Pola1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pola1
Entrez Gene ID	18968
Full Name	polymerase (DNA directed), alpha 1
Synonyms	AW321876,Pola
General protein information	Preferred Names polymerase (DNA directed), alpha 1 Names DNA polymerase alpha catalytic subunit DNA polymerase alpha 1, 180 kDa DNA polymerase alpha catalytic subunit p180 G7a/Bat6Hom polymerase, alpha 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X C3\|X 41.06 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006527905.4	XP_006527968.1	DNA polymerase alpha catalytic subunit isoform X1
NM_008892.2	NP_032918.1	DNA polymerase alpha catalytic subunit
XM_006527905.3	XP_006527968.1	DNA polymerase alpha catalytic subunit isoform X1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AER381C	NP_985237.1
Macaca mulatta (Rhesus monkey)	POLA1	XP_001091195.1
Gallus gallus (chicken)	POLA1	XP_416792.3
Homo sapiens (human)	POLA1	NP_058633.2
Rattus norvegicus (Norway rat)	Pola1	NP_445931.1
Schizosaccharomyces pombe (fission yeast)	pol1	NP_594186.1
Neurospora crassa	NCU07870	XP_963131.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001929	XP_321134.5
Magnaporthe oryzae (rice blast fungus)	MGG_06397	XP_003717174.1
Mus musculus (house mouse)	Pola1	NP_032918.1
Danio rerio (zebrafish)	pola1	XP_005162809.1
Drosophila melanogaster (fruit fly)	DNApol-alpha180	NP_536736.2
Caenorhabditis elegans (roundworm)	Y47D3A.29	NP_001255109.1
Arabidopsis thaliana (thale cress)	ICU2	NP_201511.2
Xenopus tropicalis (tropical clawed frog)	pola1	XP_004911812.1
Pan troglodytes (chimpanzee)	POLA1	XP_003317445.2
Kluyveromyces lactis	KLLA0C13255g	XP_452791.1
Bos taurus (cattle)	POLA1	NP_001192994.1
Saccharomyces cerevisiae (baker's yeast)	POL1	NP_014297.3
Canis lupus familiaris (dog)	POLA1	XP_005641271.1
Oryza sativa (rice)	Os01g0868300	NP_001044920.1

Gene Ontology
Bibliography

Related articles in PubMed

Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis.
Bellelli R, Borel V, Logan C, Svendsen J, Cox DE, Nye E, Metcalfe K, O'Connell SM, Stamp G, Flynn HR, Snijders AP, Lassailly F, Jackson A, Boulton SJ
Molecular cell70(4)707-721.e7(2018 05)

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Ray JP, Staron MM, Shyer JA, Ho PC, Marshall HD, Gray SM, Laidlaw BJ, Araki K, Ahmed R, Kaech SM, Craft J
Immunity43(4)690-702(2015 Oct)

Phenotypic annotation of the mouse X chromosome.
Cox BJ, Vollmer M, Tamplin O, Lu M, Biechele S, Gertsenstein M, van Campenhout C, Floss T, Kühn R, Wurst W, Lickert H, Rossant J
Genome research20(8)1154-64(2010 Aug)

Aberrant DNA polymerase alpha is excluded from the nucleus by defective import and degradation in the nucleus.
Eichinger CS, Mizuno T, Mizuno K, Miyake Y, Yanagi K, Imamoto N, Hanaoka F
The Journal of biological chemistry284(44)30604-14(2009 Oct)

Anti-tumor effects of dehydroaltenusin, a specific inhibitor of mammalian DNA polymerase alpha.
Maeda N, Kokai Y, Ohtani S, Sahara H, Kuriyama I, Kamisuki S, Takahashi S, Sakaguchi K, Sugawara F, Yoshida H, Sato N, Mizushina Y
Biochemical and biophysical research communications352(2)390-6(2007 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pola1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pola1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15413	NM_008892.2 Latest version!	Mus musculus polymerase (DNA directed), alpha 1 (Pola1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu56208	XM_006527905.4 Latest version!	Mus musculus polymerase (DNA directed), alpha 1 (Pola1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OMu56208	XM_006527905.3	Mus musculus polymerase (DNA directed), alpha 1 (Pola1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pola1 ( NM_008892.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032918.1 Mus musculus polymerase (DNA directed), alpha 1 (Pola1), mRNA.

Price & Availability↑

CloneID	OMu15413
Clone ID Related Accession (Same CDS sequence)	NM_008892.2
Accession Version	NM_008892.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Mus musculus(house mouse)
Product	DNA polymerase alpha catalytic subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from D17384.1, BC138047.1 and CJ133126.1. On Oct 31, 2009 this sequence version replaced NM_008892.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D17384.1, D13543.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGCCCA TGCACGAAGA GGACTGTAAA CTGGAGGCAA GCGCTGTGTC AGATTCAGGG 
AGTTTTGCAG CTTCCCGAGC TCGGCGAGAA AAGAAATCAA AGAAAGGACG TCAAGAAGCT 
TTAGAGAGAC TGAAAAAGGC TAAAGCTGGG GAGAAGTATA AATACGAAGT TGAGGACCTC 
ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCGA AACTGGTTCA GGCCCGTCAG 
GATGATGACT GGATTGTGGA TGACGATGGG ATAGGCTATG TGGAAGATGG CAGAGAGATT 
TTTGATGATG ACCTTGAGGA TGATGCACTT GACACCTGTG GAAAAGGAAG TGATGGCAAA 
GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC 
AAGGCAATGT TCATTGCTAG TGCCGGGAAG AAAACTACAG ATAAAGCAGT AGACTTGTCC 
AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTAAACA CTGAGACAGC TCAAATAACT 
CCACCACCTG TATTGATACC AAAGAAAAAA AGATCTACTG GGGCTTTGCT CAACCCTTTT 
TCAGTGCACA CCCCTAAGGC AATTCCTTCA GGAAAACCTG CTTCCCCTGT CCTCAGGAAT 
GAGCCTCTGT TAACTCCCAT TCCTCTTAAA CGTGCTGAAC TTGCTGGAGA GCTGGCTCAG 
CCAGAATGTC CAGAAGATGA GCAGGAGCTA GGGGTAATGG AGTTTGAAGA CGGTGACTTT 
GATGAGTCCA TGGACACAGA GAAGGTAGAT GAGAAGCCTG TGACTGCTAA GACTTGGGAC 
CAAGAGACTG AGCCAGTAGA AAGGGTGGAA CATGAGGCAG ATCCTGAGAG GGGAACCACA 
TCTTACTTAG AAAATTTTCT GCCAGATGTC TCTTGTTGGG ACATTGATCA GGATGATGAA 
AGCATACCTC AGGAAGTTCA AGTGGATTCC AGTAATCTCC CATTGGTAAA AGGGGCAGAT 
GATGAACAAG TGTTTCAGTT TTATTGGCTG GATGCTTATG AAGATCCATA CAACCAACCA 
GGTGTGGTTT TTCTGTTTGG CAAAGTGTGG ATTGAATCAG TCAAAACCCA TGTGAGCTGT 
TGTGTTATGG TGAAAAATAT TGAGCGAACA CTCTACTTCC TTCCCCGTGA AATGAAATTT 
GACCTAAACA CAGGGAAAGA AACAGCAATT CCAGTTACAA TGAAGGATGT TTATGAAGAA 
TTTGATTCAA AAATTTCAGC AAAATATAAA ATAATGAAGT TCAAGTCCAA GATAGTGGAA 
AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACCT GGAAGTTAGA 
TACTCGGCAG AAGTGCCACA GCTTCCACAG AATTTAAAAG GAGAAACATT TTCTCATGTG 
TTTGGAACTA ACACGTCTAG CTTGGAACTG TTCTTGATGA ATAGGAAGAT CAAAGGACCT 
TGTTGGCTTG AAGTAAAAAA TCCACAACTC TTGAATCAGC CAATCAGTTG GTGTAAATTT 
GAGGTAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCTCCTCCA 
CTTGTGGTGA TGTCTTTCAG CATGAAGACA ATGCAGAATG TACAGAACCA TCAACATGAG 
ATTATTGCTA TGGCGGCTTT GGTTCATCAC AGTTTTGCAT TAGATAAAGC ACCTCCAGAG 
CCTCCCTTTC AGACACATTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTCCCATGT 
GATTTCAAAG AAGTGATTTC GAAAAAGAAT ATGAAGGTTG AGATCGCTGC AACAGAAAGA 
ACCTTGATAG GATTTTTCCT TGCAAAAGTT CACAAAATTG ACCCTGACAT TCTTGTGGGT 
CATAATATTT GTAGTTTTGA ACTGGAAGTG CTGCTGCAAA GAATTAATGA GTGCAAGGTT 
CCTTACTGGT CCAAGATAGG TCGGCTGAGG AGATCCAACA TGCCAAAACT TGGGAGCCGG 
AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGCCGAATGA TATGTGATGT GGAAATTTCA 
GCAAAAGAAT TGATTCATTG TAAAAGCTAC CATTTGTCTG AACTTGTTCA GCAGATTCTG 
AAAACTGAGA GGATTGTAAT TCCAACAGAA AATATACGAA ACATGTATAG TGAATCTTCT 
TATCTGTTGT ACCTGTTGGA ACATATCTGG AAAGATGCCA GATTCATTTT GCAGATCATG 
TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG 
TCTAGGACAC TGATGGGTGG ACGATCTGAG CGAAATGAGT TTTTGTTGCT TCATGCATTT 
TATGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAACCTCA GCAAAAACTG 
GGAGATGAAG ATGAGGAAAT TGATGGGGAC ACCAATAAAT ACAAGAAAGG GCGTAAGAAA 
GCAACTTACG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA CAAGTTCATT 
TTGCTCCTGG ATTTCAACAG TTTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT 
ACAACAGTGC AAAGAGTTAC TTCAGAGGTG CAGAAAGCTA CAGAGGATGA GGAACAAGAA 
CAGATCCCTG AGTTGCCAGA TCCAAACTTA GAGATGGGCA TTTTGCCCAG AGAGATTCGG 
AAACTGGTAG AGCGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA TTTAAATCCA 
GACCTTGTTC TTCAGTATGA CATCCGACAG AAGGCTTTGA AGCTCACAGC AAACAGTATG 
TATGGTTGCC TGGGATTTTC GTACAGCAGA TTTTATGCTA AACCACTGGC TGCCTTGGTG 
ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGACA TGGTACAGAA GATGAATCTT 
GAAGTTATTT ATGGAGACAC AGATTCAATT ATGATAAATA CCAATAGCAC CAACCTGGAA 
GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAATTGTA CAAACTGCTT 
GAAATAGACA TTGATGCTGT TTTCAAGTCT CTGCTGTTGC TGAAGAAAAA GAAATATGCT 
GCTCTAGTTG TTGAACCAAC ATCAGATGGG AATTACATTA CCAAGCAGGA GCTGAAAGGA 
TTAGATATAG TTAGAAGAGA CTGGTGTGAT CTTGCCAAGG ACACTGGAAA CTTTGTCATT 
GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACCATTGTGG AAAACATTCA GAAGAGGTTA 
ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC 
AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAGAA AAAGCTTACC TCATGTACAT 
GTTGCACTGT GGATAAATTC TCAAGGAGGC AGGAAGGTGA AAGCTGGTGA TACGGTGTCC 
TATGTTATTT GTCAGGATGG GTCAAACCTC ACTGCAACTC AGAGGGCCTA TGCCCCAGAA 
CAATTGCAGA AACTTGACAA TTTAGCCATC GACACCCAAT ACTACTTGGC CCAGCAGATC 
CACCCTGTTG TGGCTCGGAT CTGTGAGCCA ATAGATGGAA TTGATGCTGT ACTCATTGCA 
TTATGGCTAG GACTTGACTC CACTCAATTT AGAGTTCATC AGTATCACAA AGATGAAGAG 
AATGATGCCC TCCTTGGTGG TCCAGCACAG CTAACTGATG AAGAGAAATA CAAGGATTGT 
GAAAAATTCA AATGCCTTTG TCCTTCATGT GGAACTGAAA ATATTTATGA TAATGTCTTT 
GAAGGCTCGG GACTGGATAT GGAACCCAGT TTGTATCGTT GCAGTAATGT TGATTGTAAG 
GTTTCACCGT TGACCTTCAT GGTACAGCTG AGCAACAAAT TGATCATGGA CATTAGACGG 
TGCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACTTG TTGCAGTCGC 
CTTCGACGTC TTCCCCTTCA TTTCTCCCGA AATGGGCCTC TGTGTCCAGT CTGCATGAAA 
GCTGTGCTTA GACCAGAGTA TTCTGACAAG TCCTTGTACA CCCAGCTGTG CTTTTACCGG 
TACATTTTTG ATGCGGACTG TGCACTGGAA AAACTTACTG AACATGAGAA AGATAAATTG 
AAGAAGCAGT TTTTCCCTCT CCGAGTTCTT CAGGACTACA GAAAAGTCAA GAACATAGCA 
GAGCAGTTCT TGTCCTGGAG TGGCTACTCT GAAGTGAACC TCAGCAAGCT CTTCGCCAAT 
TACGCTGGGA AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032918.1
CDS	45..4442
Translation	MAPMHEEDCKLEASAVSDSGSFAASRARREKKSKKGRQEALERLKKAKAGEKYKYEVEDLTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDTCGKGSDGKAHRKDRKDVKKPSVTKPNNIKAMFIASAGKKTTDKAVDLSKDDLLGDILQDLNTETAQITPPPVLIPKKKRSTGALLNPFSVHTPKAIPSGKPASPVLRNEPLLTPIPLKRAELAGELAQPECPEDEQELGVMEFEDGDFDESMDTEKVDEKPVTAKTWDQETEPVERVEHEADPERGTTSYLENFLPDVSCWDIDQDDESIPQEVQVDSSNLPLVKGADDEQVFQFYWLDAYEDPYNQPGVVFLFGKVWIESVKTHVSCCVMVKNIERTLYFLPREMKFDLNTGKETAIPVTMKDVYEEFDSKISAKYKIMKFKSKIVEKNYAFEIPDVPEKSEYLEVRYSAEVPQLPQNLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKFEVMALKPDLVNVIKDVSPPPLVVMSFSMKTMQNVQNHQHEIIAMAALVHHSFALDKAPPEPPFQTHFCVVSKPKDCIFPCDFKEVISKKNMKVEIAATERTLIGFFLAKVHKIDPDILVGHNICSFELEVLLQRINECKVPYWSKIGRLRRSNMPKLGSRSGFGERNATCGRMICDVEISAKELIHCKSYHLSELVQQILKTERIVIPTENIRNMYSESSYLLYLLEHIWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKKATYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVTSEVQKATEDEEQEQIPELPDPNLEMGILPREIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKDMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDAVFKSLLLLKKKKYAALVVEPTSDGNYITKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDRKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTATQRAYAPEQLQKLDNLAIDTQYYLAQQIHPVVARICEPIDGIDAVLIALWLGLDSTQFRVHQYHKDEENDALLGGPAQLTDEEKYKDCEKFKCLCPSCGTENIYDNVFEGSGLDMEPSLYRCSNVDCKVSPLTFMVQLSNKLIMDIRRCIKKYYDGWLICEEPTCCSRLRRLPLHFSRNGPLCPVCMKAVLRPEYSDKSLYTQLCFYRYIFDADCALEKLTEHEKDKLKKQFFPLRVLQDYRKVKNIAEQFLSWSGYSEVNLSKLFANYAGKS

Target ORF information:

RefSeq Version	NM_008892.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), alpha 1 (Pola1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008892.2

ORF Insert Sequence:

ATGGCGCCCA TGCACGAAGA GGACTGTAAA CTGGAGGCAA GCGCTGTGTC AGATTCAGGG 
AGTTTTGCAG CTTCCCGAGC TCGGCGAGAA AAGAAATCAA AGAAAGGACG TCAAGAAGCT 
TTAGAGAGAC TGAAAAAGGC TAAAGCTGGG GAGAAGTATA AATACGAAGT TGAGGACCTC 
ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCGA AACTGGTTCA GGCCCGTCAG 
GATGATGACT GGATTGTGGA TGACGATGGG ATAGGCTATG TGGAAGATGG CAGAGAGATT 
TTTGATGATG ACCTTGAGGA TGATGCACTT GACACCTGTG GAAAAGGAAG TGATGGCAAA 
GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC 
AAGGCAATGT TCATTGCTAG TGCCGGGAAG AAAACTACAG ATAAAGCAGT AGACTTGTCC 
AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTAAACA CTGAGACAGC TCAAATAACT 
CCACCACCTG TATTGATACC AAAGAAAAAA AGATCTACTG GGGCTTTGCT CAACCCTTTT 
TCAGTGCACA CCCCTAAGGC AATTCCTTCA GGAAAACCTG CTTCCCCTGT CCTCAGGAAT 
GAGCCTCTGT TAACTCCCAT TCCTCTTAAA CGTGCTGAAC TTGCTGGAGA GCTGGCTCAG 
CCAGAATGTC CAGAAGATGA GCAGGAGCTA GGGGTAATGG AGTTTGAAGA CGGTGACTTT 
GATGAGTCCA TGGACACAGA GAAGGTAGAT GAGAAGCCTG TGACTGCTAA GACTTGGGAC 
CAAGAGACTG AGCCAGTAGA AAGGGTGGAA CATGAGGCAG ATCCTGAGAG GGGAACCACA 
TCTTACTTAG AAAATTTTCT GCCAGATGTC TCTTGTTGGG ACATTGATCA GGATGATGAA 
AGCATACCTC AGGAAGTTCA AGTGGATTCC AGTAATCTCC CATTGGTAAA AGGGGCAGAT 
GATGAACAAG TGTTTCAGTT TTATTGGCTG GATGCTTATG AAGATCCATA CAACCAACCA 
GGTGTGGTTT TTCTGTTTGG CAAAGTGTGG ATTGAATCAG TCAAAACCCA TGTGAGCTGT 
TGTGTTATGG TGAAAAATAT TGAGCGAACA CTCTACTTCC TTCCCCGTGA AATGAAATTT 
GACCTAAACA CAGGGAAAGA AACAGCAATT CCAGTTACAA TGAAGGATGT TTATGAAGAA 
TTTGATTCAA AAATTTCAGC AAAATATAAA ATAATGAAGT TCAAGTCCAA GATAGTGGAA 
AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACCT GGAAGTTAGA 
TACTCGGCAG AAGTGCCACA GCTTCCACAG AATTTAAAAG GAGAAACATT TTCTCATGTG 
TTTGGAACTA ACACGTCTAG CTTGGAACTG TTCTTGATGA ATAGGAAGAT CAAAGGACCT 
TGTTGGCTTG AAGTAAAAAA TCCACAACTC TTGAATCAGC CAATCAGTTG GTGTAAATTT 
GAGGTAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCTCCTCCA 
CTTGTGGTGA TGTCTTTCAG CATGAAGACA ATGCAGAATG TACAGAACCA TCAACATGAG 
ATTATTGCTA TGGCGGCTTT GGTTCATCAC AGTTTTGCAT TAGATAAAGC ACCTCCAGAG 
CCTCCCTTTC AGACACATTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTCCCATGT 
GATTTCAAAG AAGTGATTTC GAAAAAGAAT ATGAAGGTTG AGATCGCTGC AACAGAAAGA 
ACCTTGATAG GATTTTTCCT TGCAAAAGTT CACAAAATTG ACCCTGACAT TCTTGTGGGT 
CATAATATTT GTAGTTTTGA ACTGGAAGTG CTGCTGCAAA GAATTAATGA GTGCAAGGTT 
CCTTACTGGT CCAAGATAGG TCGGCTGAGG AGATCCAACA TGCCAAAACT TGGGAGCCGG 
AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGCCGAATGA TATGTGATGT GGAAATTTCA 
GCAAAAGAAT TGATTCATTG TAAAAGCTAC CATTTGTCTG AACTTGTTCA GCAGATTCTG 
AAAACTGAGA GGATTGTAAT TCCAACAGAA AATATACGAA ACATGTATAG TGAATCTTCT 
TATCTGTTGT ACCTGTTGGA ACATATCTGG AAAGATGCCA GATTCATTTT GCAGATCATG 
TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG 
TCTAGGACAC TGATGGGTGG ACGATCTGAG CGAAATGAGT TTTTGTTGCT TCATGCATTT 
TATGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAACCTCA GCAAAAACTG 
GGAGATGAAG ATGAGGAAAT TGATGGGGAC ACCAATAAAT ACAAGAAAGG GCGTAAGAAA 
GCAACTTACG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA CAAGTTCATT 
TTGCTCCTGG ATTTCAACAG TTTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT 
ACAACAGTGC AAAGAGTTAC TTCAGAGGTG CAGAAAGCTA CAGAGGATGA GGAACAAGAA 
CAGATCCCTG AGTTGCCAGA TCCAAACTTA GAGATGGGCA TTTTGCCCAG AGAGATTCGG 
AAACTGGTAG AGCGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA TTTAAATCCA 
GACCTTGTTC TTCAGTATGA CATCCGACAG AAGGCTTTGA AGCTCACAGC AAACAGTATG 
TATGGTTGCC TGGGATTTTC GTACAGCAGA TTTTATGCTA AACCACTGGC TGCCTTGGTG 
ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGACA TGGTACAGAA GATGAATCTT 
GAAGTTATTT ATGGAGACAC AGATTCAATT ATGATAAATA CCAATAGCAC CAACCTGGAA 
GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAATTGTA CAAACTGCTT 
GAAATAGACA TTGATGCTGT TTTCAAGTCT CTGCTGTTGC TGAAGAAAAA GAAATATGCT 
GCTCTAGTTG TTGAACCAAC ATCAGATGGG AATTACATTA CCAAGCAGGA GCTGAAAGGA 
TTAGATATAG TTAGAAGAGA CTGGTGTGAT CTTGCCAAGG ACACTGGAAA CTTTGTCATT 
GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACCATTGTGG AAAACATTCA GAAGAGGTTA 
ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC 
AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAGAA AAAGCTTACC TCATGTACAT 
GTTGCACTGT GGATAAATTC TCAAGGAGGC AGGAAGGTGA AAGCTGGTGA TACGGTGTCC 
TATGTTATTT GTCAGGATGG GTCAAACCTC ACTGCAACTC AGAGGGCCTA TGCCCCAGAA 
CAATTGCAGA AACTTGACAA TTTAGCCATC GACACCCAAT ACTACTTGGC CCAGCAGATC 
CACCCTGTTG TGGCTCGGAT CTGTGAGCCA ATAGATGGAA TTGATGCTGT ACTCATTGCA 
TTATGGCTAG GACTTGACTC CACTCAATTT AGAGTTCATC AGTATCACAA AGATGAAGAG 
AATGATGCCC TCCTTGGTGG TCCAGCACAG CTAACTGATG AAGAGAAATA CAAGGATTGT 
GAAAAATTCA AATGCCTTTG TCCTTCATGT GGAACTGAAA ATATTTATGA TAATGTCTTT 
GAAGGCTCGG GACTGGATAT GGAACCCAGT TTGTATCGTT GCAGTAATGT TGATTGTAAG 
GTTTCACCGT TGACCTTCAT GGTACAGCTG AGCAACAAAT TGATCATGGA CATTAGACGG 
TGCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACTTG TTGCAGTCGC 
CTTCGACGTC TTCCCCTTCA TTTCTCCCGA AATGGGCCTC TGTGTCCAGT CTGCATGAAA 
GCTGTGCTTA GACCAGAGTA TTCTGACAAG TCCTTGTACA CCCAGCTGTG CTTTTACCGG 
TACATTTTTG ATGCGGACTG TGCACTGGAA AAACTTACTG AACATGAGAA AGATAAATTG 
AAGAAGCAGT TTTTCCCTCT CCGAGTTCTT CAGGACTACA GAAAAGTCAA GAACATAGCA 
GAGCAGTTCT TGTCCTGGAG TGGCTACTCT GAAGTGAACC TCAGCAAGCT CTTCGCCAAT 
TACGCTGGGA AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pola1 ( XM_006527905.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527968.1 Mus musculus polymerase (DNA directed), alpha 1 (Pola1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu56208
Clone ID Related Accession (Same CDS sequence)	XM_006527905.4 , XM_006527905.3
Accession Version	XM_006527905.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	DNA polymerase alpha catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527905.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCA TGCACGAAGA GGACTGTAAA CTGGAGGCAA GCGCTGTGTC AGATTCAGGG 
AGTTTTGCAG CTTCCCGAGC TCGGCGAGAA AAGAAATCAA AGAAAGGACG TCAAGAAGCT 
TTAGAGAGAC TGAAAAAGGC TAAAGCTGGG GAGAAGTATA AATACGAAGT TGAGGACCTC 
ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCGA AACTGGTTCA GGCCCGTCAG 
GATGATGACT GGATTGTGGA TGACGATGGG ATAGGCTATG TGGAAGATGG CAGAGAGATT 
TTTGATGATG ACCTTGAGGA TGATGCACTT GACACCTGTG GAAAAGGAAG TGATGGCAAA 
GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC 
AAGGCAATGT TCATTGCTAG TGCCGGGAAG AAAACTACAG ATAAAGCAGT AGACTTGTCC 
AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTAAACA CTGAGACAGC TCAAATAACT 
CCACCACCTG TATTGATACC AAAGAAAAAA AGATCTACTG GGGCTTTGCT CAACCCTTTT 
TCAGTGCACA CCCCTAAGGC AATTCCTTCA GGAAAACCTG CTTCCCCTGT CCTCAGGAAT 
GAGCCTCTGT TAACTCCCAT TCCTCTTAAA CGTGCTGAAC TTGCTGGAGA GCTGGCTCAG 
CCAGAATGTC CAGAAGATGA GCAGGAGCTA GGGGTAATGG AGTTTGAAGA CGGTGACTTT 
GATGAGTCCA TGGACACAGA GAAGGTAGAT GAGAAGCCTG TGACTGCTAA GACTTGGGAC 
CAAGAGACTG AGCCAGTAGA AAGGGTGGAA CATGAGGCAG ATCCTGAGAG GGGAACCACA 
TCTTACTTAG AAAATTTTCT GCCAGATGTC TCTTGTTGGG ACATTGATCA GGATGATGAA 
AGCATACCTC AGGAAGTTCA AGTGGATTCC AGTAATCTCC CATTGGTAAA AGGGGCAGAT 
GATGAACAAG TGTTTCAGTT TTATTGGCTG GATGCTTATG AAGATCCATA CAACCAACCA 
GGTGTGGTTT TTCTGTTTGG CAAAGTGTGG ATTGAATCAG TCAAAACCCA TGTGAGCTGT 
TGTGTTATGG TGAAAAATAT TGAGCGAACA CTCTACTTCC TTCCCCGTGA AATGAAATTT 
GACCTAAACA CAGGGAAAGA AACAGCAATT CCAGTTACAA TGAAGGATGT TTATGAAGAA 
TTTGATTCAA AAATTTCAGC AAAATATAAA ATAATGAAGT TCAAGTCCAA GATAGTGGAA 
AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACCT GGAAGTTAGA 
TACTCGGCAG AAGTGCCACA GCTTCCACAG AATTTAAAAG GAGAAACATT TTCTCATGTG 
TTTGGAACTA ACACGTCTAG CTTGGAACTG TTCTTGATGA ATAGGAAGAT CAAAGGACCT 
TGTTGGCTTG AAGTAAAAAA TCCACAACTC TTGAATCAGC CAATCAGTTG GTGTAAATTT 
GAGGTAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCTCCTCCA 
CTTGTGGTGA TGTCTTTCAG CATGAAGACA ATGCAGAATG TACAGAACCA TCAACATGAG 
ATTATTGCTA TGGCGGCTTT GGTTCATCAC AGTTTTGCAT TAGATAAAGC ACCTCCAGAG 
CCTCCCTTTC AGACACATTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTCCCATGT 
GATTTCAAAG AAGTGATTTC GAAAAAGAAT ATGAAGGTTG AGATCGCTGC AACAGAAAGA 
ACCTTGATAG GATTTTTCCT TGCAAAAGTT CACAAAATTG ACCCTGACAT TCTTGTGGGT 
CATAATATTT GTAGTTTTGA ACTGGAAGTG CTGCTGCAAA GAATTAATGA GTGCAAGGTT 
CCTTACTGGT CCAAGATAGG TCGGCTGAGG AGATCCAACA TGCCAAAACT TGGGAGCCGG 
AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGCCGAATGA TATGTGATGT GGAAATTTCA 
GCAAAAGAAT TGATTCATTG TAAAAGCTAC CATTTGTCTG AACTTGTTCA GCAGATTCTG 
AAAACTGAGA GGATTGTAAT TCCAACAGAA AATATACGAA ACATGTATAG TGAATCTTCT 
TATCTGTTGT ACCTGTTGGA ACATATCTGG AAAGATGCCA GATTCATTTT GCAGATCATG 
TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG 
TCTAGGACAC TGATGGGTGG ACGATCTGAG CGAAATGAGT TTTTGTTGCT TCATGCATTT 
TATGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAACCTCA GCAAAAACTG 
GGAGATGAAG ATGAGGAAAT TGATGGGGAC ACCAATAAAT ACAAGAAAGG GCGTAAGAAA 
GCAACTTACG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA CAAGTTCATT 
TTGCTCCTGG ATTTCAACAG TTTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT 
ACAACAGTGC AAAGAGTTAC TTCAGAGGTG CAGAAAGCTA CAGAGGATGA GGAACAAGAA 
CAGATCCCTG AGTTGCCAGA TCCAAACTTA GAGATGGGCA TTTTGCCCAG AGAGATTCGG 
AAACTGGTAG AGCGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA TTTAAATCCA 
GACCTTGTTC TTCAGTATGA CATCCGACAG AAGGCTTTGA AGCTCACAGC AAACAGTATG 
TATGGTTGCC TGGGATTTTC GTACAGCAGA TTTTATGCTA AACCACTGGC TGCCTTGGTG 
ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGACA TGGTACAGAA GATGAATCTT 
GAAGTTATTT ATGGAGACAC AGATTCAATT ATGATAAATA CCAATAGCAC CAACCTGGAA 
GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAATTGTA CAAACTGCTT 
GAAATAGACA TTGATGCTGT TTTCAAGTCT CTGCTGTTGC TGAAGAAAAA GAAATATGCT 
GCTCTAGTTG TTGAACCAAC ATCAGATGGG AATTACATTA CCAAGCAGGA GCTGAAAGGA 
TTAGATATAG TTAGAAGAGA CTGGTGTGAT CTTGCCAAGG ACACTGGAAA CTTTGTCATT 
GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACCATTGTGG AAAACATTCA GAAGAGGTTA 
ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC 
AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAGAA AAAGCTTACC TCATGTACAT 
GTTGCACTGT GGATAAATTC TCAAGGAGGC AGGAAGGTGA AAGCTGGTGA TACGGTGTCC 
TATGTTATTT GTCAGGATGG GTCAAACCTC ACTGCAACTC AGAGGGCCTA TGCCCCAGAA 
CAATTGCAGA AACTTGACAA TTTAGCCATC GACACCCAAT ACTACTTGGC CCAGCAGATC 
CACCCTGTTG TGGCTCGGAT CTGTGAGCCA ATAGATGGAA TTGATGCTGT ACTCATTGCA 
TTATGGCTAG GACTTGACTC CACTCAATTT AGAGTTCATC AGTATCACAA AGATGAAGAG 
AATGATGCCC TCCTTGGTGG TCCAGCACAG CTAACTGATG AAGAGAAATA CAAGGATTGT 
GAAAAATTCA AATGCCTTTG TCCTTCATGT GGAACTGAAA ATATTTATGA TAATGTCTTT 
GAAGGCTCGG GACTGGATAT GGAACCCAGT TTGTATCGTT GCAGTAATGT TGATTGTAAG 
GTTTCACCGT TGACCTTCAT GGTACAGCTG AGCAACAAAT TGATCATGGA CATTAGACGG 
TGCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACTTG TTGCAGTCGC 
CTTCGACGTC TTCCCCTTCA TTTCTCCCGA AATGGGCCTC TGTGTCCAGT CTGCATGAAA 
GCTGTGCTTA GACCAGAGAT AAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527968.1
CDS	277..4443
Translation	MAPMHEEDCKLEASAVSDSGSFAASRARREKKSKKGRQEALERLKKAKAGEKYKYEVEDLTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDTCGKGSDGKAHRKDRKDVKKPSVTKPNNIKAMFIASAGKKTTDKAVDLSKDDLLGDILQDLNTETAQITPPPVLIPKKKRSTGALLNPFSVHTPKAIPSGKPASPVLRNEPLLTPIPLKRAELAGELAQPECPEDEQELGVMEFEDGDFDESMDTEKVDEKPVTAKTWDQETEPVERVEHEADPERGTTSYLENFLPDVSCWDIDQDDESIPQEVQVDSSNLPLVKGADDEQVFQFYWLDAYEDPYNQPGVVFLFGKVWIESVKTHVSCCVMVKNIERTLYFLPREMKFDLNTGKETAIPVTMKDVYEEFDSKISAKYKIMKFKSKIVEKNYAFEIPDVPEKSEYLEVRYSAEVPQLPQNLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKFEVMALKPDLVNVIKDVSPPPLVVMSFSMKTMQNVQNHQHEIIAMAALVHHSFALDKAPPEPPFQTHFCVVSKPKDCIFPCDFKEVISKKNMKVEIAATERTLIGFFLAKVHKIDPDILVGHNICSFELEVLLQRINECKVPYWSKIGRLRRSNMPKLGSRSGFGERNATCGRMICDVEISAKELIHCKSYHLSELVQQILKTERIVIPTENIRNMYSESSYLLYLLEHIWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKKATYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVTSEVQKATEDEEQEQIPELPDPNLEMGILPREIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKDMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDAVFKSLLLLKKKKYAALVVEPTSDGNYITKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDRKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTATQRAYAPEQLQKLDNLAIDTQYYLAQQIHPVVARICEPIDGIDAVLIALWLGLDSTQFRVHQYHKDEENDALLGGPAQLTDEEKYKDCEKFKCLCPSCGTENIYDNVFEGSGLDMEPSLYRCSNVDCKVSPLTFMVQLSNKLIMDIRRCIKKYYDGWLICEEPTCCSRLRRLPLHFSRNGPLCPVCMKAVLRPEIN

Target ORF information:

RefSeq Version	XM_006527905.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), alpha 1 (Pola1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527905.4

ORF Insert Sequence:

ATGGCGCCCA TGCACGAAGA GGACTGTAAA CTGGAGGCAA GCGCTGTGTC AGATTCAGGG 
AGTTTTGCAG CTTCCCGAGC TCGGCGAGAA AAGAAATCAA AGAAAGGACG TCAAGAAGCT 
TTAGAGAGAC TGAAAAAGGC TAAAGCTGGG GAGAAGTATA AATACGAAGT TGAGGACCTC 
ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCGA AACTGGTTCA GGCCCGTCAG 
GATGATGACT GGATTGTGGA TGACGATGGG ATAGGCTATG TGGAAGATGG CAGAGAGATT 
TTTGATGATG ACCTTGAGGA TGATGCACTT GACACCTGTG GAAAAGGAAG TGATGGCAAA 
GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC 
AAGGCAATGT TCATTGCTAG TGCCGGGAAG AAAACTACAG ATAAAGCAGT AGACTTGTCC 
AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTAAACA CTGAGACAGC TCAAATAACT 
CCACCACCTG TATTGATACC AAAGAAAAAA AGATCTACTG GGGCTTTGCT CAACCCTTTT 
TCAGTGCACA CCCCTAAGGC AATTCCTTCA GGAAAACCTG CTTCCCCTGT CCTCAGGAAT 
GAGCCTCTGT TAACTCCCAT TCCTCTTAAA CGTGCTGAAC TTGCTGGAGA GCTGGCTCAG 
CCAGAATGTC CAGAAGATGA GCAGGAGCTA GGGGTAATGG AGTTTGAAGA CGGTGACTTT 
GATGAGTCCA TGGACACAGA GAAGGTAGAT GAGAAGCCTG TGACTGCTAA GACTTGGGAC 
CAAGAGACTG AGCCAGTAGA AAGGGTGGAA CATGAGGCAG ATCCTGAGAG GGGAACCACA 
TCTTACTTAG AAAATTTTCT GCCAGATGTC TCTTGTTGGG ACATTGATCA GGATGATGAA 
AGCATACCTC AGGAAGTTCA AGTGGATTCC AGTAATCTCC CATTGGTAAA AGGGGCAGAT 
GATGAACAAG TGTTTCAGTT TTATTGGCTG GATGCTTATG AAGATCCATA CAACCAACCA 
GGTGTGGTTT TTCTGTTTGG CAAAGTGTGG ATTGAATCAG TCAAAACCCA TGTGAGCTGT 
TGTGTTATGG TGAAAAATAT TGAGCGAACA CTCTACTTCC TTCCCCGTGA AATGAAATTT 
GACCTAAACA CAGGGAAAGA AACAGCAATT CCAGTTACAA TGAAGGATGT TTATGAAGAA 
TTTGATTCAA AAATTTCAGC AAAATATAAA ATAATGAAGT TCAAGTCCAA GATAGTGGAA 
AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACCT GGAAGTTAGA 
TACTCGGCAG AAGTGCCACA GCTTCCACAG AATTTAAAAG GAGAAACATT TTCTCATGTG 
TTTGGAACTA ACACGTCTAG CTTGGAACTG TTCTTGATGA ATAGGAAGAT CAAAGGACCT 
TGTTGGCTTG AAGTAAAAAA TCCACAACTC TTGAATCAGC CAATCAGTTG GTGTAAATTT 
GAGGTAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCTCCTCCA 
CTTGTGGTGA TGTCTTTCAG CATGAAGACA ATGCAGAATG TACAGAACCA TCAACATGAG 
ATTATTGCTA TGGCGGCTTT GGTTCATCAC AGTTTTGCAT TAGATAAAGC ACCTCCAGAG 
CCTCCCTTTC AGACACATTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTCCCATGT 
GATTTCAAAG AAGTGATTTC GAAAAAGAAT ATGAAGGTTG AGATCGCTGC AACAGAAAGA 
ACCTTGATAG GATTTTTCCT TGCAAAAGTT CACAAAATTG ACCCTGACAT TCTTGTGGGT 
CATAATATTT GTAGTTTTGA ACTGGAAGTG CTGCTGCAAA GAATTAATGA GTGCAAGGTT 
CCTTACTGGT CCAAGATAGG TCGGCTGAGG AGATCCAACA TGCCAAAACT TGGGAGCCGG 
AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGCCGAATGA TATGTGATGT GGAAATTTCA 
GCAAAAGAAT TGATTCATTG TAAAAGCTAC CATTTGTCTG AACTTGTTCA GCAGATTCTG 
AAAACTGAGA GGATTGTAAT TCCAACAGAA AATATACGAA ACATGTATAG TGAATCTTCT 
TATCTGTTGT ACCTGTTGGA ACATATCTGG AAAGATGCCA GATTCATTTT GCAGATCATG 
TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG 
TCTAGGACAC TGATGGGTGG ACGATCTGAG CGAAATGAGT TTTTGTTGCT TCATGCATTT 
TATGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAACCTCA GCAAAAACTG 
GGAGATGAAG ATGAGGAAAT TGATGGGGAC ACCAATAAAT ACAAGAAAGG GCGTAAGAAA 
GCAACTTACG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA CAAGTTCATT 
TTGCTCCTGG ATTTCAACAG TTTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT 
ACAACAGTGC AAAGAGTTAC TTCAGAGGTG CAGAAAGCTA CAGAGGATGA GGAACAAGAA 
CAGATCCCTG AGTTGCCAGA TCCAAACTTA GAGATGGGCA TTTTGCCCAG AGAGATTCGG 
AAACTGGTAG AGCGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA TTTAAATCCA 
GACCTTGTTC TTCAGTATGA CATCCGACAG AAGGCTTTGA AGCTCACAGC AAACAGTATG 
TATGGTTGCC TGGGATTTTC GTACAGCAGA TTTTATGCTA AACCACTGGC TGCCTTGGTG 
ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGACA TGGTACAGAA GATGAATCTT 
GAAGTTATTT ATGGAGACAC AGATTCAATT ATGATAAATA CCAATAGCAC CAACCTGGAA 
GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAATTGTA CAAACTGCTT 
GAAATAGACA TTGATGCTGT TTTCAAGTCT CTGCTGTTGC TGAAGAAAAA GAAATATGCT 
GCTCTAGTTG TTGAACCAAC ATCAGATGGG AATTACATTA CCAAGCAGGA GCTGAAAGGA 
TTAGATATAG TTAGAAGAGA CTGGTGTGAT CTTGCCAAGG ACACTGGAAA CTTTGTCATT 
GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACCATTGTGG AAAACATTCA GAAGAGGTTA 
ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC 
AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAGAA AAAGCTTACC TCATGTACAT 
GTTGCACTGT GGATAAATTC TCAAGGAGGC AGGAAGGTGA AAGCTGGTGA TACGGTGTCC 
TATGTTATTT GTCAGGATGG GTCAAACCTC ACTGCAACTC AGAGGGCCTA TGCCCCAGAA 
CAATTGCAGA AACTTGACAA TTTAGCCATC GACACCCAAT ACTACTTGGC CCAGCAGATC 
CACCCTGTTG TGGCTCGGAT CTGTGAGCCA ATAGATGGAA TTGATGCTGT ACTCATTGCA 
TTATGGCTAG GACTTGACTC CACTCAATTT AGAGTTCATC AGTATCACAA AGATGAAGAG 
AATGATGCCC TCCTTGGTGG TCCAGCACAG CTAACTGATG AAGAGAAATA CAAGGATTGT 
GAAAAATTCA AATGCCTTTG TCCTTCATGT GGAACTGAAA ATATTTATGA TAATGTCTTT 
GAAGGCTCGG GACTGGATAT GGAACCCAGT TTGTATCGTT GCAGTAATGT TGATTGTAAG 
GTTTCACCGT TGACCTTCAT GGTACAGCTG AGCAACAAAT TGATCATGGA CATTAGACGG 
TGCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACTTG TTGCAGTCGC 
CTTCGACGTC TTCCCCTTCA TTTCTCCCGA AATGGGCCTC TGTGTCCAGT CTGCATGAAA 
GCTGTGCTTA GACCAGAGAT AAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pola1 ( XM_006527905.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527968.1 Mus musculus polymerase (DNA directed), alpha 1 (Pola1), transcript variant X1, mRNA.

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CloneID	OMu56208
Clone ID Related Accession (Same CDS sequence)	XM_006527905.4 , XM_006527905.3
Accession Version	XM_006527905.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	DNA polymerase alpha catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527905.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCA TGCACGAAGA GGACTGTAAA CTGGAGGCAA GCGCTGTGTC AGATTCAGGG 
AGTTTTGCAG CTTCCCGAGC TCGGCGAGAA AAGAAATCAA AGAAAGGACG TCAAGAAGCT 
TTAGAGAGAC TGAAAAAGGC TAAAGCTGGG GAGAAGTATA AATACGAAGT TGAGGACCTC 
ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCGA AACTGGTTCA GGCCCGTCAG 
GATGATGACT GGATTGTGGA TGACGATGGG ATAGGCTATG TGGAAGATGG CAGAGAGATT 
TTTGATGATG ACCTTGAGGA TGATGCACTT GACACCTGTG GAAAAGGAAG TGATGGCAAA 
GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC 
AAGGCAATGT TCATTGCTAG TGCCGGGAAG AAAACTACAG ATAAAGCAGT AGACTTGTCC 
AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTAAACA CTGAGACAGC TCAAATAACT 
CCACCACCTG TATTGATACC AAAGAAAAAA AGATCTACTG GGGCTTTGCT CAACCCTTTT 
TCAGTGCACA CCCCTAAGGC AATTCCTTCA GGAAAACCTG CTTCCCCTGT CCTCAGGAAT 
GAGCCTCTGT TAACTCCCAT TCCTCTTAAA CGTGCTGAAC TTGCTGGAGA GCTGGCTCAG 
CCAGAATGTC CAGAAGATGA GCAGGAGCTA GGGGTAATGG AGTTTGAAGA CGGTGACTTT 
GATGAGTCCA TGGACACAGA GAAGGTAGAT GAGAAGCCTG TGACTGCTAA GACTTGGGAC 
CAAGAGACTG AGCCAGTAGA AAGGGTGGAA CATGAGGCAG ATCCTGAGAG GGGAACCACA 
TCTTACTTAG AAAATTTTCT GCCAGATGTC TCTTGTTGGG ACATTGATCA GGATGATGAA 
AGCATACCTC AGGAAGTTCA AGTGGATTCC AGTAATCTCC CATTGGTAAA AGGGGCAGAT 
GATGAACAAG TGTTTCAGTT TTATTGGCTG GATGCTTATG AAGATCCATA CAACCAACCA 
GGTGTGGTTT TTCTGTTTGG CAAAGTGTGG ATTGAATCAG TCAAAACCCA TGTGAGCTGT 
TGTGTTATGG TGAAAAATAT TGAGCGAACA CTCTACTTCC TTCCCCGTGA AATGAAATTT 
GACCTAAACA CAGGGAAAGA AACAGCAATT CCAGTTACAA TGAAGGATGT TTATGAAGAA 
TTTGATTCAA AAATTTCAGC AAAATATAAA ATAATGAAGT TCAAGTCCAA GATAGTGGAA 
AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACCT GGAAGTTAGA 
TACTCGGCAG AAGTGCCACA GCTTCCACAG AATTTAAAAG GAGAAACATT TTCTCATGTG 
TTTGGAACTA ACACGTCTAG CTTGGAACTG TTCTTGATGA ATAGGAAGAT CAAAGGACCT 
TGTTGGCTTG AAGTAAAAAA TCCACAACTC TTGAATCAGC CAATCAGTTG GTGTAAATTT 
GAGGTAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCTCCTCCA 
CTTGTGGTGA TGTCTTTCAG CATGAAGACA ATGCAGAATG TACAGAACCA TCAACATGAG 
ATTATTGCTA TGGCGGCTTT GGTTCATCAC AGTTTTGCAT TAGATAAAGC ACCTCCAGAG 
CCTCCCTTTC AGACACATTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTCCCATGT 
GATTTCAAAG AAGTGATTTC GAAAAAGAAT ATGAAGGTTG AGATCGCTGC AACAGAAAGA 
ACCTTGATAG GATTTTTCCT TGCAAAAGTT CACAAAATTG ACCCTGACAT TCTTGTGGGT 
CATAATATTT GTAGTTTTGA ACTGGAAGTG CTGCTGCAAA GAATTAATGA GTGCAAGGTT 
CCTTACTGGT CCAAGATAGG TCGGCTGAGG AGATCCAACA TGCCAAAACT TGGGAGCCGG 
AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGCCGAATGA TATGTGATGT GGAAATTTCA 
GCAAAAGAAT TGATTCATTG TAAAAGCTAC CATTTGTCTG AACTTGTTCA GCAGATTCTG 
AAAACTGAGA GGATTGTAAT TCCAACAGAA AATATACGAA ACATGTATAG TGAATCTTCT 
TATCTGTTGT ACCTGTTGGA ACATATCTGG AAAGATGCCA GATTCATTTT GCAGATCATG 
TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG 
TCTAGGACAC TGATGGGTGG ACGATCTGAG CGAAATGAGT TTTTGTTGCT TCATGCATTT 
TATGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAACCTCA GCAAAAACTG 
GGAGATGAAG ATGAGGAAAT TGATGGGGAC ACCAATAAAT ACAAGAAAGG GCGTAAGAAA 
GCAACTTACG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA CAAGTTCATT 
TTGCTCCTGG ATTTCAACAG TTTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT 
ACAACAGTGC AAAGAGTTAC TTCAGAGGTG CAGAAAGCTA CAGAGGATGA GGAACAAGAA 
CAGATCCCTG AGTTGCCAGA TCCAAACTTA GAGATGGGCA TTTTGCCCAG AGAGATTCGG 
AAACTGGTAG AGCGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA TTTAAATCCA 
GACCTTGTTC TTCAGTATGA CATCCGACAG AAGGCTTTGA AGCTCACAGC AAACAGTATG 
TATGGTTGCC TGGGATTTTC GTACAGCAGA TTTTATGCTA AACCACTGGC TGCCTTGGTG 
ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGACA TGGTACAGAA GATGAATCTT 
GAAGTTATTT ATGGAGACAC AGATTCAATT ATGATAAATA CCAATAGCAC CAACCTGGAA 
GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAATTGTA CAAACTGCTT 
GAAATAGACA TTGATGCTGT TTTCAAGTCT CTGCTGTTGC TGAAGAAAAA GAAATATGCT 
GCTCTAGTTG TTGAACCAAC ATCAGATGGG AATTACATTA CCAAGCAGGA GCTGAAAGGA 
TTAGATATAG TTAGAAGAGA CTGGTGTGAT CTTGCCAAGG ACACTGGAAA CTTTGTCATT 
GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACCATTGTGG AAAACATTCA GAAGAGGTTA 
ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC 
AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAGAA AAAGCTTACC TCATGTACAT 
GTTGCACTGT GGATAAATTC TCAAGGAGGC AGGAAGGTGA AAGCTGGTGA TACGGTGTCC 
TATGTTATTT GTCAGGATGG GTCAAACCTC ACTGCAACTC AGAGGGCCTA TGCCCCAGAA 
CAATTGCAGA AACTTGACAA TTTAGCCATC GACACCCAAT ACTACTTGGC CCAGCAGATC 
CACCCTGTTG TGGCTCGGAT CTGTGAGCCA ATAGATGGAA TTGATGCTGT ACTCATTGCA 
TTATGGCTAG GACTTGACTC CACTCAATTT AGAGTTCATC AGTATCACAA AGATGAAGAG 
AATGATGCCC TCCTTGGTGG TCCAGCACAG CTAACTGATG AAGAGAAATA CAAGGATTGT 
GAAAAATTCA AATGCCTTTG TCCTTCATGT GGAACTGAAA ATATTTATGA TAATGTCTTT 
GAAGGCTCGG GACTGGATAT GGAACCCAGT TTGTATCGTT GCAGTAATGT TGATTGTAAG 
GTTTCACCGT TGACCTTCAT GGTACAGCTG AGCAACAAAT TGATCATGGA CATTAGACGG 
TGCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACTTG TTGCAGTCGC 
CTTCGACGTC TTCCCCTTCA TTTCTCCCGA AATGGGCCTC TGTGTCCAGT CTGCATGAAA 
GCTGTGCTTA GACCAGAGAT AAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527968.1
CDS	99..4265
Translation	MAPMHEEDCKLEASAVSDSGSFAASRARREKKSKKGRQEALERLKKAKAGEKYKYEVEDLTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDTCGKGSDGKAHRKDRKDVKKPSVTKPNNIKAMFIASAGKKTTDKAVDLSKDDLLGDILQDLNTETAQITPPPVLIPKKKRSTGALLNPFSVHTPKAIPSGKPASPVLRNEPLLTPIPLKRAELAGELAQPECPEDEQELGVMEFEDGDFDESMDTEKVDEKPVTAKTWDQETEPVERVEHEADPERGTTSYLENFLPDVSCWDIDQDDESIPQEVQVDSSNLPLVKGADDEQVFQFYWLDAYEDPYNQPGVVFLFGKVWIESVKTHVSCCVMVKNIERTLYFLPREMKFDLNTGKETAIPVTMKDVYEEFDSKISAKYKIMKFKSKIVEKNYAFEIPDVPEKSEYLEVRYSAEVPQLPQNLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKFEVMALKPDLVNVIKDVSPPPLVVMSFSMKTMQNVQNHQHEIIAMAALVHHSFALDKAPPEPPFQTHFCVVSKPKDCIFPCDFKEVISKKNMKVEIAATERTLIGFFLAKVHKIDPDILVGHNICSFELEVLLQRINECKVPYWSKIGRLRRSNMPKLGSRSGFGERNATCGRMICDVEISAKELIHCKSYHLSELVQQILKTERIVIPTENIRNMYSESSYLLYLLEHIWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKKATYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVTSEVQKATEDEEQEQIPELPDPNLEMGILPREIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKDMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDAVFKSLLLLKKKKYAALVVEPTSDGNYITKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDRKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTATQRAYAPEQLQKLDNLAIDTQYYLAQQIHPVVARICEPIDGIDAVLIALWLGLDSTQFRVHQYHKDEENDALLGGPAQLTDEEKYKDCEKFKCLCPSCGTENIYDNVFEGSGLDMEPSLYRCSNVDCKVSPLTFMVQLSNKLIMDIRRCIKKYYDGWLICEEPTCCSRLRRLPLHFSRNGPLCPVCMKAVLRPEIN

Target ORF information:

RefSeq Version	XM_006527905.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), alpha 1 (Pola1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527905.3

ORF Insert Sequence:

ATGGCGCCCA TGCACGAAGA GGACTGTAAA CTGGAGGCAA GCGCTGTGTC AGATTCAGGG 
AGTTTTGCAG CTTCCCGAGC TCGGCGAGAA AAGAAATCAA AGAAAGGACG TCAAGAAGCT 
TTAGAGAGAC TGAAAAAGGC TAAAGCTGGG GAGAAGTATA AATACGAAGT TGAGGACCTC 
ACAAGTGTTT ATGAAGAAGT TGATGAAGAG CAGTATTCGA AACTGGTTCA GGCCCGTCAG 
GATGATGACT GGATTGTGGA TGACGATGGG ATAGGCTATG TGGAAGATGG CAGAGAGATT 
TTTGATGATG ACCTTGAGGA TGATGCACTT GACACCTGTG GAAAAGGAAG TGATGGCAAA 
GCACACAGAA AGGACAGGAA GGATGTAAAG AAACCTTCAG TGACAAAACC AAACAATATC 
AAGGCAATGT TCATTGCTAG TGCCGGGAAG AAAACTACAG ATAAAGCAGT AGACTTGTCC 
AAGGATGATC TGCTAGGTGA CATTCTACAG GATCTAAACA CTGAGACAGC TCAAATAACT 
CCACCACCTG TATTGATACC AAAGAAAAAA AGATCTACTG GGGCTTTGCT CAACCCTTTT 
TCAGTGCACA CCCCTAAGGC AATTCCTTCA GGAAAACCTG CTTCCCCTGT CCTCAGGAAT 
GAGCCTCTGT TAACTCCCAT TCCTCTTAAA CGTGCTGAAC TTGCTGGAGA GCTGGCTCAG 
CCAGAATGTC CAGAAGATGA GCAGGAGCTA GGGGTAATGG AGTTTGAAGA CGGTGACTTT 
GATGAGTCCA TGGACACAGA GAAGGTAGAT GAGAAGCCTG TGACTGCTAA GACTTGGGAC 
CAAGAGACTG AGCCAGTAGA AAGGGTGGAA CATGAGGCAG ATCCTGAGAG GGGAACCACA 
TCTTACTTAG AAAATTTTCT GCCAGATGTC TCTTGTTGGG ACATTGATCA GGATGATGAA 
AGCATACCTC AGGAAGTTCA AGTGGATTCC AGTAATCTCC CATTGGTAAA AGGGGCAGAT 
GATGAACAAG TGTTTCAGTT TTATTGGCTG GATGCTTATG AAGATCCATA CAACCAACCA 
GGTGTGGTTT TTCTGTTTGG CAAAGTGTGG ATTGAATCAG TCAAAACCCA TGTGAGCTGT 
TGTGTTATGG TGAAAAATAT TGAGCGAACA CTCTACTTCC TTCCCCGTGA AATGAAATTT 
GACCTAAACA CAGGGAAAGA AACAGCAATT CCAGTTACAA TGAAGGATGT TTATGAAGAA 
TTTGATTCAA AAATTTCAGC AAAATATAAA ATAATGAAGT TCAAGTCCAA GATAGTGGAA 
AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACCT GGAAGTTAGA 
TACTCGGCAG AAGTGCCACA GCTTCCACAG AATTTAAAAG GAGAAACATT TTCTCATGTG 
TTTGGAACTA ACACGTCTAG CTTGGAACTG TTCTTGATGA ATAGGAAGAT CAAAGGACCT 
TGTTGGCTTG AAGTAAAAAA TCCACAACTC TTGAATCAGC CAATCAGTTG GTGTAAATTT 
GAGGTAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCTCCTCCA 
CTTGTGGTGA TGTCTTTCAG CATGAAGACA ATGCAGAATG TACAGAACCA TCAACATGAG 
ATTATTGCTA TGGCGGCTTT GGTTCATCAC AGTTTTGCAT TAGATAAAGC ACCTCCAGAG 
CCTCCCTTTC AGACACATTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTCCCATGT 
GATTTCAAAG AAGTGATTTC GAAAAAGAAT ATGAAGGTTG AGATCGCTGC AACAGAAAGA 
ACCTTGATAG GATTTTTCCT TGCAAAAGTT CACAAAATTG ACCCTGACAT TCTTGTGGGT 
CATAATATTT GTAGTTTTGA ACTGGAAGTG CTGCTGCAAA GAATTAATGA GTGCAAGGTT 
CCTTACTGGT CCAAGATAGG TCGGCTGAGG AGATCCAACA TGCCAAAACT TGGGAGCCGG 
AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGCCGAATGA TATGTGATGT GGAAATTTCA 
GCAAAAGAAT TGATTCATTG TAAAAGCTAC CATTTGTCTG AACTTGTTCA GCAGATTCTG 
AAAACTGAGA GGATTGTAAT TCCAACAGAA AATATACGAA ACATGTATAG TGAATCTTCT 
TATCTGTTGT ACCTGTTGGA ACATATCTGG AAAGATGCCA GATTCATTTT GCAGATCATG 
TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG 
TCTAGGACAC TGATGGGTGG ACGATCTGAG CGAAATGAGT TTTTGTTGCT TCATGCATTT 
TATGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAACCTCA GCAAAAACTG 
GGAGATGAAG ATGAGGAAAT TGATGGGGAC ACCAATAAAT ACAAGAAAGG GCGTAAGAAA 
GCAACTTACG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA CAAGTTCATT 
TTGCTCCTGG ATTTCAACAG TTTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT 
ACAACAGTGC AAAGAGTTAC TTCAGAGGTG CAGAAAGCTA CAGAGGATGA GGAACAAGAA 
CAGATCCCTG AGTTGCCAGA TCCAAACTTA GAGATGGGCA TTTTGCCCAG AGAGATTCGG 
AAACTGGTAG AGCGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA TTTAAATCCA 
GACCTTGTTC TTCAGTATGA CATCCGACAG AAGGCTTTGA AGCTCACAGC AAACAGTATG 
TATGGTTGCC TGGGATTTTC GTACAGCAGA TTTTATGCTA AACCACTGGC TGCCTTGGTG 
ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGACA TGGTACAGAA GATGAATCTT 
GAAGTTATTT ATGGAGACAC AGATTCAATT ATGATAAATA CCAATAGCAC CAACCTGGAA 
GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAATTGTA CAAACTGCTT 
GAAATAGACA TTGATGCTGT TTTCAAGTCT CTGCTGTTGC TGAAGAAAAA GAAATATGCT 
GCTCTAGTTG TTGAACCAAC ATCAGATGGG AATTACATTA CCAAGCAGGA GCTGAAAGGA 
TTAGATATAG TTAGAAGAGA CTGGTGTGAT CTTGCCAAGG ACACTGGAAA CTTTGTCATT 
GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACCATTGTGG AAAACATTCA GAAGAGGTTA 
ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC 
AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAGAA AAAGCTTACC TCATGTACAT 
GTTGCACTGT GGATAAATTC TCAAGGAGGC AGGAAGGTGA AAGCTGGTGA TACGGTGTCC 
TATGTTATTT GTCAGGATGG GTCAAACCTC ACTGCAACTC AGAGGGCCTA TGCCCCAGAA 
CAATTGCAGA AACTTGACAA TTTAGCCATC GACACCCAAT ACTACTTGGC CCAGCAGATC 
CACCCTGTTG TGGCTCGGAT CTGTGAGCCA ATAGATGGAA TTGATGCTGT ACTCATTGCA 
TTATGGCTAG GACTTGACTC CACTCAATTT AGAGTTCATC AGTATCACAA AGATGAAGAG 
AATGATGCCC TCCTTGGTGG TCCAGCACAG CTAACTGATG AAGAGAAATA CAAGGATTGT 
GAAAAATTCA AATGCCTTTG TCCTTCATGT GGAACTGAAA ATATTTATGA TAATGTCTTT 
GAAGGCTCGG GACTGGATAT GGAACCCAGT TTGTATCGTT GCAGTAATGT TGATTGTAAG 
GTTTCACCGT TGACCTTCAT GGTACAGCTG AGCAACAAAT TGATCATGGA CATTAGACGG 
TGCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACTTG TTGCAGTCGC 
CTTCGACGTC TTCCCCTTCA TTTCTCCCGA AATGGGCCTC TGTGTCCAGT CTGCATGAAA 
GCTGTGCTTA GACCAGAGAT AAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis.
Molecular cell70(4)707-721.e7(2018 05)
Bellelli R,Borel V,Logan C,Svendsen J,Cox DE,Nye E,Metcalfe K,O'Connell SM,Stamp G,Flynn HR,Snijders AP,Lassailly F,Jackson A,Boulton SJ

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Immunity43(4)690-702(2015 Oct)
Ray JP,Staron MM,Shyer JA,Ho PC,Marshall HD,Gray SM,Laidlaw BJ,Araki K,Ahmed R,Kaech SM,Craft J

Phenotypic annotation of the mouse X chromosome.
Genome research20(8)1154-64(2010 Aug)
Cox BJ,Vollmer M,Tamplin O,Lu M,Biechele S,Gertsenstein M,van Campenhout C,Floss T,Kühn R,Wurst W,Lickert H,Rossant J

Aberrant DNA polymerase alpha is excluded from the nucleus by defective import and degradation in the nucleus.
The Journal of biological chemistry284(44)30604-14(2009 Oct)
Eichinger CS,Mizuno T,Mizuno K,Miyake Y,Yanagi K,Imamoto N,Hanaoka F

Anti-tumor effects of dehydroaltenusin, a specific inhibitor of mammalian DNA polymerase alpha.
Biochemical and biophysical research communications352(2)390-6(2007 Jan)
Maeda N,Kokai Y,Ohtani S,Sahara H,Kuriyama I,Kamisuki S,Takahashi S,Sakaguchi K,Sugawara F,Yoshida H,Sato N,Mizushina Y

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Pola1 cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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