Dtx4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dtx4
Entrez Gene ID	207521
Full Name	deltex 4, E3 ubiquitin ligase
Synonyms	AI449438,BC044798,D930042H13,RNF155
General protein information	Preferred Names deltex 4, E3 ubiquitin ligase Names E3 ubiquitin-protein ligase DTX4 RING-type E3 ubiquitin transferase DTX4 deltex 4 homolog deltex4 protein deltex-4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
NM_172442.3	NP_766030.3	E3 ubiquitin-protein ligase DTX4
XM_006526816.2	XP_006526879.1	E3 ubiquitin-protein ligase DTX4 isoform X1
XM_006526816.3	XP_006526879.1	E3 ubiquitin-protein ligase DTX4 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DTX4	NP_055992.1
Rattus norvegicus (Norway rat)	Dtx4	NP_001041320.1
Bos taurus (cattle)	DTX4	XP_002693867.2
Pan troglodytes (chimpanzee)	DTX4	XP_508449.3
Macaca mulatta (Rhesus monkey)	DTX4	XP_001088765.1
Canis lupus familiaris (dog)	DTX4	XP_005631774.1
Mus musculus (house mouse)	Dtx4	NP_766030.3
Danio rerio (zebrafish)	dtx1	NP_001120780.1
Gallus gallus (chicken)	DTX4	XP_423367.4
Danio rerio (zebrafish)	zgc:163052	XP_002660570.1

Gene Ontology
Bibliography

Related articles in PubMed

E3 ubiquitin ligase DTX4 is required for adipogenic differentiation in 3T3-L1 preadipocytes cell line.
Wang Z, Dai Z, Pan Y, Wu S, Li Z, Zuo C
Biochemical and biophysical research communications492(3)419-424(2017 10)

Analogous mechanism regulating formation of neocortical basal radial glia and cerebellar Bergmann glia.
Heng X, Guo Q, Leung AW, Li JY
eLife6(2017 05)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Lumayag S, Haldin CE, Corbett NJ, Wahlin KJ, Cowan C, Turturro S, Larsen PE, Kovacs B, Witmer PD, Valle D, Zack DJ, Nicholson DA, Xu S
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)

Identification of molecules preferentially expressed beneath the marginal zone in the developing cerebral cortex.
Tachikawa K, Sasaki S, Maeda T, Nakajima K
Neuroscience research60(2)135-46(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dtx4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dtx4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03451	NM_172442.3 Latest version!	Mus musculus deltex 4, E3 ubiquitin ligase (Dtx4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu55346	XM_006526816.3 Latest version!	Mus musculus deltex 4, E3 ubiquitin ligase (Dtx4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu55346	XM_006526816.2	Mus musculus deltex 4, E3 ubiquitin ligase (Dtx4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dtx4 ( NM_172442.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766030.3 Mus musculus deltex 4, E3 ubiquitin ligase (Dtx4), mRNA.

Price & Availability↑

CloneID	OMu03451
Clone ID Related Accession (Same CDS sequence)	NM_172442.3
Accession Version	NM_172442.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1547913600000
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase DTX4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU705490.1, CF537541.1, BC058647.1, CA328050.1 and CN525673.1. On May 15, 2008 this sequence version replaced NM_172442.2. ##Evidence-Data-START## Transcript exon combination :: BC058647.1, SRR1660815.219521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTCCTGG CCTCGGCAGT GGTGGTGTGG GAATGGCTGA ACGAACACGG CCGCTGGCGT 
CCCTACAGCC CAGCCGTGAG TCACCACATC GAGGCGGTGG TGCGCGCTGG TCCCCGCGCC 
GGGGGCAGCG TGGTGTTGGG CCAGGTGGAC AGCCGGCTCG CGCCCTACAT CATCGACCTG 
CAGTCCATGA ACCAGTTCCG CCAAGACACG GGGACCCTCC GCCCAGTTCG ACGAAACTAC 
TATGACCCAT CCTCAGCCCC TGGTAAGGGA GTCGTGTGGG AATGGGAGAA TGACAATGGT 
TCCTGGACTC CCTATGACAT GGAAGTCGGC ATCACCATCC AGTATGCCTA TGAGAAGCAG 
CACCCATGGA TCGACCTCAC CTCCATTGGC TTCAGCTACA TAATAGACTT CAGCACCATG 
GGCCAGATCA ACAGGCAGAC CCAACGCCAG CGCCGTGTCA GACGACGACT AGACCTCATC 
TACCCCATGG TCACGGGCAC TATGCCAAAG ACTCAGTCCT GGCCAGTCAG TCCTGGACCG 
GCGACGTCCT CCCCTGCACC ACCTTGCTCC TGTCCACAGT GTGTTTTGGT GATGAGCGTC 
AAGGCTGCTG TGGTCCATGG GGGCACTGGG CCTCCAGCAG TACGAAAGAA CATGGCCCTC 
TCTGGAGTGG GCAAGCTGCC CCAGCCACCT GGCCCTGGAG CAAAGCCACT AGACACCACT 
GGCACCATTC GAGGCCCAGG GAAGACAGCC CCATCGCAGG TTATCAGAAG ACAAGTCTCT 
AATGCACCAG CTGGGGCGAC TGTAGGCTCC CCTGCCAGCC CACAAGGAAG CAACAGGAAG 
ACTGGAAGGG TGGCACTGGC CACTTTGAAT CGGTCCAATC TGCAGCGACT GGCCATTGCC 
CAGTCCCGGG TGCTGATTGC CTCCGGGGTT CCCACTGTGC CTGTGAAAAA CTTGAATGGG 
TCCAGCCCTG TTAATCCTGC CTTGGCAGGA ATCACCGGGA TACTCATGAG TGCAGCGGGA 
TTGCCTGTGT GCCTCACTCG CCCACCAAAG CTGGTCCTCC ACCCGCCCCC TGTCAGCAAG 
AGTGAGATAA AGTCCATCCC AGGGGTCTCG AACACGAGCC GCAAGACCAC CAAAAAGCAA 
GCCAAGAAAG GTAAAACCCC AGAGGAAGTG CTAAAGAAGT ACCTGCAGAA GGTCCGGCAC 
CCACCAGAAG AGGATTGTAC CATCTGTATG GAGCGTCTCA CAGCCCCCTC TGGCTACAAA 
GGCCCACAGC CTACAGTCAA GCCTGACCTG GTGGGAAAGC TGTCCAGGTG TGGCCACATC 
TACCACATCT ACTGCCTGGT GGCTATGTAC AACAATGGGA ACAAGGATGG GAGTTTACAG 
TGCCCAACAT GTAAGACCAT TTATGGCGTC AAGACAGGCA CCCAGCCTCC AGGGAAGATG 
GAATACCACC TCATCCCCCA CTCCTTGCCT GGCCATCCGG ACTGCAAGAC CATCCGGATC 
ATCTACAGTA TCCCCCCTGG CATTCAGGGA CCAGAGCATC CGAATCCTGG GAAGAGCTTC 
AGTGCCCGTG GCTTCCCACG ACATTGTTAC CTTCCAGACA GCGAGAAGGG GAGAAAAGTT 
CTGAAGCTGC TTCTAGTGGC CTGGGACCGC CGTCTCATCT TTGCCATTGG AACCTCCAGC 
ACCACGGGCG AGTCCGATAC TGTCATCTGG AATGAGGTTC ACCACAAAAC AGAATTTGGC 
TCTAATCTCA CTGGCCATGG CTACCCAGAT GCCAATTACC TGGATAACGT GCTGGCTGAA 
CTGGCTGCCC AGGGTATCTC TGAAGACAGC ACTTCCCATG AAAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766030.3
CDS	780..2630
Translation	MLLASAVVVWEWLNEHGRWRPYSPAVSHHIEAVVRAGPRAGGSVVLGQVDSRLAPYIIDLQSMNQFRQDTGTLRPVRRNYYDPSSAPGKGVVWEWENDNGSWTPYDMEVGITIQYAYEKQHPWIDLTSIGFSYIIDFSTMGQINRQTQRQRRVRRRLDLIYPMVTGTMPKTQSWPVSPGPATSSPAPPCSCPQCVLVMSVKAAVVHGGTGPPAVRKNMALSGVGKLPQPPGPGAKPLDTTGTIRGPGKTAPSQVIRRQVSNAPAGATVGSPASPQGSNRKTGRVALATLNRSNLQRLAIAQSRVLIASGVPTVPVKNLNGSSPVNPALAGITGILMSAAGLPVCLTRPPKLVLHPPPVSKSEIKSIPGVSNTSRKTTKKQAKKGKTPEEVLKKYLQKVRHPPEEDCTICMERLTAPSGYKGPQPTVKPDLVGKLSRCGHIYHIYCLVAMYNNGNKDGSLQCPTCKTIYGVKTGTQPPGKMEYHLIPHSLPGHPDCKTIRIIYSIPPGIQGPEHPNPGKSFSARGFPRHCYLPDSEKGRKVLKLLLVAWDRRLIFAIGTSSTTGESDTVIWNEVHHKTEFGSNLTGHGYPDANYLDNVLAELAAQGISEDSTSHEKD

Target ORF information:

RefSeq Version	NM_172442.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus deltex 4, E3 ubiquitin ligase (Dtx4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172442.3

ORF Insert Sequence:

ATGCTCCTGG CCTCGGCAGT GGTGGTGTGG GAATGGCTGA ACGAACACGG CCGCTGGCGT 
CCCTACAGCC CAGCCGTGAG TCACCACATC GAGGCGGTGG TGCGCGCTGG TCCCCGCGCC 
GGGGGCAGCG TGGTGTTGGG CCAGGTGGAC AGCCGGCTCG CGCCCTACAT CATCGACCTG 
CAGTCCATGA ACCAGTTCCG CCAAGACACG GGGACCCTCC GCCCAGTTCG ACGAAACTAC 
TATGACCCAT CCTCAGCCCC TGGTAAGGGA GTCGTGTGGG AATGGGAGAA TGACAATGGT 
TCCTGGACTC CCTATGACAT GGAAGTCGGC ATCACCATCC AGTATGCCTA TGAGAAGCAG 
CACCCATGGA TCGACCTCAC CTCCATTGGC TTCAGCTACA TAATAGACTT CAGCACCATG 
GGCCAGATCA ACAGGCAGAC CCAACGCCAG CGCCGTGTCA GACGACGACT AGACCTCATC 
TACCCCATGG TCACGGGCAC TATGCCAAAG ACTCAGTCCT GGCCAGTCAG TCCTGGACCG 
GCGACGTCCT CCCCTGCACC ACCTTGCTCC TGTCCACAGT GTGTTTTGGT GATGAGCGTC 
AAGGCTGCTG TGGTCCATGG GGGCACTGGG CCTCCAGCAG TACGAAAGAA CATGGCCCTC 
TCTGGAGTGG GCAAGCTGCC CCAGCCACCT GGCCCTGGAG CAAAGCCACT AGACACCACT 
GGCACCATTC GAGGCCCAGG GAAGACAGCC CCATCGCAGG TTATCAGAAG ACAAGTCTCT 
AATGCACCAG CTGGGGCGAC TGTAGGCTCC CCTGCCAGCC CACAAGGAAG CAACAGGAAG 
ACTGGAAGGG TGGCACTGGC CACTTTGAAT CGGTCCAATC TGCAGCGACT GGCCATTGCC 
CAGTCCCGGG TGCTGATTGC CTCCGGGGTT CCCACTGTGC CTGTGAAAAA CTTGAATGGG 
TCCAGCCCTG TTAATCCTGC CTTGGCAGGA ATCACCGGGA TACTCATGAG TGCAGCGGGA 
TTGCCTGTGT GCCTCACTCG CCCACCAAAG CTGGTCCTCC ACCCGCCCCC TGTCAGCAAG 
AGTGAGATAA AGTCCATCCC AGGGGTCTCG AACACGAGCC GCAAGACCAC CAAAAAGCAA 
GCCAAGAAAG GTAAAACCCC AGAGGAAGTG CTAAAGAAGT ACCTGCAGAA GGTCCGGCAC 
CCACCAGAAG AGGATTGTAC CATCTGTATG GAGCGTCTCA CAGCCCCCTC TGGCTACAAA 
GGCCCACAGC CTACAGTCAA GCCTGACCTG GTGGGAAAGC TGTCCAGGTG TGGCCACATC 
TACCACATCT ACTGCCTGGT GGCTATGTAC AACAATGGGA ACAAGGATGG GAGTTTACAG 
TGCCCAACAT GTAAGACCAT TTATGGCGTC AAGACAGGCA CCCAGCCTCC AGGGAAGATG 
GAATACCACC TCATCCCCCA CTCCTTGCCT GGCCATCCGG ACTGCAAGAC CATCCGGATC 
ATCTACAGTA TCCCCCCTGG CATTCAGGGA CCAGAGCATC CGAATCCTGG GAAGAGCTTC 
AGTGCCCGTG GCTTCCCACG ACATTGTTAC CTTCCAGACA GCGAGAAGGG GAGAAAAGTT 
CTGAAGCTGC TTCTAGTGGC CTGGGACCGC CGTCTCATCT TTGCCATTGG AACCTCCAGC 
ACCACGGGCG AGTCCGATAC TGTCATCTGG AATGAGGTTC ACCACAAAAC AGAATTTGGC 
TCTAATCTCA CTGGCCATGG CTACCCAGAT GCCAATTACC TGGATAACGT GCTGGCTGAA 
CTGGCTGCCC AGGGTATCTC TGAAGACAGC ACTTCCCATG AAAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dtx4 ( XM_006526816.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526879.1 Mus musculus deltex 4, E3 ubiquitin ligase (Dtx4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55346
Clone ID Related Accession (Same CDS sequence)	XM_006526816.2 , XM_006526816.3
Accession Version	XM_006526816.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase DTX4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006526816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTCG GCATCACCAT CCAGTATGCC TATGAGAAGC AGCACCCATG GATCGACCTC 
ACCTCCATTG GCTTCAGCTA CATAATAGAC TTCAGCACCA TGGGCCAGAT CAACAGGCAG 
ACCCAACGCC AGCGCCGTGT CAGACGACGA CTAGACCTCA TCTACCCCAT GGTCACGGGC 
ACTATGCCAA AGACTCAGTC CTGGCCAGTC AGTCCTGGAC CGGCGACGTC CTCCCCTGCA 
CCACCTTGCT CCTGTCCACA GTGTGTTTTG GTGATGAGCG TCAAGGCTGC TGTGGTCCAT 
GGGGGCACTG GGCCTCCAGC AGTACGAAAG AACATGGCCC TCTCTGGAGT GGGCAAGCTG 
CCCCAGCCAC CTGGCCCTGG AGCAAAGCCA CTAGACACCA CTGGCACCAT TCGAGGCCCA 
GGGAAGACAG CCCCATCGCA GGTTATCAGA AGACAAGTCT CTAATGCACC AGCTGGGGCG 
ACTGTAGGCT CCCCTGCCAG CCCACAAGGA AGCAACAGGA AGACTGGAAG GGTGGCACTG 
GCCACTTTGA ATCGGTCCAA TCTGCAGCGA CTGGCCATTG CCCAGTCCCG GGTGCTGATT 
GCCTCCGGGG TTCCCACTGT GCCTGTGAAA AACTTGAATG GGTCCAGCCC TGTTAATCCT 
GCCTTGGCAG GAATCACCGG GATACTCATG AGTGCAGCGG GATTGCCTGT GTGCCTCACT 
CGCCCACCAA AGCTGGTCCT CCACCCGCCC CCTGTCAGCA AGAGTGAGAT AAAGTCCATC 
CCAGGGGTCT CGAACACGAG CCGCAAGACC ACCAAAAAGC AAGCCAAGAA AGGTAAAACC 
CCAGAGGAAG TGCTAAAGAA GTACCTGCAG AAGGTCCGGC ACCCACCAGA AGAGGATTGT 
ACCATCTGTA TGGAGCGTCT CACAGCCCCC TCTGGCTACA AAGGCCCACA GCCTACAGTC 
AAGCCTGACC TGGTGGGAAA GCTGTCCAGG TGTGGCCACA TCTACCACAT CTACTGCCTG 
GTGGCTATGT ACAACAATGG GAACAAGGAT GGGAGTTTAC AGTGCCCAAC ATGTAAGACC 
ATTTATGGCG TCAAGACAGG CACCCAGCCT CCAGGGAAGA TGGAATACCA CCTCATCCCC 
CACTCCTTGC CTGGCCATCC GGACTGCAAG ACCATCCGGA TCATCTACAG TATCCCCCCT 
GGCATTCAGG GACCAGAGCA TCCGAATCCT GGGAAGAGCT TCAGTGCCCG TGGCTTCCCA 
CGACATTGTT ACCTTCCAGA CAGCGAGAAG GGGAGAAAAG TTCTGAAGCT GCTTCTAGTG 
GCCTGGGACC GCCGTCTCAT CTTTGCCATT GGAACCTCCA GCACCACGGG CGAGTCCGAT 
ACTGTCATCT GGAATGAGGT TCACCACAAA ACAGAATTTG GCTCTAATCT CACTGGCCAT 
GGCTACCCAG ATGCCAATTA CCTGGATAAC GTGCTGGCTG AACTGGCTGC CCAGGGTATC 
TCTGAAGACA GCACTTCCCA TGAAAAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526879.1
CDS	206..1738
Translation	MEVGITIQYAYEKQHPWIDLTSIGFSYIIDFSTMGQINRQTQRQRRVRRRLDLIYPMVTGTMPKTQSWPVSPGPATSSPAPPCSCPQCVLVMSVKAAVVHGGTGPPAVRKNMALSGVGKLPQPPGPGAKPLDTTGTIRGPGKTAPSQVIRRQVSNAPAGATVGSPASPQGSNRKTGRVALATLNRSNLQRLAIAQSRVLIASGVPTVPVKNLNGSSPVNPALAGITGILMSAAGLPVCLTRPPKLVLHPPPVSKSEIKSIPGVSNTSRKTTKKQAKKGKTPEEVLKKYLQKVRHPPEEDCTICMERLTAPSGYKGPQPTVKPDLVGKLSRCGHIYHIYCLVAMYNNGNKDGSLQCPTCKTIYGVKTGTQPPGKMEYHLIPHSLPGHPDCKTIRIIYSIPPGIQGPEHPNPGKSFSARGFPRHCYLPDSEKGRKVLKLLLVAWDRRLIFAIGTSSTTGESDTVIWNEVHHKTEFGSNLTGHGYPDANYLDNVLAELAAQGISEDSTSHEKD

Target ORF information:

RefSeq Version	XM_006526816.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus deltex 4, E3 ubiquitin ligase (Dtx4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526816.2

ORF Insert Sequence:

ATGGAAGTCG GCATCACCAT CCAGTATGCC TATGAGAAGC AGCACCCATG GATCGACCTC 
ACCTCCATTG GCTTCAGCTA CATAATAGAC TTCAGCACCA TGGGCCAGAT CAACAGGCAG 
ACCCAACGCC AGCGCCGTGT CAGACGACGA CTAGACCTCA TCTACCCCAT GGTCACGGGC 
ACTATGCCAA AGACTCAGTC CTGGCCAGTC AGTCCTGGAC CGGCGACGTC CTCCCCTGCA 
CCACCTTGCT CCTGTCCACA GTGTGTTTTG GTGATGAGCG TCAAGGCTGC TGTGGTCCAT 
GGGGGCACTG GGCCTCCAGC AGTACGAAAG AACATGGCCC TCTCTGGAGT GGGCAAGCTG 
CCCCAGCCAC CTGGCCCTGG AGCAAAGCCA CTAGACACCA CTGGCACCAT TCGAGGCCCA 
GGGAAGACAG CCCCATCGCA GGTTATCAGA AGACAAGTCT CTAATGCACC AGCTGGGGCG 
ACTGTAGGCT CCCCTGCCAG CCCACAAGGA AGCAACAGGA AGACTGGAAG GGTGGCACTG 
GCCACTTTGA ATCGGTCCAA TCTGCAGCGA CTGGCCATTG CCCAGTCCCG GGTGCTGATT 
GCCTCCGGGG TTCCCACTGT GCCTGTGAAA AACTTGAATG GGTCCAGCCC TGTTAATCCT 
GCCTTGGCAG GAATCACCGG GATACTCATG AGTGCAGCGG GATTGCCTGT GTGCCTCACT 
CGCCCACCAA AGCTGGTCCT CCACCCGCCC CCTGTCAGCA AGAGTGAGAT AAAGTCCATC 
CCAGGGGTCT CGAACACGAG CCGCAAGACC ACCAAAAAGC AAGCCAAGAA AGGTAAAACC 
CCAGAGGAAG TGCTAAAGAA GTACCTGCAG AAGGTCCGGC ACCCACCAGA AGAGGATTGT 
ACCATCTGTA TGGAGCGTCT CACAGCCCCC TCTGGCTACA AAGGCCCACA GCCTACAGTC 
AAGCCTGACC TGGTGGGAAA GCTGTCCAGG TGTGGCCACA TCTACCACAT CTACTGCCTG 
GTGGCTATGT ACAACAATGG GAACAAGGAT GGGAGTTTAC AGTGCCCAAC ATGTAAGACC 
ATTTATGGCG TCAAGACAGG CACCCAGCCT CCAGGGAAGA TGGAATACCA CCTCATCCCC 
CACTCCTTGC CTGGCCATCC GGACTGCAAG ACCATCCGGA TCATCTACAG TATCCCCCCT 
GGCATTCAGG GACCAGAGCA TCCGAATCCT GGGAAGAGCT TCAGTGCCCG TGGCTTCCCA 
CGACATTGTT ACCTTCCAGA CAGCGAGAAG GGGAGAAAAG TTCTGAAGCT GCTTCTAGTG 
GCCTGGGACC GCCGTCTCAT CTTTGCCATT GGAACCTCCA GCACCACGGG CGAGTCCGAT 
ACTGTCATCT GGAATGAGGT TCACCACAAA ACAGAATTTG GCTCTAATCT CACTGGCCAT 
GGCTACCCAG ATGCCAATTA CCTGGATAAC GTGCTGGCTG AACTGGCTGC CCAGGGTATC 
TCTGAAGACA GCACTTCCCA TGAAAAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dtx4 ( XM_006526816.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526879.1 Mus musculus deltex 4, E3 ubiquitin ligase (Dtx4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55346
Clone ID Related Accession (Same CDS sequence)	XM_006526816.2 , XM_006526816.3
Accession Version	XM_006526816.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase DTX4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526816.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTCG GCATCACCAT CCAGTATGCC TATGAGAAGC AGCACCCATG GATCGACCTC 
ACCTCCATTG GCTTCAGCTA CATAATAGAC TTCAGCACCA TGGGCCAGAT CAACAGGCAG 
ACCCAACGCC AGCGCCGTGT CAGACGACGA CTAGACCTCA TCTACCCCAT GGTCACGGGC 
ACTATGCCAA AGACTCAGTC CTGGCCAGTC AGTCCTGGAC CGGCGACGTC CTCCCCTGCA 
CCACCTTGCT CCTGTCCACA GTGTGTTTTG GTGATGAGCG TCAAGGCTGC TGTGGTCCAT 
GGGGGCACTG GGCCTCCAGC AGTACGAAAG AACATGGCCC TCTCTGGAGT GGGCAAGCTG 
CCCCAGCCAC CTGGCCCTGG AGCAAAGCCA CTAGACACCA CTGGCACCAT TCGAGGCCCA 
GGGAAGACAG CCCCATCGCA GGTTATCAGA AGACAAGTCT CTAATGCACC AGCTGGGGCG 
ACTGTAGGCT CCCCTGCCAG CCCACAAGGA AGCAACAGGA AGACTGGAAG GGTGGCACTG 
GCCACTTTGA ATCGGTCCAA TCTGCAGCGA CTGGCCATTG CCCAGTCCCG GGTGCTGATT 
GCCTCCGGGG TTCCCACTGT GCCTGTGAAA AACTTGAATG GGTCCAGCCC TGTTAATCCT 
GCCTTGGCAG GAATCACCGG GATACTCATG AGTGCAGCGG GATTGCCTGT GTGCCTCACT 
CGCCCACCAA AGCTGGTCCT CCACCCGCCC CCTGTCAGCA AGAGTGAGAT AAAGTCCATC 
CCAGGGGTCT CGAACACGAG CCGCAAGACC ACCAAAAAGC AAGCCAAGAA AGGTAAAACC 
CCAGAGGAAG TGCTAAAGAA GTACCTGCAG AAGGTCCGGC ACCCACCAGA AGAGGATTGT 
ACCATCTGTA TGGAGCGTCT CACAGCCCCC TCTGGCTACA AAGGCCCACA GCCTACAGTC 
AAGCCTGACC TGGTGGGAAA GCTGTCCAGG TGTGGCCACA TCTACCACAT CTACTGCCTG 
GTGGCTATGT ACAACAATGG GAACAAGGAT GGGAGTTTAC AGTGCCCAAC ATGTAAGACC 
ATTTATGGCG TCAAGACAGG CACCCAGCCT CCAGGGAAGA TGGAATACCA CCTCATCCCC 
CACTCCTTGC CTGGCCATCC GGACTGCAAG ACCATCCGGA TCATCTACAG TATCCCCCCT 
GGCATTCAGG GACCAGAGCA TCCGAATCCT GGGAAGAGCT TCAGTGCCCG TGGCTTCCCA 
CGACATTGTT ACCTTCCAGA CAGCGAGAAG GGGAGAAAAG TTCTGAAGCT GCTTCTAGTG 
GCCTGGGACC GCCGTCTCAT CTTTGCCATT GGAACCTCCA GCACCACGGG CGAGTCCGAT 
ACTGTCATCT GGAATGAGGT TCACCACAAA ACAGAATTTG GCTCTAATCT CACTGGCCAT 
GGCTACCCAG ATGCCAATTA CCTGGATAAC GTGCTGGCTG AACTGGCTGC CCAGGGTATC 
TCTGAAGACA GCACTTCCCA TGAAAAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526879.1
CDS	184..1716
Translation	MEVGITIQYAYEKQHPWIDLTSIGFSYIIDFSTMGQINRQTQRQRRVRRRLDLIYPMVTGTMPKTQSWPVSPGPATSSPAPPCSCPQCVLVMSVKAAVVHGGTGPPAVRKNMALSGVGKLPQPPGPGAKPLDTTGTIRGPGKTAPSQVIRRQVSNAPAGATVGSPASPQGSNRKTGRVALATLNRSNLQRLAIAQSRVLIASGVPTVPVKNLNGSSPVNPALAGITGILMSAAGLPVCLTRPPKLVLHPPPVSKSEIKSIPGVSNTSRKTTKKQAKKGKTPEEVLKKYLQKVRHPPEEDCTICMERLTAPSGYKGPQPTVKPDLVGKLSRCGHIYHIYCLVAMYNNGNKDGSLQCPTCKTIYGVKTGTQPPGKMEYHLIPHSLPGHPDCKTIRIIYSIPPGIQGPEHPNPGKSFSARGFPRHCYLPDSEKGRKVLKLLLVAWDRRLIFAIGTSSTTGESDTVIWNEVHHKTEFGSNLTGHGYPDANYLDNVLAELAAQGISEDSTSHEKD

Target ORF information:

RefSeq Version	XM_006526816.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus deltex 4, E3 ubiquitin ligase (Dtx4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526816.3

ORF Insert Sequence:

ATGGAAGTCG GCATCACCAT CCAGTATGCC TATGAGAAGC AGCACCCATG GATCGACCTC 
ACCTCCATTG GCTTCAGCTA CATAATAGAC TTCAGCACCA TGGGCCAGAT CAACAGGCAG 
ACCCAACGCC AGCGCCGTGT CAGACGACGA CTAGACCTCA TCTACCCCAT GGTCACGGGC 
ACTATGCCAA AGACTCAGTC CTGGCCAGTC AGTCCTGGAC CGGCGACGTC CTCCCCTGCA 
CCACCTTGCT CCTGTCCACA GTGTGTTTTG GTGATGAGCG TCAAGGCTGC TGTGGTCCAT 
GGGGGCACTG GGCCTCCAGC AGTACGAAAG AACATGGCCC TCTCTGGAGT GGGCAAGCTG 
CCCCAGCCAC CTGGCCCTGG AGCAAAGCCA CTAGACACCA CTGGCACCAT TCGAGGCCCA 
GGGAAGACAG CCCCATCGCA GGTTATCAGA AGACAAGTCT CTAATGCACC AGCTGGGGCG 
ACTGTAGGCT CCCCTGCCAG CCCACAAGGA AGCAACAGGA AGACTGGAAG GGTGGCACTG 
GCCACTTTGA ATCGGTCCAA TCTGCAGCGA CTGGCCATTG CCCAGTCCCG GGTGCTGATT 
GCCTCCGGGG TTCCCACTGT GCCTGTGAAA AACTTGAATG GGTCCAGCCC TGTTAATCCT 
GCCTTGGCAG GAATCACCGG GATACTCATG AGTGCAGCGG GATTGCCTGT GTGCCTCACT 
CGCCCACCAA AGCTGGTCCT CCACCCGCCC CCTGTCAGCA AGAGTGAGAT AAAGTCCATC 
CCAGGGGTCT CGAACACGAG CCGCAAGACC ACCAAAAAGC AAGCCAAGAA AGGTAAAACC 
CCAGAGGAAG TGCTAAAGAA GTACCTGCAG AAGGTCCGGC ACCCACCAGA AGAGGATTGT 
ACCATCTGTA TGGAGCGTCT CACAGCCCCC TCTGGCTACA AAGGCCCACA GCCTACAGTC 
AAGCCTGACC TGGTGGGAAA GCTGTCCAGG TGTGGCCACA TCTACCACAT CTACTGCCTG 
GTGGCTATGT ACAACAATGG GAACAAGGAT GGGAGTTTAC AGTGCCCAAC ATGTAAGACC 
ATTTATGGCG TCAAGACAGG CACCCAGCCT CCAGGGAAGA TGGAATACCA CCTCATCCCC 
CACTCCTTGC CTGGCCATCC GGACTGCAAG ACCATCCGGA TCATCTACAG TATCCCCCCT 
GGCATTCAGG GACCAGAGCA TCCGAATCCT GGGAAGAGCT TCAGTGCCCG TGGCTTCCCA 
CGACATTGTT ACCTTCCAGA CAGCGAGAAG GGGAGAAAAG TTCTGAAGCT GCTTCTAGTG 
GCCTGGGACC GCCGTCTCAT CTTTGCCATT GGAACCTCCA GCACCACGGG CGAGTCCGAT 
ACTGTCATCT GGAATGAGGT TCACCACAAA ACAGAATTTG GCTCTAATCT CACTGGCCAT 
GGCTACCCAG ATGCCAATTA CCTGGATAAC GTGCTGGCTG AACTGGCTGC CCAGGGTATC 
TCTGAAGACA GCACTTCCCA TGAAAAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

E3 ubiquitin ligase DTX4 is required for adipogenic differentiation in 3T3-L1 preadipocytes cell line.
Biochemical and biophysical research communications492(3)419-424(2017 10)
Wang Z,Dai Z,Pan Y,Wu S,Li Z,Zuo C

Analogous mechanism regulating formation of neocortical basal radial glia and cerebellar Bergmann glia.
eLife6(2017 05)
Heng X,Guo Q,Leung AW,Li JY

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)
Lumayag S,Haldin CE,Corbett NJ,Wahlin KJ,Cowan C,Turturro S,Larsen PE,Kovacs B,Witmer PD,Valle D,Zack DJ,Nicholson DA,Xu S

Identification of molecules preferentially expressed beneath the marginal zone in the developing cerebral cortex.
Neuroscience research60(2)135-46(2008 Feb)
Tachikawa K,Sasaki S,Maeda T,Nakajima K

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Dtx4 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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