RASGRF2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASGRF2
Entrez Gene ID	524957
Full Name	Ras protein specific guanine nucleotide releasing factor 2
General protein information	Preferred Names Ras protein specific guanine nucleotide releasing factor 2 Names ras-specific guanine nucleotide-releasing factor 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015464370.1	XP_015319856.1	ras-specific guanine nucleotide-releasing factor 2
XM_024995303.1	XP_024851071.1	ras-specific guanine nucleotide-releasing factor 2 isoform X3
XM_024995302.1	XP_024851070.1	ras-specific guanine nucleotide-releasing factor 2 isoform X2
XM_002689373.6	XP_002689419.4	ras-specific guanine nucleotide-releasing factor 2 isoform X1
XM_002689373.5	XP_002689419.3	ras-specific guanine nucleotide-releasing factor 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RASGRF2	XP_005618219.1
Danio rerio (zebrafish)	rasgrf2b	NP_001121705.1
Pan troglodytes (chimpanzee)	RASGRF2	XP_517672.2
Xenopus tropicalis (tropical clawed frog)	rasgrf2	XP_002942408.2
Homo sapiens (human)	RASGRF2	NP_008840.1
Mus musculus (house mouse)	Rasgrf2	NP_033053.2
Rattus norvegicus (Norway rat)	Rasgrf2	NP_446173.1
Gallus gallus (chicken)	RASGRF2	XP_004949517.1
Bos taurus (cattle)	RASGRF2	XP_002689419.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001238	XP_321917.5

Gene Ontology

The following RASGRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa236801	XM_002689373.6 Latest version!	Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa236802	XM_024995302.1 Latest version!	Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa236803	XM_024995303.1 Latest version!	Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa90367	XM_015464370.1 Latest version!	Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa90367	XM_002689373.5 Latest version!	Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGRF2 ( XM_002689373.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002689419.4 Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa236801
Clone ID Related Accession (Same CDS sequence)	XM_002689373.6
Accession Version	XM_002689373.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002689373.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT CCTAGCCCGC 
AAAGAGGGCA CCAAGCGCGG CTTCCTGAGC AAGAAGGCAG CCGAGGCGAG CCGCTGGCAC 
GAAAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TTGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGGGCACT CGCGCCGCCC 
AGGGCCGGGG CCGGGCCGGG CGGCGCCCGG GACACGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCACGAAGG TCAGAAGCCA CTAGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGGCATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATCG CTCTTAATAA AACAAAGGAA CGAATGAGAC CTTACCAAAG CAATCAAGAA 
GATGAGGATC CAGACATCAA GAAGATAAAA AAGGTTCAGA GCTTCATGCG AGGGTGGTTA 
TGTAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA CGCCGAAAGC 
ATGAGGAAGA GAAACCAGAT CGTGTTCACC ATGGTGGAGG CCGAGTCGGA GTACGTTCAC 
CAGCTCTACA TCCTCGTCAA TGGCTTCCTG AGGCCCCTGC GCATGGCGGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TCAACAGTGA AACAATCATG 
TTTCTTCACG AAATATTTCA TCAAGGACTG AAGGCAAGAA TAGCCAACTG GCCTACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT CCAAGTGCTC GCCAATTGTA AGCAAAACAG AGACTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAGACGTTC 
TTGACATATC CAATGTTTCA GATCCCCAGG TATATCATCA CGCTTCATGA ACTCCTGGCT 
CATACACCCC ATGAGCACGT GGAGAGAAAA AGTCTGGAGT TTGCGAAATC CAAACTAGAG 
GAACTGTCCA GGGTCATGCA TGATGAAGTG AGTGACACCG AAAACATAAG GAAGAACCTT 
GCCATAGAAA GAATGATCGT CGAGGGCTGT GATATTTTGC TGGACACCAG CCAGACTTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GAGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CACTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT GTTTACAAAA 
CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTGC ATCTGCTCAA GACAGGTGGA 
GTTCTGTCTC TAATAGAATG TACCTTGGTT GAGGAGCCCG ATGCGAGCGA TGATGACTCC 
AAAGGTTCTG GCCAAGTGTT TGGACACTTG GATTTCAAAA TAGTGGTGGA GCCTGCAGAT 
GCTGCCCCTT TCACTGTGGT CTTGTTAGCA CCTTCCCGCC AGGAGAAAGC TGCCTGGACA 
AGCGACATCA GTCAGTGTGT GGACAACATA CGGTGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAAAATT CCAAAGTTAC TGTGCCACAT ATGATTAAGT CTGACGCCCG GCTTCACAAG 
GACGACACTG ACATTTGCTT CAGTAAAACC CTCAACTCCT GCAAAGTGCC GCAGATCCGG 
TATGCCAGCG TGGAGCGCCT CTTGGAGAGA CTGACCGACT TGCGGTTTCT TAGCATCGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGG ATTTTCACGA CAGCAGCTGT GGTGCTGGGG 
AAGCTTTCTG ACATATATAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAACTC 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACACTTGG TGGAAGGCAA ATCCCCACGC 
CTGTGTCGCA AATTCTCTTC CCCACCTCCA CTGGCTGTGT CCAGGACGTC CTCCCCAGTG 
AGGGCCAGAA AGCTGTCCTT GACTTCTCCC TTGAATTCAA GGATAGGCGC ACTGGATCTG 
ACCACCTCCT CAGCATCCAG CAGCCCCACC ACCACCCACA GCCCAGCTGC ATCCCCACCG 
CCTCACACTG GTAAAGTGCC GTTGGATCTG AGCAGAGGCC TTTCTTCTCC AGAACAAAGC 
CCGGGATCTG TAGAGGAGTC TGTTGATAAC CCCCGTGTGG ATCTGTGTAA CAAGCTAAAA 
CGAAGTATTC AGAGAGCAGC AGTCCTCGAG TCTGCGCCGG TGGACCGAGC AGGTGTGGAC 
AGCTCACCCG TAGCCGATGC CGCAGAACTG TCACCTTGCA GGTCACCCTC AACTCCTCGG 
CACCTCCGCT ACCGCCAGCC TGGAGGACAG ACGGCTGACA ACTCCCACTG CTCTGTTTCA 
CCGGCCTCTG CTTTTGCAAT CGCTACAGCT GCAGCAGGAC ACGGGAGTCC ACCCGGATTT 
AACAACTCCG AGAGAACATG CGACAAAGAG TTTATTATAC GTAGAACAGC CACCAATCGA 
GTACTGAACG TTCTCCGTCA CTGGGTCTCA AAGCACGCAC AGGACTTTGA ACTCAACAAT 
GAACTAAAGA TGAACGTCTT AAATTTGCTA GAAGAAGTTT TACGAGACCC AGATCTTCTT 
CCCCAAGAAC GGAAAGCCAC AGCGAACATC CTAAGGGCCC TTTCACAAGA TGATCAAGAT 
GACATCCACC TAAAACTAGA GGATATAATT CAGCTGACGG ACTGTCCGAA GGCCGAGTGC 
TTCGAGACCT TGTCGGCCAT GGAGCTGGCT GAGCAGATAA CCCTCCTGGA CCACATCGTT 
TTCAGAAGCA TTCCCTACGA AGAATTTCTT GGGCAGGGGT GGATGAAGCT GGATAAAAAT 
GAAAGAACAC CATACATTAT GAAAACCAGC CAACACTTCA ATGATATGAG TAACCTGGTG 
GCCTCCCAGA TCATGAATTA CGCGGATGTC ACCTCCCGAG CCAACACCAT CGAGAAGTGG 
GTGGCGGTGG CGGACATCTG CCGGTGCCTG CACAATTACA ACGGCGTGCT GGAGATCACC 
TCAGCCTTAA ACAGAAGTGC CATCTACCGG CTGAAGAAGA CCTGGGCCAA GGTGTCCAAG 
CAGACAAAAG CTCTAATGGA CAAGCTTCAG AAGACTGTTT CGTCTGAAGG AAGATTTAAA 
AACCTCAGAG AAACCCTTAA AAATTGTAAC CCCCCGGCTG TTCCTTATCT GGGGATGTAC 
CTGACAGACC TGGCGTTCAT TGAAGAAGGA ACACCAAACT TTACCGAGGA AGGCCTCGTC 
AATTTCTCCA AGATGAGAAT GATATCACAC ATCATCAGAG AGATACGCCA GTTCCAGCAG 
ACTTCCTATC GAATCGATCA TCAGCCAAAG GTCACTCAGT ACTTGCTTGA CAAAGCCCTC 
ATCATAGATG AAGATACGCT GTATGAGCTG TCACTAAAAA TTGAACCTCG GCTCCCTGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002689419.4
CDS	485..4207
Translation	MQKSVRYNEGHALYLAFLARKEGTKRGFLSKKAAEASRWHEKWFALYQNVLFYFEGEQSCRPAGMYLLEGCSCERALAPPRAGAGPGGARDTLDKQYYFTVLFGHEGQKPLELRCEEEQDGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIECTLVEEPDASDDDSKGSGQVFGHLDFKIVVEPADAAPFTVVLLAPSRQEKAAWTSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLELFFATSQNNRGEHLVEGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSPLNSRIGALDLTTSSASSSPTTTHSPAASPPPHTGKVPLDLSRGLSSPEQSPGSVEESVDNPRVDLCNKLKRSIQRAAVLESAPVDRAGVDSSPVADAAELSPCRSPSTPRHLRYRQPGGQTADNSHCSVSPASAFAIATAAAGHGSPPGFNNSERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDIHLKLEDIIQLTDCPKAECFETLSAMELAEQITLLDHIVFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVTSRANTIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIDHQPKVTQYLLDKALIIDEDTLYELSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_002689373.6
Organism	Bos taurus(cattle)
Definition	Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002689373.6

ORF Insert Sequence:

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT CCTAGCCCGC 
AAAGAGGGCA CCAAGCGCGG CTTCCTGAGC AAGAAGGCAG CCGAGGCGAG CCGCTGGCAC 
GAAAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TTGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGGGCACT CGCGCCGCCC 
AGGGCCGGGG CCGGGCCGGG CGGCGCCCGG GACACGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCACGAAGG TCAGAAGCCA CTAGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGGCATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATCG CTCTTAATAA AACAAAGGAA CGAATGAGAC CTTACCAAAG CAATCAAGAA 
GATGAGGATC CAGACATCAA GAAGATAAAA AAGGTTCAGA GCTTCATGCG AGGGTGGTTA 
TGTAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA CGCCGAAAGC 
ATGAGGAAGA GAAACCAGAT CGTGTTCACC ATGGTGGAGG CCGAGTCGGA GTACGTTCAC 
CAGCTCTACA TCCTCGTCAA TGGCTTCCTG AGGCCCCTGC GCATGGCGGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TCAACAGTGA AACAATCATG 
TTTCTTCACG AAATATTTCA TCAAGGACTG AAGGCAAGAA TAGCCAACTG GCCTACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT CCAAGTGCTC GCCAATTGTA AGCAAAACAG AGACTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAGACGTTC 
TTGACATATC CAATGTTTCA GATCCCCAGG TATATCATCA CGCTTCATGA ACTCCTGGCT 
CATACACCCC ATGAGCACGT GGAGAGAAAA AGTCTGGAGT TTGCGAAATC CAAACTAGAG 
GAACTGTCCA GGGTCATGCA TGATGAAGTG AGTGACACCG AAAACATAAG GAAGAACCTT 
GCCATAGAAA GAATGATCGT CGAGGGCTGT GATATTTTGC TGGACACCAG CCAGACTTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GAGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CACTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT GTTTACAAAA 
CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTGC ATCTGCTCAA GACAGGTGGA 
GTTCTGTCTC TAATAGAATG TACCTTGGTT GAGGAGCCCG ATGCGAGCGA TGATGACTCC 
AAAGGTTCTG GCCAAGTGTT TGGACACTTG GATTTCAAAA TAGTGGTGGA GCCTGCAGAT 
GCTGCCCCTT TCACTGTGGT CTTGTTAGCA CCTTCCCGCC AGGAGAAAGC TGCCTGGACA 
AGCGACATCA GTCAGTGTGT GGACAACATA CGGTGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAAAATT CCAAAGTTAC TGTGCCACAT ATGATTAAGT CTGACGCCCG GCTTCACAAG 
GACGACACTG ACATTTGCTT CAGTAAAACC CTCAACTCCT GCAAAGTGCC GCAGATCCGG 
TATGCCAGCG TGGAGCGCCT CTTGGAGAGA CTGACCGACT TGCGGTTTCT TAGCATCGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGG ATTTTCACGA CAGCAGCTGT GGTGCTGGGG 
AAGCTTTCTG ACATATATAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAACTC 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACACTTGG TGGAAGGCAA ATCCCCACGC 
CTGTGTCGCA AATTCTCTTC CCCACCTCCA CTGGCTGTGT CCAGGACGTC CTCCCCAGTG 
AGGGCCAGAA AGCTGTCCTT GACTTCTCCC TTGAATTCAA GGATAGGCGC ACTGGATCTG 
ACCACCTCCT CAGCATCCAG CAGCCCCACC ACCACCCACA GCCCAGCTGC ATCCCCACCG 
CCTCACACTG GTAAAGTGCC GTTGGATCTG AGCAGAGGCC TTTCTTCTCC AGAACAAAGC 
CCGGGATCTG TAGAGGAGTC TGTTGATAAC CCCCGTGTGG ATCTGTGTAA CAAGCTAAAA 
CGAAGTATTC AGAGAGCAGC AGTCCTCGAG TCTGCGCCGG TGGACCGAGC AGGTGTGGAC 
AGCTCACCCG TAGCCGATGC CGCAGAACTG TCACCTTGCA GGTCACCCTC AACTCCTCGG 
CACCTCCGCT ACCGCCAGCC TGGAGGACAG ACGGCTGACA ACTCCCACTG CTCTGTTTCA 
CCGGCCTCTG CTTTTGCAAT CGCTACAGCT GCAGCAGGAC ACGGGAGTCC ACCCGGATTT 
AACAACTCCG AGAGAACATG CGACAAAGAG TTTATTATAC GTAGAACAGC CACCAATCGA 
GTACTGAACG TTCTCCGTCA CTGGGTCTCA AAGCACGCAC AGGACTTTGA ACTCAACAAT 
GAACTAAAGA TGAACGTCTT AAATTTGCTA GAAGAAGTTT TACGAGACCC AGATCTTCTT 
CCCCAAGAAC GGAAAGCCAC AGCGAACATC CTAAGGGCCC TTTCACAAGA TGATCAAGAT 
GACATCCACC TAAAACTAGA GGATATAATT CAGCTGACGG ACTGTCCGAA GGCCGAGTGC 
TTCGAGACCT TGTCGGCCAT GGAGCTGGCT GAGCAGATAA CCCTCCTGGA CCACATCGTT 
TTCAGAAGCA TTCCCTACGA AGAATTTCTT GGGCAGGGGT GGATGAAGCT GGATAAAAAT 
GAAAGAACAC CATACATTAT GAAAACCAGC CAACACTTCA ATGATATGAG TAACCTGGTG 
GCCTCCCAGA TCATGAATTA CGCGGATGTC ACCTCCCGAG CCAACACCAT CGAGAAGTGG 
GTGGCGGTGG CGGACATCTG CCGGTGCCTG CACAATTACA ACGGCGTGCT GGAGATCACC 
TCAGCCTTAA ACAGAAGTGC CATCTACCGG CTGAAGAAGA CCTGGGCCAA GGTGTCCAAG 
CAGACAAAAG CTCTAATGGA CAAGCTTCAG AAGACTGTTT CGTCTGAAGG AAGATTTAAA 
AACCTCAGAG AAACCCTTAA AAATTGTAAC CCCCCGGCTG TTCCTTATCT GGGGATGTAC 
CTGACAGACC TGGCGTTCAT TGAAGAAGGA ACACCAAACT TTACCGAGGA AGGCCTCGTC 
AATTTCTCCA AGATGAGAAT GATATCACAC ATCATCAGAG AGATACGCCA GTTCCAGCAG 
ACTTCCTATC GAATCGATCA TCAGCCAAAG GTCACTCAGT ACTTGCTTGA CAAAGCCCTC 
ATCATAGATG AAGATACGCT GTATGAGCTG TCACTAAAAA TTGAACCTCG GCTCCCTGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2 ( XM_024995302.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851070.1 Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa236802
Clone ID Related Accession (Same CDS sequence)	XM_024995302.1
Accession Version	XM_024995302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT CCTAGCCCGC 
AAAGAGGGCA CCAAGCGCGG CTTCCTGAGC AAGAAGGCAG CCGAGGCGAG CCGCTGGCAC 
GAAAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TTGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGGGCACT CGCGCCGCCC 
AGGGCCGGGG CCGGGCCGGG CGGCGCCCGG GACACGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCACGAAGG TCAGAAGCCA CTAGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGGCATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATCG CTCTTAATAA AACAAAGGAA CGAATGAGAC CTTACCAAAG CAATCAAGAA 
GATGAGGATC CAGACATCAA GAAGATAAAA AAGGTTCAGA GCTTCATGCG AGGGTGGTTA 
TGTAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA CGCCGAAAGC 
ATGAGGAAGA GAAACCAGAT CGTGTTCACC ATGGTGGAGG CCGAGTCGGA GTACGTTCAC 
CAGCTCTACA TCCTCGTCAA TGGCTTCCTG AGGCCCCTGC GCATGGCGGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TCAACAGTGA AACAATCATG 
TTTCTTCACG AAATATTTCA TCAAGGACTG AAGGCAAGAA TAGCCAACTG GCCTACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT CCAAGTGCTC GCCAATTGTA AGCAAAACAG AGACTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAGACGTTC 
TTGACATATC CAATGTTTCA GATCCCCAGG TATATCATCA CGCTTCATGA ACTCCTGGCT 
CATACACCCC ATGAGCACGT GGAGAGAAAA AGTCTGGAGT TTGCGAAATC CAAACTAGAG 
GAACTGTCCA GGGTCATGCA TGATGAAGTG AGTGACACCG AAAACATAAG GAAGAACCTT 
GCCATAGAAA GAATGATCGT CGAGGGCTGT GATATTTTGC TGGACACCAG CCAGACTTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GAGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CACTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT GTTTACAAAA 
CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTGC ATCTGCTCAA GACAGGTGGA 
GTTCTGTCTC TAATAGAATG TACCTTGGTT GAGGAGCCCG ATGCGAGCGA TGATGACTCC 
AAAGGTTCTG GCCAAGTGTT TGGACACTTG GATTTCAAAA TAGTGGTGGA GCCTGCAGAT 
GCTGCCCCTT TCACTGTGGT CTTGTTAGCA CCTTCCCGCC AGGAGAAAGC TGCCTGGACA 
AGCGACATCA GTCAGTGTGT GGACAACATA CGGTGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAAAATT CCAAAGTTAC TGTGCCACAT ATGATTAAGT CTGACGCCCG GCTTCACAAG 
GACGACACTG ACATTTGCTT CAGTAAAACC CTCAACTCCT GCAAAGTGCC GCAGATCCGG 
TATGCCAGCG TGGAGCGCCT CTTGGAGAGA CTGACCGACT TGCGGTTTCT TAGCATCGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGG ATTTTCACGA CAGCAGCTGT GGTGCTGGGG 
AAGCTTTCTG ACATATATAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAACTC 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACACTTGG TGGAAGGCAA ATCCCCACGC 
CTGTGTCGCA AATTCTCTTC CCCACCTCCA CTGGCTGTGT CCAGGACGTC CTCCCCAGTG 
AGGGCCAGAA AGCTGTCCTT GACTTCTCCC TTGAATTCAA GGATAGGCGC ACTGGATCTG 
ACCACCTCCT CAGCATCCAG CAGCCCCACC ACCACCCACA GCCCAGCTGC ATCCCCACCG 
CCTCACACTG GTAAAGTGCC GTTGGATCTG AGCAGAGGCC TTTCTTCTCC AGAACAAAGC 
CCGGGATCTG TAGAGGAGTC TGTTGATAAC CCCCGTGTGG ATCTGTGTAA CAAGCTAAAA 
CGAAGTATTC AGAGAGCAGT CCTCGAGTCT GCGCCGGTGG ACCGAGCAGG TGTGGACAGC 
TCACCCGTAG CCGATGCCGC AGAACTGTCA CCTTGCAGGT CACCCTCAAC TCCTCGGCAC 
CTCCGCTACC GCCAGCCTGG AGGACAGACG GCTGACAACT CCCACTGCTC TGTTTCACCG 
GCCTCTGCTT TTGCAATCGC TACAGCTGCA GCAGGACACG GGAGTCCACC CGGATTTAAC 
AACTCCGAGA GAACATGCGA CAAAGAGTTT ATTATACGTA GAACAGCCAC CAATCGAGTA 
CTGAACGTTC TCCGTCACTG GGTCTCAAAG CACGCACAGG ACTTTGAACT CAACAATGAA 
CTAAAGATGA ACGTCTTAAA TTTGCTAGAA GAAGTTTTAC GAGACCCAGA TCTTCTTCCC 
CAAGAACGGA AAGCCACAGC GAACATCCTA AGGGCCCTTT CACAAGATGA TCAAGATGAC 
ATCCACCTAA AACTAGAGGA TATAATTCAG CTGACGGACT GTCCGAAGGC CGAGTGCTTC 
GAGACCTTGT CGGCCATGGA GCTGGCTGAG CAGATAACCC TCCTGGACCA CATCGTTTTC 
AGAAGCATTC CCTACGAAGA ATTTCTTGGG CAGGGGTGGA TGAAGCTGGA TAAAAATGAA 
AGAACACCAT ACATTATGAA AACCAGCCAA CACTTCAATG ATATGAGTAA CCTGGTGGCC 
TCCCAGATCA TGAATTACGC GGATGTCACC TCCCGAGCCA ACACCATCGA GAAGTGGGTG 
GCGGTGGCGG ACATCTGCCG GTGCCTGCAC AATTACAACG GCGTGCTGGA GATCACCTCA 
GCCTTAAACA GAAGTGCCAT CTACCGGCTG AAGAAGACCT GGGCCAAGGT GTCCAAGCAG 
ACAAAAGCTC TAATGGACAA GCTTCAGAAG ACTGTTTCGT CTGAAGGAAG ATTTAAAAAC 
CTCAGAGAAA CCCTTAAAAA TTGTAACCCC CCGGCTGTTC CTTATCTGGG GATGTACCTG 
ACAGACCTGG CGTTCATTGA AGAAGGAACA CCAAACTTTA CCGAGGAAGG CCTCGTCAAT 
TTCTCCAAGA TGAGAATGAT ATCACACATC ATCAGAGAGA TACGCCAGTT CCAGCAGACT 
TCCTATCGAA TCGATCATCA GCCAAAGGTC ACTCAGTACT TGCTTGACAA AGCCCTCATC 
ATAGATGAAG ATACGCTGTA TGAGCTGTCA CTAAAAATTG AACCTCGGCT CCCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851070.1
CDS	485..4204
Translation	MQKSVRYNEGHALYLAFLARKEGTKRGFLSKKAAEASRWHEKWFALYQNVLFYFEGEQSCRPAGMYLLEGCSCERALAPPRAGAGPGGARDTLDKQYYFTVLFGHEGQKPLELRCEEEQDGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIECTLVEEPDASDDDSKGSGQVFGHLDFKIVVEPADAAPFTVVLLAPSRQEKAAWTSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLELFFATSQNNRGEHLVEGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSPLNSRIGALDLTTSSASSSPTTTHSPAASPPPHTGKVPLDLSRGLSSPEQSPGSVEESVDNPRVDLCNKLKRSIQRAVLESAPVDRAGVDSSPVADAAELSPCRSPSTPRHLRYRQPGGQTADNSHCSVSPASAFAIATAAAGHGSPPGFNNSERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDIHLKLEDIIQLTDCPKAECFETLSAMELAEQITLLDHIVFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVTSRANTIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIDHQPKVTQYLLDKALIIDEDTLYELSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_024995302.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995302.1

ORF Insert Sequence:

ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGT ACCTGGCCTT CCTAGCCCGC 
AAAGAGGGCA CCAAGCGCGG CTTCCTGAGC AAGAAGGCAG CCGAGGCGAG CCGCTGGCAC 
GAAAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TTGAGGGCGA GCAGAGCTGC 
CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGGGCACT CGCGCCGCCC 
AGGGCCGGGG CCGGGCCGGG CGGCGCCCGG GACACGCTGG ACAAGCAGTA TTACTTTACT 
GTTCTTTTTG GCCACGAAGG TCAGAAGCCA CTAGAGCTGC GCTGTGAGGA GGAGCAGGAT 
GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG 
GAAGTATTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA 
GCTAACCAAC TCCGGCATCA ACTTGAAGAT CAAGACACAG AAATCGAAAG GCTTAAATCA 
GAGATTATCG CTCTTAATAA AACAAAGGAA CGAATGAGAC CTTACCAAAG CAATCAAGAA 
GATGAGGATC CAGACATCAA GAAGATAAAA AAGGTTCAGA GCTTCATGCG AGGGTGGTTA 
TGTAGAAGGA AATGGAAGAC CATCGTGCAG GATTACATTT GTTCTCCTCA CGCCGAAAGC 
ATGAGGAAGA GAAACCAGAT CGTGTTCACC ATGGTGGAGG CCGAGTCGGA GTACGTTCAC 
CAGCTCTACA TCCTCGTCAA TGGCTTCCTG AGGCCCCTGC GCATGGCGGC CAGCTCCAAG 
AAGCCCCCCA TCAGCCACGA CGACGTCAGC AGTATTTTTC TCAACAGTGA AACAATCATG 
TTTCTTCACG AAATATTTCA TCAAGGACTG AAGGCAAGAA TAGCCAACTG GCCTACTTTA 
ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGCTGA ACATTTATCA AGAATTTGTG 
CGTAATCACC AGTACAGCCT CCAAGTGCTC GCCAATTGTA AGCAAAACAG AGACTTTGAC 
AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAGACGTTC 
TTGACATATC CAATGTTTCA GATCCCCAGG TATATCATCA CGCTTCATGA ACTCCTGGCT 
CATACACCCC ATGAGCACGT GGAGAGAAAA AGTCTGGAGT TTGCGAAATC CAAACTAGAG 
GAACTGTCCA GGGTCATGCA TGATGAAGTG AGTGACACCG AAAACATAAG GAAGAACCTT 
GCCATAGAAA GAATGATCGT CGAGGGCTGT GATATTTTGC TGGACACCAG CCAGACTTTC 
ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCCGTTGAGA GAGGGAAACT TAGTAAAGTT 
CGCCTGGGTT CACTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT GTTTACAAAA 
CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTGC ATCTGCTCAA GACAGGTGGA 
GTTCTGTCTC TAATAGAATG TACCTTGGTT GAGGAGCCCG ATGCGAGCGA TGATGACTCC 
AAAGGTTCTG GCCAAGTGTT TGGACACTTG GATTTCAAAA TAGTGGTGGA GCCTGCAGAT 
GCTGCCCCTT TCACTGTGGT CTTGTTAGCA CCTTCCCGCC AGGAGAAAGC TGCCTGGACA 
AGCGACATCA GTCAGTGTGT GGACAACATA CGGTGTAATG GTTTAATGAC TATAGTGTTT 
GAAGAAAATT CCAAAGTTAC TGTGCCACAT ATGATTAAGT CTGACGCCCG GCTTCACAAG 
GACGACACTG ACATTTGCTT CAGTAAAACC CTCAACTCCT GCAAAGTGCC GCAGATCCGG 
TATGCCAGCG TGGAGCGCCT CTTGGAGAGA CTGACCGACT TGCGGTTTCT TAGCATCGAT 
TTCCTCAACA CCTTTCTGCA CACCTATCGG ATTTTCACGA CAGCAGCTGT GGTGCTGGGG 
AAGCTTTCTG ACATATATAA GAGGCCTTTC ACCTCCATCC CTGTCAGGTC ATTGGAACTC 
TTTTTTGCTA CCAGCCAGAA CAACAGAGGT GAACACTTGG TGGAAGGCAA ATCCCCACGC 
CTGTGTCGCA AATTCTCTTC CCCACCTCCA CTGGCTGTGT CCAGGACGTC CTCCCCAGTG 
AGGGCCAGAA AGCTGTCCTT GACTTCTCCC TTGAATTCAA GGATAGGCGC ACTGGATCTG 
ACCACCTCCT CAGCATCCAG CAGCCCCACC ACCACCCACA GCCCAGCTGC ATCCCCACCG 
CCTCACACTG GTAAAGTGCC GTTGGATCTG AGCAGAGGCC TTTCTTCTCC AGAACAAAGC 
CCGGGATCTG TAGAGGAGTC TGTTGATAAC CCCCGTGTGG ATCTGTGTAA CAAGCTAAAA 
CGAAGTATTC AGAGAGCAGT CCTCGAGTCT GCGCCGGTGG ACCGAGCAGG TGTGGACAGC 
TCACCCGTAG CCGATGCCGC AGAACTGTCA CCTTGCAGGT CACCCTCAAC TCCTCGGCAC 
CTCCGCTACC GCCAGCCTGG AGGACAGACG GCTGACAACT CCCACTGCTC TGTTTCACCG 
GCCTCTGCTT TTGCAATCGC TACAGCTGCA GCAGGACACG GGAGTCCACC CGGATTTAAC 
AACTCCGAGA GAACATGCGA CAAAGAGTTT ATTATACGTA GAACAGCCAC CAATCGAGTA 
CTGAACGTTC TCCGTCACTG GGTCTCAAAG CACGCACAGG ACTTTGAACT CAACAATGAA 
CTAAAGATGA ACGTCTTAAA TTTGCTAGAA GAAGTTTTAC GAGACCCAGA TCTTCTTCCC 
CAAGAACGGA AAGCCACAGC GAACATCCTA AGGGCCCTTT CACAAGATGA TCAAGATGAC 
ATCCACCTAA AACTAGAGGA TATAATTCAG CTGACGGACT GTCCGAAGGC CGAGTGCTTC 
GAGACCTTGT CGGCCATGGA GCTGGCTGAG CAGATAACCC TCCTGGACCA CATCGTTTTC 
AGAAGCATTC CCTACGAAGA ATTTCTTGGG CAGGGGTGGA TGAAGCTGGA TAAAAATGAA 
AGAACACCAT ACATTATGAA AACCAGCCAA CACTTCAATG ATATGAGTAA CCTGGTGGCC 
TCCCAGATCA TGAATTACGC GGATGTCACC TCCCGAGCCA ACACCATCGA GAAGTGGGTG 
GCGGTGGCGG ACATCTGCCG GTGCCTGCAC AATTACAACG GCGTGCTGGA GATCACCTCA 
GCCTTAAACA GAAGTGCCAT CTACCGGCTG AAGAAGACCT GGGCCAAGGT GTCCAAGCAG 
ACAAAAGCTC TAATGGACAA GCTTCAGAAG ACTGTTTCGT CTGAAGGAAG ATTTAAAAAC 
CTCAGAGAAA CCCTTAAAAA TTGTAACCCC CCGGCTGTTC CTTATCTGGG GATGTACCTG 
ACAGACCTGG CGTTCATTGA AGAAGGAACA CCAAACTTTA CCGAGGAAGG CCTCGTCAAT 
TTCTCCAAGA TGAGAATGAT ATCACACATC ATCAGAGAGA TACGCCAGTT CCAGCAGACT 
TCCTATCGAA TCGATCATCA GCCAAAGGTC ACTCAGTACT TGCTTGACAA AGCCCTCATC 
ATAGATGAAG ATACGCTGTA TGAGCTGTCA CTAAAAATTG AACCTCGGCT CCCTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2 ( XM_024995303.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851071.1 Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X4, mRNA.

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CloneID	OBa236803
Clone ID Related Accession (Same CDS sequence)	XM_024995303.1
Accession Version	XM_024995303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGT ATTACTTTAC TGTTCTTTTT GGCCACGAAG GTCAGAAGCC ACTAGAGCTG 
CGCTGTGAGG AGGAGCAGGA TGGTAAAGAG TGGATGGAGG CCATTCACCA AGCCAGTTAT 
GCAGACATTT TGATTGAGAG GGAAGTATTA ATGCAGAAGT ACATTCATCT AGTTCAGATC 
GTAGAGACAG AAAAAATTGC AGCTAACCAA CTCCGGCATC AACTTGAAGA TCAAGACACA 
GAAATCGAAA GGCTTAAATC AGAGATTATC GCTCTTAATA AAACAAAGGA ACGAATGAGA 
CCTTACCAAA GCAATCAAGA AGATGAGGAT CCAGACATCA AGAAGATAAA AAAGGTTCAG 
AGCTTCATGC GAGGGTGGTT ATGTAGAAGG AAATGGAAGA CCATCGTGCA GGATTACATT 
TGTTCTCCTC ACGCCGAAAG CATGAGGAAG AGAAACCAGA TCGTGTTCAC CATGGTGGAG 
GCCGAGTCGG AGTACGTTCA CCAGCTCTAC ATCCTCGTCA ATGGCTTCCT GAGGCCCCTG 
CGCATGGCGG CCAGCTCCAA GAAGCCCCCC ATCAGCCACG ACGACGTCAG CAGTATTTTT 
CTCAACAGTG AAACAATCAT GTTTCTTCAC GAAATATTTC ATCAAGGACT GAAGGCAAGA 
ATAGCCAACT GGCCTACTTT AATTTTAGCT GATCTGTTTG ATATTTTGCT CCCCATGCTG 
AACATTTATC AAGAATTTGT GCGTAATCAC CAGTACAGCC TCCAAGTGCT CGCCAATTGT 
AAGCAAAACA GAGACTTTGA CAAACTCTTA AAACAGTATG AAGCCAACCC TGCCTGTGAG 
GGGAGGATGC TGGAGACGTT CTTGACATAT CCAATGTTTC AGATCCCCAG GTATATCATC 
ACGCTTCATG AACTCCTGGC TCATACACCC CATGAGCACG TGGAGAGAAA AAGTCTGGAG 
TTTGCGAAAT CCAAACTAGA GGAACTGTCC AGGGTCATGC ATGATGAAGT GAGTGACACC 
GAAAACATAA GGAAGAACCT TGCCATAGAA AGAATGATCG TCGAGGGCTG TGATATTTTG 
CTGGACACCA GCCAGACTTT CATCCGCCAA GGTTCTCTTA TTCAAGTACC TTCCGTTGAG 
AGAGGGAAAC TTAGTAAAGT TCGCCTGGGT TCACTGTCTT TGAAAAAGGA AGGAGAAAGA 
CAGTGCTTCT TGTTTACAAA ACACTTTTTA ATTTGTACAA GAAGTTCAGG AGGAAAGCTG 
CATCTGCTCA AGACAGGTGG AGTTCTGTCT CTAATAGAAT GTACCTTGGT TGAGGAGCCC 
GATGCGAGCG ATGATGACTC CAAAGGTTCT GGCCAAGTGT TTGGACACTT GGATTTCAAA 
ATAGTGGTGG AGCCTGCAGA TGCTGCCCCT TTCACTGTGG TCTTGTTAGC ACCTTCCCGC 
CAGGAGAAAG CTGCCTGGAC AAGCGACATC AGTCAGTGTG TGGACAACAT ACGGTGTAAT 
GGTTTAATGA CTATAGTGTT TGAAGAAAAT TCCAAAGTTA CTGTGCCACA TATGATTAAG 
TCTGACGCCC GGCTTCACAA GGACGACACT GACATTTGCT TCAGTAAAAC CCTCAACTCC 
TGCAAAGTGC CGCAGATCCG GTATGCCAGC GTGGAGCGCC TCTTGGAGAG ACTGACCGAC 
TTGCGGTTTC TTAGCATCGA TTTCCTCAAC ACCTTTCTGC ACACCTATCG GATTTTCACG 
ACAGCAGCTG TGGTGCTGGG GAAGCTTTCT GACATATATA AGAGGCCTTT CACCTCCATC 
CCTGTCAGGT CATTGGAACT CTTTTTTGCT ACCAGCCAGA ACAACAGAGG TGAACACTTG 
GTGGAAGGCA AATCCCCACG CCTGTGTCGC AAATTCTCTT CCCCACCTCC ACTGGCTGTG 
TCCAGGACGT CCTCCCCAGT GAGGGCCAGA AAGCTGTCCT TGACTTCTCC CTTGAATTCA 
AGGATAGGCG CACTGGATCT GACCACCTCC TCAGCATCCA GCAGCCCCAC CACCACCCAC 
AGCCCAGCTG CATCCCCACC GCCTCACACT GGTAAAGTGC CGTTGGATCT GAGCAGAGGC 
CTTTCTTCTC CAGAACAAAG CCCGGGATCT GTAGAGGAGT CTGTTGATAA CCCCCGTGTG 
GATCTGTGTA ACAAGCTAAA ACGAAGTATT CAGAGAGCAG CAGTCCTCGA GTCTGCGCCG 
GTGGACCGAG CAGGTGTGGA CAGCTCACCC GTAGCCGATG CCGCAGAACT GTCACCTTGC 
AGGTCACCCT CAACTCCTCG GCACCTCCGC TACCGCCAGC CTGGAGGACA GACGGCTGAC 
AACTCCCACT GCTCTGTTTC ACCGGCCTCT GCTTTTGCAA TCGCTACAGC TGCAGCAGGA 
CACGGGAGTC CACCCGGATT TAACAACTCC GAGAGAACAT GCGACAAAGA GTTTATTATA 
CGTAGAACAG CCACCAATCG AGTACTGAAC GTTCTCCGTC ACTGGGTCTC AAAGCACGCA 
CAGGACTTTG AACTCAACAA TGAACTAAAG ATGAACGTCT TAAATTTGCT AGAAGAAGTT 
TTACGAGACC CAGATCTTCT TCCCCAAGAA CGGAAAGCCA CAGCGAACAT CCTAAGGGCC 
CTTTCACAAG ATGATCAAGA TGACATCCAC CTAAAACTAG AGGATATAAT TCAGCTGACG 
GACTGTCCGA AGGCCGAGTG CTTCGAGACC TTGTCGGCCA TGGAGCTGGC TGAGCAGATA 
ACCCTCCTGG ACCACATCGT TTTCAGAAGC ATTCCCTACG AAGAATTTCT TGGGCAGGGG 
TGGATGAAGC TGGATAAAAA TGAAAGAACA CCATACATTA TGAAAACCAG CCAACACTTC 
AATGATATGA GTAACCTGGT GGCCTCCCAG ATCATGAATT ACGCGGATGT CACCTCCCGA 
GCCAACACCA TCGAGAAGTG GGTGGCGGTG GCGGACATCT GCCGGTGCCT GCACAATTAC 
AACGGCGTGC TGGAGATCAC CTCAGCCTTA AACAGAAGTG CCATCTACCG GCTGAAGAAG 
ACCTGGGCCA AGGTGTCCAA GCAGACAAAA GCTCTAATGG ACAAGCTTCA GAAGACTGTT 
TCGTCTGAAG GAAGATTTAA AAACCTCAGA GAAACCCTTA AAAATTGTAA CCCCCCGGCT 
GTTCCTTATC TGGGGATGTA CCTGACAGAC CTGGCGTTCA TTGAAGAAGG AACACCAAAC 
TTTACCGAGG AAGGCCTCGT CAATTTCTCC AAGATGAGAA TGATATCACA CATCATCAGA 
GAGATACGCC AGTTCCAGCA GACTTCCTAT CGAATCGATC ATCAGCCAAA GGTCACTCAG 
TACTTGCTTG ACAAAGCCCT CATCATAGAT GAAGATACGC TGTATGAGCT GTCACTAAAA 
ATTGAACCTC GGCTCCCTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851071.1
CDS	83..3526
Translation	MKWYYFTVLFGHEGQKPLELRCEEEQDGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIECTLVEEPDASDDDSKGSGQVFGHLDFKIVVEPADAAPFTVVLLAPSRQEKAAWTSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLELFFATSQNNRGEHLVEGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSPLNSRIGALDLTTSSASSSPTTTHSPAASPPPHTGKVPLDLSRGLSSPEQSPGSVEESVDNPRVDLCNKLKRSIQRAAVLESAPVDRAGVDSSPVADAAELSPCRSPSTPRHLRYRQPGGQTADNSHCSVSPASAFAIATAAAGHGSPPGFNNSERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDIHLKLEDIIQLTDCPKAECFETLSAMELAEQITLLDHIVFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVTSRANTIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIDHQPKVTQYLLDKALIIDEDTLYELSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_024995303.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995303.1

ORF Insert Sequence:

ATGAAATGGT ATTACTTTAC TGTTCTTTTT GGCCACGAAG GTCAGAAGCC ACTAGAGCTG 
CGCTGTGAGG AGGAGCAGGA TGGTAAAGAG TGGATGGAGG CCATTCACCA AGCCAGTTAT 
GCAGACATTT TGATTGAGAG GGAAGTATTA ATGCAGAAGT ACATTCATCT AGTTCAGATC 
GTAGAGACAG AAAAAATTGC AGCTAACCAA CTCCGGCATC AACTTGAAGA TCAAGACACA 
GAAATCGAAA GGCTTAAATC AGAGATTATC GCTCTTAATA AAACAAAGGA ACGAATGAGA 
CCTTACCAAA GCAATCAAGA AGATGAGGAT CCAGACATCA AGAAGATAAA AAAGGTTCAG 
AGCTTCATGC GAGGGTGGTT ATGTAGAAGG AAATGGAAGA CCATCGTGCA GGATTACATT 
TGTTCTCCTC ACGCCGAAAG CATGAGGAAG AGAAACCAGA TCGTGTTCAC CATGGTGGAG 
GCCGAGTCGG AGTACGTTCA CCAGCTCTAC ATCCTCGTCA ATGGCTTCCT GAGGCCCCTG 
CGCATGGCGG CCAGCTCCAA GAAGCCCCCC ATCAGCCACG ACGACGTCAG CAGTATTTTT 
CTCAACAGTG AAACAATCAT GTTTCTTCAC GAAATATTTC ATCAAGGACT GAAGGCAAGA 
ATAGCCAACT GGCCTACTTT AATTTTAGCT GATCTGTTTG ATATTTTGCT CCCCATGCTG 
AACATTTATC AAGAATTTGT GCGTAATCAC CAGTACAGCC TCCAAGTGCT CGCCAATTGT 
AAGCAAAACA GAGACTTTGA CAAACTCTTA AAACAGTATG AAGCCAACCC TGCCTGTGAG 
GGGAGGATGC TGGAGACGTT CTTGACATAT CCAATGTTTC AGATCCCCAG GTATATCATC 
ACGCTTCATG AACTCCTGGC TCATACACCC CATGAGCACG TGGAGAGAAA AAGTCTGGAG 
TTTGCGAAAT CCAAACTAGA GGAACTGTCC AGGGTCATGC ATGATGAAGT GAGTGACACC 
GAAAACATAA GGAAGAACCT TGCCATAGAA AGAATGATCG TCGAGGGCTG TGATATTTTG 
CTGGACACCA GCCAGACTTT CATCCGCCAA GGTTCTCTTA TTCAAGTACC TTCCGTTGAG 
AGAGGGAAAC TTAGTAAAGT TCGCCTGGGT TCACTGTCTT TGAAAAAGGA AGGAGAAAGA 
CAGTGCTTCT TGTTTACAAA ACACTTTTTA ATTTGTACAA GAAGTTCAGG AGGAAAGCTG 
CATCTGCTCA AGACAGGTGG AGTTCTGTCT CTAATAGAAT GTACCTTGGT TGAGGAGCCC 
GATGCGAGCG ATGATGACTC CAAAGGTTCT GGCCAAGTGT TTGGACACTT GGATTTCAAA 
ATAGTGGTGG AGCCTGCAGA TGCTGCCCCT TTCACTGTGG TCTTGTTAGC ACCTTCCCGC 
CAGGAGAAAG CTGCCTGGAC AAGCGACATC AGTCAGTGTG TGGACAACAT ACGGTGTAAT 
GGTTTAATGA CTATAGTGTT TGAAGAAAAT TCCAAAGTTA CTGTGCCACA TATGATTAAG 
TCTGACGCCC GGCTTCACAA GGACGACACT GACATTTGCT TCAGTAAAAC CCTCAACTCC 
TGCAAAGTGC CGCAGATCCG GTATGCCAGC GTGGAGCGCC TCTTGGAGAG ACTGACCGAC 
TTGCGGTTTC TTAGCATCGA TTTCCTCAAC ACCTTTCTGC ACACCTATCG GATTTTCACG 
ACAGCAGCTG TGGTGCTGGG GAAGCTTTCT GACATATATA AGAGGCCTTT CACCTCCATC 
CCTGTCAGGT CATTGGAACT CTTTTTTGCT ACCAGCCAGA ACAACAGAGG TGAACACTTG 
GTGGAAGGCA AATCCCCACG CCTGTGTCGC AAATTCTCTT CCCCACCTCC ACTGGCTGTG 
TCCAGGACGT CCTCCCCAGT GAGGGCCAGA AAGCTGTCCT TGACTTCTCC CTTGAATTCA 
AGGATAGGCG CACTGGATCT GACCACCTCC TCAGCATCCA GCAGCCCCAC CACCACCCAC 
AGCCCAGCTG CATCCCCACC GCCTCACACT GGTAAAGTGC CGTTGGATCT GAGCAGAGGC 
CTTTCTTCTC CAGAACAAAG CCCGGGATCT GTAGAGGAGT CTGTTGATAA CCCCCGTGTG 
GATCTGTGTA ACAAGCTAAA ACGAAGTATT CAGAGAGCAG CAGTCCTCGA GTCTGCGCCG 
GTGGACCGAG CAGGTGTGGA CAGCTCACCC GTAGCCGATG CCGCAGAACT GTCACCTTGC 
AGGTCACCCT CAACTCCTCG GCACCTCCGC TACCGCCAGC CTGGAGGACA GACGGCTGAC 
AACTCCCACT GCTCTGTTTC ACCGGCCTCT GCTTTTGCAA TCGCTACAGC TGCAGCAGGA 
CACGGGAGTC CACCCGGATT TAACAACTCC GAGAGAACAT GCGACAAAGA GTTTATTATA 
CGTAGAACAG CCACCAATCG AGTACTGAAC GTTCTCCGTC ACTGGGTCTC AAAGCACGCA 
CAGGACTTTG AACTCAACAA TGAACTAAAG ATGAACGTCT TAAATTTGCT AGAAGAAGTT 
TTACGAGACC CAGATCTTCT TCCCCAAGAA CGGAAAGCCA CAGCGAACAT CCTAAGGGCC 
CTTTCACAAG ATGATCAAGA TGACATCCAC CTAAAACTAG AGGATATAAT TCAGCTGACG 
GACTGTCCGA AGGCCGAGTG CTTCGAGACC TTGTCGGCCA TGGAGCTGGC TGAGCAGATA 
ACCCTCCTGG ACCACATCGT TTTCAGAAGC ATTCCCTACG AAGAATTTCT TGGGCAGGGG 
TGGATGAAGC TGGATAAAAA TGAAAGAACA CCATACATTA TGAAAACCAG CCAACACTTC 
AATGATATGA GTAACCTGGT GGCCTCCCAG ATCATGAATT ACGCGGATGT CACCTCCCGA 
GCCAACACCA TCGAGAAGTG GGTGGCGGTG GCGGACATCT GCCGGTGCCT GCACAATTAC 
AACGGCGTGC TGGAGATCAC CTCAGCCTTA AACAGAAGTG CCATCTACCG GCTGAAGAAG 
ACCTGGGCCA AGGTGTCCAA GCAGACAAAA GCTCTAATGG ACAAGCTTCA GAAGACTGTT 
TCGTCTGAAG GAAGATTTAA AAACCTCAGA GAAACCCTTA AAAATTGTAA CCCCCCGGCT 
GTTCCTTATC TGGGGATGTA CCTGACAGAC CTGGCGTTCA TTGAAGAAGG AACACCAAAC 
TTTACCGAGG AAGGCCTCGT CAATTTCTCC AAGATGAGAA TGATATCACA CATCATCAGA 
GAGATACGCC AGTTCCAGCA GACTTCCTAT CGAATCGATC ATCAGCCAAA GGTCACTCAG 
TACTTGCTTG ACAAAGCCCT CATCATAGAT GAAGATACGC TGTATGAGCT GTCACTAAAA 
ATTGAACCTC GGCTCCCTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2 ( XM_015464370.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319856.1 Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), mRNA.

Price & Availability↑

CloneID	OBa90367
Clone ID Related Accession (Same CDS sequence)	XM_015464370.1 , XM_002689373.5
Accession Version	XM_015464370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	ras-specific guanine nucleotide-releasing factor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645156.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCA TTCACCAAGC CAGTTATGCA GACATTTTGA TTGAGAGGGA AGTATTAATG 
CAGAAGTACA TTCATCTAGT TCAGATCGTA GAGACAGAAA AAATTGCAGC TAACCAACTC 
CGGCATCAAC TTGAAGATCA AGACACAGAA ATCGAAAGGC TTAAATCAGA GATTATCGCT 
CTTAATAAAA CAAAGGAACG AATGAGACCT TACCAAAGCA ATCAAGAAGA TGAGGATCCA 
GACATCAAGA AGATAAAAAA GGTTCAGAGC TTCATGAGAG GGTGGTTATG TAGAAGGAAA 
TGGAAGACCA TCGTGCAGGA TTACATTTGT TCTCCTCACG CCGAAAGCAT GAGGAAGAGA 
AACCAGATCG TGTTCACCAT GGTGGAGGCC GAGTCGGAGT ACGTTCACCA GCTCTACATC 
CTCGTCAATG GCTTCCTGAG GCCCCTGCGC ATGGCGGCCA GCTCCAAGAA GCCCCCCATC 
AGCCACGACG ACGTCAGCAG TATTTTTCTC AACAGTGAAA CAATCATGTT TCTTCACGAA 
ATATTTCATC AAGGACTGAA GGCAAGAATA GCCAACTGGC CTACTTTAAT TTTAGCTGAT 
CTGTTTGATA TTTTGCTCCC CATGCTGAAC ATTTATCAAG AATTTGTGCG TAATCACCAG 
TACAGCCTCC AAGTGCTCGC CAATTGTAAG CAAAACAGAG ACTTTGACAA ACTCTTAAAA 
CAGTATGAAG CCAACCCTGC CTGTGAGGGG AGGATGCTGG AGACGTTCTT GACATATCCA 
ATGTTTCAGA TCCCCAGGTA TATCATCACG CTTCATGAAC TCCTGGCTCA TACACCCCAT 
GAGCACGTGG AGAGAAAAAG TCTGGAGTTT GCGAAATCCA AACTAGAGGA ACTGTCCAGG 
GTCATGCATG ATGAAGTGAG TGACACCGAA AACATAAGGA AGAACCTTGC CATAGAAAGA 
ATGATCGTCG AGGGCTGTGA TATTTTGCTG GACACCAGCC AGACTTTCAT CCGCCAAGGT 
TCTCTTATTC AAGTACCTTC CGTTGAGAGA GGGAAACTTA GTAAAGTTCG CCTGGGTTCA 
CTGTCTTTGA AAAAGGAAGG AGAAAGACAG TGCTTCTTGT TTACAAAACA CTTTTTAATT 
TGTACAAGAA GTTCAGGAGG AAAGCTGCAT CTGCTCAAGA CAGGTGGAGT TCTGTCTCTA 
ATAGAATGTA CCTTGGTTGA GGAGCCCGAT GCGAGCGATG ATGACTCCAA AGGTTCTGGC 
CAAGTGTTTG GACACTTGGA TTTCAAAATA GTGGTGGAGC CTGCAGATGC TGCCCCTTTC 
ACTGTGGTCT TGTTAGCACC TTCCCGCCAG GAGAAAGCTG CCTGGACAAG CGACATCAGT 
CAGTGTGTGG ACAACATACG GTGTAATGGT TTAATGACTA TAGTGTTTGA AGAAAATTCC 
AAAGTTACTG TGCCACATAT GATTAAGTCT GACGCCCGGC TTCACAAGGA CGACACTGAC 
ATTTGCTTCA GTAAAACCCT CAACTCCTGC AAAGTGCCGC AGATCCGGTA TGCCAGCGTG 
GAGCGCCTCT TGGAGAGACT GACCGACTTG CGGTTTCTTA GCATCGATTT CCTCAACACC 
TTTCTGCACA CCTATCGGAT TTTCACGACA GCAGCTGTGG TGCTGGGGAA GCTTTCTGAC 
ATATATAAGA GGCCTTTCAC CTCCATCCCT GTCAGGTCAT TGGAACTCTT TTTTGCTACC 
AGCCAGAACA ACAGAGGTGA ACACTTGGTG GAAGGCAAAT CCCCACGCCT GTGTCGCAAA 
TTCTCTTCCC CACCTCCACT GGCTGTGTCC AGGACGTCCT CCCCAGTGAG GGCCAGAAAG 
CTGTCCTTGA CTTCTCCCTT GAATTCAAGG ATAGGCGCAC TGGATCTGAC CACCTCCTCA 
GCATCCAGCA GCCCCACCAC CACCCACAGC CCAGCTGCAT CCCCACCGCC TCACACTGGT 
AAAGTGCCGT TGGATCTGAG CAGAGGCCTT TCTTCTCCAG AACAAAGCCC GGGATCTGTA 
GAGGAGTCTG TTGATAACCC CCGTGTGGAT CTGTGTAACA AGCTAAAACG AAGTATTCAG 
AGAGCAGCAG TCCTCGAGTC TGCGCCGGTG GACCGAGCAG GTGTGGACAG CTCACCCGTA 
GCCGATGCCG CAGAACTGTC ACCTTGCAGG TCACCCTCAA CTCCTCGGCA CCTCCGCTAC 
CGCCAGCCTG GAGGACAGAC GGCTGACAAC TCCCACTGCT CTGTTTCACC GGCCTCTGCT 
TTTGCAATCG CTACAGCTGC AGCAGGACAC GGGAGTCCAC CCGGATTTAA CAACTCCGAG 
AGAACATGCG ACAAAGAGTT TATTATACGT AGAACAGCCA CCAATCGAGT ACTGAACGTT 
CTCCGTCACT GGGTCTCAAA GCACGCACAG GACTTTGAAC TCAACAATGA ACTAAAGATG 
AACGTCTTAA ATTTGCTAGA AGAAGTTTTA CGAGACCCAG ATCTTCTTCC CCAAGAACGG 
AAAGCCACAG CGAACATCCT AAGGGCCCTT TCACAAGATG ATCAAGATGA CATCCACCTA 
AAACTAGAGG ATATAATTCA GCTGACGGAC TGTCCGAAGG CCGAGTGCTT CGAGACCTTG 
TCGGCCATGG AGCTGGCTGA GCAGATAACC CTCCTGGACC ACATCGTTTT CAGAAGCATT 
CCCTACGAAG AATTTCTTGG GCAGGGGTGG ATGAAGCTGG ATAAAAATGA AAGAACACCA 
TACATTATGA AAACCAGCCA ACACTTCAAT GATATGAGTA ACCTGGTGGC CTCCCAGATC 
ATGAATTACG CGGATGTCAC CTCCCGAGCC AACACCATCG AGAAGTGGGT GGCGGTGGCG 
GACATCTGCC GGTGCCTGCA CAATTACAAC GGCGTGCTGG AGATCACCTC AGCCTTAAAC 
AGAAGTGCCA TCTACCGGCT GAAGAAGACC TGGGCCAAGG TGTCCAAGCA GACAAAAGCT 
CTAATGGACA AGCTTCAGAA GACTGTTTCG TCTGAAGGAA GATTTAAAAA CCTCAGAGAA 
ACCCTTAAAA ATTGTAACCC CCCGGCTGTT CCTTATCTGG GGATGTACCT GACAGACCTG 
GCGTTCATTG AAGAAGGAAC ACCAAACTTT ACCGAGGAAG GCCTCGTCAA TTTCTCCAAG 
ATGAGAATGA TATCACACAT CATCAGAGAG ATACGCCAGT TCCAGCAGAC TTCCTATCGA 
ATCGATCATC AGCCAAAGGT CACTCAGTAC TTGCTTGACA AAGCCCTCAT CATAGATGAA 
GATACGCTGT ATGAGCTGTC ACTAAAAATT GAACCTCGGC TCCCTGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319856.1
CDS	108..3458
Translation	MEAIHQASYADILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIECTLVEEPDASDDDSKGSGQVFGHLDFKIVVEPADAAPFTVVLLAPSRQEKAAWTSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLELFFATSQNNRGEHLVEGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSPLNSRIGALDLTTSSASSSPTTTHSPAASPPPHTGKVPLDLSRGLSSPEQSPGSVEESVDNPRVDLCNKLKRSIQRAAVLESAPVDRAGVDSSPVADAAELSPCRSPSTPRHLRYRQPGGQTADNSHCSVSPASAFAIATAAAGHGSPPGFNNSERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDIHLKLEDIIQLTDCPKAECFETLSAMELAEQITLLDHIVFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVTSRANTIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIDHQPKVTQYLLDKALIIDEDTLYELSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_015464370.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464370.1

ORF Insert Sequence:

ATGGAGGCCA TTCACCAAGC CAGTTATGCA GACATTTTGA TTGAGAGGGA AGTATTAATG 
CAGAAGTACA TTCATCTAGT TCAGATCGTA GAGACAGAAA AAATTGCAGC TAACCAACTC 
CGGCATCAAC TTGAAGATCA AGACACAGAA ATCGAAAGGC TTAAATCAGA GATTATCGCT 
CTTAATAAAA CAAAGGAACG AATGAGACCT TACCAAAGCA ATCAAGAAGA TGAGGATCCA 
GACATCAAGA AGATAAAAAA GGTTCAGAGC TTCATGAGAG GGTGGTTATG TAGAAGGAAA 
TGGAAGACCA TCGTGCAGGA TTACATTTGT TCTCCTCACG CCGAAAGCAT GAGGAAGAGA 
AACCAGATCG TGTTCACCAT GGTGGAGGCC GAGTCGGAGT ACGTTCACCA GCTCTACATC 
CTCGTCAATG GCTTCCTGAG GCCCCTGCGC ATGGCGGCCA GCTCCAAGAA GCCCCCCATC 
AGCCACGACG ACGTCAGCAG TATTTTTCTC AACAGTGAAA CAATCATGTT TCTTCACGAA 
ATATTTCATC AAGGACTGAA GGCAAGAATA GCCAACTGGC CTACTTTAAT TTTAGCTGAT 
CTGTTTGATA TTTTGCTCCC CATGCTGAAC ATTTATCAAG AATTTGTGCG TAATCACCAG 
TACAGCCTCC AAGTGCTCGC CAATTGTAAG CAAAACAGAG ACTTTGACAA ACTCTTAAAA 
CAGTATGAAG CCAACCCTGC CTGTGAGGGG AGGATGCTGG AGACGTTCTT GACATATCCA 
ATGTTTCAGA TCCCCAGGTA TATCATCACG CTTCATGAAC TCCTGGCTCA TACACCCCAT 
GAGCACGTGG AGAGAAAAAG TCTGGAGTTT GCGAAATCCA AACTAGAGGA ACTGTCCAGG 
GTCATGCATG ATGAAGTGAG TGACACCGAA AACATAAGGA AGAACCTTGC CATAGAAAGA 
ATGATCGTCG AGGGCTGTGA TATTTTGCTG GACACCAGCC AGACTTTCAT CCGCCAAGGT 
TCTCTTATTC AAGTACCTTC CGTTGAGAGA GGGAAACTTA GTAAAGTTCG CCTGGGTTCA 
CTGTCTTTGA AAAAGGAAGG AGAAAGACAG TGCTTCTTGT TTACAAAACA CTTTTTAATT 
TGTACAAGAA GTTCAGGAGG AAAGCTGCAT CTGCTCAAGA CAGGTGGAGT TCTGTCTCTA 
ATAGAATGTA CCTTGGTTGA GGAGCCCGAT GCGAGCGATG ATGACTCCAA AGGTTCTGGC 
CAAGTGTTTG GACACTTGGA TTTCAAAATA GTGGTGGAGC CTGCAGATGC TGCCCCTTTC 
ACTGTGGTCT TGTTAGCACC TTCCCGCCAG GAGAAAGCTG CCTGGACAAG CGACATCAGT 
CAGTGTGTGG ACAACATACG GTGTAATGGT TTAATGACTA TAGTGTTTGA AGAAAATTCC 
AAAGTTACTG TGCCACATAT GATTAAGTCT GACGCCCGGC TTCACAAGGA CGACACTGAC 
ATTTGCTTCA GTAAAACCCT CAACTCCTGC AAAGTGCCGC AGATCCGGTA TGCCAGCGTG 
GAGCGCCTCT TGGAGAGACT GACCGACTTG CGGTTTCTTA GCATCGATTT CCTCAACACC 
TTTCTGCACA CCTATCGGAT TTTCACGACA GCAGCTGTGG TGCTGGGGAA GCTTTCTGAC 
ATATATAAGA GGCCTTTCAC CTCCATCCCT GTCAGGTCAT TGGAACTCTT TTTTGCTACC 
AGCCAGAACA ACAGAGGTGA ACACTTGGTG GAAGGCAAAT CCCCACGCCT GTGTCGCAAA 
TTCTCTTCCC CACCTCCACT GGCTGTGTCC AGGACGTCCT CCCCAGTGAG GGCCAGAAAG 
CTGTCCTTGA CTTCTCCCTT GAATTCAAGG ATAGGCGCAC TGGATCTGAC CACCTCCTCA 
GCATCCAGCA GCCCCACCAC CACCCACAGC CCAGCTGCAT CCCCACCGCC TCACACTGGT 
AAAGTGCCGT TGGATCTGAG CAGAGGCCTT TCTTCTCCAG AACAAAGCCC GGGATCTGTA 
GAGGAGTCTG TTGATAACCC CCGTGTGGAT CTGTGTAACA AGCTAAAACG AAGTATTCAG 
AGAGCAGCAG TCCTCGAGTC TGCGCCGGTG GACCGAGCAG GTGTGGACAG CTCACCCGTA 
GCCGATGCCG CAGAACTGTC ACCTTGCAGG TCACCCTCAA CTCCTCGGCA CCTCCGCTAC 
CGCCAGCCTG GAGGACAGAC GGCTGACAAC TCCCACTGCT CTGTTTCACC GGCCTCTGCT 
TTTGCAATCG CTACAGCTGC AGCAGGACAC GGGAGTCCAC CCGGATTTAA CAACTCCGAG 
AGAACATGCG ACAAAGAGTT TATTATACGT AGAACAGCCA CCAATCGAGT ACTGAACGTT 
CTCCGTCACT GGGTCTCAAA GCACGCACAG GACTTTGAAC TCAACAATGA ACTAAAGATG 
AACGTCTTAA ATTTGCTAGA AGAAGTTTTA CGAGACCCAG ATCTTCTTCC CCAAGAACGG 
AAAGCCACAG CGAACATCCT AAGGGCCCTT TCACAAGATG ATCAAGATGA CATCCACCTA 
AAACTAGAGG ATATAATTCA GCTGACGGAC TGTCCGAAGG CCGAGTGCTT CGAGACCTTG 
TCGGCCATGG AGCTGGCTGA GCAGATAACC CTCCTGGACC ACATCGTTTT CAGAAGCATT 
CCCTACGAAG AATTTCTTGG GCAGGGGTGG ATGAAGCTGG ATAAAAATGA AAGAACACCA 
TACATTATGA AAACCAGCCA ACACTTCAAT GATATGAGTA ACCTGGTGGC CTCCCAGATC 
ATGAATTACG CGGATGTCAC CTCCCGAGCC AACACCATCG AGAAGTGGGT GGCGGTGGCG 
GACATCTGCC GGTGCCTGCA CAATTACAAC GGCGTGCTGG AGATCACCTC AGCCTTAAAC 
AGAAGTGCCA TCTACCGGCT GAAGAAGACC TGGGCCAAGG TGTCCAAGCA GACAAAAGCT 
CTAATGGACA AGCTTCAGAA GACTGTTTCG TCTGAAGGAA GATTTAAAAA CCTCAGAGAA 
ACCCTTAAAA ATTGTAACCC CCCGGCTGTT CCTTATCTGG GGATGTACCT GACAGACCTG 
GCGTTCATTG AAGAAGGAAC ACCAAACTTT ACCGAGGAAG GCCTCGTCAA TTTCTCCAAG 
ATGAGAATGA TATCACACAT CATCAGAGAG ATACGCCAGT TCCAGCAGAC TTCCTATCGA 
ATCGATCATC AGCCAAAGGT CACTCAGTAC TTGCTTGACA AAGCCCTCAT CATAGATGAA 
GATACGCTGT ATGAGCTGTC ACTAAAAATT GAACCTCGGC TCCCTGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2 ( XM_002689373.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002689419.3 Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), mRNA.

Price & Availability↑

CloneID	OBa90367
Clone ID Related Accession (Same CDS sequence)	XM_015464370.1 , XM_002689373.5
Accession Version	XM_002689373.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	ras-specific guanine nucleotide-releasing factor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002689373.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCA TTCACCAAGC CAGTTATGCA GACATTTTGA TTGAGAGGGA AGTATTAATG 
CAGAAGTACA TTCATCTAGT TCAGATCGTA GAGACAGAAA AAATTGCAGC TAACCAACTC 
CGGCATCAAC TTGAAGATCA AGACACAGAA ATCGAAAGGC TTAAATCAGA GATTATCGCT 
CTTAATAAAA CAAAGGAACG AATGAGACCT TACCAAAGCA ATCAAGAAGA TGAGGATCCA 
GACATCAAGA AGATAAAAAA GGTTCAGAGC TTCATGAGAG GGTGGTTATG TAGAAGGAAA 
TGGAAGACCA TCGTGCAGGA TTACATTTGT TCTCCTCACG CCGAAAGCAT GAGGAAGAGA 
AACCAGATCG TGTTCACCAT GGTGGAGGCC GAGTCGGAGT ACGTTCACCA GCTCTACATC 
CTCGTCAATG GCTTCCTGAG GCCCCTGCGC ATGGCGGCCA GCTCCAAGAA GCCCCCCATC 
AGCCACGACG ACGTCAGCAG TATTTTTCTC AACAGTGAAA CAATCATGTT TCTTCACGAA 
ATATTTCATC AAGGACTGAA GGCAAGAATA GCCAACTGGC CTACTTTAAT TTTAGCTGAT 
CTGTTTGATA TTTTGCTCCC CATGCTGAAC ATTTATCAAG AATTTGTGCG TAATCACCAG 
TACAGCCTCC AAGTGCTCGC CAATTGTAAG CAAAACAGAG ACTTTGACAA ACTCTTAAAA 
CAGTATGAAG CCAACCCTGC CTGTGAGGGG AGGATGCTGG AGACGTTCTT GACATATCCA 
ATGTTTCAGA TCCCCAGGTA TATCATCACG CTTCATGAAC TCCTGGCTCA TACACCCCAT 
GAGCACGTGG AGAGAAAAAG TCTGGAGTTT GCGAAATCCA AACTAGAGGA ACTGTCCAGG 
GTCATGCATG ATGAAGTGAG TGACACCGAA AACATAAGGA AGAACCTTGC CATAGAAAGA 
ATGATCGTCG AGGGCTGTGA TATTTTGCTG GACACCAGCC AGACTTTCAT CCGCCAAGGT 
TCTCTTATTC AAGTACCTTC CGTTGAGAGA GGGAAACTTA GTAAAGTTCG CCTGGGTTCA 
CTGTCTTTGA AAAAGGAAGG AGAAAGACAG TGCTTCTTGT TTACAAAACA CTTTTTAATT 
TGTACAAGAA GTTCAGGAGG AAAGCTGCAT CTGCTCAAGA CAGGTGGAGT TCTGTCTCTA 
ATAGAATGTA CCTTGGTTGA GGAGCCCGAT GCGAGCGATG ATGACTCCAA AGGTTCTGGC 
CAAGTGTTTG GACACTTGGA TTTCAAAATA GTGGTGGAGC CTGCAGATGC TGCCCCTTTC 
ACTGTGGTCT TGTTAGCACC TTCCCGCCAG GAGAAAGCTG CCTGGACAAG CGACATCAGT 
CAGTGTGTGG ACAACATACG GTGTAATGGT TTAATGACTA TAGTGTTTGA AGAAAATTCC 
AAAGTTACTG TGCCACATAT GATTAAGTCT GACGCCCGGC TTCACAAGGA CGACACTGAC 
ATTTGCTTCA GTAAAACCCT CAACTCCTGC AAAGTGCCGC AGATCCGGTA TGCCAGCGTG 
GAGCGCCTCT TGGAGAGACT GACCGACTTG CGGTTTCTTA GCATCGATTT CCTCAACACC 
TTTCTGCACA CCTATCGGAT TTTCACGACA GCAGCTGTGG TGCTGGGGAA GCTTTCTGAC 
ATATATAAGA GGCCTTTCAC CTCCATCCCT GTCAGGTCAT TGGAACTCTT TTTTGCTACC 
AGCCAGAACA ACAGAGGTGA ACACTTGGTG GAAGGCAAAT CCCCACGCCT GTGTCGCAAA 
TTCTCTTCCC CACCTCCACT GGCTGTGTCC AGGACGTCCT CCCCAGTGAG GGCCAGAAAG 
CTGTCCTTGA CTTCTCCCTT GAATTCAAGG ATAGGCGCAC TGGATCTGAC CACCTCCTCA 
GCATCCAGCA GCCCCACCAC CACCCACAGC CCAGCTGCAT CCCCACCGCC TCACACTGGT 
AAAGTGCCGT TGGATCTGAG CAGAGGCCTT TCTTCTCCAG AACAAAGCCC GGGATCTGTA 
GAGGAGTCTG TTGATAACCC CCGTGTGGAT CTGTGTAACA AGCTAAAACG AAGTATTCAG 
AGAGCAGCAG TCCTCGAGTC TGCGCCGGTG GACCGAGCAG GTGTGGACAG CTCACCCGTA 
GCCGATGCCG CAGAACTGTC ACCTTGCAGG TCACCCTCAA CTCCTCGGCA CCTCCGCTAC 
CGCCAGCCTG GAGGACAGAC GGCTGACAAC TCCCACTGCT CTGTTTCACC GGCCTCTGCT 
TTTGCAATCG CTACAGCTGC AGCAGGACAC GGGAGTCCAC CCGGATTTAA CAACTCCGAG 
AGAACATGCG ACAAAGAGTT TATTATACGT AGAACAGCCA CCAATCGAGT ACTGAACGTT 
CTCCGTCACT GGGTCTCAAA GCACGCACAG GACTTTGAAC TCAACAATGA ACTAAAGATG 
AACGTCTTAA ATTTGCTAGA AGAAGTTTTA CGAGACCCAG ATCTTCTTCC CCAAGAACGG 
AAAGCCACAG CGAACATCCT AAGGGCCCTT TCACAAGATG ATCAAGATGA CATCCACCTA 
AAACTAGAGG ATATAATTCA GCTGACGGAC TGTCCGAAGG CCGAGTGCTT CGAGACCTTG 
TCGGCCATGG AGCTGGCTGA GCAGATAACC CTCCTGGACC ACATCGTTTT CAGAAGCATT 
CCCTACGAAG AATTTCTTGG GCAGGGGTGG ATGAAGCTGG ATAAAAATGA AAGAACACCA 
TACATTATGA AAACCAGCCA ACACTTCAAT GATATGAGTA ACCTGGTGGC CTCCCAGATC 
ATGAATTACG CGGATGTCAC CTCCCGAGCC AACACCATCG AGAAGTGGGT GGCGGTGGCG 
GACATCTGCC GGTGCCTGCA CAATTACAAC GGCGTGCTGG AGATCACCTC AGCCTTAAAC 
AGAAGTGCCA TCTACCGGCT GAAGAAGACC TGGGCCAAGG TGTCCAAGCA GACAAAAGCT 
CTAATGGACA AGCTTCAGAA GACTGTTTCG TCTGAAGGAA GATTTAAAAA CCTCAGAGAA 
ACCCTTAAAA ATTGTAACCC CCCGGCTGTT CCTTATCTGG GGATGTACCT GACAGACCTG 
GCGTTCATTG AAGAAGGAAC ACCAAACTTT ACCGAGGAAG GCCTCGTCAA TTTCTCCAAG 
ATGAGAATGA TATCACACAT CATCAGAGAG ATACGCCAGT TCCAGCAGAC TTCCTATCGA 
ATCGATCATC AGCCAAAGGT CACTCAGTAC TTGCTTGACA AAGCCCTCAT CATAGATGAA 
GATACGCTGT ATGAGCTGTC ACTAAAAATT GAACCTCGGC TCCCTGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002689419.3
CDS	108..3458
Translation	MEAIHQASYADILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIECTLVEEPDASDDDSKGSGQVFGHLDFKIVVEPADAAPFTVVLLAPSRQEKAAWTSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLELFFATSQNNRGEHLVEGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSPLNSRIGALDLTTSSASSSPTTTHSPAASPPPHTGKVPLDLSRGLSSPEQSPGSVEESVDNPRVDLCNKLKRSIQRAAVLESAPVDRAGVDSSPVADAAELSPCRSPSTPRHLRYRQPGGQTADNSHCSVSPASAFAIATAAAGHGSPPGFNNSERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRALSQDDQDDIHLKLEDIIQLTDCPKAECFETLSAMELAEQITLLDHIVFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVTSRANTIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIDHQPKVTQYLLDKALIIDEDTLYELSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_002689373.5
Organism	Bos taurus(cattle)
Definition	Bos taurus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002689373.5

ORF Insert Sequence:

ATGGAGGCCA TTCACCAAGC CAGTTATGCA GACATTTTGA TTGAGAGGGA AGTATTAATG 
CAGAAGTACA TTCATCTAGT TCAGATCGTA GAGACAGAAA AAATTGCAGC TAACCAACTC 
CGGCATCAAC TTGAAGATCA AGACACAGAA ATCGAAAGGC TTAAATCAGA GATTATCGCT 
CTTAATAAAA CAAAGGAACG AATGAGACCT TACCAAAGCA ATCAAGAAGA TGAGGATCCA 
GACATCAAGA AGATAAAAAA GGTTCAGAGC TTCATGAGAG GGTGGTTATG TAGAAGGAAA 
TGGAAGACCA TCGTGCAGGA TTACATTTGT TCTCCTCACG CCGAAAGCAT GAGGAAGAGA 
AACCAGATCG TGTTCACCAT GGTGGAGGCC GAGTCGGAGT ACGTTCACCA GCTCTACATC 
CTCGTCAATG GCTTCCTGAG GCCCCTGCGC ATGGCGGCCA GCTCCAAGAA GCCCCCCATC 
AGCCACGACG ACGTCAGCAG TATTTTTCTC AACAGTGAAA CAATCATGTT TCTTCACGAA 
ATATTTCATC AAGGACTGAA GGCAAGAATA GCCAACTGGC CTACTTTAAT TTTAGCTGAT 
CTGTTTGATA TTTTGCTCCC CATGCTGAAC ATTTATCAAG AATTTGTGCG TAATCACCAG 
TACAGCCTCC AAGTGCTCGC CAATTGTAAG CAAAACAGAG ACTTTGACAA ACTCTTAAAA 
CAGTATGAAG CCAACCCTGC CTGTGAGGGG AGGATGCTGG AGACGTTCTT GACATATCCA 
ATGTTTCAGA TCCCCAGGTA TATCATCACG CTTCATGAAC TCCTGGCTCA TACACCCCAT 
GAGCACGTGG AGAGAAAAAG TCTGGAGTTT GCGAAATCCA AACTAGAGGA ACTGTCCAGG 
GTCATGCATG ATGAAGTGAG TGACACCGAA AACATAAGGA AGAACCTTGC CATAGAAAGA 
ATGATCGTCG AGGGCTGTGA TATTTTGCTG GACACCAGCC AGACTTTCAT CCGCCAAGGT 
TCTCTTATTC AAGTACCTTC CGTTGAGAGA GGGAAACTTA GTAAAGTTCG CCTGGGTTCA 
CTGTCTTTGA AAAAGGAAGG AGAAAGACAG TGCTTCTTGT TTACAAAACA CTTTTTAATT 
TGTACAAGAA GTTCAGGAGG AAAGCTGCAT CTGCTCAAGA CAGGTGGAGT TCTGTCTCTA 
ATAGAATGTA CCTTGGTTGA GGAGCCCGAT GCGAGCGATG ATGACTCCAA AGGTTCTGGC 
CAAGTGTTTG GACACTTGGA TTTCAAAATA GTGGTGGAGC CTGCAGATGC TGCCCCTTTC 
ACTGTGGTCT TGTTAGCACC TTCCCGCCAG GAGAAAGCTG CCTGGACAAG CGACATCAGT 
CAGTGTGTGG ACAACATACG GTGTAATGGT TTAATGACTA TAGTGTTTGA AGAAAATTCC 
AAAGTTACTG TGCCACATAT GATTAAGTCT GACGCCCGGC TTCACAAGGA CGACACTGAC 
ATTTGCTTCA GTAAAACCCT CAACTCCTGC AAAGTGCCGC AGATCCGGTA TGCCAGCGTG 
GAGCGCCTCT TGGAGAGACT GACCGACTTG CGGTTTCTTA GCATCGATTT CCTCAACACC 
TTTCTGCACA CCTATCGGAT TTTCACGACA GCAGCTGTGG TGCTGGGGAA GCTTTCTGAC 
ATATATAAGA GGCCTTTCAC CTCCATCCCT GTCAGGTCAT TGGAACTCTT TTTTGCTACC 
AGCCAGAACA ACAGAGGTGA ACACTTGGTG GAAGGCAAAT CCCCACGCCT GTGTCGCAAA 
TTCTCTTCCC CACCTCCACT GGCTGTGTCC AGGACGTCCT CCCCAGTGAG GGCCAGAAAG 
CTGTCCTTGA CTTCTCCCTT GAATTCAAGG ATAGGCGCAC TGGATCTGAC CACCTCCTCA 
GCATCCAGCA GCCCCACCAC CACCCACAGC CCAGCTGCAT CCCCACCGCC TCACACTGGT 
AAAGTGCCGT TGGATCTGAG CAGAGGCCTT TCTTCTCCAG AACAAAGCCC GGGATCTGTA 
GAGGAGTCTG TTGATAACCC CCGTGTGGAT CTGTGTAACA AGCTAAAACG AAGTATTCAG 
AGAGCAGCAG TCCTCGAGTC TGCGCCGGTG GACCGAGCAG GTGTGGACAG CTCACCCGTA 
GCCGATGCCG CAGAACTGTC ACCTTGCAGG TCACCCTCAA CTCCTCGGCA CCTCCGCTAC 
CGCCAGCCTG GAGGACAGAC GGCTGACAAC TCCCACTGCT CTGTTTCACC GGCCTCTGCT 
TTTGCAATCG CTACAGCTGC AGCAGGACAC GGGAGTCCAC CCGGATTTAA CAACTCCGAG 
AGAACATGCG ACAAAGAGTT TATTATACGT AGAACAGCCA CCAATCGAGT ACTGAACGTT 
CTCCGTCACT GGGTCTCAAA GCACGCACAG GACTTTGAAC TCAACAATGA ACTAAAGATG 
AACGTCTTAA ATTTGCTAGA AGAAGTTTTA CGAGACCCAG ATCTTCTTCC CCAAGAACGG 
AAAGCCACAG CGAACATCCT AAGGGCCCTT TCACAAGATG ATCAAGATGA CATCCACCTA 
AAACTAGAGG ATATAATTCA GCTGACGGAC TGTCCGAAGG CCGAGTGCTT CGAGACCTTG 
TCGGCCATGG AGCTGGCTGA GCAGATAACC CTCCTGGACC ACATCGTTTT CAGAAGCATT 
CCCTACGAAG AATTTCTTGG GCAGGGGTGG ATGAAGCTGG ATAAAAATGA AAGAACACCA 
TACATTATGA AAACCAGCCA ACACTTCAAT GATATGAGTA ACCTGGTGGC CTCCCAGATC 
ATGAATTACG CGGATGTCAC CTCCCGAGCC AACACCATCG AGAAGTGGGT GGCGGTGGCG 
GACATCTGCC GGTGCCTGCA CAATTACAAC GGCGTGCTGG AGATCACCTC AGCCTTAAAC 
AGAAGTGCCA TCTACCGGCT GAAGAAGACC TGGGCCAAGG TGTCCAAGCA GACAAAAGCT 
CTAATGGACA AGCTTCAGAA GACTGTTTCG TCTGAAGGAA GATTTAAAAA CCTCAGAGAA 
ACCCTTAAAA ATTGTAACCC CCCGGCTGTT CCTTATCTGG GGATGTACCT GACAGACCTG 
GCGTTCATTG AAGAAGGAAC ACCAAACTTT ACCGAGGAAG GCCTCGTCAA TTTCTCCAAG 
ATGAGAATGA TATCACACAT CATCAGAGAG ATACGCCAGT TCCAGCAGAC TTCCTATCGA 
ATCGATCATC AGCCAAAGGT CACTCAGTAC TTGCTTGACA AAGCCCTCAT CATAGATGAA 
GATACGCTGT ATGAGCTGTC ACTAAAAATT GAACCTCGGC TCCCTGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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RASGRF2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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