TGGTGTTGGA GGAAATCAGA CAGAGTTTCT TCACGGATGA GGACATGAGT 
ATTCTGAAGA AGATGAAACT GTATGTGGAG GACAACTGGA ACAAATGTGA CATGGTGGCC 
ATCTCCCTCT TTGTGGTGGG ATTGTCATGC AGGATGGCCA TGAGTACATA TGAGGCTGGT 
CGCACTGTTC TTGCCTTGGA CTTCATGGTT TTCACCCTAA GACTCATTCA CATTTTTGCC 
ATCCATAAAC AGCTGGGACC CAAAATAATC ATTGTTGAGA GAATGATCAA GGATGTGTTC 
TTCTTCCTCT TCTTCCTGAG TGTTTGGCTG ATAGCGTATG GAGTGACGAC ACAGGCCCTT 
CTCCACCCCA ATGACCCGCG CATTGATTGG GTCTTCCGCA GGGCGCTGTA CCGCCCATAT 
CTCCATATAT TTGGACAGAT TCCTTTGGAG GAAATAGATG CTGCAAAAAT GCCAGATGAT 
AACTGCACTA CTGACGTGCA GGAGATTATA TTAGGCACTC TGCCACCGTG CCCAAACATA 
TATGCCAACT GGCTGGTCAT TCTGCTGCTC GTCATCTATT TGCTGGTCAC AAATGTACTG 
CTGCTCAACC TTCTGATTGC CATGTTTAGC TACACGTTCC AAGTGGTGCA GGAGAATGCA 
GATATCTTCT GGAAGTTTCA GCGCTACAAC TTAATTGTTG AATACCACAG CCGCCCGGCA 
CTAGCACCAC CTTTCATCAT CATCAGCCAC ATCACACAGG CTCTCCTCAG CTTCATCAAA 
AAGACAGAAA ACACACAGGA TCTGCTGGAG AGGGAACTGC CGTCTGGCCT GGACCAGAAG 
CTCATGACAT GGGAGACGGT GCAGAAAGAG AACTATCTGG CTAAACTGGA GCATGAGCAC 
AGAGAGAGCA GCGGAGAGAG ACTCAGATAC ACTTCCTCAA AGGTACAGAC TTTGCTGAGG 
ATGGTTGGGG GTTTTAAGGA CCAAGAAAAG CGAATGGCAA CAGTGGAGAC TGAGGTGAGG 
TATTGTGGAG AGGTCTTGTC TTGGATTGCT GAATGTTTCC ACAAAAGTAC TCTGAAGTGT 
GACAGAGACG CACCAAAAGC CCCACGGTCC ATTGCCGGGA GCTCCAGAGA CCAGCAGCCA 
CAAGGGGCCA AACGCCAGCA GCCAGCAGGA CATCCAGCAT ATGGCACTGA CAAGAAACTG 
CCTTTCATTG ATCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212363.1
CDS	41..3538
Translation	MVEKSSERFDKQMAGRLGDIDFTGVSRTRGKFVRVTSSTDPAEIYQILTKQWGLAPPHLVVALMGGDEVAQLKPWLRDTLRKGLVKAAQSTGAWILTSGLRFGITKNLGQAVRDHSLASTSPKVRVVAIGIAPWNMIQNRDLLLSAKPDHPATYPTEDLPYGAVYSLDCNHSHFILVDEDPKRPGATGEMRVKMLKHISLQRTGYGGTGSIEIPVLCLLVHGEPRILQKMYKNIQNSIPWLILAGSGGVADILVTLMDRGCWDADIVQELLINTFPDGLHSTEITSWTKLIQRILDHGHLLTVHDPEQDSELDTVILKALVKACKSQSQEAQDFLDELKLAVAWNRVDIAKSEIFSGDVQWSAQDLEEVMMEALVNDKPDFVRLFVDNGVNIKQFLTYGRLQELYCSVSEKNLLHTLLLKKNQERQAQLARKRMSGNPNNELGDRKFRFTFHEVSKVLKDFLDDTCKGFYQKLPAERMGKGRLFHSQKNLPDMDRRCEHPWRDLFLWAILQNRQEMANYFWAMGPEAVAAALVGCKIMKEMAHLATEAESARSMKNAKYEQFAMDLFSECYSNSEDRAYSLLVRKTCCWSKATVLNIATLAEAKCFFAHDGVQALLTKVWWGAMRTDTSISRLVLTFFIPPLVWTSLIKFNPEEQVSKDEGEPFAELDSLETEQALLLTDGDPVAGEGSAETAARSCSATFIRVVLRRWNRFWSAPVTVFMGNVIMYFAFLILFSYVLLLDFRPPPPYGPSAAEIILYFWVFTLVLEEIRQSFFTDEDMSILKKMKLYVEDNWNKCDMVAISLFVVGLSCRMAMSTYEAGRTVLALDFMVFTLRLIHIFAIHKQLGPKIIIVERMIKDVFFFLFFLSVWLIAYGVTTQALLHPNDPRIDWVFRRALYRPYLHIFGQIPLEEIDAAKMPDDNCTTDVQEIILGTLPPCPNIYANWLVILLLVIYLLVTNVLLLNLLIAMFSYTFQVVQENADIFWKFQRYNLIVEYHSRPALAPPFIIISHITQALLSFIKKTENTQDLLERELPSGLDQKLMTWETVQKENYLAKLEHEHRESSGERLRYTSSKVQTLLRMVGGFKDQEKRMATVETEVRYCGEVLSWIAECFHKSTLKCDRDAPKAPRSIAGSSRDQQPQGAKRQQPAGHPAYGTDKKLPFIDH

Target ORF information:

RefSeq Version	XM_017356874.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 5 (trpm5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356874.1

ORF Insert Sequence:

ATGGTCGAGA AGTCCAGTGA GAGATTTGAT AAACAGATGG CCGGGCGATT GGGAGATATT 
GATTTTACTG GTGTCAGTCG AACCAGGGGC AAGTTCGTAA GGGTGACCAG CTCCACGGAT 
CCAGCTGAGA TCTATCAGAT TTTGACTAAG CAGTGGGGTC TGGCTCCTCC TCATCTGGTG 
GTGGCACTGA TGGGAGGTGA TGAAGTTGCT CAGCTTAAGC CCTGGCTGAG GGACACACTC 
AGGAAAGGCC TCGTGAAGGC AGCACAGAGC ACAGGGGCCT GGATCCTCAC CAGTGGTTTG 
CGCTTTGGCA TCACTAAGAA TCTGGGTCAG GCCGTAAGAG ACCACTCTCT AGCGAGCACT 
TCCCCAAAAG TGCGAGTTGT GGCTATTGGA ATTGCCCCCT GGAACATGAT TCAGAACCGA 
GATCTGCTGC TGTCTGCAAA GCCTGATCAT CCAGCAACAT ACCCAACCGA GGATCTCCCA 
TATGGCGCTG TGTACTCCTT GGACTGCAAT CATTCACATT TCATCCTGGT GGACGAGGAT 
CCCAAGAGAC CTGGAGCCAC TGGAGAGATG AGGGTCAAGA TGCTCAAACA CATCTCTCTG 
CAGCGAACGG GATATGGGGG CACTGGCAGT ATAGAAATAC CTGTTTTGTG TCTTCTGGTT 
CATGGAGAGC CAAGGATATT ACAGAAAATG TACAAGAATA TTCAGAATTC AATTCCCTGG 
CTAATACTGG CTGGCTCAGG AGGAGTAGCC GATATTTTAG TGACCCTGAT GGACAGAGGA 
TGCTGGGATG CTGATATTGT TCAAGAGCTC TTGATTAACA CCTTTCCTGA TGGTCTGCAC 
AGCACTGAAA TCACTTCTTG GACCAAACTG ATTCAGAGAA TATTGGACCA TGGTCACCTG 
TTAACAGTGC ATGACCCAGA GCAGGATTCA GAACTGGATA CAGTAATTCT CAAAGCTCTT 
GTTAAGGCGT GTAAGAGTCA GAGTCAGGAA GCGCAGGACT TCCTGGATGA GCTGAAGCTG 
GCCGTGGCCT GGAATAGAGT GGACATCGCC AAAAGTGAGA TCTTCAGTGG AGACGTCCAA 
TGGAGTGCAC AGGACTTGGA GGAGGTCATG ATGGAGGCAC TGGTGAATGA CAAGCCTGAC 
TTTGTGCGAC TTTTTGTTGA TAACGGTGTA AACATAAAGC AATTCCTGAC TTATGGCCGT 
CTCCAAGAGC TCTACTGCTC TGTGTCTGAG AAAAACCTGC TTCACACTCT GCTCCTGAAA 
AAGAATCAGG AGAGACAGGC TCAGTTGGCC AGAAAACGAA TGTCAGGAAA CCCCAACAAT 
GAACTAGGTG ACCGCAAATT TCGTTTCACC TTCCATGAGG TCTCAAAAGT CCTCAAAGAC 
TTTCTTGATG ACACCTGTAA GGGTTTCTAC CAAAAACTTC CAGCGGAAAG GATGGGAAAA 
GGCAGACTTT TCCACAGCCA GAAAAACCTT CCAGACATGG ACAGACGATG TGAGCACCCT 
TGGAGAGACC TCTTCCTTTG GGCTATTCTG CAGAATAGGC AGGAAATGGC AAACTACTTC 
TGGGCCATGG GCCCGGAGGC AGTGGCAGCT GCTCTGGTGG GCTGTAAGAT CATGAAGGAG 
ATGGCCCATC TGGCTACTGA AGCGGAGTCT GCTCGCAGTA TGAAGAATGC CAAGTATGAG 
CAGTTTGCAA TGGATCTTTT CAGTGAGTGT TATTCAAACA GTGAAGACAG AGCCTATTCT 
CTTCTGGTGA GGAAAACGTG CTGTTGGAGC AAAGCAACTG TCCTTAATAT CGCCACTTTG 
GCAGAAGCCA AATGCTTTTT TGCTCACGAT GGAGTCCAGG CCCTGCTGAC CAAAGTCTGG 
TGGGGAGCCA TGAGGACAGA CACCTCCATA TCTAGACTCG TGCTTACTTT CTTCATCCCT 
CCCCTGGTTT GGACCAGCCT TATCAAGTTC AATCCAGAGG AACAAGTGAG CAAAGATGAA 
GGGGAGCCGT TTGCTGAACT GGACAGTTTG GAAACAGAAC AAGCACTGTT ACTCACAGAC 
GGGGATCCTG TTGCTGGGGA AGGTAGTGCT GAAACTGCTG CTCGCAGCTG CAGCGCAACC 
TTCATCCGTG TGGTTCTGCG CCGCTGGAAC CGTTTCTGGA GCGCTCCAGT CACCGTGTTC 
ATGGGAAACG TCATCATGTA CTTTGCTTTC CTCATTCTGT TCAGTTACGT GCTGCTTTTG 
GACTTCAGGC CTCCACCACC GTATGGCCCC TCTGCAGCTG AGATCATCCT CTACTTCTGG 
GTCTTTACCC TGGTGTTGGA GGAAATCAGA CAGAGTTTCT TCACGGATGA GGACATGAGT 
ATTCTGAAGA AGATGAAACT GTATGTGGAG GACAACTGGA ACAAATGTGA CATGGTGGCC 
ATCTCCCTCT TTGTGGTGGG ATTGTCATGC AGGATGGCCA TGAGTACATA TGAGGCTGGT 
CGCACTGTTC TTGCCTTGGA CTTCATGGTT TTCACCCTAA GACTCATTCA CATTTTTGCC 
ATCCATAAAC AGCTGGGACC CAAAATAATC ATTGTTGAGA GAATGATCAA GGATGTGTTC 
TTCTTCCTCT TCTTCCTGAG TGTTTGGCTG ATAGCGTATG GAGTGACGAC ACAGGCCCTT 
CTCCACCCCA ATGACCCGCG CATTGATTGG GTCTTCCGCA GGGCGCTGTA CCGCCCATAT 
CTCCATATAT TTGGACAGAT TCCTTTGGAG GAAATAGATG CTGCAAAAAT GCCAGATGAT 
AACTGCACTA CTGACGTGCA GGAGATTATA TTAGGCACTC TGCCACCGTG CCCAAACATA 
TATGCCAACT GGCTGGTCAT TCTGCTGCTC GTCATCTATT TGCTGGTCAC AAATGTACTG 
CTGCTCAACC TTCTGATTGC CATGTTTAGC TACACGTTCC AAGTGGTGCA GGAGAATGCA 
GATATCTTCT GGAAGTTTCA GCGCTACAAC TTAATTGTTG AATACCACAG CCGCCCGGCA 
CTAGCACCAC CTTTCATCAT CATCAGCCAC ATCACACAGG CTCTCCTCAG CTTCATCAAA 
AAGACAGAAA ACACACAGGA TCTGCTGGAG AGGGAACTGC CGTCTGGCCT GGACCAGAAG 
CTCATGACAT GGGAGACGGT GCAGAAAGAG AACTATCTGG CTAAACTGGA GCATGAGCAC 
AGAGAGAGCA GCGGAGAGAG ACTCAGATAC ACTTCCTCAA AGGTACAGAC TTTGCTGAGG 
ATGGTTGGGG GTTTTAAGGA CCAAGAAAAG CGAATGGCAA CAGTGGAGAC TGAGGTGAGG 
TATTGTGGAG AGGTCTTGTC TTGGATTGCT GAATGTTTCC ACAAAAGTAC TCTGAAGTGT 
GACAGAGACG CACCAAAAGC CCCACGGTCC ATTGCCGGGA GCTCCAGAGA CCAGCAGCCA 
CAAGGGGCCA AACGCCAGCA GCCAGCAGGA CATCCAGCAT ATGGCACTGA CAAGAAACTG 
CCTTTCATTG ATCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Thermal stress in Danio rerio: a link between temperature, light, thermo-TRP channels, and clock genes.
Journal of thermal biology68(Pt A)128-138(2017 Aug)
Jerônimo R,Moraes MN,de Assis LVM,Ramos BC,Rocha T,Castrucci AML

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Phylogenetic analysis and expression of zebrafish transient receptor potential melastatin family genes.
Developmental dynamics : an official publication of the American Association of Anatomists242(11)1236-49(2013 Nov)
Kastenhuber E,Gesemann M,Mickoleit M,Neuhauss SC

Epidermal keratinocyte polarity and motility require Ca²⁺ influx through TRPV1.
Journal of cell science126(Pt 20)4602-13(2013 Oct)
Graham DM,Huang L,Robinson KR,Messerli MA

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trpm5 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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