Tmeff1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmeff1
Entrez Gene ID	230157
Full Name	transmembrane protein with EGF-like and two follistatin-like domains 1
Synonyms	A830033E11,M7365,TR-1,Tr1
General protein information	Preferred Names transmembrane protein with EGF-like and two follistatin-like domains 1 Names tomoregulin-1 transmembrane protein with EGF-like and one follistatin-like domain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 B1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006537837.2	XP_006537900.1	tomoregulin-1 isoform X1
NM_021436.2	NP_067411.1	tomoregulin-1 precursor
NM_021436.3	NP_067411.1	tomoregulin-1 isoform 2 precursor
NM_001356272.1	NP_001343201.1	tomoregulin-1 isoform 1 precursor
XM_006537836.1	XP_006537899.1	tomoregulin-1 isoform X1
XM_006537837.1	XP_006537900.1	tomoregulin-1 isoform X2
XM_006537838.1	XP_006537901.1	tomoregulin-1 isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	tmeff1	NP_001093738.2
Rattus norvegicus (Norway rat)	Tmeff1	NP_075409.1
Homo sapiens (human)	TMEFF1	NP_003683.2
Mus musculus (house mouse)	Tmeff1	NP_067411.1
Gallus gallus (chicken)	TMEFF1	XP_419072.4
Danio rerio (zebrafish)	tmeff1b	NP_001020472.1
Bos taurus (cattle)	TMEFF1	NP_001192405.1

Gene Ontology
Bibliography

Related articles in PubMed

Tomoregulin-1 prevents cardiac hypertrophy after pressure overload in mice by inhibiting TAK1-JNK pathways.
Bao D, Lu D, Liu N, Dong W, Lu YD, Qin C, Zhang LF
Disease models & mechanisms8(8)795-804(2015 Aug)

Identification of tomoregulin-1 as a novel addicsin-associated factor.
Arano T, Fujisaki S, Ikemoto MJ
Neurochemistry international7122-35(2014 May)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

GenePaint.org: an atlas of gene expression patterns in the mouse embryo.
Visel A, Thaller C, Eichele G
Nucleic acids research32(Database issue)D552-6(2004 Jan)

Identification of a Pax6-dependent epidermal growth factor family signaling source at the lateral edge of the embryonic cerebral cortex.
Assimacopoulos S, Grove EA, Ragsdale CW
The Journal of neuroscience : the official journal of the Society for Neuroscience23(16)6399-403(2003 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tmeff1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmeff1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu32877	NM_001356272.1 Latest version!	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu32877	XM_006537836.1 Latest version!	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu32878	XM_006537837.2 Latest version!	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu32879	XM_006537838.1 Latest version!	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu12337	NM_021436.3 Latest version!	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$188.30 $269.00
View Old Accession Versions >>
OMu12337	NM_021436.2	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
OMu32878	XM_006537837.1	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmeff1 ( NM_021436.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067411.1 Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), mRNA.

Price & Availability↑

CloneID	OMu12337
Clone ID Related Accession (Same CDS sequence)	NM_021436.2 , NM_021436.3
Accession Version	NM_021436.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1423843200000
Organism	Mus musculus(House mouse)
Product	tomoregulin-1 precursor
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL772151.17 and AL807771.6. On Nov 17, 2006 this sequence version replaced NM_021436.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC057598.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGA GGAGGAAGGC TCAGGAGCAG GGGCTCACAG GAAACATTCC 
AAGTGTGGAC CGTGCAAGTA CAAGGCCGAG TGTGACGAGG ATGCGGAGAA CGTTGGGTGT 
GTATGTAATA TAGACTGCAG TGGGTACAGC TTTAACCCTG TGTGCGCTTC CGACGGGAGT 
TCCTATAACA ATCCCTGTTT CGTCCGAGAA GCGTCGTGTA TAAAGCAAGA ACAGATCGAC 
ATAAGGCATC TCGGCCACTG CACAGACACA GATGATGTGA GTTTATTGGG AAAGAAGGAC 
GACGGGCTGC AGTATCGGCC AGATGTGAAA GATGCAGGTG ATGAAAGGGA AGATGTTTAT 
ATCGGAAGCC ACATGCCTTG CCCTGAAAAC CTCAATGGGT ACTGCATCCA TGGGAAATGT 
GAATTCATCT ACTCTACTCA GAAGGCCTCT TGTAGATGTG AATCTGGTTA CACTGGACAG 
CACTGTGAGA AGACGGACTT TAGTATTCTC TATGTAGTAC CAAGTAGGCA GAAGCTCACC 
CATGTTCTTA TCGCAGCCAT TATTGGAGCA GTACAGATCG CCATTATAGT AGCAATCGTC 
ATGTGCATAA CAAGGAAATG CCCCAAGAAC AATAGAGGGC GGAGGCAGAA GCAGAACCTG 
GGTCATTTTA CTTCAGACAC TTCATCCAGA ATGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067411.1
CDS	278..1396
Translation	MGAQAPLRLPAAPPLAVCGYTSVLLLFAFCLPGSRASNQPAGGGGDCPGGRGKSNCSELNLRESDIRVCDESSCKYGGVCKEDGDGLKCACQFQCHTNYIPVCGSNGDTYQNECFLRRAACKHQKDITVVARGPCYSDNGSGSGEGEEEGSGAGAHRKHSKCGPCKYKAECDEDAENVGCVCNIDCSGYSFNPVCASDGSSYNNPCFVREASCIKQEQIDIRHLGHCTDTDDVSLLGKKDDGLQYRPDVKDAGDEREDVYIGSHMPCPENLNGYCIHGKCEFIYSTQKASCRCESGYTGQHCEKTDFSILYVVPSRQKLTHVLIAAIIGAVQIAIIVAIVMCITRKCPKNNRGRRQKQNLGHFTSDTSSRMV

Target ORF information:

RefSeq Version	NM_021436.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021436.2

ORF Insert Sequence:

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGA GGAGGAAGGC TCAGGAGCAG GGGCTCACAG GAAACATTCC 
AAGTGTGGAC CGTGCAAGTA CAAGGCCGAG TGTGACGAGG ATGCGGAGAA CGTTGGGTGT 
GTATGTAATA TAGACTGCAG TGGGTACAGC TTTAACCCTG TGTGCGCTTC CGACGGGAGT 
TCCTATAACA ATCCCTGTTT CGTCCGAGAA GCGTCGTGTA TAAAGCAAGA ACAGATCGAC 
ATAAGGCATC TCGGCCACTG CACAGACACA GATGATGTGA GTTTATTGGG AAAGAAGGAC 
GACGGGCTGC AGTATCGGCC AGATGTGAAA GATGCAGGTG ATGAAAGGGA AGATGTTTAT 
ATCGGAAGCC ACATGCCTTG CCCTGAAAAC CTCAATGGGT ACTGCATCCA TGGGAAATGT 
GAATTCATCT ACTCTACTCA GAAGGCCTCT TGTAGATGTG AATCTGGTTA CACTGGACAG 
CACTGTGAGA AGACGGACTT TAGTATTCTC TATGTAGTAC CAAGTAGGCA GAAGCTCACC 
CATGTTCTTA TCGCAGCCAT TATTGGAGCA GTACAGATCG CCATTATAGT AGCAATCGTC 
ATGTGCATAA CAAGGAAATG CCCCAAGAAC AATAGAGGGC GGAGGCAGAA GCAGAACCTG 
GGTCATTTTA CTTCAGACAC TTCATCCAGA ATGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmeff1 ( NM_001356272.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001343201.1 Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu32877
Clone ID Related Accession (Same CDS sequence)	NM_001356272.1 , XM_006537836.1
Accession Version	NM_001356272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551024000000
Organism	Mus musculus(house mouse)
Product	tomoregulin-1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB619168.1, CF532023.1, CN534217.1, BU709349.1, BM947087.1 and BC057598.1. On Sep 28, 2017 this sequence version replaced XM_006537836.1. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.62375.1, SRR1660817.27830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGC AGAGGAGGAA GGCTCAGGAG CAGGGGCTCA CAGGAAACAT 
TCCAAGTGTG GACCGTGCAA GTACAAGGCC GAGTGTGACG AGGATGCGGA GAACGTTGGG 
TGTGTATGTA ATATAGACTG CAGTGGGTAC AGCTTTAACC CTGTGTGCGC TTCCGACGGG 
AGTTCCTATA ACAATCCCTG TTTCGTCCGA GAAGCGTCGT GTATAAAGCA AGAACAGATC 
GACATAAGGC ATCTCGGCCA CTGCACAGAC ACAGATGATG TGAGTTTATT GGGAAAGAAG 
GACGACGGGC TGCAGTATCG GCCAGATGTG AAAGATGCAG GTGATGAAAG GGAAGATGTT 
TATATCGGAA GCCACATGCC TTGCCCTGAA AACCTCAATG GGTACTGCAT CCATGGGAAA 
TGTGAATTCA TCTACTCTAC TCAGAAGGCC TCTTGTAGAT GTGAATCTGG TTACACTGGA 
CAGCACTGTG AGAAGACGGA CTTTAGTATT CTCTATGTAG TACCAAGTAG GCAGAAGCTC 
ACCCATGTTC TTATCGCAGC CATTATTGGA GCAGTACAGA TCGCCATTAT AGTAGCAATC 
GTCATGTGCA TAACAAGGAA ATGCCCCAAG AACAATAGAG GGCGGAGGCA GAAGCAGAAC 
CTGGGTCATT TTACTTCAGA CACTTCATCC AGAATGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343201.1
CDS	297..1418
Translation	MGAQAPLRLPAAPPLAVCGYTSVLLLFAFCLPGSRASNQPAGGGGDCPGGRGKSNCSELNLRESDIRVCDESSCKYGGVCKEDGDGLKCACQFQCHTNYIPVCGSNGDTYQNECFLRRAACKHQKDITVVARGPCYSDNGSGSGEGAEEEGSGAGAHRKHSKCGPCKYKAECDEDAENVGCVCNIDCSGYSFNPVCASDGSSYNNPCFVREASCIKQEQIDIRHLGHCTDTDDVSLLGKKDDGLQYRPDVKDAGDEREDVYIGSHMPCPENLNGYCIHGKCEFIYSTQKASCRCESGYTGQHCEKTDFSILYVVPSRQKLTHVLIAAIIGAVQIAIIVAIVMCITRKCPKNNRGRRQKQNLGHFTSDTSSRMV

Target ORF information:

RefSeq Version	NM_001356272.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356272.1

ORF Insert Sequence:

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGC AGAGGAGGAA GGCTCAGGAG CAGGGGCTCA CAGGAAACAT 
TCCAAGTGTG GACCGTGCAA GTACAAGGCC GAGTGTGACG AGGATGCGGA GAACGTTGGG 
TGTGTATGTA ATATAGACTG CAGTGGGTAC AGCTTTAACC CTGTGTGCGC TTCCGACGGG 
AGTTCCTATA ACAATCCCTG TTTCGTCCGA GAAGCGTCGT GTATAAAGCA AGAACAGATC 
GACATAAGGC ATCTCGGCCA CTGCACAGAC ACAGATGATG TGAGTTTATT GGGAAAGAAG 
GACGACGGGC TGCAGTATCG GCCAGATGTG AAAGATGCAG GTGATGAAAG GGAAGATGTT 
TATATCGGAA GCCACATGCC TTGCCCTGAA AACCTCAATG GGTACTGCAT CCATGGGAAA 
TGTGAATTCA TCTACTCTAC TCAGAAGGCC TCTTGTAGAT GTGAATCTGG TTACACTGGA 
CAGCACTGTG AGAAGACGGA CTTTAGTATT CTCTATGTAG TACCAAGTAG GCAGAAGCTC 
ACCCATGTTC TTATCGCAGC CATTATTGGA GCAGTACAGA TCGCCATTAT AGTAGCAATC 
GTCATGTGCA TAACAAGGAA ATGCCCCAAG AACAATAGAG GGCGGAGGCA GAAGCAGAAC 
CTGGGTCATT TTACTTCAGA CACTTCATCC AGAATGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmeff1 ( XM_006537836.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537899.1 Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu32877
Clone ID Related Accession (Same CDS sequence)	NM_001356272.1 , XM_006537836.1
Accession Version	XM_006537836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(House mouse)
Product	tomoregulin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGC AGAGGAGGAA GGCTCAGGAG CAGGGGCTCA CAGGAAACAT 
TCCAAGTGTG GACCGTGCAA GTACAAGGCC GAGTGTGACG AGGATGCGGA GAACGTTGGG 
TGTGTATGTA ATATAGACTG CAGTGGGTAC AGCTTTAACC CTGTGTGCGC TTCCGACGGG 
AGTTCCTATA ACAATCCCTG TTTCGTCCGA GAAGCGTCGT GTATAAAGCA AGAACAGATC 
GACATAAGGC ATCTCGGCCA CTGCACAGAC ACAGATGATG TGAGTTTATT GGGAAAGAAG 
GACGACGGGC TGCAGTATCG GCCAGATGTG AAAGATGCAG GTGATGAAAG GGAAGATGTT 
TATATCGGAA GCCACATGCC TTGCCCTGAA AACCTCAATG GGTACTGCAT CCATGGGAAA 
TGTGAATTCA TCTACTCTAC TCAGAAGGCC TCTTGTAGAT GTGAATCTGG TTACACTGGA 
CAGCACTGTG AGAAGACGGA CTTTAGTATT CTCTATGTAG TACCAAGTAG GCAGAAGCTC 
ACCCATGTTC TTATCGCAGC CATTATTGGA GCAGTACAGA TCGCCATTAT AGTAGCAATC 
GTCATGTGCA TAACAAGGAA ATGCCCCAAG AACAATAGAG GGCGGAGGCA GAAGCAGAAC 
CTGGGTCATT TTACTTCAGA CACTTCATCC AGAATGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537899.1
CDS	357..1478
Translation	MGAQAPLRLPAAPPLAVCGYTSVLLLFAFCLPGSRASNQPAGGGGDCPGGRGKSNCSELNLRESDIRVCDESSCKYGGVCKEDGDGLKCACQFQCHTNYIPVCGSNGDTYQNECFLRRAACKHQKDITVVARGPCYSDNGSGSGEGAEEEGSGAGAHRKHSKCGPCKYKAECDEDAENVGCVCNIDCSGYSFNPVCASDGSSYNNPCFVREASCIKQEQIDIRHLGHCTDTDDVSLLGKKDDGLQYRPDVKDAGDEREDVYIGSHMPCPENLNGYCIHGKCEFIYSTQKASCRCESGYTGQHCEKTDFSILYVVPSRQKLTHVLIAAIIGAVQIAIIVAIVMCITRKCPKNNRGRRQKQNLGHFTSDTSSRMV

Target ORF information:

RefSeq Version	XM_006537836.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537836.1

ORF Insert Sequence:

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGC AGAGGAGGAA GGCTCAGGAG CAGGGGCTCA CAGGAAACAT 
TCCAAGTGTG GACCGTGCAA GTACAAGGCC GAGTGTGACG AGGATGCGGA GAACGTTGGG 
TGTGTATGTA ATATAGACTG CAGTGGGTAC AGCTTTAACC CTGTGTGCGC TTCCGACGGG 
AGTTCCTATA ACAATCCCTG TTTCGTCCGA GAAGCGTCGT GTATAAAGCA AGAACAGATC 
GACATAAGGC ATCTCGGCCA CTGCACAGAC ACAGATGATG TGAGTTTATT GGGAAAGAAG 
GACGACGGGC TGCAGTATCG GCCAGATGTG AAAGATGCAG GTGATGAAAG GGAAGATGTT 
TATATCGGAA GCCACATGCC TTGCCCTGAA AACCTCAATG GGTACTGCAT CCATGGGAAA 
TGTGAATTCA TCTACTCTAC TCAGAAGGCC TCTTGTAGAT GTGAATCTGG TTACACTGGA 
CAGCACTGTG AGAAGACGGA CTTTAGTATT CTCTATGTAG TACCAAGTAG GCAGAAGCTC 
ACCCATGTTC TTATCGCAGC CATTATTGGA GCAGTACAGA TCGCCATTAT AGTAGCAATC 
GTCATGTGCA TAACAAGGAA ATGCCCCAAG AACAATAGAG GGCGGAGGCA GAAGCAGAAC 
CTGGGTCATT TTACTTCAGA CACTTCATCC AGAATGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmeff1 ( XM_006537837.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537900.1 Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu32878
Clone ID Related Accession (Same CDS sequence)	XM_006537837.2 , XM_006537837.1
Accession Version	XM_006537837.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	tomoregulin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537837.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGC AGAGGAGGAA GGCTCAGGAG CAGGGGCTCA CAGGAAACAT 
TCCAAGTGTG GACCGTGCAA GTACAAGGCC GAGTGTGACG AGGATGCGGA GAACGTTGGG 
TGTGTATGTA ATATAGACTG CAGTGGGTAC AGCTTTAACC CTGTGTGCGC TTCCGACGGG 
AGTTCCTATA ACAATCCCTG TTTCGTCCGA GAAGCGTCGT GTATAAAGCA AGAACAGATC 
GACATAAGGC ATCTCGGCCA CTGCACAGAC ACAGATGATG TGAGTTTATT GGGAAAGAAG 
GACGACGGGC TGCAGTATCG GCCAGATGTG AAAGGTGATG AAAGGGAAGA TGTTTATATC 
GGAAGCCACA TGCCTTGCCC TGAAAACCTC AATGGGTACT GCATCCATGG GAAATGTGAA 
TTCATCTACT CTACTCAGAA GGCCTCTTGT AGATGTGAAT CTGGTTACAC TGGACAGCAC 
TGTGAGAAGA CGGACTTTAG TATTCTCTAT GTAGTACCAA GTAGGCAGAA GCTCACCCAT 
GTTCTTATCG CAGCCATTAT TGGAGCAGTA CAGATCGCCA TTATAGTAGC AATCGTCATG 
TGCATAACAA GGAAATGCCC CAAGAACAAT AGAGGGCGGA GGCAGAAGCA GAACCTGGGT 
CATTTTACTT CAGACACTTC ATCCAGAATG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537900.1
CDS	400..1515
Translation	MGAQAPLRLPAAPPLAVCGYTSVLLLFAFCLPGSRASNQPAGGGGDCPGGRGKSNCSELNLRESDIRVCDESSCKYGGVCKEDGDGLKCACQFQCHTNYIPVCGSNGDTYQNECFLRRAACKHQKDITVVARGPCYSDNGSGSGEGAEEEGSGAGAHRKHSKCGPCKYKAECDEDAENVGCVCNIDCSGYSFNPVCASDGSSYNNPCFVREASCIKQEQIDIRHLGHCTDTDDVSLLGKKDDGLQYRPDVKGDEREDVYIGSHMPCPENLNGYCIHGKCEFIYSTQKASCRCESGYTGQHCEKTDFSILYVVPSRQKLTHVLIAAIIGAVQIAIIVAIVMCITRKCPKNNRGRRQKQNLGHFTSDTSSRMV

Target ORF information:

RefSeq Version	XM_006537837.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537837.2

ORF Insert Sequence:

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGC AGAGGAGGAA GGCTCAGGAG CAGGGGCTCA CAGGAAACAT 
TCCAAGTGTG GACCGTGCAA GTACAAGGCC GAGTGTGACG AGGATGCGGA GAACGTTGGG 
TGTGTATGTA ATATAGACTG CAGTGGGTAC AGCTTTAACC CTGTGTGCGC TTCCGACGGG 
AGTTCCTATA ACAATCCCTG TTTCGTCCGA GAAGCGTCGT GTATAAAGCA AGAACAGATC 
GACATAAGGC ATCTCGGCCA CTGCACAGAC ACAGATGATG TGAGTTTATT GGGAAAGAAG 
GACGACGGGC TGCAGTATCG GCCAGATGTG AAAGGTGATG AAAGGGAAGA TGTTTATATC 
GGAAGCCACA TGCCTTGCCC TGAAAACCTC AATGGGTACT GCATCCATGG GAAATGTGAA 
TTCATCTACT CTACTCAGAA GGCCTCTTGT AGATGTGAAT CTGGTTACAC TGGACAGCAC 
TGTGAGAAGA CGGACTTTAG TATTCTCTAT GTAGTACCAA GTAGGCAGAA GCTCACCCAT 
GTTCTTATCG CAGCCATTAT TGGAGCAGTA CAGATCGCCA TTATAGTAGC AATCGTCATG 
TGCATAACAA GGAAATGCCC CAAGAACAAT AGAGGGCGGA GGCAGAAGCA GAACCTGGGT 
CATTTTACTT CAGACACTTC ATCCAGAATG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmeff1 ( XM_006537837.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537900.1 Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu32878
Clone ID Related Accession (Same CDS sequence)	XM_006537837.2 , XM_006537837.1
Accession Version	XM_006537837.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	tomoregulin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGC AGAGGAGGAA GGCTCAGGAG CAGGGGCTCA CAGGAAACAT 
TCCAAGTGTG GACCGTGCAA GTACAAGGCC GAGTGTGACG AGGATGCGGA GAACGTTGGG 
TGTGTATGTA ATATAGACTG CAGTGGGTAC AGCTTTAACC CTGTGTGCGC TTCCGACGGG 
AGTTCCTATA ACAATCCCTG TTTCGTCCGA GAAGCGTCGT GTATAAAGCA AGAACAGATC 
GACATAAGGC ATCTCGGCCA CTGCACAGAC ACAGATGATG TGAGTTTATT GGGAAAGAAG 
GACGACGGGC TGCAGTATCG GCCAGATGTG AAAGGTGATG AAAGGGAAGA TGTTTATATC 
GGAAGCCACA TGCCTTGCCC TGAAAACCTC AATGGGTACT GCATCCATGG GAAATGTGAA 
TTCATCTACT CTACTCAGAA GGCCTCTTGT AGATGTGAAT CTGGTTACAC TGGACAGCAC 
TGTGAGAAGA CGGACTTTAG TATTCTCTAT GTAGTACCAA GTAGGCAGAA GCTCACCCAT 
GTTCTTATCG CAGCCATTAT TGGAGCAGTA CAGATCGCCA TTATAGTAGC AATCGTCATG 
TGCATAACAA GGAAATGCCC CAAGAACAAT AGAGGGCGGA GGCAGAAGCA GAACCTGGGT 
CATTTTACTT CAGACACTTC ATCCAGAATG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537900.1
CDS	357..1472
Translation	MGAQAPLRLPAAPPLAVCGYTSVLLLFAFCLPGSRASNQPAGGGGDCPGGRGKSNCSELNLRESDIRVCDESSCKYGGVCKEDGDGLKCACQFQCHTNYIPVCGSNGDTYQNECFLRRAACKHQKDITVVARGPCYSDNGSGSGEGAEEEGSGAGAHRKHSKCGPCKYKAECDEDAENVGCVCNIDCSGYSFNPVCASDGSSYNNPCFVREASCIKQEQIDIRHLGHCTDTDDVSLLGKKDDGLQYRPDVKGDEREDVYIGSHMPCPENLNGYCIHGKCEFIYSTQKASCRCESGYTGQHCEKTDFSILYVVPSRQKLTHVLIAAIIGAVQIAIIVAIVMCITRKCPKNNRGRRQKQNLGHFTSDTSSRMV

Target ORF information:

RefSeq Version	XM_006537837.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537837.1

ORF Insert Sequence:

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGC AGAGGAGGAA GGCTCAGGAG CAGGGGCTCA CAGGAAACAT 
TCCAAGTGTG GACCGTGCAA GTACAAGGCC GAGTGTGACG AGGATGCGGA GAACGTTGGG 
TGTGTATGTA ATATAGACTG CAGTGGGTAC AGCTTTAACC CTGTGTGCGC TTCCGACGGG 
AGTTCCTATA ACAATCCCTG TTTCGTCCGA GAAGCGTCGT GTATAAAGCA AGAACAGATC 
GACATAAGGC ATCTCGGCCA CTGCACAGAC ACAGATGATG TGAGTTTATT GGGAAAGAAG 
GACGACGGGC TGCAGTATCG GCCAGATGTG AAAGGTGATG AAAGGGAAGA TGTTTATATC 
GGAAGCCACA TGCCTTGCCC TGAAAACCTC AATGGGTACT GCATCCATGG GAAATGTGAA 
TTCATCTACT CTACTCAGAA GGCCTCTTGT AGATGTGAAT CTGGTTACAC TGGACAGCAC 
TGTGAGAAGA CGGACTTTAG TATTCTCTAT GTAGTACCAA GTAGGCAGAA GCTCACCCAT 
GTTCTTATCG CAGCCATTAT TGGAGCAGTA CAGATCGCCA TTATAGTAGC AATCGTCATG 
TGCATAACAA GGAAATGCCC CAAGAACAAT AGAGGGCGGA GGCAGAAGCA GAACCTGGGT 
CATTTTACTT CAGACACTTC ATCCAGAATG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmeff1 ( XM_006537838.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537901.1 Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu32879
Clone ID Related Accession (Same CDS sequence)	XM_006537838.1
Accession Version	XM_006537838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	tomoregulin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGA GGAGGAAGGC TCAGGAGCAG GGGCTCACAG GAAACATTCC 
AAGTGTGGAC CGTGCAAGTA CAAGGCCGAG TGTGACGAGG ATGCGGAGAA CGTTGGGTGT 
GTATGTAATA TAGACTGCAG TGGGTACAGC TTTAACCCTG TGTGCGCTTC CGACGGGAGT 
TCCTATAACA ATCCCTGTTT CGTCCGAGAA GCGTCGTGTA TAAAGCAAGA ACAGATCGAC 
ATAAGGCATC TCGGCCACTG CACAGACACA GATGATGTGA GTTTATTGGG AAAGAAGGAC 
GACGGGCTGC AGTATCGGCC AGATGTGAAA GGTGATGAAA GGGAAGATGT TTATATCGGA 
AGCCACATGC CTTGCCCTGA AAACCTCAAT GGGTACTGCA TCCATGGGAA ATGTGAATTC 
ATCTACTCTA CTCAGAAGGC CTCTTGTAGA TGTGAATCTG GTTACACTGG ACAGCACTGT 
GAGAAGACGG ACTTTAGTAT TCTCTATGTA GTACCAAGTA GGCAGAAGCT CACCCATGTT 
CTTATCGCAG CCATTATTGG AGCAGTACAG ATCGCCATTA TAGTAGCAAT CGTCATGTGC 
ATAACAAGGA AATGCCCCAA GAACAATAGA GGGCGGAGGC AGAAGCAGAA CCTGGGTCAT 
TTTACTTCAG ACACTTCATC CAGAATGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537901.1
CDS	357..1469
Translation	MGAQAPLRLPAAPPLAVCGYTSVLLLFAFCLPGSRASNQPAGGGGDCPGGRGKSNCSELNLRESDIRVCDESSCKYGGVCKEDGDGLKCACQFQCHTNYIPVCGSNGDTYQNECFLRRAACKHQKDITVVARGPCYSDNGSGSGEGEEEGSGAGAHRKHSKCGPCKYKAECDEDAENVGCVCNIDCSGYSFNPVCASDGSSYNNPCFVREASCIKQEQIDIRHLGHCTDTDDVSLLGKKDDGLQYRPDVKGDEREDVYIGSHMPCPENLNGYCIHGKCEFIYSTQKASCRCESGYTGQHCEKTDFSILYVVPSRQKLTHVLIAAIIGAVQIAIIVAIVMCITRKCPKNNRGRRQKQNLGHFTSDTSSRMV

Target ORF information:

RefSeq Version	XM_006537838.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537838.1

ORF Insert Sequence:

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGA GGAGGAAGGC TCAGGAGCAG GGGCTCACAG GAAACATTCC 
AAGTGTGGAC CGTGCAAGTA CAAGGCCGAG TGTGACGAGG ATGCGGAGAA CGTTGGGTGT 
GTATGTAATA TAGACTGCAG TGGGTACAGC TTTAACCCTG TGTGCGCTTC CGACGGGAGT 
TCCTATAACA ATCCCTGTTT CGTCCGAGAA GCGTCGTGTA TAAAGCAAGA ACAGATCGAC 
ATAAGGCATC TCGGCCACTG CACAGACACA GATGATGTGA GTTTATTGGG AAAGAAGGAC 
GACGGGCTGC AGTATCGGCC AGATGTGAAA GGTGATGAAA GGGAAGATGT TTATATCGGA 
AGCCACATGC CTTGCCCTGA AAACCTCAAT GGGTACTGCA TCCATGGGAA ATGTGAATTC 
ATCTACTCTA CTCAGAAGGC CTCTTGTAGA TGTGAATCTG GTTACACTGG ACAGCACTGT 
GAGAAGACGG ACTTTAGTAT TCTCTATGTA GTACCAAGTA GGCAGAAGCT CACCCATGTT 
CTTATCGCAG CCATTATTGG AGCAGTACAG ATCGCCATTA TAGTAGCAAT CGTCATGTGC 
ATAACAAGGA AATGCCCCAA GAACAATAGA GGGCGGAGGC AGAAGCAGAA CCTGGGTCAT 
TTTACTTCAG ACACTTCATC CAGAATGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmeff1 ( NM_021436.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067411.1 Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu12337
Clone ID Related Accession (Same CDS sequence)	NM_021436.2 , NM_021436.3
Accession Version	NM_021436.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551110400000
Organism	Mus musculus(house mouse)
Product	tomoregulin-1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB619168.1, BC057598.1, AW491640.1, CN534217.1, BU709349.1 and BM947087.1. On Sep 28, 2017 this sequence version replaced NM_021436.2. ##Evidence-Data-START## Transcript exon combination :: BC057598.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGA GGAGGAAGGC TCAGGAGCAG GGGCTCACAG GAAACATTCC 
AAGTGTGGAC CGTGCAAGTA CAAGGCCGAG TGTGACGAGG ATGCGGAGAA CGTTGGGTGT 
GTATGTAATA TAGACTGCAG TGGGTACAGC TTTAACCCTG TGTGCGCTTC CGACGGGAGT 
TCCTATAACA ATCCCTGTTT CGTCCGAGAA GCGTCGTGTA TAAAGCAAGA ACAGATCGAC 
ATAAGGCATC TCGGCCACTG CACAGACACA GATGATGTGA GTTTATTGGG AAAGAAGGAC 
GACGGGCTGC AGTATCGGCC AGATGTGAAA GATGCAGGTG ATGAAAGGGA AGATGTTTAT 
ATCGGAAGCC ACATGCCTTG CCCTGAAAAC CTCAATGGGT ACTGCATCCA TGGGAAATGT 
GAATTCATCT ACTCTACTCA GAAGGCCTCT TGTAGATGTG AATCTGGTTA CACTGGACAG 
CACTGTGAGA AGACGGACTT TAGTATTCTC TATGTAGTAC CAAGTAGGCA GAAGCTCACC 
CATGTTCTTA TCGCAGCCAT TATTGGAGCA GTACAGATCG CCATTATAGT AGCAATCGTC 
ATGTGCATAA CAAGGAAATG CCCCAAGAAC AATAGAGGGC GGAGGCAGAA GCAGAACCTG 
GGTCATTTTA CTTCAGACAC TTCATCCAGA ATGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067411.1
CDS	297..1415
Translation	MGAQAPLRLPAAPPLAVCGYTSVLLLFAFCLPGSRASNQPAGGGGDCPGGRGKSNCSELNLRESDIRVCDESSCKYGGVCKEDGDGLKCACQFQCHTNYIPVCGSNGDTYQNECFLRRAACKHQKDITVVARGPCYSDNGSGSGEGEEEGSGAGAHRKHSKCGPCKYKAECDEDAENVGCVCNIDCSGYSFNPVCASDGSSYNNPCFVREASCIKQEQIDIRHLGHCTDTDDVSLLGKKDDGLQYRPDVKDAGDEREDVYIGSHMPCPENLNGYCIHGKCEFIYSTQKASCRCESGYTGQHCEKTDFSILYVVPSRQKLTHVLIAAIIGAVQIAIIVAIVMCITRKCPKNNRGRRQKQNLGHFTSDTSSRMV

Target ORF information:

RefSeq Version	NM_021436.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein with EGF-like and two follistatin-like domains 1 (Tmeff1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021436.3

ORF Insert Sequence:

ATGGGCGCCC AGGCGCCGCT CCGGCTGCCG GCCGCGCCCC CGCTCGCCGT CTGCGGCTAC 
ACGTCGGTGC TTCTGCTCTT CGCCTTCTGC CTGCCCGGGA GCCGCGCGTC CAACCAGCCC 
GCGGGCGGCG GCGGGGACTG TCCGGGAGGC AGAGGCAAGA GCAACTGCTC AGAATTAAAT 
CTGAGAGAAT CTGACATCAG AGTGTGTGAT GAATCATCTT GTAAATATGG AGGTGTCTGC 
AAAGAAGATG GAGATGGTTT GAAATGCGCA TGCCAGTTTC AGTGCCATAC AAATTACATC 
CCTGTCTGTG GGTCAAACGG GGACACCTAC CAGAATGAGT GCTTTCTCAG GAGGGCTGCT 
TGCAAGCACC AGAAAGACAT AACCGTGGTG GCGCGTGGAC CTTGCTACTC TGATAACGGC 
TCTGGATCTG GAGAAGGAGA GGAGGAAGGC TCAGGAGCAG GGGCTCACAG GAAACATTCC 
AAGTGTGGAC CGTGCAAGTA CAAGGCCGAG TGTGACGAGG ATGCGGAGAA CGTTGGGTGT 
GTATGTAATA TAGACTGCAG TGGGTACAGC TTTAACCCTG TGTGCGCTTC CGACGGGAGT 
TCCTATAACA ATCCCTGTTT CGTCCGAGAA GCGTCGTGTA TAAAGCAAGA ACAGATCGAC 
ATAAGGCATC TCGGCCACTG CACAGACACA GATGATGTGA GTTTATTGGG AAAGAAGGAC 
GACGGGCTGC AGTATCGGCC AGATGTGAAA GATGCAGGTG ATGAAAGGGA AGATGTTTAT 
ATCGGAAGCC ACATGCCTTG CCCTGAAAAC CTCAATGGGT ACTGCATCCA TGGGAAATGT 
GAATTCATCT ACTCTACTCA GAAGGCCTCT TGTAGATGTG AATCTGGTTA CACTGGACAG 
CACTGTGAGA AGACGGACTT TAGTATTCTC TATGTAGTAC CAAGTAGGCA GAAGCTCACC 
CATGTTCTTA TCGCAGCCAT TATTGGAGCA GTACAGATCG CCATTATAGT AGCAATCGTC 
ATGTGCATAA CAAGGAAATG CCCCAAGAAC AATAGAGGGC GGAGGCAGAA GCAGAACCTG 
GGTCATTTTA CTTCAGACAC TTCATCCAGA ATGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tomoregulin-1 prevents cardiac hypertrophy after pressure overload in mice by inhibiting TAK1-JNK pathways.
Disease models & mechanisms8(8)795-804(2015 Aug)
Bao D,Lu D,Liu N,Dong W,Lu YD,Qin C,Zhang LF

Identification of tomoregulin-1 as a novel addicsin-associated factor.
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