Alg2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alg2
Entrez Gene ID	56737
Full Name	asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase)
Synonyms	1110018A23Rik,1300013N08Rik,ALPG2,CDGIi,MNCb-5081
General protein information	Preferred Names asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase) Names alpha-1,3/1,6-mannosyltransferase ALG2 GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase alpha-1,3-mannosyltransferase ALG2 asparagine-linked glycosylation 2 homolog (yeast, alpha-1,3-mannosyltransferase) asparagine-linked glycosylation protein 2 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001355496.1	NP_001342425.1	alpha-1,3/1,6-mannosyltransferase ALG2 isoform 2
XM_006538124.3	XP_006538187.1	alpha-1,3/1,6-mannosyltransferase ALG2 isoform X1
NM_019998.3	NP_064382.3	alpha-1,3/1,6-mannosyltransferase ALG2 isoform 1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	ALG2	XP_001112564.1
Caenorhabditis elegans (roundworm)	F09E5.2	NP_495010.2
Magnaporthe oryzae (rice blast fungus)	MGG_02384	XP_003709160.1
Schizosaccharomyces pombe (fission yeast)	alg2	NP_595621.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001232	XP_321925.2
Homo sapiens (human)	ALG2	NP_149078.1
Pan troglodytes (chimpanzee)	ALG2	NP_001233488.1
Canis lupus familiaris (dog)	ALG2	XP_532010.2
Mus musculus (house mouse)	Alg2	NP_064382.3
Gallus gallus (chicken)	ALG2	XP_003640838.1
Saccharomyces cerevisiae (baker's yeast)	ALG2	NP_011450.1
Arabidopsis thaliana (thale cress)	AT1G78800	NP_178001.2
Neurospora crassa	NCU03503	XP_955840.1
Bos taurus (cattle)	ALG2	NP_001076960.1
Xenopus tropicalis (tropical clawed frog)	alg2	NP_001025660.1
Kluyveromyces lactis	KLLA0B02420g	XP_451639.1
Rattus norvegicus (Norway rat)	Alg2	NP_001094180.1
Danio rerio (zebrafish)	alg2	NP_001098406.2
Drosophila melanogaster (fruit fly)	CG1291	NP_647772.1
Oryza sativa (rice)	Os04g0589600	NP_001053699.1
Eremothecium gossypii	AGOS_AFL098W	NP_985452.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

ALG-2 divalent-ion affinity: Calorimetric analysis of the des23 versions reveals high-affinity site for Mg(2).
Henzl MT, Frey BB, Wolf AJ
Biophysical chemistry20928-40(2016 Feb)

Human immunodeficiency virus type 1 enhancer-binding protein 3 is essential for the expression of asparagine-linked glycosylation 2 in the regulation of osteoblast and chondrocyte differentiation.
Imamura K, Maeda S, Kawamura I, Matsuyama K, Shinohara N, Yahiro Y, Nagano S, Setoguchi T, Yokouchi M, Ishidou Y, Komiya S
The Journal of biological chemistry289(14)9865-79(2014 Apr)

A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ
Nature biotechnology28(7)749-55(2010 Jul)

The calcium binding protein ALG-2 binds and stabilizes Scotin, a p53-inducible gene product localized at the endoplasmic reticulum membrane.
Draeby I, Woods YL, la Cour JM, Mollerup J, Bourdon JC, Berchtold MW
Archives of biochemistry and biophysics467(1)87-94(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Alg2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alg2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu33068	NM_001355496.1 Latest version!	Mus musculus asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase) (Alg2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu33068	XM_006538124.3 Latest version!	Mus musculus asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase) (Alg2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu11869	NM_019998.3 Latest version!	Mus musculus asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase) (Alg2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alg2 ( NM_001355496.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342425.1 Mus musculus asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase) (Alg2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu33068
Clone ID Related Accession (Same CDS sequence)	NM_001355496.1 , XM_006538124.3
Accession Version	NM_001355496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1538150400000
Organism	Mus musculus(house mouse)
Product	alpha-1,3/1,6-mannosyltransferase ALG2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY129752.1, BC052411.1, BU612370.1 and AI834815.1. On Sep 20, 2017 this sequence version replaced XM_006538124.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) has the same N- and C-termini, but is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.156266.1, SRR1660815.125101.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGCG CACTACGACC CGAACCACTG CTTCATCGAG ACCCGCGAGC TCTCGGTGCA 
ATGCGCAGGG GACTGGCTGC CTCGCAGCCT GGGCTGGGGC GGCCGCGGCG CCGCCATCTG 
CTCCTACGTG CGCATGGTCT TTCTGGCGCT CTACGTGCTG TTTCTCTCCG GCGAGGAGTT 
CGACGTGGTG GTGTGCGACC AGATCTGCTG CTTACTCAGA GAAATTCAGC TCTGAAGAAG 
TTCTACAGGG CCCCCATCGA CTGGATCGAG GAATACACCA CAGGCATGGC AGACCGCATC 
TTGGTCAACA GCCAGTACAC TGCTTCCGTC TTTAAAGAAA CCTTCAAGAC CCTGTCTCAC 
AGAAATCCTG ATGTGCTCTA CCCATCTCTG AATATCGGCA GCTTTGACTT GGCTATTCCT 
GAAAAGATAG ATGACCTCGT CCCCAAGGGG AAGCAATTCC TGTTCCTCTC TATCAACCGA 
TACGAAAGGA AGAAAAATCT GCCCTTGGCA CTGAGATCCT TGGTGCAGCT TCGGAATCGG 
TTACCATCTC AAGAGTGGGA TAAGGTTCAT CTCTTCATGG CCGGTGGTTA TGACGATAGG 
ATCCCGGAGA ACGTGGAGCA CTATAAGGAG TTGAAGAAAA TGGTCCAAGA GTCAGACCTT 
GAGCGTCATG TGACCTTCCT GCGGTCCTTC TCGGACAGAC AGAAGATCTC ACTCCTCCAC 
GGCTGCTTGT GTGTGCTCTA CACTCCGAGC AACGAGCACT TTGGCATCGT CCCTCTGGAG 
GCCATGTACA TGCAGTGCCC AGTCATCGCT GTTAATAACG GTGGGCCCCT GGAGTCCATT 
GTCCACAAGG TCACGGGGTT CCTGTGTGAG CCAGACCCAG TGCACTTCTC AGAAGCCATG 
GAGAAGTTCA TCCACAAACC ATCCTTAAAA GCCACGATGG GCCTCGCTGG AAAAGCCAGG 
GTGGCGGAGA AGTTTTCAGC TGATGCATTT GCAGACCAGC TGTACCAGTA TGTCACGAAG 
CTGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342425.1
CDS	228..1256
Translation	MDRALRPEPLLHRDPRALGAMRRGLAASQPGLGRPRRRHLLLRAHGLSGALRAVSLRRGVRRGGVRPDLLLTQRNSALKKFYRAPIDWIEEYTTGMADRILVNSQYTASVFKETFKTLSHRNPDVLYPSLNIGSFDLAIPEKIDDLVPKGKQFLFLSINRYERKKNLPLALRSLVQLRNRLPSQEWDKVHLFMAGGYDDRIPENVEHYKELKKMVQESDLERHVTFLRSFSDRQKISLLHGCLCVLYTPSNEHFGIVPLEAMYMQCPVIAVNNGGPLESIVHKVTGFLCEPDPVHFSEAMEKFIHKPSLKATMGLAGKARVAEKFSADAFADQLYQYVTKLV

Target ORF information:

RefSeq Version	NM_001355496.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase) (Alg2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355496.1

ORF Insert Sequence:

ATGGACCGCG CACTACGACC CGAACCACTG CTTCATCGAG ACCCGCGAGC TCTCGGTGCA 
ATGCGCAGGG GACTGGCTGC CTCGCAGCCT GGGCTGGGGC GGCCGCGGCG CCGCCATCTG 
CTCCTACGTG CGCATGGTCT TTCTGGCGCT CTACGTGCTG TTTCTCTCCG GCGAGGAGTT 
CGACGTGGTG GTGTGCGACC AGATCTGCTG CTTACTCAGA GAAATTCAGC TCTGAAGAAG 
TTCTACAGGG CCCCCATCGA CTGGATCGAG GAATACACCA CAGGCATGGC AGACCGCATC 
TTGGTCAACA GCCAGTACAC TGCTTCCGTC TTTAAAGAAA CCTTCAAGAC CCTGTCTCAC 
AGAAATCCTG ATGTGCTCTA CCCATCTCTG AATATCGGCA GCTTTGACTT GGCTATTCCT 
GAAAAGATAG ATGACCTCGT CCCCAAGGGG AAGCAATTCC TGTTCCTCTC TATCAACCGA 
TACGAAAGGA AGAAAAATCT GCCCTTGGCA CTGAGATCCT TGGTGCAGCT TCGGAATCGG 
TTACCATCTC AAGAGTGGGA TAAGGTTCAT CTCTTCATGG CCGGTGGTTA TGACGATAGG 
ATCCCGGAGA ACGTGGAGCA CTATAAGGAG TTGAAGAAAA TGGTCCAAGA GTCAGACCTT 
GAGCGTCATG TGACCTTCCT GCGGTCCTTC TCGGACAGAC AGAAGATCTC ACTCCTCCAC 
GGCTGCTTGT GTGTGCTCTA CACTCCGAGC AACGAGCACT TTGGCATCGT CCCTCTGGAG 
GCCATGTACA TGCAGTGCCC AGTCATCGCT GTTAATAACG GTGGGCCCCT GGAGTCCATT 
GTCCACAAGG TCACGGGGTT CCTGTGTGAG CCAGACCCAG TGCACTTCTC AGAAGCCATG 
GAGAAGTTCA TCCACAAACC ATCCTTAAAA GCCACGATGG GCCTCGCTGG AAAAGCCAGG 
GTGGCGGAGA AGTTTTCAGC TGATGCATTT GCAGACCAGC TGTACCAGTA TGTCACGAAG 
CTGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alg2 ( XM_006538124.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538187.1 Mus musculus asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase) (Alg2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33068
Clone ID Related Accession (Same CDS sequence)	NM_001355496.1 , XM_006538124.3
Accession Version	XM_006538124.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(House mouse)
Product	alpha-1,3/1,6-mannosyltransferase ALG2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538124.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGCG CACTACGACC CGAACCACTG CTTCATCGAG ACCCGCGAGC TCTCGGTGCA 
ATGCGCAGGG GACTGGCTGC CTCGCAGCCT GGGCTGGGGC GGCCGCGGCG CCGCCATCTG 
CTCCTACGTG CGCATGGTCT TTCTGGCGCT CTACGTGCTG TTTCTCTCCG GCGAGGAGTT 
CGACGTGGTG GTGTGCGACC AGATCTGCTG CTTACTCAGA GAAATTCAGC TCTGAAGAAG 
TTCTACAGGG CCCCCATCGA CTGGATCGAG GAATACACCA CAGGCATGGC AGACCGCATC 
TTGGTCAACA GCCAGTACAC TGCTTCCGTC TTTAAAGAAA CCTTCAAGAC CCTGTCTCAC 
AGAAATCCTG ATGTGCTCTA CCCATCTCTG AATATCGGCA GCTTTGACTT GGCTATTCCT 
GAAAAGATAG ATGACCTCGT CCCCAAGGGG AAGCAATTCC TGTTCCTCTC TATCAACCGA 
TACGAAAGGA AGAAAAATCT GCCCTTGGCA CTGAGATCCT TGGTGCAGCT TCGGAATCGG 
TTACCATCTC AAGAGTGGGA TAAGGTTCAT CTCTTCATGG CCGGTGGTTA TGACGATAGG 
ATCCCGGAGA ACGTGGAGCA CTATAAGGAG TTGAAGAAAA TGGTCCAAGA GTCAGACCTT 
GAGCGTCATG TGACCTTCCT GCGGTCCTTC TCGGACAGAC AGAAGATCTC ACTCCTCCAC 
GGCTGCTTGT GTGTGCTCTA CACTCCGAGC AACGAGCACT TTGGCATCGT CCCTCTGGAG 
GCCATGTACA TGCAGTGCCC AGTCATCGCT GTTAATAACG GTGGGCCCCT GGAGTCCATT 
GTCCACAAGG TCACGGGGTT CCTGTGTGAG CCAGACCCAG TGCACTTCTC AGAAGCCATG 
GAGAAGTTCA TCCACAAACC ATCCTTAAAA GCCACGATGG GCCTCGCTGG AAAAGCCAGG 
GTGGCGGAGA AGTTTTCAGC TGATGCATTT GCAGACCAGC TGTACCAGTA TGTCACGAAG 
CTGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538187.1
CDS	231..1259
Translation	MDRALRPEPLLHRDPRALGAMRRGLAASQPGLGRPRRRHLLLRAHGLSGALRAVSLRRGVRRGGVRPDLLLTQRNSALKKFYRAPIDWIEEYTTGMADRILVNSQYTASVFKETFKTLSHRNPDVLYPSLNIGSFDLAIPEKIDDLVPKGKQFLFLSINRYERKKNLPLALRSLVQLRNRLPSQEWDKVHLFMAGGYDDRIPENVEHYKELKKMVQESDLERHVTFLRSFSDRQKISLLHGCLCVLYTPSNEHFGIVPLEAMYMQCPVIAVNNGGPLESIVHKVTGFLCEPDPVHFSEAMEKFIHKPSLKATMGLAGKARVAEKFSADAFADQLYQYVTKLV

Target ORF information:

RefSeq Version	XM_006538124.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase) (Alg2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538124.3

ORF Insert Sequence:

ATGGACCGCG CACTACGACC CGAACCACTG CTTCATCGAG ACCCGCGAGC TCTCGGTGCA 
ATGCGCAGGG GACTGGCTGC CTCGCAGCCT GGGCTGGGGC GGCCGCGGCG CCGCCATCTG 
CTCCTACGTG CGCATGGTCT TTCTGGCGCT CTACGTGCTG TTTCTCTCCG GCGAGGAGTT 
CGACGTGGTG GTGTGCGACC AGATCTGCTG CTTACTCAGA GAAATTCAGC TCTGAAGAAG 
TTCTACAGGG CCCCCATCGA CTGGATCGAG GAATACACCA CAGGCATGGC AGACCGCATC 
TTGGTCAACA GCCAGTACAC TGCTTCCGTC TTTAAAGAAA CCTTCAAGAC CCTGTCTCAC 
AGAAATCCTG ATGTGCTCTA CCCATCTCTG AATATCGGCA GCTTTGACTT GGCTATTCCT 
GAAAAGATAG ATGACCTCGT CCCCAAGGGG AAGCAATTCC TGTTCCTCTC TATCAACCGA 
TACGAAAGGA AGAAAAATCT GCCCTTGGCA CTGAGATCCT TGGTGCAGCT TCGGAATCGG 
TTACCATCTC AAGAGTGGGA TAAGGTTCAT CTCTTCATGG CCGGTGGTTA TGACGATAGG 
ATCCCGGAGA ACGTGGAGCA CTATAAGGAG TTGAAGAAAA TGGTCCAAGA GTCAGACCTT 
GAGCGTCATG TGACCTTCCT GCGGTCCTTC TCGGACAGAC AGAAGATCTC ACTCCTCCAC 
GGCTGCTTGT GTGTGCTCTA CACTCCGAGC AACGAGCACT TTGGCATCGT CCCTCTGGAG 
GCCATGTACA TGCAGTGCCC AGTCATCGCT GTTAATAACG GTGGGCCCCT GGAGTCCATT 
GTCCACAAGG TCACGGGGTT CCTGTGTGAG CCAGACCCAG TGCACTTCTC AGAAGCCATG 
GAGAAGTTCA TCCACAAACC ATCCTTAAAA GCCACGATGG GCCTCGCTGG AAAAGCCAGG 
GTGGCGGAGA AGTTTTCAGC TGATGCATTT GCAGACCAGC TGTACCAGTA TGTCACGAAG 
CTGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alg2 ( NM_019998.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064382.3 Mus musculus asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase) (Alg2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11869
Clone ID Related Accession (Same CDS sequence)	NM_019998.3
Accession Version	NM_019998.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Mus musculus(house mouse)
Product	alpha-1,3/1,6-mannosyltransferase ALG2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY129752.1, BC052411.1 and BG093758.1. On Apr 4, 2008 this sequence version replaced NM_019998.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC051951.1, AK004997.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAAA ACCTGTACCG AGCCCGTTCC CGGGTTTACA GCCCATCTGT GCTGTTTCTG 
CACCCAGACA TGGGTATAGG CGGAGCCGAG CGCCTAGTGC TGGACGCGGC GCTGGCGCTG 
CAGGAGTACG GCTGTGATGT GAAGATATGG ACCGCGCACT ACGACCCGAA CCACTGCTTC 
ATCGAGACCC GCGAGCTCTC GGTGCAATGC GCAGGGGACT GGCTGCCTCG CAGCCTGGGC 
TGGGGCGGCC GCGGCGCCGC CATCTGCTCC TACGTGCGCA TGGTCTTTCT GGCGCTCTAC 
GTGCTGTTTC TCTCCGGCGA GGAGTTCGAC GTGGTGGTGT GCGACCAGGT GTCTGCCTGT 
ATCCCGGTGT TCAAACTGGC CAGACGGCGT AAGAGGGTCC TATTTTACTG TCACTTCCCA 
GATCTGCTGC TTACTCAGAG AAATTCAGCT CTGAAGAAGT TCTACAGGGC CCCCATCGAC 
TGGATCGAGG AATACACCAC AGGCATGGCA GACCGCATCT TGGTCAACAG CCAGTACACT 
GCTTCCGTCT TTAAAGAAAC CTTCAAGACC CTGTCTCACA GAAATCCTGA TGTGCTCTAC 
CCATCTCTGA ATATCGGCAG CTTTGACTTG GCTATTCCTG AAAAGATAGA TGACCTCGTC 
CCCAAGGGGA AGCAATTCCT GTTCCTCTCT ATCAACCGAT ACGAAAGGAA GAAAAATCTG 
CCCTTGGCAC TGAGATCCTT GGTGCAGCTT CGGAATCGGT TACCATCTCA AGAGTGGGAT 
AAGGTTCATC TCTTCATGGC CGGTGGTTAT GACGATAGGA TCCCGGAGAA CGTGGAGCAC 
TATAAGGAGT TGAAGAAAAT GGTCCAAGAG TCAGACCTTG AGCGTCATGT GACCTTCCTG 
CGGTCCTTCT CGGACAGACA GAAGATCTCA CTCCTCCACG GCTGCTTGTG TGTGCTCTAC 
ACTCCGAGCA ACGAGCACTT TGGCATCGTC CCTCTGGAGG CCATGTACAT GCAGTGCCCA 
GTCATCGCTG TTAATAACGG TGGGCCCCTG GAGTCCATTG TCCACAAGGT CACGGGGTTC 
CTGTGTGAGC CAGACCCAGT GCACTTCTCA GAAGCCATGG AGAAGTTCAT CCACAAACCA 
TCCTTAAAAG CCACGATGGG CCTCGCTGGA AAAGCCAGGG TGGCGGAGAA GTTTTCAGCT 
GATGCATTTG CAGACCAGCT GTACCAGTAT GTCACGAAGC TGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064382.3
CDS	82..1329
Translation	MAENLYRARSRVYSPSVLFLHPDMGIGGAERLVLDAALALQEYGCDVKIWTAHYDPNHCFIETRELSVQCAGDWLPRSLGWGGRGAAICSYVRMVFLALYVLFLSGEEFDVVVCDQVSACIPVFKLARRRKRVLFYCHFPDLLLTQRNSALKKFYRAPIDWIEEYTTGMADRILVNSQYTASVFKETFKTLSHRNPDVLYPSLNIGSFDLAIPEKIDDLVPKGKQFLFLSINRYERKKNLPLALRSLVQLRNRLPSQEWDKVHLFMAGGYDDRIPENVEHYKELKKMVQESDLERHVTFLRSFSDRQKISLLHGCLCVLYTPSNEHFGIVPLEAMYMQCPVIAVNNGGPLESIVHKVTGFLCEPDPVHFSEAMEKFIHKPSLKATMGLAGKARVAEKFSADAFADQLYQYVTKLV

Target ORF information:

RefSeq Version	NM_019998.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase) (Alg2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019998.3

ORF Insert Sequence:

ATGGCCGAAA ACCTGTACCG AGCCCGTTCC CGGGTTTACA GCCCATCTGT GCTGTTTCTG 
CACCCAGACA TGGGTATAGG CGGAGCCGAG CGCCTAGTGC TGGACGCGGC GCTGGCGCTG 
CAGGAGTACG GCTGTGATGT GAAGATATGG ACCGCGCACT ACGACCCGAA CCACTGCTTC 
ATCGAGACCC GCGAGCTCTC GGTGCAATGC GCAGGGGACT GGCTGCCTCG CAGCCTGGGC 
TGGGGCGGCC GCGGCGCCGC CATCTGCTCC TACGTGCGCA TGGTCTTTCT GGCGCTCTAC 
GTGCTGTTTC TCTCCGGCGA GGAGTTCGAC GTGGTGGTGT GCGACCAGGT GTCTGCCTGT 
ATCCCGGTGT TCAAACTGGC CAGACGGCGT AAGAGGGTCC TATTTTACTG TCACTTCCCA 
GATCTGCTGC TTACTCAGAG AAATTCAGCT CTGAAGAAGT TCTACAGGGC CCCCATCGAC 
TGGATCGAGG AATACACCAC AGGCATGGCA GACCGCATCT TGGTCAACAG CCAGTACACT 
GCTTCCGTCT TTAAAGAAAC CTTCAAGACC CTGTCTCACA GAAATCCTGA TGTGCTCTAC 
CCATCTCTGA ATATCGGCAG CTTTGACTTG GCTATTCCTG AAAAGATAGA TGACCTCGTC 
CCCAAGGGGA AGCAATTCCT GTTCCTCTCT ATCAACCGAT ACGAAAGGAA GAAAAATCTG 
CCCTTGGCAC TGAGATCCTT GGTGCAGCTT CGGAATCGGT TACCATCTCA AGAGTGGGAT 
AAGGTTCATC TCTTCATGGC CGGTGGTTAT GACGATAGGA TCCCGGAGAA CGTGGAGCAC 
TATAAGGAGT TGAAGAAAAT GGTCCAAGAG TCAGACCTTG AGCGTCATGT GACCTTCCTG 
CGGTCCTTCT CGGACAGACA GAAGATCTCA CTCCTCCACG GCTGCTTGTG TGTGCTCTAC 
ACTCCGAGCA ACGAGCACTT TGGCATCGTC CCTCTGGAGG CCATGTACAT GCAGTGCCCA 
GTCATCGCTG TTAATAACGG TGGGCCCCTG GAGTCCATTG TCCACAAGGT CACGGGGTTC 
CTGTGTGAGC CAGACCCAGT GCACTTCTCA GAAGCCATGG AGAAGTTCAT CCACAAACCA 
TCCTTAAAAG CCACGATGGG CCTCGCTGGA AAAGCCAGGG TGGCGGAGAA GTTTTCAGCT 
GATGCATTTG CAGACCAGCT GTACCAGTAT GTCACGAAGC TGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

ALG-2 divalent-ion affinity: Calorimetric analysis of the des23 versions reveals high-affinity site for Mg(2).
Biophysical chemistry20928-40(2016 Feb)
Henzl MT,Frey BB,Wolf AJ

Human immunodeficiency virus type 1 enhancer-binding protein 3 is essential for the expression of asparagine-linked glycosylation 2 in the regulation of osteoblast and chondrocyte differentiation.
The Journal of biological chemistry289(14)9865-79(2014 Apr)
Imamura K,Maeda S,Kawamura I,Matsuyama K,Shinohara N,Yahiro Y,Nagano S,Setoguchi T,Yokouchi M,Ishidou Y,Komiya S

A mouse knockout library for secreted and transmembrane proteins.
Nature biotechnology28(7)749-55(2010 Jul)
Tang T,Li L,Tang J,Li Y,Lin WY,Martin F,Grant D,Solloway M,Parker L,Ye W,Forrest W,Ghilardi N,Oravecz T,Platt KA,Rice DS,Hansen GM,Abuin A,Eberhart DE,Godowski P,Holt KH,Peterson A,Zambrowicz BP,de Sauvage FJ

The calcium binding protein ALG-2 binds and stabilizes Scotin, a p53-inducible gene product localized at the endoplasmic reticulum membrane.
Archives of biochemistry and biophysics467(1)87-94(2007 Nov)
Draeby I,Woods YL,la Cour JM,Mollerup J,Bourdon JC,Berchtold MW

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Alg2 cDNA ORF clone, Mus musculus(house mouse)

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