Ambra1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ambra1
Entrez Gene ID	228361
Full Name	autophagy/beclin 1 regulator 1
Synonyms	2310079H06Rik,A130023A14,AA474864,AV021921,D030051N19Rik,mKIAA1736
General protein information	Preferred Names autophagy/beclin 1 regulator 1 Names activating molecule in BECN1-regulated autophagy protein 1 activating molecule in Beclin1-regulated autophagy
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_172669.3	NP_766257.3	activating molecule in BECN1-regulated autophagy protein 1 isoform 1
XM_006499252.1	XP_006499315.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_006499253.2	XP_006499316.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
NM_001080754.1	NP_001074223.1	activating molecule in BECN1-regulated autophagy protein 1 isoform 2
XM_006499253.1	XP_006499316.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
XM_006499252.2	XP_006499315.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ambra1	NP_766257.3
Gallus gallus (chicken)	AMBRA1	XP_004941659.1
Danio rerio (zebrafish)	ambra1	XP_002667715.3
Canis lupus familiaris (dog)	AMBRA1	XP_005631347.1
Rattus norvegicus (Norway rat)	Ambra1	NP_001127813.1
Xenopus tropicalis (tropical clawed frog)	ambra1	XP_004913444.1
Homo sapiens (human)	AMBRA1	NP_060219.2
Pan troglodytes (chimpanzee)	AMBRA1	XP_508396.2
Macaca mulatta (Rhesus monkey)	AMBRA1	XP_001112256.1
Bos taurus (cattle)	AMBRA1	NP_001029694.2

Gene Ontology
Bibliography

Related articles in PubMed

Mitsugumin 53 promotes mitochondrial autophagy through regulating Ambra1 expression in C2C12 myoblast cells.
Lijie G, Yueyue Z, Nan Z, Ling W, Xuan W, Weijie Y
Cell biology international43(3)290-298(2019 Mar)

GIT1 contributes to autophagy in osteoclast through disruption of the binding of Beclin1 and Bcl2 under starvation condition.
Zhao SJ, Kong FQ, Cai W, Xu T, Zhou ZM, Wang ZB, Xu AD, Yang YQ, Chen J, Tang PY, Wang Q, Cheng L, Luo YJ, Zhou Z, Li LW, Huang YF, Zhao X, Yin GY, Xue MX, Fan J
Cell death & disease9(12)1195(2018 12)

Ambra1 Shapes Hippocampal Inhibition/Excitation Balance: Role in Neurodevelopmental Disorders.
Nobili A, Krashia P, Cordella A, La Barbera L, Dell'Acqua MC, Caruso A, Pignataro A, Marino R, Sciarra F, Biamonte F, Scattoni ML, Ammassari-Teule M, Cecconi F, Berretta N, Keller F, Mercuri NB, D'Amelio M
Molecular neurobiology55(10)7921-7940(2018 Oct)

Microglia ablation alleviates myelin-associated catatonic signs in mice.
Janova H, Arinrad S, Balmuth E, Mitjans M, Hertel J, Habes M, Bittner RA, Pan H, Goebbels S, Begemann M, Gerwig UC, Langner S, Werner HB, Kittel-Schneider S, Homuth G, Davatzikos C, Völzke H, West BL, Reif A, Grabe HJ, Boretius S, Ehrenreich H, Nave KA
The Journal of clinical investigation128(2)734-745(2018 02)

Effects of caloric restriction on neuropathic pain, peripheral nerve degeneration and inflammation in normometabolic and autophagy defective prediabetic Ambra1 mice.
Coccurello R, Nazio F, Rossi C, De Angelis F, Vacca V, Giacovazzo G, Procacci P, Magnaghi V, Ciavardelli D, Marinelli S
PloS one13(12)e0208596(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ambra1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ambra1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18657	NM_172669.3 Latest version!	Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu18871	NM_001080754.1 Latest version!	Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu30300	XM_006499252.2 Latest version!	Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu30301	XM_006499253.2 Latest version!	Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OMu30301	XM_006499253.1	Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu30300	XM_006499252.1	Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ambra1 ( NM_172669.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766257.3 Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18657
Clone ID Related Accession (Same CDS sequence)	NM_172669.3
Accession Version	NM_172669.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Mus musculus(house mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ150588.1, AK083596.1, AK129433.1 and BC032293.1. On Jan 24, 2007 this sequence version replaced NM_172669.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK155513.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCTCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGTGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTT TGAGGGCATG CCACCAAGTG GCAACCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGTCAGC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT ACTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCCGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACT TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACGTG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG CTGCAGCCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ACGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCCATC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGGAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACAG GGGGGAAGCT GCAGGCCCTA GTGGAGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766257.3
CDS	362..4264
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPSSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLPSEPGQLAERALSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	NM_172669.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172669.3

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCTCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGTGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTT TGAGGGCATG CCACCAAGTG GCAACCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGTCAGC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT ACTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCCGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACT TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACGTG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG CTGCAGCCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ACGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCCATC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGGAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACAG GGGGGAAGCT GCAGGCCCTA GTGGAGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( NM_001080754.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074223.1 Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18871
Clone ID Related Accession (Same CDS sequence)	NM_001080754.1
Accession Version	NM_001080754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Mus musculus(house mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ150588.1, AK169280.1 and BC032293.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032293.1, SRR1660813.114170.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCTCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GTGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTTTGA GGGCATGCCA 
CCAAGTGGCA ACCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCAAG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AATCGGCTTC GTTCTTCCAC CTCCTCTTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT 
CCCCGAAATG CCCGAATGTC TGCACCTTCA CTTGGACGAT TTGTTCCAAG GCGTTTTTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCATTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTAA TTGGAGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC AGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTTCCAG AAATCAGTAA TGCCTCTGTG AATGTCCTGG TACAGAATTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGTCAGCTCC TGGCAGCCTT CATCCCGAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ATTCCCTGGC CCCCCATAAC 
TTGGGGGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCTGT AAGCCTGTCC 
CCGATGGGCC GATACGTTAT GGTGGGCTTG GCCTCACGAA GGATCCTACT GCACCCCTCC 
ACAGAGCACA TGGTAGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG TGAGACTTCC 
ATGAGGAGAG TTTTCAATGT CCTTTACCCC ATGCCTGCAG ACCAACGGAG ACACGTCAGC 
ATCAACTCTG CCCGTTGGCT ACCCGAGCCA GGGCTTGGTT TGGCCTATGG CACCAACAAA 
GGAGACTTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GCATCGAGTA CTACTGGGAC 
CAGCTAAGTG AAACAGTCTT CACTGTCCAC TCAAGTAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACGTGGCG GACAGACAGA GACATGGGTT TGATGAATGC AATTGGGCTG 
CAGCCTCGGA ACCCAACCAC CTCTGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTCCAG 
TTGCAGAACG CTGAAACACA GACTGAGAGG GAGGAAGAGG AACCAGGGGC AGCATCTTCA 
GGTCCTGGTG AAGGCGAGGG CTCAGAATAT GGTGGCAGTG GGGAAGATGC CCTCAGCAGG 
ATCCAGAGGC TGATGGCCGA GGGTGGCATG ACAGCTGTGG TACAACGGGA ACAGAGCACC 
ACCATGGCCT CCATGGGTGG CTTTGGCAAC AACATCATCG TTAGCCACCG CATTCACCGC 
AGCTCCCAGA CAGGCACAGA GTCAGGGGCT GCTCGTACCT CCTCACCCCA GCCCTCCACC 
TCTCGAGGAC TGCCATCAGA ACCTGGGCAA CTGGCAGAGC GAGCGCTAAG CCCCCGGACA 
GCCTCCTGGG ACCAGCCTAG TACCTCTGGG AGGGAACTAC CCCAGCCAGC CCTGTCCTCT 
TCCTCCCCTG TCCCCATTCC AGTCCCCCTT GCCAGCAATG AGGGACCAAC CATGCACTGC 
AATGTGACCA ATAACAGTCA CCTTCCTGAG GGTGATGGTA GCAACAGGGG GGAAGCTGCA 
GGCCCTAGTG GAGAGCCACA GAACCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074223.1
CDS	362..3991
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPSSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLPSEPGQLAERALSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	NM_001080754.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080754.1

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCTCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GTGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTTTGA GGGCATGCCA 
CCAAGTGGCA ACCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCAAG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AATCGGCTTC GTTCTTCCAC CTCCTCTTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT 
CCCCGAAATG CCCGAATGTC TGCACCTTCA CTTGGACGAT TTGTTCCAAG GCGTTTTTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCATTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTAA TTGGAGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC AGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTTCCAG AAATCAGTAA TGCCTCTGTG AATGTCCTGG TACAGAATTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGTCAGCTCC TGGCAGCCTT CATCCCGAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ATTCCCTGGC CCCCCATAAC 
TTGGGGGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCTGT AAGCCTGTCC 
CCGATGGGCC GATACGTTAT GGTGGGCTTG GCCTCACGAA GGATCCTACT GCACCCCTCC 
ACAGAGCACA TGGTAGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG TGAGACTTCC 
ATGAGGAGAG TTTTCAATGT CCTTTACCCC ATGCCTGCAG ACCAACGGAG ACACGTCAGC 
ATCAACTCTG CCCGTTGGCT ACCCGAGCCA GGGCTTGGTT TGGCCTATGG CACCAACAAA 
GGAGACTTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GCATCGAGTA CTACTGGGAC 
CAGCTAAGTG AAACAGTCTT CACTGTCCAC TCAAGTAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACGTGGCG GACAGACAGA GACATGGGTT TGATGAATGC AATTGGGCTG 
CAGCCTCGGA ACCCAACCAC CTCTGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTCCAG 
TTGCAGAACG CTGAAACACA GACTGAGAGG GAGGAAGAGG AACCAGGGGC AGCATCTTCA 
GGTCCTGGTG AAGGCGAGGG CTCAGAATAT GGTGGCAGTG GGGAAGATGC CCTCAGCAGG 
ATCCAGAGGC TGATGGCCGA GGGTGGCATG ACAGCTGTGG TACAACGGGA ACAGAGCACC 
ACCATGGCCT CCATGGGTGG CTTTGGCAAC AACATCATCG TTAGCCACCG CATTCACCGC 
AGCTCCCAGA CAGGCACAGA GTCAGGGGCT GCTCGTACCT CCTCACCCCA GCCCTCCACC 
TCTCGAGGAC TGCCATCAGA ACCTGGGCAA CTGGCAGAGC GAGCGCTAAG CCCCCGGACA 
GCCTCCTGGG ACCAGCCTAG TACCTCTGGG AGGGAACTAC CCCAGCCAGC CCTGTCCTCT 
TCCTCCCCTG TCCCCATTCC AGTCCCCCTT GCCAGCAATG AGGGACCAAC CATGCACTGC 
AATGTGACCA ATAACAGTCA CCTTCCTGAG GGTGATGGTA GCAACAGGGG GGAAGCTGCA 
GGCCCTAGTG GAGAGCCACA GAACCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_006499252.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499315.1 Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.

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CloneID	OMu30300
Clone ID Related Accession (Same CDS sequence)	XM_006499252.1 , XM_006499252.2
Accession Version	XM_006499252.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCTCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGTGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTT TGAGGGCATG CCACCAAGTG GCAACCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGATTAT CTCCTGCTGC ATACTACGCC 
CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC 
CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA 
GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC 
CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCTTCACTTG GACGATTTGT TCCAAGGCGT 
TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAACGTGG ACAGCCTGGC 
TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG 
TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACT 
AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCTGTGAATG TCCTGGTACA GAATTGCAAG 
ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGTC AGCTCCTGGC AGCCTTCATC 
CCGAGCAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC 
CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC 
CTGTCCCCGA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTACTGCAC 
CCCTCCACAG AGCACATGGT AGCCCAGGTC TTCAGGCTGC AACAGGCCCA TGGTGGTGAG 
ACTTCCATGA GGAGAGTTTT CAATGTCCTT TACCCCATGC CTGCAGACCA ACGGAGACAC 
GTCAGCATCA ACTCTGCCCG TTGGCTACCC GAGCCAGGGC TTGGTTTGGC CTATGGCACC 
AACAAAGGAG ACTTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT CGAGTACTAC 
TGGGACCAGC TAAGTGAAAC AGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG 
CCTGGAACCA GCAGAGCCAC GTGGCGGACA GACAGAGACA TGGGTTTGAT GAATGCAATT 
GGGCTGCAGC CTCGGAACCC AACCACCTCT GTGACATCTC AGGGCACCCA GACTCTGGCC 
CTCCAGTTGC AGAACGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGGCAGCA 
TCTTCAGGTC CTGGTGAAGG CGAGGGCTCA GAATATGGTG GCAGTGGGGA AGATGCCCTC 
AGCAGGATCC AGAGGCTGAT GGCCGAGGGT GGCATGACAG CTGTGGTACA ACGGGAACAG 
AGCACCACCA TGGCCTCCAT GGGTGGCTTT GGCAACAACA TCATCGTTAG CCACCGCATT 
CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC 
TCCACCTCTC GAGGACTGCC ATCAGAACCT GGGCAACTGG CAGAGCGAGC GCTAAGCCCC 
CGGACAGCCT CCTGGGACCA GCCTAGTACC TCTGGGAGGG AACTACCCCA GCCAGCCCTG 
TCCTCTTCCT CCCCTGTCCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG 
CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATGGTAGCAA CAGGGGGGAA 
GCTGCAGGCC CTAGTGGAGA GCCACAGAAC CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499315.1
CDS	389..4204
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPSSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLPSEPGQLAERALSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	XM_006499252.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499252.1

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCTCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGTGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTT TGAGGGCATG CCACCAAGTG GCAACCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGATTAT CTCCTGCTGC ATACTACGCC 
CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC 
CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA 
GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC 
CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCTTCACTTG GACGATTTGT TCCAAGGCGT 
TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAACGTGG ACAGCCTGGC 
TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG 
TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACT 
AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCTGTGAATG TCCTGGTACA GAATTGCAAG 
ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGTC AGCTCCTGGC AGCCTTCATC 
CCGAGCAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC 
CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC 
CTGTCCCCGA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTACTGCAC 
CCCTCCACAG AGCACATGGT AGCCCAGGTC TTCAGGCTGC AACAGGCCCA TGGTGGTGAG 
ACTTCCATGA GGAGAGTTTT CAATGTCCTT TACCCCATGC CTGCAGACCA ACGGAGACAC 
GTCAGCATCA ACTCTGCCCG TTGGCTACCC GAGCCAGGGC TTGGTTTGGC CTATGGCACC 
AACAAAGGAG ACTTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT CGAGTACTAC 
TGGGACCAGC TAAGTGAAAC AGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG 
CCTGGAACCA GCAGAGCCAC GTGGCGGACA GACAGAGACA TGGGTTTGAT GAATGCAATT 
GGGCTGCAGC CTCGGAACCC AACCACCTCT GTGACATCTC AGGGCACCCA GACTCTGGCC 
CTCCAGTTGC AGAACGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGGCAGCA 
TCTTCAGGTC CTGGTGAAGG CGAGGGCTCA GAATATGGTG GCAGTGGGGA AGATGCCCTC 
AGCAGGATCC AGAGGCTGAT GGCCGAGGGT GGCATGACAG CTGTGGTACA ACGGGAACAG 
AGCACCACCA TGGCCTCCAT GGGTGGCTTT GGCAACAACA TCATCGTTAG CCACCGCATT 
CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC 
TCCACCTCTC GAGGACTGCC ATCAGAACCT GGGCAACTGG CAGAGCGAGC GCTAAGCCCC 
CGGACAGCCT CCTGGGACCA GCCTAGTACC TCTGGGAGGG AACTACCCCA GCCAGCCCTG 
TCCTCTTCCT CCCCTGTCCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG 
CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATGGTAGCAA CAGGGGGGAA 
GCTGCAGGCC CTAGTGGAGA GCCACAGAAC CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_006499252.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499315.1 Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30300
Clone ID Related Accession (Same CDS sequence)	XM_006499252.1 , XM_006499252.2
Accession Version	XM_006499252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCTCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGTGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTT TGAGGGCATG CCACCAAGTG GCAACCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGATTAT CTCCTGCTGC ATACTACGCC 
CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC 
CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA 
GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC 
CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCTTCACTTG GACGATTTGT TCCAAGGCGT 
TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAACGTGG ACAGCCTGGC 
TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG 
TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACT 
AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCTGTGAATG TCCTGGTACA GAATTGCAAG 
ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGTC AGCTCCTGGC AGCCTTCATC 
CCGAGCAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC 
CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC 
CTGTCCCCGA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTACTGCAC 
CCCTCCACAG AGCACATGGT AGCCCAGGTC TTCAGGCTGC AACAGGCCCA TGGTGGTGAG 
ACTTCCATGA GGAGAGTTTT CAATGTCCTT TACCCCATGC CTGCAGACCA ACGGAGACAC 
GTCAGCATCA ACTCTGCCCG TTGGCTACCC GAGCCAGGGC TTGGTTTGGC CTATGGCACC 
AACAAAGGAG ACTTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT CGAGTACTAC 
TGGGACCAGC TAAGTGAAAC AGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG 
CCTGGAACCA GCAGAGCCAC GTGGCGGACA GACAGAGACA TGGGTTTGAT GAATGCAATT 
GGGCTGCAGC CTCGGAACCC AACCACCTCT GTGACATCTC AGGGCACCCA GACTCTGGCC 
CTCCAGTTGC AGAACGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGGCAGCA 
TCTTCAGGTC CTGGTGAAGG CGAGGGCTCA GAATATGGTG GCAGTGGGGA AGATGCCCTC 
AGCAGGATCC AGAGGCTGAT GGCCGAGGGT GGCATGACAG CTGTGGTACA ACGGGAACAG 
AGCACCACCA TGGCCTCCAT GGGTGGCTTT GGCAACAACA TCATCGTTAG CCACCGCATT 
CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC 
TCCACCTCTC GAGGACTGCC ATCAGAACCT GGGCAACTGG CAGAGCGAGC GCTAAGCCCC 
CGGACAGCCT CCTGGGACCA GCCTAGTACC TCTGGGAGGG AACTACCCCA GCCAGCCCTG 
TCCTCTTCCT CCCCTGTCCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG 
CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATGGTAGCAA CAGGGGGGAA 
GCTGCAGGCC CTAGTGGAGA GCCACAGAAC CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499315.1
CDS	342..4157
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPSSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLPSEPGQLAERALSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	XM_006499252.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499252.2

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG 
TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCTCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT 
CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGTGAGGC TAGCTCTTCC 
TGGCACGTCT CCACTACCTT TGAGGGCATG CCACCAAGTG GCAACCAGCT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGATTAT CTCCTGCTGC ATACTACGCC 
CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC 
CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA 
GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC 
CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCTTCACTTG GACGATTTGT TCCAAGGCGT 
TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAACGTGG ACAGCCTGGC 
TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG 
TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACT 
AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCTGTGAATG TCCTGGTACA GAATTGCAAG 
ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGTC AGCTCCTGGC AGCCTTCATC 
CCGAGCAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC 
CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC 
CTGTCCCCGA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTACTGCAC 
CCCTCCACAG AGCACATGGT AGCCCAGGTC TTCAGGCTGC AACAGGCCCA TGGTGGTGAG 
ACTTCCATGA GGAGAGTTTT CAATGTCCTT TACCCCATGC CTGCAGACCA ACGGAGACAC 
GTCAGCATCA ACTCTGCCCG TTGGCTACCC GAGCCAGGGC TTGGTTTGGC CTATGGCACC 
AACAAAGGAG ACTTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT CGAGTACTAC 
TGGGACCAGC TAAGTGAAAC AGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG 
CCTGGAACCA GCAGAGCCAC GTGGCGGACA GACAGAGACA TGGGTTTGAT GAATGCAATT 
GGGCTGCAGC CTCGGAACCC AACCACCTCT GTGACATCTC AGGGCACCCA GACTCTGGCC 
CTCCAGTTGC AGAACGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGGCAGCA 
TCTTCAGGTC CTGGTGAAGG CGAGGGCTCA GAATATGGTG GCAGTGGGGA AGATGCCCTC 
AGCAGGATCC AGAGGCTGAT GGCCGAGGGT GGCATGACAG CTGTGGTACA ACGGGAACAG 
AGCACCACCA TGGCCTCCAT GGGTGGCTTT GGCAACAACA TCATCGTTAG CCACCGCATT 
CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC 
TCCACCTCTC GAGGACTGCC ATCAGAACCT GGGCAACTGG CAGAGCGAGC GCTAAGCCCC 
CGGACAGCCT CCTGGGACCA GCCTAGTACC TCTGGGAGGG AACTACCCCA GCCAGCCCTG 
TCCTCTTCCT CCCCTGTCCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG 
CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATGGTAGCAA CAGGGGGGAA 
GCTGCAGGCC CTAGTGGAGA GCCACAGAAC CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_006499253.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499316.1 Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu30301
Clone ID Related Accession (Same CDS sequence)	XM_006499253.2 , XM_006499253.1
Accession Version	XM_006499253.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499253.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCTCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GTGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTTTGA GGGCATGCCA 
CCAAGTGGCA ACCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGTCAGC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT ACTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCCGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACT TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACGTG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG CTGCAGCCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ACGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCCATC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGGAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACAG GGGGGAAGCT GCAGGCCCTA GTGGAGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499316.1
CDS	352..3894
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPSSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLPSEPGQLAERALSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	XM_006499253.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499253.2

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCTCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GTGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTTTGA GGGCATGCCA 
CCAAGTGGCA ACCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGTCAGC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT ACTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCCGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACT TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACGTG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG CTGCAGCCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ACGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCCATC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGGAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACAG GGGGGAAGCT GCAGGCCCTA GTGGAGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_006499253.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499316.1 Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.

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CloneID	OMu30301
Clone ID Related Accession (Same CDS sequence)	XM_006499253.2 , XM_006499253.1
Accession Version	XM_006499253.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCTCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GTGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTTTGA GGGCATGCCA 
CCAAGTGGCA ACCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGTCAGC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT ACTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCCGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACT TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACGTG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG CTGCAGCCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ACGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCCATC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGGAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACAG GGGGGAAGCT GCAGGCCCTA GTGGAGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499316.1
CDS	388..3930
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPSSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLPSEPGQLAERALSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR

Target ORF information:

RefSeq Version	XM_006499253.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499253.1

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA 
GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG 
GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA 
TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCTCGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCTG GTGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTTTGA GGGCATGCCA 
CCAAGTGGCA ACCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT 
TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT 
GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGTCAGC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT ACTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC 
CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCCGAG 
CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACT TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACGTG GCGGACAGAC 
AGAGACATGG GTTTGATGAA TGCAATTGGG CTGCAGCCTC GGAACCCAAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ACGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCCATC AGAACCTGGG 
CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGGAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATG GTAGCAACAG GGGGGAAGCT GCAGGCCCTA GTGGAGAGCC ACAGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitsugumin 53 promotes mitochondrial autophagy through regulating Ambra1 expression in C2C12 myoblast cells.
Cell biology international43(3)290-298(2019 Mar)
Lijie G,Yueyue Z,Nan Z,Ling W,Xuan W,Weijie Y

GIT1 contributes to autophagy in osteoclast through disruption of the binding of Beclin1 and Bcl2 under starvation condition.
Cell death & disease9(12)1195(2018 12)
Zhao SJ,Kong FQ,Cai W,Xu T,Zhou ZM,Wang ZB,Xu AD,Yang YQ,Chen J,Tang PY,Wang Q,Cheng L,Luo YJ,Zhou Z,Li LW,Huang YF,Zhao X,Yin GY,Xue MX,Fan J

Ambra1 Shapes Hippocampal Inhibition/Excitation Balance: Role in Neurodevelopmental Disorders.
Molecular neurobiology55(10)7921-7940(2018 Oct)
Nobili A,Krashia P,Cordella A,La Barbera L,Dell'Acqua MC,Caruso A,Pignataro A,Marino R,Sciarra F,Biamonte F,Scattoni ML,Ammassari-Teule M,Cecconi F,Berretta N,Keller F,Mercuri NB,D'Amelio M

Microglia ablation alleviates myelin-associated catatonic signs in mice.
The Journal of clinical investigation128(2)734-745(2018 02)
Janova H,Arinrad S,Balmuth E,Mitjans M,Hertel J,Habes M,Bittner RA,Pan H,Goebbels S,Begemann M,Gerwig UC,Langner S,Werner HB,Kittel-Schneider S,Homuth G,Davatzikos C,Völzke H,West BL,Reif A,Grabe HJ,Boretius S,Ehrenreich H,Nave KA

Effects of caloric restriction on neuropathic pain, peripheral nerve degeneration and inflammation in normometabolic and autophagy defective prediabetic Ambra1 mice.
PloS one13(12)e0208596(2018)
Coccurello R,Nazio F,Rossi C,De Angelis F,Vacca V,Giacovazzo G,Procacci P,Magnaghi V,Ciavardelli D,Marinelli S

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Ambra1 cDNA ORF clone, Mus musculus(house mouse)

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