Hprt cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hprt
Entrez Gene ID	15452
Full Name	hypoxanthine guanine phosphoribosyl transferase
Synonyms	C81579,HPGRT,Hprt1
General protein information	Preferred Names hypoxanthine guanine phosphoribosyl transferase Names hypoxanthine-guanine phosphoribosyltransferase HGPRT HGPRTase HPRT B hypoxanthine guanine phosphoribosyl transferase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X A5\|X 29.31 cM
Summary	The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013556.2	NP_038584.2	hypoxanthine-guanine phosphoribosyltransferase

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	hprt1	NP_989312.1
Macaca mulatta (Rhesus monkey)	LOC709186	XP_001097691.1
Canis lupus familiaris (dog)	HPRT1	NP_001003357.1
Bos taurus (cattle)	LOC510369	XP_002686486.1
Mus musculus (house mouse)	Hprt	NP_038584.2
Gallus gallus (chicken)	HPRT1	NP_990179.1
Caenorhabditis elegans (roundworm)	CELE_Y105E8B.5	NP_493545.1
Homo sapiens (human)	HPRT1	NP_000185.1
Pan troglodytes (chimpanzee)	HPRT1	NP_001104287.1
Rattus norvegicus (Norway rat)	Hprt1	NP_036715.1
Danio rerio (zebrafish)	hprt1	NP_998151.1

Gene Ontology
Bibliography

Related articles in PubMed

EHMT2 and SETDB1 protect the maternal pronucleus from 5mC oxidation.
Zeng TB, Han L, Pierce N, Pfeifer GP, Szabó PE
Proceedings of the National Academy of Sciences of the United States of America116(22)10834-10841(2019 May)

CD34 defines melanocyte stem cell subpopulations with distinct regenerative properties.
Joshi SS, Tandukar B, Pan L, Huang JM, Livak F, Smith BJ, Hodges T, Mahurkar AA, Hornyak TJ
PLoS genetics15(4)e1008034(2019 04)

Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain, Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele.
Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
Genetics211(4)1155-1177(2019 04)

The Role of the Voltage-Gated Potassium Channel Proteins Kv8.2 and Kv2.1 in Vision and Retinal Disease: Insights from the Study of Mouse Gene Knock-Out Mutations.
Hart NS, Mountford JK, Voigt V, Fuller-Carter P, Barth M, Nerbonne JM, Hunt DM, Carvalho LS
eNeuro6(1)(2019 Jan-Feb)

Glandular defects in the mouse uterus with sustained activation of TGF-beta signaling is associated with altered differentiation of endometrial stromal cells and formation of stromal compartment.
Ni N, Gao Y, Fang X, Melgar M, Vincent DF, Lydon JP, Bartholin L, Li Q
PloS one13(12)e0209417(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hprt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hprt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21675	NM_013556.2 Latest version!	Mus musculus hypoxanthine guanine phosphoribosyl transferase (Hprt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hprt ( NM_013556.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038584.2 Mus musculus hypoxanthine guanine phosphoribosyl transferase (Hprt), mRNA.

Price & Availability↑

CloneID	OMu21675
Clone ID Related Accession (Same CDS sequence)	NM_013556.2
Accession Version	NM_013556.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Mus musculus(house mouse)
Product	hypoxanthine-guanine phosphoribosyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY285280.1, AK146626.1 and AK002286.1. On May 16, 2006 this sequence version replaced NM_013556.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146626.1, SRR1660815.313319.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGACCC GCAGTCCCAG CGTCGTGATT AGCGATGATG AACCAGGTTA TGACCTAGAT 
TTGTTTTGTA TACCTAATCA TTATGCCGAG GATTTGGAAA AAGTGTTTAT TCCTCATGGA 
CTGATTATGG ACAGGACTGA AAGACTTGCT CGAGATGTCA TGAAGGAGAT GGGAGGCCAT 
CACATTGTGG CCCTCTGTGT GCTCAAGGGG GGCTATAAGT TCTTTGCTGA CCTGCTGGAT 
TACATTAAAG CACTGAATAG AAATAGTGAT AGATCCATTC CTATGACTGT AGATTTTATC 
AGACTGAAGA GCTACTGTAA TGATCAGTCA ACGGGGGACA TAAAAGTTAT TGGTGGAGAT 
GATCTCTCAA CTTTAACTGG AAAGAATGTC TTGATTGTTG AAGATATAAT TGACACTGGT 
AAAACAATGC AAACTTTGCT TTCCCTGGTT AAGCAGTACA GCCCCAAAAT GGTTAAGGTT 
GCAAGCTTGC TGGTGAAAAG GACCTCTCGA AGTGTTGGAT ACAGGCCAGA CTTTGTTGGA 
TTTGAAATTC CAGACAAGTT TGTTGTTGGA TATGCCCTTG ACTATAATGA GTACTTCAGG 
GATTTGAATC ACGTTTGTGT CATTAGTGAA ACTGGAAAAG CCAAATACAA AGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038584.2
CDS	147..803
Translation	MPTRSPSVVISDDEPGYDLDLFCIPNHYAEDLEKVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVKQYSPKMVKVASLLVKRTSRSVGYRPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA

Target ORF information:

RefSeq Version	NM_013556.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypoxanthine guanine phosphoribosyl transferase (Hprt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013556.2

ORF Insert Sequence:

ATGCCGACCC GCAGTCCCAG CGTCGTGATT AGCGATGATG AACCAGGTTA TGACCTAGAT 
TTGTTTTGTA TACCTAATCA TTATGCCGAG GATTTGGAAA AAGTGTTTAT TCCTCATGGA 
CTGATTATGG ACAGGACTGA AAGACTTGCT CGAGATGTCA TGAAGGAGAT GGGAGGCCAT 
CACATTGTGG CCCTCTGTGT GCTCAAGGGG GGCTATAAGT TCTTTGCTGA CCTGCTGGAT 
TACATTAAAG CACTGAATAG AAATAGTGAT AGATCCATTC CTATGACTGT AGATTTTATC 
AGACTGAAGA GCTACTGTAA TGATCAGTCA ACGGGGGACA TAAAAGTTAT TGGTGGAGAT 
GATCTCTCAA CTTTAACTGG AAAGAATGTC TTGATTGTTG AAGATATAAT TGACACTGGT 
AAAACAATGC AAACTTTGCT TTCCCTGGTT AAGCAGTACA GCCCCAAAAT GGTTAAGGTT 
GCAAGCTTGC TGGTGAAAAG GACCTCTCGA AGTGTTGGAT ACAGGCCAGA CTTTGTTGGA 
TTTGAAATTC CAGACAAGTT TGTTGTTGGA TATGCCCTTG ACTATAATGA GTACTTCAGG 
GATTTGAATC ACGTTTGTGT CATTAGTGAA ACTGGAAAAG CCAAATACAA AGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EHMT2 and SETDB1 protect the maternal pronucleus from 5mC oxidation.
Proceedings of the National Academy of Sciences of the United States of America116(22)10834-10841(2019 May)
Zeng TB,Han L,Pierce N,Pfeifer GP,Szabó PE

CD34 defines melanocyte stem cell subpopulations with distinct regenerative properties.
PLoS genetics15(4)e1008034(2019 04)
Joshi SS,Tandukar B,Pan L,Huang JM,Livak F,Smith BJ,Hodges T,Mahurkar AA,Hornyak TJ

Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain, Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele.
Genetics211(4)1155-1177(2019 04)
Korecki AJ,Hickmott JW,Lam SL,Dreolini L,Mathelier A,Baker O,Kuehne C,Bonaguro RJ,Smith J,Tan CV,Zhou M,Goldowitz D,Deussing JM,Stewart AF,Wasserman WW,Holt RA,Simpson EM

The Role of the Voltage-Gated Potassium Channel Proteins Kv8.2 and Kv2.1 in Vision and Retinal Disease: Insights from the Study of Mouse Gene Knock-Out Mutations.
eNeuro6(1)(2019 Jan-Feb)
Hart NS,Mountford JK,Voigt V,Fuller-Carter P,Barth M,Nerbonne JM,Hunt DM,Carvalho LS

Glandular defects in the mouse uterus with sustained activation of TGF-beta signaling is associated with altered differentiation of endometrial stromal cells and formation of stromal compartment.
PloS one13(12)e0209417(2018)
Ni N,Gao Y,Fang X,Melgar M,Vincent DF,Lydon JP,Bartholin L,Li Q

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Hprt cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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