Eif4a3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif4a3
Entrez Gene ID	192170
Full Name	eukaryotic translation initiation factor 4A3
Synonyms	2400003O03Rik,Ddx48,eIF4A-III,mKIAA0111
General protein information	Preferred Names eukaryotic translation initiation factor 4A3 Names eukaryotic initiation factor 4A-III ATP-dependent RNA helicase DDX48 ATP-dependent RNA helicase eIF4A-3 DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 DEAD box protein 48 eIF-4A-III eukaryotic translation initiation factor 4A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_138669.1	NP_619610.1	eukaryotic initiation factor 4A-III

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EIF4A3	XP_533130.2
Bos taurus (cattle)	EIF4A3	NP_001039653.1
Caenorhabditis elegans (roundworm)	F33D11.10	NP_491703.1
Saccharomyces cerevisiae (baker's yeast)	FAL1	NP_010304.3
Arabidopsis thaliana (thale cress)	EIF4A-III	NP_188610.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003089	XP_312776.3
Neurospora crassa	NCU01234	XP_961600.1
Danio rerio (zebrafish)	eif4a3	NP_957372.1
Drosophila melanogaster (fruit fly)	eIF4AIII	NP_649788.2
Caenorhabditis elegans (roundworm)	Y65B4A.6	NP_490761.2
Gallus gallus (chicken)	EIF4A3	NP_001025820.1
Oryza sativa (rice)	Os01g0639100	NP_001043673.1
Homo sapiens (human)	EIF4A3	NP_055555.1
Macaca mulatta (Rhesus monkey)	EIF4A3	XP_001110130.1
Mus musculus (house mouse)	Eif4a3	NP_619610.1
Schizosaccharomyces pombe (fission yeast)	SPAC1F5.10	NP_592863.1
Oryza sativa (rice)	Os03g0566800	NP_001050506.1
Rattus norvegicus (Norway rat)	Eif4a3	NP_001093628.1
Xenopus tropicalis (tropical clawed frog)	eif4a3	NP_001107349.1
Kluyveromyces lactis	KLLA0A10659g	XP_451466.1
Pan troglodytes (chimpanzee)	EIF4A3	XP_511724.3
Magnaporthe oryzae (rice blast fungus)	MGG_04885	XP_003712368.1
Eremothecium gossypii	AGOS_AER408W	NP_985263.2

Gene Ontology
Bibliography

Related articles in PubMed

EIF4A3 deficient human iPSCs and mouse models demonstrate neural crest defects that underlie Richieri-Costa-Pereira syndrome.
Miller EE, Kobayashi GS, Musso CM, Allen M, Ishiy FAA, de Caires LC Jr, Goulart E, Griesi-Oliveira K, Zechi-Ceide RM, Richieri-Costa A, Bertola DR, Passos-Bueno MR, Silver DL
Human molecular genetics26(12)2177-2191(2017 06)

Haploinsufficiency for Core Exon Junction Complex Components Disrupts Embryonic Neurogenesis and Causes p53-Mediated Microcephaly.
Mao H, McMahon JJ, Tsai YH, Wang Z, Silver DL
PLoS genetics12(9)e1006282(2016 09)

eIF4E3 acts as a tumor suppressor by utilizing an atypical mode of methyl-7-guanosine cap recognition.
Osborne MJ, Volpon L, Kornblatt JA, Culjkovic-Kraljacic B, Baguet A, Borden KL
Proceedings of the National Academy of Sciences of the United States of America110(10)3877-82(2013 Mar)

Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition.
Chen J, Melton C, Suh N, Oh JS, Horner K, Xie F, Sette C, Blelloch R, Conti M
Genes & development25(7)755-66(2011 Apr)

Systematic analysis of human protein complexes identifies chromosome segregation proteins.
Hutchins JR, Toyoda Y, Hegemann B, Poser I, Hériché JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM
Science (New York, N.Y.)328(5978)593-9(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Eif4a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif4a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10847	NM_138669.1 Latest version!	Mus musculus eukaryotic translation initiation factor 4A3 (Eif4a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif4a3 ( NM_138669.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_619610.1 Mus musculus eukaryotic translation initiation factor 4A3 (Eif4a3), mRNA.

Price & Availability↑

CloneID	OMu10847
Clone ID Related Accession (Same CDS sequence)	NM_138669.1
Accession Version	NM_138669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Mus musculus(house mouse)
Product	eukaryotic initiation factor 4A-III
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC008132.1. ##Evidence-Data-START## Transcript exon combination :: BC008132.1, AK150277.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCTA ACGCCACGAT GGCGACGTCT GGCTCGGCGC GGAAGCGGCT GCTCAAAGAG 
GAGGACATGA CCAAAGTGGA GTTCGAGACG AGCGAGGAGG TGGACGTGAC CCCCACGTTC 
GACACCATGG GCCTGCGAGA GGACCTGCTG CGCGGCATCT ACGCCTACGG TTTTGAAAAA 
CCTTCAGCGA TTCAGCAGCG TGCTATCAAG CAGATAATTA AAGGGAGAGA TGTCATTGCA 
CAGTCTCAGT CTGGCACAGG CAAGACGGCC ACCTTCAGTG TCTCAGTGCT GCAGTGCTTG 
GATATCCAGG TTCGAGAAAC CCAAGCTTTG ATCCTGGCTC CAACCAGGGA GTTAGCGGTG 
CAGATTCAGA AGGGTCTGCT CGCGCTGGGG GATTACATGA ACGTGCAGTG CCATGCCTGC 
ATTGGGGGCA CCAACGTCGG CGAGGACATC CGGAAGCTGG ACTACGGACA GCACGTGGTG 
GCAGGCACGC CGGGACGCGT CTTTGATATG ATCCGCCGGA GAAGTTTACG GACACGGGCT 
ATCAAGATGC TGGTTTTGGA TGAGGCTGAT GAAATGTTGA ACAAAGGTTT CAAGGAGCAG 
ATCTATGATG TGTACAGGTA CTTGCCACCA GCCACACAGG TCGTCCTCAT CAGCGCCACA 
CTGCCTCATG AGATCCTGGA GATGACCAAC AAGTTCATGA CCGACCCCAT CCGCATCTTG 
GTGAAGCGTG ATGAGTTGAC TCTGGAAGGC ATCAAACAGT TCTTTGTGGC TGTGGAAAGA 
GAGGAATGGA AATTTGATAC TCTATGTGAT CTCTATGACA CGCTGACCAT CACCCAGGCC 
GTCATCTTCT GCAACACCAA GCGGAAGGTT GACTGGCTGA CAGAGAAAAT GAGAGAAGCC 
AATTTCACTG TGTCGTCCAT GCATGGAGAC ATGCCCCAGA AAGAACGAGA GTCTATCATG 
AAGGAGTTCC GGTCAGGTGC CAGCCGGGTG CTCATCTCCA CAGACGTCTG GGCCCGGGGC 
CTGGATGTCC CTCAGGTGTC CCTCATCATT AACTACGACC TGCCCAACAA CAGAGAACTG 
TACATTCACA GAATTGGGAG ATCGGGTCGG TATGGACGAA AAGGTGTGGC CATCAATTTT 
GTGAAGAATG ATGACATCCG GATTCTCAGG GACATTGAGC AGTACTACTC CACCCAGATA 
GACGAGATGC CCATGAATGT GGCTGACCTC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619610.1
CDS	48..1283
Translation	MAANATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSVSVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI

Target ORF information:

RefSeq Version	NM_138669.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 4A3 (Eif4a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138669.1

ORF Insert Sequence:

ATGGCGGCTA ACGCCACGAT GGCGACGTCT GGCTCGGCGC GGAAGCGGCT GCTCAAAGAG 
GAGGACATGA CCAAAGTGGA GTTCGAGACG AGCGAGGAGG TGGACGTGAC CCCCACGTTC 
GACACCATGG GCCTGCGAGA GGACCTGCTG CGCGGCATCT ACGCCTACGG TTTTGAAAAA 
CCTTCAGCGA TTCAGCAGCG TGCTATCAAG CAGATAATTA AAGGGAGAGA TGTCATTGCA 
CAGTCTCAGT CTGGCACAGG CAAGACGGCC ACCTTCAGTG TCTCAGTGCT GCAGTGCTTG 
GATATCCAGG TTCGAGAAAC CCAAGCTTTG ATCCTGGCTC CAACCAGGGA GTTAGCGGTG 
CAGATTCAGA AGGGTCTGCT CGCGCTGGGG GATTACATGA ACGTGCAGTG CCATGCCTGC 
ATTGGGGGCA CCAACGTCGG CGAGGACATC CGGAAGCTGG ACTACGGACA GCACGTGGTG 
GCAGGCACGC CGGGACGCGT CTTTGATATG ATCCGCCGGA GAAGTTTACG GACACGGGCT 
ATCAAGATGC TGGTTTTGGA TGAGGCTGAT GAAATGTTGA ACAAAGGTTT CAAGGAGCAG 
ATCTATGATG TGTACAGGTA CTTGCCACCA GCCACACAGG TCGTCCTCAT CAGCGCCACA 
CTGCCTCATG AGATCCTGGA GATGACCAAC AAGTTCATGA CCGACCCCAT CCGCATCTTG 
GTGAAGCGTG ATGAGTTGAC TCTGGAAGGC ATCAAACAGT TCTTTGTGGC TGTGGAAAGA 
GAGGAATGGA AATTTGATAC TCTATGTGAT CTCTATGACA CGCTGACCAT CACCCAGGCC 
GTCATCTTCT GCAACACCAA GCGGAAGGTT GACTGGCTGA CAGAGAAAAT GAGAGAAGCC 
AATTTCACTG TGTCGTCCAT GCATGGAGAC ATGCCCCAGA AAGAACGAGA GTCTATCATG 
AAGGAGTTCC GGTCAGGTGC CAGCCGGGTG CTCATCTCCA CAGACGTCTG GGCCCGGGGC 
CTGGATGTCC CTCAGGTGTC CCTCATCATT AACTACGACC TGCCCAACAA CAGAGAACTG 
TACATTCACA GAATTGGGAG ATCGGGTCGG TATGGACGAA AAGGTGTGGC CATCAATTTT 
GTGAAGAATG ATGACATCCG GATTCTCAGG GACATTGAGC AGTACTACTC CACCCAGATA 
GACGAGATGC CCATGAATGT GGCTGACCTC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EIF4A3 deficient human iPSCs and mouse models demonstrate neural crest defects that underlie Richieri-Costa-Pereira syndrome.
Human molecular genetics26(12)2177-2191(2017 06)
Miller EE,Kobayashi GS,Musso CM,Allen M,Ishiy FAA,de Caires LC Jr,Goulart E,Griesi-Oliveira K,Zechi-Ceide RM,Richieri-Costa A,Bertola DR,Passos-Bueno MR,Silver DL

Haploinsufficiency for Core Exon Junction Complex Components Disrupts Embryonic Neurogenesis and Causes p53-Mediated Microcephaly.
PLoS genetics12(9)e1006282(2016 09)
Mao H,McMahon JJ,Tsai YH,Wang Z,Silver DL

eIF4E3 acts as a tumor suppressor by utilizing an atypical mode of methyl-7-guanosine cap recognition.
Proceedings of the National Academy of Sciences of the United States of America110(10)3877-82(2013 Mar)
Osborne MJ,Volpon L,Kornblatt JA,Culjkovic-Kraljacic B,Baguet A,Borden KL

Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition.
Genes & development25(7)755-66(2011 Apr)
Chen J,Melton C,Suh N,Oh JS,Horner K,Xie F,Sette C,Blelloch R,Conti M

Systematic analysis of human protein complexes identifies chromosome segregation proteins.
Science (New York, N.Y.)328(5978)593-9(2010 Apr)
Hutchins JR,Toyoda Y,Hegemann B,Poser I,Hériché JK,Sykora MM,Augsburg M,Hudecz O,Buschhorn BA,Bulkescher J,Conrad C,Comartin D,Schleiffer A,Sarov M,Pozniakovsky A,Slabicki MM,Schloissnig S,Steinmacher I,Leuschner M,Ssykor A,Lawo S,Pelletier L,Stark H,Nasmyth K,Ellenberg J,Durbin R,Buchholz F,Mechtler K,Hyman AA,Peters JM

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Eif4a3 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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