pkn1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pkn1a
Entrez Gene ID	560840
Full Name	protein kinase N1a
Synonyms	pkn1,si:ch211-214c7.6,wu:fb69d01
General protein information	Preferred Names protein kinase N1a Names serine/threonine-protein kinase N1 protein kinase N1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160050.3	XP_005160107.2	serine/threonine-protein kinase N1 isoform X1
XM_005160051.4	XP_005160108.1	serine/threonine-protein kinase N1 isoform X3
XM_005160050.4	XP_005160107.3	serine/threonine-protein kinase N1 isoform X1
XM_005160051.3	XP_005160108.1	serine/threonine-protein kinase N1 isoform X3
XM_684239.6	XP_689331.3	serine/threonine-protein kinase N1 isoform X2
XM_684239.7	XP_689331.3	serine/threonine-protein kinase N1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PKN1	XP_512443.3
Danio rerio (zebrafish)	LOC560840	XP_005160108.1
Homo sapiens (human)	PKN1	NP_002732.3
Bos taurus (cattle)	PKN1	NP_001073715.1
Rattus norvegicus (Norway rat)	Pkn1	NP_058871.2
Canis lupus familiaris (dog)	PKN1	XP_003639827.1
Danio rerio (zebrafish)	zgc:162290	NP_001082929.1
Macaca mulatta (Rhesus monkey)	LOC718834	XP_002808221.1
Mus musculus (house mouse)	Pkn1	NP_796236.2

Gene Ontology

The following pkn1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pkn1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa06096	XM_684239.7 Latest version!	Danio rerio protein kinase N1a (pkn1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa06097	XM_005160051.4 Latest version!	Danio rerio protein kinase N1a (pkn1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa147438	XM_005160050.4 Latest version!	Danio rerio protein kinase N1a (pkn1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa43355	XM_005160050.3 Latest version!	Danio rerio protein kinase N1a (pkn1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa06097	XM_005160051.3	Danio rerio protein kinase N1a (pkn1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa06096	XM_684239.6	Danio rerio protein kinase N1a (pkn1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pkn1a ( XM_684239.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_689331.3 Danio rerio protein kinase N1a (pkn1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa06096
Clone ID Related Accession (Same CDS sequence)	XM_684239.6 , XM_684239.7
Accession Version	XM_684239.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase N1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_684239.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCG AGGAGGGAAG AAATACATCC CTCACAGAGC AGCACGACGA CGACACCATC 
ACACAGACTG AGACTCCGAG TGATCCCGGT GGGTTGTCTG GATTAGAGCG GCTTGGCTTG 
GACCACAGCT CTGATTACTC TGACTCGAGC GTACAACAGC GACTGGATAA AGAGCGCGAG 
CGAATCCGTC GGGAAATCCG CAAGGAGCTG AAGATTAAAG AGGGGGCAGA GAATCTCCGC 
AGAGCCACTA CAGATAAACG AAATGCTCAG CAGGTGGACA GCCAGTTGCG CTGCTCAAAT 
CGGCGTCTGG ACTCACTCCA TGCTGAATTA CAAGAACTGG ACGCTCATAT TGTGGTCAAA 
GGGACCGATG ATACACACGA TGACGCTCCT CAGTCTCCGG GAGCGGAGAG CAGGTCATCG 
GCACACAAGG AGAGAATTGC TGCTTTGGAA AGGCAGTTAA ACATCGAGCT CAAAGTCAAA 
CAGGGGGCAG AAAACATGAT CCCCATTTAT ACCAACGGTT CCACCAAGGA CAAGAAGATG 
TTGCAAACAG CTCAGCAGAT GCTACAGGAC AGCAAGACAA AGATTGACAT CATCCGCATG 
CAGATCCGGA AAGCAGTGCA GGCCTACGAA CACAACGATG ATACACAGGG AAAGCCTGAT 
CCATGTGGTA TGGAGCTACG CATTGAAGAG CTCAGACACC ATTACCGAGT AGAGCACGCC 
GTCGCTGAGG GAGCCAAAAA CGTCCTTAGA CTTCTTGGAG CCAGTAAAGT ACAAGACAAG 
AAGGCCATGT CAGAGGCTCA GTGTCGTCTG AGTGAATCGT CGCAAAGGTT GGACCTGCTG 
CGAGATTCTT TAGATCGACG GTTAGCAGAG CTTCCTGAGG ATCATCCCAA AGCTTCTGTT 
ATCAAAGAGG AGCTGGTTTT AGCATCCTCA CCAGCCTTCA GCTCTCAACA CAGCGCCCCC 
TACCTGCACA ACCAGTACAG CACTCTCTCC AAACCCTCAC CCCTCACTGG TCGGCTCCAG 
GTGACATTAT TGGGCTGTAT GGGTCTTCTG GAGGTGGTTC CCGGACGCTC TCGTGGTAGC 
CAGGTGGTTT TACCGTGTTA CAGTCCAGGA GAGGGACGAT CATTCATGCG CAGTGGAAAA 
GGCCTGTATA GTCGCAGTGG CAGCGTTAGT GGAAAAACAC CCCTCAAAAC CGACGAACTC 
TCCTCGGATG TGAGCGCAGT ATTGAAGCTG GACAACACTG TGGTGTGTCA GACGGCATGG 
AAGATGGTTG GAGAACAGGG CTGGGATCAG ACCTTCATCA TCGAGCTGGA GAGATCCAGA 
GAGATGGAGA TTGCCGTGTA CTGGAGAGAC TACCGCTCTC TGTGTGCGCT GAAATTTCTC 
AAGCTGGAGG ATTTCTTGGA CAACCAGAAA CACAAAGTAC AACTAGAGCT AGAACCACAG 
GGACTGCTGC TGGCAGAGGT TACTTTCTTT AACCCTGTGA TTGAGAGGGT TCCACGGCTA 
AAACGGCAGA AGAATGTTTT CTCCAAGCAG CAGGGTAAAG CATTTTTGCG AGCTCGACAG 
ATGAACGTTG ATATTGGCAC ATTCGTGAGG TTACTGAGGA ACGCCATACC AACCGTCAAC 
AACACAGGAA CCTACAGCCC TAACGCGCAC ACTCTACAGA CTGGGGAGAT ATCAGTGGAG 
AAGTTGAGTT TAGATTTTGA TTCACCAATG AAGGTGGACA TCCGGCGGGA GGTGACAGAT 
ATAAGCACTC CGGAGAGGCA GCCGTCCACC GAGCCTCTCT CTGCTCCAGA CGTCACCACG 
CCTATCAGAA CACACTCATT GAGCAGTAAA CAGAAGAAGA CTTCTCTGAG CCTGCAGGAT 
TTCCGACTAA TTGCAGTTCT TGGCAGAGGC CATTTTGGAA AGGTGCTGCT GGCGGAATAT 
AAGAAGTCAG GGAGTATGTA TGCCATTAAA GCTCTAAAGA AAGGAGACAT TGTAGCCAGA 
GATGAAGTAG AAAGTTTGAT GTGTGAGAAG CGTATTTTCG AGGCAGTGAA CGGTGCACAA 
CACCCCTTCC TTGTCAACCT GTTTGCGTGT TTCCAGACTC CAGAACATGT GTGTTTTGTG 
ATGGAGTACA CAGCAGGCGG TGACCTCATG ATGCACATAC ATGCAGACGT CTTCACCGAG 
CCACGGGCTG TGTTTTATTC TGCTTGTGTA GTTTTAGGGC TTCAGTTTTT ACATGACAAC 
AAGATTGTGT ACCGAGATCT TAAATTAGAC AACTTGCTGC TTGACACAGA GGGTTATGTG 
AAGATTGCCG ACTTTGGATT GTGTAAAGAA GGTATGGGTT TTGGGGACCG GACCAGTACG 
TTCTGCGGGA CTCCAGAGTT TCTGGCTCCA GAGGTTTTGA CAGACACATC GTACACCCGT 
GCTGTGGACT GGTGGGGTCT CGGAGTGCTT ATATATGAAA TGTTGGTGGG AGAGTCCCCA 
TTCCCAGGTG ATGACGAAGA GGAAGTTTTT GACAGCATAG TGAATGATGA AGTTCGATAC 
CCCAGGTTCC TGTCCGCAGA GGCCATTGGC ATCATGAGGA GGTTACTAAG GAGAAATCCA 
GAAAGACGAC TGGGCTCCAG TGAGAAAGAT GCAGAAGATG TAAAGAAACA GCCTTTCTTC 
AGGAATATGG ACTGGGAGGC TCTTCTCCTC CGCAAACTCC CTCCTCCTTT TATTCCATCC 
ATCGGAGGAA AAGAAGACGT CAGCAACTTT GATGAGGAGT TCACGACAGA AGCGCCGACT 
CTCACTCCTC CCCGCGAGCC TCGAGTGCTT TCGCGCAAAG ATCAGGACAG TTTCCGGGAC 
TTCGACTATG TGTCTGACCT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_689331.3
CDS	232..3078
Translation	MASEEGRNTSLTEQHDDDTITQTETPSDPGGLSGLERLGLDHSSDYSDSSVQQRLDKERERIRREIRKELKIKEGAENLRRATTDKRNAQQVDSQLRCSNRRLDSLHAELQELDAHIVVKGTDDTHDDAPQSPGAESRSSAHKERIAALERQLNIELKVKQGAENMIPIYTNGSTKDKKMLQTAQQMLQDSKTKIDIIRMQIRKAVQAYEHNDDTQGKPDPCGMELRIEELRHHYRVEHAVAEGAKNVLRLLGASKVQDKKAMSEAQCRLSESSQRLDLLRDSLDRRLAELPEDHPKASVIKEELVLASSPAFSSQHSAPYLHNQYSTLSKPSPLTGRLQVTLLGCMGLLEVVPGRSRGSQVVLPCYSPGEGRSFMRSGKGLYSRSGSVSGKTPLKTDELSSDVSAVLKLDNTVVCQTAWKMVGEQGWDQTFIIELERSREMEIAVYWRDYRSLCALKFLKLEDFLDNQKHKVQLELEPQGLLLAEVTFFNPVIERVPRLKRQKNVFSKQQGKAFLRARQMNVDIGTFVRLLRNAIPTVNNTGTYSPNAHTLQTGEISVEKLSLDFDSPMKVDIRREVTDISTPERQPSTEPLSAPDVTTPIRTHSLSSKQKKTSLSLQDFRLIAVLGRGHFGKVLLAEYKKSGSMYAIKALKKGDIVARDEVESLMCEKRIFEAVNGAQHPFLVNLFACFQTPEHVCFVMEYTAGGDLMMHIHADVFTEPRAVFYSACVVLGLQFLHDNKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGFGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSEKDAEDVKKQPFFRNMDWEALLLRKLPPPFIPSIGGKEDVSNFDEEFTTEAPTLTPPREPRVLSRKDQDSFRDFDYVSDLC

Target ORF information:

RefSeq Version	XM_684239.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein kinase N1a (pkn1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_684239.6

ORF Insert Sequence:

ATGGCCAGCG AGGAGGGAAG AAATACATCC CTCACAGAGC AGCACGACGA CGACACCATC 
ACACAGACTG AGACTCCGAG TGATCCCGGT GGGTTGTCTG GATTAGAGCG GCTTGGCTTG 
GACCACAGCT CTGATTACTC TGACTCGAGC GTACAACAGC GACTGGATAA AGAGCGCGAG 
CGAATCCGTC GGGAAATCCG CAAGGAGCTG AAGATTAAAG AGGGGGCAGA GAATCTCCGC 
AGAGCCACTA CAGATAAACG AAATGCTCAG CAGGTGGACA GCCAGTTGCG CTGCTCAAAT 
CGGCGTCTGG ACTCACTCCA TGCTGAATTA CAAGAACTGG ACGCTCATAT TGTGGTCAAA 
GGGACCGATG ATACACACGA TGACGCTCCT CAGTCTCCGG GAGCGGAGAG CAGGTCATCG 
GCACACAAGG AGAGAATTGC TGCTTTGGAA AGGCAGTTAA ACATCGAGCT CAAAGTCAAA 
CAGGGGGCAG AAAACATGAT CCCCATTTAT ACCAACGGTT CCACCAAGGA CAAGAAGATG 
TTGCAAACAG CTCAGCAGAT GCTACAGGAC AGCAAGACAA AGATTGACAT CATCCGCATG 
CAGATCCGGA AAGCAGTGCA GGCCTACGAA CACAACGATG ATACACAGGG AAAGCCTGAT 
CCATGTGGTA TGGAGCTACG CATTGAAGAG CTCAGACACC ATTACCGAGT AGAGCACGCC 
GTCGCTGAGG GAGCCAAAAA CGTCCTTAGA CTTCTTGGAG CCAGTAAAGT ACAAGACAAG 
AAGGCCATGT CAGAGGCTCA GTGTCGTCTG AGTGAATCGT CGCAAAGGTT GGACCTGCTG 
CGAGATTCTT TAGATCGACG GTTAGCAGAG CTTCCTGAGG ATCATCCCAA AGCTTCTGTT 
ATCAAAGAGG AGCTGGTTTT AGCATCCTCA CCAGCCTTCA GCTCTCAACA CAGCGCCCCC 
TACCTGCACA ACCAGTACAG CACTCTCTCC AAACCCTCAC CCCTCACTGG TCGGCTCCAG 
GTGACATTAT TGGGCTGTAT GGGTCTTCTG GAGGTGGTTC CCGGACGCTC TCGTGGTAGC 
CAGGTGGTTT TACCGTGTTA CAGTCCAGGA GAGGGACGAT CATTCATGCG CAGTGGAAAA 
GGCCTGTATA GTCGCAGTGG CAGCGTTAGT GGAAAAACAC CCCTCAAAAC CGACGAACTC 
TCCTCGGATG TGAGCGCAGT ATTGAAGCTG GACAACACTG TGGTGTGTCA GACGGCATGG 
AAGATGGTTG GAGAACAGGG CTGGGATCAG ACCTTCATCA TCGAGCTGGA GAGATCCAGA 
GAGATGGAGA TTGCCGTGTA CTGGAGAGAC TACCGCTCTC TGTGTGCGCT GAAATTTCTC 
AAGCTGGAGG ATTTCTTGGA CAACCAGAAA CACAAAGTAC AACTAGAGCT AGAACCACAG 
GGACTGCTGC TGGCAGAGGT TACTTTCTTT AACCCTGTGA TTGAGAGGGT TCCACGGCTA 
AAACGGCAGA AGAATGTTTT CTCCAAGCAG CAGGGTAAAG CATTTTTGCG AGCTCGACAG 
ATGAACGTTG ATATTGGCAC ATTCGTGAGG TTACTGAGGA ACGCCATACC AACCGTCAAC 
AACACAGGAA CCTACAGCCC TAACGCGCAC ACTCTACAGA CTGGGGAGAT ATCAGTGGAG 
AAGTTGAGTT TAGATTTTGA TTCACCAATG AAGGTGGACA TCCGGCGGGA GGTGACAGAT 
ATAAGCACTC CGGAGAGGCA GCCGTCCACC GAGCCTCTCT CTGCTCCAGA CGTCACCACG 
CCTATCAGAA CACACTCATT GAGCAGTAAA CAGAAGAAGA CTTCTCTGAG CCTGCAGGAT 
TTCCGACTAA TTGCAGTTCT TGGCAGAGGC CATTTTGGAA AGGTGCTGCT GGCGGAATAT 
AAGAAGTCAG GGAGTATGTA TGCCATTAAA GCTCTAAAGA AAGGAGACAT TGTAGCCAGA 
GATGAAGTAG AAAGTTTGAT GTGTGAGAAG CGTATTTTCG AGGCAGTGAA CGGTGCACAA 
CACCCCTTCC TTGTCAACCT GTTTGCGTGT TTCCAGACTC CAGAACATGT GTGTTTTGTG 
ATGGAGTACA CAGCAGGCGG TGACCTCATG ATGCACATAC ATGCAGACGT CTTCACCGAG 
CCACGGGCTG TGTTTTATTC TGCTTGTGTA GTTTTAGGGC TTCAGTTTTT ACATGACAAC 
AAGATTGTGT ACCGAGATCT TAAATTAGAC AACTTGCTGC TTGACACAGA GGGTTATGTG 
AAGATTGCCG ACTTTGGATT GTGTAAAGAA GGTATGGGTT TTGGGGACCG GACCAGTACG 
TTCTGCGGGA CTCCAGAGTT TCTGGCTCCA GAGGTTTTGA CAGACACATC GTACACCCGT 
GCTGTGGACT GGTGGGGTCT CGGAGTGCTT ATATATGAAA TGTTGGTGGG AGAGTCCCCA 
TTCCCAGGTG ATGACGAAGA GGAAGTTTTT GACAGCATAG TGAATGATGA AGTTCGATAC 
CCCAGGTTCC TGTCCGCAGA GGCCATTGGC ATCATGAGGA GGTTACTAAG GAGAAATCCA 
GAAAGACGAC TGGGCTCCAG TGAGAAAGAT GCAGAAGATG TAAAGAAACA GCCTTTCTTC 
AGGAATATGG ACTGGGAGGC TCTTCTCCTC CGCAAACTCC CTCCTCCTTT TATTCCATCC 
ATCGGAGGAA AAGAAGACGT CAGCAACTTT GATGAGGAGT TCACGACAGA AGCGCCGACT 
CTCACTCCTC CCCGCGAGCC TCGAGTGCTT TCGCGCAAAG ATCAGGACAG TTTCCGGGAC 
TTCGACTATG TGTCTGACCT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkn1a ( XM_684239.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_689331.3 Danio rerio protein kinase N1a (pkn1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa06096
Clone ID Related Accession (Same CDS sequence)	XM_684239.6 , XM_684239.7
Accession Version	XM_684239.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase N1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_684239.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCG AGGAGGGAAG AAATACATCC CTCACAGAGC AGCACGACGA CGACACCATC 
ACACAGACTG AGACTCCGAG TGATCCCGGT GGGTTGTCTG GATTAGAGCG GCTTGGCTTG 
GACCACAGCT CTGATTACTC TGACTCGAGC GTACAACAGC GACTGGATAA AGAGCGCGAG 
CGAATCCGTC GGGAAATCCG CAAGGAGCTG AAGATTAAAG AGGGGGCAGA GAATCTCCGC 
AGAGCCACTA CAGATAAACG AAATGCTCAG CAGGTGGACA GCCAGTTGCG CTGCTCAAAT 
CGGCGTCTGG ACTCACTCCA TGCTGAATTA CAAGAACTGG ACGCTCATAT TGTGGTCAAA 
GGGACCGATG ATACACACGA TGACGCTCCT CAGTCTCCGG GAGCGGAGAG CAGGTCATCG 
GCACACAAGG AGAGAATTGC TGCTTTGGAA AGGCAGTTAA ACATCGAGCT CAAAGTCAAA 
CAGGGGGCAG AAAACATGAT CCCCATTTAT ACCAACGGTT CCACCAAGGA CAAGAAGATG 
TTGCAAACAG CTCAGCAGAT GCTACAGGAC AGCAAGACAA AGATTGACAT CATCCGCATG 
CAGATCCGGA AAGCAGTGCA GGCCTACGAA CACAACGATG ATACACAGGG AAAGCCTGAT 
CCATGTGGTA TGGAGCTACG CATTGAAGAG CTCAGACACC ATTACCGAGT AGAGCACGCC 
GTCGCTGAGG GAGCCAAAAA CGTCCTTAGA CTTCTTGGAG CCAGTAAAGT ACAAGACAAG 
AAGGCCATGT CAGAGGCTCA GTGTCGTCTG AGTGAATCGT CGCAAAGGTT GGACCTGCTG 
CGAGATTCTT TAGATCGACG GTTAGCAGAG CTTCCTGAGG ATCATCCCAA AGCTTCTGTT 
ATCAAAGAGG AGCTGGTTTT AGCATCCTCA CCAGCCTTCA GCTCTCAACA CAGCGCCCCC 
TACCTGCACA ACCAGTACAG CACTCTCTCC AAACCCTCAC CCCTCACTGG TCGGCTCCAG 
GTGACATTAT TGGGCTGTAT GGGTCTTCTG GAGGTGGTTC CCGGACGCTC TCGTGGTAGC 
CAGGTGGTTT TACCGTGTTA CAGTCCAGGA GAGGGACGAT CATTCATGCG CAGTGGAAAA 
GGCCTGTATA GTCGCAGTGG CAGCGTTAGT GGAAAAACAC CCCTCAAAAC CGACGAACTC 
TCCTCGGATG TGAGCGCAGT ATTGAAGCTG GACAACACTG TGGTGTGTCA GACGGCATGG 
AAGATGGTTG GAGAACAGGG CTGGGATCAG ACCTTCATCA TCGAGCTGGA GAGATCCAGA 
GAGATGGAGA TTGCCGTGTA CTGGAGAGAC TACCGCTCTC TGTGTGCGCT GAAATTTCTC 
AAGCTGGAGG ATTTCTTGGA CAACCAGAAA CACAAAGTAC AACTAGAGCT AGAACCACAG 
GGACTGCTGC TGGCAGAGGT TACTTTCTTT AACCCTGTGA TTGAGAGGGT TCCACGGCTA 
AAACGGCAGA AGAATGTTTT CTCCAAGCAG CAGGGTAAAG CATTTTTGCG AGCTCGACAG 
ATGAACGTTG ATATTGGCAC ATTCGTGAGG TTACTGAGGA ACGCCATACC AACCGTCAAC 
AACACAGGAA CCTACAGCCC TAACGCGCAC ACTCTACAGA CTGGGGAGAT ATCAGTGGAG 
AAGTTGAGTT TAGATTTTGA TTCACCAATG AAGGTGGACA TCCGGCGGGA GGTGACAGAT 
ATAAGCACTC CGGAGAGGCA GCCGTCCACC GAGCCTCTCT CTGCTCCAGA CGTCACCACG 
CCTATCAGAA CACACTCATT GAGCAGTAAA CAGAAGAAGA CTTCTCTGAG CCTGCAGGAT 
TTCCGACTAA TTGCAGTTCT TGGCAGAGGC CATTTTGGAA AGGTGCTGCT GGCGGAATAT 
AAGAAGTCAG GGAGTATGTA TGCCATTAAA GCTCTAAAGA AAGGAGACAT TGTAGCCAGA 
GATGAAGTAG AAAGTTTGAT GTGTGAGAAG CGTATTTTCG AGGCAGTGAA CGGTGCACAA 
CACCCCTTCC TTGTCAACCT GTTTGCGTGT TTCCAGACTC CAGAACATGT GTGTTTTGTG 
ATGGAGTACA CAGCAGGCGG TGACCTCATG ATGCACATAC ATGCAGACGT CTTCACCGAG 
CCACGGGCTG TGTTTTATTC TGCTTGTGTA GTTTTAGGGC TTCAGTTTTT ACATGACAAC 
AAGATTGTGT ACCGAGATCT TAAATTAGAC AACTTGCTGC TTGACACAGA GGGTTATGTG 
AAGATTGCCG ACTTTGGATT GTGTAAAGAA GGTATGGGTT TTGGGGACCG GACCAGTACG 
TTCTGCGGGA CTCCAGAGTT TCTGGCTCCA GAGGTTTTGA CAGACACATC GTACACCCGT 
GCTGTGGACT GGTGGGGTCT CGGAGTGCTT ATATATGAAA TGTTGGTGGG AGAGTCCCCA 
TTCCCAGGTG ATGACGAAGA GGAAGTTTTT GACAGCATAG TGAATGATGA AGTTCGATAC 
CCCAGGTTCC TGTCCGCAGA GGCCATTGGC ATCATGAGGA GGTTACTAAG GAGAAATCCA 
GAAAGACGAC TGGGCTCCAG TGAGAAAGAT GCAGAAGATG TAAAGAAACA GCCTTTCTTC 
AGGAATATGG ACTGGGAGGC TCTTCTCCTC CGCAAACTCC CTCCTCCTTT TATTCCATCC 
ATCGGAGGAA AAGAAGACGT CAGCAACTTT GATGAGGAGT TCACGACAGA AGCGCCGACT 
CTCACTCCTC CCCGCGAGCC TCGAGTGCTT TCGCGCAAAG ATCAGGACAG TTTCCGGGAC 
TTCGACTATG TGTCTGACCT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_689331.3
CDS	177..3023
Translation	MASEEGRNTSLTEQHDDDTITQTETPSDPGGLSGLERLGLDHSSDYSDSSVQQRLDKERERIRREIRKELKIKEGAENLRRATTDKRNAQQVDSQLRCSNRRLDSLHAELQELDAHIVVKGTDDTHDDAPQSPGAESRSSAHKERIAALERQLNIELKVKQGAENMIPIYTNGSTKDKKMLQTAQQMLQDSKTKIDIIRMQIRKAVQAYEHNDDTQGKPDPCGMELRIEELRHHYRVEHAVAEGAKNVLRLLGASKVQDKKAMSEAQCRLSESSQRLDLLRDSLDRRLAELPEDHPKASVIKEELVLASSPAFSSQHSAPYLHNQYSTLSKPSPLTGRLQVTLLGCMGLLEVVPGRSRGSQVVLPCYSPGEGRSFMRSGKGLYSRSGSVSGKTPLKTDELSSDVSAVLKLDNTVVCQTAWKMVGEQGWDQTFIIELERSREMEIAVYWRDYRSLCALKFLKLEDFLDNQKHKVQLELEPQGLLLAEVTFFNPVIERVPRLKRQKNVFSKQQGKAFLRARQMNVDIGTFVRLLRNAIPTVNNTGTYSPNAHTLQTGEISVEKLSLDFDSPMKVDIRREVTDISTPERQPSTEPLSAPDVTTPIRTHSLSSKQKKTSLSLQDFRLIAVLGRGHFGKVLLAEYKKSGSMYAIKALKKGDIVARDEVESLMCEKRIFEAVNGAQHPFLVNLFACFQTPEHVCFVMEYTAGGDLMMHIHADVFTEPRAVFYSACVVLGLQFLHDNKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGFGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSEKDAEDVKKQPFFRNMDWEALLLRKLPPPFIPSIGGKEDVSNFDEEFTTEAPTLTPPREPRVLSRKDQDSFRDFDYVSDLC

Target ORF information:

RefSeq Version	XM_684239.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase N1a (pkn1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_684239.7

ORF Insert Sequence:

ATGGCCAGCG AGGAGGGAAG AAATACATCC CTCACAGAGC AGCACGACGA CGACACCATC 
ACACAGACTG AGACTCCGAG TGATCCCGGT GGGTTGTCTG GATTAGAGCG GCTTGGCTTG 
GACCACAGCT CTGATTACTC TGACTCGAGC GTACAACAGC GACTGGATAA AGAGCGCGAG 
CGAATCCGTC GGGAAATCCG CAAGGAGCTG AAGATTAAAG AGGGGGCAGA GAATCTCCGC 
AGAGCCACTA CAGATAAACG AAATGCTCAG CAGGTGGACA GCCAGTTGCG CTGCTCAAAT 
CGGCGTCTGG ACTCACTCCA TGCTGAATTA CAAGAACTGG ACGCTCATAT TGTGGTCAAA 
GGGACCGATG ATACACACGA TGACGCTCCT CAGTCTCCGG GAGCGGAGAG CAGGTCATCG 
GCACACAAGG AGAGAATTGC TGCTTTGGAA AGGCAGTTAA ACATCGAGCT CAAAGTCAAA 
CAGGGGGCAG AAAACATGAT CCCCATTTAT ACCAACGGTT CCACCAAGGA CAAGAAGATG 
TTGCAAACAG CTCAGCAGAT GCTACAGGAC AGCAAGACAA AGATTGACAT CATCCGCATG 
CAGATCCGGA AAGCAGTGCA GGCCTACGAA CACAACGATG ATACACAGGG AAAGCCTGAT 
CCATGTGGTA TGGAGCTACG CATTGAAGAG CTCAGACACC ATTACCGAGT AGAGCACGCC 
GTCGCTGAGG GAGCCAAAAA CGTCCTTAGA CTTCTTGGAG CCAGTAAAGT ACAAGACAAG 
AAGGCCATGT CAGAGGCTCA GTGTCGTCTG AGTGAATCGT CGCAAAGGTT GGACCTGCTG 
CGAGATTCTT TAGATCGACG GTTAGCAGAG CTTCCTGAGG ATCATCCCAA AGCTTCTGTT 
ATCAAAGAGG AGCTGGTTTT AGCATCCTCA CCAGCCTTCA GCTCTCAACA CAGCGCCCCC 
TACCTGCACA ACCAGTACAG CACTCTCTCC AAACCCTCAC CCCTCACTGG TCGGCTCCAG 
GTGACATTAT TGGGCTGTAT GGGTCTTCTG GAGGTGGTTC CCGGACGCTC TCGTGGTAGC 
CAGGTGGTTT TACCGTGTTA CAGTCCAGGA GAGGGACGAT CATTCATGCG CAGTGGAAAA 
GGCCTGTATA GTCGCAGTGG CAGCGTTAGT GGAAAAACAC CCCTCAAAAC CGACGAACTC 
TCCTCGGATG TGAGCGCAGT ATTGAAGCTG GACAACACTG TGGTGTGTCA GACGGCATGG 
AAGATGGTTG GAGAACAGGG CTGGGATCAG ACCTTCATCA TCGAGCTGGA GAGATCCAGA 
GAGATGGAGA TTGCCGTGTA CTGGAGAGAC TACCGCTCTC TGTGTGCGCT GAAATTTCTC 
AAGCTGGAGG ATTTCTTGGA CAACCAGAAA CACAAAGTAC AACTAGAGCT AGAACCACAG 
GGACTGCTGC TGGCAGAGGT TACTTTCTTT AACCCTGTGA TTGAGAGGGT TCCACGGCTA 
AAACGGCAGA AGAATGTTTT CTCCAAGCAG CAGGGTAAAG CATTTTTGCG AGCTCGACAG 
ATGAACGTTG ATATTGGCAC ATTCGTGAGG TTACTGAGGA ACGCCATACC AACCGTCAAC 
AACACAGGAA CCTACAGCCC TAACGCGCAC ACTCTACAGA CTGGGGAGAT ATCAGTGGAG 
AAGTTGAGTT TAGATTTTGA TTCACCAATG AAGGTGGACA TCCGGCGGGA GGTGACAGAT 
ATAAGCACTC CGGAGAGGCA GCCGTCCACC GAGCCTCTCT CTGCTCCAGA CGTCACCACG 
CCTATCAGAA CACACTCATT GAGCAGTAAA CAGAAGAAGA CTTCTCTGAG CCTGCAGGAT 
TTCCGACTAA TTGCAGTTCT TGGCAGAGGC CATTTTGGAA AGGTGCTGCT GGCGGAATAT 
AAGAAGTCAG GGAGTATGTA TGCCATTAAA GCTCTAAAGA AAGGAGACAT TGTAGCCAGA 
GATGAAGTAG AAAGTTTGAT GTGTGAGAAG CGTATTTTCG AGGCAGTGAA CGGTGCACAA 
CACCCCTTCC TTGTCAACCT GTTTGCGTGT TTCCAGACTC CAGAACATGT GTGTTTTGTG 
ATGGAGTACA CAGCAGGCGG TGACCTCATG ATGCACATAC ATGCAGACGT CTTCACCGAG 
CCACGGGCTG TGTTTTATTC TGCTTGTGTA GTTTTAGGGC TTCAGTTTTT ACATGACAAC 
AAGATTGTGT ACCGAGATCT TAAATTAGAC AACTTGCTGC TTGACACAGA GGGTTATGTG 
AAGATTGCCG ACTTTGGATT GTGTAAAGAA GGTATGGGTT TTGGGGACCG GACCAGTACG 
TTCTGCGGGA CTCCAGAGTT TCTGGCTCCA GAGGTTTTGA CAGACACATC GTACACCCGT 
GCTGTGGACT GGTGGGGTCT CGGAGTGCTT ATATATGAAA TGTTGGTGGG AGAGTCCCCA 
TTCCCAGGTG ATGACGAAGA GGAAGTTTTT GACAGCATAG TGAATGATGA AGTTCGATAC 
CCCAGGTTCC TGTCCGCAGA GGCCATTGGC ATCATGAGGA GGTTACTAAG GAGAAATCCA 
GAAAGACGAC TGGGCTCCAG TGAGAAAGAT GCAGAAGATG TAAAGAAACA GCCTTTCTTC 
AGGAATATGG ACTGGGAGGC TCTTCTCCTC CGCAAACTCC CTCCTCCTTT TATTCCATCC 
ATCGGAGGAA AAGAAGACGT CAGCAACTTT GATGAGGAGT TCACGACAGA AGCGCCGACT 
CTCACTCCTC CCCGCGAGCC TCGAGTGCTT TCGCGCAAAG ATCAGGACAG TTTCCGGGAC 
TTCGACTATG TGTCTGACCT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkn1a ( XM_005160051.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160108.1 Danio rerio protein kinase N1a (pkn1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa06097
Clone ID Related Accession (Same CDS sequence)	XM_005160051.4 , XM_005160051.3
Accession Version	XM_005160051.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase N1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160051.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG ACTCAATCCA AAGTGATCCC GGTGGGTTGT CTGGATTAGA GCGGCTTGGC 
TTGGACCACA GCTCTGATTA CTCTGACTCG AGCGTACAAC AGCGACTGGA TAAAGAGCGC 
GAGCGAATCC GTCGGGAAAT CCGCAAGGAG CTGAAGATTA AAGAGGGGGC AGAGAATCTC 
CGCAGAGCCA CTACAGATAA ACGAAATGCT CAGCAGGTGG ACAGCCAGTT GCGCTGCTCA 
AATCGGCGTC TGGACTCACT CCATGCTGAA TTACAAGAAC TGGACGCTCA TATTGTGGTC 
AAAGGGACCG ATGATACACA CGATGACGCT CCTCAGTCTC CGGGAGCGGA GAGCAGGTCA 
TCGGCACACA AGGAGAGAAT TGCTGCTTTG GAAAGGCAGT TAAACATCGA GCTCAAAGTC 
AAACAGGGGG CAGAAAACAT GATCCCCATT TATACCAACG GTTCCACCAA GGACAAGAAG 
ATGTTGCAAA CAGCTCAGCA GATGCTACAG GACAGCAAGA CAAAGATTGA CATCATCCGC 
ATGCAGATCC GGAAAGCAGT GCAGGCCTAC GAACACAACG ATGATACACA GGGAAAGCCT 
GATCCATGTG GTATGGAGCT ACGCATTGAA GAGCTCAGAC ACCATTACCG AGTAGAGCAC 
GCCGTCGCTG AGGGAGCCAA AAACGTCCTT AGACTTCTTG GAGCCAGTAA AGTACAAGAC 
AAGAAGGCCA TGTCAGAGGC TCAGTGTCGT CTGAGTGAAT CGTCGCAAAG GTTGGACCTG 
CTGCGAGATT CTTTAGATCG ACGGTTAGCA GAGCTTCCTG AGGATCATCC CAAAGCTTCT 
GTTATCAAAG AGGAGCTGGT TTTAGCATCC TCACCAGCCT TCAGCTCTCA ACACAGCGCC 
CCCTACCTGC ACAACCAGTA CAGCACTCTC TCCAAACCCT CACCCCTCAC TGGTCGGCTC 
CAGGTGACAT TATTGGGCTG TATGGGTCTT CTGGAGGTGG TTCCCGGACG CTCTCGTGGT 
AGCCAGGTGG TTTTACCGTG TTACAGTCCA GGAGAGGGAC GATCATTCAT GCGCAGTGGA 
AAAGGCCTGT ATAGTCGCAG TGGCAGCGTT AGTGGAAAAA CACCCCTCAA AACCGACGAA 
CTCTCCTCGG ATGTGAGCGC AGTATTGAAG CTGGACAACA CTGTGGTGTG TCAGACGGCA 
TGGAAGATGG TTGGAGAACA GGGCTGGGAT CAGACCTTCA TCATCGAGCT GGAGAGATCC 
AGAGAGATGG AGATTGCCGT GTACTGGAGA GACTACCGCT CTCTGTGTGC GCTGAAATTT 
CTCAAGCTGG AGGATTTCTT GGACAACCAG AAACACAAAG TACAACTAGA GCTAGAACCA 
CAGGGACTGC TGCTGGCAGA GGTTACTTTC TTTAACCCTG TGATTGAGAG GGTTCCACGG 
CTAAAACGGC AGAAGAATGT TTTCTCCAAG CAGCAGGGTA AAGCATTTTT GCGAGCTCGA 
CAGATGAACG TTGATATTGG CACATTCGTG AGGTTACTGA GGAACGCCAT ACCAACCGTC 
AACAACACAG GAACCTACAG CCCTAACGCG CACACTCTAC AGACTGGGGA GATATCAGTG 
GAGAAGTTGA GTTTAGATTT TGATTCACCA ATGAAGGTGG ACATCCGGCG GGAGGTGACA 
GATATAAGCA CTCCGGAGAG GCAGCCGTCC ACCGAGCCTC TCTCTGCTCC AGACGTCACC 
ACGCCTATCA GAACACACTC ATTGAGCAGT AAACAGAAGA AGACTTCTCT GAGCCTGCAG 
GATTTCCGAC TAATTGCAGT TCTTGGCAGA GGCCATTTTG GAAAGGTGCT GCTGGCGGAA 
TATAAGAAGT CAGGGAGTAT GTATGCCATT AAAGCTCTAA AGAAAGGAGA CATTGTAGCC 
AGAGATGAAG TAGAAAGTTT GATGTGTGAG AAGCGTATTT TCGAGGCAGT GAACGGTGCA 
CAACACCCCT TCCTTGTCAA CCTGTTTGCG TGTTTCCAGA CTCCAGAACA TGTGTGTTTT 
GTGATGGAGT ACACAGCAGG CGGTGACCTC ATGATGCACA TACATGCAGA CGTCTTCACC 
GAGCCACGGG CTGTGTTTTA TTCTGCTTGT GTAGTTTTAG GGCTTCAGTT TTTACATGAC 
AACAAGATTG TGTACCGAGA TCTTAAATTA GACAACTTGC TGCTTGACAC AGAGGGTTAT 
GTGAAGATTG CCGACTTTGG ATTGTGTAAA GAAGGTATGG GTTTTGGGGA CCGGACCAGT 
ACGTTCTGCG GGACTCCAGA GTTTCTGGCT CCAGAGGTTT TGACAGACAC ATCGTACACC 
CGTGCTGTGG ACTGGTGGGG TCTCGGAGTG CTTATATATG AAATGTTGGT GGGAGAGTCC 
CCATTCCCAG GTGATGACGA AGAGGAAGTT TTTGACAGCA TAGTGAATGA TGAAGTTCGA 
TACCCCAGGT TCCTGTCCGC AGAGGCCATT GGCATCATGA GGAGGTTACT AAGGAGAAAT 
CCAGAAAGAC GACTGGGCTC CAGTGAGAAA GATGCAGAAG ATGTAAAGAA ACAGCCTTTC 
TTCAGGAATA TGGACTGGGA GGCTCTTCTC CTCCGCAAAC TCCCTCCTCC TTTTATTCCA 
TCCATCGGAG GAAAAGAAGA CGTCAGCAAC TTTGATGAGG AGTTCACGAC AGAAGCGCCG 
ACTCTCACTC CTCCCCGCGA GCCTCGAGTG CTTTCGCGCA AAGATCAGGA CAGTTTCCGG 
GACTTCGACT ATGTGTCTGA CCTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160108.1
CDS	694..3483
Translation	MANDSIQSDPGGLSGLERLGLDHSSDYSDSSVQQRLDKERERIRREIRKELKIKEGAENLRRATTDKRNAQQVDSQLRCSNRRLDSLHAELQELDAHIVVKGTDDTHDDAPQSPGAESRSSAHKERIAALERQLNIELKVKQGAENMIPIYTNGSTKDKKMLQTAQQMLQDSKTKIDIIRMQIRKAVQAYEHNDDTQGKPDPCGMELRIEELRHHYRVEHAVAEGAKNVLRLLGASKVQDKKAMSEAQCRLSESSQRLDLLRDSLDRRLAELPEDHPKASVIKEELVLASSPAFSSQHSAPYLHNQYSTLSKPSPLTGRLQVTLLGCMGLLEVVPGRSRGSQVVLPCYSPGEGRSFMRSGKGLYSRSGSVSGKTPLKTDELSSDVSAVLKLDNTVVCQTAWKMVGEQGWDQTFIIELERSREMEIAVYWRDYRSLCALKFLKLEDFLDNQKHKVQLELEPQGLLLAEVTFFNPVIERVPRLKRQKNVFSKQQGKAFLRARQMNVDIGTFVRLLRNAIPTVNNTGTYSPNAHTLQTGEISVEKLSLDFDSPMKVDIRREVTDISTPERQPSTEPLSAPDVTTPIRTHSLSSKQKKTSLSLQDFRLIAVLGRGHFGKVLLAEYKKSGSMYAIKALKKGDIVARDEVESLMCEKRIFEAVNGAQHPFLVNLFACFQTPEHVCFVMEYTAGGDLMMHIHADVFTEPRAVFYSACVVLGLQFLHDNKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGFGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSEKDAEDVKKQPFFRNMDWEALLLRKLPPPFIPSIGGKEDVSNFDEEFTTEAPTLTPPREPRVLSRKDQDSFRDFDYVSDLC

Target ORF information:

RefSeq Version	XM_005160051.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase N1a (pkn1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160051.4

ORF Insert Sequence:

ATGGCAAATG ACTCAATCCA AAGTGATCCC GGTGGGTTGT CTGGATTAGA GCGGCTTGGC 
TTGGACCACA GCTCTGATTA CTCTGACTCG AGCGTACAAC AGCGACTGGA TAAAGAGCGC 
GAGCGAATCC GTCGGGAAAT CCGCAAGGAG CTGAAGATTA AAGAGGGGGC AGAGAATCTC 
CGCAGAGCCA CTACAGATAA ACGAAATGCT CAGCAGGTGG ACAGCCAGTT GCGCTGCTCA 
AATCGGCGTC TGGACTCACT CCATGCTGAA TTACAAGAAC TGGACGCTCA TATTGTGGTC 
AAAGGGACCG ATGATACACA CGATGACGCT CCTCAGTCTC CGGGAGCGGA GAGCAGGTCA 
TCGGCACACA AGGAGAGAAT TGCTGCTTTG GAAAGGCAGT TAAACATCGA GCTCAAAGTC 
AAACAGGGGG CAGAAAACAT GATCCCCATT TATACCAACG GTTCCACCAA GGACAAGAAG 
ATGTTGCAAA CAGCTCAGCA GATGCTACAG GACAGCAAGA CAAAGATTGA CATCATCCGC 
ATGCAGATCC GGAAAGCAGT GCAGGCCTAC GAACACAACG ATGATACACA GGGAAAGCCT 
GATCCATGTG GTATGGAGCT ACGCATTGAA GAGCTCAGAC ACCATTACCG AGTAGAGCAC 
GCCGTCGCTG AGGGAGCCAA AAACGTCCTT AGACTTCTTG GAGCCAGTAA AGTACAAGAC 
AAGAAGGCCA TGTCAGAGGC TCAGTGTCGT CTGAGTGAAT CGTCGCAAAG GTTGGACCTG 
CTGCGAGATT CTTTAGATCG ACGGTTAGCA GAGCTTCCTG AGGATCATCC CAAAGCTTCT 
GTTATCAAAG AGGAGCTGGT TTTAGCATCC TCACCAGCCT TCAGCTCTCA ACACAGCGCC 
CCCTACCTGC ACAACCAGTA CAGCACTCTC TCCAAACCCT CACCCCTCAC TGGTCGGCTC 
CAGGTGACAT TATTGGGCTG TATGGGTCTT CTGGAGGTGG TTCCCGGACG CTCTCGTGGT 
AGCCAGGTGG TTTTACCGTG TTACAGTCCA GGAGAGGGAC GATCATTCAT GCGCAGTGGA 
AAAGGCCTGT ATAGTCGCAG TGGCAGCGTT AGTGGAAAAA CACCCCTCAA AACCGACGAA 
CTCTCCTCGG ATGTGAGCGC AGTATTGAAG CTGGACAACA CTGTGGTGTG TCAGACGGCA 
TGGAAGATGG TTGGAGAACA GGGCTGGGAT CAGACCTTCA TCATCGAGCT GGAGAGATCC 
AGAGAGATGG AGATTGCCGT GTACTGGAGA GACTACCGCT CTCTGTGTGC GCTGAAATTT 
CTCAAGCTGG AGGATTTCTT GGACAACCAG AAACACAAAG TACAACTAGA GCTAGAACCA 
CAGGGACTGC TGCTGGCAGA GGTTACTTTC TTTAACCCTG TGATTGAGAG GGTTCCACGG 
CTAAAACGGC AGAAGAATGT TTTCTCCAAG CAGCAGGGTA AAGCATTTTT GCGAGCTCGA 
CAGATGAACG TTGATATTGG CACATTCGTG AGGTTACTGA GGAACGCCAT ACCAACCGTC 
AACAACACAG GAACCTACAG CCCTAACGCG CACACTCTAC AGACTGGGGA GATATCAGTG 
GAGAAGTTGA GTTTAGATTT TGATTCACCA ATGAAGGTGG ACATCCGGCG GGAGGTGACA 
GATATAAGCA CTCCGGAGAG GCAGCCGTCC ACCGAGCCTC TCTCTGCTCC AGACGTCACC 
ACGCCTATCA GAACACACTC ATTGAGCAGT AAACAGAAGA AGACTTCTCT GAGCCTGCAG 
GATTTCCGAC TAATTGCAGT TCTTGGCAGA GGCCATTTTG GAAAGGTGCT GCTGGCGGAA 
TATAAGAAGT CAGGGAGTAT GTATGCCATT AAAGCTCTAA AGAAAGGAGA CATTGTAGCC 
AGAGATGAAG TAGAAAGTTT GATGTGTGAG AAGCGTATTT TCGAGGCAGT GAACGGTGCA 
CAACACCCCT TCCTTGTCAA CCTGTTTGCG TGTTTCCAGA CTCCAGAACA TGTGTGTTTT 
GTGATGGAGT ACACAGCAGG CGGTGACCTC ATGATGCACA TACATGCAGA CGTCTTCACC 
GAGCCACGGG CTGTGTTTTA TTCTGCTTGT GTAGTTTTAG GGCTTCAGTT TTTACATGAC 
AACAAGATTG TGTACCGAGA TCTTAAATTA GACAACTTGC TGCTTGACAC AGAGGGTTAT 
GTGAAGATTG CCGACTTTGG ATTGTGTAAA GAAGGTATGG GTTTTGGGGA CCGGACCAGT 
ACGTTCTGCG GGACTCCAGA GTTTCTGGCT CCAGAGGTTT TGACAGACAC ATCGTACACC 
CGTGCTGTGG ACTGGTGGGG TCTCGGAGTG CTTATATATG AAATGTTGGT GGGAGAGTCC 
CCATTCCCAG GTGATGACGA AGAGGAAGTT TTTGACAGCA TAGTGAATGA TGAAGTTCGA 
TACCCCAGGT TCCTGTCCGC AGAGGCCATT GGCATCATGA GGAGGTTACT AAGGAGAAAT 
CCAGAAAGAC GACTGGGCTC CAGTGAGAAA GATGCAGAAG ATGTAAAGAA ACAGCCTTTC 
TTCAGGAATA TGGACTGGGA GGCTCTTCTC CTCCGCAAAC TCCCTCCTCC TTTTATTCCA 
TCCATCGGAG GAAAAGAAGA CGTCAGCAAC TTTGATGAGG AGTTCACGAC AGAAGCGCCG 
ACTCTCACTC CTCCCCGCGA GCCTCGAGTG CTTTCGCGCA AAGATCAGGA CAGTTTCCGG 
GACTTCGACT ATGTGTCTGA CCTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkn1a ( XM_005160051.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160108.1 Danio rerio protein kinase N1a (pkn1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa06097
Clone ID Related Accession (Same CDS sequence)	XM_005160051.4 , XM_005160051.3
Accession Version	XM_005160051.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase N1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160051.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG ACTCAATCCA AAGTGATCCC GGTGGGTTGT CTGGATTAGA GCGGCTTGGC 
TTGGACCACA GCTCTGATTA CTCTGACTCG AGCGTACAAC AGCGACTGGA TAAAGAGCGC 
GAGCGAATCC GTCGGGAAAT CCGCAAGGAG CTGAAGATTA AAGAGGGGGC AGAGAATCTC 
CGCAGAGCCA CTACAGATAA ACGAAATGCT CAGCAGGTGG ACAGCCAGTT GCGCTGCTCA 
AATCGGCGTC TGGACTCACT CCATGCTGAA TTACAAGAAC TGGACGCTCA TATTGTGGTC 
AAAGGGACCG ATGATACACA CGATGACGCT CCTCAGTCTC CGGGAGCGGA GAGCAGGTCA 
TCGGCACACA AGGAGAGAAT TGCTGCTTTG GAAAGGCAGT TAAACATCGA GCTCAAAGTC 
AAACAGGGGG CAGAAAACAT GATCCCCATT TATACCAACG GTTCCACCAA GGACAAGAAG 
ATGTTGCAAA CAGCTCAGCA GATGCTACAG GACAGCAAGA CAAAGATTGA CATCATCCGC 
ATGCAGATCC GGAAAGCAGT GCAGGCCTAC GAACACAACG ATGATACACA GGGAAAGCCT 
GATCCATGTG GTATGGAGCT ACGCATTGAA GAGCTCAGAC ACCATTACCG AGTAGAGCAC 
GCCGTCGCTG AGGGAGCCAA AAACGTCCTT AGACTTCTTG GAGCCAGTAA AGTACAAGAC 
AAGAAGGCCA TGTCAGAGGC TCAGTGTCGT CTGAGTGAAT CGTCGCAAAG GTTGGACCTG 
CTGCGAGATT CTTTAGATCG ACGGTTAGCA GAGCTTCCTG AGGATCATCC CAAAGCTTCT 
GTTATCAAAG AGGAGCTGGT TTTAGCATCC TCACCAGCCT TCAGCTCTCA ACACAGCGCC 
CCCTACCTGC ACAACCAGTA CAGCACTCTC TCCAAACCCT CACCCCTCAC TGGTCGGCTC 
CAGGTGACAT TATTGGGCTG TATGGGTCTT CTGGAGGTGG TTCCCGGACG CTCTCGTGGT 
AGCCAGGTGG TTTTACCGTG TTACAGTCCA GGAGAGGGAC GATCATTCAT GCGCAGTGGA 
AAAGGCCTGT ATAGTCGCAG TGGCAGCGTT AGTGGAAAAA CACCCCTCAA AACCGACGAA 
CTCTCCTCGG ATGTGAGCGC AGTATTGAAG CTGGACAACA CTGTGGTGTG TCAGACGGCA 
TGGAAGATGG TTGGAGAACA GGGCTGGGAT CAGACCTTCA TCATCGAGCT GGAGAGATCC 
AGAGAGATGG AGATTGCCGT GTACTGGAGA GACTACCGCT CTCTGTGTGC GCTGAAATTT 
CTCAAGCTGG AGGATTTCTT GGACAACCAG AAACACAAAG TACAACTAGA GCTAGAACCA 
CAGGGACTGC TGCTGGCAGA GGTTACTTTC TTTAACCCTG TGATTGAGAG GGTTCCACGG 
CTAAAACGGC AGAAGAATGT TTTCTCCAAG CAGCAGGGTA AAGCATTTTT GCGAGCTCGA 
CAGATGAACG TTGATATTGG CACATTCGTG AGGTTACTGA GGAACGCCAT ACCAACCGTC 
AACAACACAG GAACCTACAG CCCTAACGCG CACACTCTAC AGACTGGGGA GATATCAGTG 
GAGAAGTTGA GTTTAGATTT TGATTCACCA ATGAAGGTGG ACATCCGGCG GGAGGTGACA 
GATATAAGCA CTCCGGAGAG GCAGCCGTCC ACCGAGCCTC TCTCTGCTCC AGACGTCACC 
ACGCCTATCA GAACACACTC ATTGAGCAGT AAACAGAAGA AGACTTCTCT GAGCCTGCAG 
GATTTCCGAC TAATTGCAGT TCTTGGCAGA GGCCATTTTG GAAAGGTGCT GCTGGCGGAA 
TATAAGAAGT CAGGGAGTAT GTATGCCATT AAAGCTCTAA AGAAAGGAGA CATTGTAGCC 
AGAGATGAAG TAGAAAGTTT GATGTGTGAG AAGCGTATTT TCGAGGCAGT GAACGGTGCA 
CAACACCCCT TCCTTGTCAA CCTGTTTGCG TGTTTCCAGA CTCCAGAACA TGTGTGTTTT 
GTGATGGAGT ACACAGCAGG CGGTGACCTC ATGATGCACA TACATGCAGA CGTCTTCACC 
GAGCCACGGG CTGTGTTTTA TTCTGCTTGT GTAGTTTTAG GGCTTCAGTT TTTACATGAC 
AACAAGATTG TGTACCGAGA TCTTAAATTA GACAACTTGC TGCTTGACAC AGAGGGTTAT 
GTGAAGATTG CCGACTTTGG ATTGTGTAAA GAAGGTATGG GTTTTGGGGA CCGGACCAGT 
ACGTTCTGCG GGACTCCAGA GTTTCTGGCT CCAGAGGTTT TGACAGACAC ATCGTACACC 
CGTGCTGTGG ACTGGTGGGG TCTCGGAGTG CTTATATATG AAATGTTGGT GGGAGAGTCC 
CCATTCCCAG GTGATGACGA AGAGGAAGTT TTTGACAGCA TAGTGAATGA TGAAGTTCGA 
TACCCCAGGT TCCTGTCCGC AGAGGCCATT GGCATCATGA GGAGGTTACT AAGGAGAAAT 
CCAGAAAGAC GACTGGGCTC CAGTGAGAAA GATGCAGAAG ATGTAAAGAA ACAGCCTTTC 
TTCAGGAATA TGGACTGGGA GGCTCTTCTC CTCCGCAAAC TCCCTCCTCC TTTTATTCCA 
TCCATCGGAG GAAAAGAAGA CGTCAGCAAC TTTGATGAGG AGTTCACGAC AGAAGCGCCG 
ACTCTCACTC CTCCCCGCGA GCCTCGAGTG CTTTCGCGCA AAGATCAGGA CAGTTTCCGG 
GACTTCGACT ATGTGTCTGA CCTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160108.1
CDS	623..3412
Translation	MANDSIQSDPGGLSGLERLGLDHSSDYSDSSVQQRLDKERERIRREIRKELKIKEGAENLRRATTDKRNAQQVDSQLRCSNRRLDSLHAELQELDAHIVVKGTDDTHDDAPQSPGAESRSSAHKERIAALERQLNIELKVKQGAENMIPIYTNGSTKDKKMLQTAQQMLQDSKTKIDIIRMQIRKAVQAYEHNDDTQGKPDPCGMELRIEELRHHYRVEHAVAEGAKNVLRLLGASKVQDKKAMSEAQCRLSESSQRLDLLRDSLDRRLAELPEDHPKASVIKEELVLASSPAFSSQHSAPYLHNQYSTLSKPSPLTGRLQVTLLGCMGLLEVVPGRSRGSQVVLPCYSPGEGRSFMRSGKGLYSRSGSVSGKTPLKTDELSSDVSAVLKLDNTVVCQTAWKMVGEQGWDQTFIIELERSREMEIAVYWRDYRSLCALKFLKLEDFLDNQKHKVQLELEPQGLLLAEVTFFNPVIERVPRLKRQKNVFSKQQGKAFLRARQMNVDIGTFVRLLRNAIPTVNNTGTYSPNAHTLQTGEISVEKLSLDFDSPMKVDIRREVTDISTPERQPSTEPLSAPDVTTPIRTHSLSSKQKKTSLSLQDFRLIAVLGRGHFGKVLLAEYKKSGSMYAIKALKKGDIVARDEVESLMCEKRIFEAVNGAQHPFLVNLFACFQTPEHVCFVMEYTAGGDLMMHIHADVFTEPRAVFYSACVVLGLQFLHDNKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGFGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSEKDAEDVKKQPFFRNMDWEALLLRKLPPPFIPSIGGKEDVSNFDEEFTTEAPTLTPPREPRVLSRKDQDSFRDFDYVSDLC

Target ORF information:

RefSeq Version	XM_005160051.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein kinase N1a (pkn1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160051.3

ORF Insert Sequence:

ATGGCAAATG ACTCAATCCA AAGTGATCCC GGTGGGTTGT CTGGATTAGA GCGGCTTGGC 
TTGGACCACA GCTCTGATTA CTCTGACTCG AGCGTACAAC AGCGACTGGA TAAAGAGCGC 
GAGCGAATCC GTCGGGAAAT CCGCAAGGAG CTGAAGATTA AAGAGGGGGC AGAGAATCTC 
CGCAGAGCCA CTACAGATAA ACGAAATGCT CAGCAGGTGG ACAGCCAGTT GCGCTGCTCA 
AATCGGCGTC TGGACTCACT CCATGCTGAA TTACAAGAAC TGGACGCTCA TATTGTGGTC 
AAAGGGACCG ATGATACACA CGATGACGCT CCTCAGTCTC CGGGAGCGGA GAGCAGGTCA 
TCGGCACACA AGGAGAGAAT TGCTGCTTTG GAAAGGCAGT TAAACATCGA GCTCAAAGTC 
AAACAGGGGG CAGAAAACAT GATCCCCATT TATACCAACG GTTCCACCAA GGACAAGAAG 
ATGTTGCAAA CAGCTCAGCA GATGCTACAG GACAGCAAGA CAAAGATTGA CATCATCCGC 
ATGCAGATCC GGAAAGCAGT GCAGGCCTAC GAACACAACG ATGATACACA GGGAAAGCCT 
GATCCATGTG GTATGGAGCT ACGCATTGAA GAGCTCAGAC ACCATTACCG AGTAGAGCAC 
GCCGTCGCTG AGGGAGCCAA AAACGTCCTT AGACTTCTTG GAGCCAGTAA AGTACAAGAC 
AAGAAGGCCA TGTCAGAGGC TCAGTGTCGT CTGAGTGAAT CGTCGCAAAG GTTGGACCTG 
CTGCGAGATT CTTTAGATCG ACGGTTAGCA GAGCTTCCTG AGGATCATCC CAAAGCTTCT 
GTTATCAAAG AGGAGCTGGT TTTAGCATCC TCACCAGCCT TCAGCTCTCA ACACAGCGCC 
CCCTACCTGC ACAACCAGTA CAGCACTCTC TCCAAACCCT CACCCCTCAC TGGTCGGCTC 
CAGGTGACAT TATTGGGCTG TATGGGTCTT CTGGAGGTGG TTCCCGGACG CTCTCGTGGT 
AGCCAGGTGG TTTTACCGTG TTACAGTCCA GGAGAGGGAC GATCATTCAT GCGCAGTGGA 
AAAGGCCTGT ATAGTCGCAG TGGCAGCGTT AGTGGAAAAA CACCCCTCAA AACCGACGAA 
CTCTCCTCGG ATGTGAGCGC AGTATTGAAG CTGGACAACA CTGTGGTGTG TCAGACGGCA 
TGGAAGATGG TTGGAGAACA GGGCTGGGAT CAGACCTTCA TCATCGAGCT GGAGAGATCC 
AGAGAGATGG AGATTGCCGT GTACTGGAGA GACTACCGCT CTCTGTGTGC GCTGAAATTT 
CTCAAGCTGG AGGATTTCTT GGACAACCAG AAACACAAAG TACAACTAGA GCTAGAACCA 
CAGGGACTGC TGCTGGCAGA GGTTACTTTC TTTAACCCTG TGATTGAGAG GGTTCCACGG 
CTAAAACGGC AGAAGAATGT TTTCTCCAAG CAGCAGGGTA AAGCATTTTT GCGAGCTCGA 
CAGATGAACG TTGATATTGG CACATTCGTG AGGTTACTGA GGAACGCCAT ACCAACCGTC 
AACAACACAG GAACCTACAG CCCTAACGCG CACACTCTAC AGACTGGGGA GATATCAGTG 
GAGAAGTTGA GTTTAGATTT TGATTCACCA ATGAAGGTGG ACATCCGGCG GGAGGTGACA 
GATATAAGCA CTCCGGAGAG GCAGCCGTCC ACCGAGCCTC TCTCTGCTCC AGACGTCACC 
ACGCCTATCA GAACACACTC ATTGAGCAGT AAACAGAAGA AGACTTCTCT GAGCCTGCAG 
GATTTCCGAC TAATTGCAGT TCTTGGCAGA GGCCATTTTG GAAAGGTGCT GCTGGCGGAA 
TATAAGAAGT CAGGGAGTAT GTATGCCATT AAAGCTCTAA AGAAAGGAGA CATTGTAGCC 
AGAGATGAAG TAGAAAGTTT GATGTGTGAG AAGCGTATTT TCGAGGCAGT GAACGGTGCA 
CAACACCCCT TCCTTGTCAA CCTGTTTGCG TGTTTCCAGA CTCCAGAACA TGTGTGTTTT 
GTGATGGAGT ACACAGCAGG CGGTGACCTC ATGATGCACA TACATGCAGA CGTCTTCACC 
GAGCCACGGG CTGTGTTTTA TTCTGCTTGT GTAGTTTTAG GGCTTCAGTT TTTACATGAC 
AACAAGATTG TGTACCGAGA TCTTAAATTA GACAACTTGC TGCTTGACAC AGAGGGTTAT 
GTGAAGATTG CCGACTTTGG ATTGTGTAAA GAAGGTATGG GTTTTGGGGA CCGGACCAGT 
ACGTTCTGCG GGACTCCAGA GTTTCTGGCT CCAGAGGTTT TGACAGACAC ATCGTACACC 
CGTGCTGTGG ACTGGTGGGG TCTCGGAGTG CTTATATATG AAATGTTGGT GGGAGAGTCC 
CCATTCCCAG GTGATGACGA AGAGGAAGTT TTTGACAGCA TAGTGAATGA TGAAGTTCGA 
TACCCCAGGT TCCTGTCCGC AGAGGCCATT GGCATCATGA GGAGGTTACT AAGGAGAAAT 
CCAGAAAGAC GACTGGGCTC CAGTGAGAAA GATGCAGAAG ATGTAAAGAA ACAGCCTTTC 
TTCAGGAATA TGGACTGGGA GGCTCTTCTC CTCCGCAAAC TCCCTCCTCC TTTTATTCCA 
TCCATCGGAG GAAAAGAAGA CGTCAGCAAC TTTGATGAGG AGTTCACGAC AGAAGCGCCG 
ACTCTCACTC CTCCCCGCGA GCCTCGAGTG CTTTCGCGCA AAGATCAGGA CAGTTTCCGG 
GACTTCGACT ATGTGTCTGA CCTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkn1a ( XM_005160050.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160107.3 Danio rerio protein kinase N1a (pkn1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147438
Clone ID Related Accession (Same CDS sequence)	XM_005160050.4
Accession Version	XM_005160050.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase N1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160050.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTCT CTCTCTTTTC CTCCGTCTCC CTCTGTCCTT CTGTTTCTAT GCTACTGGAT 
GTCGGTGTCA TCGCCGGAGA GAGGAGAATG TGTTGGTCTG GTGAATGGGA TCATTGGGAA 
TTTCTGGATA TCCAGAGTGA TCCCGGTGGG TTGTCTGGAT TAGAGCGGCT TGGCTTGGAC 
CACAGCTCTG ATTACTCTGA CTCGAGCGTA CAACAGCGAC TGGATAAAGA GCGCGAGCGA 
ATCCGTCGGG AAATCCGCAA GGAGCTGAAG ATTAAAGAGG GGGCAGAGAA TCTCCGCAGA 
GCCACTACAG ATAAACGAAA TGCTCAGCAG GTGGACAGCC AGTTGCGCTG CTCAAATCGG 
CGTCTGGACT CACTCCATGC TGAATTACAA GAACTGGACG CTCATATTGT GGTCAAAGGG 
ACCGATGATA CACACGATGA CGCTCCTCAG TCTCCGGGAG CGGAGAGCAG GTCATCGGCA 
CACAAGGAGA GAATTGCTGC TTTGGAAAGG CAGTTAAACA TCGAGCTCAA AGTCAAACAG 
GGGGCAGAAA ACATGATCCC CATTTATACC AACGGTTCCA CCAAGGACAA GAAGATGTTG 
CAAACAGCTC AGCAGATGCT ACAGGACAGC AAGACAAAGA TTGACATCAT CCGCATGCAG 
ATCCGGAAAG CAGTGCAGGC CTACGAACAC AACGATGATA CACAGGGAAA GCCTGATCCA 
TGTGGTATGG AGCTACGCAT TGAAGAGCTC AGACACCATT ACCGAGTAGA GCACGCCGTC 
GCTGAGGGAG CCAAAAACGT CCTTAGACTT CTTGGAGCCA GTAAAGTACA AGACAAGAAG 
GCCATGTCAG AGGCTCAGTG TCGTCTGAGT GAATCGTCGC AAAGGTTGGA CCTGCTGCGA 
GATTCTTTAG ATCGACGGTT AGCAGAGCTT CCTGAGGATC ATCCCAAAGC TTCTGTTATC 
AAAGAGGAGC TGGTTTTAGC ATCCTCACCA GCCTTCAGCT CTCAACACAG CGCCCCCTAC 
CTGCACAACC AGTACAGCAC TCTCTCCAAA CCCTCACCCC TCACTGGTCG GCTCCAGGTG 
ACATTATTGG GCTGTATGGG TCTTCTGGAG GTGGTTCCCG GACGCTCTCG TGGTAGCCAG 
GTGGTTTTAC CGTGTTACAG TCCAGGAGAG GGACGATCAT TCATGCGCAG TGGAAAAGGC 
CTGTATAGTC GCAGTGGCAG CGTTAGTGGA AAAACACCCC TCAAAACCGA CGAACTCTCC 
TCGGATGTGA GCGCAGTATT GAAGCTGGAC AACACTGTGG TGTGTCAGAC GGCATGGAAG 
ATGGTTGGAG AACAGGGCTG GGATCAGACC TTCATCATCG AGCTGGAGAG ATCCAGAGAG 
ATGGAGATTG CCGTGTACTG GAGAGACTAC CGCTCTCTGT GTGCGCTGAA ATTTCTCAAG 
CTGGAGGATT TCTTGGACAA CCAGAAACAC AAAGTACAAC TAGAGCTAGA ACCACAGGGA 
CTGCTGCTGG CAGAGGTTAC TTTCTTTAAC CCTGTGATTG AGAGGGTTCC ACGGCTAAAA 
CGGCAGAAGA ATGTTTTCTC CAAGCAGCAG GGTAAAGCAT TTTTGCGAGC TCGACAGATG 
AACGTTGATA TTGGCACATT CGTGAGGTTA CTGAGGAACG CCATACCAAC CGTCAACAAC 
ACAGGAACCT ACAGCCCTAA CGCGCACACT CTACAGACTG GGGAGATATC AGTGGAGAAG 
TTGAGTTTAG ATTTTGATTC ACCAATGAAG GTGGACATCC GGCGGGAGGT GACAGATATA 
AGCACTCCGG AGAGGCAGCC GTCCACCGAG CCTCTCTCTG CTCCAGACGT CACCACGCCT 
ATCAGAACAC ACTCATTGAG CAGTAAACAG AAGAAGACTT CTCTGAGCCT GCAGGATTTC 
CGACTAATTG CAGTTCTTGG CAGAGGCCAT TTTGGAAAGG TGCTGCTGGC GGAATATAAG 
AAGTCAGGGA GTATGTATGC CATTAAAGCT CTAAAGAAAG GAGACATTGT AGCCAGAGAT 
GAAGTAGAAA GTTTGATGTG TGAGAAGCGT ATTTTCGAGG CAGTGAACGG TGCACAACAC 
CCCTTCCTTG TCAACCTGTT TGCGTGTTTC CAGACTCCAG AACATGTGTG TTTTGTGATG 
GAGTACACAG CAGGCGGTGA CCTCATGATG CACATACATG CAGACGTCTT CACCGAGCCA 
CGGGCTGTGT TTTATTCTGC TTGTGTAGTT TTAGGGCTTC AGTTTTTACA TGACAACAAG 
ATTGTGTACC GAGATCTTAA ATTAGACAAC TTGCTGCTTG ACACAGAGGG TTATGTGAAG 
ATTGCCGACT TTGGATTGTG TAAAGAAGGT ATGGGTTTTG GGGACCGGAC CAGTACGTTC 
TGCGGGACTC CAGAGTTTCT GGCTCCAGAG GTTTTGACAG ACACATCGTA CACCCGTGCT 
GTGGACTGGT GGGGTCTCGG AGTGCTTATA TATGAAATGT TGGTGGGAGA GTCCCCATTC 
CCAGGTGATG ACGAAGAGGA AGTTTTTGAC AGCATAGTGA ATGATGAAGT TCGATACCCC 
AGGTTCCTGT CCGCAGAGGC CATTGGCATC ATGAGGAGGT TACTAAGGAG AAATCCAGAA 
AGACGACTGG GCTCCAGTGA GAAAGATGCA GAAGATGTAA AGAAACAGCC TTTCTTCAGG 
AATATGGACT GGGAGGCTCT TCTCCTCCGC AAACTCCCTC CTCCTTTTAT TCCATCCATC 
GGAGGAAAAG AAGACGTCAG CAACTTTGAT GAGGAGTTCA CGACAGAAGC GCCGACTCTC 
ACTCCTCCCC GCGAGCCTCG AGTGCTTTCG CGCAAAGATC AGGACAGTTT CCGGGACTTC 
GACTATGTGT CTGACCTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160107.3
CDS	1..2904
Translation	MSFSLFSSVSLCPSVSMLLDVGVIAGERRMCWSGEWDHWEFLDIQSDPGGLSGLERLGLDHSSDYSDSSVQQRLDKERERIRREIRKELKIKEGAENLRRATTDKRNAQQVDSQLRCSNRRLDSLHAELQELDAHIVVKGTDDTHDDAPQSPGAESRSSAHKERIAALERQLNIELKVKQGAENMIPIYTNGSTKDKKMLQTAQQMLQDSKTKIDIIRMQIRKAVQAYEHNDDTQGKPDPCGMELRIEELRHHYRVEHAVAEGAKNVLRLLGASKVQDKKAMSEAQCRLSESSQRLDLLRDSLDRRLAELPEDHPKASVIKEELVLASSPAFSSQHSAPYLHNQYSTLSKPSPLTGRLQVTLLGCMGLLEVVPGRSRGSQVVLPCYSPGEGRSFMRSGKGLYSRSGSVSGKTPLKTDELSSDVSAVLKLDNTVVCQTAWKMVGEQGWDQTFIIELERSREMEIAVYWRDYRSLCALKFLKLEDFLDNQKHKVQLELEPQGLLLAEVTFFNPVIERVPRLKRQKNVFSKQQGKAFLRARQMNVDIGTFVRLLRNAIPTVNNTGTYSPNAHTLQTGEISVEKLSLDFDSPMKVDIRREVTDISTPERQPSTEPLSAPDVTTPIRTHSLSSKQKKTSLSLQDFRLIAVLGRGHFGKVLLAEYKKSGSMYAIKALKKGDIVARDEVESLMCEKRIFEAVNGAQHPFLVNLFACFQTPEHVCFVMEYTAGGDLMMHIHADVFTEPRAVFYSACVVLGLQFLHDNKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGFGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSEKDAEDVKKQPFFRNMDWEALLLRKLPPPFIPSIGGKEDVSNFDEEFTTEAPTLTPPREPRVLSRKDQDSFRDFDYVSDLC

Target ORF information:

RefSeq Version	XM_005160050.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase N1a (pkn1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160050.4

ORF Insert Sequence:

ATGTCTTTCT CTCTCTTTTC CTCCGTCTCC CTCTGTCCTT CTGTTTCTAT GCTACTGGAT 
GTCGGTGTCA TCGCCGGAGA GAGGAGAATG TGTTGGTCTG GTGAATGGGA TCATTGGGAA 
TTTCTGGATA TCCAGAGTGA TCCCGGTGGG TTGTCTGGAT TAGAGCGGCT TGGCTTGGAC 
CACAGCTCTG ATTACTCTGA CTCGAGCGTA CAACAGCGAC TGGATAAAGA GCGCGAGCGA 
ATCCGTCGGG AAATCCGCAA GGAGCTGAAG ATTAAAGAGG GGGCAGAGAA TCTCCGCAGA 
GCCACTACAG ATAAACGAAA TGCTCAGCAG GTGGACAGCC AGTTGCGCTG CTCAAATCGG 
CGTCTGGACT CACTCCATGC TGAATTACAA GAACTGGACG CTCATATTGT GGTCAAAGGG 
ACCGATGATA CACACGATGA CGCTCCTCAG TCTCCGGGAG CGGAGAGCAG GTCATCGGCA 
CACAAGGAGA GAATTGCTGC TTTGGAAAGG CAGTTAAACA TCGAGCTCAA AGTCAAACAG 
GGGGCAGAAA ACATGATCCC CATTTATACC AACGGTTCCA CCAAGGACAA GAAGATGTTG 
CAAACAGCTC AGCAGATGCT ACAGGACAGC AAGACAAAGA TTGACATCAT CCGCATGCAG 
ATCCGGAAAG CAGTGCAGGC CTACGAACAC AACGATGATA CACAGGGAAA GCCTGATCCA 
TGTGGTATGG AGCTACGCAT TGAAGAGCTC AGACACCATT ACCGAGTAGA GCACGCCGTC 
GCTGAGGGAG CCAAAAACGT CCTTAGACTT CTTGGAGCCA GTAAAGTACA AGACAAGAAG 
GCCATGTCAG AGGCTCAGTG TCGTCTGAGT GAATCGTCGC AAAGGTTGGA CCTGCTGCGA 
GATTCTTTAG ATCGACGGTT AGCAGAGCTT CCTGAGGATC ATCCCAAAGC TTCTGTTATC 
AAAGAGGAGC TGGTTTTAGC ATCCTCACCA GCCTTCAGCT CTCAACACAG CGCCCCCTAC 
CTGCACAACC AGTACAGCAC TCTCTCCAAA CCCTCACCCC TCACTGGTCG GCTCCAGGTG 
ACATTATTGG GCTGTATGGG TCTTCTGGAG GTGGTTCCCG GACGCTCTCG TGGTAGCCAG 
GTGGTTTTAC CGTGTTACAG TCCAGGAGAG GGACGATCAT TCATGCGCAG TGGAAAAGGC 
CTGTATAGTC GCAGTGGCAG CGTTAGTGGA AAAACACCCC TCAAAACCGA CGAACTCTCC 
TCGGATGTGA GCGCAGTATT GAAGCTGGAC AACACTGTGG TGTGTCAGAC GGCATGGAAG 
ATGGTTGGAG AACAGGGCTG GGATCAGACC TTCATCATCG AGCTGGAGAG ATCCAGAGAG 
ATGGAGATTG CCGTGTACTG GAGAGACTAC CGCTCTCTGT GTGCGCTGAA ATTTCTCAAG 
CTGGAGGATT TCTTGGACAA CCAGAAACAC AAAGTACAAC TAGAGCTAGA ACCACAGGGA 
CTGCTGCTGG CAGAGGTTAC TTTCTTTAAC CCTGTGATTG AGAGGGTTCC ACGGCTAAAA 
CGGCAGAAGA ATGTTTTCTC CAAGCAGCAG GGTAAAGCAT TTTTGCGAGC TCGACAGATG 
AACGTTGATA TTGGCACATT CGTGAGGTTA CTGAGGAACG CCATACCAAC CGTCAACAAC 
ACAGGAACCT ACAGCCCTAA CGCGCACACT CTACAGACTG GGGAGATATC AGTGGAGAAG 
TTGAGTTTAG ATTTTGATTC ACCAATGAAG GTGGACATCC GGCGGGAGGT GACAGATATA 
AGCACTCCGG AGAGGCAGCC GTCCACCGAG CCTCTCTCTG CTCCAGACGT CACCACGCCT 
ATCAGAACAC ACTCATTGAG CAGTAAACAG AAGAAGACTT CTCTGAGCCT GCAGGATTTC 
CGACTAATTG CAGTTCTTGG CAGAGGCCAT TTTGGAAAGG TGCTGCTGGC GGAATATAAG 
AAGTCAGGGA GTATGTATGC CATTAAAGCT CTAAAGAAAG GAGACATTGT AGCCAGAGAT 
GAAGTAGAAA GTTTGATGTG TGAGAAGCGT ATTTTCGAGG CAGTGAACGG TGCACAACAC 
CCCTTCCTTG TCAACCTGTT TGCGTGTTTC CAGACTCCAG AACATGTGTG TTTTGTGATG 
GAGTACACAG CAGGCGGTGA CCTCATGATG CACATACATG CAGACGTCTT CACCGAGCCA 
CGGGCTGTGT TTTATTCTGC TTGTGTAGTT TTAGGGCTTC AGTTTTTACA TGACAACAAG 
ATTGTGTACC GAGATCTTAA ATTAGACAAC TTGCTGCTTG ACACAGAGGG TTATGTGAAG 
ATTGCCGACT TTGGATTGTG TAAAGAAGGT ATGGGTTTTG GGGACCGGAC CAGTACGTTC 
TGCGGGACTC CAGAGTTTCT GGCTCCAGAG GTTTTGACAG ACACATCGTA CACCCGTGCT 
GTGGACTGGT GGGGTCTCGG AGTGCTTATA TATGAAATGT TGGTGGGAGA GTCCCCATTC 
CCAGGTGATG ACGAAGAGGA AGTTTTTGAC AGCATAGTGA ATGATGAAGT TCGATACCCC 
AGGTTCCTGT CCGCAGAGGC CATTGGCATC ATGAGGAGGT TACTAAGGAG AAATCCAGAA 
AGACGACTGG GCTCCAGTGA GAAAGATGCA GAAGATGTAA AGAAACAGCC TTTCTTCAGG 
AATATGGACT GGGAGGCTCT TCTCCTCCGC AAACTCCCTC CTCCTTTTAT TCCATCCATC 
GGAGGAAAAG AAGACGTCAG CAACTTTGAT GAGGAGTTCA CGACAGAAGC GCCGACTCTC 
ACTCCTCCCC GCGAGCCTCG AGTGCTTTCG CGCAAAGATC AGGACAGTTT CCGGGACTTC 
GACTATGTGT CTGACCTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkn1a ( XM_005160050.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160107.2 Danio rerio protein kinase N1a (pkn1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa43355
Clone ID Related Accession (Same CDS sequence)	XM_005160050.3
Accession Version	XM_005160050.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase N1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160050.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACTGG ATGTCGGTGT CATCGCCGGA GAGAGGAGAA TGTGTTGGTC TGGTGAATGG 
GATCATTGGG AATTTCTGGA TATCCAGAGT GATCCCGGTG GGTTGTCTGG ATTAGAGCGG 
CTTGGCTTGG ACCACAGCTC TGATTACTCT GACTCGAGCG TACAACAGCG ACTGGATAAA 
GAGCGCGAGC GAATCCGTCG GGAAATCCGC AAGGAGCTGA AGATTAAAGA GGGGGCAGAG 
AATCTCCGCA GAGCCACTAC AGATAAACGA AATGCTCAGC AGGTGGACAG CCAGTTGCGC 
TGCTCAAATC GGCGTCTGGA CTCACTCCAT GCTGAATTAC AAGAACTGGA CGCTCATATT 
GTGGTCAAAG GGACCGATGA TACACACGAT GACGCTCCTC AGTCTCCGGG AGCGGAGAGC 
AGGTCATCGG CACACAAGGA GAGAATTGCT GCTTTGGAAA GGCAGTTAAA CATCGAGCTC 
AAAGTCAAAC AGGGGGCAGA AAACATGATC CCCATTTATA CCAACGGTTC CACCAAGGAC 
AAGAAGATGT TGCAAACAGC TCAGCAGATG CTACAGGACA GCAAGACAAA GATTGACATC 
ATCCGCATGC AGATCCGGAA AGCAGTGCAG GCCTACGAAC ACAACGATGA TACACAGGGA 
AAGCCTGATC CATGTGGTAT GGAGCTACGC ATTGAAGAGC TCAGACACCA TTACCGAGTA 
GAGCACGCCG TCGCTGAGGG AGCCAAAAAC GTCCTTAGAC TTCTTGGAGC CAGTAAAGTA 
CAAGACAAGA AGGCCATGTC AGAGGCTCAG TGTCGTCTGA GTGAATCGTC GCAAAGGTTG 
GACCTGCTGC GAGATTCTTT AGATCGACGG TTAGCAGAGC TTCCTGAGGA TCATCCCAAA 
GCTTCTGTTA TCAAAGAGGA GCTGGTTTTA GCATCCTCAC CAGCCTTCAG CTCTCAACAC 
AGCGCCCCCT ACCTGCACAA CCAGTACAGC ACTCTCTCCA AACCCTCACC CCTCACTGGT 
CGGCTCCAGG TGACATTATT GGGCTGTATG GGTCTTCTGG AGGTGGTTCC CGGACGCTCT 
CGTGGTAGCC AGGTGGTTTT ACCGTGTTAC AGTCCAGGAG AGGGACGATC ATTCATGCGC 
AGTGGAAAAG GCCTGTATAG TCGCAGTGGC AGCGTTAGTG GAAAAACACC CCTCAAAACC 
GACGAACTCT CCTCGGATGT GAGCGCAGTA TTGAAGCTGG ACAACACTGT GGTGTGTCAG 
ACGGCATGGA AGATGGTTGG AGAACAGGGC TGGGATCAGA CCTTCATCAT CGAGCTGGAG 
AGATCCAGAG AGATGGAGAT TGCCGTGTAC TGGAGAGACT ACCGCTCTCT GTGTGCGCTG 
AAATTTCTCA AGCTGGAGGA TTTCTTGGAC AACCAGAAAC ACAAAGTACA ACTAGAGCTA 
GAACCACAGG GACTGCTGCT GGCAGAGGTT ACTTTCTTTA ACCCTGTGAT TGAGAGGGTT 
CCACGGCTAA AACGGCAGAA GAATGTTTTC TCCAAGCAGC AGGGTAAAGC ATTTTTGCGA 
GCTCGACAGA TGAACGTTGA TATTGGCACA TTCGTGAGGT TACTGAGGAA CGCCATACCA 
ACCGTCAACA ACACAGGAAC CTACAGCCCT AACGCGCACA CTCTACAGAC TGGGGAGATA 
TCAGTGGAGA AGTTGAGTTT AGATTTTGAT TCACCAATGA AGGTGGACAT CCGGCGGGAG 
GTGACAGATA TAAGCACTCC GGAGAGGCAG CCGTCCACCG AGCCTCTCTC TGCTCCAGAC 
GTCACCACGC CTATCAGAAC ACACTCATTG AGCAGTAAAC AGAAGAAGAC TTCTCTGAGC 
CTGCAGGATT TCCGACTAAT TGCAGTTCTT GGCAGAGGCC ATTTTGGAAA GGTGCTGCTG 
GCGGAATATA AGAAGTCAGG GAGTATGTAT GCCATTAAAG CTCTAAAGAA AGGAGACATT 
GTAGCCAGAG ATGAAGTAGA AAGTTTGATG TGTGAGAAGC GTATTTTCGA GGCAGTGAAC 
GGTGCACAAC ACCCCTTCCT TGTCAACCTG TTTGCGTGTT TCCAGACTCC AGAACATGTG 
TGTTTTGTGA TGGAGTACAC AGCAGGCGGT GACCTCATGA TGCACATACA TGCAGACGTC 
TTCACCGAGC CACGGGCTGT GTTTTATTCT GCTTGTGTAG TTTTAGGGCT TCAGTTTTTA 
CATGACAACA AGATTGTGTA CCGAGATCTT AAATTAGACA ACTTGCTGCT TGACACAGAG 
GGTTATGTGA AGATTGCCGA CTTTGGATTG TGTAAAGAAG GTATGGGTTT TGGGGACCGG 
ACCAGTACGT TCTGCGGGAC TCCAGAGTTT CTGGCTCCAG AGGTTTTGAC AGACACATCG 
TACACCCGTG CTGTGGACTG GTGGGGTCTC GGAGTGCTTA TATATGAAAT GTTGGTGGGA 
GAGTCCCCAT TCCCAGGTGA TGACGAAGAG GAAGTTTTTG ACAGCATAGT GAATGATGAA 
GTTCGATACC CCAGGTTCCT GTCCGCAGAG GCCATTGGCA TCATGAGGAG GTTACTAAGG 
AGAAATCCAG AAAGACGACT GGGCTCCAGT GAGAAAGATG CAGAAGATGT AAAGAAACAG 
CCTTTCTTCA GGAATATGGA CTGGGAGGCT CTTCTCCTCC GCAAACTCCC TCCTCCTTTT 
ATTCCATCCA TCGGAGGAAA AGAAGACGTC AGCAACTTTG ATGAGGAGTT CACGACAGAA 
GCGCCGACTC TCACTCCTCC CCGCGAGCCT CGAGTGCTTT CGCGCAAAGA TCAGGACAGT 
TTCCGGGACT TCGACTATGT GTCTGACCTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160107.2
CDS	21..2876
Translation	MLLDVGVIAGERRMCWSGEWDHWEFLDIQSDPGGLSGLERLGLDHSSDYSDSSVQQRLDKERERIRREIRKELKIKEGAENLRRATTDKRNAQQVDSQLRCSNRRLDSLHAELQELDAHIVVKGTDDTHDDAPQSPGAESRSSAHKERIAALERQLNIELKVKQGAENMIPIYTNGSTKDKKMLQTAQQMLQDSKTKIDIIRMQIRKAVQAYEHNDDTQGKPDPCGMELRIEELRHHYRVEHAVAEGAKNVLRLLGASKVQDKKAMSEAQCRLSESSQRLDLLRDSLDRRLAELPEDHPKASVIKEELVLASSPAFSSQHSAPYLHNQYSTLSKPSPLTGRLQVTLLGCMGLLEVVPGRSRGSQVVLPCYSPGEGRSFMRSGKGLYSRSGSVSGKTPLKTDELSSDVSAVLKLDNTVVCQTAWKMVGEQGWDQTFIIELERSREMEIAVYWRDYRSLCALKFLKLEDFLDNQKHKVQLELEPQGLLLAEVTFFNPVIERVPRLKRQKNVFSKQQGKAFLRARQMNVDIGTFVRLLRNAIPTVNNTGTYSPNAHTLQTGEISVEKLSLDFDSPMKVDIRREVTDISTPERQPSTEPLSAPDVTTPIRTHSLSSKQKKTSLSLQDFRLIAVLGRGHFGKVLLAEYKKSGSMYAIKALKKGDIVARDEVESLMCEKRIFEAVNGAQHPFLVNLFACFQTPEHVCFVMEYTAGGDLMMHIHADVFTEPRAVFYSACVVLGLQFLHDNKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGFGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSEKDAEDVKKQPFFRNMDWEALLLRKLPPPFIPSIGGKEDVSNFDEEFTTEAPTLTPPREPRVLSRKDQDSFRDFDYVSDLC

Target ORF information:

RefSeq Version	XM_005160050.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein kinase N1a (pkn1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160050.3

ORF Insert Sequence:

ATGCTACTGG ATGTCGGTGT CATCGCCGGA GAGAGGAGAA TGTGTTGGTC TGGTGAATGG 
GATCATTGGG AATTTCTGGA TATCCAGAGT GATCCCGGTG GGTTGTCTGG ATTAGAGCGG 
CTTGGCTTGG ACCACAGCTC TGATTACTCT GACTCGAGCG TACAACAGCG ACTGGATAAA 
GAGCGCGAGC GAATCCGTCG GGAAATCCGC AAGGAGCTGA AGATTAAAGA GGGGGCAGAG 
AATCTCCGCA GAGCCACTAC AGATAAACGA AATGCTCAGC AGGTGGACAG CCAGTTGCGC 
TGCTCAAATC GGCGTCTGGA CTCACTCCAT GCTGAATTAC AAGAACTGGA CGCTCATATT 
GTGGTCAAAG GGACCGATGA TACACACGAT GACGCTCCTC AGTCTCCGGG AGCGGAGAGC 
AGGTCATCGG CACACAAGGA GAGAATTGCT GCTTTGGAAA GGCAGTTAAA CATCGAGCTC 
AAAGTCAAAC AGGGGGCAGA AAACATGATC CCCATTTATA CCAACGGTTC CACCAAGGAC 
AAGAAGATGT TGCAAACAGC TCAGCAGATG CTACAGGACA GCAAGACAAA GATTGACATC 
ATCCGCATGC AGATCCGGAA AGCAGTGCAG GCCTACGAAC ACAACGATGA TACACAGGGA 
AAGCCTGATC CATGTGGTAT GGAGCTACGC ATTGAAGAGC TCAGACACCA TTACCGAGTA 
GAGCACGCCG TCGCTGAGGG AGCCAAAAAC GTCCTTAGAC TTCTTGGAGC CAGTAAAGTA 
CAAGACAAGA AGGCCATGTC AGAGGCTCAG TGTCGTCTGA GTGAATCGTC GCAAAGGTTG 
GACCTGCTGC GAGATTCTTT AGATCGACGG TTAGCAGAGC TTCCTGAGGA TCATCCCAAA 
GCTTCTGTTA TCAAAGAGGA GCTGGTTTTA GCATCCTCAC CAGCCTTCAG CTCTCAACAC 
AGCGCCCCCT ACCTGCACAA CCAGTACAGC ACTCTCTCCA AACCCTCACC CCTCACTGGT 
CGGCTCCAGG TGACATTATT GGGCTGTATG GGTCTTCTGG AGGTGGTTCC CGGACGCTCT 
CGTGGTAGCC AGGTGGTTTT ACCGTGTTAC AGTCCAGGAG AGGGACGATC ATTCATGCGC 
AGTGGAAAAG GCCTGTATAG TCGCAGTGGC AGCGTTAGTG GAAAAACACC CCTCAAAACC 
GACGAACTCT CCTCGGATGT GAGCGCAGTA TTGAAGCTGG ACAACACTGT GGTGTGTCAG 
ACGGCATGGA AGATGGTTGG AGAACAGGGC TGGGATCAGA CCTTCATCAT CGAGCTGGAG 
AGATCCAGAG AGATGGAGAT TGCCGTGTAC TGGAGAGACT ACCGCTCTCT GTGTGCGCTG 
AAATTTCTCA AGCTGGAGGA TTTCTTGGAC AACCAGAAAC ACAAAGTACA ACTAGAGCTA 
GAACCACAGG GACTGCTGCT GGCAGAGGTT ACTTTCTTTA ACCCTGTGAT TGAGAGGGTT 
CCACGGCTAA AACGGCAGAA GAATGTTTTC TCCAAGCAGC AGGGTAAAGC ATTTTTGCGA 
GCTCGACAGA TGAACGTTGA TATTGGCACA TTCGTGAGGT TACTGAGGAA CGCCATACCA 
ACCGTCAACA ACACAGGAAC CTACAGCCCT AACGCGCACA CTCTACAGAC TGGGGAGATA 
TCAGTGGAGA AGTTGAGTTT AGATTTTGAT TCACCAATGA AGGTGGACAT CCGGCGGGAG 
GTGACAGATA TAAGCACTCC GGAGAGGCAG CCGTCCACCG AGCCTCTCTC TGCTCCAGAC 
GTCACCACGC CTATCAGAAC ACACTCATTG AGCAGTAAAC AGAAGAAGAC TTCTCTGAGC 
CTGCAGGATT TCCGACTAAT TGCAGTTCTT GGCAGAGGCC ATTTTGGAAA GGTGCTGCTG 
GCGGAATATA AGAAGTCAGG GAGTATGTAT GCCATTAAAG CTCTAAAGAA AGGAGACATT 
GTAGCCAGAG ATGAAGTAGA AAGTTTGATG TGTGAGAAGC GTATTTTCGA GGCAGTGAAC 
GGTGCACAAC ACCCCTTCCT TGTCAACCTG TTTGCGTGTT TCCAGACTCC AGAACATGTG 
TGTTTTGTGA TGGAGTACAC AGCAGGCGGT GACCTCATGA TGCACATACA TGCAGACGTC 
TTCACCGAGC CACGGGCTGT GTTTTATTCT GCTTGTGTAG TTTTAGGGCT TCAGTTTTTA 
CATGACAACA AGATTGTGTA CCGAGATCTT AAATTAGACA ACTTGCTGCT TGACACAGAG 
GGTTATGTGA AGATTGCCGA CTTTGGATTG TGTAAAGAAG GTATGGGTTT TGGGGACCGG 
ACCAGTACGT TCTGCGGGAC TCCAGAGTTT CTGGCTCCAG AGGTTTTGAC AGACACATCG 
TACACCCGTG CTGTGGACTG GTGGGGTCTC GGAGTGCTTA TATATGAAAT GTTGGTGGGA 
GAGTCCCCAT TCCCAGGTGA TGACGAAGAG GAAGTTTTTG ACAGCATAGT GAATGATGAA 
GTTCGATACC CCAGGTTCCT GTCCGCAGAG GCCATTGGCA TCATGAGGAG GTTACTAAGG 
AGAAATCCAG AAAGACGACT GGGCTCCAGT GAGAAAGATG CAGAAGATGT AAAGAAACAG 
CCTTTCTTCA GGAATATGGA CTGGGAGGCT CTTCTCCTCC GCAAACTCCC TCCTCCTTTT 
ATTCCATCCA TCGGAGGAAA AGAAGACGTC AGCAACTTTG ATGAGGAGTT CACGACAGAA 
GCGCCGACTC TCACTCCTCC CCGCGAGCCT CGAGTGCTTT CGCGCAAAGA TCAGGACAGT 
TTCCGGGACT TCGACTATGT GTCTGACCTC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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pkn1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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