Cyp39a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp39a1
Entrez Gene ID	56050
Full Name	cytochrome P450, family 39, subfamily a, polypeptide 1
Synonyms	mCYP39A1
General protein information	Preferred Names cytochrome P450, family 39, subfamily a, polypeptide 1 Names 24-hydroxycholesterol 7-alpha-hydroxylase cytochrome P450, 39a1 (oxysterol 7alpha-hydroxylase) oxysterol 7-alpha-hydroxylase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001285947.1	NP_001272876.1	24-hydroxycholesterol 7-alpha-hydroxylase isoform 2
NM_001285948.1	NP_001272877.1	24-hydroxycholesterol 7-alpha-hydroxylase isoform 3
NM_018887.4	NP_061375.1	24-hydroxycholesterol 7-alpha-hydroxylase isoform 1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	CYP39A1	XP_001102611.1
Mus musculus (house mouse)	Cyp39a1	NP_061375.1
Magnaporthe oryzae (rice blast fungus)	MGG_07210	XP_003715422.1
Pan troglodytes (chimpanzee)	CYP39A1	XP_518518.2
Canis lupus familiaris (dog)	CYP39A1	XP_538946.2
Rattus norvegicus (Norway rat)	Cyp39a1	NP_001100363.1
Gallus gallus (chicken)	CYP39A1	XP_420063.3
Xenopus tropicalis (tropical clawed frog)	LOC100486848	XP_004918238.1
Homo sapiens (human)	CYP39A1	NP_057677.2
Bos taurus (cattle)	CYP39A1	NP_001092408.1
Danio rerio (zebrafish)	cyp39a1	NP_001025360.1

Gene Ontology
Bibliography

Related articles in PubMed

Zinc Fingers and Homeoboxes 2 (Zhx2) Regulates Sexually Dimorphic Cyp Gene Expression in the Adult Mouse Liver.
Creasy KT, Jiang J, Ren H, Peterson ML, Spear BT
Gene expression17(1)7-17(2016)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Gineste C, De Winter JM, Kohl C, Witt CC, Giannesini B, Brohm K, Le Fur Y, Gretz N, Vilmen C, Pecchi E, Jubeau M, Cozzone PJ, Stienen GJ, Granzier H, Labeit S, Ottenheijm CA, Bendahan D, Gondin J
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)

Cell-type-specific consequences of Reelin deficiency in the mouse neocortex, hippocampus, and amygdala.
Boyle MP, Bernard A, Thompson CL, Ng L, Boe A, Mortrud M, Hawrylycz MJ, Jones AR, Hevner RF, Lein ES
The Journal of comparative neurology519(11)2061-89(2011 Aug)

Comparative expression profiling of 40 mouse cytochrome P450 genes in embryonic and adult tissues.
Choudhary D, Jansson I, Schenkman JB, Sarfarazi M, Stoilov I
Archives of biochemistry and biophysics414(1)91-100(2003 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cyp39a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp39a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09042	NM_001285947.1 Latest version!	Mus musculus cytochrome P450, family 39, subfamily a, polypeptide 1 (Cyp39a1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu09045	NM_001285948.1 Latest version!	Mus musculus cytochrome P450, family 39, subfamily a, polypeptide 1 (Cyp39a1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu11136	NM_018887.4 Latest version!	Mus musculus cytochrome P450, family 39, subfamily a, polypeptide 1 (Cyp39a1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp39a1 ( NM_001285947.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001272876.1 Mus musculus cytochrome P450, family 39, subfamily a, polypeptide 1 (Cyp39a1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu09042
Clone ID Related Accession (Same CDS sequence)	NM_001285947.1
Accession Version	NM_001285947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571846400000
Organism	Mus musculus(house mouse)
Product	24-hydroxycholesterol 7-alpha-hydroxylase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE648427.1, AC154647.2, AC154204.2 and AK031733.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.37892.1, SRR1660811.245940.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGGCATTA TGGAGTTATT CTCACCAATA GCAATCGCCG TTCTGGGAAG CTGTGTTCTT 
TTCTTATTCT CTCGGTTGAA GAATTTGCTT GGACCCCCGT GCATCCAAGG CTGGATTCCC 
TGGATTGGAG CTGGACTTGA GTTTGGGAAA GCCCCTCTTG AATTTATAGA AAAAGCAAGA 
ATCAAGTATG GACCGGTATT TACCATCTTT GCTATGGGAA ACCGAATGAC CTTTGTTAGT 
GAAGAAGAAG GAATCAATGT GCTTCTAAAA TCTGAACATG TAGATTTTGA ATCAGCAGTA 
CAAAGTCCTG TCTATCACAC AGCATGGATT CCAAAGAATG TCTTCTCTGC ACTGCATGAG 
CGACTGTATG CCTTGATGAA AGGGAAGATG GGAACTTTCA ACACACATCA CTTTACTGGG 
CCGCTAACTG AAGAACTTCA TGAGCAACTG GAAGGTTTAG GCACTCATGG AACAATGGAC 
CTGAATGACT TTGTAAGAAA CTGGTCAAAA TCCAAAAGAT GGCTCCTGGC GCTGTTTGAG 
AAAAATATTG GCAACATAAA AGCTCACGGA TCTGCAGGTC ATTCTGGAAC CCTCTTGCAG 
GCCATATTGG AAGTGGTGGA GACGGAAACA CGTCAATACA GTCCAAATTA TGGACTGGTC 
GTACTTTGGG CTGCTCTGGC CAATGCTCCT CCTATTGCAT TCTGGACACT TGGATATATC 
CTGTCTCATC CCGATATCCA CAGGACTGTT TTAGAAAGCA TATCTTCTGT GTTTGGCACT 
GCAGGTAAAG ACAAGATTAA AGTGTCTGAA GATGACCTGA AGAAGCTTCT AATAATTAAG 
TGGTGCATTC TGGAATCTGT CCGCTTGAGA GCCCCTGGTG TCATTACTAG AAAGGTAGTG 
AAGCCAGTTA AAATTCTGAA TCACACAGTT CCTTCTGGTG ATCTGCTGAT GTTGTCTCCA 
TTCTGGCTAC ACAGAAATCC AAAATATTTT CCTGAACCTG AGTCATTCAA ACCTGAACGC 
TGGAAAGAGG CAAATTTAGA TAAGTACATT TTCTTGGACT ACTTCATGGC ATTTGGAGGC 
GGGAAGTTCC AGTGTCCTGG AAGGTGGTTT GCTCTCTTAG AGATTCAACT ATGCATTATC 
TTAGTGCTGT ATAAGTACGA ATGCAGTCTT CTGGACCCAT TACCAAAGCA GAGTTCTCGT 
CATTTGGTGG GTGTCCCCCA GCCTGCAGGG AAATGCCGGA TTGAATATAA ACAAAGAGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272876.1
CDS	238..1500
Translation	MGIMELFSPIAIAVLGSCVLFLFSRLKNLLGPPCIQGWIPWIGAGLEFGKAPLEFIEKARIKYGPVFTIFAMGNRMTFVSEEEGINVLLKSEHVDFESAVQSPVYHTAWIPKNVFSALHERLYALMKGKMGTFNTHHFTGPLTEELHEQLEGLGTHGTMDLNDFVRNWSKSKRWLLALFEKNIGNIKAHGSAGHSGTLLQAILEVVETETRQYSPNYGLVVLWAALANAPPIAFWTLGYILSHPDIHRTVLESISSVFGTAGKDKIKVSEDDLKKLLIIKWCILESVRLRAPGVITRKVVKPVKILNHTVPSGDLLMLSPFWLHRNPKYFPEPESFKPERWKEANLDKYIFLDYFMAFGGGKFQCPGRWFALLEIQLCIILVLYKYECSLLDPLPKQSSRHLVGVPQPAGKCRIEYKQRA

Target ORF information:

RefSeq Version	NM_001285947.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome P450, family 39, subfamily a, polypeptide 1 (Cyp39a1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001285947.1

ORF Insert Sequence:

ATGGGCATTA TGGAGTTATT CTCACCAATA GCAATCGCCG TTCTGGGAAG CTGTGTTCTT 
TTCTTATTCT CTCGGTTGAA GAATTTGCTT GGACCCCCGT GCATCCAAGG CTGGATTCCC 
TGGATTGGAG CTGGACTTGA GTTTGGGAAA GCCCCTCTTG AATTTATAGA AAAAGCAAGA 
ATCAAGTATG GACCGGTATT TACCATCTTT GCTATGGGAA ACCGAATGAC CTTTGTTAGT 
GAAGAAGAAG GAATCAATGT GCTTCTAAAA TCTGAACATG TAGATTTTGA ATCAGCAGTA 
CAAAGTCCTG TCTATCACAC AGCATGGATT CCAAAGAATG TCTTCTCTGC ACTGCATGAG 
CGACTGTATG CCTTGATGAA AGGGAAGATG GGAACTTTCA ACACACATCA CTTTACTGGG 
CCGCTAACTG AAGAACTTCA TGAGCAACTG GAAGGTTTAG GCACTCATGG AACAATGGAC 
CTGAATGACT TTGTAAGAAA CTGGTCAAAA TCCAAAAGAT GGCTCCTGGC GCTGTTTGAG 
AAAAATATTG GCAACATAAA AGCTCACGGA TCTGCAGGTC ATTCTGGAAC CCTCTTGCAG 
GCCATATTGG AAGTGGTGGA GACGGAAACA CGTCAATACA GTCCAAATTA TGGACTGGTC 
GTACTTTGGG CTGCTCTGGC CAATGCTCCT CCTATTGCAT TCTGGACACT TGGATATATC 
CTGTCTCATC CCGATATCCA CAGGACTGTT TTAGAAAGCA TATCTTCTGT GTTTGGCACT 
GCAGGTAAAG ACAAGATTAA AGTGTCTGAA GATGACCTGA AGAAGCTTCT AATAATTAAG 
TGGTGCATTC TGGAATCTGT CCGCTTGAGA GCCCCTGGTG TCATTACTAG AAAGGTAGTG 
AAGCCAGTTA AAATTCTGAA TCACACAGTT CCTTCTGGTG ATCTGCTGAT GTTGTCTCCA 
TTCTGGCTAC ACAGAAATCC AAAATATTTT CCTGAACCTG AGTCATTCAA ACCTGAACGC 
TGGAAAGAGG CAAATTTAGA TAAGTACATT TTCTTGGACT ACTTCATGGC ATTTGGAGGC 
GGGAAGTTCC AGTGTCCTGG AAGGTGGTTT GCTCTCTTAG AGATTCAACT ATGCATTATC 
TTAGTGCTGT ATAAGTACGA ATGCAGTCTT CTGGACCCAT TACCAAAGCA GAGTTCTCGT 
CATTTGGTGG GTGTCCCCCA GCCTGCAGGG AAATGCCGGA TTGAATATAA ACAAAGAGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyp39a1 ( NM_001285948.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001272877.1 Mus musculus cytochrome P450, family 39, subfamily a, polypeptide 1 (Cyp39a1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu09045
Clone ID Related Accession (Same CDS sequence)	NM_001285948.1
Accession Version	NM_001285948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571846400000
Organism	Mus musculus(house mouse)
Product	24-hydroxycholesterol 7-alpha-hydroxylase isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE648427.1, AC154647.2, AC154204.2 and AK031733.1. Transcript Variant: This variant (3) lacks an exon in the 3' coding region resulting in a frameshift compared to variant 1. The encoded protein (isoform 3) has a distinct C-terminus and is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC021418.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849375, SAMN00849380 [ECO:0000350] ##Evidence-Data-END##

ATGGGCATTA TGGAGTTATT CTCACCAATA GCAATCGCCG TTCTGGGAAG CTGTGTTCTT 
TTCTTATTCT CTCGGTTGAA GAATTTGCTT GGACCCCCGT GCATCCAAGG CTGGATTCCC 
TGGATTGGAG CTGGACTTGA GTTTGGGAAA GCCCCTCTTG AATTTATAGA AAAAGCAAGA 
ATCAAGTATG GACCGGTATT TACCATCTTT GCTATGGGAA ACCGAATGAC CTTTGTTAGT 
GAAGAAGAAG GAATCAATGT GCTTCTAAAA TCTGAACATG TAGATTTTGA ATCAGCAGTA 
CAAAGTCCTG TCTATCACAC AGCATGGATT CCAAAGAATG TCTTCTCTGC ACTGCATGAG 
CGACTGTATG CCTTGATGAA AGGGAAGATG GGAACTTTCA ACACACATCA CTTTACTGGG 
CCGCTAACTG AAGAACTTCA TGAGCAACTG GAAGGTTTAG GCACTCATGG AACAATGGAC 
CTGAATGACT TTGTAAGGTA TCTACTTTAT CCAGCCACAC TCAATACCCT CTTTAAGAAA 
GGGTTGTTTC TCACAGACAA GAGGACAATC AAGGAATTCT ACCAGCAGTT TAAAACCTAT 
GATGAGGGCT TTGAGTATGG CTCCCAGCTG CCTGAATGGC TTCTGAGAAA CTGGTCAAAA 
TCCAAAAGAT GGCTCCTGGC GCTGTTTGAG AAAAATATTG GCAACATAAA AGCTCACGGA 
TCTGCAGGTC ATTCTGGAAC CCTCTTGCAG GCCATATTGG AAGTGGTGGA GACGGAAACA 
CGTCAATACA GTCCAAATTA TGGACTGGTC GTACTTTGGG CTGCTCTGGC CAATGCTCCT 
CCTATTGCAT TCTGGACACT TGGATATATC CTGTCTCATC CCGATATCCA CAGGACTGTT 
TTAGAAAGCA TATCTTCTGT GTTTGGCACT GCAGGTAAAG ACAAGATTAA AGTGTCTGAA 
GATGACCTGA AGAAGCTTCT AATAATTAAG TGGTGCATTC TGGAATCTGT CCGCTTGAGA 
GCCCCTGGTG TCATTACTAG AAAGGTAGTG AAGCCAGTTA AAATTCTGAA TCACACAGTT 
CCTTCTGGTG ATCTGCTGAT GTTGTCTCCA TTCTGGCTAC ACAGAAATCC AAAATATTTT 
CCTGAACCTG AGTCATTCAA ACCTGTGGTT TGCTCTCTTA GAGATTCAAC TATGCATTAT 
CTTAGTGCTG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272877.1
CDS	238..1452
Translation	MGIMELFSPIAIAVLGSCVLFLFSRLKNLLGPPCIQGWIPWIGAGLEFGKAPLEFIEKARIKYGPVFTIFAMGNRMTFVSEEEGINVLLKSEHVDFESAVQSPVYHTAWIPKNVFSALHERLYALMKGKMGTFNTHHFTGPLTEELHEQLEGLGTHGTMDLNDFVRYLLYPATLNTLFKKGLFLTDKRTIKEFYQQFKTYDEGFEYGSQLPEWLLRNWSKSKRWLLALFEKNIGNIKAHGSAGHSGTLLQAILEVVETETRQYSPNYGLVVLWAALANAPPIAFWTLGYILSHPDIHRTVLESISSVFGTAGKDKIKVSEDDLKKLLIIKWCILESVRLRAPGVITRKVVKPVKILNHTVPSGDLLMLSPFWLHRNPKYFPEPESFKPVVCSLRDSTMHYLSAV

Target ORF information:

RefSeq Version	NM_001285948.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome P450, family 39, subfamily a, polypeptide 1 (Cyp39a1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001285948.1

ORF Insert Sequence:

ATGGGCATTA TGGAGTTATT CTCACCAATA GCAATCGCCG TTCTGGGAAG CTGTGTTCTT 
TTCTTATTCT CTCGGTTGAA GAATTTGCTT GGACCCCCGT GCATCCAAGG CTGGATTCCC 
TGGATTGGAG CTGGACTTGA GTTTGGGAAA GCCCCTCTTG AATTTATAGA AAAAGCAAGA 
ATCAAGTATG GACCGGTATT TACCATCTTT GCTATGGGAA ACCGAATGAC CTTTGTTAGT 
GAAGAAGAAG GAATCAATGT GCTTCTAAAA TCTGAACATG TAGATTTTGA ATCAGCAGTA 
CAAAGTCCTG TCTATCACAC AGCATGGATT CCAAAGAATG TCTTCTCTGC ACTGCATGAG 
CGACTGTATG CCTTGATGAA AGGGAAGATG GGAACTTTCA ACACACATCA CTTTACTGGG 
CCGCTAACTG AAGAACTTCA TGAGCAACTG GAAGGTTTAG GCACTCATGG AACAATGGAC 
CTGAATGACT TTGTAAGGTA TCTACTTTAT CCAGCCACAC TCAATACCCT CTTTAAGAAA 
GGGTTGTTTC TCACAGACAA GAGGACAATC AAGGAATTCT ACCAGCAGTT TAAAACCTAT 
GATGAGGGCT TTGAGTATGG CTCCCAGCTG CCTGAATGGC TTCTGAGAAA CTGGTCAAAA 
TCCAAAAGAT GGCTCCTGGC GCTGTTTGAG AAAAATATTG GCAACATAAA AGCTCACGGA 
TCTGCAGGTC ATTCTGGAAC CCTCTTGCAG GCCATATTGG AAGTGGTGGA GACGGAAACA 
CGTCAATACA GTCCAAATTA TGGACTGGTC GTACTTTGGG CTGCTCTGGC CAATGCTCCT 
CCTATTGCAT TCTGGACACT TGGATATATC CTGTCTCATC CCGATATCCA CAGGACTGTT 
TTAGAAAGCA TATCTTCTGT GTTTGGCACT GCAGGTAAAG ACAAGATTAA AGTGTCTGAA 
GATGACCTGA AGAAGCTTCT AATAATTAAG TGGTGCATTC TGGAATCTGT CCGCTTGAGA 
GCCCCTGGTG TCATTACTAG AAAGGTAGTG AAGCCAGTTA AAATTCTGAA TCACACAGTT 
CCTTCTGGTG ATCTGCTGAT GTTGTCTCCA TTCTGGCTAC ACAGAAATCC AAAATATTTT 
CCTGAACCTG AGTCATTCAA ACCTGTGGTT TGCTCTCTTA GAGATTCAAC TATGCATTAT 
CTTAGTGCTG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyp39a1 ( NM_018887.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061375.1 Mus musculus cytochrome P450, family 39, subfamily a, polypeptide 1 (Cyp39a1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11136
Clone ID Related Accession (Same CDS sequence)	NM_018887.4
Accession Version	NM_018887.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Mus musculus(house mouse)
Product	24-hydroxycholesterol 7-alpha-hydroxylase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE648427.1, AC154647.2 and AK031733.1. On Oct 25, 2013 this sequence version replaced NM_018887.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK031733.1, SRR1660813.120587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGGCATTA TGGAGTTATT CTCACCAATA GCAATCGCCG TTCTGGGAAG CTGTGTTCTT 
TTCTTATTCT CTCGGTTGAA GAATTTGCTT GGACCCCCGT GCATCCAAGG CTGGATTCCC 
TGGATTGGAG CTGGACTTGA GTTTGGGAAA GCCCCTCTTG AATTTATAGA AAAAGCAAGA 
ATCAAGTATG GACCGGTATT TACCATCTTT GCTATGGGAA ACCGAATGAC CTTTGTTAGT 
GAAGAAGAAG GAATCAATGT GCTTCTAAAA TCTGAACATG TAGATTTTGA ATCAGCAGTA 
CAAAGTCCTG TCTATCACAC AGCATGGATT CCAAAGAATG TCTTCTCTGC ACTGCATGAG 
CGACTGTATG CCTTGATGAA AGGGAAGATG GGAACTTTCA ACACACATCA CTTTACTGGG 
CCGCTAACTG AAGAACTTCA TGAGCAACTG GAAGGTTTAG GCACTCATGG AACAATGGAC 
CTGAATGACT TTGTAAGGTA TCTACTTTAT CCAGCCACAC TCAATACCCT CTTTAAGAAA 
GGGTTGTTTC TCACAGACAA GAGGACAATC AAGGAATTCT ACCAGCAGTT TAAAACCTAT 
GATGAGGGCT TTGAGTATGG CTCCCAGCTG CCTGAATGGC TTCTGAGAAA CTGGTCAAAA 
TCCAAAAGAT GGCTCCTGGC GCTGTTTGAG AAAAATATTG GCAACATAAA AGCTCACGGA 
TCTGCAGGTC ATTCTGGAAC CCTCTTGCAG GCCATATTGG AAGTGGTGGA GACGGAAACA 
CGTCAATACA GTCCAAATTA TGGACTGGTC GTACTTTGGG CTGCTCTGGC CAATGCTCCT 
CCTATTGCAT TCTGGACACT TGGATATATC CTGTCTCATC CCGATATCCA CAGGACTGTT 
TTAGAAAGCA TATCTTCTGT GTTTGGCACT GCAGGTAAAG ACAAGATTAA AGTGTCTGAA 
GATGACCTGA AGAAGCTTCT AATAATTAAG TGGTGCATTC TGGAATCTGT CCGCTTGAGA 
GCCCCTGGTG TCATTACTAG AAAGGTAGTG AAGCCAGTTA AAATTCTGAA TCACACAGTT 
CCTTCTGGTG ATCTGCTGAT GTTGTCTCCA TTCTGGCTAC ACAGAAATCC AAAATATTTT 
CCTGAACCTG AGTCATTCAA ACCTGAACGC TGGAAAGAGG CAAATTTAGA TAAGTACATT 
TTCTTGGACT ACTTCATGGC ATTTGGAGGC GGGAAGTTCC AGTGTCCTGG AAGGTGGTTT 
GCTCTCTTAG AGATTCAACT ATGCATTATC TTAGTGCTGT ATAAGTACGA ATGCAGTCTT 
CTGGACCCAT TACCAAAGCA GAGTTCTCGT CATTTGGTGG GTGTCCCCCA GCCTGCAGGG 
AAATGCCGGA TTGAATATAA ACAAAGAGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061375.1
CDS	238..1650
Translation	MGIMELFSPIAIAVLGSCVLFLFSRLKNLLGPPCIQGWIPWIGAGLEFGKAPLEFIEKARIKYGPVFTIFAMGNRMTFVSEEEGINVLLKSEHVDFESAVQSPVYHTAWIPKNVFSALHERLYALMKGKMGTFNTHHFTGPLTEELHEQLEGLGTHGTMDLNDFVRYLLYPATLNTLFKKGLFLTDKRTIKEFYQQFKTYDEGFEYGSQLPEWLLRNWSKSKRWLLALFEKNIGNIKAHGSAGHSGTLLQAILEVVETETRQYSPNYGLVVLWAALANAPPIAFWTLGYILSHPDIHRTVLESISSVFGTAGKDKIKVSEDDLKKLLIIKWCILESVRLRAPGVITRKVVKPVKILNHTVPSGDLLMLSPFWLHRNPKYFPEPESFKPERWKEANLDKYIFLDYFMAFGGGKFQCPGRWFALLEIQLCIILVLYKYECSLLDPLPKQSSRHLVGVPQPAGKCRIEYKQRA

Target ORF information:

RefSeq Version	NM_018887.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome P450, family 39, subfamily a, polypeptide 1 (Cyp39a1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018887.4

ORF Insert Sequence:

ATGGGCATTA TGGAGTTATT CTCACCAATA GCAATCGCCG TTCTGGGAAG CTGTGTTCTT 
TTCTTATTCT CTCGGTTGAA GAATTTGCTT GGACCCCCGT GCATCCAAGG CTGGATTCCC 
TGGATTGGAG CTGGACTTGA GTTTGGGAAA GCCCCTCTTG AATTTATAGA AAAAGCAAGA 
ATCAAGTATG GACCGGTATT TACCATCTTT GCTATGGGAA ACCGAATGAC CTTTGTTAGT 
GAAGAAGAAG GAATCAATGT GCTTCTAAAA TCTGAACATG TAGATTTTGA ATCAGCAGTA 
CAAAGTCCTG TCTATCACAC AGCATGGATT CCAAAGAATG TCTTCTCTGC ACTGCATGAG 
CGACTGTATG CCTTGATGAA AGGGAAGATG GGAACTTTCA ACACACATCA CTTTACTGGG 
CCGCTAACTG AAGAACTTCA TGAGCAACTG GAAGGTTTAG GCACTCATGG AACAATGGAC 
CTGAATGACT TTGTAAGGTA TCTACTTTAT CCAGCCACAC TCAATACCCT CTTTAAGAAA 
GGGTTGTTTC TCACAGACAA GAGGACAATC AAGGAATTCT ACCAGCAGTT TAAAACCTAT 
GATGAGGGCT TTGAGTATGG CTCCCAGCTG CCTGAATGGC TTCTGAGAAA CTGGTCAAAA 
TCCAAAAGAT GGCTCCTGGC GCTGTTTGAG AAAAATATTG GCAACATAAA AGCTCACGGA 
TCTGCAGGTC ATTCTGGAAC CCTCTTGCAG GCCATATTGG AAGTGGTGGA GACGGAAACA 
CGTCAATACA GTCCAAATTA TGGACTGGTC GTACTTTGGG CTGCTCTGGC CAATGCTCCT 
CCTATTGCAT TCTGGACACT TGGATATATC CTGTCTCATC CCGATATCCA CAGGACTGTT 
TTAGAAAGCA TATCTTCTGT GTTTGGCACT GCAGGTAAAG ACAAGATTAA AGTGTCTGAA 
GATGACCTGA AGAAGCTTCT AATAATTAAG TGGTGCATTC TGGAATCTGT CCGCTTGAGA 
GCCCCTGGTG TCATTACTAG AAAGGTAGTG AAGCCAGTTA AAATTCTGAA TCACACAGTT 
CCTTCTGGTG ATCTGCTGAT GTTGTCTCCA TTCTGGCTAC ACAGAAATCC AAAATATTTT 
CCTGAACCTG AGTCATTCAA ACCTGAACGC TGGAAAGAGG CAAATTTAGA TAAGTACATT 
TTCTTGGACT ACTTCATGGC ATTTGGAGGC GGGAAGTTCC AGTGTCCTGG AAGGTGGTTT 
GCTCTCTTAG AGATTCAACT ATGCATTATC TTAGTGCTGT ATAAGTACGA ATGCAGTCTT 
CTGGACCCAT TACCAAAGCA GAGTTCTCGT CATTTGGTGG GTGTCCCCCA GCCTGCAGGG 
AAATGCCGGA TTGAATATAA ACAAAGAGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zinc Fingers and Homeoboxes 2 (Zhx2) Regulates Sexually Dimorphic Cyp Gene Expression in the Adult Mouse Liver.
Gene expression17(1)7-17(2016)
Creasy KT,Jiang J,Ren H,Peterson ML,Spear BT

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)
Gineste C,De Winter JM,Kohl C,Witt CC,Giannesini B,Brohm K,Le Fur Y,Gretz N,Vilmen C,Pecchi E,Jubeau M,Cozzone PJ,Stienen GJ,Granzier H,Labeit S,Ottenheijm CA,Bendahan D,Gondin J

Cell-type-specific consequences of Reelin deficiency in the mouse neocortex, hippocampus, and amygdala.
The Journal of comparative neurology519(11)2061-89(2011 Aug)
Boyle MP,Bernard A,Thompson CL,Ng L,Boe A,Mortrud M,Hawrylycz MJ,Jones AR,Hevner RF,Lein ES

Comparative expression profiling of 40 mouse cytochrome P450 genes in embryonic and adult tissues.
Archives of biochemistry and biophysics414(1)91-100(2003 Jun)
Choudhary D,Jansson I,Schenkman JB,Sarfarazi M,Stoilov I

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Cyp39a1 cDNA ORF clone, Mus musculus(house mouse)

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