Tmlhe cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmlhe
Entrez Gene ID	192289
Full Name	trimethyllysine hydroxylase, epsilon
Synonyms	Bbox2,D430017M14Rik,TMLH
General protein information	Preferred Names trimethyllysine hydroxylase, epsilon Names trimethyllysine dioxygenase, mitochondrial TML dioxygenase TML hydroxylase TML-alpha-ketoglutarate dioxygenase TMLD butyrobetaine (gamma), 2-oxoglutarate dioxygenase 2 (gamma-butyrobetaine hydroxylase) epsilon-trimethyllysine 2-oxoglutarate dioxygenase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X

mRNA and Protein(s)

mRNA	Protein	Name
XM_006535854.3	XP_006535917.1	trimethyllysine dioxygenase, mitochondrial isoform X1
NM_138758.1	NP_620097.1	trimethyllysine dioxygenase, mitochondrial
XM_006535854.4	XP_006535917.1	trimethyllysine dioxygenase, mitochondrial isoform X1
NM_138758.2	NP_620097.1	trimethyllysine dioxygenase, mitochondrial precursor

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_07454	XP_003711352.1
Neurospora crassa	NCU03802	XP_961191.2
Homo sapiens (human)	TMLHE	NP_060666.1
Xenopus tropicalis (tropical clawed frog)	tmlhe	NP_001096300.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005447	XP_315450.4
Eremothecium gossypii	AGOS_AEL035W	NP_984826.2
Kluyveromyces lactis	KLLA0A10945g	XP_451476.1
Pan troglodytes (chimpanzee)	TMLHE	XP_521349.3
Bos taurus (cattle)	TMLHE	NP_001069532.1
Rattus norvegicus (Norway rat)	Tmlhe	NP_596878.2
Danio rerio (zebrafish)	tmlhe	XP_682831.3
Caenorhabditis elegans (roundworm)	gbh-2	NP_495793.2
Macaca mulatta (Rhesus monkey)	TMLHE	XP_001099674.1
Canis lupus familiaris (dog)	TMLHE	XP_853649.1
Mus musculus (house mouse)	Tmlhe	NP_620097.1
Gallus gallus (chicken)	TMLHE	NP_001012593.1
Drosophila melanogaster (fruit fly)	CG4335	NP_650886.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional characterization of the TMLH gene: promoter analysis, in situ hybridization, identification and mapping of alternative splicing variants.
Monfregola J, Napolitano G, Conte I, Cevenini A, Migliaccio C, D'Urso M, Ursini MV
Gene395(1-2)86-97(2007 Jun)

Functional analysis of TMLH variants and definition of domains required for catalytic activity and mitochondrial targeting.
Monfregola J, Cevenini A, Terracciano A, van Vlies N, Arbucci S, Wanders RJ, D'Urso M, Vaz FM, Ursini MV
Journal of cellular physiology204(3)839-47(2005 Sep)

Structural basis of allotypes of ecto-nucleotide pyrophosphatase/phosphodiesterase (plasma cell membrane glycoprotein PC-1) in the mouse and rat, and analysis of allele-specific xenogeneic antibodies.
Banakh I, Sali A, Dubljevic V, Grobben B, Slegers H, Goding JW
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics29(4)307-13(2002 Aug)

Molecular and Biochemical Characterization of Rat epsilon -N-Trimethyllysine Hydroxylase, the First Enzyme of Carnitine Biosynthesis.
Vaz FM, Ofman R, Westinga K, Back JW, Wanders RJ
The Journal of biological chemistry276(36)33512-7(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tmlhe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmlhe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14505	NM_138758.2 Latest version!	Mus musculus trimethyllysine hydroxylase, epsilon (Tmlhe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu57230	XM_006535854.4 Latest version!	Mus musculus trimethyllysine hydroxylase, epsilon (Tmlhe), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu57230	XM_006535854.3	Mus musculus trimethyllysine hydroxylase, epsilon (Tmlhe), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu14505	NM_138758.1	Mus musculus trimethyllysine hydroxylase, epsilon (Tmlhe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmlhe ( NM_138758.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_620097.1 Mus musculus trimethyllysine hydroxylase, epsilon (Tmlhe), mRNA.

Price & Availability↑

CloneID	OMu14505
Clone ID Related Accession (Same CDS sequence)	NM_138758.1 , NM_138758.2
Accession Version	NM_138758.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Mus musculus(house mouse)
Product	trimethyllysine dioxygenase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC010495.1. On Dec 7, 2005 this sequence version replaced XM_895293.1. ##Evidence-Data-START## Transcript exon combination :: BC010495.1, AK144627.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTGGTACC ATAAGTTGTT GCACCAACAA AGCAGGCTCC GTAATTTGAT GAAGAGAGGA 
AACATAGCTC AAGGTCTTCA TCTGTCCAAC TTTAAAAGTT TATTTTCATC ATCCATCCAT 
TGGTGCCATA CAACTTCCAA GTCTGTGAAC TGTACTTGGC ACCAACATGA AGATCATTTG 
GAACTGCAAT ATGCTGGCAC TGTGATGCGC TTTGATTATG TCTGGCTTCG AGACCATTGC 
CGCTCAGCAT CATGCTATAA TTCTAAGACT CACCAGCGGA GCCTGGACAC TGCCAGTGTG 
GATTTATGTA TTAAGCCAAA GACAGTTCAT CTGGATGAAA CTATGCTCTT TTTCACTTGG 
CCAGATGGTC ATGTGACTAG ATATGATCTG GATTGGCTGG TGAAAAACAG TTATGAAGGG 
CAGAAACAAA AGGTTATCCA ACCTAGAATA TTATGGAATT CTAAACTCTA CCAGCAAGCC 
CAAGTTCCAT CTGTTGATTT CCAGTGCTTT TTAGAAACAA ATGAAGGGTT GAAGAAGTTT 
CTGCAGAACT TTTTGCTGTA TGGAATTGCA TTTGTAGAAA ATGTCCCTCC CACTGAAGAG 
CATACAGAAA AATTGGCAGA AAGGATTAGC TTAATCAGAG AAACCATCTA TGGAAGGATG 
TGGTATTTCA CTTCAGATTT TTCCAGAGGT GACACTGCAT ACACCAAGCT AGCTCTAGAC 
CGGCATACTG ACACTACCTA CTTTCAAGAG CCCTGTGGCA TTCAAGTATT TCATTGTCTT 
AAGCATGAAG GAACAGGTGG TAGAACACTG TTAGTGGATG GATTCTATGC AGCACAACAA 
GTACTTCAAA AAGCACCTGA GGAATTTGAA CTCCTCAGCA AAGTACCATT GAAGCATGAA 
TATATTGAAA ATGTTGGACA ATGTCACAAT CACATGATTG GGGTTGGGCC CATCTTAAAT 
ATTTACCCAT GGAATAAAGA GCTGTATTTG ATCAGGTACA ACAACTATGA CAGGGCTGTC 
ATCAACACTG TGCCTTATGA TGTTGTCCAT CGTTGGTATA CAGCACACCG AACCCTAACA 
ACTGAATTGA GAAGGCCTGA GAATGAGCTT TGGGTCAAAT TGAAGCCTGG AAAGGTACTA 
TTTATAGACA ACTGGCGAGT ACTTCATGGC AGGGAATCAT TCACTGGCTA CCGTCAACTC 
TGTGGCTGCT ATTTAACTAG AGATGATGTA TTGAATACTG CTCGTCTCCT GGGTCTTCAT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620097.1
CDS	129..1394
Translation	MWYHKLLHQQSRLRNLMKRGNIAQGLHLSNFKSLFSSSIHWCHTTSKSVNCTWHQHEDHLELQYAGTVMRFDYVWLRDHCRSASCYNSKTHQRSLDTASVDLCIKPKTVHLDETMLFFTWPDGHVTRYDLDWLVKNSYEGQKQKVIQPRILWNSKLYQQAQVPSVDFQCFLETNEGLKKFLQNFLLYGIAFVENVPPTEEHTEKLAERISLIRETIYGRMWYFTSDFSRGDTAYTKLALDRHTDTTYFQEPCGIQVFHCLKHEGTGGRTLLVDGFYAAQQVLQKAPEEFELLSKVPLKHEYIENVGQCHNHMIGVGPILNIYPWNKELYLIRYNNYDRAVINTVPYDVVHRWYTAHRTLTTELRRPENELWVKLKPGKVLFIDNWRVLHGRESFTGYRQLCGCYLTRDDVLNTARLLGLHA

Target ORF information:

RefSeq Version	NM_138758.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus trimethyllysine hydroxylase, epsilon (Tmlhe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138758.1

ORF Insert Sequence:

ATGTGGTACC ATAAGTTGTT GCACCAACAA AGCAGGCTCC GTAATTTGAT GAAGAGAGGA 
AACATAGCTC AAGGTCTTCA TCTGTCCAAC TTTAAAAGTT TATTTTCATC ATCCATCCAT 
TGGTGCCATA CAACTTCCAA GTCTGTGAAC TGTACTTGGC ACCAACATGA AGATCATTTG 
GAACTGCAAT ATGCTGGCAC TGTGATGCGC TTTGATTATG TCTGGCTTCG AGACCATTGC 
CGCTCAGCAT CATGCTATAA TTCTAAGACT CACCAGCGGA GCCTGGACAC TGCCAGTGTG 
GATTTATGTA TTAAGCCAAA GACAGTTCAT CTGGATGAAA CTATGCTCTT TTTCACTTGG 
CCAGATGGTC ATGTGACTAG ATATGATCTG GATTGGCTGG TGAAAAACAG TTATGAAGGG 
CAGAAACAAA AGGTTATCCA ACCTAGAATA TTATGGAATT CTAAACTCTA CCAGCAAGCC 
CAAGTTCCAT CTGTTGATTT CCAGTGCTTT TTAGAAACAA ATGAAGGGTT GAAGAAGTTT 
CTGCAGAACT TTTTGCTGTA TGGAATTGCA TTTGTAGAAA ATGTCCCTCC CACTGAAGAG 
CATACAGAAA AATTGGCAGA AAGGATTAGC TTAATCAGAG AAACCATCTA TGGAAGGATG 
TGGTATTTCA CTTCAGATTT TTCCAGAGGT GACACTGCAT ACACCAAGCT AGCTCTAGAC 
CGGCATACTG ACACTACCTA CTTTCAAGAG CCCTGTGGCA TTCAAGTATT TCATTGTCTT 
AAGCATGAAG GAACAGGTGG TAGAACACTG TTAGTGGATG GATTCTATGC AGCACAACAA 
GTACTTCAAA AAGCACCTGA GGAATTTGAA CTCCTCAGCA AAGTACCATT GAAGCATGAA 
TATATTGAAA ATGTTGGACA ATGTCACAAT CACATGATTG GGGTTGGGCC CATCTTAAAT 
ATTTACCCAT GGAATAAAGA GCTGTATTTG ATCAGGTACA ACAACTATGA CAGGGCTGTC 
ATCAACACTG TGCCTTATGA TGTTGTCCAT CGTTGGTATA CAGCACACCG AACCCTAACA 
ACTGAATTGA GAAGGCCTGA GAATGAGCTT TGGGTCAAAT TGAAGCCTGG AAAGGTACTA 
TTTATAGACA ACTGGCGAGT ACTTCATGGC AGGGAATCAT TCACTGGCTA CCGTCAACTC 
TGTGGCTGCT ATTTAACTAG AGATGATGTA TTGAATACTG CTCGTCTCCT GGGTCTTCAT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmlhe ( NM_138758.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_620097.1 Mus musculus trimethyllysine hydroxylase, epsilon (Tmlhe), mRNA.

Price & Availability↑

CloneID	OMu14505
Clone ID Related Accession (Same CDS sequence)	NM_138758.1 , NM_138758.2
Accession Version	NM_138758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569340800000
Organism	Mus musculus(house mouse)
Product	trimethyllysine dioxygenase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK052425.1, AK161724.1 and CAAA01120074.1. On Jul 26, 2018 this sequence version replaced NM_138758.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC010495.1, AK144627.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGTACC ATAAGTTGTT GCACCAACAA AGCAGGCTCC GTAATTTGAT GAAGAGAGGA 
AACATAGCTC AAGGTCTTCA TCTGTCCAAC TTTAAAAGTT TATTTTCATC ATCCATCCAT 
TGGTGCCATA CAACTTCCAA GTCTGTGAAC TGTACTTGGC ACCAACATGA AGATCATTTG 
GAACTGCAAT ATGCTGGCAC TGTGATGCGC TTTGATTATG TCTGGCTTCG AGACCATTGC 
CGCTCAGCAT CATGCTATAA TTCTAAGACT CACCAGCGGA GCCTGGACAC TGCCAGTGTG 
GATTTATGTA TTAAGCCAAA GACAGTTCAT CTGGATGAAA CTATGCTCTT TTTCACTTGG 
CCAGATGGTC ATGTGACTAG ATATGATCTG GATTGGCTGG TGAAAAACAG TTATGAAGGG 
CAGAAACAAA AGGTTATCCA ACCTAGAATA TTATGGAATT CTAAACTCTA CCAGCAAGCC 
CAAGTTCCAT CTGTTGATTT CCAGTGCTTT TTAGAAACAA ATGAAGGGTT GAAGAAGTTT 
CTGCAGAACT TTTTGCTGTA TGGAATTGCA TTTGTAGAAA ATGTCCCTCC CACTGAAGAG 
CATACAGAAA AATTGGCAGA AAGGATTAGC TTAATCAGAG AAACCATCTA TGGAAGGATG 
TGGTATTTCA CTTCAGATTT TTCCAGAGGT GACACTGCAT ACACCAAGCT AGCTCTAGAC 
CGGCATACTG ACACTACCTA CTTTCAAGAG CCCTGTGGCA TTCAAGTATT TCATTGTCTT 
AAGCATGAAG GAACAGGTGG TAGAACACTG TTAGTGGATG GATTCTATGC AGCACAACAA 
GTACTTCAAA AAGCACCTGA GGAATTTGAA CTCCTCAGCA AAGTACCATT GAAGCATGAA 
TATATTGAAA ATGTTGGACA ATGTCACAAT CACATGATTG GGGTTGGGCC CATCTTAAAT 
ATTTACCCAT GGAATAAAGA GCTGTATTTG ATCAGGTACA ACAACTATGA CAGGGCTGTC 
ATCAACACTG TGCCTTATGA TGTTGTCCAT CGTTGGTATA CAGCACACCG AACCCTAACA 
ACTGAATTGA GAAGGCCTGA GAATGAGCTT TGGGTCAAAT TGAAGCCTGG AAAGGTACTA 
TTTATAGACA ACTGGCGAGT ACTTCATGGC AGGGAATCAT TCACTGGCTA CCGTCAACTC 
TGTGGCTGCT ATTTAACTAG AGATGATGTA TTGAATACTG CTCGTCTCCT GGGTCTTCAT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620097.1
CDS	131..1396
Translation	MWYHKLLHQQSRLRNLMKRGNIAQGLHLSNFKSLFSSSIHWCHTTSKSVNCTWHQHEDHLELQYAGTVMRFDYVWLRDHCRSASCYNSKTHQRSLDTASVDLCIKPKTVHLDETMLFFTWPDGHVTRYDLDWLVKNSYEGQKQKVIQPRILWNSKLYQQAQVPSVDFQCFLETNEGLKKFLQNFLLYGIAFVENVPPTEEHTEKLAERISLIRETIYGRMWYFTSDFSRGDTAYTKLALDRHTDTTYFQEPCGIQVFHCLKHEGTGGRTLLVDGFYAAQQVLQKAPEEFELLSKVPLKHEYIENVGQCHNHMIGVGPILNIYPWNKELYLIRYNNYDRAVINTVPYDVVHRWYTAHRTLTTELRRPENELWVKLKPGKVLFIDNWRVLHGRESFTGYRQLCGCYLTRDDVLNTARLLGLHA

Target ORF information:

RefSeq Version	NM_138758.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus trimethyllysine hydroxylase, epsilon (Tmlhe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138758.2

ORF Insert Sequence:

ATGTGGTACC ATAAGTTGTT GCACCAACAA AGCAGGCTCC GTAATTTGAT GAAGAGAGGA 
AACATAGCTC AAGGTCTTCA TCTGTCCAAC TTTAAAAGTT TATTTTCATC ATCCATCCAT 
TGGTGCCATA CAACTTCCAA GTCTGTGAAC TGTACTTGGC ACCAACATGA AGATCATTTG 
GAACTGCAAT ATGCTGGCAC TGTGATGCGC TTTGATTATG TCTGGCTTCG AGACCATTGC 
CGCTCAGCAT CATGCTATAA TTCTAAGACT CACCAGCGGA GCCTGGACAC TGCCAGTGTG 
GATTTATGTA TTAAGCCAAA GACAGTTCAT CTGGATGAAA CTATGCTCTT TTTCACTTGG 
CCAGATGGTC ATGTGACTAG ATATGATCTG GATTGGCTGG TGAAAAACAG TTATGAAGGG 
CAGAAACAAA AGGTTATCCA ACCTAGAATA TTATGGAATT CTAAACTCTA CCAGCAAGCC 
CAAGTTCCAT CTGTTGATTT CCAGTGCTTT TTAGAAACAA ATGAAGGGTT GAAGAAGTTT 
CTGCAGAACT TTTTGCTGTA TGGAATTGCA TTTGTAGAAA ATGTCCCTCC CACTGAAGAG 
CATACAGAAA AATTGGCAGA AAGGATTAGC TTAATCAGAG AAACCATCTA TGGAAGGATG 
TGGTATTTCA CTTCAGATTT TTCCAGAGGT GACACTGCAT ACACCAAGCT AGCTCTAGAC 
CGGCATACTG ACACTACCTA CTTTCAAGAG CCCTGTGGCA TTCAAGTATT TCATTGTCTT 
AAGCATGAAG GAACAGGTGG TAGAACACTG TTAGTGGATG GATTCTATGC AGCACAACAA 
GTACTTCAAA AAGCACCTGA GGAATTTGAA CTCCTCAGCA AAGTACCATT GAAGCATGAA 
TATATTGAAA ATGTTGGACA ATGTCACAAT CACATGATTG GGGTTGGGCC CATCTTAAAT 
ATTTACCCAT GGAATAAAGA GCTGTATTTG ATCAGGTACA ACAACTATGA CAGGGCTGTC 
ATCAACACTG TGCCTTATGA TGTTGTCCAT CGTTGGTATA CAGCACACCG AACCCTAACA 
ACTGAATTGA GAAGGCCTGA GAATGAGCTT TGGGTCAAAT TGAAGCCTGG AAAGGTACTA 
TTTATAGACA ACTGGCGAGT ACTTCATGGC AGGGAATCAT TCACTGGCTA CCGTCAACTC 
TGTGGCTGCT ATTTAACTAG AGATGATGTA TTGAATACTG CTCGTCTCCT GGGTCTTCAT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmlhe ( XM_006535854.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535917.1 Mus musculus trimethyllysine hydroxylase, epsilon (Tmlhe), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu57230
Clone ID Related Accession (Same CDS sequence)	XM_006535854.3 , XM_006535854.4
Accession Version	XM_006535854.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	trimethyllysine dioxygenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_165789.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006535854.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTACC ATAAGTTGTT GCACCAACAA AGCAGGCTCC GTAATTTGAT GAAGAGAGGA 
AACATAGCTC AAGGTCTTCA TCTGTCCAAC TTTAAAAGTT TATTTTCATC ATCCATCCAT 
TGGTGCCATA CAACTTCCAA GTCTGTGAAC TGTACTTGGC ACCAACATGA AGATCATTTG 
GAACTGCAAT ATGCTGGCAC TGTGATGCGC TTTGATTATG TCTGGCTTCG AGACCATTGC 
CGCTCAGCAT CATGCTATAA TTCTAAGACT CACCAGCGGA GCCTGGACAC TGCCAGTGTG 
GATTTATGTA TTAAGCCAAA GACAGTTCAT CTGGATGAAA CTATGCTCTT TTTCACTTGG 
CCAGATGGTC ATGTGACTAG ATATGATCTG GATTGGCTGG TGAAAAACAG TTATGAAGGG 
CAGAAACAAA AGGTTATCCA ACCTAGAATA TTATGGAATT CTAAACTCTA CCAGCAAGCC 
CAAGTTCCAT CTGTTGATTT CCAGTGCTTT TTAGAAACAA ATGAAGGGTT GAAGAAGTTT 
CTGCAGAACT TTTTGCTGTA TGGAATTGCA TTTGTAGAAA ATGTCCCTCC CACTGAAGAG 
CATACAGAAA AATTGGCAGA AAGGATTAGC TTAATCAGAG AAACCATCTA TGGAAGGATG 
TGGTATTTCA CTTCAGATTT TTCCAGAGGT GACACTGCAT ACACCAAGCT AGCTCTAGAC 
CGGCATACTG ACACTACCTA CTTTCAAGAG CCCTGTGGGT ACAACAACTA TGACAGGGCT 
GTCATCAACA CTGTGCCTTA TGATGTTGTC CATCGTTGGT ATACAGCACA CCGAACCCTA 
ACAACTGAAT TGAGAAGGCC TGAGAATGAG CTTTGGGTCA AATTGAAGCC TGGAAAGGTA 
CTATTTATAG ACAACTGGCG AGTACTTCAT GGCAGGGAAT CATTCACTGG CTACCGTCAA 
CTCTGTGGCT GCTATTTAAC TAGAGATGAT GTATTGAATA CTGCTCGTCT CCTGGGTCTT 
CATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535917.1
CDS	172..1200
Translation	MWYHKLLHQQSRLRNLMKRGNIAQGLHLSNFKSLFSSSIHWCHTTSKSVNCTWHQHEDHLELQYAGTVMRFDYVWLRDHCRSASCYNSKTHQRSLDTASVDLCIKPKTVHLDETMLFFTWPDGHVTRYDLDWLVKNSYEGQKQKVIQPRILWNSKLYQQAQVPSVDFQCFLETNEGLKKFLQNFLLYGIAFVENVPPTEEHTEKLAERISLIRETIYGRMWYFTSDFSRGDTAYTKLALDRHTDTTYFQEPCGYNNYDRAVINTVPYDVVHRWYTAHRTLTTELRRPENELWVKLKPGKVLFIDNWRVLHGRESFTGYRQLCGCYLTRDDVLNTARLLGLHA

Target ORF information:

RefSeq Version	XM_006535854.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus trimethyllysine hydroxylase, epsilon (Tmlhe), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535854.3

ORF Insert Sequence:

ATGTGGTACC ATAAGTTGTT GCACCAACAA AGCAGGCTCC GTAATTTGAT GAAGAGAGGA 
AACATAGCTC AAGGTCTTCA TCTGTCCAAC TTTAAAAGTT TATTTTCATC ATCCATCCAT 
TGGTGCCATA CAACTTCCAA GTCTGTGAAC TGTACTTGGC ACCAACATGA AGATCATTTG 
GAACTGCAAT ATGCTGGCAC TGTGATGCGC TTTGATTATG TCTGGCTTCG AGACCATTGC 
CGCTCAGCAT CATGCTATAA TTCTAAGACT CACCAGCGGA GCCTGGACAC TGCCAGTGTG 
GATTTATGTA TTAAGCCAAA GACAGTTCAT CTGGATGAAA CTATGCTCTT TTTCACTTGG 
CCAGATGGTC ATGTGACTAG ATATGATCTG GATTGGCTGG TGAAAAACAG TTATGAAGGG 
CAGAAACAAA AGGTTATCCA ACCTAGAATA TTATGGAATT CTAAACTCTA CCAGCAAGCC 
CAAGTTCCAT CTGTTGATTT CCAGTGCTTT TTAGAAACAA ATGAAGGGTT GAAGAAGTTT 
CTGCAGAACT TTTTGCTGTA TGGAATTGCA TTTGTAGAAA ATGTCCCTCC CACTGAAGAG 
CATACAGAAA AATTGGCAGA AAGGATTAGC TTAATCAGAG AAACCATCTA TGGAAGGATG 
TGGTATTTCA CTTCAGATTT TTCCAGAGGT GACACTGCAT ACACCAAGCT AGCTCTAGAC 
CGGCATACTG ACACTACCTA CTTTCAAGAG CCCTGTGGGT ACAACAACTA TGACAGGGCT 
GTCATCAACA CTGTGCCTTA TGATGTTGTC CATCGTTGGT ATACAGCACA CCGAACCCTA 
ACAACTGAAT TGAGAAGGCC TGAGAATGAG CTTTGGGTCA AATTGAAGCC TGGAAAGGTA 
CTATTTATAG ACAACTGGCG AGTACTTCAT GGCAGGGAAT CATTCACTGG CTACCGTCAA 
CTCTGTGGCT GCTATTTAAC TAGAGATGAT GTATTGAATA CTGCTCGTCT CCTGGGTCTT 
CATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmlhe ( XM_006535854.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535917.1 Mus musculus trimethyllysine hydroxylase, epsilon (Tmlhe), transcript variant X1, mRNA.

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CloneID	OMu57230
Clone ID Related Accession (Same CDS sequence)	XM_006535854.3 , XM_006535854.4
Accession Version	XM_006535854.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	trimethyllysine dioxygenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_165789.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006535854.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTACC ATAAGTTGTT GCACCAACAA AGCAGGCTCC GTAATTTGAT GAAGAGAGGA 
AACATAGCTC AAGGTCTTCA TCTGTCCAAC TTTAAAAGTT TATTTTCATC ATCCATCCAT 
TGGTGCCATA CAACTTCCAA GTCTGTGAAC TGTACTTGGC ACCAACATGA AGATCATTTG 
GAACTGCAAT ATGCTGGCAC TGTGATGCGC TTTGATTATG TCTGGCTTCG AGACCATTGC 
CGCTCAGCAT CATGCTATAA TTCTAAGACT CACCAGCGGA GCCTGGACAC TGCCAGTGTG 
GATTTATGTA TTAAGCCAAA GACAGTTCAT CTGGATGAAA CTATGCTCTT TTTCACTTGG 
CCAGATGGTC ATGTGACTAG ATATGATCTG GATTGGCTGG TGAAAAACAG TTATGAAGGG 
CAGAAACAAA AGGTTATCCA ACCTAGAATA TTATGGAATT CTAAACTCTA CCAGCAAGCC 
CAAGTTCCAT CTGTTGATTT CCAGTGCTTT TTAGAAACAA ATGAAGGGTT GAAGAAGTTT 
CTGCAGAACT TTTTGCTGTA TGGAATTGCA TTTGTAGAAA ATGTCCCTCC CACTGAAGAG 
CATACAGAAA AATTGGCAGA AAGGATTAGC TTAATCAGAG AAACCATCTA TGGAAGGATG 
TGGTATTTCA CTTCAGATTT TTCCAGAGGT GACACTGCAT ACACCAAGCT AGCTCTAGAC 
CGGCATACTG ACACTACCTA CTTTCAAGAG CCCTGTGGGT ACAACAACTA TGACAGGGCT 
GTCATCAACA CTGTGCCTTA TGATGTTGTC CATCGTTGGT ATACAGCACA CCGAACCCTA 
ACAACTGAAT TGAGAAGGCC TGAGAATGAG CTTTGGGTCA AATTGAAGCC TGGAAAGGTA 
CTATTTATAG ACAACTGGCG AGTACTTCAT GGCAGGGAAT CATTCACTGG CTACCGTCAA 
CTCTGTGGCT GCTATTTAAC TAGAGATGAT GTATTGAATA CTGCTCGTCT CCTGGGTCTT 
CATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535917.1
CDS	171..1199
Translation	MWYHKLLHQQSRLRNLMKRGNIAQGLHLSNFKSLFSSSIHWCHTTSKSVNCTWHQHEDHLELQYAGTVMRFDYVWLRDHCRSASCYNSKTHQRSLDTASVDLCIKPKTVHLDETMLFFTWPDGHVTRYDLDWLVKNSYEGQKQKVIQPRILWNSKLYQQAQVPSVDFQCFLETNEGLKKFLQNFLLYGIAFVENVPPTEEHTEKLAERISLIRETIYGRMWYFTSDFSRGDTAYTKLALDRHTDTTYFQEPCGYNNYDRAVINTVPYDVVHRWYTAHRTLTTELRRPENELWVKLKPGKVLFIDNWRVLHGRESFTGYRQLCGCYLTRDDVLNTARLLGLHA

Target ORF information:

RefSeq Version	XM_006535854.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus trimethyllysine hydroxylase, epsilon (Tmlhe), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535854.4

ORF Insert Sequence:

ATGTGGTACC ATAAGTTGTT GCACCAACAA AGCAGGCTCC GTAATTTGAT GAAGAGAGGA 
AACATAGCTC AAGGTCTTCA TCTGTCCAAC TTTAAAAGTT TATTTTCATC ATCCATCCAT 
TGGTGCCATA CAACTTCCAA GTCTGTGAAC TGTACTTGGC ACCAACATGA AGATCATTTG 
GAACTGCAAT ATGCTGGCAC TGTGATGCGC TTTGATTATG TCTGGCTTCG AGACCATTGC 
CGCTCAGCAT CATGCTATAA TTCTAAGACT CACCAGCGGA GCCTGGACAC TGCCAGTGTG 
GATTTATGTA TTAAGCCAAA GACAGTTCAT CTGGATGAAA CTATGCTCTT TTTCACTTGG 
CCAGATGGTC ATGTGACTAG ATATGATCTG GATTGGCTGG TGAAAAACAG TTATGAAGGG 
CAGAAACAAA AGGTTATCCA ACCTAGAATA TTATGGAATT CTAAACTCTA CCAGCAAGCC 
CAAGTTCCAT CTGTTGATTT CCAGTGCTTT TTAGAAACAA ATGAAGGGTT GAAGAAGTTT 
CTGCAGAACT TTTTGCTGTA TGGAATTGCA TTTGTAGAAA ATGTCCCTCC CACTGAAGAG 
CATACAGAAA AATTGGCAGA AAGGATTAGC TTAATCAGAG AAACCATCTA TGGAAGGATG 
TGGTATTTCA CTTCAGATTT TTCCAGAGGT GACACTGCAT ACACCAAGCT AGCTCTAGAC 
CGGCATACTG ACACTACCTA CTTTCAAGAG CCCTGTGGGT ACAACAACTA TGACAGGGCT 
GTCATCAACA CTGTGCCTTA TGATGTTGTC CATCGTTGGT ATACAGCACA CCGAACCCTA 
ACAACTGAAT TGAGAAGGCC TGAGAATGAG CTTTGGGTCA AATTGAAGCC TGGAAAGGTA 
CTATTTATAG ACAACTGGCG AGTACTTCAT GGCAGGGAAT CATTCACTGG CTACCGTCAA 
CTCTGTGGCT GCTATTTAAC TAGAGATGAT GTATTGAATA CTGCTCGTCT CCTGGGTCTT 
CATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional characterization of the TMLH gene: promoter analysis, in situ hybridization, identification and mapping of alternative splicing variants.
Gene395(1-2)86-97(2007 Jun)
Monfregola J,Napolitano G,Conte I,Cevenini A,Migliaccio C,D'Urso M,Ursini MV

Functional analysis of TMLH variants and definition of domains required for catalytic activity and mitochondrial targeting.
Journal of cellular physiology204(3)839-47(2005 Sep)
Monfregola J,Cevenini A,Terracciano A,van Vlies N,Arbucci S,Wanders RJ,D'Urso M,Vaz FM,Ursini MV

Structural basis of allotypes of ecto-nucleotide pyrophosphatase/phosphodiesterase (plasma cell membrane glycoprotein PC-1) in the mouse and rat, and analysis of allele-specific xenogeneic antibodies.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics29(4)307-13(2002 Aug)
Banakh I,Sali A,Dubljevic V,Grobben B,Slegers H,Goding JW

Molecular and Biochemical Characterization of Rat epsilon -N-Trimethyllysine Hydroxylase, the First Enzyme of Carnitine Biosynthesis.
The Journal of biological chemistry276(36)33512-7(2001 Sep)
Vaz FM,Ofman R,Westinga K,Back JW,Wanders RJ

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Tmlhe cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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