Rasgrf2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rasgrf2
Entrez Gene ID	19418
Full Name	RAS protein-specific guanine nucleotide-releasing factor 2
Synonyms	6330417G04Rik,AW048350,Grf2,Ras-GRF2
General protein information	Preferred Names RAS protein-specific guanine nucleotide-releasing factor 2 Names ras-specific guanine nucleotide-releasing factor 2 ras guanine nucleotide exchange factor 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 C3\|13 47.43 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009027.3	NP_033053.2	ras-specific guanine nucleotide-releasing factor 2
NM_009027.4	NP_033053.2	ras-specific guanine nucleotide-releasing factor 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RASGRF2	XP_005618219.1
Danio rerio (zebrafish)	rasgrf2b	NP_001121705.1
Pan troglodytes (chimpanzee)	RASGRF2	XP_517672.2
Xenopus tropicalis (tropical clawed frog)	rasgrf2	XP_002942408.2
Homo sapiens (human)	RASGRF2	NP_008840.1
Mus musculus (house mouse)	Rasgrf2	NP_033053.2
Rattus norvegicus (Norway rat)	Rasgrf2	NP_446173.1
Gallus gallus (chicken)	RASGRF2	XP_004949517.1
Bos taurus (cattle)	RASGRF2	XP_002689419.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001238	XP_321917.5

Gene Ontology
Bibliography

Related articles in PubMed

The Inhibition of RasGRF2, But Not RasGRF1, Alters Cocaine Reward in Mice.
Bernardi RE, Olevska A, Morella I, Fasano S, Santos E, Brambilla R, Spanagel R
The Journal of neuroscience : the official journal of the Society for Neuroscience39(32)6325-6338(2019 Aug)

Vision and Locomotion Shape the Interactions between Neuron Types in Mouse Visual Cortex.
Dipoppa M, Ranson A, Krumin M, Pachitariu M, Carandini M, Harris KD
Neuron98(3)602-615.e8(2018 05)

Ras-GRF2 regulates nestin-positive stem cell density and onset of differentiation during adult neurogenesis in the mouse dentate gyrus.
Gómez C, Jimeno D, Fernández-Medarde A, García-Navas R, Calzada N, Santos E
Molecular and cellular neurosciences85127-147(2017 12)

An R-CaMP1.07 reporter mouse for cell-type-specific expression of a sensitive red fluorescent calcium indicator.
Bethge P, Carta S, Lorenzo DA, Egolf L, Goniotaki D, Madisen L, Voigt FF, Chen JL, Schneider B, Ohkura M, Nakai J, Zeng H, Aguzzi A, Helmchen F
PloS one12(6)e0179460(2017)

Long-Term Optical Access to an Estimated One Million Neurons in the Live Mouse Cortex.
Kim TH, Zhang Y, Lecoq J, Jung JC, Li J, Zeng H, Niell CM, Schnitzer MJ
Cell reports17(12)3385-3394(2016 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rasgrf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rasgrf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00010	NM_009027.4 Latest version!	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$699.30 $999.00
View Old Accession Versions >>
OMu00010	NM_009027.3	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$699.30 $999.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rasgrf2 ( NM_009027.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033053.2 Mus musculus RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2), mRNA.

Price & Availability↑

CloneID	OMu00010
Clone ID Related Accession (Same CDS sequence)	NM_009027.3 , NM_009027.4
Accession Version	NM_009027.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549555200000
Organism	Mus musculus(house mouse)
Product	ras-specific guanine nucleotide-releasing factor 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U67326.2 and AC167972.3. On Sep 17, 2009 this sequence version replaced NM_009027.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCAT GCTGGCGCGC 
AAGGAGGGCA CCAAACGCGG CTTCCTGAGC AAGAAGGCTG CAGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCTCTCTA CCAGAATGTG CTCTTTTACT TCGAAGGCGA GCAGAGCGGC 
CGGCCAGCGG GCATGTACCT TCTAGAGGGC TGCAGCTGTG AGCGGACGCC TGCACCACCC 
AGGACCAACG CAGGACCCGC AGGCGCCCGG GACGCTTTGG ACAAGCAGTA TTACTTCACT 
GTCCTTTTTG GGCACGATGG TCAAAAACCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGCC 
GGGAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTACG CGGACATTTT GATCGAGAGG 
GAAGTGCTAA TGCAGAAGTA CATTCATCTA GTTCAGATTG TGGAGACCGA GAAGATCGCC 
ACCAACCAAC TCCGACATCA ACTCGAAGAT CAAGATACCG AAATCGAAAG GCTTAAATCA 
GAGATTGTTG CTCTTAATAA GACCAAGGAA CGGATGCGTC CGTACCACGT CCATCAAGAG 
GAGGAGGATC CAGACATCAA GAAGATTAAG AAGGTTCAGA GCTTCATGAG AGGGTGGCTG 
TGTAGGAGGA AGTGGAAGAC CATCGTGCAG GACTACATTT GTTCTCCCCA TGCAGAGAGC 
ATGAGGAAGA GAAACCAGAT TGTGTTCACG ATGGTGGAGG CCGAGACGGA GTATGTACAC 
CAGCTTTACA TCCTGGTGAA CGGTTTCCTC AGGCCCCTGC GCATGGCAGC CAGCTCCAAG 
AAGCCCCCCA TTAACCACGA TGACGTCAGC AGCATTTTTC TCAACAGTGA AACAATCATG 
TTCCTTCATG AAATATTCCA TCAAGGACTA AAGGCAAGGC TGGCAAATTG GCCTACTTTG 
GTTTTAGCGG ATCTATTTGA TATTTTGCTG CCCATGCTGA ACATTTATCA AGAATTTGTA 
CGTAATCACC AGTACAGCCT CCAAGTACTT GCCAACTGTA AGCAAAACAG AGATTTTGAC 
AAGCTCTTAA AGCAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAGACGTTC 
TTGACCTACC CAATGTTTCA GATTCCCAGG TATATCATCA CGCTGCACGA ACTCCTGGCC 
CACACCCCCC ATGAACATGT GGAGAGGAAA AGTCTGGAGT TCGCTAAGTC CAAGCTGGAG 
GAACTGTCCA GAGTGATGCA TGATGAAGTC AGTGACACCG AGAACATACG GAAAAATCTC 
GCCATTGAGA GGATGATCGT AGAGGGCTGT GACATTTTGC TGGACACCAG CCAAACTTTT 
ATCCGACAAG GTTCCCTTAT TCAAGTGCCT TCTGTGGAGA GGGGGAAACT GAGTAAAGTC 
CGCCTGGGCT CGCTGTCCCT GAAAAAGGAA GGCGAGAGAC AGTGTTTCTT ATTCACGAAG 
CACTTTCTGA TTTGTACACG AAGTTCAGGA GGAAAGCTGC ATTTGCTCAA GACAGGTGGG 
GTTCTCTCCC TCATTCAGTG TACATTGATT GAGGAGCCCG ATGGAAGTGA CGATGACCCC 
AAAGGTTCTG GCCACATGTT TGGACATCTG GATTTTAAAA TCGTAGTGGA GCCTCCTGAC 
GCTGCCTCTT TCACTGTGGT CTTATTGGCA CCTTCACGCC AGGAGAAGGC TGCATGGATG 
AGCGACATCA GTCAGTGTGT GGACAATATA CGATGTAACG GATTGATGAC TATAGTCTTT 
GAAGAGAATT CCAAAGTTAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCACAAA 
GACGACACTG ACATCTGTTT CAGTAAAACA CTCAATTCCT GCAAAGTGCC CCAGATCCGC 
TACGCCAGCG TGGAGCGCCT CTTGGAGCGG TTGACGGACT TGCGGTTTCT GAGCATTGAT 
TTCCTCAACA CCTTCCTGCA CACCTACCGC ATTTTCACCA CGGCCACCGT AGTGCTGGCA 
AAGCTCTCAG ACATCTACAA GAGGCCTTTC ACCTCCATTC CTGTCAGGTC ATTAGAGTTA 
TTTTTTGCCA CAAGCCAGAA CAACAGAGAA CATTTGGTAG ATGGAAAATC CCCACGTCTC 
TGTCGCAAGT TCTCTTCCCC ACCTCCCCTG GCTGTGTCCA GAACATCCTC CCCAGTGAGG 
GCCAGAAAGC TGTCTTTGAC GTCTTCCTTG AACTCAAGGA TTGGAGCCTT GGACCTGACT 
AATTCCTCAT CTTCCAGTAG TCCCACCACC ACCACCCACA GCCCTGCTGC ATCCCCACCA 
CCGCACACTG CTGTCTTGGA GTCTGCACCA GCCGACAAAG CGGGAGATTC TGCAGACATG 
TCACCATGTA GATCCCCCAC AACTCCCCGG CACCTCCGCT ATCGGCAGCC TGGAGGACAG 
GTAGCCGACA GCGCCCACTG TTCTGTGTCA CCTGCTTCTG CTTTTGCAAT CGCCACAGCT 
GCAGCAGGAC ATGGGAGTCC ACCAGGATTT AACAACGAGA GAACATGTGA CAAGGAGTTC 
ATCATACGGA GAACAGCCAC CAATCGAGTC CTGAATGTCC TCCGCCACTG GGTCTCAAAG 
CATGCCCAGG ATTTTGAACT CAATAATGAA CTCAAAATGA ATGTTCTAAA CTTGCTAGAA 
GAAGTTCTAC GAGACCCGGA CCTTCTTCCT CAAGAAAGGA AAGCAACGGC GAACATCCTG 
AGGGCTCTTT CACAAGATGA TCAAGATGAT ATCCACCTAA AATTAGAGGA TATAATTCAA 
ATGACAGACT GTCCAAAGGC CGAGTGCTTT GAGACGTTGT CAGCCATGGA GCTGGCCGAG 
CAGATCACTC TCCTGGACCA CATTGTTTTC AGAAGCATCC CCTACGAAGA GTTTCTTGGG 
CAGGGCTGGA TGAAGCTTGA CAAGAACGAA AGGACGCCTT ACATCATGAA AACCAGCCAG 
CACTTCAATG AGATGAGTAA CCTGGTGGCC TCCCAGATAA TGAATTATGC CGACATCAGC 
TCCCGTGCCA ACGCCATTGA GAAGTGGGTG GCAGTGGCGG ACATTTGCCG ATGTCTCCAC 
AACTACAATG GTGTTCTGGA GATCACCTCA GCCTTAAACA GAAGTGCCAT CTACAGGCTG 
AAGAAAACCT GGGCCAAGGT GTCCAAGCAG ACAAAAGCTC TAATGGACAA ACTTCAGAAG 
ACTGTTTCAT CTGAAGGAAG ATTTAAAAAC CTCAGAGAGA CTCTCAAAAA CTGTAACCCC 
CCAGCTGTCC CTTACCTTGG GATGTACTTG ACAGATCTGG CATTCATTGA AGAAGGGACA 
CCGAACTTTA CCGAAGAGGG CCTTGTCAAT TTCTCCAAAA TGCGAATGAT ATCACACATT 
ATTCGAGAGA TACGCCAGTT CCAGCAGACC GCTTACCGCA TAGACCAGCA GCCAAAGGTC 
ATACAATACC TGCTTGACAA AGCCCTTGTC ATAGACGAAG ATTCACTCTA TGAGCTGTCA 
CTCAAAATTG AACCTCGACT CCCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033053.2
CDS	1..3570
Translation	MQKSVRYNEGHALYLAMLARKEGTKRGFLSKKAAEASRWHEKWFALYQNVLFYFEGEQSGRPAGMYLLEGCSCERTPAPPRTNAGPAGARDALDKQYYFTVLFGHDGQKPLELRCEEEQAGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIATNQLRHQLEDQDTEIERLKSEIVALNKTKERMRPYHVHQEEEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAETEYVHQLYILVNGFLRPLRMAASSKKPPINHDDVSSIFLNSETIMFLHEIFHQGLKARLANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIQCTLIEEPDGSDDDPKGSGHMFGHLDFKIVVEPPDAASFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTATVVLAKLSDIYKRPFTSIPVRSLELFFATSQNNREHLVDGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSSLNSRIGALDLTNSSSSSSPTTTTHSPAASPPPHTAVLESAPADKAGDSADMSPCRSPTTPRHLRYRQPGGQVADSAHCSVSPASAFAIATAAAGHGSPPGFNNERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDIHLKLEDIIQMTDCPKAECFETLSAMELAEQITLLDHIVFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNEMSNLVASQIMNYADISSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTAYRIDQQPKVIQYLLDKALVIDEDSLYELSLKIEPRLPA

Target ORF information:

RefSeq Version	NM_009027.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009027.3

ORF Insert Sequence:

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCAT GCTGGCGCGC 
AAGGAGGGCA CCAAACGCGG CTTCCTGAGC AAGAAGGCTG CAGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCTCTCTA CCAGAATGTG CTCTTTTACT TCGAAGGCGA GCAGAGCGGC 
CGGCCAGCGG GCATGTACCT TCTAGAGGGC TGCAGCTGTG AGCGGACGCC TGCACCACCC 
AGGACCAACG CAGGACCCGC AGGCGCCCGG GACGCTTTGG ACAAGCAGTA TTACTTCACT 
GTCCTTTTTG GGCACGATGG TCAAAAACCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGCC 
GGGAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTACG CGGACATTTT GATCGAGAGG 
GAAGTGCTAA TGCAGAAGTA CATTCATCTA GTTCAGATTG TGGAGACCGA GAAGATCGCC 
ACCAACCAAC TCCGACATCA ACTCGAAGAT CAAGATACCG AAATCGAAAG GCTTAAATCA 
GAGATTGTTG CTCTTAATAA GACCAAGGAA CGGATGCGTC CGTACCACGT CCATCAAGAG 
GAGGAGGATC CAGACATCAA GAAGATTAAG AAGGTTCAGA GCTTCATGAG AGGGTGGCTG 
TGTAGGAGGA AGTGGAAGAC CATCGTGCAG GACTACATTT GTTCTCCCCA TGCAGAGAGC 
ATGAGGAAGA GAAACCAGAT TGTGTTCACG ATGGTGGAGG CCGAGACGGA GTATGTACAC 
CAGCTTTACA TCCTGGTGAA CGGTTTCCTC AGGCCCCTGC GCATGGCAGC CAGCTCCAAG 
AAGCCCCCCA TTAACCACGA TGACGTCAGC AGCATTTTTC TCAACAGTGA AACAATCATG 
TTCCTTCATG AAATATTCCA TCAAGGACTA AAGGCAAGGC TGGCAAATTG GCCTACTTTG 
GTTTTAGCGG ATCTATTTGA TATTTTGCTG CCCATGCTGA ACATTTATCA AGAATTTGTA 
CGTAATCACC AGTACAGCCT CCAAGTACTT GCCAACTGTA AGCAAAACAG AGATTTTGAC 
AAGCTCTTAA AGCAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAGACGTTC 
TTGACCTACC CAATGTTTCA GATTCCCAGG TATATCATCA CGCTGCACGA ACTCCTGGCC 
CACACCCCCC ATGAACATGT GGAGAGGAAA AGTCTGGAGT TCGCTAAGTC CAAGCTGGAG 
GAACTGTCCA GAGTGATGCA TGATGAAGTC AGTGACACCG AGAACATACG GAAAAATCTC 
GCCATTGAGA GGATGATCGT AGAGGGCTGT GACATTTTGC TGGACACCAG CCAAACTTTT 
ATCCGACAAG GTTCCCTTAT TCAAGTGCCT TCTGTGGAGA GGGGGAAACT GAGTAAAGTC 
CGCCTGGGCT CGCTGTCCCT GAAAAAGGAA GGCGAGAGAC AGTGTTTCTT ATTCACGAAG 
CACTTTCTGA TTTGTACACG AAGTTCAGGA GGAAAGCTGC ATTTGCTCAA GACAGGTGGG 
GTTCTCTCCC TCATTCAGTG TACATTGATT GAGGAGCCCG ATGGAAGTGA CGATGACCCC 
AAAGGTTCTG GCCACATGTT TGGACATCTG GATTTTAAAA TCGTAGTGGA GCCTCCTGAC 
GCTGCCTCTT TCACTGTGGT CTTATTGGCA CCTTCACGCC AGGAGAAGGC TGCATGGATG 
AGCGACATCA GTCAGTGTGT GGACAATATA CGATGTAACG GATTGATGAC TATAGTCTTT 
GAAGAGAATT CCAAAGTTAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCACAAA 
GACGACACTG ACATCTGTTT CAGTAAAACA CTCAATTCCT GCAAAGTGCC CCAGATCCGC 
TACGCCAGCG TGGAGCGCCT CTTGGAGCGG TTGACGGACT TGCGGTTTCT GAGCATTGAT 
TTCCTCAACA CCTTCCTGCA CACCTACCGC ATTTTCACCA CGGCCACCGT AGTGCTGGCA 
AAGCTCTCAG ACATCTACAA GAGGCCTTTC ACCTCCATTC CTGTCAGGTC ATTAGAGTTA 
TTTTTTGCCA CAAGCCAGAA CAACAGAGAA CATTTGGTAG ATGGAAAATC CCCACGTCTC 
TGTCGCAAGT TCTCTTCCCC ACCTCCCCTG GCTGTGTCCA GAACATCCTC CCCAGTGAGG 
GCCAGAAAGC TGTCTTTGAC GTCTTCCTTG AACTCAAGGA TTGGAGCCTT GGACCTGACT 
AATTCCTCAT CTTCCAGTAG TCCCACCACC ACCACCCACA GCCCTGCTGC ATCCCCACCA 
CCGCACACTG CTGTCTTGGA GTCTGCACCA GCCGACAAAG CGGGAGATTC TGCAGACATG 
TCACCATGTA GATCCCCCAC AACTCCCCGG CACCTCCGCT ATCGGCAGCC TGGAGGACAG 
GTAGCCGACA GCGCCCACTG TTCTGTGTCA CCTGCTTCTG CTTTTGCAAT CGCCACAGCT 
GCAGCAGGAC ATGGGAGTCC ACCAGGATTT AACAACGAGA GAACATGTGA CAAGGAGTTC 
ATCATACGGA GAACAGCCAC CAATCGAGTC CTGAATGTCC TCCGCCACTG GGTCTCAAAG 
CATGCCCAGG ATTTTGAACT CAATAATGAA CTCAAAATGA ATGTTCTAAA CTTGCTAGAA 
GAAGTTCTAC GAGACCCGGA CCTTCTTCCT CAAGAAAGGA AAGCAACGGC GAACATCCTG 
AGGGCTCTTT CACAAGATGA TCAAGATGAT ATCCACCTAA AATTAGAGGA TATAATTCAA 
ATGACAGACT GTCCAAAGGC CGAGTGCTTT GAGACGTTGT CAGCCATGGA GCTGGCCGAG 
CAGATCACTC TCCTGGACCA CATTGTTTTC AGAAGCATCC CCTACGAAGA GTTTCTTGGG 
CAGGGCTGGA TGAAGCTTGA CAAGAACGAA AGGACGCCTT ACATCATGAA AACCAGCCAG 
CACTTCAATG AGATGAGTAA CCTGGTGGCC TCCCAGATAA TGAATTATGC CGACATCAGC 
TCCCGTGCCA ACGCCATTGA GAAGTGGGTG GCAGTGGCGG ACATTTGCCG ATGTCTCCAC 
AACTACAATG GTGTTCTGGA GATCACCTCA GCCTTAAACA GAAGTGCCAT CTACAGGCTG 
AAGAAAACCT GGGCCAAGGT GTCCAAGCAG ACAAAAGCTC TAATGGACAA ACTTCAGAAG 
ACTGTTTCAT CTGAAGGAAG ATTTAAAAAC CTCAGAGAGA CTCTCAAAAA CTGTAACCCC 
CCAGCTGTCC CTTACCTTGG GATGTACTTG ACAGATCTGG CATTCATTGA AGAAGGGACA 
CCGAACTTTA CCGAAGAGGG CCTTGTCAAT TTCTCCAAAA TGCGAATGAT ATCACACATT 
ATTCGAGAGA TACGCCAGTT CCAGCAGACC GCTTACCGCA TAGACCAGCA GCCAAAGGTC 
ATACAATACC TGCTTGACAA AGCCCTTGTC ATAGACGAAG ATTCACTCTA TGAGCTGTCA 
CTCAAAATTG AACCTCGACT CCCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrf2 ( NM_009027.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033053.2 Mus musculus RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2), mRNA.

Price & Availability↑

CloneID	OMu00010
Clone ID Related Accession (Same CDS sequence)	NM_009027.3 , NM_009027.4
Accession Version	NM_009027.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572796800000
Organism	Mus musculus(house mouse)
Product	ras-specific guanine nucleotide-releasing factor 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC146698.10, U67326.2 and AC167972.3. On Jun 19, 2019 this sequence version replaced NM_009027.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK158472.1, U67326.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01164131, SAMN01164139 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCAT GCTGGCGCGC 
AAGGAGGGCA CCAAACGCGG CTTCCTGAGC AAGAAGGCTG CAGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCTCTCTA CCAGAATGTG CTCTTTTACT TCGAAGGCGA GCAGAGCGGC 
CGGCCAGCGG GCATGTACCT TCTAGAGGGC TGCAGCTGTG AGCGGACGCC TGCACCACCC 
AGGACCAACG CAGGACCCGC AGGCGCCCGG GACGCTTTGG ACAAGCAGTA TTACTTCACT 
GTCCTTTTTG GGCACGATGG TCAAAAACCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGCC 
GGGAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTACG CGGACATTTT GATCGAGAGG 
GAAGTGCTAA TGCAGAAGTA CATTCATCTA GTTCAGATTG TGGAGACCGA GAAGATCGCC 
ACCAACCAAC TCCGACATCA ACTCGAAGAT CAAGATACCG AAATCGAAAG GCTTAAATCA 
GAGATTGTTG CTCTTAATAA GACCAAGGAA CGGATGCGTC CGTACCACGT CCATCAAGAG 
GAGGAGGATC CAGACATCAA GAAGATTAAG AAGGTTCAGA GCTTCATGAG AGGGTGGCTG 
TGTAGGAGGA AGTGGAAGAC CATCGTGCAG GACTACATTT GTTCTCCCCA TGCAGAGAGC 
ATGAGGAAGA GAAACCAGAT TGTGTTCACG ATGGTGGAGG CCGAGACGGA GTATGTACAC 
CAGCTTTACA TCCTGGTGAA CGGTTTCCTC AGGCCCCTGC GCATGGCAGC CAGCTCCAAG 
AAGCCCCCCA TTAACCACGA TGACGTCAGC AGCATTTTTC TCAACAGTGA AACAATCATG 
TTCCTTCATG AAATATTCCA TCAAGGACTA AAGGCAAGGC TGGCAAATTG GCCTACTTTG 
GTTTTAGCGG ATCTATTTGA TATTTTGCTG CCCATGCTGA ACATTTATCA AGAATTTGTA 
CGTAATCACC AGTACAGCCT CCAAGTACTT GCCAACTGTA AGCAAAACAG AGATTTTGAC 
AAGCTCTTAA AGCAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAGACGTTC 
TTGACCTACC CAATGTTTCA GATTCCCAGG TATATCATCA CGCTGCACGA ACTCCTGGCC 
CACACCCCCC ATGAACATGT GGAGAGGAAA AGTCTGGAGT TCGCTAAGTC CAAGCTGGAG 
GAACTGTCCA GAGTGATGCA TGATGAAGTC AGTGACACCG AGAACATACG GAAAAATCTC 
GCCATTGAGA GGATGATCGT AGAGGGCTGT GACATTTTGC TGGACACCAG CCAAACTTTT 
ATCCGACAAG GTTCCCTTAT TCAAGTGCCT TCTGTGGAGA GGGGGAAACT GAGTAAAGTC 
CGCCTGGGCT CGCTGTCCCT GAAAAAGGAA GGCGAGAGAC AGTGTTTCTT ATTCACGAAG 
CACTTTCTGA TTTGTACACG AAGTTCAGGA GGAAAGCTGC ATTTGCTCAA GACAGGTGGG 
GTTCTCTCCC TCATTCAGTG TACATTGATT GAGGAGCCCG ATGGAAGTGA CGATGACCCC 
AAAGGTTCTG GCCACATGTT TGGACATCTG GATTTTAAAA TCGTAGTGGA GCCTCCTGAC 
GCTGCCTCTT TCACTGTGGT CTTATTGGCA CCTTCACGCC AGGAGAAGGC TGCATGGATG 
AGCGACATCA GTCAGTGTGT GGACAATATA CGATGTAACG GATTGATGAC TATAGTCTTT 
GAAGAGAATT CCAAAGTTAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCACAAA 
GACGACACTG ACATCTGTTT CAGTAAAACA CTCAATTCCT GCAAAGTGCC CCAGATCCGC 
TACGCCAGCG TGGAGCGCCT CTTGGAGCGG TTGACGGACT TGCGGTTTCT GAGCATTGAT 
TTCCTCAACA CCTTCCTGCA CACCTACCGC ATTTTCACCA CGGCCACCGT AGTGCTGGCA 
AAGCTCTCAG ACATCTACAA GAGGCCTTTC ACCTCCATTC CTGTCAGGTC ATTAGAGTTA 
TTTTTTGCCA CAAGCCAGAA CAACAGAGAA CATTTGGTAG ATGGAAAATC CCCACGTCTC 
TGTCGCAAGT TCTCTTCCCC ACCTCCCCTG GCTGTGTCCA GAACATCCTC CCCAGTGAGG 
GCCAGAAAGC TGTCTTTGAC GTCTTCCTTG AACTCAAGGA TTGGAGCCTT GGACCTGACT 
AATTCCTCAT CTTCCAGTAG TCCCACCACC ACCACCCACA GCCCTGCTGC ATCCCCACCA 
CCGCACACTG CTGTCTTGGA GTCTGCACCA GCCGACAAAG CGGGAGATTC TGCAGACATG 
TCACCATGTA GATCCCCCAC AACTCCCCGG CACCTCCGCT ATCGGCAGCC TGGAGGACAG 
GTAGCCGACA GCGCCCACTG TTCTGTGTCA CCTGCTTCTG CTTTTGCAAT CGCCACAGCT 
GCAGCAGGAC ATGGGAGTCC ACCAGGATTT AACAACGAGA GAACATGTGA CAAGGAGTTC 
ATCATACGGA GAACAGCCAC CAATCGAGTC CTGAATGTCC TCCGCCACTG GGTCTCAAAG 
CATGCCCAGG ATTTTGAACT CAATAATGAA CTCAAAATGA ATGTTCTAAA CTTGCTAGAA 
GAAGTTCTAC GAGACCCGGA CCTTCTTCCT CAAGAAAGGA AAGCAACGGC GAACATCCTG 
AGGGCTCTTT CACAAGATGA TCAAGATGAT ATCCACCTAA AATTAGAGGA TATAATTCAA 
ATGACAGACT GTCCAAAGGC CGAGTGCTTT GAGACGTTGT CAGCCATGGA GCTGGCCGAG 
CAGATCACTC TCCTGGACCA CATTGTTTTC AGAAGCATCC CCTACGAAGA GTTTCTTGGG 
CAGGGCTGGA TGAAGCTTGA CAAGAACGAA AGGACGCCTT ACATCATGAA AACCAGCCAG 
CACTTCAATG AGATGAGTAA CCTGGTGGCC TCCCAGATAA TGAATTATGC CGACATCAGC 
TCCCGTGCCA ACGCCATTGA GAAGTGGGTG GCAGTGGCGG ACATTTGCCG ATGTCTCCAC 
AACTACAATG GTGTTCTGGA GATCACCTCA GCCTTAAACA GAAGTGCCAT CTACAGGCTG 
AAGAAAACCT GGGCCAAGGT GTCCAAGCAG ACAAAAGCTC TAATGGACAA ACTTCAGAAG 
ACTGTTTCAT CTGAAGGAAG ATTTAAAAAC CTCAGAGAGA CTCTCAAAAA CTGTAACCCC 
CCAGCTGTCC CTTACCTTGG GATGTACTTG ACAGATCTGG CATTCATTGA AGAAGGGACA 
CCGAACTTTA CCGAAGAGGG CCTTGTCAAT TTCTCCAAAA TGCGAATGAT ATCACACATT 
ATTCGAGAGA TACGCCAGTT CCAGCAGACC GCTTACCGCA TAGACCAGCA GCCAAAGGTC 
ATACAATACC TGCTTGACAA AGCCCTTGTC ATAGACGAAG ATTCACTCTA TGAGCTGTCA 
CTCAAAATTG AACCTCGACT CCCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033053.2
CDS	335..3904
Translation	MQKSVRYNEGHALYLAMLARKEGTKRGFLSKKAAEASRWHEKWFALYQNVLFYFEGEQSGRPAGMYLLEGCSCERTPAPPRTNAGPAGARDALDKQYYFTVLFGHDGQKPLELRCEEEQAGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIATNQLRHQLEDQDTEIERLKSEIVALNKTKERMRPYHVHQEEEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAETEYVHQLYILVNGFLRPLRMAASSKKPPINHDDVSSIFLNSETIMFLHEIFHQGLKARLANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIQCTLIEEPDGSDDDPKGSGHMFGHLDFKIVVEPPDAASFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTATVVLAKLSDIYKRPFTSIPVRSLELFFATSQNNREHLVDGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSSLNSRIGALDLTNSSSSSSPTTTTHSPAASPPPHTAVLESAPADKAGDSADMSPCRSPTTPRHLRYRQPGGQVADSAHCSVSPASAFAIATAAAGHGSPPGFNNERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDIHLKLEDIIQMTDCPKAECFETLSAMELAEQITLLDHIVFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNEMSNLVASQIMNYADISSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTAYRIDQQPKVIQYLLDKALVIDEDSLYELSLKIEPRLPA

Target ORF information:

RefSeq Version	NM_009027.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009027.4

ORF Insert Sequence:

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCAT GCTGGCGCGC 
AAGGAGGGCA CCAAACGCGG CTTCCTGAGC AAGAAGGCTG CAGAGGCGAG CCGCTGGCAC 
GAGAAGTGGT TCGCTCTCTA CCAGAATGTG CTCTTTTACT TCGAAGGCGA GCAGAGCGGC 
CGGCCAGCGG GCATGTACCT TCTAGAGGGC TGCAGCTGTG AGCGGACGCC TGCACCACCC 
AGGACCAACG CAGGACCCGC AGGCGCCCGG GACGCTTTGG ACAAGCAGTA TTACTTCACT 
GTCCTTTTTG GGCACGATGG TCAAAAACCA CTGGAGCTGC GCTGTGAGGA GGAGCAGGCC 
GGGAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTACG CGGACATTTT GATCGAGAGG 
GAAGTGCTAA TGCAGAAGTA CATTCATCTA GTTCAGATTG TGGAGACCGA GAAGATCGCC 
ACCAACCAAC TCCGACATCA ACTCGAAGAT CAAGATACCG AAATCGAAAG GCTTAAATCA 
GAGATTGTTG CTCTTAATAA GACCAAGGAA CGGATGCGTC CGTACCACGT CCATCAAGAG 
GAGGAGGATC CAGACATCAA GAAGATTAAG AAGGTTCAGA GCTTCATGAG AGGGTGGCTG 
TGTAGGAGGA AGTGGAAGAC CATCGTGCAG GACTACATTT GTTCTCCCCA TGCAGAGAGC 
ATGAGGAAGA GAAACCAGAT TGTGTTCACG ATGGTGGAGG CCGAGACGGA GTATGTACAC 
CAGCTTTACA TCCTGGTGAA CGGTTTCCTC AGGCCCCTGC GCATGGCAGC CAGCTCCAAG 
AAGCCCCCCA TTAACCACGA TGACGTCAGC AGCATTTTTC TCAACAGTGA AACAATCATG 
TTCCTTCATG AAATATTCCA TCAAGGACTA AAGGCAAGGC TGGCAAATTG GCCTACTTTG 
GTTTTAGCGG ATCTATTTGA TATTTTGCTG CCCATGCTGA ACATTTATCA AGAATTTGTA 
CGTAATCACC AGTACAGCCT CCAAGTACTT GCCAACTGTA AGCAAAACAG AGATTTTGAC 
AAGCTCTTAA AGCAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAGACGTTC 
TTGACCTACC CAATGTTTCA GATTCCCAGG TATATCATCA CGCTGCACGA ACTCCTGGCC 
CACACCCCCC ATGAACATGT GGAGAGGAAA AGTCTGGAGT TCGCTAAGTC CAAGCTGGAG 
GAACTGTCCA GAGTGATGCA TGATGAAGTC AGTGACACCG AGAACATACG GAAAAATCTC 
GCCATTGAGA GGATGATCGT AGAGGGCTGT GACATTTTGC TGGACACCAG CCAAACTTTT 
ATCCGACAAG GTTCCCTTAT TCAAGTGCCT TCTGTGGAGA GGGGGAAACT GAGTAAAGTC 
CGCCTGGGCT CGCTGTCCCT GAAAAAGGAA GGCGAGAGAC AGTGTTTCTT ATTCACGAAG 
CACTTTCTGA TTTGTACACG AAGTTCAGGA GGAAAGCTGC ATTTGCTCAA GACAGGTGGG 
GTTCTCTCCC TCATTCAGTG TACATTGATT GAGGAGCCCG ATGGAAGTGA CGATGACCCC 
AAAGGTTCTG GCCACATGTT TGGACATCTG GATTTTAAAA TCGTAGTGGA GCCTCCTGAC 
GCTGCCTCTT TCACTGTGGT CTTATTGGCA CCTTCACGCC AGGAGAAGGC TGCATGGATG 
AGCGACATCA GTCAGTGTGT GGACAATATA CGATGTAACG GATTGATGAC TATAGTCTTT 
GAAGAGAATT CCAAAGTTAC TGTGCCACAT ATGATTAAGT CTGATGCCCG TCTTCACAAA 
GACGACACTG ACATCTGTTT CAGTAAAACA CTCAATTCCT GCAAAGTGCC CCAGATCCGC 
TACGCCAGCG TGGAGCGCCT CTTGGAGCGG TTGACGGACT TGCGGTTTCT GAGCATTGAT 
TTCCTCAACA CCTTCCTGCA CACCTACCGC ATTTTCACCA CGGCCACCGT AGTGCTGGCA 
AAGCTCTCAG ACATCTACAA GAGGCCTTTC ACCTCCATTC CTGTCAGGTC ATTAGAGTTA 
TTTTTTGCCA CAAGCCAGAA CAACAGAGAA CATTTGGTAG ATGGAAAATC CCCACGTCTC 
TGTCGCAAGT TCTCTTCCCC ACCTCCCCTG GCTGTGTCCA GAACATCCTC CCCAGTGAGG 
GCCAGAAAGC TGTCTTTGAC GTCTTCCTTG AACTCAAGGA TTGGAGCCTT GGACCTGACT 
AATTCCTCAT CTTCCAGTAG TCCCACCACC ACCACCCACA GCCCTGCTGC ATCCCCACCA 
CCGCACACTG CTGTCTTGGA GTCTGCACCA GCCGACAAAG CGGGAGATTC TGCAGACATG 
TCACCATGTA GATCCCCCAC AACTCCCCGG CACCTCCGCT ATCGGCAGCC TGGAGGACAG 
GTAGCCGACA GCGCCCACTG TTCTGTGTCA CCTGCTTCTG CTTTTGCAAT CGCCACAGCT 
GCAGCAGGAC ATGGGAGTCC ACCAGGATTT AACAACGAGA GAACATGTGA CAAGGAGTTC 
ATCATACGGA GAACAGCCAC CAATCGAGTC CTGAATGTCC TCCGCCACTG GGTCTCAAAG 
CATGCCCAGG ATTTTGAACT CAATAATGAA CTCAAAATGA ATGTTCTAAA CTTGCTAGAA 
GAAGTTCTAC GAGACCCGGA CCTTCTTCCT CAAGAAAGGA AAGCAACGGC GAACATCCTG 
AGGGCTCTTT CACAAGATGA TCAAGATGAT ATCCACCTAA AATTAGAGGA TATAATTCAA 
ATGACAGACT GTCCAAAGGC CGAGTGCTTT GAGACGTTGT CAGCCATGGA GCTGGCCGAG 
CAGATCACTC TCCTGGACCA CATTGTTTTC AGAAGCATCC CCTACGAAGA GTTTCTTGGG 
CAGGGCTGGA TGAAGCTTGA CAAGAACGAA AGGACGCCTT ACATCATGAA AACCAGCCAG 
CACTTCAATG AGATGAGTAA CCTGGTGGCC TCCCAGATAA TGAATTATGC CGACATCAGC 
TCCCGTGCCA ACGCCATTGA GAAGTGGGTG GCAGTGGCGG ACATTTGCCG ATGTCTCCAC 
AACTACAATG GTGTTCTGGA GATCACCTCA GCCTTAAACA GAAGTGCCAT CTACAGGCTG 
AAGAAAACCT GGGCCAAGGT GTCCAAGCAG ACAAAAGCTC TAATGGACAA ACTTCAGAAG 
ACTGTTTCAT CTGAAGGAAG ATTTAAAAAC CTCAGAGAGA CTCTCAAAAA CTGTAACCCC 
CCAGCTGTCC CTTACCTTGG GATGTACTTG ACAGATCTGG CATTCATTGA AGAAGGGACA 
CCGAACTTTA CCGAAGAGGG CCTTGTCAAT TTCTCCAAAA TGCGAATGAT ATCACACATT 
ATTCGAGAGA TACGCCAGTT CCAGCAGACC GCTTACCGCA TAGACCAGCA GCCAAAGGTC 
ATACAATACC TGCTTGACAA AGCCCTTGTC ATAGACGAAG ATTCACTCTA TGAGCTGTCA 
CTCAAAATTG AACCTCGACT CCCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Inhibition of RasGRF2, But Not RasGRF1, Alters Cocaine Reward in Mice.
The Journal of neuroscience : the official journal of the Society for Neuroscience39(32)6325-6338(2019 Aug)
Bernardi RE,Olevska A,Morella I,Fasano S,Santos E,Brambilla R,Spanagel R

Vision and Locomotion Shape the Interactions between Neuron Types in Mouse Visual Cortex.
Neuron98(3)602-615.e8(2018 05)
Dipoppa M,Ranson A,Krumin M,Pachitariu M,Carandini M,Harris KD

Ras-GRF2 regulates nestin-positive stem cell density and onset of differentiation during adult neurogenesis in the mouse dentate gyrus.
Molecular and cellular neurosciences85127-147(2017 12)
Gómez C,Jimeno D,Fernández-Medarde A,García-Navas R,Calzada N,Santos E

An R-CaMP1.07 reporter mouse for cell-type-specific expression of a sensitive red fluorescent calcium indicator.
PloS one12(6)e0179460(2017)
Bethge P,Carta S,Lorenzo DA,Egolf L,Goniotaki D,Madisen L,Voigt FF,Chen JL,Schneider B,Ohkura M,Nakai J,Zeng H,Aguzzi A,Helmchen F

Long-Term Optical Access to an Estimated One Million Neurons in the Live Mouse Cortex.
Cell reports17(12)3385-3394(2016 12)
Kim TH,Zhang Y,Lecoq J,Jung JC,Li J,Zeng H,Niell CM,Schnitzer MJ

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Rasgrf2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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