Pkn1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pkn1
Entrez Gene ID	320795
Full Name	protein kinase N1
Synonyms	DBK,F730027O18Rik,PAK1,PRK1,Pkn,Prkcl1,Stk3
General protein information	Preferred Names protein kinase N1 Names serine/threonine-protein kinase N1 protein kinase C-like 1 protein kinase C-like PKN protein-kinase C-related kinase 1 serine-threonine protein kinase N serine/threonine kinase 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 C2\|8 40.22 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_030243621.1	XP_030099481.1	serine/threonine-protein kinase N1 isoform X1
NM_177262.4	NP_796236.2	serine/threonine-protein kinase N1 isoform 2
NM_001199593.1	NP_001186522.1	serine/threonine-protein kinase N1 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PKN1	XP_512443.3
Danio rerio (zebrafish)	LOC560840	XP_005160108.1
Homo sapiens (human)	PKN1	NP_002732.3
Bos taurus (cattle)	PKN1	NP_001073715.1
Rattus norvegicus (Norway rat)	Pkn1	NP_058871.2
Canis lupus familiaris (dog)	PKN1	XP_003639827.1
Danio rerio (zebrafish)	zgc:162290	NP_001082929.1
Macaca mulatta (Rhesus monkey)	LOC718834	XP_002808221.1
Mus musculus (house mouse)	Pkn1	NP_796236.2

Gene Ontology
Bibliography

Related articles in PubMed

The homeoprotein Msx1 cooperates with Pkn1 to prevent terminal differentiation in myogenic precursor cells.
Zhu X, Li M, Jia X, Hou W, Yang J, Zhao H, Wang G, Wang J
Biochimie16255-65(2019 Jul)

Protein kinase N1 critically regulates cerebellar development and long-term function.
zur Nedden S, Eith R, Schwarzer C, Zanetti L, Seitter H, Fresser F, Koschak A, Cameron AJ, Parker PJ, Baier G, Baier-Bitterlich G
The Journal of clinical investigation128(5)2076-2088(2018 05)

Loss of Protein Kinase Novel 1 (PKN1) is associated with mild systolic and diastolic contractile dysfunction, increased phospholamban Thr17 phosphorylation, and exacerbated ischaemia-reperfusion injury.
Francois AA, Obasanjo-Blackshire K, Clark JE, Boguslavskyi A, Holt MR, Parker PJ, Marber MS, Heads RJ
Cardiovascular research114(1)138-157(2018 01)

The protein kinase C super-family member PKN is regulated by mTOR and influences differentiation during prostate cancer progression.
Yang CS, Melhuish TA, Spencer A, Ni L, Hao Y, Jividen K, Harris TE, Snow C, Frierson HF Jr, Wotton D, Paschal BM
The Prostate77(15)1452-1467(2017 Nov)

Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1.
Mashud R, Nomachi A, Hayakawa A, Kubouchi K, Danno S, Hirata T, Matsuo K, Nakayama T, Satoh R, Sugiura R, Abe M, Sakimura K, Wakana S, Ohsaki H, Kamoshida S, Mukai H
Scientific reports7(1)7663(2017 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pkn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pkn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02273	NM_001199593.1 Latest version!	Mus musculus protein kinase N1 (Pkn1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu02273	XM_030243621.1 Latest version!	Mus musculus protein kinase N1 (Pkn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu19239	NM_177262.4 Latest version!	Mus musculus protein kinase N1 (Pkn1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pkn1 ( NM_001199593.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001186522.1 Mus musculus protein kinase N1 (Pkn1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02273
Clone ID Related Accession (Same CDS sequence)	XM_030243621.1 , NM_001199593.1
Accession Version	NM_001199593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase N1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC164432.3, AK089431.1, AK149649.1 and CX568360.1. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK149649.1, AK150062.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGCTG ATCCCCCTTT GGATTCGGAG CTGGAGAGTG AGCCTCGAAG CTGGTCGCTG 
CTGGAACAGC TGGGTCTGGC TGGGGCTGAC CTGGCAGCCC CCGGGGTGCA GCAACAGCTG 
GAGTTAGAGC GAGAGCGACT GAAGCGGGAG ATACGGAAGG AGCTGAAGCT GAAGGAGGGC 
GCTGAGAACC TGAGGCGAGC CACCACTGAC CTGGGCCGTA GCCTGGCCCC TGTGGAGCTG 
CTGCTGCGGG GCTCCGCTCG ACGCCTAGAC TTACTGCACC AGCAGCTGCA GGAGCTGCAT 
GCACATGTGG TGCTGCCTGA CCCTGCAGCT GGAAGCGATG CTACCCAATC CCTTGCAGAG 
GGCAGCCCTA TCTGCTCATC CACCAACCTG AGCAGAGTGG CTGGGCTGGA GAAGCAGCTG 
GCCATTGAGC TCAAGGTCAA ACAGGGGGCA GAGAACATGA TCCAGACCTA TAGCAATGGC 
AGCAGCAAGG ACCGGAAGCT GTTGTTGACA GCCCAGCAGA TGCTGCAGGA CAGTAAGACC 
AAGATTGACA TCATCCGCAT GCAGCTTCGA AGAGCGCTCC AGGCACTACA GGCTGGAGAG 
CTGGAGAGTC AGGCAGCTCC TGATGAAGCC CAAGGAGATC CAGAACTGGG AGCCGTAGAG 
CTACGCATTG AGGAGCTACG ACACCATTTT CGAGTGGAGC ATGCAGTGGC AGAAGGTGCC 
AAGAATGTCC TGCGCCTGCT CAGTGGGGCA AAGGCCCCAG ACCGCAAGGC AGTCAGCGAG 
GCTCAGGAGA AATTGACTGA GTCCAACCAG AAGCTGGGCT TGCTGCGGGA ATCACTGGAG 
AGGCGCCTTG GGGAGTTGCC TGCCGATCAC CCCAAGGGGC GGCTGCTTCG GGAGGAGCTC 
ACTGCAGCCT CGTCCTCAGC CTTCAGCGCC ATACTGCCTG GGCCCTTCCC TGCCACTCAC 
TACAGCACCT TGAGCAAGCC TGCACCGCTC ACAGGGACCC TGGAAGTACG AGTGGTGGGC 
TGCAAAAACC TTCCCGAGAC CATCCCCTGG AGCCCTCCCC CCTCAGTGGG GGCATCTGGG 
ACCCCCGAAA GCCGCACTCC GTTCCTGAGT CGCCCCGCTC GGGGCCTTTA CAGCCGGAGT 
GGAAGCCTTA GTGGACGGAG CAGCCTCAGA GGGGAGGCAG AGAATGCCAC TGAGGTCAGC 
ACCGTGCTCA AGCTGGACAA CACAGTGGTG GGGCAAACAG CCTGGAAGCC ATGCGGCCCC 
AATGCCTGGG ACCAGAGCTT TACCCTGGAG CTGGAGAGGG CTCGGGAGCT GGAGCTGGCT 
GTGTTCTGGC GAGACCAGAG GGGTCTGTGT GCTCTCAAAT TTCTGAAGTT GGAGGACTTC 
TTGGACAACG AGAGGCATGA GGTGCAGCTG GACATGGAAC CCCAGGGCTG CCTGGTGGCT 
GAGGTCACTT TCCGAAACCC CATCATCGAG CGGATCCCTA GGCTCCAAAG GCAGAAAAAA 
ATCTTCTCCA AGCAGCAAGG GAAGGCATTT CAGCGAGCCA GACAGATGAA CATAGATGTG 
GCGACTTGGG TGCGGCTGCT CCGTAGACTC ATCCCTAGTG CTGTGGCCAC TGGCACCTTC 
AGTCCCAATG CATCTCCAGG TGCTGAGATC CGGCACACTG GAGACATATC CATGGAGAAA 
TTGAATCTCG GTGCTGACTC AGACAGCTCG TCCCAAAAGA GCCCACCAGG GCTGCCCTCC 
ACCTCATGCA GCCTGAGTTC TCCAACCCAT GAATCCACCA CATCTCCAGA GCTGCCTTCA 
GAGACCCAGG AGACTCCAGG CCCTGGCCTG TGCAGCCCCT TGAGAAAGTC GCCCCTGACA 
CTTGAGGACT TCAAGTTCCT GGCCGTGCTT GGCCGGGGTC ACTTTGGAAA GGTGCTGCTG 
TCTGAATTCC GCTCCAGTGG GGAGCTCTTT GCCATCAAAG CCTTGAAGAA AGGTGACATT 
GTAGCCCGAG ATGAGGTTGA GAGCCTGATG TGTGAGAAGC GGATTTTGGC GGCCGTGACC 
AGGGCAGGAC ATCCCTTCCT GGTGAACCTT TTCGGCTGTT TCCAGACCCC AGAGCACGTG 
TGCTTTGTGA TGGAGTACTC GGCGGGTGGA GACCTGATGC TGCACATTCA TAGCGACGTG 
TTCTCAGAGC CTCGGGCTGT CTTCTATTCG GCCTGTGTGG TGCTGGGACT GCAGTTCCTC 
CATGAACACA AGATTGTCTA CAGGGACCTG AAGTTGGACA ATTTGCTCCT GGATACTGAG 
GGCTACGTCA AGATCGCAGA CTTTGGCCTC TGCAAGGAGG GGATGGGCTA TGGGGACCGG 
ACCAGCACGT TCTGCGGAAC TCCGGAGTTC CTGGCGCCGG AAGTGCTCAC AGACACATCC 
TACACGCGAG CAGTGGACTG GTGGGGACTG GGCGTGCTGC TCTATGAGAT GTTGGTTGGA 
GAGTCTCCGT TCCCTGGGGA TGATGAGGAG GAGGTATTTG ACAGCATTGT CAACGACGAA 
GTTCGCTATC CCCGCTTCCT GTCTGCAGAG GCCATCGGCA TCATGAGAAG GCTACTGCGG 
AGGAACCCGG AGCGGAGGCT GGGGTCCACT GAGCGCGATG CAGAAGATGT GAAAAAACAG 
CCTTTCTTCC GGTCTCTGGG CTGGGATGTC CTGCTGGCCC GCCGCTTGCC TCCACCCTTC 
GTGCCTACAC TTTCAGGGCG CACAGATGTC AGCAACTTCG ATGAGGAGTT CACTGGGGAG 
GCCCCCACAC TGAGTCCTCC CCGGGATGCA CGGCCCCTCA CAGCTGCGGA GCAGGCAGCC 
TTCCGGGATT TCGACTTTGT GGCCGGAGGC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186522.1
CDS	67..2922
Translation	MAADPPLDSELESEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLKREIRKELKLKEGAENLRRATTDLGRSLAPVELLLRGSARRLDLLHQQLQELHAHVVLPDPAAGSDATQSLAEGSPICSSTNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSSKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQALQAGELESQAAPDEAQGDPELGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSGAKAPDRKAVSEAQEKLTESNQKLGLLRESLERRLGELPADHPKGRLLREELTAASSSAFSAILPGPFPATHYSTLSKPAPLTGTLEVRVVGCKNLPETIPWSPPPSVGASGTPESRTPFLSRPARGLYSRSGSLSGRSSLRGEAENATEVSTVLKLDNTVVGQTAWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPIIERIPRLQRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPSAVATGTFSPNASPGAEIRHTGDISMEKLNLGADSDSSSQKSPPGLPSTSCSLSSPTHESTTSPELPSETQETPGPGLCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRSSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTRAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAVFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSTERDAEDVKKQPFFRSLGWDVLLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFRDFDFVAGGY

Target ORF information:

RefSeq Version	NM_001199593.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase N1 (Pkn1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199593.1

ORF Insert Sequence:

ATGGCAGCTG ATCCCCCTTT GGATTCGGAG CTGGAGAGTG AGCCTCGAAG CTGGTCGCTG 
CTGGAACAGC TGGGTCTGGC TGGGGCTGAC CTGGCAGCCC CCGGGGTGCA GCAACAGCTG 
GAGTTAGAGC GAGAGCGACT GAAGCGGGAG ATACGGAAGG AGCTGAAGCT GAAGGAGGGC 
GCTGAGAACC TGAGGCGAGC CACCACTGAC CTGGGCCGTA GCCTGGCCCC TGTGGAGCTG 
CTGCTGCGGG GCTCCGCTCG ACGCCTAGAC TTACTGCACC AGCAGCTGCA GGAGCTGCAT 
GCACATGTGG TGCTGCCTGA CCCTGCAGCT GGAAGCGATG CTACCCAATC CCTTGCAGAG 
GGCAGCCCTA TCTGCTCATC CACCAACCTG AGCAGAGTGG CTGGGCTGGA GAAGCAGCTG 
GCCATTGAGC TCAAGGTCAA ACAGGGGGCA GAGAACATGA TCCAGACCTA TAGCAATGGC 
AGCAGCAAGG ACCGGAAGCT GTTGTTGACA GCCCAGCAGA TGCTGCAGGA CAGTAAGACC 
AAGATTGACA TCATCCGCAT GCAGCTTCGA AGAGCGCTCC AGGCACTACA GGCTGGAGAG 
CTGGAGAGTC AGGCAGCTCC TGATGAAGCC CAAGGAGATC CAGAACTGGG AGCCGTAGAG 
CTACGCATTG AGGAGCTACG ACACCATTTT CGAGTGGAGC ATGCAGTGGC AGAAGGTGCC 
AAGAATGTCC TGCGCCTGCT CAGTGGGGCA AAGGCCCCAG ACCGCAAGGC AGTCAGCGAG 
GCTCAGGAGA AATTGACTGA GTCCAACCAG AAGCTGGGCT TGCTGCGGGA ATCACTGGAG 
AGGCGCCTTG GGGAGTTGCC TGCCGATCAC CCCAAGGGGC GGCTGCTTCG GGAGGAGCTC 
ACTGCAGCCT CGTCCTCAGC CTTCAGCGCC ATACTGCCTG GGCCCTTCCC TGCCACTCAC 
TACAGCACCT TGAGCAAGCC TGCACCGCTC ACAGGGACCC TGGAAGTACG AGTGGTGGGC 
TGCAAAAACC TTCCCGAGAC CATCCCCTGG AGCCCTCCCC CCTCAGTGGG GGCATCTGGG 
ACCCCCGAAA GCCGCACTCC GTTCCTGAGT CGCCCCGCTC GGGGCCTTTA CAGCCGGAGT 
GGAAGCCTTA GTGGACGGAG CAGCCTCAGA GGGGAGGCAG AGAATGCCAC TGAGGTCAGC 
ACCGTGCTCA AGCTGGACAA CACAGTGGTG GGGCAAACAG CCTGGAAGCC ATGCGGCCCC 
AATGCCTGGG ACCAGAGCTT TACCCTGGAG CTGGAGAGGG CTCGGGAGCT GGAGCTGGCT 
GTGTTCTGGC GAGACCAGAG GGGTCTGTGT GCTCTCAAAT TTCTGAAGTT GGAGGACTTC 
TTGGACAACG AGAGGCATGA GGTGCAGCTG GACATGGAAC CCCAGGGCTG CCTGGTGGCT 
GAGGTCACTT TCCGAAACCC CATCATCGAG CGGATCCCTA GGCTCCAAAG GCAGAAAAAA 
ATCTTCTCCA AGCAGCAAGG GAAGGCATTT CAGCGAGCCA GACAGATGAA CATAGATGTG 
GCGACTTGGG TGCGGCTGCT CCGTAGACTC ATCCCTAGTG CTGTGGCCAC TGGCACCTTC 
AGTCCCAATG CATCTCCAGG TGCTGAGATC CGGCACACTG GAGACATATC CATGGAGAAA 
TTGAATCTCG GTGCTGACTC AGACAGCTCG TCCCAAAAGA GCCCACCAGG GCTGCCCTCC 
ACCTCATGCA GCCTGAGTTC TCCAACCCAT GAATCCACCA CATCTCCAGA GCTGCCTTCA 
GAGACCCAGG AGACTCCAGG CCCTGGCCTG TGCAGCCCCT TGAGAAAGTC GCCCCTGACA 
CTTGAGGACT TCAAGTTCCT GGCCGTGCTT GGCCGGGGTC ACTTTGGAAA GGTGCTGCTG 
TCTGAATTCC GCTCCAGTGG GGAGCTCTTT GCCATCAAAG CCTTGAAGAA AGGTGACATT 
GTAGCCCGAG ATGAGGTTGA GAGCCTGATG TGTGAGAAGC GGATTTTGGC GGCCGTGACC 
AGGGCAGGAC ATCCCTTCCT GGTGAACCTT TTCGGCTGTT TCCAGACCCC AGAGCACGTG 
TGCTTTGTGA TGGAGTACTC GGCGGGTGGA GACCTGATGC TGCACATTCA TAGCGACGTG 
TTCTCAGAGC CTCGGGCTGT CTTCTATTCG GCCTGTGTGG TGCTGGGACT GCAGTTCCTC 
CATGAACACA AGATTGTCTA CAGGGACCTG AAGTTGGACA ATTTGCTCCT GGATACTGAG 
GGCTACGTCA AGATCGCAGA CTTTGGCCTC TGCAAGGAGG GGATGGGCTA TGGGGACCGG 
ACCAGCACGT TCTGCGGAAC TCCGGAGTTC CTGGCGCCGG AAGTGCTCAC AGACACATCC 
TACACGCGAG CAGTGGACTG GTGGGGACTG GGCGTGCTGC TCTATGAGAT GTTGGTTGGA 
GAGTCTCCGT TCCCTGGGGA TGATGAGGAG GAGGTATTTG ACAGCATTGT CAACGACGAA 
GTTCGCTATC CCCGCTTCCT GTCTGCAGAG GCCATCGGCA TCATGAGAAG GCTACTGCGG 
AGGAACCCGG AGCGGAGGCT GGGGTCCACT GAGCGCGATG CAGAAGATGT GAAAAAACAG 
CCTTTCTTCC GGTCTCTGGG CTGGGATGTC CTGCTGGCCC GCCGCTTGCC TCCACCCTTC 
GTGCCTACAC TTTCAGGGCG CACAGATGTC AGCAACTTCG ATGAGGAGTT CACTGGGGAG 
GCCCCCACAC TGAGTCCTCC CCGGGATGCA CGGCCCCTCA CAGCTGCGGA GCAGGCAGCC 
TTCCGGGATT TCGACTTTGT GGCCGGAGGC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkn1 ( XM_030243621.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099481.1 Mus musculus protein kinase N1 (Pkn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu02273
Clone ID Related Accession (Same CDS sequence)	XM_030243621.1 , NM_001199593.1
Accession Version	XM_030243621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase N1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTG ATCCCCCTTT GGATTCGGAG CTGGAGAGTG AGCCTCGAAG CTGGTCGCTG 
CTGGAACAGC TGGGTCTGGC TGGGGCTGAC CTGGCAGCCC CCGGGGTGCA GCAACAGCTG 
GAGTTAGAGC GAGAGCGACT GAAGCGGGAG ATACGGAAGG AGCTGAAGCT GAAGGAGGGC 
GCTGAGAACC TGAGGCGAGC CACCACTGAC CTGGGCCGTA GCCTGGCCCC TGTGGAGCTG 
CTGCTGCGGG GCTCCGCTCG ACGCCTAGAC TTACTGCACC AGCAGCTGCA GGAGCTGCAT 
GCACATGTGG TGCTGCCTGA CCCTGCAGCT GGAAGCGATG CTACCCAATC CCTTGCAGAG 
GGCAGCCCTA TCTGCTCATC CACCAACCTG AGCAGAGTGG CTGGGCTGGA GAAGCAGCTG 
GCCATTGAGC TCAAGGTCAA ACAGGGGGCA GAGAACATGA TCCAGACCTA TAGCAATGGC 
AGCAGCAAGG ACCGGAAGCT GTTGTTGACA GCCCAGCAGA TGCTGCAGGA CAGTAAGACC 
AAGATTGACA TCATCCGCAT GCAGCTTCGA AGAGCGCTCC AGGCACTACA GGCTGGAGAG 
CTGGAGAGTC AGGCAGCTCC TGATGAAGCC CAAGGAGATC CAGAACTGGG AGCCGTAGAG 
CTACGCATTG AGGAGCTACG ACACCATTTT CGAGTGGAGC ATGCAGTGGC AGAAGGTGCC 
AAGAATGTCC TGCGCCTGCT CAGTGGGGCA AAGGCCCCAG ACCGCAAGGC AGTCAGCGAG 
GCTCAGGAGA AATTGACTGA GTCCAACCAG AAGCTGGGCT TGCTGCGGGA ATCACTGGAG 
AGGCGCCTTG GGGAGTTGCC TGCCGATCAC CCCAAGGGGC GGCTGCTTCG GGAGGAGCTC 
ACTGCAGCCT CGTCCTCAGC CTTCAGCGCC ATACTGCCTG GGCCCTTCCC TGCCACTCAC 
TACAGCACCT TGAGCAAGCC TGCACCGCTC ACAGGGACCC TGGAAGTACG AGTGGTGGGC 
TGCAAAAACC TTCCCGAGAC CATCCCCTGG AGCCCTCCCC CCTCAGTGGG GGCATCTGGG 
ACCCCCGAAA GCCGCACTCC GTTCCTGAGT CGCCCCGCTC GGGGCCTTTA CAGCCGGAGT 
GGAAGCCTTA GTGGACGGAG CAGCCTCAGA GGGGAGGCAG AGAATGCCAC TGAGGTCAGC 
ACCGTGCTCA AGCTGGACAA CACAGTGGTG GGGCAAACAG CCTGGAAGCC ATGCGGCCCC 
AATGCCTGGG ACCAGAGCTT TACCCTGGAG CTGGAGAGGG CTCGGGAGCT GGAGCTGGCT 
GTGTTCTGGC GAGACCAGAG GGGTCTGTGT GCTCTCAAAT TTCTGAAGTT GGAGGACTTC 
TTGGACAACG AGAGGCATGA GGTGCAGCTG GACATGGAAC CCCAGGGCTG CCTGGTGGCT 
GAGGTCACTT TCCGAAACCC CATCATCGAG CGGATCCCTA GGCTCCAAAG GCAGAAAAAA 
ATCTTCTCCA AGCAGCAAGG GAAGGCATTT CAGCGAGCCA GACAGATGAA CATAGATGTG 
GCGACTTGGG TGCGGCTGCT CCGTAGACTC ATCCCTAGTG CTGTGGCCAC TGGCACCTTC 
AGTCCCAATG CATCTCCAGG TGCTGAGATC CGGCACACTG GAGACATATC CATGGAGAAA 
TTGAATCTCG GTGCTGACTC AGACAGCTCG TCCCAAAAGA GCCCACCAGG GCTGCCCTCC 
ACCTCATGCA GCCTGAGTTC TCCAACCCAT GAATCCACCA CATCTCCAGA GCTGCCTTCA 
GAGACCCAGG AGACTCCAGG CCCTGGCCTG TGCAGCCCCT TGAGAAAGTC GCCCCTGACA 
CTTGAGGACT TCAAGTTCCT GGCCGTGCTT GGCCGGGGTC ACTTTGGAAA GGTGCTGCTG 
TCTGAATTCC GCTCCAGTGG GGAGCTCTTT GCCATCAAAG CCTTGAAGAA AGGTGACATT 
GTAGCCCGAG ATGAGGTTGA GAGCCTGATG TGTGAGAAGC GGATTTTGGC GGCCGTGACC 
AGGGCAGGAC ATCCCTTCCT GGTGAACCTT TTCGGCTGTT TCCAGACCCC AGAGCACGTG 
TGCTTTGTGA TGGAGTACTC GGCGGGTGGA GACCTGATGC TGCACATTCA TAGCGACGTG 
TTCTCAGAGC CTCGGGCTGT CTTCTATTCG GCCTGTGTGG TGCTGGGACT GCAGTTCCTC 
CATGAACACA AGATTGTCTA CAGGGACCTG AAGTTGGACA ATTTGCTCCT GGATACTGAG 
GGCTACGTCA AGATCGCAGA CTTTGGCCTC TGCAAGGAGG GGATGGGCTA TGGGGACCGG 
ACCAGCACGT TCTGCGGAAC TCCGGAGTTC CTGGCGCCGG AAGTGCTCAC AGACACATCC 
TACACGCGAG CAGTGGACTG GTGGGGACTG GGCGTGCTGC TCTATGAGAT GTTGGTTGGA 
GAGTCTCCGT TCCCTGGGGA TGATGAGGAG GAGGTATTTG ACAGCATTGT CAACGACGAA 
GTTCGCTATC CCCGCTTCCT GTCTGCAGAG GCCATCGGCA TCATGAGAAG GCTACTGCGG 
AGGAACCCGG AGCGGAGGCT GGGGTCCACT GAGCGCGATG CAGAAGATGT GAAAAAACAG 
CCTTTCTTCC GGTCTCTGGG CTGGGATGTC CTGCTGGCCC GCCGCTTGCC TCCACCCTTC 
GTGCCTACAC TTTCAGGGCG CACAGATGTC AGCAACTTCG ATGAGGAGTT CACTGGGGAG 
GCCCCCACAC TGAGTCCTCC CCGGGATGCA CGGCCCCTCA CAGCTGCGGA GCAGGCAGCC 
TTCCGGGATT TCGACTTTGT GGCCGGAGGC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099481.1
CDS	337..3192
Translation	MAADPPLDSELESEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLKREIRKELKLKEGAENLRRATTDLGRSLAPVELLLRGSARRLDLLHQQLQELHAHVVLPDPAAGSDATQSLAEGSPICSSTNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSSKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQALQAGELESQAAPDEAQGDPELGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSGAKAPDRKAVSEAQEKLTESNQKLGLLRESLERRLGELPADHPKGRLLREELTAASSSAFSAILPGPFPATHYSTLSKPAPLTGTLEVRVVGCKNLPETIPWSPPPSVGASGTPESRTPFLSRPARGLYSRSGSLSGRSSLRGEAENATEVSTVLKLDNTVVGQTAWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPIIERIPRLQRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPSAVATGTFSPNASPGAEIRHTGDISMEKLNLGADSDSSSQKSPPGLPSTSCSLSSPTHESTTSPELPSETQETPGPGLCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRSSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTRAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAVFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSTERDAEDVKKQPFFRSLGWDVLLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFRDFDFVAGGY

Target ORF information:

RefSeq Version	XM_030243621.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase N1 (Pkn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243621.1

ORF Insert Sequence:

ATGGCAGCTG ATCCCCCTTT GGATTCGGAG CTGGAGAGTG AGCCTCGAAG CTGGTCGCTG 
CTGGAACAGC TGGGTCTGGC TGGGGCTGAC CTGGCAGCCC CCGGGGTGCA GCAACAGCTG 
GAGTTAGAGC GAGAGCGACT GAAGCGGGAG ATACGGAAGG AGCTGAAGCT GAAGGAGGGC 
GCTGAGAACC TGAGGCGAGC CACCACTGAC CTGGGCCGTA GCCTGGCCCC TGTGGAGCTG 
CTGCTGCGGG GCTCCGCTCG ACGCCTAGAC TTACTGCACC AGCAGCTGCA GGAGCTGCAT 
GCACATGTGG TGCTGCCTGA CCCTGCAGCT GGAAGCGATG CTACCCAATC CCTTGCAGAG 
GGCAGCCCTA TCTGCTCATC CACCAACCTG AGCAGAGTGG CTGGGCTGGA GAAGCAGCTG 
GCCATTGAGC TCAAGGTCAA ACAGGGGGCA GAGAACATGA TCCAGACCTA TAGCAATGGC 
AGCAGCAAGG ACCGGAAGCT GTTGTTGACA GCCCAGCAGA TGCTGCAGGA CAGTAAGACC 
AAGATTGACA TCATCCGCAT GCAGCTTCGA AGAGCGCTCC AGGCACTACA GGCTGGAGAG 
CTGGAGAGTC AGGCAGCTCC TGATGAAGCC CAAGGAGATC CAGAACTGGG AGCCGTAGAG 
CTACGCATTG AGGAGCTACG ACACCATTTT CGAGTGGAGC ATGCAGTGGC AGAAGGTGCC 
AAGAATGTCC TGCGCCTGCT CAGTGGGGCA AAGGCCCCAG ACCGCAAGGC AGTCAGCGAG 
GCTCAGGAGA AATTGACTGA GTCCAACCAG AAGCTGGGCT TGCTGCGGGA ATCACTGGAG 
AGGCGCCTTG GGGAGTTGCC TGCCGATCAC CCCAAGGGGC GGCTGCTTCG GGAGGAGCTC 
ACTGCAGCCT CGTCCTCAGC CTTCAGCGCC ATACTGCCTG GGCCCTTCCC TGCCACTCAC 
TACAGCACCT TGAGCAAGCC TGCACCGCTC ACAGGGACCC TGGAAGTACG AGTGGTGGGC 
TGCAAAAACC TTCCCGAGAC CATCCCCTGG AGCCCTCCCC CCTCAGTGGG GGCATCTGGG 
ACCCCCGAAA GCCGCACTCC GTTCCTGAGT CGCCCCGCTC GGGGCCTTTA CAGCCGGAGT 
GGAAGCCTTA GTGGACGGAG CAGCCTCAGA GGGGAGGCAG AGAATGCCAC TGAGGTCAGC 
ACCGTGCTCA AGCTGGACAA CACAGTGGTG GGGCAAACAG CCTGGAAGCC ATGCGGCCCC 
AATGCCTGGG ACCAGAGCTT TACCCTGGAG CTGGAGAGGG CTCGGGAGCT GGAGCTGGCT 
GTGTTCTGGC GAGACCAGAG GGGTCTGTGT GCTCTCAAAT TTCTGAAGTT GGAGGACTTC 
TTGGACAACG AGAGGCATGA GGTGCAGCTG GACATGGAAC CCCAGGGCTG CCTGGTGGCT 
GAGGTCACTT TCCGAAACCC CATCATCGAG CGGATCCCTA GGCTCCAAAG GCAGAAAAAA 
ATCTTCTCCA AGCAGCAAGG GAAGGCATTT CAGCGAGCCA GACAGATGAA CATAGATGTG 
GCGACTTGGG TGCGGCTGCT CCGTAGACTC ATCCCTAGTG CTGTGGCCAC TGGCACCTTC 
AGTCCCAATG CATCTCCAGG TGCTGAGATC CGGCACACTG GAGACATATC CATGGAGAAA 
TTGAATCTCG GTGCTGACTC AGACAGCTCG TCCCAAAAGA GCCCACCAGG GCTGCCCTCC 
ACCTCATGCA GCCTGAGTTC TCCAACCCAT GAATCCACCA CATCTCCAGA GCTGCCTTCA 
GAGACCCAGG AGACTCCAGG CCCTGGCCTG TGCAGCCCCT TGAGAAAGTC GCCCCTGACA 
CTTGAGGACT TCAAGTTCCT GGCCGTGCTT GGCCGGGGTC ACTTTGGAAA GGTGCTGCTG 
TCTGAATTCC GCTCCAGTGG GGAGCTCTTT GCCATCAAAG CCTTGAAGAA AGGTGACATT 
GTAGCCCGAG ATGAGGTTGA GAGCCTGATG TGTGAGAAGC GGATTTTGGC GGCCGTGACC 
AGGGCAGGAC ATCCCTTCCT GGTGAACCTT TTCGGCTGTT TCCAGACCCC AGAGCACGTG 
TGCTTTGTGA TGGAGTACTC GGCGGGTGGA GACCTGATGC TGCACATTCA TAGCGACGTG 
TTCTCAGAGC CTCGGGCTGT CTTCTATTCG GCCTGTGTGG TGCTGGGACT GCAGTTCCTC 
CATGAACACA AGATTGTCTA CAGGGACCTG AAGTTGGACA ATTTGCTCCT GGATACTGAG 
GGCTACGTCA AGATCGCAGA CTTTGGCCTC TGCAAGGAGG GGATGGGCTA TGGGGACCGG 
ACCAGCACGT TCTGCGGAAC TCCGGAGTTC CTGGCGCCGG AAGTGCTCAC AGACACATCC 
TACACGCGAG CAGTGGACTG GTGGGGACTG GGCGTGCTGC TCTATGAGAT GTTGGTTGGA 
GAGTCTCCGT TCCCTGGGGA TGATGAGGAG GAGGTATTTG ACAGCATTGT CAACGACGAA 
GTTCGCTATC CCCGCTTCCT GTCTGCAGAG GCCATCGGCA TCATGAGAAG GCTACTGCGG 
AGGAACCCGG AGCGGAGGCT GGGGTCCACT GAGCGCGATG CAGAAGATGT GAAAAAACAG 
CCTTTCTTCC GGTCTCTGGG CTGGGATGTC CTGCTGGCCC GCCGCTTGCC TCCACCCTTC 
GTGCCTACAC TTTCAGGGCG CACAGATGTC AGCAACTTCG ATGAGGAGTT CACTGGGGAG 
GCCCCCACAC TGAGTCCTCC CCGGGATGCA CGGCCCCTCA CAGCTGCGGA GCAGGCAGCC 
TTCCGGGATT TCGACTTTGT GGCCGGAGGC TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkn1 ( NM_177262.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796236.2 Mus musculus protein kinase N1 (Pkn1), transcript variant 2, mRNA.

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CloneID	OMu19239
Clone ID Related Accession (Same CDS sequence)	NM_177262.4
Accession Version	NM_177262.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase N1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC164432.3, BC052923.1 and CX568360.1. On Dec 9, 2010 this sequence version replaced NM_177262.3. Transcript Variant: This variant (2) contains a different segment for its 5' UTR and 5' coding region, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct N-terminus when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170556.1, BC052923.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGGCG ACGCCGTACA GAGTGAGCCT CGAAGCTGGT CGCTGCTGGA ACAGCTGGGT 
CTGGCTGGGG CTGACCTGGC AGCCCCCGGG GTGCAGCAAC AGCTGGAGTT AGAGCGAGAG 
CGACTGAAGC GGGAGATACG GAAGGAGCTG AAGCTGAAGG AGGGCGCTGA GAACCTGAGG 
CGAGCCACCA CTGACCTGGG CCGTAGCCTG GCCCCTGTGG AGCTGCTGCT GCGGGGCTCC 
GCTCGACGCC TAGACTTACT GCACCAGCAG CTGCAGGAGC TGCATGCACA TGTGGTGCTG 
CCTGACCCTG CAGCTGGAAG CGATGCTACC CAATCCCTTG CAGAGGGCAG CCCTATCTGC 
TCATCCACCA ACCTGAGCAG AGTGGCTGGG CTGGAGAAGC AGCTGGCCAT TGAGCTCAAG 
GTCAAACAGG GGGCAGAGAA CATGATCCAG ACCTATAGCA ATGGCAGCAG CAAGGACCGG 
AAGCTGTTGT TGACAGCCCA GCAGATGCTG CAGGACAGTA AGACCAAGAT TGACATCATC 
CGCATGCAGC TTCGAAGAGC GCTCCAGGCA CTACAGGCTG GAGAGCTGGA GAGTCAGGCA 
GCTCCTGATG AAGCCCAAGG AGATCCAGAA CTGGGAGCCG TAGAGCTACG CATTGAGGAG 
CTACGACACC ATTTTCGAGT GGAGCATGCA GTGGCAGAAG GTGCCAAGAA TGTCCTGCGC 
CTGCTCAGTG GGGCAAAGGC CCCAGACCGC AAGGCAGTCA GCGAGGCTCA GGAGAAATTG 
ACTGAGTCCA ACCAGAAGCT GGGCTTGCTG CGGGAATCAC TGGAGAGGCG CCTTGGGGAG 
TTGCCTGCCG ATCACCCCAA GGGGCGGCTG CTTCGGGAGG AGCTCACTGC AGCCTCGTCC 
TCAGCCTTCA GCGCCATACT GCCTGGGCCC TTCCCTGCCA CTCACTACAG CACCTTGAGC 
AAGCCTGCAC CGCTCACAGG GACCCTGGAA GTACGAGTGG TGGGCTGCAA AAACCTTCCC 
GAGACCATCC CCTGGAGCCC TCCCCCCTCA GTGGGGGCAT CTGGGACCCC CGAAAGCCGC 
ACTCCGTTCC TGAGTCGCCC CGCTCGGGGC CTTTACAGCC GGAGTGGAAG CCTTAGTGGA 
CGGAGCAGCC TCAGAGGGGA GGCAGAGAAT GCCACTGAGG TCAGCACCGT GCTCAAGCTG 
GACAACACAG TGGTGGGGCA AACAGCCTGG AAGCCATGCG GCCCCAATGC CTGGGACCAG 
AGCTTTACCC TGGAGCTGGA GAGGGCTCGG GAGCTGGAGC TGGCTGTGTT CTGGCGAGAC 
CAGAGGGGTC TGTGTGCTCT CAAATTTCTG AAGTTGGAGG ACTTCTTGGA CAACGAGAGG 
CATGAGGTGC AGCTGGACAT GGAACCCCAG GGCTGCCTGG TGGCTGAGGT CACTTTCCGA 
AACCCCATCA TCGAGCGGAT CCCTAGGCTC CAAAGGCAGA AAAAAATCTT CTCCAAGCAG 
CAAGGGAAGG CATTTCAGCG AGCCAGACAG ATGAACATAG ATGTGGCGAC TTGGGTGCGG 
CTGCTCCGTA GACTCATCCC TAGTGCTGTG GCCACTGGCA CCTTCAGTCC CAATGCATCT 
CCAGGTGCTG AGATCCGGCA CACTGGAGAC ATATCCATGG AGAAATTGAA TCTCGGTGCT 
GACTCAGACA GCTCGTCCCA AAAGAGCCCA CCAGGGCTGC CCTCCACCTC ATGCAGCCTG 
AGTTCTCCAA CCCATGAATC CACCACATCT CCAGAGCTGC CTTCAGAGAC CCAGGAGACT 
CCAGGCCCTG GCCTGTGCAG CCCCTTGAGA AAGTCGCCCC TGACACTTGA GGACTTCAAG 
TTCCTGGCCG TGCTTGGCCG GGGTCACTTT GGAAAGGTGC TGCTGTCTGA ATTCCGCTCC 
AGTGGGGAGC TCTTTGCCAT CAAAGCCTTG AAGAAAGGTG ACATTGTAGC CCGAGATGAG 
GTTGAGAGCC TGATGTGTGA GAAGCGGATT TTGGCGGCCG TGACCAGGGC AGGACATCCC 
TTCCTGGTGA ACCTTTTCGG CTGTTTCCAG ACCCCAGAGC ACGTGTGCTT TGTGATGGAG 
TACTCGGCGG GTGGAGACCT GATGCTGCAC ATTCATAGCG ACGTGTTCTC AGAGCCTCGG 
GCTGTCTTCT ATTCGGCCTG TGTGGTGCTG GGACTGCAGT TCCTCCATGA ACACAAGATT 
GTCTACAGGG ACCTGAAGTT GGACAATTTG CTCCTGGATA CTGAGGGCTA CGTCAAGATC 
GCAGACTTTG GCCTCTGCAA GGAGGGGATG GGCTATGGGG ACCGGACCAG CACGTTCTGC 
GGAACTCCGG AGTTCCTGGC GCCGGAAGTG CTCACAGACA CATCCTACAC GCGAGCAGTG 
GACTGGTGGG GACTGGGCGT GCTGCTCTAT GAGATGTTGG TTGGAGAGTC TCCGTTCCCT 
GGGGATGATG AGGAGGAGGT ATTTGACAGC ATTGTCAACG ACGAAGTTCG CTATCCCCGC 
TTCCTGTCTG CAGAGGCCAT CGGCATCATG AGAAGGCTAC TGCGGAGGAA CCCGGAGCGG 
AGGCTGGGGT CCACTGAGCG CGATGCAGAA GATGTGAAAA AACAGCCTTT CTTCCGGTCT 
CTGGGCTGGG ATGTCCTGCT GGCCCGCCGC TTGCCTCCAC CCTTCGTGCC TACACTTTCA 
GGGCGCACAG ATGTCAGCAA CTTCGATGAG GAGTTCACTG GGGAGGCCCC CACACTGAGT 
CCTCCCCGGG ATGCACGGCC CCTCACAGCT GCGGAGCAGG CAGCCTTCCG GGATTTCGAC 
TTTGTGGCCG GAGGCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796236.2
CDS	193..3033
Translation	MAGDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLKREIRKELKLKEGAENLRRATTDLGRSLAPVELLLRGSARRLDLLHQQLQELHAHVVLPDPAAGSDATQSLAEGSPICSSTNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSSKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQALQAGELESQAAPDEAQGDPELGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSGAKAPDRKAVSEAQEKLTESNQKLGLLRESLERRLGELPADHPKGRLLREELTAASSSAFSAILPGPFPATHYSTLSKPAPLTGTLEVRVVGCKNLPETIPWSPPPSVGASGTPESRTPFLSRPARGLYSRSGSLSGRSSLRGEAENATEVSTVLKLDNTVVGQTAWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPIIERIPRLQRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPSAVATGTFSPNASPGAEIRHTGDISMEKLNLGADSDSSSQKSPPGLPSTSCSLSSPTHESTTSPELPSETQETPGPGLCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRSSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTRAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAVFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSTERDAEDVKKQPFFRSLGWDVLLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFRDFDFVAGGY

Target ORF information:

RefSeq Version	NM_177262.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase N1 (Pkn1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177262.4

ORF Insert Sequence:

ATGGCCGGCG ACGCCGTACA GAGTGAGCCT CGAAGCTGGT CGCTGCTGGA ACAGCTGGGT 
CTGGCTGGGG CTGACCTGGC AGCCCCCGGG GTGCAGCAAC AGCTGGAGTT AGAGCGAGAG 
CGACTGAAGC GGGAGATACG GAAGGAGCTG AAGCTGAAGG AGGGCGCTGA GAACCTGAGG 
CGAGCCACCA CTGACCTGGG CCGTAGCCTG GCCCCTGTGG AGCTGCTGCT GCGGGGCTCC 
GCTCGACGCC TAGACTTACT GCACCAGCAG CTGCAGGAGC TGCATGCACA TGTGGTGCTG 
CCTGACCCTG CAGCTGGAAG CGATGCTACC CAATCCCTTG CAGAGGGCAG CCCTATCTGC 
TCATCCACCA ACCTGAGCAG AGTGGCTGGG CTGGAGAAGC AGCTGGCCAT TGAGCTCAAG 
GTCAAACAGG GGGCAGAGAA CATGATCCAG ACCTATAGCA ATGGCAGCAG CAAGGACCGG 
AAGCTGTTGT TGACAGCCCA GCAGATGCTG CAGGACAGTA AGACCAAGAT TGACATCATC 
CGCATGCAGC TTCGAAGAGC GCTCCAGGCA CTACAGGCTG GAGAGCTGGA GAGTCAGGCA 
GCTCCTGATG AAGCCCAAGG AGATCCAGAA CTGGGAGCCG TAGAGCTACG CATTGAGGAG 
CTACGACACC ATTTTCGAGT GGAGCATGCA GTGGCAGAAG GTGCCAAGAA TGTCCTGCGC 
CTGCTCAGTG GGGCAAAGGC CCCAGACCGC AAGGCAGTCA GCGAGGCTCA GGAGAAATTG 
ACTGAGTCCA ACCAGAAGCT GGGCTTGCTG CGGGAATCAC TGGAGAGGCG CCTTGGGGAG 
TTGCCTGCCG ATCACCCCAA GGGGCGGCTG CTTCGGGAGG AGCTCACTGC AGCCTCGTCC 
TCAGCCTTCA GCGCCATACT GCCTGGGCCC TTCCCTGCCA CTCACTACAG CACCTTGAGC 
AAGCCTGCAC CGCTCACAGG GACCCTGGAA GTACGAGTGG TGGGCTGCAA AAACCTTCCC 
GAGACCATCC CCTGGAGCCC TCCCCCCTCA GTGGGGGCAT CTGGGACCCC CGAAAGCCGC 
ACTCCGTTCC TGAGTCGCCC CGCTCGGGGC CTTTACAGCC GGAGTGGAAG CCTTAGTGGA 
CGGAGCAGCC TCAGAGGGGA GGCAGAGAAT GCCACTGAGG TCAGCACCGT GCTCAAGCTG 
GACAACACAG TGGTGGGGCA AACAGCCTGG AAGCCATGCG GCCCCAATGC CTGGGACCAG 
AGCTTTACCC TGGAGCTGGA GAGGGCTCGG GAGCTGGAGC TGGCTGTGTT CTGGCGAGAC 
CAGAGGGGTC TGTGTGCTCT CAAATTTCTG AAGTTGGAGG ACTTCTTGGA CAACGAGAGG 
CATGAGGTGC AGCTGGACAT GGAACCCCAG GGCTGCCTGG TGGCTGAGGT CACTTTCCGA 
AACCCCATCA TCGAGCGGAT CCCTAGGCTC CAAAGGCAGA AAAAAATCTT CTCCAAGCAG 
CAAGGGAAGG CATTTCAGCG AGCCAGACAG ATGAACATAG ATGTGGCGAC TTGGGTGCGG 
CTGCTCCGTA GACTCATCCC TAGTGCTGTG GCCACTGGCA CCTTCAGTCC CAATGCATCT 
CCAGGTGCTG AGATCCGGCA CACTGGAGAC ATATCCATGG AGAAATTGAA TCTCGGTGCT 
GACTCAGACA GCTCGTCCCA AAAGAGCCCA CCAGGGCTGC CCTCCACCTC ATGCAGCCTG 
AGTTCTCCAA CCCATGAATC CACCACATCT CCAGAGCTGC CTTCAGAGAC CCAGGAGACT 
CCAGGCCCTG GCCTGTGCAG CCCCTTGAGA AAGTCGCCCC TGACACTTGA GGACTTCAAG 
TTCCTGGCCG TGCTTGGCCG GGGTCACTTT GGAAAGGTGC TGCTGTCTGA ATTCCGCTCC 
AGTGGGGAGC TCTTTGCCAT CAAAGCCTTG AAGAAAGGTG ACATTGTAGC CCGAGATGAG 
GTTGAGAGCC TGATGTGTGA GAAGCGGATT TTGGCGGCCG TGACCAGGGC AGGACATCCC 
TTCCTGGTGA ACCTTTTCGG CTGTTTCCAG ACCCCAGAGC ACGTGTGCTT TGTGATGGAG 
TACTCGGCGG GTGGAGACCT GATGCTGCAC ATTCATAGCG ACGTGTTCTC AGAGCCTCGG 
GCTGTCTTCT ATTCGGCCTG TGTGGTGCTG GGACTGCAGT TCCTCCATGA ACACAAGATT 
GTCTACAGGG ACCTGAAGTT GGACAATTTG CTCCTGGATA CTGAGGGCTA CGTCAAGATC 
GCAGACTTTG GCCTCTGCAA GGAGGGGATG GGCTATGGGG ACCGGACCAG CACGTTCTGC 
GGAACTCCGG AGTTCCTGGC GCCGGAAGTG CTCACAGACA CATCCTACAC GCGAGCAGTG 
GACTGGTGGG GACTGGGCGT GCTGCTCTAT GAGATGTTGG TTGGAGAGTC TCCGTTCCCT 
GGGGATGATG AGGAGGAGGT ATTTGACAGC ATTGTCAACG ACGAAGTTCG CTATCCCCGC 
TTCCTGTCTG CAGAGGCCAT CGGCATCATG AGAAGGCTAC TGCGGAGGAA CCCGGAGCGG 
AGGCTGGGGT CCACTGAGCG CGATGCAGAA GATGTGAAAA AACAGCCTTT CTTCCGGTCT 
CTGGGCTGGG ATGTCCTGCT GGCCCGCCGC TTGCCTCCAC CCTTCGTGCC TACACTTTCA 
GGGCGCACAG ATGTCAGCAA CTTCGATGAG GAGTTCACTG GGGAGGCCCC CACACTGAGT 
CCTCCCCGGG ATGCACGGCC CCTCACAGCT GCGGAGCAGG CAGCCTTCCG GGATTTCGAC 
TTTGTGGCCG GAGGCTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The homeoprotein Msx1 cooperates with Pkn1 to prevent terminal differentiation in myogenic precursor cells.
Biochimie16255-65(2019 Jul)
Zhu X,Li M,Jia X,Hou W,Yang J,Zhao H,Wang G,Wang J

Protein kinase N1 critically regulates cerebellar development and long-term function.
The Journal of clinical investigation128(5)2076-2088(2018 05)
zur Nedden S,Eith R,Schwarzer C,Zanetti L,Seitter H,Fresser F,Koschak A,Cameron AJ,Parker PJ,Baier G,Baier-Bitterlich G

Loss of Protein Kinase Novel 1 (PKN1) is associated with mild systolic and diastolic contractile dysfunction, increased phospholamban Thr17 phosphorylation, and exacerbated ischaemia-reperfusion injury.
Cardiovascular research114(1)138-157(2018 01)
Francois AA,Obasanjo-Blackshire K,Clark JE,Boguslavskyi A,Holt MR,Parker PJ,Marber MS,Heads RJ

The protein kinase C super-family member PKN is regulated by mTOR and influences differentiation during prostate cancer progression.
The Prostate77(15)1452-1467(2017 Nov)
Yang CS,Melhuish TA,Spencer A,Ni L,Hao Y,Jividen K,Harris TE,Snow C,Frierson HF Jr,Wotton D,Paschal BM

Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1.
Scientific reports7(1)7663(2017 08)
Mashud R,Nomachi A,Hayakawa A,Kubouchi K,Danno S,Hirata T,Matsuo K,Nakayama T,Satoh R,Sugiura R,Abe M,Sakimura K,Wakana S,Ohsaki H,Kamoshida S,Mukai H

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Pkn1 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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