Pde5a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde5a
Entrez Gene ID	242202
Full Name	phosphodiesterase 5A, cGMP-specific
Synonyms	Cgbpde,Cn5n,PDE5a2,Pde5,Pde5a1
General protein information	Preferred Names phosphodiesterase 5A, cGMP-specific Names cGMP-specific 3',5'-cyclic phosphodiesterase cGMP-binding cGMP-specific phosphodiesterase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 G1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006501481.2	XP_006501544.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
XM_006501481.3	XP_006501544.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
NM_153422.2	NP_700471.2	cGMP-specific 3',5'-cyclic phosphodiesterase

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pde5ab	NP_001116732.1
Drosophila melanogaster (fruit fly)	Pde11	NP_001188837.1
Pan troglodytes (chimpanzee)	PDE5A	XP_001150967.2
Macaca mulatta (Rhesus monkey)	PDE5A	XP_001099652.1
Canis lupus familiaris (dog)	PDE5A	NP_001003188.1
Bos taurus (cattle)	PDE5A	NP_776842.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008646	XP_314743.3
Rattus norvegicus (Norway rat)	Pde5a	NP_598268.1
Xenopus tropicalis (tropical clawed frog)	pde5a	NP_001072812.1
Homo sapiens (human)	PDE5A	NP_001074.2
Mus musculus (house mouse)	Pde5a	NP_700471.2
Gallus gallus (chicken)	PDE5A	XP_420631.3

Gene Ontology
Bibliography

Related articles in PubMed

Dysregulated NO/PDE5 signaling in the sickle cell mouse lower urinary tract: Reversal by oral nitrate therapy.
Musicki B, Anele UA, Campbell JD, Karakus S, Shiva S, Silva FH, Burnett AL
Life sciences238116922(2019 Dec)

Chronic phosphodiesterase type 5 inhibition has beneficial effects on subcutaneous adipose tissue plasticity in type 2 diabetic mice.
Fiore D, Gianfrilli D, Cardarelli S, Naro F, Lenzi A, Isidori AM, Venneri MA
Journal of cellular physiology233(11)8411-8417(2018 11)

MicroRNA-19a/b-3p protect the heart from hypertension-induced pathological cardiac hypertrophy through PDE5A.
Liu K, Hao Q, Wei J, Li GH, Wu Y, Zhao YF
Journal of hypertension36(9)1847-1857(2018 09)

Luteinizing hormone signaling phosphorylates and activates the cyclic GMP phosphodiesterase PDE5 in mouse ovarian follicles, contributing an additional component to the hormonally induced decrease in cyclic GMP that reinitiates meiosis.
Egbert JR, Yee SP, Jaffe LA
Developmental biology435(1)6-14(2018 03)

Transcriptional Architecture of Synaptic Communication Delineates GABAergic Neuron Identity.
Paul A, Crow M, Raudales R, He M, Gillis J, Huang ZJ
Cell171(3)522-539.e20(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pde5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02758	NM_153422.2 Latest version!	Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu31908	XM_006501481.3 Latest version!	Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu31908	XM_006501481.2	Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde5a ( NM_153422.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_700471.2 Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), mRNA.

Price & Availability↑

CloneID	OMu02758
Clone ID Related Accession (Same CDS sequence)	NM_153422.2
Accession Version	NM_153422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Mus musculus(house mouse)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC101922.12, BC119137.1, AC155158.15 and BX631325.1. On Oct 1, 2009 this sequence version replaced NM_153422.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF541937.1, BC119137.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGAACGAG CGGGCCCCAA CTCCGTGCGG TCGCAGCAGC AGCGGGACCC GGACTGGGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTT ACCTTCTCTT ACTTTATTAG AAAGGCCACC 
AGAGACATGG TCAACGCATG GTTTTCAGAG AGAGTTCACA ACATCCCTGT GTGCAAGGAA 
GGCATCCGAG CCCATACCGA GTCCTGCTCT TGCTCTCTGC AGCAAAGTCC TCATGCAGAT 
AATACCACCC CTGGAGCACC AGCCAGAAAA ATATCTGCCT CTGAATTTGA CCGACCTCTT 
AGACCCATTG TTGTCAAGGA TTCCGAGGGA ACAGTGAGCT TTCTCTCTGA CTCAGGAAAA 
AAGGAACAAA TGCCACTAAC CCCCCCAAGA TTTGATAGTG ATGAAGGGGA CCAGTGCTCA 
AGGCTCTTGG AGCTAGTAAA AGATATTTCC AGTCATTTGG ATGTCACAGC ATTGTGTCAC 
AAAATTTTCT TGCATATCCA TGGACTCATC TCTGCGGATC GCTATTCCCT GTTCCTTGTC 
TGTGAGGACA GCTCTAAAGA CAAATTCCTC ATCAGCCGCC TCTTTGATGT TGCTGAAGGT 
TCAACATTGG AGGAAGCTTC AAATAACTGT ATCCGTTTAG AGTGGAACAA AGGCATTGTG 
GGACATGTGG CAGCTTTTGG CGAGCCCTTG AACATCAAAG ATGCCTATGA GGATCCCCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGC TACAAGACAC AAAGTATCCT TTGTATGCCA 
ATTAAGAACC ACAGGGAAGA GGTTGTTGGT GTAGCTCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CCTTCACTGA AAAAGATGAA AAGGACTTTG CGGCCTACCT GGCATTCTGT 
GGTATTGTTC TTCACAATGC TCAGCTCTAT GAAACGTCAT TGCTGGAGAA CAAAAGAAAT 
CAGGTGTTAC TTGACCTTGC CAGCTTAATC TTTGAAGAGC AGCAGTCACT GGAAGTCATT 
CTGAAGAAGA TAGCGGCCAC TATCATCTCC TTCATGCAGG TGCAGAAGTG CACCATCTTC 
ATTGTGGATG AAGACTGTCC TGACTCTTTC TCTCGTGTGT TTCACATGGA GTGTGAGGAA 
GTAGGAAAAC CCTCTGATCC TTTAACGAGG GAGCAAGATG CAAACAAAAT CAACTACATG 
TATGCTCAGT ATGTCAAAAA CACGATGGAG CCACTTAATA TCCCAGATGT CACCAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACATG GGACACGTGA ACACGCCCTG CATTGGAAGT 
TTGCTTTGCA CCCCTATAAA AAATGGGAAG AAGAACAAAG TCATAGGCGT CTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG ATTAAAGCTT TCAACCAAAA TGATGAGCAG 
TTCCTGGAAG CCTTTGTCAT CTTCTGTGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCGGTGGAGA GAGCCATGGC CAAGCAGATG GTGACATTAG AGGTCCTGTC TTACCATGCA 
TCAGCGGCGG AGGAAGAAAC AAGAGAGCTC CAGGCTTTAT CGGCTGCTGT GGTACCATCG 
GCCCAAACCC TTAAAATTAC CGACTTCAGC TTCAGTGACT TTGAGCTGTC TGATCTGGAA 
ACAGCGCTGT GTACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTGAAGA AGAATTATCG GAAGAACGTT 
GCCTACCACA ATTGGAGGCA TGCCTTTAAC ACGGCACAGT GCATGTTTGC TGCTCTGAAA 
GCCGGCAAGA TCCAGAACAA GTTGACGGAT CTGGAGACCC TTGCGTTGCT CATTGCTGCT 
TTAAGCCATG ACTTGGATCA TCGTGGCGTG AACAACTCAT ACATACAGCG GAGCGAGCAC 
CCGCTGGCTC AGCTGTACTG TCACTCCATC ATGGAACACC ATCATTTTGA CCAGTGCTTG 
ATGATTCTAA ACAGCCCAGG CAACCAGATT CTCAGTGGCC TCTCCATTGA CGAATATAAG 
ACCACACTGA AAATAATCAA GCAAGCAATT TTAGCCACAG ACCTAGCACT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATACGA AAAAATCAAT TCAGTTTTGA AGATCCTCTT 
CAAAAGGAGT TGTTTCTAGC AATGCTGATG ACAGCCTGCG ACCTCTCTGC GATTACTAAA 
CCCTGGCCTA TTCAACAACG GATAGCAGAA CTCGTGGCAG CTGAATTCTT TGATCAAGGA 
GACAGAGAGA GAAAAGAACT TAACATGGAG CCGGCTGATC TAATGAACAG AGAGAAGAAA 
AATAAAATCC CAAGTATGCA AGTTGGGTTC ATAGATGCCA TCTGCTTGCA GCTGTATGAG 
GCCCTGACCC ACGTATCCGA AGACTGTTTG CCCTTGCTGG ATGGCTGCAG GAAGAACAGA 
CAGAAATGGC AGGCCCTCGC GGAGCAGCAG GAGAAGATGC TCCTTAACGG GGAGAGCAGC 
CAGGGCAAGC GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_700471.2
CDS	89..2686
Translation	MERAGPNSVRSQQQRDPDWVEAWLDDHRDFTFSYFIRKATRDMVNAWFSERVHNIPVCKEGIRAHTESCSCSLQQSPHADNTTPGAPARKISASEFDRPLRPIVVKDSEGTVSFLSDSGKKEQMPLTPPRFDSDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCPDSFSRVFHMECEEVGKPSDPLTREQDANKINYMYAQYVKNTMEPLNIPDVTKDKRFPWTNENMGHVNTPCIGSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKIKAFNQNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQALSAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIDEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFSFEDPLQKELFLAMLMTACDLSAITKPWPIQQRIAELVAAEFFDQGDRERKELNMEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCLPLLDGCRKNRQKWQALAEQQEKMLLNGESSQGKRD

Target ORF information:

RefSeq Version	NM_153422.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153422.2

ORF Insert Sequence:

ATGGAACGAG CGGGCCCCAA CTCCGTGCGG TCGCAGCAGC AGCGGGACCC GGACTGGGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTT ACCTTCTCTT ACTTTATTAG AAAGGCCACC 
AGAGACATGG TCAACGCATG GTTTTCAGAG AGAGTTCACA ACATCCCTGT GTGCAAGGAA 
GGCATCCGAG CCCATACCGA GTCCTGCTCT TGCTCTCTGC AGCAAAGTCC TCATGCAGAT 
AATACCACCC CTGGAGCACC AGCCAGAAAA ATATCTGCCT CTGAATTTGA CCGACCTCTT 
AGACCCATTG TTGTCAAGGA TTCCGAGGGA ACAGTGAGCT TTCTCTCTGA CTCAGGAAAA 
AAGGAACAAA TGCCACTAAC CCCCCCAAGA TTTGATAGTG ATGAAGGGGA CCAGTGCTCA 
AGGCTCTTGG AGCTAGTAAA AGATATTTCC AGTCATTTGG ATGTCACAGC ATTGTGTCAC 
AAAATTTTCT TGCATATCCA TGGACTCATC TCTGCGGATC GCTATTCCCT GTTCCTTGTC 
TGTGAGGACA GCTCTAAAGA CAAATTCCTC ATCAGCCGCC TCTTTGATGT TGCTGAAGGT 
TCAACATTGG AGGAAGCTTC AAATAACTGT ATCCGTTTAG AGTGGAACAA AGGCATTGTG 
GGACATGTGG CAGCTTTTGG CGAGCCCTTG AACATCAAAG ATGCCTATGA GGATCCCCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGC TACAAGACAC AAAGTATCCT TTGTATGCCA 
ATTAAGAACC ACAGGGAAGA GGTTGTTGGT GTAGCTCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CCTTCACTGA AAAAGATGAA AAGGACTTTG CGGCCTACCT GGCATTCTGT 
GGTATTGTTC TTCACAATGC TCAGCTCTAT GAAACGTCAT TGCTGGAGAA CAAAAGAAAT 
CAGGTGTTAC TTGACCTTGC CAGCTTAATC TTTGAAGAGC AGCAGTCACT GGAAGTCATT 
CTGAAGAAGA TAGCGGCCAC TATCATCTCC TTCATGCAGG TGCAGAAGTG CACCATCTTC 
ATTGTGGATG AAGACTGTCC TGACTCTTTC TCTCGTGTGT TTCACATGGA GTGTGAGGAA 
GTAGGAAAAC CCTCTGATCC TTTAACGAGG GAGCAAGATG CAAACAAAAT CAACTACATG 
TATGCTCAGT ATGTCAAAAA CACGATGGAG CCACTTAATA TCCCAGATGT CACCAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACATG GGACACGTGA ACACGCCCTG CATTGGAAGT 
TTGCTTTGCA CCCCTATAAA AAATGGGAAG AAGAACAAAG TCATAGGCGT CTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG ATTAAAGCTT TCAACCAAAA TGATGAGCAG 
TTCCTGGAAG CCTTTGTCAT CTTCTGTGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCGGTGGAGA GAGCCATGGC CAAGCAGATG GTGACATTAG AGGTCCTGTC TTACCATGCA 
TCAGCGGCGG AGGAAGAAAC AAGAGAGCTC CAGGCTTTAT CGGCTGCTGT GGTACCATCG 
GCCCAAACCC TTAAAATTAC CGACTTCAGC TTCAGTGACT TTGAGCTGTC TGATCTGGAA 
ACAGCGCTGT GTACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTGAAGA AGAATTATCG GAAGAACGTT 
GCCTACCACA ATTGGAGGCA TGCCTTTAAC ACGGCACAGT GCATGTTTGC TGCTCTGAAA 
GCCGGCAAGA TCCAGAACAA GTTGACGGAT CTGGAGACCC TTGCGTTGCT CATTGCTGCT 
TTAAGCCATG ACTTGGATCA TCGTGGCGTG AACAACTCAT ACATACAGCG GAGCGAGCAC 
CCGCTGGCTC AGCTGTACTG TCACTCCATC ATGGAACACC ATCATTTTGA CCAGTGCTTG 
ATGATTCTAA ACAGCCCAGG CAACCAGATT CTCAGTGGCC TCTCCATTGA CGAATATAAG 
ACCACACTGA AAATAATCAA GCAAGCAATT TTAGCCACAG ACCTAGCACT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATACGA AAAAATCAAT TCAGTTTTGA AGATCCTCTT 
CAAAAGGAGT TGTTTCTAGC AATGCTGATG ACAGCCTGCG ACCTCTCTGC GATTACTAAA 
CCCTGGCCTA TTCAACAACG GATAGCAGAA CTCGTGGCAG CTGAATTCTT TGATCAAGGA 
GACAGAGAGA GAAAAGAACT TAACATGGAG CCGGCTGATC TAATGAACAG AGAGAAGAAA 
AATAAAATCC CAAGTATGCA AGTTGGGTTC ATAGATGCCA TCTGCTTGCA GCTGTATGAG 
GCCCTGACCC ACGTATCCGA AGACTGTTTG CCCTTGCTGG ATGGCTGCAG GAAGAACAGA 
CAGAAATGGC AGGCCCTCGC GGAGCAGCAG GAGAAGATGC TCCTTAACGG GGAGAGCAGC 
CAGGGCAAGC GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde5a ( XM_006501481.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501544.1 Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31908
Clone ID Related Accession (Same CDS sequence)	XM_006501481.2 , XM_006501481.3
Accession Version	XM_006501481.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCCT TTGGAGACAA AACGAGAGAC ATGGTCAACG CATGGTTTTC AGAGAGAGTT 
CACAACATCC CTGTGTGCAA GGAAGGCATC CGAGCCCATA CCGAGTCCTG CTCTTGCTCT 
CTGCAGCAAA GTCCTCATGC AGATAATACC ACCCCTGGAG CACCAGCCAG AAAAATATCT 
GCCTCTGAAT TTGACCGACC TCTTAGACCC ATTGTTGTCA AGGATTCCGA GGGAACAGTG 
AGCTTTCTCT CTGACTCAGG AAAAAAGGAA CAAATGCCAC TAACCCCCCC AAGATTTGAT 
AGTGATGAAG GGGACCAGTG CTCAAGGCTC TTGGAGCTAG TAAAAGATAT TTCCAGTCAT 
TTGGATGTCA CAGCATTGTG TCACAAAATT TTCTTGCATA TCCATGGACT CATCTCTGCG 
GATCGCTATT CCCTGTTCCT TGTCTGTGAG GACAGCTCTA AAGACAAATT CCTCATCAGC 
CGCCTCTTTG ATGTTGCTGA AGGTTCAACA TTGGAGGAAG CTTCAAATAA CTGTATCCGT 
TTAGAGTGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCTT TTGGCGAGCC CTTGAACATC 
AAAGATGCCT ATGAGGATCC CCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG 
ACACAAAGTA TCCTTTGTAT GCCAATTAAG AACCACAGGG AAGAGGTTGT TGGTGTAGCT 
CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACCTTCA CTGAAAAAGA TGAAAAGGAC 
TTTGCGGCCT ACCTGGCATT CTGTGGTATT GTTCTTCACA ATGCTCAGCT CTATGAAACG 
TCATTGCTGG AGAACAAAAG AAATCAGGTG TTACTTGACC TTGCCAGCTT AATCTTTGAA 
GAGCAGCAGT CACTGGAAGT CATTCTGAAG AAGATAGCGG CCACTATCAT CTCCTTCATG 
CAGGTGCAGA AGTGCACCAT CTTCATTGTG GATGAAGACT GTCCTGACTC TTTCTCTCGT 
GTGTTTCACA TGGAGTGTGA GGAAGTAGGA AAACCCTCTG ATCCTTTAAC GAGGGAGCAA 
GATGCAAACA AAATCAACTA CATGTATGCT CAGTATGTCA AAAACACGAT GGAGCCACTT 
AATATCCCAG ATGTCACCAA GGACAAAAGA TTTCCCTGGA CAAATGAAAA CATGGGACAC 
GTGAACACGC CCTGCATTGG AAGTTTGCTT TGCACCCCTA TAAAAAATGG GAAGAAGAAC 
AAAGTCATAG GCGTCTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGATTAAA 
GCTTTCAACC AAAATGATGA GCAGTTCCTG GAAGCCTTTG TCATCTTCTG TGGCTTGGGG 
ATCCAGAACA CACAGATGTA TGAAGCGGTG GAGAGAGCCA TGGCCAAGCA GATGGTGACA 
TTAGAGGTCC TGTCTTACCA TGCATCAGCG GCGGAGGAAG AAACAAGAGA GCTCCAGGCT 
TTATCGGCTG CTGTGGTACC ATCGGCCCAA ACCCTTAAAA TTACCGACTT CAGCTTCAGT 
GACTTTGAGC TGTCTGATCT GGAAACAGCG CTGTGTACAA TTCGGATGTT CACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGGTGGAT TTTAAGTGTG 
AAGAAGAATT ATCGGAAGAA CGTTGCCTAC CACAATTGGA GGCATGCCTT TAACACGGCA 
CAGTGCATGT TTGCTGCTCT GAAAGCCGGC AAGATCCAGA ACAAGTTGAC GGATCTGGAG 
ACCCTTGCGT TGCTCATTGC TGCTTTAAGC CATGACTTGG ATCATCGTGG CGTGAACAAC 
TCATACATAC AGCGGAGCGA GCACCCGCTG GCTCAGCTGT ACTGTCACTC CATCATGGAA 
CACCATCATT TTGACCAGTG CTTGATGATT CTAAACAGCC CAGGCAACCA GATTCTCAGT 
GGCCTCTCCA TTGACGAATA TAAGACCACA CTGAAAATAA TCAAGCAAGC AATTTTAGCC 
ACAGACCTAG CACTGTACAT TAAGAGACGA GGAGAATTTT TTGAACTTAT ACGAAAAAAT 
CAATTCAGTT TTGAAGATCC TCTTCAAAAG GAGTTGTTTC TAGCAATGCT GATGACAGCC 
TGCGACCTCT CTGCGATTAC TAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTCGTG 
GCAGCTGAAT TCTTTGATCA AGGAGACAGA GAGAGAAAAG AACTTAACAT GGAGCCGGCT 
GATCTAATGA ACAGAGAGAA GAAAAATAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT 
GCCATCTGCT TGCAGCTGTA TGAGGCCCTG ACCCACGTAT CCGAAGACTG TTTGCCCTTG 
CTGGATGGCT GCAGGAAGAA CAGACAGAAA TGGCAGGCCC TCGCGGAGCA GCAGGAGAAG 
ATGCTCCTTA ACGGGGAGAG CAGCCAGGGC AAGCGGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501544.1
CDS	1190..3691
Translation	MLPFGDKTRDMVNAWFSERVHNIPVCKEGIRAHTESCSCSLQQSPHADNTTPGAPARKISASEFDRPLRPIVVKDSEGTVSFLSDSGKKEQMPLTPPRFDSDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCPDSFSRVFHMECEEVGKPSDPLTREQDANKINYMYAQYVKNTMEPLNIPDVTKDKRFPWTNENMGHVNTPCIGSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKIKAFNQNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQALSAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIDEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFSFEDPLQKELFLAMLMTACDLSAITKPWPIQQRIAELVAAEFFDQGDRERKELNMEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCLPLLDGCRKNRQKWQALAEQQEKMLLNGESSQGKRD

Target ORF information:

RefSeq Version	XM_006501481.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501481.2

ORF Insert Sequence:

ATGTTGCCCT TTGGAGACAA AACGAGAGAC ATGGTCAACG CATGGTTTTC AGAGAGAGTT 
CACAACATCC CTGTGTGCAA GGAAGGCATC CGAGCCCATA CCGAGTCCTG CTCTTGCTCT 
CTGCAGCAAA GTCCTCATGC AGATAATACC ACCCCTGGAG CACCAGCCAG AAAAATATCT 
GCCTCTGAAT TTGACCGACC TCTTAGACCC ATTGTTGTCA AGGATTCCGA GGGAACAGTG 
AGCTTTCTCT CTGACTCAGG AAAAAAGGAA CAAATGCCAC TAACCCCCCC AAGATTTGAT 
AGTGATGAAG GGGACCAGTG CTCAAGGCTC TTGGAGCTAG TAAAAGATAT TTCCAGTCAT 
TTGGATGTCA CAGCATTGTG TCACAAAATT TTCTTGCATA TCCATGGACT CATCTCTGCG 
GATCGCTATT CCCTGTTCCT TGTCTGTGAG GACAGCTCTA AAGACAAATT CCTCATCAGC 
CGCCTCTTTG ATGTTGCTGA AGGTTCAACA TTGGAGGAAG CTTCAAATAA CTGTATCCGT 
TTAGAGTGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCTT TTGGCGAGCC CTTGAACATC 
AAAGATGCCT ATGAGGATCC CCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG 
ACACAAAGTA TCCTTTGTAT GCCAATTAAG AACCACAGGG AAGAGGTTGT TGGTGTAGCT 
CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACCTTCA CTGAAAAAGA TGAAAAGGAC 
TTTGCGGCCT ACCTGGCATT CTGTGGTATT GTTCTTCACA ATGCTCAGCT CTATGAAACG 
TCATTGCTGG AGAACAAAAG AAATCAGGTG TTACTTGACC TTGCCAGCTT AATCTTTGAA 
GAGCAGCAGT CACTGGAAGT CATTCTGAAG AAGATAGCGG CCACTATCAT CTCCTTCATG 
CAGGTGCAGA AGTGCACCAT CTTCATTGTG GATGAAGACT GTCCTGACTC TTTCTCTCGT 
GTGTTTCACA TGGAGTGTGA GGAAGTAGGA AAACCCTCTG ATCCTTTAAC GAGGGAGCAA 
GATGCAAACA AAATCAACTA CATGTATGCT CAGTATGTCA AAAACACGAT GGAGCCACTT 
AATATCCCAG ATGTCACCAA GGACAAAAGA TTTCCCTGGA CAAATGAAAA CATGGGACAC 
GTGAACACGC CCTGCATTGG AAGTTTGCTT TGCACCCCTA TAAAAAATGG GAAGAAGAAC 
AAAGTCATAG GCGTCTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGATTAAA 
GCTTTCAACC AAAATGATGA GCAGTTCCTG GAAGCCTTTG TCATCTTCTG TGGCTTGGGG 
ATCCAGAACA CACAGATGTA TGAAGCGGTG GAGAGAGCCA TGGCCAAGCA GATGGTGACA 
TTAGAGGTCC TGTCTTACCA TGCATCAGCG GCGGAGGAAG AAACAAGAGA GCTCCAGGCT 
TTATCGGCTG CTGTGGTACC ATCGGCCCAA ACCCTTAAAA TTACCGACTT CAGCTTCAGT 
GACTTTGAGC TGTCTGATCT GGAAACAGCG CTGTGTACAA TTCGGATGTT CACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGGTGGAT TTTAAGTGTG 
AAGAAGAATT ATCGGAAGAA CGTTGCCTAC CACAATTGGA GGCATGCCTT TAACACGGCA 
CAGTGCATGT TTGCTGCTCT GAAAGCCGGC AAGATCCAGA ACAAGTTGAC GGATCTGGAG 
ACCCTTGCGT TGCTCATTGC TGCTTTAAGC CATGACTTGG ATCATCGTGG CGTGAACAAC 
TCATACATAC AGCGGAGCGA GCACCCGCTG GCTCAGCTGT ACTGTCACTC CATCATGGAA 
CACCATCATT TTGACCAGTG CTTGATGATT CTAAACAGCC CAGGCAACCA GATTCTCAGT 
GGCCTCTCCA TTGACGAATA TAAGACCACA CTGAAAATAA TCAAGCAAGC AATTTTAGCC 
ACAGACCTAG CACTGTACAT TAAGAGACGA GGAGAATTTT TTGAACTTAT ACGAAAAAAT 
CAATTCAGTT TTGAAGATCC TCTTCAAAAG GAGTTGTTTC TAGCAATGCT GATGACAGCC 
TGCGACCTCT CTGCGATTAC TAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTCGTG 
GCAGCTGAAT TCTTTGATCA AGGAGACAGA GAGAGAAAAG AACTTAACAT GGAGCCGGCT 
GATCTAATGA ACAGAGAGAA GAAAAATAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT 
GCCATCTGCT TGCAGCTGTA TGAGGCCCTG ACCCACGTAT CCGAAGACTG TTTGCCCTTG 
CTGGATGGCT GCAGGAAGAA CAGACAGAAA TGGCAGGCCC TCGCGGAGCA GCAGGAGAAG 
ATGCTCCTTA ACGGGGAGAG CAGCCAGGGC AAGCGGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde5a ( XM_006501481.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501544.1 Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), transcript variant X1, mRNA.

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CloneID	OMu31908
Clone ID Related Accession (Same CDS sequence)	XM_006501481.2 , XM_006501481.3
Accession Version	XM_006501481.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501481.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCCT TTGGAGACAA AACGAGAGAC ATGGTCAACG CATGGTTTTC AGAGAGAGTT 
CACAACATCC CTGTGTGCAA GGAAGGCATC CGAGCCCATA CCGAGTCCTG CTCTTGCTCT 
CTGCAGCAAA GTCCTCATGC AGATAATACC ACCCCTGGAG CACCAGCCAG AAAAATATCT 
GCCTCTGAAT TTGACCGACC TCTTAGACCC ATTGTTGTCA AGGATTCCGA GGGAACAGTG 
AGCTTTCTCT CTGACTCAGG AAAAAAGGAA CAAATGCCAC TAACCCCCCC AAGATTTGAT 
AGTGATGAAG GGGACCAGTG CTCAAGGCTC TTGGAGCTAG TAAAAGATAT TTCCAGTCAT 
TTGGATGTCA CAGCATTGTG TCACAAAATT TTCTTGCATA TCCATGGACT CATCTCTGCG 
GATCGCTATT CCCTGTTCCT TGTCTGTGAG GACAGCTCTA AAGACAAATT CCTCATCAGC 
CGCCTCTTTG ATGTTGCTGA AGGTTCAACA TTGGAGGAAG CTTCAAATAA CTGTATCCGT 
TTAGAGTGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCTT TTGGCGAGCC CTTGAACATC 
AAAGATGCCT ATGAGGATCC CCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG 
ACACAAAGTA TCCTTTGTAT GCCAATTAAG AACCACAGGG AAGAGGTTGT TGGTGTAGCT 
CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACCTTCA CTGAAAAAGA TGAAAAGGAC 
TTTGCGGCCT ACCTGGCATT CTGTGGTATT GTTCTTCACA ATGCTCAGCT CTATGAAACG 
TCATTGCTGG AGAACAAAAG AAATCAGGTG TTACTTGACC TTGCCAGCTT AATCTTTGAA 
GAGCAGCAGT CACTGGAAGT CATTCTGAAG AAGATAGCGG CCACTATCAT CTCCTTCATG 
CAGGTGCAGA AGTGCACCAT CTTCATTGTG GATGAAGACT GTCCTGACTC TTTCTCTCGT 
GTGTTTCACA TGGAGTGTGA GGAAGTAGGA AAACCCTCTG ATCCTTTAAC GAGGGAGCAA 
GATGCAAACA AAATCAACTA CATGTATGCT CAGTATGTCA AAAACACGAT GGAGCCACTT 
AATATCCCAG ATGTCACCAA GGACAAAAGA TTTCCCTGGA CAAATGAAAA CATGGGACAC 
GTGAACACGC CCTGCATTGG AAGTTTGCTT TGCACCCCTA TAAAAAATGG GAAGAAGAAC 
AAAGTCATAG GCGTCTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGATTAAA 
GCTTTCAACC AAAATGATGA GCAGTTCCTG GAAGCCTTTG TCATCTTCTG TGGCTTGGGG 
ATCCAGAACA CACAGATGTA TGAAGCGGTG GAGAGAGCCA TGGCCAAGCA GATGGTGACA 
TTAGAGGTCC TGTCTTACCA TGCATCAGCG GCGGAGGAAG AAACAAGAGA GCTCCAGGCT 
TTATCGGCTG CTGTGGTACC ATCGGCCCAA ACCCTTAAAA TTACCGACTT CAGCTTCAGT 
GACTTTGAGC TGTCTGATCT GGAAACAGCG CTGTGTACAA TTCGGATGTT CACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGGTGGAT TTTAAGTGTG 
AAGAAGAATT ATCGGAAGAA CGTTGCCTAC CACAATTGGA GGCATGCCTT TAACACGGCA 
CAGTGCATGT TTGCTGCTCT GAAAGCCGGC AAGATCCAGA ACAAGTTGAC GGATCTGGAG 
ACCCTTGCGT TGCTCATTGC TGCTTTAAGC CATGACTTGG ATCATCGTGG CGTGAACAAC 
TCATACATAC AGCGGAGCGA GCACCCGCTG GCTCAGCTGT ACTGTCACTC CATCATGGAA 
CACCATCATT TTGACCAGTG CTTGATGATT CTAAACAGCC CAGGCAACCA GATTCTCAGT 
GGCCTCTCCA TTGACGAATA TAAGACCACA CTGAAAATAA TCAAGCAAGC AATTTTAGCC 
ACAGACCTAG CACTGTACAT TAAGAGACGA GGAGAATTTT TTGAACTTAT ACGAAAAAAT 
CAATTCAGTT TTGAAGATCC TCTTCAAAAG GAGTTGTTTC TAGCAATGCT GATGACAGCC 
TGCGACCTCT CTGCGATTAC TAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTCGTG 
GCAGCTGAAT TCTTTGATCA AGGAGACAGA GAGAGAAAAG AACTTAACAT GGAGCCGGCT 
GATCTAATGA ACAGAGAGAA GAAAAATAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT 
GCCATCTGCT TGCAGCTGTA TGAGGCCCTG ACCCACGTAT CCGAAGACTG TTTGCCCTTG 
CTGGATGGCT GCAGGAAGAA CAGACAGAAA TGGCAGGCCC TCGCGGAGCA GCAGGAGAAG 
ATGCTCCTTA ACGGGGAGAG CAGCCAGGGC AAGCGGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501544.1
CDS	246..2747
Translation	MLPFGDKTRDMVNAWFSERVHNIPVCKEGIRAHTESCSCSLQQSPHADNTTPGAPARKISASEFDRPLRPIVVKDSEGTVSFLSDSGKKEQMPLTPPRFDSDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCPDSFSRVFHMECEEVGKPSDPLTREQDANKINYMYAQYVKNTMEPLNIPDVTKDKRFPWTNENMGHVNTPCIGSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKIKAFNQNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQALSAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIDEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFSFEDPLQKELFLAMLMTACDLSAITKPWPIQQRIAELVAAEFFDQGDRERKELNMEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCLPLLDGCRKNRQKWQALAEQQEKMLLNGESSQGKRD

Target ORF information:

RefSeq Version	XM_006501481.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501481.3

ORF Insert Sequence:

ATGTTGCCCT TTGGAGACAA AACGAGAGAC ATGGTCAACG CATGGTTTTC AGAGAGAGTT 
CACAACATCC CTGTGTGCAA GGAAGGCATC CGAGCCCATA CCGAGTCCTG CTCTTGCTCT 
CTGCAGCAAA GTCCTCATGC AGATAATACC ACCCCTGGAG CACCAGCCAG AAAAATATCT 
GCCTCTGAAT TTGACCGACC TCTTAGACCC ATTGTTGTCA AGGATTCCGA GGGAACAGTG 
AGCTTTCTCT CTGACTCAGG AAAAAAGGAA CAAATGCCAC TAACCCCCCC AAGATTTGAT 
AGTGATGAAG GGGACCAGTG CTCAAGGCTC TTGGAGCTAG TAAAAGATAT TTCCAGTCAT 
TTGGATGTCA CAGCATTGTG TCACAAAATT TTCTTGCATA TCCATGGACT CATCTCTGCG 
GATCGCTATT CCCTGTTCCT TGTCTGTGAG GACAGCTCTA AAGACAAATT CCTCATCAGC 
CGCCTCTTTG ATGTTGCTGA AGGTTCAACA TTGGAGGAAG CTTCAAATAA CTGTATCCGT 
TTAGAGTGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCTT TTGGCGAGCC CTTGAACATC 
AAAGATGCCT ATGAGGATCC CCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG 
ACACAAAGTA TCCTTTGTAT GCCAATTAAG AACCACAGGG AAGAGGTTGT TGGTGTAGCT 
CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACCTTCA CTGAAAAAGA TGAAAAGGAC 
TTTGCGGCCT ACCTGGCATT CTGTGGTATT GTTCTTCACA ATGCTCAGCT CTATGAAACG 
TCATTGCTGG AGAACAAAAG AAATCAGGTG TTACTTGACC TTGCCAGCTT AATCTTTGAA 
GAGCAGCAGT CACTGGAAGT CATTCTGAAG AAGATAGCGG CCACTATCAT CTCCTTCATG 
CAGGTGCAGA AGTGCACCAT CTTCATTGTG GATGAAGACT GTCCTGACTC TTTCTCTCGT 
GTGTTTCACA TGGAGTGTGA GGAAGTAGGA AAACCCTCTG ATCCTTTAAC GAGGGAGCAA 
GATGCAAACA AAATCAACTA CATGTATGCT CAGTATGTCA AAAACACGAT GGAGCCACTT 
AATATCCCAG ATGTCACCAA GGACAAAAGA TTTCCCTGGA CAAATGAAAA CATGGGACAC 
GTGAACACGC CCTGCATTGG AAGTTTGCTT TGCACCCCTA TAAAAAATGG GAAGAAGAAC 
AAAGTCATAG GCGTCTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGATTAAA 
GCTTTCAACC AAAATGATGA GCAGTTCCTG GAAGCCTTTG TCATCTTCTG TGGCTTGGGG 
ATCCAGAACA CACAGATGTA TGAAGCGGTG GAGAGAGCCA TGGCCAAGCA GATGGTGACA 
TTAGAGGTCC TGTCTTACCA TGCATCAGCG GCGGAGGAAG AAACAAGAGA GCTCCAGGCT 
TTATCGGCTG CTGTGGTACC ATCGGCCCAA ACCCTTAAAA TTACCGACTT CAGCTTCAGT 
GACTTTGAGC TGTCTGATCT GGAAACAGCG CTGTGTACAA TTCGGATGTT CACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGGTGGAT TTTAAGTGTG 
AAGAAGAATT ATCGGAAGAA CGTTGCCTAC CACAATTGGA GGCATGCCTT TAACACGGCA 
CAGTGCATGT TTGCTGCTCT GAAAGCCGGC AAGATCCAGA ACAAGTTGAC GGATCTGGAG 
ACCCTTGCGT TGCTCATTGC TGCTTTAAGC CATGACTTGG ATCATCGTGG CGTGAACAAC 
TCATACATAC AGCGGAGCGA GCACCCGCTG GCTCAGCTGT ACTGTCACTC CATCATGGAA 
CACCATCATT TTGACCAGTG CTTGATGATT CTAAACAGCC CAGGCAACCA GATTCTCAGT 
GGCCTCTCCA TTGACGAATA TAAGACCACA CTGAAAATAA TCAAGCAAGC AATTTTAGCC 
ACAGACCTAG CACTGTACAT TAAGAGACGA GGAGAATTTT TTGAACTTAT ACGAAAAAAT 
CAATTCAGTT TTGAAGATCC TCTTCAAAAG GAGTTGTTTC TAGCAATGCT GATGACAGCC 
TGCGACCTCT CTGCGATTAC TAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTCGTG 
GCAGCTGAAT TCTTTGATCA AGGAGACAGA GAGAGAAAAG AACTTAACAT GGAGCCGGCT 
GATCTAATGA ACAGAGAGAA GAAAAATAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT 
GCCATCTGCT TGCAGCTGTA TGAGGCCCTG ACCCACGTAT CCGAAGACTG TTTGCCCTTG 
CTGGATGGCT GCAGGAAGAA CAGACAGAAA TGGCAGGCCC TCGCGGAGCA GCAGGAGAAG 
ATGCTCCTTA ACGGGGAGAG CAGCCAGGGC AAGCGGGACT GA