ambra1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ambra1a
Entrez Gene ID	100332642
Full Name	autophagy/beclin-1 regulator 1a
Synonyms	ambra1
General protein information	Preferred Names autophagy/beclin-1 regulator 1a Names activating molecule in BECN1-regulated autophagy protein 1 activating molecule in beclin-1-regulated autophagy autophagy/beclin-1 regulator 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001281992.1	NP_001268921.1	activating molecule in BECN1-regulated autophagy protein 1
XM_002667669.5	XP_002667715.3	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_002667669.6	XP_002667715.3	activating molecule in BECN1-regulated autophagy protein 1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ambra1	NP_766257.3
Gallus gallus (chicken)	AMBRA1	XP_004941659.1
Danio rerio (zebrafish)	ambra1	XP_002667715.3
Canis lupus familiaris (dog)	AMBRA1	XP_005631347.1
Rattus norvegicus (Norway rat)	Ambra1	NP_001127813.1
Xenopus tropicalis (tropical clawed frog)	ambra1	XP_004913444.1
Homo sapiens (human)	AMBRA1	NP_060219.2
Pan troglodytes (chimpanzee)	AMBRA1	XP_508396.2
Macaca mulatta (Rhesus monkey)	AMBRA1	XP_001112256.1
Bos taurus (cattle)	AMBRA1	NP_001029694.2

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of the Apelin/Elabela Receptors (APLNR) in Chickens, Turtles, and Zebrafish: Identification of a Novel Apelin-Specific Receptor in Teleosts.
Zhang J, Zhou Y, Wu C, Wan Y, Fang C, Li J, Fang W, Yi R, Zhu G, Li J, Wang Y
Frontiers in endocrinology9756(2018)

Effects of diisononyl phthalate on Danio rerio reproduction.
Santangeli S, Maradonna F, Zanardini M, Notarstefano V, Gioacchini G, Forner-Piquer I, Habibi H, Carnevali O
Environmental pollution (Barking, Essex : 1987)231(Pt 1)1051-1062(2017 Dec)

BPA-Induced Deregulation Of Epigenetic Patterns: Effects On Female Zebrafish Reproduction.
Santangeli S, Maradonna F, Gioacchini G, Cobellis G, Piccinetti CC, Dalla Valle L, Carnevali O
Scientific reports621982(2016 Feb)

Characterization of Ambra1 in asexual cycle of a non-vertebrate chordate, the colonial tunicate Botryllus schlosseri, and phylogenetic analysis of the protein group in Bilateria.
Gasparini F, Skobo T, Benato F, Gioacchini G, Voskoboynik A, Carnevali O, Manni L, Dalla Valle L
Molecular phylogenetics and evolution9546-57(2016 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ambra1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ambra1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35863	NM_001281992.1 Latest version!	Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa13301	XM_002667669.6 Latest version!	Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
ODa13301	XM_002667669.5	Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ambra1a ( NM_001281992.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001268921.1 Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), mRNA.

Price & Availability↑

CloneID	ODa35863
Clone ID Related Accession (Same CDS sequence)	NM_001281992.1
Accession Version	NM_001281992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1561737600000
Organism	Danio rerio(zebrafish)
Product	activating molecule in BECN1-regulated autophagy protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from HE602022.1. On Aug 31, 2013 this sequence version replaced XM_005166436.1. ##Evidence-Data-START## Transcript exon combination :: HE602022.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAGCTGG GACAGAGGAA CTCAGTATGT ATCCTGTCCA GTCGAGAGCG GGGAGCACCG 
GGTCTTGCAT CATATAGGGT TCTGCAGCAG CTTGTAGAAG AGAAGACACA GCGAATGAAA 
TGGCAAAGTC AGAAAGTAGA GTTACCAGAC AGTCCCCGCT CAACCTTTCT GCTAGCCTTT 
AGTCCGGACA GGTCTTTAAT GGCTTCCACA CATGTGAACC ATAACATCTA CATCACTGAG 
GTTAAATCAG GAAAGTGTGT TCATTCATTG GTGGGCCATC GCAGGACACC CTGGTGTCTA 
ACTTTTCACC CTATCATCCC TGGGCTTATT GCTTCTGGCT GCCTGGATGG AGAGGTCAGA 
ATCTGGGACC TACATGGTGG CAGTGAAAGC TGGCTTACAG AGAGTAACTC CGCAATCGCA 
TCTCTTGCTT TCCACCCCAC GGCTCAGCTG CTGCTCATTG CCACCAACAA TGAGGTGCAT 
CTGTGGGACT GGAGTCGGAA AGAGCCCTTC ACTGTGGTCA AAACTGCCAG TGAGACAGAG 
AGAGTCAGAT TGGTGAGATT TGATCCATTG GGTCACTATC TTCTAACTGC CATTGTAAAC 
CCTTCGAACC AACCGAATGA TGATGATCCT GAGATTCCAA TGGACAGTGT GGAGATGCCT 
CATCTCCGAC AGCGCTCCTT CCTGCAGTCC CAGCCTGCGA GGCGCACACC CATCTTGCAC 
AATTTCTTGC ACATCCTCAC TTCCCGCAAC TCTGTTCCTC AGGCTGGAGG TGCCCATTCT 
GCTTCCACTG ATGGGTCCTC CGATTCTTCT GGCCCTTACA CTCTTATGTG CGTTCAGCCG 
CTTGGAATGG TGTGTTTTTG TAGCCGTTGC TCTGCAGCAC GAGTTCCATC TCCACCTGAT 
GAGGACCCTT CAGACTCGGC ATCCCTGGAG GCACAAGCTC ACACCTTTTC TTCTGCACGC 
ACAGAGCCAC TGCAGATGTC ACGGTTTTCT GTTGAGTCTC GTGCTGCAAA TCGCTCCTCT 
GCATTCAGCA GCGTGTATGG TGGAGGCAGC AACATGCGGA ATCATTCTTC CAGCTCTGGC 
AGGAGGGGAG TGACTGGAAT GGCACCTGTC CCGCATTTCC GACAACATCC ACCAGGTAGG 
GAAGGTGGAG GACGTCACCC TGGAGCAGAC TGGACAGTCA GTGGACTTAA TGGACAAAGC 
AGTAGTATGA CTCCACAAAG AACTGGCGCT TCCTCTGTTA GCCTTCTTTC TGTCCTGCGA 
CAGCAAGAGA CTTCCTTTCA ATCACCTGTT TACACTTCTG CTAGTGATAG ATGGGGCAGC 
ACACCGGGAA CTAGTTCCAG CAGGCACCGT CCTCCAGAAG AAGAGGGACA GAGCAGCTCC 
TCTTCCATCC ACAGTGTCCT GCGTTGTAAC CTTTACCGCT ATTTTATGGA TTATGAGGGA 
ACGCAGGACA CGGTCCAGCC TCTTGATGGC AGCAGACAGG ACCAGCAGAC TCAGGAAATG 
CTCAACAACA ACATGGACCC AGAGCAACCA GGACCATCTC ATTACCAGTC ACCCTATAGC 
GGTGAAAACC CTCCCCACAG TCACATGAAC CGCTGTAGAG TATGCCACAA TCTTTTCACC 
TACAATCAGG GTAGCCGGCG CTGGGACAGA ACAGGTCAGC CTTCATCTAC TGAGAGAAAC 
ACACCATGGC AACCTTCTAG TTCTGCCTTC CACAGCGTAG CTCCAGTTTC ACAATCTAAT 
GAACACCTAC TGGAACACAG ACCCATAGAG TCCACACCAA ATACCCCAGA GCCTCATGTA 
CCATTTTCCC AGAGAACTGA CACTGGTCAG CATGAGGAGC AGGCCGTTGG GCTTGTCTTT 
AACCAGGAAA CTGGCCAACT GGAGCGTGTT TACAGACAGT CTGCCTCCTC TCGCTCTGCA 
AACATATCAC AAGGAGCCTT AAACCAGGAA ATGCCTGAAG ACACACCAGA TAACGATTAC 
TTGCGCAGGT TGTCCCCTGC AGCATATTAT GCACAGCGTA TGATCCAGTA TCTGTCTCGA 
CGGGACAGCG TTCGGCAGCA CTCCCACCGT CCCCCTAGCA GACCTCGACC CCTGTCCTCT 
AATCCCAGCA GTCTTTCCCC GAGCCCTGTC CCTAACGCAG AGAGCAGCGA AGTTGACTTT 
GAGGAGTTTG AGGAAAATGG AAGCAGATAT CGGACTCCTC GTAATGCTCG CATGTCTGCC 
CCCTCTCTTG GTCGTTTTGT TGGAACCAGG CGCTTTCTCT TGCCGGAGTT TTTGCCGTAT 
GCTGGAATCT TCCATGAGCG AGGGCAGCCA GGCCTGGCAA CGCACTCATC CGTCAATAGG 
GTCCTCGCAG GTGCTGTAAT TGGAGACGGT CAATCTGCAG TGGCCAGCAA CATCGCTAAC 
ACTACCTATC GTCTGCAGTG GTGGGACTTC ACCAAATTTG ACTTGCCTGA AATCAGTAAT 
GCGTCGGTGA ACGTTCTGGT GCCTAACTGT AAGATCTACA ACGATGCGAG CTGTGATATC 
TCAGCGGATG GGCAGTTGCT GGCCGTATTC ATTCCCAGCA GTCAGAGGGG TTTCCCTGAT 
GAGGGCATTC TGGCTGTTTA CTCTCTCGCT CCGCACAACC TGGGAGAGAT GCTCTACTCC 
AAAAGATTCG GTCCAAATGC CATCTCTGTC AGTTTGTCTC CGATGGGTCG TTACGTGATG 
GTGGGTCTGG CGTCTCGCCG CATCTTGCTG CATCAGATCA GCGATCACAT GGTGGCACAG 
GTCTTCAGAC TGCAGCAGCC TCACGCAGGA GAGACGTCCA TGAGGAGGGT GTTTGATGTG 
GTGTACCCAA TGGCACCAGA CCAGAGGAGA CACGTCAGTA TAAACTCTGC CCGCTGGCTG 
CCAGATCCGG GGCTTGGGCT CGCTTATGGG ACCAACAAAG GAGATCTTGT CATCTGCAGA 
CCTGTCGATG TGCATTCTGA TGGCAGCAGC ACCTCTGAAC ACTCTGAGCG AATGTTCACC 
ATCAATAATG GTGGAGGGGT TGGACCCAGT AGCAGCAGGA GTGGAGACAG GGCAGGGAGC 
AGCAGGACTG ATCGACGGTC ACGCAGAGAC ATAGGCCTGA TGAATGGAGT TGGTCTGCAG 
CCACAACCTC CCGCCGCTAG TGTTACATCA CAGGGGACGC AGACACAGAA CCAGCGACTA 
CAGCATGCAG AGACACAGAC CGACCGCGAT CTTCCAGATG ACCCCCAGCA GCCCAGCACT 
TCTCAGGGGT CACAGGTCAC TGATGCCACA GAATCTCTGG ATTTTGAGAC TCTGCCAGAG 
GATTCTGGAT CTGAGGTGGT ACCCGAGACA CCACCCCACA GCCGGCCTCA GGAAGACGAA 
GGGTCAGATC CCAGCGAACC CAGCACAGAC AGCACAGGTC AGGCTGAATA TGTTTCTCGT 
ATCCGAAGGT TAATGGCTGA GGGAGGCATG ACTGCGGTTG TCCAGCGTGA GCAGAGCACC 
ACAATGGCGT CCATGGGCAG CTTTGGCAAC AATATCATCG TCAGCCATCG CATCCACCGA 
GGCTCCCAGA CAGGAGCGGA TGCACAAAAT CGTACTCGTT TAAGTCCAAT ACCTGGTCCT 
TCTTCTGGTG CTCCGGAGTC TTTAGCTGCA GCTTCGTATT CGCGCGTCCT CACTAATACA 
TTAGGCTTTC GTGGCGACAC AGCTCAAGGC ATAGATCTCA CAGAACAGGA GAGACTGCAC 
ACATCCTTCT TTACACCGGA GTTCTCTCCT CTTTTCTCAT CTGCTGTCGA TGCAACCGGT 
CCTTCCTCCT CAATAGGAGC AGACAGCGTC CTGGAGGGAG AAGACTTTCA CGACTTTGCG 
TCTCTCCCTC CATCTTTACT CTCATCCTCG CCGTCTCTTT CACCAGTGAA TAACAGTAAC 
TACAGCAACA GCGATAGTAG TTATCTTGGA GATGAGTACG GGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268921.1
CDS	308..4255
Translation	MKLGQRNSVCILSSRERGAPGLASYRVLQQLVEEKTQRMKWQSQKVELPDSPRSTFLLAFSPDRSLMASTHVNHNIYITEVKSGKCVHSLVGHRRTPWCLTFHPIIPGLIASGCLDGEVRIWDLHGGSESWLTESNSAIASLAFHPTAQLLLIATNNEVHLWDWSRKEPFTVVKTASETERVRLVRFDPLGHYLLTAIVNPSNQPNDDDPEIPMDSVEMPHLRQRSFLQSQPARRTPILHNFLHILTSRNSVPQAGGAHSASTDGSSDSSGPYTLMCVQPLGMVCFCSRCSAARVPSPPDEDPSDSASLEAQAHTFSSARTEPLQMSRFSVESRAANRSSAFSSVYGGGSNMRNHSSSSGRRGVTGMAPVPHFRQHPPGREGGGRHPGADWTVSGLNGQSSSMTPQRTGASSVSLLSVLRQQETSFQSPVYTSASDRWGSTPGTSSSRHRPPEEEGQSSSSSIHSVLRCNLYRYFMDYEGTQDTVQPLDGSRQDQQTQEMLNNNMDPEQPGPSHYQSPYSGENPPHSHMNRCRVCHNLFTYNQGSRRWDRTGQPSSTERNTPWQPSSSAFHSVAPVSQSNEHLLEHRPIESTPNTPEPHVPFSQRTDTGQHEEQAVGLVFNQETGQLERVYRQSASSRSANISQGALNQEMPEDTPDNDYLRRLSPAAYYAQRMIQYLSRRDSVRQHSHRPPSRPRPLSSNPSSLSPSPVPNAESSEVDFEEFEENGSRYRTPRNARMSAPSLGRFVGTRRFLLPEFLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVPNCKIYNDASCDISADGQLLAVFIPSSQRGFPDEGILAVYSLAPHNLGEMLYSKRFGPNAISVSLSPMGRYVMVGLASRRILLHQISDHMVAQVFRLQQPHAGETSMRRVFDVVYPMAPDQRRHVSINSARWLPDPGLGLAYGTNKGDLVICRPVDVHSDGSSTSEHSERMFTINNGGGVGPSSSRSGDRAGSSRTDRRSRRDIGLMNGVGLQPQPPAASVTSQGTQTQNQRLQHAETQTDRDLPDDPQQPSTSQGSQVTDATESLDFETLPEDSGSEVVPETPPHSRPQEDEGSDPSEPSTDSTGQAEYVSRIRRLMAEGGMTAVVQREQSTTMASMGSFGNNIIVSHRIHRGSQTGADAQNRTRLSPIPGPSSGAPESLAAASYSRVLTNTLGFRGDTAQGIDLTEQERLHTSFFTPEFSPLFSSAVDATGPSSSIGADSVLEGEDFHDFASLPPSLLSSSPSLSPVNNSNYSNSDSSYLGDEYGR

Target ORF information:

RefSeq Version	NM_001281992.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281992.1

ORF Insert Sequence:

ATGAAGCTGG GACAGAGGAA CTCAGTATGT ATCCTGTCCA GTCGAGAGCG GGGAGCACCG 
GGTCTTGCAT CATATAGGGT TCTGCAGCAG CTTGTAGAAG AGAAGACACA GCGAATGAAA 
TGGCAAAGTC AGAAAGTAGA GTTACCAGAC AGTCCCCGCT CAACCTTTCT GCTAGCCTTT 
AGTCCGGACA GGTCTTTAAT GGCTTCCACA CATGTGAACC ATAACATCTA CATCACTGAG 
GTTAAATCAG GAAAGTGTGT TCATTCATTG GTGGGCCATC GCAGGACACC CTGGTGTCTA 
ACTTTTCACC CTATCATCCC TGGGCTTATT GCTTCTGGCT GCCTGGATGG AGAGGTCAGA 
ATCTGGGACC TACATGGTGG CAGTGAAAGC TGGCTTACAG AGAGTAACTC CGCAATCGCA 
TCTCTTGCTT TCCACCCCAC GGCTCAGCTG CTGCTCATTG CCACCAACAA TGAGGTGCAT 
CTGTGGGACT GGAGTCGGAA AGAGCCCTTC ACTGTGGTCA AAACTGCCAG TGAGACAGAG 
AGAGTCAGAT TGGTGAGATT TGATCCATTG GGTCACTATC TTCTAACTGC CATTGTAAAC 
CCTTCGAACC AACCGAATGA TGATGATCCT GAGATTCCAA TGGACAGTGT GGAGATGCCT 
CATCTCCGAC AGCGCTCCTT CCTGCAGTCC CAGCCTGCGA GGCGCACACC CATCTTGCAC 
AATTTCTTGC ACATCCTCAC TTCCCGCAAC TCTGTTCCTC AGGCTGGAGG TGCCCATTCT 
GCTTCCACTG ATGGGTCCTC CGATTCTTCT GGCCCTTACA CTCTTATGTG CGTTCAGCCG 
CTTGGAATGG TGTGTTTTTG TAGCCGTTGC TCTGCAGCAC GAGTTCCATC TCCACCTGAT 
GAGGACCCTT CAGACTCGGC ATCCCTGGAG GCACAAGCTC ACACCTTTTC TTCTGCACGC 
ACAGAGCCAC TGCAGATGTC ACGGTTTTCT GTTGAGTCTC GTGCTGCAAA TCGCTCCTCT 
GCATTCAGCA GCGTGTATGG TGGAGGCAGC AACATGCGGA ATCATTCTTC CAGCTCTGGC 
AGGAGGGGAG TGACTGGAAT GGCACCTGTC CCGCATTTCC GACAACATCC ACCAGGTAGG 
GAAGGTGGAG GACGTCACCC TGGAGCAGAC TGGACAGTCA GTGGACTTAA TGGACAAAGC 
AGTAGTATGA CTCCACAAAG AACTGGCGCT TCCTCTGTTA GCCTTCTTTC TGTCCTGCGA 
CAGCAAGAGA CTTCCTTTCA ATCACCTGTT TACACTTCTG CTAGTGATAG ATGGGGCAGC 
ACACCGGGAA CTAGTTCCAG CAGGCACCGT CCTCCAGAAG AAGAGGGACA GAGCAGCTCC 
TCTTCCATCC ACAGTGTCCT GCGTTGTAAC CTTTACCGCT ATTTTATGGA TTATGAGGGA 
ACGCAGGACA CGGTCCAGCC TCTTGATGGC AGCAGACAGG ACCAGCAGAC TCAGGAAATG 
CTCAACAACA ACATGGACCC AGAGCAACCA GGACCATCTC ATTACCAGTC ACCCTATAGC 
GGTGAAAACC CTCCCCACAG TCACATGAAC CGCTGTAGAG TATGCCACAA TCTTTTCACC 
TACAATCAGG GTAGCCGGCG CTGGGACAGA ACAGGTCAGC CTTCATCTAC TGAGAGAAAC 
ACACCATGGC AACCTTCTAG TTCTGCCTTC CACAGCGTAG CTCCAGTTTC ACAATCTAAT 
GAACACCTAC TGGAACACAG ACCCATAGAG TCCACACCAA ATACCCCAGA GCCTCATGTA 
CCATTTTCCC AGAGAACTGA CACTGGTCAG CATGAGGAGC AGGCCGTTGG GCTTGTCTTT 
AACCAGGAAA CTGGCCAACT GGAGCGTGTT TACAGACAGT CTGCCTCCTC TCGCTCTGCA 
AACATATCAC AAGGAGCCTT AAACCAGGAA ATGCCTGAAG ACACACCAGA TAACGATTAC 
TTGCGCAGGT TGTCCCCTGC AGCATATTAT GCACAGCGTA TGATCCAGTA TCTGTCTCGA 
CGGGACAGCG TTCGGCAGCA CTCCCACCGT CCCCCTAGCA GACCTCGACC CCTGTCCTCT 
AATCCCAGCA GTCTTTCCCC GAGCCCTGTC CCTAACGCAG AGAGCAGCGA AGTTGACTTT 
GAGGAGTTTG AGGAAAATGG AAGCAGATAT CGGACTCCTC GTAATGCTCG CATGTCTGCC 
CCCTCTCTTG GTCGTTTTGT TGGAACCAGG CGCTTTCTCT TGCCGGAGTT TTTGCCGTAT 
GCTGGAATCT TCCATGAGCG AGGGCAGCCA GGCCTGGCAA CGCACTCATC CGTCAATAGG 
GTCCTCGCAG GTGCTGTAAT TGGAGACGGT CAATCTGCAG TGGCCAGCAA CATCGCTAAC 
ACTACCTATC GTCTGCAGTG GTGGGACTTC ACCAAATTTG ACTTGCCTGA AATCAGTAAT 
GCGTCGGTGA ACGTTCTGGT GCCTAACTGT AAGATCTACA ACGATGCGAG CTGTGATATC 
TCAGCGGATG GGCAGTTGCT GGCCGTATTC ATTCCCAGCA GTCAGAGGGG TTTCCCTGAT 
GAGGGCATTC TGGCTGTTTA CTCTCTCGCT CCGCACAACC TGGGAGAGAT GCTCTACTCC 
AAAAGATTCG GTCCAAATGC CATCTCTGTC AGTTTGTCTC CGATGGGTCG TTACGTGATG 
GTGGGTCTGG CGTCTCGCCG CATCTTGCTG CATCAGATCA GCGATCACAT GGTGGCACAG 
GTCTTCAGAC TGCAGCAGCC TCACGCAGGA GAGACGTCCA TGAGGAGGGT GTTTGATGTG 
GTGTACCCAA TGGCACCAGA CCAGAGGAGA CACGTCAGTA TAAACTCTGC CCGCTGGCTG 
CCAGATCCGG GGCTTGGGCT CGCTTATGGG ACCAACAAAG GAGATCTTGT CATCTGCAGA 
CCTGTCGATG TGCATTCTGA TGGCAGCAGC ACCTCTGAAC ACTCTGAGCG AATGTTCACC 
ATCAATAATG GTGGAGGGGT TGGACCCAGT AGCAGCAGGA GTGGAGACAG GGCAGGGAGC 
AGCAGGACTG ATCGACGGTC ACGCAGAGAC ATAGGCCTGA TGAATGGAGT TGGTCTGCAG 
CCACAACCTC CCGCCGCTAG TGTTACATCA CAGGGGACGC AGACACAGAA CCAGCGACTA 
CAGCATGCAG AGACACAGAC CGACCGCGAT CTTCCAGATG ACCCCCAGCA GCCCAGCACT 
TCTCAGGGGT CACAGGTCAC TGATGCCACA GAATCTCTGG ATTTTGAGAC TCTGCCAGAG 
GATTCTGGAT CTGAGGTGGT ACCCGAGACA CCACCCCACA GCCGGCCTCA GGAAGACGAA 
GGGTCAGATC CCAGCGAACC CAGCACAGAC AGCACAGGTC AGGCTGAATA TGTTTCTCGT 
ATCCGAAGGT TAATGGCTGA GGGAGGCATG ACTGCGGTTG TCCAGCGTGA GCAGAGCACC 
ACAATGGCGT CCATGGGCAG CTTTGGCAAC AATATCATCG TCAGCCATCG CATCCACCGA 
GGCTCCCAGA CAGGAGCGGA TGCACAAAAT CGTACTCGTT TAAGTCCAAT ACCTGGTCCT 
TCTTCTGGTG CTCCGGAGTC TTTAGCTGCA GCTTCGTATT CGCGCGTCCT CACTAATACA 
TTAGGCTTTC GTGGCGACAC AGCTCAAGGC ATAGATCTCA CAGAACAGGA GAGACTGCAC 
ACATCCTTCT TTACACCGGA GTTCTCTCCT CTTTTCTCAT CTGCTGTCGA TGCAACCGGT 
CCTTCCTCCT CAATAGGAGC AGACAGCGTC CTGGAGGGAG AAGACTTTCA CGACTTTGCG 
TCTCTCCCTC CATCTTTACT CTCATCCTCG CCGTCTCTTT CACCAGTGAA TAACAGTAAC 
TACAGCAACA GCGATAGTAG TTATCTTGGA GATGAGTACG GGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ambra1a ( XM_002667669.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002667715.3 Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13301
Clone ID Related Accession (Same CDS sequence)	XM_002667669.5 , XM_002667669.6
Accession Version	XM_002667669.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002667669.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGG GACAGAGGAA CTCAGTATGT ATCCTGTCCA GTCGAGAGCG GGGAGCACCG 
GGTCTTGCAT CATATAGGGT TCTGCAGCAG CTTGTAGAAG AGAAGACACA GCGAATGAAA 
TGGCAAAGTC AGAAAGTAGA GTTACCAGAC AGTCCCCGCT CAACCTTTCT GCTAGCCTTT 
AGTCCGGACA GGTCTTTAAT GGCTTCCACA CATGTGAACC ATAACATCTA CATCACTGAG 
GTTAAATCAG GAAAGTGTGT TCATTCATTG GTGGGCCATC GCAGGACACC CTGGTGTCTA 
ACTTTTCACC CTATCATCCC TGGGCTTATT GCTTCTGGCT GCCTGGACGG AGAGGTCAGA 
ATCTGGGACC TACATGGTGG CAGTGAAAGC TGGCTTACAG AGAGTAACTC CGCAATCGCA 
TCTCTTGCTT TCCACCCCAC GGCTCAGCTG CTGCTCATTG CCACCAACAA TGAGGTGCAT 
CTGTGGGACT GGAGTCGGAA AGAGCCCTTC ACTGTGGTCA AAACTGCCAG TGAGACAGAG 
AGAGTCAGAT TGGTGAGATT TGATCCATTG GGTCACTATC TTCTAACTGC CATTGTAAAC 
CCTTCGAACC AACCGAATGA TGATGATCCT GAGATTCCAA TGGACAGTGT GGAGATGCCT 
CATCTCCGAC AGCGCTCCTT CCTGCAGTCC CAGCCTGCGA GGCGCACACC CATCTTGCAC 
AATTTCTTGC ACATCCTCAC TTCCCGCAAC TCTGTTCCTC AGGCTGGAGG TGCCCATTCT 
GCTTCCACTG ATGGGTCCTC CGATTCTTCT GGCCCTTACA CTCTTATGTG CGTTCAGCCG 
CTTGGAATGG TGTGTTTTTG TAGCCGTTGC TCTGCAGCAC GAGTTCCATC TCCACCTGAT 
GAGGACCCTT CAGACTCGGC ATCCCTGGAG GCACAAGCTC ACACCTTTTC TTCTGCACGC 
ACAGAGCCAC TGCAGATGTC ACGGTTTTCT GTTGAGTCTC GTGCTGCAAA TCGCTCCTCT 
GCATTCAGCA GCGTGTATGG TGGAGGCAGC AACATGCGGA ATCATTCTTC CAGCTCTGGC 
AGGAGGGGAG TGACTGGAAT GGCACCTGTC CCGCATTTCC GACAACATCC ACCAGGTAGG 
GAAGGTGGAG GACGTCACCC TGGAGCAGAC TGGACAGTCA GTGGACTTAA TGGACAAAGC 
AGTAGTATGA CTCCACAAAG AACTGGCGCT TCCTCTGTTA GCCTTCTTTC TGTCCTGCGA 
CAGCAAGAGA CTTCCTTTCA ATCACCTGTT TACACTTCTG CTAGTGATAG ATGGGGCAGC 
ACACCGGGAA CTAGTTCCAG CAGGCACCGT CCTCCAGAAG AAGAGGGACA GAGCAGCTCC 
TCTTCCATCC ACAGTGTCCT GCGTTGTAAC CTTTACCGCT ATTTTATGGA TTATGAGGGA 
ACGCAGGACA CGGTCCAGCC TCTTGATGGC AGCAGACAGG ACCAGCAGAC TCAGGAAATG 
CTCAACAACA ACATGGACCC AGAGCAACCA GGACCATCTC ATTACCAGTC ACCCTATAGC 
GGTGAAAACC CTCCCCACAG TCACATGAAC CGCTGTAGAG TATGCCACAA TCTTTTCACC 
TACAATCAGG GTAGCCGGCG CTGGGACAGA ACAGGTCAGC CTTCATCTAC TGAGAGAAAC 
ACACCATGGC AACCTTCTAG TTCTGCCTTC CACAGCGTAG CTCCAGTTTC ACAATCTAAT 
GAACACCTAC TGGAACACAG ACCCATAGAG TCCACACCAA ATACCCCAGA GCCTCATGTA 
CCATTTTCCC AGAGAACTGA CACTGGTCAG CATGAGGAGC AGGCCGTTGG GCTTGTCTTT 
AACCAGGAAA CTGGCCAACT GGAGCGTGTT TACAGACAGT CTGCCTCCTC TCGCTCTGCA 
AACATATCAC AAGGAGCCTT AAACCAGGAA ATGCCTGAAG ACACACCAGA TAACGATTAC 
TTGCGCAGGA GGCTGCTGGA GTCGTCCATG ATGTCTCTTT CGAGAAATGG CACCTCTGGG 
TCACGAGACC ACCCCATCTA TCCCGACCCA GCCAGGTTGT CCCCTGCAGC ATATTATGCA 
CAGCGTATGA TCCAGTATCT GTCTCGACGG GACAGCGTTC GGCAGCACTC CCACCGTCCC 
CCTAGCAGAC CTCGACCCCT GTCCTCTAAT CCCAGCAGTC TTTCCCCGAG CCCTGTCCCT 
AACGCAGAGA GCAGCGAAGT TGACTTTGAG GAGTTTGAGG AAAATGGAAG CAGATATCGG 
ACTCCTCGTA ATGCTCGCAT GTCTGCCCCC TCTCTTGGTC GTTTTGTTGG AACCAGGCGC 
TTTCTCTTGC CGGAGTTTTT GCCGTATGCT GGAATCTTCC ATGAGCGAGG GCAGCCAGGC 
CTGGCAACGC ACTCATCCGT CAATAGGGTC CTCGCAGGTG CTGTAATTGG AGACGGTCAA 
TCTGCAGTGG CCAGCAACAT CGCTAACACT ACCTATCGTC TGCAGTGGTG GGACTTCACC 
AAATTTGACT TGCCTGAAAT CAGTAATGCG TCGGTGAACG TTCTGGTGCC TAACTGTAAG 
ATCTACAACG ATGCGAGCTG TGATATCTCA GCGGATGGGC AGTTGCTGGC CGTATTCATT 
CCCAGCAGTC AGAGGGGTTT CCCTGATGAG GGCATTCTGG CTGTTTACTC TCTCGCTCCG 
CACAACCTGG GAGAGATGCT CTACTCCAAA AGATTCGGTC CAAATGCCAT CTCTGTCAGT 
TTGTCTCCGA TGGGTCGTTA CGTGATGGTG GGTCTGGCGT CTCGCCGCAT CCTGCTGCAT 
CAGATCAGCG ATCACATGGT GGCACAGGTC TTCAGACTGC AGCAGCCTCA CGCAGGAGAG 
ACGTCCATGA GGAGGGTGTT TGATGTGGTG TACCCAATGG CACCAGACCA GAGGAGACAC 
GTCAGTATAA ACTCTGCCCG CTGGCTGCCA GATCCGGGGC TTGGGCTCGC TTATGGGACC 
AACAAAGGAG ATCTTGTCAT CTGCAGACCT GTCGATGTGC ATTCTGATGG CAGCAGCACC 
TCTGAACACT CTGAGCGAAT GTTCACCATC AATAATGGTG GAGGGGTTGG ACCCAGTAGC 
AGCAGGAGTG GAGACAGGGC AGGGAGCAGC AGGACTGATC GACGGTCACG CAGAGACATA 
GGCCTGATGA ATGGAGTTGG TCTGCAGCCA CAACCTCCCG CCGCTAGTGT TACATCACAG 
GGGACGCAGA CACAGAACCA GCGACTACAG CATGCAGAGA CACAGACCGA CCGCGATCTT 
CCAGATGACC CCCAGCAGCC CAGCACTTCT CAGGGGTCAC AGGTCACTGA TGCCACAGAA 
TCTCTGGATT TTGAGACTCT GCCAGAGGAT TCTGGATCTG AGGTGGTACC CGAGACACCA 
CCCCACAGCC GGCCTCAGGA AGACGAAGGG TCAGATCCCA GCGAACCCAG CACAGACAGC 
ACAGGTCAGG CTGAATATGT TTCTCGTATC CGAAGGTTAA TGGCTGAGGG AGGCATGACT 
GCGGTTGTCC AGCGTGAGCA GAGCACCACA ATGGCGTCCA TGGGCAGCTT TGGCAACAAT 
ATCATCGTCA GCCATCGCAT CCACCGAGGC TCCCAGACAG GAGCGGATGC ACAAAATCGT 
ACTCGTTTAA GTCCAATACC TGGTCCTTCT TCTGGTGCTC CGGAGTCTTT AGCTGCAGCT 
TCGTATTCGC GCGTCCTCAC TAATACATTA GGCTTTCGTG GCGACACAGC TCAAGGCATA 
GATCTCACAG AACAGGAGAG ACTGCACACA TCCTTCTTTA CACCGGAGTT CTCTCCTCTT 
TTCTCATCTG CTGTCGATGC AACCGGTCCT TCCTCCTCAA TAGGAGCAGA CAGCGTCCTG 
GAGGGAGAAG ACTTTCACGA CTTTGCGTCT CTCCCTCCAT CTTTACTCTC ATCCTCGCCG 
TCTCTTTCAC CAGTGAATAA CAGTAACTAC AGCAACAGCG ATAGTAGTTA TCTTGGAGAT 
GAGTACGGGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002667715.3
CDS	385..4419
Translation	MKLGQRNSVCILSSRERGAPGLASYRVLQQLVEEKTQRMKWQSQKVELPDSPRSTFLLAFSPDRSLMASTHVNHNIYITEVKSGKCVHSLVGHRRTPWCLTFHPIIPGLIASGCLDGEVRIWDLHGGSESWLTESNSAIASLAFHPTAQLLLIATNNEVHLWDWSRKEPFTVVKTASETERVRLVRFDPLGHYLLTAIVNPSNQPNDDDPEIPMDSVEMPHLRQRSFLQSQPARRTPILHNFLHILTSRNSVPQAGGAHSASTDGSSDSSGPYTLMCVQPLGMVCFCSRCSAARVPSPPDEDPSDSASLEAQAHTFSSARTEPLQMSRFSVESRAANRSSAFSSVYGGGSNMRNHSSSSGRRGVTGMAPVPHFRQHPPGREGGGRHPGADWTVSGLNGQSSSMTPQRTGASSVSLLSVLRQQETSFQSPVYTSASDRWGSTPGTSSSRHRPPEEEGQSSSSSIHSVLRCNLYRYFMDYEGTQDTVQPLDGSRQDQQTQEMLNNNMDPEQPGPSHYQSPYSGENPPHSHMNRCRVCHNLFTYNQGSRRWDRTGQPSSTERNTPWQPSSSAFHSVAPVSQSNEHLLEHRPIESTPNTPEPHVPFSQRTDTGQHEEQAVGLVFNQETGQLERVYRQSASSRSANISQGALNQEMPEDTPDNDYLRRRLLESSMMSLSRNGTSGSRDHPIYPDPARLSPAAYYAQRMIQYLSRRDSVRQHSHRPPSRPRPLSSNPSSLSPSPVPNAESSEVDFEEFEENGSRYRTPRNARMSAPSLGRFVGTRRFLLPEFLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVPNCKIYNDASCDISADGQLLAVFIPSSQRGFPDEGILAVYSLAPHNLGEMLYSKRFGPNAISVSLSPMGRYVMVGLASRRILLHQISDHMVAQVFRLQQPHAGETSMRRVFDVVYPMAPDQRRHVSINSARWLPDPGLGLAYGTNKGDLVICRPVDVHSDGSSTSEHSERMFTINNGGGVGPSSSRSGDRAGSSRTDRRSRRDIGLMNGVGLQPQPPAASVTSQGTQTQNQRLQHAETQTDRDLPDDPQQPSTSQGSQVTDATESLDFETLPEDSGSEVVPETPPHSRPQEDEGSDPSEPSTDSTGQAEYVSRIRRLMAEGGMTAVVQREQSTTMASMGSFGNNIIVSHRIHRGSQTGADAQNRTRLSPIPGPSSGAPESLAAASYSRVLTNTLGFRGDTAQGIDLTEQERLHTSFFTPEFSPLFSSAVDATGPSSSIGADSVLEGEDFHDFASLPPSLLSSSPSLSPVNNSNYSNSDSSYLGDEYGR

Target ORF information:

RefSeq Version	XM_002667669.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002667669.5

ORF Insert Sequence:

ATGAAGCTGG GACAGAGGAA CTCAGTATGT ATCCTGTCCA GTCGAGAGCG GGGAGCACCG 
GGTCTTGCAT CATATAGGGT TCTGCAGCAG CTTGTAGAAG AGAAGACACA GCGAATGAAA 
TGGCAAAGTC AGAAAGTAGA GTTACCAGAC AGTCCCCGCT CAACCTTTCT GCTAGCCTTT 
AGTCCGGACA GGTCTTTAAT GGCTTCCACA CATGTGAACC ATAACATCTA CATCACTGAG 
GTTAAATCAG GAAAGTGTGT TCATTCATTG GTGGGCCATC GCAGGACACC CTGGTGTCTA 
ACTTTTCACC CTATCATCCC TGGGCTTATT GCTTCTGGCT GCCTGGACGG AGAGGTCAGA 
ATCTGGGACC TACATGGTGG CAGTGAAAGC TGGCTTACAG AGAGTAACTC CGCAATCGCA 
TCTCTTGCTT TCCACCCCAC GGCTCAGCTG CTGCTCATTG CCACCAACAA TGAGGTGCAT 
CTGTGGGACT GGAGTCGGAA AGAGCCCTTC ACTGTGGTCA AAACTGCCAG TGAGACAGAG 
AGAGTCAGAT TGGTGAGATT TGATCCATTG GGTCACTATC TTCTAACTGC CATTGTAAAC 
CCTTCGAACC AACCGAATGA TGATGATCCT GAGATTCCAA TGGACAGTGT GGAGATGCCT 
CATCTCCGAC AGCGCTCCTT CCTGCAGTCC CAGCCTGCGA GGCGCACACC CATCTTGCAC 
AATTTCTTGC ACATCCTCAC TTCCCGCAAC TCTGTTCCTC AGGCTGGAGG TGCCCATTCT 
GCTTCCACTG ATGGGTCCTC CGATTCTTCT GGCCCTTACA CTCTTATGTG CGTTCAGCCG 
CTTGGAATGG TGTGTTTTTG TAGCCGTTGC TCTGCAGCAC GAGTTCCATC TCCACCTGAT 
GAGGACCCTT CAGACTCGGC ATCCCTGGAG GCACAAGCTC ACACCTTTTC TTCTGCACGC 
ACAGAGCCAC TGCAGATGTC ACGGTTTTCT GTTGAGTCTC GTGCTGCAAA TCGCTCCTCT 
GCATTCAGCA GCGTGTATGG TGGAGGCAGC AACATGCGGA ATCATTCTTC CAGCTCTGGC 
AGGAGGGGAG TGACTGGAAT GGCACCTGTC CCGCATTTCC GACAACATCC ACCAGGTAGG 
GAAGGTGGAG GACGTCACCC TGGAGCAGAC TGGACAGTCA GTGGACTTAA TGGACAAAGC 
AGTAGTATGA CTCCACAAAG AACTGGCGCT TCCTCTGTTA GCCTTCTTTC TGTCCTGCGA 
CAGCAAGAGA CTTCCTTTCA ATCACCTGTT TACACTTCTG CTAGTGATAG ATGGGGCAGC 
ACACCGGGAA CTAGTTCCAG CAGGCACCGT CCTCCAGAAG AAGAGGGACA GAGCAGCTCC 
TCTTCCATCC ACAGTGTCCT GCGTTGTAAC CTTTACCGCT ATTTTATGGA TTATGAGGGA 
ACGCAGGACA CGGTCCAGCC TCTTGATGGC AGCAGACAGG ACCAGCAGAC TCAGGAAATG 
CTCAACAACA ACATGGACCC AGAGCAACCA GGACCATCTC ATTACCAGTC ACCCTATAGC 
GGTGAAAACC CTCCCCACAG TCACATGAAC CGCTGTAGAG TATGCCACAA TCTTTTCACC 
TACAATCAGG GTAGCCGGCG CTGGGACAGA ACAGGTCAGC CTTCATCTAC TGAGAGAAAC 
ACACCATGGC AACCTTCTAG TTCTGCCTTC CACAGCGTAG CTCCAGTTTC ACAATCTAAT 
GAACACCTAC TGGAACACAG ACCCATAGAG TCCACACCAA ATACCCCAGA GCCTCATGTA 
CCATTTTCCC AGAGAACTGA CACTGGTCAG CATGAGGAGC AGGCCGTTGG GCTTGTCTTT 
AACCAGGAAA CTGGCCAACT GGAGCGTGTT TACAGACAGT CTGCCTCCTC TCGCTCTGCA 
AACATATCAC AAGGAGCCTT AAACCAGGAA ATGCCTGAAG ACACACCAGA TAACGATTAC 
TTGCGCAGGA GGCTGCTGGA GTCGTCCATG ATGTCTCTTT CGAGAAATGG CACCTCTGGG 
TCACGAGACC ACCCCATCTA TCCCGACCCA GCCAGGTTGT CCCCTGCAGC ATATTATGCA 
CAGCGTATGA TCCAGTATCT GTCTCGACGG GACAGCGTTC GGCAGCACTC CCACCGTCCC 
CCTAGCAGAC CTCGACCCCT GTCCTCTAAT CCCAGCAGTC TTTCCCCGAG CCCTGTCCCT 
AACGCAGAGA GCAGCGAAGT TGACTTTGAG GAGTTTGAGG AAAATGGAAG CAGATATCGG 
ACTCCTCGTA ATGCTCGCAT GTCTGCCCCC TCTCTTGGTC GTTTTGTTGG AACCAGGCGC 
TTTCTCTTGC CGGAGTTTTT GCCGTATGCT GGAATCTTCC ATGAGCGAGG GCAGCCAGGC 
CTGGCAACGC ACTCATCCGT CAATAGGGTC CTCGCAGGTG CTGTAATTGG AGACGGTCAA 
TCTGCAGTGG CCAGCAACAT CGCTAACACT ACCTATCGTC TGCAGTGGTG GGACTTCACC 
AAATTTGACT TGCCTGAAAT CAGTAATGCG TCGGTGAACG TTCTGGTGCC TAACTGTAAG 
ATCTACAACG ATGCGAGCTG TGATATCTCA GCGGATGGGC AGTTGCTGGC CGTATTCATT 
CCCAGCAGTC AGAGGGGTTT CCCTGATGAG GGCATTCTGG CTGTTTACTC TCTCGCTCCG 
CACAACCTGG GAGAGATGCT CTACTCCAAA AGATTCGGTC CAAATGCCAT CTCTGTCAGT 
TTGTCTCCGA TGGGTCGTTA CGTGATGGTG GGTCTGGCGT CTCGCCGCAT CCTGCTGCAT 
CAGATCAGCG ATCACATGGT GGCACAGGTC TTCAGACTGC AGCAGCCTCA CGCAGGAGAG 
ACGTCCATGA GGAGGGTGTT TGATGTGGTG TACCCAATGG CACCAGACCA GAGGAGACAC 
GTCAGTATAA ACTCTGCCCG CTGGCTGCCA GATCCGGGGC TTGGGCTCGC TTATGGGACC 
AACAAAGGAG ATCTTGTCAT CTGCAGACCT GTCGATGTGC ATTCTGATGG CAGCAGCACC 
TCTGAACACT CTGAGCGAAT GTTCACCATC AATAATGGTG GAGGGGTTGG ACCCAGTAGC 
AGCAGGAGTG GAGACAGGGC AGGGAGCAGC AGGACTGATC GACGGTCACG CAGAGACATA 
GGCCTGATGA ATGGAGTTGG TCTGCAGCCA CAACCTCCCG CCGCTAGTGT TACATCACAG 
GGGACGCAGA CACAGAACCA GCGACTACAG CATGCAGAGA CACAGACCGA CCGCGATCTT 
CCAGATGACC CCCAGCAGCC CAGCACTTCT CAGGGGTCAC AGGTCACTGA TGCCACAGAA 
TCTCTGGATT TTGAGACTCT GCCAGAGGAT TCTGGATCTG AGGTGGTACC CGAGACACCA 
CCCCACAGCC GGCCTCAGGA AGACGAAGGG TCAGATCCCA GCGAACCCAG CACAGACAGC 
ACAGGTCAGG CTGAATATGT TTCTCGTATC CGAAGGTTAA TGGCTGAGGG AGGCATGACT 
GCGGTTGTCC AGCGTGAGCA GAGCACCACA ATGGCGTCCA TGGGCAGCTT TGGCAACAAT 
ATCATCGTCA GCCATCGCAT CCACCGAGGC TCCCAGACAG GAGCGGATGC ACAAAATCGT 
ACTCGTTTAA GTCCAATACC TGGTCCTTCT TCTGGTGCTC CGGAGTCTTT AGCTGCAGCT 
TCGTATTCGC GCGTCCTCAC TAATACATTA GGCTTTCGTG GCGACACAGC TCAAGGCATA 
GATCTCACAG AACAGGAGAG ACTGCACACA TCCTTCTTTA CACCGGAGTT CTCTCCTCTT 
TTCTCATCTG CTGTCGATGC AACCGGTCCT TCCTCCTCAA TAGGAGCAGA CAGCGTCCTG 
GAGGGAGAAG ACTTTCACGA CTTTGCGTCT CTCCCTCCAT CTTTACTCTC ATCCTCGCCG 
TCTCTTTCAC CAGTGAATAA CAGTAACTAC AGCAACAGCG ATAGTAGTTA TCTTGGAGAT 
GAGTACGGGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ambra1a ( XM_002667669.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002667715.3 Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), transcript variant X1, mRNA.

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CloneID	ODa13301
Clone ID Related Accession (Same CDS sequence)	XM_002667669.5 , XM_002667669.6
Accession Version	XM_002667669.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002667669.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGG GACAGAGGAA CTCAGTATGT ATCCTGTCCA GTCGAGAGCG GGGAGCACCG 
GGTCTTGCAT CATATAGGGT TCTGCAGCAG CTTGTAGAAG AGAAGACACA GCGAATGAAA 
TGGCAAAGTC AGAAAGTAGA GTTACCAGAC AGTCCCCGCT CAACCTTTCT GCTAGCCTTT 
AGTCCGGACA GGTCTTTAAT GGCTTCCACA CATGTGAACC ATAACATCTA CATCACTGAG 
GTTAAATCAG GAAAGTGTGT TCATTCATTG GTGGGCCATC GCAGGACACC CTGGTGTCTA 
ACTTTTCACC CTATCATCCC TGGGCTTATT GCTTCTGGCT GCCTGGACGG AGAGGTCAGA 
ATCTGGGACC TACATGGTGG CAGTGAAAGC TGGCTTACAG AGAGTAACTC CGCAATCGCA 
TCTCTTGCTT TCCACCCCAC GGCTCAGCTG CTGCTCATTG CCACCAACAA TGAGGTGCAT 
CTGTGGGACT GGAGTCGGAA AGAGCCCTTC ACTGTGGTCA AAACTGCCAG TGAGACAGAG 
AGAGTCAGAT TGGTGAGATT TGATCCATTG GGTCACTATC TTCTAACTGC CATTGTAAAC 
CCTTCGAACC AACCGAATGA TGATGATCCT GAGATTCCAA TGGACAGTGT GGAGATGCCT 
CATCTCCGAC AGCGCTCCTT CCTGCAGTCC CAGCCTGCGA GGCGCACACC CATCTTGCAC 
AATTTCTTGC ACATCCTCAC TTCCCGCAAC TCTGTTCCTC AGGCTGGAGG TGCCCATTCT 
GCTTCCACTG ATGGGTCCTC CGATTCTTCT GGCCCTTACA CTCTTATGTG CGTTCAGCCG 
CTTGGAATGG TGTGTTTTTG TAGCCGTTGC TCTGCAGCAC GAGTTCCATC TCCACCTGAT 
GAGGACCCTT CAGACTCGGC ATCCCTGGAG GCACAAGCTC ACACCTTTTC TTCTGCACGC 
ACAGAGCCAC TGCAGATGTC ACGGTTTTCT GTTGAGTCTC GTGCTGCAAA TCGCTCCTCT 
GCATTCAGCA GCGTGTATGG TGGAGGCAGC AACATGCGGA ATCATTCTTC CAGCTCTGGC 
AGGAGGGGAG TGACTGGAAT GGCACCTGTC CCGCATTTCC GACAACATCC ACCAGGTAGG 
GAAGGTGGAG GACGTCACCC TGGAGCAGAC TGGACAGTCA GTGGACTTAA TGGACAAAGC 
AGTAGTATGA CTCCACAAAG AACTGGCGCT TCCTCTGTTA GCCTTCTTTC TGTCCTGCGA 
CAGCAAGAGA CTTCCTTTCA ATCACCTGTT TACACTTCTG CTAGTGATAG ATGGGGCAGC 
ACACCGGGAA CTAGTTCCAG CAGGCACCGT CCTCCAGAAG AAGAGGGACA GAGCAGCTCC 
TCTTCCATCC ACAGTGTCCT GCGTTGTAAC CTTTACCGCT ATTTTATGGA TTATGAGGGA 
ACGCAGGACA CGGTCCAGCC TCTTGATGGC AGCAGACAGG ACCAGCAGAC TCAGGAAATG 
CTCAACAACA ACATGGACCC AGAGCAACCA GGACCATCTC ATTACCAGTC ACCCTATAGC 
GGTGAAAACC CTCCCCACAG TCACATGAAC CGCTGTAGAG TATGCCACAA TCTTTTCACC 
TACAATCAGG GTAGCCGGCG CTGGGACAGA ACAGGTCAGC CTTCATCTAC TGAGAGAAAC 
ACACCATGGC AACCTTCTAG TTCTGCCTTC CACAGCGTAG CTCCAGTTTC ACAATCTAAT 
GAACACCTAC TGGAACACAG ACCCATAGAG TCCACACCAA ATACCCCAGA GCCTCATGTA 
CCATTTTCCC AGAGAACTGA CACTGGTCAG CATGAGGAGC AGGCCGTTGG GCTTGTCTTT 
AACCAGGAAA CTGGCCAACT GGAGCGTGTT TACAGACAGT CTGCCTCCTC TCGCTCTGCA 
AACATATCAC AAGGAGCCTT AAACCAGGAA ATGCCTGAAG ACACACCAGA TAACGATTAC 
TTGCGCAGGA GGCTGCTGGA GTCGTCCATG ATGTCTCTTT CGAGAAATGG CACCTCTGGG 
TCACGAGACC ACCCCATCTA TCCCGACCCA GCCAGGTTGT CCCCTGCAGC ATATTATGCA 
CAGCGTATGA TCCAGTATCT GTCTCGACGG GACAGCGTTC GGCAGCACTC CCACCGTCCC 
CCTAGCAGAC CTCGACCCCT GTCCTCTAAT CCCAGCAGTC TTTCCCCGAG CCCTGTCCCT 
AACGCAGAGA GCAGCGAAGT TGACTTTGAG GAGTTTGAGG AAAATGGAAG CAGATATCGG 
ACTCCTCGTA ATGCTCGCAT GTCTGCCCCC TCTCTTGGTC GTTTTGTTGG AACCAGGCGC 
TTTCTCTTGC CGGAGTTTTT GCCGTATGCT GGAATCTTCC ATGAGCGAGG GCAGCCAGGC 
CTGGCAACGC ACTCATCCGT CAATAGGGTC CTCGCAGGTG CTGTAATTGG AGACGGTCAA 
TCTGCAGTGG CCAGCAACAT CGCTAACACT ACCTATCGTC TGCAGTGGTG GGACTTCACC 
AAATTTGACT TGCCTGAAAT CAGTAATGCG TCGGTGAACG TTCTGGTGCC TAACTGTAAG 
ATCTACAACG ATGCGAGCTG TGATATCTCA GCGGATGGGC AGTTGCTGGC CGTATTCATT 
CCCAGCAGTC AGAGGGGTTT CCCTGATGAG GGCATTCTGG CTGTTTACTC TCTCGCTCCG 
CACAACCTGG GAGAGATGCT CTACTCCAAA AGATTCGGTC CAAATGCCAT CTCTGTCAGT 
TTGTCTCCGA TGGGTCGTTA CGTGATGGTG GGTCTGGCGT CTCGCCGCAT CCTGCTGCAT 
CAGATCAGCG ATCACATGGT GGCACAGGTC TTCAGACTGC AGCAGCCTCA CGCAGGAGAG 
ACGTCCATGA GGAGGGTGTT TGATGTGGTG TACCCAATGG CACCAGACCA GAGGAGACAC 
GTCAGTATAA ACTCTGCCCG CTGGCTGCCA GATCCGGGGC TTGGGCTCGC TTATGGGACC 
AACAAAGGAG ATCTTGTCAT CTGCAGACCT GTCGATGTGC ATTCTGATGG CAGCAGCACC 
TCTGAACACT CTGAGCGAAT GTTCACCATC AATAATGGTG GAGGGGTTGG ACCCAGTAGC 
AGCAGGAGTG GAGACAGGGC AGGGAGCAGC AGGACTGATC GACGGTCACG CAGAGACATA 
GGCCTGATGA ATGGAGTTGG TCTGCAGCCA CAACCTCCCG CCGCTAGTGT TACATCACAG 
GGGACGCAGA CACAGAACCA GCGACTACAG CATGCAGAGA CACAGACCGA CCGCGATCTT 
CCAGATGACC CCCAGCAGCC CAGCACTTCT CAGGGGTCAC AGGTCACTGA TGCCACAGAA 
TCTCTGGATT TTGAGACTCT GCCAGAGGAT TCTGGATCTG AGGTGGTACC CGAGACACCA 
CCCCACAGCC GGCCTCAGGA AGACGAAGGG TCAGATCCCA GCGAACCCAG CACAGACAGC 
ACAGGTCAGG CTGAATATGT TTCTCGTATC CGAAGGTTAA TGGCTGAGGG AGGCATGACT 
GCGGTTGTCC AGCGTGAGCA GAGCACCACA ATGGCGTCCA TGGGCAGCTT TGGCAACAAT 
ATCATCGTCA GCCATCGCAT CCACCGAGGC TCCCAGACAG GAGCGGATGC ACAAAATCGT 
ACTCGTTTAA GTCCAATACC TGGTCCTTCT TCTGGTGCTC CGGAGTCTTT AGCTGCAGCT 
TCGTATTCGC GCGTCCTCAC TAATACATTA GGCTTTCGTG GCGACACAGC TCAAGGCATA 
GATCTCACAG AACAGGAGAG ACTGCACACA TCCTTCTTTA CACCGGAGTT CTCTCCTCTT 
TTCTCATCTG CTGTCGATGC AACCGGTCCT TCCTCCTCAA TAGGAGCAGA CAGCGTCCTG 
GAGGGAGAAG ACTTTCACGA CTTTGCGTCT CTCCCTCCAT CTTTACTCTC ATCCTCGCCG 
TCTCTTTCAC CAGTGAATAA CAGTAACTAC AGCAACAGCG ATAGTAGTTA TCTTGGAGAT 
GAGTACGGGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002667715.3
CDS	322..4356
Translation	MKLGQRNSVCILSSRERGAPGLASYRVLQQLVEEKTQRMKWQSQKVELPDSPRSTFLLAFSPDRSLMASTHVNHNIYITEVKSGKCVHSLVGHRRTPWCLTFHPIIPGLIASGCLDGEVRIWDLHGGSESWLTESNSAIASLAFHPTAQLLLIATNNEVHLWDWSRKEPFTVVKTASETERVRLVRFDPLGHYLLTAIVNPSNQPNDDDPEIPMDSVEMPHLRQRSFLQSQPARRTPILHNFLHILTSRNSVPQAGGAHSASTDGSSDSSGPYTLMCVQPLGMVCFCSRCSAARVPSPPDEDPSDSASLEAQAHTFSSARTEPLQMSRFSVESRAANRSSAFSSVYGGGSNMRNHSSSSGRRGVTGMAPVPHFRQHPPGREGGGRHPGADWTVSGLNGQSSSMTPQRTGASSVSLLSVLRQQETSFQSPVYTSASDRWGSTPGTSSSRHRPPEEEGQSSSSSIHSVLRCNLYRYFMDYEGTQDTVQPLDGSRQDQQTQEMLNNNMDPEQPGPSHYQSPYSGENPPHSHMNRCRVCHNLFTYNQGSRRWDRTGQPSSTERNTPWQPSSSAFHSVAPVSQSNEHLLEHRPIESTPNTPEPHVPFSQRTDTGQHEEQAVGLVFNQETGQLERVYRQSASSRSANISQGALNQEMPEDTPDNDYLRRRLLESSMMSLSRNGTSGSRDHPIYPDPARLSPAAYYAQRMIQYLSRRDSVRQHSHRPPSRPRPLSSNPSSLSPSPVPNAESSEVDFEEFEENGSRYRTPRNARMSAPSLGRFVGTRRFLLPEFLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVPNCKIYNDASCDISADGQLLAVFIPSSQRGFPDEGILAVYSLAPHNLGEMLYSKRFGPNAISVSLSPMGRYVMVGLASRRILLHQISDHMVAQVFRLQQPHAGETSMRRVFDVVYPMAPDQRRHVSINSARWLPDPGLGLAYGTNKGDLVICRPVDVHSDGSSTSEHSERMFTINNGGGVGPSSSRSGDRAGSSRTDRRSRRDIGLMNGVGLQPQPPAASVTSQGTQTQNQRLQHAETQTDRDLPDDPQQPSTSQGSQVTDATESLDFETLPEDSGSEVVPETPPHSRPQEDEGSDPSEPSTDSTGQAEYVSRIRRLMAEGGMTAVVQREQSTTMASMGSFGNNIIVSHRIHRGSQTGADAQNRTRLSPIPGPSSGAPESLAAASYSRVLTNTLGFRGDTAQGIDLTEQERLHTSFFTPEFSPLFSSAVDATGPSSSIGADSVLEGEDFHDFASLPPSLLSSSPSLSPVNNSNYSNSDSSYLGDEYGR

Target ORF information:

RefSeq Version	XM_002667669.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002667669.6

ORF Insert Sequence:

ATGAAGCTGG GACAGAGGAA CTCAGTATGT ATCCTGTCCA GTCGAGAGCG GGGAGCACCG 
GGTCTTGCAT CATATAGGGT TCTGCAGCAG CTTGTAGAAG AGAAGACACA GCGAATGAAA 
TGGCAAAGTC AGAAAGTAGA GTTACCAGAC AGTCCCCGCT CAACCTTTCT GCTAGCCTTT 
AGTCCGGACA GGTCTTTAAT GGCTTCCACA CATGTGAACC ATAACATCTA CATCACTGAG 
GTTAAATCAG GAAAGTGTGT TCATTCATTG GTGGGCCATC GCAGGACACC CTGGTGTCTA 
ACTTTTCACC CTATCATCCC TGGGCTTATT GCTTCTGGCT GCCTGGACGG AGAGGTCAGA 
ATCTGGGACC TACATGGTGG CAGTGAAAGC TGGCTTACAG AGAGTAACTC CGCAATCGCA 
TCTCTTGCTT TCCACCCCAC GGCTCAGCTG CTGCTCATTG CCACCAACAA TGAGGTGCAT 
CTGTGGGACT GGAGTCGGAA AGAGCCCTTC ACTGTGGTCA AAACTGCCAG TGAGACAGAG 
AGAGTCAGAT TGGTGAGATT TGATCCATTG GGTCACTATC TTCTAACTGC CATTGTAAAC 
CCTTCGAACC AACCGAATGA TGATGATCCT GAGATTCCAA TGGACAGTGT GGAGATGCCT 
CATCTCCGAC AGCGCTCCTT CCTGCAGTCC CAGCCTGCGA GGCGCACACC CATCTTGCAC 
AATTTCTTGC ACATCCTCAC TTCCCGCAAC TCTGTTCCTC AGGCTGGAGG TGCCCATTCT 
GCTTCCACTG ATGGGTCCTC CGATTCTTCT GGCCCTTACA CTCTTATGTG CGTTCAGCCG 
CTTGGAATGG TGTGTTTTTG TAGCCGTTGC TCTGCAGCAC GAGTTCCATC TCCACCTGAT 
GAGGACCCTT CAGACTCGGC ATCCCTGGAG GCACAAGCTC ACACCTTTTC TTCTGCACGC 
ACAGAGCCAC TGCAGATGTC ACGGTTTTCT GTTGAGTCTC GTGCTGCAAA TCGCTCCTCT 
GCATTCAGCA GCGTGTATGG TGGAGGCAGC AACATGCGGA ATCATTCTTC CAGCTCTGGC 
AGGAGGGGAG TGACTGGAAT GGCACCTGTC CCGCATTTCC GACAACATCC ACCAGGTAGG 
GAAGGTGGAG GACGTCACCC TGGAGCAGAC TGGACAGTCA GTGGACTTAA TGGACAAAGC 
AGTAGTATGA CTCCACAAAG AACTGGCGCT TCCTCTGTTA GCCTTCTTTC TGTCCTGCGA 
CAGCAAGAGA CTTCCTTTCA ATCACCTGTT TACACTTCTG CTAGTGATAG ATGGGGCAGC 
ACACCGGGAA CTAGTTCCAG CAGGCACCGT CCTCCAGAAG AAGAGGGACA GAGCAGCTCC 
TCTTCCATCC ACAGTGTCCT GCGTTGTAAC CTTTACCGCT ATTTTATGGA TTATGAGGGA 
ACGCAGGACA CGGTCCAGCC TCTTGATGGC AGCAGACAGG ACCAGCAGAC TCAGGAAATG 
CTCAACAACA ACATGGACCC AGAGCAACCA GGACCATCTC ATTACCAGTC ACCCTATAGC 
GGTGAAAACC CTCCCCACAG TCACATGAAC CGCTGTAGAG TATGCCACAA TCTTTTCACC 
TACAATCAGG GTAGCCGGCG CTGGGACAGA ACAGGTCAGC CTTCATCTAC TGAGAGAAAC 
ACACCATGGC AACCTTCTAG TTCTGCCTTC CACAGCGTAG CTCCAGTTTC ACAATCTAAT 
GAACACCTAC TGGAACACAG ACCCATAGAG TCCACACCAA ATACCCCAGA GCCTCATGTA 
CCATTTTCCC AGAGAACTGA CACTGGTCAG CATGAGGAGC AGGCCGTTGG GCTTGTCTTT 
AACCAGGAAA CTGGCCAACT GGAGCGTGTT TACAGACAGT CTGCCTCCTC TCGCTCTGCA 
AACATATCAC AAGGAGCCTT AAACCAGGAA ATGCCTGAAG ACACACCAGA TAACGATTAC 
TTGCGCAGGA GGCTGCTGGA GTCGTCCATG ATGTCTCTTT CGAGAAATGG CACCTCTGGG 
TCACGAGACC ACCCCATCTA TCCCGACCCA GCCAGGTTGT CCCCTGCAGC ATATTATGCA 
CAGCGTATGA TCCAGTATCT GTCTCGACGG GACAGCGTTC GGCAGCACTC CCACCGTCCC 
CCTAGCAGAC CTCGACCCCT GTCCTCTAAT CCCAGCAGTC TTTCCCCGAG CCCTGTCCCT 
AACGCAGAGA GCAGCGAAGT TGACTTTGAG GAGTTTGAGG AAAATGGAAG CAGATATCGG 
ACTCCTCGTA ATGCTCGCAT GTCTGCCCCC TCTCTTGGTC GTTTTGTTGG AACCAGGCGC 
TTTCTCTTGC CGGAGTTTTT GCCGTATGCT GGAATCTTCC ATGAGCGAGG GCAGCCAGGC 
CTGGCAACGC ACTCATCCGT CAATAGGGTC CTCGCAGGTG CTGTAATTGG AGACGGTCAA 
TCTGCAGTGG CCAGCAACAT CGCTAACACT ACCTATCGTC TGCAGTGGTG GGACTTCACC 
AAATTTGACT TGCCTGAAAT CAGTAATGCG TCGGTGAACG TTCTGGTGCC TAACTGTAAG 
ATCTACAACG ATGCGAGCTG TGATATCTCA GCGGATGGGC AGTTGCTGGC CGTATTCATT 
CCCAGCAGTC AGAGGGGTTT CCCTGATGAG GGCATTCTGG CTGTTTACTC TCTCGCTCCG 
CACAACCTGG GAGAGATGCT CTACTCCAAA AGATTCGGTC CAAATGCCAT CTCTGTCAGT 
TTGTCTCCGA TGGGTCGTTA CGTGATGGTG GGTCTGGCGT CTCGCCGCAT CCTGCTGCAT 
CAGATCAGCG ATCACATGGT GGCACAGGTC TTCAGACTGC AGCAGCCTCA CGCAGGAGAG 
ACGTCCATGA GGAGGGTGTT TGATGTGGTG TACCCAATGG CACCAGACCA GAGGAGACAC 
GTCAGTATAA ACTCTGCCCG CTGGCTGCCA GATCCGGGGC TTGGGCTCGC TTATGGGACC 
AACAAAGGAG ATCTTGTCAT CTGCAGACCT GTCGATGTGC ATTCTGATGG CAGCAGCACC 
TCTGAACACT CTGAGCGAAT GTTCACCATC AATAATGGTG GAGGGGTTGG ACCCAGTAGC 
AGCAGGAGTG GAGACAGGGC AGGGAGCAGC AGGACTGATC GACGGTCACG CAGAGACATA 
GGCCTGATGA ATGGAGTTGG TCTGCAGCCA CAACCTCCCG CCGCTAGTGT TACATCACAG 
GGGACGCAGA CACAGAACCA GCGACTACAG CATGCAGAGA CACAGACCGA CCGCGATCTT 
CCAGATGACC CCCAGCAGCC CAGCACTTCT CAGGGGTCAC AGGTCACTGA TGCCACAGAA 
TCTCTGGATT TTGAGACTCT GCCAGAGGAT TCTGGATCTG AGGTGGTACC CGAGACACCA 
CCCCACAGCC GGCCTCAGGA AGACGAAGGG TCAGATCCCA GCGAACCCAG CACAGACAGC 
ACAGGTCAGG CTGAATATGT TTCTCGTATC CGAAGGTTAA TGGCTGAGGG AGGCATGACT 
GCGGTTGTCC AGCGTGAGCA GAGCACCACA ATGGCGTCCA TGGGCAGCTT TGGCAACAAT 
ATCATCGTCA GCCATCGCAT CCACCGAGGC TCCCAGACAG GAGCGGATGC ACAAAATCGT 
ACTCGTTTAA GTCCAATACC TGGTCCTTCT TCTGGTGCTC CGGAGTCTTT AGCTGCAGCT 
TCGTATTCGC GCGTCCTCAC TAATACATTA GGCTTTCGTG GCGACACAGC TCAAGGCATA 
GATCTCACAG AACAGGAGAG ACTGCACACA TCCTTCTTTA CACCGGAGTT CTCTCCTCTT 
TTCTCATCTG CTGTCGATGC AACCGGTCCT TCCTCCTCAA TAGGAGCAGA CAGCGTCCTG 
GAGGGAGAAG ACTTTCACGA CTTTGCGTCT CTCCCTCCAT CTTTACTCTC ATCCTCGCCG 
TCTCTTTCAC CAGTGAATAA CAGTAACTAC AGCAACAGCG ATAGTAGTTA TCTTGGAGAT 
GAGTACGGGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of the Apelin/Elabela Receptors (APLNR) in Chickens, Turtles, and Zebrafish: Identification of a Novel Apelin-Specific Receptor in Teleosts.
Frontiers in endocrinology9756(2018)
Zhang J,Zhou Y,Wu C,Wan Y,Fang C,Li J,Fang W,Yi R,Zhu G,Li J,Wang Y

Effects of diisononyl phthalate on Danio rerio reproduction.
Environmental pollution (Barking, Essex : 1987)231(Pt 1)1051-1062(2017 Dec)
Santangeli S,Maradonna F,Zanardini M,Notarstefano V,Gioacchini G,Forner-Piquer I,Habibi H,Carnevali O

BPA-Induced Deregulation Of Epigenetic Patterns: Effects On Female Zebrafish Reproduction.
Scientific reports621982(2016 Feb)
Santangeli S,Maradonna F,Gioacchini G,Cobellis G,Piccinetti CC,Dalla Valle L,Carnevali O

Characterization of Ambra1 in asexual cycle of a non-vertebrate chordate, the colonial tunicate Botryllus schlosseri, and phylogenetic analysis of the protein group in Bilateria.
Molecular phylogenetics and evolution9546-57(2016 Feb)
Gasparini F,Skobo T,Benato F,Gioacchini G,Voskoboynik A,Carnevali O,Manni L,Dalla Valle L

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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ambra1a cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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