Notch1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Notch1
Entrez Gene ID	18128
Full Name	notch 1
Synonyms	9930111A19Rik,Mis6,N1,Tan1,lin-12
General protein information	Preferred Names notch 1 Names neurogenic locus notch homolog protein 1 Motch A Notch gene homolog 1 mT14 major type A protein p300 transmembrane receptor Notch1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 A3\|2 18.91 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008714.3	NP_032740.3	neurogenic locus notch homolog protein 1 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NOTCH1	XP_002691666.2
Gallus gallus (chicken)	NOTCH1	XP_415420.4
Pan troglodytes (chimpanzee)	NOTCH1	XP_520371.2
Macaca mulatta (Rhesus monkey)	LOC709947	XP_002808138.1
Rattus norvegicus (Norway rat)	Notch1	NP_001099191.1
Drosophila melanogaster (fruit fly)	N	NP_001245510.1
Mus musculus (house mouse)	Notch1	NP_032740.3
Canis lupus familiaris (dog)	NOTCH1	XP_005625490.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001015	XP_309208.5
Homo sapiens (human)	NOTCH1	NP_060087.3
Danio rerio (zebrafish)	notch1b	NP_571377.2
Xenopus tropicalis (tropical clawed frog)	notch1	NP_001090757.1

Gene Ontology
Bibliography

Related articles in PubMed

Primary cilia in murine palatal rugae development.
Nakaniwa M, Kawasaki M, Kawasaki K, Yamada A, Meguro F, Takeyasu M, Ohazama A
Gene expression patterns : GEP34119062(2019 12)

Niche-Specific Factors Dynamically Regulate Sebaceous Gland Stem Cells in the Skin.
Veniaminova NA, Grachtchouk M, Doane OJ, Peterson JK, Quigley DA, Lull MV, Pyrozhenko DV, Nair RR, Patrick MT, Balmain A, Dlugosz AA, Tsoi LC, Wong SY
Developmental cell51(3)326-340.e4(2019 Nov)

A modifier in the 129S2/SvPasCrl genome is responsible for the viability of Notch1[12f/12f] mice.
Varshney S, Wei HX, Batista F, Nauman M, Sundaram S, Siminovitch K, Tanwar A, Stanley P
BMC developmental biology19(1)19(2019 10)

Altered keratinocyte differentiation is an early driver of keratin mutation-based palmoplantar keratoderma.
Zieman AG, Poll BG, Ma J, Coulombe PA
Human molecular genetics28(13)2255-2270(2019 07)

Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes.
Diéguez-Hurtado R, Kato K, Giaimo BD, Nieminen-Kelhä M, Arf H, Ferrante F, Bartkuhn M, Zimmermann T, Bixel MG, Eilken HM, Adams S, Borggrefe T, Vajkoczy P, Adams RH
Nature communications10(1)2817(2019 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Notch1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Notch1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22855	NM_008714.3 Latest version!	Mus musculus notch 1 (Notch1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Notch1 ( NM_008714.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032740.3 Mus musculus notch 1 (Notch1), mRNA.

Price & Availability↑

CloneID	OMu22855
Clone ID Related Accession (Same CDS sequence)	NM_008714.3
Accession Version	NM_008714.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Mus musculus(house mouse)
Product	neurogenic locus notch homolog protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY233933.1, BC138441.1 and BF318436.1. On Mar 13, 2009 this sequence version replaced NM_008714.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK090118.1, AF508809.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCACGGC TCCTGACGCC CTTGCTCTGC CTAACGCTGC TGCCCGCGCT CGCCGCAAGA 
GGCTTGAGAT GCTCCCAGCC AAGTGGGACC TGCCTGAATG GAGGGAGGTG CGAAGTGGCC 
AACGGCACTG AAGCCTGTGT CTGCAGCGGA GCCTTTGTGG GCCAACGATG CCAGGACTCC 
AATCCTTGCC TCAGCACACC GTGTAAGAAT GCTGGAACGT GCCACGTTGT GGACCATGGT 
GGCACTGTGG ATTATGCCTG CAGCTGTCCC CTGGGTTTCT CTGGGCCCCT CTGCCTGACA 
CCTCTGGACA ACGCCTGCCT GGCCAACCCC TGCCGCAATG GGGGCACCTG TGACCTGCTC 
ACTCTCACAG AGTACAAGTG CCGCTGCCCA CCAGGGTGGT CAGGAAAATC ATGTCAGCAG 
GCTGACCCCT GTGCCTCCAA CCCCTGTGCC AATGGTGGCC AGTGCCTGCC CTTTGAGTCT 
TCATACATCT GTCGCTGCCC GCCTGGCTTC CATGGCCCCA CCTGCAGGCA AGATGTTAAT 
GAGTGCAGCC AGAACCCTGG GCTGTGCCGC CATGGAGGCA CCTGCCACAA TGAGATCGGC 
TCCTATCGCT GTGCCTGCCG TGCCACCCAT ACTGGTCCCC ACTGTGAACT GCCCTATGTG 
CCCTGCAGCC CCTCACCCTG CCAGAATGGA GGCACCTGCC GTCCTACAGG GGACACCACC 
CACGAGTGTG CCTGCTTGCC AGGTTTTGCT GGACAGAACT GTGAAGAAAA TGTGGATGAC 
TGTCCAGGAA ACAACTGCAA GAATGGGGGT GCCTGTGTGG ACGGCGTGAA TACCTACAAT 
TGCCGCTGCC CACCGGAGTG GACGGGTCAG TACTGTACAG AGGATGTGGA CGAATGTCAG 
CTCATGCCCA ATGCCTGCCA GAATGGCGGA ACCTGCCACA ACACACACGG CGGCTACAAC 
TGTGTGTGTG TCAATGGGTG GACTGGCGAG GACTGCAGTG AGAACATTGA TGACTGTGCC 
AGTGCCGCCT GTTTCCAGGG TGCCACTTGC CACGACCGTG TGGCTTCCTT CTACTGCGAA 
TGTCCGCATG GGCGCACAGG TCTGCTGTGC CACCTCAACG ATGCGTGCAT CAGCAACCCC 
TGCAACGAGG GCTCCAACTG TGACACCAAC CCTGTCAACG GCAAAGCCAT CTGCACCTGC 
CCCTCGGGGT ACACAGGGCC AGCCTGCAGC CAGGACGTGG ATGAGTGTGC TCTGGGTGCC 
AACCCTTGTG AGCACGCAGG CAAATGCCTC AACACACTGG GTTCTTTTGA GTGCCAGTGT 
CTACAGGGCT ACACGGGACC CCGCTGTGAG ATTGATGTTA ATGAGTGCAT CTCCAACCCA 
TGTCAGAATG ATGCCACTTG CCTGGACCAG ATTGGGGAGT TCCAATGCAT ATGTATGCCA 
GGTTATGAAG GTGTATACTG TGAAATCAAC ACGGATGAGT GCGCCAGCAG CCCCTGTCTG 
CACAATGGCC ACTGCATGGA CAAGATCAAT GAGTTCCAAT GTCAGTGCCC CAAAGGCTTC 
AACGGGCACC TGTGCCAGTA TGATGTGGAT GAGTGTGCCA GCACACCATG CAAGAACGGT 
GCCAAGTGCC TGGATGGGCC CAACACCTAT ACCTGCGTGT GTACAGAAGG TTACACAGGG 
ACCCACTGCG AAGTGGACAT TGACGAGTGT GACCCTGACC CCTGCCACTA TGGTTCCTGT 
AAGGATGGTG TGGCCACCTT TACCTGCCTG TGCCAGCCAG GCTACACAGG CCATCACTGT 
GAGACCAACA TCAATGAGTG CCACAGCCAA CCGTGCCGCC ATGGGGGCAC CTGCCAGGAC 
CGTGACAACT CCTACCTCTG CTTATGCCTC AAGGGAACCA CAGGGCCCAA CTGTGAGATC 
AACCTGGATG ACTGCGCCAG CAACCCCTGT GACTCTGGCA CCTGTCTGGA CAAGATTGAT 
GGCTACGAAT GTGCCTGTGA ACCAGGCTAC ACAGGAAGCA TGTGTAACGT CAACATTGAC 
GAATGTGCGG GCAGCCCCTG CCACAACGGG GGCACTTGTG AGGATGGCAT CGCGGGCTTC 
ACTTGCCGCT GCCCCGAGGG CTACCATGAC CCCACGTGCC TGTCCGAGGT CAACGAGTGC 
AACAGTAACC CCTGCATCCA CGGAGCTTGC CGGGATGGCC TCAATGGGTA CAAGTGTGAC 
TGTGCCCCTG GGTGGAGTGG AACAAACTGT GACATCAACA ACAACGAGTG TGAGTCCAAC 
CCTTGTGTCA ACGGTGGCAC CTGCAAGGAC ATGACCAGTG GCTACGTATG CACCTGCCGA 
GAAGGCTTCA GTGGCCCTAA TTGCCAGACC AACATCAACG AATGTGCCTC CAACCCCTGC 
CTGAACCAGG GGACCTGCAT TGATGATGTC GCTGGATACA AGTGCAACTG TCCTCTGCCA 
TATACAGGAG CCACGTGTGA GGTGGTGTTG GCCCCATGTG CTACCAGCCC CTGCAAAAAC 
AGCGGGGTAT GCAAGGAGTC TGAAGACTAT GAGAGTTTTT CCTGTGTCTG TCCCACAGGC 
TGGCAAGGTC AAACCTGCGA GGTTGACATC AATGAGTGTG TGAAAAGCCC ATGTCGCCAT 
GGGGCCTCCT GCCAGAACAC CAATGGCAGC TACCGCTGCC TCTGCCAGGC CGGCTATACA 
GGTCGCAACT GTGAGAGTGA CATCGATGAC TGCCGCCCCA ACCCGTGTCA CAATGGGGGT 
TCCTGCACCG ATGGCATCAA CACAGCCTTC TGCGACTGCC TGCCCGGCTT CCAGGGTGCC 
TTCTGTGAGG AGGACATCAA TGAATGTGCC AGCAATCCCT GCCAAAATGG TGCCAATTGC 
ACTGACTGTG TGGACAGCTA CACATGTACC TGCCCCGTGG GCTTCAATGG CATCCACTGC 
GAGAACAACA CACCTGACTG TACTGAGAGC TCCTGCTTCA ATGGTGGTAC CTGTGTGGAT 
GGTATCAACT CCTTCACCTG TCTGTGTCCA CCTGGCTTCA CGGGCAGCTA CTGTCAGTAT 
GATGTCAATG AGTGTGATTC ACGGCCCTGT CTGCACGGTG GTACCTGCCA AGACAGCTAT 
GGTACTTATA AGTGTACCTG CCCACAGGGC TACACTGGTC TCAACTGCCA GAACCTTGTG 
CGCTGGTGCG ACTCGGCTCC CTGCAAGAAT GGTGGCAGGT GCTGGCAGAC CAACACGCAG 
TACCACTGTG AGTGCCGCAG CGGCTGGACT GGCGTCAACT GCGACGTGCT CAGTGTGTCC 
TGTGAGGTGG CTGCACAGAA GCGAGGCATT GACGTCACTC TCCTGTGCCA GCATGGAGGG 
CTCTGTGTGG ATGAGGGAGA TAAACATTAC TGCCACTGCC AGGCAGGCTA CACGGGCAGC 
TACTGTGAGG ACGAGGTGGA CGAGTGCTCA CCTAACCCCT GCCAGAATGG AGCTACCTGC 
ACTGACTATC TCGGCGGCTT TTCCTGCAAG TGTGTGGCTG GCTACCATGG GTCTAACTGC 
TCCGAGGAGA TCAACGAGTG CCTGTCCCAG CCCTGCCAGA ATGGGGGTAC CTGCATTGAT 
CTGACCAACT CCTACAAGTG TTCCTGCCCC CGGGGGACAC AGGGTGTACA CTGTGAGATC 
AATGTTGATG ACTGCCATCC CCCCCTTGAC CCTGCCTCCC GAAGCCCCAA GTGCTTCAAC 
AATGGCACCT GTGTGGACCA GGTGGGTGGC TATACCTGCA CCTGCCCACC AGGCTTCGTC 
GGGGAGCGGT GTGAGGGTGA TGTCAATGAA TGTCTCTCCA ACCCCTGTGA CCCACGTGGC 
ACCCAGAACT GTGTGCAGCG TGTTAATGAC TTCCACTGCG AGTGCCGGGC TGGCCACACT 
GGACGCCGCT GTGAGTCAGT CATCAATGGC TGCAGGGGCA AACCTTGCAA GAATGGGGGT 
GTCTGTGCCG TGGCCTCCAA CACCGCCCGT GGATTCATCT GTAGGTGCCC TGCGGGCTTC 
GAGGGTGCCA CATGTGAGAA TGATGCCCGC ACTTGTGGCA GCTTACGCTG CCTCAACGGT 
GGTACATGCA TCTCGGGCCC ACGTAGTCCC ACCTGCCTAT GCCTGGGATC CTTCACCGGC 
CCTGAGTGCC AGTTCCCAGC CAGCAGCCCC TGTGTGGGTA GCAACCCCTG CTACAATCAG 
GGCACCTGTG AGCCCACATC CGAGAACCCT TTCTACCGCT GTCTATGCCC TGCCAAATTC 
AACGGGCTAC TGTGCCACAT CCTGGACTAC AGCTTCACAG GTGGCGCTGG GCGCGACATT 
CCCCCACCGC AGATTGAGGA GGCCTGTGAG CTGCCTGAGT GCCAGGTGGA TGCAGGCAAT 
AAGGTCTGCA ACCTGCAGTG TAATAATCAC GCATGTGGCT GGGATGGTGG CGACTGCTCC 
CTCAACTTCA ATGACCCCTG GAAGAACTGC ACGCAGTCTC TACAGTGCTG GAAGTATTTT 
AGCGACGGCC ACTGTGACAG CCAGTGCAAC TCGGCCGGCT GCCTCTTTGA TGGCTTCGAC 
TGCCAGCTCA CCGAGGGACA GTGCAACCCC CTGTATGACC AGTACTGCAA GGACCACTTC 
AGTGATGGCC ACTGCGACCA GGGCTGTAAC AGTGCCGAAT GTGAGTGGGA TGGCCTAGAC 
TGTGCTGAGC ATGTACCCGA GCGGCTGGCA GCCGGCACCC TGGTGCTGGT GGTGCTGCTT 
CCACCCGACC AGCTACGGAA CAACTCCTTC CACTTTCTGC GGGAGCTCAG CCACGTGCTG 
CACACCAACG TGGTCTTCAA GCGTGATGCG CAAGGCCAGC AGATGATCTT CCCGTACTAT 
GGCCACGAGG AAGAGCTGCG CAAGCACCCA ATCAAGCGCT CTACAGTGGG TTGGGCCACC 
TCTTCACTGC TTCCTGGTAC CAGTGGTGGG CGCCAGCGCA GGGAGCTGGA CCCCATGGAC 
ATCCGTGGCT CCATTGTCTA CCTGGAGATC GACAACCGGC AATGTGTGCA GTCATCCTCG 
CAGTGCTTCC AGAGTGCCAC CGATGTGGCT GCCTTCCTAG GTGCTCTTGC GTCACTTGGC 
AGCCTCAATA TTCCTTACAA GATTGAGGCC GTGAAGAGTG AGCCGGTGGA GCCTCCGCTG 
CCCTCGCAGC TGCACCTCAT GTACGTGGCA GCGGCCGCCT TCGTGCTCCT GTTCTTTGTG 
GGCTGTGGGG TGCTGCTGTC CCGCAAGCGC CGGCGGCAGC ATGGCCAGCT CTGGTTCCCT 
GAGGGTTTCA AAGTGTCAGA GGCCAGCAAG AAGAAGCGGA GAGAGCCCCT CGGCGAGGAC 
TCAGTCGGCC TCAAGCCCCT GAAGAATGCC TCAGATGGTG CTCTGATGGA CGACAATCAG 
AACGAGTGGG GAGACGAAGA CCTGGAGACC AAGAAGTTCC GGTTTGAGGA GCCAGTAGTT 
CTCCCTGACC TGAGTGATCA GACTGACCAC AGGCAGTGGA CCCAGCAGCA CCTGGACGCT 
GCTGACCTGC GCATGTCTGC CATGGCCCCA ACACCGCCTC AGGGGGAGGT GGATGCTGAC 
TGCATGGATG TCAATGTTCG AGGACCAGAT GGCTTCACAC CCCTCATGAT TGCCTCCTGC 
AGTGGAGGGG GCCTTGAGAC AGGCAACAGT GAAGAAGAAG AAGATGCACC TGCTGTCATC 
TCTGACTTCA TCTACCAGGG CGCCAGCTTG CACAACCAGA CAGACCGCAC CGGGGAGACC 
GCCTTGCACT TGGCTGCCCG ATACTCTCGT TCAGATGCTG CAAAGCGCTT GCTGGAGGCC 
AGTGCAGATG CCAACATCCA GGACAACATG GGCCGTACTC CGTTACATGC AGCAGTTTCT 
GCAGATGCTC AGGGTGTCTT CCAGATCCTG CTCCGGAACA GGGCCACAGA TCTGGATGCC 
CGAATGCATG ATGGCACAAC TCCACTGATC CTGGCTGCGC GCCTGGCCGT GGAGGGCATG 
CTGGAGGACC TCATCAACTC ACATGCTGAC GTCAATGCCG TGGATGACCT AGGCAAGTCG 
GCTTTGCATT GGGCGGCCGC GGTGAACAAT GTGGATGCTG CTGTTGTGCT CCTGAAGAAC 
GGAGCCAACA AGGACATGCA GAACAACAAG GAGGAGACTC CCCTGTTCCT GGCCGCCCGT 
GAGGGCAGCT ATGAGACTGC CAAAGTGTTG CTGGACCACT TTGCCAACCG GGACATCACG 
GATCACATGG ACCGATTGCC GCGGGACATC GCACAGGAGC GTATGCACCA CGATATCGTG 
CGGCTTTTGG ATGAGTACAA CCTGGTGCGC AGCCCACAGC TGCATGGCAC TGCCCTGGGT 
GGCACACCCA CTCTGTCTCC CACACTCTGC TCGCCCAATG GCTACCTGGG CAATCTCAAG 
TCTGCCACAC AGGGCAAGAA GGCCCGCAAG CCCAGCACCA AAGGGCTGGC TTGTGGTAGC 
AAGGAAGCTA AGGACCTCAA GGCACGGAGG AAGAAGTCCC AGGATGGCAA GGGCTGCCTG 
TTGGACAGCT CGAGCATGCT GTCGCCTGTG GACTCCCTCG AGTCACCCCA TGGCTACTTG 
TCAGATGTGG CCTCGCCACC CCTCCTCCCC TCCCCATTCC AGCAGTCTCC ATCCATGCCT 
CTCAGCCACC TGCCTGGTAT GCCTGACACT CACCTGGGCA TCAGCCACTT GAATGTGGCA 
GCCAAGCCTG AGATGGCAGC ACTGGCTGGA GGTAGCCGGT TGGCCTTTGA GCCACCCCCG 
CCACGCCTCT CCCACCTGCC TGTAGCCTCC AGTGCCAGCA CAGTGCTGAG TACCAATGGC 
ACGGGGGCTA TGAATTTCAC CGTGGGTGCA CCGGCAAGCT TGAATGGCCA GTGTGAGTGG 
CTTCCCCGGC TCCAGAATGG CATGGTGCCC AGCCAGTACA ACCCACTACG GCCGGGTGTG 
ACGCCGGGCA CACTGAGCAC ACAGGCAGCT GGCCTCCAGC ATAGCATGAT GGGGCCACTA 
CACAGCAGCC TCTCCACCAA TACCTTGTCC CCGATTATTT ACCAGGGCCT GCCCAACACA 
CGGCTGGCAA CACAGCCTCA CCTGGTGCAG ACCCAGCAGG TGCAGCCACA GAACTTACAG 
CTCCAGCCTC AGAACCTGCA GCCACCATCA CAGCCACACC TCAGTGTGAG CTCGGCAGCC 
AATGGGCACC TGGGCCGGAG CTTCTTGAGT GGGGAGCCCA GTCAGGCAGA TGTACAACCG 
CTGGGCCCCA GCAGTCTGCC TGTGCACACC ATTCTGCCCC AGGAAAGCCA GGCCCTGCCC 
ACATCACTGC CATCCTCCAT GGTCCCACCC ATGACCACTA CCCAGTTCCT GACCCCTCCT 
TCCCAGCACA GTTACTCCTC CTCCCCTGTG GACAACACCC CCAGCCACCA GCTGCAGGTG 
CCAGAGCACC CCTTCCTCAC CCCATCCCCT GAGTCCCCTG ACCAGTGGTC CAGCTCCTCC 
CCGCATTCCA ACATCTCTGA TTGGTCCGAG GGCATCTCCA GCCCGCCCAC CACCATGCCG 
TCCCAGATCA CCCACATTCC AGAGGCATTT AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032740.3
CDS	265..7860
Translation	MPRLLTPLLCLTLLPALAARGLRCSQPSGTCLNGGRCEVANGTEACVCSGAFVGQRCQDSNPCLSTPCKNAGTCHVVDHGGTVDYACSCPLGFSGPLCLTPLDNACLANPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFESSYICRCPPGFHGPTCRQDVNECSQNPGLCRHGGTCHNEIGSYRCACRATHTGPHCELPYVPCSPSPCQNGGTCRPTGDTTHECACLPGFAGQNCEENVDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFQGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECALGANPCEHAGKCLNTLGSFECQCLQGYTGPRCEIDVNECISNPCQNDATCLDQIGEFQCICMPGYEGVYCEINTDECASSPCLHNGHCMDKINEFQCQCPKGFNGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGTHCEVDIDECDPDPCHYGSCKDGVATFTCLCQPGYTGHHCETNINECHSQPCRHGGTCQDRDNSYLCLCLKGTTGPNCEINLDDCASNPCDSGTCLDKIDGYECACEPGYTGSMCNVNIDECAGSPCHNGGTCEDGIAGFTCRCPEGYHDPTCLSEVNECNSNPCIHGACRDGLNGYKCDCAPGWSGTNCDINNNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCPLPYTGATCEVVLAPCATSPCKNSGVCKESEDYESFSCVCPTGWQGQTCEVDINECVKSPCRHGASCQNTNGSYRCLCQAGYTGRNCESDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFQGAFCEEDINECASNPCQNGANCTDCVDSYTCTCPVGFNGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQYDVNECDSRPCLHGGTCQDSYGTYKCTCPQGYTGLNCQNLVRWCDSAPCKNGGRCWQTNTQYHCECRSGWTGVNCDVLSVSCEVAAQKRGIDVTLLCQHGGLCVDEGDKHYCHCQAGYTGSYCEDEVDECSPNPCQNGATCTDYLGGFSCKCVAGYHGSNCSEEINECLSQPCQNGGTCIDLTNSYKCSCPRGTQGVHCEINVDDCHPPLDPASRSPKCFNNGTCVDQVGGYTCTCPPGFVGERCEGDVNECLSNPCDPRGTQNCVQRVNDFHCECRAGHTGRRCESVINGCRGKPCKNGGVCAVASNTARGFICRCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGSFTGPECQFPASSPCVGSNPCYNQGTCEPTSENPFYRCLCPAKFNGLLCHILDYSFTGGAGRDIPPPQIEEACELPECQVDAGNKVCNLQCNNHACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQLTEGQCNPLYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVLVVLLPPDQLRNNSFHFLRELSHVLHTNVVFKRDAQGQQMIFPYYGHEEELRKHPIKRSTVGWATSSLLPGTSGGRQRRELDPMDIRGSIVYLEIDNRQCVQSSSQCFQSATDVAAFLGALASLGSLNIPYKIEAVKSEPVEPPLPSQLHLMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLSDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILLRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNKEETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIVRLLDEYNLVRSPQLHGTALGGTPTLSPTLCSPNGYLGNLKSATQGKKARKPSTKGLACGSKEAKDLKARRKKSQDGKGCLLDSSSMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSMPLSHLPGMPDTHLGISHLNVAAKPEMAALAGGSRLAFEPPPPRLSHLPVASSASTVLSTNGTGAMNFTVGAPASLNGQCEWLPRLQNGMVPSQYNPLRPGVTPGTLSTQAAGLQHSMMGPLHSSLSTNTLSPIIYQGLPNTRLATQPHLVQTQQVQPQNLQLQPQNLQPPSQPHLSVSSAANGHLGRSFLSGEPSQADVQPLGPSSLPVHTILPQESQALPTSLPSSMVPPMTTTQFLTPPSQHSYSSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNISDWSEGISSPPTTMPSQITHIPEAFK

Target ORF information:

RefSeq Version	NM_008714.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus notch 1 (Notch1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008714.3

ORF Insert Sequence:

ATGCCACGGC TCCTGACGCC CTTGCTCTGC CTAACGCTGC TGCCCGCGCT CGCCGCAAGA 
GGCTTGAGAT GCTCCCAGCC AAGTGGGACC TGCCTGAATG GAGGGAGGTG CGAAGTGGCC 
AACGGCACTG AAGCCTGTGT CTGCAGCGGA GCCTTTGTGG GCCAACGATG CCAGGACTCC 
AATCCTTGCC TCAGCACACC GTGTAAGAAT GCTGGAACGT GCCACGTTGT GGACCATGGT 
GGCACTGTGG ATTATGCCTG CAGCTGTCCC CTGGGTTTCT CTGGGCCCCT CTGCCTGACA 
CCTCTGGACA ACGCCTGCCT GGCCAACCCC TGCCGCAATG GGGGCACCTG TGACCTGCTC 
ACTCTCACAG AGTACAAGTG CCGCTGCCCA CCAGGGTGGT CAGGAAAATC ATGTCAGCAG 
GCTGACCCCT GTGCCTCCAA CCCCTGTGCC AATGGTGGCC AGTGCCTGCC CTTTGAGTCT 
TCATACATCT GTCGCTGCCC GCCTGGCTTC CATGGCCCCA CCTGCAGGCA AGATGTTAAT 
GAGTGCAGCC AGAACCCTGG GCTGTGCCGC CATGGAGGCA CCTGCCACAA TGAGATCGGC 
TCCTATCGCT GTGCCTGCCG TGCCACCCAT ACTGGTCCCC ACTGTGAACT GCCCTATGTG 
CCCTGCAGCC CCTCACCCTG CCAGAATGGA GGCACCTGCC GTCCTACAGG GGACACCACC 
CACGAGTGTG CCTGCTTGCC AGGTTTTGCT GGACAGAACT GTGAAGAAAA TGTGGATGAC 
TGTCCAGGAA ACAACTGCAA GAATGGGGGT GCCTGTGTGG ACGGCGTGAA TACCTACAAT 
TGCCGCTGCC CACCGGAGTG GACGGGTCAG TACTGTACAG AGGATGTGGA CGAATGTCAG 
CTCATGCCCA ATGCCTGCCA GAATGGCGGA ACCTGCCACA ACACACACGG CGGCTACAAC 
TGTGTGTGTG TCAATGGGTG GACTGGCGAG GACTGCAGTG AGAACATTGA TGACTGTGCC 
AGTGCCGCCT GTTTCCAGGG TGCCACTTGC CACGACCGTG TGGCTTCCTT CTACTGCGAA 
TGTCCGCATG GGCGCACAGG TCTGCTGTGC CACCTCAACG ATGCGTGCAT CAGCAACCCC 
TGCAACGAGG GCTCCAACTG TGACACCAAC CCTGTCAACG GCAAAGCCAT CTGCACCTGC 
CCCTCGGGGT ACACAGGGCC AGCCTGCAGC CAGGACGTGG ATGAGTGTGC TCTGGGTGCC 
AACCCTTGTG AGCACGCAGG CAAATGCCTC AACACACTGG GTTCTTTTGA GTGCCAGTGT 
CTACAGGGCT ACACGGGACC CCGCTGTGAG ATTGATGTTA ATGAGTGCAT CTCCAACCCA 
TGTCAGAATG ATGCCACTTG CCTGGACCAG ATTGGGGAGT TCCAATGCAT ATGTATGCCA 
GGTTATGAAG GTGTATACTG TGAAATCAAC ACGGATGAGT GCGCCAGCAG CCCCTGTCTG 
CACAATGGCC ACTGCATGGA CAAGATCAAT GAGTTCCAAT GTCAGTGCCC CAAAGGCTTC 
AACGGGCACC TGTGCCAGTA TGATGTGGAT GAGTGTGCCA GCACACCATG CAAGAACGGT 
GCCAAGTGCC TGGATGGGCC CAACACCTAT ACCTGCGTGT GTACAGAAGG TTACACAGGG 
ACCCACTGCG AAGTGGACAT TGACGAGTGT GACCCTGACC CCTGCCACTA TGGTTCCTGT 
AAGGATGGTG TGGCCACCTT TACCTGCCTG TGCCAGCCAG GCTACACAGG CCATCACTGT 
GAGACCAACA TCAATGAGTG CCACAGCCAA CCGTGCCGCC ATGGGGGCAC CTGCCAGGAC 
CGTGACAACT CCTACCTCTG CTTATGCCTC AAGGGAACCA CAGGGCCCAA CTGTGAGATC 
AACCTGGATG ACTGCGCCAG CAACCCCTGT GACTCTGGCA CCTGTCTGGA CAAGATTGAT 
GGCTACGAAT GTGCCTGTGA ACCAGGCTAC ACAGGAAGCA TGTGTAACGT CAACATTGAC 
GAATGTGCGG GCAGCCCCTG CCACAACGGG GGCACTTGTG AGGATGGCAT CGCGGGCTTC 
ACTTGCCGCT GCCCCGAGGG CTACCATGAC CCCACGTGCC TGTCCGAGGT CAACGAGTGC 
AACAGTAACC CCTGCATCCA CGGAGCTTGC CGGGATGGCC TCAATGGGTA CAAGTGTGAC 
TGTGCCCCTG GGTGGAGTGG AACAAACTGT GACATCAACA ACAACGAGTG TGAGTCCAAC 
CCTTGTGTCA ACGGTGGCAC CTGCAAGGAC ATGACCAGTG GCTACGTATG CACCTGCCGA 
GAAGGCTTCA GTGGCCCTAA TTGCCAGACC AACATCAACG AATGTGCCTC CAACCCCTGC 
CTGAACCAGG GGACCTGCAT TGATGATGTC GCTGGATACA AGTGCAACTG TCCTCTGCCA 
TATACAGGAG CCACGTGTGA GGTGGTGTTG GCCCCATGTG CTACCAGCCC CTGCAAAAAC 
AGCGGGGTAT GCAAGGAGTC TGAAGACTAT GAGAGTTTTT CCTGTGTCTG TCCCACAGGC 
TGGCAAGGTC AAACCTGCGA GGTTGACATC AATGAGTGTG TGAAAAGCCC ATGTCGCCAT 
GGGGCCTCCT GCCAGAACAC CAATGGCAGC TACCGCTGCC TCTGCCAGGC CGGCTATACA 
GGTCGCAACT GTGAGAGTGA CATCGATGAC TGCCGCCCCA ACCCGTGTCA CAATGGGGGT 
TCCTGCACCG ATGGCATCAA CACAGCCTTC TGCGACTGCC TGCCCGGCTT CCAGGGTGCC 
TTCTGTGAGG AGGACATCAA TGAATGTGCC AGCAATCCCT GCCAAAATGG TGCCAATTGC 
ACTGACTGTG TGGACAGCTA CACATGTACC TGCCCCGTGG GCTTCAATGG CATCCACTGC 
GAGAACAACA CACCTGACTG TACTGAGAGC TCCTGCTTCA ATGGTGGTAC CTGTGTGGAT 
GGTATCAACT CCTTCACCTG TCTGTGTCCA CCTGGCTTCA CGGGCAGCTA CTGTCAGTAT 
GATGTCAATG AGTGTGATTC ACGGCCCTGT CTGCACGGTG GTACCTGCCA AGACAGCTAT 
GGTACTTATA AGTGTACCTG CCCACAGGGC TACACTGGTC TCAACTGCCA GAACCTTGTG 
CGCTGGTGCG ACTCGGCTCC CTGCAAGAAT GGTGGCAGGT GCTGGCAGAC CAACACGCAG 
TACCACTGTG AGTGCCGCAG CGGCTGGACT GGCGTCAACT GCGACGTGCT CAGTGTGTCC 
TGTGAGGTGG CTGCACAGAA GCGAGGCATT GACGTCACTC TCCTGTGCCA GCATGGAGGG 
CTCTGTGTGG ATGAGGGAGA TAAACATTAC TGCCACTGCC AGGCAGGCTA CACGGGCAGC 
TACTGTGAGG ACGAGGTGGA CGAGTGCTCA CCTAACCCCT GCCAGAATGG AGCTACCTGC 
ACTGACTATC TCGGCGGCTT TTCCTGCAAG TGTGTGGCTG GCTACCATGG GTCTAACTGC 
TCCGAGGAGA TCAACGAGTG CCTGTCCCAG CCCTGCCAGA ATGGGGGTAC CTGCATTGAT 
CTGACCAACT CCTACAAGTG TTCCTGCCCC CGGGGGACAC AGGGTGTACA CTGTGAGATC 
AATGTTGATG ACTGCCATCC CCCCCTTGAC CCTGCCTCCC GAAGCCCCAA GTGCTTCAAC 
AATGGCACCT GTGTGGACCA GGTGGGTGGC TATACCTGCA CCTGCCCACC AGGCTTCGTC 
GGGGAGCGGT GTGAGGGTGA TGTCAATGAA TGTCTCTCCA ACCCCTGTGA CCCACGTGGC 
ACCCAGAACT GTGTGCAGCG TGTTAATGAC TTCCACTGCG AGTGCCGGGC TGGCCACACT 
GGACGCCGCT GTGAGTCAGT CATCAATGGC TGCAGGGGCA AACCTTGCAA GAATGGGGGT 
GTCTGTGCCG TGGCCTCCAA CACCGCCCGT GGATTCATCT GTAGGTGCCC TGCGGGCTTC 
GAGGGTGCCA CATGTGAGAA TGATGCCCGC ACTTGTGGCA GCTTACGCTG CCTCAACGGT 
GGTACATGCA TCTCGGGCCC ACGTAGTCCC ACCTGCCTAT GCCTGGGATC CTTCACCGGC 
CCTGAGTGCC AGTTCCCAGC CAGCAGCCCC TGTGTGGGTA GCAACCCCTG CTACAATCAG 
GGCACCTGTG AGCCCACATC CGAGAACCCT TTCTACCGCT GTCTATGCCC TGCCAAATTC 
AACGGGCTAC TGTGCCACAT CCTGGACTAC AGCTTCACAG GTGGCGCTGG GCGCGACATT 
CCCCCACCGC AGATTGAGGA GGCCTGTGAG CTGCCTGAGT GCCAGGTGGA TGCAGGCAAT 
AAGGTCTGCA ACCTGCAGTG TAATAATCAC GCATGTGGCT GGGATGGTGG CGACTGCTCC 
CTCAACTTCA ATGACCCCTG GAAGAACTGC ACGCAGTCTC TACAGTGCTG GAAGTATTTT 
AGCGACGGCC ACTGTGACAG CCAGTGCAAC TCGGCCGGCT GCCTCTTTGA TGGCTTCGAC 
TGCCAGCTCA CCGAGGGACA GTGCAACCCC CTGTATGACC AGTACTGCAA GGACCACTTC 
AGTGATGGCC ACTGCGACCA GGGCTGTAAC AGTGCCGAAT GTGAGTGGGA TGGCCTAGAC 
TGTGCTGAGC ATGTACCCGA GCGGCTGGCA GCCGGCACCC TGGTGCTGGT GGTGCTGCTT 
CCACCCGACC AGCTACGGAA CAACTCCTTC CACTTTCTGC GGGAGCTCAG CCACGTGCTG 
CACACCAACG TGGTCTTCAA GCGTGATGCG CAAGGCCAGC AGATGATCTT CCCGTACTAT 
GGCCACGAGG AAGAGCTGCG CAAGCACCCA ATCAAGCGCT CTACAGTGGG TTGGGCCACC 
TCTTCACTGC TTCCTGGTAC CAGTGGTGGG CGCCAGCGCA GGGAGCTGGA CCCCATGGAC 
ATCCGTGGCT CCATTGTCTA CCTGGAGATC GACAACCGGC AATGTGTGCA GTCATCCTCG 
CAGTGCTTCC AGAGTGCCAC CGATGTGGCT GCCTTCCTAG GTGCTCTTGC GTCACTTGGC 
AGCCTCAATA TTCCTTACAA GATTGAGGCC GTGAAGAGTG AGCCGGTGGA GCCTCCGCTG 
CCCTCGCAGC TGCACCTCAT GTACGTGGCA GCGGCCGCCT TCGTGCTCCT GTTCTTTGTG 
GGCTGTGGGG TGCTGCTGTC CCGCAAGCGC CGGCGGCAGC ATGGCCAGCT CTGGTTCCCT 
GAGGGTTTCA AAGTGTCAGA GGCCAGCAAG AAGAAGCGGA GAGAGCCCCT CGGCGAGGAC 
TCAGTCGGCC TCAAGCCCCT GAAGAATGCC TCAGATGGTG CTCTGATGGA CGACAATCAG 
AACGAGTGGG GAGACGAAGA CCTGGAGACC AAGAAGTTCC GGTTTGAGGA GCCAGTAGTT 
CTCCCTGACC TGAGTGATCA GACTGACCAC AGGCAGTGGA CCCAGCAGCA CCTGGACGCT 
GCTGACCTGC GCATGTCTGC CATGGCCCCA ACACCGCCTC AGGGGGAGGT GGATGCTGAC 
TGCATGGATG TCAATGTTCG AGGACCAGAT GGCTTCACAC CCCTCATGAT TGCCTCCTGC 
AGTGGAGGGG GCCTTGAGAC AGGCAACAGT GAAGAAGAAG AAGATGCACC TGCTGTCATC 
TCTGACTTCA TCTACCAGGG CGCCAGCTTG CACAACCAGA CAGACCGCAC CGGGGAGACC 
GCCTTGCACT TGGCTGCCCG ATACTCTCGT TCAGATGCTG CAAAGCGCTT GCTGGAGGCC 
AGTGCAGATG CCAACATCCA GGACAACATG GGCCGTACTC CGTTACATGC AGCAGTTTCT 
GCAGATGCTC AGGGTGTCTT CCAGATCCTG CTCCGGAACA GGGCCACAGA TCTGGATGCC 
CGAATGCATG ATGGCACAAC TCCACTGATC CTGGCTGCGC GCCTGGCCGT GGAGGGCATG 
CTGGAGGACC TCATCAACTC ACATGCTGAC GTCAATGCCG TGGATGACCT AGGCAAGTCG 
GCTTTGCATT GGGCGGCCGC GGTGAACAAT GTGGATGCTG CTGTTGTGCT CCTGAAGAAC 
GGAGCCAACA AGGACATGCA GAACAACAAG GAGGAGACTC CCCTGTTCCT GGCCGCCCGT 
GAGGGCAGCT ATGAGACTGC CAAAGTGTTG CTGGACCACT TTGCCAACCG GGACATCACG 
GATCACATGG ACCGATTGCC GCGGGACATC GCACAGGAGC GTATGCACCA CGATATCGTG 
CGGCTTTTGG ATGAGTACAA CCTGGTGCGC AGCCCACAGC TGCATGGCAC TGCCCTGGGT 
GGCACACCCA CTCTGTCTCC CACACTCTGC TCGCCCAATG GCTACCTGGG CAATCTCAAG 
TCTGCCACAC AGGGCAAGAA GGCCCGCAAG CCCAGCACCA AAGGGCTGGC TTGTGGTAGC 
AAGGAAGCTA AGGACCTCAA GGCACGGAGG AAGAAGTCCC AGGATGGCAA GGGCTGCCTG 
TTGGACAGCT CGAGCATGCT GTCGCCTGTG GACTCCCTCG AGTCACCCCA TGGCTACTTG 
TCAGATGTGG CCTCGCCACC CCTCCTCCCC TCCCCATTCC AGCAGTCTCC ATCCATGCCT 
CTCAGCCACC TGCCTGGTAT GCCTGACACT CACCTGGGCA TCAGCCACTT GAATGTGGCA 
GCCAAGCCTG AGATGGCAGC ACTGGCTGGA GGTAGCCGGT TGGCCTTTGA GCCACCCCCG 
CCACGCCTCT CCCACCTGCC TGTAGCCTCC AGTGCCAGCA CAGTGCTGAG TACCAATGGC 
ACGGGGGCTA TGAATTTCAC CGTGGGTGCA CCGGCAAGCT TGAATGGCCA GTGTGAGTGG 
CTTCCCCGGC TCCAGAATGG CATGGTGCCC AGCCAGTACA ACCCACTACG GCCGGGTGTG 
ACGCCGGGCA CACTGAGCAC ACAGGCAGCT GGCCTCCAGC ATAGCATGAT GGGGCCACTA 
CACAGCAGCC TCTCCACCAA TACCTTGTCC CCGATTATTT ACCAGGGCCT GCCCAACACA 
CGGCTGGCAA CACAGCCTCA CCTGGTGCAG ACCCAGCAGG TGCAGCCACA GAACTTACAG 
CTCCAGCCTC AGAACCTGCA GCCACCATCA CAGCCACACC TCAGTGTGAG CTCGGCAGCC 
AATGGGCACC TGGGCCGGAG CTTCTTGAGT GGGGAGCCCA GTCAGGCAGA TGTACAACCG 
CTGGGCCCCA GCAGTCTGCC TGTGCACACC ATTCTGCCCC AGGAAAGCCA GGCCCTGCCC 
ACATCACTGC CATCCTCCAT GGTCCCACCC ATGACCACTA CCCAGTTCCT GACCCCTCCT 
TCCCAGCACA GTTACTCCTC CTCCCCTGTG GACAACACCC CCAGCCACCA GCTGCAGGTG 
CCAGAGCACC CCTTCCTCAC CCCATCCCCT GAGTCCCCTG ACCAGTGGTC CAGCTCCTCC 
CCGCATTCCA ACATCTCTGA TTGGTCCGAG GGCATCTCCA GCCCGCCCAC CACCATGCCG 
TCCCAGATCA CCCACATTCC AGAGGCATTT AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Primary cilia in murine palatal rugae development.
Gene expression patterns : GEP34119062(2019 12)
Nakaniwa M,Kawasaki M,Kawasaki K,Yamada A,Meguro F,Takeyasu M,Ohazama A

Niche-Specific Factors Dynamically Regulate Sebaceous Gland Stem Cells in the Skin.
Developmental cell51(3)326-340.e4(2019 Nov)
Veniaminova NA,Grachtchouk M,Doane OJ,Peterson JK,Quigley DA,Lull MV,Pyrozhenko DV,Nair RR,Patrick MT,Balmain A,Dlugosz AA,Tsoi LC,Wong SY

A modifier in the 129S2/SvPasCrl genome is responsible for the viability of Notch1[12f/12f] mice.
BMC developmental biology19(1)19(2019 10)
Varshney S,Wei HX,Batista F,Nauman M,Sundaram S,Siminovitch K,Tanwar A,Stanley P

Altered keratinocyte differentiation is an early driver of keratin mutation-based palmoplantar keratoderma.
Human molecular genetics28(13)2255-2270(2019 07)
Zieman AG,Poll BG,Ma J,Coulombe PA

Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes.
Nature communications10(1)2817(2019 06)
Diéguez-Hurtado R,Kato K,Giaimo BD,Nieminen-Kelhä M,Arf H,Ferrante F,Bartkuhn M,Zimmermann T,Bixel MG,Eilken HM,Adams S,Borggrefe T,Vajkoczy P,Adams RH

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Notch1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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