Flna cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Flna
Entrez Gene ID	192176
Full Name	filamin, alpha
Synonyms	ABP-280,Dilp2,F730004A14Rik,Fln1,GENA 379,filamin-1
General protein information	Preferred Names filamin, alpha Names filamin-A actin-binding protein 280 dilated pupils 2 endothelial actin-binding protein non-muscle filamin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X A7.3\|X 37.89 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017318433.1	XP_017173922.1	filamin-A isoform X3
XM_017318433.2	XP_017173922.1	filamin-A isoform X3
XM_011247549.2	XP_011245851.1	filamin-A isoform X2
XM_011247549.3	XP_011245851.1	filamin-A isoform X2
XM_006527911.4	XP_006527974.1	filamin-A isoform X1
NM_010227.3	NP_034357.2	filamin-A isoform 1
NM_001290421.1	NP_001277350.1	filamin-A isoform 2
XM_006527911.3	XP_006527974.1	filamin-A isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	FLNA	NP_001193443.1
Mus musculus (house mouse)	Flna	NP_034357.2
Drosophila melanogaster (fruit fly)	cher	NP_001262658.1
Caenorhabditis elegans (roundworm)	fln-1	NP_001023550.2
Homo sapiens (human)	FLNA	NP_001104026.1
Macaca mulatta (Rhesus monkey)	FLNA	XP_001091073.1
Canis lupus familiaris (dog)	FLNA	XP_867483.1
Rattus norvegicus (Norway rat)	Flna	NP_001128071.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004335	XP_313613.5
Gallus gallus (chicken)	FLNA	NP_989905.1

Gene Ontology
Bibliography

Related articles in PubMed

Hypoxia-induced interaction of filamin with Drp1 causes mitochondrial hyperfission-associated myocardial senescence.
Nishimura A, Shimauchi T, Tanaka T, Shimoda K, Toyama T, Kitajima N, Ishikawa T, Shindo N, Numaga-Tomita T, Yasuda S, Sato Y, Kuwahara K, Kumagai Y, Akaike T, Ide T, Ojida A, Mori Y, Nishida M
Science signaling11(556)(2018 11)

RNA editing of Filamin A pre-mRNA regulates vascular contraction and diastolic blood pressure.
Jain M, Mann TD, Stulić M, Rao SP, Kirsch A, Pullirsch D, Strobl X, Rath C, Reissig L, Moreth K, Klein-Rodewald T, Bekeredjian R, Gailus-Durner V, Fuchs H, Hrabě de Angelis M, Pablik E, Cimatti L, Martin D, Zinnanti J, Graier WF, Sibilia M, Frank S, Levanon EY, Jantsch MF
The EMBO journal37(19)(2018 10)

IRE1α governs cytoskeleton remodelling and cell migration through a direct interaction with filamin A.
Urra H, Henriquez DR, Cánovas J, Villarroel-Campos D, Carreras-Sureda A, Pulgar E, Molina E, Hazari YM, Limia CM, Alvarez-Rojas S, Figueroa R, Vidal RL, Rodriguez DA, Rivera CA, Court FA, Couve A, Qi L, Chevet E, Akai R, Iwawaki T, Concha ML, Glavic Á, Gonzalez-Billault C, Hetz C
Nature cell biology20(8)942-953(2018 08)

Filamin A Is Required for Optimal T Cell Integrin-Mediated Force Transmission, Flow Adhesion, and T Cell Trafficking.
Savinko T, Guenther C, Uotila LM, Llort Asens M, Yao S, Tojkander S, Fagerholm SC
Journal of immunology (Baltimore, Md. : 1950)200(9)3109-3116(2018 05)

Filamin A Phosphorylation at Serine 2152 by the Serine/Threonine Kinase Ndr2 Controls TCR-Induced LFA-1 Activation in T Cells.
Waldt N, Seifert A, Demiray YE, Devroe E, Turk BE, Reichardt P, Mix C, Reinhold A, Freund C, Müller AJ, Schraven B, Stork O, Kliche S
Frontiers in immunology92852(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Flna gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Flna cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20537	NM_001290421.1 Latest version!	Mus musculus filamin, alpha (Flna), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu20537	XM_017318433.2 Latest version!	Mus musculus filamin, alpha (Flna), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu20363	NM_010227.3 Latest version!	Mus musculus filamin, alpha (Flna), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu20363	XM_011247549.3 Latest version!	Mus musculus filamin, alpha (Flna), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu56214	XM_006527911.4 Latest version!	Mus musculus filamin, alpha (Flna), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OMu20537	XM_017318433.1	Mus musculus filamin, alpha (Flna), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu20363	XM_011247549.2	Mus musculus filamin, alpha (Flna), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu56214	XM_006527911.3	Mus musculus filamin, alpha (Flna), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Flna ( NM_001290421.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277350.1 Mus musculus filamin, alpha (Flna), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20537
Clone ID Related Accession (Same CDS sequence)	XM_017318433.1 , XM_017318433.2 , NM_001290421.1
Accession Version	NM_001290421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Mus musculus(house mouse)
Product	filamin-A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL807376.4. Transcript Variant: This variant (2) contains multiple differences compared to variant 1, including initiation of translation at a downstream in-frame start codon. The encoded isoform (2) has a shorter N-terminus than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164133 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACA TCCGTGAGGC ACCCCAGGAT TTTCACCCAG ACAGGGTGAA GGCACGTGGG 
CCTGGATTGG AGAAGACTGG TGTGGCTGTC AACAAGCCAG CAGAGTTCAC AGTTGATGCC 
AAGCATGCTG GGAAGGCTCC TCTTCGAGTT CAAGTTCAGG ACAATGAGGG CTGCTCTGTG 
GAAGCGACAG TCAAGGACAA TGGCAATGGT ACTTACAGCT GTTCTTATGT GCCCAGAAAG 
CCAGTGAAGC ACACAGCCAT GGTTTCTTGG GGAGGTGTCA GCATCCCCAA CAGTCCTTTC 
CGGGTGAATG TGGGAGCTGG CAGCCATCCA AACAAAGTCA AGGTGTATGG TCCAGGAGTG 
GCCAAGACTG GGCTCAAGGC CCATGAACCT ACCTACTTTA CTGTGGATTG TACTGAAGCT 
GGCCAGGGAG ATGTCAGCAT TGGTATCAAG TGTGCCCCTG GAGTAGTGGG CCCCACTGAG 
GCTGATATTG ACTTTGATAT CATCCGCAAT GACAATGACA CCTTCACTGT AAAATACACA 
CCCTGTGGGG CTGGCAGCTA TACCATCATG GTCCTTTTTG CTGACCAGGC CACACCCACC 
AGCCCCATCA GAGTCAAAGT GGAGCCTTCT CATGATGCCA GCAAGGTGAA GGCTGAGGGT 
CCTGGCCTAA ATCGCACTGG TGTTGAACTT GGCAAACCCA CCCATTTCAC AGTCAATGCT 
AAAACTGCTG GGAAAGGCAA GCTGGATGTC CAATTCTCAG GACTGGCTAA GGGAGATGCA 
GTACGGGATG TGGACATCAT TGACCACCAT GATAATACCT ACACAGTCAA GTACATTCCT 
GTGCAGCAGG GCCCAGTAGG TGTCAATGTC ACTTATGGAG GAGATCACAT CCCCAAGAGT 
CCATTTTCAG TGGGAGTATC TCCAAGCCTG GATCTCAGCA AAATCAAGGT GTCTGGCCTT 
GGTGACAAAG TGGACGTTGG CAAAGATCAA GAGTTCACAG TAAAGTCAAA GGGTGCAGGT 
GGTCAAGGCA AAGTAGCATC CAAGATTGTG AGTCCCTCAG GTGCAGCGGT ACCCTGCAAG 
GTAGAGCCAG GCCTGGGAGC TGACAACAGC GTGGTACGTT TTGTGCCCCG TGAAGAGGGG 
CCCTATGAGG TGGAAGTGAC CTATGATGGT GTGCCTGTAC CTGGCAGTCC CTTTCCACTA 
GAAGCTGTGG CCCCCACCAA ACCCAGCAAG GTGAAGGCGT TTGGACCAGG GCTACAGGGG 
GGCAATGCAG GCTCCCCTGC CCGCTTCACC ATTGATACAA AGGGTGCTGG CACTGGTGGC 
CTGGGCCTGA CAGTGGAAGG CCCCTGTGAA GCACAGCTTG AGTGCCTAGA CAACGGGGAT 
GGTACATGCT CTGTGTCTTA TGTACCCACT GAGCCTGGGG ACTACAACAT CAACATCCTT 
TTTGCTGACA CCCACATTCC TGGATCCCCA TTCAAGGCCC ATGTGGCTCC TTGTTTTGAT 
GCATCCAAGG TGAAGTGCTC AGGCCCTGGG CTGGAGCGGG CTACTGCTGG TGAGGTAGGG 
CAGTTCCAAG TGGACTGTTC AAGTGCTGGC AGTGCTGAGT TGACGATTGA GATCTGCTCT 
GAGGCAGGAC TGCCAGCTGA AGTATACATT CAAGACCATG GTGATGGCAC ACACACCATT 
ACCTATATTC CTCTCTGTCC TGGGGCTTAC ACTGTTACCA TCAAGTATGG CGGCCAGCCT 
GTGCCCAACT TCCCCAGCAA GCTACAGGTG GAACCTGCTG TAGATACCTC AGGTGTACAG 
TGCTATGGGC CTGGGATTGA AGGTCAAGGT GTCTTCCGAG AGGCAACCAC TGAGTTCAGT 
GTGGATGCCC GGGCTCTTAC ACAGACTGGA GGGCCACATG TCAAGGCTCG AGTGGCCAAC 
CCCTCAGGCA ATCTGACAGA TACCTATGTG CAAGACTGTG GGGATGGCAC ATACAAAGTG 
GAATACACTC CATATGAGGA AGGAGTACAC TCTGTGGATG TGACTTATGA TGGCAGCCCT 
GTGCCCAGCA GCCCCTTCCA GGTGCCTGTA ACAGAGGGCT GTGACCCCTC CCGGGTGCGT 
GTCCATGGAC CAGGCATCCA AAGTGGTACC ACCAACAAAC CCAACAAGTT CACAGTAGAA 
ACCAGGGGAG CTGGCACAGG TGGCCTGGGC TTGGCTGTTG AGGGTCCCTC AGAGGCCAAG 
ATGTCTTGTA TGGATAATAA AGATGGCAGC TGCTCAGTAG AATACATCCC CTATGAAGCT 
GGAACCTATA GCCTTAATGT CACTTATGGT GGTCACCAAG TGCCAGGTAG TCCCTTCAAG 
GTCCCTGTAC ATGATGTGAC AGATGCATCT AAAGTCAAGT GTTCTGGACC TGGCCTAAGC 
CCAGGCATGG TCCGTGCCAA CCTCCCTCAG TCCTTTCAGG TGGACACAAG CAAAGCTGGA 
GTTGCCCCAC TGCAGGTCAA AGTGCAGGGG CCCAAAGGCC TGGTGGAGCC AGTGGATGTA 
GTGGACAATG CTGATGGTAC TCAGACTGTC AACTATGTGC CCAGCCGAGA AGGGTCCTAT 
AGCATTTCTG TGCTGTATGG TGAAGAAGAA GTGCCACGGA GCCCCTTCAA GGTCAAGGTG 
CTGCCTACAC ATGATGCCAG TAAGGTGAAG GCCAGTGGAC CTGGACTCAA CACCACTGGT 
GTACCTGCTA GCCTGCCTGT GGAGTTCACC ATTGATGCCA AGGATGCTGG GGAGGGTCTG 
TTGGCTGTCC AGATTACGGA TCCTGAAGGC AAGCCCAAGA AGACACACAT TCAAGATAAT 
CATGATGGCA CATACACGGT GGCTTATGTG CCAGATGTGC CAGGCCGGTA CACAATCCTC 
ATCAAGTATG GTGGTGATGA GATTCCCTTT TCCCCGTACC GTGTCCGGGC TGTGCCCACT 
GGGGATGCCA GCAAGTGCAC AGTCACAGTG TCAATCGGAG GTCACGGGCT AGGTGCTGGC 
ATTGGCCCCA CCATCCAGAT TGGGGAGGAG ACGGTGATTA CTGTGGACAC AAAAGCAGCA 
GGCAAAGGCA AAGTGACTTG TACTGTGTGC ACACCTGATG GCTCAGAGGT AGACGTGGAC 
GTGGTGGAGA ATGAGGATGG CACCTTTGAC ATCTTCTACA CAGCTCCCCA ACCGGGCAAA 
TATGTCATCT GTGTGCGCTT CGGTGGCGAG CATGTGCCCA ACAGCCCCTT CCAAGTTACA 
GCTTTGGCTG GGGACCAACC AACAGTGCAG ACCCCATTAA GGTCTCAGCA GCTGGCTCCA 
CAGTATAACT ATCCTCAGGG TAGCCAGCAA ACCTGGATTC CAGAGAGGCC CATGGTGGGC 
GTTAATGGGC TGGATGTGAC CAGCCTGAGG CCCTTTGATC TTGTCATCCC CTTCACTATC 
AAGAAGGGGG AGATCACTGG GGAAGTTCGA ATGCCCTCAG GCAAGGTGGC CCAGCCTTCC 
ATTACTGATA ACAAAGATGG CACTGTTACT GTACGTTACT CACCCAGTGA AGCTGGCCTG 
CATGAAATGG ACATTCGCTA TGACAATATG CATATCCCAG GAAGCCCTCT GCAGTTCTAT 
GTTGATTATG TCAACTGTGG CCACATCACT GCTTATGGTC CTGGCCTTAC CCATGGAGTG 
GTCAACAAAC CTGCCACCTT CACTGTCAAC ACCAAGGATG CAGGAGAGGG GGGCTTGTCT 
CTGGCCATTG AGGGTCCATC TAAAGCAGAA ATCAGTTGCA CTGACAACCA GGATGGAACA 
TGCAGTGTCT CTTACCTGCC TGTACTGCCT GGTGACTATA GCATCCTAGT TAAGTACAAT 
GATCAACACA TCCCAGGCAG TCCCTTTACT GCCAGAGTAA CAGGTGACGA TTCCATGCGT 
ATGTCCCACC TAAAGGTGGG TTCTGCTGCT GATATCCCCA TCAATATCTC AGAAACAGAC 
CTTAGCCTAC TCACAGCCAC TGTGGTGCCA CCTTCGGGTC GAGAGGAACC CTGTCTGCTG 
AAACGTTTGC GAAATGGCCA CGTGGGGATT TCCTTCGTGC CCAAGGAGAC AGGGGAGCAC 
CTGGTACATG TGAAGAAGAA TGGCCAGCAT GTGGCAAGCA GTCCCATCCC AGTAGTGATC 
AGCCAGTCGG AGATAGGTGA TGCCAGCCGT GTGAGGGTCT CTGGTCAAGG TCTTCATGAA 
GGTCATACCT TTGAGCCTGC AGAGTTTATT ATTGACACCA GAGATGCAGG CTACGGTGGG 
CTTAGTCTGT CCATTGAGGG CCCTAGCAAA GTAGACATCA ACACAGAGGA TCTGGAGGAT 
GGCACATGCA GGGTCACCTA CTGTCCCACA GAGCCTGGAA ACTACATTAT AAACATCAAA 
TTTGCTGACC AGCATGTGCC TGGCAGTCCC TTTTCTGTGA AGGTGACAGG TGAGGGCCGG 
GTGAAAGAGA GTATCACACG CAGGCGACGT GCCCCTTCTG TGGCCAATAT TGGCAGTCAT 
TGTGACCTCA GCCTGAAGAT TCCTGAAATT AGCATCCAAG ATATGACAGC CCAGGTGACC 
AGCCCATCAG GCAAGACCCA TGAGGCAGAG ATCGTAGAAG GAGAGAACCA TACTTACTGT 
ATCCGATTTG TGCCTGCTGA GATGGGAATG CATACAGTCA GTGTCAAGTA CAAGGGCCAG 
CATGTACCTG GGAGCCCCTT CCAGTTCACT GTGGGGCCTC TGGGGGAAGG GGGTGCTCAC 
AAGGTCCGTG CTGGAGGCCC TGGCCTAGAG AGGGCTGAAG TTGGAGTGCC AGCGGAGTTC 
GGCATTTGGA CTAGGGAAGC TGGCGCTGGA GGCCTGGCCA TTGCTGTTGA AGGCCCCAGC 
AAGGCTGAGA TCTCTTTCGA GGACCGAAAG GATGGCTCCT GTGGTGTGGC CTACGTAGTT 
CAGGAGCCAG GTGACTATGA GGTCTCAGTC AAGTTCAACG AGGAGCACAT ACCTGATAGC 
CCCTTCGTGG TGCCTGTGGC TTCTCCGTCT GGTGACGCCC GCCGCCTTAC TGTTTCTAGT 
CTTCAGGAGT CAGGGTTAAA GGTCAACCAG CCAGCATCTT TTGCAGTCAG TCTGAATGGA 
GCCAAGGGGG CAATTGATGC CAAGGTGCAC AGCCCCTCAG GAGCTCTGGA GGAGTGCTAT 
GTCACAGAGA TTGACCAAGA TAAGTATGCT GTGCGTTTCA TCCCACGAGA GAATGGCATC 
TACTTGATTG ATGTCAAGTT CAATGGTACT CACATTCCTG GAAGTCCCTT CAAGATCCGA 
GTTGGGGAGC CTGGGCATGG AGGGGACCCA GGCTTAGTGT CCGCCTATGG AGCAGGCCTG 
GAAGGTGGTG TCACAGGGAG CCCAGCAGAG TTTATTGTGA ACACAAGCAA TGCAGGAGCT 
GGTGCCCTTT CGGTTACCAT TGATGGCCCC TCCAAGGTGA AGATGGATTG CCAGGAGTGC 
CCCGAGGGCT ATCGTGTCAC CTATACCCCC ATGGCACCTG GCAGCTACCT CATCTCCATC 
AAGTATGGTG GCCCCTATCA CATTGGTGGA AGTCCCTTTA AAGCCAAGGT CACAGGTCCT 
CGTCTTGTAA GCAACCACAG CCTCCATGAG ACATCATCTG TGTTTGTGGA CTCTTTGACT 
AAAGTTGCCA CGGTTCCCCA GCATGCAACC TCAGGCCCAG GTCCTGCTGA TGTCAGCAAG 
GTAGTAGCCA AAGGCCTGGG GCTAAGCAAG GCTTATGTAG GCCAGAAGAG CAACTTCACA 
GTAGATTGCA GCAAAGCAGG TAACAACATG CTGCTGGTGG GCGTGCATGG CCCAAGGACA 
CCCTGTGAAG AGATCCTGGT GAAACACATG GGCAGCCGCC TCTATAGTGT CTCTTACCTG 
CTCAAGGACA AAGGGGAGTA CACACTGGTG GTCAAGTGGG GTGATGAGCA TATCCCAGGC 
AGCCCATACC GCATTATGGT ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277350.1
CDS	2074..8040
Translation	MADIREAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAKHAGKAPLRVQVQDNEGCSVEATVKDNGNGTYSCSYVPRKPVKHTAMVSWGGVSIPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCTEAGQGDVSIGIKCAPGVVGPTEADIDFDIIRNDNDTFTVKYTPCGAGSYTIMVLFADQATPTSPIRVKVEPSHDASKVKAEGPGLNRTGVELGKPTHFTVNAKTAGKGKLDVQFSGLAKGDAVRDVDIIDHHDNTYTVKYIPVQQGPVGVNVTYGGDHIPKSPFSVGVSPSLDLSKIKVSGLGDKVDVGKDQEFTVKSKGAGGQGKVASKIVSPSGAAVPCKVEPGLGADNSVVRFVPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQGGNAGSPARFTIDTKGAGTGGLGLTVEGPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVAPCFDASKVKCSGPGLERATAGEVGQFQVDCSSAGSAELTIEICSEAGLPAEVYIQDHGDGTHTITYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTGGPHVKARVANPSGNLTDTYVQDCGDGTYKVEYTPYEEGVHSVDVTYDGSPVPSSPFQVPVTEGCDPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKMSCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDVVDNADGTQTVNYVPSREGSYSISVLYGEEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVPDVPGRYTILIKYGGDEIPFSPYRVRAVPTGDASKCTVTVSIGGHGLGAGIGPTIQIGEETVITVDTKAAGKGKVTCTVCTPDGSEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPTVQTPLRSQQLAPQYNYPQGSQQTWIPERPMVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPSITDNKDGTVTVRYSPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHITAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNDQHIPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANIGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGMHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEVGVPAEFGIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGIYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGSPAEFIVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKVATVPQHATSGPGPADVSKVVAKGLGLSKAYVGQKSNFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHMGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRIMVP

Target ORF information:

RefSeq Version	NM_001290421.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus filamin, alpha (Flna), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290421.1

ORF Insert Sequence:

ATGGCTGACA TCCGTGAGGC ACCCCAGGAT TTTCACCCAG ACAGGGTGAA GGCACGTGGG 
CCTGGATTGG AGAAGACTGG TGTGGCTGTC AACAAGCCAG CAGAGTTCAC AGTTGATGCC 
AAGCATGCTG GGAAGGCTCC TCTTCGAGTT CAAGTTCAGG ACAATGAGGG CTGCTCTGTG 
GAAGCGACAG TCAAGGACAA TGGCAATGGT ACTTACAGCT GTTCTTATGT GCCCAGAAAG 
CCAGTGAAGC ACACAGCCAT GGTTTCTTGG GGAGGTGTCA GCATCCCCAA CAGTCCTTTC 
CGGGTGAATG TGGGAGCTGG CAGCCATCCA AACAAAGTCA AGGTGTATGG TCCAGGAGTG 
GCCAAGACTG GGCTCAAGGC CCATGAACCT ACCTACTTTA CTGTGGATTG TACTGAAGCT 
GGCCAGGGAG ATGTCAGCAT TGGTATCAAG TGTGCCCCTG GAGTAGTGGG CCCCACTGAG 
GCTGATATTG ACTTTGATAT CATCCGCAAT GACAATGACA CCTTCACTGT AAAATACACA 
CCCTGTGGGG CTGGCAGCTA TACCATCATG GTCCTTTTTG CTGACCAGGC CACACCCACC 
AGCCCCATCA GAGTCAAAGT GGAGCCTTCT CATGATGCCA GCAAGGTGAA GGCTGAGGGT 
CCTGGCCTAA ATCGCACTGG TGTTGAACTT GGCAAACCCA CCCATTTCAC AGTCAATGCT 
AAAACTGCTG GGAAAGGCAA GCTGGATGTC CAATTCTCAG GACTGGCTAA GGGAGATGCA 
GTACGGGATG TGGACATCAT TGACCACCAT GATAATACCT ACACAGTCAA GTACATTCCT 
GTGCAGCAGG GCCCAGTAGG TGTCAATGTC ACTTATGGAG GAGATCACAT CCCCAAGAGT 
CCATTTTCAG TGGGAGTATC TCCAAGCCTG GATCTCAGCA AAATCAAGGT GTCTGGCCTT 
GGTGACAAAG TGGACGTTGG CAAAGATCAA GAGTTCACAG TAAAGTCAAA GGGTGCAGGT 
GGTCAAGGCA AAGTAGCATC CAAGATTGTG AGTCCCTCAG GTGCAGCGGT ACCCTGCAAG 
GTAGAGCCAG GCCTGGGAGC TGACAACAGC GTGGTACGTT TTGTGCCCCG TGAAGAGGGG 
CCCTATGAGG TGGAAGTGAC CTATGATGGT GTGCCTGTAC CTGGCAGTCC CTTTCCACTA 
GAAGCTGTGG CCCCCACCAA ACCCAGCAAG GTGAAGGCGT TTGGACCAGG GCTACAGGGG 
GGCAATGCAG GCTCCCCTGC CCGCTTCACC ATTGATACAA AGGGTGCTGG CACTGGTGGC 
CTGGGCCTGA CAGTGGAAGG CCCCTGTGAA GCACAGCTTG AGTGCCTAGA CAACGGGGAT 
GGTACATGCT CTGTGTCTTA TGTACCCACT GAGCCTGGGG ACTACAACAT CAACATCCTT 
TTTGCTGACA CCCACATTCC TGGATCCCCA TTCAAGGCCC ATGTGGCTCC TTGTTTTGAT 
GCATCCAAGG TGAAGTGCTC AGGCCCTGGG CTGGAGCGGG CTACTGCTGG TGAGGTAGGG 
CAGTTCCAAG TGGACTGTTC AAGTGCTGGC AGTGCTGAGT TGACGATTGA GATCTGCTCT 
GAGGCAGGAC TGCCAGCTGA AGTATACATT CAAGACCATG GTGATGGCAC ACACACCATT 
ACCTATATTC CTCTCTGTCC TGGGGCTTAC ACTGTTACCA TCAAGTATGG CGGCCAGCCT 
GTGCCCAACT TCCCCAGCAA GCTACAGGTG GAACCTGCTG TAGATACCTC AGGTGTACAG 
TGCTATGGGC CTGGGATTGA AGGTCAAGGT GTCTTCCGAG AGGCAACCAC TGAGTTCAGT 
GTGGATGCCC GGGCTCTTAC ACAGACTGGA GGGCCACATG TCAAGGCTCG AGTGGCCAAC 
CCCTCAGGCA ATCTGACAGA TACCTATGTG CAAGACTGTG GGGATGGCAC ATACAAAGTG 
GAATACACTC CATATGAGGA AGGAGTACAC TCTGTGGATG TGACTTATGA TGGCAGCCCT 
GTGCCCAGCA GCCCCTTCCA GGTGCCTGTA ACAGAGGGCT GTGACCCCTC CCGGGTGCGT 
GTCCATGGAC CAGGCATCCA AAGTGGTACC ACCAACAAAC CCAACAAGTT CACAGTAGAA 
ACCAGGGGAG CTGGCACAGG TGGCCTGGGC TTGGCTGTTG AGGGTCCCTC AGAGGCCAAG 
ATGTCTTGTA TGGATAATAA AGATGGCAGC TGCTCAGTAG AATACATCCC CTATGAAGCT 
GGAACCTATA GCCTTAATGT CACTTATGGT GGTCACCAAG TGCCAGGTAG TCCCTTCAAG 
GTCCCTGTAC ATGATGTGAC AGATGCATCT AAAGTCAAGT GTTCTGGACC TGGCCTAAGC 
CCAGGCATGG TCCGTGCCAA CCTCCCTCAG TCCTTTCAGG TGGACACAAG CAAAGCTGGA 
GTTGCCCCAC TGCAGGTCAA AGTGCAGGGG CCCAAAGGCC TGGTGGAGCC AGTGGATGTA 
GTGGACAATG CTGATGGTAC TCAGACTGTC AACTATGTGC CCAGCCGAGA AGGGTCCTAT 
AGCATTTCTG TGCTGTATGG TGAAGAAGAA GTGCCACGGA GCCCCTTCAA GGTCAAGGTG 
CTGCCTACAC ATGATGCCAG TAAGGTGAAG GCCAGTGGAC CTGGACTCAA CACCACTGGT 
GTACCTGCTA GCCTGCCTGT GGAGTTCACC ATTGATGCCA AGGATGCTGG GGAGGGTCTG 
TTGGCTGTCC AGATTACGGA TCCTGAAGGC AAGCCCAAGA AGACACACAT TCAAGATAAT 
CATGATGGCA CATACACGGT GGCTTATGTG CCAGATGTGC CAGGCCGGTA CACAATCCTC 
ATCAAGTATG GTGGTGATGA GATTCCCTTT TCCCCGTACC GTGTCCGGGC TGTGCCCACT 
GGGGATGCCA GCAAGTGCAC AGTCACAGTG TCAATCGGAG GTCACGGGCT AGGTGCTGGC 
ATTGGCCCCA CCATCCAGAT TGGGGAGGAG ACGGTGATTA CTGTGGACAC AAAAGCAGCA 
GGCAAAGGCA AAGTGACTTG TACTGTGTGC ACACCTGATG GCTCAGAGGT AGACGTGGAC 
GTGGTGGAGA ATGAGGATGG CACCTTTGAC ATCTTCTACA CAGCTCCCCA ACCGGGCAAA 
TATGTCATCT GTGTGCGCTT CGGTGGCGAG CATGTGCCCA ACAGCCCCTT CCAAGTTACA 
GCTTTGGCTG GGGACCAACC AACAGTGCAG ACCCCATTAA GGTCTCAGCA GCTGGCTCCA 
CAGTATAACT ATCCTCAGGG TAGCCAGCAA ACCTGGATTC CAGAGAGGCC CATGGTGGGC 
GTTAATGGGC TGGATGTGAC CAGCCTGAGG CCCTTTGATC TTGTCATCCC CTTCACTATC 
AAGAAGGGGG AGATCACTGG GGAAGTTCGA ATGCCCTCAG GCAAGGTGGC CCAGCCTTCC 
ATTACTGATA ACAAAGATGG CACTGTTACT GTACGTTACT CACCCAGTGA AGCTGGCCTG 
CATGAAATGG ACATTCGCTA TGACAATATG CATATCCCAG GAAGCCCTCT GCAGTTCTAT 
GTTGATTATG TCAACTGTGG CCACATCACT GCTTATGGTC CTGGCCTTAC CCATGGAGTG 
GTCAACAAAC CTGCCACCTT CACTGTCAAC ACCAAGGATG CAGGAGAGGG GGGCTTGTCT 
CTGGCCATTG AGGGTCCATC TAAAGCAGAA ATCAGTTGCA CTGACAACCA GGATGGAACA 
TGCAGTGTCT CTTACCTGCC TGTACTGCCT GGTGACTATA GCATCCTAGT TAAGTACAAT 
GATCAACACA TCCCAGGCAG TCCCTTTACT GCCAGAGTAA CAGGTGACGA TTCCATGCGT 
ATGTCCCACC TAAAGGTGGG TTCTGCTGCT GATATCCCCA TCAATATCTC AGAAACAGAC 
CTTAGCCTAC TCACAGCCAC TGTGGTGCCA CCTTCGGGTC GAGAGGAACC CTGTCTGCTG 
AAACGTTTGC GAAATGGCCA CGTGGGGATT TCCTTCGTGC CCAAGGAGAC AGGGGAGCAC 
CTGGTACATG TGAAGAAGAA TGGCCAGCAT GTGGCAAGCA GTCCCATCCC AGTAGTGATC 
AGCCAGTCGG AGATAGGTGA TGCCAGCCGT GTGAGGGTCT CTGGTCAAGG TCTTCATGAA 
GGTCATACCT TTGAGCCTGC AGAGTTTATT ATTGACACCA GAGATGCAGG CTACGGTGGG 
CTTAGTCTGT CCATTGAGGG CCCTAGCAAA GTAGACATCA ACACAGAGGA TCTGGAGGAT 
GGCACATGCA GGGTCACCTA CTGTCCCACA GAGCCTGGAA ACTACATTAT AAACATCAAA 
TTTGCTGACC AGCATGTGCC TGGCAGTCCC TTTTCTGTGA AGGTGACAGG TGAGGGCCGG 
GTGAAAGAGA GTATCACACG CAGGCGACGT GCCCCTTCTG TGGCCAATAT TGGCAGTCAT 
TGTGACCTCA GCCTGAAGAT TCCTGAAATT AGCATCCAAG ATATGACAGC CCAGGTGACC 
AGCCCATCAG GCAAGACCCA TGAGGCAGAG ATCGTAGAAG GAGAGAACCA TACTTACTGT 
ATCCGATTTG TGCCTGCTGA GATGGGAATG CATACAGTCA GTGTCAAGTA CAAGGGCCAG 
CATGTACCTG GGAGCCCCTT CCAGTTCACT GTGGGGCCTC TGGGGGAAGG GGGTGCTCAC 
AAGGTCCGTG CTGGAGGCCC TGGCCTAGAG AGGGCTGAAG TTGGAGTGCC AGCGGAGTTC 
GGCATTTGGA CTAGGGAAGC TGGCGCTGGA GGCCTGGCCA TTGCTGTTGA AGGCCCCAGC 
AAGGCTGAGA TCTCTTTCGA GGACCGAAAG GATGGCTCCT GTGGTGTGGC CTACGTAGTT 
CAGGAGCCAG GTGACTATGA GGTCTCAGTC AAGTTCAACG AGGAGCACAT ACCTGATAGC 
CCCTTCGTGG TGCCTGTGGC TTCTCCGTCT GGTGACGCCC GCCGCCTTAC TGTTTCTAGT 
CTTCAGGAGT CAGGGTTAAA GGTCAACCAG CCAGCATCTT TTGCAGTCAG TCTGAATGGA 
GCCAAGGGGG CAATTGATGC CAAGGTGCAC AGCCCCTCAG GAGCTCTGGA GGAGTGCTAT 
GTCACAGAGA TTGACCAAGA TAAGTATGCT GTGCGTTTCA TCCCACGAGA GAATGGCATC 
TACTTGATTG ATGTCAAGTT CAATGGTACT CACATTCCTG GAAGTCCCTT CAAGATCCGA 
GTTGGGGAGC CTGGGCATGG AGGGGACCCA GGCTTAGTGT CCGCCTATGG AGCAGGCCTG 
GAAGGTGGTG TCACAGGGAG CCCAGCAGAG TTTATTGTGA ACACAAGCAA TGCAGGAGCT 
GGTGCCCTTT CGGTTACCAT TGATGGCCCC TCCAAGGTGA AGATGGATTG CCAGGAGTGC 
CCCGAGGGCT ATCGTGTCAC CTATACCCCC ATGGCACCTG GCAGCTACCT CATCTCCATC 
AAGTATGGTG GCCCCTATCA CATTGGTGGA AGTCCCTTTA AAGCCAAGGT CACAGGTCCT 
CGTCTTGTAA GCAACCACAG CCTCCATGAG ACATCATCTG TGTTTGTGGA CTCTTTGACT 
AAAGTTGCCA CGGTTCCCCA GCATGCAACC TCAGGCCCAG GTCCTGCTGA TGTCAGCAAG 
GTAGTAGCCA AAGGCCTGGG GCTAAGCAAG GCTTATGTAG GCCAGAAGAG CAACTTCACA 
GTAGATTGCA GCAAAGCAGG TAACAACATG CTGCTGGTGG GCGTGCATGG CCCAAGGACA 
CCCTGTGAAG AGATCCTGGT GAAACACATG GGCAGCCGCC TCTATAGTGT CTCTTACCTG 
CTCAAGGACA AAGGGGAGTA CACACTGGTG GTCAAGTGGG GTGATGAGCA TATCCCAGGC 
AGCCCATACC GCATTATGGT ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Flna ( XM_017318433.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173922.1 Mus musculus filamin, alpha (Flna), transcript variant X3, mRNA.

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CloneID	OMu20537
Clone ID Related Accession (Same CDS sequence)	XM_017318433.1 , XM_017318433.2 , NM_001290421.1
Accession Version	XM_017318433.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	filamin-A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACA TCCGTGAGGC ACCCCAGGAT TTTCACCCAG ACAGGGTGAA GGCACGTGGG 
CCTGGATTGG AGAAGACTGG TGTGGCTGTC AACAAGCCAG CAGAGTTCAC AGTTGATGCC 
AAGCATGCTG GGAAGGCTCC TCTTCGAGTT CAAGTTCAGG ACAATGAGGG CTGCTCTGTG 
GAAGCGACAG TCAAGGACAA TGGCAATGGT ACTTACAGCT GTTCTTATGT GCCCAGAAAG 
CCAGTGAAGC ACACAGCCAT GGTTTCTTGG GGAGGTGTCA GCATCCCCAA CAGTCCTTTC 
CGGGTGAATG TGGGAGCTGG CAGCCATCCA AACAAAGTCA AGGTGTATGG TCCAGGAGTG 
GCCAAGACTG GGCTCAAGGC CCATGAACCT ACCTACTTTA CTGTGGATTG TACTGAAGCT 
GGCCAGGGAG ATGTCAGCAT TGGTATCAAG TGTGCCCCTG GAGTAGTGGG CCCCACTGAG 
GCTGATATTG ACTTTGATAT CATCCGCAAT GACAATGACA CCTTCACTGT AAAATACACA 
CCCTGTGGGG CTGGCAGCTA TACCATCATG GTCCTTTTTG CTGACCAGGC CACACCCACC 
AGCCCCATCA GAGTCAAAGT GGAGCCTTCT CATGATGCCA GCAAGGTGAA GGCTGAGGGT 
CCTGGCCTAA ATCGCACTGG TGTTGAACTT GGCAAACCCA CCCATTTCAC AGTCAATGCT 
AAAACTGCTG GGAAAGGCAA GCTGGATGTC CAATTCTCAG GACTGGCTAA GGGAGATGCA 
GTACGGGATG TGGACATCAT TGACCACCAT GATAATACCT ACACAGTCAA GTACATTCCT 
GTGCAGCAGG GCCCAGTAGG TGTCAATGTC ACTTATGGAG GAGATCACAT CCCCAAGAGT 
CCATTTTCAG TGGGAGTATC TCCAAGCCTG GATCTCAGCA AAATCAAGGT GTCTGGCCTT 
GGTGACAAAG TGGACGTTGG CAAAGATCAA GAGTTCACAG TAAAGTCAAA GGGTGCAGGT 
GGTCAAGGCA AAGTAGCATC CAAGATTGTG AGTCCCTCAG GTGCAGCGGT ACCCTGCAAG 
GTAGAGCCAG GCCTGGGAGC TGACAACAGC GTGGTACGTT TTGTGCCCCG TGAAGAGGGG 
CCCTATGAGG TGGAAGTGAC CTATGATGGT GTGCCTGTAC CTGGCAGTCC CTTTCCACTA 
GAAGCTGTGG CCCCCACCAA ACCCAGCAAG GTGAAGGCGT TTGGACCAGG GCTACAGGGG 
GGCAATGCAG GCTCCCCTGC CCGCTTCACC ATTGATACAA AGGGTGCTGG CACTGGTGGC 
CTGGGCCTGA CAGTGGAAGG CCCCTGTGAA GCACAGCTTG AGTGCCTAGA CAACGGGGAT 
GGTACATGCT CTGTGTCTTA TGTACCCACT GAGCCTGGGG ACTACAACAT CAACATCCTT 
TTTGCTGACA CCCACATTCC TGGATCCCCA TTCAAGGCCC ATGTGGCTCC TTGTTTTGAT 
GCATCCAAGG TGAAGTGCTC AGGCCCTGGG CTGGAGCGGG CTACTGCTGG TGAGGTAGGG 
CAGTTCCAAG TGGACTGTTC AAGTGCTGGC AGTGCTGAGT TGACGATTGA GATCTGCTCT 
GAGGCAGGAC TGCCAGCTGA AGTATACATT CAAGACCATG GTGATGGCAC ACACACCATT 
ACCTATATTC CTCTCTGTCC TGGGGCTTAC ACTGTTACCA TCAAGTATGG CGGCCAGCCT 
GTGCCCAACT TCCCCAGCAA GCTACAGGTG GAACCTGCTG TAGATACCTC AGGTGTACAG 
TGCTATGGGC CTGGGATTGA AGGTCAAGGT GTCTTCCGAG AGGCAACCAC TGAGTTCAGT 
GTGGATGCCC GGGCTCTTAC ACAGACTGGA GGGCCACATG TCAAGGCTCG AGTGGCCAAC 
CCCTCAGGCA ATCTGACAGA TACCTATGTG CAAGACTGTG GGGATGGCAC ATACAAAGTG 
GAATACACTC CATATGAGGA AGGAGTACAC TCTGTGGATG TGACTTATGA TGGCAGCCCT 
GTGCCCAGCA GCCCCTTCCA GGTGCCTGTA ACAGAGGGCT GTGACCCCTC CCGGGTGCGT 
GTCCATGGAC CAGGCATCCA AAGTGGTACC ACCAACAAAC CCAACAAGTT CACAGTAGAA 
ACCAGGGGAG CTGGCACAGG TGGCCTGGGC TTGGCTGTTG AGGGTCCCTC AGAGGCCAAG 
ATGTCTTGTA TGGATAATAA AGATGGCAGC TGCTCAGTAG AATACATCCC CTATGAAGCT 
GGAACCTATA GCCTTAATGT CACTTATGGT GGTCACCAAG TGCCAGGTAG TCCCTTCAAG 
GTCCCTGTAC ATGATGTGAC AGATGCATCT AAAGTCAAGT GTTCTGGACC TGGCCTAAGC 
CCAGGCATGG TCCGTGCCAA CCTCCCTCAG TCCTTTCAGG TGGACACAAG CAAAGCTGGA 
GTTGCCCCAC TGCAGGTCAA AGTGCAGGGG CCCAAAGGCC TGGTGGAGCC AGTGGATGTA 
GTGGACAATG CTGATGGTAC TCAGACTGTC AACTATGTGC CCAGCCGAGA AGGGTCCTAT 
AGCATTTCTG TGCTGTATGG TGAAGAAGAA GTGCCACGGA GCCCCTTCAA GGTCAAGGTG 
CTGCCTACAC ATGATGCCAG TAAGGTGAAG GCCAGTGGAC CTGGACTCAA CACCACTGGT 
GTACCTGCTA GCCTGCCTGT GGAGTTCACC ATTGATGCCA AGGATGCTGG GGAGGGTCTG 
TTGGCTGTCC AGATTACGGA TCCTGAAGGC AAGCCCAAGA AGACACACAT TCAAGATAAT 
CATGATGGCA CATACACGGT GGCTTATGTG CCAGATGTGC CAGGCCGGTA CACAATCCTC 
ATCAAGTATG GTGGTGATGA GATTCCCTTT TCCCCGTACC GTGTCCGGGC TGTGCCCACT 
GGGGATGCCA GCAAGTGCAC AGTCACAGTG TCAATCGGAG GTCACGGGCT AGGTGCTGGC 
ATTGGCCCCA CCATCCAGAT TGGGGAGGAG ACGGTGATTA CTGTGGACAC AAAAGCAGCA 
GGCAAAGGCA AAGTGACTTG TACTGTGTGC ACACCTGATG GCTCAGAGGT AGACGTGGAC 
GTGGTGGAGA ATGAGGATGG CACCTTTGAC ATCTTCTACA CAGCTCCCCA ACCGGGCAAA 
TATGTCATCT GTGTGCGCTT CGGTGGCGAG CATGTGCCCA ACAGCCCCTT CCAAGTTACA 
GCTTTGGCTG GGGACCAACC AACAGTGCAG ACCCCATTAA GGTCTCAGCA GCTGGCTCCA 
CAGTATAACT ATCCTCAGGG TAGCCAGCAA ACCTGGATTC CAGAGAGGCC CATGGTGGGC 
GTTAATGGGC TGGATGTGAC CAGCCTGAGG CCCTTTGATC TTGTCATCCC CTTCACTATC 
AAGAAGGGGG AGATCACTGG GGAAGTTCGA ATGCCCTCAG GCAAGGTGGC CCAGCCTTCC 
ATTACTGATA ACAAAGATGG CACTGTTACT GTACGTTACT CACCCAGTGA AGCTGGCCTG 
CATGAAATGG ACATTCGCTA TGACAATATG CATATCCCAG GAAGCCCTCT GCAGTTCTAT 
GTTGATTATG TCAACTGTGG CCACATCACT GCTTATGGTC CTGGCCTTAC CCATGGAGTG 
GTCAACAAAC CTGCCACCTT CACTGTCAAC ACCAAGGATG CAGGAGAGGG GGGCTTGTCT 
CTGGCCATTG AGGGTCCATC TAAAGCAGAA ATCAGTTGCA CTGACAACCA GGATGGAACA 
TGCAGTGTCT CTTACCTGCC TGTACTGCCT GGTGACTATA GCATCCTAGT TAAGTACAAT 
GATCAACACA TCCCAGGCAG TCCCTTTACT GCCAGAGTAA CAGGTGACGA TTCCATGCGT 
ATGTCCCACC TAAAGGTGGG TTCTGCTGCT GATATCCCCA TCAATATCTC AGAAACAGAC 
CTTAGCCTAC TCACAGCCAC TGTGGTGCCA CCTTCGGGTC GAGAGGAACC CTGTCTGCTG 
AAACGTTTGC GAAATGGCCA CGTGGGGATT TCCTTCGTGC CCAAGGAGAC AGGGGAGCAC 
CTGGTACATG TGAAGAAGAA TGGCCAGCAT GTGGCAAGCA GTCCCATCCC AGTAGTGATC 
AGCCAGTCGG AGATAGGTGA TGCCAGCCGT GTGAGGGTCT CTGGTCAAGG TCTTCATGAA 
GGTCATACCT TTGAGCCTGC AGAGTTTATT ATTGACACCA GAGATGCAGG CTACGGTGGG 
CTTAGTCTGT CCATTGAGGG CCCTAGCAAA GTAGACATCA ACACAGAGGA TCTGGAGGAT 
GGCACATGCA GGGTCACCTA CTGTCCCACA GAGCCTGGAA ACTACATTAT AAACATCAAA 
TTTGCTGACC AGCATGTGCC TGGCAGTCCC TTTTCTGTGA AGGTGACAGG TGAGGGCCGG 
GTGAAAGAGA GTATCACACG CAGGCGACGT GCCCCTTCTG TGGCCAATAT TGGCAGTCAT 
TGTGACCTCA GCCTGAAGAT TCCTGAAATT AGCATCCAAG ATATGACAGC CCAGGTGACC 
AGCCCATCAG GCAAGACCCA TGAGGCAGAG ATCGTAGAAG GAGAGAACCA TACTTACTGT 
ATCCGATTTG TGCCTGCTGA GATGGGAATG CATACAGTCA GTGTCAAGTA CAAGGGCCAG 
CATGTACCTG GGAGCCCCTT CCAGTTCACT GTGGGGCCTC TGGGGGAAGG GGGTGCTCAC 
AAGGTCCGTG CTGGAGGCCC TGGCCTAGAG AGGGCTGAAG TTGGAGTGCC AGCGGAGTTC 
GGCATTTGGA CTAGGGAAGC TGGCGCTGGA GGCCTGGCCA TTGCTGTTGA AGGCCCCAGC 
AAGGCTGAGA TCTCTTTCGA GGACCGAAAG GATGGCTCCT GTGGTGTGGC CTACGTAGTT 
CAGGAGCCAG GTGACTATGA GGTCTCAGTC AAGTTCAACG AGGAGCACAT ACCTGATAGC 
CCCTTCGTGG TGCCTGTGGC TTCTCCGTCT GGTGACGCCC GCCGCCTTAC TGTTTCTAGT 
CTTCAGGAGT CAGGGTTAAA GGTCAACCAG CCAGCATCTT TTGCAGTCAG TCTGAATGGA 
GCCAAGGGGG CAATTGATGC CAAGGTGCAC AGCCCCTCAG GAGCTCTGGA GGAGTGCTAT 
GTCACAGAGA TTGACCAAGA TAAGTATGCT GTGCGTTTCA TCCCACGAGA GAATGGCATC 
TACTTGATTG ATGTCAAGTT CAATGGTACT CACATTCCTG GAAGTCCCTT CAAGATCCGA 
GTTGGGGAGC CTGGGCATGG AGGGGACCCA GGCTTAGTGT CCGCCTATGG AGCAGGCCTG 
GAAGGTGGTG TCACAGGGAG CCCAGCAGAG TTTATTGTGA ACACAAGCAA TGCAGGAGCT 
GGTGCCCTTT CGGTTACCAT TGATGGCCCC TCCAAGGTGA AGATGGATTG CCAGGAGTGC 
CCCGAGGGCT ATCGTGTCAC CTATACCCCC ATGGCACCTG GCAGCTACCT CATCTCCATC 
AAGTATGGTG GCCCCTATCA CATTGGTGGA AGTCCCTTTA AAGCCAAGGT CACAGGTCCT 
CGTCTTGTAA GCAACCACAG CCTCCATGAG ACATCATCTG TGTTTGTGGA CTCTTTGACT 
AAAGTTGCCA CGGTTCCCCA GCATGCAACC TCAGGCCCAG GTCCTGCTGA TGTCAGCAAG 
GTAGTAGCCA AAGGCCTGGG GCTAAGCAAG GCTTATGTAG GCCAGAAGAG CAACTTCACA 
GTAGATTGCA GCAAAGCAGG TAACAACATG CTGCTGGTGG GCGTGCATGG CCCAAGGACA 
CCCTGTGAAG AGATCCTGGT GAAACACATG GGCAGCCGCC TCTATAGTGT CTCTTACCTG 
CTCAAGGACA AAGGGGAGTA CACACTGGTG GTCAAGTGGG GTGATGAGCA TATCCCAGGC 
AGCCCATACC GCATTATGGT ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173922.1
CDS	1834..7800
Translation	MADIREAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAKHAGKAPLRVQVQDNEGCSVEATVKDNGNGTYSCSYVPRKPVKHTAMVSWGGVSIPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCTEAGQGDVSIGIKCAPGVVGPTEADIDFDIIRNDNDTFTVKYTPCGAGSYTIMVLFADQATPTSPIRVKVEPSHDASKVKAEGPGLNRTGVELGKPTHFTVNAKTAGKGKLDVQFSGLAKGDAVRDVDIIDHHDNTYTVKYIPVQQGPVGVNVTYGGDHIPKSPFSVGVSPSLDLSKIKVSGLGDKVDVGKDQEFTVKSKGAGGQGKVASKIVSPSGAAVPCKVEPGLGADNSVVRFVPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQGGNAGSPARFTIDTKGAGTGGLGLTVEGPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVAPCFDASKVKCSGPGLERATAGEVGQFQVDCSSAGSAELTIEICSEAGLPAEVYIQDHGDGTHTITYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTGGPHVKARVANPSGNLTDTYVQDCGDGTYKVEYTPYEEGVHSVDVTYDGSPVPSSPFQVPVTEGCDPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKMSCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDVVDNADGTQTVNYVPSREGSYSISVLYGEEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVPDVPGRYTILIKYGGDEIPFSPYRVRAVPTGDASKCTVTVSIGGHGLGAGIGPTIQIGEETVITVDTKAAGKGKVTCTVCTPDGSEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPTVQTPLRSQQLAPQYNYPQGSQQTWIPERPMVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPSITDNKDGTVTVRYSPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHITAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNDQHIPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANIGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGMHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEVGVPAEFGIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGIYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGSPAEFIVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKVATVPQHATSGPGPADVSKVVAKGLGLSKAYVGQKSNFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHMGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRIMVP

Target ORF information:

RefSeq Version	XM_017318433.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus filamin, alpha (Flna), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318433.1

ORF Insert Sequence:

ATGGCTGACA TCCGTGAGGC ACCCCAGGAT TTTCACCCAG ACAGGGTGAA GGCACGTGGG 
CCTGGATTGG AGAAGACTGG TGTGGCTGTC AACAAGCCAG CAGAGTTCAC AGTTGATGCC 
AAGCATGCTG GGAAGGCTCC TCTTCGAGTT CAAGTTCAGG ACAATGAGGG CTGCTCTGTG 
GAAGCGACAG TCAAGGACAA TGGCAATGGT ACTTACAGCT GTTCTTATGT GCCCAGAAAG 
CCAGTGAAGC ACACAGCCAT GGTTTCTTGG GGAGGTGTCA GCATCCCCAA CAGTCCTTTC 
CGGGTGAATG TGGGAGCTGG CAGCCATCCA AACAAAGTCA AGGTGTATGG TCCAGGAGTG 
GCCAAGACTG GGCTCAAGGC CCATGAACCT ACCTACTTTA CTGTGGATTG TACTGAAGCT 
GGCCAGGGAG ATGTCAGCAT TGGTATCAAG TGTGCCCCTG GAGTAGTGGG CCCCACTGAG 
GCTGATATTG ACTTTGATAT CATCCGCAAT GACAATGACA CCTTCACTGT AAAATACACA 
CCCTGTGGGG CTGGCAGCTA TACCATCATG GTCCTTTTTG CTGACCAGGC CACACCCACC 
AGCCCCATCA GAGTCAAAGT GGAGCCTTCT CATGATGCCA GCAAGGTGAA GGCTGAGGGT 
CCTGGCCTAA ATCGCACTGG TGTTGAACTT GGCAAACCCA CCCATTTCAC AGTCAATGCT 
AAAACTGCTG GGAAAGGCAA GCTGGATGTC CAATTCTCAG GACTGGCTAA GGGAGATGCA 
GTACGGGATG TGGACATCAT TGACCACCAT GATAATACCT ACACAGTCAA GTACATTCCT 
GTGCAGCAGG GCCCAGTAGG TGTCAATGTC ACTTATGGAG GAGATCACAT CCCCAAGAGT 
CCATTTTCAG TGGGAGTATC TCCAAGCCTG GATCTCAGCA AAATCAAGGT GTCTGGCCTT 
GGTGACAAAG TGGACGTTGG CAAAGATCAA GAGTTCACAG TAAAGTCAAA GGGTGCAGGT 
GGTCAAGGCA AAGTAGCATC CAAGATTGTG AGTCCCTCAG GTGCAGCGGT ACCCTGCAAG 
GTAGAGCCAG GCCTGGGAGC TGACAACAGC GTGGTACGTT TTGTGCCCCG TGAAGAGGGG 
CCCTATGAGG TGGAAGTGAC CTATGATGGT GTGCCTGTAC CTGGCAGTCC CTTTCCACTA 
GAAGCTGTGG CCCCCACCAA ACCCAGCAAG GTGAAGGCGT TTGGACCAGG GCTACAGGGG 
GGCAATGCAG GCTCCCCTGC CCGCTTCACC ATTGATACAA AGGGTGCTGG CACTGGTGGC 
CTGGGCCTGA CAGTGGAAGG CCCCTGTGAA GCACAGCTTG AGTGCCTAGA CAACGGGGAT 
GGTACATGCT CTGTGTCTTA TGTACCCACT GAGCCTGGGG ACTACAACAT CAACATCCTT 
TTTGCTGACA CCCACATTCC TGGATCCCCA TTCAAGGCCC ATGTGGCTCC TTGTTTTGAT 
GCATCCAAGG TGAAGTGCTC AGGCCCTGGG CTGGAGCGGG CTACTGCTGG TGAGGTAGGG 
CAGTTCCAAG TGGACTGTTC AAGTGCTGGC AGTGCTGAGT TGACGATTGA GATCTGCTCT 
GAGGCAGGAC TGCCAGCTGA AGTATACATT CAAGACCATG GTGATGGCAC ACACACCATT 
ACCTATATTC CTCTCTGTCC TGGGGCTTAC ACTGTTACCA TCAAGTATGG CGGCCAGCCT 
GTGCCCAACT TCCCCAGCAA GCTACAGGTG GAACCTGCTG TAGATACCTC AGGTGTACAG 
TGCTATGGGC CTGGGATTGA AGGTCAAGGT GTCTTCCGAG AGGCAACCAC TGAGTTCAGT 
GTGGATGCCC GGGCTCTTAC ACAGACTGGA GGGCCACATG TCAAGGCTCG AGTGGCCAAC 
CCCTCAGGCA ATCTGACAGA TACCTATGTG CAAGACTGTG GGGATGGCAC ATACAAAGTG 
GAATACACTC CATATGAGGA AGGAGTACAC TCTGTGGATG TGACTTATGA TGGCAGCCCT 
GTGCCCAGCA GCCCCTTCCA GGTGCCTGTA ACAGAGGGCT GTGACCCCTC CCGGGTGCGT 
GTCCATGGAC CAGGCATCCA AAGTGGTACC ACCAACAAAC CCAACAAGTT CACAGTAGAA 
ACCAGGGGAG CTGGCACAGG TGGCCTGGGC TTGGCTGTTG AGGGTCCCTC AGAGGCCAAG 
ATGTCTTGTA TGGATAATAA AGATGGCAGC TGCTCAGTAG AATACATCCC CTATGAAGCT 
GGAACCTATA GCCTTAATGT CACTTATGGT GGTCACCAAG TGCCAGGTAG TCCCTTCAAG 
GTCCCTGTAC ATGATGTGAC AGATGCATCT AAAGTCAAGT GTTCTGGACC TGGCCTAAGC 
CCAGGCATGG TCCGTGCCAA CCTCCCTCAG TCCTTTCAGG TGGACACAAG CAAAGCTGGA 
GTTGCCCCAC TGCAGGTCAA AGTGCAGGGG CCCAAAGGCC TGGTGGAGCC AGTGGATGTA 
GTGGACAATG CTGATGGTAC TCAGACTGTC AACTATGTGC CCAGCCGAGA AGGGTCCTAT 
AGCATTTCTG TGCTGTATGG TGAAGAAGAA GTGCCACGGA GCCCCTTCAA GGTCAAGGTG 
CTGCCTACAC ATGATGCCAG TAAGGTGAAG GCCAGTGGAC CTGGACTCAA CACCACTGGT 
GTACCTGCTA GCCTGCCTGT GGAGTTCACC ATTGATGCCA AGGATGCTGG GGAGGGTCTG 
TTGGCTGTCC AGATTACGGA TCCTGAAGGC AAGCCCAAGA AGACACACAT TCAAGATAAT 
CATGATGGCA CATACACGGT GGCTTATGTG CCAGATGTGC CAGGCCGGTA CACAATCCTC 
ATCAAGTATG GTGGTGATGA GATTCCCTTT TCCCCGTACC GTGTCCGGGC TGTGCCCACT 
GGGGATGCCA GCAAGTGCAC AGTCACAGTG TCAATCGGAG GTCACGGGCT AGGTGCTGGC 
ATTGGCCCCA CCATCCAGAT TGGGGAGGAG ACGGTGATTA CTGTGGACAC AAAAGCAGCA 
GGCAAAGGCA AAGTGACTTG TACTGTGTGC ACACCTGATG GCTCAGAGGT AGACGTGGAC 
GTGGTGGAGA ATGAGGATGG CACCTTTGAC ATCTTCTACA CAGCTCCCCA ACCGGGCAAA 
TATGTCATCT GTGTGCGCTT CGGTGGCGAG CATGTGCCCA ACAGCCCCTT CCAAGTTACA 
GCTTTGGCTG GGGACCAACC AACAGTGCAG ACCCCATTAA GGTCTCAGCA GCTGGCTCCA 
CAGTATAACT ATCCTCAGGG TAGCCAGCAA ACCTGGATTC CAGAGAGGCC CATGGTGGGC 
GTTAATGGGC TGGATGTGAC CAGCCTGAGG CCCTTTGATC TTGTCATCCC CTTCACTATC 
AAGAAGGGGG AGATCACTGG GGAAGTTCGA ATGCCCTCAG GCAAGGTGGC CCAGCCTTCC 
ATTACTGATA ACAAAGATGG CACTGTTACT GTACGTTACT CACCCAGTGA AGCTGGCCTG 
CATGAAATGG ACATTCGCTA TGACAATATG CATATCCCAG GAAGCCCTCT GCAGTTCTAT 
GTTGATTATG TCAACTGTGG CCACATCACT GCTTATGGTC CTGGCCTTAC CCATGGAGTG 
GTCAACAAAC CTGCCACCTT CACTGTCAAC ACCAAGGATG CAGGAGAGGG GGGCTTGTCT 
CTGGCCATTG AGGGTCCATC TAAAGCAGAA ATCAGTTGCA CTGACAACCA GGATGGAACA 
TGCAGTGTCT CTTACCTGCC TGTACTGCCT GGTGACTATA GCATCCTAGT TAAGTACAAT 
GATCAACACA TCCCAGGCAG TCCCTTTACT GCCAGAGTAA CAGGTGACGA TTCCATGCGT 
ATGTCCCACC TAAAGGTGGG TTCTGCTGCT GATATCCCCA TCAATATCTC AGAAACAGAC 
CTTAGCCTAC TCACAGCCAC TGTGGTGCCA CCTTCGGGTC GAGAGGAACC CTGTCTGCTG 
AAACGTTTGC GAAATGGCCA CGTGGGGATT TCCTTCGTGC CCAAGGAGAC AGGGGAGCAC 
CTGGTACATG TGAAGAAGAA TGGCCAGCAT GTGGCAAGCA GTCCCATCCC AGTAGTGATC 
AGCCAGTCGG AGATAGGTGA TGCCAGCCGT GTGAGGGTCT CTGGTCAAGG TCTTCATGAA 
GGTCATACCT TTGAGCCTGC AGAGTTTATT ATTGACACCA GAGATGCAGG CTACGGTGGG 
CTTAGTCTGT CCATTGAGGG CCCTAGCAAA GTAGACATCA ACACAGAGGA TCTGGAGGAT 
GGCACATGCA GGGTCACCTA CTGTCCCACA GAGCCTGGAA ACTACATTAT AAACATCAAA 
TTTGCTGACC AGCATGTGCC TGGCAGTCCC TTTTCTGTGA AGGTGACAGG TGAGGGCCGG 
GTGAAAGAGA GTATCACACG CAGGCGACGT GCCCCTTCTG TGGCCAATAT TGGCAGTCAT 
TGTGACCTCA GCCTGAAGAT TCCTGAAATT AGCATCCAAG ATATGACAGC CCAGGTGACC 
AGCCCATCAG GCAAGACCCA TGAGGCAGAG ATCGTAGAAG GAGAGAACCA TACTTACTGT 
ATCCGATTTG TGCCTGCTGA GATGGGAATG CATACAGTCA GTGTCAAGTA CAAGGGCCAG 
CATGTACCTG GGAGCCCCTT CCAGTTCACT GTGGGGCCTC TGGGGGAAGG GGGTGCTCAC 
AAGGTCCGTG CTGGAGGCCC TGGCCTAGAG AGGGCTGAAG TTGGAGTGCC AGCGGAGTTC 
GGCATTTGGA CTAGGGAAGC TGGCGCTGGA GGCCTGGCCA TTGCTGTTGA AGGCCCCAGC 
AAGGCTGAGA TCTCTTTCGA GGACCGAAAG GATGGCTCCT GTGGTGTGGC CTACGTAGTT 
CAGGAGCCAG GTGACTATGA GGTCTCAGTC AAGTTCAACG AGGAGCACAT ACCTGATAGC 
CCCTTCGTGG TGCCTGTGGC TTCTCCGTCT GGTGACGCCC GCCGCCTTAC TGTTTCTAGT 
CTTCAGGAGT CAGGGTTAAA GGTCAACCAG CCAGCATCTT TTGCAGTCAG TCTGAATGGA 
GCCAAGGGGG CAATTGATGC CAAGGTGCAC AGCCCCTCAG GAGCTCTGGA GGAGTGCTAT 
GTCACAGAGA TTGACCAAGA TAAGTATGCT GTGCGTTTCA TCCCACGAGA GAATGGCATC 
TACTTGATTG ATGTCAAGTT CAATGGTACT CACATTCCTG GAAGTCCCTT CAAGATCCGA 
GTTGGGGAGC CTGGGCATGG AGGGGACCCA GGCTTAGTGT CCGCCTATGG AGCAGGCCTG 
GAAGGTGGTG TCACAGGGAG CCCAGCAGAG TTTATTGTGA ACACAAGCAA TGCAGGAGCT 
GGTGCCCTTT CGGTTACCAT TGATGGCCCC TCCAAGGTGA AGATGGATTG CCAGGAGTGC 
CCCGAGGGCT ATCGTGTCAC CTATACCCCC ATGGCACCTG GCAGCTACCT CATCTCCATC 
AAGTATGGTG GCCCCTATCA CATTGGTGGA AGTCCCTTTA AAGCCAAGGT CACAGGTCCT 
CGTCTTGTAA GCAACCACAG CCTCCATGAG ACATCATCTG TGTTTGTGGA CTCTTTGACT 
AAAGTTGCCA CGGTTCCCCA GCATGCAACC TCAGGCCCAG GTCCTGCTGA TGTCAGCAAG 
GTAGTAGCCA AAGGCCTGGG GCTAAGCAAG GCTTATGTAG GCCAGAAGAG CAACTTCACA 
GTAGATTGCA GCAAAGCAGG TAACAACATG CTGCTGGTGG GCGTGCATGG CCCAAGGACA 
CCCTGTGAAG AGATCCTGGT GAAACACATG GGCAGCCGCC TCTATAGTGT CTCTTACCTG 
CTCAAGGACA AAGGGGAGTA CACACTGGTG GTCAAGTGGG GTGATGAGCA TATCCCAGGC 
AGCCCATACC GCATTATGGT ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Flna ( XM_017318433.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173922.1 Mus musculus filamin, alpha (Flna), transcript variant X3, mRNA.

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CloneID	OMu20537
Clone ID Related Accession (Same CDS sequence)	XM_017318433.1 , XM_017318433.2 , NM_001290421.1
Accession Version	XM_017318433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	filamin-A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318433.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACA TCCGTGAGGC ACCCCAGGAT TTTCACCCAG ACAGGGTGAA GGCACGTGGG 
CCTGGATTGG AGAAGACTGG TGTGGCTGTC AACAAGCCAG CAGAGTTCAC AGTTGATGCC 
AAGCATGCTG GGAAGGCTCC TCTTCGAGTT CAAGTTCAGG ACAATGAGGG CTGCTCTGTG 
GAAGCGACAG TCAAGGACAA TGGCAATGGT ACTTACAGCT GTTCTTATGT GCCCAGAAAG 
CCAGTGAAGC ACACAGCCAT GGTTTCTTGG GGAGGTGTCA GCATCCCCAA CAGTCCTTTC 
CGGGTGAATG TGGGAGCTGG CAGCCATCCA AACAAAGTCA AGGTGTATGG TCCAGGAGTG 
GCCAAGACTG GGCTCAAGGC CCATGAACCT ACCTACTTTA CTGTGGATTG TACTGAAGCT 
GGCCAGGGAG ATGTCAGCAT TGGTATCAAG TGTGCCCCTG GAGTAGTGGG CCCCACTGAG 
GCTGATATTG ACTTTGATAT CATCCGCAAT GACAATGACA CCTTCACTGT AAAATACACA 
CCCTGTGGGG CTGGCAGCTA TACCATCATG GTCCTTTTTG CTGACCAGGC CACACCCACC 
AGCCCCATCA GAGTCAAAGT GGAGCCTTCT CATGATGCCA GCAAGGTGAA GGCTGAGGGT 
CCTGGCCTAA ATCGCACTGG TGTTGAACTT GGCAAACCCA CCCATTTCAC AGTCAATGCT 
AAAACTGCTG GGAAAGGCAA GCTGGATGTC CAATTCTCAG GACTGGCTAA GGGAGATGCA 
GTACGGGATG TGGACATCAT TGACCACCAT GATAATACCT ACACAGTCAA GTACATTCCT 
GTGCAGCAGG GCCCAGTAGG TGTCAATGTC ACTTATGGAG GAGATCACAT CCCCAAGAGT 
CCATTTTCAG TGGGAGTATC TCCAAGCCTG GATCTCAGCA AAATCAAGGT GTCTGGCCTT 
GGTGACAAAG TGGACGTTGG CAAAGATCAA GAGTTCACAG TAAAGTCAAA GGGTGCAGGT 
GGTCAAGGCA AAGTAGCATC CAAGATTGTG AGTCCCTCAG GTGCAGCGGT ACCCTGCAAG 
GTAGAGCCAG GCCTGGGAGC TGACAACAGC GTGGTACGTT TTGTGCCCCG TGAAGAGGGG 
CCCTATGAGG TGGAAGTGAC CTATGATGGT GTGCCTGTAC CTGGCAGTCC CTTTCCACTA 
GAAGCTGTGG CCCCCACCAA ACCCAGCAAG GTGAAGGCGT TTGGACCAGG GCTACAGGGG 
GGCAATGCAG GCTCCCCTGC CCGCTTCACC ATTGATACAA AGGGTGCTGG CACTGGTGGC 
CTGGGCCTGA CAGTGGAAGG CCCCTGTGAA GCACAGCTTG AGTGCCTAGA CAACGGGGAT 
GGTACATGCT CTGTGTCTTA TGTACCCACT GAGCCTGGGG ACTACAACAT CAACATCCTT 
TTTGCTGACA CCCACATTCC TGGATCCCCA TTCAAGGCCC ATGTGGCTCC TTGTTTTGAT 
GCATCCAAGG TGAAGTGCTC AGGCCCTGGG CTGGAGCGGG CTACTGCTGG TGAGGTAGGG 
CAGTTCCAAG TGGACTGTTC AAGTGCTGGC AGTGCTGAGT TGACGATTGA GATCTGCTCT 
GAGGCAGGAC TGCCAGCTGA AGTATACATT CAAGACCATG GTGATGGCAC ACACACCATT 
ACCTATATTC CTCTCTGTCC TGGGGCTTAC ACTGTTACCA TCAAGTATGG CGGCCAGCCT 
GTGCCCAACT TCCCCAGCAA GCTACAGGTG GAACCTGCTG TAGATACCTC AGGTGTACAG 
TGCTATGGGC CTGGGATTGA AGGTCAAGGT GTCTTCCGAG AGGCAACCAC TGAGTTCAGT 
GTGGATGCCC GGGCTCTTAC ACAGACTGGA GGGCCACATG TCAAGGCTCG AGTGGCCAAC 
CCCTCAGGCA ATCTGACAGA TACCTATGTG CAAGACTGTG GGGATGGCAC ATACAAAGTG 
GAATACACTC CATATGAGGA AGGAGTACAC TCTGTGGATG TGACTTATGA TGGCAGCCCT 
GTGCCCAGCA GCCCCTTCCA GGTGCCTGTA ACAGAGGGCT GTGACCCCTC CCGGGTGCGT 
GTCCATGGAC CAGGCATCCA AAGTGGTACC ACCAACAAAC CCAACAAGTT CACAGTAGAA 
ACCAGGGGAG CTGGCACAGG TGGCCTGGGC TTGGCTGTTG AGGGTCCCTC AGAGGCCAAG 
ATGTCTTGTA TGGATAATAA AGATGGCAGC TGCTCAGTAG AATACATCCC CTATGAAGCT 
GGAACCTATA GCCTTAATGT CACTTATGGT GGTCACCAAG TGCCAGGTAG TCCCTTCAAG 
GTCCCTGTAC ATGATGTGAC AGATGCATCT AAAGTCAAGT GTTCTGGACC TGGCCTAAGC 
CCAGGCATGG TCCGTGCCAA CCTCCCTCAG TCCTTTCAGG TGGACACAAG CAAAGCTGGA 
GTTGCCCCAC TGCAGGTCAA AGTGCAGGGG CCCAAAGGCC TGGTGGAGCC AGTGGATGTA 
GTGGACAATG CTGATGGTAC TCAGACTGTC AACTATGTGC CCAGCCGAGA AGGGTCCTAT 
AGCATTTCTG TGCTGTATGG TGAAGAAGAA GTGCCACGGA GCCCCTTCAA GGTCAAGGTG 
CTGCCTACAC ATGATGCCAG TAAGGTGAAG GCCAGTGGAC CTGGACTCAA CACCACTGGT 
GTACCTGCTA GCCTGCCTGT GGAGTTCACC ATTGATGCCA AGGATGCTGG GGAGGGTCTG 
TTGGCTGTCC AGATTACGGA TCCTGAAGGC AAGCCCAAGA AGACACACAT TCAAGATAAT 
CATGATGGCA CATACACGGT GGCTTATGTG CCAGATGTGC CAGGCCGGTA CACAATCCTC 
ATCAAGTATG GTGGTGATGA GATTCCCTTT TCCCCGTACC GTGTCCGGGC TGTGCCCACT 
GGGGATGCCA GCAAGTGCAC AGTCACAGTG TCAATCGGAG GTCACGGGCT AGGTGCTGGC 
ATTGGCCCCA CCATCCAGAT TGGGGAGGAG ACGGTGATTA CTGTGGACAC AAAAGCAGCA 
GGCAAAGGCA AAGTGACTTG TACTGTGTGC ACACCTGATG GCTCAGAGGT AGACGTGGAC 
GTGGTGGAGA ATGAGGATGG CACCTTTGAC ATCTTCTACA CAGCTCCCCA ACCGGGCAAA 
TATGTCATCT GTGTGCGCTT CGGTGGCGAG CATGTGCCCA ACAGCCCCTT CCAAGTTACA 
GCTTTGGCTG GGGACCAACC AACAGTGCAG ACCCCATTAA GGTCTCAGCA GCTGGCTCCA 
CAGTATAACT ATCCTCAGGG TAGCCAGCAA ACCTGGATTC CAGAGAGGCC CATGGTGGGC 
GTTAATGGGC TGGATGTGAC CAGCCTGAGG CCCTTTGATC TTGTCATCCC CTTCACTATC 
AAGAAGGGGG AGATCACTGG GGAAGTTCGA ATGCCCTCAG GCAAGGTGGC CCAGCCTTCC 
ATTACTGATA ACAAAGATGG CACTGTTACT GTACGTTACT CACCCAGTGA AGCTGGCCTG 
CATGAAATGG ACATTCGCTA TGACAATATG CATATCCCAG GAAGCCCTCT GCAGTTCTAT 
GTTGATTATG TCAACTGTGG CCACATCACT GCTTATGGTC CTGGCCTTAC CCATGGAGTG 
GTCAACAAAC CTGCCACCTT CACTGTCAAC ACCAAGGATG CAGGAGAGGG GGGCTTGTCT 
CTGGCCATTG AGGGTCCATC TAAAGCAGAA ATCAGTTGCA CTGACAACCA GGATGGAACA 
TGCAGTGTCT CTTACCTGCC TGTACTGCCT GGTGACTATA GCATCCTAGT TAAGTACAAT 
GATCAACACA TCCCAGGCAG TCCCTTTACT GCCAGAGTAA CAGGTGACGA TTCCATGCGT 
ATGTCCCACC TAAAGGTGGG TTCTGCTGCT GATATCCCCA TCAATATCTC AGAAACAGAC 
CTTAGCCTAC TCACAGCCAC TGTGGTGCCA CCTTCGGGTC GAGAGGAACC CTGTCTGCTG 
AAACGTTTGC GAAATGGCCA CGTGGGGATT TCCTTCGTGC CCAAGGAGAC AGGGGAGCAC 
CTGGTACATG TGAAGAAGAA TGGCCAGCAT GTGGCAAGCA GTCCCATCCC AGTAGTGATC 
AGCCAGTCGG AGATAGGTGA TGCCAGCCGT GTGAGGGTCT CTGGTCAAGG TCTTCATGAA 
GGTCATACCT TTGAGCCTGC AGAGTTTATT ATTGACACCA GAGATGCAGG CTACGGTGGG 
CTTAGTCTGT CCATTGAGGG CCCTAGCAAA GTAGACATCA ACACAGAGGA TCTGGAGGAT 
GGCACATGCA GGGTCACCTA CTGTCCCACA GAGCCTGGAA ACTACATTAT AAACATCAAA 
TTTGCTGACC AGCATGTGCC TGGCAGTCCC TTTTCTGTGA AGGTGACAGG TGAGGGCCGG 
GTGAAAGAGA GTATCACACG CAGGCGACGT GCCCCTTCTG TGGCCAATAT TGGCAGTCAT 
TGTGACCTCA GCCTGAAGAT TCCTGAAATT AGCATCCAAG ATATGACAGC CCAGGTGACC 
AGCCCATCAG GCAAGACCCA TGAGGCAGAG ATCGTAGAAG GAGAGAACCA TACTTACTGT 
ATCCGATTTG TGCCTGCTGA GATGGGAATG CATACAGTCA GTGTCAAGTA CAAGGGCCAG 
CATGTACCTG GGAGCCCCTT CCAGTTCACT GTGGGGCCTC TGGGGGAAGG GGGTGCTCAC 
AAGGTCCGTG CTGGAGGCCC TGGCCTAGAG AGGGCTGAAG TTGGAGTGCC AGCGGAGTTC 
GGCATTTGGA CTAGGGAAGC TGGCGCTGGA GGCCTGGCCA TTGCTGTTGA AGGCCCCAGC 
AAGGCTGAGA TCTCTTTCGA GGACCGAAAG GATGGCTCCT GTGGTGTGGC CTACGTAGTT 
CAGGAGCCAG GTGACTATGA GGTCTCAGTC AAGTTCAACG AGGAGCACAT ACCTGATAGC 
CCCTTCGTGG TGCCTGTGGC TTCTCCGTCT GGTGACGCCC GCCGCCTTAC TGTTTCTAGT 
CTTCAGGAGT CAGGGTTAAA GGTCAACCAG CCAGCATCTT TTGCAGTCAG TCTGAATGGA 
GCCAAGGGGG CAATTGATGC CAAGGTGCAC AGCCCCTCAG GAGCTCTGGA GGAGTGCTAT 
GTCACAGAGA TTGACCAAGA TAAGTATGCT GTGCGTTTCA TCCCACGAGA GAATGGCATC 
TACTTGATTG ATGTCAAGTT CAATGGTACT CACATTCCTG GAAGTCCCTT CAAGATCCGA 
GTTGGGGAGC CTGGGCATGG AGGGGACCCA GGCTTAGTGT CCGCCTATGG AGCAGGCCTG 
GAAGGTGGTG TCACAGGGAG CCCAGCAGAG TTTATTGTGA ACACAAGCAA TGCAGGAGCT 
GGTGCCCTTT CGGTTACCAT TGATGGCCCC TCCAAGGTGA AGATGGATTG CCAGGAGTGC 
CCCGAGGGCT ATCGTGTCAC CTATACCCCC ATGGCACCTG GCAGCTACCT CATCTCCATC 
AAGTATGGTG GCCCCTATCA CATTGGTGGA AGTCCCTTTA AAGCCAAGGT CACAGGTCCT 
CGTCTTGTAA GCAACCACAG CCTCCATGAG ACATCATCTG TGTTTGTGGA CTCTTTGACT 
AAAGTTGCCA CGGTTCCCCA GCATGCAACC TCAGGCCCAG GTCCTGCTGA TGTCAGCAAG 
GTAGTAGCCA AAGGCCTGGG GCTAAGCAAG GCTTATGTAG GCCAGAAGAG CAACTTCACA 
GTAGATTGCA GCAAAGCAGG TAACAACATG CTGCTGGTGG GCGTGCATGG CCCAAGGACA 
CCCTGTGAAG AGATCCTGGT GAAACACATG GGCAGCCGCC TCTATAGTGT CTCTTACCTG 
CTCAAGGACA AAGGGGAGTA CACACTGGTG GTCAAGTGGG GTGATGAGCA TATCCCAGGC 
AGCCCATACC GCATTATGGT ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173922.1
CDS	2056..8022
Translation	MADIREAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAKHAGKAPLRVQVQDNEGCSVEATVKDNGNGTYSCSYVPRKPVKHTAMVSWGGVSIPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCTEAGQGDVSIGIKCAPGVVGPTEADIDFDIIRNDNDTFTVKYTPCGAGSYTIMVLFADQATPTSPIRVKVEPSHDASKVKAEGPGLNRTGVELGKPTHFTVNAKTAGKGKLDVQFSGLAKGDAVRDVDIIDHHDNTYTVKYIPVQQGPVGVNVTYGGDHIPKSPFSVGVSPSLDLSKIKVSGLGDKVDVGKDQEFTVKSKGAGGQGKVASKIVSPSGAAVPCKVEPGLGADNSVVRFVPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQGGNAGSPARFTIDTKGAGTGGLGLTVEGPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVAPCFDASKVKCSGPGLERATAGEVGQFQVDCSSAGSAELTIEICSEAGLPAEVYIQDHGDGTHTITYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTGGPHVKARVANPSGNLTDTYVQDCGDGTYKVEYTPYEEGVHSVDVTYDGSPVPSSPFQVPVTEGCDPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKMSCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDVVDNADGTQTVNYVPSREGSYSISVLYGEEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVPDVPGRYTILIKYGGDEIPFSPYRVRAVPTGDASKCTVTVSIGGHGLGAGIGPTIQIGEETVITVDTKAAGKGKVTCTVCTPDGSEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPTVQTPLRSQQLAPQYNYPQGSQQTWIPERPMVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPSITDNKDGTVTVRYSPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHITAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNDQHIPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANIGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGMHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEVGVPAEFGIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGIYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGSPAEFIVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKVATVPQHATSGPGPADVSKVVAKGLGLSKAYVGQKSNFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHMGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRIMVP

Target ORF information:

RefSeq Version	XM_017318433.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus filamin, alpha (Flna), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318433.2

ORF Insert Sequence:

ATGGCTGACA TCCGTGAGGC ACCCCAGGAT TTTCACCCAG ACAGGGTGAA GGCACGTGGG 
CCTGGATTGG AGAAGACTGG TGTGGCTGTC AACAAGCCAG CAGAGTTCAC AGTTGATGCC 
AAGCATGCTG GGAAGGCTCC TCTTCGAGTT CAAGTTCAGG ACAATGAGGG CTGCTCTGTG 
GAAGCGACAG TCAAGGACAA TGGCAATGGT ACTTACAGCT GTTCTTATGT GCCCAGAAAG 
CCAGTGAAGC ACACAGCCAT GGTTTCTTGG GGAGGTGTCA GCATCCCCAA CAGTCCTTTC 
CGGGTGAATG TGGGAGCTGG CAGCCATCCA AACAAAGTCA AGGTGTATGG TCCAGGAGTG 
GCCAAGACTG GGCTCAAGGC CCATGAACCT ACCTACTTTA CTGTGGATTG TACTGAAGCT 
GGCCAGGGAG ATGTCAGCAT TGGTATCAAG TGTGCCCCTG GAGTAGTGGG CCCCACTGAG 
GCTGATATTG ACTTTGATAT CATCCGCAAT GACAATGACA CCTTCACTGT AAAATACACA 
CCCTGTGGGG CTGGCAGCTA TACCATCATG GTCCTTTTTG CTGACCAGGC CACACCCACC 
AGCCCCATCA GAGTCAAAGT GGAGCCTTCT CATGATGCCA GCAAGGTGAA GGCTGAGGGT 
CCTGGCCTAA ATCGCACTGG TGTTGAACTT GGCAAACCCA CCCATTTCAC AGTCAATGCT 
AAAACTGCTG GGAAAGGCAA GCTGGATGTC CAATTCTCAG GACTGGCTAA GGGAGATGCA 
GTACGGGATG TGGACATCAT TGACCACCAT GATAATACCT ACACAGTCAA GTACATTCCT 
GTGCAGCAGG GCCCAGTAGG TGTCAATGTC ACTTATGGAG GAGATCACAT CCCCAAGAGT 
CCATTTTCAG TGGGAGTATC TCCAAGCCTG GATCTCAGCA AAATCAAGGT GTCTGGCCTT 
GGTGACAAAG TGGACGTTGG CAAAGATCAA GAGTTCACAG TAAAGTCAAA GGGTGCAGGT 
GGTCAAGGCA AAGTAGCATC CAAGATTGTG AGTCCCTCAG GTGCAGCGGT ACCCTGCAAG 
GTAGAGCCAG GCCTGGGAGC TGACAACAGC GTGGTACGTT TTGTGCCCCG TGAAGAGGGG 
CCCTATGAGG TGGAAGTGAC CTATGATGGT GTGCCTGTAC CTGGCAGTCC CTTTCCACTA 
GAAGCTGTGG CCCCCACCAA ACCCAGCAAG GTGAAGGCGT TTGGACCAGG GCTACAGGGG 
GGCAATGCAG GCTCCCCTGC CCGCTTCACC ATTGATACAA AGGGTGCTGG CACTGGTGGC 
CTGGGCCTGA CAGTGGAAGG CCCCTGTGAA GCACAGCTTG AGTGCCTAGA CAACGGGGAT 
GGTACATGCT CTGTGTCTTA TGTACCCACT GAGCCTGGGG ACTACAACAT CAACATCCTT 
TTTGCTGACA CCCACATTCC TGGATCCCCA TTCAAGGCCC ATGTGGCTCC TTGTTTTGAT 
GCATCCAAGG TGAAGTGCTC AGGCCCTGGG CTGGAGCGGG CTACTGCTGG TGAGGTAGGG 
CAGTTCCAAG TGGACTGTTC AAGTGCTGGC AGTGCTGAGT TGACGATTGA GATCTGCTCT 
GAGGCAGGAC TGCCAGCTGA AGTATACATT CAAGACCATG GTGATGGCAC ACACACCATT 
ACCTATATTC CTCTCTGTCC TGGGGCTTAC ACTGTTACCA TCAAGTATGG CGGCCAGCCT 
GTGCCCAACT TCCCCAGCAA GCTACAGGTG GAACCTGCTG TAGATACCTC AGGTGTACAG 
TGCTATGGGC CTGGGATTGA AGGTCAAGGT GTCTTCCGAG AGGCAACCAC TGAGTTCAGT 
GTGGATGCCC GGGCTCTTAC ACAGACTGGA GGGCCACATG TCAAGGCTCG AGTGGCCAAC 
CCCTCAGGCA ATCTGACAGA TACCTATGTG CAAGACTGTG GGGATGGCAC ATACAAAGTG 
GAATACACTC CATATGAGGA AGGAGTACAC TCTGTGGATG TGACTTATGA TGGCAGCCCT 
GTGCCCAGCA GCCCCTTCCA GGTGCCTGTA ACAGAGGGCT GTGACCCCTC CCGGGTGCGT 
GTCCATGGAC CAGGCATCCA AAGTGGTACC ACCAACAAAC CCAACAAGTT CACAGTAGAA 
ACCAGGGGAG CTGGCACAGG TGGCCTGGGC TTGGCTGTTG AGGGTCCCTC AGAGGCCAAG 
ATGTCTTGTA TGGATAATAA AGATGGCAGC TGCTCAGTAG AATACATCCC CTATGAAGCT 
GGAACCTATA GCCTTAATGT CACTTATGGT GGTCACCAAG TGCCAGGTAG TCCCTTCAAG 
GTCCCTGTAC ATGATGTGAC AGATGCATCT AAAGTCAAGT GTTCTGGACC TGGCCTAAGC 
CCAGGCATGG TCCGTGCCAA CCTCCCTCAG TCCTTTCAGG TGGACACAAG CAAAGCTGGA 
GTTGCCCCAC TGCAGGTCAA AGTGCAGGGG CCCAAAGGCC TGGTGGAGCC AGTGGATGTA 
GTGGACAATG CTGATGGTAC TCAGACTGTC AACTATGTGC CCAGCCGAGA AGGGTCCTAT 
AGCATTTCTG TGCTGTATGG TGAAGAAGAA GTGCCACGGA GCCCCTTCAA GGTCAAGGTG 
CTGCCTACAC ATGATGCCAG TAAGGTGAAG GCCAGTGGAC CTGGACTCAA CACCACTGGT 
GTACCTGCTA GCCTGCCTGT GGAGTTCACC ATTGATGCCA AGGATGCTGG GGAGGGTCTG 
TTGGCTGTCC AGATTACGGA TCCTGAAGGC AAGCCCAAGA AGACACACAT TCAAGATAAT 
CATGATGGCA CATACACGGT GGCTTATGTG CCAGATGTGC CAGGCCGGTA CACAATCCTC 
ATCAAGTATG GTGGTGATGA GATTCCCTTT TCCCCGTACC GTGTCCGGGC TGTGCCCACT 
GGGGATGCCA GCAAGTGCAC AGTCACAGTG TCAATCGGAG GTCACGGGCT AGGTGCTGGC 
ATTGGCCCCA CCATCCAGAT TGGGGAGGAG ACGGTGATTA CTGTGGACAC AAAAGCAGCA 
GGCAAAGGCA AAGTGACTTG TACTGTGTGC ACACCTGATG GCTCAGAGGT AGACGTGGAC 
GTGGTGGAGA ATGAGGATGG CACCTTTGAC ATCTTCTACA CAGCTCCCCA ACCGGGCAAA 
TATGTCATCT GTGTGCGCTT CGGTGGCGAG CATGTGCCCA ACAGCCCCTT CCAAGTTACA 
GCTTTGGCTG GGGACCAACC AACAGTGCAG ACCCCATTAA GGTCTCAGCA GCTGGCTCCA 
CAGTATAACT ATCCTCAGGG TAGCCAGCAA ACCTGGATTC CAGAGAGGCC CATGGTGGGC 
GTTAATGGGC TGGATGTGAC CAGCCTGAGG CCCTTTGATC TTGTCATCCC CTTCACTATC 
AAGAAGGGGG AGATCACTGG GGAAGTTCGA ATGCCCTCAG GCAAGGTGGC CCAGCCTTCC 
ATTACTGATA ACAAAGATGG CACTGTTACT GTACGTTACT CACCCAGTGA AGCTGGCCTG 
CATGAAATGG ACATTCGCTA TGACAATATG CATATCCCAG GAAGCCCTCT GCAGTTCTAT 
GTTGATTATG TCAACTGTGG CCACATCACT GCTTATGGTC CTGGCCTTAC CCATGGAGTG 
GTCAACAAAC CTGCCACCTT CACTGTCAAC ACCAAGGATG CAGGAGAGGG GGGCTTGTCT 
CTGGCCATTG AGGGTCCATC TAAAGCAGAA ATCAGTTGCA CTGACAACCA GGATGGAACA 
TGCAGTGTCT CTTACCTGCC TGTACTGCCT GGTGACTATA GCATCCTAGT TAAGTACAAT 
GATCAACACA TCCCAGGCAG TCCCTTTACT GCCAGAGTAA CAGGTGACGA TTCCATGCGT 
ATGTCCCACC TAAAGGTGGG TTCTGCTGCT GATATCCCCA TCAATATCTC AGAAACAGAC 
CTTAGCCTAC TCACAGCCAC TGTGGTGCCA CCTTCGGGTC GAGAGGAACC CTGTCTGCTG 
AAACGTTTGC GAAATGGCCA CGTGGGGATT TCCTTCGTGC CCAAGGAGAC AGGGGAGCAC 
CTGGTACATG TGAAGAAGAA TGGCCAGCAT GTGGCAAGCA GTCCCATCCC AGTAGTGATC 
AGCCAGTCGG AGATAGGTGA TGCCAGCCGT GTGAGGGTCT CTGGTCAAGG TCTTCATGAA 
GGTCATACCT TTGAGCCTGC AGAGTTTATT ATTGACACCA GAGATGCAGG CTACGGTGGG 
CTTAGTCTGT CCATTGAGGG CCCTAGCAAA GTAGACATCA ACACAGAGGA TCTGGAGGAT 
GGCACATGCA GGGTCACCTA CTGTCCCACA GAGCCTGGAA ACTACATTAT AAACATCAAA 
TTTGCTGACC AGCATGTGCC TGGCAGTCCC TTTTCTGTGA AGGTGACAGG TGAGGGCCGG 
GTGAAAGAGA GTATCACACG CAGGCGACGT GCCCCTTCTG TGGCCAATAT TGGCAGTCAT 
TGTGACCTCA GCCTGAAGAT TCCTGAAATT AGCATCCAAG ATATGACAGC CCAGGTGACC 
AGCCCATCAG GCAAGACCCA TGAGGCAGAG ATCGTAGAAG GAGAGAACCA TACTTACTGT 
ATCCGATTTG TGCCTGCTGA GATGGGAATG CATACAGTCA GTGTCAAGTA CAAGGGCCAG 
CATGTACCTG GGAGCCCCTT CCAGTTCACT GTGGGGCCTC TGGGGGAAGG GGGTGCTCAC 
AAGGTCCGTG CTGGAGGCCC TGGCCTAGAG AGGGCTGAAG TTGGAGTGCC AGCGGAGTTC 
GGCATTTGGA CTAGGGAAGC TGGCGCTGGA GGCCTGGCCA TTGCTGTTGA AGGCCCCAGC 
AAGGCTGAGA TCTCTTTCGA GGACCGAAAG GATGGCTCCT GTGGTGTGGC CTACGTAGTT 
CAGGAGCCAG GTGACTATGA GGTCTCAGTC AAGTTCAACG AGGAGCACAT ACCTGATAGC 
CCCTTCGTGG TGCCTGTGGC TTCTCCGTCT GGTGACGCCC GCCGCCTTAC TGTTTCTAGT 
CTTCAGGAGT CAGGGTTAAA GGTCAACCAG CCAGCATCTT TTGCAGTCAG TCTGAATGGA 
GCCAAGGGGG CAATTGATGC CAAGGTGCAC AGCCCCTCAG GAGCTCTGGA GGAGTGCTAT 
GTCACAGAGA TTGACCAAGA TAAGTATGCT GTGCGTTTCA TCCCACGAGA GAATGGCATC 
TACTTGATTG ATGTCAAGTT CAATGGTACT CACATTCCTG GAAGTCCCTT CAAGATCCGA 
GTTGGGGAGC CTGGGCATGG AGGGGACCCA GGCTTAGTGT CCGCCTATGG AGCAGGCCTG 
GAAGGTGGTG TCACAGGGAG CCCAGCAGAG TTTATTGTGA ACACAAGCAA TGCAGGAGCT 
GGTGCCCTTT CGGTTACCAT TGATGGCCCC TCCAAGGTGA AGATGGATTG CCAGGAGTGC 
CCCGAGGGCT ATCGTGTCAC CTATACCCCC ATGGCACCTG GCAGCTACCT CATCTCCATC 
AAGTATGGTG GCCCCTATCA CATTGGTGGA AGTCCCTTTA AAGCCAAGGT CACAGGTCCT 
CGTCTTGTAA GCAACCACAG CCTCCATGAG ACATCATCTG TGTTTGTGGA CTCTTTGACT 
AAAGTTGCCA CGGTTCCCCA GCATGCAACC TCAGGCCCAG GTCCTGCTGA TGTCAGCAAG 
GTAGTAGCCA AAGGCCTGGG GCTAAGCAAG GCTTATGTAG GCCAGAAGAG CAACTTCACA 
GTAGATTGCA GCAAAGCAGG TAACAACATG CTGCTGGTGG GCGTGCATGG CCCAAGGACA 
CCCTGTGAAG AGATCCTGGT GAAACACATG GGCAGCCGCC TCTATAGTGT CTCTTACCTG 
CTCAAGGACA AAGGGGAGTA CACACTGGTG GTCAAGTGGG GTGATGAGCA TATCCCAGGC 
AGCCCATACC GCATTATGGT ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Flna ( NM_010227.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034357.2 Mus musculus filamin, alpha (Flna), transcript variant 1, mRNA.

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CloneID	OMu20363
Clone ID Related Accession (Same CDS sequence)	XM_011247549.2 , XM_011247549.3 , NM_010227.3
Accession Version	NM_010227.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Mus musculus(house mouse)
Product	filamin-A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL807376.4. On or before Mar 12, 2014 this sequence version replaced XM_006527912.1, NM_010227.2. Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC151024.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164133 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTAGCT CTCACTCCCG CTGTGGCCAG AGTGCGGCGG TTGCGTCTCC GGGAGGCAGT 
ATCGATTCAC GGGACGCGGA GATGCCGGCT ACCGAAAAAG ACCTAGCAGA AGATGCACCG 
TGGAAGAAAA TTCAGCAGAA CACATTCACC CGCTGGTGCA ATGAGCACCT TAAGTGCGTA 
AGCAAGCGCA TCGCCAATCT GCAGACGGAC CTGAGCGATG GGTTGCGGCT CATCGCGCTG 
CTCGAGGTAC TCAGCCAGAA GAAAATGCAC CGCAAGCACA ACCAAAGACC CACTTTCCGC 
CAGATGCAGC TCGAAAATGT GTCGGTGGCG CTTGAATTCC TGGACCGTGA GAGCATCAAG 
CTCGTGTCCA TAGACAGCAA GGCTATTGTG GATGGAAATC TGAAGCTGAT CTTAGGCCTC 
ATCTGGACCC TGATCCTGCA CTATTCCATC TCAATGCCCA TGTGGGATGA GGAAGAGGAT 
GAGGAGGCCA AGAAGCAAAC ACCCAAGCAG AGGCTTCTAG GCTGGATTCA GAACAAGCTA 
CCACAGCTTC CCATTACCAA CTTCAGTCGA GACTGGCAGA GTGGCCGGGC CCTGGGTGCT 
CTTGTTGATA GCTGTGCCCC AGGCCTATGT CCTGACTGGG ACTCCTGGGA TGCTAGTAAG 
CCTGTGAACA ATGCACGGGA AGCCATGCAG CAGGCTGATG ACTGGCTAGG CATTCCTCAG 
GTGATTACCC CAGAAGAAAT TGTGGACCCC AATGTAGATG AGCATTCTGT TATGACCTAC 
CTGTCTCAGT TTCCCAAGGC CAAGCTGAAG CCAGGGGCTC CTCTTCGGCC CAAACTGAAC 
CCGAAGAAAG CCCGAGCCTA TGGGCCAGGC ATCGAGCCTA CAGGCAATAT GGTGAAGAAG 
AGAGCAGAAT TCACTGTGGA GACCCGAAGT GCTGGACAGG GAGAAGTGCT TGTATATGTG 
GAGGACCCAG CTGGACACCA GGAAGAGGCA AAAGTGACTG CCAATAATGA CAAGAACCGT 
ACTTTCTCTG TCTGGTATGT CCCTGAAGTG ACAGGGACTC ATAAGGTGAC TGTGCTCTTT 
GCTGGCCAAC ATATTGCCAA GAGCCCCTTT GAGGTGTATG TGGACAAGTC ACAGGGTGAT 
GCCAGCAAAG TGACTGCCCA GGGCCCTGGT CTGGAGCCCA GTGGCAATAT TGCCAACAAG 
ACTACCTACT TTGAGATCTT CACTGCAGGA GCTGGCATGG GTGAGGTGGA AGTTGTCATC 
CAGGACCCTA CAGGACAGAA AGGCACAGTG GAACCTCAGC TGGAGGCCAG GGGTGACAGC 
ACCTATCGCT GTAGCTATCA GCCCACCATG GAGGGTGTCC ATACAGTACA TGTCACCTTC 
GCCGGTGTTC CCATCCCTCG TAGCCCCTAC ACTGTCACTG TTGGCCAAGC CTGTAACCCA 
GCTGCCTGCC GGGCTATTGG TAGAGGCCTT CAGCCCAAGG GTGTTCGAGT GAAGGAAACA 
GCCGACTTCA AGGTGTACAC AAAGGGCGCT GGCAGTGGGG AGCTAAAGGT CACTGTAAAG 
GGTCCCAAGG GTGAGGAGCG TGTAAAGCAG AAGGACTTAG GGGATGGTGT GTATGGCTTT 
GAATATTACC CTACAATCCC TGGCACATAC ACTGTCACCA TCACATGGGG TGGCCAGAAC 
ATTGGTCGAA GTCCGTTTGA GGTGAAGGTA GGCACTGAGT GTGGCAATCA GAAAGTTCGG 
GCATGGGGTC CTGGCCTGGA AGGAGGCATT GTTGGCAAGT CAGCAGACTT CGTAGTAGAG 
GCCATTGGTG ATGATGTGGG CACCTTGGGT TTCTCTGTGG AAGGTCCATC ACAGGCAAAG 
ATTGAATGTG ACGACAAGGG TGATGGCTCC TGTGATGTGC GCTATTGGCC CCAGGAGGCT 
GGCGAGTATG CTGTTCATGT GCTGTGTAAC AGTGAGGATA TCCGTCTCAG TCCTTTCATG 
GCTGACATCC GTGAGGCACC CCAGGATTTT CACCCAGACA GGGTGAAGGC ACGTGGGCCT 
GGATTGGAGA AGACTGGTGT GGCTGTCAAC AAGCCAGCAG AGTTCACAGT TGATGCCAAG 
CATGCTGGGA AGGCTCCTCT TCGAGTTCAA GTTCAGGACA ATGAGGGCTG CTCTGTGGAA 
GCGACAGTCA AGGACAATGG CAATGGTACT TACAGCTGTT CTTATGTGCC CAGAAAGCCA 
GTGAAGCACA CAGCCATGGT TTCTTGGGGA GGTGTCAGCA TCCCCAACAG TCCTTTCCGG 
GTGAATGTGG GAGCTGGCAG CCATCCAAAC AAAGTCAAGG TGTATGGTCC AGGAGTGGCC 
AAGACTGGGC TCAAGGCCCA TGAACCTACC TACTTTACTG TGGATTGTAC TGAAGCTGGC 
CAGGGAGATG TCAGCATTGG TATCAAGTGT GCCCCTGGAG TAGTGGGCCC CACTGAGGCT 
GATATTGACT TTGATATCAT CCGCAATGAC AATGACACCT TCACTGTAAA ATACACACCC 
TGTGGGGCTG GCAGCTATAC CATCATGGTC CTTTTTGCTG ACCAGGCCAC ACCCACCAGC 
CCCATCAGAG TCAAAGTGGA GCCTTCTCAT GATGCCAGCA AGGTGAAGGC TGAGGGTCCT 
GGCCTAAATC GCACTGGTGT TGAACTTGGC AAACCCACCC ATTTCACAGT CAATGCTAAA 
ACTGCTGGGA AAGGCAAGCT GGATGTCCAA TTCTCAGGAC TGGCTAAGGG AGATGCAGTA 
CGGGATGTGG ACATCATTGA CCACCATGAT AATACCTACA CAGTCAAGTA CATTCCTGTG 
CAGCAGGGCC CAGTAGGTGT CAATGTCACT TATGGAGGAG ATCACATCCC CAAGAGTCCA 
TTTTCAGTGG GAGTATCTCC AAGCCTGGAT CTCAGCAAAA TCAAGGTGTC TGGCCTTGGT 
GACAAAGTGG ACGTTGGCAA AGATCAAGAG TTCACAGTAA AGTCAAAGGG TGCAGGTGGT 
CAAGGCAAAG TAGCATCCAA GATTGTGAGT CCCTCAGGTG CAGCGGTACC CTGCAAGGTA 
GAGCCAGGCC TGGGAGCTGA CAACAGCGTG GTACGTTTTG TGCCCCGTGA AGAGGGGCCC 
TATGAGGTGG AAGTGACCTA TGATGGTGTG CCTGTACCTG GCAGTCCCTT TCCACTAGAA 
GCTGTGGCCC CCACCAAACC CAGCAAGGTG AAGGCGTTTG GACCAGGGCT ACAGGGGGGC 
AATGCAGGCT CCCCTGCCCG CTTCACCATT GATACAAAGG GTGCTGGCAC TGGTGGCCTG 
GGCCTGACAG TGGAAGGCCC CTGTGAAGCA CAGCTTGAGT GCCTAGACAA CGGGGATGGT 
ACATGCTCTG TGTCTTATGT ACCCACTGAG CCTGGGGACT ACAACATCAA CATCCTTTTT 
GCTGACACCC ACATTCCTGG ATCCCCATTC AAGGCCCATG TGGCTCCTTG TTTTGATGCA 
TCCAAGGTGA AGTGCTCAGG CCCTGGGCTG GAGCGGGCTA CTGCTGGTGA GGTAGGGCAG 
TTCCAAGTGG ACTGTTCAAG TGCTGGCAGT GCTGAGTTGA CGATTGAGAT CTGCTCTGAG 
GCAGGACTGC CAGCTGAAGT ATACATTCAA GACCATGGTG ATGGCACACA CACCATTACC 
TATATTCCTC TCTGTCCTGG GGCTTACACT GTTACCATCA AGTATGGCGG CCAGCCTGTG 
CCCAACTTCC CCAGCAAGCT ACAGGTGGAA CCTGCTGTAG ATACCTCAGG TGTACAGTGC 
TATGGGCCTG GGATTGAAGG TCAAGGTGTC TTCCGAGAGG CAACCACTGA GTTCAGTGTG 
GATGCCCGGG CTCTTACACA GACTGGAGGG CCACATGTCA AGGCTCGAGT GGCCAACCCC 
TCAGGCAATC TGACAGATAC CTATGTGCAA GACTGTGGGG ATGGCACATA CAAAGTGGAA 
TACACTCCAT ATGAGGAAGG AGTACACTCT GTGGATGTGA CTTATGATGG CAGCCCTGTG 
CCCAGCAGCC CCTTCCAGGT GCCTGTAACA GAGGGCTGTG ACCCCTCCCG GGTGCGTGTC 
CATGGACCAG GCATCCAAAG TGGTACCACC AACAAACCCA ACAAGTTCAC AGTAGAAACC 
AGGGGAGCTG GCACAGGTGG CCTGGGCTTG GCTGTTGAGG GTCCCTCAGA GGCCAAGATG 
TCTTGTATGG ATAATAAAGA TGGCAGCTGC TCAGTAGAAT ACATCCCCTA TGAAGCTGGA 
ACCTATAGCC TTAATGTCAC TTATGGTGGT CACCAAGTGC CAGGTAGTCC CTTCAAGGTC 
CCTGTACATG ATGTGACAGA TGCATCTAAA GTCAAGTGTT CTGGACCTGG CCTAAGCCCA 
GGCATGGTCC GTGCCAACCT CCCTCAGTCC TTTCAGGTGG ACACAAGCAA AGCTGGAGTT 
GCCCCACTGC AGGTCAAAGT GCAGGGGCCC AAAGGCCTGG TGGAGCCAGT GGATGTAGTG 
GACAATGCTG ATGGTACTCA GACTGTCAAC TATGTGCCCA GCCGAGAAGG GTCCTATAGC 
ATTTCTGTGC TGTATGGTGA AGAAGAAGTG CCACGGAGCC CCTTCAAGGT CAAGGTGCTG 
CCTACACATG ATGCCAGTAA GGTGAAGGCC AGTGGACCTG GACTCAACAC CACTGGTGTA 
CCTGCTAGCC TGCCTGTGGA GTTCACCATT GATGCCAAGG ATGCTGGGGA GGGTCTGTTG 
GCTGTCCAGA TTACGGATCC TGAAGGCAAG CCCAAGAAGA CACACATTCA AGATAATCAT 
GATGGCACAT ACACGGTGGC TTATGTGCCA GATGTGCCAG GCCGGTACAC AATCCTCATC 
AAGTATGGTG GTGATGAGAT TCCCTTTTCC CCGTACCGTG TCCGGGCTGT GCCCACTGGG 
GATGCCAGCA AGTGCACAGT CACAGGTGCT GGCATTGGCC CCACCATCCA GATTGGGGAG 
GAGACGGTGA TTACTGTGGA CACAAAAGCA GCAGGCAAAG GCAAAGTGAC TTGTACTGTG 
TGCACACCTG ATGGCTCAGA GGTAGACGTG GACGTGGTGG AGAATGAGGA TGGCACCTTT 
GACATCTTCT ACACAGCTCC CCAACCGGGC AAATATGTCA TCTGTGTGCG CTTCGGTGGC 
GAGCATGTGC CCAACAGCCC CTTCCAAGTT ACAGCTTTGG CTGGGGACCA ACCAACAGTG 
CAGACCCCAT TAAGGTCTCA GCAGCTGGCT CCACAGTATA ACTATCCTCA GGGTAGCCAG 
CAAACCTGGA TTCCAGAGAG GCCCATGGTG GGCGTTAATG GGCTGGATGT GACCAGCCTG 
AGGCCCTTTG ATCTTGTCAT CCCCTTCACT ATCAAGAAGG GGGAGATCAC TGGGGAAGTT 
CGAATGCCCT CAGGCAAGGT GGCCCAGCCT TCCATTACTG ATAACAAAGA TGGCACTGTT 
ACTGTACGTT ACTCACCCAG TGAAGCTGGC CTGCATGAAA TGGACATTCG CTATGACAAT 
ATGCATATCC CAGGAAGCCC TCTGCAGTTC TATGTTGATT ATGTCAACTG TGGCCACATC 
ACTGCTTATG GTCCTGGCCT TACCCATGGA GTGGTCAACA AACCTGCCAC CTTCACTGTC 
AACACCAAGG ATGCAGGAGA GGGGGGCTTG TCTCTGGCCA TTGAGGGTCC ATCTAAAGCA 
GAAATCAGTT GCACTGACAA CCAGGATGGA ACATGCAGTG TCTCTTACCT GCCTGTACTG 
CCTGGTGACT ATAGCATCCT AGTTAAGTAC AATGATCAAC ACATCCCAGG CAGTCCCTTT 
ACTGCCAGAG TAACAGGTGA CGATTCCATG CGTATGTCCC ACCTAAAGGT GGGTTCTGCT 
GCTGATATCC CCATCAATAT CTCAGAAACA GACCTTAGCC TACTCACAGC CACTGTGGTG 
CCACCTTCGG GTCGAGAGGA ACCCTGTCTG CTGAAACGTT TGCGAAATGG CCACGTGGGG 
ATTTCCTTCG TGCCCAAGGA GACAGGGGAG CACCTGGTAC ATGTGAAGAA GAATGGCCAG 
CATGTGGCAA GCAGTCCCAT CCCAGTAGTG ATCAGCCAGT CGGAGATAGG TGATGCCAGC 
CGTGTGAGGG TCTCTGGTCA AGGTCTTCAT GAAGGTCATA CCTTTGAGCC TGCAGAGTTT 
ATTATTGACA CCAGAGATGC AGGCTACGGT GGGCTTAGTC TGTCCATTGA GGGCCCTAGC 
AAAGTAGACA TCAACACAGA GGATCTGGAG GATGGCACAT GCAGGGTCAC CTACTGTCCC 
ACAGAGCCTG GAAACTACAT TATAAACATC AAATTTGCTG ACCAGCATGT GCCTGGCAGT 
CCCTTTTCTG TGAAGGTGAC AGGTGAGGGC CGGGTGAAAG AGAGTATCAC ACGCAGGCGA 
CGTGCCCCTT CTGTGGCCAA TATTGGCAGT CATTGTGACC TCAGCCTGAA GATTCCTGAA 
ATTAGCATCC AAGATATGAC AGCCCAGGTG ACCAGCCCAT CAGGCAAGAC CCATGAGGCA 
GAGATCGTAG AAGGAGAGAA CCATACTTAC TGTATCCGAT TTGTGCCTGC TGAGATGGGA 
ATGCATACAG TCAGTGTCAA GTACAAGGGC CAGCATGTAC CTGGGAGCCC CTTCCAGTTC 
ACTGTGGGGC CTCTGGGGGA AGGGGGTGCT CACAAGGTCC GTGCTGGAGG CCCTGGCCTA 
GAGAGGGCTG AAGTTGGAGT GCCAGCGGAG TTCGGCATTT GGACTAGGGA AGCTGGCGCT 
GGAGGCCTGG CCATTGCTGT TGAAGGCCCC AGCAAGGCTG AGATCTCTTT CGAGGACCGA 
AAGGATGGCT CCTGTGGTGT GGCCTACGTA GTTCAGGAGC CAGGTGACTA TGAGGTCTCA 
GTCAAGTTCA ACGAGGAGCA CATACCTGAT AGCCCCTTCG TGGTGCCTGT GGCTTCTCCG 
TCTGGTGACG CCCGCCGCCT TACTGTTTCT AGTCTTCAGG AGTCAGGGTT AAAGGTCAAC 
CAGCCAGCAT CTTTTGCAGT CAGTCTGAAT GGAGCCAAGG GGGCAATTGA TGCCAAGGTG 
CACAGCCCCT CAGGAGCTCT GGAGGAGTGC TATGTCACAG AGATTGACCA AGATAAGTAT 
GCTGTGCGTT TCATCCCACG AGAGAATGGC ATCTACTTGA TTGATGTCAA GTTCAATGGT 
ACTCACATTC CTGGAAGTCC CTTCAAGATC CGAGTTGGGG AGCCTGGGCA TGGAGGGGAC 
CCAGGCTTAG TGTCCGCCTA TGGAGCAGGC CTGGAAGGTG GTGTCACAGG GAGCCCAGCA 
GAGTTTATTG TGAACACAAG CAATGCAGGA GCTGGTGCCC TTTCGGTTAC CATTGATGGC 
CCCTCCAAGG TGAAGATGGA TTGCCAGGAG TGCCCCGAGG GCTATCGTGT CACCTATACC 
CCCATGGCAC CTGGCAGCTA CCTCATCTCC ATCAAGTATG GTGGCCCCTA TCACATTGGT 
GGAAGTCCCT TTAAAGCCAA GGTCACAGGT CCTCGTCTTG TAAGCAACCA CAGCCTCCAT 
GAGACATCAT CTGTGTTTGT GGACTCTTTG ACTAAAGTTG CCACGGTTCC CCAGCATGCA 
ACCTCAGGCC CAGGTCCTGC TGATGTCAGC AAGGTAGTAG CCAAAGGCCT GGGGCTAAGC 
AAGGCTTATG TAGGCCAGAA GAGCAACTTC ACAGTAGATT GCAGCAAAGC AGGTAACAAC 
ATGCTGCTGG TGGGCGTGCA TGGCCCAAGG ACACCCTGTG AAGAGATCCT GGTGAAACAC 
ATGGGCAGCC GCCTCTATAG TGTCTCTTAC CTGCTCAAGG ACAAAGGGGA GTACACACTG 
GTGGTCAAGT GGGGTGATGA GCATATCCCA GGCAGCCCAT ACCGCATTAT GGTACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034357.2
CDS	99..8018
Translation	MSSSHSRCGQSAAVASPGGSIDSRDAEMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVSKRIANLQTDLSDGLRLIALLEVLSQKKMHRKHNQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEDEEAKKQTPKQRLLGWIQNKLPQLPITNFSRDWQSGRALGALVDSCAPGLCPDWDSWDASKPVNNAREAMQQADDWLGIPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNMVKKRAEFTVETRSAGQGEVLVYVEDPAGHQEEAKVTANNDKNRTFSVWYVPEVTGTHKVTVLFAGQHIAKSPFEVYVDKSQGDASKVTAQGPGLEPSGNIANKTTYFEIFTAGAGMGEVEVVIQDPTGQKGTVEPQLEARGDSTYRCSYQPTMEGVHTVHVTFAGVPIPRSPYTVTVGQACNPAACRAIGRGLQPKGVRVKETADFKVYTKGAGSGELKVTVKGPKGEERVKQKDLGDGVYGFEYYPTIPGTYTVTITWGGQNIGRSPFEVKVGTECGNQKVRAWGPGLEGGIVGKSADFVVEAIGDDVGTLGFSVEGPSQAKIECDDKGDGSCDVRYWPQEAGEYAVHVLCNSEDIRLSPFMADIREAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAKHAGKAPLRVQVQDNEGCSVEATVKDNGNGTYSCSYVPRKPVKHTAMVSWGGVSIPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCTEAGQGDVSIGIKCAPGVVGPTEADIDFDIIRNDNDTFTVKYTPCGAGSYTIMVLFADQATPTSPIRVKVEPSHDASKVKAEGPGLNRTGVELGKPTHFTVNAKTAGKGKLDVQFSGLAKGDAVRDVDIIDHHDNTYTVKYIPVQQGPVGVNVTYGGDHIPKSPFSVGVSPSLDLSKIKVSGLGDKVDVGKDQEFTVKSKGAGGQGKVASKIVSPSGAAVPCKVEPGLGADNSVVRFVPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQGGNAGSPARFTIDTKGAGTGGLGLTVEGPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVAPCFDASKVKCSGPGLERATAGEVGQFQVDCSSAGSAELTIEICSEAGLPAEVYIQDHGDGTHTITYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTGGPHVKARVANPSGNLTDTYVQDCGDGTYKVEYTPYEEGVHSVDVTYDGSPVPSSPFQVPVTEGCDPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKMSCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDVVDNADGTQTVNYVPSREGSYSISVLYGEEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVPDVPGRYTILIKYGGDEIPFSPYRVRAVPTGDASKCTVTGAGIGPTIQIGEETVITVDTKAAGKGKVTCTVCTPDGSEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPTVQTPLRSQQLAPQYNYPQGSQQTWIPERPMVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPSITDNKDGTVTVRYSPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHITAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNDQHIPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANIGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGMHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEVGVPAEFGIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGIYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGSPAEFIVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKVATVPQHATSGPGPADVSKVVAKGLGLSKAYVGQKSNFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHMGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRIMVP

Target ORF information:

RefSeq Version	NM_010227.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus filamin, alpha (Flna), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010227.3

ORF Insert Sequence:

ATGAGTAGCT CTCACTCCCG CTGTGGCCAG AGTGCGGCGG TTGCGTCTCC GGGAGGCAGT 
ATCGATTCAC GGGACGCGGA GATGCCGGCT ACCGAAAAAG ACCTAGCAGA AGATGCACCG 
TGGAAGAAAA TTCAGCAGAA CACATTCACC CGCTGGTGCA ATGAGCACCT TAAGTGCGTA 
AGCAAGCGCA TCGCCAATCT GCAGACGGAC CTGAGCGATG GGTTGCGGCT CATCGCGCTG 
CTCGAGGTAC TCAGCCAGAA GAAAATGCAC CGCAAGCACA ACCAAAGACC CACTTTCCGC 
CAGATGCAGC TCGAAAATGT GTCGGTGGCG CTTGAATTCC TGGACCGTGA GAGCATCAAG 
CTCGTGTCCA TAGACAGCAA GGCTATTGTG GATGGAAATC TGAAGCTGAT CTTAGGCCTC 
ATCTGGACCC TGATCCTGCA CTATTCCATC TCAATGCCCA TGTGGGATGA GGAAGAGGAT 
GAGGAGGCCA AGAAGCAAAC ACCCAAGCAG AGGCTTCTAG GCTGGATTCA GAACAAGCTA 
CCACAGCTTC CCATTACCAA CTTCAGTCGA GACTGGCAGA GTGGCCGGGC CCTGGGTGCT 
CTTGTTGATA GCTGTGCCCC AGGCCTATGT CCTGACTGGG ACTCCTGGGA TGCTAGTAAG 
CCTGTGAACA ATGCACGGGA AGCCATGCAG CAGGCTGATG ACTGGCTAGG CATTCCTCAG 
GTGATTACCC CAGAAGAAAT TGTGGACCCC AATGTAGATG AGCATTCTGT TATGACCTAC 
CTGTCTCAGT TTCCCAAGGC CAAGCTGAAG CCAGGGGCTC CTCTTCGGCC CAAACTGAAC 
CCGAAGAAAG CCCGAGCCTA TGGGCCAGGC ATCGAGCCTA CAGGCAATAT GGTGAAGAAG 
AGAGCAGAAT TCACTGTGGA GACCCGAAGT GCTGGACAGG GAGAAGTGCT TGTATATGTG 
GAGGACCCAG CTGGACACCA GGAAGAGGCA AAAGTGACTG CCAATAATGA CAAGAACCGT 
ACTTTCTCTG TCTGGTATGT CCCTGAAGTG ACAGGGACTC ATAAGGTGAC TGTGCTCTTT 
GCTGGCCAAC ATATTGCCAA GAGCCCCTTT GAGGTGTATG TGGACAAGTC ACAGGGTGAT 
GCCAGCAAAG TGACTGCCCA GGGCCCTGGT CTGGAGCCCA GTGGCAATAT TGCCAACAAG 
ACTACCTACT TTGAGATCTT CACTGCAGGA GCTGGCATGG GTGAGGTGGA AGTTGTCATC 
CAGGACCCTA CAGGACAGAA AGGCACAGTG GAACCTCAGC TGGAGGCCAG GGGTGACAGC 
ACCTATCGCT GTAGCTATCA GCCCACCATG GAGGGTGTCC ATACAGTACA TGTCACCTTC 
GCCGGTGTTC CCATCCCTCG TAGCCCCTAC ACTGTCACTG TTGGCCAAGC CTGTAACCCA 
GCTGCCTGCC GGGCTATTGG TAGAGGCCTT CAGCCCAAGG GTGTTCGAGT GAAGGAAACA 
GCCGACTTCA AGGTGTACAC AAAGGGCGCT GGCAGTGGGG AGCTAAAGGT CACTGTAAAG 
GGTCCCAAGG GTGAGGAGCG TGTAAAGCAG AAGGACTTAG GGGATGGTGT GTATGGCTTT 
GAATATTACC CTACAATCCC TGGCACATAC ACTGTCACCA TCACATGGGG TGGCCAGAAC 
ATTGGTCGAA GTCCGTTTGA GGTGAAGGTA GGCACTGAGT GTGGCAATCA GAAAGTTCGG 
GCATGGGGTC CTGGCCTGGA AGGAGGCATT GTTGGCAAGT CAGCAGACTT CGTAGTAGAG 
GCCATTGGTG ATGATGTGGG CACCTTGGGT TTCTCTGTGG AAGGTCCATC ACAGGCAAAG 
ATTGAATGTG ACGACAAGGG TGATGGCTCC TGTGATGTGC GCTATTGGCC CCAGGAGGCT 
GGCGAGTATG CTGTTCATGT GCTGTGTAAC AGTGAGGATA TCCGTCTCAG TCCTTTCATG 
GCTGACATCC GTGAGGCACC CCAGGATTTT CACCCAGACA GGGTGAAGGC ACGTGGGCCT 
GGATTGGAGA AGACTGGTGT GGCTGTCAAC AAGCCAGCAG AGTTCACAGT TGATGCCAAG 
CATGCTGGGA AGGCTCCTCT TCGAGTTCAA GTTCAGGACA ATGAGGGCTG CTCTGTGGAA 
GCGACAGTCA AGGACAATGG CAATGGTACT TACAGCTGTT CTTATGTGCC CAGAAAGCCA 
GTGAAGCACA CAGCCATGGT TTCTTGGGGA GGTGTCAGCA TCCCCAACAG TCCTTTCCGG 
GTGAATGTGG GAGCTGGCAG CCATCCAAAC AAAGTCAAGG TGTATGGTCC AGGAGTGGCC 
AAGACTGGGC TCAAGGCCCA TGAACCTACC TACTTTACTG TGGATTGTAC TGAAGCTGGC 
CAGGGAGATG TCAGCATTGG TATCAAGTGT GCCCCTGGAG TAGTGGGCCC CACTGAGGCT 
GATATTGACT TTGATATCAT CCGCAATGAC AATGACACCT TCACTGTAAA ATACACACCC 
TGTGGGGCTG GCAGCTATAC CATCATGGTC CTTTTTGCTG ACCAGGCCAC ACCCACCAGC 
CCCATCAGAG TCAAAGTGGA GCCTTCTCAT GATGCCAGCA AGGTGAAGGC TGAGGGTCCT 
GGCCTAAATC GCACTGGTGT TGAACTTGGC AAACCCACCC ATTTCACAGT CAATGCTAAA 
ACTGCTGGGA AAGGCAAGCT GGATGTCCAA TTCTCAGGAC TGGCTAAGGG AGATGCAGTA 
CGGGATGTGG ACATCATTGA CCACCATGAT AATACCTACA CAGTCAAGTA CATTCCTGTG 
CAGCAGGGCC CAGTAGGTGT CAATGTCACT TATGGAGGAG ATCACATCCC CAAGAGTCCA 
TTTTCAGTGG GAGTATCTCC AAGCCTGGAT CTCAGCAAAA TCAAGGTGTC TGGCCTTGGT 
GACAAAGTGG ACGTTGGCAA AGATCAAGAG TTCACAGTAA AGTCAAAGGG TGCAGGTGGT 
CAAGGCAAAG TAGCATCCAA GATTGTGAGT CCCTCAGGTG CAGCGGTACC CTGCAAGGTA 
GAGCCAGGCC TGGGAGCTGA CAACAGCGTG GTACGTTTTG TGCCCCGTGA AGAGGGGCCC 
TATGAGGTGG AAGTGACCTA TGATGGTGTG CCTGTACCTG GCAGTCCCTT TCCACTAGAA 
GCTGTGGCCC CCACCAAACC CAGCAAGGTG AAGGCGTTTG GACCAGGGCT ACAGGGGGGC 
AATGCAGGCT CCCCTGCCCG CTTCACCATT GATACAAAGG GTGCTGGCAC TGGTGGCCTG 
GGCCTGACAG TGGAAGGCCC CTGTGAAGCA CAGCTTGAGT GCCTAGACAA CGGGGATGGT 
ACATGCTCTG TGTCTTATGT ACCCACTGAG CCTGGGGACT ACAACATCAA CATCCTTTTT 
GCTGACACCC ACATTCCTGG ATCCCCATTC AAGGCCCATG TGGCTCCTTG TTTTGATGCA 
TCCAAGGTGA AGTGCTCAGG CCCTGGGCTG GAGCGGGCTA CTGCTGGTGA GGTAGGGCAG 
TTCCAAGTGG ACTGTTCAAG TGCTGGCAGT GCTGAGTTGA CGATTGAGAT CTGCTCTGAG 
GCAGGACTGC CAGCTGAAGT ATACATTCAA GACCATGGTG ATGGCACACA CACCATTACC 
TATATTCCTC TCTGTCCTGG GGCTTACACT GTTACCATCA AGTATGGCGG CCAGCCTGTG 
CCCAACTTCC CCAGCAAGCT ACAGGTGGAA CCTGCTGTAG ATACCTCAGG TGTACAGTGC 
TATGGGCCTG GGATTGAAGG TCAAGGTGTC TTCCGAGAGG CAACCACTGA GTTCAGTGTG 
GATGCCCGGG CTCTTACACA GACTGGAGGG CCACATGTCA AGGCTCGAGT GGCCAACCCC 
TCAGGCAATC TGACAGATAC CTATGTGCAA GACTGTGGGG ATGGCACATA CAAAGTGGAA 
TACACTCCAT ATGAGGAAGG AGTACACTCT GTGGATGTGA CTTATGATGG CAGCCCTGTG 
CCCAGCAGCC CCTTCCAGGT GCCTGTAACA GAGGGCTGTG ACCCCTCCCG GGTGCGTGTC 
CATGGACCAG GCATCCAAAG TGGTACCACC AACAAACCCA ACAAGTTCAC AGTAGAAACC 
AGGGGAGCTG GCACAGGTGG CCTGGGCTTG GCTGTTGAGG GTCCCTCAGA GGCCAAGATG 
TCTTGTATGG ATAATAAAGA TGGCAGCTGC TCAGTAGAAT ACATCCCCTA TGAAGCTGGA 
ACCTATAGCC TTAATGTCAC TTATGGTGGT CACCAAGTGC CAGGTAGTCC CTTCAAGGTC 
CCTGTACATG ATGTGACAGA TGCATCTAAA GTCAAGTGTT CTGGACCTGG CCTAAGCCCA 
GGCATGGTCC GTGCCAACCT CCCTCAGTCC TTTCAGGTGG ACACAAGCAA AGCTGGAGTT 
GCCCCACTGC AGGTCAAAGT GCAGGGGCCC AAAGGCCTGG TGGAGCCAGT GGATGTAGTG 
GACAATGCTG ATGGTACTCA GACTGTCAAC TATGTGCCCA GCCGAGAAGG GTCCTATAGC 
ATTTCTGTGC TGTATGGTGA AGAAGAAGTG CCACGGAGCC CCTTCAAGGT CAAGGTGCTG 
CCTACACATG ATGCCAGTAA GGTGAAGGCC AGTGGACCTG GACTCAACAC CACTGGTGTA 
CCTGCTAGCC TGCCTGTGGA GTTCACCATT GATGCCAAGG ATGCTGGGGA GGGTCTGTTG 
GCTGTCCAGA TTACGGATCC TGAAGGCAAG CCCAAGAAGA CACACATTCA AGATAATCAT 
GATGGCACAT ACACGGTGGC TTATGTGCCA GATGTGCCAG GCCGGTACAC AATCCTCATC 
AAGTATGGTG GTGATGAGAT TCCCTTTTCC CCGTACCGTG TCCGGGCTGT GCCCACTGGG 
GATGCCAGCA AGTGCACAGT CACAGGTGCT GGCATTGGCC CCACCATCCA GATTGGGGAG 
GAGACGGTGA TTACTGTGGA CACAAAAGCA GCAGGCAAAG GCAAAGTGAC TTGTACTGTG 
TGCACACCTG ATGGCTCAGA GGTAGACGTG GACGTGGTGG AGAATGAGGA TGGCACCTTT 
GACATCTTCT ACACAGCTCC CCAACCGGGC AAATATGTCA TCTGTGTGCG CTTCGGTGGC 
GAGCATGTGC CCAACAGCCC CTTCCAAGTT ACAGCTTTGG CTGGGGACCA ACCAACAGTG 
CAGACCCCAT TAAGGTCTCA GCAGCTGGCT CCACAGTATA ACTATCCTCA GGGTAGCCAG 
CAAACCTGGA TTCCAGAGAG GCCCATGGTG GGCGTTAATG GGCTGGATGT GACCAGCCTG 
AGGCCCTTTG ATCTTGTCAT CCCCTTCACT ATCAAGAAGG GGGAGATCAC TGGGGAAGTT 
CGAATGCCCT CAGGCAAGGT GGCCCAGCCT TCCATTACTG ATAACAAAGA TGGCACTGTT 
ACTGTACGTT ACTCACCCAG TGAAGCTGGC CTGCATGAAA TGGACATTCG CTATGACAAT 
ATGCATATCC CAGGAAGCCC TCTGCAGTTC TATGTTGATT ATGTCAACTG TGGCCACATC 
ACTGCTTATG GTCCTGGCCT TACCCATGGA GTGGTCAACA AACCTGCCAC CTTCACTGTC 
AACACCAAGG ATGCAGGAGA GGGGGGCTTG TCTCTGGCCA TTGAGGGTCC ATCTAAAGCA 
GAAATCAGTT GCACTGACAA CCAGGATGGA ACATGCAGTG TCTCTTACCT GCCTGTACTG 
CCTGGTGACT ATAGCATCCT AGTTAAGTAC AATGATCAAC ACATCCCAGG CAGTCCCTTT 
ACTGCCAGAG TAACAGGTGA CGATTCCATG CGTATGTCCC ACCTAAAGGT GGGTTCTGCT 
GCTGATATCC CCATCAATAT CTCAGAAACA GACCTTAGCC TACTCACAGC CACTGTGGTG 
CCACCTTCGG GTCGAGAGGA ACCCTGTCTG CTGAAACGTT TGCGAAATGG CCACGTGGGG 
ATTTCCTTCG TGCCCAAGGA GACAGGGGAG CACCTGGTAC ATGTGAAGAA GAATGGCCAG 
CATGTGGCAA GCAGTCCCAT CCCAGTAGTG ATCAGCCAGT CGGAGATAGG TGATGCCAGC 
CGTGTGAGGG TCTCTGGTCA AGGTCTTCAT GAAGGTCATA CCTTTGAGCC TGCAGAGTTT 
ATTATTGACA CCAGAGATGC AGGCTACGGT GGGCTTAGTC TGTCCATTGA GGGCCCTAGC 
AAAGTAGACA TCAACACAGA GGATCTGGAG GATGGCACAT GCAGGGTCAC CTACTGTCCC 
ACAGAGCCTG GAAACTACAT TATAAACATC AAATTTGCTG ACCAGCATGT GCCTGGCAGT 
CCCTTTTCTG TGAAGGTGAC AGGTGAGGGC CGGGTGAAAG AGAGTATCAC ACGCAGGCGA 
CGTGCCCCTT CTGTGGCCAA TATTGGCAGT CATTGTGACC TCAGCCTGAA GATTCCTGAA 
ATTAGCATCC AAGATATGAC AGCCCAGGTG ACCAGCCCAT CAGGCAAGAC CCATGAGGCA 
GAGATCGTAG AAGGAGAGAA CCATACTTAC TGTATCCGAT TTGTGCCTGC TGAGATGGGA 
ATGCATACAG TCAGTGTCAA GTACAAGGGC CAGCATGTAC CTGGGAGCCC CTTCCAGTTC 
ACTGTGGGGC CTCTGGGGGA AGGGGGTGCT CACAAGGTCC GTGCTGGAGG CCCTGGCCTA 
GAGAGGGCTG AAGTTGGAGT GCCAGCGGAG TTCGGCATTT GGACTAGGGA AGCTGGCGCT 
GGAGGCCTGG CCATTGCTGT TGAAGGCCCC AGCAAGGCTG AGATCTCTTT CGAGGACCGA 
AAGGATGGCT CCTGTGGTGT GGCCTACGTA GTTCAGGAGC CAGGTGACTA TGAGGTCTCA 
GTCAAGTTCA ACGAGGAGCA CATACCTGAT AGCCCCTTCG TGGTGCCTGT GGCTTCTCCG 
TCTGGTGACG CCCGCCGCCT TACTGTTTCT AGTCTTCAGG AGTCAGGGTT AAAGGTCAAC 
CAGCCAGCAT CTTTTGCAGT CAGTCTGAAT GGAGCCAAGG GGGCAATTGA TGCCAAGGTG 
CACAGCCCCT CAGGAGCTCT GGAGGAGTGC TATGTCACAG AGATTGACCA AGATAAGTAT 
GCTGTGCGTT TCATCCCACG AGAGAATGGC ATCTACTTGA TTGATGTCAA GTTCAATGGT 
ACTCACATTC CTGGAAGTCC CTTCAAGATC CGAGTTGGGG AGCCTGGGCA TGGAGGGGAC 
CCAGGCTTAG TGTCCGCCTA TGGAGCAGGC CTGGAAGGTG GTGTCACAGG GAGCCCAGCA 
GAGTTTATTG TGAACACAAG CAATGCAGGA GCTGGTGCCC TTTCGGTTAC CATTGATGGC 
CCCTCCAAGG TGAAGATGGA TTGCCAGGAG TGCCCCGAGG GCTATCGTGT CACCTATACC 
CCCATGGCAC CTGGCAGCTA CCTCATCTCC ATCAAGTATG GTGGCCCCTA TCACATTGGT 
GGAAGTCCCT TTAAAGCCAA GGTCACAGGT CCTCGTCTTG TAAGCAACCA CAGCCTCCAT 
GAGACATCAT CTGTGTTTGT GGACTCTTTG ACTAAAGTTG CCACGGTTCC CCAGCATGCA 
ACCTCAGGCC CAGGTCCTGC TGATGTCAGC AAGGTAGTAG CCAAAGGCCT GGGGCTAAGC 
AAGGCTTATG TAGGCCAGAA GAGCAACTTC ACAGTAGATT GCAGCAAAGC AGGTAACAAC 
ATGCTGCTGG TGGGCGTGCA TGGCCCAAGG ACACCCTGTG AAGAGATCCT GGTGAAACAC 
ATGGGCAGCC GCCTCTATAG TGTCTCTTAC CTGCTCAAGG ACAAAGGGGA GTACACACTG 
GTGGTCAAGT GGGGTGATGA GCATATCCCA GGCAGCCCAT ACCGCATTAT GGTACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Flna ( XM_011247549.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245851.1 Mus musculus filamin, alpha (Flna), transcript variant X2, mRNA.

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CloneID	OMu20363
Clone ID Related Accession (Same CDS sequence)	XM_011247549.2 , XM_011247549.3 , NM_010227.3
Accession Version	XM_011247549.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	filamin-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCT CTCACTCCCG CTGTGGCCAG AGTGCGGCGG TTGCGTCTCC GGGAGGCAGT 
ATCGATTCAC GGGACGCGGA GATGCCGGCT ACCGAAAAAG ACCTAGCAGA AGATGCACCG 
TGGAAGAAAA TTCAGCAGAA CACATTCACC CGCTGGTGCA ATGAGCACCT TAAGTGCGTA 
AGCAAGCGCA TCGCCAATCT GCAGACGGAC CTGAGCGATG GGTTGCGGCT CATCGCGCTG 
CTCGAGGTAC TCAGCCAGAA GAAAATGCAC CGCAAGCACA ACCAAAGACC CACTTTCCGC 
CAGATGCAGC TCGAAAATGT GTCGGTGGCG CTTGAATTCC TGGACCGTGA GAGCATCAAG 
CTCGTGTCCA TAGACAGCAA GGCTATTGTG GATGGAAATC TGAAGCTGAT CTTAGGCCTC 
ATCTGGACCC TGATCCTGCA CTATTCCATC TCAATGCCCA TGTGGGATGA GGAAGAGGAT 
GAGGAGGCCA AGAAGCAAAC ACCCAAGCAG AGGCTTCTAG GCTGGATTCA GAACAAGCTA 
CCACAGCTTC CCATTACCAA CTTCAGTCGA GACTGGCAGA GTGGCCGGGC CCTGGGTGCT 
CTTGTTGATA GCTGTGCCCC AGGCCTATGT CCTGACTGGG ACTCCTGGGA TGCTAGTAAG 
CCTGTGAACA ATGCACGGGA AGCCATGCAG CAGGCTGATG ACTGGCTAGG CATTCCTCAG 
GTGATTACCC CAGAAGAAAT TGTGGACCCC AATGTAGATG AGCATTCTGT TATGACCTAC 
CTGTCTCAGT TTCCCAAGGC CAAGCTGAAG CCAGGGGCTC CTCTTCGGCC CAAACTGAAC 
CCGAAGAAAG CCCGAGCCTA TGGGCCAGGC ATCGAGCCTA CAGGCAATAT GGTGAAGAAG 
AGAGCAGAAT TCACTGTGGA GACCCGAAGT GCTGGACAGG GAGAAGTGCT TGTATATGTG 
GAGGACCCAG CTGGACACCA GGAAGAGGCA AAAGTGACTG CCAATAATGA CAAGAACCGT 
ACTTTCTCTG TCTGGTATGT CCCTGAAGTG ACAGGGACTC ATAAGGTGAC TGTGCTCTTT 
GCTGGCCAAC ATATTGCCAA GAGCCCCTTT GAGGTGTATG TGGACAAGTC ACAGGGTGAT 
GCCAGCAAAG TGACTGCCCA GGGCCCTGGT CTGGAGCCCA GTGGCAATAT TGCCAACAAG 
ACTACCTACT TTGAGATCTT CACTGCAGGA GCTGGCATGG GTGAGGTGGA AGTTGTCATC 
CAGGACCCTA CAGGACAGAA AGGCACAGTG GAACCTCAGC TGGAGGCCAG GGGTGACAGC 
ACCTATCGCT GTAGCTATCA GCCCACCATG GAGGGTGTCC ATACAGTACA TGTCACCTTC 
GCCGGTGTTC CCATCCCTCG TAGCCCCTAC ACTGTCACTG TTGGCCAAGC CTGTAACCCA 
GCTGCCTGCC GGGCTATTGG TAGAGGCCTT CAGCCCAAGG GTGTTCGAGT GAAGGAAACA 
GCCGACTTCA AGGTGTACAC AAAGGGCGCT GGCAGTGGGG AGCTAAAGGT CACTGTAAAG 
GGTCCCAAGG GTGAGGAGCG TGTAAAGCAG AAGGACTTAG GGGATGGTGT GTATGGCTTT 
GAATATTACC CTACAATCCC TGGCACATAC ACTGTCACCA TCACATGGGG TGGCCAGAAC 
ATTGGTCGAA GTCCGTTTGA GGTGAAGGTA GGCACTGAGT GTGGCAATCA GAAAGTTCGG 
GCATGGGGTC CTGGCCTGGA AGGAGGCATT GTTGGCAAGT CAGCAGACTT CGTAGTAGAG 
GCCATTGGTG ATGATGTGGG CACCTTGGGT TTCTCTGTGG AAGGTCCATC ACAGGCAAAG 
ATTGAATGTG ACGACAAGGG TGATGGCTCC TGTGATGTGC GCTATTGGCC CCAGGAGGCT 
GGCGAGTATG CTGTTCATGT GCTGTGTAAC AGTGAGGATA TCCGTCTCAG TCCTTTCATG 
GCTGACATCC GTGAGGCACC CCAGGATTTT CACCCAGACA GGGTGAAGGC ACGTGGGCCT 
GGATTGGAGA AGACTGGTGT GGCTGTCAAC AAGCCAGCAG AGTTCACAGT TGATGCCAAG 
CATGCTGGGA AGGCTCCTCT TCGAGTTCAA GTTCAGGACA ATGAGGGCTG CTCTGTGGAA 
GCGACAGTCA AGGACAATGG CAATGGTACT TACAGCTGTT CTTATGTGCC CAGAAAGCCA 
GTGAAGCACA CAGCCATGGT TTCTTGGGGA GGTGTCAGCA TCCCCAACAG TCCTTTCCGG 
GTGAATGTGG GAGCTGGCAG CCATCCAAAC AAAGTCAAGG TGTATGGTCC AGGAGTGGCC 
AAGACTGGGC TCAAGGCCCA TGAACCTACC TACTTTACTG TGGATTGTAC TGAAGCTGGC 
CAGGGAGATG TCAGCATTGG TATCAAGTGT GCCCCTGGAG TAGTGGGCCC CACTGAGGCT 
GATATTGACT TTGATATCAT CCGCAATGAC AATGACACCT TCACTGTAAA ATACACACCC 
TGTGGGGCTG GCAGCTATAC CATCATGGTC CTTTTTGCTG ACCAGGCCAC ACCCACCAGC 
CCCATCAGAG TCAAAGTGGA GCCTTCTCAT GATGCCAGCA AGGTGAAGGC TGAGGGTCCT 
GGCCTAAATC GCACTGGTGT TGAACTTGGC AAACCCACCC ATTTCACAGT CAATGCTAAA 
ACTGCTGGGA AAGGCAAGCT GGATGTCCAA TTCTCAGGAC TGGCTAAGGG AGATGCAGTA 
CGGGATGTGG ACATCATTGA CCACCATGAT AATACCTACA CAGTCAAGTA CATTCCTGTG 
CAGCAGGGCC CAGTAGGTGT CAATGTCACT TATGGAGGAG ATCACATCCC CAAGAGTCCA 
TTTTCAGTGG GAGTATCTCC AAGCCTGGAT CTCAGCAAAA TCAAGGTGTC TGGCCTTGGT 
GACAAAGTGG ACGTTGGCAA AGATCAAGAG TTCACAGTAA AGTCAAAGGG TGCAGGTGGT 
CAAGGCAAAG TAGCATCCAA GATTGTGAGT CCCTCAGGTG CAGCGGTACC CTGCAAGGTA 
GAGCCAGGCC TGGGAGCTGA CAACAGCGTG GTACGTTTTG TGCCCCGTGA AGAGGGGCCC 
TATGAGGTGG AAGTGACCTA TGATGGTGTG CCTGTACCTG GCAGTCCCTT TCCACTAGAA 
GCTGTGGCCC CCACCAAACC CAGCAAGGTG AAGGCGTTTG GACCAGGGCT ACAGGGGGGC 
AATGCAGGCT CCCCTGCCCG CTTCACCATT GATACAAAGG GTGCTGGCAC TGGTGGCCTG 
GGCCTGACAG TGGAAGGCCC CTGTGAAGCA CAGCTTGAGT GCCTAGACAA CGGGGATGGT 
ACATGCTCTG TGTCTTATGT ACCCACTGAG CCTGGGGACT ACAACATCAA CATCCTTTTT 
GCTGACACCC ACATTCCTGG ATCCCCATTC AAGGCCCATG TGGCTCCTTG TTTTGATGCA 
TCCAAGGTGA AGTGCTCAGG CCCTGGGCTG GAGCGGGCTA CTGCTGGTGA GGTAGGGCAG 
TTCCAAGTGG ACTGTTCAAG TGCTGGCAGT GCTGAGTTGA CGATTGAGAT CTGCTCTGAG 
GCAGGACTGC CAGCTGAAGT ATACATTCAA GACCATGGTG ATGGCACACA CACCATTACC 
TATATTCCTC TCTGTCCTGG GGCTTACACT GTTACCATCA AGTATGGCGG CCAGCCTGTG 
CCCAACTTCC CCAGCAAGCT ACAGGTGGAA CCTGCTGTAG ATACCTCAGG TGTACAGTGC 
TATGGGCCTG GGATTGAAGG TCAAGGTGTC TTCCGAGAGG CAACCACTGA GTTCAGTGTG 
GATGCCCGGG CTCTTACACA GACTGGAGGG CCACATGTCA AGGCTCGAGT GGCCAACCCC 
TCAGGCAATC TGACAGATAC CTATGTGCAA GACTGTGGGG ATGGCACATA CAAAGTGGAA 
TACACTCCAT ATGAGGAAGG AGTACACTCT GTGGATGTGA CTTATGATGG CAGCCCTGTG 
CCCAGCAGCC CCTTCCAGGT GCCTGTAACA GAGGGCTGTG ACCCCTCCCG GGTGCGTGTC 
CATGGACCAG GCATCCAAAG TGGTACCACC AACAAACCCA ACAAGTTCAC AGTAGAAACC 
AGGGGAGCTG GCACAGGTGG CCTGGGCTTG GCTGTTGAGG GTCCCTCAGA GGCCAAGATG 
TCTTGTATGG ATAATAAAGA TGGCAGCTGC TCAGTAGAAT ACATCCCCTA TGAAGCTGGA 
ACCTATAGCC TTAATGTCAC TTATGGTGGT CACCAAGTGC CAGGTAGTCC CTTCAAGGTC 
CCTGTACATG ATGTGACAGA TGCATCTAAA GTCAAGTGTT CTGGACCTGG CCTAAGCCCA 
GGCATGGTCC GTGCCAACCT CCCTCAGTCC TTTCAGGTGG ACACAAGCAA AGCTGGAGTT 
GCCCCACTGC AGGTCAAAGT GCAGGGGCCC AAAGGCCTGG TGGAGCCAGT GGATGTAGTG 
GACAATGCTG ATGGTACTCA GACTGTCAAC TATGTGCCCA GCCGAGAAGG GTCCTATAGC 
ATTTCTGTGC TGTATGGTGA AGAAGAAGTG CCACGGAGCC CCTTCAAGGT CAAGGTGCTG 
CCTACACATG ATGCCAGTAA GGTGAAGGCC AGTGGACCTG GACTCAACAC CACTGGTGTA 
CCTGCTAGCC TGCCTGTGGA GTTCACCATT GATGCCAAGG ATGCTGGGGA GGGTCTGTTG 
GCTGTCCAGA TTACGGATCC TGAAGGCAAG CCCAAGAAGA CACACATTCA AGATAATCAT 
GATGGCACAT ACACGGTGGC TTATGTGCCA GATGTGCCAG GCCGGTACAC AATCCTCATC 
AAGTATGGTG GTGATGAGAT TCCCTTTTCC CCGTACCGTG TCCGGGCTGT GCCCACTGGG 
GATGCCAGCA AGTGCACAGT CACAGGTGCT GGCATTGGCC CCACCATCCA GATTGGGGAG 
GAGACGGTGA TTACTGTGGA CACAAAAGCA GCAGGCAAAG GCAAAGTGAC TTGTACTGTG 
TGCACACCTG ATGGCTCAGA GGTAGACGTG GACGTGGTGG AGAATGAGGA TGGCACCTTT 
GACATCTTCT ACACAGCTCC CCAACCGGGC AAATATGTCA TCTGTGTGCG CTTCGGTGGC 
GAGCATGTGC CCAACAGCCC CTTCCAAGTT ACAGCTTTGG CTGGGGACCA ACCAACAGTG 
CAGACCCCAT TAAGGTCTCA GCAGCTGGCT CCACAGTATA ACTATCCTCA GGGTAGCCAG 
CAAACCTGGA TTCCAGAGAG GCCCATGGTG GGCGTTAATG GGCTGGATGT GACCAGCCTG 
AGGCCCTTTG ATCTTGTCAT CCCCTTCACT ATCAAGAAGG GGGAGATCAC TGGGGAAGTT 
CGAATGCCCT CAGGCAAGGT GGCCCAGCCT TCCATTACTG ATAACAAAGA TGGCACTGTT 
ACTGTACGTT ACTCACCCAG TGAAGCTGGC CTGCATGAAA TGGACATTCG CTATGACAAT 
ATGCATATCC CAGGAAGCCC TCTGCAGTTC TATGTTGATT ATGTCAACTG TGGCCACATC 
ACTGCTTATG GTCCTGGCCT TACCCATGGA GTGGTCAACA AACCTGCCAC CTTCACTGTC 
AACACCAAGG ATGCAGGAGA GGGGGGCTTG TCTCTGGCCA TTGAGGGTCC ATCTAAAGCA 
GAAATCAGTT GCACTGACAA CCAGGATGGA ACATGCAGTG TCTCTTACCT GCCTGTACTG 
CCTGGTGACT ATAGCATCCT AGTTAAGTAC AATGATCAAC ACATCCCAGG CAGTCCCTTT 
ACTGCCAGAG TAACAGGTGA CGATTCCATG CGTATGTCCC ACCTAAAGGT GGGTTCTGCT 
GCTGATATCC CCATCAATAT CTCAGAAACA GACCTTAGCC TACTCACAGC CACTGTGGTG 
CCACCTTCGG GTCGAGAGGA ACCCTGTCTG CTGAAACGTT TGCGAAATGG CCACGTGGGG 
ATTTCCTTCG TGCCCAAGGA GACAGGGGAG CACCTGGTAC ATGTGAAGAA GAATGGCCAG 
CATGTGGCAA GCAGTCCCAT CCCAGTAGTG ATCAGCCAGT CGGAGATAGG TGATGCCAGC 
CGTGTGAGGG TCTCTGGTCA AGGTCTTCAT GAAGGTCATA CCTTTGAGCC TGCAGAGTTT 
ATTATTGACA CCAGAGATGC AGGCTACGGT GGGCTTAGTC TGTCCATTGA GGGCCCTAGC 
AAAGTAGACA TCAACACAGA GGATCTGGAG GATGGCACAT GCAGGGTCAC CTACTGTCCC 
ACAGAGCCTG GAAACTACAT TATAAACATC AAATTTGCTG ACCAGCATGT GCCTGGCAGT 
CCCTTTTCTG TGAAGGTGAC AGGTGAGGGC CGGGTGAAAG AGAGTATCAC ACGCAGGCGA 
CGTGCCCCTT CTGTGGCCAA TATTGGCAGT CATTGTGACC TCAGCCTGAA GATTCCTGAA 
ATTAGCATCC AAGATATGAC AGCCCAGGTG ACCAGCCCAT CAGGCAAGAC CCATGAGGCA 
GAGATCGTAG AAGGAGAGAA CCATACTTAC TGTATCCGAT TTGTGCCTGC TGAGATGGGA 
ATGCATACAG TCAGTGTCAA GTACAAGGGC CAGCATGTAC CTGGGAGCCC CTTCCAGTTC 
ACTGTGGGGC CTCTGGGGGA AGGGGGTGCT CACAAGGTCC GTGCTGGAGG CCCTGGCCTA 
GAGAGGGCTG AAGTTGGAGT GCCAGCGGAG TTCGGCATTT GGACTAGGGA AGCTGGCGCT 
GGAGGCCTGG CCATTGCTGT TGAAGGCCCC AGCAAGGCTG AGATCTCTTT CGAGGACCGA 
AAGGATGGCT CCTGTGGTGT GGCCTACGTA GTTCAGGAGC CAGGTGACTA TGAGGTCTCA 
GTCAAGTTCA ACGAGGAGCA CATACCTGAT AGCCCCTTCG TGGTGCCTGT GGCTTCTCCG 
TCTGGTGACG CCCGCCGCCT TACTGTTTCT AGTCTTCAGG AGTCAGGGTT AAAGGTCAAC 
CAGCCAGCAT CTTTTGCAGT CAGTCTGAAT GGAGCCAAGG GGGCAATTGA TGCCAAGGTG 
CACAGCCCCT CAGGAGCTCT GGAGGAGTGC TATGTCACAG AGATTGACCA AGATAAGTAT 
GCTGTGCGTT TCATCCCACG AGAGAATGGC ATCTACTTGA TTGATGTCAA GTTCAATGGT 
ACTCACATTC CTGGAAGTCC CTTCAAGATC CGAGTTGGGG AGCCTGGGCA TGGAGGGGAC 
CCAGGCTTAG TGTCCGCCTA TGGAGCAGGC CTGGAAGGTG GTGTCACAGG GAGCCCAGCA 
GAGTTTATTG TGAACACAAG CAATGCAGGA GCTGGTGCCC TTTCGGTTAC CATTGATGGC 
CCCTCCAAGG TGAAGATGGA TTGCCAGGAG TGCCCCGAGG GCTATCGTGT CACCTATACC 
CCCATGGCAC CTGGCAGCTA CCTCATCTCC ATCAAGTATG GTGGCCCCTA TCACATTGGT 
GGAAGTCCCT TTAAAGCCAA GGTCACAGGT CCTCGTCTTG TAAGCAACCA CAGCCTCCAT 
GAGACATCAT CTGTGTTTGT GGACTCTTTG ACTAAAGTTG CCACGGTTCC CCAGCATGCA 
ACCTCAGGCC CAGGTCCTGC TGATGTCAGC AAGGTAGTAG CCAAAGGCCT GGGGCTAAGC 
AAGGCTTATG TAGGCCAGAA GAGCAACTTC ACAGTAGATT GCAGCAAAGC AGGTAACAAC 
ATGCTGCTGG TGGGCGTGCA TGGCCCAAGG ACACCCTGTG AAGAGATCCT GGTGAAACAC 
ATGGGCAGCC GCCTCTATAG TGTCTCTTAC CTGCTCAAGG ACAAAGGGGA GTACACACTG 
GTGGTCAAGT GGGGTGATGA GCATATCCCA GGCAGCCCAT ACCGCATTAT GGTACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245851.1
CDS	240..8159
Translation	MSSSHSRCGQSAAVASPGGSIDSRDAEMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVSKRIANLQTDLSDGLRLIALLEVLSQKKMHRKHNQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEDEEAKKQTPKQRLLGWIQNKLPQLPITNFSRDWQSGRALGALVDSCAPGLCPDWDSWDASKPVNNAREAMQQADDWLGIPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNMVKKRAEFTVETRSAGQGEVLVYVEDPAGHQEEAKVTANNDKNRTFSVWYVPEVTGTHKVTVLFAGQHIAKSPFEVYVDKSQGDASKVTAQGPGLEPSGNIANKTTYFEIFTAGAGMGEVEVVIQDPTGQKGTVEPQLEARGDSTYRCSYQPTMEGVHTVHVTFAGVPIPRSPYTVTVGQACNPAACRAIGRGLQPKGVRVKETADFKVYTKGAGSGELKVTVKGPKGEERVKQKDLGDGVYGFEYYPTIPGTYTVTITWGGQNIGRSPFEVKVGTECGNQKVRAWGPGLEGGIVGKSADFVVEAIGDDVGTLGFSVEGPSQAKIECDDKGDGSCDVRYWPQEAGEYAVHVLCNSEDIRLSPFMADIREAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAKHAGKAPLRVQVQDNEGCSVEATVKDNGNGTYSCSYVPRKPVKHTAMVSWGGVSIPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCTEAGQGDVSIGIKCAPGVVGPTEADIDFDIIRNDNDTFTVKYTPCGAGSYTIMVLFADQATPTSPIRVKVEPSHDASKVKAEGPGLNRTGVELGKPTHFTVNAKTAGKGKLDVQFSGLAKGDAVRDVDIIDHHDNTYTVKYIPVQQGPVGVNVTYGGDHIPKSPFSVGVSPSLDLSKIKVSGLGDKVDVGKDQEFTVKSKGAGGQGKVASKIVSPSGAAVPCKVEPGLGADNSVVRFVPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQGGNAGSPARFTIDTKGAGTGGLGLTVEGPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVAPCFDASKVKCSGPGLERATAGEVGQFQVDCSSAGSAELTIEICSEAGLPAEVYIQDHGDGTHTITYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTGGPHVKARVANPSGNLTDTYVQDCGDGTYKVEYTPYEEGVHSVDVTYDGSPVPSSPFQVPVTEGCDPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKMSCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDVVDNADGTQTVNYVPSREGSYSISVLYGEEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVPDVPGRYTILIKYGGDEIPFSPYRVRAVPTGDASKCTVTGAGIGPTIQIGEETVITVDTKAAGKGKVTCTVCTPDGSEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPTVQTPLRSQQLAPQYNYPQGSQQTWIPERPMVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPSITDNKDGTVTVRYSPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHITAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNDQHIPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANIGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGMHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEVGVPAEFGIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGIYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGSPAEFIVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKVATVPQHATSGPGPADVSKVVAKGLGLSKAYVGQKSNFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHMGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRIMVP

Target ORF information:

RefSeq Version	XM_011247549.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus filamin, alpha (Flna), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247549.2

ORF Insert Sequence:

ATGAGTAGCT CTCACTCCCG CTGTGGCCAG AGTGCGGCGG TTGCGTCTCC GGGAGGCAGT 
ATCGATTCAC GGGACGCGGA GATGCCGGCT ACCGAAAAAG ACCTAGCAGA AGATGCACCG 
TGGAAGAAAA TTCAGCAGAA CACATTCACC CGCTGGTGCA ATGAGCACCT TAAGTGCGTA 
AGCAAGCGCA TCGCCAATCT GCAGACGGAC CTGAGCGATG GGTTGCGGCT CATCGCGCTG 
CTCGAGGTAC TCAGCCAGAA GAAAATGCAC CGCAAGCACA ACCAAAGACC CACTTTCCGC 
CAGATGCAGC TCGAAAATGT GTCGGTGGCG CTTGAATTCC TGGACCGTGA GAGCATCAAG 
CTCGTGTCCA TAGACAGCAA GGCTATTGTG GATGGAAATC TGAAGCTGAT CTTAGGCCTC 
ATCTGGACCC TGATCCTGCA CTATTCCATC TCAATGCCCA TGTGGGATGA GGAAGAGGAT 
GAGGAGGCCA AGAAGCAAAC ACCCAAGCAG AGGCTTCTAG GCTGGATTCA GAACAAGCTA 
CCACAGCTTC CCATTACCAA CTTCAGTCGA GACTGGCAGA GTGGCCGGGC CCTGGGTGCT 
CTTGTTGATA GCTGTGCCCC AGGCCTATGT CCTGACTGGG ACTCCTGGGA TGCTAGTAAG 
CCTGTGAACA ATGCACGGGA AGCCATGCAG CAGGCTGATG ACTGGCTAGG CATTCCTCAG 
GTGATTACCC CAGAAGAAAT TGTGGACCCC AATGTAGATG AGCATTCTGT TATGACCTAC 
CTGTCTCAGT TTCCCAAGGC CAAGCTGAAG CCAGGGGCTC CTCTTCGGCC CAAACTGAAC 
CCGAAGAAAG CCCGAGCCTA TGGGCCAGGC ATCGAGCCTA CAGGCAATAT GGTGAAGAAG 
AGAGCAGAAT TCACTGTGGA GACCCGAAGT GCTGGACAGG GAGAAGTGCT TGTATATGTG 
GAGGACCCAG CTGGACACCA GGAAGAGGCA AAAGTGACTG CCAATAATGA CAAGAACCGT 
ACTTTCTCTG TCTGGTATGT CCCTGAAGTG ACAGGGACTC ATAAGGTGAC TGTGCTCTTT 
GCTGGCCAAC ATATTGCCAA GAGCCCCTTT GAGGTGTATG TGGACAAGTC ACAGGGTGAT 
GCCAGCAAAG TGACTGCCCA GGGCCCTGGT CTGGAGCCCA GTGGCAATAT TGCCAACAAG 
ACTACCTACT TTGAGATCTT CACTGCAGGA GCTGGCATGG GTGAGGTGGA AGTTGTCATC 
CAGGACCCTA CAGGACAGAA AGGCACAGTG GAACCTCAGC TGGAGGCCAG GGGTGACAGC 
ACCTATCGCT GTAGCTATCA GCCCACCATG GAGGGTGTCC ATACAGTACA TGTCACCTTC 
GCCGGTGTTC CCATCCCTCG TAGCCCCTAC ACTGTCACTG TTGGCCAAGC CTGTAACCCA 
GCTGCCTGCC GGGCTATTGG TAGAGGCCTT CAGCCCAAGG GTGTTCGAGT GAAGGAAACA 
GCCGACTTCA AGGTGTACAC AAAGGGCGCT GGCAGTGGGG AGCTAAAGGT CACTGTAAAG 
GGTCCCAAGG GTGAGGAGCG TGTAAAGCAG AAGGACTTAG GGGATGGTGT GTATGGCTTT 
GAATATTACC CTACAATCCC TGGCACATAC ACTGTCACCA TCACATGGGG TGGCCAGAAC 
ATTGGTCGAA GTCCGTTTGA GGTGAAGGTA GGCACTGAGT GTGGCAATCA GAAAGTTCGG 
GCATGGGGTC CTGGCCTGGA AGGAGGCATT GTTGGCAAGT CAGCAGACTT CGTAGTAGAG 
GCCATTGGTG ATGATGTGGG CACCTTGGGT TTCTCTGTGG AAGGTCCATC ACAGGCAAAG 
ATTGAATGTG ACGACAAGGG TGATGGCTCC TGTGATGTGC GCTATTGGCC CCAGGAGGCT 
GGCGAGTATG CTGTTCATGT GCTGTGTAAC AGTGAGGATA TCCGTCTCAG TCCTTTCATG 
GCTGACATCC GTGAGGCACC CCAGGATTTT CACCCAGACA GGGTGAAGGC ACGTGGGCCT 
GGATTGGAGA AGACTGGTGT GGCTGTCAAC AAGCCAGCAG AGTTCACAGT TGATGCCAAG 
CATGCTGGGA AGGCTCCTCT TCGAGTTCAA GTTCAGGACA ATGAGGGCTG CTCTGTGGAA 
GCGACAGTCA AGGACAATGG CAATGGTACT TACAGCTGTT CTTATGTGCC CAGAAAGCCA 
GTGAAGCACA CAGCCATGGT TTCTTGGGGA GGTGTCAGCA TCCCCAACAG TCCTTTCCGG 
GTGAATGTGG GAGCTGGCAG CCATCCAAAC AAAGTCAAGG TGTATGGTCC AGGAGTGGCC 
AAGACTGGGC TCAAGGCCCA TGAACCTACC TACTTTACTG TGGATTGTAC TGAAGCTGGC 
CAGGGAGATG TCAGCATTGG TATCAAGTGT GCCCCTGGAG TAGTGGGCCC CACTGAGGCT 
GATATTGACT TTGATATCAT CCGCAATGAC AATGACACCT TCACTGTAAA ATACACACCC 
TGTGGGGCTG GCAGCTATAC CATCATGGTC CTTTTTGCTG ACCAGGCCAC ACCCACCAGC 
CCCATCAGAG TCAAAGTGGA GCCTTCTCAT GATGCCAGCA AGGTGAAGGC TGAGGGTCCT 
GGCCTAAATC GCACTGGTGT TGAACTTGGC AAACCCACCC ATTTCACAGT CAATGCTAAA 
ACTGCTGGGA AAGGCAAGCT GGATGTCCAA TTCTCAGGAC TGGCTAAGGG AGATGCAGTA 
CGGGATGTGG ACATCATTGA CCACCATGAT AATACCTACA CAGTCAAGTA CATTCCTGTG 
CAGCAGGGCC CAGTAGGTGT CAATGTCACT TATGGAGGAG ATCACATCCC CAAGAGTCCA 
TTTTCAGTGG GAGTATCTCC AAGCCTGGAT CTCAGCAAAA TCAAGGTGTC TGGCCTTGGT 
GACAAAGTGG ACGTTGGCAA AGATCAAGAG TTCACAGTAA AGTCAAAGGG TGCAGGTGGT 
CAAGGCAAAG TAGCATCCAA GATTGTGAGT CCCTCAGGTG CAGCGGTACC CTGCAAGGTA 
GAGCCAGGCC TGGGAGCTGA CAACAGCGTG GTACGTTTTG TGCCCCGTGA AGAGGGGCCC 
TATGAGGTGG AAGTGACCTA TGATGGTGTG CCTGTACCTG GCAGTCCCTT TCCACTAGAA 
GCTGTGGCCC CCACCAAACC CAGCAAGGTG AAGGCGTTTG GACCAGGGCT ACAGGGGGGC 
AATGCAGGCT CCCCTGCCCG CTTCACCATT GATACAAAGG GTGCTGGCAC TGGTGGCCTG 
GGCCTGACAG TGGAAGGCCC CTGTGAAGCA CAGCTTGAGT GCCTAGACAA CGGGGATGGT 
ACATGCTCTG TGTCTTATGT ACCCACTGAG CCTGGGGACT ACAACATCAA CATCCTTTTT 
GCTGACACCC ACATTCCTGG ATCCCCATTC AAGGCCCATG TGGCTCCTTG TTTTGATGCA 
TCCAAGGTGA AGTGCTCAGG CCCTGGGCTG GAGCGGGCTA CTGCTGGTGA GGTAGGGCAG 
TTCCAAGTGG ACTGTTCAAG TGCTGGCAGT GCTGAGTTGA CGATTGAGAT CTGCTCTGAG 
GCAGGACTGC CAGCTGAAGT ATACATTCAA GACCATGGTG ATGGCACACA CACCATTACC 
TATATTCCTC TCTGTCCTGG GGCTTACACT GTTACCATCA AGTATGGCGG CCAGCCTGTG 
CCCAACTTCC CCAGCAAGCT ACAGGTGGAA CCTGCTGTAG ATACCTCAGG TGTACAGTGC 
TATGGGCCTG GGATTGAAGG TCAAGGTGTC TTCCGAGAGG CAACCACTGA GTTCAGTGTG 
GATGCCCGGG CTCTTACACA GACTGGAGGG CCACATGTCA AGGCTCGAGT GGCCAACCCC 
TCAGGCAATC TGACAGATAC CTATGTGCAA GACTGTGGGG ATGGCACATA CAAAGTGGAA 
TACACTCCAT ATGAGGAAGG AGTACACTCT GTGGATGTGA CTTATGATGG CAGCCCTGTG 
CCCAGCAGCC CCTTCCAGGT GCCTGTAACA GAGGGCTGTG ACCCCTCCCG GGTGCGTGTC 
CATGGACCAG GCATCCAAAG TGGTACCACC AACAAACCCA ACAAGTTCAC AGTAGAAACC 
AGGGGAGCTG GCACAGGTGG CCTGGGCTTG GCTGTTGAGG GTCCCTCAGA GGCCAAGATG 
TCTTGTATGG ATAATAAAGA TGGCAGCTGC TCAGTAGAAT ACATCCCCTA TGAAGCTGGA 
ACCTATAGCC TTAATGTCAC TTATGGTGGT CACCAAGTGC CAGGTAGTCC CTTCAAGGTC 
CCTGTACATG ATGTGACAGA TGCATCTAAA GTCAAGTGTT CTGGACCTGG CCTAAGCCCA 
GGCATGGTCC GTGCCAACCT CCCTCAGTCC TTTCAGGTGG ACACAAGCAA AGCTGGAGTT 
GCCCCACTGC AGGTCAAAGT GCAGGGGCCC AAAGGCCTGG TGGAGCCAGT GGATGTAGTG 
GACAATGCTG ATGGTACTCA GACTGTCAAC TATGTGCCCA GCCGAGAAGG GTCCTATAGC 
ATTTCTGTGC TGTATGGTGA AGAAGAAGTG CCACGGAGCC CCTTCAAGGT CAAGGTGCTG 
CCTACACATG ATGCCAGTAA GGTGAAGGCC AGTGGACCTG GACTCAACAC CACTGGTGTA 
CCTGCTAGCC TGCCTGTGGA GTTCACCATT GATGCCAAGG ATGCTGGGGA GGGTCTGTTG 
GCTGTCCAGA TTACGGATCC TGAAGGCAAG CCCAAGAAGA CACACATTCA AGATAATCAT 
GATGGCACAT ACACGGTGGC TTATGTGCCA GATGTGCCAG GCCGGTACAC AATCCTCATC 
AAGTATGGTG GTGATGAGAT TCCCTTTTCC CCGTACCGTG TCCGGGCTGT GCCCACTGGG 
GATGCCAGCA AGTGCACAGT CACAGGTGCT GGCATTGGCC CCACCATCCA GATTGGGGAG 
GAGACGGTGA TTACTGTGGA CACAAAAGCA GCAGGCAAAG GCAAAGTGAC TTGTACTGTG 
TGCACACCTG ATGGCTCAGA GGTAGACGTG GACGTGGTGG AGAATGAGGA TGGCACCTTT 
GACATCTTCT ACACAGCTCC CCAACCGGGC AAATATGTCA TCTGTGTGCG CTTCGGTGGC 
GAGCATGTGC CCAACAGCCC CTTCCAAGTT ACAGCTTTGG CTGGGGACCA ACCAACAGTG 
CAGACCCCAT TAAGGTCTCA GCAGCTGGCT CCACAGTATA ACTATCCTCA GGGTAGCCAG 
CAAACCTGGA TTCCAGAGAG GCCCATGGTG GGCGTTAATG GGCTGGATGT GACCAGCCTG 
AGGCCCTTTG ATCTTGTCAT CCCCTTCACT ATCAAGAAGG GGGAGATCAC TGGGGAAGTT 
CGAATGCCCT CAGGCAAGGT GGCCCAGCCT TCCATTACTG ATAACAAAGA TGGCACTGTT 
ACTGTACGTT ACTCACCCAG TGAAGCTGGC CTGCATGAAA TGGACATTCG CTATGACAAT 
ATGCATATCC CAGGAAGCCC TCTGCAGTTC TATGTTGATT ATGTCAACTG TGGCCACATC 
ACTGCTTATG GTCCTGGCCT TACCCATGGA GTGGTCAACA AACCTGCCAC CTTCACTGTC 
AACACCAAGG ATGCAGGAGA GGGGGGCTTG TCTCTGGCCA TTGAGGGTCC ATCTAAAGCA 
GAAATCAGTT GCACTGACAA CCAGGATGGA ACATGCAGTG TCTCTTACCT GCCTGTACTG 
CCTGGTGACT ATAGCATCCT AGTTAAGTAC AATGATCAAC ACATCCCAGG CAGTCCCTTT 
ACTGCCAGAG TAACAGGTGA CGATTCCATG CGTATGTCCC ACCTAAAGGT GGGTTCTGCT 
GCTGATATCC CCATCAATAT CTCAGAAACA GACCTTAGCC TACTCACAGC CACTGTGGTG 
CCACCTTCGG GTCGAGAGGA ACCCTGTCTG CTGAAACGTT TGCGAAATGG CCACGTGGGG 
ATTTCCTTCG TGCCCAAGGA GACAGGGGAG CACCTGGTAC ATGTGAAGAA GAATGGCCAG 
CATGTGGCAA GCAGTCCCAT CCCAGTAGTG ATCAGCCAGT CGGAGATAGG TGATGCCAGC 
CGTGTGAGGG TCTCTGGTCA AGGTCTTCAT GAAGGTCATA CCTTTGAGCC TGCAGAGTTT 
ATTATTGACA CCAGAGATGC AGGCTACGGT GGGCTTAGTC TGTCCATTGA GGGCCCTAGC 
AAAGTAGACA TCAACACAGA GGATCTGGAG GATGGCACAT GCAGGGTCAC CTACTGTCCC 
ACAGAGCCTG GAAACTACAT TATAAACATC AAATTTGCTG ACCAGCATGT GCCTGGCAGT 
CCCTTTTCTG TGAAGGTGAC AGGTGAGGGC CGGGTGAAAG AGAGTATCAC ACGCAGGCGA 
CGTGCCCCTT CTGTGGCCAA TATTGGCAGT CATTGTGACC TCAGCCTGAA GATTCCTGAA 
ATTAGCATCC AAGATATGAC AGCCCAGGTG ACCAGCCCAT CAGGCAAGAC CCATGAGGCA 
GAGATCGTAG AAGGAGAGAA CCATACTTAC TGTATCCGAT TTGTGCCTGC TGAGATGGGA 
ATGCATACAG TCAGTGTCAA GTACAAGGGC CAGCATGTAC CTGGGAGCCC CTTCCAGTTC 
ACTGTGGGGC CTCTGGGGGA AGGGGGTGCT CACAAGGTCC GTGCTGGAGG CCCTGGCCTA 
GAGAGGGCTG AAGTTGGAGT GCCAGCGGAG TTCGGCATTT GGACTAGGGA AGCTGGCGCT 
GGAGGCCTGG CCATTGCTGT TGAAGGCCCC AGCAAGGCTG AGATCTCTTT CGAGGACCGA 
AAGGATGGCT CCTGTGGTGT GGCCTACGTA GTTCAGGAGC CAGGTGACTA TGAGGTCTCA 
GTCAAGTTCA ACGAGGAGCA CATACCTGAT AGCCCCTTCG TGGTGCCTGT GGCTTCTCCG 
TCTGGTGACG CCCGCCGCCT TACTGTTTCT AGTCTTCAGG AGTCAGGGTT AAAGGTCAAC 
CAGCCAGCAT CTTTTGCAGT CAGTCTGAAT GGAGCCAAGG GGGCAATTGA TGCCAAGGTG 
CACAGCCCCT CAGGAGCTCT GGAGGAGTGC TATGTCACAG AGATTGACCA AGATAAGTAT 
GCTGTGCGTT TCATCCCACG AGAGAATGGC ATCTACTTGA TTGATGTCAA GTTCAATGGT 
ACTCACATTC CTGGAAGTCC CTTCAAGATC CGAGTTGGGG AGCCTGGGCA TGGAGGGGAC 
CCAGGCTTAG TGTCCGCCTA TGGAGCAGGC CTGGAAGGTG GTGTCACAGG GAGCCCAGCA 
GAGTTTATTG TGAACACAAG CAATGCAGGA GCTGGTGCCC TTTCGGTTAC CATTGATGGC 
CCCTCCAAGG TGAAGATGGA TTGCCAGGAG TGCCCCGAGG GCTATCGTGT CACCTATACC 
CCCATGGCAC CTGGCAGCTA CCTCATCTCC ATCAAGTATG GTGGCCCCTA TCACATTGGT 
GGAAGTCCCT TTAAAGCCAA GGTCACAGGT CCTCGTCTTG TAAGCAACCA CAGCCTCCAT 
GAGACATCAT CTGTGTTTGT GGACTCTTTG ACTAAAGTTG CCACGGTTCC CCAGCATGCA 
ACCTCAGGCC CAGGTCCTGC TGATGTCAGC AAGGTAGTAG CCAAAGGCCT GGGGCTAAGC 
AAGGCTTATG TAGGCCAGAA GAGCAACTTC ACAGTAGATT GCAGCAAAGC AGGTAACAAC 
ATGCTGCTGG TGGGCGTGCA TGGCCCAAGG ACACCCTGTG AAGAGATCCT GGTGAAACAC 
ATGGGCAGCC GCCTCTATAG TGTCTCTTAC CTGCTCAAGG ACAAAGGGGA GTACACACTG 
GTGGTCAAGT GGGGTGATGA GCATATCCCA GGCAGCCCAT ACCGCATTAT GGTACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Flna ( XM_011247549.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245851.1 Mus musculus filamin, alpha (Flna), transcript variant X2, mRNA.

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CloneID	OMu20363
Clone ID Related Accession (Same CDS sequence)	XM_011247549.2 , XM_011247549.3 , NM_010227.3
Accession Version	XM_011247549.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	filamin-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247549.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCT CTCACTCCCG CTGTGGCCAG AGTGCGGCGG TTGCGTCTCC GGGAGGCAGT 
ATCGATTCAC GGGACGCGGA GATGCCGGCT ACCGAAAAAG ACCTAGCAGA AGATGCACCG 
TGGAAGAAAA TTCAGCAGAA CACATTCACC CGCTGGTGCA ATGAGCACCT TAAGTGCGTA 
AGCAAGCGCA TCGCCAATCT GCAGACGGAC CTGAGCGATG GGTTGCGGCT CATCGCGCTG 
CTCGAGGTAC TCAGCCAGAA GAAAATGCAC CGCAAGCACA ACCAAAGACC CACTTTCCGC 
CAGATGCAGC TCGAAAATGT GTCGGTGGCG CTTGAATTCC TGGACCGTGA GAGCATCAAG 
CTCGTGTCCA TAGACAGCAA GGCTATTGTG GATGGAAATC TGAAGCTGAT CTTAGGCCTC 
ATCTGGACCC TGATCCTGCA CTATTCCATC TCAATGCCCA TGTGGGATGA GGAAGAGGAT 
GAGGAGGCCA AGAAGCAAAC ACCCAAGCAG AGGCTTCTAG GCTGGATTCA GAACAAGCTA 
CCACAGCTTC CCATTACCAA CTTCAGTCGA GACTGGCAGA GTGGCCGGGC CCTGGGTGCT 
CTTGTTGATA GCTGTGCCCC AGGCCTATGT CCTGACTGGG ACTCCTGGGA TGCTAGTAAG 
CCTGTGAACA ATGCACGGGA AGCCATGCAG CAGGCTGATG ACTGGCTAGG CATTCCTCAG 
GTGATTACCC CAGAAGAAAT TGTGGACCCC AATGTAGATG AGCATTCTGT TATGACCTAC 
CTGTCTCAGT TTCCCAAGGC CAAGCTGAAG CCAGGGGCTC CTCTTCGGCC CAAACTGAAC 
CCGAAGAAAG CCCGAGCCTA TGGGCCAGGC ATCGAGCCTA CAGGCAATAT GGTGAAGAAG 
AGAGCAGAAT TCACTGTGGA GACCCGAAGT GCTGGACAGG GAGAAGTGCT TGTATATGTG 
GAGGACCCAG CTGGACACCA GGAAGAGGCA AAAGTGACTG CCAATAATGA CAAGAACCGT 
ACTTTCTCTG TCTGGTATGT CCCTGAAGTG ACAGGGACTC ATAAGGTGAC TGTGCTCTTT 
GCTGGCCAAC ATATTGCCAA GAGCCCCTTT GAGGTGTATG TGGACAAGTC ACAGGGTGAT 
GCCAGCAAAG TGACTGCCCA GGGCCCTGGT CTGGAGCCCA GTGGCAATAT TGCCAACAAG 
ACTACCTACT TTGAGATCTT CACTGCAGGA GCTGGCATGG GTGAGGTGGA AGTTGTCATC 
CAGGACCCTA CAGGACAGAA AGGCACAGTG GAACCTCAGC TGGAGGCCAG GGGTGACAGC 
ACCTATCGCT GTAGCTATCA GCCCACCATG GAGGGTGTCC ATACAGTACA TGTCACCTTC 
GCCGGTGTTC CCATCCCTCG TAGCCCCTAC ACTGTCACTG TTGGCCAAGC CTGTAACCCA 
GCTGCCTGCC GGGCTATTGG TAGAGGCCTT CAGCCCAAGG GTGTTCGAGT GAAGGAAACA 
GCCGACTTCA AGGTGTACAC AAAGGGCGCT GGCAGTGGGG AGCTAAAGGT CACTGTAAAG 
GGTCCCAAGG GTGAGGAGCG TGTAAAGCAG AAGGACTTAG GGGATGGTGT GTATGGCTTT 
GAATATTACC CTACAATCCC TGGCACATAC ACTGTCACCA TCACATGGGG TGGCCAGAAC 
ATTGGTCGAA GTCCGTTTGA GGTGAAGGTA GGCACTGAGT GTGGCAATCA GAAAGTTCGG 
GCATGGGGTC CTGGCCTGGA AGGAGGCATT GTTGGCAAGT CAGCAGACTT CGTAGTAGAG 
GCCATTGGTG ATGATGTGGG CACCTTGGGT TTCTCTGTGG AAGGTCCATC ACAGGCAAAG 
ATTGAATGTG ACGACAAGGG TGATGGCTCC TGTGATGTGC GCTATTGGCC CCAGGAGGCT 
GGCGAGTATG CTGTTCATGT GCTGTGTAAC AGTGAGGATA TCCGTCTCAG TCCTTTCATG 
GCTGACATCC GTGAGGCACC CCAGGATTTT CACCCAGACA GGGTGAAGGC ACGTGGGCCT 
GGATTGGAGA AGACTGGTGT GGCTGTCAAC AAGCCAGCAG AGTTCACAGT TGATGCCAAG 
CATGCTGGGA AGGCTCCTCT TCGAGTTCAA GTTCAGGACA ATGAGGGCTG CTCTGTGGAA 
GCGACAGTCA AGGACAATGG CAATGGTACT TACAGCTGTT CTTATGTGCC CAGAAAGCCA 
GTGAAGCACA CAGCCATGGT TTCTTGGGGA GGTGTCAGCA TCCCCAACAG TCCTTTCCGG 
GTGAATGTGG GAGCTGGCAG CCATCCAAAC AAAGTCAAGG TGTATGGTCC AGGAGTGGCC 
AAGACTGGGC TCAAGGCCCA TGAACCTACC TACTTTACTG TGGATTGTAC TGAAGCTGGC 
CAGGGAGATG TCAGCATTGG TATCAAGTGT GCCCCTGGAG TAGTGGGCCC CACTGAGGCT 
GATATTGACT TTGATATCAT CCGCAATGAC AATGACACCT TCACTGTAAA ATACACACCC 
TGTGGGGCTG GCAGCTATAC CATCATGGTC CTTTTTGCTG ACCAGGCCAC ACCCACCAGC 
CCCATCAGAG TCAAAGTGGA GCCTTCTCAT GATGCCAGCA AGGTGAAGGC TGAGGGTCCT 
GGCCTAAATC GCACTGGTGT TGAACTTGGC AAACCCACCC ATTTCACAGT CAATGCTAAA 
ACTGCTGGGA AAGGCAAGCT GGATGTCCAA TTCTCAGGAC TGGCTAAGGG AGATGCAGTA 
CGGGATGTGG ACATCATTGA CCACCATGAT AATACCTACA CAGTCAAGTA CATTCCTGTG 
CAGCAGGGCC CAGTAGGTGT CAATGTCACT TATGGAGGAG ATCACATCCC CAAGAGTCCA 
TTTTCAGTGG GAGTATCTCC AAGCCTGGAT CTCAGCAAAA TCAAGGTGTC TGGCCTTGGT 
GACAAAGTGG ACGTTGGCAA AGATCAAGAG TTCACAGTAA AGTCAAAGGG TGCAGGTGGT 
CAAGGCAAAG TAGCATCCAA GATTGTGAGT CCCTCAGGTG CAGCGGTACC CTGCAAGGTA 
GAGCCAGGCC TGGGAGCTGA CAACAGCGTG GTACGTTTTG TGCCCCGTGA AGAGGGGCCC 
TATGAGGTGG AAGTGACCTA TGATGGTGTG CCTGTACCTG GCAGTCCCTT TCCACTAGAA 
GCTGTGGCCC CCACCAAACC CAGCAAGGTG AAGGCGTTTG GACCAGGGCT ACAGGGGGGC 
AATGCAGGCT CCCCTGCCCG CTTCACCATT GATACAAAGG GTGCTGGCAC TGGTGGCCTG 
GGCCTGACAG TGGAAGGCCC CTGTGAAGCA CAGCTTGAGT GCCTAGACAA CGGGGATGGT 
ACATGCTCTG TGTCTTATGT ACCCACTGAG CCTGGGGACT ACAACATCAA CATCCTTTTT 
GCTGACACCC ACATTCCTGG ATCCCCATTC AAGGCCCATG TGGCTCCTTG TTTTGATGCA 
TCCAAGGTGA AGTGCTCAGG CCCTGGGCTG GAGCGGGCTA CTGCTGGTGA GGTAGGGCAG 
TTCCAAGTGG ACTGTTCAAG TGCTGGCAGT GCTGAGTTGA CGATTGAGAT CTGCTCTGAG 
GCAGGACTGC CAGCTGAAGT ATACATTCAA GACCATGGTG ATGGCACACA CACCATTACC 
TATATTCCTC TCTGTCCTGG GGCTTACACT GTTACCATCA AGTATGGCGG CCAGCCTGTG 
CCCAACTTCC CCAGCAAGCT ACAGGTGGAA CCTGCTGTAG ATACCTCAGG TGTACAGTGC 
TATGGGCCTG GGATTGAAGG TCAAGGTGTC TTCCGAGAGG CAACCACTGA GTTCAGTGTG 
GATGCCCGGG CTCTTACACA GACTGGAGGG CCACATGTCA AGGCTCGAGT GGCCAACCCC 
TCAGGCAATC TGACAGATAC CTATGTGCAA GACTGTGGGG ATGGCACATA CAAAGTGGAA 
TACACTCCAT ATGAGGAAGG AGTACACTCT GTGGATGTGA CTTATGATGG CAGCCCTGTG 
CCCAGCAGCC CCTTCCAGGT GCCTGTAACA GAGGGCTGTG ACCCCTCCCG GGTGCGTGTC 
CATGGACCAG GCATCCAAAG TGGTACCACC AACAAACCCA ACAAGTTCAC AGTAGAAACC 
AGGGGAGCTG GCACAGGTGG CCTGGGCTTG GCTGTTGAGG GTCCCTCAGA GGCCAAGATG 
TCTTGTATGG ATAATAAAGA TGGCAGCTGC TCAGTAGAAT ACATCCCCTA TGAAGCTGGA 
ACCTATAGCC TTAATGTCAC TTATGGTGGT CACCAAGTGC CAGGTAGTCC CTTCAAGGTC 
CCTGTACATG ATGTGACAGA TGCATCTAAA GTCAAGTGTT CTGGACCTGG CCTAAGCCCA 
GGCATGGTCC GTGCCAACCT CCCTCAGTCC TTTCAGGTGG ACACAAGCAA AGCTGGAGTT 
GCCCCACTGC AGGTCAAAGT GCAGGGGCCC AAAGGCCTGG TGGAGCCAGT GGATGTAGTG 
GACAATGCTG ATGGTACTCA GACTGTCAAC TATGTGCCCA GCCGAGAAGG GTCCTATAGC 
ATTTCTGTGC TGTATGGTGA AGAAGAAGTG CCACGGAGCC CCTTCAAGGT CAAGGTGCTG 
CCTACACATG ATGCCAGTAA GGTGAAGGCC AGTGGACCTG GACTCAACAC CACTGGTGTA 
CCTGCTAGCC TGCCTGTGGA GTTCACCATT GATGCCAAGG ATGCTGGGGA GGGTCTGTTG 
GCTGTCCAGA TTACGGATCC TGAAGGCAAG CCCAAGAAGA CACACATTCA AGATAATCAT 
GATGGCACAT ACACGGTGGC TTATGTGCCA GATGTGCCAG GCCGGTACAC AATCCTCATC 
AAGTATGGTG GTGATGAGAT TCCCTTTTCC CCGTACCGTG TCCGGGCTGT GCCCACTGGG 
GATGCCAGCA AGTGCACAGT CACAGGTGCT GGCATTGGCC CCACCATCCA GATTGGGGAG 
GAGACGGTGA TTACTGTGGA CACAAAAGCA GCAGGCAAAG GCAAAGTGAC TTGTACTGTG 
TGCACACCTG ATGGCTCAGA GGTAGACGTG GACGTGGTGG AGAATGAGGA TGGCACCTTT 
GACATCTTCT ACACAGCTCC CCAACCGGGC AAATATGTCA TCTGTGTGCG CTTCGGTGGC 
GAGCATGTGC CCAACAGCCC CTTCCAAGTT ACAGCTTTGG CTGGGGACCA ACCAACAGTG 
CAGACCCCAT TAAGGTCTCA GCAGCTGGCT CCACAGTATA ACTATCCTCA GGGTAGCCAG 
CAAACCTGGA TTCCAGAGAG GCCCATGGTG GGCGTTAATG GGCTGGATGT GACCAGCCTG 
AGGCCCTTTG ATCTTGTCAT CCCCTTCACT ATCAAGAAGG GGGAGATCAC TGGGGAAGTT 
CGAATGCCCT CAGGCAAGGT GGCCCAGCCT TCCATTACTG ATAACAAAGA TGGCACTGTT 
ACTGTACGTT ACTCACCCAG TGAAGCTGGC CTGCATGAAA TGGACATTCG CTATGACAAT 
ATGCATATCC CAGGAAGCCC TCTGCAGTTC TATGTTGATT ATGTCAACTG TGGCCACATC 
ACTGCTTATG GTCCTGGCCT TACCCATGGA GTGGTCAACA AACCTGCCAC CTTCACTGTC 
AACACCAAGG ATGCAGGAGA GGGGGGCTTG TCTCTGGCCA TTGAGGGTCC ATCTAAAGCA 
GAAATCAGTT GCACTGACAA CCAGGATGGA ACATGCAGTG TCTCTTACCT GCCTGTACTG 
CCTGGTGACT ATAGCATCCT AGTTAAGTAC AATGATCAAC ACATCCCAGG CAGTCCCTTT 
ACTGCCAGAG TAACAGGTGA CGATTCCATG CGTATGTCCC ACCTAAAGGT GGGTTCTGCT 
GCTGATATCC CCATCAATAT CTCAGAAACA GACCTTAGCC TACTCACAGC CACTGTGGTG 
CCACCTTCGG GTCGAGAGGA ACCCTGTCTG CTGAAACGTT TGCGAAATGG CCACGTGGGG 
ATTTCCTTCG TGCCCAAGGA GACAGGGGAG CACCTGGTAC ATGTGAAGAA GAATGGCCAG 
CATGTGGCAA GCAGTCCCAT CCCAGTAGTG ATCAGCCAGT CGGAGATAGG TGATGCCAGC 
CGTGTGAGGG TCTCTGGTCA AGGTCTTCAT GAAGGTCATA CCTTTGAGCC TGCAGAGTTT 
ATTATTGACA CCAGAGATGC AGGCTACGGT GGGCTTAGTC TGTCCATTGA GGGCCCTAGC 
AAAGTAGACA TCAACACAGA GGATCTGGAG GATGGCACAT GCAGGGTCAC CTACTGTCCC 
ACAGAGCCTG GAAACTACAT TATAAACATC AAATTTGCTG ACCAGCATGT GCCTGGCAGT 
CCCTTTTCTG TGAAGGTGAC AGGTGAGGGC CGGGTGAAAG AGAGTATCAC ACGCAGGCGA 
CGTGCCCCTT CTGTGGCCAA TATTGGCAGT CATTGTGACC TCAGCCTGAA GATTCCTGAA 
ATTAGCATCC AAGATATGAC AGCCCAGGTG ACCAGCCCAT CAGGCAAGAC CCATGAGGCA 
GAGATCGTAG AAGGAGAGAA CCATACTTAC TGTATCCGAT TTGTGCCTGC TGAGATGGGA 
ATGCATACAG TCAGTGTCAA GTACAAGGGC CAGCATGTAC CTGGGAGCCC CTTCCAGTTC 
ACTGTGGGGC CTCTGGGGGA AGGGGGTGCT CACAAGGTCC GTGCTGGAGG CCCTGGCCTA 
GAGAGGGCTG AAGTTGGAGT GCCAGCGGAG TTCGGCATTT GGACTAGGGA AGCTGGCGCT 
GGAGGCCTGG CCATTGCTGT TGAAGGCCCC AGCAAGGCTG AGATCTCTTT CGAGGACCGA 
AAGGATGGCT CCTGTGGTGT GGCCTACGTA GTTCAGGAGC CAGGTGACTA TGAGGTCTCA 
GTCAAGTTCA ACGAGGAGCA CATACCTGAT AGCCCCTTCG TGGTGCCTGT GGCTTCTCCG 
TCTGGTGACG CCCGCCGCCT TACTGTTTCT AGTCTTCAGG AGTCAGGGTT AAAGGTCAAC 
CAGCCAGCAT CTTTTGCAGT CAGTCTGAAT GGAGCCAAGG GGGCAATTGA TGCCAAGGTG 
CACAGCCCCT CAGGAGCTCT GGAGGAGTGC TATGTCACAG AGATTGACCA AGATAAGTAT 
GCTGTGCGTT TCATCCCACG AGAGAATGGC ATCTACTTGA TTGATGTCAA GTTCAATGGT 
ACTCACATTC CTGGAAGTCC CTTCAAGATC CGAGTTGGGG AGCCTGGGCA TGGAGGGGAC 
CCAGGCTTAG TGTCCGCCTA TGGAGCAGGC CTGGAAGGTG GTGTCACAGG GAGCCCAGCA 
GAGTTTATTG TGAACACAAG CAATGCAGGA GCTGGTGCCC TTTCGGTTAC CATTGATGGC 
CCCTCCAAGG TGAAGATGGA TTGCCAGGAG TGCCCCGAGG GCTATCGTGT CACCTATACC 
CCCATGGCAC CTGGCAGCTA CCTCATCTCC ATCAAGTATG GTGGCCCCTA TCACATTGGT 
GGAAGTCCCT TTAAAGCCAA GGTCACAGGT CCTCGTCTTG TAAGCAACCA CAGCCTCCAT 
GAGACATCAT CTGTGTTTGT GGACTCTTTG ACTAAAGTTG CCACGGTTCC CCAGCATGCA 
ACCTCAGGCC CAGGTCCTGC TGATGTCAGC AAGGTAGTAG CCAAAGGCCT GGGGCTAAGC 
AAGGCTTATG TAGGCCAGAA GAGCAACTTC ACAGTAGATT GCAGCAAAGC AGGTAACAAC 
ATGCTGCTGG TGGGCGTGCA TGGCCCAAGG ACACCCTGTG AAGAGATCCT GGTGAAACAC 
ATGGGCAGCC GCCTCTATAG TGTCTCTTAC CTGCTCAAGG ACAAAGGGGA GTACACACTG 
GTGGTCAAGT GGGGTGATGA GCATATCCCA GGCAGCCCAT ACCGCATTAT GGTACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245851.1
CDS	242..8161
Translation	MSSSHSRCGQSAAVASPGGSIDSRDAEMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVSKRIANLQTDLSDGLRLIALLEVLSQKKMHRKHNQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEDEEAKKQTPKQRLLGWIQNKLPQLPITNFSRDWQSGRALGALVDSCAPGLCPDWDSWDASKPVNNAREAMQQADDWLGIPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNMVKKRAEFTVETRSAGQGEVLVYVEDPAGHQEEAKVTANNDKNRTFSVWYVPEVTGTHKVTVLFAGQHIAKSPFEVYVDKSQGDASKVTAQGPGLEPSGNIANKTTYFEIFTAGAGMGEVEVVIQDPTGQKGTVEPQLEARGDSTYRCSYQPTMEGVHTVHVTFAGVPIPRSPYTVTVGQACNPAACRAIGRGLQPKGVRVKETADFKVYTKGAGSGELKVTVKGPKGEERVKQKDLGDGVYGFEYYPTIPGTYTVTITWGGQNIGRSPFEVKVGTECGNQKVRAWGPGLEGGIVGKSADFVVEAIGDDVGTLGFSVEGPSQAKIECDDKGDGSCDVRYWPQEAGEYAVHVLCNSEDIRLSPFMADIREAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAKHAGKAPLRVQVQDNEGCSVEATVKDNGNGTYSCSYVPRKPVKHTAMVSWGGVSIPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCTEAGQGDVSIGIKCAPGVVGPTEADIDFDIIRNDNDTFTVKYTPCGAGSYTIMVLFADQATPTSPIRVKVEPSHDASKVKAEGPGLNRTGVELGKPTHFTVNAKTAGKGKLDVQFSGLAKGDAVRDVDIIDHHDNTYTVKYIPVQQGPVGVNVTYGGDHIPKSPFSVGVSPSLDLSKIKVSGLGDKVDVGKDQEFTVKSKGAGGQGKVASKIVSPSGAAVPCKVEPGLGADNSVVRFVPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQGGNAGSPARFTIDTKGAGTGGLGLTVEGPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVAPCFDASKVKCSGPGLERATAGEVGQFQVDCSSAGSAELTIEICSEAGLPAEVYIQDHGDGTHTITYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTGGPHVKARVANPSGNLTDTYVQDCGDGTYKVEYTPYEEGVHSVDVTYDGSPVPSSPFQVPVTEGCDPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKMSCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDVVDNADGTQTVNYVPSREGSYSISVLYGEEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVPDVPGRYTILIKYGGDEIPFSPYRVRAVPTGDASKCTVTGAGIGPTIQIGEETVITVDTKAAGKGKVTCTVCTPDGSEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPTVQTPLRSQQLAPQYNYPQGSQQTWIPERPMVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPSITDNKDGTVTVRYSPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHITAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNDQHIPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANIGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGMHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEVGVPAEFGIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGIYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGSPAEFIVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKVATVPQHATSGPGPADVSKVVAKGLGLSKAYVGQKSNFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHMGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRIMVP

Target ORF information:

RefSeq Version	XM_011247549.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus filamin, alpha (Flna), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247549.3

ORF Insert Sequence:

ATGAGTAGCT CTCACTCCCG CTGTGGCCAG AGTGCGGCGG TTGCGTCTCC GGGAGGCAGT 
ATCGATTCAC GGGACGCGGA GATGCCGGCT ACCGAAAAAG ACCTAGCAGA AGATGCACCG 
TGGAAGAAAA TTCAGCAGAA CACATTCACC CGCTGGTGCA ATGAGCACCT TAAGTGCGTA 
AGCAAGCGCA TCGCCAATCT GCAGACGGAC CTGAGCGATG GGTTGCGGCT CATCGCGCTG 
CTCGAGGTAC TCAGCCAGAA GAAAATGCAC CGCAAGCACA ACCAAAGACC CACTTTCCGC 
CAGATGCAGC TCGAAAATGT GTCGGTGGCG CTTGAATTCC TGGACCGTGA GAGCATCAAG 
CTCGTGTCCA TAGACAGCAA GGCTATTGTG GATGGAAATC TGAAGCTGAT CTTAGGCCTC 
ATCTGGACCC TGATCCTGCA CTATTCCATC TCAATGCCCA TGTGGGATGA GGAAGAGGAT 
GAGGAGGCCA AGAAGCAAAC ACCCAAGCAG AGGCTTCTAG GCTGGATTCA GAACAAGCTA 
CCACAGCTTC CCATTACCAA CTTCAGTCGA GACTGGCAGA GTGGCCGGGC CCTGGGTGCT 
CTTGTTGATA GCTGTGCCCC AGGCCTATGT CCTGACTGGG ACTCCTGGGA TGCTAGTAAG 
CCTGTGAACA ATGCACGGGA AGCCATGCAG CAGGCTGATG ACTGGCTAGG CATTCCTCAG 
GTGATTACCC CAGAAGAAAT TGTGGACCCC AATGTAGATG AGCATTCTGT TATGACCTAC 
CTGTCTCAGT TTCCCAAGGC CAAGCTGAAG CCAGGGGCTC CTCTTCGGCC CAAACTGAAC 
CCGAAGAAAG CCCGAGCCTA TGGGCCAGGC ATCGAGCCTA CAGGCAATAT GGTGAAGAAG 
AGAGCAGAAT TCACTGTGGA GACCCGAAGT GCTGGACAGG GAGAAGTGCT TGTATATGTG 
GAGGACCCAG CTGGACACCA GGAAGAGGCA AAAGTGACTG CCAATAATGA CAAGAACCGT 
ACTTTCTCTG TCTGGTATGT CCCTGAAGTG ACAGGGACTC ATAAGGTGAC TGTGCTCTTT 
GCTGGCCAAC ATATTGCCAA GAGCCCCTTT GAGGTGTATG TGGACAAGTC ACAGGGTGAT 
GCCAGCAAAG TGACTGCCCA GGGCCCTGGT CTGGAGCCCA GTGGCAATAT TGCCAACAAG 
ACTACCTACT TTGAGATCTT CACTGCAGGA GCTGGCATGG GTGAGGTGGA AGTTGTCATC 
CAGGACCCTA CAGGACAGAA AGGCACAGTG GAACCTCAGC TGGAGGCCAG GGGTGACAGC 
ACCTATCGCT GTAGCTATCA GCCCACCATG GAGGGTGTCC ATACAGTACA TGTCACCTTC 
GCCGGTGTTC CCATCCCTCG TAGCCCCTAC ACTGTCACTG TTGGCCAAGC CTGTAACCCA 
GCTGCCTGCC GGGCTATTGG TAGAGGCCTT CAGCCCAAGG GTGTTCGAGT GAAGGAAACA 
GCCGACTTCA AGGTGTACAC AAAGGGCGCT GGCAGTGGGG AGCTAAAGGT CACTGTAAAG 
GGTCCCAAGG GTGAGGAGCG TGTAAAGCAG AAGGACTTAG GGGATGGTGT GTATGGCTTT 
GAATATTACC CTACAATCCC TGGCACATAC ACTGTCACCA TCACATGGGG TGGCCAGAAC 
ATTGGTCGAA GTCCGTTTGA GGTGAAGGTA GGCACTGAGT GTGGCAATCA GAAAGTTCGG 
GCATGGGGTC CTGGCCTGGA AGGAGGCATT GTTGGCAAGT CAGCAGACTT CGTAGTAGAG 
GCCATTGGTG ATGATGTGGG CACCTTGGGT TTCTCTGTGG AAGGTCCATC ACAGGCAAAG 
ATTGAATGTG ACGACAAGGG TGATGGCTCC TGTGATGTGC GCTATTGGCC CCAGGAGGCT 
GGCGAGTATG CTGTTCATGT GCTGTGTAAC AGTGAGGATA TCCGTCTCAG TCCTTTCATG 
GCTGACATCC GTGAGGCACC CCAGGATTTT CACCCAGACA GGGTGAAGGC ACGTGGGCCT 
GGATTGGAGA AGACTGGTGT GGCTGTCAAC AAGCCAGCAG AGTTCACAGT TGATGCCAAG 
CATGCTGGGA AGGCTCCTCT TCGAGTTCAA GTTCAGGACA ATGAGGGCTG CTCTGTGGAA 
GCGACAGTCA AGGACAATGG CAATGGTACT TACAGCTGTT CTTATGTGCC CAGAAAGCCA 
GTGAAGCACA CAGCCATGGT TTCTTGGGGA GGTGTCAGCA TCCCCAACAG TCCTTTCCGG 
GTGAATGTGG GAGCTGGCAG CCATCCAAAC AAAGTCAAGG TGTATGGTCC AGGAGTGGCC 
AAGACTGGGC TCAAGGCCCA TGAACCTACC TACTTTACTG TGGATTGTAC TGAAGCTGGC 
CAGGGAGATG TCAGCATTGG TATCAAGTGT GCCCCTGGAG TAGTGGGCCC CACTGAGGCT 
GATATTGACT TTGATATCAT CCGCAATGAC AATGACACCT TCACTGTAAA ATACACACCC 
TGTGGGGCTG GCAGCTATAC CATCATGGTC CTTTTTGCTG ACCAGGCCAC ACCCACCAGC 
CCCATCAGAG TCAAAGTGGA GCCTTCTCAT GATGCCAGCA AGGTGAAGGC TGAGGGTCCT 
GGCCTAAATC GCACTGGTGT TGAACTTGGC AAACCCACCC ATTTCACAGT CAATGCTAAA 
ACTGCTGGGA AAGGCAAGCT GGATGTCCAA TTCTCAGGAC TGGCTAAGGG AGATGCAGTA 
CGGGATGTGG ACATCATTGA CCACCATGAT AATACCTACA CAGTCAAGTA CATTCCTGTG 
CAGCAGGGCC CAGTAGGTGT CAATGTCACT TATGGAGGAG ATCACATCCC CAAGAGTCCA 
TTTTCAGTGG GAGTATCTCC AAGCCTGGAT CTCAGCAAAA TCAAGGTGTC TGGCCTTGGT 
GACAAAGTGG ACGTTGGCAA AGATCAAGAG TTCACAGTAA AGTCAAAGGG TGCAGGTGGT 
CAAGGCAAAG TAGCATCCAA GATTGTGAGT CCCTCAGGTG CAGCGGTACC CTGCAAGGTA 
GAGCCAGGCC TGGGAGCTGA CAACAGCGTG GTACGTTTTG TGCCCCGTGA AGAGGGGCCC 
TATGAGGTGG AAGTGACCTA TGATGGTGTG CCTGTACCTG GCAGTCCCTT TCCACTAGAA 
GCTGTGGCCC CCACCAAACC CAGCAAGGTG AAGGCGTTTG GACCAGGGCT ACAGGGGGGC 
AATGCAGGCT CCCCTGCCCG CTTCACCATT GATACAAAGG GTGCTGGCAC TGGTGGCCTG 
GGCCTGACAG TGGAAGGCCC CTGTGAAGCA CAGCTTGAGT GCCTAGACAA CGGGGATGGT 
ACATGCTCTG TGTCTTATGT ACCCACTGAG CCTGGGGACT ACAACATCAA CATCCTTTTT 
GCTGACACCC ACATTCCTGG ATCCCCATTC AAGGCCCATG TGGCTCCTTG TTTTGATGCA 
TCCAAGGTGA AGTGCTCAGG CCCTGGGCTG GAGCGGGCTA CTGCTGGTGA GGTAGGGCAG 
TTCCAAGTGG ACTGTTCAAG TGCTGGCAGT GCTGAGTTGA CGATTGAGAT CTGCTCTGAG 
GCAGGACTGC CAGCTGAAGT ATACATTCAA GACCATGGTG ATGGCACACA CACCATTACC 
TATATTCCTC TCTGTCCTGG GGCTTACACT GTTACCATCA AGTATGGCGG CCAGCCTGTG 
CCCAACTTCC CCAGCAAGCT ACAGGTGGAA CCTGCTGTAG ATACCTCAGG TGTACAGTGC 
TATGGGCCTG GGATTGAAGG TCAAGGTGTC TTCCGAGAGG CAACCACTGA GTTCAGTGTG 
GATGCCCGGG CTCTTACACA GACTGGAGGG CCACATGTCA AGGCTCGAGT GGCCAACCCC 
TCAGGCAATC TGACAGATAC CTATGTGCAA GACTGTGGGG ATGGCACATA CAAAGTGGAA 
TACACTCCAT ATGAGGAAGG AGTACACTCT GTGGATGTGA CTTATGATGG CAGCCCTGTG 
CCCAGCAGCC CCTTCCAGGT GCCTGTAACA GAGGGCTGTG ACCCCTCCCG GGTGCGTGTC 
CATGGACCAG GCATCCAAAG TGGTACCACC AACAAACCCA ACAAGTTCAC AGTAGAAACC 
AGGGGAGCTG GCACAGGTGG CCTGGGCTTG GCTGTTGAGG GTCCCTCAGA GGCCAAGATG 
TCTTGTATGG ATAATAAAGA TGGCAGCTGC TCAGTAGAAT ACATCCCCTA TGAAGCTGGA 
ACCTATAGCC TTAATGTCAC TTATGGTGGT CACCAAGTGC CAGGTAGTCC CTTCAAGGTC 
CCTGTACATG ATGTGACAGA TGCATCTAAA GTCAAGTGTT CTGGACCTGG CCTAAGCCCA 
GGCATGGTCC GTGCCAACCT CCCTCAGTCC TTTCAGGTGG ACACAAGCAA AGCTGGAGTT 
GCCCCACTGC AGGTCAAAGT GCAGGGGCCC AAAGGCCTGG TGGAGCCAGT GGATGTAGTG 
GACAATGCTG ATGGTACTCA GACTGTCAAC TATGTGCCCA GCCGAGAAGG GTCCTATAGC 
ATTTCTGTGC TGTATGGTGA AGAAGAAGTG CCACGGAGCC CCTTCAAGGT CAAGGTGCTG 
CCTACACATG ATGCCAGTAA GGTGAAGGCC AGTGGACCTG GACTCAACAC CACTGGTGTA 
CCTGCTAGCC TGCCTGTGGA GTTCACCATT GATGCCAAGG ATGCTGGGGA GGGTCTGTTG 
GCTGTCCAGA TTACGGATCC TGAAGGCAAG CCCAAGAAGA CACACATTCA AGATAATCAT 
GATGGCACAT ACACGGTGGC TTATGTGCCA GATGTGCCAG GCCGGTACAC AATCCTCATC 
AAGTATGGTG GTGATGAGAT TCCCTTTTCC CCGTACCGTG TCCGGGCTGT GCCCACTGGG 
GATGCCAGCA AGTGCACAGT CACAGGTGCT GGCATTGGCC CCACCATCCA GATTGGGGAG 
GAGACGGTGA TTACTGTGGA CACAAAAGCA GCAGGCAAAG GCAAAGTGAC TTGTACTGTG 
TGCACACCTG ATGGCTCAGA GGTAGACGTG GACGTGGTGG AGAATGAGGA TGGCACCTTT 
GACATCTTCT ACACAGCTCC CCAACCGGGC AAATATGTCA TCTGTGTGCG CTTCGGTGGC 
GAGCATGTGC CCAACAGCCC CTTCCAAGTT ACAGCTTTGG CTGGGGACCA ACCAACAGTG 
CAGACCCCAT TAAGGTCTCA GCAGCTGGCT CCACAGTATA ACTATCCTCA GGGTAGCCAG 
CAAACCTGGA TTCCAGAGAG GCCCATGGTG GGCGTTAATG GGCTGGATGT GACCAGCCTG 
AGGCCCTTTG ATCTTGTCAT CCCCTTCACT ATCAAGAAGG GGGAGATCAC TGGGGAAGTT 
CGAATGCCCT CAGGCAAGGT GGCCCAGCCT TCCATTACTG ATAACAAAGA TGGCACTGTT 
ACTGTACGTT ACTCACCCAG TGAAGCTGGC CTGCATGAAA TGGACATTCG CTATGACAAT 
ATGCATATCC CAGGAAGCCC TCTGCAGTTC TATGTTGATT ATGTCAACTG TGGCCACATC 
ACTGCTTATG GTCCTGGCCT TACCCATGGA GTGGTCAACA AACCTGCCAC CTTCACTGTC 
AACACCAAGG ATGCAGGAGA GGGGGGCTTG TCTCTGGCCA TTGAGGGTCC ATCTAAAGCA 
GAAATCAGTT GCACTGACAA CCAGGATGGA ACATGCAGTG TCTCTTACCT GCCTGTACTG 
CCTGGTGACT ATAGCATCCT AGTTAAGTAC AATGATCAAC ACATCCCAGG CAGTCCCTTT 
ACTGCCAGAG TAACAGGTGA CGATTCCATG CGTATGTCCC ACCTAAAGGT GGGTTCTGCT 
GCTGATATCC CCATCAATAT CTCAGAAACA GACCTTAGCC TACTCACAGC CACTGTGGTG 
CCACCTTCGG GTCGAGAGGA ACCCTGTCTG CTGAAACGTT TGCGAAATGG CCACGTGGGG 
ATTTCCTTCG TGCCCAAGGA GACAGGGGAG CACCTGGTAC ATGTGAAGAA GAATGGCCAG 
CATGTGGCAA GCAGTCCCAT CCCAGTAGTG ATCAGCCAGT CGGAGATAGG TGATGCCAGC 
CGTGTGAGGG TCTCTGGTCA AGGTCTTCAT GAAGGTCATA CCTTTGAGCC TGCAGAGTTT 
ATTATTGACA CCAGAGATGC AGGCTACGGT GGGCTTAGTC TGTCCATTGA GGGCCCTAGC 
AAAGTAGACA TCAACACAGA GGATCTGGAG GATGGCACAT GCAGGGTCAC CTACTGTCCC 
ACAGAGCCTG GAAACTACAT TATAAACATC AAATTTGCTG ACCAGCATGT GCCTGGCAGT 
CCCTTTTCTG TGAAGGTGAC AGGTGAGGGC CGGGTGAAAG AGAGTATCAC ACGCAGGCGA 
CGTGCCCCTT CTGTGGCCAA TATTGGCAGT CATTGTGACC TCAGCCTGAA GATTCCTGAA 
ATTAGCATCC AAGATATGAC AGCCCAGGTG ACCAGCCCAT CAGGCAAGAC CCATGAGGCA 
GAGATCGTAG AAGGAGAGAA CCATACTTAC TGTATCCGAT TTGTGCCTGC TGAGATGGGA 
ATGCATACAG TCAGTGTCAA GTACAAGGGC CAGCATGTAC CTGGGAGCCC CTTCCAGTTC 
ACTGTGGGGC CTCTGGGGGA AGGGGGTGCT CACAAGGTCC GTGCTGGAGG CCCTGGCCTA 
GAGAGGGCTG AAGTTGGAGT GCCAGCGGAG TTCGGCATTT GGACTAGGGA AGCTGGCGCT 
GGAGGCCTGG CCATTGCTGT TGAAGGCCCC AGCAAGGCTG AGATCTCTTT CGAGGACCGA 
AAGGATGGCT CCTGTGGTGT GGCCTACGTA GTTCAGGAGC CAGGTGACTA TGAGGTCTCA 
GTCAAGTTCA ACGAGGAGCA CATACCTGAT AGCCCCTTCG TGGTGCCTGT GGCTTCTCCG 
TCTGGTGACG CCCGCCGCCT TACTGTTTCT AGTCTTCAGG AGTCAGGGTT AAAGGTCAAC 
CAGCCAGCAT CTTTTGCAGT CAGTCTGAAT GGAGCCAAGG GGGCAATTGA TGCCAAGGTG 
CACAGCCCCT CAGGAGCTCT GGAGGAGTGC TATGTCACAG AGATTGACCA AGATAAGTAT 
GCTGTGCGTT TCATCCCACG AGAGAATGGC ATCTACTTGA TTGATGTCAA GTTCAATGGT 
ACTCACATTC CTGGAAGTCC CTTCAAGATC CGAGTTGGGG AGCCTGGGCA TGGAGGGGAC 
CCAGGCTTAG TGTCCGCCTA TGGAGCAGGC CTGGAAGGTG GTGTCACAGG GAGCCCAGCA 
GAGTTTATTG TGAACACAAG CAATGCAGGA GCTGGTGCCC TTTCGGTTAC CATTGATGGC 
CCCTCCAAGG TGAAGATGGA TTGCCAGGAG TGCCCCGAGG GCTATCGTGT CACCTATACC 
CCCATGGCAC CTGGCAGCTA CCTCATCTCC ATCAAGTATG GTGGCCCCTA TCACATTGGT 
GGAAGTCCCT TTAAAGCCAA GGTCACAGGT CCTCGTCTTG TAAGCAACCA CAGCCTCCAT 
GAGACATCAT CTGTGTTTGT GGACTCTTTG ACTAAAGTTG CCACGGTTCC CCAGCATGCA 
ACCTCAGGCC CAGGTCCTGC TGATGTCAGC AAGGTAGTAG CCAAAGGCCT GGGGCTAAGC 
AAGGCTTATG TAGGCCAGAA GAGCAACTTC ACAGTAGATT GCAGCAAAGC AGGTAACAAC 
ATGCTGCTGG TGGGCGTGCA TGGCCCAAGG ACACCCTGTG AAGAGATCCT GGTGAAACAC 
ATGGGCAGCC GCCTCTATAG TGTCTCTTAC CTGCTCAAGG ACAAAGGGGA GTACACACTG 
GTGGTCAAGT GGGGTGATGA GCATATCCCA GGCAGCCCAT ACCGCATTAT GGTACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Flna ( XM_006527911.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527974.1 Mus musculus filamin, alpha (Flna), transcript variant X1, mRNA.

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CloneID	OMu56214
Clone ID Related Accession (Same CDS sequence)	XM_006527911.4 , XM_006527911.3
Accession Version	XM_006527911.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	filamin-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527911.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCT CTCACTCCCG CTGTGGCCAG AGTGCGGCGG TTGCGTCTCC GGGAGGCAGT 
ATCGATTCAC GGGACGCGGA GATGCCGGCT ACCGAAAAAG ACCTAGCAGA AGATGCACCG 
TGGAAGAAAA TTCAGCAGAA CACATTCACC CGCTGGTGCA ATGAGCACCT TAAGTGCGTA 
AGCAAGCGCA TCGCCAATCT GCAGACGGAC CTGAGCGATG GGTTGCGGCT CATCGCGCTG 
CTCGAGGTAC TCAGCCAGAA GAAAATGCAC CGCAAGCACA ACCAAAGACC CACTTTCCGC 
CAGATGCAGC TCGAAAATGT GTCGGTGGCG CTTGAATTCC TGGACCGTGA GAGCATCAAG 
CTCGTGTCCA TAGACAGCAA GGCTATTGTG GATGGAAATC TGAAGCTGAT CTTAGGCCTC 
ATCTGGACCC TGATCCTGCA CTATTCCATC TCAATGCCCA TGTGGGATGA GGAAGAGGAT 
GAGGAGGCCA AGAAGCAAAC ACCCAAGCAG AGGCTTCTAG GCTGGATTCA GAACAAGCTA 
CCACAGCTTC CCATTACCAA CTTCAGTCGA GACTGGCAGA GTGGCCGGGC CCTGGGTGCT 
CTTGTTGATA GCTGTGCCCC AGGCCTATGT CCTGACTGGG ACTCCTGGGA TGCTAGTAAG 
CCTGTGAACA ATGCACGGGA AGCCATGCAG CAGGCTGATG ACTGGCTAGG CATTCCTCAG 
GTGATTACCC CAGAAGAAAT TGTGGACCCC AATGTAGATG AGCATTCTGT TATGACCTAC 
CTGTCTCAGT TTCCCAAGGC CAAGCTGAAG CCAGGGGCTC CTCTTCGGCC CAAACTGAAC 
CCGAAGAAAG CCCGAGCCTA TGGGCCAGGC ATCGAGCCTA CAGGCAATAT GGTGAAGAAG 
AGAGCAGAAT TCACTGTGGA GACCCGAAGT GCTGGACAGG GAGAAGTGCT TGTATATGTG 
GAGGACCCAG CTGGACACCA GGAAGAGGCA AAAGTGACTG CCAATAATGA CAAGAACCGT 
ACTTTCTCTG TCTGGTATGT CCCTGAAGTG ACAGGGACTC ATAAGGTGAC TGTGCTCTTT 
GCTGGCCAAC ATATTGCCAA GAGCCCCTTT GAGGTGTATG TGGACAAGTC ACAGGGTGAT 
GCCAGCAAAG TGACTGCCCA GGGCCCTGGT CTGGAGCCCA GTGGCAATAT TGCCAACAAG 
ACTACCTACT TTGAGATCTT CACTGCAGGA GCTGGCATGG GTGAGGTGGA AGTTGTCATC 
CAGGACCCTA CAGGACAGAA AGGCACAGTG GAACCTCAGC TGGAGGCCAG GGGTGACAGC 
ACCTATCGCT GTAGCTATCA GCCCACCATG GAGGGTGTCC ATACAGTACA TGTCACCTTC 
GCCGGTGTTC CCATCCCTCG TAGCCCCTAC ACTGTCACTG TTGGCCAAGC CTGTAACCCA 
GCTGCCTGCC GGGCTATTGG TAGAGGCCTT CAGCCCAAGG GTGTTCGAGT GAAGGAAACA 
GCCGACTTCA AGGTGTACAC AAAGGGCGCT GGCAGTGGGG AGCTAAAGGT CACTGTAAAG 
GGTCCCAAGG GTGAGGAGCG TGTAAAGCAG AAGGACTTAG GGGATGGTGT GTATGGCTTT 
GAATATTACC CTACAATCCC TGGCACATAC ACTGTCACCA TCACATGGGG TGGCCAGAAC 
ATTGGTCGAA GTCCGTTTGA GGTGAAGGTA GGCACTGAGT GTGGCAATCA GAAAGTTCGG 
GCATGGGGTC CTGGCCTGGA AGGAGGCATT GTTGGCAAGT CAGCAGACTT CGTAGTAGAG 
GCCATTGGTG ATGATGTGGG CACCTTGGGT TTCTCTGTGG AAGGTCCATC ACAGGCAAAG 
ATTGAATGTG ACGACAAGGG TGATGGCTCC TGTGATGTGC GCTATTGGCC CCAGGAGGCT 
GGCGAGTATG CTGTTCATGT GCTGTGTAAC AGTGAGGATA TCCGTCTCAG TCCTTTCATG 
GCTGACATCC GTGAGGCACC CCAGGATTTT CACCCAGACA GGGTGAAGGC ACGTGGGCCT 
GGATTGGAGA AGACTGGTGT GGCTGTCAAC AAGCCAGCAG AGTTCACAGT TGATGCCAAG 
CATGCTGGGA AGGCTCCTCT TCGAGTTCAA GTTCAGGACA ATGAGGGCTG CTCTGTGGAA 
GCGACAGTCA AGGACAATGG CAATGGTACT TACAGCTGTT CTTATGTGCC CAGAAAGCCA 
GTGAAGCACA CAGCCATGGT TTCTTGGGGA GGTGTCAGCA TCCCCAACAG TCCTTTCCGG 
GTGAATGTGG GAGCTGGCAG CCATCCAAAC AAAGTCAAGG TGTATGGTCC AGGAGTGGCC 
AAGACTGGGC TCAAGGCCCA TGAACCTACC TACTTTACTG TGGATTGTAC TGAAGCTGGC 
CAGGGAGATG TCAGCATTGG TATCAAGTGT GCCCCTGGAG TAGTGGGCCC CACTGAGGCT 
GATATTGACT TTGATATCAT CCGCAATGAC AATGACACCT TCACTGTAAA ATACACACCC 
TGTGGGGCTG GCAGCTATAC CATCATGGTC CTTTTTGCTG ACCAGGCCAC ACCCACCAGC 
CCCATCAGAG TCAAAGTGGA GCCTTCTCAT GATGCCAGCA AGGTGAAGGC TGAGGGTCCT 
GGCCTAAATC GCACTGGTGT TGAACTTGGC AAACCCACCC ATTTCACAGT CAATGCTAAA 
ACTGCTGGGA AAGGCAAGCT GGATGTCCAA TTCTCAGGAC TGGCTAAGGG AGATGCAGTA 
CGGGATGTGG ACATCATTGA CCACCATGAT AATACCTACA CAGTCAAGTA CATTCCTGTG 
CAGCAGGGCC CAGTAGGTGT CAATGTCACT TATGGAGGAG ATCACATCCC CAAGAGTCCA 
TTTTCAGTGG GAGTATCTCC AAGCCTGGAT CTCAGCAAAA TCAAGGTGTC TGGCCTTGGT 
GACAAAGTGG ACGTTGGCAA AGATCAAGAG TTCACAGTAA AGTCAAAGGG TGCAGGTGGT 
CAAGGCAAAG TAGCATCCAA GATTGTGAGT CCCTCAGGTG CAGCGGTACC CTGCAAGGTA 
GAGCCAGGCC TGGGAGCTGA CAACAGCGTG GTACGTTTTG TGCCCCGTGA AGAGGGGCCC 
TATGAGGTGG AAGTGACCTA TGATGGTGTG CCTGTACCTG GCAGTCCCTT TCCACTAGAA 
GCTGTGGCCC CCACCAAACC CAGCAAGGTG AAGGCGTTTG GACCAGGGCT ACAGGGGGGC 
AATGCAGGCT CCCCTGCCCG CTTCACCATT GATACAAAGG GTGCTGGCAC TGGTGGCCTG 
GGCCTGACAG TGGAAGGCCC CTGTGAAGCA CAGCTTGAGT GCCTAGACAA CGGGGATGGT 
ACATGCTCTG TGTCTTATGT ACCCACTGAG CCTGGGGACT ACAACATCAA CATCCTTTTT 
GCTGACACCC ACATTCCTGG ATCCCCATTC AAGGCCCATG TGGCTCCTTG TTTTGATGCA 
TCCAAGGTGA AGTGCTCAGG CCCTGGGCTG GAGCGGGCTA CTGCTGGTGA GGTAGGGCAG 
TTCCAAGTGG ACTGTTCAAG TGCTGGCAGT GCTGAGTTGA CGATTGAGAT CTGCTCTGAG 
GCAGGACTGC CAGCTGAAGT ATACATTCAA GACCATGGTG ATGGCACACA CACCATTACC 
TATATTCCTC TCTGTCCTGG GGCTTACACT GTTACCATCA AGTATGGCGG CCAGCCTGTG 
CCCAACTTCC CCAGCAAGCT ACAGGTGGAA CCTGCTGTAG ATACCTCAGG TGTACAGTGC 
TATGGGCCTG GGATTGAAGG TCAAGGTGTC TTCCGAGAGG CAACCACTGA GTTCAGTGTG 
GATGCCCGGG CTCTTACACA GACTGGAGGG CCACATGTCA AGGCTCGAGT GGCCAACCCC 
TCAGGCAATC TGACAGATAC CTATGTGCAA GACTGTGGGG ATGGCACATA CAAAGTGGAA 
TACACTCCAT ATGAGGAAGG AGTACACTCT GTGGATGTGA CTTATGATGG CAGCCCTGTG 
CCCAGCAGCC CCTTCCAGGT GCCTGTAACA GAGGGCTGTG ACCCCTCCCG GGTGCGTGTC 
CATGGACCAG GCATCCAAAG TGGTACCACC AACAAACCCA ACAAGTTCAC AGTAGAAACC 
AGGGGAGCTG GCACAGGTGG CCTGGGCTTG GCTGTTGAGG GTCCCTCAGA GGCCAAGATG 
TCTTGTATGG ATAATAAAGA TGGCAGCTGC TCAGTAGAAT ACATCCCCTA TGAAGCTGGA 
ACCTATAGCC TTAATGTCAC TTATGGTGGT CACCAAGTGC CAGGTAGTCC CTTCAAGGTC 
CCTGTACATG ATGTGACAGA TGCATCTAAA GTCAAGTGTT CTGGACCTGG CCTAAGCCCA 
GGCATGGTCC GTGCCAACCT CCCTCAGTCC TTTCAGGTGG ACACAAGCAA AGCTGGAGTT 
GCCCCACTGC AGGTCAAAGT GCAGGGGCCC AAAGGCCTGG TGGAGCCAGT GGATGTAGTG 
GACAATGCTG ATGGTACTCA GACTGTCAAC TATGTGCCCA GCCGAGAAGG GTCCTATAGC 
ATTTCTGTGC TGTATGGTGA AGAAGAAGTG CCACGGAGCC CCTTCAAGGT CAAGGTGCTG 
CCTACACATG ATGCCAGTAA GGTGAAGGCC AGTGGACCTG GACTCAACAC CACTGGTGTA 
CCTGCTAGCC TGCCTGTGGA GTTCACCATT GATGCCAAGG ATGCTGGGGA GGGTCTGTTG 
GCTGTCCAGA TTACGGATCC TGAAGGCAAG CCCAAGAAGA CACACATTCA AGATAATCAT 
GATGGCACAT ACACGGTGGC TTATGTGCCA GATGTGCCAG GCCGGTACAC AATCCTCATC 
AAGTATGGTG GTGATGAGAT TCCCTTTTCC CCGTACCGTG TCCGGGCTGT GCCCACTGGG 
GATGCCAGCA AGTGCACAGT CACAGTGTCA ATCGGAGGTC ACGGGCTAGG TGCTGGCATT 
GGCCCCACCA TCCAGATTGG GGAGGAGACG GTGATTACTG TGGACACAAA AGCAGCAGGC 
AAAGGCAAAG TGACTTGTAC TGTGTGCACA CCTGATGGCT CAGAGGTAGA CGTGGACGTG 
GTGGAGAATG AGGATGGCAC CTTTGACATC TTCTACACAG CTCCCCAACC GGGCAAATAT 
GTCATCTGTG TGCGCTTCGG TGGCGAGCAT GTGCCCAACA GCCCCTTCCA AGTTACAGCT 
TTGGCTGGGG ACCAACCAAC AGTGCAGACC CCATTAAGGT CTCAGCAGCT GGCTCCACAG 
TATAACTATC CTCAGGGTAG CCAGCAAACC TGGATTCCAG AGAGGCCCAT GGTGGGCGTT 
AATGGGCTGG ATGTGACCAG CCTGAGGCCC TTTGATCTTG TCATCCCCTT CACTATCAAG 
AAGGGGGAGA TCACTGGGGA AGTTCGAATG CCCTCAGGCA AGGTGGCCCA GCCTTCCATT 
ACTGATAACA AAGATGGCAC TGTTACTGTA CGTTACTCAC CCAGTGAAGC TGGCCTGCAT 
GAAATGGACA TTCGCTATGA CAATATGCAT ATCCCAGGAA GCCCTCTGCA GTTCTATGTT 
GATTATGTCA ACTGTGGCCA CATCACTGCT TATGGTCCTG GCCTTACCCA TGGAGTGGTC 
AACAAACCTG CCACCTTCAC TGTCAACACC AAGGATGCAG GAGAGGGGGG CTTGTCTCTG 
GCCATTGAGG GTCCATCTAA AGCAGAAATC AGTTGCACTG ACAACCAGGA TGGAACATGC 
AGTGTCTCTT ACCTGCCTGT ACTGCCTGGT GACTATAGCA TCCTAGTTAA GTACAATGAT 
CAACACATCC CAGGCAGTCC CTTTACTGCC AGAGTAACAG GTGACGATTC CATGCGTATG 
TCCCACCTAA AGGTGGGTTC TGCTGCTGAT ATCCCCATCA ATATCTCAGA AACAGACCTT 
AGCCTACTCA CAGCCACTGT GGTGCCACCT TCGGGTCGAG AGGAACCCTG TCTGCTGAAA 
CGTTTGCGAA ATGGCCACGT GGGGATTTCC TTCGTGCCCA AGGAGACAGG GGAGCACCTG 
GTACATGTGA AGAAGAATGG CCAGCATGTG GCAAGCAGTC CCATCCCAGT AGTGATCAGC 
CAGTCGGAGA TAGGTGATGC CAGCCGTGTG AGGGTCTCTG GTCAAGGTCT TCATGAAGGT 
CATACCTTTG AGCCTGCAGA GTTTATTATT GACACCAGAG ATGCAGGCTA CGGTGGGCTT 
AGTCTGTCCA TTGAGGGCCC TAGCAAAGTA GACATCAACA CAGAGGATCT GGAGGATGGC 
ACATGCAGGG TCACCTACTG TCCCACAGAG CCTGGAAACT ACATTATAAA CATCAAATTT 
GCTGACCAGC ATGTGCCTGG CAGTCCCTTT TCTGTGAAGG TGACAGGTGA GGGCCGGGTG 
AAAGAGAGTA TCACACGCAG GCGACGTGCC CCTTCTGTGG CCAATATTGG CAGTCATTGT 
GACCTCAGCC TGAAGATTCC TGAAATTAGC ATCCAAGATA TGACAGCCCA GGTGACCAGC 
CCATCAGGCA AGACCCATGA GGCAGAGATC GTAGAAGGAG AGAACCATAC TTACTGTATC 
CGATTTGTGC CTGCTGAGAT GGGAATGCAT ACAGTCAGTG TCAAGTACAA GGGCCAGCAT 
GTACCTGGGA GCCCCTTCCA GTTCACTGTG GGGCCTCTGG GGGAAGGGGG TGCTCACAAG 
GTCCGTGCTG GAGGCCCTGG CCTAGAGAGG GCTGAAGTTG GAGTGCCAGC GGAGTTCGGC 
ATTTGGACTA GGGAAGCTGG CGCTGGAGGC CTGGCCATTG CTGTTGAAGG CCCCAGCAAG 
GCTGAGATCT CTTTCGAGGA CCGAAAGGAT GGCTCCTGTG GTGTGGCCTA CGTAGTTCAG 
GAGCCAGGTG ACTATGAGGT CTCAGTCAAG TTCAACGAGG AGCACATACC TGATAGCCCC 
TTCGTGGTGC CTGTGGCTTC TCCGTCTGGT GACGCCCGCC GCCTTACTGT TTCTAGTCTT 
CAGGAGTCAG GGTTAAAGGT CAACCAGCCA GCATCTTTTG CAGTCAGTCT GAATGGAGCC 
AAGGGGGCAA TTGATGCCAA GGTGCACAGC CCCTCAGGAG CTCTGGAGGA GTGCTATGTC 
ACAGAGATTG ACCAAGATAA GTATGCTGTG CGTTTCATCC CACGAGAGAA TGGCATCTAC 
TTGATTGATG TCAAGTTCAA TGGTACTCAC ATTCCTGGAA GTCCCTTCAA GATCCGAGTT 
GGGGAGCCTG GGCATGGAGG GGACCCAGGC TTAGTGTCCG CCTATGGAGC AGGCCTGGAA 
GGTGGTGTCA CAGGGAGCCC AGCAGAGTTT ATTGTGAACA CAAGCAATGC AGGAGCTGGT 
GCCCTTTCGG TTACCATTGA TGGCCCCTCC AAGGTGAAGA TGGATTGCCA GGAGTGCCCC 
GAGGGCTATC GTGTCACCTA TACCCCCATG GCACCTGGCA GCTACCTCAT CTCCATCAAG 
TATGGTGGCC CCTATCACAT TGGTGGAAGT CCCTTTAAAG CCAAGGTCAC AGGTCCTCGT 
CTTGTAAGCA ACCACAGCCT CCATGAGACA TCATCTGTGT TTGTGGACTC TTTGACTAAA 
GTTGCCACGG TTCCCCAGCA TGCAACCTCA GGCCCAGGTC CTGCTGATGT CAGCAAGGTA 
GTAGCCAAAG GCCTGGGGCT AAGCAAGGCT TATGTAGGCC AGAAGAGCAA CTTCACAGTA 
GATTGCAGCA AAGCAGGTAA CAACATGCTG CTGGTGGGCG TGCATGGCCC AAGGACACCC 
TGTGAAGAGA TCCTGGTGAA ACACATGGGC AGCCGCCTCT ATAGTGTCTC TTACCTGCTC 
AAGGACAAAG GGGAGTACAC ACTGGTGGTC AAGTGGGGTG ATGAGCATAT CCCAGGCAGC 
CCATACCGCA TTATGGTACC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527974.1
CDS	242..8185
Translation	MSSSHSRCGQSAAVASPGGSIDSRDAEMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVSKRIANLQTDLSDGLRLIALLEVLSQKKMHRKHNQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEDEEAKKQTPKQRLLGWIQNKLPQLPITNFSRDWQSGRALGALVDSCAPGLCPDWDSWDASKPVNNAREAMQQADDWLGIPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNMVKKRAEFTVETRSAGQGEVLVYVEDPAGHQEEAKVTANNDKNRTFSVWYVPEVTGTHKVTVLFAGQHIAKSPFEVYVDKSQGDASKVTAQGPGLEPSGNIANKTTYFEIFTAGAGMGEVEVVIQDPTGQKGTVEPQLEARGDSTYRCSYQPTMEGVHTVHVTFAGVPIPRSPYTVTVGQACNPAACRAIGRGLQPKGVRVKETADFKVYTKGAGSGELKVTVKGPKGEERVKQKDLGDGVYGFEYYPTIPGTYTVTITWGGQNIGRSPFEVKVGTECGNQKVRAWGPGLEGGIVGKSADFVVEAIGDDVGTLGFSVEGPSQAKIECDDKGDGSCDVRYWPQEAGEYAVHVLCNSEDIRLSPFMADIREAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAKHAGKAPLRVQVQDNEGCSVEATVKDNGNGTYSCSYVPRKPVKHTAMVSWGGVSIPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCTEAGQGDVSIGIKCAPGVVGPTEADIDFDIIRNDNDTFTVKYTPCGAGSYTIMVLFADQATPTSPIRVKVEPSHDASKVKAEGPGLNRTGVELGKPTHFTVNAKTAGKGKLDVQFSGLAKGDAVRDVDIIDHHDNTYTVKYIPVQQGPVGVNVTYGGDHIPKSPFSVGVSPSLDLSKIKVSGLGDKVDVGKDQEFTVKSKGAGGQGKVASKIVSPSGAAVPCKVEPGLGADNSVVRFVPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQGGNAGSPARFTIDTKGAGTGGLGLTVEGPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVAPCFDASKVKCSGPGLERATAGEVGQFQVDCSSAGSAELTIEICSEAGLPAEVYIQDHGDGTHTITYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTGGPHVKARVANPSGNLTDTYVQDCGDGTYKVEYTPYEEGVHSVDVTYDGSPVPSSPFQVPVTEGCDPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKMSCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDVVDNADGTQTVNYVPSREGSYSISVLYGEEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVPDVPGRYTILIKYGGDEIPFSPYRVRAVPTGDASKCTVTVSIGGHGLGAGIGPTIQIGEETVITVDTKAAGKGKVTCTVCTPDGSEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPTVQTPLRSQQLAPQYNYPQGSQQTWIPERPMVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPSITDNKDGTVTVRYSPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHITAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNDQHIPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANIGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGMHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEVGVPAEFGIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGIYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGSPAEFIVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKVATVPQHATSGPGPADVSKVVAKGLGLSKAYVGQKSNFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHMGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRIMVP

Target ORF information:

RefSeq Version	XM_006527911.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus filamin, alpha (Flna), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527911.4

ORF Insert Sequence:

ATGAGTAGCT CTCACTCCCG CTGTGGCCAG AGTGCGGCGG TTGCGTCTCC GGGAGGCAGT 
ATCGATTCAC GGGACGCGGA GATGCCGGCT ACCGAAAAAG ACCTAGCAGA AGATGCACCG 
TGGAAGAAAA TTCAGCAGAA CACATTCACC CGCTGGTGCA ATGAGCACCT TAAGTGCGTA 
AGCAAGCGCA TCGCCAATCT GCAGACGGAC CTGAGCGATG GGTTGCGGCT CATCGCGCTG 
CTCGAGGTAC TCAGCCAGAA GAAAATGCAC CGCAAGCACA ACCAAAGACC CACTTTCCGC 
CAGATGCAGC TCGAAAATGT GTCGGTGGCG CTTGAATTCC TGGACCGTGA GAGCATCAAG 
CTCGTGTCCA TAGACAGCAA GGCTATTGTG GATGGAAATC TGAAGCTGAT CTTAGGCCTC 
ATCTGGACCC TGATCCTGCA CTATTCCATC TCAATGCCCA TGTGGGATGA GGAAGAGGAT 
GAGGAGGCCA AGAAGCAAAC ACCCAAGCAG AGGCTTCTAG GCTGGATTCA GAACAAGCTA 
CCACAGCTTC CCATTACCAA CTTCAGTCGA GACTGGCAGA GTGGCCGGGC CCTGGGTGCT 
CTTGTTGATA GCTGTGCCCC AGGCCTATGT CCTGACTGGG ACTCCTGGGA TGCTAGTAAG 
CCTGTGAACA ATGCACGGGA AGCCATGCAG CAGGCTGATG ACTGGCTAGG CATTCCTCAG 
GTGATTACCC CAGAAGAAAT TGTGGACCCC AATGTAGATG AGCATTCTGT TATGACCTAC 
CTGTCTCAGT TTCCCAAGGC CAAGCTGAAG CCAGGGGCTC CTCTTCGGCC CAAACTGAAC 
CCGAAGAAAG CCCGAGCCTA TGGGCCAGGC ATCGAGCCTA CAGGCAATAT GGTGAAGAAG 
AGAGCAGAAT TCACTGTGGA GACCCGAAGT GCTGGACAGG GAGAAGTGCT TGTATATGTG 
GAGGACCCAG CTGGACACCA GGAAGAGGCA AAAGTGACTG CCAATAATGA CAAGAACCGT 
ACTTTCTCTG TCTGGTATGT CCCTGAAGTG ACAGGGACTC ATAAGGTGAC TGTGCTCTTT 
GCTGGCCAAC ATATTGCCAA GAGCCCCTTT GAGGTGTATG TGGACAAGTC ACAGGGTGAT 
GCCAGCAAAG TGACTGCCCA GGGCCCTGGT CTGGAGCCCA GTGGCAATAT TGCCAACAAG 
ACTACCTACT TTGAGATCTT CACTGCAGGA GCTGGCATGG GTGAGGTGGA AGTTGTCATC 
CAGGACCCTA CAGGACAGAA AGGCACAGTG GAACCTCAGC TGGAGGCCAG GGGTGACAGC 
ACCTATCGCT GTAGCTATCA GCCCACCATG GAGGGTGTCC ATACAGTACA TGTCACCTTC 
GCCGGTGTTC CCATCCCTCG TAGCCCCTAC ACTGTCACTG TTGGCCAAGC CTGTAACCCA 
GCTGCCTGCC GGGCTATTGG TAGAGGCCTT CAGCCCAAGG GTGTTCGAGT GAAGGAAACA 
GCCGACTTCA AGGTGTACAC AAAGGGCGCT GGCAGTGGGG AGCTAAAGGT CACTGTAAAG 
GGTCCCAAGG GTGAGGAGCG TGTAAAGCAG AAGGACTTAG GGGATGGTGT GTATGGCTTT 
GAATATTACC CTACAATCCC TGGCACATAC ACTGTCACCA TCACATGGGG TGGCCAGAAC 
ATTGGTCGAA GTCCGTTTGA GGTGAAGGTA GGCACTGAGT GTGGCAATCA GAAAGTTCGG 
GCATGGGGTC CTGGCCTGGA AGGAGGCATT GTTGGCAAGT CAGCAGACTT CGTAGTAGAG 
GCCATTGGTG ATGATGTGGG CACCTTGGGT TTCTCTGTGG AAGGTCCATC ACAGGCAAAG 
ATTGAATGTG ACGACAAGGG TGATGGCTCC TGTGATGTGC GCTATTGGCC CCAGGAGGCT 
GGCGAGTATG CTGTTCATGT GCTGTGTAAC AGTGAGGATA TCCGTCTCAG TCCTTTCATG 
GCTGACATCC GTGAGGCACC CCAGGATTTT CACCCAGACA GGGTGAAGGC ACGTGGGCCT 
GGATTGGAGA AGACTGGTGT GGCTGTCAAC AAGCCAGCAG AGTTCACAGT TGATGCCAAG 
CATGCTGGGA AGGCTCCTCT TCGAGTTCAA GTTCAGGACA ATGAGGGCTG CTCTGTGGAA 
GCGACAGTCA AGGACAATGG CAATGGTACT TACAGCTGTT CTTATGTGCC CAGAAAGCCA 
GTGAAGCACA CAGCCATGGT TTCTTGGGGA GGTGTCAGCA TCCCCAACAG TCCTTTCCGG 
GTGAATGTGG GAGCTGGCAG CCATCCAAAC AAAGTCAAGG TGTATGGTCC AGGAGTGGCC 
AAGACTGGGC TCAAGGCCCA TGAACCTACC TACTTTACTG TGGATTGTAC TGAAGCTGGC 
CAGGGAGATG TCAGCATTGG TATCAAGTGT GCCCCTGGAG TAGTGGGCCC CACTGAGGCT 
GATATTGACT TTGATATCAT CCGCAATGAC AATGACACCT TCACTGTAAA ATACACACCC 
TGTGGGGCTG GCAGCTATAC CATCATGGTC CTTTTTGCTG ACCAGGCCAC ACCCACCAGC 
CCCATCAGAG TCAAAGTGGA GCCTTCTCAT GATGCCAGCA AGGTGAAGGC TGAGGGTCCT 
GGCCTAAATC GCACTGGTGT TGAACTTGGC AAACCCACCC ATTTCACAGT CAATGCTAAA 
ACTGCTGGGA AAGGCAAGCT GGATGTCCAA TTCTCAGGAC TGGCTAAGGG AGATGCAGTA 
CGGGATGTGG ACATCATTGA CCACCATGAT AATACCTACA CAGTCAAGTA CATTCCTGTG 
CAGCAGGGCC CAGTAGGTGT CAATGTCACT TATGGAGGAG ATCACATCCC CAAGAGTCCA 
TTTTCAGTGG GAGTATCTCC AAGCCTGGAT CTCAGCAAAA TCAAGGTGTC TGGCCTTGGT 
GACAAAGTGG ACGTTGGCAA AGATCAAGAG TTCACAGTAA AGTCAAAGGG TGCAGGTGGT 
CAAGGCAAAG TAGCATCCAA GATTGTGAGT CCCTCAGGTG CAGCGGTACC CTGCAAGGTA 
GAGCCAGGCC TGGGAGCTGA CAACAGCGTG GTACGTTTTG TGCCCCGTGA AGAGGGGCCC 
TATGAGGTGG AAGTGACCTA TGATGGTGTG CCTGTACCTG GCAGTCCCTT TCCACTAGAA 
GCTGTGGCCC CCACCAAACC CAGCAAGGTG AAGGCGTTTG GACCAGGGCT ACAGGGGGGC 
AATGCAGGCT CCCCTGCCCG CTTCACCATT GATACAAAGG GTGCTGGCAC TGGTGGCCTG 
GGCCTGACAG TGGAAGGCCC CTGTGAAGCA CAGCTTGAGT GCCTAGACAA CGGGGATGGT 
ACATGCTCTG TGTCTTATGT ACCCACTGAG CCTGGGGACT ACAACATCAA CATCCTTTTT 
GCTGACACCC ACATTCCTGG ATCCCCATTC AAGGCCCATG TGGCTCCTTG TTTTGATGCA 
TCCAAGGTGA AGTGCTCAGG CCCTGGGCTG GAGCGGGCTA CTGCTGGTGA GGTAGGGCAG 
TTCCAAGTGG ACTGTTCAAG TGCTGGCAGT GCTGAGTTGA CGATTGAGAT CTGCTCTGAG 
GCAGGACTGC CAGCTGAAGT ATACATTCAA GACCATGGTG ATGGCACACA CACCATTACC 
TATATTCCTC TCTGTCCTGG GGCTTACACT GTTACCATCA AGTATGGCGG CCAGCCTGTG 
CCCAACTTCC CCAGCAAGCT ACAGGTGGAA CCTGCTGTAG ATACCTCAGG TGTACAGTGC 
TATGGGCCTG GGATTGAAGG TCAAGGTGTC TTCCGAGAGG CAACCACTGA GTTCAGTGTG 
GATGCCCGGG CTCTTACACA GACTGGAGGG CCACATGTCA AGGCTCGAGT GGCCAACCCC 
TCAGGCAATC TGACAGATAC CTATGTGCAA GACTGTGGGG ATGGCACATA CAAAGTGGAA 
TACACTCCAT ATGAGGAAGG AGTACACTCT GTGGATGTGA CTTATGATGG CAGCCCTGTG 
CCCAGCAGCC CCTTCCAGGT GCCTGTAACA GAGGGCTGTG ACCCCTCCCG GGTGCGTGTC 
CATGGACCAG GCATCCAAAG TGGTACCACC AACAAACCCA ACAAGTTCAC AGTAGAAACC 
AGGGGAGCTG GCACAGGTGG CCTGGGCTTG GCTGTTGAGG GTCCCTCAGA GGCCAAGATG 
TCTTGTATGG ATAATAAAGA TGGCAGCTGC TCAGTAGAAT ACATCCCCTA TGAAGCTGGA 
ACCTATAGCC TTAATGTCAC TTATGGTGGT CACCAAGTGC CAGGTAGTCC CTTCAAGGTC 
CCTGTACATG ATGTGACAGA TGCATCTAAA GTCAAGTGTT CTGGACCTGG CCTAAGCCCA 
GGCATGGTCC GTGCCAACCT CCCTCAGTCC TTTCAGGTGG ACACAAGCAA AGCTGGAGTT 
GCCCCACTGC AGGTCAAAGT GCAGGGGCCC AAAGGCCTGG TGGAGCCAGT GGATGTAGTG 
GACAATGCTG ATGGTACTCA GACTGTCAAC TATGTGCCCA GCCGAGAAGG GTCCTATAGC 
ATTTCTGTGC TGTATGGTGA AGAAGAAGTG CCACGGAGCC CCTTCAAGGT CAAGGTGCTG 
CCTACACATG ATGCCAGTAA GGTGAAGGCC AGTGGACCTG GACTCAACAC CACTGGTGTA 
CCTGCTAGCC TGCCTGTGGA GTTCACCATT GATGCCAAGG ATGCTGGGGA GGGTCTGTTG 
GCTGTCCAGA TTACGGATCC TGAAGGCAAG CCCAAGAAGA CACACATTCA AGATAATCAT 
GATGGCACAT ACACGGTGGC TTATGTGCCA GATGTGCCAG GCCGGTACAC AATCCTCATC 
AAGTATGGTG GTGATGAGAT TCCCTTTTCC CCGTACCGTG TCCGGGCTGT GCCCACTGGG 
GATGCCAGCA AGTGCACAGT CACAGTGTCA ATCGGAGGTC ACGGGCTAGG TGCTGGCATT 
GGCCCCACCA TCCAGATTGG GGAGGAGACG GTGATTACTG TGGACACAAA AGCAGCAGGC 
AAAGGCAAAG TGACTTGTAC TGTGTGCACA CCTGATGGCT CAGAGGTAGA CGTGGACGTG 
GTGGAGAATG AGGATGGCAC CTTTGACATC TTCTACACAG CTCCCCAACC GGGCAAATAT 
GTCATCTGTG TGCGCTTCGG TGGCGAGCAT GTGCCCAACA GCCCCTTCCA AGTTACAGCT 
TTGGCTGGGG ACCAACCAAC AGTGCAGACC CCATTAAGGT CTCAGCAGCT GGCTCCACAG 
TATAACTATC CTCAGGGTAG CCAGCAAACC TGGATTCCAG AGAGGCCCAT GGTGGGCGTT 
AATGGGCTGG ATGTGACCAG CCTGAGGCCC TTTGATCTTG TCATCCCCTT CACTATCAAG 
AAGGGGGAGA TCACTGGGGA AGTTCGAATG CCCTCAGGCA AGGTGGCCCA GCCTTCCATT 
ACTGATAACA AAGATGGCAC TGTTACTGTA CGTTACTCAC CCAGTGAAGC TGGCCTGCAT 
GAAATGGACA TTCGCTATGA CAATATGCAT ATCCCAGGAA GCCCTCTGCA GTTCTATGTT 
GATTATGTCA ACTGTGGCCA CATCACTGCT TATGGTCCTG GCCTTACCCA TGGAGTGGTC 
AACAAACCTG CCACCTTCAC TGTCAACACC AAGGATGCAG GAGAGGGGGG CTTGTCTCTG 
GCCATTGAGG GTCCATCTAA AGCAGAAATC AGTTGCACTG ACAACCAGGA TGGAACATGC 
AGTGTCTCTT ACCTGCCTGT ACTGCCTGGT GACTATAGCA TCCTAGTTAA GTACAATGAT 
CAACACATCC CAGGCAGTCC CTTTACTGCC AGAGTAACAG GTGACGATTC CATGCGTATG 
TCCCACCTAA AGGTGGGTTC TGCTGCTGAT ATCCCCATCA ATATCTCAGA AACAGACCTT 
AGCCTACTCA CAGCCACTGT GGTGCCACCT TCGGGTCGAG AGGAACCCTG TCTGCTGAAA 
CGTTTGCGAA ATGGCCACGT GGGGATTTCC TTCGTGCCCA AGGAGACAGG GGAGCACCTG 
GTACATGTGA AGAAGAATGG CCAGCATGTG GCAAGCAGTC CCATCCCAGT AGTGATCAGC 
CAGTCGGAGA TAGGTGATGC CAGCCGTGTG AGGGTCTCTG GTCAAGGTCT TCATGAAGGT 
CATACCTTTG AGCCTGCAGA GTTTATTATT GACACCAGAG ATGCAGGCTA CGGTGGGCTT 
AGTCTGTCCA TTGAGGGCCC TAGCAAAGTA GACATCAACA CAGAGGATCT GGAGGATGGC 
ACATGCAGGG TCACCTACTG TCCCACAGAG CCTGGAAACT ACATTATAAA CATCAAATTT 
GCTGACCAGC ATGTGCCTGG CAGTCCCTTT TCTGTGAAGG TGACAGGTGA GGGCCGGGTG 
AAAGAGAGTA TCACACGCAG GCGACGTGCC CCTTCTGTGG CCAATATTGG CAGTCATTGT 
GACCTCAGCC TGAAGATTCC TGAAATTAGC ATCCAAGATA TGACAGCCCA GGTGACCAGC 
CCATCAGGCA AGACCCATGA GGCAGAGATC GTAGAAGGAG AGAACCATAC TTACTGTATC 
CGATTTGTGC CTGCTGAGAT GGGAATGCAT ACAGTCAGTG TCAAGTACAA GGGCCAGCAT 
GTACCTGGGA GCCCCTTCCA GTTCACTGTG GGGCCTCTGG GGGAAGGGGG TGCTCACAAG 
GTCCGTGCTG GAGGCCCTGG CCTAGAGAGG GCTGAAGTTG GAGTGCCAGC GGAGTTCGGC 
ATTTGGACTA GGGAAGCTGG CGCTGGAGGC CTGGCCATTG CTGTTGAAGG CCCCAGCAAG 
GCTGAGATCT CTTTCGAGGA CCGAAAGGAT GGCTCCTGTG GTGTGGCCTA CGTAGTTCAG 
GAGCCAGGTG ACTATGAGGT CTCAGTCAAG TTCAACGAGG AGCACATACC TGATAGCCCC 
TTCGTGGTGC CTGTGGCTTC TCCGTCTGGT GACGCCCGCC GCCTTACTGT TTCTAGTCTT 
CAGGAGTCAG GGTTAAAGGT CAACCAGCCA GCATCTTTTG CAGTCAGTCT GAATGGAGCC 
AAGGGGGCAA TTGATGCCAA GGTGCACAGC CCCTCAGGAG CTCTGGAGGA GTGCTATGTC 
ACAGAGATTG ACCAAGATAA GTATGCTGTG CGTTTCATCC CACGAGAGAA TGGCATCTAC 
TTGATTGATG TCAAGTTCAA TGGTACTCAC ATTCCTGGAA GTCCCTTCAA GATCCGAGTT 
GGGGAGCCTG GGCATGGAGG GGACCCAGGC TTAGTGTCCG CCTATGGAGC AGGCCTGGAA 
GGTGGTGTCA CAGGGAGCCC AGCAGAGTTT ATTGTGAACA CAAGCAATGC AGGAGCTGGT 
GCCCTTTCGG TTACCATTGA TGGCCCCTCC AAGGTGAAGA TGGATTGCCA GGAGTGCCCC 
GAGGGCTATC GTGTCACCTA TACCCCCATG GCACCTGGCA GCTACCTCAT CTCCATCAAG 
TATGGTGGCC CCTATCACAT TGGTGGAAGT CCCTTTAAAG CCAAGGTCAC AGGTCCTCGT 
CTTGTAAGCA ACCACAGCCT CCATGAGACA TCATCTGTGT TTGTGGACTC TTTGACTAAA 
GTTGCCACGG TTCCCCAGCA TGCAACCTCA GGCCCAGGTC CTGCTGATGT CAGCAAGGTA 
GTAGCCAAAG GCCTGGGGCT AAGCAAGGCT TATGTAGGCC AGAAGAGCAA CTTCACAGTA 
GATTGCAGCA AAGCAGGTAA CAACATGCTG CTGGTGGGCG TGCATGGCCC AAGGACACCC 
TGTGAAGAGA TCCTGGTGAA ACACATGGGC AGCCGCCTCT ATAGTGTCTC TTACCTGCTC 
AAGGACAAAG GGGAGTACAC ACTGGTGGTC AAGTGGGGTG ATGAGCATAT CCCAGGCAGC 
CCATACCGCA TTATGGTACC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Flna ( XM_006527911.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527974.1 Mus musculus filamin, alpha (Flna), transcript variant X1, mRNA.

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CloneID	OMu56214
Clone ID Related Accession (Same CDS sequence)	XM_006527911.4 , XM_006527911.3
Accession Version	XM_006527911.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	filamin-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527911.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCT CTCACTCCCG CTGTGGCCAG AGTGCGGCGG TTGCGTCTCC GGGAGGCAGT 
ATCGATTCAC GGGACGCGGA GATGCCGGCT ACCGAAAAAG ACCTAGCAGA AGATGCACCG 
TGGAAGAAAA TTCAGCAGAA CACATTCACC CGCTGGTGCA ATGAGCACCT TAAGTGCGTA 
AGCAAGCGCA TCGCCAATCT GCAGACGGAC CTGAGCGATG GGTTGCGGCT CATCGCGCTG 
CTCGAGGTAC TCAGCCAGAA GAAAATGCAC CGCAAGCACA ACCAAAGACC CACTTTCCGC 
CAGATGCAGC TCGAAAATGT GTCGGTGGCG CTTGAATTCC TGGACCGTGA GAGCATCAAG 
CTCGTGTCCA TAGACAGCAA GGCTATTGTG GATGGAAATC TGAAGCTGAT CTTAGGCCTC 
ATCTGGACCC TGATCCTGCA CTATTCCATC TCAATGCCCA TGTGGGATGA GGAAGAGGAT 
GAGGAGGCCA AGAAGCAAAC ACCCAAGCAG AGGCTTCTAG GCTGGATTCA GAACAAGCTA 
CCACAGCTTC CCATTACCAA CTTCAGTCGA GACTGGCAGA GTGGCCGGGC CCTGGGTGCT 
CTTGTTGATA GCTGTGCCCC AGGCCTATGT CCTGACTGGG ACTCCTGGGA TGCTAGTAAG 
CCTGTGAACA ATGCACGGGA AGCCATGCAG CAGGCTGATG ACTGGCTAGG CATTCCTCAG 
GTGATTACCC CAGAAGAAAT TGTGGACCCC AATGTAGATG AGCATTCTGT TATGACCTAC 
CTGTCTCAGT TTCCCAAGGC CAAGCTGAAG CCAGGGGCTC CTCTTCGGCC CAAACTGAAC 
CCGAAGAAAG CCCGAGCCTA TGGGCCAGGC ATCGAGCCTA CAGGCAATAT GGTGAAGAAG 
AGAGCAGAAT TCACTGTGGA GACCCGAAGT GCTGGACAGG GAGAAGTGCT TGTATATGTG 
GAGGACCCAG CTGGACACCA GGAAGAGGCA AAAGTGACTG CCAATAATGA CAAGAACCGT 
ACTTTCTCTG TCTGGTATGT CCCTGAAGTG ACAGGGACTC ATAAGGTGAC TGTGCTCTTT 
GCTGGCCAAC ATATTGCCAA GAGCCCCTTT GAGGTGTATG TGGACAAGTC ACAGGGTGAT 
GCCAGCAAAG TGACTGCCCA GGGCCCTGGT CTGGAGCCCA GTGGCAATAT TGCCAACAAG 
ACTACCTACT TTGAGATCTT CACTGCAGGA GCTGGCATGG GTGAGGTGGA AGTTGTCATC 
CAGGACCCTA CAGGACAGAA AGGCACAGTG GAACCTCAGC TGGAGGCCAG GGGTGACAGC 
ACCTATCGCT GTAGCTATCA GCCCACCATG GAGGGTGTCC ATACAGTACA TGTCACCTTC 
GCCGGTGTTC CCATCCCTCG TAGCCCCTAC ACTGTCACTG TTGGCCAAGC CTGTAACCCA 
GCTGCCTGCC GGGCTATTGG TAGAGGCCTT CAGCCCAAGG GTGTTCGAGT GAAGGAAACA 
GCCGACTTCA AGGTGTACAC AAAGGGCGCT GGCAGTGGGG AGCTAAAGGT CACTGTAAAG 
GGTCCCAAGG GTGAGGAGCG TGTAAAGCAG AAGGACTTAG GGGATGGTGT GTATGGCTTT 
GAATATTACC CTACAATCCC TGGCACATAC ACTGTCACCA TCACATGGGG TGGCCAGAAC 
ATTGGTCGAA GTCCGTTTGA GGTGAAGGTA GGCACTGAGT GTGGCAATCA GAAAGTTCGG 
GCATGGGGTC CTGGCCTGGA AGGAGGCATT GTTGGCAAGT CAGCAGACTT CGTAGTAGAG 
GCCATTGGTG ATGATGTGGG CACCTTGGGT TTCTCTGTGG AAGGTCCATC ACAGGCAAAG 
ATTGAATGTG ACGACAAGGG TGATGGCTCC TGTGATGTGC GCTATTGGCC CCAGGAGGCT 
GGCGAGTATG CTGTTCATGT GCTGTGTAAC AGTGAGGATA TCCGTCTCAG TCCTTTCATG 
GCTGACATCC GTGAGGCACC CCAGGATTTT CACCCAGACA GGGTGAAGGC ACGTGGGCCT 
GGATTGGAGA AGACTGGTGT GGCTGTCAAC AAGCCAGCAG AGTTCACAGT TGATGCCAAG 
CATGCTGGGA AGGCTCCTCT TCGAGTTCAA GTTCAGGACA ATGAGGGCTG CTCTGTGGAA 
GCGACAGTCA AGGACAATGG CAATGGTACT TACAGCTGTT CTTATGTGCC CAGAAAGCCA 
GTGAAGCACA CAGCCATGGT TTCTTGGGGA GGTGTCAGCA TCCCCAACAG TCCTTTCCGG 
GTGAATGTGG GAGCTGGCAG CCATCCAAAC AAAGTCAAGG TGTATGGTCC AGGAGTGGCC 
AAGACTGGGC TCAAGGCCCA TGAACCTACC TACTTTACTG TGGATTGTAC TGAAGCTGGC 
CAGGGAGATG TCAGCATTGG TATCAAGTGT GCCCCTGGAG TAGTGGGCCC CACTGAGGCT 
GATATTGACT TTGATATCAT CCGCAATGAC AATGACACCT TCACTGTAAA ATACACACCC 
TGTGGGGCTG GCAGCTATAC CATCATGGTC CTTTTTGCTG ACCAGGCCAC ACCCACCAGC 
CCCATCAGAG TCAAAGTGGA GCCTTCTCAT GATGCCAGCA AGGTGAAGGC TGAGGGTCCT 
GGCCTAAATC GCACTGGTGT TGAACTTGGC AAACCCACCC ATTTCACAGT CAATGCTAAA 
ACTGCTGGGA AAGGCAAGCT GGATGTCCAA TTCTCAGGAC TGGCTAAGGG AGATGCAGTA 
CGGGATGTGG ACATCATTGA CCACCATGAT AATACCTACA CAGTCAAGTA CATTCCTGTG 
CAGCAGGGCC CAGTAGGTGT CAATGTCACT TATGGAGGAG ATCACATCCC CAAGAGTCCA 
TTTTCAGTGG GAGTATCTCC AAGCCTGGAT CTCAGCAAAA TCAAGGTGTC TGGCCTTGGT 
GACAAAGTGG ACGTTGGCAA AGATCAAGAG TTCACAGTAA AGTCAAAGGG TGCAGGTGGT 
CAAGGCAAAG TAGCATCCAA GATTGTGAGT CCCTCAGGTG CAGCGGTACC CTGCAAGGTA 
GAGCCAGGCC TGGGAGCTGA CAACAGCGTG GTACGTTTTG TGCCCCGTGA AGAGGGGCCC 
TATGAGGTGG AAGTGACCTA TGATGGTGTG CCTGTACCTG GCAGTCCCTT TCCACTAGAA 
GCTGTGGCCC CCACCAAACC CAGCAAGGTG AAGGCGTTTG GACCAGGGCT ACAGGGGGGC 
AATGCAGGCT CCCCTGCCCG CTTCACCATT GATACAAAGG GTGCTGGCAC TGGTGGCCTG 
GGCCTGACAG TGGAAGGCCC CTGTGAAGCA CAGCTTGAGT GCCTAGACAA CGGGGATGGT 
ACATGCTCTG TGTCTTATGT ACCCACTGAG CCTGGGGACT ACAACATCAA CATCCTTTTT 
GCTGACACCC ACATTCCTGG ATCCCCATTC AAGGCCCATG TGGCTCCTTG TTTTGATGCA 
TCCAAGGTGA AGTGCTCAGG CCCTGGGCTG GAGCGGGCTA CTGCTGGTGA GGTAGGGCAG 
TTCCAAGTGG ACTGTTCAAG TGCTGGCAGT GCTGAGTTGA CGATTGAGAT CTGCTCTGAG 
GCAGGACTGC CAGCTGAAGT ATACATTCAA GACCATGGTG ATGGCACACA CACCATTACC 
TATATTCCTC TCTGTCCTGG GGCTTACACT GTTACCATCA AGTATGGCGG CCAGCCTGTG 
CCCAACTTCC CCAGCAAGCT ACAGGTGGAA CCTGCTGTAG ATACCTCAGG TGTACAGTGC 
TATGGGCCTG GGATTGAAGG TCAAGGTGTC TTCCGAGAGG CAACCACTGA GTTCAGTGTG 
GATGCCCGGG CTCTTACACA GACTGGAGGG CCACATGTCA AGGCTCGAGT GGCCAACCCC 
TCAGGCAATC TGACAGATAC CTATGTGCAA GACTGTGGGG ATGGCACATA CAAAGTGGAA 
TACACTCCAT ATGAGGAAGG AGTACACTCT GTGGATGTGA CTTATGATGG CAGCCCTGTG 
CCCAGCAGCC CCTTCCAGGT GCCTGTAACA GAGGGCTGTG ACCCCTCCCG GGTGCGTGTC 
CATGGACCAG GCATCCAAAG TGGTACCACC AACAAACCCA ACAAGTTCAC AGTAGAAACC 
AGGGGAGCTG GCACAGGTGG CCTGGGCTTG GCTGTTGAGG GTCCCTCAGA GGCCAAGATG 
TCTTGTATGG ATAATAAAGA TGGCAGCTGC TCAGTAGAAT ACATCCCCTA TGAAGCTGGA 
ACCTATAGCC TTAATGTCAC TTATGGTGGT CACCAAGTGC CAGGTAGTCC CTTCAAGGTC 
CCTGTACATG ATGTGACAGA TGCATCTAAA GTCAAGTGTT CTGGACCTGG CCTAAGCCCA 
GGCATGGTCC GTGCCAACCT CCCTCAGTCC TTTCAGGTGG ACACAAGCAA AGCTGGAGTT 
GCCCCACTGC AGGTCAAAGT GCAGGGGCCC AAAGGCCTGG TGGAGCCAGT GGATGTAGTG 
GACAATGCTG ATGGTACTCA GACTGTCAAC TATGTGCCCA GCCGAGAAGG GTCCTATAGC 
ATTTCTGTGC TGTATGGTGA AGAAGAAGTG CCACGGAGCC CCTTCAAGGT CAAGGTGCTG 
CCTACACATG ATGCCAGTAA GGTGAAGGCC AGTGGACCTG GACTCAACAC CACTGGTGTA 
CCTGCTAGCC TGCCTGTGGA GTTCACCATT GATGCCAAGG ATGCTGGGGA GGGTCTGTTG 
GCTGTCCAGA TTACGGATCC TGAAGGCAAG CCCAAGAAGA CACACATTCA AGATAATCAT 
GATGGCACAT ACACGGTGGC TTATGTGCCA GATGTGCCAG GCCGGTACAC AATCCTCATC 
AAGTATGGTG GTGATGAGAT TCCCTTTTCC CCGTACCGTG TCCGGGCTGT GCCCACTGGG 
GATGCCAGCA AGTGCACAGT CACAGTGTCA ATCGGAGGTC ACGGGCTAGG TGCTGGCATT 
GGCCCCACCA TCCAGATTGG GGAGGAGACG GTGATTACTG TGGACACAAA AGCAGCAGGC 
AAAGGCAAAG TGACTTGTAC TGTGTGCACA CCTGATGGCT CAGAGGTAGA CGTGGACGTG 
GTGGAGAATG AGGATGGCAC CTTTGACATC TTCTACACAG CTCCCCAACC GGGCAAATAT 
GTCATCTGTG TGCGCTTCGG TGGCGAGCAT GTGCCCAACA GCCCCTTCCA AGTTACAGCT 
TTGGCTGGGG ACCAACCAAC AGTGCAGACC CCATTAAGGT CTCAGCAGCT GGCTCCACAG 
TATAACTATC CTCAGGGTAG CCAGCAAACC TGGATTCCAG AGAGGCCCAT GGTGGGCGTT 
AATGGGCTGG ATGTGACCAG CCTGAGGCCC TTTGATCTTG TCATCCCCTT CACTATCAAG 
AAGGGGGAGA TCACTGGGGA AGTTCGAATG CCCTCAGGCA AGGTGGCCCA GCCTTCCATT 
ACTGATAACA AAGATGGCAC TGTTACTGTA CGTTACTCAC CCAGTGAAGC TGGCCTGCAT 
GAAATGGACA TTCGCTATGA CAATATGCAT ATCCCAGGAA GCCCTCTGCA GTTCTATGTT 
GATTATGTCA ACTGTGGCCA CATCACTGCT TATGGTCCTG GCCTTACCCA TGGAGTGGTC 
AACAAACCTG CCACCTTCAC TGTCAACACC AAGGATGCAG GAGAGGGGGG CTTGTCTCTG 
GCCATTGAGG GTCCATCTAA AGCAGAAATC AGTTGCACTG ACAACCAGGA TGGAACATGC 
AGTGTCTCTT ACCTGCCTGT ACTGCCTGGT GACTATAGCA TCCTAGTTAA GTACAATGAT 
CAACACATCC CAGGCAGTCC CTTTACTGCC AGAGTAACAG GTGACGATTC CATGCGTATG 
TCCCACCTAA AGGTGGGTTC TGCTGCTGAT ATCCCCATCA ATATCTCAGA AACAGACCTT 
AGCCTACTCA CAGCCACTGT GGTGCCACCT TCGGGTCGAG AGGAACCCTG TCTGCTGAAA 
CGTTTGCGAA ATGGCCACGT GGGGATTTCC TTCGTGCCCA AGGAGACAGG GGAGCACCTG 
GTACATGTGA AGAAGAATGG CCAGCATGTG GCAAGCAGTC CCATCCCAGT AGTGATCAGC 
CAGTCGGAGA TAGGTGATGC CAGCCGTGTG AGGGTCTCTG GTCAAGGTCT TCATGAAGGT 
CATACCTTTG AGCCTGCAGA GTTTATTATT GACACCAGAG ATGCAGGCTA CGGTGGGCTT 
AGTCTGTCCA TTGAGGGCCC TAGCAAAGTA GACATCAACA CAGAGGATCT GGAGGATGGC 
ACATGCAGGG TCACCTACTG TCCCACAGAG CCTGGAAACT ACATTATAAA CATCAAATTT 
GCTGACCAGC ATGTGCCTGG CAGTCCCTTT TCTGTGAAGG TGACAGGTGA GGGCCGGGTG 
AAAGAGAGTA TCACACGCAG GCGACGTGCC CCTTCTGTGG CCAATATTGG CAGTCATTGT 
GACCTCAGCC TGAAGATTCC TGAAATTAGC ATCCAAGATA TGACAGCCCA GGTGACCAGC 
CCATCAGGCA AGACCCATGA GGCAGAGATC GTAGAAGGAG AGAACCATAC TTACTGTATC 
CGATTTGTGC CTGCTGAGAT GGGAATGCAT ACAGTCAGTG TCAAGTACAA GGGCCAGCAT 
GTACCTGGGA GCCCCTTCCA GTTCACTGTG GGGCCTCTGG GGGAAGGGGG TGCTCACAAG 
GTCCGTGCTG GAGGCCCTGG CCTAGAGAGG GCTGAAGTTG GAGTGCCAGC GGAGTTCGGC 
ATTTGGACTA GGGAAGCTGG CGCTGGAGGC CTGGCCATTG CTGTTGAAGG CCCCAGCAAG 
GCTGAGATCT CTTTCGAGGA CCGAAAGGAT GGCTCCTGTG GTGTGGCCTA CGTAGTTCAG 
GAGCCAGGTG ACTATGAGGT CTCAGTCAAG TTCAACGAGG AGCACATACC TGATAGCCCC 
TTCGTGGTGC CTGTGGCTTC TCCGTCTGGT GACGCCCGCC GCCTTACTGT TTCTAGTCTT 
CAGGAGTCAG GGTTAAAGGT CAACCAGCCA GCATCTTTTG CAGTCAGTCT GAATGGAGCC 
AAGGGGGCAA TTGATGCCAA GGTGCACAGC CCCTCAGGAG CTCTGGAGGA GTGCTATGTC 
ACAGAGATTG ACCAAGATAA GTATGCTGTG CGTTTCATCC CACGAGAGAA TGGCATCTAC 
TTGATTGATG TCAAGTTCAA TGGTACTCAC ATTCCTGGAA GTCCCTTCAA GATCCGAGTT 
GGGGAGCCTG GGCATGGAGG GGACCCAGGC TTAGTGTCCG CCTATGGAGC AGGCCTGGAA 
GGTGGTGTCA CAGGGAGCCC AGCAGAGTTT ATTGTGAACA CAAGCAATGC AGGAGCTGGT 
GCCCTTTCGG TTACCATTGA TGGCCCCTCC AAGGTGAAGA TGGATTGCCA GGAGTGCCCC 
GAGGGCTATC GTGTCACCTA TACCCCCATG GCACCTGGCA GCTACCTCAT CTCCATCAAG 
TATGGTGGCC CCTATCACAT TGGTGGAAGT CCCTTTAAAG CCAAGGTCAC AGGTCCTCGT 
CTTGTAAGCA ACCACAGCCT CCATGAGACA TCATCTGTGT TTGTGGACTC TTTGACTAAA 
GTTGCCACGG TTCCCCAGCA TGCAACCTCA GGCCCAGGTC CTGCTGATGT CAGCAAGGTA 
GTAGCCAAAG GCCTGGGGCT AAGCAAGGCT TATGTAGGCC AGAAGAGCAA CTTCACAGTA 
GATTGCAGCA AAGCAGGTAA CAACATGCTG CTGGTGGGCG TGCATGGCCC AAGGACACCC 
TGTGAAGAGA TCCTGGTGAA ACACATGGGC AGCCGCCTCT ATAGTGTCTC TTACCTGCTC 
AAGGACAAAG GGGAGTACAC ACTGGTGGTC AAGTGGGGTG ATGAGCATAT CCCAGGCAGC 
CCATACCGCA TTATGGTACC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527974.1
CDS	240..8183
Translation	MSSSHSRCGQSAAVASPGGSIDSRDAEMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVSKRIANLQTDLSDGLRLIALLEVLSQKKMHRKHNQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEDEEAKKQTPKQRLLGWIQNKLPQLPITNFSRDWQSGRALGALVDSCAPGLCPDWDSWDASKPVNNAREAMQQADDWLGIPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNMVKKRAEFTVETRSAGQGEVLVYVEDPAGHQEEAKVTANNDKNRTFSVWYVPEVTGTHKVTVLFAGQHIAKSPFEVYVDKSQGDASKVTAQGPGLEPSGNIANKTTYFEIFTAGAGMGEVEVVIQDPTGQKGTVEPQLEARGDSTYRCSYQPTMEGVHTVHVTFAGVPIPRSPYTVTVGQACNPAACRAIGRGLQPKGVRVKETADFKVYTKGAGSGELKVTVKGPKGEERVKQKDLGDGVYGFEYYPTIPGTYTVTITWGGQNIGRSPFEVKVGTECGNQKVRAWGPGLEGGIVGKSADFVVEAIGDDVGTLGFSVEGPSQAKIECDDKGDGSCDVRYWPQEAGEYAVHVLCNSEDIRLSPFMADIREAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAKHAGKAPLRVQVQDNEGCSVEATVKDNGNGTYSCSYVPRKPVKHTAMVSWGGVSIPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCTEAGQGDVSIGIKCAPGVVGPTEADIDFDIIRNDNDTFTVKYTPCGAGSYTIMVLFADQATPTSPIRVKVEPSHDASKVKAEGPGLNRTGVELGKPTHFTVNAKTAGKGKLDVQFSGLAKGDAVRDVDIIDHHDNTYTVKYIPVQQGPVGVNVTYGGDHIPKSPFSVGVSPSLDLSKIKVSGLGDKVDVGKDQEFTVKSKGAGGQGKVASKIVSPSGAAVPCKVEPGLGADNSVVRFVPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQGGNAGSPARFTIDTKGAGTGGLGLTVEGPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVAPCFDASKVKCSGPGLERATAGEVGQFQVDCSSAGSAELTIEICSEAGLPAEVYIQDHGDGTHTITYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTGGPHVKARVANPSGNLTDTYVQDCGDGTYKVEYTPYEEGVHSVDVTYDGSPVPSSPFQVPVTEGCDPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKMSCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDVVDNADGTQTVNYVPSREGSYSISVLYGEEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVPDVPGRYTILIKYGGDEIPFSPYRVRAVPTGDASKCTVTVSIGGHGLGAGIGPTIQIGEETVITVDTKAAGKGKVTCTVCTPDGSEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPTVQTPLRSQQLAPQYNYPQGSQQTWIPERPMVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPSITDNKDGTVTVRYSPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHITAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNDQHIPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANIGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGMHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEVGVPAEFGIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGIYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGSPAEFIVNTSNAGAGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKVATVPQHATSGPGPADVSKVVAKGLGLSKAYVGQKSNFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHMGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRIMVP

Target ORF information:

RefSeq Version	XM_006527911.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus filamin, alpha (Flna), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527911.3

ORF Insert Sequence:

ATGAGTAGCT CTCACTCCCG CTGTGGCCAG AGTGCGGCGG TTGCGTCTCC GGGAGGCAGT 
ATCGATTCAC GGGACGCGGA GATGCCGGCT ACCGAAAAAG ACCTAGCAGA AGATGCACCG 
TGGAAGAAAA TTCAGCAGAA CACATTCACC CGCTGGTGCA ATGAGCACCT TAAGTGCGTA 
AGCAAGCGCA TCGCCAATCT GCAGACGGAC CTGAGCGATG GGTTGCGGCT CATCGCGCTG 
CTCGAGGTAC TCAGCCAGAA GAAAATGCAC CGCAAGCACA ACCAAAGACC CACTTTCCGC 
CAGATGCAGC TCGAAAATGT GTCGGTGGCG CTTGAATTCC TGGACCGTGA GAGCATCAAG 
CTCGTGTCCA TAGACAGCAA GGCTATTGTG GATGGAAATC TGAAGCTGAT CTTAGGCCTC 
ATCTGGACCC TGATCCTGCA CTATTCCATC TCAATGCCCA TGTGGGATGA GGAAGAGGAT 
GAGGAGGCCA AGAAGCAAAC ACCCAAGCAG AGGCTTCTAG GCTGGATTCA GAACAAGCTA 
CCACAGCTTC CCATTACCAA CTTCAGTCGA GACTGGCAGA GTGGCCGGGC CCTGGGTGCT 
CTTGTTGATA GCTGTGCCCC AGGCCTATGT CCTGACTGGG ACTCCTGGGA TGCTAGTAAG 
CCTGTGAACA ATGCACGGGA AGCCATGCAG CAGGCTGATG ACTGGCTAGG CATTCCTCAG 
GTGATTACCC CAGAAGAAAT TGTGGACCCC AATGTAGATG AGCATTCTGT TATGACCTAC 
CTGTCTCAGT TTCCCAAGGC CAAGCTGAAG CCAGGGGCTC CTCTTCGGCC CAAACTGAAC 
CCGAAGAAAG CCCGAGCCTA TGGGCCAGGC ATCGAGCCTA CAGGCAATAT GGTGAAGAAG 
AGAGCAGAAT TCACTGTGGA GACCCGAAGT GCTGGACAGG GAGAAGTGCT TGTATATGTG 
GAGGACCCAG CTGGACACCA GGAAGAGGCA AAAGTGACTG CCAATAATGA CAAGAACCGT 
ACTTTCTCTG TCTGGTATGT CCCTGAAGTG ACAGGGACTC ATAAGGTGAC TGTGCTCTTT 
GCTGGCCAAC ATATTGCCAA GAGCCCCTTT GAGGTGTATG TGGACAAGTC ACAGGGTGAT 
GCCAGCAAAG TGACTGCCCA GGGCCCTGGT CTGGAGCCCA GTGGCAATAT TGCCAACAAG 
ACTACCTACT TTGAGATCTT CACTGCAGGA GCTGGCATGG GTGAGGTGGA AGTTGTCATC 
CAGGACCCTA CAGGACAGAA AGGCACAGTG GAACCTCAGC TGGAGGCCAG GGGTGACAGC 
ACCTATCGCT GTAGCTATCA GCCCACCATG GAGGGTGTCC ATACAGTACA TGTCACCTTC 
GCCGGTGTTC CCATCCCTCG TAGCCCCTAC ACTGTCACTG TTGGCCAAGC CTGTAACCCA 
GCTGCCTGCC GGGCTATTGG TAGAGGCCTT CAGCCCAAGG GTGTTCGAGT GAAGGAAACA 
GCCGACTTCA AGGTGTACAC AAAGGGCGCT GGCAGTGGGG AGCTAAAGGT CACTGTAAAG 
GGTCCCAAGG GTGAGGAGCG TGTAAAGCAG AAGGACTTAG GGGATGGTGT GTATGGCTTT 
GAATATTACC CTACAATCCC TGGCACATAC ACTGTCACCA TCACATGGGG TGGCCAGAAC 
ATTGGTCGAA GTCCGTTTGA GGTGAAGGTA GGCACTGAGT GTGGCAATCA GAAAGTTCGG 
GCATGGGGTC CTGGCCTGGA AGGAGGCATT GTTGGCAAGT CAGCAGACTT CGTAGTAGAG 
GCCATTGGTG ATGATGTGGG CACCTTGGGT TTCTCTGTGG AAGGTCCATC ACAGGCAAAG 
ATTGAATGTG ACGACAAGGG TGATGGCTCC TGTGATGTGC GCTATTGGCC CCAGGAGGCT 
GGCGAGTATG CTGTTCATGT GCTGTGTAAC AGTGAGGATA TCCGTCTCAG TCCTTTCATG 
GCTGACATCC GTGAGGCACC CCAGGATTTT CACCCAGACA GGGTGAAGGC ACGTGGGCCT 
GGATTGGAGA AGACTGGTGT GGCTGTCAAC AAGCCAGCAG AGTTCACAGT TGATGCCAAG 
CATGCTGGGA AGGCTCCTCT TCGAGTTCAA GTTCAGGACA ATGAGGGCTG CTCTGTGGAA 
GCGACAGTCA AGGACAATGG CAATGGTACT TACAGCTGTT CTTATGTGCC CAGAAAGCCA 
GTGAAGCACA CAGCCATGGT TTCTTGGGGA GGTGTCAGCA TCCCCAACAG TCCTTTCCGG 
GTGAATGTGG GAGCTGGCAG CCATCCAAAC AAAGTCAAGG TGTATGGTCC AGGAGTGGCC 
AAGACTGGGC TCAAGGCCCA TGAACCTACC TACTTTACTG TGGATTGTAC TGAAGCTGGC 
CAGGGAGATG TCAGCATTGG TATCAAGTGT GCCCCTGGAG TAGTGGGCCC CACTGAGGCT 
GATATTGACT TTGATATCAT CCGCAATGAC AATGACACCT TCACTGTAAA ATACACACCC 
TGTGGGGCTG GCAGCTATAC CATCATGGTC CTTTTTGCTG ACCAGGCCAC ACCCACCAGC 
CCCATCAGAG TCAAAGTGGA GCCTTCTCAT GATGCCAGCA AGGTGAAGGC TGAGGGTCCT 
GGCCTAAATC GCACTGGTGT TGAACTTGGC AAACCCACCC ATTTCACAGT CAATGCTAAA 
ACTGCTGGGA AAGGCAAGCT GGATGTCCAA TTCTCAGGAC TGGCTAAGGG AGATGCAGTA 
CGGGATGTGG ACATCATTGA CCACCATGAT AATACCTACA CAGTCAAGTA CATTCCTGTG 
CAGCAGGGCC CAGTAGGTGT CAATGTCACT TATGGAGGAG ATCACATCCC CAAGAGTCCA 
TTTTCAGTGG GAGTATCTCC AAGCCTGGAT CTCAGCAAAA TCAAGGTGTC TGGCCTTGGT 
GACAAAGTGG ACGTTGGCAA AGATCAAGAG TTCACAGTAA AGTCAAAGGG TGCAGGTGGT 
CAAGGCAAAG TAGCATCCAA GATTGTGAGT CCCTCAGGTG CAGCGGTACC CTGCAAGGTA 
GAGCCAGGCC TGGGAGCTGA CAACAGCGTG GTACGTTTTG TGCCCCGTGA AGAGGGGCCC 
TATGAGGTGG AAGTGACCTA TGATGGTGTG CCTGTACCTG GCAGTCCCTT TCCACTAGAA 
GCTGTGGCCC CCACCAAACC CAGCAAGGTG AAGGCGTTTG GACCAGGGCT ACAGGGGGGC 
AATGCAGGCT CCCCTGCCCG CTTCACCATT GATACAAAGG GTGCTGGCAC TGGTGGCCTG 
GGCCTGACAG TGGAAGGCCC CTGTGAAGCA CAGCTTGAGT GCCTAGACAA CGGGGATGGT 
ACATGCTCTG TGTCTTATGT ACCCACTGAG CCTGGGGACT ACAACATCAA CATCCTTTTT 
GCTGACACCC ACATTCCTGG ATCCCCATTC AAGGCCCATG TGGCTCCTTG TTTTGATGCA 
TCCAAGGTGA AGTGCTCAGG CCCTGGGCTG GAGCGGGCTA CTGCTGGTGA GGTAGGGCAG 
TTCCAAGTGG ACTGTTCAAG TGCTGGCAGT GCTGAGTTGA CGATTGAGAT CTGCTCTGAG 
GCAGGACTGC CAGCTGAAGT ATACATTCAA GACCATGGTG ATGGCACACA CACCATTACC 
TATATTCCTC TCTGTCCTGG GGCTTACACT GTTACCATCA AGTATGGCGG CCAGCCTGTG 
CCCAACTTCC CCAGCAAGCT ACAGGTGGAA CCTGCTGTAG ATACCTCAGG TGTACAGTGC 
TATGGGCCTG GGATTGAAGG TCAAGGTGTC TTCCGAGAGG CAACCACTGA GTTCAGTGTG 
GATGCCCGGG CTCTTACACA GACTGGAGGG CCACATGTCA AGGCTCGAGT GGCCAACCCC 
TCAGGCAATC TGACAGATAC CTATGTGCAA GACTGTGGGG ATGGCACATA CAAAGTGGAA 
TACACTCCAT ATGAGGAAGG AGTACACTCT GTGGATGTGA CTTATGATGG CAGCCCTGTG 
CCCAGCAGCC CCTTCCAGGT GCCTGTAACA GAGGGCTGTG ACCCCTCCCG GGTGCGTGTC 
CATGGACCAG GCATCCAAAG TGGTACCACC AACAAACCCA ACAAGTTCAC AGTAGAAACC 
AGGGGAGCTG GCACAGGTGG CCTGGGCTTG GCTGTTGAGG GTCCCTCAGA GGCCAAGATG 
TCTTGTATGG ATAATAAAGA TGGCAGCTGC TCAGTAGAAT ACATCCCCTA TGAAGCTGGA 
ACCTATAGCC TTAATGTCAC TTATGGTGGT CACCAAGTGC CAGGTAGTCC CTTCAAGGTC 
CCTGTACATG ATGTGACAGA TGCATCTAAA GTCAAGTGTT CTGGACCTGG CCTAAGCCCA 
GGCATGGTCC GTGCCAACCT CCCTCAGTCC TTTCAGGTGG ACACAAGCAA AGCTGGAGTT 
GCCCCACTGC AGGTCAAAGT GCAGGGGCCC AAAGGCCTGG TGGAGCCAGT GGATGTAGTG 
GACAATGCTG ATGGTACTCA GACTGTCAAC TATGTGCCCA GCCGAGAAGG GTCCTATAGC 
ATTTCTGTGC TGTATGGTGA AGAAGAAGTG CCACGGAGCC CCTTCAAGGT CAAGGTGCTG 
CCTACACATG ATGCCAGTAA GGTGAAGGCC AGTGGACCTG GACTCAACAC CACTGGTGTA 
CCTGCTAGCC TGCCTGTGGA GTTCACCATT GATGCCAAGG ATGCTGGGGA GGGTCTGTTG 
GCTGTCCAGA TTACGGATCC TGAAGGCAAG CCCAAGAAGA CACACATTCA AGATAATCAT 
GATGGCACAT ACACGGTGGC TTATGTGCCA GATGTGCCAG GCCGGTACAC AATCCTCATC 
AAGTATGGTG GTGATGAGAT TCCCTTTTCC CCGTACCGTG TCCGGGCTGT GCCCACTGGG 
GATGCCAGCA AGTGCACAGT CACAGTGTCA ATCGGAGGTC ACGGGCTAGG TGCTGGCATT 
GGCCCCACCA TCCAGATTGG GGAGGAGACG GTGATTACTG TGGACACAAA AGCAGCAGGC 
AAAGGCAAAG TGACTTGTAC TGTGTGCACA CCTGATGGCT CAGAGGTAGA CGTGGACGTG 
GTGGAGAATG AGGATGGCAC CTTTGACATC TTCTACACAG CTCCCCAACC GGGCAAATAT 
GTCATCTGTG TGCGCTTCGG TGGCGAGCAT GTGCCCAACA GCCCCTTCCA AGTTACAGCT 
TTGGCTGGGG ACCAACCAAC AGTGCAGACC CCATTAAGGT CTCAGCAGCT GGCTCCACAG 
TATAACTATC CTCAGGGTAG CCAGCAAACC TGGATTCCAG AGAGGCCCAT GGTGGGCGTT 
AATGGGCTGG ATGTGACCAG CCTGAGGCCC TTTGATCTTG TCATCCCCTT CACTATCAAG 
AAGGGGGAGA TCACTGGGGA AGTTCGAATG CCCTCAGGCA AGGTGGCCCA GCCTTCCATT 
ACTGATAACA AAGATGGCAC TGTTACTGTA CGTTACTCAC CCAGTGAAGC TGGCCTGCAT 
GAAATGGACA TTCGCTATGA CAATATGCAT ATCCCAGGAA GCCCTCTGCA GTTCTATGTT 
GATTATGTCA ACTGTGGCCA CATCACTGCT TATGGTCCTG GCCTTACCCA TGGAGTGGTC 
AACAAACCTG CCACCTTCAC TGTCAACACC AAGGATGCAG GAGAGGGGGG CTTGTCTCTG 
GCCATTGAGG GTCCATCTAA AGCAGAAATC AGTTGCACTG ACAACCAGGA TGGAACATGC 
AGTGTCTCTT ACCTGCCTGT ACTGCCTGGT GACTATAGCA TCCTAGTTAA GTACAATGAT 
CAACACATCC CAGGCAGTCC CTTTACTGCC AGAGTAACAG GTGACGATTC CATGCGTATG 
TCCCACCTAA AGGTGGGTTC TGCTGCTGAT ATCCCCATCA ATATCTCAGA AACAGACCTT 
AGCCTACTCA CAGCCACTGT GGTGCCACCT TCGGGTCGAG AGGAACCCTG TCTGCTGAAA 
CGTTTGCGAA ATGGCCACGT GGGGATTTCC TTCGTGCCCA AGGAGACAGG GGAGCACCTG 
GTACATGTGA AGAAGAATGG CCAGCATGTG GCAAGCAGTC CCATCCCAGT AGTGATCAGC 
CAGTCGGAGA TAGGTGATGC CAGCCGTGTG AGGGTCTCTG GTCAAGGTCT TCATGAAGGT 
CATACCTTTG AGCCTGCAGA GTTTATTATT GACACCAGAG ATGCAGGCTA CGGTGGGCTT 
AGTCTGTCCA TTGAGGGCCC TAGCAAAGTA GACATCAACA CAGAGGATCT GGAGGATGGC 
ACATGCAGGG TCACCTACTG TCCCACAGAG CCTGGAAACT ACATTATAAA CATCAAATTT 
GCTGACCAGC ATGTGCCTGG CAGTCCCTTT TCTGTGAAGG TGACAGGTGA GGGCCGGGTG 
AAAGAGAGTA TCACACGCAG GCGACGTGCC CCTTCTGTGG CCAATATTGG CAGTCATTGT 
GACCTCAGCC TGAAGATTCC TGAAATTAGC ATCCAAGATA TGACAGCCCA GGTGACCAGC 
CCATCAGGCA AGACCCATGA GGCAGAGATC GTAGAAGGAG AGAACCATAC TTACTGTATC 
CGATTTGTGC CTGCTGAGAT GGGAATGCAT ACAGTCAGTG TCAAGTACAA GGGCCAGCAT 
GTACCTGGGA GCCCCTTCCA GTTCACTGTG GGGCCTCTGG GGGAAGGGGG TGCTCACAAG 
GTCCGTGCTG GAGGCCCTGG CCTAGAGAGG GCTGAAGTTG GAGTGCCAGC GGAGTTCGGC 
ATTTGGACTA GGGAAGCTGG CGCTGGAGGC CTGGCCATTG CTGTTGAAGG CCCCAGCAAG 
GCTGAGATCT CTTTCGAGGA CCGAAAGGAT GGCTCCTGTG GTGTGGCCTA CGTAGTTCAG 
GAGCCAGGTG ACTATGAGGT CTCAGTCAAG TTCAACGAGG AGCACATACC TGATAGCCCC 
TTCGTGGTGC CTGTGGCTTC TCCGTCTGGT GACGCCCGCC GCCTTACTGT TTCTAGTCTT 
CAGGAGTCAG GGTTAAAGGT CAACCAGCCA GCATCTTTTG CAGTCAGTCT GAATGGAGCC 
AAGGGGGCAA TTGATGCCAA GGTGCACAGC CCCTCAGGAG CTCTGGAGGA GTGCTATGTC 
ACAGAGATTG ACCAAGATAA GTATGCTGTG CGTTTCATCC CACGAGAGAA TGGCATCTAC 
TTGATTGATG TCAAGTTCAA TGGTACTCAC ATTCCTGGAA GTCCCTTCAA GATCCGAGTT 
GGGGAGCCTG GGCATGGAGG GGACCCAGGC TTAGTGTCCG CCTATGGAGC AGGCCTGGAA 
GGTGGTGTCA CAGGGAGCCC AGCAGAGTTT ATTGTGAACA CAAGCAATGC AGGAGCTGGT 
GCCCTTTCGG TTACCATTGA TGGCCCCTCC AAGGTGAAGA TGGATTGCCA GGAGTGCCCC 
GAGGGCTATC GTGTCACCTA TACCCCCATG GCACCTGGCA GCTACCTCAT CTCCATCAAG 
TATGGTGGCC CCTATCACAT TGGTGGAAGT CCCTTTAAAG CCAAGGTCAC AGGTCCTCGT 
CTTGTAAGCA ACCACAGCCT CCATGAGACA TCATCTGTGT TTGTGGACTC TTTGACTAAA 
GTTGCCACGG TTCCCCAGCA TGCAACCTCA GGCCCAGGTC CTGCTGATGT CAGCAAGGTA 
GTAGCCAAAG GCCTGGGGCT AAGCAAGGCT TATGTAGGCC AGAAGAGCAA CTTCACAGTA 
GATTGCAGCA AAGCAGGTAA CAACATGCTG CTGGTGGGCG TGCATGGCCC AAGGACACCC 
TGTGAAGAGA TCCTGGTGAA ACACATGGGC AGCCGCCTCT ATAGTGTCTC TTACCTGCTC 
AAGGACAAAG GGGAGTACAC ACTGGTGGTC AAGTGGGGTG ATGAGCATAT CCCAGGCAGC 
CCATACCGCA TTATGGTACC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hypoxia-induced interaction of filamin with Drp1 causes mitochondrial hyperfission-associated myocardial senescence.
Science signaling11(556)(2018 11)
Nishimura A,Shimauchi T,Tanaka T,Shimoda K,Toyama T,Kitajima N,Ishikawa T,Shindo N,Numaga-Tomita T,Yasuda S,Sato Y,Kuwahara K,Kumagai Y,Akaike T,Ide T,Ojida A,Mori Y,Nishida M

RNA editing of Filamin A pre-mRNA regulates vascular contraction and diastolic blood pressure.
The EMBO journal37(19)(2018 10)
Jain M,Mann TD,Stulić M,Rao SP,Kirsch A,Pullirsch D,Strobl X,Rath C,Reissig L,Moreth K,Klein-Rodewald T,Bekeredjian R,Gailus-Durner V,Fuchs H,Hrabě de Angelis M,Pablik E,Cimatti L,Martin D,Zinnanti J,Graier WF,Sibilia M,Frank S,Levanon EY,Jantsch MF

IRE1α governs cytoskeleton remodelling and cell migration through a direct interaction with filamin A.
Nature cell biology20(8)942-953(2018 08)
Urra H,Henriquez DR,Cánovas J,Villarroel-Campos D,Carreras-Sureda A,Pulgar E,Molina E,Hazari YM,Limia CM,Alvarez-Rojas S,Figueroa R,Vidal RL,Rodriguez DA,Rivera CA,Court FA,Couve A,Qi L,Chevet E,Akai R,Iwawaki T,Concha ML,Glavic Á,Gonzalez-Billault C,Hetz C

Filamin A Is Required for Optimal T Cell Integrin-Mediated Force Transmission, Flow Adhesion, and T Cell Trafficking.
Journal of immunology (Baltimore, Md. : 1950)200(9)3109-3116(2018 05)
Savinko T,Guenther C,Uotila LM,Llort Asens M,Yao S,Tojkander S,Fagerholm SC

Filamin A Phosphorylation at Serine 2152 by the Serine/Threonine Kinase Ndr2 Controls TCR-Induced LFA-1 Activation in T Cells.
Frontiers in immunology92852(2018)
Waldt N,Seifert A,Demiray YE,Devroe E,Turk BE,Reichardt P,Mix C,Reinhold A,Freund C,Müller AJ,Schraven B,Stork O,Kliche S

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Flna cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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