Gfm1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gfm1
Entrez Gene ID	28030
Full Name	G elongation factor, mitochondrial 1
Synonyms	AW545374,D3Wsu133e,Gfm
General protein information	Preferred Names G elongation factor, mitochondrial 1 Names elongation factor G, mitochondrial EF-Gmt G elongation factor 1 elongation factor G 1, mitochondrial elongation factor G1 mEF-G 1 mitochondrial
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 E1\|3 30.96 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_138591.2	NP_613057.2	elongation factor G, mitochondrial

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	SPBC1306.01c	NP_595472.2
Homo sapiens (human)	GFM1	NP_079272.4
Bos taurus (cattle)	GFM1	NP_001193078.1
Gallus gallus (chicken)	GFM1	XP_001233639.1
Saccharomyces cerevisiae (baker's yeast)	MEF1	NP_013170.1
Arabidopsis thaliana (thale cress)	AT2G45030	NP_182029.1
Magnaporthe oryzae (rice blast fungus)	MGG_14797	XP_003714541.1
Canis lupus familiaris (dog)	GFM1	XP_534320.2
Arabidopsis thaliana (thale cress)	AT1G45332	NP_175135.1
Kluyveromyces lactis	KLLA0D05467g	XP_453304.1
Mus musculus (house mouse)	Gfm1	NP_613057.2
Rattus norvegicus (Norway rat)	Gfm1	NP_446077.1
Caenorhabditis elegans (roundworm)	CELE_F29C12.4	NP_496787.1
Anopheles gambiae (African malaria mosquito)	EFGM_ANOGA	XP_318822.4
Eremothecium gossypii	AGOS_ABR227C	NP_983176.2
Pan troglodytes (chimpanzee)	GFM1	XP_001154618.1
Macaca mulatta (Rhesus monkey)	GFM1	XP_001094832.2
Danio rerio (zebrafish)	gfm1	NP_001073463.1
Drosophila melanogaster (fruit fly)	ico	NP_609105.1
Oryza sativa (rice)	Os03g0565500	NP_001050501.1
Neurospora crassa	NCU02955	XP_965342.2
Xenopus tropicalis (tropical clawed frog)	gfm1	XP_002933285.1

Gene Ontology
Bibliography

Related articles in PubMed

A global genomic transcriptional code associated with CNS-expressed genes.
Bailey PJ, Klos JM, Andersson E, Karlén M, Källström M, Ponjavic J, Muhr J, Lenhard B, Sandelin A, Ericson J
Experimental cell research312(16)3108-19(2006 Oct)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

Cloning and characterization of human and mouse mitochondrial elongation factor G, GFM and Gfm, and mapping of GFM to human chromosome 3q25.1-q26.2.
Gao J, Yu L, Zhang P, Jiang J, Chen J, Peng J, Wei Y, Zhao S
Genomics74(1)109-14(2001 May)

Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome.
Ko MS, Threat TA, Wang X, Horton JH, Cui Y, Wang X, Pryor E, Paris J, Wells-Smith J, Kitchen JR, Rowe LB, Eppig J, Satoh T, Brant L, Fujiwara H, Yotsumoto S, Nakashima H
Human molecular genetics7(12)1967-78(1998 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gfm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gfm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13116	NM_138591.2 Latest version!	Mus musculus G elongation factor, mitochondrial 1 (Gfm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gfm1 ( NM_138591.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_613057.2 Mus musculus G elongation factor, mitochondrial 1 (Gfm1), mRNA.

Price & Availability↑

CloneID	OMu13116
Clone ID Related Accession (Same CDS sequence)	NM_138591.2
Accession Version	NM_138591.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Mus musculus(house mouse)
Product	elongation factor G, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124190.4. On Mar 21, 2008 this sequence version replaced NM_138591.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC031772.1, AF315511.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGCGGCTGC TGCGGGTCGC CGCAGCCCTG GGGCGTGGAC CGTTCCCTCG GGTCCCTGCA 
GTCCTGGGCT GGCAGGGGAA GCAGGCTGAT TGGAAGACCC GCCGATGGTG TTCGTCAGGG 
CCAGTTCCCA ATGAAAAAAT ACGGAACATT GGGATCTCAG CTCACATTGA TTCTGGGAAG 
ACGACGCTAA CAGAACGGGT GCTATACTAC ACTGGCAGGA TTGCCACGAT GCACGAGGTG 
AAAGGTAAAG ATGGAGTTGG CGCTGTCATG GATTCCATGG AGCTGGAGAG GCAGCGAGGG 
ATCACGATCC AGTCAGCAGC TACGTATACC ATGTGGAAAG ACATCAATAT CAACATCATA 
GACACGCCAG GCCACGTGGA CTTTACCATA GAAGTGGAAA GAGCCCTGCG GGTCCTGGAC 
GGTGCAGTCC TTGTTCTCTG TGCGGTTGGC GGAGTCCAGT GCCAGACCAT GACAGTCAGT 
CGTCAGATGA AGCGCTACAA CGTTCCCTTT TTAACCTTCA TTAACAAACT GGACCGAATG 
GGCTCTAACC CATCCAGGGC CCTCCAACAA ATGAGATCTA AACTAAATCA CAATGCTGCA 
TTTGTACAAA TACCCATTGG TTTAGAGGGT GATTTTAAGG GAATTATAGA CCTCATTGAA 
GAACGAGCTA TTTACTTTGA TGGAGACTTT GGTCAGATTG TCCGATATGA CGAGATTCCG 
GCTGGATTGC GGGCCGCAGC CGCTGACCAC CGGCAGGAGC TCATTGAGTG CGTTGCTAAC 
TCAGATGAGC AGCTTGGTGA GCTCTTCCTG GAAGAAAAGA TCCCCTCAGT TTCCGACTTA 
AAGCGAGCAA TCCGGAGAGC TACTCTGAGT CGATCCTTCA CTCCTGTATT TTTGGGAAGT 
GCTTTGAAAA ACAAAGGAGT TCAACCTCTT TTGGACGCTG TTTTAGAATA CCTGCCCAAT 
CCATCTGAGG TCCAGAATTA CGCTATACTC AATCAGAATG ACTCAAAAGA GAAGACCAAA 
ATCTTAATGA ACCCCCAAAG AGATGATTCG CACCCATTTG TAGGCCTGGC TTTTAAACTG 
GAGGCGGGCC GTTTTGGACA GCTGACTTAT GTCCGAAATT ATCAAGGTGA ACTGAAGAAG 
GGAAGCACCA TCTACAACAC GAGGACCGGG AAGAAAGTGC GCGTGCAGCG GCTAGTGCGC 
ATGCACGCCG ACATGATGGA GGATGTTGAA GAAGTATATG CTGGAGACAT CTGTGCATTG 
TTTGGCATTG ACTGTGCAAG TGGAGATACA TTCACAAACA AAGATAACAG TGACCTTTCT 
ATGGAATCGA TTCATGTTCC TGAGCCTGTC ATTTCAATAG CAATGAGGCC TTCTAACAAG 
AATGATCTGG AGAAATTTTC CAAAGGTATT GGCAGGTTTA CACGGGAAGA CCCCACATTT 
AAAGTCCACT TTGACCCTGA GAGCAAGGAG ACCATTGTGT CAGGGATGGG AGAGCTGCAC 
CTGGAAATCT ATGCTCAGAG AATGGAGAGA GAATATGGCT GCCCTTGTAT CACAGGAAAA 
CCCAAAGTAG CCTTTAGAGA GACCATTGTT GCCCCAGTGC CGTTTGATTT TACACATAAA 
AAGCAGTCGG GTGGTGCCGG CCAGTTTGGA AAAGTAATAG GAGTGCTGGA GCCCTTGCCC 
CCAGAGGACT ACACGAAGTT GGAGTTTTCT GATGAGACAT TTGGGTCCAA TGTTCCAAAG 
CAGTTTGTCC CCGCTGTTGA AAAGGGGTTT CTGGATGCCT GCGAAAAAGG CCCTCTTTCT 
GGTCACAAGC TTTCTGGGCT ACGGTTTGTT CTGCAAGATG GAGCACACCA CATGGTTGAT 
TCGAATGAAA TCTCTTTCAT CCGAGCTGGA GAAGGTGCTC TTAAACAAGC CTTGGCAAAT 
GGCACACTAT GTATTATTGA GCCTATTATG TCTGTGGAAG TTATAGCCCC CAATGAATTC 
CAGGGAACAG TGTTCGCAGG GATTAACCGG CGGCATGGAG TGATTACAGG GCAGGACGGC 
ATTGAGGACT ACTTCACACT GTATGCAGAT GTTCCCCTAA ATAATATGTT TGGTTATTCC 
ACTGAGCTTA GGTCTTGCAC AGAGGGAAAA GGAGAGTACA CGATGGAGTA CTGCAGGTAC 
CAGCCGTGTT CACCATCCAC ACAGGAAGAG CTCATCAATA AGTATTTGGA AGCTACAGGT 
CAGCTTCCCG TAAAAAAAGG GAAAGCCAAG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_613057.2
CDS	125..2380
Translation	MRLLRVAAALGRGPFPRVPAVLGWQGKQADWKTRRWCSSGPVPNEKIRNIGISAHIDSGKTTLTERVLYYTGRIATMHEVKGKDGVGAVMDSMELERQRGITIQSAATYTMWKDININIIDTPGHVDFTIEVERALRVLDGAVLVLCAVGGVQCQTMTVSRQMKRYNVPFLTFINKLDRMGSNPSRALQQMRSKLNHNAAFVQIPIGLEGDFKGIIDLIEERAIYFDGDFGQIVRYDEIPAGLRAAAADHRQELIECVANSDEQLGELFLEEKIPSVSDLKRAIRRATLSRSFTPVFLGSALKNKGVQPLLDAVLEYLPNPSEVQNYAILNQNDSKEKTKILMNPQRDDSHPFVGLAFKLEAGRFGQLTYVRNYQGELKKGSTIYNTRTGKKVRVQRLVRMHADMMEDVEEVYAGDICALFGIDCASGDTFTNKDNSDLSMESIHVPEPVISIAMRPSNKNDLEKFSKGIGRFTREDPTFKVHFDPESKETIVSGMGELHLEIYAQRMEREYGCPCITGKPKVAFRETIVAPVPFDFTHKKQSGGAGQFGKVIGVLEPLPPEDYTKLEFSDETFGSNVPKQFVPAVEKGFLDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEISFIRAGEGALKQALANGTLCIIEPIMSVEVIAPNEFQGTVFAGINRRHGVITGQDGIEDYFTLYADVPLNNMFGYSTELRSCTEGKGEYTMEYCRYQPCSPSTQEELINKYLEATGQLPVKKGKAKN

Target ORF information:

RefSeq Version	NM_138591.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus G elongation factor, mitochondrial 1 (Gfm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138591.2

ORF Insert Sequence:

ATGCGGCTGC TGCGGGTCGC CGCAGCCCTG GGGCGTGGAC CGTTCCCTCG GGTCCCTGCA 
GTCCTGGGCT GGCAGGGGAA GCAGGCTGAT TGGAAGACCC GCCGATGGTG TTCGTCAGGG 
CCAGTTCCCA ATGAAAAAAT ACGGAACATT GGGATCTCAG CTCACATTGA TTCTGGGAAG 
ACGACGCTAA CAGAACGGGT GCTATACTAC ACTGGCAGGA TTGCCACGAT GCACGAGGTG 
AAAGGTAAAG ATGGAGTTGG CGCTGTCATG GATTCCATGG AGCTGGAGAG GCAGCGAGGG 
ATCACGATCC AGTCAGCAGC TACGTATACC ATGTGGAAAG ACATCAATAT CAACATCATA 
GACACGCCAG GCCACGTGGA CTTTACCATA GAAGTGGAAA GAGCCCTGCG GGTCCTGGAC 
GGTGCAGTCC TTGTTCTCTG TGCGGTTGGC GGAGTCCAGT GCCAGACCAT GACAGTCAGT 
CGTCAGATGA AGCGCTACAA CGTTCCCTTT TTAACCTTCA TTAACAAACT GGACCGAATG 
GGCTCTAACC CATCCAGGGC CCTCCAACAA ATGAGATCTA AACTAAATCA CAATGCTGCA 
TTTGTACAAA TACCCATTGG TTTAGAGGGT GATTTTAAGG GAATTATAGA CCTCATTGAA 
GAACGAGCTA TTTACTTTGA TGGAGACTTT GGTCAGATTG TCCGATATGA CGAGATTCCG 
GCTGGATTGC GGGCCGCAGC CGCTGACCAC CGGCAGGAGC TCATTGAGTG CGTTGCTAAC 
TCAGATGAGC AGCTTGGTGA GCTCTTCCTG GAAGAAAAGA TCCCCTCAGT TTCCGACTTA 
AAGCGAGCAA TCCGGAGAGC TACTCTGAGT CGATCCTTCA CTCCTGTATT TTTGGGAAGT 
GCTTTGAAAA ACAAAGGAGT TCAACCTCTT TTGGACGCTG TTTTAGAATA CCTGCCCAAT 
CCATCTGAGG TCCAGAATTA CGCTATACTC AATCAGAATG ACTCAAAAGA GAAGACCAAA 
ATCTTAATGA ACCCCCAAAG AGATGATTCG CACCCATTTG TAGGCCTGGC TTTTAAACTG 
GAGGCGGGCC GTTTTGGACA GCTGACTTAT GTCCGAAATT ATCAAGGTGA ACTGAAGAAG 
GGAAGCACCA TCTACAACAC GAGGACCGGG AAGAAAGTGC GCGTGCAGCG GCTAGTGCGC 
ATGCACGCCG ACATGATGGA GGATGTTGAA GAAGTATATG CTGGAGACAT CTGTGCATTG 
TTTGGCATTG ACTGTGCAAG TGGAGATACA TTCACAAACA AAGATAACAG TGACCTTTCT 
ATGGAATCGA TTCATGTTCC TGAGCCTGTC ATTTCAATAG CAATGAGGCC TTCTAACAAG 
AATGATCTGG AGAAATTTTC CAAAGGTATT GGCAGGTTTA CACGGGAAGA CCCCACATTT 
AAAGTCCACT TTGACCCTGA GAGCAAGGAG ACCATTGTGT CAGGGATGGG AGAGCTGCAC 
CTGGAAATCT ATGCTCAGAG AATGGAGAGA GAATATGGCT GCCCTTGTAT CACAGGAAAA 
CCCAAAGTAG CCTTTAGAGA GACCATTGTT GCCCCAGTGC CGTTTGATTT TACACATAAA 
AAGCAGTCGG GTGGTGCCGG CCAGTTTGGA AAAGTAATAG GAGTGCTGGA GCCCTTGCCC 
CCAGAGGACT ACACGAAGTT GGAGTTTTCT GATGAGACAT TTGGGTCCAA TGTTCCAAAG 
CAGTTTGTCC CCGCTGTTGA AAAGGGGTTT CTGGATGCCT GCGAAAAAGG CCCTCTTTCT 
GGTCACAAGC TTTCTGGGCT ACGGTTTGTT CTGCAAGATG GAGCACACCA CATGGTTGAT 
TCGAATGAAA TCTCTTTCAT CCGAGCTGGA GAAGGTGCTC TTAAACAAGC CTTGGCAAAT 
GGCACACTAT GTATTATTGA GCCTATTATG TCTGTGGAAG TTATAGCCCC CAATGAATTC 
CAGGGAACAG TGTTCGCAGG GATTAACCGG CGGCATGGAG TGATTACAGG GCAGGACGGC 
ATTGAGGACT ACTTCACACT GTATGCAGAT GTTCCCCTAA ATAATATGTT TGGTTATTCC 
ACTGAGCTTA GGTCTTGCAC AGAGGGAAAA GGAGAGTACA CGATGGAGTA CTGCAGGTAC 
CAGCCGTGTT CACCATCCAC ACAGGAAGAG CTCATCAATA AGTATTTGGA AGCTACAGGT 
CAGCTTCCCG TAAAAAAAGG GAAAGCCAAG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A global genomic transcriptional code associated with CNS-expressed genes.
Experimental cell research312(16)3108-19(2006 Oct)
Bailey PJ,Klos JM,Andersson E,Karlén M,Källström M,Ponjavic J,Muhr J,Lenhard B,Sandelin A,Ericson J

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

Cloning and characterization of human and mouse mitochondrial elongation factor G, GFM and Gfm, and mapping of GFM to human chromosome 3q25.1-q26.2.
Genomics74(1)109-14(2001 May)
Gao J,Yu L,Zhang P,Jiang J,Chen J,Peng J,Wei Y,Zhao S

Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome.
Human molecular genetics7(12)1967-78(1998 Nov)
Ko MS,Threat TA,Wang X,Horton JH,Cui Y,Wang X,Pryor E,Paris J,Wells-Smith J,Kitchen JR,Rowe LB,Eppig J,Satoh T,Brant L,Fujiwara H,Yotsumoto S,Nakashima H

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Gfm1 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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