POLA1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLA1
Entrez Gene ID	480869
Full Name	DNA polymerase alpha 1, catalytic subunit
General protein information	Preferred Names DNA polymerase alpha 1, catalytic subunit Names DNA polymerase alpha catalytic subunit polymerase (DNA directed), alpha 1, catalytic subunit
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005641214.3	XP_005641271.1	DNA polymerase alpha catalytic subunit
XM_005641214.2	XP_005641271.1	DNA polymerase alpha catalytic subunit

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AER381C	NP_985237.1
Macaca mulatta (Rhesus monkey)	POLA1	XP_001091195.1
Gallus gallus (chicken)	POLA1	XP_416792.3
Homo sapiens (human)	POLA1	NP_058633.2
Rattus norvegicus (Norway rat)	Pola1	NP_445931.1
Schizosaccharomyces pombe (fission yeast)	pol1	NP_594186.1
Neurospora crassa	NCU07870	XP_963131.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001929	XP_321134.5
Magnaporthe oryzae (rice blast fungus)	MGG_06397	XP_003717174.1
Mus musculus (house mouse)	Pola1	NP_032918.1
Danio rerio (zebrafish)	pola1	XP_005162809.1
Drosophila melanogaster (fruit fly)	DNApol-alpha180	NP_536736.2
Caenorhabditis elegans (roundworm)	Y47D3A.29	NP_001255109.1
Arabidopsis thaliana (thale cress)	ICU2	NP_201511.2
Xenopus tropicalis (tropical clawed frog)	pola1	XP_004911812.1
Pan troglodytes (chimpanzee)	POLA1	XP_003317445.2
Kluyveromyces lactis	KLLA0C13255g	XP_452791.1
Bos taurus (cattle)	POLA1	NP_001192994.1
Saccharomyces cerevisiae (baker's yeast)	POL1	NP_014297.3
Canis lupus familiaris (dog)	POLA1	XP_005641271.1
Oryza sativa (rice)	Os01g0868300	NP_001044920.1

Gene Ontology

The following POLA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe32205	XM_005641214.3 Latest version!	Canis lupus familiaris DNA polymerase alpha 1, catalytic subunit (POLA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OCe32205	XM_005641214.2	Canis lupus familiaris polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLA1 ( XM_005641214.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005641271.1 Canis lupus familiaris DNA polymerase alpha 1, catalytic subunit (POLA1), mRNA.

Price & Availability↑

CloneID	OCe32205
Clone ID Related Accession (Same CDS sequence)	XM_005641214.3 , XM_005641214.2
Accession Version	XM_005641214.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	DNA polymerase alpha catalytic subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641214.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGG TACACGGCGA AGACTGCGAC CTGGGGTCGA GCGCTGTGTC AGATTCAGGG 
AGTTTTGTAT CTTCTCGATC CCGGCGAGAA AAAAAATCAA AGAGAGGGCG CCAAGAAGCT 
CTAGAAAGAC TGAAAAGGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT TGAGGACTTC 
ACAAGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCCCGCCAG 
GATGATGACT GGATCGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT 
TTTGATGATG ACCTTGAAGA CGATGCCCTT GATTCTCATG AGAAAGGAAA AGATGATAAA 
GCACGTACCA AAGACAGGAG GAATGTAAAG AAGGCTGCAG TGACAAAACC AAACAATATC 
AAGTCAATGT TCATTGCTAG TGCTGGGAAG AAGACTACAG ATAAAGCTGT AGACTTGTCC 
AAGGATGATC TGCTAGGTGA CATTCTACAA GATCTGAACA CTGAGAAACC TCCAGTAACT 
CCACCACCTA TAATGATACC AAAGAAGAAA AGATCCATTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGTTCCTTCA GGAAAGACTG CTTCCCCTGT CTCAAGGAAG 
GAGCCTCCAT TAACTCCAGT TCCTGTAAAA TGTGCTGAAT TTGCTGGAGA GCCGGTGCCA 
GCGTTGTGTA TAGATGACAA AGAGGACTCA GGAGCGATGG ATTTTGAAGA TGGTGACTTT 
GATGAGCCCA TGGAGGCTGA GGAAGTGGAT GTGGAGCCTG TGGCTGTCAA GACTTGGCAC 
CAAGAGAGTG AGCCAGCAGA GGGGGTGAAG TATGAGGCAG ATCCTGGGAA AGGGACCACG 
TCCTACTTAG GAAGTTTTCT CCCAGATGTG TCTTGTTGGG ACATTGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCAGGA AGTCCAAGTG GATTCCAGTC ACCTCCCCCT GGTGAAAGGG 
ACAGATGAGG AGCAAGTGTT TCATTTTTAT TGGCTGGATG CTTATGAAGA TCAGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAA GTTTGGATTG AATCTGCCAA AACCCATGTG 
AGCTGTTGTG TTATGGTGAA AAACATCGAG CGAACACTCT ACTTCCTTCC CCGAGAAATG 
AAAATTGATC TAAATACAGG AAAAGAAACA GAAACTCTAG TTACAATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAAATCA TGAAGTTCAA GTCTAAGCCA 
GTGGAAAAGA ATTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTGTTTG GGACCAACAC ATCCAGCCTA GAAATGTTCT TGATGAACAG AAAGATCAAG 
GGACCTTGTT GGCTCGAGGT GAAAAATCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT 
AAAGTTGAGG CAATGGCCTT GAAACCAGAC CTGGTGAATG TAATTAAAGA TATTGGTCCT 
CCTCCACTCG TGGTCATGTC TTTCAGCATG AAGACAATGC AGAATGCAAA GACACATCAA 
CATGAGATTA TTGCTGTGGC AGCTTTGGTC CATCACAGCT TTGCATTAGA TAAAGCGGCC 
CCACAGCCTC CCTTCCAGTC ACACTTCTGT GTTGTATCTA AACCAAAGGA CTGTATTTTC 
CCATATACTT TCAAAGACTA CATTAAGGAA AAGAATGTGA AGGTTGAGAT TGCTGCAACA 
GAAAGAACAT TACTAGGTTT TTTCCTCGCC AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG ATTTGAACTG GAAGTGCTCC TGCAGAGAAT TAATGTATGC 
AAAGTTCCTC ACTGGTCCAA AATAGGTCGA CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATT TGTCTGAACT TGTTCAACAG 
ATTTTGAGAA CTGAGAGGGT TGTAATCCCA ATGGAAAATG TACGAAATAT GTACAGTGAA 
TCTTCTCATC TGCTGTACCT GTTGGAACAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACGCTGAT GGGTGGACGA TCTGAGCGTA ATGAGTTCTT GTTACTTCAT 
GCGTTTTATG AAAACAACTA TATTGTGCCC GACAAGCAGA TTTTCAGAAA GCCTCAGCAA 
AAACTGGGAG ATGAAGATGA AGAACTTGAT GGAGATGCCA ATAAATACAA GAAAGGACGT 
AAGAAAGCCG CTTATGCTGG AGGCTTGGTA TTGGACCCTA AAGCTGGTTT TTATGATAAG 
TTTATTTTGC TCCTGGACTT CAACAGTCTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGACACAGA AAGTTACGGA GGATGGAGAA 
CAAGAACAGA TCCCTGAGCT ACCAGACCCA GGCTTAGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGGAAAC TGGTAGAGAG GAGAAAACAA GTCAAACAAC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTGTTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT AACAGCAAAC 
AGTATGTATG GCTGCCTGGG ATTTTCCTAC AGCAGATTTT ATGCCAAACC ACTGGCCGCT 
CTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACAA AAGAGATGGT ACAAAAGATG 
AACCTTGAAG TTATTTATGG AGATACTGAT TCAATTATGA TAAACACCAA TAGCACCGAT 
TTGGAAGAAG TATATAAATT GGGAAACAAG GTAAAAAGTG AAGTCAACAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA CGGTGTTTTC AAGTCTCTGC TACTGCTGAA GAAAAAGAAG 
TATGGTGCTC TGGTTGTTGA GCCAACGTCA GATGGGAATT ACTCCACCAA ACAGGAACTG 
AAAGGGTTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGAAAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC TGATCAAAGC CGAGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG TCTACCTCAT 
GTACATGTTG CCCTCTGGAT GAATTCTCAA GGAGGCAGAA AAATGAAAGC TGGAGATACT 
GTGTCATATG TCATCTGTCA GGATGGGTCA AACCTCACTG CGAGTCAGAG GGCCTATGCA 
CCTGAGCAGC TACAGAAACA GGACAATTTA ACCATTGACA CCCAGTACTA CCTGGCTCAG 
CAGATCCACC CAGTCGTGGC TCGGATCTGT GAGCCAATAG ATGGAATTGA TGCTGTCCTG 
ATTGCAACAT GGTTAGGACT CGACCCCACC CAATTTAGAG TTCATCATTA TCATAAGGAT 
GAGGAGAATG ATGCCCTACT TGGTGGCCCA GCACAGCTCA CTGATGAGGA GAAATACAGG 
GACTGTGAAA GATTCAAATG CCCATGCCCC ACATGTGGAA CAGAAAATAT ATACGATAGT 
GTCTTGGATG GTTCGGGAAC TGATGTGGAG CCCAGCTTGT ATCGCTGCAG CAACATTGAT 
TGTAAGGCTT CACCTTTGAC CTCTGTGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGT TGGTTGATTT GTGAGGAGCC AACGTGTCAA 
AATCGAACTC GGCGCCTTCC CCTTCAGTTC TCCCGAAATG GGCCTCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGGCC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT 
TACCGGTACA TTTTTGATGT GGACTGTGCA CTGGAGAAAC TTAGTACTGA TCAGGAGAAA 
GATAAGTTGA AGAAGGTTTT TACCTTAAAA GTTCGTCAGG ACTACCAGAA ACTCAGGAAC 
ACAGCAGAGC AGTTCCTGTC CCGAAGTGGC TACTCGGAAG TGAACCTCAG CAAACTCTTC 
GCAGACTGTG CTGTGAGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641271.1
CDS	115..4518
Translation	MALVHGEDCDLGSSAVSDSGSFVSSRSRREKKSKRGRQEALERLKRAKAGEKYKYEVEDFTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDSHEKGKDDKARTKDRRNVKKAAVTKPNNIKSMFIASAGKKTTDKAVDLSKDDLLGDILQDLNTEKPPVTPPPIMIPKKKRSIGASLNPFSVHTPKAVPSGKTASPVSRKEPPLTPVPVKCAEFAGEPVPALCIDDKEDSGAMDFEDGDFDEPMEAEEVDVEPVAVKTWHQESEPAEGVKYEADPGKGTTSYLGSFLPDVSCWDIDQEDDSSFSAQEVQVDSSHLPLVKGTDEEQVFHFYWLDAYEDQYSQPGVVFLFGKVWIESAKTHVSCCVMVKNIERTLYFLPREMKIDLNTGKETETLVTMKDVYDEFDEKIAAKYKIMKFKSKPVEKNYAFEIPDVPEKSEYLEVRYSAEMPQLPQDLKGETFSHVFGTNTSSLEMFLMNRKIKGPCWLEVKNPQLLNQPVSWCKVEAMALKPDLVNVIKDIGPPPLVVMSFSMKTMQNAKTHQHEIIAVAALVHHSFALDKAAPQPPFQSHFCVVSKPKDCIFPYTFKDYIKEKNVKVEIAATERTLLGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKVPHWSKIGRLKRSNMPKLGGRSGFGERNATCGRMICDVEISAKELIRCKSYHLSELVQQILRTERVVIPMENVRNMYSESSHLLYLLEHTWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKLGDEDEELDGDANKYKKGRKKAAYAGGLVLDPKAGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASETQKVTEDGEQEQIPELPDPGLEMGILPREIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTDLEEVYKLGNKVKSEVNKLYKLLEIDIDGVFKSLLLLKKKKYGALVVEPTSDGNYSTKQELKGLDIVRRDWCDLAKETGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWMNSQGGRKMKAGDTVSYVICQDGSNLTASQRAYAPEQLQKQDNLTIDTQYYLAQQIHPVVARICEPIDGIDAVLIATWLGLDPTQFRVHHYHKDEENDALLGGPAQLTDEEKYRDCERFKCPCPTCGTENIYDSVLDGSGTDVEPSLYRCSNIDCKASPLTSVVQLSNKLIMDIRRCIKKYYEGWLICEEPTCQNRTRRLPLQFSRNGPLCQVCMKATLRPEYSDKSLYTQLCFYRYIFDVDCALEKLSTDQEKDKLKKVFTLKVRQDYQKLRNTAEQFLSRSGYSEVNLSKLFADCAVRP

Target ORF information:

RefSeq Version	XM_005641214.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DNA polymerase alpha 1, catalytic subunit (POLA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641214.3

ORF Insert Sequence:

ATGGCGCTGG TACACGGCGA AGACTGCGAC CTGGGGTCGA GCGCTGTGTC AGATTCAGGG 
AGTTTTGTAT CTTCTCGATC CCGGCGAGAA AAAAAATCAA AGAGAGGGCG CCAAGAAGCT 
CTAGAAAGAC TGAAAAGGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT TGAGGACTTC 
ACAAGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCCCGCCAG 
GATGATGACT GGATCGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT 
TTTGATGATG ACCTTGAAGA CGATGCCCTT GATTCTCATG AGAAAGGAAA AGATGATAAA 
GCACGTACCA AAGACAGGAG GAATGTAAAG AAGGCTGCAG TGACAAAACC AAACAATATC 
AAGTCAATGT TCATTGCTAG TGCTGGGAAG AAGACTACAG ATAAAGCTGT AGACTTGTCC 
AAGGATGATC TGCTAGGTGA CATTCTACAA GATCTGAACA CTGAGAAACC TCCAGTAACT 
CCACCACCTA TAATGATACC AAAGAAGAAA AGATCCATTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGTTCCTTCA GGAAAGACTG CTTCCCCTGT CTCAAGGAAG 
GAGCCTCCAT TAACTCCAGT TCCTGTAAAA TGTGCTGAAT TTGCTGGAGA GCCGGTGCCA 
GCGTTGTGTA TAGATGACAA AGAGGACTCA GGAGCGATGG ATTTTGAAGA TGGTGACTTT 
GATGAGCCCA TGGAGGCTGA GGAAGTGGAT GTGGAGCCTG TGGCTGTCAA GACTTGGCAC 
CAAGAGAGTG AGCCAGCAGA GGGGGTGAAG TATGAGGCAG ATCCTGGGAA AGGGACCACG 
TCCTACTTAG GAAGTTTTCT CCCAGATGTG TCTTGTTGGG ACATTGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCAGGA AGTCCAAGTG GATTCCAGTC ACCTCCCCCT GGTGAAAGGG 
ACAGATGAGG AGCAAGTGTT TCATTTTTAT TGGCTGGATG CTTATGAAGA TCAGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAA GTTTGGATTG AATCTGCCAA AACCCATGTG 
AGCTGTTGTG TTATGGTGAA AAACATCGAG CGAACACTCT ACTTCCTTCC CCGAGAAATG 
AAAATTGATC TAAATACAGG AAAAGAAACA GAAACTCTAG TTACAATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAAATCA TGAAGTTCAA GTCTAAGCCA 
GTGGAAAAGA ATTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTGTTTG GGACCAACAC ATCCAGCCTA GAAATGTTCT TGATGAACAG AAAGATCAAG 
GGACCTTGTT GGCTCGAGGT GAAAAATCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT 
AAAGTTGAGG CAATGGCCTT GAAACCAGAC CTGGTGAATG TAATTAAAGA TATTGGTCCT 
CCTCCACTCG TGGTCATGTC TTTCAGCATG AAGACAATGC AGAATGCAAA GACACATCAA 
CATGAGATTA TTGCTGTGGC AGCTTTGGTC CATCACAGCT TTGCATTAGA TAAAGCGGCC 
CCACAGCCTC CCTTCCAGTC ACACTTCTGT GTTGTATCTA AACCAAAGGA CTGTATTTTC 
CCATATACTT TCAAAGACTA CATTAAGGAA AAGAATGTGA AGGTTGAGAT TGCTGCAACA 
GAAAGAACAT TACTAGGTTT TTTCCTCGCC AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG ATTTGAACTG GAAGTGCTCC TGCAGAGAAT TAATGTATGC 
AAAGTTCCTC ACTGGTCCAA AATAGGTCGA CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATT TGTCTGAACT TGTTCAACAG 
ATTTTGAGAA CTGAGAGGGT TGTAATCCCA ATGGAAAATG TACGAAATAT GTACAGTGAA 
TCTTCTCATC TGCTGTACCT GTTGGAACAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACGCTGAT GGGTGGACGA TCTGAGCGTA ATGAGTTCTT GTTACTTCAT 
GCGTTTTATG AAAACAACTA TATTGTGCCC GACAAGCAGA TTTTCAGAAA GCCTCAGCAA 
AAACTGGGAG ATGAAGATGA AGAACTTGAT GGAGATGCCA ATAAATACAA GAAAGGACGT 
AAGAAAGCCG CTTATGCTGG AGGCTTGGTA TTGGACCCTA AAGCTGGTTT TTATGATAAG 
TTTATTTTGC TCCTGGACTT CAACAGTCTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGACACAGA AAGTTACGGA GGATGGAGAA 
CAAGAACAGA TCCCTGAGCT ACCAGACCCA GGCTTAGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGGAAAC TGGTAGAGAG GAGAAAACAA GTCAAACAAC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTGTTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT AACAGCAAAC 
AGTATGTATG GCTGCCTGGG ATTTTCCTAC AGCAGATTTT ATGCCAAACC ACTGGCCGCT 
CTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACAA AAGAGATGGT ACAAAAGATG 
AACCTTGAAG TTATTTATGG AGATACTGAT TCAATTATGA TAAACACCAA TAGCACCGAT 
TTGGAAGAAG TATATAAATT GGGAAACAAG GTAAAAAGTG AAGTCAACAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA CGGTGTTTTC AAGTCTCTGC TACTGCTGAA GAAAAAGAAG 
TATGGTGCTC TGGTTGTTGA GCCAACGTCA GATGGGAATT ACTCCACCAA ACAGGAACTG 
AAAGGGTTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGAAAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC TGATCAAAGC CGAGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG TCTACCTCAT 
GTACATGTTG CCCTCTGGAT GAATTCTCAA GGAGGCAGAA AAATGAAAGC TGGAGATACT 
GTGTCATATG TCATCTGTCA GGATGGGTCA AACCTCACTG CGAGTCAGAG GGCCTATGCA 
CCTGAGCAGC TACAGAAACA GGACAATTTA ACCATTGACA CCCAGTACTA CCTGGCTCAG 
CAGATCCACC CAGTCGTGGC TCGGATCTGT GAGCCAATAG ATGGAATTGA TGCTGTCCTG 
ATTGCAACAT GGTTAGGACT CGACCCCACC CAATTTAGAG TTCATCATTA TCATAAGGAT 
GAGGAGAATG ATGCCCTACT TGGTGGCCCA GCACAGCTCA CTGATGAGGA GAAATACAGG 
GACTGTGAAA GATTCAAATG CCCATGCCCC ACATGTGGAA CAGAAAATAT ATACGATAGT 
GTCTTGGATG GTTCGGGAAC TGATGTGGAG CCCAGCTTGT ATCGCTGCAG CAACATTGAT 
TGTAAGGCTT CACCTTTGAC CTCTGTGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGT TGGTTGATTT GTGAGGAGCC AACGTGTCAA 
AATCGAACTC GGCGCCTTCC CCTTCAGTTC TCCCGAAATG GGCCTCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGGCC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT 
TACCGGTACA TTTTTGATGT GGACTGTGCA CTGGAGAAAC TTAGTACTGA TCAGGAGAAA 
GATAAGTTGA AGAAGGTTTT TACCTTAAAA GTTCGTCAGG ACTACCAGAA ACTCAGGAAC 
ACAGCAGAGC AGTTCCTGTC CCGAAGTGGC TACTCGGAAG TGAACCTCAG CAAACTCTTC 
GCAGACTGTG CTGTGAGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLA1 ( XM_005641214.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005641271.1 Canis lupus familiaris polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), mRNA.

Price & Availability↑

CloneID	OCe32205
Clone ID Related Accession (Same CDS sequence)	XM_005641214.3 , XM_005641214.2
Accession Version	XM_005641214.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	DNA polymerase alpha catalytic subunit
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641214.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGG TACACGGCGA AGACTGCGAC CTGGGGTCGA GCGCTGTGTC AGATTCAGGG 
AGTTTTGTAT CTTCTCGATC CCGGCGAGAA AAAAAATCAA AGAGAGGGCG CCAAGAAGCT 
CTAGAAAGAC TGAAAAGGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT TGAGGACTTC 
ACAAGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCCCGCCAG 
GATGATGACT GGATCGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT 
TTTGATGATG ACCTTGAAGA CGATGCCCTT GATTCTCATG AGAAAGGAAA AGATGATAAA 
GCACGTACCA AAGACAGGAG GAATGTAAAG AAGGCTGCAG TGACAAAACC AAACAATATC 
AAGTCAATGT TCATTGCTAG TGCTGGGAAG AAGACTACAG ATAAAGCTGT AGACTTGTCC 
AAGGATGATC TGCTAGGTGA CATTCTACAA GATCTGAACA CTGAGAAACC TCCAGTAACT 
CCACCACCTA TAATGATACC AAAGAAGAAA AGATCCATTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGTTCCTTCA GGAAAGACTG CTTCCCCTGT CTCAAGGAAG 
GAGCCTCCAT TAACTCCAGT TCCTGTAAAA TGTGCTGAAT TTGCTGGAGA GCCGGTGCCA 
GCGTTGTGTA TAGATGACAA AGAGGACTCA GGAGCGATGG ATTTTGAAGA TGGTGACTTT 
GATGAGCCCA TGGAGGCTGA GGAAGTGGAT GTGGAGCCTG TGGCTGTCAA GACTTGGCAC 
CAAGAGAGTG AGCCAGCAGA GGGGGTGAAG TATGAGGCAG ATCCTGGGAA AGGGACCACG 
TCCTACTTAG GAAGTTTTCT CCCAGATGTG TCTTGTTGGG ACATTGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCAGGA AGTCCAAGTG GATTCCAGTC ACCTCCCCCT GGTGAAAGGG 
ACAGATGAGG AGCAAGTGTT TCATTTTTAT TGGCTGGATG CTTATGAAGA TCAGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAA GTTTGGATTG AATCTGCCAA AACCCATGTG 
AGCTGTTGTG TTATGGTGAA AAACATCGAG CGAACACTCT ACTTCCTTCC CCGAGAAATG 
AAAATTGATC TAAATACAGG AAAAGAAACA GAAACTCTAG TTACAATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAAATCA TGAAGTTCAA GTCTAAGCCA 
GTGGAAAAGA ATTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTGTTTG GGACCAACAC ATCCAGCCTA GAAATGTTCT TGATGAACAG AAAGATCAAG 
GGACCTTGTT GGCTCGAGGT GAAAAATCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT 
AAAGTTGAGG CAATGGCCTT GAAACCAGAC CTGGTGAATG TAATTAAAGA TATTGGTCCT 
CCTCCACTCG TGGTCATGTC TTTCAGCATG AAGACAATGC AGAATGCAAA GACACATCAA 
CATGAGATTA TTGCTGTGGC AGCTTTGGTC CATCACAGCT TTGCATTAGA TAAAGCGGCC 
CCACAGCCTC CCTTCCAGTC ACACTTCTGT GTTGTATCTA AACCAAAGGA CTGTATTTTC 
CCATATACTT TCAAAGACTA CATTAAGGAA AAGAATGTGA AGGTTGAGAT TGCTGCAACA 
GAAAGAACAT TACTAGGTTT TTTCCTCGCC AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG ATTTGAACTG GAAGTGCTCC TGCAGAGAAT TAATGTATGC 
AAAGTTCCTC ACTGGTCCAA AATAGGTCGA CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATT TGTCTGAACT TGTTCAACAG 
ATTTTGAGAA CTGAGAGGGT TGTAATCCCA ATGGAAAATG TACGAAATAT GTACAGTGAA 
TCTTCTCATC TGCTGTACCT GTTGGAACAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACGCTGAT GGGTGGACGA TCTGAGCGTA ATGAGTTCTT GTTACTTCAT 
GCGTTTTATG AAAACAACTA TATTGTGCCC GACAAGCAGA TTTTCAGAAA GCCTCAGCAA 
AAACTGGGAG ATGAAGATGA AGAACTTGAT GGAGATGCCA ATAAATACAA GAAAGGACGT 
AAGAAAGCCG CTTATGCTGG AGGCTTGGTA TTGGACCCTA AAGCTGGTTT TTATGATAAG 
TTTATTTTGC TCCTGGACTT CAACAGTCTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGACACAGA AAGTTACGGA GGATGGAGAA 
CAAGAACAGA TCCCTGAGCT ACCAGACCCA GGCTTAGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGGAAAC TGGTAGAGAG GAGAAAACAA GTCAAACAAC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTGTTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT AACAGCAAAC 
AGTATGTATG GCTGCCTGGG ATTTTCCTAC AGCAGATTTT ATGCCAAACC ACTGGCCGCT 
CTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACAA AAGAGATGGT ACAAAAGATG 
AACCTTGAAG TTATTTATGG AGATACTGAT TCAATTATGA TAAACACCAA TAGCACCGAT 
TTGGAAGAAG TATATAAATT GGGAAACAAG GTAAAAAGTG AAGTCAACAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA CGGTGTTTTC AAGTCTCTGC TACTGCTGAA GAAAAAGAAG 
TATGGTGCTC TGGTTGTTGA GCCAACGTCA GATGGGAATT ACTCCACCAA ACAGGAACTG 
AAAGGGTTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGAAAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC TGATCAAAGC CGAGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG TCTACCTCAT 
GTACATGTTG CCCTCTGGAT GAATTCTCAA GGAGGCAGAA AAATGAAAGC TGGAGATACT 
GTGTCATATG TCATCTGTCA GGATGGGTCA AACCTCACTG CGAGTCAGAG GGCCTATGCA 
CCTGAGCAGC TACAGAAACA GGACAATTTA ACCATTGACA CCCAGTACTA CCTGGCTCAG 
CAGATCCACC CAGTCGTGGC TCGGATCTGT GAGCCAATAG ATGGAATTGA TGCTGTCCTG 
ATTGCAACAT GGTTAGGACT CGACCCCACC CAATTTAGAG TTCATCATTA TCATAAGGAT 
GAGGAGAATG ATGCCCTACT TGGTGGCCCA GCACAGCTCA CTGATGAGGA GAAATACAGG 
GACTGTGAAA GATTCAAATG CCCATGCCCC ACATGTGGAA CAGAAAATAT ATACGATAGT 
GTCTTGGATG GTTCGGGAAC TGATGTGGAG CCCAGCTTGT ATCGCTGCAG CAACATTGAT 
TGTAAGGCTT CACCTTTGAC CTCTGTGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGT TGGTTGATTT GTGAGGAGCC AACGTGTCAA 
AATCGAACTC GGCGCCTTCC CCTTCAGTTC TCCCGAAATG GGCCTCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGGCC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT 
TACCGGTACA TTTTTGATGT GGACTGTGCA CTGGAGAAAC TTAGTACTGA TCAGGAGAAA 
GATAAGTTGA AGAAGGTTTT TACCTTAAAA GTTCGTCAGG ACTACCAGAA ACTCAGGAAC 
ACAGCAGAGC AGTTCCTGTC CCGAAGTGGC TACTCGGAAG TGAACCTCAG CAAACTCTTC 
GCAGACTGTG CTGTGAGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641271.1
CDS	178..4581
Translation	MALVHGEDCDLGSSAVSDSGSFVSSRSRREKKSKRGRQEALERLKRAKAGEKYKYEVEDFTSVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDSHEKGKDDKARTKDRRNVKKAAVTKPNNIKSMFIASAGKKTTDKAVDLSKDDLLGDILQDLNTEKPPVTPPPIMIPKKKRSIGASLNPFSVHTPKAVPSGKTASPVSRKEPPLTPVPVKCAEFAGEPVPALCIDDKEDSGAMDFEDGDFDEPMEAEEVDVEPVAVKTWHQESEPAEGVKYEADPGKGTTSYLGSFLPDVSCWDIDQEDDSSFSAQEVQVDSSHLPLVKGTDEEQVFHFYWLDAYEDQYSQPGVVFLFGKVWIESAKTHVSCCVMVKNIERTLYFLPREMKIDLNTGKETETLVTMKDVYDEFDEKIAAKYKIMKFKSKPVEKNYAFEIPDVPEKSEYLEVRYSAEMPQLPQDLKGETFSHVFGTNTSSLEMFLMNRKIKGPCWLEVKNPQLLNQPVSWCKVEAMALKPDLVNVIKDIGPPPLVVMSFSMKTMQNAKTHQHEIIAVAALVHHSFALDKAAPQPPFQSHFCVVSKPKDCIFPYTFKDYIKEKNVKVEIAATERTLLGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKVPHWSKIGRLKRSNMPKLGGRSGFGERNATCGRMICDVEISAKELIRCKSYHLSELVQQILRTERVVIPMENVRNMYSESSHLLYLLEHTWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKLGDEDEELDGDANKYKKGRKKAAYAGGLVLDPKAGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASETQKVTEDGEQEQIPELPDPGLEMGILPREIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTDLEEVYKLGNKVKSEVNKLYKLLEIDIDGVFKSLLLLKKKKYGALVVEPTSDGNYSTKQELKGLDIVRRDWCDLAKETGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWMNSQGGRKMKAGDTVSYVICQDGSNLTASQRAYAPEQLQKQDNLTIDTQYYLAQQIHPVVARICEPIDGIDAVLIATWLGLDPTQFRVHHYHKDEENDALLGGPAQLTDEEKYRDCERFKCPCPTCGTENIYDSVLDGSGTDVEPSLYRCSNIDCKASPLTSVVQLSNKLIMDIRRCIKKYYEGWLICEEPTCQNRTRRLPLQFSRNGPLCQVCMKATLRPEYSDKSLYTQLCFYRYIFDVDCALEKLSTDQEKDKLKKVFTLKVRQDYQKLRNTAEQFLSRSGYSEVNLSKLFADCAVRP

Target ORF information:

RefSeq Version	XM_005641214.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris polymerase (DNA directed), alpha 1, catalytic subunit (POLA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641214.2

ORF Insert Sequence:

ATGGCGCTGG TACACGGCGA AGACTGCGAC CTGGGGTCGA GCGCTGTGTC AGATTCAGGG 
AGTTTTGTAT CTTCTCGATC CCGGCGAGAA AAAAAATCAA AGAGAGGGCG CCAAGAAGCT 
CTAGAAAGAC TGAAAAGGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT TGAGGACTTC 
ACAAGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCCCGCCAG 
GATGATGACT GGATCGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT 
TTTGATGATG ACCTTGAAGA CGATGCCCTT GATTCTCATG AGAAAGGAAA AGATGATAAA 
GCACGTACCA AAGACAGGAG GAATGTAAAG AAGGCTGCAG TGACAAAACC AAACAATATC 
AAGTCAATGT TCATTGCTAG TGCTGGGAAG AAGACTACAG ATAAAGCTGT AGACTTGTCC 
AAGGATGATC TGCTAGGTGA CATTCTACAA GATCTGAACA CTGAGAAACC TCCAGTAACT 
CCACCACCTA TAATGATACC AAAGAAGAAA AGATCCATTG GAGCTTCACT GAATCCTTTC 
TCTGTGCACA CCCCCAAGGC AGTTCCTTCA GGAAAGACTG CTTCCCCTGT CTCAAGGAAG 
GAGCCTCCAT TAACTCCAGT TCCTGTAAAA TGTGCTGAAT TTGCTGGAGA GCCGGTGCCA 
GCGTTGTGTA TAGATGACAA AGAGGACTCA GGAGCGATGG ATTTTGAAGA TGGTGACTTT 
GATGAGCCCA TGGAGGCTGA GGAAGTGGAT GTGGAGCCTG TGGCTGTCAA GACTTGGCAC 
CAAGAGAGTG AGCCAGCAGA GGGGGTGAAG TATGAGGCAG ATCCTGGGAA AGGGACCACG 
TCCTACTTAG GAAGTTTTCT CCCAGATGTG TCTTGTTGGG ACATTGATCA GGAAGATGAC 
AGCAGTTTCT CAGCCCAGGA AGTCCAAGTG GATTCCAGTC ACCTCCCCCT GGTGAAAGGG 
ACAGATGAGG AGCAAGTGTT TCATTTTTAT TGGCTGGATG CTTATGAAGA TCAGTACAGC 
CAACCAGGTG TGGTATTTCT GTTTGGCAAA GTTTGGATTG AATCTGCCAA AACCCATGTG 
AGCTGTTGTG TTATGGTGAA AAACATCGAG CGAACACTCT ACTTCCTTCC CCGAGAAATG 
AAAATTGATC TAAATACAGG AAAAGAAACA GAAACTCTAG TTACAATGAA GGATGTTTAT 
GATGAATTTG ATGAGAAAAT AGCAGCAAAA TATAAAATCA TGAAGTTCAA GTCTAAGCCA 
GTGGAAAAGA ATTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA 
GTTAGATACT CGGCTGAAAT GCCACAGCTT CCTCAGGATT TGAAAGGAGA AACTTTTTCT 
CATGTGTTTG GGACCAACAC ATCCAGCCTA GAAATGTTCT TGATGAACAG AAAGATCAAG 
GGACCTTGTT GGCTCGAGGT GAAAAATCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT 
AAAGTTGAGG CAATGGCCTT GAAACCAGAC CTGGTGAATG TAATTAAAGA TATTGGTCCT 
CCTCCACTCG TGGTCATGTC TTTCAGCATG AAGACAATGC AGAATGCAAA GACACATCAA 
CATGAGATTA TTGCTGTGGC AGCTTTGGTC CATCACAGCT TTGCATTAGA TAAAGCGGCC 
CCACAGCCTC CCTTCCAGTC ACACTTCTGT GTTGTATCTA AACCAAAGGA CTGTATTTTC 
CCATATACTT TCAAAGACTA CATTAAGGAA AAGAATGTGA AGGTTGAGAT TGCTGCAACA 
GAAAGAACAT TACTAGGTTT TTTCCTCGCC AAAGTTCACA AAATTGATCC TGATATCATT 
GTGGGTCATA ATATTTATGG ATTTGAACTG GAAGTGCTCC TGCAGAGAAT TAATGTATGC 
AAAGTTCCTC ACTGGTCCAA AATAGGTCGA CTGAAGCGAT CCAACATGCC GAAGCTTGGG 
GGCAGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGCC GAATGATCTG TGATGTGGAA 
ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATT TGTCTGAACT TGTTCAACAG 
ATTTTGAGAA CTGAGAGGGT TGTAATCCCA ATGGAAAATG TACGAAATAT GTACAGTGAA 
TCTTCTCATC TGCTGTACCT GTTGGAACAC ACCTGGAAAG ATGCCAGGTT CATTTTGCAG 
ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC 
ATTATGTCTA GGACGCTGAT GGGTGGACGA TCTGAGCGTA ATGAGTTCTT GTTACTTCAT 
GCGTTTTATG AAAACAACTA TATTGTGCCC GACAAGCAGA TTTTCAGAAA GCCTCAGCAA 
AAACTGGGAG ATGAAGATGA AGAACTTGAT GGAGATGCCA ATAAATACAA GAAAGGACGT 
AAGAAAGCCG CTTATGCTGG AGGCTTGGTA TTGGACCCTA AAGCTGGTTT TTATGATAAG 
TTTATTTTGC TCCTGGACTT CAACAGTCTG TATCCTTCCA TCATTCAGGA ATTCAACATT 
TGTTTTACAA CAGTGCAAAG AGTTGCTTCA GAGACACAGA AAGTTACGGA GGATGGAGAA 
CAAGAACAGA TCCCTGAGCT ACCAGACCCA GGCTTAGAAA TGGGCATTTT GCCCAGAGAG 
ATCCGGAAAC TGGTAGAGAG GAGAAAACAA GTCAAACAAC TAATGAAACA GCAAGATTTA 
AATCCAGACC TTGTTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT AACAGCAAAC 
AGTATGTATG GCTGCCTGGG ATTTTCCTAC AGCAGATTTT ATGCCAAACC ACTGGCCGCT 
CTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACAA AAGAGATGGT ACAAAAGATG 
AACCTTGAAG TTATTTATGG AGATACTGAT TCAATTATGA TAAACACCAA TAGCACCGAT 
TTGGAAGAAG TATATAAATT GGGAAACAAG GTAAAAAGTG AAGTCAACAA GTTGTACAAA 
CTGCTTGAAA TAGACATTGA CGGTGTTTTC AAGTCTCTGC TACTGCTGAA GAAAAAGAAG 
TATGGTGCTC TGGTTGTTGA GCCAACGTCA GATGGGAATT ACTCCACCAA ACAGGAACTG 
AAAGGGTTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGAAAC TGGAAACTTT 
GTCATTGGCC AGATTCTTTC TGATCAAAGC CGAGACACTA TAGTGGAAAA CATTCAGAAG 
AGGTTAATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA 
ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG TCTACCTCAT 
GTACATGTTG CCCTCTGGAT GAATTCTCAA GGAGGCAGAA AAATGAAAGC TGGAGATACT 
GTGTCATATG TCATCTGTCA GGATGGGTCA AACCTCACTG CGAGTCAGAG GGCCTATGCA 
CCTGAGCAGC TACAGAAACA GGACAATTTA ACCATTGACA CCCAGTACTA CCTGGCTCAG 
CAGATCCACC CAGTCGTGGC TCGGATCTGT GAGCCAATAG ATGGAATTGA TGCTGTCCTG 
ATTGCAACAT GGTTAGGACT CGACCCCACC CAATTTAGAG TTCATCATTA TCATAAGGAT 
GAGGAGAATG ATGCCCTACT TGGTGGCCCA GCACAGCTCA CTGATGAGGA GAAATACAGG 
GACTGTGAAA GATTCAAATG CCCATGCCCC ACATGTGGAA CAGAAAATAT ATACGATAGT 
GTCTTGGATG GTTCGGGAAC TGATGTGGAG CCCAGCTTGT ATCGCTGCAG CAACATTGAT 
TGTAAGGCTT CACCTTTGAC CTCTGTGGTA CAGCTGAGCA ACAAATTGAT CATGGACATT 
AGACGTTGCA TTAAAAAATA CTATGAAGGT TGGTTGATTT GTGAGGAGCC AACGTGTCAA 
AATCGAACTC GGCGCCTTCC CCTTCAGTTC TCCCGAAATG GGCCTCTTTG CCAAGTCTGC 
ATGAAAGCTA CACTTCGGCC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT 
TACCGGTACA TTTTTGATGT GGACTGTGCA CTGGAGAAAC TTAGTACTGA TCAGGAGAAA 
GATAAGTTGA AGAAGGTTTT TACCTTAAAA GTTCGTCAGG ACTACCAGAA ACTCAGGAAC 
ACAGCAGAGC AGTTCCTGTC CCGAAGTGGC TACTCGGAAG TGAACCTCAG CAAACTCTTC 
GCAGACTGTG CTGTGAGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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POLA1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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