Ago2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ago2
Entrez Gene ID	239528
Full Name	argonaute RISC catalytic subunit 2
Synonyms	1110029L17Rik,2310051F07Rik,AI225898,AL022874,AW546247,Eif2c2,Gerp95,Gm10365,mKIAA4215
General protein information	Preferred Names argonaute RISC catalytic subunit 2 Names protein argonaute-2 Piwi/Argonaute family protein meIF2C2 argonaute 2 argonaute RISC catalytic component 2 eukaryotic translation initiation factor 2C, 2 mAgo2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_153178.4	NP_694818.3	protein argonaute-2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ago2	NP_001004877.1
Pan troglodytes (chimpanzee)	EIF2C2	XP_001142838.1
Rattus norvegicus (Norway rat)	Ago2	NP_067608.1
Drosophila melanogaster (fruit fly)	AGO1	NP_001246314.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011717	XP_320795.4
Homo sapiens (human)	AGO2	NP_036286.2
Canis lupus familiaris (dog)	AGO2	XP_005628049.1
Bos taurus (cattle)	EIF2C2	NP_991363.1
Danio rerio (zebrafish)	LOC570630	XP_699226.2
Mus musculus (house mouse)	Ago2	NP_694818.3
Caenorhabditis elegans (roundworm)	alg-1	NP_001257238.1
Arabidopsis thaliana (thale cress)	AGO5	NP_850110.1
Macaca mulatta (Rhesus monkey)	LOC711873	XP_001100725.2
Caenorhabditis elegans (roundworm)	alg-2	NP_871992.1
Gallus gallus (chicken)	LOC100858102	XP_004940122.1

Gene Ontology
Bibliography

Related articles in PubMed

Control of hepatic gluconeogenesis by Argonaute2.
Yan X, Wang Z, Bishop CA, Weitkunat K, Feng X, Tarbier M, Luo J, Friedländer MR, Burkhardt R, Klaus S, Willnow TE, Poy MN
Molecular metabolism1815-24(2018 12)

Chd2 Is Necessary for Neural Circuit Development and Long-Term Memory.
Kim YJ, Khoshkhoo S, Frankowski JC, Zhu B, Abbasi S, Lee S, Wu YE, Hunt RF
Neuron100(5)1180-1193.e6(2018 12)

Hepatic Ago2-mediated RNA silencing controls energy metabolism linked to AMPK activation and obesity-associated pathophysiology.
Zhang C, Seo J, Murakami K, Salem ESB, Bernhard E, Borra VJ, Choi K, Yuan CL, Chan CC, Chen X, Huang T, Weirauch MT, Divanovic S, Qi NR, Thomas HE, Mercer CA, Siomi H, Nakamura T
Nature communications9(1)3658(2018 09)

Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells.
Ngondo RP, Cirera-Salinas D, Yu J, Wischnewski H, Bodak M, Vandormael-Pournin S, Geiselmann A, Wettstein R, Luitz J, Cohen-Tannoudji M, Ciaudo C
Stem cell reports10(2)461-476(2018 02)

Dual Strategies for Argonaute2-Mediated Biogenesis of Erythroid miRNAs Underlie Conserved Requirements for Slicing in Mammals.
Jee D, Yang JS, Park SM, Farmer DT, Wen J, Chou T, Chow A, McManus MT, Kharas MG, Lai EC
Molecular cell69(2)265-278.e6(2018 01)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ago2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ago2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22970	NM_153178.4 Latest version!	Mus musculus argonaute RISC catalytic subunit 2 (Ago2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ago2 ( NM_153178.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_694818.3 Mus musculus argonaute RISC catalytic subunit 2 (Ago2), mRNA.

Price & Availability↑

CloneID	OMu22970
Clone ID Related Accession (Same CDS sequence)	NM_153178.4
Accession Version	NM_153178.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	protein argonaute-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161581.3, BY732637.1, BE650477.1, AK220193.1, AC118640.8 and AK142854.1. On Sep 17, 2009 this sequence version replaced NM_153178.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096465.1, AB081472.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTACTCGG GAGCCGGCCC CGTTCTTGCT TCTCCTGCTC CGACAACATC ACCCATCCCA 
GGATATGCCT TCAAACCTCC ACCTCGGCCG GACTTCGGCA CCACCGGGAG AACAATCAAA 
CTACAGGCCA ATTTCTTTGA AATGGACATC CCCAAAATTG ACATCTATCA CTATGAATTG 
GACATCAAAC CTGAGAAATG CCCTCGGAGA GTGAACAGGG AAATTGTGGA GCACATGGTC 
CAGCACTTTA AAACTCAGAT CTTCGGGGAC CGGAAGCCAG TGTTTGATGG AAGGAAGAAT 
CTGTACACAG CAATGCCCCT TCCGATCGGC AGGGACAAGG TGGAGCTGGA GGTCACGCTC 
CCGGGAGAAG GCAAAGATCG CATCTTTAAG GTATCCATCA AGTGGGTGTC GTGCGTGAGC 
CTGCAGGCGT TACACGATGC ACTTTCGGGG CGGCTGCCCA GCGTCCCCTT CGAGACGATC 
CAGGCCCTGG ACGTTGTCAT GAGGCACTTA CCATCCATGA GGTACACCCC TGTTGGCCGT 
TCCTTCTTCA CTGCATCTGA AGGCTGTTCC AACCCTCTGG GTGGGGGCAG AGAAGTGTGG 
TTTGGCTTCC ATCAGTCCGT CCGACCTTCT CTTTGGAAAA TGATGCTGAA TATTGATGTA 
TCGGCAACAG CGTTTTACAA GGCACAGCCA GTGATCGAGT TTGTTTGTGA AGTTTTGGAT 
TTTAAAAGTA TTGAAGAACA ACAAAAACCT CTGACAGATT CCCAAAGGGT AAAGTTTACC 
AAAGAAATCA AAGGTCTAAA GGTGGAGATA ACGCACTGTG GTCAGATGAA GAGGAAGTAC 
CGTGTCTGCA ATGTGACCCG GCGGCCTGCC AGTCACCAAA CGTTCCCACT GCAGCAGGAG 
AGTGGGCAGA CAGTGGAGTG TACAGTGGCC CAGTACTTCA AGGACAGGCA CAAGCTGGTT 
CTGCGCTACC CCCACCTCCC GTGTTTACAA GTCGGACAGG AGCAGAAACA CACCTACCTT 
CCTTTGGAGG TCTGTAACAT AGTTGCTGGA CAGAGATGTA TAAAAAAATT AACAGACAAT 
CAGACCTCAA CCATGATCAG AGCAACTGCC AGGTCAGCAC CTGATCGCCA AGAGGAGATC 
AGCAAACTGA TGCGAAGTGC AAGTTTCAAT ACAGACCCAT ATGTTCGTGA ATTTGGAATC 
ATGGTGAAAG ATGAGATGAC AGATGTGACT GGGCGGGTTC TGCAGCCGCC CTCCATCCTC 
TACGGGGGCA GGAACAAAGC AATTGCCACC CCTGTCCAGG GTGTGTGGGA CATGCGAAAC 
AAGCAGTTCC ACACGGGCAT CGAGATCAAG GTGTGGGCCA TCGCCTGCTT CGCTCCCCAG 
CGCCAGTGTA CAGAAGTCCA TCTCAAGTCC TTCACAGAGC AACTGAGGAA GATCTCGAGA 
GATGCTGGGA TGCCGATCCA GGGTCAGCCC TGCTTCTGTA AATACGCACA GGGTGCAGAC 
AGTGTGGAGC CCATGTTCCG ACACCTGAAG AACACATACG CTGGCCTCCA GCTGGTGGTG 
GTCATCCTGC CTGGCAAAAC TCCTGTGTAT GCGGAAGTCA AGCGTGTGGG AGACACAGTG 
CTGGGGATGG CCACACAGTG CGTCCAGATG AAGAACGTGC AGAGGACCAC GCCACAGACC 
CTATCCAATC TCTGCTTAAA GATCAATGTC AAACTGGGAG GCGTCAACAA CATCCTGCTG 
CCACAGGGCA GGCCTCCAGT GTTCCAGCAA CCTGTCATCT TCCTGGGAGC CGATGTCACC 
CACCCACCAG CTGGGGATGG GAAGAAACCT TCTATTGCTG CCGTCGTGGG CAGCATGGAC 
GCACACCCCA ACCGCTACTG TGCCACCGTG CGCGTGCAGC AGCACCGACA GGAGATCATC 
CAGGACCTGG CTGCCATGGT ACGAGAGTTG CTCATTCAGT TCTACAAGTC CACCCGCTTC 
AAGCCCACCC GCATCATCTT CTACCGCGAT GGCGTCTCCG AGGGCCAGTT CCAGCAGGTT 
CTCCACCATG AGCTCCTGGC CATCAGAGAG GCCTGCATCA AGCTGGAGAA GGACTATCAG 
CCAGGAATCA CGTTCATCGT GGTGCAGAAG CGGCACCACA CACGCCTCTT CTGCACAGAC 
AAAAATGAGC GGGTTGGGAA GAGTGGGAAC ATTCCCGCAG GCACAACCGT GGACACGAAG 
ATCACCCACC CCACTGAGTT TGACTTCTAC CTGTGCAGTC ACGCCGGCAT CCAGGGGACA 
AGCCGTCCTT CCCACTACCA CGTGCTTTGG GATGACAATC GTTTTTCTTC TGATGAGCTG 
CAGATTCTGA CCTACCAGCT GTGTCACACC TACGTGCGCT GCACACGCTC TGTGTCAATA 
CCCGCGCCAG CTTACTATGC TCACCTGGTG GCCTTCCGAG CCAGGTACCA CCTGGTGGAT 
AAAGAACATG ACAGCGCTGA AGGAAGCCAT ACCTCTGGGC AGAGCAACGG GCGAGATCAC 
CAAGCGCTGG CCAAGGCGGT CCAGGTCCAC CAGGACACAC TGCGCACCAT GTACTTTGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694818.3
CDS	166..2748
Translation	MYSGAGPVLASPAPTTSPIPGYAFKPPPRPDFGTTGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	NM_153178.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus argonaute RISC catalytic subunit 2 (Ago2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153178.4

ORF Insert Sequence:

ATGTACTCGG GAGCCGGCCC CGTTCTTGCT TCTCCTGCTC CGACAACATC ACCCATCCCA 
GGATATGCCT TCAAACCTCC ACCTCGGCCG GACTTCGGCA CCACCGGGAG AACAATCAAA 
CTACAGGCCA ATTTCTTTGA AATGGACATC CCCAAAATTG ACATCTATCA CTATGAATTG 
GACATCAAAC CTGAGAAATG CCCTCGGAGA GTGAACAGGG AAATTGTGGA GCACATGGTC 
CAGCACTTTA AAACTCAGAT CTTCGGGGAC CGGAAGCCAG TGTTTGATGG AAGGAAGAAT 
CTGTACACAG CAATGCCCCT TCCGATCGGC AGGGACAAGG TGGAGCTGGA GGTCACGCTC 
CCGGGAGAAG GCAAAGATCG CATCTTTAAG GTATCCATCA AGTGGGTGTC GTGCGTGAGC 
CTGCAGGCGT TACACGATGC ACTTTCGGGG CGGCTGCCCA GCGTCCCCTT CGAGACGATC 
CAGGCCCTGG ACGTTGTCAT GAGGCACTTA CCATCCATGA GGTACACCCC TGTTGGCCGT 
TCCTTCTTCA CTGCATCTGA AGGCTGTTCC AACCCTCTGG GTGGGGGCAG AGAAGTGTGG 
TTTGGCTTCC ATCAGTCCGT CCGACCTTCT CTTTGGAAAA TGATGCTGAA TATTGATGTA 
TCGGCAACAG CGTTTTACAA GGCACAGCCA GTGATCGAGT TTGTTTGTGA AGTTTTGGAT 
TTTAAAAGTA TTGAAGAACA ACAAAAACCT CTGACAGATT CCCAAAGGGT AAAGTTTACC 
AAAGAAATCA AAGGTCTAAA GGTGGAGATA ACGCACTGTG GTCAGATGAA GAGGAAGTAC 
CGTGTCTGCA ATGTGACCCG GCGGCCTGCC AGTCACCAAA CGTTCCCACT GCAGCAGGAG 
AGTGGGCAGA CAGTGGAGTG TACAGTGGCC CAGTACTTCA AGGACAGGCA CAAGCTGGTT 
CTGCGCTACC CCCACCTCCC GTGTTTACAA GTCGGACAGG AGCAGAAACA CACCTACCTT 
CCTTTGGAGG TCTGTAACAT AGTTGCTGGA CAGAGATGTA TAAAAAAATT AACAGACAAT 
CAGACCTCAA CCATGATCAG AGCAACTGCC AGGTCAGCAC CTGATCGCCA AGAGGAGATC 
AGCAAACTGA TGCGAAGTGC AAGTTTCAAT ACAGACCCAT ATGTTCGTGA ATTTGGAATC 
ATGGTGAAAG ATGAGATGAC AGATGTGACT GGGCGGGTTC TGCAGCCGCC CTCCATCCTC 
TACGGGGGCA GGAACAAAGC AATTGCCACC CCTGTCCAGG GTGTGTGGGA CATGCGAAAC 
AAGCAGTTCC ACACGGGCAT CGAGATCAAG GTGTGGGCCA TCGCCTGCTT CGCTCCCCAG 
CGCCAGTGTA CAGAAGTCCA TCTCAAGTCC TTCACAGAGC AACTGAGGAA GATCTCGAGA 
GATGCTGGGA TGCCGATCCA GGGTCAGCCC TGCTTCTGTA AATACGCACA GGGTGCAGAC 
AGTGTGGAGC CCATGTTCCG ACACCTGAAG AACACATACG CTGGCCTCCA GCTGGTGGTG 
GTCATCCTGC CTGGCAAAAC TCCTGTGTAT GCGGAAGTCA AGCGTGTGGG AGACACAGTG 
CTGGGGATGG CCACACAGTG CGTCCAGATG AAGAACGTGC AGAGGACCAC GCCACAGACC 
CTATCCAATC TCTGCTTAAA GATCAATGTC AAACTGGGAG GCGTCAACAA CATCCTGCTG 
CCACAGGGCA GGCCTCCAGT GTTCCAGCAA CCTGTCATCT TCCTGGGAGC CGATGTCACC 
CACCCACCAG CTGGGGATGG GAAGAAACCT TCTATTGCTG CCGTCGTGGG CAGCATGGAC 
GCACACCCCA ACCGCTACTG TGCCACCGTG CGCGTGCAGC AGCACCGACA GGAGATCATC 
CAGGACCTGG CTGCCATGGT ACGAGAGTTG CTCATTCAGT TCTACAAGTC CACCCGCTTC 
AAGCCCACCC GCATCATCTT CTACCGCGAT GGCGTCTCCG AGGGCCAGTT CCAGCAGGTT 
CTCCACCATG AGCTCCTGGC CATCAGAGAG GCCTGCATCA AGCTGGAGAA GGACTATCAG 
CCAGGAATCA CGTTCATCGT GGTGCAGAAG CGGCACCACA CACGCCTCTT CTGCACAGAC 
AAAAATGAGC GGGTTGGGAA GAGTGGGAAC ATTCCCGCAG GCACAACCGT GGACACGAAG 
ATCACCCACC CCACTGAGTT TGACTTCTAC CTGTGCAGTC ACGCCGGCAT CCAGGGGACA 
AGCCGTCCTT CCCACTACCA CGTGCTTTGG GATGACAATC GTTTTTCTTC TGATGAGCTG 
CAGATTCTGA CCTACCAGCT GTGTCACACC TACGTGCGCT GCACACGCTC TGTGTCAATA 
CCCGCGCCAG CTTACTATGC TCACCTGGTG GCCTTCCGAG CCAGGTACCA CCTGGTGGAT 
AAAGAACATG ACAGCGCTGA AGGAAGCCAT ACCTCTGGGC AGAGCAACGG GCGAGATCAC 
CAAGCGCTGG CCAAGGCGGT CCAGGTCCAC CAGGACACAC TGCGCACCAT GTACTTTGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Control of hepatic gluconeogenesis by Argonaute2.
Molecular metabolism1815-24(2018 12)
Yan X,Wang Z,Bishop CA,Weitkunat K,Feng X,Tarbier M,Luo J,Friedländer MR,Burkhardt R,Klaus S,Willnow TE,Poy MN

Chd2 Is Necessary for Neural Circuit Development and Long-Term Memory.
Neuron100(5)1180-1193.e6(2018 12)
Kim YJ,Khoshkhoo S,Frankowski JC,Zhu B,Abbasi S,Lee S,Wu YE,Hunt RF

Hepatic Ago2-mediated RNA silencing controls energy metabolism linked to AMPK activation and obesity-associated pathophysiology.
Nature communications9(1)3658(2018 09)
Zhang C,Seo J,Murakami K,Salem ESB,Bernhard E,Borra VJ,Choi K,Yuan CL,Chan CC,Chen X,Huang T,Weirauch MT,Divanovic S,Qi NR,Thomas HE,Mercer CA,Siomi H,Nakamura T

Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells.
Stem cell reports10(2)461-476(2018 02)
Ngondo RP,Cirera-Salinas D,Yu J,Wischnewski H,Bodak M,Vandormael-Pournin S,Geiselmann A,Wettstein R,Luitz J,Cohen-Tannoudji M,Ciaudo C

Dual Strategies for Argonaute2-Mediated Biogenesis of Erythroid miRNAs Underlie Conserved Requirements for Slicing in Mammals.
Molecular cell69(2)265-278.e6(2018 01)
Jee D,Yang JS,Park SM,Farmer DT,Wen J,Chou T,Chow A,McManus MT,Kharas MG,Lai EC

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Ago2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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