Sparcl1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sparcl1
Entrez Gene ID	13602
Full Name	SPARC-like 1
Synonyms	Ecm2,Sc1,hevin,mast9
General protein information	Preferred Names SPARC-like 1 Names SPARC-like protein 1 SPARC-like 1 (mast9, hevin) extracellular matrix protein 2 matrix glycoprotein Sc1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 E5\|5 50.55 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017320656.1	XP_017176145.1	SPARC-like protein 1 isoform X1
XM_006534768.1	XP_006534831.1	SPARC-like protein 1 isoform X1
NM_010097.4	NP_034227.3	SPARC-like protein 1 precursor
NM_001359014.1	NP_001345943.1	SPARC-like protein 1 precursor
NM_001359016.1	NP_001345945.1	SPARC-like protein 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPARCL1	NP_004675.3
Macaca mulatta (Rhesus monkey)	SPARCL1	XP_001098328.2
Mus musculus (house mouse)	Sparcl1	NP_034227.3
Drosophila melanogaster (fruit fly)	BM-40-SPARC	NP_001262998.1
Caenorhabditis elegans (roundworm)	ost-1	NP_500039.1
Pan troglodytes (chimpanzee)	SPARCL1	NP_001092036.1
Bos taurus (cattle)	SPARCL1	NP_001029474.1
Canis lupus familiaris (dog)	SPARCL1	XP_005639220.1
Rattus norvegicus (Norway rat)	Sparcl1	NP_037078.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000305	XP_001237311.3

Gene Ontology
Bibliography

Related articles in PubMed

SPARCL1 suppresses osteosarcoma metastasis and recruits macrophages by activation of canonical WNT/β-catenin signaling through stabilization of the WNT-receptor complex.
Zhao SJ, Jiang YQ, Xu NW, Li Q, Zhang Q, Wang SY, Li J, Wang YH, Zhang YL, Jiang SH, Wang YJ, Huang YJ, Zhang XX, Tian GA, Zhang CC, Lv YY, Dai M, Liu F, Zhang R, Zhou D, Zhang ZG
Oncogene37(8)1049-1061(2018 02)

Altered Anterior Segment Biometric Parameters in Mice Deficient in SPARC.
Ho H, Htoon HM, Yam GH, Toh LZ, Lwin NC, Chu S, Lee YS, Wong TT, Seet LF
Investigative ophthalmology & visual science58(1)386-393(2017 01)

Spatiotemporal differences in otoconial gene expression.
Xu Y, Zhang Y, Lundberg YW
Genesis (New York, N.Y. : 2000)54(12)613-625(2016 Dec)

Matricellular protein SPARCL1 regulates tumor microenvironment-dependent endothelial cell heterogeneity in colorectal carcinoma.
Naschberger E, Liebl A, Schellerer VS, Schütz M, Britzen-Laurent N, Kölbel P, Schaal U, Haep L, Regensburger D, Wittmann T, Klein-Hitpass L, Rau TT, Dietel B, Méniel VS, Clarke AR, Merkel S, Croner RS, Hohenberger W, Stürzl M
The Journal of clinical investigation126(11)4187-4204(2016 11)

Regulation of adipogenesis by paracrine factors from adipose stromal-vascular fraction - a link to fat depot-specific differences.
Meissburger B, Perdikari A, Moest H, Müller S, Geiger M, Wolfrum C
Biochimica et biophysica acta1861(9 Pt A)1121-1131(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sparcl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sparcl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05524	NM_001359014.1 Latest version!	Mus musculus SPARC-like 1 (Sparcl1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu05524	NM_001359016.1 Latest version!	Mus musculus SPARC-like 1 (Sparcl1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu05524	NM_010097.4 Latest version!	Mus musculus SPARC-like 1 (Sparcl1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu05524	XM_017320656.1 Latest version!	Mus musculus SPARC-like 1 (Sparcl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu05524	XM_006534768.1 Latest version!	Mus musculus SPARC-like 1 (Sparcl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sparcl1 ( NM_010097.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034227.3 Mus musculus SPARC-like 1 (Sparcl1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu05524
Clone ID Related Accession (Same CDS sequence)	XM_017320656.1 , XM_006534768.1 , NM_010097.4 , NM_001359014.1 , NM_001359016.1
Accession Version	NM_010097.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572796800000
Organism	Mus musculus(house mouse)
Product	SPARC-like protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY277497.1, AK134608.1, AK158725.1 and CD742522.1. On Apr 22, 2009 this sequence version replaced NM_010097.3. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003759.1, SRR1660821.159286.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGCTG TGCTTCTCCT CCTGTGCGCC TTGGGAACCG CTGTGGCAAT CCCGACAAGT 
ACAAGGTTTC TCTCTGACCA CTCCAACCCA ACTACTGCAA CACTGGTGAC ACCGGAAGAC 
GCTACAGTCC CCATTGCCGG GGTTGAAGCT ACAGCAGACA TAGAAAACCA TCCCAGTGAC 
AAGGCTGAAA AACCTTCAGC ACTTAATTCA GAAGAGGAAA CTCATGAACA GTCAACAGAG 
CAGGACAAAA CCTACAGCTT CGAGGTGGAC CTGAAGGATG AGGAGGATGG AGATGGGGAT 
TTAAGTGTAG ATCCAACGGA AGGAACACTA ACACTGGATC TACAAGAAGG TACAAGTGAG 
CCTCAACAGA AAAGTCTCCC GGAGAACGGC GATTTCCCCG CGACCGTGTC CACTTCCTAT 
GTGGATCCTA ACCAACGCGC AAACATCACA AAGGGAAAGG AGAGTCAGGA GCAACCTGTA 
AGTGACTCAC ACCAGCAACC GAATGAAAGC AGCAAGCAAA CCCAAGACTT AAAGGCTGAA 
GAAAGCCAGA CACAAGATCC AGACATTCCC AATGAAGAAG AGGAAGAAGA AGAGGACGAA 
GAAGAGGAAG AAGAGGAAGA GCCGGAAGAC ATTGGTGCCC CCAGTGATAA CCAAGAGGAG 
GGAAAAGAAC CTCTGGAGGA GCAGCCTACC AGCAAGTGGG AAGGAAACAG AGAGCAATCT 
GATGACACCT TAGAAGAGTC CAGTCAGCCC ACTCAGATAA GCAAGACAGA GAAGCATCAA 
TCTGAGCAAG GAAACCAAGG GCAGGAGAGT GACTCTGAGG CAGAAGGAGA GGACAAGGCT 
GCAGGCAGCA AGGAACACAT TCCACATACA GAGCAGCAGG ACCAAGAAGG GAAAGCTGGC 
CTTGAAGCTA TTGGCAACCA GAAGGACACT GATGAGAAGG CCGTTTCCAC AGAACCTACC 
GATGCTGCCG TGGTGCCTAG GAGTCACGGA GGAGCTGGTG ATAACGGGGG CGGGGATGAC 
TCTAAGCATG GTGCAGGCGA TGACTACTTC ATCCCCAGCC AGGAATTCCT AGAGGCCGAA 
AGGATGCATT CCCTCTCCTA TTACCTCAAA TATGGCGGCG GCGAGGAGAC AACGACTGGC 
GAGAGTGAGA ACCGGAGGGA GGCTGCAGAC AACCAAGAGG CCAAGAAAGC TGAGAGCTCA 
CCAAATGCTG AACCTTCAGA TGAGGGCAAC TCAAGGGAGC ACAGTGCTGG TTCTTGCACG 
AACTTCCAAT GTAAAAGGGG ACACATTTGC AAAACCGATC CACAAGGGAA ACCTCACTGT 
GTTTGCCAAG ATCCAGAGAC TTGTCCCCCT GCAAAAATCC TAGATCAGGC TTGTGGCACT 
GACAACCAAA CCTACGCCAG CTCCTGTCAC CTGTTTGCTA CCAAGTGCAG GCTGGAGGGG 
ACCAAAAAGG GACACCAACT GCAGCTGGAT TACTTCGGAG CTTGCAAATC TATTCCTGCT 
TGTACGGACT TTGAAGTGGC TCAGTTTCCC CTGCGGATGA GAGACTGGCT CAAAAACATC 
CTCATGCAGC TTTATGAACC AAATCCCAAA CATGGCGGCT ATCTCAATGA AAAGCAAAGA 
AGCAAAGTCA AAAAAATTTA CCTGGATGAG AAGAGACTCT TGGCTGGAGA CCATCCCATT 
GAACTTCTCT TGAGGGACTT TAAGAAAAAC TACCACATGT ATGTGTATCC TGTGCACTGG 
CAGTTTAATG AACTGGACCA GCATCCTGCA GACAGGATCT TGACACACTC TGAACTTGCT 
CCTCTGCGAG CTTCCCTGGT GCCCATGGAA CACTGCATAA CTCGCTTCTT TGAGGAGTGT 
GACCCCAACA AGGATAAGCA CATCACCTTG AAGGAATGGG GCCACTGCTT TGGAATTAAA 
GAGGAGGATA TAGATGAAAA CCTCCTCTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034227.3
CDS	724..2676
Translation	MKAVLLLLCALGTAVAIPTSTRFLSDHSNPTTATLVTPEDATVPIAGVEATADIENHPSDKAEKPSALNSEEETHEQSTEQDKTYSFEVDLKDEEDGDGDLSVDPTEGTLTLDLQEGTSEPQQKSLPENGDFPATVSTSYVDPNQRANITKGKESQEQPVSDSHQQPNESSKQTQDLKAEESQTQDPDIPNEEEEEEEDEEEEEEEEPEDIGAPSDNQEEGKEPLEEQPTSKWEGNREQSDDTLEESSQPTQISKTEKHQSEQGNQGQESDSEAEGEDKAAGSKEHIPHTEQQDQEGKAGLEAIGNQKDTDEKAVSTEPTDAAVVPRSHGGAGDNGGGDDSKHGAGDDYFIPSQEFLEAERMHSLSYYLKYGGGEETTTGESENRREAADNQEAKKAESSPNAEPSDEGNSREHSAGSCTNFQCKRGHICKTDPQGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPACTDFEVAQFPLRMRDWLKNILMQLYEPNPKHGGYLNEKQRSKVKKIYLDEKRLLAGDHPIELLLRDFKKNYHMYVYPVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

Target ORF information:

RefSeq Version	NM_010097.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPARC-like 1 (Sparcl1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010097.4

ORF Insert Sequence:

ATGAAGGCTG TGCTTCTCCT CCTGTGCGCC TTGGGAACCG CTGTGGCAAT CCCGACAAGT 
ACAAGGTTTC TCTCTGACCA CTCCAACCCA ACTACTGCAA CACTGGTGAC ACCGGAAGAC 
GCTACAGTCC CCATTGCCGG GGTTGAAGCT ACAGCAGACA TAGAAAACCA TCCCAGTGAC 
AAGGCTGAAA AACCTTCAGC ACTTAATTCA GAAGAGGAAA CTCATGAACA GTCAACAGAG 
CAGGACAAAA CCTACAGCTT CGAGGTGGAC CTGAAGGATG AGGAGGATGG AGATGGGGAT 
TTAAGTGTAG ATCCAACGGA AGGAACACTA ACACTGGATC TACAAGAAGG TACAAGTGAG 
CCTCAACAGA AAAGTCTCCC GGAGAACGGC GATTTCCCCG CGACCGTGTC CACTTCCTAT 
GTGGATCCTA ACCAACGCGC AAACATCACA AAGGGAAAGG AGAGTCAGGA GCAACCTGTA 
AGTGACTCAC ACCAGCAACC GAATGAAAGC AGCAAGCAAA CCCAAGACTT AAAGGCTGAA 
GAAAGCCAGA CACAAGATCC AGACATTCCC AATGAAGAAG AGGAAGAAGA AGAGGACGAA 
GAAGAGGAAG AAGAGGAAGA GCCGGAAGAC ATTGGTGCCC CCAGTGATAA CCAAGAGGAG 
GGAAAAGAAC CTCTGGAGGA GCAGCCTACC AGCAAGTGGG AAGGAAACAG AGAGCAATCT 
GATGACACCT TAGAAGAGTC CAGTCAGCCC ACTCAGATAA GCAAGACAGA GAAGCATCAA 
TCTGAGCAAG GAAACCAAGG GCAGGAGAGT GACTCTGAGG CAGAAGGAGA GGACAAGGCT 
GCAGGCAGCA AGGAACACAT TCCACATACA GAGCAGCAGG ACCAAGAAGG GAAAGCTGGC 
CTTGAAGCTA TTGGCAACCA GAAGGACACT GATGAGAAGG CCGTTTCCAC AGAACCTACC 
GATGCTGCCG TGGTGCCTAG GAGTCACGGA GGAGCTGGTG ATAACGGGGG CGGGGATGAC 
TCTAAGCATG GTGCAGGCGA TGACTACTTC ATCCCCAGCC AGGAATTCCT AGAGGCCGAA 
AGGATGCATT CCCTCTCCTA TTACCTCAAA TATGGCGGCG GCGAGGAGAC AACGACTGGC 
GAGAGTGAGA ACCGGAGGGA GGCTGCAGAC AACCAAGAGG CCAAGAAAGC TGAGAGCTCA 
CCAAATGCTG AACCTTCAGA TGAGGGCAAC TCAAGGGAGC ACAGTGCTGG TTCTTGCACG 
AACTTCCAAT GTAAAAGGGG ACACATTTGC AAAACCGATC CACAAGGGAA ACCTCACTGT 
GTTTGCCAAG ATCCAGAGAC TTGTCCCCCT GCAAAAATCC TAGATCAGGC TTGTGGCACT 
GACAACCAAA CCTACGCCAG CTCCTGTCAC CTGTTTGCTA CCAAGTGCAG GCTGGAGGGG 
ACCAAAAAGG GACACCAACT GCAGCTGGAT TACTTCGGAG CTTGCAAATC TATTCCTGCT 
TGTACGGACT TTGAAGTGGC TCAGTTTCCC CTGCGGATGA GAGACTGGCT CAAAAACATC 
CTCATGCAGC TTTATGAACC AAATCCCAAA CATGGCGGCT ATCTCAATGA AAAGCAAAGA 
AGCAAAGTCA AAAAAATTTA CCTGGATGAG AAGAGACTCT TGGCTGGAGA CCATCCCATT 
GAACTTCTCT TGAGGGACTT TAAGAAAAAC TACCACATGT ATGTGTATCC TGTGCACTGG 
CAGTTTAATG AACTGGACCA GCATCCTGCA GACAGGATCT TGACACACTC TGAACTTGCT 
CCTCTGCGAG CTTCCCTGGT GCCCATGGAA CACTGCATAA CTCGCTTCTT TGAGGAGTGT 
GACCCCAACA AGGATAAGCA CATCACCTTG AAGGAATGGG GCCACTGCTT TGGAATTAAA 
GAGGAGGATA TAGATGAAAA CCTCCTCTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sparcl1 ( NM_001359014.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001345943.1 Mus musculus SPARC-like 1 (Sparcl1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05524
Clone ID Related Accession (Same CDS sequence)	XM_017320656.1 , XM_006534768.1 , NM_010097.4 , NM_001359014.1 , NM_001359016.1
Accession Version	NM_001359014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572796800000
Organism	Mus musculus(house mouse)
Product	SPARC-like protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL714024.31. On Dec 16, 2017 this sequence version replaced XM_006534768.1. Transcript Variant: This variant (1) represents the longest transcript. All three variants encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.301764.1, SRR1660819.116555.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGCTG TGCTTCTCCT CCTGTGCGCC TTGGGAACCG CTGTGGCAAT CCCGACAAGT 
ACAAGGTTTC TCTCTGACCA CTCCAACCCA ACTACTGCAA CACTGGTGAC ACCGGAAGAC 
GCTACAGTCC CCATTGCCGG GGTTGAAGCT ACAGCAGACA TAGAAAACCA TCCCAGTGAC 
AAGGCTGAAA AACCTTCAGC ACTTAATTCA GAAGAGGAAA CTCATGAACA GTCAACAGAG 
CAGGACAAAA CCTACAGCTT CGAGGTGGAC CTGAAGGATG AGGAGGATGG AGATGGGGAT 
TTAAGTGTAG ATCCAACGGA AGGAACACTA ACACTGGATC TACAAGAAGG TACAAGTGAG 
CCTCAACAGA AAAGTCTCCC GGAGAACGGC GATTTCCCCG CGACCGTGTC CACTTCCTAT 
GTGGATCCTA ACCAACGCGC AAACATCACA AAGGGAAAGG AGAGTCAGGA GCAACCTGTA 
AGTGACTCAC ACCAGCAACC GAATGAAAGC AGCAAGCAAA CCCAAGACTT AAAGGCTGAA 
GAAAGCCAGA CACAAGATCC AGACATTCCC AATGAAGAAG AGGAAGAAGA AGAGGACGAA 
GAAGAGGAAG AAGAGGAAGA GCCGGAAGAC ATTGGTGCCC CCAGTGATAA CCAAGAGGAG 
GGAAAAGAAC CTCTGGAGGA GCAGCCTACC AGCAAGTGGG AAGGAAACAG AGAGCAATCT 
GATGACACCT TAGAAGAGTC CAGTCAGCCC ACTCAGATAA GCAAGACAGA GAAGCATCAA 
TCTGAGCAAG GAAACCAAGG GCAGGAGAGT GACTCTGAGG CAGAAGGAGA GGACAAGGCT 
GCAGGCAGCA AGGAACACAT TCCACATACA GAGCAGCAGG ACCAAGAAGG GAAAGCTGGC 
CTTGAAGCTA TTGGCAACCA GAAGGACACT GATGAGAAGG CCGTTTCCAC AGAACCTACC 
GATGCTGCCG TGGTGCCTAG GAGTCACGGA GGAGCTGGTG ATAACGGGGG CGGGGATGAC 
TCTAAGCATG GTGCAGGCGA TGACTACTTC ATCCCCAGCC AGGAATTCCT AGAGGCCGAA 
AGGATGCATT CCCTCTCCTA TTACCTCAAA TATGGCGGCG GCGAGGAGAC AACGACTGGC 
GAGAGTGAGA ACCGGAGGGA GGCTGCAGAC AACCAAGAGG CCAAGAAAGC TGAGAGCTCA 
CCAAATGCTG AACCTTCAGA TGAGGGCAAC TCAAGGGAGC ACAGTGCTGG TTCTTGCACG 
AACTTCCAAT GTAAAAGGGG ACACATTTGC AAAACCGATC CACAAGGGAA ACCTCACTGT 
GTTTGCCAAG ATCCAGAGAC TTGTCCCCCT GCAAAAATCC TAGATCAGGC TTGTGGCACT 
GACAACCAAA CCTACGCCAG CTCCTGTCAC CTGTTTGCTA CCAAGTGCAG GCTGGAGGGG 
ACCAAAAAGG GACACCAACT GCAGCTGGAT TACTTCGGAG CTTGCAAATC TATTCCTGCT 
TGTACGGACT TTGAAGTGGC TCAGTTTCCC CTGCGGATGA GAGACTGGCT CAAAAACATC 
CTCATGCAGC TTTATGAACC AAATCCCAAA CATGGCGGCT ATCTCAATGA AAAGCAAAGA 
AGCAAAGTCA AAAAAATTTA CCTGGATGAG AAGAGACTCT TGGCTGGAGA CCATCCCATT 
GAACTTCTCT TGAGGGACTT TAAGAAAAAC TACCACATGT ATGTGTATCC TGTGCACTGG 
CAGTTTAATG AACTGGACCA GCATCCTGCA GACAGGATCT TGACACACTC TGAACTTGCT 
CCTCTGCGAG CTTCCCTGGT GCCCATGGAA CACTGCATAA CTCGCTTCTT TGAGGAGTGT 
GACCCCAACA AGGATAAGCA CATCACCTTG AAGGAATGGG GCCACTGCTT TGGAATTAAA 
GAGGAGGATA TAGATGAAAA CCTCCTCTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345943.1
CDS	902..2854
Translation	MKAVLLLLCALGTAVAIPTSTRFLSDHSNPTTATLVTPEDATVPIAGVEATADIENHPSDKAEKPSALNSEEETHEQSTEQDKTYSFEVDLKDEEDGDGDLSVDPTEGTLTLDLQEGTSEPQQKSLPENGDFPATVSTSYVDPNQRANITKGKESQEQPVSDSHQQPNESSKQTQDLKAEESQTQDPDIPNEEEEEEEDEEEEEEEEPEDIGAPSDNQEEGKEPLEEQPTSKWEGNREQSDDTLEESSQPTQISKTEKHQSEQGNQGQESDSEAEGEDKAAGSKEHIPHTEQQDQEGKAGLEAIGNQKDTDEKAVSTEPTDAAVVPRSHGGAGDNGGGDDSKHGAGDDYFIPSQEFLEAERMHSLSYYLKYGGGEETTTGESENRREAADNQEAKKAESSPNAEPSDEGNSREHSAGSCTNFQCKRGHICKTDPQGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPACTDFEVAQFPLRMRDWLKNILMQLYEPNPKHGGYLNEKQRSKVKKIYLDEKRLLAGDHPIELLLRDFKKNYHMYVYPVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

Target ORF information:

RefSeq Version	NM_001359014.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPARC-like 1 (Sparcl1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359014.1

ORF Insert Sequence:

ATGAAGGCTG TGCTTCTCCT CCTGTGCGCC TTGGGAACCG CTGTGGCAAT CCCGACAAGT 
ACAAGGTTTC TCTCTGACCA CTCCAACCCA ACTACTGCAA CACTGGTGAC ACCGGAAGAC 
GCTACAGTCC CCATTGCCGG GGTTGAAGCT ACAGCAGACA TAGAAAACCA TCCCAGTGAC 
AAGGCTGAAA AACCTTCAGC ACTTAATTCA GAAGAGGAAA CTCATGAACA GTCAACAGAG 
CAGGACAAAA CCTACAGCTT CGAGGTGGAC CTGAAGGATG AGGAGGATGG AGATGGGGAT 
TTAAGTGTAG ATCCAACGGA AGGAACACTA ACACTGGATC TACAAGAAGG TACAAGTGAG 
CCTCAACAGA AAAGTCTCCC GGAGAACGGC GATTTCCCCG CGACCGTGTC CACTTCCTAT 
GTGGATCCTA ACCAACGCGC AAACATCACA AAGGGAAAGG AGAGTCAGGA GCAACCTGTA 
AGTGACTCAC ACCAGCAACC GAATGAAAGC AGCAAGCAAA CCCAAGACTT AAAGGCTGAA 
GAAAGCCAGA CACAAGATCC AGACATTCCC AATGAAGAAG AGGAAGAAGA AGAGGACGAA 
GAAGAGGAAG AAGAGGAAGA GCCGGAAGAC ATTGGTGCCC CCAGTGATAA CCAAGAGGAG 
GGAAAAGAAC CTCTGGAGGA GCAGCCTACC AGCAAGTGGG AAGGAAACAG AGAGCAATCT 
GATGACACCT TAGAAGAGTC CAGTCAGCCC ACTCAGATAA GCAAGACAGA GAAGCATCAA 
TCTGAGCAAG GAAACCAAGG GCAGGAGAGT GACTCTGAGG CAGAAGGAGA GGACAAGGCT 
GCAGGCAGCA AGGAACACAT TCCACATACA GAGCAGCAGG ACCAAGAAGG GAAAGCTGGC 
CTTGAAGCTA TTGGCAACCA GAAGGACACT GATGAGAAGG CCGTTTCCAC AGAACCTACC 
GATGCTGCCG TGGTGCCTAG GAGTCACGGA GGAGCTGGTG ATAACGGGGG CGGGGATGAC 
TCTAAGCATG GTGCAGGCGA TGACTACTTC ATCCCCAGCC AGGAATTCCT AGAGGCCGAA 
AGGATGCATT CCCTCTCCTA TTACCTCAAA TATGGCGGCG GCGAGGAGAC AACGACTGGC 
GAGAGTGAGA ACCGGAGGGA GGCTGCAGAC AACCAAGAGG CCAAGAAAGC TGAGAGCTCA 
CCAAATGCTG AACCTTCAGA TGAGGGCAAC TCAAGGGAGC ACAGTGCTGG TTCTTGCACG 
AACTTCCAAT GTAAAAGGGG ACACATTTGC AAAACCGATC CACAAGGGAA ACCTCACTGT 
GTTTGCCAAG ATCCAGAGAC TTGTCCCCCT GCAAAAATCC TAGATCAGGC TTGTGGCACT 
GACAACCAAA CCTACGCCAG CTCCTGTCAC CTGTTTGCTA CCAAGTGCAG GCTGGAGGGG 
ACCAAAAAGG GACACCAACT GCAGCTGGAT TACTTCGGAG CTTGCAAATC TATTCCTGCT 
TGTACGGACT TTGAAGTGGC TCAGTTTCCC CTGCGGATGA GAGACTGGCT CAAAAACATC 
CTCATGCAGC TTTATGAACC AAATCCCAAA CATGGCGGCT ATCTCAATGA AAAGCAAAGA 
AGCAAAGTCA AAAAAATTTA CCTGGATGAG AAGAGACTCT TGGCTGGAGA CCATCCCATT 
GAACTTCTCT TGAGGGACTT TAAGAAAAAC TACCACATGT ATGTGTATCC TGTGCACTGG 
CAGTTTAATG AACTGGACCA GCATCCTGCA GACAGGATCT TGACACACTC TGAACTTGCT 
CCTCTGCGAG CTTCCCTGGT GCCCATGGAA CACTGCATAA CTCGCTTCTT TGAGGAGTGT 
GACCCCAACA AGGATAAGCA CATCACCTTG AAGGAATGGG GCCACTGCTT TGGAATTAAA 
GAGGAGGATA TAGATGAAAA CCTCCTCTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sparcl1 ( NM_001359016.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001345945.1 Mus musculus SPARC-like 1 (Sparcl1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05524
Clone ID Related Accession (Same CDS sequence)	XM_017320656.1 , XM_006534768.1 , NM_010097.4 , NM_001359014.1 , NM_001359016.1
Accession Version	NM_001359016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572796800000
Organism	Mus musculus(house mouse)
Product	SPARC-like protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL714024.31. On Dec 16, 2017 this sequence version replaced XM_017320656.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.121179.1, SRR1660813.148749.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGCTG TGCTTCTCCT CCTGTGCGCC TTGGGAACCG CTGTGGCAAT CCCGACAAGT 
ACAAGGTTTC TCTCTGACCA CTCCAACCCA ACTACTGCAA CACTGGTGAC ACCGGAAGAC 
GCTACAGTCC CCATTGCCGG GGTTGAAGCT ACAGCAGACA TAGAAAACCA TCCCAGTGAC 
AAGGCTGAAA AACCTTCAGC ACTTAATTCA GAAGAGGAAA CTCATGAACA GTCAACAGAG 
CAGGACAAAA CCTACAGCTT CGAGGTGGAC CTGAAGGATG AGGAGGATGG AGATGGGGAT 
TTAAGTGTAG ATCCAACGGA AGGAACACTA ACACTGGATC TACAAGAAGG TACAAGTGAG 
CCTCAACAGA AAAGTCTCCC GGAGAACGGC GATTTCCCCG CGACCGTGTC CACTTCCTAT 
GTGGATCCTA ACCAACGCGC AAACATCACA AAGGGAAAGG AGAGTCAGGA GCAACCTGTA 
AGTGACTCAC ACCAGCAACC GAATGAAAGC AGCAAGCAAA CCCAAGACTT AAAGGCTGAA 
GAAAGCCAGA CACAAGATCC AGACATTCCC AATGAAGAAG AGGAAGAAGA AGAGGACGAA 
GAAGAGGAAG AAGAGGAAGA GCCGGAAGAC ATTGGTGCCC CCAGTGATAA CCAAGAGGAG 
GGAAAAGAAC CTCTGGAGGA GCAGCCTACC AGCAAGTGGG AAGGAAACAG AGAGCAATCT 
GATGACACCT TAGAAGAGTC CAGTCAGCCC ACTCAGATAA GCAAGACAGA GAAGCATCAA 
TCTGAGCAAG GAAACCAAGG GCAGGAGAGT GACTCTGAGG CAGAAGGAGA GGACAAGGCT 
GCAGGCAGCA AGGAACACAT TCCACATACA GAGCAGCAGG ACCAAGAAGG GAAAGCTGGC 
CTTGAAGCTA TTGGCAACCA GAAGGACACT GATGAGAAGG CCGTTTCCAC AGAACCTACC 
GATGCTGCCG TGGTGCCTAG GAGTCACGGA GGAGCTGGTG ATAACGGGGG CGGGGATGAC 
TCTAAGCATG GTGCAGGCGA TGACTACTTC ATCCCCAGCC AGGAATTCCT AGAGGCCGAA 
AGGATGCATT CCCTCTCCTA TTACCTCAAA TATGGCGGCG GCGAGGAGAC AACGACTGGC 
GAGAGTGAGA ACCGGAGGGA GGCTGCAGAC AACCAAGAGG CCAAGAAAGC TGAGAGCTCA 
CCAAATGCTG AACCTTCAGA TGAGGGCAAC TCAAGGGAGC ACAGTGCTGG TTCTTGCACG 
AACTTCCAAT GTAAAAGGGG ACACATTTGC AAAACCGATC CACAAGGGAA ACCTCACTGT 
GTTTGCCAAG ATCCAGAGAC TTGTCCCCCT GCAAAAATCC TAGATCAGGC TTGTGGCACT 
GACAACCAAA CCTACGCCAG CTCCTGTCAC CTGTTTGCTA CCAAGTGCAG GCTGGAGGGG 
ACCAAAAAGG GACACCAACT GCAGCTGGAT TACTTCGGAG CTTGCAAATC TATTCCTGCT 
TGTACGGACT TTGAAGTGGC TCAGTTTCCC CTGCGGATGA GAGACTGGCT CAAAAACATC 
CTCATGCAGC TTTATGAACC AAATCCCAAA CATGGCGGCT ATCTCAATGA AAAGCAAAGA 
AGCAAAGTCA AAAAAATTTA CCTGGATGAG AAGAGACTCT TGGCTGGAGA CCATCCCATT 
GAACTTCTCT TGAGGGACTT TAAGAAAAAC TACCACATGT ATGTGTATCC TGTGCACTGG 
CAGTTTAATG AACTGGACCA GCATCCTGCA GACAGGATCT TGACACACTC TGAACTTGCT 
CCTCTGCGAG CTTCCCTGGT GCCCATGGAA CACTGCATAA CTCGCTTCTT TGAGGAGTGT 
GACCCCAACA AGGATAAGCA CATCACCTTG AAGGAATGGG GCCACTGCTT TGGAATTAAA 
GAGGAGGATA TAGATGAAAA CCTCCTCTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345945.1
CDS	849..2801
Translation	MKAVLLLLCALGTAVAIPTSTRFLSDHSNPTTATLVTPEDATVPIAGVEATADIENHPSDKAEKPSALNSEEETHEQSTEQDKTYSFEVDLKDEEDGDGDLSVDPTEGTLTLDLQEGTSEPQQKSLPENGDFPATVSTSYVDPNQRANITKGKESQEQPVSDSHQQPNESSKQTQDLKAEESQTQDPDIPNEEEEEEEDEEEEEEEEPEDIGAPSDNQEEGKEPLEEQPTSKWEGNREQSDDTLEESSQPTQISKTEKHQSEQGNQGQESDSEAEGEDKAAGSKEHIPHTEQQDQEGKAGLEAIGNQKDTDEKAVSTEPTDAAVVPRSHGGAGDNGGGDDSKHGAGDDYFIPSQEFLEAERMHSLSYYLKYGGGEETTTGESENRREAADNQEAKKAESSPNAEPSDEGNSREHSAGSCTNFQCKRGHICKTDPQGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPACTDFEVAQFPLRMRDWLKNILMQLYEPNPKHGGYLNEKQRSKVKKIYLDEKRLLAGDHPIELLLRDFKKNYHMYVYPVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

Target ORF information:

RefSeq Version	NM_001359016.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPARC-like 1 (Sparcl1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359016.1

ORF Insert Sequence:

ATGAAGGCTG TGCTTCTCCT CCTGTGCGCC TTGGGAACCG CTGTGGCAAT CCCGACAAGT 
ACAAGGTTTC TCTCTGACCA CTCCAACCCA ACTACTGCAA CACTGGTGAC ACCGGAAGAC 
GCTACAGTCC CCATTGCCGG GGTTGAAGCT ACAGCAGACA TAGAAAACCA TCCCAGTGAC 
AAGGCTGAAA AACCTTCAGC ACTTAATTCA GAAGAGGAAA CTCATGAACA GTCAACAGAG 
CAGGACAAAA CCTACAGCTT CGAGGTGGAC CTGAAGGATG AGGAGGATGG AGATGGGGAT 
TTAAGTGTAG ATCCAACGGA AGGAACACTA ACACTGGATC TACAAGAAGG TACAAGTGAG 
CCTCAACAGA AAAGTCTCCC GGAGAACGGC GATTTCCCCG CGACCGTGTC CACTTCCTAT 
GTGGATCCTA ACCAACGCGC AAACATCACA AAGGGAAAGG AGAGTCAGGA GCAACCTGTA 
AGTGACTCAC ACCAGCAACC GAATGAAAGC AGCAAGCAAA CCCAAGACTT AAAGGCTGAA 
GAAAGCCAGA CACAAGATCC AGACATTCCC AATGAAGAAG AGGAAGAAGA AGAGGACGAA 
GAAGAGGAAG AAGAGGAAGA GCCGGAAGAC ATTGGTGCCC CCAGTGATAA CCAAGAGGAG 
GGAAAAGAAC CTCTGGAGGA GCAGCCTACC AGCAAGTGGG AAGGAAACAG AGAGCAATCT 
GATGACACCT TAGAAGAGTC CAGTCAGCCC ACTCAGATAA GCAAGACAGA GAAGCATCAA 
TCTGAGCAAG GAAACCAAGG GCAGGAGAGT GACTCTGAGG CAGAAGGAGA GGACAAGGCT 
GCAGGCAGCA AGGAACACAT TCCACATACA GAGCAGCAGG ACCAAGAAGG GAAAGCTGGC 
CTTGAAGCTA TTGGCAACCA GAAGGACACT GATGAGAAGG CCGTTTCCAC AGAACCTACC 
GATGCTGCCG TGGTGCCTAG GAGTCACGGA GGAGCTGGTG ATAACGGGGG CGGGGATGAC 
TCTAAGCATG GTGCAGGCGA TGACTACTTC ATCCCCAGCC AGGAATTCCT AGAGGCCGAA 
AGGATGCATT CCCTCTCCTA TTACCTCAAA TATGGCGGCG GCGAGGAGAC AACGACTGGC 
GAGAGTGAGA ACCGGAGGGA GGCTGCAGAC AACCAAGAGG CCAAGAAAGC TGAGAGCTCA 
CCAAATGCTG AACCTTCAGA TGAGGGCAAC TCAAGGGAGC ACAGTGCTGG TTCTTGCACG 
AACTTCCAAT GTAAAAGGGG ACACATTTGC AAAACCGATC CACAAGGGAA ACCTCACTGT 
GTTTGCCAAG ATCCAGAGAC TTGTCCCCCT GCAAAAATCC TAGATCAGGC TTGTGGCACT 
GACAACCAAA CCTACGCCAG CTCCTGTCAC CTGTTTGCTA CCAAGTGCAG GCTGGAGGGG 
ACCAAAAAGG GACACCAACT GCAGCTGGAT TACTTCGGAG CTTGCAAATC TATTCCTGCT 
TGTACGGACT TTGAAGTGGC TCAGTTTCCC CTGCGGATGA GAGACTGGCT CAAAAACATC 
CTCATGCAGC TTTATGAACC AAATCCCAAA CATGGCGGCT ATCTCAATGA AAAGCAAAGA 
AGCAAAGTCA AAAAAATTTA CCTGGATGAG AAGAGACTCT TGGCTGGAGA CCATCCCATT 
GAACTTCTCT TGAGGGACTT TAAGAAAAAC TACCACATGT ATGTGTATCC TGTGCACTGG 
CAGTTTAATG AACTGGACCA GCATCCTGCA GACAGGATCT TGACACACTC TGAACTTGCT 
CCTCTGCGAG CTTCCCTGGT GCCCATGGAA CACTGCATAA CTCGCTTCTT TGAGGAGTGT 
GACCCCAACA AGGATAAGCA CATCACCTTG AAGGAATGGG GCCACTGCTT TGGAATTAAA 
GAGGAGGATA TAGATGAAAA CCTCCTCTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sparcl1 ( XM_017320656.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176145.1 Mus musculus SPARC-like 1 (Sparcl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu05524
Clone ID Related Accession (Same CDS sequence)	XM_017320656.1 , XM_006534768.1 , NM_010097.4 , NM_001359014.1 , NM_001359016.1
Accession Version	XM_017320656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(House mouse)
Product	SPARC-like protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTG TGCTTCTCCT CCTGTGCGCC TTGGGAACCG CTGTGGCAAT CCCGACAAGT 
ACAAGGTTTC TCTCTGACCA CTCCAACCCA ACTACTGCAA CACTGGTGAC ACCGGAAGAC 
GCTACAGTCC CCATTGCCGG GGTTGAAGCT ACAGCAGACA TAGAAAACCA TCCCAGTGAC 
AAGGCTGAAA AACCTTCAGC ACTTAATTCA GAAGAGGAAA CTCATGAACA GTCAACAGAG 
CAGGACAAAA CCTACAGCTT CGAGGTGGAC CTGAAGGATG AGGAGGATGG AGATGGGGAT 
TTAAGTGTAG ATCCAACGGA AGGAACACTA ACACTGGATC TACAAGAAGG TACAAGTGAG 
CCTCAACAGA AAAGTCTCCC GGAGAACGGC GATTTCCCCG CGACCGTGTC CACTTCCTAT 
GTGGATCCTA ACCAACGCGC AAACATCACA AAGGGAAAGG AGAGTCAGGA GCAACCTGTA 
AGTGACTCAC ACCAGCAACC GAATGAAAGC AGCAAGCAAA CCCAAGACTT AAAGGCTGAA 
GAAAGCCAGA CACAAGATCC AGACATTCCC AATGAAGAAG AGGAAGAAGA AGAGGACGAA 
GAAGAGGAAG AAGAGGAAGA GCCGGAAGAC ATTGGTGCCC CCAGTGATAA CCAAGAGGAG 
GGAAAAGAAC CTCTGGAGGA GCAGCCTACC AGCAAGTGGG AAGGAAACAG AGAGCAATCT 
GATGACACCT TAGAAGAGTC CAGTCAGCCC ACTCAGATAA GCAAGACAGA GAAGCATCAA 
TCTGAGCAAG GAAACCAAGG GCAGGAGAGT GACTCTGAGG CAGAAGGAGA GGACAAGGCT 
GCAGGCAGCA AGGAACACAT TCCACATACA GAGCAGCAGG ACCAAGAAGG GAAAGCTGGC 
CTTGAAGCTA TTGGCAACCA GAAGGACACT GATGAGAAGG CCGTTTCCAC AGAACCTACC 
GATGCTGCCG TGGTGCCTAG GAGTCACGGA GGAGCTGGTG ATAACGGGGG CGGGGATGAC 
TCTAAGCATG GTGCAGGCGA TGACTACTTC ATCCCCAGCC AGGAATTCCT AGAGGCCGAA 
AGGATGCATT CCCTCTCCTA TTACCTCAAA TATGGCGGCG GCGAGGAGAC AACGACTGGC 
GAGAGTGAGA ACCGGAGGGA GGCTGCAGAC AACCAAGAGG CCAAGAAAGC TGAGAGCTCA 
CCAAATGCTG AACCTTCAGA TGAGGGCAAC TCAAGGGAGC ACAGTGCTGG TTCTTGCACG 
AACTTCCAAT GTAAAAGGGG ACACATTTGC AAAACCGATC CACAAGGGAA ACCTCACTGT 
GTTTGCCAAG ATCCAGAGAC TTGTCCCCCT GCAAAAATCC TAGATCAGGC TTGTGGCACT 
GACAACCAAA CCTACGCCAG CTCCTGTCAC CTGTTTGCTA CCAAGTGCAG GCTGGAGGGG 
ACCAAAAAGG GACACCAACT GCAGCTGGAT TACTTCGGAG CTTGCAAATC TATTCCTGCT 
TGTACGGACT TTGAAGTGGC TCAGTTTCCC CTGCGGATGA GAGACTGGCT CAAAAACATC 
CTCATGCAGC TTTATGAACC AAATCCCAAA CATGGCGGCT ATCTCAATGA AAAGCAAAGA 
AGCAAAGTCA AAAAAATTTA CCTGGATGAG AAGAGACTCT TGGCTGGAGA CCATCCCATT 
GAACTTCTCT TGAGGGACTT TAAGAAAAAC TACCACATGT ATGTGTATCC TGTGCACTGG 
CAGTTTAATG AACTGGACCA GCATCCTGCA GACAGGATCT TGACACACTC TGAACTTGCT 
CCTCTGCGAG CTTCCCTGGT GCCCATGGAA CACTGCATAA CTCGCTTCTT TGAGGAGTGT 
GACCCCAACA AGGATAAGCA CATCACCTTG AAGGAATGGG GCCACTGCTT TGGAATTAAA 
GAGGAGGATA TAGATGAAAA CCTCCTCTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176145.1
CDS	132..2084
Translation	MKAVLLLLCALGTAVAIPTSTRFLSDHSNPTTATLVTPEDATVPIAGVEATADIENHPSDKAEKPSALNSEEETHEQSTEQDKTYSFEVDLKDEEDGDGDLSVDPTEGTLTLDLQEGTSEPQQKSLPENGDFPATVSTSYVDPNQRANITKGKESQEQPVSDSHQQPNESSKQTQDLKAEESQTQDPDIPNEEEEEEEDEEEEEEEEPEDIGAPSDNQEEGKEPLEEQPTSKWEGNREQSDDTLEESSQPTQISKTEKHQSEQGNQGQESDSEAEGEDKAAGSKEHIPHTEQQDQEGKAGLEAIGNQKDTDEKAVSTEPTDAAVVPRSHGGAGDNGGGDDSKHGAGDDYFIPSQEFLEAERMHSLSYYLKYGGGEETTTGESENRREAADNQEAKKAESSPNAEPSDEGNSREHSAGSCTNFQCKRGHICKTDPQGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPACTDFEVAQFPLRMRDWLKNILMQLYEPNPKHGGYLNEKQRSKVKKIYLDEKRLLAGDHPIELLLRDFKKNYHMYVYPVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

Target ORF information:

RefSeq Version	XM_017320656.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus SPARC-like 1 (Sparcl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320656.1

ORF Insert Sequence:

ATGAAGGCTG TGCTTCTCCT CCTGTGCGCC TTGGGAACCG CTGTGGCAAT CCCGACAAGT 
ACAAGGTTTC TCTCTGACCA CTCCAACCCA ACTACTGCAA CACTGGTGAC ACCGGAAGAC 
GCTACAGTCC CCATTGCCGG GGTTGAAGCT ACAGCAGACA TAGAAAACCA TCCCAGTGAC 
AAGGCTGAAA AACCTTCAGC ACTTAATTCA GAAGAGGAAA CTCATGAACA GTCAACAGAG 
CAGGACAAAA CCTACAGCTT CGAGGTGGAC CTGAAGGATG AGGAGGATGG AGATGGGGAT 
TTAAGTGTAG ATCCAACGGA AGGAACACTA ACACTGGATC TACAAGAAGG TACAAGTGAG 
CCTCAACAGA AAAGTCTCCC GGAGAACGGC GATTTCCCCG CGACCGTGTC CACTTCCTAT 
GTGGATCCTA ACCAACGCGC AAACATCACA AAGGGAAAGG AGAGTCAGGA GCAACCTGTA 
AGTGACTCAC ACCAGCAACC GAATGAAAGC AGCAAGCAAA CCCAAGACTT AAAGGCTGAA 
GAAAGCCAGA CACAAGATCC AGACATTCCC AATGAAGAAG AGGAAGAAGA AGAGGACGAA 
GAAGAGGAAG AAGAGGAAGA GCCGGAAGAC ATTGGTGCCC CCAGTGATAA CCAAGAGGAG 
GGAAAAGAAC CTCTGGAGGA GCAGCCTACC AGCAAGTGGG AAGGAAACAG AGAGCAATCT 
GATGACACCT TAGAAGAGTC CAGTCAGCCC ACTCAGATAA GCAAGACAGA GAAGCATCAA 
TCTGAGCAAG GAAACCAAGG GCAGGAGAGT GACTCTGAGG CAGAAGGAGA GGACAAGGCT 
GCAGGCAGCA AGGAACACAT TCCACATACA GAGCAGCAGG ACCAAGAAGG GAAAGCTGGC 
CTTGAAGCTA TTGGCAACCA GAAGGACACT GATGAGAAGG CCGTTTCCAC AGAACCTACC 
GATGCTGCCG TGGTGCCTAG GAGTCACGGA GGAGCTGGTG ATAACGGGGG CGGGGATGAC 
TCTAAGCATG GTGCAGGCGA TGACTACTTC ATCCCCAGCC AGGAATTCCT AGAGGCCGAA 
AGGATGCATT CCCTCTCCTA TTACCTCAAA TATGGCGGCG GCGAGGAGAC AACGACTGGC 
GAGAGTGAGA ACCGGAGGGA GGCTGCAGAC AACCAAGAGG CCAAGAAAGC TGAGAGCTCA 
CCAAATGCTG AACCTTCAGA TGAGGGCAAC TCAAGGGAGC ACAGTGCTGG TTCTTGCACG 
AACTTCCAAT GTAAAAGGGG ACACATTTGC AAAACCGATC CACAAGGGAA ACCTCACTGT 
GTTTGCCAAG ATCCAGAGAC TTGTCCCCCT GCAAAAATCC TAGATCAGGC TTGTGGCACT 
GACAACCAAA CCTACGCCAG CTCCTGTCAC CTGTTTGCTA CCAAGTGCAG GCTGGAGGGG 
ACCAAAAAGG GACACCAACT GCAGCTGGAT TACTTCGGAG CTTGCAAATC TATTCCTGCT 
TGTACGGACT TTGAAGTGGC TCAGTTTCCC CTGCGGATGA GAGACTGGCT CAAAAACATC 
CTCATGCAGC TTTATGAACC AAATCCCAAA CATGGCGGCT ATCTCAATGA AAAGCAAAGA 
AGCAAAGTCA AAAAAATTTA CCTGGATGAG AAGAGACTCT TGGCTGGAGA CCATCCCATT 
GAACTTCTCT TGAGGGACTT TAAGAAAAAC TACCACATGT ATGTGTATCC TGTGCACTGG 
CAGTTTAATG AACTGGACCA GCATCCTGCA GACAGGATCT TGACACACTC TGAACTTGCT 
CCTCTGCGAG CTTCCCTGGT GCCCATGGAA CACTGCATAA CTCGCTTCTT TGAGGAGTGT 
GACCCCAACA AGGATAAGCA CATCACCTTG AAGGAATGGG GCCACTGCTT TGGAATTAAA 
GAGGAGGATA TAGATGAAAA CCTCCTCTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sparcl1 ( XM_006534768.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534831.1 Mus musculus SPARC-like 1 (Sparcl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu05524
Clone ID Related Accession (Same CDS sequence)	XM_017320656.1 , XM_006534768.1 , NM_010097.4 , NM_001359014.1 , NM_001359016.1
Accession Version	XM_006534768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(House mouse)
Product	SPARC-like protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTG TGCTTCTCCT CCTGTGCGCC TTGGGAACCG CTGTGGCAAT CCCGACAAGT 
ACAAGGTTTC TCTCTGACCA CTCCAACCCA ACTACTGCAA CACTGGTGAC ACCGGAAGAC 
GCTACAGTCC CCATTGCCGG GGTTGAAGCT ACAGCAGACA TAGAAAACCA TCCCAGTGAC 
AAGGCTGAAA AACCTTCAGC ACTTAATTCA GAAGAGGAAA CTCATGAACA GTCAACAGAG 
CAGGACAAAA CCTACAGCTT CGAGGTGGAC CTGAAGGATG AGGAGGATGG AGATGGGGAT 
TTAAGTGTAG ATCCAACGGA AGGAACACTA ACACTGGATC TACAAGAAGG TACAAGTGAG 
CCTCAACAGA AAAGTCTCCC GGAGAACGGC GATTTCCCCG CGACCGTGTC CACTTCCTAT 
GTGGATCCTA ACCAACGCGC AAACATCACA AAGGGAAAGG AGAGTCAGGA GCAACCTGTA 
AGTGACTCAC ACCAGCAACC GAATGAAAGC AGCAAGCAAA CCCAAGACTT AAAGGCTGAA 
GAAAGCCAGA CACAAGATCC AGACATTCCC AATGAAGAAG AGGAAGAAGA AGAGGACGAA 
GAAGAGGAAG AAGAGGAAGA GCCGGAAGAC ATTGGTGCCC CCAGTGATAA CCAAGAGGAG 
GGAAAAGAAC CTCTGGAGGA GCAGCCTACC AGCAAGTGGG AAGGAAACAG AGAGCAATCT 
GATGACACCT TAGAAGAGTC CAGTCAGCCC ACTCAGATAA GCAAGACAGA GAAGCATCAA 
TCTGAGCAAG GAAACCAAGG GCAGGAGAGT GACTCTGAGG CAGAAGGAGA GGACAAGGCT 
GCAGGCAGCA AGGAACACAT TCCACATACA GAGCAGCAGG ACCAAGAAGG GAAAGCTGGC 
CTTGAAGCTA TTGGCAACCA GAAGGACACT GATGAGAAGG CCGTTTCCAC AGAACCTACC 
GATGCTGCCG TGGTGCCTAG GAGTCACGGA GGAGCTGGTG ATAACGGGGG CGGGGATGAC 
TCTAAGCATG GTGCAGGCGA TGACTACTTC ATCCCCAGCC AGGAATTCCT AGAGGCCGAA 
AGGATGCATT CCCTCTCCTA TTACCTCAAA TATGGCGGCG GCGAGGAGAC AACGACTGGC 
GAGAGTGAGA ACCGGAGGGA GGCTGCAGAC AACCAAGAGG CCAAGAAAGC TGAGAGCTCA 
CCAAATGCTG AACCTTCAGA TGAGGGCAAC TCAAGGGAGC ACAGTGCTGG TTCTTGCACG 
AACTTCCAAT GTAAAAGGGG ACACATTTGC AAAACCGATC CACAAGGGAA ACCTCACTGT 
GTTTGCCAAG ATCCAGAGAC TTGTCCCCCT GCAAAAATCC TAGATCAGGC TTGTGGCACT 
GACAACCAAA CCTACGCCAG CTCCTGTCAC CTGTTTGCTA CCAAGTGCAG GCTGGAGGGG 
ACCAAAAAGG GACACCAACT GCAGCTGGAT TACTTCGGAG CTTGCAAATC TATTCCTGCT 
TGTACGGACT TTGAAGTGGC TCAGTTTCCC CTGCGGATGA GAGACTGGCT CAAAAACATC 
CTCATGCAGC TTTATGAACC AAATCCCAAA CATGGCGGCT ATCTCAATGA AAAGCAAAGA 
AGCAAAGTCA AAAAAATTTA CCTGGATGAG AAGAGACTCT TGGCTGGAGA CCATCCCATT 
GAACTTCTCT TGAGGGACTT TAAGAAAAAC TACCACATGT ATGTGTATCC TGTGCACTGG 
CAGTTTAATG AACTGGACCA GCATCCTGCA GACAGGATCT TGACACACTC TGAACTTGCT 
CCTCTGCGAG CTTCCCTGGT GCCCATGGAA CACTGCATAA CTCGCTTCTT TGAGGAGTGT 
GACCCCAACA AGGATAAGCA CATCACCTTG AAGGAATGGG GCCACTGCTT TGGAATTAAA 
GAGGAGGATA TAGATGAAAA CCTCCTCTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534831.1
CDS	897..2849
Translation	MKAVLLLLCALGTAVAIPTSTRFLSDHSNPTTATLVTPEDATVPIAGVEATADIENHPSDKAEKPSALNSEEETHEQSTEQDKTYSFEVDLKDEEDGDGDLSVDPTEGTLTLDLQEGTSEPQQKSLPENGDFPATVSTSYVDPNQRANITKGKESQEQPVSDSHQQPNESSKQTQDLKAEESQTQDPDIPNEEEEEEEDEEEEEEEEPEDIGAPSDNQEEGKEPLEEQPTSKWEGNREQSDDTLEESSQPTQISKTEKHQSEQGNQGQESDSEAEGEDKAAGSKEHIPHTEQQDQEGKAGLEAIGNQKDTDEKAVSTEPTDAAVVPRSHGGAGDNGGGDDSKHGAGDDYFIPSQEFLEAERMHSLSYYLKYGGGEETTTGESENRREAADNQEAKKAESSPNAEPSDEGNSREHSAGSCTNFQCKRGHICKTDPQGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPACTDFEVAQFPLRMRDWLKNILMQLYEPNPKHGGYLNEKQRSKVKKIYLDEKRLLAGDHPIELLLRDFKKNYHMYVYPVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

Target ORF information:

RefSeq Version	XM_006534768.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus SPARC-like 1 (Sparcl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534768.1

ORF Insert Sequence:

ATGAAGGCTG TGCTTCTCCT CCTGTGCGCC TTGGGAACCG CTGTGGCAAT CCCGACAAGT 
ACAAGGTTTC TCTCTGACCA CTCCAACCCA ACTACTGCAA CACTGGTGAC ACCGGAAGAC 
GCTACAGTCC CCATTGCCGG GGTTGAAGCT ACAGCAGACA TAGAAAACCA TCCCAGTGAC 
AAGGCTGAAA AACCTTCAGC ACTTAATTCA GAAGAGGAAA CTCATGAACA GTCAACAGAG 
CAGGACAAAA CCTACAGCTT CGAGGTGGAC CTGAAGGATG AGGAGGATGG AGATGGGGAT 
TTAAGTGTAG ATCCAACGGA AGGAACACTA ACACTGGATC TACAAGAAGG TACAAGTGAG 
CCTCAACAGA AAAGTCTCCC GGAGAACGGC GATTTCCCCG CGACCGTGTC CACTTCCTAT 
GTGGATCCTA ACCAACGCGC AAACATCACA AAGGGAAAGG AGAGTCAGGA GCAACCTGTA 
AGTGACTCAC ACCAGCAACC GAATGAAAGC AGCAAGCAAA CCCAAGACTT AAAGGCTGAA 
GAAAGCCAGA CACAAGATCC AGACATTCCC AATGAAGAAG AGGAAGAAGA AGAGGACGAA 
GAAGAGGAAG AAGAGGAAGA GCCGGAAGAC ATTGGTGCCC CCAGTGATAA CCAAGAGGAG 
GGAAAAGAAC CTCTGGAGGA GCAGCCTACC AGCAAGTGGG AAGGAAACAG AGAGCAATCT 
GATGACACCT TAGAAGAGTC CAGTCAGCCC ACTCAGATAA GCAAGACAGA GAAGCATCAA 
TCTGAGCAAG GAAACCAAGG GCAGGAGAGT GACTCTGAGG CAGAAGGAGA GGACAAGGCT 
GCAGGCAGCA AGGAACACAT TCCACATACA GAGCAGCAGG ACCAAGAAGG GAAAGCTGGC 
CTTGAAGCTA TTGGCAACCA GAAGGACACT GATGAGAAGG CCGTTTCCAC AGAACCTACC 
GATGCTGCCG TGGTGCCTAG GAGTCACGGA GGAGCTGGTG ATAACGGGGG CGGGGATGAC 
TCTAAGCATG GTGCAGGCGA TGACTACTTC ATCCCCAGCC AGGAATTCCT AGAGGCCGAA 
AGGATGCATT CCCTCTCCTA TTACCTCAAA TATGGCGGCG GCGAGGAGAC AACGACTGGC 
GAGAGTGAGA ACCGGAGGGA GGCTGCAGAC AACCAAGAGG CCAAGAAAGC TGAGAGCTCA 
CCAAATGCTG AACCTTCAGA TGAGGGCAAC TCAAGGGAGC ACAGTGCTGG TTCTTGCACG 
AACTTCCAAT GTAAAAGGGG ACACATTTGC AAAACCGATC CACAAGGGAA ACCTCACTGT 
GTTTGCCAAG ATCCAGAGAC TTGTCCCCCT GCAAAAATCC TAGATCAGGC TTGTGGCACT 
GACAACCAAA CCTACGCCAG CTCCTGTCAC CTGTTTGCTA CCAAGTGCAG GCTGGAGGGG 
ACCAAAAAGG GACACCAACT GCAGCTGGAT TACTTCGGAG CTTGCAAATC TATTCCTGCT 
TGTACGGACT TTGAAGTGGC TCAGTTTCCC CTGCGGATGA GAGACTGGCT CAAAAACATC 
CTCATGCAGC TTTATGAACC AAATCCCAAA CATGGCGGCT ATCTCAATGA AAAGCAAAGA 
AGCAAAGTCA AAAAAATTTA CCTGGATGAG AAGAGACTCT TGGCTGGAGA CCATCCCATT 
GAACTTCTCT TGAGGGACTT TAAGAAAAAC TACCACATGT ATGTGTATCC TGTGCACTGG 
CAGTTTAATG AACTGGACCA GCATCCTGCA GACAGGATCT TGACACACTC TGAACTTGCT 
CCTCTGCGAG CTTCCCTGGT GCCCATGGAA CACTGCATAA CTCGCTTCTT TGAGGAGTGT 
GACCCCAACA AGGATAAGCA CATCACCTTG AAGGAATGGG GCCACTGCTT TGGAATTAAA 
GAGGAGGATA TAGATGAAAA CCTCCTCTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SPARCL1 suppresses osteosarcoma metastasis and recruits macrophages by activation of canonical WNT/β-catenin signaling through stabilization of the WNT-receptor complex.
Oncogene37(8)1049-1061(2018 02)
Zhao SJ,Jiang YQ,Xu NW,Li Q,Zhang Q,Wang SY,Li J,Wang YH,Zhang YL,Jiang SH,Wang YJ,Huang YJ,Zhang XX,Tian GA,Zhang CC,Lv YY,Dai M,Liu F,Zhang R,Zhou D,Zhang ZG

Altered Anterior Segment Biometric Parameters in Mice Deficient in SPARC.
Investigative ophthalmology & visual science58(1)386-393(2017 01)
Ho H,Htoon HM,Yam GH,Toh LZ,Lwin NC,Chu S,Lee YS,Wong TT,Seet LF

Spatiotemporal differences in otoconial gene expression.
Genesis (New York, N.Y. : 2000)54(12)613-625(2016 Dec)
Xu Y,Zhang Y,Lundberg YW

Matricellular protein SPARCL1 regulates tumor microenvironment-dependent endothelial cell heterogeneity in colorectal carcinoma.
The Journal of clinical investigation126(11)4187-4204(2016 11)
Naschberger E,Liebl A,Schellerer VS,Schütz M,Britzen-Laurent N,Kölbel P,Schaal U,Haep L,Regensburger D,Wittmann T,Klein-Hitpass L,Rau TT,Dietel B,Méniel VS,Clarke AR,Merkel S,Croner RS,Hohenberger W,Stürzl M

Regulation of adipogenesis by paracrine factors from adipose stromal-vascular fraction - a link to fat depot-specific differences.
Biochimica et biophysica acta1861(9 Pt A)1121-1131(2016 09)
Meissburger B,Perdikari A,Moest H,Müller S,Geiger M,Wolfrum C

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Sparcl1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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