Skint5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Skint5
Entrez Gene ID	242627
Full Name	selection and upkeep of intraepithelial T cells 5
Synonyms	Skint-5
General protein information	Preferred Names selection and upkeep of intraepithelial T cells 5 Names selection and upkeep of intraepithelial T-cells protein 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D1

mRNA and Protein(s)

mRNA	Protein	Name
XM_030253570.1	XP_030109430.1	selection and upkeep of intraepithelial T-cells protein 5 isoform X2
XM_030253569.1	XP_030109429.1	selection and upkeep of intraepithelial T-cells protein 5 isoform X1
NM_001167876.1	NP_001161348.1	selection and upkeep of intraepithelial T-cells protein 5 isoform 1 precursor
NM_001167878.1	NP_001161350.1	selection and upkeep of intraepithelial T-cells protein 5 isoform 2 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Skint6	NP_001096669.1
Mus musculus (house mouse)	Skint5	NP_001161348.1

Gene Ontology
Bibliography

Related articles in PubMed

Skint1, the prototype of a newly identified immunoglobulin superfamily gene cluster, positively selects epidermal gammadelta T cells.
Boyden LM, Lewis JM, Barbee SD, Bas A, Girardi M, Hayday AC, Tigelaar RE, Lifton RP
Nature genetics40(5)656-62(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Skint5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Skint5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13382	NM_001167878.1 Latest version!	Mus musculus selection and upkeep of intraepithelial T cells 5 (Skint5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu13392	NM_001167876.1 Latest version!	Mus musculus selection and upkeep of intraepithelial T cells 5 (Skint5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74112	XM_030253569.1 Latest version!	Mus musculus selection and upkeep of intraepithelial T cells 5 (Skint5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74113	XM_030253570.1 Latest version!	Mus musculus selection and upkeep of intraepithelial T cells 5 (Skint5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Skint5 ( NM_001167878.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001161350.1 Mus musculus selection and upkeep of intraepithelial T cells 5 (Skint5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13382
Clone ID Related Accession (Same CDS sequence)	NM_001167878.1
Accession Version	NM_001167878.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550678400000
Organism	Mus musculus(house mouse)
Product	selection and upkeep of intraepithelial T-cells protein 5 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EU099302.1. On Nov 20, 2009 this sequence version replaced XM_144002.6. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: EU099302.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849378, SAMN00849379 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END##

ATGGGAGCTG TAGGAATACC TCTTACCGCA CACTGCGTTG TTTTGTTTCT CCTCCAGATG 
GTAGCACTGA GTTCAGAACA ATTCACTGTG AATGGCTTAG AGAGTCCAGT CTTGGTTCCA 
TTGGATGGAA ACCTAGAACT CAGTTGTCAG TTGTCTCCAC CACAGCAAGC AAAACACATG 
GAAATTCGCT GGTTCAAGAA TCGCTATTCA GAGCCAGTAT ACCTGTATAG AAATGGTAAA 
GACCTTTTTG GAGAAATTGT CTACAAGTAT GTAGAGAGAA CTGAACTCCT GAAAGATGAC 
ATCGGGAAAG GGAAAGTAAC CCTCAGGATC TTTAAAGTAA CTAGTGTTGA CAGTGGGTCC 
TACCACTGCT TCTTCAAAGA TGGCAAGTTC TATGAAGAGC ATATCATAGA CGTCAAGGTC 
ACAGCTACAA GCTCAGACAT ACAGATTCTT ATGCATCCCC CAAATATCAA AGGTGTGAGG 
TTGGAGTGTC ATTCAGGCGG TTGGTTCCCA CAGCCTCACA TGGAATGGAG AGATAGCAAA 
GGAGAGTACA TTCCAGCTAC ATCAAAATCC CATTCACAGG ATGAAAATGA ATTTTTCAAC 
ATGACAATGG ACCTTTTTAT TAAATCCAAC TCTCACTGGA GTGCAACCTG CTATATTCAA 
AACTTTGTAA CTCACCAAGA AGAAAGCATA AGTATTGTCC TACCAGGTGT GTGGGATTCA 
TGGTGTCCTG CTTGGATAAT GATTACTTTG CTGATTGTCA TCCACATGAC TTACTATATT 
AAATTATACC GCACATATGC GACAAAAGAG ATGCTTTTGA AAAGTATTCA ATCAAGTTCT 
CAAAGTTCCA CTGTGGATAC AGAGAAAAAA GAGTGGCTGT TGAAAAGTAT TCAATCAAGG 
ATTCAAAGTT CCAGTGTGGA TGCAGAGAAA AAAGAGTGGC TGTTGAAATT TATTCAATCA 
AGTATTCAAA GTTCCAGTGT GGATCTAGAA AAAAAAGAGT GGCTGTTGAA AAGCATTCAA 
TCAAGTATTC AAAGTGCCAT TGTGGATCGA GGAACAAAAG AGTGGCTGTT GGAAAGCATT 
CAATCAAGTA TTCTAAGTTC CATTGTGGAT CAAGGGACAA AAGTGTTGCT GTTGAAAAGT 
ATTCAATCAA GCATTCAAAG TTCCACTCTG GATATAGAAA CAAAGGAGTT GCTGTTGAAA 
AGCATTCAAT CAAGTATTCA AAGTTCCATT ATGGATCTAG GAACAAAAGA GTTGCTGTTG 
AAAATAATTC AATCGAGTAT TCAAAGTTCC AGTGTGGATC TAGGAACAAA AGAGTTGCTG 
TTGAAAATAA TTCAATCGAG TATTCAAAGT TCCACTGTGG ATCTAGGAAC AAAAGAGTTG 
CTGTTGAAAA TAATTCAATC GAGTATTCAA AGTTCCAGTG TGGATCTAGG AACAAAAGAG 
TTGCTGTTGA AAATAATTCA ATCGAGTATT CAAAGTTCCA CTGTGGATCT AGGAACAAAA 
GAGTTGCTGT TGAAAATAAT TCAATCGAGT ATTCAAAGTT CCAGTGTGGA TCTAGGAGCA 
AAAATGGGGC TGTTGGAAAG CATTTTGTCA AGTATTCAAA GTTCCAATGT GGATCTAGAG 
ACAAAAGTGT TGCTGTTGAA AAGTATTCAA TCAAGTATTC AAAGTTCCAC TGTGTATCTA 
GGAATAAAAC AATGGCTGTT GGAAAGAATT GAATCAATTA TTCAAAGTTC CAGTGTGTAT 
CTAGATACAA AAGAGTTGCT GTTGAAAATT ATTCAATCAA GTATTGAAAG TTACAGTGTG 
GATCTAGGGA ACAAAGAGTT TCTGTTGAAA ATTATTCTAT CAAGTATCCA AAGTTCCAGT 
GTGGATCTAG GGACAAAAGA GTTGCTGGTG AAATTTTTTC AATCAAATAT TCAAAGTTCC 
AGTGTGATTC CAGGGACAAA AAAGTTGCTG GTGAAAATTA TTCAATCAAG TCTTCAAAGT 
TCCAGTGTGG ATCTAGGGAC AAAAAAGGTG TTGTTGGATA TTATTCAAAC AAGTATTCAA 
AACTCCAATG TGCATACAGA GAGAAAAGGG TTGCTGTTGA AAATTATTCA ATCAAGTGTT 
CAAAGTTCCA GTGTGGATCA AGGGACAAAA GAGATGCTGT TGGAAATTAT TCAATCAAGT 
ATTCAAAATT CCAGTGTGAA TCAATGGACA AAAGATTTGC TGCTGAAAAT TATTCAATCA 
ATTATTCAAA GTTCCAGTGT GGATCTAGGG ACAAAAGGGT TCCTTTTGAA AATTATTCAA 
TCAAGTATTC AAAGTTCCAG TGTGGATATA GGGACCAAAT CCATGCTGTT GAAAAAAACA 
GGATTGATTT TGAAGAGTTC CATTGTGAAT CCAGGGACAG AGTTGCTGTT TCAACTAATT 
GAATCAATTC GTCATAGTTC CAGTGTGAAT TCAGAGACAA AAAAGATGCT GTCTGAAATA 
ACTCAATCAA CTGTTCAAAG TTCCAGTGTG AATCCAGTGA CAGAAGAGAT GATAGAGGAG 
AAATATCAAT TGCTTCTTCA AAGTTCCAGT GTGAATCTAG AAGCAGAAAA TATACTGAAT 
GTTACAACCC AATGGATTCT TCAAATTTCC AGTGTGAATC AAGGAAAAGA AATGTTGCTC 
TTGGATAAAA TTCAGCAGAT TCTTCAAATT TCAAGTGTGA ATCCAGAAAC AAAAGATTTG 
TTGTTGGAAA AAATTGAGTT GATTCTTCAA AATCCCGGTG TGCATCAAGA GACAAAAGAT 
TTGCTGTTGG AAAGAATTCA GCTGATCCTT CAAAATTCAA GTGTGCAAAA AGAGACAAAA 
TATTTGCTGT TGGAAAGAAT TCGGTCGATC CTTCAAAGTT CGTGTGTGCA AAAAGAGACA 
AAAGATTTGC TGTTGGAAAG AATTCAGTCA CTCCTTCAAA GTTCGTGTGT TCAAAAAGAG 
ACAAAAGATT TGTTGTTGGA AAAAATTCAG TTGATTCTTC AAAATTCCAC TGTGCATCAA 
GAAACAAAAG ATTTGTTGTT GGAAAGAATT CAGTTGTTTC TTCAAAATTC CACTGTGGAT 
CAAGAGACAA GTTATTTACT CCTGAAAAGA ATTCAGTTGA TTCTTCAAAG TTCCAGTGTG 
AAAAAAGAGA CAAAAGAGTT CGTGTTGTAT ATAATTGAGT CGATTCTTCA CAGTTCCAGT 
GTGCATCAAG AGACGAAAGA TTTGCTGTTC GACAGAGTTC AGTTGATTCT TGAAAGTTCC 
AGTGTGCAGC AAGGGTTGAA ATGTTTGCTG TTGAACATGA TTCAGGTGAT TTTTCAAAGG 
ACAAGTGTGC AAAAAGAGAT GAAAGATTTG CTATTCAACA GAATTCCGTT GATTCTTGAA 
AGTTCCAGTG TGCAGCAAGA GACAAAAGAT AAGCTGTTGG ACATAATTCA GTCGATTCTT 
CAAAGGACCA GTGTACAAGA AGAGACAAAA GATAAGCTGT TGGACATAAT TCAGTCGATT 
CTTCAAAGAA GCAGTGTGCA AAAAGATACG ATAGATACGC TGTTGGACAG AATTCAGTTG 
ATTCTTCAAA GAACCAGTGT GCAAAAAGAG ATGAAAGACT TCCTGCTGGA CAGAATTAAG 
TCAATTCTTG AAAGTTTCAG TGTGCATCAA GAGACGAAAA AATTGCTGTT GAACAGAATT 
ATGTCGATTC TTGAAAGTTC CACTGTGCAT CAAGAGACAA AAAAATTGCT GTTGGACAGA 
ATTCATTCGA TTCTTAAAAG TTCCAGTGTG CAAAAAGAGA CAAAAAACTT GCTGTTGGAC 
AGAATTCATT CGATTCTTAA AAGTTCCAGT GTGCAAAAAG AGTCCTGCAA TAAGAGAAAT 
CCTTTTTGGA AAAAGTATGC ATTGGACCTC GGAATTTCAG TGTTCACAAT TATAGTAGTC 
ACTCTCATTA TGCATTTAAA ACAAAGAGAA GCAGACCAAC ATTTTGAACT GAATACTTTG 
TGGTCAAAAG ATACTAGTGT GATCCTGTGT GTGCTGATCA TGTTTAACAA CAGGCTCAAG 
GCATTGATAT ACTTCAGACT GTATGGATTT TCACCTCCAG GTAAAGCACA TAAGTACATA 
GTAAACTACA TATTAAGATT CTCACATCCC GTCTTTTGCA TTGTCTACTC TGCAACAATC 
CTTTATATGT ATCTGCAAAT ACAGAACAAA GATTCCTTAT TTTCCTTGTA TAATAGCTGG 
ATGGTAGAAA TGGAAATGGT TCTAATATTT CTGCTCGTAA TTTTCAATGT TAAAAACATT 
GCAACTGTAT TGTTATACTT TGACAGTACA ACTCTACGAC TATTCTTCTG GATTAAAGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161350.1
CDS	55..4377
Translation	MGAVGIPLTAHCVVLFLLQMVALSSEQFTVNGLESPVLVPLDGNLELSCQLSPPQQAKHMEIRWFKNRYSEPVYLYRNGKDLFGEIVYKYVERTELLKDDIGKGKVTLRIFKVTSVDSGSYHCFFKDGKFYEEHIIDVKVTATSSDIQILMHPPNIKGVRLECHSGGWFPQPHMEWRDSKGEYIPATSKSHSQDENEFFNMTMDLFIKSNSHWSATCYIQNFVTHQEESISIVLPGVWDSWCPAWIMITLLIVIHMTYYIKLYRTYATKEMLLKSIQSSSQSSTVDTEKKEWLLKSIQSRIQSSSVDAEKKEWLLKFIQSSIQSSSVDLEKKEWLLKSIQSSIQSAIVDRGTKEWLLESIQSSILSSIVDQGTKVLLLKSIQSSIQSSTLDIETKELLLKSIQSSIQSSIMDLGTKELLLKIIQSSIQSSSVDLGTKELLLKIIQSSIQSSTVDLGTKELLLKIIQSSIQSSSVDLGTKELLLKIIQSSIQSSTVDLGTKELLLKIIQSSIQSSSVDLGAKMGLLESILSSIQSSNVDLETKVLLLKSIQSSIQSSTVYLGIKQWLLERIESIIQSSSVYLDTKELLLKIIQSSIESYSVDLGNKEFLLKIILSSIQSSSVDLGTKELLVKFFQSNIQSSSVIPGTKKLLVKIIQSSLQSSSVDLGTKKVLLDIIQTSIQNSNVHTERKGLLLKIIQSSVQSSSVDQGTKEMLLEIIQSSIQNSSVNQWTKDLLLKIIQSIIQSSSVDLGTKGFLLKIIQSSIQSSSVDIGTKSMLLKKTGLILKSSIVNPGTELLFQLIESIRHSSSVNSETKKMLSEITQSTVQSSSVNPVTEEMIEEKYQLLLQSSSVNLEAENILNVTTQWILQISSVNQGKEMLLLDKIQQILQISSVNPETKDLLLEKIELILQNPGVHQETKDLLLERIQLILQNSSVQKETKYLLLERIRSILQSSCVQKETKDLLLERIQSLLQSSCVQKETKDLLLEKIQLILQNSTVHQETKDLLLERIQLFLQNSTVDQETSYLLLKRIQLILQSSSVKKETKEFVLYIIESILHSSSVHQETKDLLFDRVQLILESSSVQQGLKCLLLNMIQVIFQRTSVQKEMKDLLFNRIPLILESSSVQQETKDKLLDIIQSILQRTSVQEETKDKLLDIIQSILQRSSVQKDTIDTLLDRIQLILQRTSVQKEMKDFLLDRIKSILESFSVHQETKKLLLNRIMSILESSTVHQETKKLLLDRIHSILKSSSVQKETKNLLLDRIHSILKSSSVQKESCNKRNPFWKKYALDLGISVFTIIVVTLIMHLKQREADQHFELNTLWSKDTSVILCVLIMFNNRLKALIYFRLYGFSPPGKAHKYIVNYILRFSHPVFCIVYSATILYMYLQIQNKDSLFSLYNSWMVEMEMVLIFLLVIFNVKNIATVLLYFDSTTLRLFFWIKG

Target ORF information:

RefSeq Version	NM_001167878.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus selection and upkeep of intraepithelial T cells 5 (Skint5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167878.1

ORF Insert Sequence:

ATGGGAGCTG TAGGAATACC TCTTACCGCA CACTGCGTTG TTTTGTTTCT CCTCCAGATG 
GTAGCACTGA GTTCAGAACA ATTCACTGTG AATGGCTTAG AGAGTCCAGT CTTGGTTCCA 
TTGGATGGAA ACCTAGAACT CAGTTGTCAG TTGTCTCCAC CACAGCAAGC AAAACACATG 
GAAATTCGCT GGTTCAAGAA TCGCTATTCA GAGCCAGTAT ACCTGTATAG AAATGGTAAA 
GACCTTTTTG GAGAAATTGT CTACAAGTAT GTAGAGAGAA CTGAACTCCT GAAAGATGAC 
ATCGGGAAAG GGAAAGTAAC CCTCAGGATC TTTAAAGTAA CTAGTGTTGA CAGTGGGTCC 
TACCACTGCT TCTTCAAAGA TGGCAAGTTC TATGAAGAGC ATATCATAGA CGTCAAGGTC 
ACAGCTACAA GCTCAGACAT ACAGATTCTT ATGCATCCCC CAAATATCAA AGGTGTGAGG 
TTGGAGTGTC ATTCAGGCGG TTGGTTCCCA CAGCCTCACA TGGAATGGAG AGATAGCAAA 
GGAGAGTACA TTCCAGCTAC ATCAAAATCC CATTCACAGG ATGAAAATGA ATTTTTCAAC 
ATGACAATGG ACCTTTTTAT TAAATCCAAC TCTCACTGGA GTGCAACCTG CTATATTCAA 
AACTTTGTAA CTCACCAAGA AGAAAGCATA AGTATTGTCC TACCAGGTGT GTGGGATTCA 
TGGTGTCCTG CTTGGATAAT GATTACTTTG CTGATTGTCA TCCACATGAC TTACTATATT 
AAATTATACC GCACATATGC GACAAAAGAG ATGCTTTTGA AAAGTATTCA ATCAAGTTCT 
CAAAGTTCCA CTGTGGATAC AGAGAAAAAA GAGTGGCTGT TGAAAAGTAT TCAATCAAGG 
ATTCAAAGTT CCAGTGTGGA TGCAGAGAAA AAAGAGTGGC TGTTGAAATT TATTCAATCA 
AGTATTCAAA GTTCCAGTGT GGATCTAGAA AAAAAAGAGT GGCTGTTGAA AAGCATTCAA 
TCAAGTATTC AAAGTGCCAT TGTGGATCGA GGAACAAAAG AGTGGCTGTT GGAAAGCATT 
CAATCAAGTA TTCTAAGTTC CATTGTGGAT CAAGGGACAA AAGTGTTGCT GTTGAAAAGT 
ATTCAATCAA GCATTCAAAG TTCCACTCTG GATATAGAAA CAAAGGAGTT GCTGTTGAAA 
AGCATTCAAT CAAGTATTCA AAGTTCCATT ATGGATCTAG GAACAAAAGA GTTGCTGTTG 
AAAATAATTC AATCGAGTAT TCAAAGTTCC AGTGTGGATC TAGGAACAAA AGAGTTGCTG 
TTGAAAATAA TTCAATCGAG TATTCAAAGT TCCACTGTGG ATCTAGGAAC AAAAGAGTTG 
CTGTTGAAAA TAATTCAATC GAGTATTCAA AGTTCCAGTG TGGATCTAGG AACAAAAGAG 
TTGCTGTTGA AAATAATTCA ATCGAGTATT CAAAGTTCCA CTGTGGATCT AGGAACAAAA 
GAGTTGCTGT TGAAAATAAT TCAATCGAGT ATTCAAAGTT CCAGTGTGGA TCTAGGAGCA 
AAAATGGGGC TGTTGGAAAG CATTTTGTCA AGTATTCAAA GTTCCAATGT GGATCTAGAG 
ACAAAAGTGT TGCTGTTGAA AAGTATTCAA TCAAGTATTC AAAGTTCCAC TGTGTATCTA 
GGAATAAAAC AATGGCTGTT GGAAAGAATT GAATCAATTA TTCAAAGTTC CAGTGTGTAT 
CTAGATACAA AAGAGTTGCT GTTGAAAATT ATTCAATCAA GTATTGAAAG TTACAGTGTG 
GATCTAGGGA ACAAAGAGTT TCTGTTGAAA ATTATTCTAT CAAGTATCCA AAGTTCCAGT 
GTGGATCTAG GGACAAAAGA GTTGCTGGTG AAATTTTTTC AATCAAATAT TCAAAGTTCC 
AGTGTGATTC CAGGGACAAA AAAGTTGCTG GTGAAAATTA TTCAATCAAG TCTTCAAAGT 
TCCAGTGTGG ATCTAGGGAC AAAAAAGGTG TTGTTGGATA TTATTCAAAC AAGTATTCAA 
AACTCCAATG TGCATACAGA GAGAAAAGGG TTGCTGTTGA AAATTATTCA ATCAAGTGTT 
CAAAGTTCCA GTGTGGATCA AGGGACAAAA GAGATGCTGT TGGAAATTAT TCAATCAAGT 
ATTCAAAATT CCAGTGTGAA TCAATGGACA AAAGATTTGC TGCTGAAAAT TATTCAATCA 
ATTATTCAAA GTTCCAGTGT GGATCTAGGG ACAAAAGGGT TCCTTTTGAA AATTATTCAA 
TCAAGTATTC AAAGTTCCAG TGTGGATATA GGGACCAAAT CCATGCTGTT GAAAAAAACA 
GGATTGATTT TGAAGAGTTC CATTGTGAAT CCAGGGACAG AGTTGCTGTT TCAACTAATT 
GAATCAATTC GTCATAGTTC CAGTGTGAAT TCAGAGACAA AAAAGATGCT GTCTGAAATA 
ACTCAATCAA CTGTTCAAAG TTCCAGTGTG AATCCAGTGA CAGAAGAGAT GATAGAGGAG 
AAATATCAAT TGCTTCTTCA AAGTTCCAGT GTGAATCTAG AAGCAGAAAA TATACTGAAT 
GTTACAACCC AATGGATTCT TCAAATTTCC AGTGTGAATC AAGGAAAAGA AATGTTGCTC 
TTGGATAAAA TTCAGCAGAT TCTTCAAATT TCAAGTGTGA ATCCAGAAAC AAAAGATTTG 
TTGTTGGAAA AAATTGAGTT GATTCTTCAA AATCCCGGTG TGCATCAAGA GACAAAAGAT 
TTGCTGTTGG AAAGAATTCA GCTGATCCTT CAAAATTCAA GTGTGCAAAA AGAGACAAAA 
TATTTGCTGT TGGAAAGAAT TCGGTCGATC CTTCAAAGTT CGTGTGTGCA AAAAGAGACA 
AAAGATTTGC TGTTGGAAAG AATTCAGTCA CTCCTTCAAA GTTCGTGTGT TCAAAAAGAG 
ACAAAAGATT TGTTGTTGGA AAAAATTCAG TTGATTCTTC AAAATTCCAC TGTGCATCAA 
GAAACAAAAG ATTTGTTGTT GGAAAGAATT CAGTTGTTTC TTCAAAATTC CACTGTGGAT 
CAAGAGACAA GTTATTTACT CCTGAAAAGA ATTCAGTTGA TTCTTCAAAG TTCCAGTGTG 
AAAAAAGAGA CAAAAGAGTT CGTGTTGTAT ATAATTGAGT CGATTCTTCA CAGTTCCAGT 
GTGCATCAAG AGACGAAAGA TTTGCTGTTC GACAGAGTTC AGTTGATTCT TGAAAGTTCC 
AGTGTGCAGC AAGGGTTGAA ATGTTTGCTG TTGAACATGA TTCAGGTGAT TTTTCAAAGG 
ACAAGTGTGC AAAAAGAGAT GAAAGATTTG CTATTCAACA GAATTCCGTT GATTCTTGAA 
AGTTCCAGTG TGCAGCAAGA GACAAAAGAT AAGCTGTTGG ACATAATTCA GTCGATTCTT 
CAAAGGACCA GTGTACAAGA AGAGACAAAA GATAAGCTGT TGGACATAAT TCAGTCGATT 
CTTCAAAGAA GCAGTGTGCA AAAAGATACG ATAGATACGC TGTTGGACAG AATTCAGTTG 
ATTCTTCAAA GAACCAGTGT GCAAAAAGAG ATGAAAGACT TCCTGCTGGA CAGAATTAAG 
TCAATTCTTG AAAGTTTCAG TGTGCATCAA GAGACGAAAA AATTGCTGTT GAACAGAATT 
ATGTCGATTC TTGAAAGTTC CACTGTGCAT CAAGAGACAA AAAAATTGCT GTTGGACAGA 
ATTCATTCGA TTCTTAAAAG TTCCAGTGTG CAAAAAGAGA CAAAAAACTT GCTGTTGGAC 
AGAATTCATT CGATTCTTAA AAGTTCCAGT GTGCAAAAAG AGTCCTGCAA TAAGAGAAAT 
CCTTTTTGGA AAAAGTATGC ATTGGACCTC GGAATTTCAG TGTTCACAAT TATAGTAGTC 
ACTCTCATTA TGCATTTAAA ACAAAGAGAA GCAGACCAAC ATTTTGAACT GAATACTTTG 
TGGTCAAAAG ATACTAGTGT GATCCTGTGT GTGCTGATCA TGTTTAACAA CAGGCTCAAG 
GCATTGATAT ACTTCAGACT GTATGGATTT TCACCTCCAG GTAAAGCACA TAAGTACATA 
GTAAACTACA TATTAAGATT CTCACATCCC GTCTTTTGCA TTGTCTACTC TGCAACAATC 
CTTTATATGT ATCTGCAAAT ACAGAACAAA GATTCCTTAT TTTCCTTGTA TAATAGCTGG 
ATGGTAGAAA TGGAAATGGT TCTAATATTT CTGCTCGTAA TTTTCAATGT TAAAAACATT 
GCAACTGTAT TGTTATACTT TGACAGTACA ACTCTACGAC TATTCTTCTG GATTAAAGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skint5 ( NM_001167876.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001161348.1 Mus musculus selection and upkeep of intraepithelial T cells 5 (Skint5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13392
Clone ID Related Accession (Same CDS sequence)	NM_001167876.1
Accession Version	NM_001167876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550678400000
Organism	Mus musculus(house mouse)
Product	selection and upkeep of intraepithelial T-cells protein 5 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EU099301.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: EU099301.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849378, SAMN00849379 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END##

ATGGGAGCTG TAGGAATACC TCTTACCGCA CACTGCGTTG TTTTGTTTCT CCTCCAGATG 
GTAGCACTGA GTTCAGAACA ATTCACTGTG AATGGCTTAG AGAGTCCAGT CTTGGTTCCA 
TTGGATGGAA ACCTAGAACT CAGTTGTCAG TTGTCTCCAC CACAGCAAGC AAAACACATG 
GAAATTCGCT GGTTCAAGAA TCGCTATTCA GAGCCAGTAT ACCTGTATAG AAATGGTAAA 
GACCTTTTTG GAGAAATTGT CTACAAGTAT GTAGAGAGAA CTGAACTCCT GAAAGATGAC 
ATCGGGAAAG GGAAAGTAAC CCTCAGGATC TTTAAAGTAA CTAGTGTTGA CAGTGGGTCC 
TACCACTGCT TCTTCAAAGA TGGCAAGTTC TATGAAGAGC ATATCATAGA CGTCAAGGTC 
ACAGCTACAA GCTCAGACAT ACAGATTCTT ATGCATCCCC CAAATATCAA AGGTGTGAGG 
TTGGAGTGTC ATTCAGGCGG TTGGTTCCCA CAGCCTCACA TGGAATGGAG AGATAGCAAA 
GGAGAGTACA TTCCAGCTAC ATCAAAATCC CATTCACAGG ATGAAAATGA ATTTTTCAAC 
ATGACAATGG ACCTTTTTAT TAAATCCAAC TCTCACTGGA GTGCAACCTG CTATATTCAA 
AACTTTGTAA CTCACCAAGA AGAAAGCATA AGTATTGTCC TACCAGGTGT GTGGGATTCA 
TGGTGTCCTG CTTGGATAAT GATTACTTTG CTGATTGTCA TCCACATGAC TTACTATATT 
AAATTATACC GCACATATGC GACAAAAGAG ATGCTTTTGA AAAGTATTCA ATCAAGTTCT 
CAAAGTTCCA CTGTGGATAC AGAGAAAAAA GAGTGGCTGT TGAAAAGTAT TCAATCAAGG 
ATTCAAAGTT CCAGTGTGGA TGCAGAGAAA AAAGAGTGGC TGTTGAAATT TATTCAATCA 
AGTATTCAAA GTTCCAGTGT GGATCTAGAA AAAAAAGAGT GGCTGTTGAA AAGCATTCAA 
TCAAGTATTC AAAGTGCCAT TGTGGATCGA GGAACAAAAG AGTGGCTGTT GGAAAGCATT 
CAATCAAGTA TTCTAAGTTC CATTGTGGAT CAAGGGACAA AAGTGTTGCT GTTGAAAAGT 
ATTCAATCAA GCATTCAAAG TTCCACTCTG GATATAGAAA CAAAGGAGTT GCTGTTGAAA 
AGCATTCAAT CAAGTATTCA AAGTTCCATT ATGGATCTAG GAACAAAAGA GTTGCTGTTG 
AAAATAATTC AATCGAGTAT TCAAAGTTCC AGTGTGGATC TAGGAACAAA AGAGTTGCTG 
TTGAAAATAA TTCAATCGAG TATTCAAAGT TCCACTGTGG ATCTAGGAAC AAAAGAGTTG 
CTGTTGAAAA TAATTCAATC GAGTATTCAA AGTTCCAGTG TGGATCTAGG AACAAAAGAG 
TTGCTGTTGA AAATAATTCA ATCGAGTATT CAAAGTTCCA CTGTGGATCT AGGAACAAAA 
GAGTTGCTGT TGAAAATAAT TCAATCGAGT ATTCAAAGTT CCAGTGTGGA TCTAGGAGCA 
AAAATGGGGC TGTTGGAAAG CATTTTGTCA AGTATTCAAA GTTCCAATGT GGATCTAGAG 
ACAAAAGTGT TGCTGTTGAA AAGTATTCAA TCAAGTATTC AAAGTTCCAC TGTGTATCTA 
GGAATAAAAC AATGGCTGTT GGAAAGAATT GAATCAATTA TTCAAAGTTC CAGTGTGTAT 
CTAGATACAA AAGAGTTGCT GTTGAAAATT ATTCAATCAA GTATTGAAAG TTACAGTGTG 
GATCTAGGGA ACAAAGAGTT TCTGTTGAAA ATTATTCTAT CAAGTATCCA AAGTTCCAGT 
GTGGATCTAG GGACAAAAGA GTTGCTGGTG AAATTTTTTC AATCAAATAT TCAAAGTTCC 
AGTGTGATTC CAGGGACAAA AAAGTTGCTG GTGAAAATTA TTCAATCAAG TCTTCAAAGT 
TCCAGTGTGG ATCTAGGGAC AAAAAAGGTG TTGTTGGATA TTATTCAAAC AAGTATTCAA 
AACTCCAATG TGCATACAGA GAGAAAAGGG TTGCTGTTGA AAATTATTCA ATCAAGTGTT 
CAAAGTTCCA GTGTGGATCA AGGGACAAAA GAGATGCTGT TGGAAATTAT TCAATCAAGT 
ATTCAAAATT CCAGTGTGAA TCAATGGACA AAAGATTTGC TGCTGAAAAT TATTCAATCA 
ATTATTCAAA GTTCCAGTGT GGATCTAGGG ACAAAAGGGT TCCTTTTGAA AATTATTCAA 
TCAAGTATTC AAAGTTCCAG TGTGGATATA GGGACCAAAT CCATGCTGTT GAAAAAAACA 
GGATTGATTT TGAAGAGTTC CATTGTGAAT CCAGGGACAG AGTTGCTGTT TCAACTAATT 
GAATCAATTC GTCATAGTTC CAGTGTGAAT TCAGAGACAA AAAAGATGCT GTCTGAAATA 
ACTCAATCAA CTGTTCAAAG TTCCAGTGTG AATCCAGTGA CAGAAGAGAT GATAGAGGAG 
AAATATCAAT TGCTTCTTCA AAGTTCCAGT GTGAATCTAG AAGCAGAAAA TATACTGAAT 
GTTACAACCC AATGGATTCT TCAAATTTCC AGTGTGAATC AAGGAAAAGA AATGTTGCTC 
TTGGATAAAA TTCAGCAGAT TCTTCAAATT TCAAGTGTGA ATCCAGAAAC AAAAGATTTG 
TTGTTGGAAA AAATTGAGTT GATTCTTCAA AATCCCGGTG TGCATCAAGA GACAAAAGAT 
TTGCTGTTGG AAAGAATTCA GCTGATCCTT CAAAATTCAA GTGTGCAAAA AGAGACAAAA 
TATTTGCTGT TGGAAAGAAT TCGGTCGATC CTTCAAAGTT CGTGTGTGCA AAAAGAGACA 
AAAGATTTGC TGTTGGAAAG AATTCAGTCA CTCCTTCAAA GTTCGTGTGT TCAAAAAGAG 
ACAAAAGATT TGTTGTTGGA AAAAATTCAG TTGATTCTTC AAAATTCCAC TGTGCATCAA 
GAAACAAAAG ATTTGTTGTT GGAAAGAATT CAGTTGTTTC TTCAAAATTC CACTGTGGAT 
CAAGAGACAA GTTATTTACT CCTGAAAAGA ATTCAGTTGA TTCTTCAAAG TTCCAGTGTG 
AAAAAAGAGA CAAAAGAGTT CGTGTTGTAT ATAATTGAGT CGATTCTTCA CAGTTCCAGT 
GTGCATCAAG AGACGAAAGA TTTGCTGTTC GACAGAGTTC AGTTGATTCT TGAAAGTTCC 
AGTGTGCAGC AAGGGTTGAA ATGTTTGCTG TTGAACATGA TTCAGGTGAT TTTTCAAAGG 
ACAAGTGTGC AAAAAGAGAT GAAAGATTTG CTATTCAACA GAATTCCGTT GATTCTTGAA 
AGTTCCAGTG TGCAGCAAGA GACAAAAGAT AAGCTGTTGG ACATAATTCA GTCGATTCTT 
CAAAGGACCA GTGTACAAGA AGAGACAAAA GATAAGCTGT TGGACATAAT TCAGTCGATT 
CTTCAAAGAA GCAGTGTGCA AAAAGATACG ATAGATACGC TGTTGGACAG AATTCAGTTG 
ATTCTTCAAA GAACCAGTGT GCAAAAAGAG ATGAAAGACT TCCTGCTGGA CAGAATTAAG 
TCAATTCTTG AAAGTTTCAG TGTGCATCAA GAGACGAAAA AATTGCTGTT GAACAGAATT 
ATGTCGATTC TTGAAAGTTC CACTGTGCAT CAAGAAACGA AAAAATTGCT GTTGGAGAGA 
ATTCAGTCGA TTCTTGAAAG TTCCAGTGTG CAGCAAGAGA CAAAAAAATT GCTGTTGGAC 
AGAATTCATT CGATTCTTAA AAGTTCCAGT GTGCAAAAAG AGACAAAAAA CTTGCTGTTG 
GACAGAATTC ATTCGATTCT TAAAAGTTCC AGTGTGCAAA AAGAGTCCTG CAATAAGAGA 
AATCCTTTTT GGAAAAAGTA TGCATTGGAC CTCGGAATTT CAGTGTTCAC AATTATAGTA 
GTCACTCTCA TTATGCATTT AAAACAAAGA GAAGCAGACC AACATTTTGA ACTGAATACT 
TTGTGGTCAA AAGATACTAG TGTGATCCTG TGTGTGCTGA TCATGTTTAA CAACAGGCTC 
AAGGCATTGA TATACTTCAG ACTGTATGGA TTTTCACCTC CAGGTAAAGC ACATAAGTAC 
ATAGTAAACT ACATATTAAG ATTCTCACAT CCCGTCTTTT GCATTGTCTA CTCTGCAACA 
ATCCTTTATA TGTATCTGCA AATACAGAAC AAAGATTCCT TATTTTCCTT GTATAATAGC 
TGGATGGTAG AAATGGAAAT GGTTCTAATA TTTCTGCTCG TAATTTTCAA TGTTAAAAAC 
ATTGCAACTG TATTGTTATA CTTTGACAGT ACAACTCTAC GACTATTCTT CTGGATTAAA 
GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161348.1
CDS	55..4440
Translation	MGAVGIPLTAHCVVLFLLQMVALSSEQFTVNGLESPVLVPLDGNLELSCQLSPPQQAKHMEIRWFKNRYSEPVYLYRNGKDLFGEIVYKYVERTELLKDDIGKGKVTLRIFKVTSVDSGSYHCFFKDGKFYEEHIIDVKVTATSSDIQILMHPPNIKGVRLECHSGGWFPQPHMEWRDSKGEYIPATSKSHSQDENEFFNMTMDLFIKSNSHWSATCYIQNFVTHQEESISIVLPGVWDSWCPAWIMITLLIVIHMTYYIKLYRTYATKEMLLKSIQSSSQSSTVDTEKKEWLLKSIQSRIQSSSVDAEKKEWLLKFIQSSIQSSSVDLEKKEWLLKSIQSSIQSAIVDRGTKEWLLESIQSSILSSIVDQGTKVLLLKSIQSSIQSSTLDIETKELLLKSIQSSIQSSIMDLGTKELLLKIIQSSIQSSSVDLGTKELLLKIIQSSIQSSTVDLGTKELLLKIIQSSIQSSSVDLGTKELLLKIIQSSIQSSTVDLGTKELLLKIIQSSIQSSSVDLGAKMGLLESILSSIQSSNVDLETKVLLLKSIQSSIQSSTVYLGIKQWLLERIESIIQSSSVYLDTKELLLKIIQSSIESYSVDLGNKEFLLKIILSSIQSSSVDLGTKELLVKFFQSNIQSSSVIPGTKKLLVKIIQSSLQSSSVDLGTKKVLLDIIQTSIQNSNVHTERKGLLLKIIQSSVQSSSVDQGTKEMLLEIIQSSIQNSSVNQWTKDLLLKIIQSIIQSSSVDLGTKGFLLKIIQSSIQSSSVDIGTKSMLLKKTGLILKSSIVNPGTELLFQLIESIRHSSSVNSETKKMLSEITQSTVQSSSVNPVTEEMIEEKYQLLLQSSSVNLEAENILNVTTQWILQISSVNQGKEMLLLDKIQQILQISSVNPETKDLLLEKIELILQNPGVHQETKDLLLERIQLILQNSSVQKETKYLLLERIRSILQSSCVQKETKDLLLERIQSLLQSSCVQKETKDLLLEKIQLILQNSTVHQETKDLLLERIQLFLQNSTVDQETSYLLLKRIQLILQSSSVKKETKEFVLYIIESILHSSSVHQETKDLLFDRVQLILESSSVQQGLKCLLLNMIQVIFQRTSVQKEMKDLLFNRIPLILESSSVQQETKDKLLDIIQSILQRTSVQEETKDKLLDIIQSILQRSSVQKDTIDTLLDRIQLILQRTSVQKEMKDFLLDRIKSILESFSVHQETKKLLLNRIMSILESSTVHQETKKLLLERIQSILESSSVQQETKKLLLDRIHSILKSSSVQKETKNLLLDRIHSILKSSSVQKESCNKRNPFWKKYALDLGISVFTIIVVTLIMHLKQREADQHFELNTLWSKDTSVILCVLIMFNNRLKALIYFRLYGFSPPGKAHKYIVNYILRFSHPVFCIVYSATILYMYLQIQNKDSLFSLYNSWMVEMEMVLIFLLVIFNVKNIATVLLYFDSTTLRLFFWIKG

Target ORF information:

RefSeq Version	NM_001167876.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus selection and upkeep of intraepithelial T cells 5 (Skint5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167876.1

ORF Insert Sequence:

ATGGGAGCTG TAGGAATACC TCTTACCGCA CACTGCGTTG TTTTGTTTCT CCTCCAGATG 
GTAGCACTGA GTTCAGAACA ATTCACTGTG AATGGCTTAG AGAGTCCAGT CTTGGTTCCA 
TTGGATGGAA ACCTAGAACT CAGTTGTCAG TTGTCTCCAC CACAGCAAGC AAAACACATG 
GAAATTCGCT GGTTCAAGAA TCGCTATTCA GAGCCAGTAT ACCTGTATAG AAATGGTAAA 
GACCTTTTTG GAGAAATTGT CTACAAGTAT GTAGAGAGAA CTGAACTCCT GAAAGATGAC 
ATCGGGAAAG GGAAAGTAAC CCTCAGGATC TTTAAAGTAA CTAGTGTTGA CAGTGGGTCC 
TACCACTGCT TCTTCAAAGA TGGCAAGTTC TATGAAGAGC ATATCATAGA CGTCAAGGTC 
ACAGCTACAA GCTCAGACAT ACAGATTCTT ATGCATCCCC CAAATATCAA AGGTGTGAGG 
TTGGAGTGTC ATTCAGGCGG TTGGTTCCCA CAGCCTCACA TGGAATGGAG AGATAGCAAA 
GGAGAGTACA TTCCAGCTAC ATCAAAATCC CATTCACAGG ATGAAAATGA ATTTTTCAAC 
ATGACAATGG ACCTTTTTAT TAAATCCAAC TCTCACTGGA GTGCAACCTG CTATATTCAA 
AACTTTGTAA CTCACCAAGA AGAAAGCATA AGTATTGTCC TACCAGGTGT GTGGGATTCA 
TGGTGTCCTG CTTGGATAAT GATTACTTTG CTGATTGTCA TCCACATGAC TTACTATATT 
AAATTATACC GCACATATGC GACAAAAGAG ATGCTTTTGA AAAGTATTCA ATCAAGTTCT 
CAAAGTTCCA CTGTGGATAC AGAGAAAAAA GAGTGGCTGT TGAAAAGTAT TCAATCAAGG 
ATTCAAAGTT CCAGTGTGGA TGCAGAGAAA AAAGAGTGGC TGTTGAAATT TATTCAATCA 
AGTATTCAAA GTTCCAGTGT GGATCTAGAA AAAAAAGAGT GGCTGTTGAA AAGCATTCAA 
TCAAGTATTC AAAGTGCCAT TGTGGATCGA GGAACAAAAG AGTGGCTGTT GGAAAGCATT 
CAATCAAGTA TTCTAAGTTC CATTGTGGAT CAAGGGACAA AAGTGTTGCT GTTGAAAAGT 
ATTCAATCAA GCATTCAAAG TTCCACTCTG GATATAGAAA CAAAGGAGTT GCTGTTGAAA 
AGCATTCAAT CAAGTATTCA AAGTTCCATT ATGGATCTAG GAACAAAAGA GTTGCTGTTG 
AAAATAATTC AATCGAGTAT TCAAAGTTCC AGTGTGGATC TAGGAACAAA AGAGTTGCTG 
TTGAAAATAA TTCAATCGAG TATTCAAAGT TCCACTGTGG ATCTAGGAAC AAAAGAGTTG 
CTGTTGAAAA TAATTCAATC GAGTATTCAA AGTTCCAGTG TGGATCTAGG AACAAAAGAG 
TTGCTGTTGA AAATAATTCA ATCGAGTATT CAAAGTTCCA CTGTGGATCT AGGAACAAAA 
GAGTTGCTGT TGAAAATAAT TCAATCGAGT ATTCAAAGTT CCAGTGTGGA TCTAGGAGCA 
AAAATGGGGC TGTTGGAAAG CATTTTGTCA AGTATTCAAA GTTCCAATGT GGATCTAGAG 
ACAAAAGTGT TGCTGTTGAA AAGTATTCAA TCAAGTATTC AAAGTTCCAC TGTGTATCTA 
GGAATAAAAC AATGGCTGTT GGAAAGAATT GAATCAATTA TTCAAAGTTC CAGTGTGTAT 
CTAGATACAA AAGAGTTGCT GTTGAAAATT ATTCAATCAA GTATTGAAAG TTACAGTGTG 
GATCTAGGGA ACAAAGAGTT TCTGTTGAAA ATTATTCTAT CAAGTATCCA AAGTTCCAGT 
GTGGATCTAG GGACAAAAGA GTTGCTGGTG AAATTTTTTC AATCAAATAT TCAAAGTTCC 
AGTGTGATTC CAGGGACAAA AAAGTTGCTG GTGAAAATTA TTCAATCAAG TCTTCAAAGT 
TCCAGTGTGG ATCTAGGGAC AAAAAAGGTG TTGTTGGATA TTATTCAAAC AAGTATTCAA 
AACTCCAATG TGCATACAGA GAGAAAAGGG TTGCTGTTGA AAATTATTCA ATCAAGTGTT 
CAAAGTTCCA GTGTGGATCA AGGGACAAAA GAGATGCTGT TGGAAATTAT TCAATCAAGT 
ATTCAAAATT CCAGTGTGAA TCAATGGACA AAAGATTTGC TGCTGAAAAT TATTCAATCA 
ATTATTCAAA GTTCCAGTGT GGATCTAGGG ACAAAAGGGT TCCTTTTGAA AATTATTCAA 
TCAAGTATTC AAAGTTCCAG TGTGGATATA GGGACCAAAT CCATGCTGTT GAAAAAAACA 
GGATTGATTT TGAAGAGTTC CATTGTGAAT CCAGGGACAG AGTTGCTGTT TCAACTAATT 
GAATCAATTC GTCATAGTTC CAGTGTGAAT TCAGAGACAA AAAAGATGCT GTCTGAAATA 
ACTCAATCAA CTGTTCAAAG TTCCAGTGTG AATCCAGTGA CAGAAGAGAT GATAGAGGAG 
AAATATCAAT TGCTTCTTCA AAGTTCCAGT GTGAATCTAG AAGCAGAAAA TATACTGAAT 
GTTACAACCC AATGGATTCT TCAAATTTCC AGTGTGAATC AAGGAAAAGA AATGTTGCTC 
TTGGATAAAA TTCAGCAGAT TCTTCAAATT TCAAGTGTGA ATCCAGAAAC AAAAGATTTG 
TTGTTGGAAA AAATTGAGTT GATTCTTCAA AATCCCGGTG TGCATCAAGA GACAAAAGAT 
TTGCTGTTGG AAAGAATTCA GCTGATCCTT CAAAATTCAA GTGTGCAAAA AGAGACAAAA 
TATTTGCTGT TGGAAAGAAT TCGGTCGATC CTTCAAAGTT CGTGTGTGCA AAAAGAGACA 
AAAGATTTGC TGTTGGAAAG AATTCAGTCA CTCCTTCAAA GTTCGTGTGT TCAAAAAGAG 
ACAAAAGATT TGTTGTTGGA AAAAATTCAG TTGATTCTTC AAAATTCCAC TGTGCATCAA 
GAAACAAAAG ATTTGTTGTT GGAAAGAATT CAGTTGTTTC TTCAAAATTC CACTGTGGAT 
CAAGAGACAA GTTATTTACT CCTGAAAAGA ATTCAGTTGA TTCTTCAAAG TTCCAGTGTG 
AAAAAAGAGA CAAAAGAGTT CGTGTTGTAT ATAATTGAGT CGATTCTTCA CAGTTCCAGT 
GTGCATCAAG AGACGAAAGA TTTGCTGTTC GACAGAGTTC AGTTGATTCT TGAAAGTTCC 
AGTGTGCAGC AAGGGTTGAA ATGTTTGCTG TTGAACATGA TTCAGGTGAT TTTTCAAAGG 
ACAAGTGTGC AAAAAGAGAT GAAAGATTTG CTATTCAACA GAATTCCGTT GATTCTTGAA 
AGTTCCAGTG TGCAGCAAGA GACAAAAGAT AAGCTGTTGG ACATAATTCA GTCGATTCTT 
CAAAGGACCA GTGTACAAGA AGAGACAAAA GATAAGCTGT TGGACATAAT TCAGTCGATT 
CTTCAAAGAA GCAGTGTGCA AAAAGATACG ATAGATACGC TGTTGGACAG AATTCAGTTG 
ATTCTTCAAA GAACCAGTGT GCAAAAAGAG ATGAAAGACT TCCTGCTGGA CAGAATTAAG 
TCAATTCTTG AAAGTTTCAG TGTGCATCAA GAGACGAAAA AATTGCTGTT GAACAGAATT 
ATGTCGATTC TTGAAAGTTC CACTGTGCAT CAAGAAACGA AAAAATTGCT GTTGGAGAGA 
ATTCAGTCGA TTCTTGAAAG TTCCAGTGTG CAGCAAGAGA CAAAAAAATT GCTGTTGGAC 
AGAATTCATT CGATTCTTAA AAGTTCCAGT GTGCAAAAAG AGACAAAAAA CTTGCTGTTG 
GACAGAATTC ATTCGATTCT TAAAAGTTCC AGTGTGCAAA AAGAGTCCTG CAATAAGAGA 
AATCCTTTTT GGAAAAAGTA TGCATTGGAC CTCGGAATTT CAGTGTTCAC AATTATAGTA 
GTCACTCTCA TTATGCATTT AAAACAAAGA GAAGCAGACC AACATTTTGA ACTGAATACT 
TTGTGGTCAA AAGATACTAG TGTGATCCTG TGTGTGCTGA TCATGTTTAA CAACAGGCTC 
AAGGCATTGA TATACTTCAG ACTGTATGGA TTTTCACCTC CAGGTAAAGC ACATAAGTAC 
ATAGTAAACT ACATATTAAG ATTCTCACAT CCCGTCTTTT GCATTGTCTA CTCTGCAACA 
ATCCTTTATA TGTATCTGCA AATACAGAAC AAAGATTCCT TATTTTCCTT GTATAATAGC 
TGGATGGTAG AAATGGAAAT GGTTCTAATA TTTCTGCTCG TAATTTTCAA TGTTAAAAAC 
ATTGCAACTG TATTGTTATA CTTTGACAGT ACAACTCTAC GACTATTCTT CTGGATTAAA 
GGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skint5 ( XM_030253569.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109429.1 Mus musculus selection and upkeep of intraepithelial T cells 5 (Skint5), transcript variant X1, mRNA.

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CloneID	OMu74112
Clone ID Related Accession (Same CDS sequence)	XM_030253569.1
Accession Version	XM_030253569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	selection and upkeep of intraepithelial T-cells protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGA GAGCTGACTT AGGATCAACA AACTCAGGTG TGATGGGAGC TGTAGGAATA 
CCTCTTACCG CACACTGCGT TGTTTTGTTT CTCCTCCAGA TGGTAGCACT GAGTTCAGAA 
CAATTCACTG TGAATGGCTT AGAGAGTCCA GTCTTGGTTC CATTGGATGG AAACCTAGAA 
CTCAGTTGTC AGTTGTCTCC ACCACAGCAA GCAAAACACA TGGAAATTCG CTGGTTCAAG 
AATCGCTATT CAGAGCCAGT ATACCTGTAT AGAAATGGTA AAGACCTTTT TGGAGAAATT 
GTCTACAAGT ATGTAGAGAG AACTGAACTC CTGAAAGATG ACATCGGGAA AGGGAAAGTA 
ACCCTCAGGA TCTTTAAAGT AACTAGTGTT GACAGTGGGT CCTACCACTG CTTCTTCAAA 
GATGGCAAGT TCTATGAAGA GCATATCATA GACGTCAAGG TCACAGCTAC AAGCTCAGAC 
ATACAGATTC TTATGCATCC CCCAAATATC AAAGGTGTGA GGTTGGAGTG TCATTCAGGC 
GGTTGGTTCC CACAGCCTCA CATGGAATGG AGAGATAGCA AAGGAGAGTA CATTCCAGCT 
ACATCAAAAT CCCATTCACA GGATGAAAAT GAATTTTTCA ACATGACAAT GGACCTTTTT 
ATTAAATCCA ACTCTCACTG GAGTGCAACC TGCTATATTC AAAACTTTGT AACTCACCAA 
GAAGAAAGCA TAAGTATTGT CCTACCAGGT GTGTGGGATT CATGGTGTCC TGCTTGGATA 
ATGATTACTT TGCTGATTGT CATCCACATG ACTTACTATA TTAAATTATA CCGCACATAT 
GCGACAAAAG AGATGCTTTT GAAAAGTATT CAATCAAGTT CTCAAAGTTC CACTGTGGAT 
ACAGAGAAAA AAGAGTGGCT GTTGAAAAGT ATTCAATCAA GGATTCAAAG TTCCAGTGTG 
GATGCAGAGA AAAAAGAGTG GCTGTTGAAA TTTATTCAAT CAAGTATTCA AAGTTCCAGT 
GTGGATCTAG AAAAAAAAGA GTGGCTGTTG AAAAGCATTC AATCAAGTAT TCAAAGTGCC 
ATTGTGGATC GAGGAACAAA AGAGTGGCTG TTGGAAAGCA TTCAATCAAG TATTCTAAGT 
TCCATTGTGG ATCAAGGGAC AAAAGTGTTG CTGTTGAAAA GTATTCAATC AAGCATTCAA 
AGTTCCACTC TGGATATAGA AACAAAGGAG TTGCTGTTGA AAAGCATTCA ATCAAGTATT 
CAAAGTTCCA TTATGGATCT AGGAACAAAA GAGTTGCTGT TGAAAATAAT TCAATCGAGT 
ATTCAAAGTT CCAGTGTGGA TCTAGGAACA AAAGAGTTGC TGTTGAAAAT AATTCAATCG 
AGTATTCAAA GTTCCACTGT GGATCTAGGA ACAAAAGAGT TGCTGTTGAA AATAATTCAA 
TCGAGTATTC AAAGTTCCAG TGTGGATCTA GGAACAAAAG AGTTGCTGTT GAAAATAATT 
CAATCGAGTA TTCAAAGTTC CACTGTGGAT CTAGGAACAA AAGAGTTGCT GTTGAAAATA 
ATTCAATCGA GTATTCAAAG TTCCAGTGTG GATCTAGGAG CAAAAATGGG GCTGTTGGAA 
AGCATTTTGT CAAGTATTCA AAGTTCCAAT GTGGATCTAG AGACAAAAGT GTTGCTGTTG 
AAAAGTATTC AATCAAGTAT TCAAAGTTCC ACTGTGTATC TAGGAATAAA ACAATGGCTG 
TTGGAAAGAA TTGAATCAAT TATTCAAAGT TCCAGTGTGT ATCTAGATAC AAAAGAGTTG 
CTGTTGAAAA TTATTCAATC AAGTATTGAA AGTTACAGTG TGGATCTAGG GAACAAAGAG 
TTTCTGTTGA AAATTATTCT ATCAAGTATC CAAAGTTCCA GTGTGGATCT AGGGACAAAA 
GAGTTGCTGG TGAAATTTTT TCAATCAAAT ATTCAAAGTT CCAGTGTGAT TCCAGGGACA 
AAAAAGTTGC TGGTGAAAAT TATTCAATCA AGTCTTCAAA GTTCCAGTGT GGATCTAGGG 
ACAAAAAAGG TGTTGTTGGA TATTATTCAA ACAAGTATTC AAAACTCCAA TGTGCATACA 
GAGAGAAAAG GGTTGCTGTT GAAAATTATT CAATCAAGTG TTCAAAGTTC CAGTGTGGAT 
CAAGGGACAA AAGAGATGCT GTTGGAAATT ATTCAATCAA GTATTCAAAA TTCCAGTGTG 
AATCAATGGA CAAAAGATTT GCTGCTGAAA ATTATTCAAT CAATTATTCA AAGTTCCAGT 
GTGGATCTAG GGACAAAAGG GTTCCTTTTG AAAATTATTC AATCAAGTAT TCAAAGTTCC 
AGTGTGGATA TAGGGACCAA ATCCATGCTG TTGAAAAAAA CAGGATTGAT TTTGAAGAGT 
TCCATTGTGA ATCCAGGGAC AGAGTTGCTG TTTCAACTAA TTGAATCAAT TCGTCATAGT 
TCCAGTGTGA ATTCAGAGAC AAAAAAGATG CTGTCTGAAA TAACTCAATC AACTGTTCAA 
AGTTCCAGTG TGAATCCAGT GACAGAAGAG ATGATAGAGG AGAAATATCA ATTGCTTCTT 
CAAAGTTCCA GTGTGAATCT AGAAGCAGAA AATATACTGA ATGTTACAAC CCAATGGATT 
CTTCAAATTT CCAGTGTGAA TCAAGGAAAA GAAATGTTGC TCTTGGATAA AATTCAGCAG 
ATTCTTCAAA TTTCAAGTGT GAATCCAGAA ACAAAAGATT TGTTGTTGGA AAAAATTGAG 
TTGATTCTTC AAAATCCCGG TGTGCATCAA GAGACAAAAG ATTTGCTGTT GGAAAGAATT 
CAGCTGATCC TTCAAAATTC AAGTGTGCAA AAAGAGACAA AATATTTGCT GTTGGAAAGA 
ATTCGGTCGA TCCTTCAAAG TTCGTGTGTG CAAAAAGAGA CAAAAGATTT GCTGTTGGAA 
AGAATTCAGT CACTCCTTCA AAGTTCGTGT GTTCAAAAAG AGACAAAAGA TTTGTTGTTG 
GAAAAAATTC AGTTGATTCT TCAAAATTCC ACTGTGCATC AAGAAACAAA AGATTTGTTG 
TTGGAAAGAA TTCAGTTGTT TCTTCAAAAT TCCACTGTGG ATCAAGAGAC AAGTTATTTA 
CTCCTGAAAA GAATTCAGTT GATTCTTCAA AGTTCCAGTG TGAAAAAAGA GACAAAAGAG 
TTCGTGTTGT ATATAATTGA GTCGATTCTT CACAGTTCCA GTGTGCATCA AGAGACGAAA 
GATTTGCTGT TCGACAGAGT TCAGTTGATT CTTGAAAGTT CCAGTGTGCA GCAAGGGTTG 
AAATGTTTGC TGTTGAACAT GATTCAGGTG ATTTTTCAAA GGACAAGTGT GCAAAAAGAG 
ATGAAAGATT TGCTATTCAA CAGAATTCCG TTGATTCTTG AAAGTTCCAG TGTGCAGCAA 
GAGACAAAAG ATAAGCTGTT GGACATAATT CAGTCGATTC TTCAAAGGAC CAGTGTACAA 
GAAGAGACAA AAGATAAGCT GTTGGACATA ATTCAGTCGA TTCTTCAAAG AAGCAGTGTG 
CAAAAAGATA CGATAGATAC GCTGTTGGAC AGAATTCAGT TGATTCTTCA AAGAACCAGT 
GTGCAAAAAG AGATGAAAGA CTTCCTGCTG GACAGAATTA AGTCAATTCT TGAAAGTTTC 
AGTGTGCATC AAGAGACGAA AAAATTGCTG TTGAACAGAA TTATGTCGAT TCTTGAAAGT 
TCCACTGTGC ATCAAGAAAC GAAAAAATTG CTGTTGGAGA GAATTCAGTC GATTCTTGAA 
AGTTCCAGTG TGCAGCAAGA GACAAAAAAA TTGCTGTTGG ACAGAATTCA TTCGATTCTT 
AAAAGTTCCA GTGTGCAAAA AGAGACAAAA AACTTGCTGT TGGACAGAAT TCATTCGATT 
CTTAAAAGTT CCAGTGTGCA AAAAGAGTCC TGCAATAAGA GAAATCCTTT TTGGAAAAAG 
TATGCATTGG ACCTCGGAAT TTCAGTGTTC ACAATTATAG TAGTCACTCT CATTATGCAT 
TTAAAACAAA GAGAAGCAGA CCAACATTTT GAACTGAATA CTTTGTGGTC AAAAGATACT 
AGTGTGATCC TGTGTGTGCT GATCATGTTT AACAACAGGC TCAAGGCATT GATATACTTC 
AGACTGTATG GATTTTCACC TCCAGGTAAA GCACATAAGT ACATAGTAAA CTACATATTA 
AGATTCTCAC ATCCCGTCTT TTGCATTGTC TACTCTGCAA CAATCCTTTA TATGTATCTG 
CAAATACAGA ACAAAGATTC CTTATTTTCC TTGTATAATA GCTGGATGGT AGAAATGGAA 
ATGGTTCTAA TATTTCTGCT CGTAATTTTC AATGTTAAAA ACATTGCAAC TGTATTGTTA 
TACTTTGACA GTACAACTCT ACGACTATTC TTCTGGATTA AAGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109429.1
CDS	16..4443
Translation	MDKRADLGSTNSGVMGAVGIPLTAHCVVLFLLQMVALSSEQFTVNGLESPVLVPLDGNLELSCQLSPPQQAKHMEIRWFKNRYSEPVYLYRNGKDLFGEIVYKYVERTELLKDDIGKGKVTLRIFKVTSVDSGSYHCFFKDGKFYEEHIIDVKVTATSSDIQILMHPPNIKGVRLECHSGGWFPQPHMEWRDSKGEYIPATSKSHSQDENEFFNMTMDLFIKSNSHWSATCYIQNFVTHQEESISIVLPGVWDSWCPAWIMITLLIVIHMTYYIKLYRTYATKEMLLKSIQSSSQSSTVDTEKKEWLLKSIQSRIQSSSVDAEKKEWLLKFIQSSIQSSSVDLEKKEWLLKSIQSSIQSAIVDRGTKEWLLESIQSSILSSIVDQGTKVLLLKSIQSSIQSSTLDIETKELLLKSIQSSIQSSIMDLGTKELLLKIIQSSIQSSSVDLGTKELLLKIIQSSIQSSTVDLGTKELLLKIIQSSIQSSSVDLGTKELLLKIIQSSIQSSTVDLGTKELLLKIIQSSIQSSSVDLGAKMGLLESILSSIQSSNVDLETKVLLLKSIQSSIQSSTVYLGIKQWLLERIESIIQSSSVYLDTKELLLKIIQSSIESYSVDLGNKEFLLKIILSSIQSSSVDLGTKELLVKFFQSNIQSSSVIPGTKKLLVKIIQSSLQSSSVDLGTKKVLLDIIQTSIQNSNVHTERKGLLLKIIQSSVQSSSVDQGTKEMLLEIIQSSIQNSSVNQWTKDLLLKIIQSIIQSSSVDLGTKGFLLKIIQSSIQSSSVDIGTKSMLLKKTGLILKSSIVNPGTELLFQLIESIRHSSSVNSETKKMLSEITQSTVQSSSVNPVTEEMIEEKYQLLLQSSSVNLEAENILNVTTQWILQISSVNQGKEMLLLDKIQQILQISSVNPETKDLLLEKIELILQNPGVHQETKDLLLERIQLILQNSSVQKETKYLLLERIRSILQSSCVQKETKDLLLERIQSLLQSSCVQKETKDLLLEKIQLILQNSTVHQETKDLLLERIQLFLQNSTVDQETSYLLLKRIQLILQSSSVKKETKEFVLYIIESILHSSSVHQETKDLLFDRVQLILESSSVQQGLKCLLLNMIQVIFQRTSVQKEMKDLLFNRIPLILESSSVQQETKDKLLDIIQSILQRTSVQEETKDKLLDIIQSILQRSSVQKDTIDTLLDRIQLILQRTSVQKEMKDFLLDRIKSILESFSVHQETKKLLLNRIMSILESSTVHQETKKLLLERIQSILESSSVQQETKKLLLDRIHSILKSSSVQKETKNLLLDRIHSILKSSSVQKESCNKRNPFWKKYALDLGISVFTIIVVTLIMHLKQREADQHFELNTLWSKDTSVILCVLIMFNNRLKALIYFRLYGFSPPGKAHKYIVNYILRFSHPVFCIVYSATILYMYLQIQNKDSLFSLYNSWMVEMEMVLIFLLVIFNVKNIATVLLYFDSTTLRLFFWIKG

Target ORF information:

RefSeq Version	XM_030253569.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus selection and upkeep of intraepithelial T cells 5 (Skint5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253569.1

ORF Insert Sequence:

ATGGACAAGA GAGCTGACTT AGGATCAACA AACTCAGGTG TGATGGGAGC TGTAGGAATA 
CCTCTTACCG CACACTGCGT TGTTTTGTTT CTCCTCCAGA TGGTAGCACT GAGTTCAGAA 
CAATTCACTG TGAATGGCTT AGAGAGTCCA GTCTTGGTTC CATTGGATGG AAACCTAGAA 
CTCAGTTGTC AGTTGTCTCC ACCACAGCAA GCAAAACACA TGGAAATTCG CTGGTTCAAG 
AATCGCTATT CAGAGCCAGT ATACCTGTAT AGAAATGGTA AAGACCTTTT TGGAGAAATT 
GTCTACAAGT ATGTAGAGAG AACTGAACTC CTGAAAGATG ACATCGGGAA AGGGAAAGTA 
ACCCTCAGGA TCTTTAAAGT AACTAGTGTT GACAGTGGGT CCTACCACTG CTTCTTCAAA 
GATGGCAAGT TCTATGAAGA GCATATCATA GACGTCAAGG TCACAGCTAC AAGCTCAGAC 
ATACAGATTC TTATGCATCC CCCAAATATC AAAGGTGTGA GGTTGGAGTG TCATTCAGGC 
GGTTGGTTCC CACAGCCTCA CATGGAATGG AGAGATAGCA AAGGAGAGTA CATTCCAGCT 
ACATCAAAAT CCCATTCACA GGATGAAAAT GAATTTTTCA ACATGACAAT GGACCTTTTT 
ATTAAATCCA ACTCTCACTG GAGTGCAACC TGCTATATTC AAAACTTTGT AACTCACCAA 
GAAGAAAGCA TAAGTATTGT CCTACCAGGT GTGTGGGATT CATGGTGTCC TGCTTGGATA 
ATGATTACTT TGCTGATTGT CATCCACATG ACTTACTATA TTAAATTATA CCGCACATAT 
GCGACAAAAG AGATGCTTTT GAAAAGTATT CAATCAAGTT CTCAAAGTTC CACTGTGGAT 
ACAGAGAAAA AAGAGTGGCT GTTGAAAAGT ATTCAATCAA GGATTCAAAG TTCCAGTGTG 
GATGCAGAGA AAAAAGAGTG GCTGTTGAAA TTTATTCAAT CAAGTATTCA AAGTTCCAGT 
GTGGATCTAG AAAAAAAAGA GTGGCTGTTG AAAAGCATTC AATCAAGTAT TCAAAGTGCC 
ATTGTGGATC GAGGAACAAA AGAGTGGCTG TTGGAAAGCA TTCAATCAAG TATTCTAAGT 
TCCATTGTGG ATCAAGGGAC AAAAGTGTTG CTGTTGAAAA GTATTCAATC AAGCATTCAA 
AGTTCCACTC TGGATATAGA AACAAAGGAG TTGCTGTTGA AAAGCATTCA ATCAAGTATT 
CAAAGTTCCA TTATGGATCT AGGAACAAAA GAGTTGCTGT TGAAAATAAT TCAATCGAGT 
ATTCAAAGTT CCAGTGTGGA TCTAGGAACA AAAGAGTTGC TGTTGAAAAT AATTCAATCG 
AGTATTCAAA GTTCCACTGT GGATCTAGGA ACAAAAGAGT TGCTGTTGAA AATAATTCAA 
TCGAGTATTC AAAGTTCCAG TGTGGATCTA GGAACAAAAG AGTTGCTGTT GAAAATAATT 
CAATCGAGTA TTCAAAGTTC CACTGTGGAT CTAGGAACAA AAGAGTTGCT GTTGAAAATA 
ATTCAATCGA GTATTCAAAG TTCCAGTGTG GATCTAGGAG CAAAAATGGG GCTGTTGGAA 
AGCATTTTGT CAAGTATTCA AAGTTCCAAT GTGGATCTAG AGACAAAAGT GTTGCTGTTG 
AAAAGTATTC AATCAAGTAT TCAAAGTTCC ACTGTGTATC TAGGAATAAA ACAATGGCTG 
TTGGAAAGAA TTGAATCAAT TATTCAAAGT TCCAGTGTGT ATCTAGATAC AAAAGAGTTG 
CTGTTGAAAA TTATTCAATC AAGTATTGAA AGTTACAGTG TGGATCTAGG GAACAAAGAG 
TTTCTGTTGA AAATTATTCT ATCAAGTATC CAAAGTTCCA GTGTGGATCT AGGGACAAAA 
GAGTTGCTGG TGAAATTTTT TCAATCAAAT ATTCAAAGTT CCAGTGTGAT TCCAGGGACA 
AAAAAGTTGC TGGTGAAAAT TATTCAATCA AGTCTTCAAA GTTCCAGTGT GGATCTAGGG 
ACAAAAAAGG TGTTGTTGGA TATTATTCAA ACAAGTATTC AAAACTCCAA TGTGCATACA 
GAGAGAAAAG GGTTGCTGTT GAAAATTATT CAATCAAGTG TTCAAAGTTC CAGTGTGGAT 
CAAGGGACAA AAGAGATGCT GTTGGAAATT ATTCAATCAA GTATTCAAAA TTCCAGTGTG 
AATCAATGGA CAAAAGATTT GCTGCTGAAA ATTATTCAAT CAATTATTCA AAGTTCCAGT 
GTGGATCTAG GGACAAAAGG GTTCCTTTTG AAAATTATTC AATCAAGTAT TCAAAGTTCC 
AGTGTGGATA TAGGGACCAA ATCCATGCTG TTGAAAAAAA CAGGATTGAT TTTGAAGAGT 
TCCATTGTGA ATCCAGGGAC AGAGTTGCTG TTTCAACTAA TTGAATCAAT TCGTCATAGT 
TCCAGTGTGA ATTCAGAGAC AAAAAAGATG CTGTCTGAAA TAACTCAATC AACTGTTCAA 
AGTTCCAGTG TGAATCCAGT GACAGAAGAG ATGATAGAGG AGAAATATCA ATTGCTTCTT 
CAAAGTTCCA GTGTGAATCT AGAAGCAGAA AATATACTGA ATGTTACAAC CCAATGGATT 
CTTCAAATTT CCAGTGTGAA TCAAGGAAAA GAAATGTTGC TCTTGGATAA AATTCAGCAG 
ATTCTTCAAA TTTCAAGTGT GAATCCAGAA ACAAAAGATT TGTTGTTGGA AAAAATTGAG 
TTGATTCTTC AAAATCCCGG TGTGCATCAA GAGACAAAAG ATTTGCTGTT GGAAAGAATT 
CAGCTGATCC TTCAAAATTC AAGTGTGCAA AAAGAGACAA AATATTTGCT GTTGGAAAGA 
ATTCGGTCGA TCCTTCAAAG TTCGTGTGTG CAAAAAGAGA CAAAAGATTT GCTGTTGGAA 
AGAATTCAGT CACTCCTTCA AAGTTCGTGT GTTCAAAAAG AGACAAAAGA TTTGTTGTTG 
GAAAAAATTC AGTTGATTCT TCAAAATTCC ACTGTGCATC AAGAAACAAA AGATTTGTTG 
TTGGAAAGAA TTCAGTTGTT TCTTCAAAAT TCCACTGTGG ATCAAGAGAC AAGTTATTTA 
CTCCTGAAAA GAATTCAGTT GATTCTTCAA AGTTCCAGTG TGAAAAAAGA GACAAAAGAG 
TTCGTGTTGT ATATAATTGA GTCGATTCTT CACAGTTCCA GTGTGCATCA AGAGACGAAA 
GATTTGCTGT TCGACAGAGT TCAGTTGATT CTTGAAAGTT CCAGTGTGCA GCAAGGGTTG 
AAATGTTTGC TGTTGAACAT GATTCAGGTG ATTTTTCAAA GGACAAGTGT GCAAAAAGAG 
ATGAAAGATT TGCTATTCAA CAGAATTCCG TTGATTCTTG AAAGTTCCAG TGTGCAGCAA 
GAGACAAAAG ATAAGCTGTT GGACATAATT CAGTCGATTC TTCAAAGGAC CAGTGTACAA 
GAAGAGACAA AAGATAAGCT GTTGGACATA ATTCAGTCGA TTCTTCAAAG AAGCAGTGTG 
CAAAAAGATA CGATAGATAC GCTGTTGGAC AGAATTCAGT TGATTCTTCA AAGAACCAGT 
GTGCAAAAAG AGATGAAAGA CTTCCTGCTG GACAGAATTA AGTCAATTCT TGAAAGTTTC 
AGTGTGCATC AAGAGACGAA AAAATTGCTG TTGAACAGAA TTATGTCGAT TCTTGAAAGT 
TCCACTGTGC ATCAAGAAAC GAAAAAATTG CTGTTGGAGA GAATTCAGTC GATTCTTGAA 
AGTTCCAGTG TGCAGCAAGA GACAAAAAAA TTGCTGTTGG ACAGAATTCA TTCGATTCTT 
AAAAGTTCCA GTGTGCAAAA AGAGACAAAA AACTTGCTGT TGGACAGAAT TCATTCGATT 
CTTAAAAGTT CCAGTGTGCA AAAAGAGTCC TGCAATAAGA GAAATCCTTT TTGGAAAAAG 
TATGCATTGG ACCTCGGAAT TTCAGTGTTC ACAATTATAG TAGTCACTCT CATTATGCAT 
TTAAAACAAA GAGAAGCAGA CCAACATTTT GAACTGAATA CTTTGTGGTC AAAAGATACT 
AGTGTGATCC TGTGTGTGCT GATCATGTTT AACAACAGGC TCAAGGCATT GATATACTTC 
AGACTGTATG GATTTTCACC TCCAGGTAAA GCACATAAGT ACATAGTAAA CTACATATTA 
AGATTCTCAC ATCCCGTCTT TTGCATTGTC TACTCTGCAA CAATCCTTTA TATGTATCTG 
CAAATACAGA ACAAAGATTC CTTATTTTCC TTGTATAATA GCTGGATGGT AGAAATGGAA 
ATGGTTCTAA TATTTCTGCT CGTAATTTTC AATGTTAAAA ACATTGCAAC TGTATTGTTA 
TACTTTGACA GTACAACTCT ACGACTATTC TTCTGGATTA AAGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skint5 ( XM_030253570.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109430.1 Mus musculus selection and upkeep of intraepithelial T cells 5 (Skint5), transcript variant X2, mRNA.

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CloneID	OMu74113
Clone ID Related Accession (Same CDS sequence)	XM_030253570.1
Accession Version	XM_030253570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	selection and upkeep of intraepithelial T-cells protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGA GAGCTGACTT AGGATCAACA AACTCAGGTG TGATGGGAGC TGTAGGAATA 
CCTCTTACCG CACACTGCGT TGTTTTGTTT CTCCTCCAGA TGGTAGCACT GAGTTCAGAA 
CAATTCACTG TGAATGGCTT AGAGAGTCCA GTCTTGGTTC CATTGGATGG AAACCTAGAA 
CTCAGTTGTC AGTTGTCTCC ACCACAGCAA GCAAAACACA TGGAAATTCG CTGGTTCAAG 
AATCGCTATT CAGAGCCAGT ATACCTGTAT AGAAATGGTA AAGACCTTTT TGGAGAAATT 
GTCTACAAGT ATGTAGAGAG AACTGAACTC CTGAAAGATG ACATCGGGAA AGGGAAAGTA 
ACCCTCAGGA TCTTTAAAGT AACTAGTGTT GACAGTGGGT CCTACCACTG CTTCTTCAAA 
GATGGCAAGT TCTATGAAGA GCATATCATA GACGTCAAGG TCACAGCTAC AAGCTCAGAC 
ATACAGATTC TTATGCATCC CCCAAATATC AAAGGTGTGA GGTTGGAGTG TCATTCAGGC 
GGTTGGTTCC CACAGCCTCA CATGGAATGG AGAGATAGCA AAGGAGAGTA CATTCCAGCT 
ACATCAAAAT CCCATTCACA GGATGAAAAT GAATTTTTCA ACATGACAAT GGACCTTTTT 
ATTAAATCCA ACTCTCACTG GAGTGCAACC TGCTATATTC AAAACTTTGT AACTCACCAA 
GAAGAAAGCA TAAGTATTGT CCTACCAGGT GTGTGGGATT CATGGTGTCC TGCTTGGATA 
ATGATTACTT TGCTGATTGT CATCCACATG ACTTACTATA TTAAATTATA CCGCACATAT 
GCGACAAAAG AGATGCTTTT GAAAAGTATT CAATCAAGTT CTCAAAGTTC CACTGTGGAT 
ACAGAGAAAA AAGAGTGGCT GTTGAAAAGT ATTCAATCAA GGATTCAAAG TTCCAGTGTG 
GATGCAGAGA AAAAAGAGTG GCTGTTGAAA TTTATTCAAT CAAGTATTCA AAGTTCCAGT 
GTGGATCTAG AAAAAAAAGA GTGGCTGTTG AAAAGCATTC AATCAAGTAT TCAAAGTGCC 
ATTGTGGATC GAGGAACAAA AGAGTGGCTG TTGGAAAGCA TTCAATCAAG TATTCTAAGT 
TCCATTGTGG ATCAAGGGAC AAAAGTGTTG CTGTTGAAAA GTATTCAATC AAGCATTCAA 
AGTTCCACTC TGGATATAGA AACAAAGGAG TTGCTGTTGA AAAGCATTCA ATCAAGTATT 
CAAAGTTCCA TTATGGATCT AGGAACAAAA GAGTTGCTGT TGAAAATAAT TCAATCGAGT 
ATTCAAAGTT CCAGTGTGGA TCTAGGAACA AAAGAGTTGC TGTTGAAAAT AATTCAATCG 
AGTATTCAAA GTTCCACTGT GGATCTAGGA ACAAAAGAGT TGCTGTTGAA AATAATTCAA 
TCGAGTATTC AAAGTTCCAG TGTGGATCTA GGAACAAAAG AGTTGCTGTT GAAAATAATT 
CAATCGAGTA TTCAAAGTTC CACTGTGGAT CTAGGAACAA AAGAGTTGCT GTTGAAAATA 
ATTCAATCGA GTATTCAAAG TTCCAGTGTG GATCTAGGAG CAAAAATGGG GCTGTTGGAA 
AGCATTTTGT CAAGTATTCA AAGTTCCAAT GTGGATCTAG AGACAAAAGT GTTGCTGTTG 
AAAAGTATTC AATCAAGTAT TCAAAGTTCC ACTGTGTATC TAGGAATAAA ACAATGGCTG 
TTGGAAAGAA TTGAATCAAT TATTCAAAGT TCCAGTGTGT ATCTAGATAC AAAAGAGTTG 
CTGTTGAAAA TTATTCAATC AAGTATTGAA AGTTACAGTG TGGATCTAGG GAACAAAGAG 
TTTCTGTTGA AAATTATTCT ATCAAGTATC CAAAGTTCCA GTGTGGATCT AGGGACAAAA 
GAGTTGCTGG TGAAATTTTT TCAATCAAAT ATTCAAAGTT CCAGTGTGAT TCCAGGGACA 
AAAAAGTTGC TGGTGAAAAT TATTCAATCA AGTCTTCAAA GTTCCAGTGT GGATCTAGGG 
ACAAAAAAGG TGTTGTTGGA TATTATTCAA ACAAGTATTC AAAACTCCAA TGTGCATACA 
GAGAGAAAAG GGTTGCTGTT GAAAATTATT CAATCAAGTG TTCAAAGTTC CAGTGTGGAT 
CAAGGGACAA AAGAGATGCT GTTGGAAATT ATTCAATCAA GTATTCAAAA TTCCAGTGTG 
AATCAATGGA CAAAAGATTT GCTGCTGAAA ATTATTCAAT CAATTATTCA AAGTTCCAGT 
GTGGATCTAG GGACAAAAGG GTTCCTTTTG AAAATTATTC AATCAAGTAT TCAAAGTTCC 
AGTGTGGATA TAGGGACCAA ATCCATGCTG TTGAAAAAAA CAGGATTGAT TTTGAAGAGT 
TCCATTGTGA ATCCAGGGAC AGAGTTGCTG TTTCAACTAA TTGAATCAAT TCGTCATAGT 
TCCAGTGTGA ATTCAGAGAC AAAAAAGATG CTGTCTGAAA TAACTCAATC AACTGTTCAA 
AGTTCCAGTG TGAATCCAGT GACAGAAGAG ATGATAGAGG AGAAATATCA ATTGCTTCTT 
CAAAGTTCCA GTGTGAATCT AGAAGCAGAA AATATACTGA ATGTTACAAC CCAATGGATT 
CTTCAAATTT CCAGTGTGAA TCAAGGAAAA GAAATGTTGC TCTTGGATAA AATTCAGCAG 
ATTCTTCAAA TTTCAAGTGT GAATCCAGAA ACAAAAGATT TGTTGTTGGA AAAAATTGAG 
TTGATTCTTC AAAATCCCGG TGTGCATCAA GAGACAAAAG ATTTGCTGTT GGAAAGAATT 
CAGCTGATCC TTCAAAATTC AAGTGTGCAA AAAGAGACAA AATATTTGCT GTTGGAAAGA 
ATTCGGTCGA TCCTTCAAAG TTCGTGTGTG CAAAAAGAGA CAAAAGATTT GCTGTTGGAA 
AGAATTCAGT CACTCCTTCA AAGTTCGTGT GTTCAAAAAG AGACAAAAGA TTTGTTGTTG 
GAAAAAATTC AGTTGATTCT TCAAAATTCC ACTGTGCATC AAGAAACAAA AGATTTGTTG 
TTGGAAAGAA TTCAGTTGTT TCTTCAAAAT TCCACTGTGG ATCAAGAGAC AAGTTATTTA 
CTCCTGAAAA GAATTCAGTT GATTCTTCAA AGTTCCAGTG TGAAAAAAGA GACAAAAGAG 
TTCGTGTTGT ATATAATTGA GTCGATTCTT CACAGTTCCA GTGTGCATCA AGAGACGAAA 
GATTTGCTGT TCGACAGAGT TCAGTTGATT CTTGAAAGTT CCAGTGTGCA GCAAGGGTTG 
AAATGTTTGC TGTTGAACAT GATTCAGGTG ATTTTTCAAA GGACAAGTGT GCAAAAAGAG 
ATGAAAGATT TGCTATTCAA CAGAATTCCG TTGATTCTTG AAAGTTCCAG TGTGCAGCAA 
GAGACAAAAG ATAAGCTGTT GGACATAATT CAGTCGATTC TTCAAAGGAC CAGTGTACAA 
GAAGAGACAA AAGATAAGCT GTTGGACATA ATTCAGTCGA TTCTTCAAAG AAGCAGTGTG 
CAAAAAGATA CGATAGATAC GCTGTTGGAC AGAATTCAGT TGATTCTTCA AAGAACCAGT 
GTGCAAAAAG AGATGAAAGA CTTCCTGCTG GACAGAATTA AGTCAATTCT TGAAAGTTTC 
AGTGTGCATC AAGAGACGAA AAAATTGCTG TTGAACAGAA TTATGTCGAT TCTTGAAAGT 
TCCACTGTGC ATCAAGAGAC AAAAAAATTG CTGTTGGACA GAATTCATTC GATTCTTAAA 
AGTTCCAGTG TGCAAAAAGA GACAAAAAAC TTGCTGTTGG ACAGAATTCA TTCGATTCTT 
AAAAGTTCCA GTGTGCAAAA AGAGTCCTGC AATAAGAGAA ATCCTTTTTG GAAAAAGTAT 
GCATTGGACC TCGGAATTTC AGTGTTCACA ATTATAGTAG TCACTCTCAT TATGCATTTA 
AAACAAAGAG AAGCAGACCA ACATTTTGAA CTGAATACTT TGTGGTCAAA AGATACTAGT 
GTGATCCTGT GTGTGCTGAT CATGTTTAAC AACAGGCTCA AGGCATTGAT ATACTTCAGA 
CTGTATGGAT TTTCACCTCC AGGTAAAGCA CATAAGTACA TAGTAAACTA CATATTAAGA 
TTCTCACATC CCGTCTTTTG CATTGTCTAC TCTGCAACAA TCCTTTATAT GTATCTGCAA 
ATACAGAACA AAGATTCCTT ATTTTCCTTG TATAATAGCT GGATGGTAGA AATGGAAATG 
GTTCTAATAT TTCTGCTCGT AATTTTCAAT GTTAAAAACA TTGCAACTGT ATTGTTATAC 
TTTGACAGTA CAACTCTACG ACTATTCTTC TGGATTAAAG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109430.1
CDS	16..4380
Translation	MDKRADLGSTNSGVMGAVGIPLTAHCVVLFLLQMVALSSEQFTVNGLESPVLVPLDGNLELSCQLSPPQQAKHMEIRWFKNRYSEPVYLYRNGKDLFGEIVYKYVERTELLKDDIGKGKVTLRIFKVTSVDSGSYHCFFKDGKFYEEHIIDVKVTATSSDIQILMHPPNIKGVRLECHSGGWFPQPHMEWRDSKGEYIPATSKSHSQDENEFFNMTMDLFIKSNSHWSATCYIQNFVTHQEESISIVLPGVWDSWCPAWIMITLLIVIHMTYYIKLYRTYATKEMLLKSIQSSSQSSTVDTEKKEWLLKSIQSRIQSSSVDAEKKEWLLKFIQSSIQSSSVDLEKKEWLLKSIQSSIQSAIVDRGTKEWLLESIQSSILSSIVDQGTKVLLLKSIQSSIQSSTLDIETKELLLKSIQSSIQSSIMDLGTKELLLKIIQSSIQSSSVDLGTKELLLKIIQSSIQSSTVDLGTKELLLKIIQSSIQSSSVDLGTKELLLKIIQSSIQSSTVDLGTKELLLKIIQSSIQSSSVDLGAKMGLLESILSSIQSSNVDLETKVLLLKSIQSSIQSSTVYLGIKQWLLERIESIIQSSSVYLDTKELLLKIIQSSIESYSVDLGNKEFLLKIILSSIQSSSVDLGTKELLVKFFQSNIQSSSVIPGTKKLLVKIIQSSLQSSSVDLGTKKVLLDIIQTSIQNSNVHTERKGLLLKIIQSSVQSSSVDQGTKEMLLEIIQSSIQNSSVNQWTKDLLLKIIQSIIQSSSVDLGTKGFLLKIIQSSIQSSSVDIGTKSMLLKKTGLILKSSIVNPGTELLFQLIESIRHSSSVNSETKKMLSEITQSTVQSSSVNPVTEEMIEEKYQLLLQSSSVNLEAENILNVTTQWILQISSVNQGKEMLLLDKIQQILQISSVNPETKDLLLEKIELILQNPGVHQETKDLLLERIQLILQNSSVQKETKYLLLERIRSILQSSCVQKETKDLLLERIQSLLQSSCVQKETKDLLLEKIQLILQNSTVHQETKDLLLERIQLFLQNSTVDQETSYLLLKRIQLILQSSSVKKETKEFVLYIIESILHSSSVHQETKDLLFDRVQLILESSSVQQGLKCLLLNMIQVIFQRTSVQKEMKDLLFNRIPLILESSSVQQETKDKLLDIIQSILQRTSVQEETKDKLLDIIQSILQRSSVQKDTIDTLLDRIQLILQRTSVQKEMKDFLLDRIKSILESFSVHQETKKLLLNRIMSILESSTVHQETKKLLLDRIHSILKSSSVQKETKNLLLDRIHSILKSSSVQKESCNKRNPFWKKYALDLGISVFTIIVVTLIMHLKQREADQHFELNTLWSKDTSVILCVLIMFNNRLKALIYFRLYGFSPPGKAHKYIVNYILRFSHPVFCIVYSATILYMYLQIQNKDSLFSLYNSWMVEMEMVLIFLLVIFNVKNIATVLLYFDSTTLRLFFWIKG

Target ORF information:

RefSeq Version	XM_030253570.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus selection and upkeep of intraepithelial T cells 5 (Skint5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253570.1

ORF Insert Sequence:

ATGGACAAGA GAGCTGACTT AGGATCAACA AACTCAGGTG TGATGGGAGC TGTAGGAATA 
CCTCTTACCG CACACTGCGT TGTTTTGTTT CTCCTCCAGA TGGTAGCACT GAGTTCAGAA 
CAATTCACTG TGAATGGCTT AGAGAGTCCA GTCTTGGTTC CATTGGATGG AAACCTAGAA 
CTCAGTTGTC AGTTGTCTCC ACCACAGCAA GCAAAACACA TGGAAATTCG CTGGTTCAAG 
AATCGCTATT CAGAGCCAGT ATACCTGTAT AGAAATGGTA AAGACCTTTT TGGAGAAATT 
GTCTACAAGT ATGTAGAGAG AACTGAACTC CTGAAAGATG ACATCGGGAA AGGGAAAGTA 
ACCCTCAGGA TCTTTAAAGT AACTAGTGTT GACAGTGGGT CCTACCACTG CTTCTTCAAA 
GATGGCAAGT TCTATGAAGA GCATATCATA GACGTCAAGG TCACAGCTAC AAGCTCAGAC 
ATACAGATTC TTATGCATCC CCCAAATATC AAAGGTGTGA GGTTGGAGTG TCATTCAGGC 
GGTTGGTTCC CACAGCCTCA CATGGAATGG AGAGATAGCA AAGGAGAGTA CATTCCAGCT 
ACATCAAAAT CCCATTCACA GGATGAAAAT GAATTTTTCA ACATGACAAT GGACCTTTTT 
ATTAAATCCA ACTCTCACTG GAGTGCAACC TGCTATATTC AAAACTTTGT AACTCACCAA 
GAAGAAAGCA TAAGTATTGT CCTACCAGGT GTGTGGGATT CATGGTGTCC TGCTTGGATA 
ATGATTACTT TGCTGATTGT CATCCACATG ACTTACTATA TTAAATTATA CCGCACATAT 
GCGACAAAAG AGATGCTTTT GAAAAGTATT CAATCAAGTT CTCAAAGTTC CACTGTGGAT 
ACAGAGAAAA AAGAGTGGCT GTTGAAAAGT ATTCAATCAA GGATTCAAAG TTCCAGTGTG 
GATGCAGAGA AAAAAGAGTG GCTGTTGAAA TTTATTCAAT CAAGTATTCA AAGTTCCAGT 
GTGGATCTAG AAAAAAAAGA GTGGCTGTTG AAAAGCATTC AATCAAGTAT TCAAAGTGCC 
ATTGTGGATC GAGGAACAAA AGAGTGGCTG TTGGAAAGCA TTCAATCAAG TATTCTAAGT 
TCCATTGTGG ATCAAGGGAC AAAAGTGTTG CTGTTGAAAA GTATTCAATC AAGCATTCAA 
AGTTCCACTC TGGATATAGA AACAAAGGAG TTGCTGTTGA AAAGCATTCA ATCAAGTATT 
CAAAGTTCCA TTATGGATCT AGGAACAAAA GAGTTGCTGT TGAAAATAAT TCAATCGAGT 
ATTCAAAGTT CCAGTGTGGA TCTAGGAACA AAAGAGTTGC TGTTGAAAAT AATTCAATCG 
AGTATTCAAA GTTCCACTGT GGATCTAGGA ACAAAAGAGT TGCTGTTGAA AATAATTCAA 
TCGAGTATTC AAAGTTCCAG TGTGGATCTA GGAACAAAAG AGTTGCTGTT GAAAATAATT 
CAATCGAGTA TTCAAAGTTC CACTGTGGAT CTAGGAACAA AAGAGTTGCT GTTGAAAATA 
ATTCAATCGA GTATTCAAAG TTCCAGTGTG GATCTAGGAG CAAAAATGGG GCTGTTGGAA 
AGCATTTTGT CAAGTATTCA AAGTTCCAAT GTGGATCTAG AGACAAAAGT GTTGCTGTTG 
AAAAGTATTC AATCAAGTAT TCAAAGTTCC ACTGTGTATC TAGGAATAAA ACAATGGCTG 
TTGGAAAGAA TTGAATCAAT TATTCAAAGT TCCAGTGTGT ATCTAGATAC AAAAGAGTTG 
CTGTTGAAAA TTATTCAATC AAGTATTGAA AGTTACAGTG TGGATCTAGG GAACAAAGAG 
TTTCTGTTGA AAATTATTCT ATCAAGTATC CAAAGTTCCA GTGTGGATCT AGGGACAAAA 
GAGTTGCTGG TGAAATTTTT TCAATCAAAT ATTCAAAGTT CCAGTGTGAT TCCAGGGACA 
AAAAAGTTGC TGGTGAAAAT TATTCAATCA AGTCTTCAAA GTTCCAGTGT GGATCTAGGG 
ACAAAAAAGG TGTTGTTGGA TATTATTCAA ACAAGTATTC AAAACTCCAA TGTGCATACA 
GAGAGAAAAG GGTTGCTGTT GAAAATTATT CAATCAAGTG TTCAAAGTTC CAGTGTGGAT 
CAAGGGACAA AAGAGATGCT GTTGGAAATT ATTCAATCAA GTATTCAAAA TTCCAGTGTG 
AATCAATGGA CAAAAGATTT GCTGCTGAAA ATTATTCAAT CAATTATTCA AAGTTCCAGT 
GTGGATCTAG GGACAAAAGG GTTCCTTTTG AAAATTATTC AATCAAGTAT TCAAAGTTCC 
AGTGTGGATA TAGGGACCAA ATCCATGCTG TTGAAAAAAA CAGGATTGAT TTTGAAGAGT 
TCCATTGTGA ATCCAGGGAC AGAGTTGCTG TTTCAACTAA TTGAATCAAT TCGTCATAGT 
TCCAGTGTGA ATTCAGAGAC AAAAAAGATG CTGTCTGAAA TAACTCAATC AACTGTTCAA 
AGTTCCAGTG TGAATCCAGT GACAGAAGAG ATGATAGAGG AGAAATATCA ATTGCTTCTT 
CAAAGTTCCA GTGTGAATCT AGAAGCAGAA AATATACTGA ATGTTACAAC CCAATGGATT 
CTTCAAATTT CCAGTGTGAA TCAAGGAAAA GAAATGTTGC TCTTGGATAA AATTCAGCAG 
ATTCTTCAAA TTTCAAGTGT GAATCCAGAA ACAAAAGATT TGTTGTTGGA AAAAATTGAG 
TTGATTCTTC AAAATCCCGG TGTGCATCAA GAGACAAAAG ATTTGCTGTT GGAAAGAATT 
CAGCTGATCC TTCAAAATTC AAGTGTGCAA AAAGAGACAA AATATTTGCT GTTGGAAAGA 
ATTCGGTCGA TCCTTCAAAG TTCGTGTGTG CAAAAAGAGA CAAAAGATTT GCTGTTGGAA 
AGAATTCAGT CACTCCTTCA AAGTTCGTGT GTTCAAAAAG AGACAAAAGA TTTGTTGTTG 
GAAAAAATTC AGTTGATTCT TCAAAATTCC ACTGTGCATC AAGAAACAAA AGATTTGTTG 
TTGGAAAGAA TTCAGTTGTT TCTTCAAAAT TCCACTGTGG ATCAAGAGAC AAGTTATTTA 
CTCCTGAAAA GAATTCAGTT GATTCTTCAA AGTTCCAGTG TGAAAAAAGA GACAAAAGAG 
TTCGTGTTGT ATATAATTGA GTCGATTCTT CACAGTTCCA GTGTGCATCA AGAGACGAAA 
GATTTGCTGT TCGACAGAGT TCAGTTGATT CTTGAAAGTT CCAGTGTGCA GCAAGGGTTG 
AAATGTTTGC TGTTGAACAT GATTCAGGTG ATTTTTCAAA GGACAAGTGT GCAAAAAGAG 
ATGAAAGATT TGCTATTCAA CAGAATTCCG TTGATTCTTG AAAGTTCCAG TGTGCAGCAA 
GAGACAAAAG ATAAGCTGTT GGACATAATT CAGTCGATTC TTCAAAGGAC CAGTGTACAA 
GAAGAGACAA AAGATAAGCT GTTGGACATA ATTCAGTCGA TTCTTCAAAG AAGCAGTGTG 
CAAAAAGATA CGATAGATAC GCTGTTGGAC AGAATTCAGT TGATTCTTCA AAGAACCAGT 
GTGCAAAAAG AGATGAAAGA CTTCCTGCTG GACAGAATTA AGTCAATTCT TGAAAGTTTC 
AGTGTGCATC AAGAGACGAA AAAATTGCTG TTGAACAGAA TTATGTCGAT TCTTGAAAGT 
TCCACTGTGC ATCAAGAGAC AAAAAAATTG CTGTTGGACA GAATTCATTC GATTCTTAAA 
AGTTCCAGTG TGCAAAAAGA GACAAAAAAC TTGCTGTTGG ACAGAATTCA TTCGATTCTT 
AAAAGTTCCA GTGTGCAAAA AGAGTCCTGC AATAAGAGAA ATCCTTTTTG GAAAAAGTAT 
GCATTGGACC TCGGAATTTC AGTGTTCACA ATTATAGTAG TCACTCTCAT TATGCATTTA 
AAACAAAGAG AAGCAGACCA ACATTTTGAA CTGAATACTT TGTGGTCAAA AGATACTAGT 
GTGATCCTGT GTGTGCTGAT CATGTTTAAC AACAGGCTCA AGGCATTGAT ATACTTCAGA 
CTGTATGGAT TTTCACCTCC AGGTAAAGCA CATAAGTACA TAGTAAACTA CATATTAAGA 
TTCTCACATC CCGTCTTTTG CATTGTCTAC TCTGCAACAA TCCTTTATAT GTATCTGCAA 
ATACAGAACA AAGATTCCTT ATTTTCCTTG TATAATAGCT GGATGGTAGA AATGGAAATG 
GTTCTAATAT TTCTGCTCGT AATTTTCAAT GTTAAAAACA TTGCAACTGT ATTGTTATAC 
TTTGACAGTA CAACTCTACG ACTATTCTTC TGGATTAAAG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Skint1, the prototype of a newly identified immunoglobulin superfamily gene cluster, positively selects epidermal gammadelta T cells.
Nature genetics40(5)656-62(2008 May)
Boyden LM,Lewis JM,Barbee SD,Bas A,Girardi M,Hayday AC,Tigelaar RE,Lifton RP

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Skint5 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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