notch1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	notch1b
Entrez Gene ID	794892
Full Name	notch 1b
Synonyms	etID309871.5,wu:fc55e11
General protein information	Preferred Names notch 1b Names neurogenic locus notch homolog protein 1 notch homolog 1b notch receptor protein 1b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005168649.3	XP_005168706.1	neurogenic locus notch homolog protein 1 isoform X1
XM_005168649.4	XP_005168706.1	neurogenic locus notch homolog protein 1 isoform X1
NM_131302.2	NP_571377.2	neurogenic locus notch homolog protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NOTCH1	XP_002691666.2
Gallus gallus (chicken)	NOTCH1	XP_415420.4
Pan troglodytes (chimpanzee)	NOTCH1	XP_520371.2
Macaca mulatta (Rhesus monkey)	LOC709947	XP_002808138.1
Rattus norvegicus (Norway rat)	Notch1	NP_001099191.1
Drosophila melanogaster (fruit fly)	N	NP_001245510.1
Mus musculus (house mouse)	Notch1	NP_032740.3
Canis lupus familiaris (dog)	NOTCH1	XP_005625490.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001015	XP_309208.5
Homo sapiens (human)	NOTCH1	NP_060087.3
Danio rerio (zebrafish)	notch1b	NP_571377.2
Xenopus tropicalis (tropical clawed frog)	notch1	NP_001090757.1

Gene Ontology
Bibliography

Related articles in PubMed

Hemodynamic-mediated endocardial signaling controls in vivo myocardial reprogramming.
Gálvez-Santisteban M, Chen D, Zhang R, Serrano R, Nguyen C, Zhao L, Nerb L, Masutani EM, Vermot J, Burns CG, Burns CE, Del Álamo JC, Chi NC
eLife8(2019 06)

EGFR is required for Wnt9a-Fzd9b signalling specificity in haematopoietic stem cells.
Grainger S, Nguyen N, Richter J, Setayesh J, Lonquich B, Oon CH, Wozniak JM, Barahona R, Kamei CN, Houston J, Carrillo-Terrazas M, Drummond IA, Gonzalez D, Willert K, Traver D
Nature cell biology21(6)721-730(2019 06)

Identification of a 42-bp heart-specific enhancer of the notch1b gene in zebrafish embryos.
Wang F, Yang Q, Wu F, Zhang Y, Sun S, Wang X, Gui Y, Li Q
Developmental dynamics : an official publication of the American Association of Anatomists248(6)426-436(2019 06)

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Xue Y, Liu D, Cui G, Ding Y, Ai D, Gao S, Zhang Y, Suo S, Wang X, Lv P, Zhou C, Li Y, Chen X, Peng G, Jing N, Han JJ, Liu F
Cell reports27(5)1567-1578.e5(2019 04)

Leptin-a mediates transcription of genes that participate in central endocrine and phosphatidylinositol signaling pathways in 72-hour embryonic zebrafish (
Tuttle M, Dalman MR, Liu Q, Londraville RL
PeerJ7e6848(2019)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following notch1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the notch1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03518	NM_131302.2 Latest version!	Danio rerio notch receptor 1b (notch1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa11194	XM_005168649.4 Latest version!	Danio rerio notch 1b (notch1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
ODa11194	XM_005168649.3	Danio rerio notch 1b (notch1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

notch1b ( NM_131302.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571377.2 Danio rerio notch receptor 1b (notch1b), mRNA.

Price & Availability↑

CloneID	ODa03518
Clone ID Related Accession (Same CDS sequence)	NM_131302.2
Accession Version	NM_131302.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576252800000
Organism	Danio rerio(zebrafish)
Product	neurogenic locus notch homolog protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY805407.1. On or before Nov 27, 2007 this sequence version replaced XM_001334426.1, NM_131302.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY805407.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCATCTTT TCTTCGTGAA ACTAATTGTT GTGATATCAC TCAATACTTT GACACAAGGC 
CTGGAGTGTT CAGAAAAATG CCAAAACGGA GGCACATGTG AACCAACTGC AGACGGGAGA 
GGAGAATGCA AGTGTCTGGA TCTGTACGCC GGCCCAGCGT GCCAGTTCCG CAACCCTTGC 
TTCCAATCCC CGTGCAGAAA TGGTGGCGTG TGCCGTTTGA TCACTTCTGC CAACAAGGTG 
GACTTTGTCT GCAACTGCAG CCTGGGCTAC ACGGACCGAC TGTGCCTCAC TCCCACCAAC 
AACGTGTGCC TTGGTGCTCC GTGCCGAAAC GGAGGCACGT GCGAACTCAC CAGCATCCAC 
AACTACAGGT GTAAATGCCC ACCAGGCTGG TCAGGTAAAA CCTGCCAGCA AGCTGATCCC 
TGTGCCTCGA ATCCTTGTGC CAATGGTGGT CAGTGTAGCC CCTTTGATTC GGACTTCCTC 
TGCCACTGTA CACCTTACTT CTCAGGCCAA ACCTGCAAAC AAGATGTCAA CGAGTGCGCG 
CAGATTCCCT CTCCGTGTAA GAACGGTGGC GTGTGTGAGA ACGGAGTGGG CACGTATCAC 
TGCAACTGTC CCGCCGAGTA CACTGGGAAA CACTGCGAGA GCCTGTATCA GCCGTGTAAC 
CCCTCGCCAT GCTTACACGG GGGCACCTGC GTACAGAAGG GGGAGACCAG TTATGAGTGC 
TCCTGTCTGC CAGGTCGGCA GGAATTTGTG CTTATCATGA GGAAATGTCT GAACGACTAC 
TTCCTTATGC TGGTGTTCAT AGGTTTTAGT GGGCAGAACT GCGAAGAAAA CATCGATGAT 
TGTCCAGATC ATCGCTGCCT TAATGGAGGG ACCTGTGTGG ATGGAGTGAA CACCTACAAT 
TGCCAGTGCA AACCAGAGTG GACAGGTCAG TTCTGTACCG AAGATGTCAA CGAGTGCGAT 
TTGATGCCCA ACTCCTGCCA GAACGGTGGC ACGTGCTTGA ACACGCAGGG TGGGTATAAC 
TGTGTGTGCG TGAACGGCTG GACGGGGGAC GACTGCAGCG AGAACATTGA CGACTGCGCA 
GATGCAGCCT GTCATACTGG AGCCACATGC CACGATCGGG TGGCCTCCTT CCTCTGCGAA 
TGTCCTCACG GGCGCACAGG TCTGTTGTGT CATCTAGATG ATGCTTGCAT TAGTAACCCG 
TGTCAAAAAG GCTCTAACTG TGACACCAAC CCAGTCAACG GCAAAGCCAT CTGTACCTGT 
CCACTGGGTT ATGTTGGGCC GGCCTGTGAC CAAGACGTTG ATGAATGCTC ACTGGGTGCA 
AACCCTTGTG AACATGCTGG AAAGTGTATA AACACAAAAG GCTCGTTCCA GTGTAAATGT 
CTGCAGGGAT ATGTGGGAGC TCGATGTGAG CTGGACATAA ATGAGTGCCT TTCCACCCCG 
TGCCAAAACG ACGCCACCTG TTTAGATCAG ATTGGAGGCT TTCATTGCAT CTGTATGCCA 
GGGTACGAGG GAGTCTTCTG TCAGATCAAT ACCGATGAGT GCGCCAGCAT GCCCTGCCTC 
AACAATGGGA AGTGCATCGA CAAGATCAAC AACTACCAAT GCGAGTGCCC CACAGGATTT 
TCGGGGAGCC AATGCCAGTT CGACATTGAC GAGTGCGCTA GTACTCCTTG TAAAAATGGG 
GCCAAGTGTA TGGATGGGCC TAATATGTAC ACCTGCCAGT GCACTGAAGG ATACACAGGG 
CAGCACTGTG AGACAGATGT AGATGAGTGT CTGTCCAACC CGTGTCACTA CGGCACCTGT 
AAAGATGGCC TGGCGTCCTT CACCTGCGTC TGCCGTGCGG GTTTCATGGG CCGTCTGTGC 
GAGATCAACA TTAATGAGTG TCTCAGTCAG CCATGCCAAA ACGGAGGCAC CTGCCAGGAC 
CGTGAGAATG CCTACTTGTG TGTCTGCCCC AAAGGAACTG CAGGAGCTAA CTGTGAGATC 
AACCTGGATG ACTGTCAGAG CAATCCCTGT GATTTCGGGA GATGCATCGA CAAGATAAAT 
GGGTACGAGT GTGCCTGCGA ACCGGGATAC ACAGGGAAAA TGTGCAACGT CAATATCGAT 
GAATGTGCCA TCAACCCCTG CCACAATGGC GGAACCTGTG TGGATGGAGT CAATGGCTTC 
ACTTGTCTGT GCAGAGAGGG CTATCATGAC ACCACCTGCC AATCACAGCT CAACGAATGC 
CTCAGCAACC CCTGTATCCA TGGACACTGT GAAGACAAAG TGAATGGATA TAACTGTATT 
TGCGACTCTG GCTGGAGTGG AGTCAACTGT GACATTAACA ACAACGAGTG CGAGTCCAAC 
CCCTGTATGA ATGGAGGCAC TTGTAAGGAC ATGACCAGTG GCTACGTCTG CACATGTCGC 
GCCGGCTTCA GCGGTCCCAA TTGCCAAACG AATATCAACG AATGTGCCTC CAACCCATGC 
TTGAATCAAG GAACCTGCAT TGATGATGTG GCCGGATACA AATGCAACTG TCTGCTTCCT 
TACACTGGCC AAACATGTGA GGTGGACATC AACGAGTGTG TTAAAAATCC TTGCCGAAAT 
GATGCCATCT GCCAAAACTC CATTGGCAGC TACAAGTGCA GTTGCAAAGC GGGCTACACG 
GGCCGCAACT GTGAGACAGA CATCGATGAC TGCAAGCCCA ACCCCTGCAG TAACGGTGGC 
TTCTGCAAAG ACGCAGTGAA CGCCTTCACT TGCACCTGCC TGCCGGGTTT CAGGGGCGGC 
AGATGCGAGG AGGACATTAA CGAATGTGAG AGTAACCCGT GTAAAAACGG CGCCAACTGC 
ACTGATTGTG TGAACAGCTA CACCTGCACC TGTCCACCTG GATTCAGTGG GATTCACTGC 
GAGAACAACA CACCTGACTG TACTGAGAGC TCTTGTTTTA ATGGTGGCAC ATGTGTGGAT 
GGCATCAACA GCTTCACTTG CCTGTGCCCT AAAGGCTTCA CTGGCAACTA CTGCCAGCAT 
GACATCAACG AGTGCGACTC CAGACCGTGC ATGAATGGAG GCACCTGCCA GGACAGCTAT 
GGCACCTACA AGTGCACCTG CCCTCAGGGA TACCACGGTC TTAACTGTCA GGAGCTGGTG 
AACTGGTGTA AACCGTCTCC CTGTAAGAAT GGAGGGATCT GCAGACAGAG CGGCACAAGA 
TACAGCTGTC AGTGTCAGAC AGGCTGGACT GGTTTATACT GTGACGTTCC CAGTGTTTCC 
TGCGAGGTGG CCGCCAAACA GCAAGGTGTT GATGTGGTCC GGCTGTGTCG TAACTCTGGC 
CAGTGTCTGG ACGCTGGAAA CACACACTAT TGTCACTGTC AGGCCGGATA CACGGGCAGC 
TACTGTGAGG AGCAGGTGGA CGAATGCATT CCCAATCCAT GCCAGAACGG AGCAACTTGC 
ACCGACTACC TGGGCGGATA CTCCTGTGAA TGTGTGCCAG GTTATCATGG AGTGAACTGC 
TCTGATGAGA TCAATGAGTG TCTGTCTCAG CCCTGCCAGA ACGGGGGCAC ATGCATCGAC 
CTGATCAATA CCTACAAATG CTCCTGTCCT CGGGGAACAC AAGGTGTGCA CTGCGAGATC 
AACATTGACG ACTGCACACC GTTCACTGAC CCCATCACCC ATGAGCCTAA ATGCTTTAAC 
CAGGGCCGCT GTGTGGACCG TGTGGGCGGC TACCACTGCA TCTGTCCCCC TGGGTATGTT 
GGGGAACGCT GTGAGGGCGA CGTCAACGAG TGCCTGTCCA ACCCCTGTGG CACACACAGC 
TGCATCCAGC TCAAAAACAA CTACCGCTGC GAGTGTCGCA CAGGATACAC AGGTCAGCAT 
TGTGACAAAG TGTTTGATGG CTGTAAAGGG AAGCCGTGTC GTAATGGAGG CACCTGCGCT 
GTAGCCAGTA ACACTCCTCA TGGCTTTATC TGCAAATGTC CACCGGGTTT TACGGGCTCG 
ACCTGCGAGT ACGACGCACA CGCATGTGGG AGCCTTCAAT GCAAAAATGG CGGGACGTGC 
GTTTCTGGTC ACAAGAGTCC CAAATGCCTG TGCACCCCTG CTTTCACTGG TCCCGAGTGC 
CAGGATCCCA GCGGAGGCCA CTGCACCACT AACCCCTGCT ATAACGGAGG CACGTGCGAG 
TATATAACCG AGGAGCCGTA TTACCACTGC ATCTGTCCCA CCAACTTCAA CGGCCTCTTC 
TGCCACATCC TGGACTGGAG TTTTCCAGGC GGCACTGGTC AGGACATCAC GCCGGCTCCT 
AAGGTGTCGG TCAGCTGCGA GATTGAGCAG TGTAAAGTCA AGAAGGGCAA TAAGATCTGT 
GACAGCGCAT GTAATAATTA CGCCTGCGAT TGGGACGGTG GCGACTGTTC GCTGAACTTT 
AATGACCCAT GGAAGAACTG TTCGGCGGCT CTGCAGTGCT GGCGCTATTT TAATAACGGG 
AAGTGTGATG AACAGTGCCA CAACACGGGA TGCCTCTATG ATGGGTTCGA CTGCCAGAGA 
GTGGAGGCAC AGTGCAATCC ACTATACGAT CAGTATTGCA AGGACCACTT TGCAGACGGC 
TACTGTGACC AGGGCTGCAA TAATGCAGAG TGTGAATGGG ACGGCCTGGA CTGCGCTAAC 
GACACCCCCG AGAAGCTGGC TGCAGGACTG CTGGTTGTGG TGGTCCACAT TCACCCTGAT 
CAGCTCCGAA ACAACTCGTT CGGCTTTCTG CGGGAGCTCA GCCGAGTGCT CCACACCAAC 
GTGGTTTTCC GGCGGGATAG CAAGGGGCAA GAAATGATCT ACCCGTATTA CGGCAATGAG 
CAGGAACTGA AGAAACACAA CATCAAACGA TCACTAGACG GCTGGAACGA TGCTTCTAGC 
GATGTCTTGA GCTCGATGAA AAACAGCATT TACAATATAG TAGTGGAAGG AGGGAGAAAA 
CGCAGAGAAC TGGAGAAGAT ACAAGTCAAA GGCTCCGTGG TTTATCTGGA GATCGACAAC 
CGGCAGTGCT ACCAGCAAAC CTCCGAATGC TTCCAGAGCG CCAACGACGC GGCGGCATTC 
CTCGGAGCGC TGGCTTCCAG CGGCAGTCTG AAAATGCCTT ACGTCATCGA GGCCGTCACA 
AGTGAGATCG ATGGACCGTC GCCTGTAGAG CTCTATCCAG TCTACGTGGT TCTGGCAGGA 
TTGGCGCTGC TGGCCTTTGT CGCCATTGGG ATGGTGGCGT CCCGTAAACG GCGCCGGGAA 
CACGGACAAC TTTGGTTTCC GGAGGGTTTT AAGACCAGCG AGCCCAGCAA GAAGAAGAGG 
AGAGAGCCGG TCGGAGAAGA TTCAGTCGGT TTGAGGCCAC TCAAGAACTG TTCAGACATT 
TCGTTAATGG ACGACAACCA GAATGAATGG GGAGAGGAGG AACAGTCCGA CAGCAAACGC 
TTCAGGTCTG AGGAGCAGGC AATGCTGGAT TTAGACGATC AGCCAGATCA CAGACAGTGG 
ACGCAGCAGC ATTTGGACGC AGCCGATCTG CGCATCCCAT CCATCGCTCC CACACCGCCT 
CAGGGCGAGA TAGAGAACGA CTGCATGGAC GTCAATGCCC GAGGACCAGA TGGCTTTACT 
CCTCTGATGA TTGCATCCTG CAGTGGAGGA GGGCTCGAAA CGGGAAATAG CGAGGAAGAG 
GAAGACGCTT CAGCAAACGT CATCAATGAC TTCATCTATC AGGGCGCCAA CCTTCATAAC 
CAGACAGACC GCACGGGTGA GACGGCTCTA CACCTGGCTG CCCGCTACGC CCGCTCAGAC 
GCCGCGAAAC GACTGCTGGA GGCCAGTGCT GATGCTAACA TCCAGGACAA TATGGGCAGG 
ACTCCATTAC ATGCCGCTGT GGCTGCTGAT GCCCAAGGCG TCTTCCAGAT TTTGATACGG 
AACCGTGCCA CAGATCTAGA CGCCCGCATG CATGATGGCA CCACACCTCT GATACTGGCC 
GCAAGACTGG CGGTCGAGGG CATGGTGGAG GAACTCATCA ACTGCCATGC TGACGTTAAT 
GCCATTGATG ACTTTGGTAA ATCTGCTCTT CACTGGGCAG CAGCTGTGAA CAATGTAGAT 
GCAGCGATGG TGTTGCTTAA GAATGGGGCA AATAAAGACA TGCAGAACAA TAAGGAGGAG 
ACTCCTCTTT TCCTGGCGGC AAGGGAAGGA AGTTACGAGA CGGCTAAAGT TCTTCTGGAG 
CATTTTGCAA ACCGGGAAAT CACAGACCAC ATGGATCGAC TCCCGCGAGA CATCGCGCAA 
GACAGAATGC ATCATGATAT TGTGCGTCTT ATTGATGAAT ACAACCTAGT GCGCAGTCCT 
CCCATGCACA GCGCCCCGCT CTGCACCACC CTTTCCCCAC CCCTCTGCTC CCCCAATGGC 
TTCATGGGCA ACATGAAGCC CTCGGTGCAA AGCAAAAAAC CTCGTAAGCC CAGCACTAAA 
GGCATCGGCT GCAAAGACGG CAAAGATATG AAAGTCAAAA AGAAGAAAGC GCAAGACGGA 
AAGGGAAACT TATTAGACAG CTCGGCTGTT CTCTCTCCGG TGGATTCCCT AGAGTCGCCT 
CACGGGTATA TTTCTGACAT CGCCTCGCCA CCCCAGATGA CATCACCCTT CCAACAATCG 
CCCTCCATGT CGCTCAATCA GCTACAGGGA ATGTCAGACA ACCACATGGG CGTCAGCCAT 
CTTGGAATTG GCAACAACCA AGATCTTTCC CATATACAGT TTGATCCATT GCCTCCACGT 
CTCACTCATC TTCCGGTGGC CGGATCGAAC GGTTCAAACG TCATGAACGG TCAATGCGAA 
TGGCTAGAGC GGATGCATGG AAGCATGGCT CCGCAAAACC AGTTCACAGC CATGAGAAAC 
GCCTCAGGTC AAGCCAACCT TCACCAATCG GGCTTGATGA CGTCACATCA CAATGGCCGC 
CCTGCCACGC TCTCTCAAAT GATGAACTAT CAAAGCATGC AGAACACACA CCTAATGCAG 
CAGATGCAGC AGAGCATGCA GCCGCGGCCC CAGCAGACCG GCGTCCAGCT GCAGAGCCAG 
AGCCAGAACT TCATCGGGGG TGATCTGGGT GGACCGGAGC TCCAGCAGAG CGCAGGGAAC 
AGCATGTCCA TCCATACTAT AATCCCCCAG GAGACCCAGC TGCTCAACCC GTCCTCTCTG 
GGGTCCAGCA TGGCGGGCAC GCAGTTCCTG ACGCCACCTT CCCAGCACAG TTACACTCCC 
GCATTGGACG CCAATACTCC CAACCACCAA CTTCAAGTGC CTGACCACCA TCCTTTCCTC 
ACGCCATCTC CCGGTTCTCC AGACCAGTGG TCCAGCTCGT CACCCAATTC CAACATGTCC 
GATTGGTCGG AGGGTATATC CAGTCCTCCC ACCAGCATGC AGTCACAAAT CGGACACATG 
CCCGAGCAAT TCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571377.2
CDS	142..7539
Translation	MHLFFVKLIVVISLNTLTQGLECSEKCQNGGTCEPTADGRGECKCLDLYAGPACQFRNPCFQSPCRNGGVCRLITSANKVDFVCNCSLGYTDRLCLTPTNNVCLGAPCRNGGTCELTSIHNYRCKCPPGWSGKTCQQADPCASNPCANGGQCSPFDSDFLCHCTPYFSGQTCKQDVNECAQIPSPCKNGGVCENGVGTYHCNCPAEYTGKHCESLYQPCNPSPCLHGGTCVQKGETSYECSCLPGRQEFVLIMRKCLNDYFLMLVFIGFSGQNCEENIDDCPDHRCLNGGTCVDGVNTYNCQCKPEWTGQFCTEDVNECDLMPNSCQNGGTCLNTQGGYNCVCVNGWTGDDCSENIDDCADAACHTGATCHDRVASFLCECPHGRTGLLCHLDDACISNPCQKGSNCDTNPVNGKAICTCPLGYVGPACDQDVDECSLGANPCEHAGKCINTKGSFQCKCLQGYVGARCELDINECLSTPCQNDATCLDQIGGFHCICMPGYEGVFCQINTDECASMPCLNNGKCIDKINNYQCECPTGFSGSQCQFDIDECASTPCKNGAKCMDGPNMYTCQCTEGYTGQHCETDVDECLSNPCHYGTCKDGLASFTCVCRAGFMGRLCEININECLSQPCQNGGTCQDRENAYLCVCPKGTAGANCEINLDDCQSNPCDFGRCIDKINGYECACEPGYTGKMCNVNIDECAINPCHNGGTCVDGVNGFTCLCREGYHDTTCQSQLNECLSNPCIHGHCEDKVNGYNCICDSGWSGVNCDINNNECESNPCMNGGTCKDMTSGYVCTCRAGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGQTCEVDINECVKNPCRNDAICQNSIGSYKCSCKAGYTGRNCETDIDDCKPNPCSNGGFCKDAVNAFTCTCLPGFRGGRCEEDINECESNPCKNGANCTDCVNSYTCTCPPGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPKGFTGNYCQHDINECDSRPCMNGGTCQDSYGTYKCTCPQGYHGLNCQELVNWCKPSPCKNGGICRQSGTRYSCQCQTGWTGLYCDVPSVSCEVAAKQQGVDVVRLCRNSGQCLDAGNTHYCHCQAGYTGSYCEEQVDECIPNPCQNGATCTDYLGGYSCECVPGYHGVNCSDEINECLSQPCQNGGTCIDLINTYKCSCPRGTQGVHCEINIDDCTPFTDPITHEPKCFNQGRCVDRVGGYHCICPPGYVGERCEGDVNECLSNPCGTHSCIQLKNNYRCECRTGYTGQHCDKVFDGCKGKPCRNGGTCAVASNTPHGFICKCPPGFTGSTCEYDAHACGSLQCKNGGTCVSGHKSPKCLCTPAFTGPECQDPSGGHCTTNPCYNGGTCEYITEEPYYHCICPTNFNGLFCHILDWSFPGGTGQDITPAPKVSVSCEIEQCKVKKGNKICDSACNNYACDWDGGDCSLNFNDPWKNCSAALQCWRYFNNGKCDEQCHNTGCLYDGFDCQRVEAQCNPLYDQYCKDHFADGYCDQGCNNAECEWDGLDCANDTPEKLAAGLLVVVVHIHPDQLRNNSFGFLRELSRVLHTNVVFRRDSKGQEMIYPYYGNEQELKKHNIKRSLDGWNDASSDVLSSMKNSIYNIVVEGGRKRRELEKIQVKGSVVYLEIDNRQCYQQTSECFQSANDAAAFLGALASSGSLKMPYVIEAVTSEIDGPSPVELYPVYVVLAGLALLAFVAIGMVASRKRRREHGQLWFPEGFKTSEPSKKKRREPVGEDSVGLRPLKNCSDISLMDDNQNEWGEEEQSDSKRFRSEEQAMLDLDDQPDHRQWTQQHLDAADLRIPSIAPTPPQGEIENDCMDVNARGPDGFTPLMIASCSGGGLETGNSEEEEDASANVINDFIYQGANLHNQTDRTGETALHLAARYARSDAAKRLLEASADANIQDNMGRTPLHAAVAADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMVEELINCHADVNAIDDFGKSALHWAAAVNNVDAAMVLLKNGANKDMQNNKEETPLFLAAREGSYETAKVLLEHFANREITDHMDRLPRDIAQDRMHHDIVRLIDEYNLVRSPPMHSAPLCTTLSPPLCSPNGFMGNMKPSVQSKKPRKPSTKGIGCKDGKDMKVKKKKAQDGKGNLLDSSAVLSPVDSLESPHGYISDIASPPQMTSPFQQSPSMSLNQLQGMSDNHMGVSHLGIGNNQDLSHIQFDPLPPRLTHLPVAGSNGSNVMNGQCEWLERMHGSMAPQNQFTAMRNASGQANLHQSGLMTSHHNGRPATLSQMMNYQSMQNTHLMQQMQQSMQPRPQQTGVQLQSQSQNFIGGDLGGPELQQSAGNSMSIHTIIPQETQLLNPSSLGSSMAGTQFLTPPSQHSYTPALDANTPNHQLQVPDHHPFLTPSPGSPDQWSSSSPNSNMSDWSEGISSPPTSMQSQIGHMPEQFK

Target ORF information:

RefSeq Version	NM_131302.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio notch receptor 1b (notch1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131302.2

ORF Insert Sequence:

ATGCATCTTT TCTTCGTGAA ACTAATTGTT GTGATATCAC TCAATACTTT GACACAAGGC 
CTGGAGTGTT CAGAAAAATG CCAAAACGGA GGCACATGTG AACCAACTGC AGACGGGAGA 
GGAGAATGCA AGTGTCTGGA TCTGTACGCC GGCCCAGCGT GCCAGTTCCG CAACCCTTGC 
TTCCAATCCC CGTGCAGAAA TGGTGGCGTG TGCCGTTTGA TCACTTCTGC CAACAAGGTG 
GACTTTGTCT GCAACTGCAG CCTGGGCTAC ACGGACCGAC TGTGCCTCAC TCCCACCAAC 
AACGTGTGCC TTGGTGCTCC GTGCCGAAAC GGAGGCACGT GCGAACTCAC CAGCATCCAC 
AACTACAGGT GTAAATGCCC ACCAGGCTGG TCAGGTAAAA CCTGCCAGCA AGCTGATCCC 
TGTGCCTCGA ATCCTTGTGC CAATGGTGGT CAGTGTAGCC CCTTTGATTC GGACTTCCTC 
TGCCACTGTA CACCTTACTT CTCAGGCCAA ACCTGCAAAC AAGATGTCAA CGAGTGCGCG 
CAGATTCCCT CTCCGTGTAA GAACGGTGGC GTGTGTGAGA ACGGAGTGGG CACGTATCAC 
TGCAACTGTC CCGCCGAGTA CACTGGGAAA CACTGCGAGA GCCTGTATCA GCCGTGTAAC 
CCCTCGCCAT GCTTACACGG GGGCACCTGC GTACAGAAGG GGGAGACCAG TTATGAGTGC 
TCCTGTCTGC CAGGTCGGCA GGAATTTGTG CTTATCATGA GGAAATGTCT GAACGACTAC 
TTCCTTATGC TGGTGTTCAT AGGTTTTAGT GGGCAGAACT GCGAAGAAAA CATCGATGAT 
TGTCCAGATC ATCGCTGCCT TAATGGAGGG ACCTGTGTGG ATGGAGTGAA CACCTACAAT 
TGCCAGTGCA AACCAGAGTG GACAGGTCAG TTCTGTACCG AAGATGTCAA CGAGTGCGAT 
TTGATGCCCA ACTCCTGCCA GAACGGTGGC ACGTGCTTGA ACACGCAGGG TGGGTATAAC 
TGTGTGTGCG TGAACGGCTG GACGGGGGAC GACTGCAGCG AGAACATTGA CGACTGCGCA 
GATGCAGCCT GTCATACTGG AGCCACATGC CACGATCGGG TGGCCTCCTT CCTCTGCGAA 
TGTCCTCACG GGCGCACAGG TCTGTTGTGT CATCTAGATG ATGCTTGCAT TAGTAACCCG 
TGTCAAAAAG GCTCTAACTG TGACACCAAC CCAGTCAACG GCAAAGCCAT CTGTACCTGT 
CCACTGGGTT ATGTTGGGCC GGCCTGTGAC CAAGACGTTG ATGAATGCTC ACTGGGTGCA 
AACCCTTGTG AACATGCTGG AAAGTGTATA AACACAAAAG GCTCGTTCCA GTGTAAATGT 
CTGCAGGGAT ATGTGGGAGC TCGATGTGAG CTGGACATAA ATGAGTGCCT TTCCACCCCG 
TGCCAAAACG ACGCCACCTG TTTAGATCAG ATTGGAGGCT TTCATTGCAT CTGTATGCCA 
GGGTACGAGG GAGTCTTCTG TCAGATCAAT ACCGATGAGT GCGCCAGCAT GCCCTGCCTC 
AACAATGGGA AGTGCATCGA CAAGATCAAC AACTACCAAT GCGAGTGCCC CACAGGATTT 
TCGGGGAGCC AATGCCAGTT CGACATTGAC GAGTGCGCTA GTACTCCTTG TAAAAATGGG 
GCCAAGTGTA TGGATGGGCC TAATATGTAC ACCTGCCAGT GCACTGAAGG ATACACAGGG 
CAGCACTGTG AGACAGATGT AGATGAGTGT CTGTCCAACC CGTGTCACTA CGGCACCTGT 
AAAGATGGCC TGGCGTCCTT CACCTGCGTC TGCCGTGCGG GTTTCATGGG CCGTCTGTGC 
GAGATCAACA TTAATGAGTG TCTCAGTCAG CCATGCCAAA ACGGAGGCAC CTGCCAGGAC 
CGTGAGAATG CCTACTTGTG TGTCTGCCCC AAAGGAACTG CAGGAGCTAA CTGTGAGATC 
AACCTGGATG ACTGTCAGAG CAATCCCTGT GATTTCGGGA GATGCATCGA CAAGATAAAT 
GGGTACGAGT GTGCCTGCGA ACCGGGATAC ACAGGGAAAA TGTGCAACGT CAATATCGAT 
GAATGTGCCA TCAACCCCTG CCACAATGGC GGAACCTGTG TGGATGGAGT CAATGGCTTC 
ACTTGTCTGT GCAGAGAGGG CTATCATGAC ACCACCTGCC AATCACAGCT CAACGAATGC 
CTCAGCAACC CCTGTATCCA TGGACACTGT GAAGACAAAG TGAATGGATA TAACTGTATT 
TGCGACTCTG GCTGGAGTGG AGTCAACTGT GACATTAACA ACAACGAGTG CGAGTCCAAC 
CCCTGTATGA ATGGAGGCAC TTGTAAGGAC ATGACCAGTG GCTACGTCTG CACATGTCGC 
GCCGGCTTCA GCGGTCCCAA TTGCCAAACG AATATCAACG AATGTGCCTC CAACCCATGC 
TTGAATCAAG GAACCTGCAT TGATGATGTG GCCGGATACA AATGCAACTG TCTGCTTCCT 
TACACTGGCC AAACATGTGA GGTGGACATC AACGAGTGTG TTAAAAATCC TTGCCGAAAT 
GATGCCATCT GCCAAAACTC CATTGGCAGC TACAAGTGCA GTTGCAAAGC GGGCTACACG 
GGCCGCAACT GTGAGACAGA CATCGATGAC TGCAAGCCCA ACCCCTGCAG TAACGGTGGC 
TTCTGCAAAG ACGCAGTGAA CGCCTTCACT TGCACCTGCC TGCCGGGTTT CAGGGGCGGC 
AGATGCGAGG AGGACATTAA CGAATGTGAG AGTAACCCGT GTAAAAACGG CGCCAACTGC 
ACTGATTGTG TGAACAGCTA CACCTGCACC TGTCCACCTG GATTCAGTGG GATTCACTGC 
GAGAACAACA CACCTGACTG TACTGAGAGC TCTTGTTTTA ATGGTGGCAC ATGTGTGGAT 
GGCATCAACA GCTTCACTTG CCTGTGCCCT AAAGGCTTCA CTGGCAACTA CTGCCAGCAT 
GACATCAACG AGTGCGACTC CAGACCGTGC ATGAATGGAG GCACCTGCCA GGACAGCTAT 
GGCACCTACA AGTGCACCTG CCCTCAGGGA TACCACGGTC TTAACTGTCA GGAGCTGGTG 
AACTGGTGTA AACCGTCTCC CTGTAAGAAT GGAGGGATCT GCAGACAGAG CGGCACAAGA 
TACAGCTGTC AGTGTCAGAC AGGCTGGACT GGTTTATACT GTGACGTTCC CAGTGTTTCC 
TGCGAGGTGG CCGCCAAACA GCAAGGTGTT GATGTGGTCC GGCTGTGTCG TAACTCTGGC 
CAGTGTCTGG ACGCTGGAAA CACACACTAT TGTCACTGTC AGGCCGGATA CACGGGCAGC 
TACTGTGAGG AGCAGGTGGA CGAATGCATT CCCAATCCAT GCCAGAACGG AGCAACTTGC 
ACCGACTACC TGGGCGGATA CTCCTGTGAA TGTGTGCCAG GTTATCATGG AGTGAACTGC 
TCTGATGAGA TCAATGAGTG TCTGTCTCAG CCCTGCCAGA ACGGGGGCAC ATGCATCGAC 
CTGATCAATA CCTACAAATG CTCCTGTCCT CGGGGAACAC AAGGTGTGCA CTGCGAGATC 
AACATTGACG ACTGCACACC GTTCACTGAC CCCATCACCC ATGAGCCTAA ATGCTTTAAC 
CAGGGCCGCT GTGTGGACCG TGTGGGCGGC TACCACTGCA TCTGTCCCCC TGGGTATGTT 
GGGGAACGCT GTGAGGGCGA CGTCAACGAG TGCCTGTCCA ACCCCTGTGG CACACACAGC 
TGCATCCAGC TCAAAAACAA CTACCGCTGC GAGTGTCGCA CAGGATACAC AGGTCAGCAT 
TGTGACAAAG TGTTTGATGG CTGTAAAGGG AAGCCGTGTC GTAATGGAGG CACCTGCGCT 
GTAGCCAGTA ACACTCCTCA TGGCTTTATC TGCAAATGTC CACCGGGTTT TACGGGCTCG 
ACCTGCGAGT ACGACGCACA CGCATGTGGG AGCCTTCAAT GCAAAAATGG CGGGACGTGC 
GTTTCTGGTC ACAAGAGTCC CAAATGCCTG TGCACCCCTG CTTTCACTGG TCCCGAGTGC 
CAGGATCCCA GCGGAGGCCA CTGCACCACT AACCCCTGCT ATAACGGAGG CACGTGCGAG 
TATATAACCG AGGAGCCGTA TTACCACTGC ATCTGTCCCA CCAACTTCAA CGGCCTCTTC 
TGCCACATCC TGGACTGGAG TTTTCCAGGC GGCACTGGTC AGGACATCAC GCCGGCTCCT 
AAGGTGTCGG TCAGCTGCGA GATTGAGCAG TGTAAAGTCA AGAAGGGCAA TAAGATCTGT 
GACAGCGCAT GTAATAATTA CGCCTGCGAT TGGGACGGTG GCGACTGTTC GCTGAACTTT 
AATGACCCAT GGAAGAACTG TTCGGCGGCT CTGCAGTGCT GGCGCTATTT TAATAACGGG 
AAGTGTGATG AACAGTGCCA CAACACGGGA TGCCTCTATG ATGGGTTCGA CTGCCAGAGA 
GTGGAGGCAC AGTGCAATCC ACTATACGAT CAGTATTGCA AGGACCACTT TGCAGACGGC 
TACTGTGACC AGGGCTGCAA TAATGCAGAG TGTGAATGGG ACGGCCTGGA CTGCGCTAAC 
GACACCCCCG AGAAGCTGGC TGCAGGACTG CTGGTTGTGG TGGTCCACAT TCACCCTGAT 
CAGCTCCGAA ACAACTCGTT CGGCTTTCTG CGGGAGCTCA GCCGAGTGCT CCACACCAAC 
GTGGTTTTCC GGCGGGATAG CAAGGGGCAA GAAATGATCT ACCCGTATTA CGGCAATGAG 
CAGGAACTGA AGAAACACAA CATCAAACGA TCACTAGACG GCTGGAACGA TGCTTCTAGC 
GATGTCTTGA GCTCGATGAA AAACAGCATT TACAATATAG TAGTGGAAGG AGGGAGAAAA 
CGCAGAGAAC TGGAGAAGAT ACAAGTCAAA GGCTCCGTGG TTTATCTGGA GATCGACAAC 
CGGCAGTGCT ACCAGCAAAC CTCCGAATGC TTCCAGAGCG CCAACGACGC GGCGGCATTC 
CTCGGAGCGC TGGCTTCCAG CGGCAGTCTG AAAATGCCTT ACGTCATCGA GGCCGTCACA 
AGTGAGATCG ATGGACCGTC GCCTGTAGAG CTCTATCCAG TCTACGTGGT TCTGGCAGGA 
TTGGCGCTGC TGGCCTTTGT CGCCATTGGG ATGGTGGCGT CCCGTAAACG GCGCCGGGAA 
CACGGACAAC TTTGGTTTCC GGAGGGTTTT AAGACCAGCG AGCCCAGCAA GAAGAAGAGG 
AGAGAGCCGG TCGGAGAAGA TTCAGTCGGT TTGAGGCCAC TCAAGAACTG TTCAGACATT 
TCGTTAATGG ACGACAACCA GAATGAATGG GGAGAGGAGG AACAGTCCGA CAGCAAACGC 
TTCAGGTCTG AGGAGCAGGC AATGCTGGAT TTAGACGATC AGCCAGATCA CAGACAGTGG 
ACGCAGCAGC ATTTGGACGC AGCCGATCTG CGCATCCCAT CCATCGCTCC CACACCGCCT 
CAGGGCGAGA TAGAGAACGA CTGCATGGAC GTCAATGCCC GAGGACCAGA TGGCTTTACT 
CCTCTGATGA TTGCATCCTG CAGTGGAGGA GGGCTCGAAA CGGGAAATAG CGAGGAAGAG 
GAAGACGCTT CAGCAAACGT CATCAATGAC TTCATCTATC AGGGCGCCAA CCTTCATAAC 
CAGACAGACC GCACGGGTGA GACGGCTCTA CACCTGGCTG CCCGCTACGC CCGCTCAGAC 
GCCGCGAAAC GACTGCTGGA GGCCAGTGCT GATGCTAACA TCCAGGACAA TATGGGCAGG 
ACTCCATTAC ATGCCGCTGT GGCTGCTGAT GCCCAAGGCG TCTTCCAGAT TTTGATACGG 
AACCGTGCCA CAGATCTAGA CGCCCGCATG CATGATGGCA CCACACCTCT GATACTGGCC 
GCAAGACTGG CGGTCGAGGG CATGGTGGAG GAACTCATCA ACTGCCATGC TGACGTTAAT 
GCCATTGATG ACTTTGGTAA ATCTGCTCTT CACTGGGCAG CAGCTGTGAA CAATGTAGAT 
GCAGCGATGG TGTTGCTTAA GAATGGGGCA AATAAAGACA TGCAGAACAA TAAGGAGGAG 
ACTCCTCTTT TCCTGGCGGC AAGGGAAGGA AGTTACGAGA CGGCTAAAGT TCTTCTGGAG 
CATTTTGCAA ACCGGGAAAT CACAGACCAC ATGGATCGAC TCCCGCGAGA CATCGCGCAA 
GACAGAATGC ATCATGATAT TGTGCGTCTT ATTGATGAAT ACAACCTAGT GCGCAGTCCT 
CCCATGCACA GCGCCCCGCT CTGCACCACC CTTTCCCCAC CCCTCTGCTC CCCCAATGGC 
TTCATGGGCA ACATGAAGCC CTCGGTGCAA AGCAAAAAAC CTCGTAAGCC CAGCACTAAA 
GGCATCGGCT GCAAAGACGG CAAAGATATG AAAGTCAAAA AGAAGAAAGC GCAAGACGGA 
AAGGGAAACT TATTAGACAG CTCGGCTGTT CTCTCTCCGG TGGATTCCCT AGAGTCGCCT 
CACGGGTATA TTTCTGACAT CGCCTCGCCA CCCCAGATGA CATCACCCTT CCAACAATCG 
CCCTCCATGT CGCTCAATCA GCTACAGGGA ATGTCAGACA ACCACATGGG CGTCAGCCAT 
CTTGGAATTG GCAACAACCA AGATCTTTCC CATATACAGT TTGATCCATT GCCTCCACGT 
CTCACTCATC TTCCGGTGGC CGGATCGAAC GGTTCAAACG TCATGAACGG TCAATGCGAA 
TGGCTAGAGC GGATGCATGG AAGCATGGCT CCGCAAAACC AGTTCACAGC CATGAGAAAC 
GCCTCAGGTC AAGCCAACCT TCACCAATCG GGCTTGATGA CGTCACATCA CAATGGCCGC 
CCTGCCACGC TCTCTCAAAT GATGAACTAT CAAAGCATGC AGAACACACA CCTAATGCAG 
CAGATGCAGC AGAGCATGCA GCCGCGGCCC CAGCAGACCG GCGTCCAGCT GCAGAGCCAG 
AGCCAGAACT TCATCGGGGG TGATCTGGGT GGACCGGAGC TCCAGCAGAG CGCAGGGAAC 
AGCATGTCCA TCCATACTAT AATCCCCCAG GAGACCCAGC TGCTCAACCC GTCCTCTCTG 
GGGTCCAGCA TGGCGGGCAC GCAGTTCCTG ACGCCACCTT CCCAGCACAG TTACACTCCC 
GCATTGGACG CCAATACTCC CAACCACCAA CTTCAAGTGC CTGACCACCA TCCTTTCCTC 
ACGCCATCTC CCGGTTCTCC AGACCAGTGG TCCAGCTCGT CACCCAATTC CAACATGTCC 
GATTGGTCGG AGGGTATATC CAGTCCTCCC ACCAGCATGC AGTCACAAAT CGGACACATG 
CCCGAGCAAT TCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

notch1b ( XM_005168649.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168706.1 Danio rerio notch 1b (notch1b), transcript variant X1, mRNA.

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CloneID	ODa11194
Clone ID Related Accession (Same CDS sequence)	XM_005168649.3 , XM_005168649.4
Accession Version	XM_005168649.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	neurogenic locus notch homolog protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCTTT TCTTCGTGAA ACTAATTGTT GTGATATCAC TCAATACTTT GACACAAGGC 
CTGGAATGTT CAGAAAAATG CCAAAACGGA GGCACATGTG AACCAACTGC AGACGGGAGA 
GGAGAATGCA AGTGTCTGGA TCTGTACGCC GGCCCAGCGT GCCAGTTCCG CAACCCTTGC 
TTCCAATCCC CGTGCAGAAA TGGTGGCGTG TGCCGTTTGA TCACTTCTGC CAACAAGGTG 
GACTTTGTCT GCAACTGCAG CCTGGGCTAC ACGGACCGAT TGTGCCTCAC TCCCACCAAC 
AACGTGTGCC TTGGTGCTCC GTGCCGAAAC GGAGGAACGT GCGAACTCAC CAGCATCCAC 
AACTACAGGT GTAAATGCCC ACCAGGCTGG TCAGGTAAAA CCTGCCAGCA AGCTGATCCC 
TGTGCCTCGA ATCCTTGTGC CAATGGTGGT CAGTGTAGCC CCTTTGATTC GGACTTCCTC 
TGCCACTGTA CACCTTACTT CTCAGGCCAA ACCTGCAAAC AAGATGTCAA CGAGTGCGCG 
CAGATTCCCT CTCCGTGTAA GAACGGTGGC GTGTGTGAGA ACGGAGTGGG CACGTATCAC 
TGCAACTGTC CCGCCGAGTA CACTGGGAAA CACTGCGAGA GCCTGTATCA GCCGTGTAAC 
CCCTCGCCAT GCTTACACGG GGGCACCTGC GTACAGAAGG GGGAGACCAG TTATGAGTGC 
TCCTGTCTGC CAGGTTTTAG TGGGCAGAAC TGCGAAGAAA ACATCGATGA TTGTCCAGAT 
CATCGCTGCC TTAATGGAGG GACCTGTGTG GATGGAGTGA ACACCTACAA TTGCCAGTGC 
AAACCAGAGT GGACAGGTCA GTTCTGTACC GAAGATGTCA ACGAGTGCGA TTTGATGCCC 
AACTCCTGCC AGAACGGTGG CACGTGCTTG AACACGCAGG GTGGGTATAA CTGTGTGTGC 
GTGAACGGCT GGACGGGGGA CGACTGCAGC GAGAACATCG ACGACTGCGC AGATGCAGCC 
TGTCATACTG GAGCCACATG CCACGATCGG GTGGCCTCCT TCCTCTGCGA ATGTCCTCAC 
GGGCGCACAG GTCTGTTGTG TCATCTAGAT GATGCTTGCA TTAGTAACCC GTGTCAAAAA 
GGCTCTAACT GTGACACCAA CCCAGTCAAC GGCAAAGCCA TCTGTACCTG TCCACTGGGT 
TATGTTGGGC CGGCCTGTGA CCAAGACGTT GATGAATGCT CACTGGGTGC AAACCCTTGT 
GAACATGCTG GAAAGTGTAT AAACACAAAA GGCTCGTTCC AGTGTAAATG TCTGCAGGGA 
TATGTTGGAG CTCGATGTGA GCTGGACATA AATGAGTGCC TTTCCACCCC GTGCCAAAAC 
GACGCCACCT GTTTAGATCA GATTGGAGGA TTCCATTGCA TCTGTATGCC AGGGTACGAG 
GGAGTCTTCT GTCAGATCAA TACCGATGAG TGCGCCAGCA TGCCCTGCCT CAACAATGGG 
AAGTGCATCG ACAAGATCAA CAACTACCAA TGCGAGTGCC CCACAGGATT TTCGGGGAGC 
CAATGCCAGT TCGACATTGA CGAGTGCGCT AGTACTCCTT GTAAAAATGG GGCCAAGTGT 
ATGGATGGGC CCAATATGTA CACCTGCCAG TGCACTGAAG GATACACAGG GCAGCACTGT 
GAGACAGATG TAGATGAGTG TCTGTCCAAC CCGTGTCACT ACGGCACCTG TAAAGATGGC 
CTGGCGTCCT TCACCTGCGT CTGCCGTGCG GGTTTCATGG GCCGTCTGTG CGAGATCAAC 
ATTAATGAGT GTCTCAGTCA GCCATGCCAA AACGGAGGCA CCTGCCAGGA CCGTGAGAAT 
GCCTACTTGT GTGTCTGCCC CAAAGGAACT GCAGGAGCTA ACTGTGAGAT CAACCTGGAT 
GACTGTCAGA GCAATCCCTG TGATTTCGGG AGATGCATCG ACAAGATAAA TGGGTACGAG 
TGTGCCTGCG AACCGGGATA CACAGGGAAA ATGTGCAACA TCAATATCGA TGAATGTGCC 
ATCAACCCCT GCCACAATGG CGGAACCTGT GTGGATGGAG TCAATGGCTT CACTTGTCTG 
TGCAGAGAGG GCTATCATGA CACCACCTGC CAATCACAGC TCAACGAATG CCTCAGCAAC 
CCCTGTATCC ATGGACACTG TGAAGACAAA GTCAATGGAT ATAACTGTAT TTGCGACTCT 
GGCTGGAGTG GAGTCAACTG TGACATTAAC AACAACGAGT GCGAGTCCAA CCCCTGTATG 
AATGGAGGCA CTTGTAAGGA CATGACCAGT GGCTACGTCT GCACATGTCG CGCCGGCTTC 
AGCGGTCCCA ATTGCCAAAC GAATATCAAC GAATGTGCCT CCAACCCATG CTTGAATCAA 
GGAACCTGCA TTGATGATGT GGCCGGATAC AAATGCAACT GTCTGCTTCC TTACACTGGC 
GAGAACTGTG AGACCCTGTT GGCCCCGTGC GGCTCTAAAC CCTGTAAGAA CGGAGGCGTT 
TGCAAGGAAT CGGAAGACTA CGAGAGCTTC TCTTGTGTGT GTCCTGCGGG ATGGCAAGGC 
CAAACATGTG AGGTGGACAT CAACGAGTGT GTTAAAAATC CTTGCCGAAA TGACGCCATC 
TGCCAAAACT CCATTGGCAG CTACAAGTGC AGTTGCAAAG CGGGCTACAC GGGTCGCAAC 
TGTGAGACAG ACATCGATGA CTGCAAGCCC AACCCCTGCA GTAACGGTGG CTTCTGCAAA 
GACGCAGTGA ACGCCTTCGC TTGCACCTGC CTGCCGGGTT TCAGGGGCGG CAGATGCGAG 
GAGGACATTA ACGAATGTGA GAGTAACCCG TGTAAAAACG GCGCCAACTG CACTGATTGT 
GTGAACAGCT ACACCTGCAC CTGTCCACCT GGATTCAGTG GGATTCACTG CGAGAACAAC 
ACACCTGACT GTACTGAGAG CTCTTGTTTT AATGGTGGCA CATGTGTGGA TGGCATCAAC 
AGCTTCACTT GCCTGTGCCC TAAAGGCTTC ACTGGCAACT ACTGCCAGCA TGACATCAAC 
GAGTGCGACT CCAGACCGTG CATGAATGGA GGCACCTGCC AGGACAGCTA TGGCACCTAC 
AAGTGCACCT GCCCTCAGGG ATACCACGGT CTTAACTGTC AGGAGCTGGT GAACTGGTGT 
AAACCGTCTC CCTGTAAGAA TGGAGGGATC TGCAGACAGA GCGGCACGAG ATACAGCTGT 
CAGTGTCAGA CAGGCTGGAC TGGTTTATAC TGTGACGTTC CCAGTGTTTC CTGCGAGGTG 
GCCGCCAAAC AGCAAGGTGT TGATGTGGTC CGGCTGTGTC GTAACTCTGG CCAGTGTCTG 
GACGCTGGAA ACACACACTA TTGTCACTGT CAGGCCGGAT ACACGGGCAG CTACTGTGAG 
GAGCAGGTGG ACGAATGCAT TCCCAATCCG TGCCAGAACG GAGCAACTTG CACCGACTAC 
CTGGGCGGAT ACTCCTGTGA ATGTGTGCCA GGTTATCATG GAGTGAACTG CTCTGATGAG 
ATCAATGAGT GTCTGTCTCA GCCCTGCCAG AACGGGGGCA CATGCATCGA CCTGATCAAT 
ACCTACAAAT GCTCCTGTCC TCGGGGAACA CAAGGTGTGC ACTGCGAGAT CAACATTGAC 
GACTGCACAC CGTTCACTGA CCCCATCACC CATGAGCCTA AATGCTTTAA CCAGGGCCGC 
TGTGTGGACC GTGTGGGCGG CTACCACTGC ATCTGTCCCC CTGGGTATGT TGGGGAACGC 
TGTGAGGGCG ACGTCAACGA GTGCCTGTCC AACCCCTGTG GCACACACAG CTGCATCCAG 
CTCAAAAACA ACTACCGCTG CGAGTGTCGC ACAGGATACA CAGGTCAGCA TTGTGACAAA 
GTGTTTGATG GCTGTAAAGG GAAGCCGTGT CGTAATGGAG GCACCTGCGC TGTAGCCAGT 
AACACTCCTC ATGGCTTTAT CTGCAAATGT CCACCGGGTT TTACGGGCTC GACCTGCGAG 
TACGACGCTC ACGCATGTGG GAGCCTTCAA TGCAAAAATG GCGGGACGTG CGTTTCTGGT 
CACAAGAGTC CCAAATGCCT GTGCACCCCT GCTTTCACTG GTCCCGAGTG CCAGGATCCC 
AGCGGAGGCC ACTGCACCAC TAACCCCTGC TATAACGGAG GCACGTGCGA GTATATAACC 
GAGGAGCCGT ATTACCACTG CATCTGTCCC ACCAACTTCA ACGGCCTCTT CTGCCACATC 
CTGGACTGGA GTTTTCCAGG CGGCACTGGT CAGGACATCA CGCCGGCTCC TAAGGTGTCG 
GTCAGCTGCG AGATTGAGCA GTGTAAAGTC AAGAAGGGCA ATAAGATCTG TGACAGCGCA 
TGTAATAATT ACGCCTGCGA TTGGGACGGT GGCGACTGTT CGCTGAACTT TAATGACCCA 
TGGAAGAACT GTTCGGCGGC TCTGCAGTGC TGGCGCTATT TTAATAACGG GAAGTGTGAT 
GAACAGTGCC ACAACACGGG ATGCCTCTAT GATGGGTTCG ACTGCCAGAG AGTGGAGGCA 
CAGTGCAATC CACTATACGA TCAGTATTGC AAGGACCACT TTGCAGACGG CTACTGTGAC 
CAGGGCTGCA ATAATGCAGA GTGTGAATGG GACGGCCTGG ACTGCGCTAA CGACACCCCC 
GAGAAGCTGG CTGCAGGACT GCTGGTTGTG GTGGTCCACA TTCACCCTGA TCAGCTCCGA 
AACAACTCGT TCGGGTTTCT GCGGGAGCTC AGCCGAGTGC TCCACACCAA CGTGGTTTTC 
CGGCGGGATA GCAAGGGGCA AGAAATGATC TACCCGTATT ACGGCAATGA GCAGGAACTG 
AAGAAACACA ACATCAAACG ATCACTAGAC GGCTGGAACG ATGCTTCTAG CGATGTCTTG 
AGCTCGATGA AAAACAGCAT TTACAATATA GTAGTGGAAG GAGGGAGAAA ACGCAGAGAA 
CTGGAGAAGA TACAAGTCAA AGGCTCCGTG GTTTATCTGG AGATTGACAA CCGGCAGTGC 
TACCAGCAAA CCTCCGAATG CTTCCAGAGC GCCAACGACG CGGCGGCGTT CCTCGGAGCG 
CTGGCTTCCA GCGGCAGTCT GAAAATGCCT TACGTCATCG AGGCCGTCAC AAGTGAGATC 
GATGGACCGT CGCCTGTAGA GCTCTATCCA GTCTACGTGG TTCTGGCAGG ATTGGCGCTG 
CTGGCCTTTG TCGCCATTGG GATGGTGGCG TCCCGTAAAC GGCGCCGGGA ACACGGACAA 
CTTTGGTTTC CGGAGGGTTT TAAGACCAGC GAGCCCAGCA AGAAGAAGAG GAGAGAGCCG 
GTCGGAGAAG ATTCAGTCGG TTTGAGGCCA CTTAAGAACT GTTCAGACAT TTCGTTAATG 
GACGACAACC AGAATGAATG GGGAGAGGAG GAACAGTCCG ACAGCAAACG CTTCAGGTCT 
GAGGAGCAGG CGATGCTGGA TTTAGACGAT CAGCCAGATC ACAGACAGTG GACGCAGCAG 
CATTTGGACG CAGCCGATCT GCGCATCCCA TCCATCGCTC CCACACCGCC TCAGGGCGAG 
ATAGAGAACG ACTGCATGGA CGTCAATGCC CGAGGACCAG ATGGCTTTAC TCCTCTGATG 
ATTGCGTCCT GCAGTGGAGG AGGGCTCGAA ACGGGAAATA GCGAGGAAGA GGAAGACGCT 
TCAGCAAACG TCATCAATGA TTTCATCTAT CAGGGCGCCA ACCTTCACAA CCAGACAGAC 
CGCACGGGTG AGACGGCTCT ACACCTGGCT GCCCGCTACG CCCGCTCAGA CGCCGCGAAA 
CGACTGCTGG AGGCCAGTGC TGATGCTAAC ATCCAGGACA ATATGGGCAG GACTCCATTA 
CATGCCGCTG TGGCTGCTGA TGCCCAAGGC GTCTTCCAGA TTTTGATACG GAACCGCGCC 
ACAGATCTAG ACGCCCGCAT GCATGATGGC ACCACACCTC TGATACTGGC CGCAAGACTG 
GCGGTCGAGG GCATGGTGGA GGAACTCATC AACTGCCATG CTGACGTTAA TGCCATTGAT 
GACTTTGGTA AATCTGCTCT TCACTGGGCA GCAGCTGTGA ACAATGTAGA TGCAGCGATG 
GTGTTGCTTA AGAATGGGGC AAATAAAGAC ATGCAGAACA ATAAGGAGGA GACTCCTCTT 
TTCCTGGCGG CAAGGGAAGG AAGTTACGAG ACGGCTAAAG TTCTTCTGGA GCATTTTGCA 
AACCGGGAAA TCACAGACCA CATGGATCGA CTCCCGCGAG ACATCGCGCA AGACAGAATG 
CATCATGATA TTGTGCGTCT TATTGATGAA TACAACCTAG TGCGCAGTCC TCCCATGCAC 
AGCGCCCCGC TCTGCACCAC GCTTTCCCCA CCCCTCTGCT CCCCCAATGG CTTCATGGGC 
AACATGAAGC CCTCGGTGCA AAGCAAAAAA CCTCGTAAGC CCAGCACTAA AGGCATCGGC 
TGCAAAGACG GCAAAGATAT GAAAGTCAAA AAGAAGAAAG CACAAGACGG AAAGGGAAAC 
TTATTAGACA GCTCGGCTGT TCTCTCTCCG GTGGATTCCC TAGAGTCGCC TCACGGGTAT 
ATTTCTGACA TCGCCTCGCC ACCCCAGATG ACATCACCCT TCCAACAATC GCCCTCCATG 
TCGCTCAATC AGCTACAGGG AATGTCAGAC AACCACATGG GCGTCAGCCA TCTTGGAATT 
GGCAACAACC AAGATCTTTC CCATATACAG TTTGATCCAT TGCCTCCACG TCTCACTCAT 
CTTCCAGTGG CCGGATCGAA CGGTTCAAAC GTCATGAACG GCCAATGCGA ATGGCTAGGG 
CGGATGCATG GAAGCATGGC TCCGCAAAAC CAGTTCACAG CCATGAGAAA CGCCTCAGGT 
CAAGCCAACC TTCACCAATC AGGCTTGATG ACGTCACATC ACAATGGCCG CCCTGCCACG 
CTCTCTCAAA TGATGAACTA TCAAAGCATG CAGAACACAC ATCTAATGCA GCAGATGCAG 
CAGAGCATGC AGCCGCGGCC CCAGCAGACC GGCGTCCAGC TGCAGAGCCA GAGCCAGAAC 
TTCATCGGGG GTGATCTGGG TGGACCGGAG CTCCAGCAGA GCGCAGGGAA CAGCATGTCC 
ATCCATACTA TAATCCCCCA GGAGACCCAG CTGCTCAACC CGTCCTCTCT GGGGTCCAGC 
ATGGCGGGCA CGCAGTTCCT GACGCCACCT TCCCAGCACA GTTACACTCC CGCATTGGAC 
GCCAACACTC CCAACCACCA ACTTCAAGTG CCTGACCACC ATCCTTTCCT CACGCCATCT 
CCCGGTTCTC CAGACCAGTG GTCCAGCTCG TCACCCAATT CCAACATGTC CGATTGGTCG 
GAGGGTATTT CCAGTCCTCC CACCAGCATG CAGTCACAAA TCGGACACAT GCCCGAGCAA 
TTCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168706.1
CDS	237..7685
Translation	MHLFFVKLIVVISLNTLTQGLECSEKCQNGGTCEPTADGRGECKCLDLYAGPACQFRNPCFQSPCRNGGVCRLITSANKVDFVCNCSLGYTDRLCLTPTNNVCLGAPCRNGGTCELTSIHNYRCKCPPGWSGKTCQQADPCASNPCANGGQCSPFDSDFLCHCTPYFSGQTCKQDVNECAQIPSPCKNGGVCENGVGTYHCNCPAEYTGKHCESLYQPCNPSPCLHGGTCVQKGETSYECSCLPGFSGQNCEENIDDCPDHRCLNGGTCVDGVNTYNCQCKPEWTGQFCTEDVNECDLMPNSCQNGGTCLNTQGGYNCVCVNGWTGDDCSENIDDCADAACHTGATCHDRVASFLCECPHGRTGLLCHLDDACISNPCQKGSNCDTNPVNGKAICTCPLGYVGPACDQDVDECSLGANPCEHAGKCINTKGSFQCKCLQGYVGARCELDINECLSTPCQNDATCLDQIGGFHCICMPGYEGVFCQINTDECASMPCLNNGKCIDKINNYQCECPTGFSGSQCQFDIDECASTPCKNGAKCMDGPNMYTCQCTEGYTGQHCETDVDECLSNPCHYGTCKDGLASFTCVCRAGFMGRLCEININECLSQPCQNGGTCQDRENAYLCVCPKGTAGANCEINLDDCQSNPCDFGRCIDKINGYECACEPGYTGKMCNINIDECAINPCHNGGTCVDGVNGFTCLCREGYHDTTCQSQLNECLSNPCIHGHCEDKVNGYNCICDSGWSGVNCDINNNECESNPCMNGGTCKDMTSGYVCTCRAGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGENCETLLAPCGSKPCKNGGVCKESEDYESFSCVCPAGWQGQTCEVDINECVKNPCRNDAICQNSIGSYKCSCKAGYTGRNCETDIDDCKPNPCSNGGFCKDAVNAFACTCLPGFRGGRCEEDINECESNPCKNGANCTDCVNSYTCTCPPGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPKGFTGNYCQHDINECDSRPCMNGGTCQDSYGTYKCTCPQGYHGLNCQELVNWCKPSPCKNGGICRQSGTRYSCQCQTGWTGLYCDVPSVSCEVAAKQQGVDVVRLCRNSGQCLDAGNTHYCHCQAGYTGSYCEEQVDECIPNPCQNGATCTDYLGGYSCECVPGYHGVNCSDEINECLSQPCQNGGTCIDLINTYKCSCPRGTQGVHCEINIDDCTPFTDPITHEPKCFNQGRCVDRVGGYHCICPPGYVGERCEGDVNECLSNPCGTHSCIQLKNNYRCECRTGYTGQHCDKVFDGCKGKPCRNGGTCAVASNTPHGFICKCPPGFTGSTCEYDAHACGSLQCKNGGTCVSGHKSPKCLCTPAFTGPECQDPSGGHCTTNPCYNGGTCEYITEEPYYHCICPTNFNGLFCHILDWSFPGGTGQDITPAPKVSVSCEIEQCKVKKGNKICDSACNNYACDWDGGDCSLNFNDPWKNCSAALQCWRYFNNGKCDEQCHNTGCLYDGFDCQRVEAQCNPLYDQYCKDHFADGYCDQGCNNAECEWDGLDCANDTPEKLAAGLLVVVVHIHPDQLRNNSFGFLRELSRVLHTNVVFRRDSKGQEMIYPYYGNEQELKKHNIKRSLDGWNDASSDVLSSMKNSIYNIVVEGGRKRRELEKIQVKGSVVYLEIDNRQCYQQTSECFQSANDAAAFLGALASSGSLKMPYVIEAVTSEIDGPSPVELYPVYVVLAGLALLAFVAIGMVASRKRRREHGQLWFPEGFKTSEPSKKKRREPVGEDSVGLRPLKNCSDISLMDDNQNEWGEEEQSDSKRFRSEEQAMLDLDDQPDHRQWTQQHLDAADLRIPSIAPTPPQGEIENDCMDVNARGPDGFTPLMIASCSGGGLETGNSEEEEDASANVINDFIYQGANLHNQTDRTGETALHLAARYARSDAAKRLLEASADANIQDNMGRTPLHAAVAADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMVEELINCHADVNAIDDFGKSALHWAAAVNNVDAAMVLLKNGANKDMQNNKEETPLFLAAREGSYETAKVLLEHFANREITDHMDRLPRDIAQDRMHHDIVRLIDEYNLVRSPPMHSAPLCTTLSPPLCSPNGFMGNMKPSVQSKKPRKPSTKGIGCKDGKDMKVKKKKAQDGKGNLLDSSAVLSPVDSLESPHGYISDIASPPQMTSPFQQSPSMSLNQLQGMSDNHMGVSHLGIGNNQDLSHIQFDPLPPRLTHLPVAGSNGSNVMNGQCEWLGRMHGSMAPQNQFTAMRNASGQANLHQSGLMTSHHNGRPATLSQMMNYQSMQNTHLMQQMQQSMQPRPQQTGVQLQSQSQNFIGGDLGGPELQQSAGNSMSIHTIIPQETQLLNPSSLGSSMAGTQFLTPPSQHSYTPALDANTPNHQLQVPDHHPFLTPSPGSPDQWSSSSPNSNMSDWSEGISSPPTSMQSQIGHMPEQFK

Target ORF information:

RefSeq Version	XM_005168649.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio notch 1b (notch1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168649.3

ORF Insert Sequence:

ATGCATCTTT TCTTCGTGAA ACTAATTGTT GTGATATCAC TCAATACTTT GACACAAGGC 
CTGGAATGTT CAGAAAAATG CCAAAACGGA GGCACATGTG AACCAACTGC AGACGGGAGA 
GGAGAATGCA AGTGTCTGGA TCTGTACGCC GGCCCAGCGT GCCAGTTCCG CAACCCTTGC 
TTCCAATCCC CGTGCAGAAA TGGTGGCGTG TGCCGTTTGA TCACTTCTGC CAACAAGGTG 
GACTTTGTCT GCAACTGCAG CCTGGGCTAC ACGGACCGAT TGTGCCTCAC TCCCACCAAC 
AACGTGTGCC TTGGTGCTCC GTGCCGAAAC GGAGGAACGT GCGAACTCAC CAGCATCCAC 
AACTACAGGT GTAAATGCCC ACCAGGCTGG TCAGGTAAAA CCTGCCAGCA AGCTGATCCC 
TGTGCCTCGA ATCCTTGTGC CAATGGTGGT CAGTGTAGCC CCTTTGATTC GGACTTCCTC 
TGCCACTGTA CACCTTACTT CTCAGGCCAA ACCTGCAAAC AAGATGTCAA CGAGTGCGCG 
CAGATTCCCT CTCCGTGTAA GAACGGTGGC GTGTGTGAGA ACGGAGTGGG CACGTATCAC 
TGCAACTGTC CCGCCGAGTA CACTGGGAAA CACTGCGAGA GCCTGTATCA GCCGTGTAAC 
CCCTCGCCAT GCTTACACGG GGGCACCTGC GTACAGAAGG GGGAGACCAG TTATGAGTGC 
TCCTGTCTGC CAGGTTTTAG TGGGCAGAAC TGCGAAGAAA ACATCGATGA TTGTCCAGAT 
CATCGCTGCC TTAATGGAGG GACCTGTGTG GATGGAGTGA ACACCTACAA TTGCCAGTGC 
AAACCAGAGT GGACAGGTCA GTTCTGTACC GAAGATGTCA ACGAGTGCGA TTTGATGCCC 
AACTCCTGCC AGAACGGTGG CACGTGCTTG AACACGCAGG GTGGGTATAA CTGTGTGTGC 
GTGAACGGCT GGACGGGGGA CGACTGCAGC GAGAACATCG ACGACTGCGC AGATGCAGCC 
TGTCATACTG GAGCCACATG CCACGATCGG GTGGCCTCCT TCCTCTGCGA ATGTCCTCAC 
GGGCGCACAG GTCTGTTGTG TCATCTAGAT GATGCTTGCA TTAGTAACCC GTGTCAAAAA 
GGCTCTAACT GTGACACCAA CCCAGTCAAC GGCAAAGCCA TCTGTACCTG TCCACTGGGT 
TATGTTGGGC CGGCCTGTGA CCAAGACGTT GATGAATGCT CACTGGGTGC AAACCCTTGT 
GAACATGCTG GAAAGTGTAT AAACACAAAA GGCTCGTTCC AGTGTAAATG TCTGCAGGGA 
TATGTTGGAG CTCGATGTGA GCTGGACATA AATGAGTGCC TTTCCACCCC GTGCCAAAAC 
GACGCCACCT GTTTAGATCA GATTGGAGGA TTCCATTGCA TCTGTATGCC AGGGTACGAG 
GGAGTCTTCT GTCAGATCAA TACCGATGAG TGCGCCAGCA TGCCCTGCCT CAACAATGGG 
AAGTGCATCG ACAAGATCAA CAACTACCAA TGCGAGTGCC CCACAGGATT TTCGGGGAGC 
CAATGCCAGT TCGACATTGA CGAGTGCGCT AGTACTCCTT GTAAAAATGG GGCCAAGTGT 
ATGGATGGGC CCAATATGTA CACCTGCCAG TGCACTGAAG GATACACAGG GCAGCACTGT 
GAGACAGATG TAGATGAGTG TCTGTCCAAC CCGTGTCACT ACGGCACCTG TAAAGATGGC 
CTGGCGTCCT TCACCTGCGT CTGCCGTGCG GGTTTCATGG GCCGTCTGTG CGAGATCAAC 
ATTAATGAGT GTCTCAGTCA GCCATGCCAA AACGGAGGCA CCTGCCAGGA CCGTGAGAAT 
GCCTACTTGT GTGTCTGCCC CAAAGGAACT GCAGGAGCTA ACTGTGAGAT CAACCTGGAT 
GACTGTCAGA GCAATCCCTG TGATTTCGGG AGATGCATCG ACAAGATAAA TGGGTACGAG 
TGTGCCTGCG AACCGGGATA CACAGGGAAA ATGTGCAACA TCAATATCGA TGAATGTGCC 
ATCAACCCCT GCCACAATGG CGGAACCTGT GTGGATGGAG TCAATGGCTT CACTTGTCTG 
TGCAGAGAGG GCTATCATGA CACCACCTGC CAATCACAGC TCAACGAATG CCTCAGCAAC 
CCCTGTATCC ATGGACACTG TGAAGACAAA GTCAATGGAT ATAACTGTAT TTGCGACTCT 
GGCTGGAGTG GAGTCAACTG TGACATTAAC AACAACGAGT GCGAGTCCAA CCCCTGTATG 
AATGGAGGCA CTTGTAAGGA CATGACCAGT GGCTACGTCT GCACATGTCG CGCCGGCTTC 
AGCGGTCCCA ATTGCCAAAC GAATATCAAC GAATGTGCCT CCAACCCATG CTTGAATCAA 
GGAACCTGCA TTGATGATGT GGCCGGATAC AAATGCAACT GTCTGCTTCC TTACACTGGC 
GAGAACTGTG AGACCCTGTT GGCCCCGTGC GGCTCTAAAC CCTGTAAGAA CGGAGGCGTT 
TGCAAGGAAT CGGAAGACTA CGAGAGCTTC TCTTGTGTGT GTCCTGCGGG ATGGCAAGGC 
CAAACATGTG AGGTGGACAT CAACGAGTGT GTTAAAAATC CTTGCCGAAA TGACGCCATC 
TGCCAAAACT CCATTGGCAG CTACAAGTGC AGTTGCAAAG CGGGCTACAC GGGTCGCAAC 
TGTGAGACAG ACATCGATGA CTGCAAGCCC AACCCCTGCA GTAACGGTGG CTTCTGCAAA 
GACGCAGTGA ACGCCTTCGC TTGCACCTGC CTGCCGGGTT TCAGGGGCGG CAGATGCGAG 
GAGGACATTA ACGAATGTGA GAGTAACCCG TGTAAAAACG GCGCCAACTG CACTGATTGT 
GTGAACAGCT ACACCTGCAC CTGTCCACCT GGATTCAGTG GGATTCACTG CGAGAACAAC 
ACACCTGACT GTACTGAGAG CTCTTGTTTT AATGGTGGCA CATGTGTGGA TGGCATCAAC 
AGCTTCACTT GCCTGTGCCC TAAAGGCTTC ACTGGCAACT ACTGCCAGCA TGACATCAAC 
GAGTGCGACT CCAGACCGTG CATGAATGGA GGCACCTGCC AGGACAGCTA TGGCACCTAC 
AAGTGCACCT GCCCTCAGGG ATACCACGGT CTTAACTGTC AGGAGCTGGT GAACTGGTGT 
AAACCGTCTC CCTGTAAGAA TGGAGGGATC TGCAGACAGA GCGGCACGAG ATACAGCTGT 
CAGTGTCAGA CAGGCTGGAC TGGTTTATAC TGTGACGTTC CCAGTGTTTC CTGCGAGGTG 
GCCGCCAAAC AGCAAGGTGT TGATGTGGTC CGGCTGTGTC GTAACTCTGG CCAGTGTCTG 
GACGCTGGAA ACACACACTA TTGTCACTGT CAGGCCGGAT ACACGGGCAG CTACTGTGAG 
GAGCAGGTGG ACGAATGCAT TCCCAATCCG TGCCAGAACG GAGCAACTTG CACCGACTAC 
CTGGGCGGAT ACTCCTGTGA ATGTGTGCCA GGTTATCATG GAGTGAACTG CTCTGATGAG 
ATCAATGAGT GTCTGTCTCA GCCCTGCCAG AACGGGGGCA CATGCATCGA CCTGATCAAT 
ACCTACAAAT GCTCCTGTCC TCGGGGAACA CAAGGTGTGC ACTGCGAGAT CAACATTGAC 
GACTGCACAC CGTTCACTGA CCCCATCACC CATGAGCCTA AATGCTTTAA CCAGGGCCGC 
TGTGTGGACC GTGTGGGCGG CTACCACTGC ATCTGTCCCC CTGGGTATGT TGGGGAACGC 
TGTGAGGGCG ACGTCAACGA GTGCCTGTCC AACCCCTGTG GCACACACAG CTGCATCCAG 
CTCAAAAACA ACTACCGCTG CGAGTGTCGC ACAGGATACA CAGGTCAGCA TTGTGACAAA 
GTGTTTGATG GCTGTAAAGG GAAGCCGTGT CGTAATGGAG GCACCTGCGC TGTAGCCAGT 
AACACTCCTC ATGGCTTTAT CTGCAAATGT CCACCGGGTT TTACGGGCTC GACCTGCGAG 
TACGACGCTC ACGCATGTGG GAGCCTTCAA TGCAAAAATG GCGGGACGTG CGTTTCTGGT 
CACAAGAGTC CCAAATGCCT GTGCACCCCT GCTTTCACTG GTCCCGAGTG CCAGGATCCC 
AGCGGAGGCC ACTGCACCAC TAACCCCTGC TATAACGGAG GCACGTGCGA GTATATAACC 
GAGGAGCCGT ATTACCACTG CATCTGTCCC ACCAACTTCA ACGGCCTCTT CTGCCACATC 
CTGGACTGGA GTTTTCCAGG CGGCACTGGT CAGGACATCA CGCCGGCTCC TAAGGTGTCG 
GTCAGCTGCG AGATTGAGCA GTGTAAAGTC AAGAAGGGCA ATAAGATCTG TGACAGCGCA 
TGTAATAATT ACGCCTGCGA TTGGGACGGT GGCGACTGTT CGCTGAACTT TAATGACCCA 
TGGAAGAACT GTTCGGCGGC TCTGCAGTGC TGGCGCTATT TTAATAACGG GAAGTGTGAT 
GAACAGTGCC ACAACACGGG ATGCCTCTAT GATGGGTTCG ACTGCCAGAG AGTGGAGGCA 
CAGTGCAATC CACTATACGA TCAGTATTGC AAGGACCACT TTGCAGACGG CTACTGTGAC 
CAGGGCTGCA ATAATGCAGA GTGTGAATGG GACGGCCTGG ACTGCGCTAA CGACACCCCC 
GAGAAGCTGG CTGCAGGACT GCTGGTTGTG GTGGTCCACA TTCACCCTGA TCAGCTCCGA 
AACAACTCGT TCGGGTTTCT GCGGGAGCTC AGCCGAGTGC TCCACACCAA CGTGGTTTTC 
CGGCGGGATA GCAAGGGGCA AGAAATGATC TACCCGTATT ACGGCAATGA GCAGGAACTG 
AAGAAACACA ACATCAAACG ATCACTAGAC GGCTGGAACG ATGCTTCTAG CGATGTCTTG 
AGCTCGATGA AAAACAGCAT TTACAATATA GTAGTGGAAG GAGGGAGAAA ACGCAGAGAA 
CTGGAGAAGA TACAAGTCAA AGGCTCCGTG GTTTATCTGG AGATTGACAA CCGGCAGTGC 
TACCAGCAAA CCTCCGAATG CTTCCAGAGC GCCAACGACG CGGCGGCGTT CCTCGGAGCG 
CTGGCTTCCA GCGGCAGTCT GAAAATGCCT TACGTCATCG AGGCCGTCAC AAGTGAGATC 
GATGGACCGT CGCCTGTAGA GCTCTATCCA GTCTACGTGG TTCTGGCAGG ATTGGCGCTG 
CTGGCCTTTG TCGCCATTGG GATGGTGGCG TCCCGTAAAC GGCGCCGGGA ACACGGACAA 
CTTTGGTTTC CGGAGGGTTT TAAGACCAGC GAGCCCAGCA AGAAGAAGAG GAGAGAGCCG 
GTCGGAGAAG ATTCAGTCGG TTTGAGGCCA CTTAAGAACT GTTCAGACAT TTCGTTAATG 
GACGACAACC AGAATGAATG GGGAGAGGAG GAACAGTCCG ACAGCAAACG CTTCAGGTCT 
GAGGAGCAGG CGATGCTGGA TTTAGACGAT CAGCCAGATC ACAGACAGTG GACGCAGCAG 
CATTTGGACG CAGCCGATCT GCGCATCCCA TCCATCGCTC CCACACCGCC TCAGGGCGAG 
ATAGAGAACG ACTGCATGGA CGTCAATGCC CGAGGACCAG ATGGCTTTAC TCCTCTGATG 
ATTGCGTCCT GCAGTGGAGG AGGGCTCGAA ACGGGAAATA GCGAGGAAGA GGAAGACGCT 
TCAGCAAACG TCATCAATGA TTTCATCTAT CAGGGCGCCA ACCTTCACAA CCAGACAGAC 
CGCACGGGTG AGACGGCTCT ACACCTGGCT GCCCGCTACG CCCGCTCAGA CGCCGCGAAA 
CGACTGCTGG AGGCCAGTGC TGATGCTAAC ATCCAGGACA ATATGGGCAG GACTCCATTA 
CATGCCGCTG TGGCTGCTGA TGCCCAAGGC GTCTTCCAGA TTTTGATACG GAACCGCGCC 
ACAGATCTAG ACGCCCGCAT GCATGATGGC ACCACACCTC TGATACTGGC CGCAAGACTG 
GCGGTCGAGG GCATGGTGGA GGAACTCATC AACTGCCATG CTGACGTTAA TGCCATTGAT 
GACTTTGGTA AATCTGCTCT TCACTGGGCA GCAGCTGTGA ACAATGTAGA TGCAGCGATG 
GTGTTGCTTA AGAATGGGGC AAATAAAGAC ATGCAGAACA ATAAGGAGGA GACTCCTCTT 
TTCCTGGCGG CAAGGGAAGG AAGTTACGAG ACGGCTAAAG TTCTTCTGGA GCATTTTGCA 
AACCGGGAAA TCACAGACCA CATGGATCGA CTCCCGCGAG ACATCGCGCA AGACAGAATG 
CATCATGATA TTGTGCGTCT TATTGATGAA TACAACCTAG TGCGCAGTCC TCCCATGCAC 
AGCGCCCCGC TCTGCACCAC GCTTTCCCCA CCCCTCTGCT CCCCCAATGG CTTCATGGGC 
AACATGAAGC CCTCGGTGCA AAGCAAAAAA CCTCGTAAGC CCAGCACTAA AGGCATCGGC 
TGCAAAGACG GCAAAGATAT GAAAGTCAAA AAGAAGAAAG CACAAGACGG AAAGGGAAAC 
TTATTAGACA GCTCGGCTGT TCTCTCTCCG GTGGATTCCC TAGAGTCGCC TCACGGGTAT 
ATTTCTGACA TCGCCTCGCC ACCCCAGATG ACATCACCCT TCCAACAATC GCCCTCCATG 
TCGCTCAATC AGCTACAGGG AATGTCAGAC AACCACATGG GCGTCAGCCA TCTTGGAATT 
GGCAACAACC AAGATCTTTC CCATATACAG TTTGATCCAT TGCCTCCACG TCTCACTCAT 
CTTCCAGTGG CCGGATCGAA CGGTTCAAAC GTCATGAACG GCCAATGCGA ATGGCTAGGG 
CGGATGCATG GAAGCATGGC TCCGCAAAAC CAGTTCACAG CCATGAGAAA CGCCTCAGGT 
CAAGCCAACC TTCACCAATC AGGCTTGATG ACGTCACATC ACAATGGCCG CCCTGCCACG 
CTCTCTCAAA TGATGAACTA TCAAAGCATG CAGAACACAC ATCTAATGCA GCAGATGCAG 
CAGAGCATGC AGCCGCGGCC CCAGCAGACC GGCGTCCAGC TGCAGAGCCA GAGCCAGAAC 
TTCATCGGGG GTGATCTGGG TGGACCGGAG CTCCAGCAGA GCGCAGGGAA CAGCATGTCC 
ATCCATACTA TAATCCCCCA GGAGACCCAG CTGCTCAACC CGTCCTCTCT GGGGTCCAGC 
ATGGCGGGCA CGCAGTTCCT GACGCCACCT TCCCAGCACA GTTACACTCC CGCATTGGAC 
GCCAACACTC CCAACCACCA ACTTCAAGTG CCTGACCACC ATCCTTTCCT CACGCCATCT 
CCCGGTTCTC CAGACCAGTG GTCCAGCTCG TCACCCAATT CCAACATGTC CGATTGGTCG 
GAGGGTATTT CCAGTCCTCC CACCAGCATG CAGTCACAAA TCGGACACAT GCCCGAGCAA 
TTCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

notch1b ( XM_005168649.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168706.1 Danio rerio notch 1b (notch1b), transcript variant X1, mRNA.

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CloneID	ODa11194
Clone ID Related Accession (Same CDS sequence)	XM_005168649.3 , XM_005168649.4
Accession Version	XM_005168649.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	neurogenic locus notch homolog protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168649.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCTTT TCTTCGTGAA ACTAATTGTT GTGATATCAC TCAATACTTT GACACAAGGC 
CTGGAATGTT CAGAAAAATG CCAAAACGGA GGCACATGTG AACCAACTGC AGACGGGAGA 
GGAGAATGCA AGTGTCTGGA TCTGTACGCC GGCCCAGCGT GCCAGTTCCG CAACCCTTGC 
TTCCAATCCC CGTGCAGAAA TGGTGGCGTG TGCCGTTTGA TCACTTCTGC CAACAAGGTG 
GACTTTGTCT GCAACTGCAG CCTGGGCTAC ACGGACCGAT TGTGCCTCAC TCCCACCAAC 
AACGTGTGCC TTGGTGCTCC GTGCCGAAAC GGAGGAACGT GCGAACTCAC CAGCATCCAC 
AACTACAGGT GTAAATGCCC ACCAGGCTGG TCAGGTAAAA CCTGCCAGCA AGCTGATCCC 
TGTGCCTCGA ATCCTTGTGC CAATGGTGGT CAGTGTAGCC CCTTTGATTC GGACTTCCTC 
TGCCACTGTA CACCTTACTT CTCAGGCCAA ACCTGCAAAC AAGATGTCAA CGAGTGCGCG 
CAGATTCCCT CTCCGTGTAA GAACGGTGGC GTGTGTGAGA ACGGAGTGGG CACGTATCAC 
TGCAACTGTC CCGCCGAGTA CACTGGGAAA CACTGCGAGA GCCTGTATCA GCCGTGTAAC 
CCCTCGCCAT GCTTACACGG GGGCACCTGC GTACAGAAGG GGGAGACCAG TTATGAGTGC 
TCCTGTCTGC CAGGTTTTAG TGGGCAGAAC TGCGAAGAAA ACATCGATGA TTGTCCAGAT 
CATCGCTGCC TTAATGGAGG GACCTGTGTG GATGGAGTGA ACACCTACAA TTGCCAGTGC 
AAACCAGAGT GGACAGGTCA GTTCTGTACC GAAGATGTCA ACGAGTGCGA TTTGATGCCC 
AACTCCTGCC AGAACGGTGG CACGTGCTTG AACACGCAGG GTGGGTATAA CTGTGTGTGC 
GTGAACGGCT GGACGGGGGA CGACTGCAGC GAGAACATCG ACGACTGCGC AGATGCAGCC 
TGTCATACTG GAGCCACATG CCACGATCGG GTGGCCTCCT TCCTCTGCGA ATGTCCTCAC 
GGGCGCACAG GTCTGTTGTG TCATCTAGAT GATGCTTGCA TTAGTAACCC GTGTCAAAAA 
GGCTCTAACT GTGACACCAA CCCAGTCAAC GGCAAAGCCA TCTGTACCTG TCCACTGGGT 
TATGTTGGGC CGGCCTGTGA CCAAGACGTT GATGAATGCT CACTGGGTGC AAACCCTTGT 
GAACATGCTG GAAAGTGTAT AAACACAAAA GGCTCGTTCC AGTGTAAATG TCTGCAGGGA 
TATGTTGGAG CTCGATGTGA GCTGGACATA AATGAGTGCC TTTCCACCCC GTGCCAAAAC 
GACGCCACCT GTTTAGATCA GATTGGAGGA TTCCATTGCA TCTGTATGCC AGGGTACGAG 
GGAGTCTTCT GTCAGATCAA TACCGATGAG TGCGCCAGCA TGCCCTGCCT CAACAATGGG 
AAGTGCATCG ACAAGATCAA CAACTACCAA TGCGAGTGCC CCACAGGATT TTCGGGGAGC 
CAATGCCAGT TCGACATTGA CGAGTGCGCT AGTACTCCTT GTAAAAATGG GGCCAAGTGT 
ATGGATGGGC CCAATATGTA CACCTGCCAG TGCACTGAAG GATACACAGG GCAGCACTGT 
GAGACAGATG TAGATGAGTG TCTGTCCAAC CCGTGTCACT ACGGCACCTG TAAAGATGGC 
CTGGCGTCCT TCACCTGCGT CTGCCGTGCG GGTTTCATGG GCCGTCTGTG CGAGATCAAC 
ATTAATGAGT GTCTCAGTCA GCCATGCCAA AACGGAGGCA CCTGCCAGGA CCGTGAGAAT 
GCCTACTTGT GTGTCTGCCC CAAAGGAACT GCAGGAGCTA ACTGTGAGAT CAACCTGGAT 
GACTGTCAGA GCAATCCCTG TGATTTCGGG AGATGCATCG ACAAGATAAA TGGGTACGAG 
TGTGCCTGCG AACCGGGATA CACAGGGAAA ATGTGCAACA TCAATATCGA TGAATGTGCC 
ATCAACCCCT GCCACAATGG CGGAACCTGT GTGGATGGAG TCAATGGCTT CACTTGTCTG 
TGCAGAGAGG GCTATCATGA CACCACCTGC CAATCACAGC TCAACGAATG CCTCAGCAAC 
CCCTGTATCC ATGGACACTG TGAAGACAAA GTCAATGGAT ATAACTGTAT TTGCGACTCT 
GGCTGGAGTG GAGTCAACTG TGACATTAAC AACAACGAGT GCGAGTCCAA CCCCTGTATG 
AATGGAGGCA CTTGTAAGGA CATGACCAGT GGCTACGTCT GCACATGTCG CGCCGGCTTC 
AGCGGTCCCA ATTGCCAAAC GAATATCAAC GAATGTGCCT CCAACCCATG CTTGAATCAA 
GGAACCTGCA TTGATGATGT GGCCGGATAC AAATGCAACT GTCTGCTTCC TTACACTGGC 
GAGAACTGTG AGACCCTGTT GGCCCCGTGC GGCTCTAAAC CCTGTAAGAA CGGAGGCGTT 
TGCAAGGAAT CGGAAGACTA CGAGAGCTTC TCTTGTGTGT GTCCTGCGGG ATGGCAAGGC 
CAAACATGTG AGGTGGACAT CAACGAGTGT GTTAAAAATC CTTGCCGAAA TGACGCCATC 
TGCCAAAACT CCATTGGCAG CTACAAGTGC AGTTGCAAAG CGGGCTACAC GGGTCGCAAC 
TGTGAGACAG ACATCGATGA CTGCAAGCCC AACCCCTGCA GTAACGGTGG CTTCTGCAAA 
GACGCAGTGA ACGCCTTCGC TTGCACCTGC CTGCCGGGTT TCAGGGGCGG CAGATGCGAG 
GAGGACATTA ACGAATGTGA GAGTAACCCG TGTAAAAACG GCGCCAACTG CACTGATTGT 
GTGAACAGCT ACACCTGCAC CTGTCCACCT GGATTCAGTG GGATTCACTG CGAGAACAAC 
ACACCTGACT GTACTGAGAG CTCTTGTTTT AATGGTGGCA CATGTGTGGA TGGCATCAAC 
AGCTTCACTT GCCTGTGCCC TAAAGGCTTC ACTGGCAACT ACTGCCAGCA TGACATCAAC 
GAGTGCGACT CCAGACCGTG CATGAATGGA GGCACCTGCC AGGACAGCTA TGGCACCTAC 
AAGTGCACCT GCCCTCAGGG ATACCACGGT CTTAACTGTC AGGAGCTGGT GAACTGGTGT 
AAACCGTCTC CCTGTAAGAA TGGAGGGATC TGCAGACAGA GCGGCACGAG ATACAGCTGT 
CAGTGTCAGA CAGGCTGGAC TGGTTTATAC TGTGACGTTC CCAGTGTTTC CTGCGAGGTG 
GCCGCCAAAC AGCAAGGTGT TGATGTGGTC CGGCTGTGTC GTAACTCTGG CCAGTGTCTG 
GACGCTGGAA ACACACACTA TTGTCACTGT CAGGCCGGAT ACACGGGCAG CTACTGTGAG 
GAGCAGGTGG ACGAATGCAT TCCCAATCCG TGCCAGAACG GAGCAACTTG CACCGACTAC 
CTGGGCGGAT ACTCCTGTGA ATGTGTGCCA GGTTATCATG GAGTGAACTG CTCTGATGAG 
ATCAATGAGT GTCTGTCTCA GCCCTGCCAG AACGGGGGCA CATGCATCGA CCTGATCAAT 
ACCTACAAAT GCTCCTGTCC TCGGGGAACA CAAGGTGTGC ACTGCGAGAT CAACATTGAC 
GACTGCACAC CGTTCACTGA CCCCATCACC CATGAGCCTA AATGCTTTAA CCAGGGCCGC 
TGTGTGGACC GTGTGGGCGG CTACCACTGC ATCTGTCCCC CTGGGTATGT TGGGGAACGC 
TGTGAGGGCG ACGTCAACGA GTGCCTGTCC AACCCCTGTG GCACACACAG CTGCATCCAG 
CTCAAAAACA ACTACCGCTG CGAGTGTCGC ACAGGATACA CAGGTCAGCA TTGTGACAAA 
GTGTTTGATG GCTGTAAAGG GAAGCCGTGT CGTAATGGAG GCACCTGCGC TGTAGCCAGT 
AACACTCCTC ATGGCTTTAT CTGCAAATGT CCACCGGGTT TTACGGGCTC GACCTGCGAG 
TACGACGCTC ACGCATGTGG GAGCCTTCAA TGCAAAAATG GCGGGACGTG CGTTTCTGGT 
CACAAGAGTC CCAAATGCCT GTGCACCCCT GCTTTCACTG GTCCCGAGTG CCAGGATCCC 
AGCGGAGGCC ACTGCACCAC TAACCCCTGC TATAACGGAG GCACGTGCGA GTATATAACC 
GAGGAGCCGT ATTACCACTG CATCTGTCCC ACCAACTTCA ACGGCCTCTT CTGCCACATC 
CTGGACTGGA GTTTTCCAGG CGGCACTGGT CAGGACATCA CGCCGGCTCC TAAGGTGTCG 
GTCAGCTGCG AGATTGAGCA GTGTAAAGTC AAGAAGGGCA ATAAGATCTG TGACAGCGCA 
TGTAATAATT ACGCCTGCGA TTGGGACGGT GGCGACTGTT CGCTGAACTT TAATGACCCA 
TGGAAGAACT GTTCGGCGGC TCTGCAGTGC TGGCGCTATT TTAATAACGG GAAGTGTGAT 
GAACAGTGCC ACAACACGGG ATGCCTCTAT GATGGGTTCG ACTGCCAGAG AGTGGAGGCA 
CAGTGCAATC CACTATACGA TCAGTATTGC AAGGACCACT TTGCAGACGG CTACTGTGAC 
CAGGGCTGCA ATAATGCAGA GTGTGAATGG GACGGCCTGG ACTGCGCTAA CGACACCCCC 
GAGAAGCTGG CTGCAGGACT GCTGGTTGTG GTGGTCCACA TTCACCCTGA TCAGCTCCGA 
AACAACTCGT TCGGGTTTCT GCGGGAGCTC AGCCGAGTGC TCCACACCAA CGTGGTTTTC 
CGGCGGGATA GCAAGGGGCA AGAAATGATC TACCCGTATT ACGGCAATGA GCAGGAACTG 
AAGAAACACA ACATCAAACG ATCACTAGAC GGCTGGAACG ATGCTTCTAG CGATGTCTTG 
AGCTCGATGA AAAACAGCAT TTACAATATA GTAGTGGAAG GAGGGAGAAA ACGCAGAGAA 
CTGGAGAAGA TACAAGTCAA AGGCTCCGTG GTTTATCTGG AGATTGACAA CCGGCAGTGC 
TACCAGCAAA CCTCCGAATG CTTCCAGAGC GCCAACGACG CGGCGGCGTT CCTCGGAGCG 
CTGGCTTCCA GCGGCAGTCT GAAAATGCCT TACGTCATCG AGGCCGTCAC AAGTGAGATC 
GATGGACCGT CGCCTGTAGA GCTCTATCCA GTCTACGTGG TTCTGGCAGG ATTGGCGCTG 
CTGGCCTTTG TCGCCATTGG GATGGTGGCG TCCCGTAAAC GGCGCCGGGA ACACGGACAA 
CTTTGGTTTC CGGAGGGTTT TAAGACCAGC GAGCCCAGCA AGAAGAAGAG GAGAGAGCCG 
GTCGGAGAAG ATTCAGTCGG TTTGAGGCCA CTTAAGAACT GTTCAGACAT TTCGTTAATG 
GACGACAACC AGAATGAATG GGGAGAGGAG GAACAGTCCG ACAGCAAACG CTTCAGGTCT 
GAGGAGCAGG CGATGCTGGA TTTAGACGAT CAGCCAGATC ACAGACAGTG GACGCAGCAG 
CATTTGGACG CAGCCGATCT GCGCATCCCA TCCATCGCTC CCACACCGCC TCAGGGCGAG 
ATAGAGAACG ACTGCATGGA CGTCAATGCC CGAGGACCAG ATGGCTTTAC TCCTCTGATG 
ATTGCGTCCT GCAGTGGAGG AGGGCTCGAA ACGGGAAATA GCGAGGAAGA GGAAGACGCT 
TCAGCAAACG TCATCAATGA TTTCATCTAT CAGGGCGCCA ACCTTCACAA CCAGACAGAC 
CGCACGGGTG AGACGGCTCT ACACCTGGCT GCCCGCTACG CCCGCTCAGA CGCCGCGAAA 
CGACTGCTGG AGGCCAGTGC TGATGCTAAC ATCCAGGACA ATATGGGCAG GACTCCATTA 
CATGCCGCTG TGGCTGCTGA TGCCCAAGGC GTCTTCCAGA TTTTGATACG GAACCGCGCC 
ACAGATCTAG ACGCCCGCAT GCATGATGGC ACCACACCTC TGATACTGGC CGCAAGACTG 
GCGGTCGAGG GCATGGTGGA GGAACTCATC AACTGCCATG CTGACGTTAA TGCCATTGAT 
GACTTTGGTA AATCTGCTCT TCACTGGGCA GCAGCTGTGA ACAATGTAGA TGCAGCGATG 
GTGTTGCTTA AGAATGGGGC AAATAAAGAC ATGCAGAACA ATAAGGAGGA GACTCCTCTT 
TTCCTGGCGG CAAGGGAAGG AAGTTACGAG ACGGCTAAAG TTCTTCTGGA GCATTTTGCA 
AACCGGGAAA TCACAGACCA CATGGATCGA CTCCCGCGAG ACATCGCGCA AGACAGAATG 
CATCATGATA TTGTGCGTCT TATTGATGAA TACAACCTAG TGCGCAGTCC TCCCATGCAC 
AGCGCCCCGC TCTGCACCAC GCTTTCCCCA CCCCTCTGCT CCCCCAATGG CTTCATGGGC 
AACATGAAGC CCTCGGTGCA AAGCAAAAAA CCTCGTAAGC CCAGCACTAA AGGCATCGGC 
TGCAAAGACG GCAAAGATAT GAAAGTCAAA AAGAAGAAAG CACAAGACGG AAAGGGAAAC 
TTATTAGACA GCTCGGCTGT TCTCTCTCCG GTGGATTCCC TAGAGTCGCC TCACGGGTAT 
ATTTCTGACA TCGCCTCGCC ACCCCAGATG ACATCACCCT TCCAACAATC GCCCTCCATG 
TCGCTCAATC AGCTACAGGG AATGTCAGAC AACCACATGG GCGTCAGCCA TCTTGGAATT 
GGCAACAACC AAGATCTTTC CCATATACAG TTTGATCCAT TGCCTCCACG TCTCACTCAT 
CTTCCAGTGG CCGGATCGAA CGGTTCAAAC GTCATGAACG GCCAATGCGA ATGGCTAGGG 
CGGATGCATG GAAGCATGGC TCCGCAAAAC CAGTTCACAG CCATGAGAAA CGCCTCAGGT 
CAAGCCAACC TTCACCAATC AGGCTTGATG ACGTCACATC ACAATGGCCG CCCTGCCACG 
CTCTCTCAAA TGATGAACTA TCAAAGCATG CAGAACACAC ATCTAATGCA GCAGATGCAG 
CAGAGCATGC AGCCGCGGCC CCAGCAGACC GGCGTCCAGC TGCAGAGCCA GAGCCAGAAC 
TTCATCGGGG GTGATCTGGG TGGACCGGAG CTCCAGCAGA GCGCAGGGAA CAGCATGTCC 
ATCCATACTA TAATCCCCCA GGAGACCCAG CTGCTCAACC CGTCCTCTCT GGGGTCCAGC 
ATGGCGGGCA CGCAGTTCCT GACGCCACCT TCCCAGCACA GTTACACTCC CGCATTGGAC 
GCCAACACTC CCAACCACCA ACTTCAAGTG CCTGACCACC ATCCTTTCCT CACGCCATCT 
CCCGGTTCTC CAGACCAGTG GTCCAGCTCG TCACCCAATT CCAACATGTC CGATTGGTCG 
GAGGGTATTT CCAGTCCTCC CACCAGCATG CAGTCACAAA TCGGACACAT GCCCGAGCAA 
TTCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168706.1
CDS	239..7687
Translation	MHLFFVKLIVVISLNTLTQGLECSEKCQNGGTCEPTADGRGECKCLDLYAGPACQFRNPCFQSPCRNGGVCRLITSANKVDFVCNCSLGYTDRLCLTPTNNVCLGAPCRNGGTCELTSIHNYRCKCPPGWSGKTCQQADPCASNPCANGGQCSPFDSDFLCHCTPYFSGQTCKQDVNECAQIPSPCKNGGVCENGVGTYHCNCPAEYTGKHCESLYQPCNPSPCLHGGTCVQKGETSYECSCLPGFSGQNCEENIDDCPDHRCLNGGTCVDGVNTYNCQCKPEWTGQFCTEDVNECDLMPNSCQNGGTCLNTQGGYNCVCVNGWTGDDCSENIDDCADAACHTGATCHDRVASFLCECPHGRTGLLCHLDDACISNPCQKGSNCDTNPVNGKAICTCPLGYVGPACDQDVDECSLGANPCEHAGKCINTKGSFQCKCLQGYVGARCELDINECLSTPCQNDATCLDQIGGFHCICMPGYEGVFCQINTDECASMPCLNNGKCIDKINNYQCECPTGFSGSQCQFDIDECASTPCKNGAKCMDGPNMYTCQCTEGYTGQHCETDVDECLSNPCHYGTCKDGLASFTCVCRAGFMGRLCEININECLSQPCQNGGTCQDRENAYLCVCPKGTAGANCEINLDDCQSNPCDFGRCIDKINGYECACEPGYTGKMCNINIDECAINPCHNGGTCVDGVNGFTCLCREGYHDTTCQSQLNECLSNPCIHGHCEDKVNGYNCICDSGWSGVNCDINNNECESNPCMNGGTCKDMTSGYVCTCRAGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGENCETLLAPCGSKPCKNGGVCKESEDYESFSCVCPAGWQGQTCEVDINECVKNPCRNDAICQNSIGSYKCSCKAGYTGRNCETDIDDCKPNPCSNGGFCKDAVNAFACTCLPGFRGGRCEEDINECESNPCKNGANCTDCVNSYTCTCPPGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPKGFTGNYCQHDINECDSRPCMNGGTCQDSYGTYKCTCPQGYHGLNCQELVNWCKPSPCKNGGICRQSGTRYSCQCQTGWTGLYCDVPSVSCEVAAKQQGVDVVRLCRNSGQCLDAGNTHYCHCQAGYTGSYCEEQVDECIPNPCQNGATCTDYLGGYSCECVPGYHGVNCSDEINECLSQPCQNGGTCIDLINTYKCSCPRGTQGVHCEINIDDCTPFTDPITHEPKCFNQGRCVDRVGGYHCICPPGYVGERCEGDVNECLSNPCGTHSCIQLKNNYRCECRTGYTGQHCDKVFDGCKGKPCRNGGTCAVASNTPHGFICKCPPGFTGSTCEYDAHACGSLQCKNGGTCVSGHKSPKCLCTPAFTGPECQDPSGGHCTTNPCYNGGTCEYITEEPYYHCICPTNFNGLFCHILDWSFPGGTGQDITPAPKVSVSCEIEQCKVKKGNKICDSACNNYACDWDGGDCSLNFNDPWKNCSAALQCWRYFNNGKCDEQCHNTGCLYDGFDCQRVEAQCNPLYDQYCKDHFADGYCDQGCNNAECEWDGLDCANDTPEKLAAGLLVVVVHIHPDQLRNNSFGFLRELSRVLHTNVVFRRDSKGQEMIYPYYGNEQELKKHNIKRSLDGWNDASSDVLSSMKNSIYNIVVEGGRKRRELEKIQVKGSVVYLEIDNRQCYQQTSECFQSANDAAAFLGALASSGSLKMPYVIEAVTSEIDGPSPVELYPVYVVLAGLALLAFVAIGMVASRKRRREHGQLWFPEGFKTSEPSKKKRREPVGEDSVGLRPLKNCSDISLMDDNQNEWGEEEQSDSKRFRSEEQAMLDLDDQPDHRQWTQQHLDAADLRIPSIAPTPPQGEIENDCMDVNARGPDGFTPLMIASCSGGGLETGNSEEEEDASANVINDFIYQGANLHNQTDRTGETALHLAARYARSDAAKRLLEASADANIQDNMGRTPLHAAVAADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMVEELINCHADVNAIDDFGKSALHWAAAVNNVDAAMVLLKNGANKDMQNNKEETPLFLAAREGSYETAKVLLEHFANREITDHMDRLPRDIAQDRMHHDIVRLIDEYNLVRSPPMHSAPLCTTLSPPLCSPNGFMGNMKPSVQSKKPRKPSTKGIGCKDGKDMKVKKKKAQDGKGNLLDSSAVLSPVDSLESPHGYISDIASPPQMTSPFQQSPSMSLNQLQGMSDNHMGVSHLGIGNNQDLSHIQFDPLPPRLTHLPVAGSNGSNVMNGQCEWLGRMHGSMAPQNQFTAMRNASGQANLHQSGLMTSHHNGRPATLSQMMNYQSMQNTHLMQQMQQSMQPRPQQTGVQLQSQSQNFIGGDLGGPELQQSAGNSMSIHTIIPQETQLLNPSSLGSSMAGTQFLTPPSQHSYTPALDANTPNHQLQVPDHHPFLTPSPGSPDQWSSSSPNSNMSDWSEGISSPPTSMQSQIGHMPEQFK

Target ORF information:

RefSeq Version	XM_005168649.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio notch 1b (notch1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168649.4

ORF Insert Sequence:

ATGCATCTTT TCTTCGTGAA ACTAATTGTT GTGATATCAC TCAATACTTT GACACAAGGC 
CTGGAATGTT CAGAAAAATG CCAAAACGGA GGCACATGTG AACCAACTGC AGACGGGAGA 
GGAGAATGCA AGTGTCTGGA TCTGTACGCC GGCCCAGCGT GCCAGTTCCG CAACCCTTGC 
TTCCAATCCC CGTGCAGAAA TGGTGGCGTG TGCCGTTTGA TCACTTCTGC CAACAAGGTG 
GACTTTGTCT GCAACTGCAG CCTGGGCTAC ACGGACCGAT TGTGCCTCAC TCCCACCAAC 
AACGTGTGCC TTGGTGCTCC GTGCCGAAAC GGAGGAACGT GCGAACTCAC CAGCATCCAC 
AACTACAGGT GTAAATGCCC ACCAGGCTGG TCAGGTAAAA CCTGCCAGCA AGCTGATCCC 
TGTGCCTCGA ATCCTTGTGC CAATGGTGGT CAGTGTAGCC CCTTTGATTC GGACTTCCTC 
TGCCACTGTA CACCTTACTT CTCAGGCCAA ACCTGCAAAC AAGATGTCAA CGAGTGCGCG 
CAGATTCCCT CTCCGTGTAA GAACGGTGGC GTGTGTGAGA ACGGAGTGGG CACGTATCAC 
TGCAACTGTC CCGCCGAGTA CACTGGGAAA CACTGCGAGA GCCTGTATCA GCCGTGTAAC 
CCCTCGCCAT GCTTACACGG GGGCACCTGC GTACAGAAGG GGGAGACCAG TTATGAGTGC 
TCCTGTCTGC CAGGTTTTAG TGGGCAGAAC TGCGAAGAAA ACATCGATGA TTGTCCAGAT 
CATCGCTGCC TTAATGGAGG GACCTGTGTG GATGGAGTGA ACACCTACAA TTGCCAGTGC 
AAACCAGAGT GGACAGGTCA GTTCTGTACC GAAGATGTCA ACGAGTGCGA TTTGATGCCC 
AACTCCTGCC AGAACGGTGG CACGTGCTTG AACACGCAGG GTGGGTATAA CTGTGTGTGC 
GTGAACGGCT GGACGGGGGA CGACTGCAGC GAGAACATCG ACGACTGCGC AGATGCAGCC 
TGTCATACTG GAGCCACATG CCACGATCGG GTGGCCTCCT TCCTCTGCGA ATGTCCTCAC 
GGGCGCACAG GTCTGTTGTG TCATCTAGAT GATGCTTGCA TTAGTAACCC GTGTCAAAAA 
GGCTCTAACT GTGACACCAA CCCAGTCAAC GGCAAAGCCA TCTGTACCTG TCCACTGGGT 
TATGTTGGGC CGGCCTGTGA CCAAGACGTT GATGAATGCT CACTGGGTGC AAACCCTTGT 
GAACATGCTG GAAAGTGTAT AAACACAAAA GGCTCGTTCC AGTGTAAATG TCTGCAGGGA 
TATGTTGGAG CTCGATGTGA GCTGGACATA AATGAGTGCC TTTCCACCCC GTGCCAAAAC 
GACGCCACCT GTTTAGATCA GATTGGAGGA TTCCATTGCA TCTGTATGCC AGGGTACGAG 
GGAGTCTTCT GTCAGATCAA TACCGATGAG TGCGCCAGCA TGCCCTGCCT CAACAATGGG 
AAGTGCATCG ACAAGATCAA CAACTACCAA TGCGAGTGCC CCACAGGATT TTCGGGGAGC 
CAATGCCAGT TCGACATTGA CGAGTGCGCT AGTACTCCTT GTAAAAATGG GGCCAAGTGT 
ATGGATGGGC CCAATATGTA CACCTGCCAG TGCACTGAAG GATACACAGG GCAGCACTGT 
GAGACAGATG TAGATGAGTG TCTGTCCAAC CCGTGTCACT ACGGCACCTG TAAAGATGGC 
CTGGCGTCCT TCACCTGCGT CTGCCGTGCG GGTTTCATGG GCCGTCTGTG CGAGATCAAC 
ATTAATGAGT GTCTCAGTCA GCCATGCCAA AACGGAGGCA CCTGCCAGGA CCGTGAGAAT 
GCCTACTTGT GTGTCTGCCC CAAAGGAACT GCAGGAGCTA ACTGTGAGAT CAACCTGGAT 
GACTGTCAGA GCAATCCCTG TGATTTCGGG AGATGCATCG ACAAGATAAA TGGGTACGAG 
TGTGCCTGCG AACCGGGATA CACAGGGAAA ATGTGCAACA TCAATATCGA TGAATGTGCC 
ATCAACCCCT GCCACAATGG CGGAACCTGT GTGGATGGAG TCAATGGCTT CACTTGTCTG 
TGCAGAGAGG GCTATCATGA CACCACCTGC CAATCACAGC TCAACGAATG CCTCAGCAAC 
CCCTGTATCC ATGGACACTG TGAAGACAAA GTCAATGGAT ATAACTGTAT TTGCGACTCT 
GGCTGGAGTG GAGTCAACTG TGACATTAAC AACAACGAGT GCGAGTCCAA CCCCTGTATG 
AATGGAGGCA CTTGTAAGGA CATGACCAGT GGCTACGTCT GCACATGTCG CGCCGGCTTC 
AGCGGTCCCA ATTGCCAAAC GAATATCAAC GAATGTGCCT CCAACCCATG CTTGAATCAA 
GGAACCTGCA TTGATGATGT GGCCGGATAC AAATGCAACT GTCTGCTTCC TTACACTGGC 
GAGAACTGTG AGACCCTGTT GGCCCCGTGC GGCTCTAAAC CCTGTAAGAA CGGAGGCGTT 
TGCAAGGAAT CGGAAGACTA CGAGAGCTTC TCTTGTGTGT GTCCTGCGGG ATGGCAAGGC 
CAAACATGTG AGGTGGACAT CAACGAGTGT GTTAAAAATC CTTGCCGAAA TGACGCCATC 
TGCCAAAACT CCATTGGCAG CTACAAGTGC AGTTGCAAAG CGGGCTACAC GGGTCGCAAC 
TGTGAGACAG ACATCGATGA CTGCAAGCCC AACCCCTGCA GTAACGGTGG CTTCTGCAAA 
GACGCAGTGA ACGCCTTCGC TTGCACCTGC CTGCCGGGTT TCAGGGGCGG CAGATGCGAG 
GAGGACATTA ACGAATGTGA GAGTAACCCG TGTAAAAACG GCGCCAACTG CACTGATTGT 
GTGAACAGCT ACACCTGCAC CTGTCCACCT GGATTCAGTG GGATTCACTG CGAGAACAAC 
ACACCTGACT GTACTGAGAG CTCTTGTTTT AATGGTGGCA CATGTGTGGA TGGCATCAAC 
AGCTTCACTT GCCTGTGCCC TAAAGGCTTC ACTGGCAACT ACTGCCAGCA TGACATCAAC 
GAGTGCGACT CCAGACCGTG CATGAATGGA GGCACCTGCC AGGACAGCTA TGGCACCTAC 
AAGTGCACCT GCCCTCAGGG ATACCACGGT CTTAACTGTC AGGAGCTGGT GAACTGGTGT 
AAACCGTCTC CCTGTAAGAA TGGAGGGATC TGCAGACAGA GCGGCACGAG ATACAGCTGT 
CAGTGTCAGA CAGGCTGGAC TGGTTTATAC TGTGACGTTC CCAGTGTTTC CTGCGAGGTG 
GCCGCCAAAC AGCAAGGTGT TGATGTGGTC CGGCTGTGTC GTAACTCTGG CCAGTGTCTG 
GACGCTGGAA ACACACACTA TTGTCACTGT CAGGCCGGAT ACACGGGCAG CTACTGTGAG 
GAGCAGGTGG ACGAATGCAT TCCCAATCCG TGCCAGAACG GAGCAACTTG CACCGACTAC 
CTGGGCGGAT ACTCCTGTGA ATGTGTGCCA GGTTATCATG GAGTGAACTG CTCTGATGAG 
ATCAATGAGT GTCTGTCTCA GCCCTGCCAG AACGGGGGCA CATGCATCGA CCTGATCAAT 
ACCTACAAAT GCTCCTGTCC TCGGGGAACA CAAGGTGTGC ACTGCGAGAT CAACATTGAC 
GACTGCACAC CGTTCACTGA CCCCATCACC CATGAGCCTA AATGCTTTAA CCAGGGCCGC 
TGTGTGGACC GTGTGGGCGG CTACCACTGC ATCTGTCCCC CTGGGTATGT TGGGGAACGC 
TGTGAGGGCG ACGTCAACGA GTGCCTGTCC AACCCCTGTG GCACACACAG CTGCATCCAG 
CTCAAAAACA ACTACCGCTG CGAGTGTCGC ACAGGATACA CAGGTCAGCA TTGTGACAAA 
GTGTTTGATG GCTGTAAAGG GAAGCCGTGT CGTAATGGAG GCACCTGCGC TGTAGCCAGT 
AACACTCCTC ATGGCTTTAT CTGCAAATGT CCACCGGGTT TTACGGGCTC GACCTGCGAG 
TACGACGCTC ACGCATGTGG GAGCCTTCAA TGCAAAAATG GCGGGACGTG CGTTTCTGGT 
CACAAGAGTC CCAAATGCCT GTGCACCCCT GCTTTCACTG GTCCCGAGTG CCAGGATCCC 
AGCGGAGGCC ACTGCACCAC TAACCCCTGC TATAACGGAG GCACGTGCGA GTATATAACC 
GAGGAGCCGT ATTACCACTG CATCTGTCCC ACCAACTTCA ACGGCCTCTT CTGCCACATC 
CTGGACTGGA GTTTTCCAGG CGGCACTGGT CAGGACATCA CGCCGGCTCC TAAGGTGTCG 
GTCAGCTGCG AGATTGAGCA GTGTAAAGTC AAGAAGGGCA ATAAGATCTG TGACAGCGCA 
TGTAATAATT ACGCCTGCGA TTGGGACGGT GGCGACTGTT CGCTGAACTT TAATGACCCA 
TGGAAGAACT GTTCGGCGGC TCTGCAGTGC TGGCGCTATT TTAATAACGG GAAGTGTGAT 
GAACAGTGCC ACAACACGGG ATGCCTCTAT GATGGGTTCG ACTGCCAGAG AGTGGAGGCA 
CAGTGCAATC CACTATACGA TCAGTATTGC AAGGACCACT TTGCAGACGG CTACTGTGAC 
CAGGGCTGCA ATAATGCAGA GTGTGAATGG GACGGCCTGG ACTGCGCTAA CGACACCCCC 
GAGAAGCTGG CTGCAGGACT GCTGGTTGTG GTGGTCCACA TTCACCCTGA TCAGCTCCGA 
AACAACTCGT TCGGGTTTCT GCGGGAGCTC AGCCGAGTGC TCCACACCAA CGTGGTTTTC 
CGGCGGGATA GCAAGGGGCA AGAAATGATC TACCCGTATT ACGGCAATGA GCAGGAACTG 
AAGAAACACA ACATCAAACG ATCACTAGAC GGCTGGAACG ATGCTTCTAG CGATGTCTTG 
AGCTCGATGA AAAACAGCAT TTACAATATA GTAGTGGAAG GAGGGAGAAA ACGCAGAGAA 
CTGGAGAAGA TACAAGTCAA AGGCTCCGTG GTTTATCTGG AGATTGACAA CCGGCAGTGC 
TACCAGCAAA CCTCCGAATG CTTCCAGAGC GCCAACGACG CGGCGGCGTT CCTCGGAGCG 
CTGGCTTCCA GCGGCAGTCT GAAAATGCCT TACGTCATCG AGGCCGTCAC AAGTGAGATC 
GATGGACCGT CGCCTGTAGA GCTCTATCCA GTCTACGTGG TTCTGGCAGG ATTGGCGCTG 
CTGGCCTTTG TCGCCATTGG GATGGTGGCG TCCCGTAAAC GGCGCCGGGA ACACGGACAA 
CTTTGGTTTC CGGAGGGTTT TAAGACCAGC GAGCCCAGCA AGAAGAAGAG GAGAGAGCCG 
GTCGGAGAAG ATTCAGTCGG TTTGAGGCCA CTTAAGAACT GTTCAGACAT TTCGTTAATG 
GACGACAACC AGAATGAATG GGGAGAGGAG GAACAGTCCG ACAGCAAACG CTTCAGGTCT 
GAGGAGCAGG CGATGCTGGA TTTAGACGAT CAGCCAGATC ACAGACAGTG GACGCAGCAG 
CATTTGGACG CAGCCGATCT GCGCATCCCA TCCATCGCTC CCACACCGCC TCAGGGCGAG 
ATAGAGAACG ACTGCATGGA CGTCAATGCC CGAGGACCAG ATGGCTTTAC TCCTCTGATG 
ATTGCGTCCT GCAGTGGAGG AGGGCTCGAA ACGGGAAATA GCGAGGAAGA GGAAGACGCT 
TCAGCAAACG TCATCAATGA TTTCATCTAT CAGGGCGCCA ACCTTCACAA CCAGACAGAC 
CGCACGGGTG AGACGGCTCT ACACCTGGCT GCCCGCTACG CCCGCTCAGA CGCCGCGAAA 
CGACTGCTGG AGGCCAGTGC TGATGCTAAC ATCCAGGACA ATATGGGCAG GACTCCATTA 
CATGCCGCTG TGGCTGCTGA TGCCCAAGGC GTCTTCCAGA TTTTGATACG GAACCGCGCC 
ACAGATCTAG ACGCCCGCAT GCATGATGGC ACCACACCTC TGATACTGGC CGCAAGACTG 
GCGGTCGAGG GCATGGTGGA GGAACTCATC AACTGCCATG CTGACGTTAA TGCCATTGAT 
GACTTTGGTA AATCTGCTCT TCACTGGGCA GCAGCTGTGA ACAATGTAGA TGCAGCGATG 
GTGTTGCTTA AGAATGGGGC AAATAAAGAC ATGCAGAACA ATAAGGAGGA GACTCCTCTT 
TTCCTGGCGG CAAGGGAAGG AAGTTACGAG ACGGCTAAAG TTCTTCTGGA GCATTTTGCA 
AACCGGGAAA TCACAGACCA CATGGATCGA CTCCCGCGAG ACATCGCGCA AGACAGAATG 
CATCATGATA TTGTGCGTCT TATTGATGAA TACAACCTAG TGCGCAGTCC TCCCATGCAC 
AGCGCCCCGC TCTGCACCAC GCTTTCCCCA CCCCTCTGCT CCCCCAATGG CTTCATGGGC 
AACATGAAGC CCTCGGTGCA AAGCAAAAAA CCTCGTAAGC CCAGCACTAA AGGCATCGGC 
TGCAAAGACG GCAAAGATAT GAAAGTCAAA AAGAAGAAAG CACAAGACGG AAAGGGAAAC 
TTATTAGACA GCTCGGCTGT TCTCTCTCCG GTGGATTCCC TAGAGTCGCC TCACGGGTAT 
ATTTCTGACA TCGCCTCGCC ACCCCAGATG ACATCACCCT TCCAACAATC GCCCTCCATG 
TCGCTCAATC AGCTACAGGG AATGTCAGAC AACCACATGG GCGTCAGCCA TCTTGGAATT 
GGCAACAACC AAGATCTTTC CCATATACAG TTTGATCCAT TGCCTCCACG TCTCACTCAT 
CTTCCAGTGG CCGGATCGAA CGGTTCAAAC GTCATGAACG GCCAATGCGA ATGGCTAGGG 
CGGATGCATG GAAGCATGGC TCCGCAAAAC CAGTTCACAG CCATGAGAAA CGCCTCAGGT 
CAAGCCAACC TTCACCAATC AGGCTTGATG ACGTCACATC ACAATGGCCG CCCTGCCACG 
CTCTCTCAAA TGATGAACTA TCAAAGCATG CAGAACACAC ATCTAATGCA GCAGATGCAG 
CAGAGCATGC AGCCGCGGCC CCAGCAGACC GGCGTCCAGC TGCAGAGCCA GAGCCAGAAC 
TTCATCGGGG GTGATCTGGG TGGACCGGAG CTCCAGCAGA GCGCAGGGAA CAGCATGTCC 
ATCCATACTA TAATCCCCCA GGAGACCCAG CTGCTCAACC CGTCCTCTCT GGGGTCCAGC 
ATGGCGGGCA CGCAGTTCCT GACGCCACCT TCCCAGCACA GTTACACTCC CGCATTGGAC 
GCCAACACTC CCAACCACCA ACTTCAAGTG CCTGACCACC ATCCTTTCCT CACGCCATCT 
CCCGGTTCTC CAGACCAGTG GTCCAGCTCG TCACCCAATT CCAACATGTC CGATTGGTCG 
GAGGGTATTT CCAGTCCTCC CACCAGCATG CAGTCACAAA TCGGACACAT GCCCGAGCAA 
TTCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hemodynamic-mediated endocardial signaling controls in vivo myocardial reprogramming.
eLife8(2019 06)
Gálvez-Santisteban M,Chen D,Zhang R,Serrano R,Nguyen C,Zhao L,Nerb L,Masutani EM,Vermot J,Burns CG,Burns CE,Del Álamo JC,Chi NC

EGFR is required for Wnt9a-Fzd9b signalling specificity in haematopoietic stem cells.
Nature cell biology21(6)721-730(2019 06)
Grainger S,Nguyen N,Richter J,Setayesh J,Lonquich B,Oon CH,Wozniak JM,Barahona R,Kamei CN,Houston J,Carrillo-Terrazas M,Drummond IA,Gonzalez D,Willert K,Traver D

Identification of a 42-bp heart-specific enhancer of the notch1b gene in zebrafish embryos.
Developmental dynamics : an official publication of the American Association of Anatomists248(6)426-436(2019 06)
Wang F,Yang Q,Wu F,Zhang Y,Sun S,Wang X,Gui Y,Li Q

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Cell reports27(5)1567-1578.e5(2019 04)
Xue Y,Liu D,Cui G,Ding Y,Ai D,Gao S,Zhang Y,Suo S,Wang X,Lv P,Zhou C,Li Y,Chen X,Peng G,Jing N,Han JJ,Liu F

Leptin-a mediates transcription of genes that participate in central endocrine and phosphatidylinositol signaling pathways in 72-hour embryonic zebrafish (
PeerJ7e6848(2019)
Tuttle M,Dalman MR,Liu Q,Londraville RL

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notch1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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