PDE5A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE5A
Entrez Gene ID	281972
Full Name	phosphodiesterase 5A
General protein information	Preferred Names phosphodiesterase 5A Names cGMP-specific 3',5'-cyclic phosphodiesterase CGB-PDE cGMP-binding cGMP-specific phosphodiesterase phosphodiesterase 5A, cGMP-specific
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174417.2	NP_776842.1	cGMP-specific 3',5'-cyclic phosphodiesterase
XM_024993001.1	XP_024848769.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
XM_024993002.1	XP_024848770.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pde5ab	NP_001116732.1
Drosophila melanogaster (fruit fly)	Pde11	NP_001188837.1
Pan troglodytes (chimpanzee)	PDE5A	XP_001150967.2
Macaca mulatta (Rhesus monkey)	PDE5A	XP_001099652.1
Canis lupus familiaris (dog)	PDE5A	NP_001003188.1
Bos taurus (cattle)	PDE5A	NP_776842.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008646	XP_314743.3
Rattus norvegicus (Norway rat)	Pde5a	NP_598268.1
Xenopus tropicalis (tropical clawed frog)	pde5a	NP_001072812.1
Homo sapiens (human)	PDE5A	NP_001074.2
Mus musculus (house mouse)	Pde5a	NP_700471.2
Gallus gallus (chicken)	PDE5A	XP_420631.3

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Ectopic expression of bovine type 5 phosphodiesterase confers a renal phenotype in Drosophila.
Broderick KE, Kean L, Dow JA, Pyne NJ, Davies SA
The Journal of biological chemistry279(9)8159-68(2004 Feb)

Binding of cGMP to both allosteric sites of cGMP-binding cGMP-specific phosphodiesterase (PDE5) is required for its phosphorylation.
Turko IV, Francis SH, Corbin JD
The Biochemical journal329 ( Pt 3)505-10(1998 Feb)

Identification of key amino acids in a conserved cGMP-binding site of cGMP-binding phosphodiesterases. A putative NKXnD motif for cGMP binding.
Turko IV, Haik TL, McAllister-Lucas LM, Burns F, Francis SH, Corbin JD
The Journal of biological chemistry271(36)22240-4(1996 Sep)

An essential aspartic acid at each of two allosteric cGMP-binding sites of a cGMP-specific phosphodiesterase.
McAllister-Lucas LM, Haik TL, Colbran JL, Sonnenburg WK, Seger D, Turko IV, Beavo JA, Francis SH, Corbin JD
The Journal of biological chemistry270(51)30671-9(1995 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PDE5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41342	NM_174417.2 Latest version!	Bos taurus phosphodiesterase 5A (PDE5A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa235118	XM_024993001.1 Latest version!	Bos taurus phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa235119	XM_024993002.1 Latest version!	Bos taurus phosphodiesterase 5A (PDE5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE5A ( NM_174417.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776842.1 Bos taurus phosphodiesterase 5A (PDE5A), mRNA.

Price & Availability↑

CloneID	OBa41342
Clone ID Related Accession (Same CDS sequence)	NM_174417.2
Accession Version	NM_174417.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571414400000
Organism	Bos taurus(cattle)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L16545.1. On or before Mar 29, 2005 this sequence version replaced XM_602967.1, NM_174417.1. ##Evidence-Data-START## Transcript exon combination :: L16545.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145424 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAGGG CCGGCCCCGG CTCCGCGCGG CCGCAACAGC AATGGGACCA GGACTCGGTC 
GAAGCGTGGC TGGACGATCA CTGGGACTTT ACCTTCTCTT ACTTTGTTAG GAAAGGCACC 
AGAGAAATGG TCAACGCATG GTTTGCTGAG AGAGTTCACA CCATTCCTGT GTGCAAGGAA 
GGAATCAAGG GCCACACGGA ATCCTGCTCT TGCCCCTTGC AGCCAAGTCC CCGTGCAGAG 
AGCAGTGTCC CTGGAACACC AACCAGGAAG ATCTCTGCCT CTGAATTCGA TCGGCCGCTT 
AGACCCATCG TTATCAAGGA TTCTGAGGGA ACTGTGAGCT TCCTCTCTGA CTCAGACAAG 
AAGGAACAGA TGCCTCTAAC CTCCCCACGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCACTTGG ATGTCACAGC CTTATGTCAC 
AAAATTTTCT TGCACATCCA TGGACTCATC TCCGCCGACC GCTACTCCTT ATTCCTCGTC 
TGTGAGGACA GCTCCAACGA CAAGTTTCTT ATCAGCCGCC TCTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAACAACTGC ATCCGCTTAG AGTGGAACAA AGGCATCGTG 
GGACACGTGG CCGCTTTTGG CGAGCCCTTG AACATCAAAG ACGCCTATGA GGATCCTCGA 
TTCAATGCAG AAGTTGACCA AATTACAGGC TACAAGACAC AAAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGACGAA AAGGACTTTG CTGCTTACTT GGCATTTTGT 
GGAATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGTGTGT TTCACATGGA GTGTGAGGAA 
TTAGAAAAAT CGTCAGATAC TTTAACACGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACCATGGAA CCACTTAATA TCCCAGACGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACATG GGAAATATAA ACCAGCAGTG CATTAGAAGT 
TTGCTTTGTA CACCTATAAA AAATGGAAAG AAGAACAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAAAC CACTGGCAAA GTTAAGGCTT TCAACCGCAA CGATGAACAG 
TTTCTGGAAG CTTTCGTCAT CTTTTGTGGC TTGGGGATCC AGAACACACA GATGTACGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAAATG GTCACGTTAG AGGTTCTGTC TTATCATGCT 
TCAGCTGCAG AGGAAGAAAC CAGAGAGCTG CAGTCCTTAG CGGCTGCTGT GGTACCATCT 
GCCCAGACCC TTAAAATCAC TGACTTCAGC TTCAGCGACT TTGAGCTGTC TGACCTGGAA 
ACAGCACTGT GCACAATCCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TCCTTTGCAA GTGGATTTTA AGTGTGAAGA AGAACTATCG GAAGAACGTC 
GCCTATCATA ATTGGAGACA TGCCTTTAAT ACAGCTCAGT GCATGTTTGC GGCACTAAAA 
GCAGGCAAAA TTCAGAAGAG GCTGACGGAC CTGGAGATAC TTGCACTGCT GATTGCTGCC 
TTAAGCCATG ATCTGGATCA CCGTGGTGTC AATAACTCAT ACATACAGCG AAGTGAACAC 
CCACTTGCTC AGCTCTACTG CCATTCAATC ATGGAGCATC ATCATTTTGA TCAGTGCCTG 
ATGATCCTTA ATAGTCCTGG CAATCAGATT CTCAGTGGCC TCTCCATTGA AGAGTATAAG 
ACCACCCTGA AGATCATCAA GCAAGCTATT TTAGCCACAG ACCTAGCACT GTACATAAAG 
AGACGAGGAG AATTTTTTGA ACTTATAATG AAAAATCAAT TCAATTTGGA AGATCCTCAT 
CAAAAGGAGT TGTTTTTAGC GATGCTGATG ACAGCTTGTG ATCTTTCTGC AATTACAAAA 
CCCTGGCCTA TTCAACAACG GATAGCAGAA CTTGTTGCCA CTGAATTTTT TGACCAAGGA 
GATAGAGAGA GGAAAGAACT CAACATAGAG CCCGCTGATC TAATGAACCG GGAGAAGAAA 
AACAAAATCC CAAGTATGCA AGTTGGATTC ATAGATGCCA TCTGCTTGCA ACTGTATGAG 
GCCTTGACCC ATGTGTCGGA GGACTGTTTC CCTTTGCTGG ACGGCTGCAG AAAGAACAGG 
CAGAAATGGC AGGCTCTTGC AGAACAGCAG GAGAAGACAC TGATCAATGG TGAAAGCAGC 
CAGACCAAGC GGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776842.1
CDS	99..2696
Translation	MERAGPGSARPQQQWDQDSVEAWLDDHWDFTFSYFVRKGTREMVNAWFAERVHTIPVCKEGIKGHTESCSCPLQPSPRAESSVPGTPTRKISASEFDRPLRPIVIKDSEGTVSFLSDSDKKEQMPLTSPRFDNDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTRERDANRINYMYAQYVKNTMEPLNIPDVSKDKRFPWTNENMGNINQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEETTGKVKAFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCKWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQKRLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIMKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKTLINGESSQTKRN

Target ORF information:

RefSeq Version	NM_174417.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 5A (PDE5A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174417.2

ORF Insert Sequence:

ATGGAGAGGG CCGGCCCCGG CTCCGCGCGG CCGCAACAGC AATGGGACCA GGACTCGGTC 
GAAGCGTGGC TGGACGATCA CTGGGACTTT ACCTTCTCTT ACTTTGTTAG GAAAGGCACC 
AGAGAAATGG TCAACGCATG GTTTGCTGAG AGAGTTCACA CCATTCCTGT GTGCAAGGAA 
GGAATCAAGG GCCACACGGA ATCCTGCTCT TGCCCCTTGC AGCCAAGTCC CCGTGCAGAG 
AGCAGTGTCC CTGGAACACC AACCAGGAAG ATCTCTGCCT CTGAATTCGA TCGGCCGCTT 
AGACCCATCG TTATCAAGGA TTCTGAGGGA ACTGTGAGCT TCCTCTCTGA CTCAGACAAG 
AAGGAACAGA TGCCTCTAAC CTCCCCACGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCACTTGG ATGTCACAGC CTTATGTCAC 
AAAATTTTCT TGCACATCCA TGGACTCATC TCCGCCGACC GCTACTCCTT ATTCCTCGTC 
TGTGAGGACA GCTCCAACGA CAAGTTTCTT ATCAGCCGCC TCTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAACAACTGC ATCCGCTTAG AGTGGAACAA AGGCATCGTG 
GGACACGTGG CCGCTTTTGG CGAGCCCTTG AACATCAAAG ACGCCTATGA GGATCCTCGA 
TTCAATGCAG AAGTTGACCA AATTACAGGC TACAAGACAC AAAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGACGAA AAGGACTTTG CTGCTTACTT GGCATTTTGT 
GGAATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGTGTGT TTCACATGGA GTGTGAGGAA 
TTAGAAAAAT CGTCAGATAC TTTAACACGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACCATGGAA CCACTTAATA TCCCAGACGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACATG GGAAATATAA ACCAGCAGTG CATTAGAAGT 
TTGCTTTGTA CACCTATAAA AAATGGAAAG AAGAACAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAAAC CACTGGCAAA GTTAAGGCTT TCAACCGCAA CGATGAACAG 
TTTCTGGAAG CTTTCGTCAT CTTTTGTGGC TTGGGGATCC AGAACACACA GATGTACGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAAATG GTCACGTTAG AGGTTCTGTC TTATCATGCT 
TCAGCTGCAG AGGAAGAAAC CAGAGAGCTG CAGTCCTTAG CGGCTGCTGT GGTACCATCT 
GCCCAGACCC TTAAAATCAC TGACTTCAGC TTCAGCGACT TTGAGCTGTC TGACCTGGAA 
ACAGCACTGT GCACAATCCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TCCTTTGCAA GTGGATTTTA AGTGTGAAGA AGAACTATCG GAAGAACGTC 
GCCTATCATA ATTGGAGACA TGCCTTTAAT ACAGCTCAGT GCATGTTTGC GGCACTAAAA 
GCAGGCAAAA TTCAGAAGAG GCTGACGGAC CTGGAGATAC TTGCACTGCT GATTGCTGCC 
TTAAGCCATG ATCTGGATCA CCGTGGTGTC AATAACTCAT ACATACAGCG AAGTGAACAC 
CCACTTGCTC AGCTCTACTG CCATTCAATC ATGGAGCATC ATCATTTTGA TCAGTGCCTG 
ATGATCCTTA ATAGTCCTGG CAATCAGATT CTCAGTGGCC TCTCCATTGA AGAGTATAAG 
ACCACCCTGA AGATCATCAA GCAAGCTATT TTAGCCACAG ACCTAGCACT GTACATAAAG 
AGACGAGGAG AATTTTTTGA ACTTATAATG AAAAATCAAT TCAATTTGGA AGATCCTCAT 
CAAAAGGAGT TGTTTTTAGC GATGCTGATG ACAGCTTGTG ATCTTTCTGC AATTACAAAA 
CCCTGGCCTA TTCAACAACG GATAGCAGAA CTTGTTGCCA CTGAATTTTT TGACCAAGGA 
GATAGAGAGA GGAAAGAACT CAACATAGAG CCCGCTGATC TAATGAACCG GGAGAAGAAA 
AACAAAATCC CAAGTATGCA AGTTGGATTC ATAGATGCCA TCTGCTTGCA ACTGTATGAG 
GCCTTGACCC ATGTGTCGGA GGACTGTTTC CCTTTGCTGG ACGGCTGCAG AAAGAACAGG 
CAGAAATGGC AGGCTCTTGC AGAACAGCAG GAGAAGACAC TGATCAATGG TGAAAGCAGC 
CAGACCAAGC GGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE5A ( XM_024993001.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848769.1 Bos taurus phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa235118
Clone ID Related Accession (Same CDS sequence)	XM_024993001.1
Accession Version	XM_024993001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCTT TTGGAGACAA AACAAGAGAA ATGGTCAACG CATGGTTTGC TGAGAGAGTT 
CACACCATTC CTGTGTGCAA GGAAGGAATC AAGGGCCACA CGGAATCCTG CTCTTGCCCC 
TTGCAGCCAA GTCCCCGTGC AGAGAGCAGT GTCCCTGGAA CACCAACCAG GAAGATCTCT 
GCCTCTGAAT TCGATCGGCC GCTTAGACCC ATCGTTATCA AGGATTCTGA GGGAACTGTG 
AGCTTCCTCT CTGACTCAGA CAAGAAGGAA CAGATGCCTC TAACCTCCCC ACGGTTTGAT 
AATGATGAAG GGGACCAGTG CTCGAGACTC TTGGAATTAG TGAAAGATAT TTCTAGTCAC 
TTGGATGTCA CAGCCTTATG TCACAAAATT TTCTTGCACA TCCATGGACT CATCTCCGCC 
GACCGCTACT CCTTATTCCT CGTCTGTGAG GACAGCTCCA ACGACAAGTT TCTTATCAGC 
CGCCTCTTTG ATGTTGCAGA AGGTTCAACA CTGGAAGAAG CTTCAAACAA CTGCATCCGC 
TTAGAGTGGA ACAAAGGCAT CGTGGGACAC GTGGCCGCTT TTGGCGAGCC CTTGAACATC 
AAAGACGCCT ATGAGGATCC TCGATTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG 
ACACAAAGTA TTCTTTGTAT GCCAATTAAG AATCATAGGG AAGAGGTTGT TGGTGTAGCC 
CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACATTCA CTGAAAAAGA CGAAAAGGAC 
TTTGCTGCTT ACTTGGCATT TTGTGGAATT GTTCTTCATA ATGCTCAACT CTATGAGACT 
TCACTGCTGG AGAACAAGAG AAATCAGGTG CTGCTTGACC TTGCTAGCTT AATTTTTGAA 
GAACAACAAT CATTAGAAGT AATTCTAAAG AAAATAGCTG CCACTATTAT CTCTTTCATG 
CAGGTGCAGA AATGCACCAT TTTCATAGTG GATGAAGATT GCTCCGATTC TTTTTCTAGT 
GTGTTTCACA TGGAGTGTGA GGAATTAGAA AAATCGTCAG ATACTTTAAC ACGGGAACGT 
GATGCAAACA GAATCAATTA CATGTATGCT CAGTATGTCA AAAATACCAT GGAACCACTT 
AATATCCCAG ACGTCAGTAA GGACAAAAGA TTTCCCTGGA CAAATGAAAA CATGGGAAAT 
ATAAACCAGC AGTGCATTAG AAGTTTGCTT TGTACACCTA TAAAAAATGG AAAGAAGAAC 
AAAGTGATAG GGGTTTGCCA ACTTGTTAAT AAGATGGAGG AAACCACTGG CAAAGTTAAG 
GCTTTCAACC GCAACGATGA ACAGTTTCTG GAAGCTTTCG TCATCTTTTG TGGCTTGGGG 
ATCCAGAACA CACAGATGTA CGAAGCAGTG GAGAGAGCCA TGGCCAAGCA AATGGTCACG 
TTAGAGGTTC TGTCTTATCA TGCTTCAGCT GCAGAGGAAG AAACCAGAGA GCTGCAGTCC 
TTAGCGGCTG CTGTGGTACC ATCTGCCCAG ACCCTTAAAA TCACTGACTT CAGCTTCAGC 
GACTTTGAGC TGTCTGACCT GGAAACAGCA CTGTGCACAA TCCGGATGTT CACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTCCTTT GCAAGTGGAT TTTAAGTGTG 
AAGAAGAACT ATCGGAAGAA CGTCGCCTAT CATAATTGGA GACATGCCTT TAATACAGCT 
CAGTGCATGT TTGCGGCACT AAAAGCAGGC AAAATTCAGA AGAGGCTGAC GGACCTGGAG 
ATACTTGCAC TGCTGATTGC TGCCTTAAGC CATGATCTGG ATCACCGTGG TGTCAATAAC 
TCATACATAC AGCGAAGTGA ACACCCACTT GCTCAGCTCT ACTGCCATTC AATCATGGAG 
CATCATCATT TTGATCAGTG CCTGATGATC CTTAATAGTC CTGGCAATCA GATTCTCAGT 
GGCCTCTCCA TTGAAGAGTA TAAGACCACC CTGAAGATCA TCAAGCAAGC TATTTTAGCC 
ACAGACCTAG CACTGTACAT AAAGAGACGA GGAGAATTTT TTGAACTTAT AATGAAAAAT 
CAATTCAATT TGGAAGATCC TCATCAAAAG GAGTTGTTTT TAGCGATGCT GATGACAGCT 
TGTGATCTTT CTGCAATTAC AAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTTGTT 
GCCACTGAAT TTTTTGACCA AGGAGATAGA GAGAGGAAAG AACTCAACAT AGAGCCCGCT 
GATCTAATGA ACCGGGAGAA GAAAAACAAA ATCCCAAGTA TGCAAGTTGG ATTCATAGAT 
GCCATCTGCT TGCAACTGTA TGAGGCCTTG ACCCATGTGT CGGAGGACTG TTTCCCTTTG 
CTGGACGGCT GCAGAAAGAA CAGGCAGAAA TGGCAGGCTC TTGCAGAACA GCAGGAGAAG 
ACACTGATCA ATGGTGAAAG CAGCCAGACC AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848769.1
CDS	162..2663
Translation	MLPFGDKTREMVNAWFAERVHTIPVCKEGIKGHTESCSCPLQPSPRAESSVPGTPTRKISASEFDRPLRPIVIKDSEGTVSFLSDSDKKEQMPLTSPRFDNDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTRERDANRINYMYAQYVKNTMEPLNIPDVSKDKRFPWTNENMGNINQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEETTGKVKAFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCKWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQKRLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIMKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKTLINGESSQTKRN

Target ORF information:

RefSeq Version	XM_024993001.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993001.1

ORF Insert Sequence:

ATGTTGCCTT TTGGAGACAA AACAAGAGAA ATGGTCAACG CATGGTTTGC TGAGAGAGTT 
CACACCATTC CTGTGTGCAA GGAAGGAATC AAGGGCCACA CGGAATCCTG CTCTTGCCCC 
TTGCAGCCAA GTCCCCGTGC AGAGAGCAGT GTCCCTGGAA CACCAACCAG GAAGATCTCT 
GCCTCTGAAT TCGATCGGCC GCTTAGACCC ATCGTTATCA AGGATTCTGA GGGAACTGTG 
AGCTTCCTCT CTGACTCAGA CAAGAAGGAA CAGATGCCTC TAACCTCCCC ACGGTTTGAT 
AATGATGAAG GGGACCAGTG CTCGAGACTC TTGGAATTAG TGAAAGATAT TTCTAGTCAC 
TTGGATGTCA CAGCCTTATG TCACAAAATT TTCTTGCACA TCCATGGACT CATCTCCGCC 
GACCGCTACT CCTTATTCCT CGTCTGTGAG GACAGCTCCA ACGACAAGTT TCTTATCAGC 
CGCCTCTTTG ATGTTGCAGA AGGTTCAACA CTGGAAGAAG CTTCAAACAA CTGCATCCGC 
TTAGAGTGGA ACAAAGGCAT CGTGGGACAC GTGGCCGCTT TTGGCGAGCC CTTGAACATC 
AAAGACGCCT ATGAGGATCC TCGATTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG 
ACACAAAGTA TTCTTTGTAT GCCAATTAAG AATCATAGGG AAGAGGTTGT TGGTGTAGCC 
CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACATTCA CTGAAAAAGA CGAAAAGGAC 
TTTGCTGCTT ACTTGGCATT TTGTGGAATT GTTCTTCATA ATGCTCAACT CTATGAGACT 
TCACTGCTGG AGAACAAGAG AAATCAGGTG CTGCTTGACC TTGCTAGCTT AATTTTTGAA 
GAACAACAAT CATTAGAAGT AATTCTAAAG AAAATAGCTG CCACTATTAT CTCTTTCATG 
CAGGTGCAGA AATGCACCAT TTTCATAGTG GATGAAGATT GCTCCGATTC TTTTTCTAGT 
GTGTTTCACA TGGAGTGTGA GGAATTAGAA AAATCGTCAG ATACTTTAAC ACGGGAACGT 
GATGCAAACA GAATCAATTA CATGTATGCT CAGTATGTCA AAAATACCAT GGAACCACTT 
AATATCCCAG ACGTCAGTAA GGACAAAAGA TTTCCCTGGA CAAATGAAAA CATGGGAAAT 
ATAAACCAGC AGTGCATTAG AAGTTTGCTT TGTACACCTA TAAAAAATGG AAAGAAGAAC 
AAAGTGATAG GGGTTTGCCA ACTTGTTAAT AAGATGGAGG AAACCACTGG CAAAGTTAAG 
GCTTTCAACC GCAACGATGA ACAGTTTCTG GAAGCTTTCG TCATCTTTTG TGGCTTGGGG 
ATCCAGAACA CACAGATGTA CGAAGCAGTG GAGAGAGCCA TGGCCAAGCA AATGGTCACG 
TTAGAGGTTC TGTCTTATCA TGCTTCAGCT GCAGAGGAAG AAACCAGAGA GCTGCAGTCC 
TTAGCGGCTG CTGTGGTACC ATCTGCCCAG ACCCTTAAAA TCACTGACTT CAGCTTCAGC 
GACTTTGAGC TGTCTGACCT GGAAACAGCA CTGTGCACAA TCCGGATGTT CACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTCCTTT GCAAGTGGAT TTTAAGTGTG 
AAGAAGAACT ATCGGAAGAA CGTCGCCTAT CATAATTGGA GACATGCCTT TAATACAGCT 
CAGTGCATGT TTGCGGCACT AAAAGCAGGC AAAATTCAGA AGAGGCTGAC GGACCTGGAG 
ATACTTGCAC TGCTGATTGC TGCCTTAAGC CATGATCTGG ATCACCGTGG TGTCAATAAC 
TCATACATAC AGCGAAGTGA ACACCCACTT GCTCAGCTCT ACTGCCATTC AATCATGGAG 
CATCATCATT TTGATCAGTG CCTGATGATC CTTAATAGTC CTGGCAATCA GATTCTCAGT 
GGCCTCTCCA TTGAAGAGTA TAAGACCACC CTGAAGATCA TCAAGCAAGC TATTTTAGCC 
ACAGACCTAG CACTGTACAT AAAGAGACGA GGAGAATTTT TTGAACTTAT AATGAAAAAT 
CAATTCAATT TGGAAGATCC TCATCAAAAG GAGTTGTTTT TAGCGATGCT GATGACAGCT 
TGTGATCTTT CTGCAATTAC AAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTTGTT 
GCCACTGAAT TTTTTGACCA AGGAGATAGA GAGAGGAAAG AACTCAACAT AGAGCCCGCT 
GATCTAATGA ACCGGGAGAA GAAAAACAAA ATCCCAAGTA TGCAAGTTGG ATTCATAGAT 
GCCATCTGCT TGCAACTGTA TGAGGCCTTG ACCCATGTGT CGGAGGACTG TTTCCCTTTG 
CTGGACGGCT GCAGAAAGAA CAGGCAGAAA TGGCAGGCTC TTGCAGAACA GCAGGAGAAG 
ACACTGATCA ATGGTGAAAG CAGCCAGACC AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE5A ( XM_024993002.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848770.1 Bos taurus phosphodiesterase 5A (PDE5A), transcript variant X2, mRNA.

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CloneID	OBa235119
Clone ID Related Accession (Same CDS sequence)	XM_024993002.1
Accession Version	XM_024993002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAACG CATGGTTTGC TGAGAGAGTT CACACCATTC CTGTGTGCAA GGAAGGAATC 
AAGGGCCACA CGGAATCCTG CTCTTGCCCC TTGCAGCCAA GTCCCCGTGC AGAGAGCAGT 
GTCCCTGGAA CACCAACCAG GAAGATCTCT GCCTCTGAAT TCGATCGGCC GCTTAGACCC 
ATCGTTATCA AGGATTCTGA GGGAACTGTG AGCTTCCTCT CTGACTCAGA CAAGAAGGAA 
CAGATGCCTC TAACCTCCCC ACGGTTTGAT AATGATGAAG GGGACCAGTG CTCGAGACTC 
TTGGAATTAG TGAAAGATAT TTCTAGTCAC TTGGATGTCA CAGCCTTATG TCACAAAATT 
TTCTTGCACA TCCATGGACT CATCTCCGCC GACCGCTACT CCTTATTCCT CGTCTGTGAG 
GACAGCTCCA ACGACAAGTT TCTTATCAGC CGCCTCTTTG ATGTTGCAGA AGGTTCAACA 
CTGGAAGAAG CTTCAAACAA CTGCATCCGC TTAGAGTGGA ACAAAGGCAT CGTGGGACAC 
GTGGCCGCTT TTGGCGAGCC CTTGAACATC AAAGACGCCT ATGAGGATCC TCGATTCAAT 
GCAGAAGTTG ACCAAATTAC AGGCTACAAG ACACAAAGTA TTCTTTGTAT GCCAATTAAG 
AATCATAGGG AAGAGGTTGT TGGTGTAGCC CAGGCCATCA ACAAGAAATC AGGAAATGGT 
GGGACATTCA CTGAAAAAGA CGAAAAGGAC TTTGCTGCTT ACTTGGCATT TTGTGGAATT 
GTTCTTCATA ATGCTCAACT CTATGAGACT TCACTGCTGG AGAACAAGAG AAATCAGGTG 
CTGCTTGACC TTGCTAGCTT AATTTTTGAA GAACAACAAT CATTAGAAGT AATTCTAAAG 
AAAATAGCTG CCACTATTAT CTCTTTCATG CAGGTGCAGA AATGCACCAT TTTCATAGTG 
GATGAAGATT GCTCCGATTC TTTTTCTAGT GTGTTTCACA TGGAGTGTGA GGAATTAGAA 
AAATCGTCAG ATACTTTAAC ACGGGAACGT GATGCAAACA GAATCAATTA CATGTATGCT 
CAGTATGTCA AAAATACCAT GGAACCACTT AATATCCCAG ACGTCAGTAA GGACAAAAGA 
TTTCCCTGGA CAAATGAAAA CATGGGAAAT ATAAACCAGC AGTGCATTAG AAGTTTGCTT 
TGTACACCTA TAAAAAATGG AAAGAAGAAC AAAGTGATAG GGGTTTGCCA ACTTGTTAAT 
AAGATGGAGG AAACCACTGG CAAAGTTAAG GCTTTCAACC GCAACGATGA ACAGTTTCTG 
GAAGCTTTCG TCATCTTTTG TGGCTTGGGG ATCCAGAACA CACAGATGTA CGAAGCAGTG 
GAGAGAGCCA TGGCCAAGCA AATGGTCACG TTAGAGGTTC TGTCTTATCA TGCTTCAGCT 
GCAGAGGAAG AAACCAGAGA GCTGCAGTCC TTAGCGGCTG CTGTGGTACC ATCTGCCCAG 
ACCCTTAAAA TCACTGACTT CAGCTTCAGC GACTTTGAGC TGTCTGACCT GGAAACAGCA 
CTGTGCACAA TCCGGATGTT CACTGACCTC AACCTTGTGC AGAACTTCCA GATGAAACAT 
GAGGTCCTTT GCAAGTGGAT TTTAAGTGTG AAGAAGAACT ATCGGAAGAA CGTCGCCTAT 
CATAATTGGA GACATGCCTT TAATACAGCT CAGTGCATGT TTGCGGCACT AAAAGCAGGC 
AAAATTCAGA AGAGGCTGAC GGACCTGGAG ATACTTGCAC TGCTGATTGC TGCCTTAAGC 
CATGATCTGG ATCACCGTGG TGTCAATAAC TCATACATAC AGCGAAGTGA ACACCCACTT 
GCTCAGCTCT ACTGCCATTC AATCATGGAG CATCATCATT TTGATCAGTG CCTGATGATC 
CTTAATAGTC CTGGCAATCA GATTCTCAGT GGCCTCTCCA TTGAAGAGTA TAAGACCACC 
CTGAAGATCA TCAAGCAAGC TATTTTAGCC ACAGACCTAG CACTGTACAT AAAGAGACGA 
GGAGAATTTT TTGAACTTAT AATGAAAAAT CAATTCAATT TGGAAGATCC TCATCAAAAG 
GAGTTGTTTT TAGCGATGCT GATGACAGCT TGTGATCTTT CTGCAATTAC AAAACCCTGG 
CCTATTCAAC AACGGATAGC AGAACTTGTT GCCACTGAAT TTTTTGACCA AGGAGATAGA 
GAGAGGAAAG AACTCAACAT AGAGCCCGCT GATCTAATGA ACCGGGAGAA GAAAAACAAA 
ATCCCAAGTA TGCAAGTTGG ATTCATAGAT GCCATCTGCT TGCAACTGTA TGAGGCCTTG 
ACCCATGTGT CGGAGGACTG TTTCCCTTTG CTGGACGGCT GCAGAAAGAA CAGGCAGAAA 
TGGCAGGCTC TTGCAGAACA GCAGGAGAAG ACACTGATCA ATGGTGAAAG CAGCCAGACC 
AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848770.1
CDS	275..2746
Translation	MVNAWFAERVHTIPVCKEGIKGHTESCSCPLQPSPRAESSVPGTPTRKISASEFDRPLRPIVIKDSEGTVSFLSDSDKKEQMPLTSPRFDNDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTRERDANRINYMYAQYVKNTMEPLNIPDVSKDKRFPWTNENMGNINQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEETTGKVKAFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCKWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQKRLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIMKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKTLINGESSQTKRN

Target ORF information:

RefSeq Version	XM_024993002.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 5A (PDE5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993002.1

ORF Insert Sequence:

ATGGTCAACG CATGGTTTGC TGAGAGAGTT CACACCATTC CTGTGTGCAA GGAAGGAATC 
AAGGGCCACA CGGAATCCTG CTCTTGCCCC TTGCAGCCAA GTCCCCGTGC AGAGAGCAGT 
GTCCCTGGAA CACCAACCAG GAAGATCTCT GCCTCTGAAT TCGATCGGCC GCTTAGACCC 
ATCGTTATCA AGGATTCTGA GGGAACTGTG AGCTTCCTCT CTGACTCAGA CAAGAAGGAA 
CAGATGCCTC TAACCTCCCC ACGGTTTGAT AATGATGAAG GGGACCAGTG CTCGAGACTC 
TTGGAATTAG TGAAAGATAT TTCTAGTCAC TTGGATGTCA CAGCCTTATG TCACAAAATT 
TTCTTGCACA TCCATGGACT CATCTCCGCC GACCGCTACT CCTTATTCCT CGTCTGTGAG 
GACAGCTCCA ACGACAAGTT TCTTATCAGC CGCCTCTTTG ATGTTGCAGA AGGTTCAACA 
CTGGAAGAAG CTTCAAACAA CTGCATCCGC TTAGAGTGGA ACAAAGGCAT CGTGGGACAC 
GTGGCCGCTT TTGGCGAGCC CTTGAACATC AAAGACGCCT ATGAGGATCC TCGATTCAAT 
GCAGAAGTTG ACCAAATTAC AGGCTACAAG ACACAAAGTA TTCTTTGTAT GCCAATTAAG 
AATCATAGGG AAGAGGTTGT TGGTGTAGCC CAGGCCATCA ACAAGAAATC AGGAAATGGT 
GGGACATTCA CTGAAAAAGA CGAAAAGGAC TTTGCTGCTT ACTTGGCATT TTGTGGAATT 
GTTCTTCATA ATGCTCAACT CTATGAGACT TCACTGCTGG AGAACAAGAG AAATCAGGTG 
CTGCTTGACC TTGCTAGCTT AATTTTTGAA GAACAACAAT CATTAGAAGT AATTCTAAAG 
AAAATAGCTG CCACTATTAT CTCTTTCATG CAGGTGCAGA AATGCACCAT TTTCATAGTG 
GATGAAGATT GCTCCGATTC TTTTTCTAGT GTGTTTCACA TGGAGTGTGA GGAATTAGAA 
AAATCGTCAG ATACTTTAAC ACGGGAACGT GATGCAAACA GAATCAATTA CATGTATGCT 
CAGTATGTCA AAAATACCAT GGAACCACTT AATATCCCAG ACGTCAGTAA GGACAAAAGA 
TTTCCCTGGA CAAATGAAAA CATGGGAAAT ATAAACCAGC AGTGCATTAG AAGTTTGCTT 
TGTACACCTA TAAAAAATGG AAAGAAGAAC AAAGTGATAG GGGTTTGCCA ACTTGTTAAT 
AAGATGGAGG AAACCACTGG CAAAGTTAAG GCTTTCAACC GCAACGATGA ACAGTTTCTG 
GAAGCTTTCG TCATCTTTTG TGGCTTGGGG ATCCAGAACA CACAGATGTA CGAAGCAGTG 
GAGAGAGCCA TGGCCAAGCA AATGGTCACG TTAGAGGTTC TGTCTTATCA TGCTTCAGCT 
GCAGAGGAAG AAACCAGAGA GCTGCAGTCC TTAGCGGCTG CTGTGGTACC ATCTGCCCAG 
ACCCTTAAAA TCACTGACTT CAGCTTCAGC GACTTTGAGC TGTCTGACCT GGAAACAGCA 
CTGTGCACAA TCCGGATGTT CACTGACCTC AACCTTGTGC AGAACTTCCA GATGAAACAT 
GAGGTCCTTT GCAAGTGGAT TTTAAGTGTG AAGAAGAACT ATCGGAAGAA CGTCGCCTAT 
CATAATTGGA GACATGCCTT TAATACAGCT CAGTGCATGT TTGCGGCACT AAAAGCAGGC 
AAAATTCAGA AGAGGCTGAC GGACCTGGAG ATACTTGCAC TGCTGATTGC TGCCTTAAGC 
CATGATCTGG ATCACCGTGG TGTCAATAAC TCATACATAC AGCGAAGTGA ACACCCACTT 
GCTCAGCTCT ACTGCCATTC AATCATGGAG CATCATCATT TTGATCAGTG CCTGATGATC 
CTTAATAGTC CTGGCAATCA GATTCTCAGT GGCCTCTCCA TTGAAGAGTA TAAGACCACC 
CTGAAGATCA TCAAGCAAGC TATTTTAGCC ACAGACCTAG CACTGTACAT AAAGAGACGA 
GGAGAATTTT TTGAACTTAT AATGAAAAAT CAATTCAATT TGGAAGATCC TCATCAAAAG 
GAGTTGTTTT TAGCGATGCT GATGACAGCT TGTGATCTTT CTGCAATTAC AAAACCCTGG 
CCTATTCAAC AACGGATAGC AGAACTTGTT GCCACTGAAT TTTTTGACCA AGGAGATAGA 
GAGAGGAAAG AACTCAACAT AGAGCCCGCT GATCTAATGA ACCGGGAGAA GAAAAACAAA 
ATCCCAAGTA TGCAAGTTGG ATTCATAGAT GCCATCTGCT TGCAACTGTA TGAGGCCTTG 
ACCCATGTGT CGGAGGACTG TTTCCCTTTG CTGGACGGCT GCAGAAAGAA CAGGCAGAAA 
TGGCAGGCTC TTGCAGAACA GCAGGAGAAG ACACTGATCA ATGGTGAAAG CAGCCAGACC 
AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Ectopic expression of bovine type 5 phosphodiesterase confers a renal phenotype in Drosophila.
The Journal of biological chemistry279(9)8159-68(2004 Feb)
Broderick KE,Kean L,Dow JA,Pyne NJ,Davies SA

Binding of cGMP to both allosteric sites of cGMP-binding cGMP-specific phosphodiesterase (PDE5) is required for its phosphorylation.
The Biochemical journal329 ( Pt 3)505-10(1998 Feb)
Turko IV,Francis SH,Corbin JD

Identification of key amino acids in a conserved cGMP-binding site of cGMP-binding phosphodiesterases. A putative NKXnD motif for cGMP binding.
The Journal of biological chemistry271(36)22240-4(1996 Sep)
Turko IV,Haik TL,McAllister-Lucas LM,Burns F,Francis SH,Corbin JD

An essential aspartic acid at each of two allosteric cGMP-binding sites of a cGMP-specific phosphodiesterase.
The Journal of biological chemistry270(51)30671-9(1995 Dec)
McAllister-Lucas LM,Haik TL,Colbran JL,Sonnenburg WK,Seger D,Turko IV,Beavo JA,Francis SH,Corbin JD

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PDE5A cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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