Rnf5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnf5
Entrez Gene ID	54197
Full Name	ring finger protein 5
Synonyms	2410131O05Rik,AA407576,NG2
General protein information	Preferred Names ring finger protein 5 Names E3 ubiquitin-protein ligase RNF5 RING-type E3 ubiquitin transferase RNF5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B1\|17 18.18 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_019403.3	NP_062276.1	E3 ubiquitin-protein ligase RNF5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RNF5	XP_001164301.1
Canis lupus familiaris (dog)	RNF5	XP_532092.1
Homo sapiens (human)	RNF5	NP_008844.1
Xenopus tropicalis (tropical clawed frog)	rnf5	NP_001017071.1
Bos taurus (cattle)	RNF5	NP_001098915.1
Rattus norvegicus (Norway rat)	Rnf5	NP_001102495.1
Macaca mulatta (Rhesus monkey)	RNF5	XP_001114771.1
Mus musculus (house mouse)	Rnf5	NP_062276.1
Danio rerio (zebrafish)	LOC100537822	XP_003200552.1

Gene Ontology
Bibliography

Related articles in PubMed

Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5
Li Y, Tinoco R, Elmén L, Segota I, Xian Y, Fujita Y, Sahu A, Zarecki R, Marie K, Feng Y, Khateb A, Frederick DT, Ashkenazi SK, Kim H, Perez EG, Day CP, Segura Muñoz RS, Schmaltz R, Yooseph S, Tam MA, Zhang T, Avitan-Hersh E, Tzur L, Roizman S, Boyango I, Bar-Sela G, Orian A, Kaufman RJ, Bosenberg M, Goding CR, Baaten B, Levesque MP, Dummer R, Brown K, Merlino G, Ruppin E, Flaherty K, Ramer-Tait A, Long T, Peterson SN, Bradley LM, Ronai ZA
Nature communications10(1)1492(2019 04)

Regulation of S100A8 Stability by RNF5 in Intestinal Epithelial Cells Determines Intestinal Inflammation and Severity of Colitis.
Fujita Y, Khateb A, Li Y, Tinoco R, Zhang T, Bar-Yoseph H, Tam MA, Chowers Y, Sabo E, Gerassy-Vainberg S, Starosvetsky E, James B, Brown K, Shen-Orr SS, Bradley LM, Tessier PA, Ronai ZA
Cell reports24(12)3296-3311.e6(2018 09)

Genetic Inhibition Of The Ubiquitin Ligase Rnf5 Attenuates Phenotypes Associated To F508del Cystic Fibrosis Mutation.
Tomati V, Sondo E, Armirotti A, Caci E, Pesce E, Marini M, Gianotti A, Jeon YJ, Cilli M, Pistorio A, Mastracci L, Ravazzolo R, Scholte B, Ronai Z, Galietta LJ, Pedemonte N
Scientific reports512138(2015 Jul)

Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies.
Jeon YJ, Khelifa S, Ratnikov B, Scott DA, Feng Y, Parisi F, Ruller C, Lau E, Kim H, Brill LM, Jiang T, Rimm DL, Cardiff RD, Mills GB, Smith JW, Osterman AL, Kluger Y, Ronai ZA
Cancer cell27(3)354-69(2015 Mar)

Regulation of ATG4B stability by RNF5 limits basal levels of autophagy and influences susceptibility to bacterial infection.
Kuang E, Okumura CY, Sheffy-Levin S, Varsano T, Shu VC, Qi J, Niesman IR, Yang HJ, López-Otín C, Yang WY, Reed JC, Broday L, Nizet V, Ronai ZA
PLoS genetics8(10)e1003007(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rnf5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnf5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10820	NM_019403.3 Latest version!	Mus musculus ring finger protein 5 (Rnf5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnf5 ( NM_019403.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062276.1 Mus musculus ring finger protein 5 (Rnf5), mRNA.

Price & Availability↑

CloneID	OMu10820
Clone ID Related Accession (Same CDS sequence)	NM_019403.3
Accession Version	NM_019403.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CT009767.6. On Nov 17, 2006 this sequence version replaced NM_019403.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016449.1, AK019289.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139, SAMN01164140 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGCAG CGGAGGAAGA AGACGGGGGC CCCGAAGGGC CAAATCGCGA GCGGGGCGGG 
GCGAGCGCGA CCTTCGAATG TAATATATGT CTGGAGACAG CTCGCGAAGC TGTGGTCAGC 
GTGTGTGGCC ACCTGTATTG TTGGCCCTGT CTTCACCAGT GGCTGGAGAC CCGGCCAGAC 
CGGCAAGAAT GCCCGGTGTG TAAAGCTGGC ATCAGCAGGG AGAAGGTCGT CCCTCTTTAT 
GGGCGAGGGA GCCAGAAGCC ACAGGATCCC AGATTGAAAA CTCCACCCCG CCCTCAGGGC 
CAGCGACCAG CTCCAGAGAG CAGAGGGGGG TTCCAGCCAT TTGGTGATGC CGGGGGATTC 
CACTTCTCCT TTGGTGTCGG TGCCTTCCCC TTTGGCTTTT TCACCACCGT GTTCAATGCC 
CATGAACCTT TCAGAAGAGG CGCAGGTGTG GATCTGGGAC AGGGTCACCC AGCCTCCAGC 
TGGCAAGATT CCCTATTCCT GTTCCTCGCC ATCTTTTTCT TTTTCTGGCT GCTCAGTATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062276.1
CDS	89..631
Translation	MAAAEEEDGGPEGPNRERGGASATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPDRQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPESRGGFQPFGDAGGFHFSFGVGAFPFGFFTTVFNAHEPFRRGAGVDLGQGHPASSWQDSLFLFLAIFFFFWLLSI

Target ORF information:

RefSeq Version	NM_019403.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 5 (Rnf5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019403.3

ORF Insert Sequence:

ATGGCAGCAG CGGAGGAAGA AGACGGGGGC CCCGAAGGGC CAAATCGCGA GCGGGGCGGG 
GCGAGCGCGA CCTTCGAATG TAATATATGT CTGGAGACAG CTCGCGAAGC TGTGGTCAGC 
GTGTGTGGCC ACCTGTATTG TTGGCCCTGT CTTCACCAGT GGCTGGAGAC CCGGCCAGAC 
CGGCAAGAAT GCCCGGTGTG TAAAGCTGGC ATCAGCAGGG AGAAGGTCGT CCCTCTTTAT 
GGGCGAGGGA GCCAGAAGCC ACAGGATCCC AGATTGAAAA CTCCACCCCG CCCTCAGGGC 
CAGCGACCAG CTCCAGAGAG CAGAGGGGGG TTCCAGCCAT TTGGTGATGC CGGGGGATTC 
CACTTCTCCT TTGGTGTCGG TGCCTTCCCC TTTGGCTTTT TCACCACCGT GTTCAATGCC 
CATGAACCTT TCAGAAGAGG CGCAGGTGTG GATCTGGGAC AGGGTCACCC AGCCTCCAGC 
TGGCAAGATT CCCTATTCCT GTTCCTCGCC ATCTTTTTCT TTTTCTGGCT GCTCAGTATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5
Nature communications10(1)1492(2019 04)
Li Y,Tinoco R,Elmén L,Segota I,Xian Y,Fujita Y,Sahu A,Zarecki R,Marie K,Feng Y,Khateb A,Frederick DT,Ashkenazi SK,Kim H,Perez EG,Day CP,Segura Muñoz RS,Schmaltz R,Yooseph S,Tam MA,Zhang T,Avitan-Hersh E,Tzur L,Roizman S,Boyango I,Bar-Sela G,Orian A,Kaufman RJ,Bosenberg M,Goding CR,Baaten B,Levesque MP,Dummer R,Brown K,Merlino G,Ruppin E,Flaherty K,Ramer-Tait A,Long T,Peterson SN,Bradley LM,Ronai ZA

Regulation of S100A8 Stability by RNF5 in Intestinal Epithelial Cells Determines Intestinal Inflammation and Severity of Colitis.
Cell reports24(12)3296-3311.e6(2018 09)
Fujita Y,Khateb A,Li Y,Tinoco R,Zhang T,Bar-Yoseph H,Tam MA,Chowers Y,Sabo E,Gerassy-Vainberg S,Starosvetsky E,James B,Brown K,Shen-Orr SS,Bradley LM,Tessier PA,Ronai ZA

Genetic Inhibition Of The Ubiquitin Ligase Rnf5 Attenuates Phenotypes Associated To F508del Cystic Fibrosis Mutation.
Scientific reports512138(2015 Jul)
Tomati V,Sondo E,Armirotti A,Caci E,Pesce E,Marini M,Gianotti A,Jeon YJ,Cilli M,Pistorio A,Mastracci L,Ravazzolo R,Scholte B,Ronai Z,Galietta LJ,Pedemonte N

Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies.
Cancer cell27(3)354-69(2015 Mar)
Jeon YJ,Khelifa S,Ratnikov B,Scott DA,Feng Y,Parisi F,Ruller C,Lau E,Kim H,Brill LM,Jiang T,Rimm DL,Cardiff RD,Mills GB,Smith JW,Osterman AL,Kluger Y,Ronai ZA

Regulation of ATG4B stability by RNF5 limits basal levels of autophagy and influences susceptibility to bacterial infection.
PLoS genetics8(10)e1003007(2012)
Kuang E,Okumura CY,Sheffy-Levin S,Varsano T,Shu VC,Qi J,Niesman IR,Yang HJ,López-Otín C,Yang WY,Reed JC,Broday L,Nizet V,Ronai ZA

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Rnf5 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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