Apc cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Apc
Entrez Gene ID	11789
Full Name	adenomatosis polyposis coli
Synonyms	AI047805,AU020952,AW124434,CC1,Min,mAPC
General protein information	Preferred Names adenomatosis polyposis coli Names adenomatous polyposis coli protein multiple intestinal neoplasia
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 B1\|18 18.53 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006525533.3	XP_006525596.1	adenomatous polyposis coli protein isoform X5
XM_006525539.2	XP_006525602.1	adenomatous polyposis coli protein isoform X9
NM_001360980.1	NP_001347909.1	adenomatous polyposis coli protein isoform 1
NM_007462.3	NP_031488.2	adenomatous polyposis coli protein isoform 3
XM_030250282.1	XP_030106142.1	adenomatous polyposis coli protein isoform X11
XM_030250284.1	XP_030106144.1	adenomatous polyposis coli protein isoform X12
XM_030250287.1	XP_030106147.1	adenomatous polyposis coli protein isoform X14
XM_030250289.1	XP_030106149.1	adenomatous polyposis coli protein isoform X16
XM_030250295.1	XP_030106155.1	adenomatous polyposis coli protein isoform X23
XM_030250296.1	XP_030106156.1	adenomatous polyposis coli protein isoform X23
XM_006525530.1	XP_006525593.1	adenomatous polyposis coli protein isoform X2
XM_006525532.3	XP_006525595.1	adenomatous polyposis coli protein isoform X3
XM_030250291.1	XP_030106151.1	adenomatous polyposis coli protein isoform X18
XM_030250277.1	XP_030106137.1	adenomatous polyposis coli protein isoform X4
XM_030250278.1	XP_030106138.1	adenomatous polyposis coli protein isoform X7
XM_030250281.1	XP_030106141.1	adenomatous polyposis coli protein isoform X10
XM_030250288.1	XP_030106148.1	adenomatous polyposis coli protein isoform X16
XM_006525529.3	XP_006525592.1	adenomatous polyposis coli protein isoform X1
XM_006525533.2	XP_006525596.1	adenomatous polyposis coli protein isoform X4
XM_006525534.4	XP_006525597.1	adenomatous polyposis coli protein isoform X6
XM_006525535.1	XP_006525598.1	adenomatous polyposis coli protein isoform X6
XM_006525538.3	XP_006525601.1	adenomatous polyposis coli protein isoform X8
XM_006525531.1	XP_006525594.1	adenomatous polyposis coli protein isoform X2
XM_006525532.4	XP_006525595.1	adenomatous polyposis coli protein isoform X3
XM_006525539.3	XP_006525602.1	adenomatous polyposis coli protein isoform X20
XM_030250279.1	XP_030106139.1	adenomatous polyposis coli protein isoform X8
XM_030250286.1	XP_030106146.1	adenomatous polyposis coli protein isoform X14
XM_030250290.1	XP_030106150.1	adenomatous polyposis coli protein isoform X17
XM_030250292.1	XP_030106152.1	adenomatous polyposis coli protein isoform X19
XM_006525529.4	XP_006525592.1	adenomatous polyposis coli protein isoform X1
XM_006525538.4	XP_006525601.1	adenomatous polyposis coli protein isoform X15
XM_006525530.2	XP_006525593.1	adenomatous polyposis coli protein isoform X2
XM_006525536.3	XP_006525599.1	adenomatous polyposis coli protein isoform X6
XM_030250280.1	XP_030106140.1	adenomatous polyposis coli protein isoform X9
XM_030250294.1	XP_030106154.1	adenomatous polyposis coli protein isoform X22
NM_001360979.1	NP_001347908.1	adenomatous polyposis coli protein isoform 2
XM_006525534.3	XP_006525597.1	adenomatous polyposis coli protein isoform X5
XM_006525536.4	XP_006525599.1	adenomatous polyposis coli protein isoform X12
XM_017317804.1	XP_017173293.1	adenomatous polyposis coli protein isoform X7
XM_030250283.1	XP_030106143.1	adenomatous polyposis coli protein isoform X12
XM_030250285.1	XP_030106145.1	adenomatous polyposis coli protein isoform X13
XM_030250293.1	XP_030106153.1	adenomatous polyposis coli protein isoform X21

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	APC	XP_005618091.1
Gallus gallus (chicken)	APC	XP_004949340.1
Danio rerio (zebrafish)	apc	NP_001137312.1
Homo sapiens (human)	APC	NP_001120982.1
Bos taurus (cattle)	APC	NP_001069454.2
Mus musculus (house mouse)	Apc	NP_031488.2
Rattus norvegicus (Norway rat)	Apc	NP_036631.1
Xenopus tropicalis (tropical clawed frog)	apc	XP_004910521.1
Pan troglodytes (chimpanzee)	APC	XP_001143965.1

Gene Ontology
Bibliography

Related articles in PubMed

Editing of the gut microbiota reduces carcinogenesis in mouse models of colitis-associated colorectal cancer.
Zhu W, Miyata N, Winter MG, Arenales A, Hughes ER, Spiga L, Kim J, Sifuentes-Dominguez L, Starokadomskyy P, Gopal P, Byndloss MX, Santos RL, Burstein E, Winter SE
The Journal of experimental medicine216(10)2378-2393(2019 Oct)

Epithelial NOTCH Signaling Rewires the Tumor Microenvironment of Colorectal Cancer to Drive Poor-Prognosis Subtypes and Metastasis.
Jackstadt R, van Hooff SR, Leach JD, Cortes-Lavaud X, Lohuis JO, Ridgway RA, Wouters VM, Roper J, Kendall TJ, Roxburgh CS, Horgan PG, Nixon C, Nourse C, Gunzer M, Clark W, Hedley A, Yilmaz OH, Rashid M, Bailey P, Biankin AV, Campbell AD, Adams DJ, Barry ST, Steele CW, Medema JP, Sansom OJ
Cancer cell36(3)319-336.e7(2019 Sep)

TRIM67 Activates p53 to Suppress Colorectal Cancer Initiation and Progression.
Wang S, Zhang Y, Huang J, Wong CC, Zhai J, Li C, Wei G, Zhao L, Wang G, Wei H, Zhao Z, Yu J
Cancer research79(16)4086-4098(2019 Aug)

Determination of the Potential Tumor-Suppressive Effects of
Croes L, Fransen E, Hylebos M, Buys K, Hermans C, Broeckx G, Peeters M, Pauwels P, Op de Beeck K, Van Camp G
Cancers11(8)(2019 Aug)

Lineage tracing and targeting of IL17RB
Goto N, Fukuda A, Yamaga Y, Yoshikawa T, Maruno T, Maekawa H, Inamoto S, Kawada K, Sakai Y, Miyoshi H, Taketo MM, Chiba T, Seno H
Proceedings of the National Academy of Sciences of the United States of America116(26)12996-13005(2019 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Apc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Apc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22391	NM_007462.3 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54318	NM_001360980.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu79754	NM_001360979.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu22391	XM_030250277.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54317	XM_006525529.4 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54318	XM_006525530.2 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54318	XM_006525531.1 Latest version!	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54319	XM_006525532.4 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54320	XM_006525533.3 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54321	XM_006525534.4 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54322	XM_006525536.4 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54322	XM_030250283.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54322	XM_030250284.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54322	XM_006525535.1 Latest version!	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54323	XM_006525538.4 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54324	XM_006525539.3 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu79754	XM_030250286.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu79754	XM_030250287.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu79754	XM_017317804.1 Latest version!	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87321	XM_030250278.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87322	XM_030250279.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87323	XM_030250280.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87324	XM_030250281.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87325	XM_030250282.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87326	XM_030250285.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87327	XM_030250288.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87327	XM_030250289.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87328	XM_030250290.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87329	XM_030250291.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87330	XM_030250292.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87331	XM_030250293.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87332	XM_030250294.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87333	XM_030250295.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu87333	XM_030250296.1 Latest version!	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OMu54320	XM_006525533.2	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54319	XM_006525532.3	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54324	XM_006525539.2	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54317	XM_006525529.3	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54323	XM_006525538.3	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54318	XM_006525530.1	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54321	XM_006525534.3	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu54322	XM_006525536.3	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Apc ( NM_007462.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031488.2 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu22391
Clone ID Related Accession (Same CDS sequence)	NM_007462.3 , XM_030250277.1
Accession Version	NM_007462.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576425600000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK080907.1, AL591373.8, AC090534.10 and BE623286.1. On Nov 1, 2007 this sequence version replaced NM_007462.2. Transcript Variant: This variant (3) differs in the 5' UTR and lacks an exon in the coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK080907.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGGGGACTG CAGGCCATTG CAGAGTTATT GCAGGTGGAC 
TGTGAGATGT ATGGGCTTAC TAATGACCAC TACAGTGTTA CTTTAAGACG GTATGCTGGA 
ATGGCTTTGA CAAACTTGAC CTTTGGAGAT GTTGCCAACA AGGCTACGCT GTGTTCTATG 
AAAGGCTGCA TGAGAGCACT TGTGGCCCAG TTAAAATCTG AGAGTGAAGA CTTACAGCAG 
GTTATTGCAA GTGTTTTGAG GAATTTGTCT TGGCGAGCAG ATGTAAATAG CAAAAAGACG 
TTGAGAGAAG TTGGAAGTGT GAAAGCATTG ATGGAATGTG CTTTGGAAGT TAAAAAGGAA 
TCAACCCTCA AAAGCGTTTT GAGTGCCTTA TGGAACCTGT CTGCACACTG CACTGAGAAT 
AAGGCTGACA TCTGTGCTGT GGATGGAGCA CTGGCATTTC TGGTTGGCAC CCTCACTTAC 
CGGAGCCAGA CAAATACTTT AGCCATTATT GAAAGTGGAG GTGGGATATT ACGGAATGTG 
TCCAGCTTGA TAGCTACAAA CGAAGACCAC AGGCAAATCC TAAGAGAGAA CAATTGCCTA 
CAAACTTTAT TACAGCACTT GAAATCTCAC AGCTTGACAA TAGTCAGTAA TGCATGTGGA 
ACTTTGTGGA ATCTCTCAGC AAGAAATCCT AAAGACCAGG AAGCCTTGTG GGACATGGGG 
GCAGTGAGCA TGCTCAAGAA CCTCATTCAT TCCAAGCACA AAATGATTGC CATGGGAAGT 
GCAGCAGCTT TAAGGAATCT CATGGCAAAC AGACCTGCAA AGTATAAGGA TGCCAATATC 
ATGTCTCCCG GCTCAAGTCT GCCATCCCTT CACGTTAGGA AACAGAAAGC TCTAGAAGCT 
GAGCTAGATG CTCAGCATTT ATCAGAAACC TTCGACAACA TTGACAACCT AAGTCCCAAG 
GCCTCTCACC GGAGTAAGCA GAGACACAAG CAGAATCTTT ATGGTGACTA TGCTTTTGAC 
GCCAATCGAC ATGATGATAG TAGGTCAGAC AATTTCAATA CTGGAAACAT GACTGTTCTT 
TCACCATATT TAAATACTAC GGTATTGCCC AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC 
AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG GAGAGAGAGC GAGGTATTGG CCTCAGTGCT 
TACCATCCAA CAACAGAAAA TGCAGGAACC TCATCAAAAC GAGGTCTGCA GATCACTACC 
ACTGCAGCCC AGATAGCCAA AGTTATGGAA GAAGTATCAG CCATTCATAC CTCCCAGGAC 
GACAGAAGTT CTGCTTCTAC CACCGAGTTC CATTGTGTGG CAGACGACAG GAGTGCGGCA 
CGAAGAAGCT CTGCCTCCCA CACACACTCA AACACATACA ACTTCACTAA GTCGGAAAAT 
TCAAATAGGA CATGCTCTAT GCCTTATGCC AAAGTGGAAT ATAAACGATC TTCAAATGAC 
AGTTTAAATA GTGTCACTAG TAGTGATGGA TATGGTAAAA GAGGCCAAAT GAAACCCTCA 
GTTGAATCCT ATTCTGAAGA TGATGAAAGT AAATTTTGCA GTTATGGTCA GTATCCAGCT 
GACCTAGCCC ATAAGATACA CAGTGCAAAT CATATGGATG ATAATGATGG AGAACTGGAT 
ACACCAATAA ATTACAGTCT TAAATATTCA GATGAGCAGT TGAACTCAGG AAGGCAGAGT 
CCCTCACAGA ATGAAAGGTG GGCAAGACCA AAGCATGTGA TAGAAGATGA AATAAAGCAA 
AACGAGCAAA GACAAGCAAG AAGCCAGAAC ACCAGTTATC CTGTCTATTC TGAGAATACC 
GATGACAAAC ACCTCAAATT CCAACCACAT TTTGGACAAC AAGAATGTGT TTCCCCATAT 
AGGTCAAGGG GAACCAGTGG TTCAGAAACA AATCGAATGG GTTCTAGTCA TGCAATTAAT 
CAAAATGTAA ACCAGTCTCT GTGTCAGGAA GATGATTATG AAGATGATAA ACCTACCAAC 
TACAGTGAAC GTTATTCTGA GGAAGAACAA CATGAAGAAG AAGAAGAGAG ACCGACAAAT 
TATAGCATAA AATATAATGA AGAGAAACAT CATGTGGATC AGCCTATTGA TTATAGTTTA 
AAATATGCCA CTGACATTTC TTCCTCACAA AAACCATCAT TTTCATTCTC AAAGAATTCA 
TCAGCACAAA GCACTAAACC TGAACATCTC TCTCCAAGCA GCGAGAATAC AGCTGTACCT 
CCATCTAATG CCAAAAGGCA GAATCAGCTG CGTCCAAGTT CAGCACAAAG AAATGGCCAG 
ACTCAAAAAG GCACTACTTG CAAAGTCCCC TCCATCAACC AAGAAACAAT ACAGACTTAC 
TGCGTAGAAG ACACCCCAAT ATGTTTTTCA AGGTGCAGTT CATTATCATC ACTGTCATCA 
GCTGACGATG AAATAGGATG TGATCAGACA ACACAGGAAG CAGATTCTGC TAATACTCTG 
CAGACAGCAG AAGTAAAAGA GAATGATGTA ACTCGGTCAG CTGAAGATCC TGCAACTGAA 
GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC AAACCCAGCC GACTCCAGGC TTCTGGCTTA 
TCTTCAGAAT CAACCAGGCA TAATAAAGCT GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC 
TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT CCCCCAGAAC ACTATGTCCA GGAGACTCCG 
CTCGTATTCA GCAGGTGTAC TTCTGTCAGC TCCCTTGACA GTTTTGAGAG TCGCTCCATT 
GCCAGCTCTG TTCAGAGTGA GCCATGTAGT GGAATGGTGA GTGGCATCAT AAGCCCCAGT 
GACCTTCCAG ATAGTCCTGG GCAGACCATG CCACCAAGCA GAAGCAAAAC CCCTCCACCT 
CCTCCACAGA CAGTGCAGGC CAAGAGAGAG GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG 
AAGAGAGAGA GTGGGCCTAA GCAGACTGCT GTAAATGCTG CCGTGCAGAG GGTGCAGGTC 
CTTCCAGACG TGGATACTTT GTTACACTTC GCCACAGAAA GTACTCCAGA CGGGTTTTCT 
TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG GATGAGCCAT TTATACAGAA AGATGTAGAA 
TTAAGAATCA TGCCTCCAGT TCAGGAAAAC GACAATGGGA ATGAAACTGA ATCAGAACAG 
CCTGAGGAAT CAAATGAAAA CCAGGATAAA GAGGTAGAAA AGCCTGACTC TGAAAAAGAC 
TTATTAGATG ATTCTGATGA CGATGATATT GAAATATTAG AAGAATGTAT TATTTCAGCC 
ATGCCAACAA AGTCATCACG CAAAGCCAAA AAACTAGCCC AGACTGCTTC AAAATTACCT 
CCACCTGTGG CAAGGAAACC AAGTCAGCTA CCTGTGTATA AACTTCTGCC AGCACAGAAT 
AGGCTGCAGG CACAAAAACA TGTTAGCTTT ACACCAGGGG ATGATGTGCC CCGGGTGTAC 
TGTGTAGAAG GGACACCTAT AAACTTTTCC ACAGCAACGT CTCTAAGTGA TCTGACAATA 
GAGTCCCCTC CAAATGAATT GGCTACTGGA GATGGGGTCA GAGCGGGTAT ACAGTCAGGT 
GAATTTGAAA AACGAGATAC CATTCCTACA GAAGGCAGAA GTACAGATGA TGCTCAGCGA 
GGAAAAATCT CATCTATAGT TACACCAGAC CTGGATGACA ACAAAGCAGA GGAAGGAGAT 
ATTCTTGCAG AATGTATCAA TTCTGCTATG CCCAAAGGAA AAAGCCACAA GCCTTTCCGA 
GTGAAAAAGA TAATGGACCA AGTCCAACAA GCATCCTCGA CTTCATCTGG AGCTAACAAA 
AATCAAGTAG ACACTAAGAA AAAGAAGCCT ACTTCACCAG TAAAGCCCAT GCCACAAAAT 
ACTGAATATA GAACGCGTGT GAGAAAGAAT ACAGACTCAA AAGTTAATGT AAATACTGAA 
GAAACTTTCT CAGACAACAA AGACTCAAAG AAACCAAGCT TACAAACCAA TGCCAAGGCC 
TTCAATGAAA AGCTACCTAA CAATGAAGAC AGAGTGCGGG GGAGCTTCGC CTTGGACTCA 
CCGCATCACT ACACCCCTAT TGAGGGGACG CCGTACTGCT TTTCCCGAAA TGACTCCTTG 
AGTTCTCTGG ATTTTGATGA TGACGATGTT GACCTTTCCA GGGAAAAGGC CGAGTTAAGA 
AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC AAAGTTACCT GCCGCCCAGA ACCAAACTCA 
AGCCAGCAGG CAGCTAGTAA GTCACAAGCC AGTATAAAAC ATCCAGCAAA CAGAGCACAG 
TCCAAACCAG TGCTGCAGAA ACAGCCCACT TTCCCCCAGT CCTCCAAAGA CGGACCAGAT 
AGAGGGGCAG CAACTGACGA AAAACTGCAG AATTTTGCTA TTGAAAATAC TCCAGTTTGC 
TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT AGTGACATTG ACCAGGAAAA CAACAATAAC 
AAAGAAAGTG AACCAATCAA AGAAGCTGAA CCTGCCAACT CACAAGGAGA GCCCAGTAAG 
CCTCAGGCAT CCGGGTATGC TCCCAAGTCC TTCCACGTCG AAGACACCCC TGTCTGTTTC 
TCAAGAAACA GCTCTCTCAG TTCTCTTAGC ATTGACTCTG AGGACGACCT GTTACAGGAG 
TGTATAAGTT CTGCCATGCC AAAAAAGAAA AGGCCTTCAA GACTCAAGAG TGAGAGCGAA 
AAGCAGAGCC CTAGAAAAGT GGGTGGCATA TTAGCTGAAG ACCTGACGCT TGATTTGAAA 
GATCTACAGA GGCCAGATTC AGAACACGCT TTCTCCCCCG ACTCAGAAAA TTTTGACTGG 
AAAGCTATTC AGGAAGGCGC AAACTCCATA GTAAGTAGTT TGCACCAAGC TGCTGCAGCC 
GCCGCGTGCT TATCTAGACA AGCGTCATCC GACTCAGATT CCATTCTGTC ACTAAAGTCC 
GGCATTTCTC TGGGATCGCC TTTTCATCTT ACACCTGATC AAGAGGAAAA GCCATTCACA 
AGCAATAAAG GCCCAAGAAT TCTCAAACCT GGAGAGAAAA GCACATTAGA AGCAAAAAAA 
ATAGAATCTG AAAACAAAGG AATCAAAGGC GGGAAAAAGG TTTATAAAAG CTTGATTACG 
GGAAAGATTC GCTCCAATTC AGAAATTTCC AGCCAAATGA AACAACCCCT CCCGACAAAC 
ATGCCTTCAA TCTCAAGAGG CAGGACGATG ATTCACATCC CAGGGCTTCG GAATAGCTCC 
TCTAGTACAA GCCCTGTCTC TAAGAAAGGC CCACCCCTCA AGACTCCAGC CTCTAAAAGC 
CCCAGTGAAG GGCCGGGAGC TACCACTTCT CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA 
GAGCTTAGCC CTATCACCAG GCAAACTTCC CAAATCAGTG GGTCAAATAA GGGGTCTTCT 
AGATCAGGAT CTAGAGACTC CACTCCCTCA AGACCTACAC AGCAACCATT AAGTAGGCCA 
ATGCAGTCTC CAGGGCGAAA CTCAATTTCC CCTGGTAGAA ATGGAATAAG CCCTCCTAAC 
AAACTGTCTC AGCTGCCCAG AACATCATCT CCCAGTACTG CTTCAACTAA GTCCTCCGGT 
TCTGGGAAAA TGTCATATAC ATCCCCAGGT AGACAGCTGA GCCAACAAAA TCTTACCAAA 
CAAGCAAGTT TATCCAAGAA TGCCAGCAGT ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA 
CTGAATCAGA TGAGTAACGG CAATGGGTCA AATAAAAAGG TAGAACTTTC TAGAATGTCT 
TCAACTAAAT CAAGTGGAAG TGAATCAGAC AGATCAGAAA GGCCTGCATT AGTACGCCAG 
TCTACTTTCA TCAAAGAAGC CCCAAGCCCA ACCCTGAGGA GGAAACTGGA GGAATCTGCC 
TCATTTGAAT CCCTTTCTCC ATCTTCTAGA CCAGATTCTC CCACCAGGTC GCAGGCACAG 
ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT ATGTCTCTGT CCACACATCC ATCTGTTCAG 
GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT CTCAGCCCCA CTATCGAGTA TAATGACGGA 
AGGCCCACAA AACGGCATGA TATTGCACGC TCCCATTCTG AAAGTCCTTC CAGACTACCA 
ATCAACCGGG CGGGAACCTG GAAGCGTGAA CACAGCAAAC ATTCCTCGTC CCTTCCTCGA 
GTGAGTACTT GGAGAAGAAC TGGAAGCTCA TCTTCTATTC TTTCTGCTTC ATCAGAGTCC 
AGTGAAAAAG CAAAAAGTGA GGATGAAAGG CATGTGAGCT CCATGCCAGC ACCCAGACAG 
ATGAAGGAAA ACCAGGTGCC CACCAAAGGA ACATGGAGGA AAATCAAGGA AAGTGACATT 
TCTCCCACAG GCATGGCTTC TCAGAGCGCT TCCTCAGGTG CTGCCAGTGG TGCTGAATCC 
AAGCCTCTGA TCTATCAGAT GGCACCTCCT GTCTCTAAAA CAGAGGATGT TTGGGTGAGA 
ATTGAGGACT GCCCCATTAA CAACCCTAGA TCTGGACGGT CCCCCACAGG CAACACCCCC 
CCAGTGATTG ACAGTGTTTC AGAGAAGGGA AGTTCAAGCA TTAAAGATTC AAAAGACACC 
CATGGGAAAC AGAGTGTGGG CAGTGGCAGT CCTGTGCAAA CCGTGGGTCT GGAAACCCGC 
CTCAACTCCT TTGTTCAGGT AGAGGCCCCA GAACAGAAAG GAACTGAGGC AAAACCAGGA 
CAGAGTAACC CAGTCTCTAT AGCAGAGACT GCTGAGACGT GTATAGCAGA GCGTACCCCT 
TTCAGTTCCA GTAGCTCCAG CAAGCACAGC TCACCTAGCG GGACTGTTGC TGCCAGAGTG 
ACACCTTTTA ATTACAACCC TAGCCCTAGG AAGAGCAGCG CAGACAGCAC TTCAGCCCGG 
CCGTCTCAGA TCCCTACGCC AGTGAGCACC AACACGAAGA AGAGAGATTC GAAGACTGAC 
AGCACAGAAT CCAGTGGAGC CCAAAGTCCT AAACGCCATT CCGGGTCTTA CCTCGTGACG 
TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031488.2
CDS	312..8840
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	NM_007462.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007462.3

ORF Insert Sequence:

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGGGGACTG CAGGCCATTG CAGAGTTATT GCAGGTGGAC 
TGTGAGATGT ATGGGCTTAC TAATGACCAC TACAGTGTTA CTTTAAGACG GTATGCTGGA 
ATGGCTTTGA CAAACTTGAC CTTTGGAGAT GTTGCCAACA AGGCTACGCT GTGTTCTATG 
AAAGGCTGCA TGAGAGCACT TGTGGCCCAG TTAAAATCTG AGAGTGAAGA CTTACAGCAG 
GTTATTGCAA GTGTTTTGAG GAATTTGTCT TGGCGAGCAG ATGTAAATAG CAAAAAGACG 
TTGAGAGAAG TTGGAAGTGT GAAAGCATTG ATGGAATGTG CTTTGGAAGT TAAAAAGGAA 
TCAACCCTCA AAAGCGTTTT GAGTGCCTTA TGGAACCTGT CTGCACACTG CACTGAGAAT 
AAGGCTGACA TCTGTGCTGT GGATGGAGCA CTGGCATTTC TGGTTGGCAC CCTCACTTAC 
CGGAGCCAGA CAAATACTTT AGCCATTATT GAAAGTGGAG GTGGGATATT ACGGAATGTG 
TCCAGCTTGA TAGCTACAAA CGAAGACCAC AGGCAAATCC TAAGAGAGAA CAATTGCCTA 
CAAACTTTAT TACAGCACTT GAAATCTCAC AGCTTGACAA TAGTCAGTAA TGCATGTGGA 
ACTTTGTGGA ATCTCTCAGC AAGAAATCCT AAAGACCAGG AAGCCTTGTG GGACATGGGG 
GCAGTGAGCA TGCTCAAGAA CCTCATTCAT TCCAAGCACA AAATGATTGC CATGGGAAGT 
GCAGCAGCTT TAAGGAATCT CATGGCAAAC AGACCTGCAA AGTATAAGGA TGCCAATATC 
ATGTCTCCCG GCTCAAGTCT GCCATCCCTT CACGTTAGGA AACAGAAAGC TCTAGAAGCT 
GAGCTAGATG CTCAGCATTT ATCAGAAACC TTCGACAACA TTGACAACCT AAGTCCCAAG 
GCCTCTCACC GGAGTAAGCA GAGACACAAG CAGAATCTTT ATGGTGACTA TGCTTTTGAC 
GCCAATCGAC ATGATGATAG TAGGTCAGAC AATTTCAATA CTGGAAACAT GACTGTTCTT 
TCACCATATT TAAATACTAC GGTATTGCCC AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC 
AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG GAGAGAGAGC GAGGTATTGG CCTCAGTGCT 
TACCATCCAA CAACAGAAAA TGCAGGAACC TCATCAAAAC GAGGTCTGCA GATCACTACC 
ACTGCAGCCC AGATAGCCAA AGTTATGGAA GAAGTATCAG CCATTCATAC CTCCCAGGAC 
GACAGAAGTT CTGCTTCTAC CACCGAGTTC CATTGTGTGG CAGACGACAG GAGTGCGGCA 
CGAAGAAGCT CTGCCTCCCA CACACACTCA AACACATACA ACTTCACTAA GTCGGAAAAT 
TCAAATAGGA CATGCTCTAT GCCTTATGCC AAAGTGGAAT ATAAACGATC TTCAAATGAC 
AGTTTAAATA GTGTCACTAG TAGTGATGGA TATGGTAAAA GAGGCCAAAT GAAACCCTCA 
GTTGAATCCT ATTCTGAAGA TGATGAAAGT AAATTTTGCA GTTATGGTCA GTATCCAGCT 
GACCTAGCCC ATAAGATACA CAGTGCAAAT CATATGGATG ATAATGATGG AGAACTGGAT 
ACACCAATAA ATTACAGTCT TAAATATTCA GATGAGCAGT TGAACTCAGG AAGGCAGAGT 
CCCTCACAGA ATGAAAGGTG GGCAAGACCA AAGCATGTGA TAGAAGATGA AATAAAGCAA 
AACGAGCAAA GACAAGCAAG AAGCCAGAAC ACCAGTTATC CTGTCTATTC TGAGAATACC 
GATGACAAAC ACCTCAAATT CCAACCACAT TTTGGACAAC AAGAATGTGT TTCCCCATAT 
AGGTCAAGGG GAACCAGTGG TTCAGAAACA AATCGAATGG GTTCTAGTCA TGCAATTAAT 
CAAAATGTAA ACCAGTCTCT GTGTCAGGAA GATGATTATG AAGATGATAA ACCTACCAAC 
TACAGTGAAC GTTATTCTGA GGAAGAACAA CATGAAGAAG AAGAAGAGAG ACCGACAAAT 
TATAGCATAA AATATAATGA AGAGAAACAT CATGTGGATC AGCCTATTGA TTATAGTTTA 
AAATATGCCA CTGACATTTC TTCCTCACAA AAACCATCAT TTTCATTCTC AAAGAATTCA 
TCAGCACAAA GCACTAAACC TGAACATCTC TCTCCAAGCA GCGAGAATAC AGCTGTACCT 
CCATCTAATG CCAAAAGGCA GAATCAGCTG CGTCCAAGTT CAGCACAAAG AAATGGCCAG 
ACTCAAAAAG GCACTACTTG CAAAGTCCCC TCCATCAACC AAGAAACAAT ACAGACTTAC 
TGCGTAGAAG ACACCCCAAT ATGTTTTTCA AGGTGCAGTT CATTATCATC ACTGTCATCA 
GCTGACGATG AAATAGGATG TGATCAGACA ACACAGGAAG CAGATTCTGC TAATACTCTG 
CAGACAGCAG AAGTAAAAGA GAATGATGTA ACTCGGTCAG CTGAAGATCC TGCAACTGAA 
GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC AAACCCAGCC GACTCCAGGC TTCTGGCTTA 
TCTTCAGAAT CAACCAGGCA TAATAAAGCT GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC 
TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT CCCCCAGAAC ACTATGTCCA GGAGACTCCG 
CTCGTATTCA GCAGGTGTAC TTCTGTCAGC TCCCTTGACA GTTTTGAGAG TCGCTCCATT 
GCCAGCTCTG TTCAGAGTGA GCCATGTAGT GGAATGGTGA GTGGCATCAT AAGCCCCAGT 
GACCTTCCAG ATAGTCCTGG GCAGACCATG CCACCAAGCA GAAGCAAAAC CCCTCCACCT 
CCTCCACAGA CAGTGCAGGC CAAGAGAGAG GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG 
AAGAGAGAGA GTGGGCCTAA GCAGACTGCT GTAAATGCTG CCGTGCAGAG GGTGCAGGTC 
CTTCCAGACG TGGATACTTT GTTACACTTC GCCACAGAAA GTACTCCAGA CGGGTTTTCT 
TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG GATGAGCCAT TTATACAGAA AGATGTAGAA 
TTAAGAATCA TGCCTCCAGT TCAGGAAAAC GACAATGGGA ATGAAACTGA ATCAGAACAG 
CCTGAGGAAT CAAATGAAAA CCAGGATAAA GAGGTAGAAA AGCCTGACTC TGAAAAAGAC 
TTATTAGATG ATTCTGATGA CGATGATATT GAAATATTAG AAGAATGTAT TATTTCAGCC 
ATGCCAACAA AGTCATCACG CAAAGCCAAA AAACTAGCCC AGACTGCTTC AAAATTACCT 
CCACCTGTGG CAAGGAAACC AAGTCAGCTA CCTGTGTATA AACTTCTGCC AGCACAGAAT 
AGGCTGCAGG CACAAAAACA TGTTAGCTTT ACACCAGGGG ATGATGTGCC CCGGGTGTAC 
TGTGTAGAAG GGACACCTAT AAACTTTTCC ACAGCAACGT CTCTAAGTGA TCTGACAATA 
GAGTCCCCTC CAAATGAATT GGCTACTGGA GATGGGGTCA GAGCGGGTAT ACAGTCAGGT 
GAATTTGAAA AACGAGATAC CATTCCTACA GAAGGCAGAA GTACAGATGA TGCTCAGCGA 
GGAAAAATCT CATCTATAGT TACACCAGAC CTGGATGACA ACAAAGCAGA GGAAGGAGAT 
ATTCTTGCAG AATGTATCAA TTCTGCTATG CCCAAAGGAA AAAGCCACAA GCCTTTCCGA 
GTGAAAAAGA TAATGGACCA AGTCCAACAA GCATCCTCGA CTTCATCTGG AGCTAACAAA 
AATCAAGTAG ACACTAAGAA AAAGAAGCCT ACTTCACCAG TAAAGCCCAT GCCACAAAAT 
ACTGAATATA GAACGCGTGT GAGAAAGAAT ACAGACTCAA AAGTTAATGT AAATACTGAA 
GAAACTTTCT CAGACAACAA AGACTCAAAG AAACCAAGCT TACAAACCAA TGCCAAGGCC 
TTCAATGAAA AGCTACCTAA CAATGAAGAC AGAGTGCGGG GGAGCTTCGC CTTGGACTCA 
CCGCATCACT ACACCCCTAT TGAGGGGACG CCGTACTGCT TTTCCCGAAA TGACTCCTTG 
AGTTCTCTGG ATTTTGATGA TGACGATGTT GACCTTTCCA GGGAAAAGGC CGAGTTAAGA 
AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC AAAGTTACCT GCCGCCCAGA ACCAAACTCA 
AGCCAGCAGG CAGCTAGTAA GTCACAAGCC AGTATAAAAC ATCCAGCAAA CAGAGCACAG 
TCCAAACCAG TGCTGCAGAA ACAGCCCACT TTCCCCCAGT CCTCCAAAGA CGGACCAGAT 
AGAGGGGCAG CAACTGACGA AAAACTGCAG AATTTTGCTA TTGAAAATAC TCCAGTTTGC 
TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT AGTGACATTG ACCAGGAAAA CAACAATAAC 
AAAGAAAGTG AACCAATCAA AGAAGCTGAA CCTGCCAACT CACAAGGAGA GCCCAGTAAG 
CCTCAGGCAT CCGGGTATGC TCCCAAGTCC TTCCACGTCG AAGACACCCC TGTCTGTTTC 
TCAAGAAACA GCTCTCTCAG TTCTCTTAGC ATTGACTCTG AGGACGACCT GTTACAGGAG 
TGTATAAGTT CTGCCATGCC AAAAAAGAAA AGGCCTTCAA GACTCAAGAG TGAGAGCGAA 
AAGCAGAGCC CTAGAAAAGT GGGTGGCATA TTAGCTGAAG ACCTGACGCT TGATTTGAAA 
GATCTACAGA GGCCAGATTC AGAACACGCT TTCTCCCCCG ACTCAGAAAA TTTTGACTGG 
AAAGCTATTC AGGAAGGCGC AAACTCCATA GTAAGTAGTT TGCACCAAGC TGCTGCAGCC 
GCCGCGTGCT TATCTAGACA AGCGTCATCC GACTCAGATT CCATTCTGTC ACTAAAGTCC 
GGCATTTCTC TGGGATCGCC TTTTCATCTT ACACCTGATC AAGAGGAAAA GCCATTCACA 
AGCAATAAAG GCCCAAGAAT TCTCAAACCT GGAGAGAAAA GCACATTAGA AGCAAAAAAA 
ATAGAATCTG AAAACAAAGG AATCAAAGGC GGGAAAAAGG TTTATAAAAG CTTGATTACG 
GGAAAGATTC GCTCCAATTC AGAAATTTCC AGCCAAATGA AACAACCCCT CCCGACAAAC 
ATGCCTTCAA TCTCAAGAGG CAGGACGATG ATTCACATCC CAGGGCTTCG GAATAGCTCC 
TCTAGTACAA GCCCTGTCTC TAAGAAAGGC CCACCCCTCA AGACTCCAGC CTCTAAAAGC 
CCCAGTGAAG GGCCGGGAGC TACCACTTCT CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA 
GAGCTTAGCC CTATCACCAG GCAAACTTCC CAAATCAGTG GGTCAAATAA GGGGTCTTCT 
AGATCAGGAT CTAGAGACTC CACTCCCTCA AGACCTACAC AGCAACCATT AAGTAGGCCA 
ATGCAGTCTC CAGGGCGAAA CTCAATTTCC CCTGGTAGAA ATGGAATAAG CCCTCCTAAC 
AAACTGTCTC AGCTGCCCAG AACATCATCT CCCAGTACTG CTTCAACTAA GTCCTCCGGT 
TCTGGGAAAA TGTCATATAC ATCCCCAGGT AGACAGCTGA GCCAACAAAA TCTTACCAAA 
CAAGCAAGTT TATCCAAGAA TGCCAGCAGT ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA 
CTGAATCAGA TGAGTAACGG CAATGGGTCA AATAAAAAGG TAGAACTTTC TAGAATGTCT 
TCAACTAAAT CAAGTGGAAG TGAATCAGAC AGATCAGAAA GGCCTGCATT AGTACGCCAG 
TCTACTTTCA TCAAAGAAGC CCCAAGCCCA ACCCTGAGGA GGAAACTGGA GGAATCTGCC 
TCATTTGAAT CCCTTTCTCC ATCTTCTAGA CCAGATTCTC CCACCAGGTC GCAGGCACAG 
ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT ATGTCTCTGT CCACACATCC ATCTGTTCAG 
GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT CTCAGCCCCA CTATCGAGTA TAATGACGGA 
AGGCCCACAA AACGGCATGA TATTGCACGC TCCCATTCTG AAAGTCCTTC CAGACTACCA 
ATCAACCGGG CGGGAACCTG GAAGCGTGAA CACAGCAAAC ATTCCTCGTC CCTTCCTCGA 
GTGAGTACTT GGAGAAGAAC TGGAAGCTCA TCTTCTATTC TTTCTGCTTC ATCAGAGTCC 
AGTGAAAAAG CAAAAAGTGA GGATGAAAGG CATGTGAGCT CCATGCCAGC ACCCAGACAG 
ATGAAGGAAA ACCAGGTGCC CACCAAAGGA ACATGGAGGA AAATCAAGGA AAGTGACATT 
TCTCCCACAG GCATGGCTTC TCAGAGCGCT TCCTCAGGTG CTGCCAGTGG TGCTGAATCC 
AAGCCTCTGA TCTATCAGAT GGCACCTCCT GTCTCTAAAA CAGAGGATGT TTGGGTGAGA 
ATTGAGGACT GCCCCATTAA CAACCCTAGA TCTGGACGGT CCCCCACAGG CAACACCCCC 
CCAGTGATTG ACAGTGTTTC AGAGAAGGGA AGTTCAAGCA TTAAAGATTC AAAAGACACC 
CATGGGAAAC AGAGTGTGGG CAGTGGCAGT CCTGTGCAAA CCGTGGGTCT GGAAACCCGC 
CTCAACTCCT TTGTTCAGGT AGAGGCCCCA GAACAGAAAG GAACTGAGGC AAAACCAGGA 
CAGAGTAACC CAGTCTCTAT AGCAGAGACT GCTGAGACGT GTATAGCAGA GCGTACCCCT 
TTCAGTTCCA GTAGCTCCAG CAAGCACAGC TCACCTAGCG GGACTGTTGC TGCCAGAGTG 
ACACCTTTTA ATTACAACCC TAGCCCTAGG AAGAGCAGCG CAGACAGCAC TTCAGCCCGG 
CCGTCTCAGA TCCCTACGCC AGTGAGCACC AACACGAAGA AGAGAGATTC GAAGACTGAC 
AGCACAGAAT CCAGTGGAGC CCAAAGTCCT AAACGCCATT CCGGGTCTTA CCTCGTGACG 
TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( NM_001360980.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347909.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant 1, mRNA.

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CloneID	OMu54318
Clone ID Related Accession (Same CDS sequence)	NM_001360980.1 , XM_006525530.1 , XM_006525531.1 , XM_006525530.2
Accession Version	NM_001360980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576425600000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114003.4, AL591373.8 and AC090534.10. On Feb 23, 2018 this sequence version replaced XM_006525531.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.32158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849387 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGTAGGAAG GCTACCCGGG GCATTTCATC CCAGGAGCTA 
GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC ATTGCAGAGT TATTGCAGGT GGACTGTGAG 
ATGTATGGGC TTACTAATGA CCACTACAGT GTTACTTTAA GACGGTATGC TGGAATGGCT 
TTGACAAACT TGACCTTTGG AGATGTTGCC AACAAGGCTA CGCTGTGTTC TATGAAAGGC 
TGCATGAGAG CACTTGTGGC CCAGTTAAAA TCTGAGAGTG AAGACTTACA GCAGGTTATT 
GCAAGTGTTT TGAGGAATTT GTCTTGGCGA GCAGATGTAA ATAGCAAAAA GACGTTGAGA 
GAAGTTGGAA GTGTGAAAGC ATTGATGGAA TGTGCTTTGG AAGTTAAAAA GGAATCAACC 
CTCAAAAGCG TTTTGAGTGC CTTATGGAAC CTGTCTGCAC ACTGCACTGA GAATAAGGCT 
GACATCTGTG CTGTGGATGG AGCACTGGCA TTTCTGGTTG GCACCCTCAC TTACCGGAGC 
CAGACAAATA CTTTAGCCAT TATTGAAAGT GGAGGTGGGA TATTACGGAA TGTGTCCAGC 
TTGATAGCTA CAAACGAAGA CCACAGGCAA ATCCTAAGAG AGAACAATTG CCTACAAACT 
TTATTACAGC ACTTGAAATC TCACAGCTTG ACAATAGTCA GTAATGCATG TGGAACTTTG 
TGGAATCTCT CAGCAAGAAA TCCTAAAGAC CAGGAAGCCT TGTGGGACAT GGGGGCAGTG 
AGCATGCTCA AGAACCTCAT TCATTCCAAG CACAAAATGA TTGCCATGGG AAGTGCAGCA 
GCTTTAAGGA ATCTCATGGC AAACAGACCT GCAAAGTATA AGGATGCCAA TATCATGTCT 
CCCGGCTCAA GTCTGCCATC CCTTCACGTT AGGAAACAGA AAGCTCTAGA AGCTGAGCTA 
GATGCTCAGC ATTTATCAGA AACCTTCGAC AACATTGACA ACCTAAGTCC CAAGGCCTCT 
CACCGGAGTA AGCAGAGACA CAAGCAGAAT CTTTATGGTG ACTATGCTTT TGACGCCAAT 
CGACATGATG ATAGTAGGTC AGACAATTTC AATACTGGAA ACATGACTGT TCTTTCACCA 
TATTTAAATA CTACGGTATT GCCCAGCTCT TCTTCCTCAA GGGGAAGTTT AGACAGTTCT 
CGTTCTGAGA AAGACAGAAG TTTGGAGAGA GAGCGAGGTA TTGGCCTCAG TGCTTACCAT 
CCAACAACAG AAAATGCAGG AACCTCATCA AAACGAGGTC TGCAGATCAC TACCACTGCA 
GCCCAGATAG CCAAAGTTAT GGAAGAAGTA TCAGCCATTC ATACCTCCCA GGACGACAGA 
AGTTCTGCTT CTACCACCGA GTTCCATTGT GTGGCAGACG ACAGGAGTGC GGCACGAAGA 
AGCTCTGCCT CCCACACACA CTCAAACACA TACAACTTCA CTAAGTCGGA AAATTCAAAT 
AGGACATGCT CTATGCCTTA TGCCAAAGTG GAATATAAAC GATCTTCAAA TGACAGTTTA 
AATAGTGTCA CTAGTAGTGA TGGATATGGT AAAAGAGGCC AAATGAAACC CTCAGTTGAA 
TCCTATTCTG AAGATGATGA AAGTAAATTT TGCAGTTATG GTCAGTATCC AGCTGACCTA 
GCCCATAAGA TACACAGTGC AAATCATATG GATGATAATG ATGGAGAACT GGATACACCA 
ATAAATTACA GTCTTAAATA TTCAGATGAG CAGTTGAACT CAGGAAGGCA GAGTCCCTCA 
CAGAATGAAA GGTGGGCAAG ACCAAAGCAT GTGATAGAAG ATGAAATAAA GCAAAACGAG 
CAAAGACAAG CAAGAAGCCA GAACACCAGT TATCCTGTCT ATTCTGAGAA TACCGATGAC 
AAACACCTCA AATTCCAACC ACATTTTGGA CAACAAGAAT GTGTTTCCCC ATATAGGTCA 
AGGGGAACCA GTGGTTCAGA AACAAATCGA ATGGGTTCTA GTCATGCAAT TAATCAAAAT 
GTAAACCAGT CTCTGTGTCA GGAAGATGAT TATGAAGATG ATAAACCTAC CAACTACAGT 
GAACGTTATT CTGAGGAAGA ACAACATGAA GAAGAAGAAG AGAGACCGAC AAATTATAGC 
ATAAAATATA ATGAAGAGAA ACATCATGTG GATCAGCCTA TTGATTATAG TTTAAAATAT 
GCCACTGACA TTTCTTCCTC ACAAAAACCA TCATTTTCAT TCTCAAAGAA TTCATCAGCA 
CAAAGCACTA AACCTGAACA TCTCTCTCCA AGCAGCGAGA ATACAGCTGT ACCTCCATCT 
AATGCCAAAA GGCAGAATCA GCTGCGTCCA AGTTCAGCAC AAAGAAATGG CCAGACTCAA 
AAAGGCACTA CTTGCAAAGT CCCCTCCATC AACCAAGAAA CAATACAGAC TTACTGCGTA 
GAAGACACCC CAATATGTTT TTCAAGGTGC AGTTCATTAT CATCACTGTC ATCAGCTGAC 
GATGAAATAG GATGTGATCA GACAACACAG GAAGCAGATT CTGCTAATAC TCTGCAGACA 
GCAGAAGTAA AAGAGAATGA TGTAACTCGG TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA 
GCAGTGTCCC AGAATGCTAG AGCCAAACCC AGCCGACTCC AGGCTTCTGG CTTATCTTCA 
GAATCAACCA GGCATAATAA AGCTGTTGAG TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA 
AGTGGTGCTC AGACACCCAA AAGTCCCCCA GAACACTATG TCCAGGAGAC TCCGCTCGTA 
TTCAGCAGGT GTACTTCTGT CAGCTCCCTT GACAGTTTTG AGAGTCGCTC CATTGCCAGC 
TCTGTTCAGA GTGAGCCATG TAGTGGAATG GTGAGTGGCA TCATAAGCCC CAGTGACCTT 
CCAGATAGTC CTGGGCAGAC CATGCCACCA AGCAGAAGCA AAACCCCTCC ACCTCCTCCA 
CAGACAGTGC AGGCCAAGAG AGAGGTGCCA AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA 
GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA 
GACGTGGATA CTTTGTTACA CTTCGCCACA GAAAGTACTC CAGACGGGTT TTCTTGTTCC 
TCCAGCCTAA GTGCTCTGAG CCTGGATGAG CCATTTATAC AGAAAGATGT AGAATTAAGA 
ATCATGCCTC CAGTTCAGGA AAACGACAAT GGGAATGAAA CTGAATCAGA ACAGCCTGAG 
GAATCAAATG AAAACCAGGA TAAAGAGGTA GAAAAGCCTG ACTCTGAAAA AGACTTATTA 
GATGATTCTG ATGACGATGA TATTGAAATA TTAGAAGAAT GTATTATTTC AGCCATGCCA 
ACAAAGTCAT CACGCAAAGC CAAAAAACTA GCCCAGACTG CTTCAAAATT ACCTCCACCT 
GTGGCAAGGA AACCAAGTCA GCTACCTGTG TATAAACTTC TGCCAGCACA GAATAGGCTG 
CAGGCACAAA AACATGTTAG CTTTACACCA GGGGATGATG TGCCCCGGGT GTACTGTGTA 
GAAGGGACAC CTATAAACTT TTCCACAGCA ACGTCTCTAA GTGATCTGAC AATAGAGTCC 
CCTCCAAATG AATTGGCTAC TGGAGATGGG GTCAGAGCGG GTATACAGTC AGGTGAATTT 
GAAAAACGAG ATACCATTCC TACAGAAGGC AGAAGTACAG ATGATGCTCA GCGAGGAAAA 
ATCTCATCTA TAGTTACACC AGACCTGGAT GACAACAAAG CAGAGGAAGG AGATATTCTT 
GCAGAATGTA TCAATTCTGC TATGCCCAAA GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA 
AAGATAATGG ACCAAGTCCA ACAAGCATCC TCGACTTCAT CTGGAGCTAA CAAAAATCAA 
GTAGACACTA AGAAAAAGAA GCCTACTTCA CCAGTAAAGC CCATGCCACA AAATACTGAA 
TATAGAACGC GTGTGAGAAA GAATACAGAC TCAAAAGTTA ATGTAAATAC TGAAGAAACT 
TTCTCAGACA ACAAAGACTC AAAGAAACCA AGCTTACAAA CCAATGCCAA GGCCTTCAAT 
GAAAAGCTAC CTAACAATGA AGACAGAGTG CGGGGGAGCT TCGCCTTGGA CTCACCGCAT 
CACTACACCC CTATTGAGGG GACGCCGTAC TGCTTTTCCC GAAATGACTC CTTGAGTTCT 
CTGGATTTTG ATGATGACGA TGTTGACCTT TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC 
AAAGAAAGCA AGGATTCCGA AGCCAAAGTT ACCTGCCGCC CAGAACCAAA CTCAAGCCAG 
CAGGCAGCTA GTAAGTCACA AGCCAGTATA AAACATCCAG CAAACAGAGC ACAGTCCAAA 
CCAGTGCTGC AGAAACAGCC CACTTTCCCC CAGTCCTCCA AAGACGGACC AGATAGAGGG 
GCAGCAACTG ACGAAAAACT GCAGAATTTT GCTATTGAAA ATACTCCAGT TTGCTTTTCT 
CGAAATTCCT CTCTGAGTTC CCTTAGTGAC ATTGACCAGG AAAACAACAA TAACAAAGAA 
AGTGAACCAA TCAAAGAAGC TGAACCTGCC AACTCACAAG GAGAGCCCAG TAAGCCTCAG 
GCATCCGGGT ATGCTCCCAA GTCCTTCCAC GTCGAAGACA CCCCTGTCTG TTTCTCAAGA 
AACAGCTCTC TCAGTTCTCT TAGCATTGAC TCTGAGGACG ACCTGTTACA GGAGTGTATA 
AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT TCAAGACTCA AGAGTGAGAG CGAAAAGCAG 
AGCCCTAGAA AAGTGGGTGG CATATTAGCT GAAGACCTGA CGCTTGATTT GAAAGATCTA 
CAGAGGCCAG ATTCAGAACA CGCTTTCTCC CCCGACTCAG AAAATTTTGA CTGGAAAGCT 
ATTCAGGAAG GCGCAAACTC CATAGTAAGT AGTTTGCACC AAGCTGCTGC AGCCGCCGCG 
TGCTTATCTA GACAAGCGTC ATCCGACTCA GATTCCATTC TGTCACTAAA GTCCGGCATT 
TCTCTGGGAT CGCCTTTTCA TCTTACACCT GATCAAGAGG AAAAGCCATT CACAAGCAAT 
AAAGGCCCAA GAATTCTCAA ACCTGGAGAG AAAAGCACAT TAGAAGCAAA AAAAATAGAA 
TCTGAAAACA AAGGAATCAA AGGCGGGAAA AAGGTTTATA AAAGCTTGAT TACGGGAAAG 
ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA ATGAAACAAC CCCTCCCGAC AAACATGCCT 
TCAATCTCAA GAGGCAGGAC GATGATTCAC ATCCCAGGGC TTCGGAATAG CTCCTCTAGT 
ACAAGCCCTG TCTCTAAGAA AGGCCCACCC CTCAAGACTC CAGCCTCTAA AAGCCCCAGT 
GAAGGGCCGG GAGCTACCAC TTCTCCTCGA GGAACTAAGC CAGCAGGAAA GTCAGAGCTT 
AGCCCTATCA CCAGGCAAAC TTCCCAAATC AGTGGGTCAA ATAAGGGGTC TTCTAGATCA 
GGATCTAGAG ACTCCACTCC CTCAAGACCT ACACAGCAAC CATTAAGTAG GCCAATGCAG 
TCTCCAGGGC GAAACTCAAT TTCCCCTGGT AGAAATGGAA TAAGCCCTCC TAACAAACTG 
TCTCAGCTGC CCAGAACATC ATCTCCCAGT ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG 
AAAATGTCAT ATACATCCCC AGGTAGACAG CTGAGCCAAC AAAATCTTAC CAAACAAGCA 
AGTTTATCCA AGAATGCCAG CAGTATCCCC AGAAGTGAGT CGGCATCTAA AGGACTGAAT 
CAGATGAGTA ACGGCAATGG GTCAAATAAA AAGGTAGAAC TTTCTAGAAT GTCTTCAACT 
AAATCAAGTG GAAGTGAATC AGACAGATCA GAAAGGCCTG CATTAGTACG CCAGTCTACT 
TTCATCAAAG AAGCCCCAAG CCCAACCCTG AGGAGGAAAC TGGAGGAATC TGCCTCATTT 
GAATCCCTTT CTCCATCTTC TAGACCAGAT TCTCCCACCA GGTCGCAGGC ACAGACCCCA 
GTTTTAAGCC CTTCCCTTCC TGATATGTCT CTGTCCACAC ATCCATCTGT TCAGGCAGGT 
GGGTGGCGAA AGCTCCCGCC TAATCTCAGC CCCACTATCG AGTATAATGA CGGAAGGCCC 
ACAAAACGGC ATGATATTGC ACGCTCCCAT TCTGAAAGTC CTTCCAGACT ACCAATCAAC 
CGGGCGGGAA CCTGGAAGCG TGAACACAGC AAACATTCCT CGTCCCTTCC TCGAGTGAGT 
ACTTGGAGAA GAACTGGAAG CTCATCTTCT ATTCTTTCTG CTTCATCAGA GTCCAGTGAA 
AAAGCAAAAA GTGAGGATGA AAGGCATGTG AGCTCCATGC CAGCACCCAG ACAGATGAAG 
GAAAACCAGG TGCCCACCAA AGGAACATGG AGGAAAATCA AGGAAAGTGA CATTTCTCCC 
ACAGGCATGG CTTCTCAGAG CGCTTCCTCA GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT 
CTGATCTATC AGATGGCACC TCCTGTCTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG 
GACTGCCCCA TTAACAACCC TAGATCTGGA CGGTCCCCCA CAGGCAACAC CCCCCCAGTG 
ATTGACAGTG TTTCAGAGAA GGGAAGTTCA AGCATTAAAG ATTCAAAAGA CACCCATGGG 
AAACAGAGTG TGGGCAGTGG CAGTCCTGTG CAAACCGTGG GTCTGGAAAC CCGCCTCAAC 
TCCTTTGTTC AGGTAGAGGC CCCAGAACAG AAAGGAACTG AGGCAAAACC AGGACAGAGT 
AACCCAGTCT CTATAGCAGA GACTGCTGAG ACGTGTATAG CAGAGCGTAC CCCTTTCAGT 
TCCAGTAGCT CCAGCAAGCA CAGCTCACCT AGCGGGACTG TTGCTGCCAG AGTGACACCT 
TTTAATTACA ACCCTAGCCC TAGGAAGAGC AGCGCAGACA GCACTTCAGC CCGGCCGTCT 
CAGATCCCTA CGCCAGTGAG CACCAACACG AAGAAGAGAG ATTCGAAGAC TGACAGCACA 
GAATCCAGTG GAGCCCAAAG TCCTAAACGC CATTCCGGGT CTTACCTCGT GACGTCTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347909.1
CDS	60..8642
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	NM_001360980.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360980.1

ORF Insert Sequence:

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGTAGGAAG GCTACCCGGG GCATTTCATC CCAGGAGCTA 
GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC ATTGCAGAGT TATTGCAGGT GGACTGTGAG 
ATGTATGGGC TTACTAATGA CCACTACAGT GTTACTTTAA GACGGTATGC TGGAATGGCT 
TTGACAAACT TGACCTTTGG AGATGTTGCC AACAAGGCTA CGCTGTGTTC TATGAAAGGC 
TGCATGAGAG CACTTGTGGC CCAGTTAAAA TCTGAGAGTG AAGACTTACA GCAGGTTATT 
GCAAGTGTTT TGAGGAATTT GTCTTGGCGA GCAGATGTAA ATAGCAAAAA GACGTTGAGA 
GAAGTTGGAA GTGTGAAAGC ATTGATGGAA TGTGCTTTGG AAGTTAAAAA GGAATCAACC 
CTCAAAAGCG TTTTGAGTGC CTTATGGAAC CTGTCTGCAC ACTGCACTGA GAATAAGGCT 
GACATCTGTG CTGTGGATGG AGCACTGGCA TTTCTGGTTG GCACCCTCAC TTACCGGAGC 
CAGACAAATA CTTTAGCCAT TATTGAAAGT GGAGGTGGGA TATTACGGAA TGTGTCCAGC 
TTGATAGCTA CAAACGAAGA CCACAGGCAA ATCCTAAGAG AGAACAATTG CCTACAAACT 
TTATTACAGC ACTTGAAATC TCACAGCTTG ACAATAGTCA GTAATGCATG TGGAACTTTG 
TGGAATCTCT CAGCAAGAAA TCCTAAAGAC CAGGAAGCCT TGTGGGACAT GGGGGCAGTG 
AGCATGCTCA AGAACCTCAT TCATTCCAAG CACAAAATGA TTGCCATGGG AAGTGCAGCA 
GCTTTAAGGA ATCTCATGGC AAACAGACCT GCAAAGTATA AGGATGCCAA TATCATGTCT 
CCCGGCTCAA GTCTGCCATC CCTTCACGTT AGGAAACAGA AAGCTCTAGA AGCTGAGCTA 
GATGCTCAGC ATTTATCAGA AACCTTCGAC AACATTGACA ACCTAAGTCC CAAGGCCTCT 
CACCGGAGTA AGCAGAGACA CAAGCAGAAT CTTTATGGTG ACTATGCTTT TGACGCCAAT 
CGACATGATG ATAGTAGGTC AGACAATTTC AATACTGGAA ACATGACTGT TCTTTCACCA 
TATTTAAATA CTACGGTATT GCCCAGCTCT TCTTCCTCAA GGGGAAGTTT AGACAGTTCT 
CGTTCTGAGA AAGACAGAAG TTTGGAGAGA GAGCGAGGTA TTGGCCTCAG TGCTTACCAT 
CCAACAACAG AAAATGCAGG AACCTCATCA AAACGAGGTC TGCAGATCAC TACCACTGCA 
GCCCAGATAG CCAAAGTTAT GGAAGAAGTA TCAGCCATTC ATACCTCCCA GGACGACAGA 
AGTTCTGCTT CTACCACCGA GTTCCATTGT GTGGCAGACG ACAGGAGTGC GGCACGAAGA 
AGCTCTGCCT CCCACACACA CTCAAACACA TACAACTTCA CTAAGTCGGA AAATTCAAAT 
AGGACATGCT CTATGCCTTA TGCCAAAGTG GAATATAAAC GATCTTCAAA TGACAGTTTA 
AATAGTGTCA CTAGTAGTGA TGGATATGGT AAAAGAGGCC AAATGAAACC CTCAGTTGAA 
TCCTATTCTG AAGATGATGA AAGTAAATTT TGCAGTTATG GTCAGTATCC AGCTGACCTA 
GCCCATAAGA TACACAGTGC AAATCATATG GATGATAATG ATGGAGAACT GGATACACCA 
ATAAATTACA GTCTTAAATA TTCAGATGAG CAGTTGAACT CAGGAAGGCA GAGTCCCTCA 
CAGAATGAAA GGTGGGCAAG ACCAAAGCAT GTGATAGAAG ATGAAATAAA GCAAAACGAG 
CAAAGACAAG CAAGAAGCCA GAACACCAGT TATCCTGTCT ATTCTGAGAA TACCGATGAC 
AAACACCTCA AATTCCAACC ACATTTTGGA CAACAAGAAT GTGTTTCCCC ATATAGGTCA 
AGGGGAACCA GTGGTTCAGA AACAAATCGA ATGGGTTCTA GTCATGCAAT TAATCAAAAT 
GTAAACCAGT CTCTGTGTCA GGAAGATGAT TATGAAGATG ATAAACCTAC CAACTACAGT 
GAACGTTATT CTGAGGAAGA ACAACATGAA GAAGAAGAAG AGAGACCGAC AAATTATAGC 
ATAAAATATA ATGAAGAGAA ACATCATGTG GATCAGCCTA TTGATTATAG TTTAAAATAT 
GCCACTGACA TTTCTTCCTC ACAAAAACCA TCATTTTCAT TCTCAAAGAA TTCATCAGCA 
CAAAGCACTA AACCTGAACA TCTCTCTCCA AGCAGCGAGA ATACAGCTGT ACCTCCATCT 
AATGCCAAAA GGCAGAATCA GCTGCGTCCA AGTTCAGCAC AAAGAAATGG CCAGACTCAA 
AAAGGCACTA CTTGCAAAGT CCCCTCCATC AACCAAGAAA CAATACAGAC TTACTGCGTA 
GAAGACACCC CAATATGTTT TTCAAGGTGC AGTTCATTAT CATCACTGTC ATCAGCTGAC 
GATGAAATAG GATGTGATCA GACAACACAG GAAGCAGATT CTGCTAATAC TCTGCAGACA 
GCAGAAGTAA AAGAGAATGA TGTAACTCGG TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA 
GCAGTGTCCC AGAATGCTAG AGCCAAACCC AGCCGACTCC AGGCTTCTGG CTTATCTTCA 
GAATCAACCA GGCATAATAA AGCTGTTGAG TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA 
AGTGGTGCTC AGACACCCAA AAGTCCCCCA GAACACTATG TCCAGGAGAC TCCGCTCGTA 
TTCAGCAGGT GTACTTCTGT CAGCTCCCTT GACAGTTTTG AGAGTCGCTC CATTGCCAGC 
TCTGTTCAGA GTGAGCCATG TAGTGGAATG GTGAGTGGCA TCATAAGCCC CAGTGACCTT 
CCAGATAGTC CTGGGCAGAC CATGCCACCA AGCAGAAGCA AAACCCCTCC ACCTCCTCCA 
CAGACAGTGC AGGCCAAGAG AGAGGTGCCA AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA 
GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA 
GACGTGGATA CTTTGTTACA CTTCGCCACA GAAAGTACTC CAGACGGGTT TTCTTGTTCC 
TCCAGCCTAA GTGCTCTGAG CCTGGATGAG CCATTTATAC AGAAAGATGT AGAATTAAGA 
ATCATGCCTC CAGTTCAGGA AAACGACAAT GGGAATGAAA CTGAATCAGA ACAGCCTGAG 
GAATCAAATG AAAACCAGGA TAAAGAGGTA GAAAAGCCTG ACTCTGAAAA AGACTTATTA 
GATGATTCTG ATGACGATGA TATTGAAATA TTAGAAGAAT GTATTATTTC AGCCATGCCA 
ACAAAGTCAT CACGCAAAGC CAAAAAACTA GCCCAGACTG CTTCAAAATT ACCTCCACCT 
GTGGCAAGGA AACCAAGTCA GCTACCTGTG TATAAACTTC TGCCAGCACA GAATAGGCTG 
CAGGCACAAA AACATGTTAG CTTTACACCA GGGGATGATG TGCCCCGGGT GTACTGTGTA 
GAAGGGACAC CTATAAACTT TTCCACAGCA ACGTCTCTAA GTGATCTGAC AATAGAGTCC 
CCTCCAAATG AATTGGCTAC TGGAGATGGG GTCAGAGCGG GTATACAGTC AGGTGAATTT 
GAAAAACGAG ATACCATTCC TACAGAAGGC AGAAGTACAG ATGATGCTCA GCGAGGAAAA 
ATCTCATCTA TAGTTACACC AGACCTGGAT GACAACAAAG CAGAGGAAGG AGATATTCTT 
GCAGAATGTA TCAATTCTGC TATGCCCAAA GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA 
AAGATAATGG ACCAAGTCCA ACAAGCATCC TCGACTTCAT CTGGAGCTAA CAAAAATCAA 
GTAGACACTA AGAAAAAGAA GCCTACTTCA CCAGTAAAGC CCATGCCACA AAATACTGAA 
TATAGAACGC GTGTGAGAAA GAATACAGAC TCAAAAGTTA ATGTAAATAC TGAAGAAACT 
TTCTCAGACA ACAAAGACTC AAAGAAACCA AGCTTACAAA CCAATGCCAA GGCCTTCAAT 
GAAAAGCTAC CTAACAATGA AGACAGAGTG CGGGGGAGCT TCGCCTTGGA CTCACCGCAT 
CACTACACCC CTATTGAGGG GACGCCGTAC TGCTTTTCCC GAAATGACTC CTTGAGTTCT 
CTGGATTTTG ATGATGACGA TGTTGACCTT TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC 
AAAGAAAGCA AGGATTCCGA AGCCAAAGTT ACCTGCCGCC CAGAACCAAA CTCAAGCCAG 
CAGGCAGCTA GTAAGTCACA AGCCAGTATA AAACATCCAG CAAACAGAGC ACAGTCCAAA 
CCAGTGCTGC AGAAACAGCC CACTTTCCCC CAGTCCTCCA AAGACGGACC AGATAGAGGG 
GCAGCAACTG ACGAAAAACT GCAGAATTTT GCTATTGAAA ATACTCCAGT TTGCTTTTCT 
CGAAATTCCT CTCTGAGTTC CCTTAGTGAC ATTGACCAGG AAAACAACAA TAACAAAGAA 
AGTGAACCAA TCAAAGAAGC TGAACCTGCC AACTCACAAG GAGAGCCCAG TAAGCCTCAG 
GCATCCGGGT ATGCTCCCAA GTCCTTCCAC GTCGAAGACA CCCCTGTCTG TTTCTCAAGA 
AACAGCTCTC TCAGTTCTCT TAGCATTGAC TCTGAGGACG ACCTGTTACA GGAGTGTATA 
AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT TCAAGACTCA AGAGTGAGAG CGAAAAGCAG 
AGCCCTAGAA AAGTGGGTGG CATATTAGCT GAAGACCTGA CGCTTGATTT GAAAGATCTA 
CAGAGGCCAG ATTCAGAACA CGCTTTCTCC CCCGACTCAG AAAATTTTGA CTGGAAAGCT 
ATTCAGGAAG GCGCAAACTC CATAGTAAGT AGTTTGCACC AAGCTGCTGC AGCCGCCGCG 
TGCTTATCTA GACAAGCGTC ATCCGACTCA GATTCCATTC TGTCACTAAA GTCCGGCATT 
TCTCTGGGAT CGCCTTTTCA TCTTACACCT GATCAAGAGG AAAAGCCATT CACAAGCAAT 
AAAGGCCCAA GAATTCTCAA ACCTGGAGAG AAAAGCACAT TAGAAGCAAA AAAAATAGAA 
TCTGAAAACA AAGGAATCAA AGGCGGGAAA AAGGTTTATA AAAGCTTGAT TACGGGAAAG 
ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA ATGAAACAAC CCCTCCCGAC AAACATGCCT 
TCAATCTCAA GAGGCAGGAC GATGATTCAC ATCCCAGGGC TTCGGAATAG CTCCTCTAGT 
ACAAGCCCTG TCTCTAAGAA AGGCCCACCC CTCAAGACTC CAGCCTCTAA AAGCCCCAGT 
GAAGGGCCGG GAGCTACCAC TTCTCCTCGA GGAACTAAGC CAGCAGGAAA GTCAGAGCTT 
AGCCCTATCA CCAGGCAAAC TTCCCAAATC AGTGGGTCAA ATAAGGGGTC TTCTAGATCA 
GGATCTAGAG ACTCCACTCC CTCAAGACCT ACACAGCAAC CATTAAGTAG GCCAATGCAG 
TCTCCAGGGC GAAACTCAAT TTCCCCTGGT AGAAATGGAA TAAGCCCTCC TAACAAACTG 
TCTCAGCTGC CCAGAACATC ATCTCCCAGT ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG 
AAAATGTCAT ATACATCCCC AGGTAGACAG CTGAGCCAAC AAAATCTTAC CAAACAAGCA 
AGTTTATCCA AGAATGCCAG CAGTATCCCC AGAAGTGAGT CGGCATCTAA AGGACTGAAT 
CAGATGAGTA ACGGCAATGG GTCAAATAAA AAGGTAGAAC TTTCTAGAAT GTCTTCAACT 
AAATCAAGTG GAAGTGAATC AGACAGATCA GAAAGGCCTG CATTAGTACG CCAGTCTACT 
TTCATCAAAG AAGCCCCAAG CCCAACCCTG AGGAGGAAAC TGGAGGAATC TGCCTCATTT 
GAATCCCTTT CTCCATCTTC TAGACCAGAT TCTCCCACCA GGTCGCAGGC ACAGACCCCA 
GTTTTAAGCC CTTCCCTTCC TGATATGTCT CTGTCCACAC ATCCATCTGT TCAGGCAGGT 
GGGTGGCGAA AGCTCCCGCC TAATCTCAGC CCCACTATCG AGTATAATGA CGGAAGGCCC 
ACAAAACGGC ATGATATTGC ACGCTCCCAT TCTGAAAGTC CTTCCAGACT ACCAATCAAC 
CGGGCGGGAA CCTGGAAGCG TGAACACAGC AAACATTCCT CGTCCCTTCC TCGAGTGAGT 
ACTTGGAGAA GAACTGGAAG CTCATCTTCT ATTCTTTCTG CTTCATCAGA GTCCAGTGAA 
AAAGCAAAAA GTGAGGATGA AAGGCATGTG AGCTCCATGC CAGCACCCAG ACAGATGAAG 
GAAAACCAGG TGCCCACCAA AGGAACATGG AGGAAAATCA AGGAAAGTGA CATTTCTCCC 
ACAGGCATGG CTTCTCAGAG CGCTTCCTCA GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT 
CTGATCTATC AGATGGCACC TCCTGTCTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG 
GACTGCCCCA TTAACAACCC TAGATCTGGA CGGTCCCCCA CAGGCAACAC CCCCCCAGTG 
ATTGACAGTG TTTCAGAGAA GGGAAGTTCA AGCATTAAAG ATTCAAAAGA CACCCATGGG 
AAACAGAGTG TGGGCAGTGG CAGTCCTGTG CAAACCGTGG GTCTGGAAAC CCGCCTCAAC 
TCCTTTGTTC AGGTAGAGGC CCCAGAACAG AAAGGAACTG AGGCAAAACC AGGACAGAGT 
AACCCAGTCT CTATAGCAGA GACTGCTGAG ACGTGTATAG CAGAGCGTAC CCCTTTCAGT 
TCCAGTAGCT CCAGCAAGCA CAGCTCACCT AGCGGGACTG TTGCTGCCAG AGTGACACCT 
TTTAATTACA ACCCTAGCCC TAGGAAGAGC AGCGCAGACA GCACTTCAGC CCGGCCGTCT 
CAGATCCCTA CGCCAGTGAG CACCAACACG AAGAAGAGAG ATTCGAAGAC TGACAGCACA 
GAATCCAGTG GAGCCCAAAG TCCTAAACGC CATTCCGGGT CTTACCTCGT GACGTCTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( NM_001360979.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347908.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant 2, mRNA.

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CloneID	OMu79754
Clone ID Related Accession (Same CDS sequence)	XM_030250287.1 , XM_030250286.1 , NM_001360979.1 , XM_017317804.1
Accession Version	NM_001360979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576425600000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114003.4, AL591373.8 and AC090534.10. On Feb 23, 2018 this sequence version replaced XM_017317804.1. Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.131217.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGGGGA CTGCAGGCCA TTGCAGAGTT ATTGCAGGTG 
GACTGTGAGA TGTATGGGCT TACTAATGAC CACTACAGTG TTACTTTAAG ACGGTATGCT 
GGAATGGCTT TGACAAACTT GACCTTTGGA GATGTTGCCA ACAAGGCTAC GCTGTGTTCT 
ATGAAAGGCT GCATGAGAGC ACTTGTGGCC CAGTTAAAAT CTGAGAGTGA AGACTTACAG 
CAGGTTATTG CAAGTGTTTT GAGGAATTTG TCTTGGCGAG CAGATGTAAA TAGCAAAAAG 
ACGTTGAGAG AAGTTGGAAG TGTGAAAGCA TTGATGGAAT GTGCTTTGGA AGTTAAAAAG 
GAATCAACCC TCAAAAGCGT TTTGAGTGCC TTATGGAACC TGTCTGCACA CTGCACTGAG 
AATAAGGCTG ACATCTGTGC TGTGGATGGA GCACTGGCAT TTCTGGTTGG CACCCTCACT 
TACCGGAGCC AGACAAATAC TTTAGCCATT ATTGAAAGTG GAGGTGGGAT ATTACGGAAT 
GTGTCCAGCT TGATAGCTAC AAACGAAGAC CACAGGCAAA TCCTAAGAGA GAACAATTGC 
CTACAAACTT TATTACAGCA CTTGAAATCT CACAGCTTGA CAATAGTCAG TAATGCATGT 
GGAACTTTGT GGAATCTCTC AGCAAGAAAT CCTAAAGACC AGGAAGCCTT GTGGGACATG 
GGGGCAGTGA GCATGCTCAA GAACCTCATT CATTCCAAGC ACAAAATGAT TGCCATGGGA 
AGTGCAGCAG CTTTAAGGAA TCTCATGGCA AACAGACCTG CAAAGTATAA GGATGCCAAT 
ATCATGTCTC CCGGCTCAAG TCTGCCATCC CTTCACGTTA GGAAACAGAA AGCTCTAGAA 
GCTGAGCTAG ATGCTCAGCA TTTATCAGAA ACCTTCGACA ACATTGACAA CCTAAGTCCC 
AAGGCCTCTC ACCGGAGTAA GCAGAGACAC AAGCAGAATC TTTATGGTGA CTATGCTTTT 
GACGCCAATC GACATGATGA TAGTAGGTCA GACAATTTCA ATACTGGAAA CATGACTGTT 
CTTTCACCAT ATTTAAATAC TACGGTATTG CCCAGCTCTT CTTCCTCAAG GGGAAGTTTA 
GACAGTTCTC GTTCTGAGAA AGACAGAAGT TTGGAGAGAG AGCGAGGTAT TGGCCTCAGT 
GCTTACCATC CAACAACAGA AAATGCAGGA ACCTCATCAA AACGAGGTCT GCAGATCACT 
ACCACTGCAG CCCAGATAGC CAAAGTTATG GAAGAAGTAT CAGCCATTCA TACCTCCCAG 
GACGACAGAA GTTCTGCTTC TACCACCGAG TTCCATTGTG TGGCAGACGA CAGGAGTGCG 
GCACGAAGAA GCTCTGCCTC CCACACACAC TCAAACACAT ACAACTTCAC TAAGTCGGAA 
AATTCAAATA GGACATGCTC TATGCCTTAT GCCAAAGTGG AATATAAACG ATCTTCAAAT 
GACAGTTTAA ATAGTGTCAC TAGTAGTGAT GGATATGGTA AAAGAGGCCA AATGAAACCC 
TCAGTTGAAT CCTATTCTGA AGATGATGAA AGTAAATTTT GCAGTTATGG TCAGTATCCA 
GCTGACCTAG CCCATAAGAT ACACAGTGCA AATCATATGG ATGATAATGA TGGAGAACTG 
GATACACCAA TAAATTACAG TCTTAAATAT TCAGATGAGC AGTTGAACTC AGGAAGGCAG 
AGTCCCTCAC AGAATGAAAG GTGGGCAAGA CCAAAGCATG TGATAGAAGA TGAAATAAAG 
CAAAACGAGC AAAGACAAGC AAGAAGCCAG AACACCAGTT ATCCTGTCTA TTCTGAGAAT 
ACCGATGACA AACACCTCAA ATTCCAACCA CATTTTGGAC AACAAGAATG TGTTTCCCCA 
TATAGGTCAA GGGGAACCAG TGGTTCAGAA ACAAATCGAA TGGGTTCTAG TCATGCAATT 
AATCAAAATG TAAACCAGTC TCTGTGTCAG GAAGATGATT ATGAAGATGA TAAACCTACC 
AACTACAGTG AACGTTATTC TGAGGAAGAA CAACATGAAG AAGAAGAAGA GAGACCGACA 
AATTATAGCA TAAAATATAA TGAAGAGAAA CATCATGTGG ATCAGCCTAT TGATTATAGT 
TTAAAATATG CCACTGACAT TTCTTCCTCA CAAAAACCAT CATTTTCATT CTCAAAGAAT 
TCATCAGCAC AAAGCACTAA ACCTGAACAT CTCTCTCCAA GCAGCGAGAA TACAGCTGTA 
CCTCCATCTA ATGCCAAAAG GCAGAATCAG CTGCGTCCAA GTTCAGCACA AAGAAATGGC 
CAGACTCAAA AAGGCACTAC TTGCAAAGTC CCCTCCATCA ACCAAGAAAC AATACAGACT 
TACTGCGTAG AAGACACCCC AATATGTTTT TCAAGGTGCA GTTCATTATC ATCACTGTCA 
TCAGCTGACG ATGAAATAGG ATGTGATCAG ACAACACAGG AAGCAGATTC TGCTAATACT 
CTGCAGACAG CAGAAGTAAA AGAGAATGAT GTAACTCGGT CAGCTGAAGA TCCTGCAACT 
GAAGTTCCAG CAGTGTCCCA GAATGCTAGA GCCAAACCCA GCCGACTCCA GGCTTCTGGC 
TTATCTTCAG AATCAACCAG GCATAATAAA GCTGTTGAGT TTTCTTCAGG AGCCAAGTCT 
CCCTCCAAAA GTGGTGCTCA GACACCCAAA AGTCCCCCAG AACACTATGT CCAGGAGACT 
CCGCTCGTAT TCAGCAGGTG TACTTCTGTC AGCTCCCTTG ACAGTTTTGA GAGTCGCTCC 
ATTGCCAGCT CTGTTCAGAG TGAGCCATGT AGTGGAATGG TGAGTGGCAT CATAAGCCCC 
AGTGACCTTC CAGATAGTCC TGGGCAGACC ATGCCACCAA GCAGAAGCAA AACCCCTCCA 
CCTCCTCCAC AGACAGTGCA GGCCAAGAGA GAGGTGCCAA AAAGTAAAGT CCCTGCTGCT 
GAGAAGAGAG AGAGTGGGCC TAAGCAGACT GCTGTAAATG CTGCCGTGCA GAGGGTGCAG 
GTCCTTCCAG ACGTGGATAC TTTGTTACAC TTCGCCACAG AAAGTACTCC AGACGGGTTT 
TCTTGTTCCT CCAGCCTAAG TGCTCTGAGC CTGGATGAGC CATTTATACA GAAAGATGTA 
GAATTAAGAA TCATGCCTCC AGTTCAGGAA AACGACAATG GGAATGAAAC TGAATCAGAA 
CAGCCTGAGG AATCAAATGA AAACCAGGAT AAAGAGGTAG AAAAGCCTGA CTCTGAAAAA 
GACTTATTAG ATGATTCTGA TGACGATGAT ATTGAAATAT TAGAAGAATG TATTATTTCA 
GCCATGCCAA CAAAGTCATC ACGCAAAGCC AAAAAACTAG CCCAGACTGC TTCAAAATTA 
CCTCCACCTG TGGCAAGGAA ACCAAGTCAG CTACCTGTGT ATAAACTTCT GCCAGCACAG 
AATAGGCTGC AGGCACAAAA ACATGTTAGC TTTACACCAG GGGATGATGT GCCCCGGGTG 
TACTGTGTAG AAGGGACACC TATAAACTTT TCCACAGCAA CGTCTCTAAG TGATCTGACA 
ATAGAGTCCC CTCCAAATGA ATTGGCTACT GGAGATGGGG TCAGAGCGGG TATACAGTCA 
GGTGAATTTG AAAAACGAGA TACCATTCCT ACAGAAGGCA GAAGTACAGA TGATGCTCAG 
CGAGGAAAAA TCTCATCTAT AGTTACACCA GACCTGGATG ACAACAAAGC AGAGGAAGGA 
GATATTCTTG CAGAATGTAT CAATTCTGCT ATGCCCAAAG GAAAAAGCCA CAAGCCTTTC 
CGAGTGAAAA AGATAATGGA CCAAGTCCAA CAAGCATCCT CGACTTCATC TGGAGCTAAC 
AAAAATCAAG TAGACACTAA GAAAAAGAAG CCTACTTCAC CAGTAAAGCC CATGCCACAA 
AATACTGAAT ATAGAACGCG TGTGAGAAAG AATACAGACT CAAAAGTTAA TGTAAATACT 
GAAGAAACTT TCTCAGACAA CAAAGACTCA AAGAAACCAA GCTTACAAAC CAATGCCAAG 
GCCTTCAATG AAAAGCTACC TAACAATGAA GACAGAGTGC GGGGGAGCTT CGCCTTGGAC 
TCACCGCATC ACTACACCCC TATTGAGGGG ACGCCGTACT GCTTTTCCCG AAATGACTCC 
TTGAGTTCTC TGGATTTTGA TGATGACGAT GTTGACCTTT CCAGGGAAAA GGCCGAGTTA 
AGAAAGGGCA AAGAAAGCAA GGATTCCGAA GCCAAAGTTA CCTGCCGCCC AGAACCAAAC 
TCAAGCCAGC AGGCAGCTAG TAAGTCACAA GCCAGTATAA AACATCCAGC AAACAGAGCA 
CAGTCCAAAC CAGTGCTGCA GAAACAGCCC ACTTTCCCCC AGTCCTCCAA AGACGGACCA 
GATAGAGGGG CAGCAACTGA CGAAAAACTG CAGAATTTTG CTATTGAAAA TACTCCAGTT 
TGCTTTTCTC GAAATTCCTC TCTGAGTTCC CTTAGTGACA TTGACCAGGA AAACAACAAT 
AACAAAGAAA GTGAACCAAT CAAAGAAGCT GAACCTGCCA ACTCACAAGG AGAGCCCAGT 
AAGCCTCAGG CATCCGGGTA TGCTCCCAAG TCCTTCCACG TCGAAGACAC CCCTGTCTGT 
TTCTCAAGAA ACAGCTCTCT CAGTTCTCTT AGCATTGACT CTGAGGACGA CCTGTTACAG 
GAGTGTATAA GTTCTGCCAT GCCAAAAAAG AAAAGGCCTT CAAGACTCAA GAGTGAGAGC 
GAAAAGCAGA GCCCTAGAAA AGTGGGTGGC ATATTAGCTG AAGACCTGAC GCTTGATTTG 
AAAGATCTAC AGAGGCCAGA TTCAGAACAC GCTTTCTCCC CCGACTCAGA AAATTTTGAC 
TGGAAAGCTA TTCAGGAAGG CGCAAACTCC ATAGTAAGTA GTTTGCACCA AGCTGCTGCA 
GCCGCCGCGT GCTTATCTAG ACAAGCGTCA TCCGACTCAG ATTCCATTCT GTCACTAAAG 
TCCGGCATTT CTCTGGGATC GCCTTTTCAT CTTACACCTG ATCAAGAGGA AAAGCCATTC 
ACAAGCAATA AAGGCCCAAG AATTCTCAAA CCTGGAGAGA AAAGCACATT AGAAGCAAAA 
AAAATAGAAT CTGAAAACAA AGGAATCAAA GGCGGGAAAA AGGTTTATAA AAGCTTGATT 
ACGGGAAAGA TTCGCTCCAA TTCAGAAATT TCCAGCCAAA TGAAACAACC CCTCCCGACA 
AACATGCCTT CAATCTCAAG AGGCAGGACG ATGATTCACA TCCCAGGGCT TCGGAATAGC 
TCCTCTAGTA CAAGCCCTGT CTCTAAGAAA GGCCCACCCC TCAAGACTCC AGCCTCTAAA 
AGCCCCAGTG AAGGGCCGGG AGCTACCACT TCTCCTCGAG GAACTAAGCC AGCAGGAAAG 
TCAGAGCTTA GCCCTATCAC CAGGCAAACT TCCCAAATCA GTGGGTCAAA TAAGGGGTCT 
TCTAGATCAG GATCTAGAGA CTCCACTCCC TCAAGACCTA CACAGCAACC ATTAAGTAGG 
CCAATGCAGT CTCCAGGGCG AAACTCAATT TCCCCTGGTA GAAATGGAAT AAGCCCTCCT 
AACAAACTGT CTCAGCTGCC CAGAACATCA TCTCCCAGTA CTGCTTCAAC TAAGTCCTCC 
GGTTCTGGGA AAATGTCATA TACATCCCCA GGTAGACAGC TGAGCCAACA AAATCTTACC 
AAACAAGCAA GTTTATCCAA GAATGCCAGC AGTATCCCCA GAAGTGAGTC GGCATCTAAA 
GGACTGAATC AGATGAGTAA CGGCAATGGG TCAAATAAAA AGGTAGAACT TTCTAGAATG 
TCTTCAACTA AATCAAGTGG AAGTGAATCA GACAGATCAG AAAGGCCTGC ATTAGTACGC 
CAGTCTACTT TCATCAAAGA AGCCCCAAGC CCAACCCTGA GGAGGAAACT GGAGGAATCT 
GCCTCATTTG AATCCCTTTC TCCATCTTCT AGACCAGATT CTCCCACCAG GTCGCAGGCA 
CAGACCCCAG TTTTAAGCCC TTCCCTTCCT GATATGTCTC TGTCCACACA TCCATCTGTT 
CAGGCAGGTG GGTGGCGAAA GCTCCCGCCT AATCTCAGCC CCACTATCGA GTATAATGAC 
GGAAGGCCCA CAAAACGGCA TGATATTGCA CGCTCCCATT CTGAAAGTCC TTCCAGACTA 
CCAATCAACC GGGCGGGAAC CTGGAAGCGT GAACACAGCA AACATTCCTC GTCCCTTCCT 
CGAGTGAGTA CTTGGAGAAG AACTGGAAGC TCATCTTCTA TTCTTTCTGC TTCATCAGAG 
TCCAGTGAAA AAGCAAAAAG TGAGGATGAA AGGCATGTGA GCTCCATGCC AGCACCCAGA 
CAGATGAAGG AAAACCAGGT GCCCACCAAA GGAACATGGA GGAAAATCAA GGAAAGTGAC 
ATTTCTCCCA CAGGCATGGC TTCTCAGAGC GCTTCCTCAG GTGCTGCCAG TGGTGCTGAA 
TCCAAGCCTC TGATCTATCA GATGGCACCT CCTGTCTCTA AAACAGAGGA TGTTTGGGTG 
AGAATTGAGG ACTGCCCCAT TAACAACCCT AGATCTGGAC GGTCCCCCAC AGGCAACACC 
CCCCCAGTGA TTGACAGTGT TTCAGAGAAG GGAAGTTCAA GCATTAAAGA TTCAAAAGAC 
ACCCATGGGA AACAGAGTGT GGGCAGTGGC AGTCCTGTGC AAACCGTGGG TCTGGAAACC 
CGCCTCAACT CCTTTGTTCA GGTAGAGGCC CCAGAACAGA AAGGAACTGA GGCAAAACCA 
GGACAGAGTA ACCCAGTCTC TATAGCAGAG ACTGCTGAGA CGTGTATAGC AGAGCGTACC 
CCTTTCAGTT CCAGTAGCTC CAGCAAGCAC AGCTCACCTA GCGGGACTGT TGCTGCCAGA 
GTGACACCTT TTAATTACAA CCCTAGCCCT AGGAAGAGCA GCGCAGACAG CACTTCAGCC 
CGGCCGTCTC AGATCCCTAC GCCAGTGAGC ACCAACACGA AGAAGAGAGA TTCGAAGACT 
GACAGCACAG AATCCAGTGG AGCCCAAAGT CCTAAACGCC ATTCCGGGTC TTACCTCGTG 
ACGTCTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347908.1
CDS	249..8600
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	NM_001360979.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360979.1

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGGGGA CTGCAGGCCA TTGCAGAGTT ATTGCAGGTG 
GACTGTGAGA TGTATGGGCT TACTAATGAC CACTACAGTG TTACTTTAAG ACGGTATGCT 
GGAATGGCTT TGACAAACTT GACCTTTGGA GATGTTGCCA ACAAGGCTAC GCTGTGTTCT 
ATGAAAGGCT GCATGAGAGC ACTTGTGGCC CAGTTAAAAT CTGAGAGTGA AGACTTACAG 
CAGGTTATTG CAAGTGTTTT GAGGAATTTG TCTTGGCGAG CAGATGTAAA TAGCAAAAAG 
ACGTTGAGAG AAGTTGGAAG TGTGAAAGCA TTGATGGAAT GTGCTTTGGA AGTTAAAAAG 
GAATCAACCC TCAAAAGCGT TTTGAGTGCC TTATGGAACC TGTCTGCACA CTGCACTGAG 
AATAAGGCTG ACATCTGTGC TGTGGATGGA GCACTGGCAT TTCTGGTTGG CACCCTCACT 
TACCGGAGCC AGACAAATAC TTTAGCCATT ATTGAAAGTG GAGGTGGGAT ATTACGGAAT 
GTGTCCAGCT TGATAGCTAC AAACGAAGAC CACAGGCAAA TCCTAAGAGA GAACAATTGC 
CTACAAACTT TATTACAGCA CTTGAAATCT CACAGCTTGA CAATAGTCAG TAATGCATGT 
GGAACTTTGT GGAATCTCTC AGCAAGAAAT CCTAAAGACC AGGAAGCCTT GTGGGACATG 
GGGGCAGTGA GCATGCTCAA GAACCTCATT CATTCCAAGC ACAAAATGAT TGCCATGGGA 
AGTGCAGCAG CTTTAAGGAA TCTCATGGCA AACAGACCTG CAAAGTATAA GGATGCCAAT 
ATCATGTCTC CCGGCTCAAG TCTGCCATCC CTTCACGTTA GGAAACAGAA AGCTCTAGAA 
GCTGAGCTAG ATGCTCAGCA TTTATCAGAA ACCTTCGACA ACATTGACAA CCTAAGTCCC 
AAGGCCTCTC ACCGGAGTAA GCAGAGACAC AAGCAGAATC TTTATGGTGA CTATGCTTTT 
GACGCCAATC GACATGATGA TAGTAGGTCA GACAATTTCA ATACTGGAAA CATGACTGTT 
CTTTCACCAT ATTTAAATAC TACGGTATTG CCCAGCTCTT CTTCCTCAAG GGGAAGTTTA 
GACAGTTCTC GTTCTGAGAA AGACAGAAGT TTGGAGAGAG AGCGAGGTAT TGGCCTCAGT 
GCTTACCATC CAACAACAGA AAATGCAGGA ACCTCATCAA AACGAGGTCT GCAGATCACT 
ACCACTGCAG CCCAGATAGC CAAAGTTATG GAAGAAGTAT CAGCCATTCA TACCTCCCAG 
GACGACAGAA GTTCTGCTTC TACCACCGAG TTCCATTGTG TGGCAGACGA CAGGAGTGCG 
GCACGAAGAA GCTCTGCCTC CCACACACAC TCAAACACAT ACAACTTCAC TAAGTCGGAA 
AATTCAAATA GGACATGCTC TATGCCTTAT GCCAAAGTGG AATATAAACG ATCTTCAAAT 
GACAGTTTAA ATAGTGTCAC TAGTAGTGAT GGATATGGTA AAAGAGGCCA AATGAAACCC 
TCAGTTGAAT CCTATTCTGA AGATGATGAA AGTAAATTTT GCAGTTATGG TCAGTATCCA 
GCTGACCTAG CCCATAAGAT ACACAGTGCA AATCATATGG ATGATAATGA TGGAGAACTG 
GATACACCAA TAAATTACAG TCTTAAATAT TCAGATGAGC AGTTGAACTC AGGAAGGCAG 
AGTCCCTCAC AGAATGAAAG GTGGGCAAGA CCAAAGCATG TGATAGAAGA TGAAATAAAG 
CAAAACGAGC AAAGACAAGC AAGAAGCCAG AACACCAGTT ATCCTGTCTA TTCTGAGAAT 
ACCGATGACA AACACCTCAA ATTCCAACCA CATTTTGGAC AACAAGAATG TGTTTCCCCA 
TATAGGTCAA GGGGAACCAG TGGTTCAGAA ACAAATCGAA TGGGTTCTAG TCATGCAATT 
AATCAAAATG TAAACCAGTC TCTGTGTCAG GAAGATGATT ATGAAGATGA TAAACCTACC 
AACTACAGTG AACGTTATTC TGAGGAAGAA CAACATGAAG AAGAAGAAGA GAGACCGACA 
AATTATAGCA TAAAATATAA TGAAGAGAAA CATCATGTGG ATCAGCCTAT TGATTATAGT 
TTAAAATATG CCACTGACAT TTCTTCCTCA CAAAAACCAT CATTTTCATT CTCAAAGAAT 
TCATCAGCAC AAAGCACTAA ACCTGAACAT CTCTCTCCAA GCAGCGAGAA TACAGCTGTA 
CCTCCATCTA ATGCCAAAAG GCAGAATCAG CTGCGTCCAA GTTCAGCACA AAGAAATGGC 
CAGACTCAAA AAGGCACTAC TTGCAAAGTC CCCTCCATCA ACCAAGAAAC AATACAGACT 
TACTGCGTAG AAGACACCCC AATATGTTTT TCAAGGTGCA GTTCATTATC ATCACTGTCA 
TCAGCTGACG ATGAAATAGG ATGTGATCAG ACAACACAGG AAGCAGATTC TGCTAATACT 
CTGCAGACAG CAGAAGTAAA AGAGAATGAT GTAACTCGGT CAGCTGAAGA TCCTGCAACT 
GAAGTTCCAG CAGTGTCCCA GAATGCTAGA GCCAAACCCA GCCGACTCCA GGCTTCTGGC 
TTATCTTCAG AATCAACCAG GCATAATAAA GCTGTTGAGT TTTCTTCAGG AGCCAAGTCT 
CCCTCCAAAA GTGGTGCTCA GACACCCAAA AGTCCCCCAG AACACTATGT CCAGGAGACT 
CCGCTCGTAT TCAGCAGGTG TACTTCTGTC AGCTCCCTTG ACAGTTTTGA GAGTCGCTCC 
ATTGCCAGCT CTGTTCAGAG TGAGCCATGT AGTGGAATGG TGAGTGGCAT CATAAGCCCC 
AGTGACCTTC CAGATAGTCC TGGGCAGACC ATGCCACCAA GCAGAAGCAA AACCCCTCCA 
CCTCCTCCAC AGACAGTGCA GGCCAAGAGA GAGGTGCCAA AAAGTAAAGT CCCTGCTGCT 
GAGAAGAGAG AGAGTGGGCC TAAGCAGACT GCTGTAAATG CTGCCGTGCA GAGGGTGCAG 
GTCCTTCCAG ACGTGGATAC TTTGTTACAC TTCGCCACAG AAAGTACTCC AGACGGGTTT 
TCTTGTTCCT CCAGCCTAAG TGCTCTGAGC CTGGATGAGC CATTTATACA GAAAGATGTA 
GAATTAAGAA TCATGCCTCC AGTTCAGGAA AACGACAATG GGAATGAAAC TGAATCAGAA 
CAGCCTGAGG AATCAAATGA AAACCAGGAT AAAGAGGTAG AAAAGCCTGA CTCTGAAAAA 
GACTTATTAG ATGATTCTGA TGACGATGAT ATTGAAATAT TAGAAGAATG TATTATTTCA 
GCCATGCCAA CAAAGTCATC ACGCAAAGCC AAAAAACTAG CCCAGACTGC TTCAAAATTA 
CCTCCACCTG TGGCAAGGAA ACCAAGTCAG CTACCTGTGT ATAAACTTCT GCCAGCACAG 
AATAGGCTGC AGGCACAAAA ACATGTTAGC TTTACACCAG GGGATGATGT GCCCCGGGTG 
TACTGTGTAG AAGGGACACC TATAAACTTT TCCACAGCAA CGTCTCTAAG TGATCTGACA 
ATAGAGTCCC CTCCAAATGA ATTGGCTACT GGAGATGGGG TCAGAGCGGG TATACAGTCA 
GGTGAATTTG AAAAACGAGA TACCATTCCT ACAGAAGGCA GAAGTACAGA TGATGCTCAG 
CGAGGAAAAA TCTCATCTAT AGTTACACCA GACCTGGATG ACAACAAAGC AGAGGAAGGA 
GATATTCTTG CAGAATGTAT CAATTCTGCT ATGCCCAAAG GAAAAAGCCA CAAGCCTTTC 
CGAGTGAAAA AGATAATGGA CCAAGTCCAA CAAGCATCCT CGACTTCATC TGGAGCTAAC 
AAAAATCAAG TAGACACTAA GAAAAAGAAG CCTACTTCAC CAGTAAAGCC CATGCCACAA 
AATACTGAAT ATAGAACGCG TGTGAGAAAG AATACAGACT CAAAAGTTAA TGTAAATACT 
GAAGAAACTT TCTCAGACAA CAAAGACTCA AAGAAACCAA GCTTACAAAC CAATGCCAAG 
GCCTTCAATG AAAAGCTACC TAACAATGAA GACAGAGTGC GGGGGAGCTT CGCCTTGGAC 
TCACCGCATC ACTACACCCC TATTGAGGGG ACGCCGTACT GCTTTTCCCG AAATGACTCC 
TTGAGTTCTC TGGATTTTGA TGATGACGAT GTTGACCTTT CCAGGGAAAA GGCCGAGTTA 
AGAAAGGGCA AAGAAAGCAA GGATTCCGAA GCCAAAGTTA CCTGCCGCCC AGAACCAAAC 
TCAAGCCAGC AGGCAGCTAG TAAGTCACAA GCCAGTATAA AACATCCAGC AAACAGAGCA 
CAGTCCAAAC CAGTGCTGCA GAAACAGCCC ACTTTCCCCC AGTCCTCCAA AGACGGACCA 
GATAGAGGGG CAGCAACTGA CGAAAAACTG CAGAATTTTG CTATTGAAAA TACTCCAGTT 
TGCTTTTCTC GAAATTCCTC TCTGAGTTCC CTTAGTGACA TTGACCAGGA AAACAACAAT 
AACAAAGAAA GTGAACCAAT CAAAGAAGCT GAACCTGCCA ACTCACAAGG AGAGCCCAGT 
AAGCCTCAGG CATCCGGGTA TGCTCCCAAG TCCTTCCACG TCGAAGACAC CCCTGTCTGT 
TTCTCAAGAA ACAGCTCTCT CAGTTCTCTT AGCATTGACT CTGAGGACGA CCTGTTACAG 
GAGTGTATAA GTTCTGCCAT GCCAAAAAAG AAAAGGCCTT CAAGACTCAA GAGTGAGAGC 
GAAAAGCAGA GCCCTAGAAA AGTGGGTGGC ATATTAGCTG AAGACCTGAC GCTTGATTTG 
AAAGATCTAC AGAGGCCAGA TTCAGAACAC GCTTTCTCCC CCGACTCAGA AAATTTTGAC 
TGGAAAGCTA TTCAGGAAGG CGCAAACTCC ATAGTAAGTA GTTTGCACCA AGCTGCTGCA 
GCCGCCGCGT GCTTATCTAG ACAAGCGTCA TCCGACTCAG ATTCCATTCT GTCACTAAAG 
TCCGGCATTT CTCTGGGATC GCCTTTTCAT CTTACACCTG ATCAAGAGGA AAAGCCATTC 
ACAAGCAATA AAGGCCCAAG AATTCTCAAA CCTGGAGAGA AAAGCACATT AGAAGCAAAA 
AAAATAGAAT CTGAAAACAA AGGAATCAAA GGCGGGAAAA AGGTTTATAA AAGCTTGATT 
ACGGGAAAGA TTCGCTCCAA TTCAGAAATT TCCAGCCAAA TGAAACAACC CCTCCCGACA 
AACATGCCTT CAATCTCAAG AGGCAGGACG ATGATTCACA TCCCAGGGCT TCGGAATAGC 
TCCTCTAGTA CAAGCCCTGT CTCTAAGAAA GGCCCACCCC TCAAGACTCC AGCCTCTAAA 
AGCCCCAGTG AAGGGCCGGG AGCTACCACT TCTCCTCGAG GAACTAAGCC AGCAGGAAAG 
TCAGAGCTTA GCCCTATCAC CAGGCAAACT TCCCAAATCA GTGGGTCAAA TAAGGGGTCT 
TCTAGATCAG GATCTAGAGA CTCCACTCCC TCAAGACCTA CACAGCAACC ATTAAGTAGG 
CCAATGCAGT CTCCAGGGCG AAACTCAATT TCCCCTGGTA GAAATGGAAT AAGCCCTCCT 
AACAAACTGT CTCAGCTGCC CAGAACATCA TCTCCCAGTA CTGCTTCAAC TAAGTCCTCC 
GGTTCTGGGA AAATGTCATA TACATCCCCA GGTAGACAGC TGAGCCAACA AAATCTTACC 
AAACAAGCAA GTTTATCCAA GAATGCCAGC AGTATCCCCA GAAGTGAGTC GGCATCTAAA 
GGACTGAATC AGATGAGTAA CGGCAATGGG TCAAATAAAA AGGTAGAACT TTCTAGAATG 
TCTTCAACTA AATCAAGTGG AAGTGAATCA GACAGATCAG AAAGGCCTGC ATTAGTACGC 
CAGTCTACTT TCATCAAAGA AGCCCCAAGC CCAACCCTGA GGAGGAAACT GGAGGAATCT 
GCCTCATTTG AATCCCTTTC TCCATCTTCT AGACCAGATT CTCCCACCAG GTCGCAGGCA 
CAGACCCCAG TTTTAAGCCC TTCCCTTCCT GATATGTCTC TGTCCACACA TCCATCTGTT 
CAGGCAGGTG GGTGGCGAAA GCTCCCGCCT AATCTCAGCC CCACTATCGA GTATAATGAC 
GGAAGGCCCA CAAAACGGCA TGATATTGCA CGCTCCCATT CTGAAAGTCC TTCCAGACTA 
CCAATCAACC GGGCGGGAAC CTGGAAGCGT GAACACAGCA AACATTCCTC GTCCCTTCCT 
CGAGTGAGTA CTTGGAGAAG AACTGGAAGC TCATCTTCTA TTCTTTCTGC TTCATCAGAG 
TCCAGTGAAA AAGCAAAAAG TGAGGATGAA AGGCATGTGA GCTCCATGCC AGCACCCAGA 
CAGATGAAGG AAAACCAGGT GCCCACCAAA GGAACATGGA GGAAAATCAA GGAAAGTGAC 
ATTTCTCCCA CAGGCATGGC TTCTCAGAGC GCTTCCTCAG GTGCTGCCAG TGGTGCTGAA 
TCCAAGCCTC TGATCTATCA GATGGCACCT CCTGTCTCTA AAACAGAGGA TGTTTGGGTG 
AGAATTGAGG ACTGCCCCAT TAACAACCCT AGATCTGGAC GGTCCCCCAC AGGCAACACC 
CCCCCAGTGA TTGACAGTGT TTCAGAGAAG GGAAGTTCAA GCATTAAAGA TTCAAAAGAC 
ACCCATGGGA AACAGAGTGT GGGCAGTGGC AGTCCTGTGC AAACCGTGGG TCTGGAAACC 
CGCCTCAACT CCTTTGTTCA GGTAGAGGCC CCAGAACAGA AAGGAACTGA GGCAAAACCA 
GGACAGAGTA ACCCAGTCTC TATAGCAGAG ACTGCTGAGA CGTGTATAGC AGAGCGTACC 
CCTTTCAGTT CCAGTAGCTC CAGCAAGCAC AGCTCACCTA GCGGGACTGT TGCTGCCAGA 
GTGACACCTT TTAATTACAA CCCTAGCCCT AGGAAGAGCA GCGCAGACAG CACTTCAGCC 
CGGCCGTCTC AGATCCCTAC GCCAGTGAGC ACCAACACGA AGAAGAGAGA TTCGAAGACT 
GACAGCACAG AATCCAGTGG AGCCCAAAGT CCTAAACGCC ATTCCGGGTC TTACCTCGTG 
ACGTCTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250277.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106137.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X4, mRNA.

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CloneID	OMu22391
Clone ID Related Accession (Same CDS sequence)	NM_007462.3 , XM_030250277.1
Accession Version	XM_030250277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGGGGACTG CAGGCCATTG CAGAGTTATT GCAGGTGGAC 
TGTGAGATGT ATGGGCTTAC TAATGACCAC TACAGTGTTA CTTTAAGACG GTATGCTGGA 
ATGGCTTTGA CAAACTTGAC CTTTGGAGAT GTTGCCAACA AGGCTACGCT GTGTTCTATG 
AAAGGCTGCA TGAGAGCACT TGTGGCCCAG TTAAAATCTG AGAGTGAAGA CTTACAGCAG 
GTTATTGCAA GTGTTTTGAG GAATTTGTCT TGGCGAGCAG ATGTAAATAG CAAAAAGACG 
TTGAGAGAAG TTGGAAGTGT GAAAGCATTG ATGGAATGTG CTTTGGAAGT TAAAAAGGAA 
TCAACCCTCA AAAGCGTTTT GAGTGCCTTA TGGAACCTGT CTGCACACTG CACTGAGAAT 
AAGGCTGACA TCTGTGCTGT GGATGGAGCA CTGGCATTTC TGGTTGGCAC CCTCACTTAC 
CGGAGCCAGA CAAATACTTT AGCCATTATT GAAAGTGGAG GTGGGATATT ACGGAATGTG 
TCCAGCTTGA TAGCTACAAA CGAAGACCAC AGGCAAATCC TAAGAGAGAA CAATTGCCTA 
CAAACTTTAT TACAGCACTT GAAATCTCAC AGCTTGACAA TAGTCAGTAA TGCATGTGGA 
ACTTTGTGGA ATCTCTCAGC AAGAAATCCT AAAGACCAGG AAGCCTTGTG GGACATGGGG 
GCAGTGAGCA TGCTCAAGAA CCTCATTCAT TCCAAGCACA AAATGATTGC CATGGGAAGT 
GCAGCAGCTT TAAGGAATCT CATGGCAAAC AGACCTGCAA AGTATAAGGA TGCCAATATC 
ATGTCTCCCG GCTCAAGTCT GCCATCCCTT CACGTTAGGA AACAGAAAGC TCTAGAAGCT 
GAGCTAGATG CTCAGCATTT ATCAGAAACC TTCGACAACA TTGACAACCT AAGTCCCAAG 
GCCTCTCACC GGAGTAAGCA GAGACACAAG CAGAATCTTT ATGGTGACTA TGCTTTTGAC 
GCCAATCGAC ATGATGATAG TAGGTCAGAC AATTTCAATA CTGGAAACAT GACTGTTCTT 
TCACCATATT TAAATACTAC GGTATTGCCC AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC 
AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG GAGAGAGAGC GAGGTATTGG CCTCAGTGCT 
TACCATCCAA CAACAGAAAA TGCAGGAACC TCATCAAAAC GAGGTCTGCA GATCACTACC 
ACTGCAGCCC AGATAGCCAA AGTTATGGAA GAAGTATCAG CCATTCATAC CTCCCAGGAC 
GACAGAAGTT CTGCTTCTAC CACCGAGTTC CATTGTGTGG CAGACGACAG GAGTGCGGCA 
CGAAGAAGCT CTGCCTCCCA CACACACTCA AACACATACA ACTTCACTAA GTCGGAAAAT 
TCAAATAGGA CATGCTCTAT GCCTTATGCC AAAGTGGAAT ATAAACGATC TTCAAATGAC 
AGTTTAAATA GTGTCACTAG TAGTGATGGA TATGGTAAAA GAGGCCAAAT GAAACCCTCA 
GTTGAATCCT ATTCTGAAGA TGATGAAAGT AAATTTTGCA GTTATGGTCA GTATCCAGCT 
GACCTAGCCC ATAAGATACA CAGTGCAAAT CATATGGATG ATAATGATGG AGAACTGGAT 
ACACCAATAA ATTACAGTCT TAAATATTCA GATGAGCAGT TGAACTCAGG AAGGCAGAGT 
CCCTCACAGA ATGAAAGGTG GGCAAGACCA AAGCATGTGA TAGAAGATGA AATAAAGCAA 
AACGAGCAAA GACAAGCAAG AAGCCAGAAC ACCAGTTATC CTGTCTATTC TGAGAATACC 
GATGACAAAC ACCTCAAATT CCAACCACAT TTTGGACAAC AAGAATGTGT TTCCCCATAT 
AGGTCAAGGG GAACCAGTGG TTCAGAAACA AATCGAATGG GTTCTAGTCA TGCAATTAAT 
CAAAATGTAA ACCAGTCTCT GTGTCAGGAA GATGATTATG AAGATGATAA ACCTACCAAC 
TACAGTGAAC GTTATTCTGA GGAAGAACAA CATGAAGAAG AAGAAGAGAG ACCGACAAAT 
TATAGCATAA AATATAATGA AGAGAAACAT CATGTGGATC AGCCTATTGA TTATAGTTTA 
AAATATGCCA CTGACATTTC TTCCTCACAA AAACCATCAT TTTCATTCTC AAAGAATTCA 
TCAGCACAAA GCACTAAACC TGAACATCTC TCTCCAAGCA GCGAGAATAC AGCTGTACCT 
CCATCTAATG CCAAAAGGCA GAATCAGCTG CGTCCAAGTT CAGCACAAAG AAATGGCCAG 
ACTCAAAAAG GCACTACTTG CAAAGTCCCC TCCATCAACC AAGAAACAAT ACAGACTTAC 
TGCGTAGAAG ACACCCCAAT ATGTTTTTCA AGGTGCAGTT CATTATCATC ACTGTCATCA 
GCTGACGATG AAATAGGATG TGATCAGACA ACACAGGAAG CAGATTCTGC TAATACTCTG 
CAGACAGCAG AAGTAAAAGA GAATGATGTA ACTCGGTCAG CTGAAGATCC TGCAACTGAA 
GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC AAACCCAGCC GACTCCAGGC TTCTGGCTTA 
TCTTCAGAAT CAACCAGGCA TAATAAAGCT GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC 
TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT CCCCCAGAAC ACTATGTCCA GGAGACTCCG 
CTCGTATTCA GCAGGTGTAC TTCTGTCAGC TCCCTTGACA GTTTTGAGAG TCGCTCCATT 
GCCAGCTCTG TTCAGAGTGA GCCATGTAGT GGAATGGTGA GTGGCATCAT AAGCCCCAGT 
GACCTTCCAG ATAGTCCTGG GCAGACCATG CCACCAAGCA GAAGCAAAAC CCCTCCACCT 
CCTCCACAGA CAGTGCAGGC CAAGAGAGAG GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG 
AAGAGAGAGA GTGGGCCTAA GCAGACTGCT GTAAATGCTG CCGTGCAGAG GGTGCAGGTC 
CTTCCAGACG TGGATACTTT GTTACACTTC GCCACAGAAA GTACTCCAGA CGGGTTTTCT 
TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG GATGAGCCAT TTATACAGAA AGATGTAGAA 
TTAAGAATCA TGCCTCCAGT TCAGGAAAAC GACAATGGGA ATGAAACTGA ATCAGAACAG 
CCTGAGGAAT CAAATGAAAA CCAGGATAAA GAGGTAGAAA AGCCTGACTC TGAAAAAGAC 
TTATTAGATG ATTCTGATGA CGATGATATT GAAATATTAG AAGAATGTAT TATTTCAGCC 
ATGCCAACAA AGTCATCACG CAAAGCCAAA AAACTAGCCC AGACTGCTTC AAAATTACCT 
CCACCTGTGG CAAGGAAACC AAGTCAGCTA CCTGTGTATA AACTTCTGCC AGCACAGAAT 
AGGCTGCAGG CACAAAAACA TGTTAGCTTT ACACCAGGGG ATGATGTGCC CCGGGTGTAC 
TGTGTAGAAG GGACACCTAT AAACTTTTCC ACAGCAACGT CTCTAAGTGA TCTGACAATA 
GAGTCCCCTC CAAATGAATT GGCTACTGGA GATGGGGTCA GAGCGGGTAT ACAGTCAGGT 
GAATTTGAAA AACGAGATAC CATTCCTACA GAAGGCAGAA GTACAGATGA TGCTCAGCGA 
GGAAAAATCT CATCTATAGT TACACCAGAC CTGGATGACA ACAAAGCAGA GGAAGGAGAT 
ATTCTTGCAG AATGTATCAA TTCTGCTATG CCCAAAGGAA AAAGCCACAA GCCTTTCCGA 
GTGAAAAAGA TAATGGACCA AGTCCAACAA GCATCCTCGA CTTCATCTGG AGCTAACAAA 
AATCAAGTAG ACACTAAGAA AAAGAAGCCT ACTTCACCAG TAAAGCCCAT GCCACAAAAT 
ACTGAATATA GAACGCGTGT GAGAAAGAAT ACAGACTCAA AAGTTAATGT AAATACTGAA 
GAAACTTTCT CAGACAACAA AGACTCAAAG AAACCAAGCT TACAAACCAA TGCCAAGGCC 
TTCAATGAAA AGCTACCTAA CAATGAAGAC AGAGTGCGGG GGAGCTTCGC CTTGGACTCA 
CCGCATCACT ACACCCCTAT TGAGGGGACG CCGTACTGCT TTTCCCGAAA TGACTCCTTG 
AGTTCTCTGG ATTTTGATGA TGACGATGTT GACCTTTCCA GGGAAAAGGC CGAGTTAAGA 
AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC AAAGTTACCT GCCGCCCAGA ACCAAACTCA 
AGCCAGCAGG CAGCTAGTAA GTCACAAGCC AGTATAAAAC ATCCAGCAAA CAGAGCACAG 
TCCAAACCAG TGCTGCAGAA ACAGCCCACT TTCCCCCAGT CCTCCAAAGA CGGACCAGAT 
AGAGGGGCAG CAACTGACGA AAAACTGCAG AATTTTGCTA TTGAAAATAC TCCAGTTTGC 
TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT AGTGACATTG ACCAGGAAAA CAACAATAAC 
AAAGAAAGTG AACCAATCAA AGAAGCTGAA CCTGCCAACT CACAAGGAGA GCCCAGTAAG 
CCTCAGGCAT CCGGGTATGC TCCCAAGTCC TTCCACGTCG AAGACACCCC TGTCTGTTTC 
TCAAGAAACA GCTCTCTCAG TTCTCTTAGC ATTGACTCTG AGGACGACCT GTTACAGGAG 
TGTATAAGTT CTGCCATGCC AAAAAAGAAA AGGCCTTCAA GACTCAAGAG TGAGAGCGAA 
AAGCAGAGCC CTAGAAAAGT GGGTGGCATA TTAGCTGAAG ACCTGACGCT TGATTTGAAA 
GATCTACAGA GGCCAGATTC AGAACACGCT TTCTCCCCCG ACTCAGAAAA TTTTGACTGG 
AAAGCTATTC AGGAAGGCGC AAACTCCATA GTAAGTAGTT TGCACCAAGC TGCTGCAGCC 
GCCGCGTGCT TATCTAGACA AGCGTCATCC GACTCAGATT CCATTCTGTC ACTAAAGTCC 
GGCATTTCTC TGGGATCGCC TTTTCATCTT ACACCTGATC AAGAGGAAAA GCCATTCACA 
AGCAATAAAG GCCCAAGAAT TCTCAAACCT GGAGAGAAAA GCACATTAGA AGCAAAAAAA 
ATAGAATCTG AAAACAAAGG AATCAAAGGC GGGAAAAAGG TTTATAAAAG CTTGATTACG 
GGAAAGATTC GCTCCAATTC AGAAATTTCC AGCCAAATGA AACAACCCCT CCCGACAAAC 
ATGCCTTCAA TCTCAAGAGG CAGGACGATG ATTCACATCC CAGGGCTTCG GAATAGCTCC 
TCTAGTACAA GCCCTGTCTC TAAGAAAGGC CCACCCCTCA AGACTCCAGC CTCTAAAAGC 
CCCAGTGAAG GGCCGGGAGC TACCACTTCT CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA 
GAGCTTAGCC CTATCACCAG GCAAACTTCC CAAATCAGTG GGTCAAATAA GGGGTCTTCT 
AGATCAGGAT CTAGAGACTC CACTCCCTCA AGACCTACAC AGCAACCATT AAGTAGGCCA 
ATGCAGTCTC CAGGGCGAAA CTCAATTTCC CCTGGTAGAA ATGGAATAAG CCCTCCTAAC 
AAACTGTCTC AGCTGCCCAG AACATCATCT CCCAGTACTG CTTCAACTAA GTCCTCCGGT 
TCTGGGAAAA TGTCATATAC ATCCCCAGGT AGACAGCTGA GCCAACAAAA TCTTACCAAA 
CAAGCAAGTT TATCCAAGAA TGCCAGCAGT ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA 
CTGAATCAGA TGAGTAACGG CAATGGGTCA AATAAAAAGG TAGAACTTTC TAGAATGTCT 
TCAACTAAAT CAAGTGGAAG TGAATCAGAC AGATCAGAAA GGCCTGCATT AGTACGCCAG 
TCTACTTTCA TCAAAGAAGC CCCAAGCCCA ACCCTGAGGA GGAAACTGGA GGAATCTGCC 
TCATTTGAAT CCCTTTCTCC ATCTTCTAGA CCAGATTCTC CCACCAGGTC GCAGGCACAG 
ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT ATGTCTCTGT CCACACATCC ATCTGTTCAG 
GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT CTCAGCCCCA CTATCGAGTA TAATGACGGA 
AGGCCCACAA AACGGCATGA TATTGCACGC TCCCATTCTG AAAGTCCTTC CAGACTACCA 
ATCAACCGGG CGGGAACCTG GAAGCGTGAA CACAGCAAAC ATTCCTCGTC CCTTCCTCGA 
GTGAGTACTT GGAGAAGAAC TGGAAGCTCA TCTTCTATTC TTTCTGCTTC ATCAGAGTCC 
AGTGAAAAAG CAAAAAGTGA GGATGAAAGG CATGTGAGCT CCATGCCAGC ACCCAGACAG 
ATGAAGGAAA ACCAGGTGCC CACCAAAGGA ACATGGAGGA AAATCAAGGA AAGTGACATT 
TCTCCCACAG GCATGGCTTC TCAGAGCGCT TCCTCAGGTG CTGCCAGTGG TGCTGAATCC 
AAGCCTCTGA TCTATCAGAT GGCACCTCCT GTCTCTAAAA CAGAGGATGT TTGGGTGAGA 
ATTGAGGACT GCCCCATTAA CAACCCTAGA TCTGGACGGT CCCCCACAGG CAACACCCCC 
CCAGTGATTG ACAGTGTTTC AGAGAAGGGA AGTTCAAGCA TTAAAGATTC AAAAGACACC 
CATGGGAAAC AGAGTGTGGG CAGTGGCAGT CCTGTGCAAA CCGTGGGTCT GGAAACCCGC 
CTCAACTCCT TTGTTCAGGT AGAGGCCCCA GAACAGAAAG GAACTGAGGC AAAACCAGGA 
CAGAGTAACC CAGTCTCTAT AGCAGAGACT GCTGAGACGT GTATAGCAGA GCGTACCCCT 
TTCAGTTCCA GTAGCTCCAG CAAGCACAGC TCACCTAGCG GGACTGTTGC TGCCAGAGTG 
ACACCTTTTA ATTACAACCC TAGCCCTAGG AAGAGCAGCG CAGACAGCAC TTCAGCCCGG 
CCGTCTCAGA TCCCTACGCC AGTGAGCACC AACACGAAGA AGAGAGATTC GAAGACTGAC 
AGCACAGAAT CCAGTGGAGC CCAAAGTCCT AAACGCCATT CCGGGTCTTA CCTCGTGACG 
TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106137.1
CDS	101..8629
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250277.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250277.1

ORF Insert Sequence:

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGGGGACTG CAGGCCATTG CAGAGTTATT GCAGGTGGAC 
TGTGAGATGT ATGGGCTTAC TAATGACCAC TACAGTGTTA CTTTAAGACG GTATGCTGGA 
ATGGCTTTGA CAAACTTGAC CTTTGGAGAT GTTGCCAACA AGGCTACGCT GTGTTCTATG 
AAAGGCTGCA TGAGAGCACT TGTGGCCCAG TTAAAATCTG AGAGTGAAGA CTTACAGCAG 
GTTATTGCAA GTGTTTTGAG GAATTTGTCT TGGCGAGCAG ATGTAAATAG CAAAAAGACG 
TTGAGAGAAG TTGGAAGTGT GAAAGCATTG ATGGAATGTG CTTTGGAAGT TAAAAAGGAA 
TCAACCCTCA AAAGCGTTTT GAGTGCCTTA TGGAACCTGT CTGCACACTG CACTGAGAAT 
AAGGCTGACA TCTGTGCTGT GGATGGAGCA CTGGCATTTC TGGTTGGCAC CCTCACTTAC 
CGGAGCCAGA CAAATACTTT AGCCATTATT GAAAGTGGAG GTGGGATATT ACGGAATGTG 
TCCAGCTTGA TAGCTACAAA CGAAGACCAC AGGCAAATCC TAAGAGAGAA CAATTGCCTA 
CAAACTTTAT TACAGCACTT GAAATCTCAC AGCTTGACAA TAGTCAGTAA TGCATGTGGA 
ACTTTGTGGA ATCTCTCAGC AAGAAATCCT AAAGACCAGG AAGCCTTGTG GGACATGGGG 
GCAGTGAGCA TGCTCAAGAA CCTCATTCAT TCCAAGCACA AAATGATTGC CATGGGAAGT 
GCAGCAGCTT TAAGGAATCT CATGGCAAAC AGACCTGCAA AGTATAAGGA TGCCAATATC 
ATGTCTCCCG GCTCAAGTCT GCCATCCCTT CACGTTAGGA AACAGAAAGC TCTAGAAGCT 
GAGCTAGATG CTCAGCATTT ATCAGAAACC TTCGACAACA TTGACAACCT AAGTCCCAAG 
GCCTCTCACC GGAGTAAGCA GAGACACAAG CAGAATCTTT ATGGTGACTA TGCTTTTGAC 
GCCAATCGAC ATGATGATAG TAGGTCAGAC AATTTCAATA CTGGAAACAT GACTGTTCTT 
TCACCATATT TAAATACTAC GGTATTGCCC AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC 
AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG GAGAGAGAGC GAGGTATTGG CCTCAGTGCT 
TACCATCCAA CAACAGAAAA TGCAGGAACC TCATCAAAAC GAGGTCTGCA GATCACTACC 
ACTGCAGCCC AGATAGCCAA AGTTATGGAA GAAGTATCAG CCATTCATAC CTCCCAGGAC 
GACAGAAGTT CTGCTTCTAC CACCGAGTTC CATTGTGTGG CAGACGACAG GAGTGCGGCA 
CGAAGAAGCT CTGCCTCCCA CACACACTCA AACACATACA ACTTCACTAA GTCGGAAAAT 
TCAAATAGGA CATGCTCTAT GCCTTATGCC AAAGTGGAAT ATAAACGATC TTCAAATGAC 
AGTTTAAATA GTGTCACTAG TAGTGATGGA TATGGTAAAA GAGGCCAAAT GAAACCCTCA 
GTTGAATCCT ATTCTGAAGA TGATGAAAGT AAATTTTGCA GTTATGGTCA GTATCCAGCT 
GACCTAGCCC ATAAGATACA CAGTGCAAAT CATATGGATG ATAATGATGG AGAACTGGAT 
ACACCAATAA ATTACAGTCT TAAATATTCA GATGAGCAGT TGAACTCAGG AAGGCAGAGT 
CCCTCACAGA ATGAAAGGTG GGCAAGACCA AAGCATGTGA TAGAAGATGA AATAAAGCAA 
AACGAGCAAA GACAAGCAAG AAGCCAGAAC ACCAGTTATC CTGTCTATTC TGAGAATACC 
GATGACAAAC ACCTCAAATT CCAACCACAT TTTGGACAAC AAGAATGTGT TTCCCCATAT 
AGGTCAAGGG GAACCAGTGG TTCAGAAACA AATCGAATGG GTTCTAGTCA TGCAATTAAT 
CAAAATGTAA ACCAGTCTCT GTGTCAGGAA GATGATTATG AAGATGATAA ACCTACCAAC 
TACAGTGAAC GTTATTCTGA GGAAGAACAA CATGAAGAAG AAGAAGAGAG ACCGACAAAT 
TATAGCATAA AATATAATGA AGAGAAACAT CATGTGGATC AGCCTATTGA TTATAGTTTA 
AAATATGCCA CTGACATTTC TTCCTCACAA AAACCATCAT TTTCATTCTC AAAGAATTCA 
TCAGCACAAA GCACTAAACC TGAACATCTC TCTCCAAGCA GCGAGAATAC AGCTGTACCT 
CCATCTAATG CCAAAAGGCA GAATCAGCTG CGTCCAAGTT CAGCACAAAG AAATGGCCAG 
ACTCAAAAAG GCACTACTTG CAAAGTCCCC TCCATCAACC AAGAAACAAT ACAGACTTAC 
TGCGTAGAAG ACACCCCAAT ATGTTTTTCA AGGTGCAGTT CATTATCATC ACTGTCATCA 
GCTGACGATG AAATAGGATG TGATCAGACA ACACAGGAAG CAGATTCTGC TAATACTCTG 
CAGACAGCAG AAGTAAAAGA GAATGATGTA ACTCGGTCAG CTGAAGATCC TGCAACTGAA 
GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC AAACCCAGCC GACTCCAGGC TTCTGGCTTA 
TCTTCAGAAT CAACCAGGCA TAATAAAGCT GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC 
TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT CCCCCAGAAC ACTATGTCCA GGAGACTCCG 
CTCGTATTCA GCAGGTGTAC TTCTGTCAGC TCCCTTGACA GTTTTGAGAG TCGCTCCATT 
GCCAGCTCTG TTCAGAGTGA GCCATGTAGT GGAATGGTGA GTGGCATCAT AAGCCCCAGT 
GACCTTCCAG ATAGTCCTGG GCAGACCATG CCACCAAGCA GAAGCAAAAC CCCTCCACCT 
CCTCCACAGA CAGTGCAGGC CAAGAGAGAG GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG 
AAGAGAGAGA GTGGGCCTAA GCAGACTGCT GTAAATGCTG CCGTGCAGAG GGTGCAGGTC 
CTTCCAGACG TGGATACTTT GTTACACTTC GCCACAGAAA GTACTCCAGA CGGGTTTTCT 
TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG GATGAGCCAT TTATACAGAA AGATGTAGAA 
TTAAGAATCA TGCCTCCAGT TCAGGAAAAC GACAATGGGA ATGAAACTGA ATCAGAACAG 
CCTGAGGAAT CAAATGAAAA CCAGGATAAA GAGGTAGAAA AGCCTGACTC TGAAAAAGAC 
TTATTAGATG ATTCTGATGA CGATGATATT GAAATATTAG AAGAATGTAT TATTTCAGCC 
ATGCCAACAA AGTCATCACG CAAAGCCAAA AAACTAGCCC AGACTGCTTC AAAATTACCT 
CCACCTGTGG CAAGGAAACC AAGTCAGCTA CCTGTGTATA AACTTCTGCC AGCACAGAAT 
AGGCTGCAGG CACAAAAACA TGTTAGCTTT ACACCAGGGG ATGATGTGCC CCGGGTGTAC 
TGTGTAGAAG GGACACCTAT AAACTTTTCC ACAGCAACGT CTCTAAGTGA TCTGACAATA 
GAGTCCCCTC CAAATGAATT GGCTACTGGA GATGGGGTCA GAGCGGGTAT ACAGTCAGGT 
GAATTTGAAA AACGAGATAC CATTCCTACA GAAGGCAGAA GTACAGATGA TGCTCAGCGA 
GGAAAAATCT CATCTATAGT TACACCAGAC CTGGATGACA ACAAAGCAGA GGAAGGAGAT 
ATTCTTGCAG AATGTATCAA TTCTGCTATG CCCAAAGGAA AAAGCCACAA GCCTTTCCGA 
GTGAAAAAGA TAATGGACCA AGTCCAACAA GCATCCTCGA CTTCATCTGG AGCTAACAAA 
AATCAAGTAG ACACTAAGAA AAAGAAGCCT ACTTCACCAG TAAAGCCCAT GCCACAAAAT 
ACTGAATATA GAACGCGTGT GAGAAAGAAT ACAGACTCAA AAGTTAATGT AAATACTGAA 
GAAACTTTCT CAGACAACAA AGACTCAAAG AAACCAAGCT TACAAACCAA TGCCAAGGCC 
TTCAATGAAA AGCTACCTAA CAATGAAGAC AGAGTGCGGG GGAGCTTCGC CTTGGACTCA 
CCGCATCACT ACACCCCTAT TGAGGGGACG CCGTACTGCT TTTCCCGAAA TGACTCCTTG 
AGTTCTCTGG ATTTTGATGA TGACGATGTT GACCTTTCCA GGGAAAAGGC CGAGTTAAGA 
AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC AAAGTTACCT GCCGCCCAGA ACCAAACTCA 
AGCCAGCAGG CAGCTAGTAA GTCACAAGCC AGTATAAAAC ATCCAGCAAA CAGAGCACAG 
TCCAAACCAG TGCTGCAGAA ACAGCCCACT TTCCCCCAGT CCTCCAAAGA CGGACCAGAT 
AGAGGGGCAG CAACTGACGA AAAACTGCAG AATTTTGCTA TTGAAAATAC TCCAGTTTGC 
TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT AGTGACATTG ACCAGGAAAA CAACAATAAC 
AAAGAAAGTG AACCAATCAA AGAAGCTGAA CCTGCCAACT CACAAGGAGA GCCCAGTAAG 
CCTCAGGCAT CCGGGTATGC TCCCAAGTCC TTCCACGTCG AAGACACCCC TGTCTGTTTC 
TCAAGAAACA GCTCTCTCAG TTCTCTTAGC ATTGACTCTG AGGACGACCT GTTACAGGAG 
TGTATAAGTT CTGCCATGCC AAAAAAGAAA AGGCCTTCAA GACTCAAGAG TGAGAGCGAA 
AAGCAGAGCC CTAGAAAAGT GGGTGGCATA TTAGCTGAAG ACCTGACGCT TGATTTGAAA 
GATCTACAGA GGCCAGATTC AGAACACGCT TTCTCCCCCG ACTCAGAAAA TTTTGACTGG 
AAAGCTATTC AGGAAGGCGC AAACTCCATA GTAAGTAGTT TGCACCAAGC TGCTGCAGCC 
GCCGCGTGCT TATCTAGACA AGCGTCATCC GACTCAGATT CCATTCTGTC ACTAAAGTCC 
GGCATTTCTC TGGGATCGCC TTTTCATCTT ACACCTGATC AAGAGGAAAA GCCATTCACA 
AGCAATAAAG GCCCAAGAAT TCTCAAACCT GGAGAGAAAA GCACATTAGA AGCAAAAAAA 
ATAGAATCTG AAAACAAAGG AATCAAAGGC GGGAAAAAGG TTTATAAAAG CTTGATTACG 
GGAAAGATTC GCTCCAATTC AGAAATTTCC AGCCAAATGA AACAACCCCT CCCGACAAAC 
ATGCCTTCAA TCTCAAGAGG CAGGACGATG ATTCACATCC CAGGGCTTCG GAATAGCTCC 
TCTAGTACAA GCCCTGTCTC TAAGAAAGGC CCACCCCTCA AGACTCCAGC CTCTAAAAGC 
CCCAGTGAAG GGCCGGGAGC TACCACTTCT CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA 
GAGCTTAGCC CTATCACCAG GCAAACTTCC CAAATCAGTG GGTCAAATAA GGGGTCTTCT 
AGATCAGGAT CTAGAGACTC CACTCCCTCA AGACCTACAC AGCAACCATT AAGTAGGCCA 
ATGCAGTCTC CAGGGCGAAA CTCAATTTCC CCTGGTAGAA ATGGAATAAG CCCTCCTAAC 
AAACTGTCTC AGCTGCCCAG AACATCATCT CCCAGTACTG CTTCAACTAA GTCCTCCGGT 
TCTGGGAAAA TGTCATATAC ATCCCCAGGT AGACAGCTGA GCCAACAAAA TCTTACCAAA 
CAAGCAAGTT TATCCAAGAA TGCCAGCAGT ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA 
CTGAATCAGA TGAGTAACGG CAATGGGTCA AATAAAAAGG TAGAACTTTC TAGAATGTCT 
TCAACTAAAT CAAGTGGAAG TGAATCAGAC AGATCAGAAA GGCCTGCATT AGTACGCCAG 
TCTACTTTCA TCAAAGAAGC CCCAAGCCCA ACCCTGAGGA GGAAACTGGA GGAATCTGCC 
TCATTTGAAT CCCTTTCTCC ATCTTCTAGA CCAGATTCTC CCACCAGGTC GCAGGCACAG 
ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT ATGTCTCTGT CCACACATCC ATCTGTTCAG 
GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT CTCAGCCCCA CTATCGAGTA TAATGACGGA 
AGGCCCACAA AACGGCATGA TATTGCACGC TCCCATTCTG AAAGTCCTTC CAGACTACCA 
ATCAACCGGG CGGGAACCTG GAAGCGTGAA CACAGCAAAC ATTCCTCGTC CCTTCCTCGA 
GTGAGTACTT GGAGAAGAAC TGGAAGCTCA TCTTCTATTC TTTCTGCTTC ATCAGAGTCC 
AGTGAAAAAG CAAAAAGTGA GGATGAAAGG CATGTGAGCT CCATGCCAGC ACCCAGACAG 
ATGAAGGAAA ACCAGGTGCC CACCAAAGGA ACATGGAGGA AAATCAAGGA AAGTGACATT 
TCTCCCACAG GCATGGCTTC TCAGAGCGCT TCCTCAGGTG CTGCCAGTGG TGCTGAATCC 
AAGCCTCTGA TCTATCAGAT GGCACCTCCT GTCTCTAAAA CAGAGGATGT TTGGGTGAGA 
ATTGAGGACT GCCCCATTAA CAACCCTAGA TCTGGACGGT CCCCCACAGG CAACACCCCC 
CCAGTGATTG ACAGTGTTTC AGAGAAGGGA AGTTCAAGCA TTAAAGATTC AAAAGACACC 
CATGGGAAAC AGAGTGTGGG CAGTGGCAGT CCTGTGCAAA CCGTGGGTCT GGAAACCCGC 
CTCAACTCCT TTGTTCAGGT AGAGGCCCCA GAACAGAAAG GAACTGAGGC AAAACCAGGA 
CAGAGTAACC CAGTCTCTAT AGCAGAGACT GCTGAGACGT GTATAGCAGA GCGTACCCCT 
TTCAGTTCCA GTAGCTCCAG CAAGCACAGC TCACCTAGCG GGACTGTTGC TGCCAGAGTG 
ACACCTTTTA ATTACAACCC TAGCCCTAGG AAGAGCAGCG CAGACAGCAC TTCAGCCCGG 
CCGTCTCAGA TCCCTACGCC AGTGAGCACC AACACGAAGA AGAGAGATTC GAAGACTGAC 
AGCACAGAAT CCAGTGGAGC CCAAAGTCCT AAACGCCATT CCGGGTCTTA CCTCGTGACG 
TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525529.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525592.1 Mus musculus adenomatosis polyposis coli (Apc), transcript variant X1, mRNA.

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CloneID	OMu54317
Clone ID Related Accession (Same CDS sequence)	XM_006525529.3 , XM_006525529.4
Accession Version	XM_006525529.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525529.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGGTG 
GAAATGGTGT ATTCCTTGTT GTCAATGCTT GGTACTCATG ATAAGGACGA TATGTCACGA 
ACTTTGCTAG CTATGTCCAG CTCCCAAGAC AGCTGTATAT CCATGCGGCA GTCTGGATGT 
CTTCCTCTCC TCATCCAGCT TTTACATGGC AATGACAAAG ACTCTGTATT GTTGGGAAAT 
TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG GCCAGTGCAG CACTCCACAA CATCATTCAC 
TCACAGCCTG ATGACAAGAG AGGCAGGCGT GAAATCCGAG TCCTTCATCT TTTGGAACAG 
ATACGAGCTT ACTGTGAAAC CTGTTGGGAG TGGCAGGAAG CCCACGAACA AGGCATGGAC 
CAGGACAAAA ACCCAATGCC AGCTCCTGTT GAGCATCAGA TCTGTCCTGC TGTGTGTGTT 
CTAATGAAGC TTTCATTTGA TGAAGAGCAT AGGCATGCAA TGAATGAACT TGGTAGGAAG 
GCTACCCGGG GCATTTCATC CCAGGAGCTA GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC 
ATTGCAGAGT TATTGCAGGT GGACTGTGAG ATGTATGGGC TTACTAATGA CCACTACAGT 
GTTACTTTAA GACGGTATGC TGGAATGGCT TTGACAAACT TGACCTTTGG AGATGTTGCC 
AACAAGGCTA CGCTGTGTTC TATGAAAGGC TGCATGAGAG CACTTGTGGC CCAGTTAAAA 
TCTGAGAGTG AAGACTTACA GCAGGTTATT GCAAGTGTTT TGAGGAATTT GTCTTGGCGA 
GCAGATGTAA ATAGCAAAAA GACGTTGAGA GAAGTTGGAA GTGTGAAAGC ATTGATGGAA 
TGTGCTTTGG AAGTTAAAAA GGAATCAACC CTCAAAAGCG TTTTGAGTGC CTTATGGAAC 
CTGTCTGCAC ACTGCACTGA GAATAAGGCT GACATCTGTG CTGTGGATGG AGCACTGGCA 
TTTCTGGTTG GCACCCTCAC TTACCGGAGC CAGACAAATA CTTTAGCCAT TATTGAAAGT 
GGAGGTGGGA TATTACGGAA TGTGTCCAGC TTGATAGCTA CAAACGAAGA CCACAGGCAA 
ATCCTAAGAG AGAACAATTG CCTACAAACT TTATTACAGC ACTTGAAATC TCACAGCTTG 
ACAATAGTCA GTAATGCATG TGGAACTTTG TGGAATCTCT CAGCAAGAAA TCCTAAAGAC 
CAGGAAGCCT TGTGGGACAT GGGGGCAGTG AGCATGCTCA AGAACCTCAT TCATTCCAAG 
CACAAAATGA TTGCCATGGG AAGTGCAGCA GCTTTAAGGA ATCTCATGGC AAACAGACCT 
GCAAAGTATA AGGATGCCAA TATCATGTCT CCCGGCTCAA GTCTGCCATC CCTTCACGTT 
AGGAAACAGA AAGCTCTAGA AGCTGAGCTA GATGCTCAGC ATTTATCAGA AACCTTCGAC 
AACATTGACA ACCTAAGTCC CAAGGCCTCT CACCGGAGTA AGCAGAGACA CAAGCAGAAT 
CTTTATGGTG ACTATGCTTT TGACGCCAAT CGACATGATG ATAGTAGGTC AGACAATTTC 
AATACTGGAA ACATGACTGT TCTTTCACCA TATTTAAATA CTACGGTATT GCCCAGCTCT 
TCTTCCTCAA GGGGAAGTTT AGACAGTTCT CGTTCTGAGA AAGACAGAAG TTTGGAGAGA 
GAGCGAGGTA TTGGCCTCAG TGCTTACCAT CCAACAACAG AAAATGCAGG AACCTCATCA 
AAACGAGGTC TGCAGATCAC TACCACTGCA GCCCAGATAG CCAAAGTTAT GGAAGAAGTA 
TCAGCCATTC ATACCTCCCA GGACGACAGA AGTTCTGCTT CTACCACCGA GTTCCATTGT 
GTGGCAGACG ACAGGAGTGC GGCACGAAGA AGCTCTGCCT CCCACACACA CTCAAACACA 
TACAACTTCA CTAAGTCGGA AAATTCAAAT AGGACATGCT CTATGCCTTA TGCCAAAGTG 
GAATATAAAC GATCTTCAAA TGACAGTTTA AATAGTGTCA CTAGTAGTGA TGGATATGGT 
AAAAGAGGCC AAATGAAACC CTCAGTTGAA TCCTATTCTG AAGATGATGA AAGTAAATTT 
TGCAGTTATG GTCAGTATCC AGCTGACCTA GCCCATAAGA TACACAGTGC AAATCATATG 
GATGATAATG ATGGAGAACT GGATACACCA ATAAATTACA GTCTTAAATA TTCAGATGAG 
CAGTTGAACT CAGGAAGGCA GAGTCCCTCA CAGAATGAAA GGTGGGCAAG ACCAAAGCAT 
GTGATAGAAG ATGAAATAAA GCAAAACGAG CAAAGACAAG CAAGAAGCCA GAACACCAGT 
TATCCTGTCT ATTCTGAGAA TACCGATGAC AAACACCTCA AATTCCAACC ACATTTTGGA 
CAACAAGAAT GTGTTTCCCC ATATAGGTCA AGGGGAACCA GTGGTTCAGA AACAAATCGA 
ATGGGTTCTA GTCATGCAAT TAATCAAAAT GTAAACCAGT CTCTGTGTCA GGAAGATGAT 
TATGAAGATG ATAAACCTAC CAACTACAGT GAACGTTATT CTGAGGAAGA ACAACATGAA 
GAAGAAGAAG AGAGACCGAC AAATTATAGC ATAAAATATA ATGAAGAGAA ACATCATGTG 
GATCAGCCTA TTGATTATAG TTTAAAATAT GCCACTGACA TTTCTTCCTC ACAAAAACCA 
TCATTTTCAT TCTCAAAGAA TTCATCAGCA CAAAGCACTA AACCTGAACA TCTCTCTCCA 
AGCAGCGAGA ATACAGCTGT ACCTCCATCT AATGCCAAAA GGCAGAATCA GCTGCGTCCA 
AGTTCAGCAC AAAGAAATGG CCAGACTCAA AAAGGCACTA CTTGCAAAGT CCCCTCCATC 
AACCAAGAAA CAATACAGAC TTACTGCGTA GAAGACACCC CAATATGTTT TTCAAGGTGC 
AGTTCATTAT CATCACTGTC ATCAGCTGAC GATGAAATAG GATGTGATCA GACAACACAG 
GAAGCAGATT CTGCTAATAC TCTGCAGACA GCAGAAGTAA AAGAGAATGA TGTAACTCGG 
TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA GCAGTGTCCC AGAATGCTAG AGCCAAACCC 
AGCCGACTCC AGGCTTCTGG CTTATCTTCA GAATCAACCA GGCATAATAA AGCTGTTGAG 
TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA AGTGGTGCTC AGACACCCAA AAGTCCCCCA 
GAACACTATG TCCAGGAGAC TCCGCTCGTA TTCAGCAGGT GTACTTCTGT CAGCTCCCTT 
GACAGTTTTG AGAGTCGCTC CATTGCCAGC TCTGTTCAGA GTGAGCCATG TAGTGGAATG 
GTGAGTGGCA TCATAAGCCC CAGTGACCTT CCAGATAGTC CTGGGCAGAC CATGCCACCA 
AGCAGAAGCA AAACCCCTCC ACCTCCTCCA CAGACAGTGC AGGCCAAGAG AGAGGTGCCA 
AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT 
GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA GACGTGGATA CTTTGTTACA CTTCGCCACA 
GAAAGTACTC CAGACGGGTT TTCTTGTTCC TCCAGCCTAA GTGCTCTGAG CCTGGATGAG 
CCATTTATAC AGAAAGATGT AGAATTAAGA ATCATGCCTC CAGTTCAGGA AAACGACAAT 
GGGAATGAAA CTGAATCAGA ACAGCCTGAG GAATCAAATG AAAACCAGGA TAAAGAGGTA 
GAAAAGCCTG ACTCTGAAAA AGACTTATTA GATGATTCTG ATGACGATGA TATTGAAATA 
TTAGAAGAAT GTATTATTTC AGCCATGCCA ACAAAGTCAT CACGCAAAGC CAAAAAACTA 
GCCCAGACTG CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTACCTGTG 
TATAAACTTC TGCCAGCACA GAATAGGCTG CAGGCACAAA AACATGTTAG CTTTACACCA 
GGGGATGATG TGCCCCGGGT GTACTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCA 
ACGTCTCTAA GTGATCTGAC AATAGAGTCC CCTCCAAATG AATTGGCTAC TGGAGATGGG 
GTCAGAGCGG GTATACAGTC AGGTGAATTT GAAAAACGAG ATACCATTCC TACAGAAGGC 
AGAAGTACAG ATGATGCTCA GCGAGGAAAA ATCTCATCTA TAGTTACACC AGACCTGGAT 
GACAACAAAG CAGAGGAAGG AGATATTCTT GCAGAATGTA TCAATTCTGC TATGCCCAAA 
GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA AAGATAATGG ACCAAGTCCA ACAAGCATCC 
TCGACTTCAT CTGGAGCTAA CAAAAATCAA GTAGACACTA AGAAAAAGAA GCCTACTTCA 
CCAGTAAAGC CCATGCCACA AAATACTGAA TATAGAACGC GTGTGAGAAA GAATACAGAC 
TCAAAAGTTA ATGTAAATAC TGAAGAAACT TTCTCAGACA ACAAAGACTC AAAGAAACCA 
AGCTTACAAA CCAATGCCAA GGCCTTCAAT GAAAAGCTAC CTAACAATGA AGACAGAGTG 
CGGGGGAGCT TCGCCTTGGA CTCACCGCAT CACTACACCC CTATTGAGGG GACGCCGTAC 
TGCTTTTCCC GAAATGACTC CTTGAGTTCT CTGGATTTTG ATGATGACGA TGTTGACCTT 
TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC AAAGAAAGCA AGGATTCCGA AGCCAAAGTT 
ACCTGCCGCC CAGAACCAAA CTCAAGCCAG CAGGCAGCTA GTAAGTCACA AGCCAGTATA 
AAACATCCAG CAAACAGAGC ACAGTCCAAA CCAGTGCTGC AGAAACAGCC CACTTTCCCC 
CAGTCCTCCA AAGACGGACC AGATAGAGGG GCAGCAACTG ACGAAAAACT GCAGAATTTT 
GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTGAGTTC CCTTAGTGAC 
ATTGACCAGG AAAACAACAA TAACAAAGAA AGTGAACCAA TCAAAGAAGC TGAACCTGCC 
AACTCACAAG GAGAGCCCAG TAAGCCTCAG GCATCCGGGT ATGCTCCCAA GTCCTTCCAC 
GTCGAAGACA CCCCTGTCTG TTTCTCAAGA AACAGCTCTC TCAGTTCTCT TAGCATTGAC 
TCTGAGGACG ACCTGTTACA GGAGTGTATA AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT 
TCAAGACTCA AGAGTGAGAG CGAAAAGCAG AGCCCTAGAA AAGTGGGTGG CATATTAGCT 
GAAGACCTGA CGCTTGATTT GAAAGATCTA CAGAGGCCAG ATTCAGAACA CGCTTTCTCC 
CCCGACTCAG AAAATTTTGA CTGGAAAGCT ATTCAGGAAG GCGCAAACTC CATAGTAAGT 
AGTTTGCACC AAGCTGCTGC AGCCGCCGCG TGCTTATCTA GACAAGCGTC ATCCGACTCA 
GATTCCATTC TGTCACTAAA GTCCGGCATT TCTCTGGGAT CGCCTTTTCA TCTTACACCT 
GATCAAGAGG AAAAGCCATT CACAAGCAAT AAAGGCCCAA GAATTCTCAA ACCTGGAGAG 
AAAAGCACAT TAGAAGCAAA AAAAATAGAA TCTGAAAACA AAGGAATCAA AGGCGGGAAA 
AAGGTTTATA AAAGCTTGAT TACGGGAAAG ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA 
ATGAAACAAC CCCTCCCGAC AAACATGCCT TCAATCTCAA GAGGCAGGAC GATGATTCAC 
ATCCCAGGGC TTCGGAATAG CTCCTCTAGT ACAAGCCCTG TCTCTAAGAA AGGCCCACCC 
CTCAAGACTC CAGCCTCTAA AAGCCCCAGT GAAGGGCCGG GAGCTACCAC TTCTCCTCGA 
GGAACTAAGC CAGCAGGAAA GTCAGAGCTT AGCCCTATCA CCAGGCAAAC TTCCCAAATC 
AGTGGGTCAA ATAAGGGGTC TTCTAGATCA GGATCTAGAG ACTCCACTCC CTCAAGACCT 
ACACAGCAAC CATTAAGTAG GCCAATGCAG TCTCCAGGGC GAAACTCAAT TTCCCCTGGT 
AGAAATGGAA TAAGCCCTCC TAACAAACTG TCTCAGCTGC CCAGAACATC ATCTCCCAGT 
ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG AAAATGTCAT ATACATCCCC AGGTAGACAG 
CTGAGCCAAC AAAATCTTAC CAAACAAGCA AGTTTATCCA AGAATGCCAG CAGTATCCCC 
AGAAGTGAGT CGGCATCTAA AGGACTGAAT CAGATGAGTA ACGGCAATGG GTCAAATAAA 
AAGGTAGAAC TTTCTAGAAT GTCTTCAACT AAATCAAGTG GAAGTGAATC AGACAGATCA 
GAAAGGCCTG CATTAGTACG CCAGTCTACT TTCATCAAAG AAGCCCCAAG CCCAACCCTG 
AGGAGGAAAC TGGAGGAATC TGCCTCATTT GAATCCCTTT CTCCATCTTC TAGACCAGAT 
TCTCCCACCA GGTCGCAGGC ACAGACCCCA GTTTTAAGCC CTTCCCTTCC TGATATGTCT 
CTGTCCACAC ATCCATCTGT TCAGGCAGGT GGGTGGCGAA AGCTCCCGCC TAATCTCAGC 
CCCACTATCG AGTATAATGA CGGAAGGCCC ACAAAACGGC ATGATATTGC ACGCTCCCAT 
TCTGAAAGTC CTTCCAGACT ACCAATCAAC CGGGCGGGAA CCTGGAAGCG TGAACACAGC 
AAACATTCCT CGTCCCTTCC TCGAGTGAGT ACTTGGAGAA GAACTGGAAG CTCATCTTCT 
ATTCTTTCTG CTTCATCAGA GTCCAGTGAA AAAGCAAAAA GTGAGGATGA AAGGCATGTG 
AGCTCCATGC CAGCACCCAG ACAGATGAAG GAAAACCAGG TGCCCACCAA AGGAACATGG 
AGGAAAATCA AGGAAAGTGA CATTTCTCCC ACAGGCATGG CTTCTCAGAG CGCTTCCTCA 
GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT CTGATCTATC AGATGGCACC TCCTGTCTCT 
AAAACAGAGG ATGTTTGGGT GAGAATTGAG GACTGCCCCA TTAACAACCC TAGATCTGGA 
CGGTCCCCCA CAGGCAACAC CCCCCCAGTG ATTGACAGTG TTTCAGAGAA GGGAAGTTCA 
AGCATTAAAG ATTCAAAAGA CACCCATGGG AAACAGAGTG TGGGCAGTGG CAGTCCTGTG 
CAAACCGTGG GTCTGGAAAC CCGCCTCAAC TCCTTTGTTC AGGTAGAGGC CCCAGAACAG 
AAAGGAACTG AGGCAAAACC AGGACAGAGT AACCCAGTCT CTATAGCAGA GACTGCTGAG 
ACGTGTATAG CAGAGCGTAC CCCTTTCAGT TCCAGTAGCT CCAGCAAGCA CAGCTCACCT 
AGCGGGACTG TTGCTGCCAG AGTGACACCT TTTAATTACA ACCCTAGCCC TAGGAAGAGC 
AGCGCAGACA GCACTTCAGC CCGGCCGTCT CAGATCCCTA CGCCAGTGAG CACCAACACG 
AAGAAGAGAG ATTCGAAGAC TGACAGCACA GAATCCAGTG GAGCCCAAAG TCCTAAACGC 
CATTCCGGGT CTTACCTCGT GACGTCTGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525592.1
CDS	496..9108
Translation	MYAFLRSGPVAALPASVPPVTLGARSSGGGRGRVPGEGRRPGGARACGRGASVWQEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525529.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525529.3

ORF Insert Sequence:

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGGTG 
GAAATGGTGT ATTCCTTGTT GTCAATGCTT GGTACTCATG ATAAGGACGA TATGTCACGA 
ACTTTGCTAG CTATGTCCAG CTCCCAAGAC AGCTGTATAT CCATGCGGCA GTCTGGATGT 
CTTCCTCTCC TCATCCAGCT TTTACATGGC AATGACAAAG ACTCTGTATT GTTGGGAAAT 
TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG GCCAGTGCAG CACTCCACAA CATCATTCAC 
TCACAGCCTG ATGACAAGAG AGGCAGGCGT GAAATCCGAG TCCTTCATCT TTTGGAACAG 
ATACGAGCTT ACTGTGAAAC CTGTTGGGAG TGGCAGGAAG CCCACGAACA AGGCATGGAC 
CAGGACAAAA ACCCAATGCC AGCTCCTGTT GAGCATCAGA TCTGTCCTGC TGTGTGTGTT 
CTAATGAAGC TTTCATTTGA TGAAGAGCAT AGGCATGCAA TGAATGAACT TGGTAGGAAG 
GCTACCCGGG GCATTTCATC CCAGGAGCTA GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC 
ATTGCAGAGT TATTGCAGGT GGACTGTGAG ATGTATGGGC TTACTAATGA CCACTACAGT 
GTTACTTTAA GACGGTATGC TGGAATGGCT TTGACAAACT TGACCTTTGG AGATGTTGCC 
AACAAGGCTA CGCTGTGTTC TATGAAAGGC TGCATGAGAG CACTTGTGGC CCAGTTAAAA 
TCTGAGAGTG AAGACTTACA GCAGGTTATT GCAAGTGTTT TGAGGAATTT GTCTTGGCGA 
GCAGATGTAA ATAGCAAAAA GACGTTGAGA GAAGTTGGAA GTGTGAAAGC ATTGATGGAA 
TGTGCTTTGG AAGTTAAAAA GGAATCAACC CTCAAAAGCG TTTTGAGTGC CTTATGGAAC 
CTGTCTGCAC ACTGCACTGA GAATAAGGCT GACATCTGTG CTGTGGATGG AGCACTGGCA 
TTTCTGGTTG GCACCCTCAC TTACCGGAGC CAGACAAATA CTTTAGCCAT TATTGAAAGT 
GGAGGTGGGA TATTACGGAA TGTGTCCAGC TTGATAGCTA CAAACGAAGA CCACAGGCAA 
ATCCTAAGAG AGAACAATTG CCTACAAACT TTATTACAGC ACTTGAAATC TCACAGCTTG 
ACAATAGTCA GTAATGCATG TGGAACTTTG TGGAATCTCT CAGCAAGAAA TCCTAAAGAC 
CAGGAAGCCT TGTGGGACAT GGGGGCAGTG AGCATGCTCA AGAACCTCAT TCATTCCAAG 
CACAAAATGA TTGCCATGGG AAGTGCAGCA GCTTTAAGGA ATCTCATGGC AAACAGACCT 
GCAAAGTATA AGGATGCCAA TATCATGTCT CCCGGCTCAA GTCTGCCATC CCTTCACGTT 
AGGAAACAGA AAGCTCTAGA AGCTGAGCTA GATGCTCAGC ATTTATCAGA AACCTTCGAC 
AACATTGACA ACCTAAGTCC CAAGGCCTCT CACCGGAGTA AGCAGAGACA CAAGCAGAAT 
CTTTATGGTG ACTATGCTTT TGACGCCAAT CGACATGATG ATAGTAGGTC AGACAATTTC 
AATACTGGAA ACATGACTGT TCTTTCACCA TATTTAAATA CTACGGTATT GCCCAGCTCT 
TCTTCCTCAA GGGGAAGTTT AGACAGTTCT CGTTCTGAGA AAGACAGAAG TTTGGAGAGA 
GAGCGAGGTA TTGGCCTCAG TGCTTACCAT CCAACAACAG AAAATGCAGG AACCTCATCA 
AAACGAGGTC TGCAGATCAC TACCACTGCA GCCCAGATAG CCAAAGTTAT GGAAGAAGTA 
TCAGCCATTC ATACCTCCCA GGACGACAGA AGTTCTGCTT CTACCACCGA GTTCCATTGT 
GTGGCAGACG ACAGGAGTGC GGCACGAAGA AGCTCTGCCT CCCACACACA CTCAAACACA 
TACAACTTCA CTAAGTCGGA AAATTCAAAT AGGACATGCT CTATGCCTTA TGCCAAAGTG 
GAATATAAAC GATCTTCAAA TGACAGTTTA AATAGTGTCA CTAGTAGTGA TGGATATGGT 
AAAAGAGGCC AAATGAAACC CTCAGTTGAA TCCTATTCTG AAGATGATGA AAGTAAATTT 
TGCAGTTATG GTCAGTATCC AGCTGACCTA GCCCATAAGA TACACAGTGC AAATCATATG 
GATGATAATG ATGGAGAACT GGATACACCA ATAAATTACA GTCTTAAATA TTCAGATGAG 
CAGTTGAACT CAGGAAGGCA GAGTCCCTCA CAGAATGAAA GGTGGGCAAG ACCAAAGCAT 
GTGATAGAAG ATGAAATAAA GCAAAACGAG CAAAGACAAG CAAGAAGCCA GAACACCAGT 
TATCCTGTCT ATTCTGAGAA TACCGATGAC AAACACCTCA AATTCCAACC ACATTTTGGA 
CAACAAGAAT GTGTTTCCCC ATATAGGTCA AGGGGAACCA GTGGTTCAGA AACAAATCGA 
ATGGGTTCTA GTCATGCAAT TAATCAAAAT GTAAACCAGT CTCTGTGTCA GGAAGATGAT 
TATGAAGATG ATAAACCTAC CAACTACAGT GAACGTTATT CTGAGGAAGA ACAACATGAA 
GAAGAAGAAG AGAGACCGAC AAATTATAGC ATAAAATATA ATGAAGAGAA ACATCATGTG 
GATCAGCCTA TTGATTATAG TTTAAAATAT GCCACTGACA TTTCTTCCTC ACAAAAACCA 
TCATTTTCAT TCTCAAAGAA TTCATCAGCA CAAAGCACTA AACCTGAACA TCTCTCTCCA 
AGCAGCGAGA ATACAGCTGT ACCTCCATCT AATGCCAAAA GGCAGAATCA GCTGCGTCCA 
AGTTCAGCAC AAAGAAATGG CCAGACTCAA AAAGGCACTA CTTGCAAAGT CCCCTCCATC 
AACCAAGAAA CAATACAGAC TTACTGCGTA GAAGACACCC CAATATGTTT TTCAAGGTGC 
AGTTCATTAT CATCACTGTC ATCAGCTGAC GATGAAATAG GATGTGATCA GACAACACAG 
GAAGCAGATT CTGCTAATAC TCTGCAGACA GCAGAAGTAA AAGAGAATGA TGTAACTCGG 
TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA GCAGTGTCCC AGAATGCTAG AGCCAAACCC 
AGCCGACTCC AGGCTTCTGG CTTATCTTCA GAATCAACCA GGCATAATAA AGCTGTTGAG 
TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA AGTGGTGCTC AGACACCCAA AAGTCCCCCA 
GAACACTATG TCCAGGAGAC TCCGCTCGTA TTCAGCAGGT GTACTTCTGT CAGCTCCCTT 
GACAGTTTTG AGAGTCGCTC CATTGCCAGC TCTGTTCAGA GTGAGCCATG TAGTGGAATG 
GTGAGTGGCA TCATAAGCCC CAGTGACCTT CCAGATAGTC CTGGGCAGAC CATGCCACCA 
AGCAGAAGCA AAACCCCTCC ACCTCCTCCA CAGACAGTGC AGGCCAAGAG AGAGGTGCCA 
AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT 
GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA GACGTGGATA CTTTGTTACA CTTCGCCACA 
GAAAGTACTC CAGACGGGTT TTCTTGTTCC TCCAGCCTAA GTGCTCTGAG CCTGGATGAG 
CCATTTATAC AGAAAGATGT AGAATTAAGA ATCATGCCTC CAGTTCAGGA AAACGACAAT 
GGGAATGAAA CTGAATCAGA ACAGCCTGAG GAATCAAATG AAAACCAGGA TAAAGAGGTA 
GAAAAGCCTG ACTCTGAAAA AGACTTATTA GATGATTCTG ATGACGATGA TATTGAAATA 
TTAGAAGAAT GTATTATTTC AGCCATGCCA ACAAAGTCAT CACGCAAAGC CAAAAAACTA 
GCCCAGACTG CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTACCTGTG 
TATAAACTTC TGCCAGCACA GAATAGGCTG CAGGCACAAA AACATGTTAG CTTTACACCA 
GGGGATGATG TGCCCCGGGT GTACTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCA 
ACGTCTCTAA GTGATCTGAC AATAGAGTCC CCTCCAAATG AATTGGCTAC TGGAGATGGG 
GTCAGAGCGG GTATACAGTC AGGTGAATTT GAAAAACGAG ATACCATTCC TACAGAAGGC 
AGAAGTACAG ATGATGCTCA GCGAGGAAAA ATCTCATCTA TAGTTACACC AGACCTGGAT 
GACAACAAAG CAGAGGAAGG AGATATTCTT GCAGAATGTA TCAATTCTGC TATGCCCAAA 
GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA AAGATAATGG ACCAAGTCCA ACAAGCATCC 
TCGACTTCAT CTGGAGCTAA CAAAAATCAA GTAGACACTA AGAAAAAGAA GCCTACTTCA 
CCAGTAAAGC CCATGCCACA AAATACTGAA TATAGAACGC GTGTGAGAAA GAATACAGAC 
TCAAAAGTTA ATGTAAATAC TGAAGAAACT TTCTCAGACA ACAAAGACTC AAAGAAACCA 
AGCTTACAAA CCAATGCCAA GGCCTTCAAT GAAAAGCTAC CTAACAATGA AGACAGAGTG 
CGGGGGAGCT TCGCCTTGGA CTCACCGCAT CACTACACCC CTATTGAGGG GACGCCGTAC 
TGCTTTTCCC GAAATGACTC CTTGAGTTCT CTGGATTTTG ATGATGACGA TGTTGACCTT 
TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC AAAGAAAGCA AGGATTCCGA AGCCAAAGTT 
ACCTGCCGCC CAGAACCAAA CTCAAGCCAG CAGGCAGCTA GTAAGTCACA AGCCAGTATA 
AAACATCCAG CAAACAGAGC ACAGTCCAAA CCAGTGCTGC AGAAACAGCC CACTTTCCCC 
CAGTCCTCCA AAGACGGACC AGATAGAGGG GCAGCAACTG ACGAAAAACT GCAGAATTTT 
GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTGAGTTC CCTTAGTGAC 
ATTGACCAGG AAAACAACAA TAACAAAGAA AGTGAACCAA TCAAAGAAGC TGAACCTGCC 
AACTCACAAG GAGAGCCCAG TAAGCCTCAG GCATCCGGGT ATGCTCCCAA GTCCTTCCAC 
GTCGAAGACA CCCCTGTCTG TTTCTCAAGA AACAGCTCTC TCAGTTCTCT TAGCATTGAC 
TCTGAGGACG ACCTGTTACA GGAGTGTATA AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT 
TCAAGACTCA AGAGTGAGAG CGAAAAGCAG AGCCCTAGAA AAGTGGGTGG CATATTAGCT 
GAAGACCTGA CGCTTGATTT GAAAGATCTA CAGAGGCCAG ATTCAGAACA CGCTTTCTCC 
CCCGACTCAG AAAATTTTGA CTGGAAAGCT ATTCAGGAAG GCGCAAACTC CATAGTAAGT 
AGTTTGCACC AAGCTGCTGC AGCCGCCGCG TGCTTATCTA GACAAGCGTC ATCCGACTCA 
GATTCCATTC TGTCACTAAA GTCCGGCATT TCTCTGGGAT CGCCTTTTCA TCTTACACCT 
GATCAAGAGG AAAAGCCATT CACAAGCAAT AAAGGCCCAA GAATTCTCAA ACCTGGAGAG 
AAAAGCACAT TAGAAGCAAA AAAAATAGAA TCTGAAAACA AAGGAATCAA AGGCGGGAAA 
AAGGTTTATA AAAGCTTGAT TACGGGAAAG ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA 
ATGAAACAAC CCCTCCCGAC AAACATGCCT TCAATCTCAA GAGGCAGGAC GATGATTCAC 
ATCCCAGGGC TTCGGAATAG CTCCTCTAGT ACAAGCCCTG TCTCTAAGAA AGGCCCACCC 
CTCAAGACTC CAGCCTCTAA AAGCCCCAGT GAAGGGCCGG GAGCTACCAC TTCTCCTCGA 
GGAACTAAGC CAGCAGGAAA GTCAGAGCTT AGCCCTATCA CCAGGCAAAC TTCCCAAATC 
AGTGGGTCAA ATAAGGGGTC TTCTAGATCA GGATCTAGAG ACTCCACTCC CTCAAGACCT 
ACACAGCAAC CATTAAGTAG GCCAATGCAG TCTCCAGGGC GAAACTCAAT TTCCCCTGGT 
AGAAATGGAA TAAGCCCTCC TAACAAACTG TCTCAGCTGC CCAGAACATC ATCTCCCAGT 
ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG AAAATGTCAT ATACATCCCC AGGTAGACAG 
CTGAGCCAAC AAAATCTTAC CAAACAAGCA AGTTTATCCA AGAATGCCAG CAGTATCCCC 
AGAAGTGAGT CGGCATCTAA AGGACTGAAT CAGATGAGTA ACGGCAATGG GTCAAATAAA 
AAGGTAGAAC TTTCTAGAAT GTCTTCAACT AAATCAAGTG GAAGTGAATC AGACAGATCA 
GAAAGGCCTG CATTAGTACG CCAGTCTACT TTCATCAAAG AAGCCCCAAG CCCAACCCTG 
AGGAGGAAAC TGGAGGAATC TGCCTCATTT GAATCCCTTT CTCCATCTTC TAGACCAGAT 
TCTCCCACCA GGTCGCAGGC ACAGACCCCA GTTTTAAGCC CTTCCCTTCC TGATATGTCT 
CTGTCCACAC ATCCATCTGT TCAGGCAGGT GGGTGGCGAA AGCTCCCGCC TAATCTCAGC 
CCCACTATCG AGTATAATGA CGGAAGGCCC ACAAAACGGC ATGATATTGC ACGCTCCCAT 
TCTGAAAGTC CTTCCAGACT ACCAATCAAC CGGGCGGGAA CCTGGAAGCG TGAACACAGC 
AAACATTCCT CGTCCCTTCC TCGAGTGAGT ACTTGGAGAA GAACTGGAAG CTCATCTTCT 
ATTCTTTCTG CTTCATCAGA GTCCAGTGAA AAAGCAAAAA GTGAGGATGA AAGGCATGTG 
AGCTCCATGC CAGCACCCAG ACAGATGAAG GAAAACCAGG TGCCCACCAA AGGAACATGG 
AGGAAAATCA AGGAAAGTGA CATTTCTCCC ACAGGCATGG CTTCTCAGAG CGCTTCCTCA 
GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT CTGATCTATC AGATGGCACC TCCTGTCTCT 
AAAACAGAGG ATGTTTGGGT GAGAATTGAG GACTGCCCCA TTAACAACCC TAGATCTGGA 
CGGTCCCCCA CAGGCAACAC CCCCCCAGTG ATTGACAGTG TTTCAGAGAA GGGAAGTTCA 
AGCATTAAAG ATTCAAAAGA CACCCATGGG AAACAGAGTG TGGGCAGTGG CAGTCCTGTG 
CAAACCGTGG GTCTGGAAAC CCGCCTCAAC TCCTTTGTTC AGGTAGAGGC CCCAGAACAG 
AAAGGAACTG AGGCAAAACC AGGACAGAGT AACCCAGTCT CTATAGCAGA GACTGCTGAG 
ACGTGTATAG CAGAGCGTAC CCCTTTCAGT TCCAGTAGCT CCAGCAAGCA CAGCTCACCT 
AGCGGGACTG TTGCTGCCAG AGTGACACCT TTTAATTACA ACCCTAGCCC TAGGAAGAGC 
AGCGCAGACA GCACTTCAGC CCGGCCGTCT CAGATCCCTA CGCCAGTGAG CACCAACACG 
AAGAAGAGAG ATTCGAAGAC TGACAGCACA GAATCCAGTG GAGCCCAAAG TCCTAAACGC 
CATTCCGGGT CTTACCTCGT GACGTCTGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525529.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525592.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X1, mRNA.

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CloneID	OMu54317
Clone ID Related Accession (Same CDS sequence)	XM_006525529.3 , XM_006525529.4
Accession Version	XM_006525529.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525529.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGGTG 
GAAATGGTGT ATTCCTTGTT GTCAATGCTT GGTACTCATG ATAAGGACGA TATGTCACGA 
ACTTTGCTAG CTATGTCCAG CTCCCAAGAC AGCTGTATAT CCATGCGGCA GTCTGGATGT 
CTTCCTCTCC TCATCCAGCT TTTACATGGC AATGACAAAG ACTCTGTATT GTTGGGAAAT 
TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG GCCAGTGCAG CACTCCACAA CATCATTCAC 
TCACAGCCTG ATGACAAGAG AGGCAGGCGT GAAATCCGAG TCCTTCATCT TTTGGAACAG 
ATACGAGCTT ACTGTGAAAC CTGTTGGGAG TGGCAGGAAG CCCACGAACA AGGCATGGAC 
CAGGACAAAA ACCCAATGCC AGCTCCTGTT GAGCATCAGA TCTGTCCTGC TGTGTGTGTT 
CTAATGAAGC TTTCATTTGA TGAAGAGCAT AGGCATGCAA TGAATGAACT TGGTAGGAAG 
GCTACCCGGG GCATTTCATC CCAGGAGCTA GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC 
ATTGCAGAGT TATTGCAGGT GGACTGTGAG ATGTATGGGC TTACTAATGA CCACTACAGT 
GTTACTTTAA GACGGTATGC TGGAATGGCT TTGACAAACT TGACCTTTGG AGATGTTGCC 
AACAAGGCTA CGCTGTGTTC TATGAAAGGC TGCATGAGAG CACTTGTGGC CCAGTTAAAA 
TCTGAGAGTG AAGACTTACA GCAGGTTATT GCAAGTGTTT TGAGGAATTT GTCTTGGCGA 
GCAGATGTAA ATAGCAAAAA GACGTTGAGA GAAGTTGGAA GTGTGAAAGC ATTGATGGAA 
TGTGCTTTGG AAGTTAAAAA GGAATCAACC CTCAAAAGCG TTTTGAGTGC CTTATGGAAC 
CTGTCTGCAC ACTGCACTGA GAATAAGGCT GACATCTGTG CTGTGGATGG AGCACTGGCA 
TTTCTGGTTG GCACCCTCAC TTACCGGAGC CAGACAAATA CTTTAGCCAT TATTGAAAGT 
GGAGGTGGGA TATTACGGAA TGTGTCCAGC TTGATAGCTA CAAACGAAGA CCACAGGCAA 
ATCCTAAGAG AGAACAATTG CCTACAAACT TTATTACAGC ACTTGAAATC TCACAGCTTG 
ACAATAGTCA GTAATGCATG TGGAACTTTG TGGAATCTCT CAGCAAGAAA TCCTAAAGAC 
CAGGAAGCCT TGTGGGACAT GGGGGCAGTG AGCATGCTCA AGAACCTCAT TCATTCCAAG 
CACAAAATGA TTGCCATGGG AAGTGCAGCA GCTTTAAGGA ATCTCATGGC AAACAGACCT 
GCAAAGTATA AGGATGCCAA TATCATGTCT CCCGGCTCAA GTCTGCCATC CCTTCACGTT 
AGGAAACAGA AAGCTCTAGA AGCTGAGCTA GATGCTCAGC ATTTATCAGA AACCTTCGAC 
AACATTGACA ACCTAAGTCC CAAGGCCTCT CACCGGAGTA AGCAGAGACA CAAGCAGAAT 
CTTTATGGTG ACTATGCTTT TGACGCCAAT CGACATGATG ATAGTAGGTC AGACAATTTC 
AATACTGGAA ACATGACTGT TCTTTCACCA TATTTAAATA CTACGGTATT GCCCAGCTCT 
TCTTCCTCAA GGGGAAGTTT AGACAGTTCT CGTTCTGAGA AAGACAGAAG TTTGGAGAGA 
GAGCGAGGTA TTGGCCTCAG TGCTTACCAT CCAACAACAG AAAATGCAGG AACCTCATCA 
AAACGAGGTC TGCAGATCAC TACCACTGCA GCCCAGATAG CCAAAGTTAT GGAAGAAGTA 
TCAGCCATTC ATACCTCCCA GGACGACAGA AGTTCTGCTT CTACCACCGA GTTCCATTGT 
GTGGCAGACG ACAGGAGTGC GGCACGAAGA AGCTCTGCCT CCCACACACA CTCAAACACA 
TACAACTTCA CTAAGTCGGA AAATTCAAAT AGGACATGCT CTATGCCTTA TGCCAAAGTG 
GAATATAAAC GATCTTCAAA TGACAGTTTA AATAGTGTCA CTAGTAGTGA TGGATATGGT 
AAAAGAGGCC AAATGAAACC CTCAGTTGAA TCCTATTCTG AAGATGATGA AAGTAAATTT 
TGCAGTTATG GTCAGTATCC AGCTGACCTA GCCCATAAGA TACACAGTGC AAATCATATG 
GATGATAATG ATGGAGAACT GGATACACCA ATAAATTACA GTCTTAAATA TTCAGATGAG 
CAGTTGAACT CAGGAAGGCA GAGTCCCTCA CAGAATGAAA GGTGGGCAAG ACCAAAGCAT 
GTGATAGAAG ATGAAATAAA GCAAAACGAG CAAAGACAAG CAAGAAGCCA GAACACCAGT 
TATCCTGTCT ATTCTGAGAA TACCGATGAC AAACACCTCA AATTCCAACC ACATTTTGGA 
CAACAAGAAT GTGTTTCCCC ATATAGGTCA AGGGGAACCA GTGGTTCAGA AACAAATCGA 
ATGGGTTCTA GTCATGCAAT TAATCAAAAT GTAAACCAGT CTCTGTGTCA GGAAGATGAT 
TATGAAGATG ATAAACCTAC CAACTACAGT GAACGTTATT CTGAGGAAGA ACAACATGAA 
GAAGAAGAAG AGAGACCGAC AAATTATAGC ATAAAATATA ATGAAGAGAA ACATCATGTG 
GATCAGCCTA TTGATTATAG TTTAAAATAT GCCACTGACA TTTCTTCCTC ACAAAAACCA 
TCATTTTCAT TCTCAAAGAA TTCATCAGCA CAAAGCACTA AACCTGAACA TCTCTCTCCA 
AGCAGCGAGA ATACAGCTGT ACCTCCATCT AATGCCAAAA GGCAGAATCA GCTGCGTCCA 
AGTTCAGCAC AAAGAAATGG CCAGACTCAA AAAGGCACTA CTTGCAAAGT CCCCTCCATC 
AACCAAGAAA CAATACAGAC TTACTGCGTA GAAGACACCC CAATATGTTT TTCAAGGTGC 
AGTTCATTAT CATCACTGTC ATCAGCTGAC GATGAAATAG GATGTGATCA GACAACACAG 
GAAGCAGATT CTGCTAATAC TCTGCAGACA GCAGAAGTAA AAGAGAATGA TGTAACTCGG 
TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA GCAGTGTCCC AGAATGCTAG AGCCAAACCC 
AGCCGACTCC AGGCTTCTGG CTTATCTTCA GAATCAACCA GGCATAATAA AGCTGTTGAG 
TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA AGTGGTGCTC AGACACCCAA AAGTCCCCCA 
GAACACTATG TCCAGGAGAC TCCGCTCGTA TTCAGCAGGT GTACTTCTGT CAGCTCCCTT 
GACAGTTTTG AGAGTCGCTC CATTGCCAGC TCTGTTCAGA GTGAGCCATG TAGTGGAATG 
GTGAGTGGCA TCATAAGCCC CAGTGACCTT CCAGATAGTC CTGGGCAGAC CATGCCACCA 
AGCAGAAGCA AAACCCCTCC ACCTCCTCCA CAGACAGTGC AGGCCAAGAG AGAGGTGCCA 
AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT 
GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA GACGTGGATA CTTTGTTACA CTTCGCCACA 
GAAAGTACTC CAGACGGGTT TTCTTGTTCC TCCAGCCTAA GTGCTCTGAG CCTGGATGAG 
CCATTTATAC AGAAAGATGT AGAATTAAGA ATCATGCCTC CAGTTCAGGA AAACGACAAT 
GGGAATGAAA CTGAATCAGA ACAGCCTGAG GAATCAAATG AAAACCAGGA TAAAGAGGTA 
GAAAAGCCTG ACTCTGAAAA AGACTTATTA GATGATTCTG ATGACGATGA TATTGAAATA 
TTAGAAGAAT GTATTATTTC AGCCATGCCA ACAAAGTCAT CACGCAAAGC CAAAAAACTA 
GCCCAGACTG CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTACCTGTG 
TATAAACTTC TGCCAGCACA GAATAGGCTG CAGGCACAAA AACATGTTAG CTTTACACCA 
GGGGATGATG TGCCCCGGGT GTACTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCA 
ACGTCTCTAA GTGATCTGAC AATAGAGTCC CCTCCAAATG AATTGGCTAC TGGAGATGGG 
GTCAGAGCGG GTATACAGTC AGGTGAATTT GAAAAACGAG ATACCATTCC TACAGAAGGC 
AGAAGTACAG ATGATGCTCA GCGAGGAAAA ATCTCATCTA TAGTTACACC AGACCTGGAT 
GACAACAAAG CAGAGGAAGG AGATATTCTT GCAGAATGTA TCAATTCTGC TATGCCCAAA 
GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA AAGATAATGG ACCAAGTCCA ACAAGCATCC 
TCGACTTCAT CTGGAGCTAA CAAAAATCAA GTAGACACTA AGAAAAAGAA GCCTACTTCA 
CCAGTAAAGC CCATGCCACA AAATACTGAA TATAGAACGC GTGTGAGAAA GAATACAGAC 
TCAAAAGTTA ATGTAAATAC TGAAGAAACT TTCTCAGACA ACAAAGACTC AAAGAAACCA 
AGCTTACAAA CCAATGCCAA GGCCTTCAAT GAAAAGCTAC CTAACAATGA AGACAGAGTG 
CGGGGGAGCT TCGCCTTGGA CTCACCGCAT CACTACACCC CTATTGAGGG GACGCCGTAC 
TGCTTTTCCC GAAATGACTC CTTGAGTTCT CTGGATTTTG ATGATGACGA TGTTGACCTT 
TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC AAAGAAAGCA AGGATTCCGA AGCCAAAGTT 
ACCTGCCGCC CAGAACCAAA CTCAAGCCAG CAGGCAGCTA GTAAGTCACA AGCCAGTATA 
AAACATCCAG CAAACAGAGC ACAGTCCAAA CCAGTGCTGC AGAAACAGCC CACTTTCCCC 
CAGTCCTCCA AAGACGGACC AGATAGAGGG GCAGCAACTG ACGAAAAACT GCAGAATTTT 
GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTGAGTTC CCTTAGTGAC 
ATTGACCAGG AAAACAACAA TAACAAAGAA AGTGAACCAA TCAAAGAAGC TGAACCTGCC 
AACTCACAAG GAGAGCCCAG TAAGCCTCAG GCATCCGGGT ATGCTCCCAA GTCCTTCCAC 
GTCGAAGACA CCCCTGTCTG TTTCTCAAGA AACAGCTCTC TCAGTTCTCT TAGCATTGAC 
TCTGAGGACG ACCTGTTACA GGAGTGTATA AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT 
TCAAGACTCA AGAGTGAGAG CGAAAAGCAG AGCCCTAGAA AAGTGGGTGG CATATTAGCT 
GAAGACCTGA CGCTTGATTT GAAAGATCTA CAGAGGCCAG ATTCAGAACA CGCTTTCTCC 
CCCGACTCAG AAAATTTTGA CTGGAAAGCT ATTCAGGAAG GCGCAAACTC CATAGTAAGT 
AGTTTGCACC AAGCTGCTGC AGCCGCCGCG TGCTTATCTA GACAAGCGTC ATCCGACTCA 
GATTCCATTC TGTCACTAAA GTCCGGCATT TCTCTGGGAT CGCCTTTTCA TCTTACACCT 
GATCAAGAGG AAAAGCCATT CACAAGCAAT AAAGGCCCAA GAATTCTCAA ACCTGGAGAG 
AAAAGCACAT TAGAAGCAAA AAAAATAGAA TCTGAAAACA AAGGAATCAA AGGCGGGAAA 
AAGGTTTATA AAAGCTTGAT TACGGGAAAG ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA 
ATGAAACAAC CCCTCCCGAC AAACATGCCT TCAATCTCAA GAGGCAGGAC GATGATTCAC 
ATCCCAGGGC TTCGGAATAG CTCCTCTAGT ACAAGCCCTG TCTCTAAGAA AGGCCCACCC 
CTCAAGACTC CAGCCTCTAA AAGCCCCAGT GAAGGGCCGG GAGCTACCAC TTCTCCTCGA 
GGAACTAAGC CAGCAGGAAA GTCAGAGCTT AGCCCTATCA CCAGGCAAAC TTCCCAAATC 
AGTGGGTCAA ATAAGGGGTC TTCTAGATCA GGATCTAGAG ACTCCACTCC CTCAAGACCT 
ACACAGCAAC CATTAAGTAG GCCAATGCAG TCTCCAGGGC GAAACTCAAT TTCCCCTGGT 
AGAAATGGAA TAAGCCCTCC TAACAAACTG TCTCAGCTGC CCAGAACATC ATCTCCCAGT 
ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG AAAATGTCAT ATACATCCCC AGGTAGACAG 
CTGAGCCAAC AAAATCTTAC CAAACAAGCA AGTTTATCCA AGAATGCCAG CAGTATCCCC 
AGAAGTGAGT CGGCATCTAA AGGACTGAAT CAGATGAGTA ACGGCAATGG GTCAAATAAA 
AAGGTAGAAC TTTCTAGAAT GTCTTCAACT AAATCAAGTG GAAGTGAATC AGACAGATCA 
GAAAGGCCTG CATTAGTACG CCAGTCTACT TTCATCAAAG AAGCCCCAAG CCCAACCCTG 
AGGAGGAAAC TGGAGGAATC TGCCTCATTT GAATCCCTTT CTCCATCTTC TAGACCAGAT 
TCTCCCACCA GGTCGCAGGC ACAGACCCCA GTTTTAAGCC CTTCCCTTCC TGATATGTCT 
CTGTCCACAC ATCCATCTGT TCAGGCAGGT GGGTGGCGAA AGCTCCCGCC TAATCTCAGC 
CCCACTATCG AGTATAATGA CGGAAGGCCC ACAAAACGGC ATGATATTGC ACGCTCCCAT 
TCTGAAAGTC CTTCCAGACT ACCAATCAAC CGGGCGGGAA CCTGGAAGCG TGAACACAGC 
AAACATTCCT CGTCCCTTCC TCGAGTGAGT ACTTGGAGAA GAACTGGAAG CTCATCTTCT 
ATTCTTTCTG CTTCATCAGA GTCCAGTGAA AAAGCAAAAA GTGAGGATGA AAGGCATGTG 
AGCTCCATGC CAGCACCCAG ACAGATGAAG GAAAACCAGG TGCCCACCAA AGGAACATGG 
AGGAAAATCA AGGAAAGTGA CATTTCTCCC ACAGGCATGG CTTCTCAGAG CGCTTCCTCA 
GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT CTGATCTATC AGATGGCACC TCCTGTCTCT 
AAAACAGAGG ATGTTTGGGT GAGAATTGAG GACTGCCCCA TTAACAACCC TAGATCTGGA 
CGGTCCCCCA CAGGCAACAC CCCCCCAGTG ATTGACAGTG TTTCAGAGAA GGGAAGTTCA 
AGCATTAAAG ATTCAAAAGA CACCCATGGG AAACAGAGTG TGGGCAGTGG CAGTCCTGTG 
CAAACCGTGG GTCTGGAAAC CCGCCTCAAC TCCTTTGTTC AGGTAGAGGC CCCAGAACAG 
AAAGGAACTG AGGCAAAACC AGGACAGAGT AACCCAGTCT CTATAGCAGA GACTGCTGAG 
ACGTGTATAG CAGAGCGTAC CCCTTTCAGT TCCAGTAGCT CCAGCAAGCA CAGCTCACCT 
AGCGGGACTG TTGCTGCCAG AGTGACACCT TTTAATTACA ACCCTAGCCC TAGGAAGAGC 
AGCGCAGACA GCACTTCAGC CCGGCCGTCT CAGATCCCTA CGCCAGTGAG CACCAACACG 
AAGAAGAGAG ATTCGAAGAC TGACAGCACA GAATCCAGTG GAGCCCAAAG TCCTAAACGC 
CATTCCGGGT CTTACCTCGT GACGTCTGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525592.1
CDS	816..9428
Translation	MYAFLRSGPVAALPASVPPVTLGARSSGGGRGRVPGEGRRPGGARACGRGASVWQEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525529.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525529.4

ORF Insert Sequence:

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGGTG 
GAAATGGTGT ATTCCTTGTT GTCAATGCTT GGTACTCATG ATAAGGACGA TATGTCACGA 
ACTTTGCTAG CTATGTCCAG CTCCCAAGAC AGCTGTATAT CCATGCGGCA GTCTGGATGT 
CTTCCTCTCC TCATCCAGCT TTTACATGGC AATGACAAAG ACTCTGTATT GTTGGGAAAT 
TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG GCCAGTGCAG CACTCCACAA CATCATTCAC 
TCACAGCCTG ATGACAAGAG AGGCAGGCGT GAAATCCGAG TCCTTCATCT TTTGGAACAG 
ATACGAGCTT ACTGTGAAAC CTGTTGGGAG TGGCAGGAAG CCCACGAACA AGGCATGGAC 
CAGGACAAAA ACCCAATGCC AGCTCCTGTT GAGCATCAGA TCTGTCCTGC TGTGTGTGTT 
CTAATGAAGC TTTCATTTGA TGAAGAGCAT AGGCATGCAA TGAATGAACT TGGTAGGAAG 
GCTACCCGGG GCATTTCATC CCAGGAGCTA GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC 
ATTGCAGAGT TATTGCAGGT GGACTGTGAG ATGTATGGGC TTACTAATGA CCACTACAGT 
GTTACTTTAA GACGGTATGC TGGAATGGCT TTGACAAACT TGACCTTTGG AGATGTTGCC 
AACAAGGCTA CGCTGTGTTC TATGAAAGGC TGCATGAGAG CACTTGTGGC CCAGTTAAAA 
TCTGAGAGTG AAGACTTACA GCAGGTTATT GCAAGTGTTT TGAGGAATTT GTCTTGGCGA 
GCAGATGTAA ATAGCAAAAA GACGTTGAGA GAAGTTGGAA GTGTGAAAGC ATTGATGGAA 
TGTGCTTTGG AAGTTAAAAA GGAATCAACC CTCAAAAGCG TTTTGAGTGC CTTATGGAAC 
CTGTCTGCAC ACTGCACTGA GAATAAGGCT GACATCTGTG CTGTGGATGG AGCACTGGCA 
TTTCTGGTTG GCACCCTCAC TTACCGGAGC CAGACAAATA CTTTAGCCAT TATTGAAAGT 
GGAGGTGGGA TATTACGGAA TGTGTCCAGC TTGATAGCTA CAAACGAAGA CCACAGGCAA 
ATCCTAAGAG AGAACAATTG CCTACAAACT TTATTACAGC ACTTGAAATC TCACAGCTTG 
ACAATAGTCA GTAATGCATG TGGAACTTTG TGGAATCTCT CAGCAAGAAA TCCTAAAGAC 
CAGGAAGCCT TGTGGGACAT GGGGGCAGTG AGCATGCTCA AGAACCTCAT TCATTCCAAG 
CACAAAATGA TTGCCATGGG AAGTGCAGCA GCTTTAAGGA ATCTCATGGC AAACAGACCT 
GCAAAGTATA AGGATGCCAA TATCATGTCT CCCGGCTCAA GTCTGCCATC CCTTCACGTT 
AGGAAACAGA AAGCTCTAGA AGCTGAGCTA GATGCTCAGC ATTTATCAGA AACCTTCGAC 
AACATTGACA ACCTAAGTCC CAAGGCCTCT CACCGGAGTA AGCAGAGACA CAAGCAGAAT 
CTTTATGGTG ACTATGCTTT TGACGCCAAT CGACATGATG ATAGTAGGTC AGACAATTTC 
AATACTGGAA ACATGACTGT TCTTTCACCA TATTTAAATA CTACGGTATT GCCCAGCTCT 
TCTTCCTCAA GGGGAAGTTT AGACAGTTCT CGTTCTGAGA AAGACAGAAG TTTGGAGAGA 
GAGCGAGGTA TTGGCCTCAG TGCTTACCAT CCAACAACAG AAAATGCAGG AACCTCATCA 
AAACGAGGTC TGCAGATCAC TACCACTGCA GCCCAGATAG CCAAAGTTAT GGAAGAAGTA 
TCAGCCATTC ATACCTCCCA GGACGACAGA AGTTCTGCTT CTACCACCGA GTTCCATTGT 
GTGGCAGACG ACAGGAGTGC GGCACGAAGA AGCTCTGCCT CCCACACACA CTCAAACACA 
TACAACTTCA CTAAGTCGGA AAATTCAAAT AGGACATGCT CTATGCCTTA TGCCAAAGTG 
GAATATAAAC GATCTTCAAA TGACAGTTTA AATAGTGTCA CTAGTAGTGA TGGATATGGT 
AAAAGAGGCC AAATGAAACC CTCAGTTGAA TCCTATTCTG AAGATGATGA AAGTAAATTT 
TGCAGTTATG GTCAGTATCC AGCTGACCTA GCCCATAAGA TACACAGTGC AAATCATATG 
GATGATAATG ATGGAGAACT GGATACACCA ATAAATTACA GTCTTAAATA TTCAGATGAG 
CAGTTGAACT CAGGAAGGCA GAGTCCCTCA CAGAATGAAA GGTGGGCAAG ACCAAAGCAT 
GTGATAGAAG ATGAAATAAA GCAAAACGAG CAAAGACAAG CAAGAAGCCA GAACACCAGT 
TATCCTGTCT ATTCTGAGAA TACCGATGAC AAACACCTCA AATTCCAACC ACATTTTGGA 
CAACAAGAAT GTGTTTCCCC ATATAGGTCA AGGGGAACCA GTGGTTCAGA AACAAATCGA 
ATGGGTTCTA GTCATGCAAT TAATCAAAAT GTAAACCAGT CTCTGTGTCA GGAAGATGAT 
TATGAAGATG ATAAACCTAC CAACTACAGT GAACGTTATT CTGAGGAAGA ACAACATGAA 
GAAGAAGAAG AGAGACCGAC AAATTATAGC ATAAAATATA ATGAAGAGAA ACATCATGTG 
GATCAGCCTA TTGATTATAG TTTAAAATAT GCCACTGACA TTTCTTCCTC ACAAAAACCA 
TCATTTTCAT TCTCAAAGAA TTCATCAGCA CAAAGCACTA AACCTGAACA TCTCTCTCCA 
AGCAGCGAGA ATACAGCTGT ACCTCCATCT AATGCCAAAA GGCAGAATCA GCTGCGTCCA 
AGTTCAGCAC AAAGAAATGG CCAGACTCAA AAAGGCACTA CTTGCAAAGT CCCCTCCATC 
AACCAAGAAA CAATACAGAC TTACTGCGTA GAAGACACCC CAATATGTTT TTCAAGGTGC 
AGTTCATTAT CATCACTGTC ATCAGCTGAC GATGAAATAG GATGTGATCA GACAACACAG 
GAAGCAGATT CTGCTAATAC TCTGCAGACA GCAGAAGTAA AAGAGAATGA TGTAACTCGG 
TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA GCAGTGTCCC AGAATGCTAG AGCCAAACCC 
AGCCGACTCC AGGCTTCTGG CTTATCTTCA GAATCAACCA GGCATAATAA AGCTGTTGAG 
TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA AGTGGTGCTC AGACACCCAA AAGTCCCCCA 
GAACACTATG TCCAGGAGAC TCCGCTCGTA TTCAGCAGGT GTACTTCTGT CAGCTCCCTT 
GACAGTTTTG AGAGTCGCTC CATTGCCAGC TCTGTTCAGA GTGAGCCATG TAGTGGAATG 
GTGAGTGGCA TCATAAGCCC CAGTGACCTT CCAGATAGTC CTGGGCAGAC CATGCCACCA 
AGCAGAAGCA AAACCCCTCC ACCTCCTCCA CAGACAGTGC AGGCCAAGAG AGAGGTGCCA 
AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT 
GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA GACGTGGATA CTTTGTTACA CTTCGCCACA 
GAAAGTACTC CAGACGGGTT TTCTTGTTCC TCCAGCCTAA GTGCTCTGAG CCTGGATGAG 
CCATTTATAC AGAAAGATGT AGAATTAAGA ATCATGCCTC CAGTTCAGGA AAACGACAAT 
GGGAATGAAA CTGAATCAGA ACAGCCTGAG GAATCAAATG AAAACCAGGA TAAAGAGGTA 
GAAAAGCCTG ACTCTGAAAA AGACTTATTA GATGATTCTG ATGACGATGA TATTGAAATA 
TTAGAAGAAT GTATTATTTC AGCCATGCCA ACAAAGTCAT CACGCAAAGC CAAAAAACTA 
GCCCAGACTG CTTCAAAATT ACCTCCACCT GTGGCAAGGA AACCAAGTCA GCTACCTGTG 
TATAAACTTC TGCCAGCACA GAATAGGCTG CAGGCACAAA AACATGTTAG CTTTACACCA 
GGGGATGATG TGCCCCGGGT GTACTGTGTA GAAGGGACAC CTATAAACTT TTCCACAGCA 
ACGTCTCTAA GTGATCTGAC AATAGAGTCC CCTCCAAATG AATTGGCTAC TGGAGATGGG 
GTCAGAGCGG GTATACAGTC AGGTGAATTT GAAAAACGAG ATACCATTCC TACAGAAGGC 
AGAAGTACAG ATGATGCTCA GCGAGGAAAA ATCTCATCTA TAGTTACACC AGACCTGGAT 
GACAACAAAG CAGAGGAAGG AGATATTCTT GCAGAATGTA TCAATTCTGC TATGCCCAAA 
GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA AAGATAATGG ACCAAGTCCA ACAAGCATCC 
TCGACTTCAT CTGGAGCTAA CAAAAATCAA GTAGACACTA AGAAAAAGAA GCCTACTTCA 
CCAGTAAAGC CCATGCCACA AAATACTGAA TATAGAACGC GTGTGAGAAA GAATACAGAC 
TCAAAAGTTA ATGTAAATAC TGAAGAAACT TTCTCAGACA ACAAAGACTC AAAGAAACCA 
AGCTTACAAA CCAATGCCAA GGCCTTCAAT GAAAAGCTAC CTAACAATGA AGACAGAGTG 
CGGGGGAGCT TCGCCTTGGA CTCACCGCAT CACTACACCC CTATTGAGGG GACGCCGTAC 
TGCTTTTCCC GAAATGACTC CTTGAGTTCT CTGGATTTTG ATGATGACGA TGTTGACCTT 
TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC AAAGAAAGCA AGGATTCCGA AGCCAAAGTT 
ACCTGCCGCC CAGAACCAAA CTCAAGCCAG CAGGCAGCTA GTAAGTCACA AGCCAGTATA 
AAACATCCAG CAAACAGAGC ACAGTCCAAA CCAGTGCTGC AGAAACAGCC CACTTTCCCC 
CAGTCCTCCA AAGACGGACC AGATAGAGGG GCAGCAACTG ACGAAAAACT GCAGAATTTT 
GCTATTGAAA ATACTCCAGT TTGCTTTTCT CGAAATTCCT CTCTGAGTTC CCTTAGTGAC 
ATTGACCAGG AAAACAACAA TAACAAAGAA AGTGAACCAA TCAAAGAAGC TGAACCTGCC 
AACTCACAAG GAGAGCCCAG TAAGCCTCAG GCATCCGGGT ATGCTCCCAA GTCCTTCCAC 
GTCGAAGACA CCCCTGTCTG TTTCTCAAGA AACAGCTCTC TCAGTTCTCT TAGCATTGAC 
TCTGAGGACG ACCTGTTACA GGAGTGTATA AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT 
TCAAGACTCA AGAGTGAGAG CGAAAAGCAG AGCCCTAGAA AAGTGGGTGG CATATTAGCT 
GAAGACCTGA CGCTTGATTT GAAAGATCTA CAGAGGCCAG ATTCAGAACA CGCTTTCTCC 
CCCGACTCAG AAAATTTTGA CTGGAAAGCT ATTCAGGAAG GCGCAAACTC CATAGTAAGT 
AGTTTGCACC AAGCTGCTGC AGCCGCCGCG TGCTTATCTA GACAAGCGTC ATCCGACTCA 
GATTCCATTC TGTCACTAAA GTCCGGCATT TCTCTGGGAT CGCCTTTTCA TCTTACACCT 
GATCAAGAGG AAAAGCCATT CACAAGCAAT AAAGGCCCAA GAATTCTCAA ACCTGGAGAG 
AAAAGCACAT TAGAAGCAAA AAAAATAGAA TCTGAAAACA AAGGAATCAA AGGCGGGAAA 
AAGGTTTATA AAAGCTTGAT TACGGGAAAG ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA 
ATGAAACAAC CCCTCCCGAC AAACATGCCT TCAATCTCAA GAGGCAGGAC GATGATTCAC 
ATCCCAGGGC TTCGGAATAG CTCCTCTAGT ACAAGCCCTG TCTCTAAGAA AGGCCCACCC 
CTCAAGACTC CAGCCTCTAA AAGCCCCAGT GAAGGGCCGG GAGCTACCAC TTCTCCTCGA 
GGAACTAAGC CAGCAGGAAA GTCAGAGCTT AGCCCTATCA CCAGGCAAAC TTCCCAAATC 
AGTGGGTCAA ATAAGGGGTC TTCTAGATCA GGATCTAGAG ACTCCACTCC CTCAAGACCT 
ACACAGCAAC CATTAAGTAG GCCAATGCAG TCTCCAGGGC GAAACTCAAT TTCCCCTGGT 
AGAAATGGAA TAAGCCCTCC TAACAAACTG TCTCAGCTGC CCAGAACATC ATCTCCCAGT 
ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG AAAATGTCAT ATACATCCCC AGGTAGACAG 
CTGAGCCAAC AAAATCTTAC CAAACAAGCA AGTTTATCCA AGAATGCCAG CAGTATCCCC 
AGAAGTGAGT CGGCATCTAA AGGACTGAAT CAGATGAGTA ACGGCAATGG GTCAAATAAA 
AAGGTAGAAC TTTCTAGAAT GTCTTCAACT AAATCAAGTG GAAGTGAATC AGACAGATCA 
GAAAGGCCTG CATTAGTACG CCAGTCTACT TTCATCAAAG AAGCCCCAAG CCCAACCCTG 
AGGAGGAAAC TGGAGGAATC TGCCTCATTT GAATCCCTTT CTCCATCTTC TAGACCAGAT 
TCTCCCACCA GGTCGCAGGC ACAGACCCCA GTTTTAAGCC CTTCCCTTCC TGATATGTCT 
CTGTCCACAC ATCCATCTGT TCAGGCAGGT GGGTGGCGAA AGCTCCCGCC TAATCTCAGC 
CCCACTATCG AGTATAATGA CGGAAGGCCC ACAAAACGGC ATGATATTGC ACGCTCCCAT 
TCTGAAAGTC CTTCCAGACT ACCAATCAAC CGGGCGGGAA CCTGGAAGCG TGAACACAGC 
AAACATTCCT CGTCCCTTCC TCGAGTGAGT ACTTGGAGAA GAACTGGAAG CTCATCTTCT 
ATTCTTTCTG CTTCATCAGA GTCCAGTGAA AAAGCAAAAA GTGAGGATGA AAGGCATGTG 
AGCTCCATGC CAGCACCCAG ACAGATGAAG GAAAACCAGG TGCCCACCAA AGGAACATGG 
AGGAAAATCA AGGAAAGTGA CATTTCTCCC ACAGGCATGG CTTCTCAGAG CGCTTCCTCA 
GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT CTGATCTATC AGATGGCACC TCCTGTCTCT 
AAAACAGAGG ATGTTTGGGT GAGAATTGAG GACTGCCCCA TTAACAACCC TAGATCTGGA 
CGGTCCCCCA CAGGCAACAC CCCCCCAGTG ATTGACAGTG TTTCAGAGAA GGGAAGTTCA 
AGCATTAAAG ATTCAAAAGA CACCCATGGG AAACAGAGTG TGGGCAGTGG CAGTCCTGTG 
CAAACCGTGG GTCTGGAAAC CCGCCTCAAC TCCTTTGTTC AGGTAGAGGC CCCAGAACAG 
AAAGGAACTG AGGCAAAACC AGGACAGAGT AACCCAGTCT CTATAGCAGA GACTGCTGAG 
ACGTGTATAG CAGAGCGTAC CCCTTTCAGT TCCAGTAGCT CCAGCAAGCA CAGCTCACCT 
AGCGGGACTG TTGCTGCCAG AGTGACACCT TTTAATTACA ACCCTAGCCC TAGGAAGAGC 
AGCGCAGACA GCACTTCAGC CCGGCCGTCT CAGATCCCTA CGCCAGTGAG CACCAACACG 
AAGAAGAGAG ATTCGAAGAC TGACAGCACA GAATCCAGTG GAGCCCAAAG TCCTAAACGC 
CATTCCGGGT CTTACCTCGT GACGTCTGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525530.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525593.1 Mus musculus adenomatosis polyposis coli (Apc), transcript variant X2, mRNA.

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CloneID	OMu54318
Clone ID Related Accession (Same CDS sequence)	NM_001360980.1 , XM_006525530.1 , XM_006525531.1 , XM_006525530.2
Accession Version	XM_006525530.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGTAGGAAG GCTACCCGGG GCATTTCATC CCAGGAGCTA 
GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC ATTGCAGAGT TATTGCAGGT GGACTGTGAG 
ATGTATGGGC TTACTAATGA CCACTACAGT GTTACTTTAA GACGGTATGC TGGAATGGCT 
TTGACAAACT TGACCTTTGG AGATGTTGCC AACAAGGCTA CGCTGTGTTC TATGAAAGGC 
TGCATGAGAG CACTTGTGGC CCAGTTAAAA TCTGAGAGTG AAGACTTACA GCAGGTTATT 
GCAAGTGTTT TGAGGAATTT GTCTTGGCGA GCAGATGTAA ATAGCAAAAA GACGTTGAGA 
GAAGTTGGAA GTGTGAAAGC ATTGATGGAA TGTGCTTTGG AAGTTAAAAA GGAATCAACC 
CTCAAAAGCG TTTTGAGTGC CTTATGGAAC CTGTCTGCAC ACTGCACTGA GAATAAGGCT 
GACATCTGTG CTGTGGATGG AGCACTGGCA TTTCTGGTTG GCACCCTCAC TTACCGGAGC 
CAGACAAATA CTTTAGCCAT TATTGAAAGT GGAGGTGGGA TATTACGGAA TGTGTCCAGC 
TTGATAGCTA CAAACGAAGA CCACAGGCAA ATCCTAAGAG AGAACAATTG CCTACAAACT 
TTATTACAGC ACTTGAAATC TCACAGCTTG ACAATAGTCA GTAATGCATG TGGAACTTTG 
TGGAATCTCT CAGCAAGAAA TCCTAAAGAC CAGGAAGCCT TGTGGGACAT GGGGGCAGTG 
AGCATGCTCA AGAACCTCAT TCATTCCAAG CACAAAATGA TTGCCATGGG AAGTGCAGCA 
GCTTTAAGGA ATCTCATGGC AAACAGACCT GCAAAGTATA AGGATGCCAA TATCATGTCT 
CCCGGCTCAA GTCTGCCATC CCTTCACGTT AGGAAACAGA AAGCTCTAGA AGCTGAGCTA 
GATGCTCAGC ATTTATCAGA AACCTTCGAC AACATTGACA ACCTAAGTCC CAAGGCCTCT 
CACCGGAGTA AGCAGAGACA CAAGCAGAAT CTTTATGGTG ACTATGCTTT TGACGCCAAT 
CGACATGATG ATAGTAGGTC AGACAATTTC AATACTGGAA ACATGACTGT TCTTTCACCA 
TATTTAAATA CTACGGTATT GCCCAGCTCT TCTTCCTCAA GGGGAAGTTT AGACAGTTCT 
CGTTCTGAGA AAGACAGAAG TTTGGAGAGA GAGCGAGGTA TTGGCCTCAG TGCTTACCAT 
CCAACAACAG AAAATGCAGG AACCTCATCA AAACGAGGTC TGCAGATCAC TACCACTGCA 
GCCCAGATAG CCAAAGTTAT GGAAGAAGTA TCAGCCATTC ATACCTCCCA GGACGACAGA 
AGTTCTGCTT CTACCACCGA GTTCCATTGT GTGGCAGACG ACAGGAGTGC GGCACGAAGA 
AGCTCTGCCT CCCACACACA CTCAAACACA TACAACTTCA CTAAGTCGGA AAATTCAAAT 
AGGACATGCT CTATGCCTTA TGCCAAAGTG GAATATAAAC GATCTTCAAA TGACAGTTTA 
AATAGTGTCA CTAGTAGTGA TGGATATGGT AAAAGAGGCC AAATGAAACC CTCAGTTGAA 
TCCTATTCTG AAGATGATGA AAGTAAATTT TGCAGTTATG GTCAGTATCC AGCTGACCTA 
GCCCATAAGA TACACAGTGC AAATCATATG GATGATAATG ATGGAGAACT GGATACACCA 
ATAAATTACA GTCTTAAATA TTCAGATGAG CAGTTGAACT CAGGAAGGCA GAGTCCCTCA 
CAGAATGAAA GGTGGGCAAG ACCAAAGCAT GTGATAGAAG ATGAAATAAA GCAAAACGAG 
CAAAGACAAG CAAGAAGCCA GAACACCAGT TATCCTGTCT ATTCTGAGAA TACCGATGAC 
AAACACCTCA AATTCCAACC ACATTTTGGA CAACAAGAAT GTGTTTCCCC ATATAGGTCA 
AGGGGAACCA GTGGTTCAGA AACAAATCGA ATGGGTTCTA GTCATGCAAT TAATCAAAAT 
GTAAACCAGT CTCTGTGTCA GGAAGATGAT TATGAAGATG ATAAACCTAC CAACTACAGT 
GAACGTTATT CTGAGGAAGA ACAACATGAA GAAGAAGAAG AGAGACCGAC AAATTATAGC 
ATAAAATATA ATGAAGAGAA ACATCATGTG GATCAGCCTA TTGATTATAG TTTAAAATAT 
GCCACTGACA TTTCTTCCTC ACAAAAACCA TCATTTTCAT TCTCAAAGAA TTCATCAGCA 
CAAAGCACTA AACCTGAACA TCTCTCTCCA AGCAGCGAGA ATACAGCTGT ACCTCCATCT 
AATGCCAAAA GGCAGAATCA GCTGCGTCCA AGTTCAGCAC AAAGAAATGG CCAGACTCAA 
AAAGGCACTA CTTGCAAAGT CCCCTCCATC AACCAAGAAA CAATACAGAC TTACTGCGTA 
GAAGACACCC CAATATGTTT TTCAAGGTGC AGTTCATTAT CATCACTGTC ATCAGCTGAC 
GATGAAATAG GATGTGATCA GACAACACAG GAAGCAGATT CTGCTAATAC TCTGCAGACA 
GCAGAAGTAA AAGAGAATGA TGTAACTCGG TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA 
GCAGTGTCCC AGAATGCTAG AGCCAAACCC AGCCGACTCC AGGCTTCTGG CTTATCTTCA 
GAATCAACCA GGCATAATAA AGCTGTTGAG TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA 
AGTGGTGCTC AGACACCCAA AAGTCCCCCA GAACACTATG TCCAGGAGAC TCCGCTCGTA 
TTCAGCAGGT GTACTTCTGT CAGCTCCCTT GACAGTTTTG AGAGTCGCTC CATTGCCAGC 
TCTGTTCAGA GTGAGCCATG TAGTGGAATG GTGAGTGGCA TCATAAGCCC CAGTGACCTT 
CCAGATAGTC CTGGGCAGAC CATGCCACCA AGCAGAAGCA AAACCCCTCC ACCTCCTCCA 
CAGACAGTGC AGGCCAAGAG AGAGGTGCCA AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA 
GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA 
GACGTGGATA CTTTGTTACA CTTCGCCACA GAAAGTACTC CAGACGGGTT TTCTTGTTCC 
TCCAGCCTAA GTGCTCTGAG CCTGGATGAG CCATTTATAC AGAAAGATGT AGAATTAAGA 
ATCATGCCTC CAGTTCAGGA AAACGACAAT GGGAATGAAA CTGAATCAGA ACAGCCTGAG 
GAATCAAATG AAAACCAGGA TAAAGAGGTA GAAAAGCCTG ACTCTGAAAA AGACTTATTA 
GATGATTCTG ATGACGATGA TATTGAAATA TTAGAAGAAT GTATTATTTC AGCCATGCCA 
ACAAAGTCAT CACGCAAAGC CAAAAAACTA GCCCAGACTG CTTCAAAATT ACCTCCACCT 
GTGGCAAGGA AACCAAGTCA GCTACCTGTG TATAAACTTC TGCCAGCACA GAATAGGCTG 
CAGGCACAAA AACATGTTAG CTTTACACCA GGGGATGATG TGCCCCGGGT GTACTGTGTA 
GAAGGGACAC CTATAAACTT TTCCACAGCA ACGTCTCTAA GTGATCTGAC AATAGAGTCC 
CCTCCAAATG AATTGGCTAC TGGAGATGGG GTCAGAGCGG GTATACAGTC AGGTGAATTT 
GAAAAACGAG ATACCATTCC TACAGAAGGC AGAAGTACAG ATGATGCTCA GCGAGGAAAA 
ATCTCATCTA TAGTTACACC AGACCTGGAT GACAACAAAG CAGAGGAAGG AGATATTCTT 
GCAGAATGTA TCAATTCTGC TATGCCCAAA GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA 
AAGATAATGG ACCAAGTCCA ACAAGCATCC TCGACTTCAT CTGGAGCTAA CAAAAATCAA 
GTAGACACTA AGAAAAAGAA GCCTACTTCA CCAGTAAAGC CCATGCCACA AAATACTGAA 
TATAGAACGC GTGTGAGAAA GAATACAGAC TCAAAAGTTA ATGTAAATAC TGAAGAAACT 
TTCTCAGACA ACAAAGACTC AAAGAAACCA AGCTTACAAA CCAATGCCAA GGCCTTCAAT 
GAAAAGCTAC CTAACAATGA AGACAGAGTG CGGGGGAGCT TCGCCTTGGA CTCACCGCAT 
CACTACACCC CTATTGAGGG GACGCCGTAC TGCTTTTCCC GAAATGACTC CTTGAGTTCT 
CTGGATTTTG ATGATGACGA TGTTGACCTT TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC 
AAAGAAAGCA AGGATTCCGA AGCCAAAGTT ACCTGCCGCC CAGAACCAAA CTCAAGCCAG 
CAGGCAGCTA GTAAGTCACA AGCCAGTATA AAACATCCAG CAAACAGAGC ACAGTCCAAA 
CCAGTGCTGC AGAAACAGCC CACTTTCCCC CAGTCCTCCA AAGACGGACC AGATAGAGGG 
GCAGCAACTG ACGAAAAACT GCAGAATTTT GCTATTGAAA ATACTCCAGT TTGCTTTTCT 
CGAAATTCCT CTCTGAGTTC CCTTAGTGAC ATTGACCAGG AAAACAACAA TAACAAAGAA 
AGTGAACCAA TCAAAGAAGC TGAACCTGCC AACTCACAAG GAGAGCCCAG TAAGCCTCAG 
GCATCCGGGT ATGCTCCCAA GTCCTTCCAC GTCGAAGACA CCCCTGTCTG TTTCTCAAGA 
AACAGCTCTC TCAGTTCTCT TAGCATTGAC TCTGAGGACG ACCTGTTACA GGAGTGTATA 
AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT TCAAGACTCA AGAGTGAGAG CGAAAAGCAG 
AGCCCTAGAA AAGTGGGTGG CATATTAGCT GAAGACCTGA CGCTTGATTT GAAAGATCTA 
CAGAGGCCAG ATTCAGAACA CGCTTTCTCC CCCGACTCAG AAAATTTTGA CTGGAAAGCT 
ATTCAGGAAG GCGCAAACTC CATAGTAAGT AGTTTGCACC AAGCTGCTGC AGCCGCCGCG 
TGCTTATCTA GACAAGCGTC ATCCGACTCA GATTCCATTC TGTCACTAAA GTCCGGCATT 
TCTCTGGGAT CGCCTTTTCA TCTTACACCT GATCAAGAGG AAAAGCCATT CACAAGCAAT 
AAAGGCCCAA GAATTCTCAA ACCTGGAGAG AAAAGCACAT TAGAAGCAAA AAAAATAGAA 
TCTGAAAACA AAGGAATCAA AGGCGGGAAA AAGGTTTATA AAAGCTTGAT TACGGGAAAG 
ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA ATGAAACAAC CCCTCCCGAC AAACATGCCT 
TCAATCTCAA GAGGCAGGAC GATGATTCAC ATCCCAGGGC TTCGGAATAG CTCCTCTAGT 
ACAAGCCCTG TCTCTAAGAA AGGCCCACCC CTCAAGACTC CAGCCTCTAA AAGCCCCAGT 
GAAGGGCCGG GAGCTACCAC TTCTCCTCGA GGAACTAAGC CAGCAGGAAA GTCAGAGCTT 
AGCCCTATCA CCAGGCAAAC TTCCCAAATC AGTGGGTCAA ATAAGGGGTC TTCTAGATCA 
GGATCTAGAG ACTCCACTCC CTCAAGACCT ACACAGCAAC CATTAAGTAG GCCAATGCAG 
TCTCCAGGGC GAAACTCAAT TTCCCCTGGT AGAAATGGAA TAAGCCCTCC TAACAAACTG 
TCTCAGCTGC CCAGAACATC ATCTCCCAGT ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG 
AAAATGTCAT ATACATCCCC AGGTAGACAG CTGAGCCAAC AAAATCTTAC CAAACAAGCA 
AGTTTATCCA AGAATGCCAG CAGTATCCCC AGAAGTGAGT CGGCATCTAA AGGACTGAAT 
CAGATGAGTA ACGGCAATGG GTCAAATAAA AAGGTAGAAC TTTCTAGAAT GTCTTCAACT 
AAATCAAGTG GAAGTGAATC AGACAGATCA GAAAGGCCTG CATTAGTACG CCAGTCTACT 
TTCATCAAAG AAGCCCCAAG CCCAACCCTG AGGAGGAAAC TGGAGGAATC TGCCTCATTT 
GAATCCCTTT CTCCATCTTC TAGACCAGAT TCTCCCACCA GGTCGCAGGC ACAGACCCCA 
GTTTTAAGCC CTTCCCTTCC TGATATGTCT CTGTCCACAC ATCCATCTGT TCAGGCAGGT 
GGGTGGCGAA AGCTCCCGCC TAATCTCAGC CCCACTATCG AGTATAATGA CGGAAGGCCC 
ACAAAACGGC ATGATATTGC ACGCTCCCAT TCTGAAAGTC CTTCCAGACT ACCAATCAAC 
CGGGCGGGAA CCTGGAAGCG TGAACACAGC AAACATTCCT CGTCCCTTCC TCGAGTGAGT 
ACTTGGAGAA GAACTGGAAG CTCATCTTCT ATTCTTTCTG CTTCATCAGA GTCCAGTGAA 
AAAGCAAAAA GTGAGGATGA AAGGCATGTG AGCTCCATGC CAGCACCCAG ACAGATGAAG 
GAAAACCAGG TGCCCACCAA AGGAACATGG AGGAAAATCA AGGAAAGTGA CATTTCTCCC 
ACAGGCATGG CTTCTCAGAG CGCTTCCTCA GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT 
CTGATCTATC AGATGGCACC TCCTGTCTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG 
GACTGCCCCA TTAACAACCC TAGATCTGGA CGGTCCCCCA CAGGCAACAC CCCCCCAGTG 
ATTGACAGTG TTTCAGAGAA GGGAAGTTCA AGCATTAAAG ATTCAAAAGA CACCCATGGG 
AAACAGAGTG TGGGCAGTGG CAGTCCTGTG CAAACCGTGG GTCTGGAAAC CCGCCTCAAC 
TCCTTTGTTC AGGTAGAGGC CCCAGAACAG AAAGGAACTG AGGCAAAACC AGGACAGAGT 
AACCCAGTCT CTATAGCAGA GACTGCTGAG ACGTGTATAG CAGAGCGTAC CCCTTTCAGT 
TCCAGTAGCT CCAGCAAGCA CAGCTCACCT AGCGGGACTG TTGCTGCCAG AGTGACACCT 
TTTAATTACA ACCCTAGCCC TAGGAAGAGC AGCGCAGACA GCACTTCAGC CCGGCCGTCT 
CAGATCCCTA CGCCAGTGAG CACCAACACG AAGAAGAGAG ATTCGAAGAC TGACAGCACA 
GAATCCAGTG GAGCCCAAAG TCCTAAACGC CATTCCGGGT CTTACCTCGT GACGTCTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525593.1
CDS	312..8894
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525530.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525530.1

ORF Insert Sequence:

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGTAGGAAG GCTACCCGGG GCATTTCATC CCAGGAGCTA 
GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC ATTGCAGAGT TATTGCAGGT GGACTGTGAG 
ATGTATGGGC TTACTAATGA CCACTACAGT GTTACTTTAA GACGGTATGC TGGAATGGCT 
TTGACAAACT TGACCTTTGG AGATGTTGCC AACAAGGCTA CGCTGTGTTC TATGAAAGGC 
TGCATGAGAG CACTTGTGGC CCAGTTAAAA TCTGAGAGTG AAGACTTACA GCAGGTTATT 
GCAAGTGTTT TGAGGAATTT GTCTTGGCGA GCAGATGTAA ATAGCAAAAA GACGTTGAGA 
GAAGTTGGAA GTGTGAAAGC ATTGATGGAA TGTGCTTTGG AAGTTAAAAA GGAATCAACC 
CTCAAAAGCG TTTTGAGTGC CTTATGGAAC CTGTCTGCAC ACTGCACTGA GAATAAGGCT 
GACATCTGTG CTGTGGATGG AGCACTGGCA TTTCTGGTTG GCACCCTCAC TTACCGGAGC 
CAGACAAATA CTTTAGCCAT TATTGAAAGT GGAGGTGGGA TATTACGGAA TGTGTCCAGC 
TTGATAGCTA CAAACGAAGA CCACAGGCAA ATCCTAAGAG AGAACAATTG CCTACAAACT 
TTATTACAGC ACTTGAAATC TCACAGCTTG ACAATAGTCA GTAATGCATG TGGAACTTTG 
TGGAATCTCT CAGCAAGAAA TCCTAAAGAC CAGGAAGCCT TGTGGGACAT GGGGGCAGTG 
AGCATGCTCA AGAACCTCAT TCATTCCAAG CACAAAATGA TTGCCATGGG AAGTGCAGCA 
GCTTTAAGGA ATCTCATGGC AAACAGACCT GCAAAGTATA AGGATGCCAA TATCATGTCT 
CCCGGCTCAA GTCTGCCATC CCTTCACGTT AGGAAACAGA AAGCTCTAGA AGCTGAGCTA 
GATGCTCAGC ATTTATCAGA AACCTTCGAC AACATTGACA ACCTAAGTCC CAAGGCCTCT 
CACCGGAGTA AGCAGAGACA CAAGCAGAAT CTTTATGGTG ACTATGCTTT TGACGCCAAT 
CGACATGATG ATAGTAGGTC AGACAATTTC AATACTGGAA ACATGACTGT TCTTTCACCA 
TATTTAAATA CTACGGTATT GCCCAGCTCT TCTTCCTCAA GGGGAAGTTT AGACAGTTCT 
CGTTCTGAGA AAGACAGAAG TTTGGAGAGA GAGCGAGGTA TTGGCCTCAG TGCTTACCAT 
CCAACAACAG AAAATGCAGG AACCTCATCA AAACGAGGTC TGCAGATCAC TACCACTGCA 
GCCCAGATAG CCAAAGTTAT GGAAGAAGTA TCAGCCATTC ATACCTCCCA GGACGACAGA 
AGTTCTGCTT CTACCACCGA GTTCCATTGT GTGGCAGACG ACAGGAGTGC GGCACGAAGA 
AGCTCTGCCT CCCACACACA CTCAAACACA TACAACTTCA CTAAGTCGGA AAATTCAAAT 
AGGACATGCT CTATGCCTTA TGCCAAAGTG GAATATAAAC GATCTTCAAA TGACAGTTTA 
AATAGTGTCA CTAGTAGTGA TGGATATGGT AAAAGAGGCC AAATGAAACC CTCAGTTGAA 
TCCTATTCTG AAGATGATGA AAGTAAATTT TGCAGTTATG GTCAGTATCC AGCTGACCTA 
GCCCATAAGA TACACAGTGC AAATCATATG GATGATAATG ATGGAGAACT GGATACACCA 
ATAAATTACA GTCTTAAATA TTCAGATGAG CAGTTGAACT CAGGAAGGCA GAGTCCCTCA 
CAGAATGAAA GGTGGGCAAG ACCAAAGCAT GTGATAGAAG ATGAAATAAA GCAAAACGAG 
CAAAGACAAG CAAGAAGCCA GAACACCAGT TATCCTGTCT ATTCTGAGAA TACCGATGAC 
AAACACCTCA AATTCCAACC ACATTTTGGA CAACAAGAAT GTGTTTCCCC ATATAGGTCA 
AGGGGAACCA GTGGTTCAGA AACAAATCGA ATGGGTTCTA GTCATGCAAT TAATCAAAAT 
GTAAACCAGT CTCTGTGTCA GGAAGATGAT TATGAAGATG ATAAACCTAC CAACTACAGT 
GAACGTTATT CTGAGGAAGA ACAACATGAA GAAGAAGAAG AGAGACCGAC AAATTATAGC 
ATAAAATATA ATGAAGAGAA ACATCATGTG GATCAGCCTA TTGATTATAG TTTAAAATAT 
GCCACTGACA TTTCTTCCTC ACAAAAACCA TCATTTTCAT TCTCAAAGAA TTCATCAGCA 
CAAAGCACTA AACCTGAACA TCTCTCTCCA AGCAGCGAGA ATACAGCTGT ACCTCCATCT 
AATGCCAAAA GGCAGAATCA GCTGCGTCCA AGTTCAGCAC AAAGAAATGG CCAGACTCAA 
AAAGGCACTA CTTGCAAAGT CCCCTCCATC AACCAAGAAA CAATACAGAC TTACTGCGTA 
GAAGACACCC CAATATGTTT TTCAAGGTGC AGTTCATTAT CATCACTGTC ATCAGCTGAC 
GATGAAATAG GATGTGATCA GACAACACAG GAAGCAGATT CTGCTAATAC TCTGCAGACA 
GCAGAAGTAA AAGAGAATGA TGTAACTCGG TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA 
GCAGTGTCCC AGAATGCTAG AGCCAAACCC AGCCGACTCC AGGCTTCTGG CTTATCTTCA 
GAATCAACCA GGCATAATAA AGCTGTTGAG TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA 
AGTGGTGCTC AGACACCCAA AAGTCCCCCA GAACACTATG TCCAGGAGAC TCCGCTCGTA 
TTCAGCAGGT GTACTTCTGT CAGCTCCCTT GACAGTTTTG AGAGTCGCTC CATTGCCAGC 
TCTGTTCAGA GTGAGCCATG TAGTGGAATG GTGAGTGGCA TCATAAGCCC CAGTGACCTT 
CCAGATAGTC CTGGGCAGAC CATGCCACCA AGCAGAAGCA AAACCCCTCC ACCTCCTCCA 
CAGACAGTGC AGGCCAAGAG AGAGGTGCCA AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA 
GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA 
GACGTGGATA CTTTGTTACA CTTCGCCACA GAAAGTACTC CAGACGGGTT TTCTTGTTCC 
TCCAGCCTAA GTGCTCTGAG CCTGGATGAG CCATTTATAC AGAAAGATGT AGAATTAAGA 
ATCATGCCTC CAGTTCAGGA AAACGACAAT GGGAATGAAA CTGAATCAGA ACAGCCTGAG 
GAATCAAATG AAAACCAGGA TAAAGAGGTA GAAAAGCCTG ACTCTGAAAA AGACTTATTA 
GATGATTCTG ATGACGATGA TATTGAAATA TTAGAAGAAT GTATTATTTC AGCCATGCCA 
ACAAAGTCAT CACGCAAAGC CAAAAAACTA GCCCAGACTG CTTCAAAATT ACCTCCACCT 
GTGGCAAGGA AACCAAGTCA GCTACCTGTG TATAAACTTC TGCCAGCACA GAATAGGCTG 
CAGGCACAAA AACATGTTAG CTTTACACCA GGGGATGATG TGCCCCGGGT GTACTGTGTA 
GAAGGGACAC CTATAAACTT TTCCACAGCA ACGTCTCTAA GTGATCTGAC AATAGAGTCC 
CCTCCAAATG AATTGGCTAC TGGAGATGGG GTCAGAGCGG GTATACAGTC AGGTGAATTT 
GAAAAACGAG ATACCATTCC TACAGAAGGC AGAAGTACAG ATGATGCTCA GCGAGGAAAA 
ATCTCATCTA TAGTTACACC AGACCTGGAT GACAACAAAG CAGAGGAAGG AGATATTCTT 
GCAGAATGTA TCAATTCTGC TATGCCCAAA GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA 
AAGATAATGG ACCAAGTCCA ACAAGCATCC TCGACTTCAT CTGGAGCTAA CAAAAATCAA 
GTAGACACTA AGAAAAAGAA GCCTACTTCA CCAGTAAAGC CCATGCCACA AAATACTGAA 
TATAGAACGC GTGTGAGAAA GAATACAGAC TCAAAAGTTA ATGTAAATAC TGAAGAAACT 
TTCTCAGACA ACAAAGACTC AAAGAAACCA AGCTTACAAA CCAATGCCAA GGCCTTCAAT 
GAAAAGCTAC CTAACAATGA AGACAGAGTG CGGGGGAGCT TCGCCTTGGA CTCACCGCAT 
CACTACACCC CTATTGAGGG GACGCCGTAC TGCTTTTCCC GAAATGACTC CTTGAGTTCT 
CTGGATTTTG ATGATGACGA TGTTGACCTT TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC 
AAAGAAAGCA AGGATTCCGA AGCCAAAGTT ACCTGCCGCC CAGAACCAAA CTCAAGCCAG 
CAGGCAGCTA GTAAGTCACA AGCCAGTATA AAACATCCAG CAAACAGAGC ACAGTCCAAA 
CCAGTGCTGC AGAAACAGCC CACTTTCCCC CAGTCCTCCA AAGACGGACC AGATAGAGGG 
GCAGCAACTG ACGAAAAACT GCAGAATTTT GCTATTGAAA ATACTCCAGT TTGCTTTTCT 
CGAAATTCCT CTCTGAGTTC CCTTAGTGAC ATTGACCAGG AAAACAACAA TAACAAAGAA 
AGTGAACCAA TCAAAGAAGC TGAACCTGCC AACTCACAAG GAGAGCCCAG TAAGCCTCAG 
GCATCCGGGT ATGCTCCCAA GTCCTTCCAC GTCGAAGACA CCCCTGTCTG TTTCTCAAGA 
AACAGCTCTC TCAGTTCTCT TAGCATTGAC TCTGAGGACG ACCTGTTACA GGAGTGTATA 
AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT TCAAGACTCA AGAGTGAGAG CGAAAAGCAG 
AGCCCTAGAA AAGTGGGTGG CATATTAGCT GAAGACCTGA CGCTTGATTT GAAAGATCTA 
CAGAGGCCAG ATTCAGAACA CGCTTTCTCC CCCGACTCAG AAAATTTTGA CTGGAAAGCT 
ATTCAGGAAG GCGCAAACTC CATAGTAAGT AGTTTGCACC AAGCTGCTGC AGCCGCCGCG 
TGCTTATCTA GACAAGCGTC ATCCGACTCA GATTCCATTC TGTCACTAAA GTCCGGCATT 
TCTCTGGGAT CGCCTTTTCA TCTTACACCT GATCAAGAGG AAAAGCCATT CACAAGCAAT 
AAAGGCCCAA GAATTCTCAA ACCTGGAGAG AAAAGCACAT TAGAAGCAAA AAAAATAGAA 
TCTGAAAACA AAGGAATCAA AGGCGGGAAA AAGGTTTATA AAAGCTTGAT TACGGGAAAG 
ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA ATGAAACAAC CCCTCCCGAC AAACATGCCT 
TCAATCTCAA GAGGCAGGAC GATGATTCAC ATCCCAGGGC TTCGGAATAG CTCCTCTAGT 
ACAAGCCCTG TCTCTAAGAA AGGCCCACCC CTCAAGACTC CAGCCTCTAA AAGCCCCAGT 
GAAGGGCCGG GAGCTACCAC TTCTCCTCGA GGAACTAAGC CAGCAGGAAA GTCAGAGCTT 
AGCCCTATCA CCAGGCAAAC TTCCCAAATC AGTGGGTCAA ATAAGGGGTC TTCTAGATCA 
GGATCTAGAG ACTCCACTCC CTCAAGACCT ACACAGCAAC CATTAAGTAG GCCAATGCAG 
TCTCCAGGGC GAAACTCAAT TTCCCCTGGT AGAAATGGAA TAAGCCCTCC TAACAAACTG 
TCTCAGCTGC CCAGAACATC ATCTCCCAGT ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG 
AAAATGTCAT ATACATCCCC AGGTAGACAG CTGAGCCAAC AAAATCTTAC CAAACAAGCA 
AGTTTATCCA AGAATGCCAG CAGTATCCCC AGAAGTGAGT CGGCATCTAA AGGACTGAAT 
CAGATGAGTA ACGGCAATGG GTCAAATAAA AAGGTAGAAC TTTCTAGAAT GTCTTCAACT 
AAATCAAGTG GAAGTGAATC AGACAGATCA GAAAGGCCTG CATTAGTACG CCAGTCTACT 
TTCATCAAAG AAGCCCCAAG CCCAACCCTG AGGAGGAAAC TGGAGGAATC TGCCTCATTT 
GAATCCCTTT CTCCATCTTC TAGACCAGAT TCTCCCACCA GGTCGCAGGC ACAGACCCCA 
GTTTTAAGCC CTTCCCTTCC TGATATGTCT CTGTCCACAC ATCCATCTGT TCAGGCAGGT 
GGGTGGCGAA AGCTCCCGCC TAATCTCAGC CCCACTATCG AGTATAATGA CGGAAGGCCC 
ACAAAACGGC ATGATATTGC ACGCTCCCAT TCTGAAAGTC CTTCCAGACT ACCAATCAAC 
CGGGCGGGAA CCTGGAAGCG TGAACACAGC AAACATTCCT CGTCCCTTCC TCGAGTGAGT 
ACTTGGAGAA GAACTGGAAG CTCATCTTCT ATTCTTTCTG CTTCATCAGA GTCCAGTGAA 
AAAGCAAAAA GTGAGGATGA AAGGCATGTG AGCTCCATGC CAGCACCCAG ACAGATGAAG 
GAAAACCAGG TGCCCACCAA AGGAACATGG AGGAAAATCA AGGAAAGTGA CATTTCTCCC 
ACAGGCATGG CTTCTCAGAG CGCTTCCTCA GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT 
CTGATCTATC AGATGGCACC TCCTGTCTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG 
GACTGCCCCA TTAACAACCC TAGATCTGGA CGGTCCCCCA CAGGCAACAC CCCCCCAGTG 
ATTGACAGTG TTTCAGAGAA GGGAAGTTCA AGCATTAAAG ATTCAAAAGA CACCCATGGG 
AAACAGAGTG TGGGCAGTGG CAGTCCTGTG CAAACCGTGG GTCTGGAAAC CCGCCTCAAC 
TCCTTTGTTC AGGTAGAGGC CCCAGAACAG AAAGGAACTG AGGCAAAACC AGGACAGAGT 
AACCCAGTCT CTATAGCAGA GACTGCTGAG ACGTGTATAG CAGAGCGTAC CCCTTTCAGT 
TCCAGTAGCT CCAGCAAGCA CAGCTCACCT AGCGGGACTG TTGCTGCCAG AGTGACACCT 
TTTAATTACA ACCCTAGCCC TAGGAAGAGC AGCGCAGACA GCACTTCAGC CCGGCCGTCT 
CAGATCCCTA CGCCAGTGAG CACCAACACG AAGAAGAGAG ATTCGAAGAC TGACAGCACA 
GAATCCAGTG GAGCCCAAAG TCCTAAACGC CATTCCGGGT CTTACCTCGT GACGTCTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525531.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525594.1 Mus musculus adenomatosis polyposis coli (Apc), transcript variant X3, mRNA.

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CloneID	OMu54318
Clone ID Related Accession (Same CDS sequence)	NM_001360980.1 , XM_006525530.1 , XM_006525531.1 , XM_006525530.2
Accession Version	XM_006525531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGTAGGAAG GCTACCCGGG GCATTTCATC CCAGGAGCTA 
GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC ATTGCAGAGT TATTGCAGGT GGACTGTGAG 
ATGTATGGGC TTACTAATGA CCACTACAGT GTTACTTTAA GACGGTATGC TGGAATGGCT 
TTGACAAACT TGACCTTTGG AGATGTTGCC AACAAGGCTA CGCTGTGTTC TATGAAAGGC 
TGCATGAGAG CACTTGTGGC CCAGTTAAAA TCTGAGAGTG AAGACTTACA GCAGGTTATT 
GCAAGTGTTT TGAGGAATTT GTCTTGGCGA GCAGATGTAA ATAGCAAAAA GACGTTGAGA 
GAAGTTGGAA GTGTGAAAGC ATTGATGGAA TGTGCTTTGG AAGTTAAAAA GGAATCAACC 
CTCAAAAGCG TTTTGAGTGC CTTATGGAAC CTGTCTGCAC ACTGCACTGA GAATAAGGCT 
GACATCTGTG CTGTGGATGG AGCACTGGCA TTTCTGGTTG GCACCCTCAC TTACCGGAGC 
CAGACAAATA CTTTAGCCAT TATTGAAAGT GGAGGTGGGA TATTACGGAA TGTGTCCAGC 
TTGATAGCTA CAAACGAAGA CCACAGGCAA ATCCTAAGAG AGAACAATTG CCTACAAACT 
TTATTACAGC ACTTGAAATC TCACAGCTTG ACAATAGTCA GTAATGCATG TGGAACTTTG 
TGGAATCTCT CAGCAAGAAA TCCTAAAGAC CAGGAAGCCT TGTGGGACAT GGGGGCAGTG 
AGCATGCTCA AGAACCTCAT TCATTCCAAG CACAAAATGA TTGCCATGGG AAGTGCAGCA 
GCTTTAAGGA ATCTCATGGC AAACAGACCT GCAAAGTATA AGGATGCCAA TATCATGTCT 
CCCGGCTCAA GTCTGCCATC CCTTCACGTT AGGAAACAGA AAGCTCTAGA AGCTGAGCTA 
GATGCTCAGC ATTTATCAGA AACCTTCGAC AACATTGACA ACCTAAGTCC CAAGGCCTCT 
CACCGGAGTA AGCAGAGACA CAAGCAGAAT CTTTATGGTG ACTATGCTTT TGACGCCAAT 
CGACATGATG ATAGTAGGTC AGACAATTTC AATACTGGAA ACATGACTGT TCTTTCACCA 
TATTTAAATA CTACGGTATT GCCCAGCTCT TCTTCCTCAA GGGGAAGTTT AGACAGTTCT 
CGTTCTGAGA AAGACAGAAG TTTGGAGAGA GAGCGAGGTA TTGGCCTCAG TGCTTACCAT 
CCAACAACAG AAAATGCAGG AACCTCATCA AAACGAGGTC TGCAGATCAC TACCACTGCA 
GCCCAGATAG CCAAAGTTAT GGAAGAAGTA TCAGCCATTC ATACCTCCCA GGACGACAGA 
AGTTCTGCTT CTACCACCGA GTTCCATTGT GTGGCAGACG ACAGGAGTGC GGCACGAAGA 
AGCTCTGCCT CCCACACACA CTCAAACACA TACAACTTCA CTAAGTCGGA AAATTCAAAT 
AGGACATGCT CTATGCCTTA TGCCAAAGTG GAATATAAAC GATCTTCAAA TGACAGTTTA 
AATAGTGTCA CTAGTAGTGA TGGATATGGT AAAAGAGGCC AAATGAAACC CTCAGTTGAA 
TCCTATTCTG AAGATGATGA AAGTAAATTT TGCAGTTATG GTCAGTATCC AGCTGACCTA 
GCCCATAAGA TACACAGTGC AAATCATATG GATGATAATG ATGGAGAACT GGATACACCA 
ATAAATTACA GTCTTAAATA TTCAGATGAG CAGTTGAACT CAGGAAGGCA GAGTCCCTCA 
CAGAATGAAA GGTGGGCAAG ACCAAAGCAT GTGATAGAAG ATGAAATAAA GCAAAACGAG 
CAAAGACAAG CAAGAAGCCA GAACACCAGT TATCCTGTCT ATTCTGAGAA TACCGATGAC 
AAACACCTCA AATTCCAACC ACATTTTGGA CAACAAGAAT GTGTTTCCCC ATATAGGTCA 
AGGGGAACCA GTGGTTCAGA AACAAATCGA ATGGGTTCTA GTCATGCAAT TAATCAAAAT 
GTAAACCAGT CTCTGTGTCA GGAAGATGAT TATGAAGATG ATAAACCTAC CAACTACAGT 
GAACGTTATT CTGAGGAAGA ACAACATGAA GAAGAAGAAG AGAGACCGAC AAATTATAGC 
ATAAAATATA ATGAAGAGAA ACATCATGTG GATCAGCCTA TTGATTATAG TTTAAAATAT 
GCCACTGACA TTTCTTCCTC ACAAAAACCA TCATTTTCAT TCTCAAAGAA TTCATCAGCA 
CAAAGCACTA AACCTGAACA TCTCTCTCCA AGCAGCGAGA ATACAGCTGT ACCTCCATCT 
AATGCCAAAA GGCAGAATCA GCTGCGTCCA AGTTCAGCAC AAAGAAATGG CCAGACTCAA 
AAAGGCACTA CTTGCAAAGT CCCCTCCATC AACCAAGAAA CAATACAGAC TTACTGCGTA 
GAAGACACCC CAATATGTTT TTCAAGGTGC AGTTCATTAT CATCACTGTC ATCAGCTGAC 
GATGAAATAG GATGTGATCA GACAACACAG GAAGCAGATT CTGCTAATAC TCTGCAGACA 
GCAGAAGTAA AAGAGAATGA TGTAACTCGG TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA 
GCAGTGTCCC AGAATGCTAG AGCCAAACCC AGCCGACTCC AGGCTTCTGG CTTATCTTCA 
GAATCAACCA GGCATAATAA AGCTGTTGAG TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA 
AGTGGTGCTC AGACACCCAA AAGTCCCCCA GAACACTATG TCCAGGAGAC TCCGCTCGTA 
TTCAGCAGGT GTACTTCTGT CAGCTCCCTT GACAGTTTTG AGAGTCGCTC CATTGCCAGC 
TCTGTTCAGA GTGAGCCATG TAGTGGAATG GTGAGTGGCA TCATAAGCCC CAGTGACCTT 
CCAGATAGTC CTGGGCAGAC CATGCCACCA AGCAGAAGCA AAACCCCTCC ACCTCCTCCA 
CAGACAGTGC AGGCCAAGAG AGAGGTGCCA AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA 
GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA 
GACGTGGATA CTTTGTTACA CTTCGCCACA GAAAGTACTC CAGACGGGTT TTCTTGTTCC 
TCCAGCCTAA GTGCTCTGAG CCTGGATGAG CCATTTATAC AGAAAGATGT AGAATTAAGA 
ATCATGCCTC CAGTTCAGGA AAACGACAAT GGGAATGAAA CTGAATCAGA ACAGCCTGAG 
GAATCAAATG AAAACCAGGA TAAAGAGGTA GAAAAGCCTG ACTCTGAAAA AGACTTATTA 
GATGATTCTG ATGACGATGA TATTGAAATA TTAGAAGAAT GTATTATTTC AGCCATGCCA 
ACAAAGTCAT CACGCAAAGC CAAAAAACTA GCCCAGACTG CTTCAAAATT ACCTCCACCT 
GTGGCAAGGA AACCAAGTCA GCTACCTGTG TATAAACTTC TGCCAGCACA GAATAGGCTG 
CAGGCACAAA AACATGTTAG CTTTACACCA GGGGATGATG TGCCCCGGGT GTACTGTGTA 
GAAGGGACAC CTATAAACTT TTCCACAGCA ACGTCTCTAA GTGATCTGAC AATAGAGTCC 
CCTCCAAATG AATTGGCTAC TGGAGATGGG GTCAGAGCGG GTATACAGTC AGGTGAATTT 
GAAAAACGAG ATACCATTCC TACAGAAGGC AGAAGTACAG ATGATGCTCA GCGAGGAAAA 
ATCTCATCTA TAGTTACACC AGACCTGGAT GACAACAAAG CAGAGGAAGG AGATATTCTT 
GCAGAATGTA TCAATTCTGC TATGCCCAAA GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA 
AAGATAATGG ACCAAGTCCA ACAAGCATCC TCGACTTCAT CTGGAGCTAA CAAAAATCAA 
GTAGACACTA AGAAAAAGAA GCCTACTTCA CCAGTAAAGC CCATGCCACA AAATACTGAA 
TATAGAACGC GTGTGAGAAA GAATACAGAC TCAAAAGTTA ATGTAAATAC TGAAGAAACT 
TTCTCAGACA ACAAAGACTC AAAGAAACCA AGCTTACAAA CCAATGCCAA GGCCTTCAAT 
GAAAAGCTAC CTAACAATGA AGACAGAGTG CGGGGGAGCT TCGCCTTGGA CTCACCGCAT 
CACTACACCC CTATTGAGGG GACGCCGTAC TGCTTTTCCC GAAATGACTC CTTGAGTTCT 
CTGGATTTTG ATGATGACGA TGTTGACCTT TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC 
AAAGAAAGCA AGGATTCCGA AGCCAAAGTT ACCTGCCGCC CAGAACCAAA CTCAAGCCAG 
CAGGCAGCTA GTAAGTCACA AGCCAGTATA AAACATCCAG CAAACAGAGC ACAGTCCAAA 
CCAGTGCTGC AGAAACAGCC CACTTTCCCC CAGTCCTCCA AAGACGGACC AGATAGAGGG 
GCAGCAACTG ACGAAAAACT GCAGAATTTT GCTATTGAAA ATACTCCAGT TTGCTTTTCT 
CGAAATTCCT CTCTGAGTTC CCTTAGTGAC ATTGACCAGG AAAACAACAA TAACAAAGAA 
AGTGAACCAA TCAAAGAAGC TGAACCTGCC AACTCACAAG GAGAGCCCAG TAAGCCTCAG 
GCATCCGGGT ATGCTCCCAA GTCCTTCCAC GTCGAAGACA CCCCTGTCTG TTTCTCAAGA 
AACAGCTCTC TCAGTTCTCT TAGCATTGAC TCTGAGGACG ACCTGTTACA GGAGTGTATA 
AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT TCAAGACTCA AGAGTGAGAG CGAAAAGCAG 
AGCCCTAGAA AAGTGGGTGG CATATTAGCT GAAGACCTGA CGCTTGATTT GAAAGATCTA 
CAGAGGCCAG ATTCAGAACA CGCTTTCTCC CCCGACTCAG AAAATTTTGA CTGGAAAGCT 
ATTCAGGAAG GCGCAAACTC CATAGTAAGT AGTTTGCACC AAGCTGCTGC AGCCGCCGCG 
TGCTTATCTA GACAAGCGTC ATCCGACTCA GATTCCATTC TGTCACTAAA GTCCGGCATT 
TCTCTGGGAT CGCCTTTTCA TCTTACACCT GATCAAGAGG AAAAGCCATT CACAAGCAAT 
AAAGGCCCAA GAATTCTCAA ACCTGGAGAG AAAAGCACAT TAGAAGCAAA AAAAATAGAA 
TCTGAAAACA AAGGAATCAA AGGCGGGAAA AAGGTTTATA AAAGCTTGAT TACGGGAAAG 
ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA ATGAAACAAC CCCTCCCGAC AAACATGCCT 
TCAATCTCAA GAGGCAGGAC GATGATTCAC ATCCCAGGGC TTCGGAATAG CTCCTCTAGT 
ACAAGCCCTG TCTCTAAGAA AGGCCCACCC CTCAAGACTC CAGCCTCTAA AAGCCCCAGT 
GAAGGGCCGG GAGCTACCAC TTCTCCTCGA GGAACTAAGC CAGCAGGAAA GTCAGAGCTT 
AGCCCTATCA CCAGGCAAAC TTCCCAAATC AGTGGGTCAA ATAAGGGGTC TTCTAGATCA 
GGATCTAGAG ACTCCACTCC CTCAAGACCT ACACAGCAAC CATTAAGTAG GCCAATGCAG 
TCTCCAGGGC GAAACTCAAT TTCCCCTGGT AGAAATGGAA TAAGCCCTCC TAACAAACTG 
TCTCAGCTGC CCAGAACATC ATCTCCCAGT ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG 
AAAATGTCAT ATACATCCCC AGGTAGACAG CTGAGCCAAC AAAATCTTAC CAAACAAGCA 
AGTTTATCCA AGAATGCCAG CAGTATCCCC AGAAGTGAGT CGGCATCTAA AGGACTGAAT 
CAGATGAGTA ACGGCAATGG GTCAAATAAA AAGGTAGAAC TTTCTAGAAT GTCTTCAACT 
AAATCAAGTG GAAGTGAATC AGACAGATCA GAAAGGCCTG CATTAGTACG CCAGTCTACT 
TTCATCAAAG AAGCCCCAAG CCCAACCCTG AGGAGGAAAC TGGAGGAATC TGCCTCATTT 
GAATCCCTTT CTCCATCTTC TAGACCAGAT TCTCCCACCA GGTCGCAGGC ACAGACCCCA 
GTTTTAAGCC CTTCCCTTCC TGATATGTCT CTGTCCACAC ATCCATCTGT TCAGGCAGGT 
GGGTGGCGAA AGCTCCCGCC TAATCTCAGC CCCACTATCG AGTATAATGA CGGAAGGCCC 
ACAAAACGGC ATGATATTGC ACGCTCCCAT TCTGAAAGTC CTTCCAGACT ACCAATCAAC 
CGGGCGGGAA CCTGGAAGCG TGAACACAGC AAACATTCCT CGTCCCTTCC TCGAGTGAGT 
ACTTGGAGAA GAACTGGAAG CTCATCTTCT ATTCTTTCTG CTTCATCAGA GTCCAGTGAA 
AAAGCAAAAA GTGAGGATGA AAGGCATGTG AGCTCCATGC CAGCACCCAG ACAGATGAAG 
GAAAACCAGG TGCCCACCAA AGGAACATGG AGGAAAATCA AGGAAAGTGA CATTTCTCCC 
ACAGGCATGG CTTCTCAGAG CGCTTCCTCA GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT 
CTGATCTATC AGATGGCACC TCCTGTCTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG 
GACTGCCCCA TTAACAACCC TAGATCTGGA CGGTCCCCCA CAGGCAACAC CCCCCCAGTG 
ATTGACAGTG TTTCAGAGAA GGGAAGTTCA AGCATTAAAG ATTCAAAAGA CACCCATGGG 
AAACAGAGTG TGGGCAGTGG CAGTCCTGTG CAAACCGTGG GTCTGGAAAC CCGCCTCAAC 
TCCTTTGTTC AGGTAGAGGC CCCAGAACAG AAAGGAACTG AGGCAAAACC AGGACAGAGT 
AACCCAGTCT CTATAGCAGA GACTGCTGAG ACGTGTATAG CAGAGCGTAC CCCTTTCAGT 
TCCAGTAGCT CCAGCAAGCA CAGCTCACCT AGCGGGACTG TTGCTGCCAG AGTGACACCT 
TTTAATTACA ACCCTAGCCC TAGGAAGAGC AGCGCAGACA GCACTTCAGC CCGGCCGTCT 
CAGATCCCTA CGCCAGTGAG CACCAACACG AAGAAGAGAG ATTCGAAGAC TGACAGCACA 
GAATCCAGTG GAGCCCAAAG TCCTAAACGC CATTCCGGGT CTTACCTCGT GACGTCTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525594.1
CDS	99..8681
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525531.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525531.1

ORF Insert Sequence:

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGTAGGAAG GCTACCCGGG GCATTTCATC CCAGGAGCTA 
GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC ATTGCAGAGT TATTGCAGGT GGACTGTGAG 
ATGTATGGGC TTACTAATGA CCACTACAGT GTTACTTTAA GACGGTATGC TGGAATGGCT 
TTGACAAACT TGACCTTTGG AGATGTTGCC AACAAGGCTA CGCTGTGTTC TATGAAAGGC 
TGCATGAGAG CACTTGTGGC CCAGTTAAAA TCTGAGAGTG AAGACTTACA GCAGGTTATT 
GCAAGTGTTT TGAGGAATTT GTCTTGGCGA GCAGATGTAA ATAGCAAAAA GACGTTGAGA 
GAAGTTGGAA GTGTGAAAGC ATTGATGGAA TGTGCTTTGG AAGTTAAAAA GGAATCAACC 
CTCAAAAGCG TTTTGAGTGC CTTATGGAAC CTGTCTGCAC ACTGCACTGA GAATAAGGCT 
GACATCTGTG CTGTGGATGG AGCACTGGCA TTTCTGGTTG GCACCCTCAC TTACCGGAGC 
CAGACAAATA CTTTAGCCAT TATTGAAAGT GGAGGTGGGA TATTACGGAA TGTGTCCAGC 
TTGATAGCTA CAAACGAAGA CCACAGGCAA ATCCTAAGAG AGAACAATTG CCTACAAACT 
TTATTACAGC ACTTGAAATC TCACAGCTTG ACAATAGTCA GTAATGCATG TGGAACTTTG 
TGGAATCTCT CAGCAAGAAA TCCTAAAGAC CAGGAAGCCT TGTGGGACAT GGGGGCAGTG 
AGCATGCTCA AGAACCTCAT TCATTCCAAG CACAAAATGA TTGCCATGGG AAGTGCAGCA 
GCTTTAAGGA ATCTCATGGC AAACAGACCT GCAAAGTATA AGGATGCCAA TATCATGTCT 
CCCGGCTCAA GTCTGCCATC CCTTCACGTT AGGAAACAGA AAGCTCTAGA AGCTGAGCTA 
GATGCTCAGC ATTTATCAGA AACCTTCGAC AACATTGACA ACCTAAGTCC CAAGGCCTCT 
CACCGGAGTA AGCAGAGACA CAAGCAGAAT CTTTATGGTG ACTATGCTTT TGACGCCAAT 
CGACATGATG ATAGTAGGTC AGACAATTTC AATACTGGAA ACATGACTGT TCTTTCACCA 
TATTTAAATA CTACGGTATT GCCCAGCTCT TCTTCCTCAA GGGGAAGTTT AGACAGTTCT 
CGTTCTGAGA AAGACAGAAG TTTGGAGAGA GAGCGAGGTA TTGGCCTCAG TGCTTACCAT 
CCAACAACAG AAAATGCAGG AACCTCATCA AAACGAGGTC TGCAGATCAC TACCACTGCA 
GCCCAGATAG CCAAAGTTAT GGAAGAAGTA TCAGCCATTC ATACCTCCCA GGACGACAGA 
AGTTCTGCTT CTACCACCGA GTTCCATTGT GTGGCAGACG ACAGGAGTGC GGCACGAAGA 
AGCTCTGCCT CCCACACACA CTCAAACACA TACAACTTCA CTAAGTCGGA AAATTCAAAT 
AGGACATGCT CTATGCCTTA TGCCAAAGTG GAATATAAAC GATCTTCAAA TGACAGTTTA 
AATAGTGTCA CTAGTAGTGA TGGATATGGT AAAAGAGGCC AAATGAAACC CTCAGTTGAA 
TCCTATTCTG AAGATGATGA AAGTAAATTT TGCAGTTATG GTCAGTATCC AGCTGACCTA 
GCCCATAAGA TACACAGTGC AAATCATATG GATGATAATG ATGGAGAACT GGATACACCA 
ATAAATTACA GTCTTAAATA TTCAGATGAG CAGTTGAACT CAGGAAGGCA GAGTCCCTCA 
CAGAATGAAA GGTGGGCAAG ACCAAAGCAT GTGATAGAAG ATGAAATAAA GCAAAACGAG 
CAAAGACAAG CAAGAAGCCA GAACACCAGT TATCCTGTCT ATTCTGAGAA TACCGATGAC 
AAACACCTCA AATTCCAACC ACATTTTGGA CAACAAGAAT GTGTTTCCCC ATATAGGTCA 
AGGGGAACCA GTGGTTCAGA AACAAATCGA ATGGGTTCTA GTCATGCAAT TAATCAAAAT 
GTAAACCAGT CTCTGTGTCA GGAAGATGAT TATGAAGATG ATAAACCTAC CAACTACAGT 
GAACGTTATT CTGAGGAAGA ACAACATGAA GAAGAAGAAG AGAGACCGAC AAATTATAGC 
ATAAAATATA ATGAAGAGAA ACATCATGTG GATCAGCCTA TTGATTATAG TTTAAAATAT 
GCCACTGACA TTTCTTCCTC ACAAAAACCA TCATTTTCAT TCTCAAAGAA TTCATCAGCA 
CAAAGCACTA AACCTGAACA TCTCTCTCCA AGCAGCGAGA ATACAGCTGT ACCTCCATCT 
AATGCCAAAA GGCAGAATCA GCTGCGTCCA AGTTCAGCAC AAAGAAATGG CCAGACTCAA 
AAAGGCACTA CTTGCAAAGT CCCCTCCATC AACCAAGAAA CAATACAGAC TTACTGCGTA 
GAAGACACCC CAATATGTTT TTCAAGGTGC AGTTCATTAT CATCACTGTC ATCAGCTGAC 
GATGAAATAG GATGTGATCA GACAACACAG GAAGCAGATT CTGCTAATAC TCTGCAGACA 
GCAGAAGTAA AAGAGAATGA TGTAACTCGG TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA 
GCAGTGTCCC AGAATGCTAG AGCCAAACCC AGCCGACTCC AGGCTTCTGG CTTATCTTCA 
GAATCAACCA GGCATAATAA AGCTGTTGAG TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA 
AGTGGTGCTC AGACACCCAA AAGTCCCCCA GAACACTATG TCCAGGAGAC TCCGCTCGTA 
TTCAGCAGGT GTACTTCTGT CAGCTCCCTT GACAGTTTTG AGAGTCGCTC CATTGCCAGC 
TCTGTTCAGA GTGAGCCATG TAGTGGAATG GTGAGTGGCA TCATAAGCCC CAGTGACCTT 
CCAGATAGTC CTGGGCAGAC CATGCCACCA AGCAGAAGCA AAACCCCTCC ACCTCCTCCA 
CAGACAGTGC AGGCCAAGAG AGAGGTGCCA AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA 
GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA 
GACGTGGATA CTTTGTTACA CTTCGCCACA GAAAGTACTC CAGACGGGTT TTCTTGTTCC 
TCCAGCCTAA GTGCTCTGAG CCTGGATGAG CCATTTATAC AGAAAGATGT AGAATTAAGA 
ATCATGCCTC CAGTTCAGGA AAACGACAAT GGGAATGAAA CTGAATCAGA ACAGCCTGAG 
GAATCAAATG AAAACCAGGA TAAAGAGGTA GAAAAGCCTG ACTCTGAAAA AGACTTATTA 
GATGATTCTG ATGACGATGA TATTGAAATA TTAGAAGAAT GTATTATTTC AGCCATGCCA 
ACAAAGTCAT CACGCAAAGC CAAAAAACTA GCCCAGACTG CTTCAAAATT ACCTCCACCT 
GTGGCAAGGA AACCAAGTCA GCTACCTGTG TATAAACTTC TGCCAGCACA GAATAGGCTG 
CAGGCACAAA AACATGTTAG CTTTACACCA GGGGATGATG TGCCCCGGGT GTACTGTGTA 
GAAGGGACAC CTATAAACTT TTCCACAGCA ACGTCTCTAA GTGATCTGAC AATAGAGTCC 
CCTCCAAATG AATTGGCTAC TGGAGATGGG GTCAGAGCGG GTATACAGTC AGGTGAATTT 
GAAAAACGAG ATACCATTCC TACAGAAGGC AGAAGTACAG ATGATGCTCA GCGAGGAAAA 
ATCTCATCTA TAGTTACACC AGACCTGGAT GACAACAAAG CAGAGGAAGG AGATATTCTT 
GCAGAATGTA TCAATTCTGC TATGCCCAAA GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA 
AAGATAATGG ACCAAGTCCA ACAAGCATCC TCGACTTCAT CTGGAGCTAA CAAAAATCAA 
GTAGACACTA AGAAAAAGAA GCCTACTTCA CCAGTAAAGC CCATGCCACA AAATACTGAA 
TATAGAACGC GTGTGAGAAA GAATACAGAC TCAAAAGTTA ATGTAAATAC TGAAGAAACT 
TTCTCAGACA ACAAAGACTC AAAGAAACCA AGCTTACAAA CCAATGCCAA GGCCTTCAAT 
GAAAAGCTAC CTAACAATGA AGACAGAGTG CGGGGGAGCT TCGCCTTGGA CTCACCGCAT 
CACTACACCC CTATTGAGGG GACGCCGTAC TGCTTTTCCC GAAATGACTC CTTGAGTTCT 
CTGGATTTTG ATGATGACGA TGTTGACCTT TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC 
AAAGAAAGCA AGGATTCCGA AGCCAAAGTT ACCTGCCGCC CAGAACCAAA CTCAAGCCAG 
CAGGCAGCTA GTAAGTCACA AGCCAGTATA AAACATCCAG CAAACAGAGC ACAGTCCAAA 
CCAGTGCTGC AGAAACAGCC CACTTTCCCC CAGTCCTCCA AAGACGGACC AGATAGAGGG 
GCAGCAACTG ACGAAAAACT GCAGAATTTT GCTATTGAAA ATACTCCAGT TTGCTTTTCT 
CGAAATTCCT CTCTGAGTTC CCTTAGTGAC ATTGACCAGG AAAACAACAA TAACAAAGAA 
AGTGAACCAA TCAAAGAAGC TGAACCTGCC AACTCACAAG GAGAGCCCAG TAAGCCTCAG 
GCATCCGGGT ATGCTCCCAA GTCCTTCCAC GTCGAAGACA CCCCTGTCTG TTTCTCAAGA 
AACAGCTCTC TCAGTTCTCT TAGCATTGAC TCTGAGGACG ACCTGTTACA GGAGTGTATA 
AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT TCAAGACTCA AGAGTGAGAG CGAAAAGCAG 
AGCCCTAGAA AAGTGGGTGG CATATTAGCT GAAGACCTGA CGCTTGATTT GAAAGATCTA 
CAGAGGCCAG ATTCAGAACA CGCTTTCTCC CCCGACTCAG AAAATTTTGA CTGGAAAGCT 
ATTCAGGAAG GCGCAAACTC CATAGTAAGT AGTTTGCACC AAGCTGCTGC AGCCGCCGCG 
TGCTTATCTA GACAAGCGTC ATCCGACTCA GATTCCATTC TGTCACTAAA GTCCGGCATT 
TCTCTGGGAT CGCCTTTTCA TCTTACACCT GATCAAGAGG AAAAGCCATT CACAAGCAAT 
AAAGGCCCAA GAATTCTCAA ACCTGGAGAG AAAAGCACAT TAGAAGCAAA AAAAATAGAA 
TCTGAAAACA AAGGAATCAA AGGCGGGAAA AAGGTTTATA AAAGCTTGAT TACGGGAAAG 
ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA ATGAAACAAC CCCTCCCGAC AAACATGCCT 
TCAATCTCAA GAGGCAGGAC GATGATTCAC ATCCCAGGGC TTCGGAATAG CTCCTCTAGT 
ACAAGCCCTG TCTCTAAGAA AGGCCCACCC CTCAAGACTC CAGCCTCTAA AAGCCCCAGT 
GAAGGGCCGG GAGCTACCAC TTCTCCTCGA GGAACTAAGC CAGCAGGAAA GTCAGAGCTT 
AGCCCTATCA CCAGGCAAAC TTCCCAAATC AGTGGGTCAA ATAAGGGGTC TTCTAGATCA 
GGATCTAGAG ACTCCACTCC CTCAAGACCT ACACAGCAAC CATTAAGTAG GCCAATGCAG 
TCTCCAGGGC GAAACTCAAT TTCCCCTGGT AGAAATGGAA TAAGCCCTCC TAACAAACTG 
TCTCAGCTGC CCAGAACATC ATCTCCCAGT ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG 
AAAATGTCAT ATACATCCCC AGGTAGACAG CTGAGCCAAC AAAATCTTAC CAAACAAGCA 
AGTTTATCCA AGAATGCCAG CAGTATCCCC AGAAGTGAGT CGGCATCTAA AGGACTGAAT 
CAGATGAGTA ACGGCAATGG GTCAAATAAA AAGGTAGAAC TTTCTAGAAT GTCTTCAACT 
AAATCAAGTG GAAGTGAATC AGACAGATCA GAAAGGCCTG CATTAGTACG CCAGTCTACT 
TTCATCAAAG AAGCCCCAAG CCCAACCCTG AGGAGGAAAC TGGAGGAATC TGCCTCATTT 
GAATCCCTTT CTCCATCTTC TAGACCAGAT TCTCCCACCA GGTCGCAGGC ACAGACCCCA 
GTTTTAAGCC CTTCCCTTCC TGATATGTCT CTGTCCACAC ATCCATCTGT TCAGGCAGGT 
GGGTGGCGAA AGCTCCCGCC TAATCTCAGC CCCACTATCG AGTATAATGA CGGAAGGCCC 
ACAAAACGGC ATGATATTGC ACGCTCCCAT TCTGAAAGTC CTTCCAGACT ACCAATCAAC 
CGGGCGGGAA CCTGGAAGCG TGAACACAGC AAACATTCCT CGTCCCTTCC TCGAGTGAGT 
ACTTGGAGAA GAACTGGAAG CTCATCTTCT ATTCTTTCTG CTTCATCAGA GTCCAGTGAA 
AAAGCAAAAA GTGAGGATGA AAGGCATGTG AGCTCCATGC CAGCACCCAG ACAGATGAAG 
GAAAACCAGG TGCCCACCAA AGGAACATGG AGGAAAATCA AGGAAAGTGA CATTTCTCCC 
ACAGGCATGG CTTCTCAGAG CGCTTCCTCA GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT 
CTGATCTATC AGATGGCACC TCCTGTCTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG 
GACTGCCCCA TTAACAACCC TAGATCTGGA CGGTCCCCCA CAGGCAACAC CCCCCCAGTG 
ATTGACAGTG TTTCAGAGAA GGGAAGTTCA AGCATTAAAG ATTCAAAAGA CACCCATGGG 
AAACAGAGTG TGGGCAGTGG CAGTCCTGTG CAAACCGTGG GTCTGGAAAC CCGCCTCAAC 
TCCTTTGTTC AGGTAGAGGC CCCAGAACAG AAAGGAACTG AGGCAAAACC AGGACAGAGT 
AACCCAGTCT CTATAGCAGA GACTGCTGAG ACGTGTATAG CAGAGCGTAC CCCTTTCAGT 
TCCAGTAGCT CCAGCAAGCA CAGCTCACCT AGCGGGACTG TTGCTGCCAG AGTGACACCT 
TTTAATTACA ACCCTAGCCC TAGGAAGAGC AGCGCAGACA GCACTTCAGC CCGGCCGTCT 
CAGATCCCTA CGCCAGTGAG CACCAACACG AAGAAGAGAG ATTCGAAGAC TGACAGCACA 
GAATCCAGTG GAGCCCAAAG TCCTAAACGC CATTCCGGGT CTTACCTCGT GACGTCTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525530.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525593.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X2, mRNA.

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CloneID	OMu54318
Clone ID Related Accession (Same CDS sequence)	NM_001360980.1 , XM_006525530.1 , XM_006525531.1 , XM_006525530.2
Accession Version	XM_006525530.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGTAGGAAG GCTACCCGGG GCATTTCATC CCAGGAGCTA 
GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC ATTGCAGAGT TATTGCAGGT GGACTGTGAG 
ATGTATGGGC TTACTAATGA CCACTACAGT GTTACTTTAA GACGGTATGC TGGAATGGCT 
TTGACAAACT TGACCTTTGG AGATGTTGCC AACAAGGCTA CGCTGTGTTC TATGAAAGGC 
TGCATGAGAG CACTTGTGGC CCAGTTAAAA TCTGAGAGTG AAGACTTACA GCAGGTTATT 
GCAAGTGTTT TGAGGAATTT GTCTTGGCGA GCAGATGTAA ATAGCAAAAA GACGTTGAGA 
GAAGTTGGAA GTGTGAAAGC ATTGATGGAA TGTGCTTTGG AAGTTAAAAA GGAATCAACC 
CTCAAAAGCG TTTTGAGTGC CTTATGGAAC CTGTCTGCAC ACTGCACTGA GAATAAGGCT 
GACATCTGTG CTGTGGATGG AGCACTGGCA TTTCTGGTTG GCACCCTCAC TTACCGGAGC 
CAGACAAATA CTTTAGCCAT TATTGAAAGT GGAGGTGGGA TATTACGGAA TGTGTCCAGC 
TTGATAGCTA CAAACGAAGA CCACAGGCAA ATCCTAAGAG AGAACAATTG CCTACAAACT 
TTATTACAGC ACTTGAAATC TCACAGCTTG ACAATAGTCA GTAATGCATG TGGAACTTTG 
TGGAATCTCT CAGCAAGAAA TCCTAAAGAC CAGGAAGCCT TGTGGGACAT GGGGGCAGTG 
AGCATGCTCA AGAACCTCAT TCATTCCAAG CACAAAATGA TTGCCATGGG AAGTGCAGCA 
GCTTTAAGGA ATCTCATGGC AAACAGACCT GCAAAGTATA AGGATGCCAA TATCATGTCT 
CCCGGCTCAA GTCTGCCATC CCTTCACGTT AGGAAACAGA AAGCTCTAGA AGCTGAGCTA 
GATGCTCAGC ATTTATCAGA AACCTTCGAC AACATTGACA ACCTAAGTCC CAAGGCCTCT 
CACCGGAGTA AGCAGAGACA CAAGCAGAAT CTTTATGGTG ACTATGCTTT TGACGCCAAT 
CGACATGATG ATAGTAGGTC AGACAATTTC AATACTGGAA ACATGACTGT TCTTTCACCA 
TATTTAAATA CTACGGTATT GCCCAGCTCT TCTTCCTCAA GGGGAAGTTT AGACAGTTCT 
CGTTCTGAGA AAGACAGAAG TTTGGAGAGA GAGCGAGGTA TTGGCCTCAG TGCTTACCAT 
CCAACAACAG AAAATGCAGG AACCTCATCA AAACGAGGTC TGCAGATCAC TACCACTGCA 
GCCCAGATAG CCAAAGTTAT GGAAGAAGTA TCAGCCATTC ATACCTCCCA GGACGACAGA 
AGTTCTGCTT CTACCACCGA GTTCCATTGT GTGGCAGACG ACAGGAGTGC GGCACGAAGA 
AGCTCTGCCT CCCACACACA CTCAAACACA TACAACTTCA CTAAGTCGGA AAATTCAAAT 
AGGACATGCT CTATGCCTTA TGCCAAAGTG GAATATAAAC GATCTTCAAA TGACAGTTTA 
AATAGTGTCA CTAGTAGTGA TGGATATGGT AAAAGAGGCC AAATGAAACC CTCAGTTGAA 
TCCTATTCTG AAGATGATGA AAGTAAATTT TGCAGTTATG GTCAGTATCC AGCTGACCTA 
GCCCATAAGA TACACAGTGC AAATCATATG GATGATAATG ATGGAGAACT GGATACACCA 
ATAAATTACA GTCTTAAATA TTCAGATGAG CAGTTGAACT CAGGAAGGCA GAGTCCCTCA 
CAGAATGAAA GGTGGGCAAG ACCAAAGCAT GTGATAGAAG ATGAAATAAA GCAAAACGAG 
CAAAGACAAG CAAGAAGCCA GAACACCAGT TATCCTGTCT ATTCTGAGAA TACCGATGAC 
AAACACCTCA AATTCCAACC ACATTTTGGA CAACAAGAAT GTGTTTCCCC ATATAGGTCA 
AGGGGAACCA GTGGTTCAGA AACAAATCGA ATGGGTTCTA GTCATGCAAT TAATCAAAAT 
GTAAACCAGT CTCTGTGTCA GGAAGATGAT TATGAAGATG ATAAACCTAC CAACTACAGT 
GAACGTTATT CTGAGGAAGA ACAACATGAA GAAGAAGAAG AGAGACCGAC AAATTATAGC 
ATAAAATATA ATGAAGAGAA ACATCATGTG GATCAGCCTA TTGATTATAG TTTAAAATAT 
GCCACTGACA TTTCTTCCTC ACAAAAACCA TCATTTTCAT TCTCAAAGAA TTCATCAGCA 
CAAAGCACTA AACCTGAACA TCTCTCTCCA AGCAGCGAGA ATACAGCTGT ACCTCCATCT 
AATGCCAAAA GGCAGAATCA GCTGCGTCCA AGTTCAGCAC AAAGAAATGG CCAGACTCAA 
AAAGGCACTA CTTGCAAAGT CCCCTCCATC AACCAAGAAA CAATACAGAC TTACTGCGTA 
GAAGACACCC CAATATGTTT TTCAAGGTGC AGTTCATTAT CATCACTGTC ATCAGCTGAC 
GATGAAATAG GATGTGATCA GACAACACAG GAAGCAGATT CTGCTAATAC TCTGCAGACA 
GCAGAAGTAA AAGAGAATGA TGTAACTCGG TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA 
GCAGTGTCCC AGAATGCTAG AGCCAAACCC AGCCGACTCC AGGCTTCTGG CTTATCTTCA 
GAATCAACCA GGCATAATAA AGCTGTTGAG TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA 
AGTGGTGCTC AGACACCCAA AAGTCCCCCA GAACACTATG TCCAGGAGAC TCCGCTCGTA 
TTCAGCAGGT GTACTTCTGT CAGCTCCCTT GACAGTTTTG AGAGTCGCTC CATTGCCAGC 
TCTGTTCAGA GTGAGCCATG TAGTGGAATG GTGAGTGGCA TCATAAGCCC CAGTGACCTT 
CCAGATAGTC CTGGGCAGAC CATGCCACCA AGCAGAAGCA AAACCCCTCC ACCTCCTCCA 
CAGACAGTGC AGGCCAAGAG AGAGGTGCCA AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA 
GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA 
GACGTGGATA CTTTGTTACA CTTCGCCACA GAAAGTACTC CAGACGGGTT TTCTTGTTCC 
TCCAGCCTAA GTGCTCTGAG CCTGGATGAG CCATTTATAC AGAAAGATGT AGAATTAAGA 
ATCATGCCTC CAGTTCAGGA AAACGACAAT GGGAATGAAA CTGAATCAGA ACAGCCTGAG 
GAATCAAATG AAAACCAGGA TAAAGAGGTA GAAAAGCCTG ACTCTGAAAA AGACTTATTA 
GATGATTCTG ATGACGATGA TATTGAAATA TTAGAAGAAT GTATTATTTC AGCCATGCCA 
ACAAAGTCAT CACGCAAAGC CAAAAAACTA GCCCAGACTG CTTCAAAATT ACCTCCACCT 
GTGGCAAGGA AACCAAGTCA GCTACCTGTG TATAAACTTC TGCCAGCACA GAATAGGCTG 
CAGGCACAAA AACATGTTAG CTTTACACCA GGGGATGATG TGCCCCGGGT GTACTGTGTA 
GAAGGGACAC CTATAAACTT TTCCACAGCA ACGTCTCTAA GTGATCTGAC AATAGAGTCC 
CCTCCAAATG AATTGGCTAC TGGAGATGGG GTCAGAGCGG GTATACAGTC AGGTGAATTT 
GAAAAACGAG ATACCATTCC TACAGAAGGC AGAAGTACAG ATGATGCTCA GCGAGGAAAA 
ATCTCATCTA TAGTTACACC AGACCTGGAT GACAACAAAG CAGAGGAAGG AGATATTCTT 
GCAGAATGTA TCAATTCTGC TATGCCCAAA GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA 
AAGATAATGG ACCAAGTCCA ACAAGCATCC TCGACTTCAT CTGGAGCTAA CAAAAATCAA 
GTAGACACTA AGAAAAAGAA GCCTACTTCA CCAGTAAAGC CCATGCCACA AAATACTGAA 
TATAGAACGC GTGTGAGAAA GAATACAGAC TCAAAAGTTA ATGTAAATAC TGAAGAAACT 
TTCTCAGACA ACAAAGACTC AAAGAAACCA AGCTTACAAA CCAATGCCAA GGCCTTCAAT 
GAAAAGCTAC CTAACAATGA AGACAGAGTG CGGGGGAGCT TCGCCTTGGA CTCACCGCAT 
CACTACACCC CTATTGAGGG GACGCCGTAC TGCTTTTCCC GAAATGACTC CTTGAGTTCT 
CTGGATTTTG ATGATGACGA TGTTGACCTT TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC 
AAAGAAAGCA AGGATTCCGA AGCCAAAGTT ACCTGCCGCC CAGAACCAAA CTCAAGCCAG 
CAGGCAGCTA GTAAGTCACA AGCCAGTATA AAACATCCAG CAAACAGAGC ACAGTCCAAA 
CCAGTGCTGC AGAAACAGCC CACTTTCCCC CAGTCCTCCA AAGACGGACC AGATAGAGGG 
GCAGCAACTG ACGAAAAACT GCAGAATTTT GCTATTGAAA ATACTCCAGT TTGCTTTTCT 
CGAAATTCCT CTCTGAGTTC CCTTAGTGAC ATTGACCAGG AAAACAACAA TAACAAAGAA 
AGTGAACCAA TCAAAGAAGC TGAACCTGCC AACTCACAAG GAGAGCCCAG TAAGCCTCAG 
GCATCCGGGT ATGCTCCCAA GTCCTTCCAC GTCGAAGACA CCCCTGTCTG TTTCTCAAGA 
AACAGCTCTC TCAGTTCTCT TAGCATTGAC TCTGAGGACG ACCTGTTACA GGAGTGTATA 
AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT TCAAGACTCA AGAGTGAGAG CGAAAAGCAG 
AGCCCTAGAA AAGTGGGTGG CATATTAGCT GAAGACCTGA CGCTTGATTT GAAAGATCTA 
CAGAGGCCAG ATTCAGAACA CGCTTTCTCC CCCGACTCAG AAAATTTTGA CTGGAAAGCT 
ATTCAGGAAG GCGCAAACTC CATAGTAAGT AGTTTGCACC AAGCTGCTGC AGCCGCCGCG 
TGCTTATCTA GACAAGCGTC ATCCGACTCA GATTCCATTC TGTCACTAAA GTCCGGCATT 
TCTCTGGGAT CGCCTTTTCA TCTTACACCT GATCAAGAGG AAAAGCCATT CACAAGCAAT 
AAAGGCCCAA GAATTCTCAA ACCTGGAGAG AAAAGCACAT TAGAAGCAAA AAAAATAGAA 
TCTGAAAACA AAGGAATCAA AGGCGGGAAA AAGGTTTATA AAAGCTTGAT TACGGGAAAG 
ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA ATGAAACAAC CCCTCCCGAC AAACATGCCT 
TCAATCTCAA GAGGCAGGAC GATGATTCAC ATCCCAGGGC TTCGGAATAG CTCCTCTAGT 
ACAAGCCCTG TCTCTAAGAA AGGCCCACCC CTCAAGACTC CAGCCTCTAA AAGCCCCAGT 
GAAGGGCCGG GAGCTACCAC TTCTCCTCGA GGAACTAAGC CAGCAGGAAA GTCAGAGCTT 
AGCCCTATCA CCAGGCAAAC TTCCCAAATC AGTGGGTCAA ATAAGGGGTC TTCTAGATCA 
GGATCTAGAG ACTCCACTCC CTCAAGACCT ACACAGCAAC CATTAAGTAG GCCAATGCAG 
TCTCCAGGGC GAAACTCAAT TTCCCCTGGT AGAAATGGAA TAAGCCCTCC TAACAAACTG 
TCTCAGCTGC CCAGAACATC ATCTCCCAGT ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG 
AAAATGTCAT ATACATCCCC AGGTAGACAG CTGAGCCAAC AAAATCTTAC CAAACAAGCA 
AGTTTATCCA AGAATGCCAG CAGTATCCCC AGAAGTGAGT CGGCATCTAA AGGACTGAAT 
CAGATGAGTA ACGGCAATGG GTCAAATAAA AAGGTAGAAC TTTCTAGAAT GTCTTCAACT 
AAATCAAGTG GAAGTGAATC AGACAGATCA GAAAGGCCTG CATTAGTACG CCAGTCTACT 
TTCATCAAAG AAGCCCCAAG CCCAACCCTG AGGAGGAAAC TGGAGGAATC TGCCTCATTT 
GAATCCCTTT CTCCATCTTC TAGACCAGAT TCTCCCACCA GGTCGCAGGC ACAGACCCCA 
GTTTTAAGCC CTTCCCTTCC TGATATGTCT CTGTCCACAC ATCCATCTGT TCAGGCAGGT 
GGGTGGCGAA AGCTCCCGCC TAATCTCAGC CCCACTATCG AGTATAATGA CGGAAGGCCC 
ACAAAACGGC ATGATATTGC ACGCTCCCAT TCTGAAAGTC CTTCCAGACT ACCAATCAAC 
CGGGCGGGAA CCTGGAAGCG TGAACACAGC AAACATTCCT CGTCCCTTCC TCGAGTGAGT 
ACTTGGAGAA GAACTGGAAG CTCATCTTCT ATTCTTTCTG CTTCATCAGA GTCCAGTGAA 
AAAGCAAAAA GTGAGGATGA AAGGCATGTG AGCTCCATGC CAGCACCCAG ACAGATGAAG 
GAAAACCAGG TGCCCACCAA AGGAACATGG AGGAAAATCA AGGAAAGTGA CATTTCTCCC 
ACAGGCATGG CTTCTCAGAG CGCTTCCTCA GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT 
CTGATCTATC AGATGGCACC TCCTGTCTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG 
GACTGCCCCA TTAACAACCC TAGATCTGGA CGGTCCCCCA CAGGCAACAC CCCCCCAGTG 
ATTGACAGTG TTTCAGAGAA GGGAAGTTCA AGCATTAAAG ATTCAAAAGA CACCCATGGG 
AAACAGAGTG TGGGCAGTGG CAGTCCTGTG CAAACCGTGG GTCTGGAAAC CCGCCTCAAC 
TCCTTTGTTC AGGTAGAGGC CCCAGAACAG AAAGGAACTG AGGCAAAACC AGGACAGAGT 
AACCCAGTCT CTATAGCAGA GACTGCTGAG ACGTGTATAG CAGAGCGTAC CCCTTTCAGT 
TCCAGTAGCT CCAGCAAGCA CAGCTCACCT AGCGGGACTG TTGCTGCCAG AGTGACACCT 
TTTAATTACA ACCCTAGCCC TAGGAAGAGC AGCGCAGACA GCACTTCAGC CCGGCCGTCT 
CAGATCCCTA CGCCAGTGAG CACCAACACG AAGAAGAGAG ATTCGAAGAC TGACAGCACA 
GAATCCAGTG GAGCCCAAAG TCCTAAACGC CATTCCGGGT CTTACCTCGT GACGTCTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525593.1
CDS	413..8995
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525530.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525530.2

ORF Insert Sequence:

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGTAGGAAG GCTACCCGGG GCATTTCATC CCAGGAGCTA 
GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC ATTGCAGAGT TATTGCAGGT GGACTGTGAG 
ATGTATGGGC TTACTAATGA CCACTACAGT GTTACTTTAA GACGGTATGC TGGAATGGCT 
TTGACAAACT TGACCTTTGG AGATGTTGCC AACAAGGCTA CGCTGTGTTC TATGAAAGGC 
TGCATGAGAG CACTTGTGGC CCAGTTAAAA TCTGAGAGTG AAGACTTACA GCAGGTTATT 
GCAAGTGTTT TGAGGAATTT GTCTTGGCGA GCAGATGTAA ATAGCAAAAA GACGTTGAGA 
GAAGTTGGAA GTGTGAAAGC ATTGATGGAA TGTGCTTTGG AAGTTAAAAA GGAATCAACC 
CTCAAAAGCG TTTTGAGTGC CTTATGGAAC CTGTCTGCAC ACTGCACTGA GAATAAGGCT 
GACATCTGTG CTGTGGATGG AGCACTGGCA TTTCTGGTTG GCACCCTCAC TTACCGGAGC 
CAGACAAATA CTTTAGCCAT TATTGAAAGT GGAGGTGGGA TATTACGGAA TGTGTCCAGC 
TTGATAGCTA CAAACGAAGA CCACAGGCAA ATCCTAAGAG AGAACAATTG CCTACAAACT 
TTATTACAGC ACTTGAAATC TCACAGCTTG ACAATAGTCA GTAATGCATG TGGAACTTTG 
TGGAATCTCT CAGCAAGAAA TCCTAAAGAC CAGGAAGCCT TGTGGGACAT GGGGGCAGTG 
AGCATGCTCA AGAACCTCAT TCATTCCAAG CACAAAATGA TTGCCATGGG AAGTGCAGCA 
GCTTTAAGGA ATCTCATGGC AAACAGACCT GCAAAGTATA AGGATGCCAA TATCATGTCT 
CCCGGCTCAA GTCTGCCATC CCTTCACGTT AGGAAACAGA AAGCTCTAGA AGCTGAGCTA 
GATGCTCAGC ATTTATCAGA AACCTTCGAC AACATTGACA ACCTAAGTCC CAAGGCCTCT 
CACCGGAGTA AGCAGAGACA CAAGCAGAAT CTTTATGGTG ACTATGCTTT TGACGCCAAT 
CGACATGATG ATAGTAGGTC AGACAATTTC AATACTGGAA ACATGACTGT TCTTTCACCA 
TATTTAAATA CTACGGTATT GCCCAGCTCT TCTTCCTCAA GGGGAAGTTT AGACAGTTCT 
CGTTCTGAGA AAGACAGAAG TTTGGAGAGA GAGCGAGGTA TTGGCCTCAG TGCTTACCAT 
CCAACAACAG AAAATGCAGG AACCTCATCA AAACGAGGTC TGCAGATCAC TACCACTGCA 
GCCCAGATAG CCAAAGTTAT GGAAGAAGTA TCAGCCATTC ATACCTCCCA GGACGACAGA 
AGTTCTGCTT CTACCACCGA GTTCCATTGT GTGGCAGACG ACAGGAGTGC GGCACGAAGA 
AGCTCTGCCT CCCACACACA CTCAAACACA TACAACTTCA CTAAGTCGGA AAATTCAAAT 
AGGACATGCT CTATGCCTTA TGCCAAAGTG GAATATAAAC GATCTTCAAA TGACAGTTTA 
AATAGTGTCA CTAGTAGTGA TGGATATGGT AAAAGAGGCC AAATGAAACC CTCAGTTGAA 
TCCTATTCTG AAGATGATGA AAGTAAATTT TGCAGTTATG GTCAGTATCC AGCTGACCTA 
GCCCATAAGA TACACAGTGC AAATCATATG GATGATAATG ATGGAGAACT GGATACACCA 
ATAAATTACA GTCTTAAATA TTCAGATGAG CAGTTGAACT CAGGAAGGCA GAGTCCCTCA 
CAGAATGAAA GGTGGGCAAG ACCAAAGCAT GTGATAGAAG ATGAAATAAA GCAAAACGAG 
CAAAGACAAG CAAGAAGCCA GAACACCAGT TATCCTGTCT ATTCTGAGAA TACCGATGAC 
AAACACCTCA AATTCCAACC ACATTTTGGA CAACAAGAAT GTGTTTCCCC ATATAGGTCA 
AGGGGAACCA GTGGTTCAGA AACAAATCGA ATGGGTTCTA GTCATGCAAT TAATCAAAAT 
GTAAACCAGT CTCTGTGTCA GGAAGATGAT TATGAAGATG ATAAACCTAC CAACTACAGT 
GAACGTTATT CTGAGGAAGA ACAACATGAA GAAGAAGAAG AGAGACCGAC AAATTATAGC 
ATAAAATATA ATGAAGAGAA ACATCATGTG GATCAGCCTA TTGATTATAG TTTAAAATAT 
GCCACTGACA TTTCTTCCTC ACAAAAACCA TCATTTTCAT TCTCAAAGAA TTCATCAGCA 
CAAAGCACTA AACCTGAACA TCTCTCTCCA AGCAGCGAGA ATACAGCTGT ACCTCCATCT 
AATGCCAAAA GGCAGAATCA GCTGCGTCCA AGTTCAGCAC AAAGAAATGG CCAGACTCAA 
AAAGGCACTA CTTGCAAAGT CCCCTCCATC AACCAAGAAA CAATACAGAC TTACTGCGTA 
GAAGACACCC CAATATGTTT TTCAAGGTGC AGTTCATTAT CATCACTGTC ATCAGCTGAC 
GATGAAATAG GATGTGATCA GACAACACAG GAAGCAGATT CTGCTAATAC TCTGCAGACA 
GCAGAAGTAA AAGAGAATGA TGTAACTCGG TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA 
GCAGTGTCCC AGAATGCTAG AGCCAAACCC AGCCGACTCC AGGCTTCTGG CTTATCTTCA 
GAATCAACCA GGCATAATAA AGCTGTTGAG TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA 
AGTGGTGCTC AGACACCCAA AAGTCCCCCA GAACACTATG TCCAGGAGAC TCCGCTCGTA 
TTCAGCAGGT GTACTTCTGT CAGCTCCCTT GACAGTTTTG AGAGTCGCTC CATTGCCAGC 
TCTGTTCAGA GTGAGCCATG TAGTGGAATG GTGAGTGGCA TCATAAGCCC CAGTGACCTT 
CCAGATAGTC CTGGGCAGAC CATGCCACCA AGCAGAAGCA AAACCCCTCC ACCTCCTCCA 
CAGACAGTGC AGGCCAAGAG AGAGGTGCCA AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA 
GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA 
GACGTGGATA CTTTGTTACA CTTCGCCACA GAAAGTACTC CAGACGGGTT TTCTTGTTCC 
TCCAGCCTAA GTGCTCTGAG CCTGGATGAG CCATTTATAC AGAAAGATGT AGAATTAAGA 
ATCATGCCTC CAGTTCAGGA AAACGACAAT GGGAATGAAA CTGAATCAGA ACAGCCTGAG 
GAATCAAATG AAAACCAGGA TAAAGAGGTA GAAAAGCCTG ACTCTGAAAA AGACTTATTA 
GATGATTCTG ATGACGATGA TATTGAAATA TTAGAAGAAT GTATTATTTC AGCCATGCCA 
ACAAAGTCAT CACGCAAAGC CAAAAAACTA GCCCAGACTG CTTCAAAATT ACCTCCACCT 
GTGGCAAGGA AACCAAGTCA GCTACCTGTG TATAAACTTC TGCCAGCACA GAATAGGCTG 
CAGGCACAAA AACATGTTAG CTTTACACCA GGGGATGATG TGCCCCGGGT GTACTGTGTA 
GAAGGGACAC CTATAAACTT TTCCACAGCA ACGTCTCTAA GTGATCTGAC AATAGAGTCC 
CCTCCAAATG AATTGGCTAC TGGAGATGGG GTCAGAGCGG GTATACAGTC AGGTGAATTT 
GAAAAACGAG ATACCATTCC TACAGAAGGC AGAAGTACAG ATGATGCTCA GCGAGGAAAA 
ATCTCATCTA TAGTTACACC AGACCTGGAT GACAACAAAG CAGAGGAAGG AGATATTCTT 
GCAGAATGTA TCAATTCTGC TATGCCCAAA GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA 
AAGATAATGG ACCAAGTCCA ACAAGCATCC TCGACTTCAT CTGGAGCTAA CAAAAATCAA 
GTAGACACTA AGAAAAAGAA GCCTACTTCA CCAGTAAAGC CCATGCCACA AAATACTGAA 
TATAGAACGC GTGTGAGAAA GAATACAGAC TCAAAAGTTA ATGTAAATAC TGAAGAAACT 
TTCTCAGACA ACAAAGACTC AAAGAAACCA AGCTTACAAA CCAATGCCAA GGCCTTCAAT 
GAAAAGCTAC CTAACAATGA AGACAGAGTG CGGGGGAGCT TCGCCTTGGA CTCACCGCAT 
CACTACACCC CTATTGAGGG GACGCCGTAC TGCTTTTCCC GAAATGACTC CTTGAGTTCT 
CTGGATTTTG ATGATGACGA TGTTGACCTT TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC 
AAAGAAAGCA AGGATTCCGA AGCCAAAGTT ACCTGCCGCC CAGAACCAAA CTCAAGCCAG 
CAGGCAGCTA GTAAGTCACA AGCCAGTATA AAACATCCAG CAAACAGAGC ACAGTCCAAA 
CCAGTGCTGC AGAAACAGCC CACTTTCCCC CAGTCCTCCA AAGACGGACC AGATAGAGGG 
GCAGCAACTG ACGAAAAACT GCAGAATTTT GCTATTGAAA ATACTCCAGT TTGCTTTTCT 
CGAAATTCCT CTCTGAGTTC CCTTAGTGAC ATTGACCAGG AAAACAACAA TAACAAAGAA 
AGTGAACCAA TCAAAGAAGC TGAACCTGCC AACTCACAAG GAGAGCCCAG TAAGCCTCAG 
GCATCCGGGT ATGCTCCCAA GTCCTTCCAC GTCGAAGACA CCCCTGTCTG TTTCTCAAGA 
AACAGCTCTC TCAGTTCTCT TAGCATTGAC TCTGAGGACG ACCTGTTACA GGAGTGTATA 
AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT TCAAGACTCA AGAGTGAGAG CGAAAAGCAG 
AGCCCTAGAA AAGTGGGTGG CATATTAGCT GAAGACCTGA CGCTTGATTT GAAAGATCTA 
CAGAGGCCAG ATTCAGAACA CGCTTTCTCC CCCGACTCAG AAAATTTTGA CTGGAAAGCT 
ATTCAGGAAG GCGCAAACTC CATAGTAAGT AGTTTGCACC AAGCTGCTGC AGCCGCCGCG 
TGCTTATCTA GACAAGCGTC ATCCGACTCA GATTCCATTC TGTCACTAAA GTCCGGCATT 
TCTCTGGGAT CGCCTTTTCA TCTTACACCT GATCAAGAGG AAAAGCCATT CACAAGCAAT 
AAAGGCCCAA GAATTCTCAA ACCTGGAGAG AAAAGCACAT TAGAAGCAAA AAAAATAGAA 
TCTGAAAACA AAGGAATCAA AGGCGGGAAA AAGGTTTATA AAAGCTTGAT TACGGGAAAG 
ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA ATGAAACAAC CCCTCCCGAC AAACATGCCT 
TCAATCTCAA GAGGCAGGAC GATGATTCAC ATCCCAGGGC TTCGGAATAG CTCCTCTAGT 
ACAAGCCCTG TCTCTAAGAA AGGCCCACCC CTCAAGACTC CAGCCTCTAA AAGCCCCAGT 
GAAGGGCCGG GAGCTACCAC TTCTCCTCGA GGAACTAAGC CAGCAGGAAA GTCAGAGCTT 
AGCCCTATCA CCAGGCAAAC TTCCCAAATC AGTGGGTCAA ATAAGGGGTC TTCTAGATCA 
GGATCTAGAG ACTCCACTCC CTCAAGACCT ACACAGCAAC CATTAAGTAG GCCAATGCAG 
TCTCCAGGGC GAAACTCAAT TTCCCCTGGT AGAAATGGAA TAAGCCCTCC TAACAAACTG 
TCTCAGCTGC CCAGAACATC ATCTCCCAGT ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG 
AAAATGTCAT ATACATCCCC AGGTAGACAG CTGAGCCAAC AAAATCTTAC CAAACAAGCA 
AGTTTATCCA AGAATGCCAG CAGTATCCCC AGAAGTGAGT CGGCATCTAA AGGACTGAAT 
CAGATGAGTA ACGGCAATGG GTCAAATAAA AAGGTAGAAC TTTCTAGAAT GTCTTCAACT 
AAATCAAGTG GAAGTGAATC AGACAGATCA GAAAGGCCTG CATTAGTACG CCAGTCTACT 
TTCATCAAAG AAGCCCCAAG CCCAACCCTG AGGAGGAAAC TGGAGGAATC TGCCTCATTT 
GAATCCCTTT CTCCATCTTC TAGACCAGAT TCTCCCACCA GGTCGCAGGC ACAGACCCCA 
GTTTTAAGCC CTTCCCTTCC TGATATGTCT CTGTCCACAC ATCCATCTGT TCAGGCAGGT 
GGGTGGCGAA AGCTCCCGCC TAATCTCAGC CCCACTATCG AGTATAATGA CGGAAGGCCC 
ACAAAACGGC ATGATATTGC ACGCTCCCAT TCTGAAAGTC CTTCCAGACT ACCAATCAAC 
CGGGCGGGAA CCTGGAAGCG TGAACACAGC AAACATTCCT CGTCCCTTCC TCGAGTGAGT 
ACTTGGAGAA GAACTGGAAG CTCATCTTCT ATTCTTTCTG CTTCATCAGA GTCCAGTGAA 
AAAGCAAAAA GTGAGGATGA AAGGCATGTG AGCTCCATGC CAGCACCCAG ACAGATGAAG 
GAAAACCAGG TGCCCACCAA AGGAACATGG AGGAAAATCA AGGAAAGTGA CATTTCTCCC 
ACAGGCATGG CTTCTCAGAG CGCTTCCTCA GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT 
CTGATCTATC AGATGGCACC TCCTGTCTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG 
GACTGCCCCA TTAACAACCC TAGATCTGGA CGGTCCCCCA CAGGCAACAC CCCCCCAGTG 
ATTGACAGTG TTTCAGAGAA GGGAAGTTCA AGCATTAAAG ATTCAAAAGA CACCCATGGG 
AAACAGAGTG TGGGCAGTGG CAGTCCTGTG CAAACCGTGG GTCTGGAAAC CCGCCTCAAC 
TCCTTTGTTC AGGTAGAGGC CCCAGAACAG AAAGGAACTG AGGCAAAACC AGGACAGAGT 
AACCCAGTCT CTATAGCAGA GACTGCTGAG ACGTGTATAG CAGAGCGTAC CCCTTTCAGT 
TCCAGTAGCT CCAGCAAGCA CAGCTCACCT AGCGGGACTG TTGCTGCCAG AGTGACACCT 
TTTAATTACA ACCCTAGCCC TAGGAAGAGC AGCGCAGACA GCACTTCAGC CCGGCCGTCT 
CAGATCCCTA CGCCAGTGAG CACCAACACG AAGAAGAGAG ATTCGAAGAC TGACAGCACA 
GAATCCAGTG GAGCCCAAAG TCCTAAACGC CATTCCGGGT CTTACCTCGT GACGTCTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525532.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525595.1 Mus musculus adenomatosis polyposis coli (Apc), transcript variant X4, mRNA.

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CloneID	OMu54319
Clone ID Related Accession (Same CDS sequence)	XM_006525532.3 , XM_006525532.4
Accession Version	XM_006525532.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525532.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGGTG 
GAAATGGTGT ATTCCTTGTT GTCAATGCTT GGTACTCATG ATAAGGACGA TATGTCACGA 
ACTTTGCTAG CTATGTCCAG CTCCCAAGAC AGCTGTATAT CCATGCGGCA GTCTGGATGT 
CTTCCTCTCC TCATCCAGCT TTTACATGGC AATGACAAAG ACTCTGTATT GTTGGGAAAT 
TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG GCCAGTGCAG CACTCCACAA CATCATTCAC 
TCACAGCCTG ATGACAAGAG AGGCAGGCGT GAAATCCGAG TCCTTCATCT TTTGGAACAG 
ATACGAGCTT ACTGTGAAAC CTGTTGGGAG TGGCAGGAAG CCCACGAACA AGGCATGGAC 
CAGGACAAAA ACCCAATGCC AGCTCCTGTT GAGCATCAGA TCTGTCCTGC TGTGTGTGTT 
CTAATGAAGC TTTCATTTGA TGAAGAGCAT AGGCATGCAA TGAATGAACT TGGGGGACTG 
CAGGCCATTG CAGAGTTATT GCAGGTGGAC TGTGAGATGT ATGGGCTTAC TAATGACCAC 
TACAGTGTTA CTTTAAGACG GTATGCTGGA ATGGCTTTGA CAAACTTGAC CTTTGGAGAT 
GTTGCCAACA AGGCTACGCT GTGTTCTATG AAAGGCTGCA TGAGAGCACT TGTGGCCCAG 
TTAAAATCTG AGAGTGAAGA CTTACAGCAG GTTATTGCAA GTGTTTTGAG GAATTTGTCT 
TGGCGAGCAG ATGTAAATAG CAAAAAGACG TTGAGAGAAG TTGGAAGTGT GAAAGCATTG 
ATGGAATGTG CTTTGGAAGT TAAAAAGGAA TCAACCCTCA AAAGCGTTTT GAGTGCCTTA 
TGGAACCTGT CTGCACACTG CACTGAGAAT AAGGCTGACA TCTGTGCTGT GGATGGAGCA 
CTGGCATTTC TGGTTGGCAC CCTCACTTAC CGGAGCCAGA CAAATACTTT AGCCATTATT 
GAAAGTGGAG GTGGGATATT ACGGAATGTG TCCAGCTTGA TAGCTACAAA CGAAGACCAC 
AGGCAAATCC TAAGAGAGAA CAATTGCCTA CAAACTTTAT TACAGCACTT GAAATCTCAC 
AGCTTGACAA TAGTCAGTAA TGCATGTGGA ACTTTGTGGA ATCTCTCAGC AAGAAATCCT 
AAAGACCAGG AAGCCTTGTG GGACATGGGG GCAGTGAGCA TGCTCAAGAA CCTCATTCAT 
TCCAAGCACA AAATGATTGC CATGGGAAGT GCAGCAGCTT TAAGGAATCT CATGGCAAAC 
AGACCTGCAA AGTATAAGGA TGCCAATATC ATGTCTCCCG GCTCAAGTCT GCCATCCCTT 
CACGTTAGGA AACAGAAAGC TCTAGAAGCT GAGCTAGATG CTCAGCATTT ATCAGAAACC 
TTCGACAACA TTGACAACCT AAGTCCCAAG GCCTCTCACC GGAGTAAGCA GAGACACAAG 
CAGAATCTTT ATGGTGACTA TGCTTTTGAC GCCAATCGAC ATGATGATAG TAGGTCAGAC 
AATTTCAATA CTGGAAACAT GACTGTTCTT TCACCATATT TAAATACTAC GGTATTGCCC 
AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG 
GAGAGAGAGC GAGGTATTGG CCTCAGTGCT TACCATCCAA CAACAGAAAA TGCAGGAACC 
TCATCAAAAC GAGGTCTGCA GATCACTACC ACTGCAGCCC AGATAGCCAA AGTTATGGAA 
GAAGTATCAG CCATTCATAC CTCCCAGGAC GACAGAAGTT CTGCTTCTAC CACCGAGTTC 
CATTGTGTGG CAGACGACAG GAGTGCGGCA CGAAGAAGCT CTGCCTCCCA CACACACTCA 
AACACATACA ACTTCACTAA GTCGGAAAAT TCAAATAGGA CATGCTCTAT GCCTTATGCC 
AAAGTGGAAT ATAAACGATC TTCAAATGAC AGTTTAAATA GTGTCACTAG TAGTGATGGA 
TATGGTAAAA GAGGCCAAAT GAAACCCTCA GTTGAATCCT ATTCTGAAGA TGATGAAAGT 
AAATTTTGCA GTTATGGTCA GTATCCAGCT GACCTAGCCC ATAAGATACA CAGTGCAAAT 
CATATGGATG ATAATGATGG AGAACTGGAT ACACCAATAA ATTACAGTCT TAAATATTCA 
GATGAGCAGT TGAACTCAGG AAGGCAGAGT CCCTCACAGA ATGAAAGGTG GGCAAGACCA 
AAGCATGTGA TAGAAGATGA AATAAAGCAA AACGAGCAAA GACAAGCAAG AAGCCAGAAC 
ACCAGTTATC CTGTCTATTC TGAGAATACC GATGACAAAC ACCTCAAATT CCAACCACAT 
TTTGGACAAC AAGAATGTGT TTCCCCATAT AGGTCAAGGG GAACCAGTGG TTCAGAAACA 
AATCGAATGG GTTCTAGTCA TGCAATTAAT CAAAATGTAA ACCAGTCTCT GTGTCAGGAA 
GATGATTATG AAGATGATAA ACCTACCAAC TACAGTGAAC GTTATTCTGA GGAAGAACAA 
CATGAAGAAG AAGAAGAGAG ACCGACAAAT TATAGCATAA AATATAATGA AGAGAAACAT 
CATGTGGATC AGCCTATTGA TTATAGTTTA AAATATGCCA CTGACATTTC TTCCTCACAA 
AAACCATCAT TTTCATTCTC AAAGAATTCA TCAGCACAAA GCACTAAACC TGAACATCTC 
TCTCCAAGCA GCGAGAATAC AGCTGTACCT CCATCTAATG CCAAAAGGCA GAATCAGCTG 
CGTCCAAGTT CAGCACAAAG AAATGGCCAG ACTCAAAAAG GCACTACTTG CAAAGTCCCC 
TCCATCAACC AAGAAACAAT ACAGACTTAC TGCGTAGAAG ACACCCCAAT ATGTTTTTCA 
AGGTGCAGTT CATTATCATC ACTGTCATCA GCTGACGATG AAATAGGATG TGATCAGACA 
ACACAGGAAG CAGATTCTGC TAATACTCTG CAGACAGCAG AAGTAAAAGA GAATGATGTA 
ACTCGGTCAG CTGAAGATCC TGCAACTGAA GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC 
AAACCCAGCC GACTCCAGGC TTCTGGCTTA TCTTCAGAAT CAACCAGGCA TAATAAAGCT 
GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT 
CCCCCAGAAC ACTATGTCCA GGAGACTCCG CTCGTATTCA GCAGGTGTAC TTCTGTCAGC 
TCCCTTGACA GTTTTGAGAG TCGCTCCATT GCCAGCTCTG TTCAGAGTGA GCCATGTAGT 
GGAATGGTGA GTGGCATCAT AAGCCCCAGT GACCTTCCAG ATAGTCCTGG GCAGACCATG 
CCACCAAGCA GAAGCAAAAC CCCTCCACCT CCTCCACAGA CAGTGCAGGC CAAGAGAGAG 
GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG AAGAGAGAGA GTGGGCCTAA GCAGACTGCT 
GTAAATGCTG CCGTGCAGAG GGTGCAGGTC CTTCCAGACG TGGATACTTT GTTACACTTC 
GCCACAGAAA GTACTCCAGA CGGGTTTTCT TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG 
GATGAGCCAT TTATACAGAA AGATGTAGAA TTAAGAATCA TGCCTCCAGT TCAGGAAAAC 
GACAATGGGA ATGAAACTGA ATCAGAACAG CCTGAGGAAT CAAATGAAAA CCAGGATAAA 
GAGGTAGAAA AGCCTGACTC TGAAAAAGAC TTATTAGATG ATTCTGATGA CGATGATATT 
GAAATATTAG AAGAATGTAT TATTTCAGCC ATGCCAACAA AGTCATCACG CAAAGCCAAA 
AAACTAGCCC AGACTGCTTC AAAATTACCT CCACCTGTGG CAAGGAAACC AAGTCAGCTA 
CCTGTGTATA AACTTCTGCC AGCACAGAAT AGGCTGCAGG CACAAAAACA TGTTAGCTTT 
ACACCAGGGG ATGATGTGCC CCGGGTGTAC TGTGTAGAAG GGACACCTAT AAACTTTTCC 
ACAGCAACGT CTCTAAGTGA TCTGACAATA GAGTCCCCTC CAAATGAATT GGCTACTGGA 
GATGGGGTCA GAGCGGGTAT ACAGTCAGGT GAATTTGAAA AACGAGATAC CATTCCTACA 
GAAGGCAGAA GTACAGATGA TGCTCAGCGA GGAAAAATCT CATCTATAGT TACACCAGAC 
CTGGATGACA ACAAAGCAGA GGAAGGAGAT ATTCTTGCAG AATGTATCAA TTCTGCTATG 
CCCAAAGGAA AAAGCCACAA GCCTTTCCGA GTGAAAAAGA TAATGGACCA AGTCCAACAA 
GCATCCTCGA CTTCATCTGG AGCTAACAAA AATCAAGTAG ACACTAAGAA AAAGAAGCCT 
ACTTCACCAG TAAAGCCCAT GCCACAAAAT ACTGAATATA GAACGCGTGT GAGAAAGAAT 
ACAGACTCAA AAGTTAATGT AAATACTGAA GAAACTTTCT CAGACAACAA AGACTCAAAG 
AAACCAAGCT TACAAACCAA TGCCAAGGCC TTCAATGAAA AGCTACCTAA CAATGAAGAC 
AGAGTGCGGG GGAGCTTCGC CTTGGACTCA CCGCATCACT ACACCCCTAT TGAGGGGACG 
CCGTACTGCT TTTCCCGAAA TGACTCCTTG AGTTCTCTGG ATTTTGATGA TGACGATGTT 
GACCTTTCCA GGGAAAAGGC CGAGTTAAGA AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC 
AAAGTTACCT GCCGCCCAGA ACCAAACTCA AGCCAGCAGG CAGCTAGTAA GTCACAAGCC 
AGTATAAAAC ATCCAGCAAA CAGAGCACAG TCCAAACCAG TGCTGCAGAA ACAGCCCACT 
TTCCCCCAGT CCTCCAAAGA CGGACCAGAT AGAGGGGCAG CAACTGACGA AAAACTGCAG 
AATTTTGCTA TTGAAAATAC TCCAGTTTGC TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT 
AGTGACATTG ACCAGGAAAA CAACAATAAC AAAGAAAGTG AACCAATCAA AGAAGCTGAA 
CCTGCCAACT CACAAGGAGA GCCCAGTAAG CCTCAGGCAT CCGGGTATGC TCCCAAGTCC 
TTCCACGTCG AAGACACCCC TGTCTGTTTC TCAAGAAACA GCTCTCTCAG TTCTCTTAGC 
ATTGACTCTG AGGACGACCT GTTACAGGAG TGTATAAGTT CTGCCATGCC AAAAAAGAAA 
AGGCCTTCAA GACTCAAGAG TGAGAGCGAA AAGCAGAGCC CTAGAAAAGT GGGTGGCATA 
TTAGCTGAAG ACCTGACGCT TGATTTGAAA GATCTACAGA GGCCAGATTC AGAACACGCT 
TTCTCCCCCG ACTCAGAAAA TTTTGACTGG AAAGCTATTC AGGAAGGCGC AAACTCCATA 
GTAAGTAGTT TGCACCAAGC TGCTGCAGCC GCCGCGTGCT TATCTAGACA AGCGTCATCC 
GACTCAGATT CCATTCTGTC ACTAAAGTCC GGCATTTCTC TGGGATCGCC TTTTCATCTT 
ACACCTGATC AAGAGGAAAA GCCATTCACA AGCAATAAAG GCCCAAGAAT TCTCAAACCT 
GGAGAGAAAA GCACATTAGA AGCAAAAAAA ATAGAATCTG AAAACAAAGG AATCAAAGGC 
GGGAAAAAGG TTTATAAAAG CTTGATTACG GGAAAGATTC GCTCCAATTC AGAAATTTCC 
AGCCAAATGA AACAACCCCT CCCGACAAAC ATGCCTTCAA TCTCAAGAGG CAGGACGATG 
ATTCACATCC CAGGGCTTCG GAATAGCTCC TCTAGTACAA GCCCTGTCTC TAAGAAAGGC 
CCACCCCTCA AGACTCCAGC CTCTAAAAGC CCCAGTGAAG GGCCGGGAGC TACCACTTCT 
CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA GAGCTTAGCC CTATCACCAG GCAAACTTCC 
CAAATCAGTG GGTCAAATAA GGGGTCTTCT AGATCAGGAT CTAGAGACTC CACTCCCTCA 
AGACCTACAC AGCAACCATT AAGTAGGCCA ATGCAGTCTC CAGGGCGAAA CTCAATTTCC 
CCTGGTAGAA ATGGAATAAG CCCTCCTAAC AAACTGTCTC AGCTGCCCAG AACATCATCT 
CCCAGTACTG CTTCAACTAA GTCCTCCGGT TCTGGGAAAA TGTCATATAC ATCCCCAGGT 
AGACAGCTGA GCCAACAAAA TCTTACCAAA CAAGCAAGTT TATCCAAGAA TGCCAGCAGT 
ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA CTGAATCAGA TGAGTAACGG CAATGGGTCA 
AATAAAAAGG TAGAACTTTC TAGAATGTCT TCAACTAAAT CAAGTGGAAG TGAATCAGAC 
AGATCAGAAA GGCCTGCATT AGTACGCCAG TCTACTTTCA TCAAAGAAGC CCCAAGCCCA 
ACCCTGAGGA GGAAACTGGA GGAATCTGCC TCATTTGAAT CCCTTTCTCC ATCTTCTAGA 
CCAGATTCTC CCACCAGGTC GCAGGCACAG ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT 
ATGTCTCTGT CCACACATCC ATCTGTTCAG GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT 
CTCAGCCCCA CTATCGAGTA TAATGACGGA AGGCCCACAA AACGGCATGA TATTGCACGC 
TCCCATTCTG AAAGTCCTTC CAGACTACCA ATCAACCGGG CGGGAACCTG GAAGCGTGAA 
CACAGCAAAC ATTCCTCGTC CCTTCCTCGA GTGAGTACTT GGAGAAGAAC TGGAAGCTCA 
TCTTCTATTC TTTCTGCTTC ATCAGAGTCC AGTGAAAAAG CAAAAAGTGA GGATGAAAGG 
CATGTGAGCT CCATGCCAGC ACCCAGACAG ATGAAGGAAA ACCAGGTGCC CACCAAAGGA 
ACATGGAGGA AAATCAAGGA AAGTGACATT TCTCCCACAG GCATGGCTTC TCAGAGCGCT 
TCCTCAGGTG CTGCCAGTGG TGCTGAATCC AAGCCTCTGA TCTATCAGAT GGCACCTCCT 
GTCTCTAAAA CAGAGGATGT TTGGGTGAGA ATTGAGGACT GCCCCATTAA CAACCCTAGA 
TCTGGACGGT CCCCCACAGG CAACACCCCC CCAGTGATTG ACAGTGTTTC AGAGAAGGGA 
AGTTCAAGCA TTAAAGATTC AAAAGACACC CATGGGAAAC AGAGTGTGGG CAGTGGCAGT 
CCTGTGCAAA CCGTGGGTCT GGAAACCCGC CTCAACTCCT TTGTTCAGGT AGAGGCCCCA 
GAACAGAAAG GAACTGAGGC AAAACCAGGA CAGAGTAACC CAGTCTCTAT AGCAGAGACT 
GCTGAGACGT GTATAGCAGA GCGTACCCCT TTCAGTTCCA GTAGCTCCAG CAAGCACAGC 
TCACCTAGCG GGACTGTTGC TGCCAGAGTG ACACCTTTTA ATTACAACCC TAGCCCTAGG 
AAGAGCAGCG CAGACAGCAC TTCAGCCCGG CCGTCTCAGA TCCCTACGCC AGTGAGCACC 
AACACGAAGA AGAGAGATTC GAAGACTGAC AGCACAGAAT CCAGTGGAGC CCAAAGTCCT 
AAACGCCATT CCGGGTCTTA CCTCGTGACG TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525595.1
CDS	496..9054
Translation	MYAFLRSGPVAALPASVPPVTLGARSSGGGRGRVPGEGRRPGGARACGRGASVWQEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525532.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525532.3

ORF Insert Sequence:

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGGTG 
GAAATGGTGT ATTCCTTGTT GTCAATGCTT GGTACTCATG ATAAGGACGA TATGTCACGA 
ACTTTGCTAG CTATGTCCAG CTCCCAAGAC AGCTGTATAT CCATGCGGCA GTCTGGATGT 
CTTCCTCTCC TCATCCAGCT TTTACATGGC AATGACAAAG ACTCTGTATT GTTGGGAAAT 
TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG GCCAGTGCAG CACTCCACAA CATCATTCAC 
TCACAGCCTG ATGACAAGAG AGGCAGGCGT GAAATCCGAG TCCTTCATCT TTTGGAACAG 
ATACGAGCTT ACTGTGAAAC CTGTTGGGAG TGGCAGGAAG CCCACGAACA AGGCATGGAC 
CAGGACAAAA ACCCAATGCC AGCTCCTGTT GAGCATCAGA TCTGTCCTGC TGTGTGTGTT 
CTAATGAAGC TTTCATTTGA TGAAGAGCAT AGGCATGCAA TGAATGAACT TGGGGGACTG 
CAGGCCATTG CAGAGTTATT GCAGGTGGAC TGTGAGATGT ATGGGCTTAC TAATGACCAC 
TACAGTGTTA CTTTAAGACG GTATGCTGGA ATGGCTTTGA CAAACTTGAC CTTTGGAGAT 
GTTGCCAACA AGGCTACGCT GTGTTCTATG AAAGGCTGCA TGAGAGCACT TGTGGCCCAG 
TTAAAATCTG AGAGTGAAGA CTTACAGCAG GTTATTGCAA GTGTTTTGAG GAATTTGTCT 
TGGCGAGCAG ATGTAAATAG CAAAAAGACG TTGAGAGAAG TTGGAAGTGT GAAAGCATTG 
ATGGAATGTG CTTTGGAAGT TAAAAAGGAA TCAACCCTCA AAAGCGTTTT GAGTGCCTTA 
TGGAACCTGT CTGCACACTG CACTGAGAAT AAGGCTGACA TCTGTGCTGT GGATGGAGCA 
CTGGCATTTC TGGTTGGCAC CCTCACTTAC CGGAGCCAGA CAAATACTTT AGCCATTATT 
GAAAGTGGAG GTGGGATATT ACGGAATGTG TCCAGCTTGA TAGCTACAAA CGAAGACCAC 
AGGCAAATCC TAAGAGAGAA CAATTGCCTA CAAACTTTAT TACAGCACTT GAAATCTCAC 
AGCTTGACAA TAGTCAGTAA TGCATGTGGA ACTTTGTGGA ATCTCTCAGC AAGAAATCCT 
AAAGACCAGG AAGCCTTGTG GGACATGGGG GCAGTGAGCA TGCTCAAGAA CCTCATTCAT 
TCCAAGCACA AAATGATTGC CATGGGAAGT GCAGCAGCTT TAAGGAATCT CATGGCAAAC 
AGACCTGCAA AGTATAAGGA TGCCAATATC ATGTCTCCCG GCTCAAGTCT GCCATCCCTT 
CACGTTAGGA AACAGAAAGC TCTAGAAGCT GAGCTAGATG CTCAGCATTT ATCAGAAACC 
TTCGACAACA TTGACAACCT AAGTCCCAAG GCCTCTCACC GGAGTAAGCA GAGACACAAG 
CAGAATCTTT ATGGTGACTA TGCTTTTGAC GCCAATCGAC ATGATGATAG TAGGTCAGAC 
AATTTCAATA CTGGAAACAT GACTGTTCTT TCACCATATT TAAATACTAC GGTATTGCCC 
AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG 
GAGAGAGAGC GAGGTATTGG CCTCAGTGCT TACCATCCAA CAACAGAAAA TGCAGGAACC 
TCATCAAAAC GAGGTCTGCA GATCACTACC ACTGCAGCCC AGATAGCCAA AGTTATGGAA 
GAAGTATCAG CCATTCATAC CTCCCAGGAC GACAGAAGTT CTGCTTCTAC CACCGAGTTC 
CATTGTGTGG CAGACGACAG GAGTGCGGCA CGAAGAAGCT CTGCCTCCCA CACACACTCA 
AACACATACA ACTTCACTAA GTCGGAAAAT TCAAATAGGA CATGCTCTAT GCCTTATGCC 
AAAGTGGAAT ATAAACGATC TTCAAATGAC AGTTTAAATA GTGTCACTAG TAGTGATGGA 
TATGGTAAAA GAGGCCAAAT GAAACCCTCA GTTGAATCCT ATTCTGAAGA TGATGAAAGT 
AAATTTTGCA GTTATGGTCA GTATCCAGCT GACCTAGCCC ATAAGATACA CAGTGCAAAT 
CATATGGATG ATAATGATGG AGAACTGGAT ACACCAATAA ATTACAGTCT TAAATATTCA 
GATGAGCAGT TGAACTCAGG AAGGCAGAGT CCCTCACAGA ATGAAAGGTG GGCAAGACCA 
AAGCATGTGA TAGAAGATGA AATAAAGCAA AACGAGCAAA GACAAGCAAG AAGCCAGAAC 
ACCAGTTATC CTGTCTATTC TGAGAATACC GATGACAAAC ACCTCAAATT CCAACCACAT 
TTTGGACAAC AAGAATGTGT TTCCCCATAT AGGTCAAGGG GAACCAGTGG TTCAGAAACA 
AATCGAATGG GTTCTAGTCA TGCAATTAAT CAAAATGTAA ACCAGTCTCT GTGTCAGGAA 
GATGATTATG AAGATGATAA ACCTACCAAC TACAGTGAAC GTTATTCTGA GGAAGAACAA 
CATGAAGAAG AAGAAGAGAG ACCGACAAAT TATAGCATAA AATATAATGA AGAGAAACAT 
CATGTGGATC AGCCTATTGA TTATAGTTTA AAATATGCCA CTGACATTTC TTCCTCACAA 
AAACCATCAT TTTCATTCTC AAAGAATTCA TCAGCACAAA GCACTAAACC TGAACATCTC 
TCTCCAAGCA GCGAGAATAC AGCTGTACCT CCATCTAATG CCAAAAGGCA GAATCAGCTG 
CGTCCAAGTT CAGCACAAAG AAATGGCCAG ACTCAAAAAG GCACTACTTG CAAAGTCCCC 
TCCATCAACC AAGAAACAAT ACAGACTTAC TGCGTAGAAG ACACCCCAAT ATGTTTTTCA 
AGGTGCAGTT CATTATCATC ACTGTCATCA GCTGACGATG AAATAGGATG TGATCAGACA 
ACACAGGAAG CAGATTCTGC TAATACTCTG CAGACAGCAG AAGTAAAAGA GAATGATGTA 
ACTCGGTCAG CTGAAGATCC TGCAACTGAA GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC 
AAACCCAGCC GACTCCAGGC TTCTGGCTTA TCTTCAGAAT CAACCAGGCA TAATAAAGCT 
GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT 
CCCCCAGAAC ACTATGTCCA GGAGACTCCG CTCGTATTCA GCAGGTGTAC TTCTGTCAGC 
TCCCTTGACA GTTTTGAGAG TCGCTCCATT GCCAGCTCTG TTCAGAGTGA GCCATGTAGT 
GGAATGGTGA GTGGCATCAT AAGCCCCAGT GACCTTCCAG ATAGTCCTGG GCAGACCATG 
CCACCAAGCA GAAGCAAAAC CCCTCCACCT CCTCCACAGA CAGTGCAGGC CAAGAGAGAG 
GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG AAGAGAGAGA GTGGGCCTAA GCAGACTGCT 
GTAAATGCTG CCGTGCAGAG GGTGCAGGTC CTTCCAGACG TGGATACTTT GTTACACTTC 
GCCACAGAAA GTACTCCAGA CGGGTTTTCT TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG 
GATGAGCCAT TTATACAGAA AGATGTAGAA TTAAGAATCA TGCCTCCAGT TCAGGAAAAC 
GACAATGGGA ATGAAACTGA ATCAGAACAG CCTGAGGAAT CAAATGAAAA CCAGGATAAA 
GAGGTAGAAA AGCCTGACTC TGAAAAAGAC TTATTAGATG ATTCTGATGA CGATGATATT 
GAAATATTAG AAGAATGTAT TATTTCAGCC ATGCCAACAA AGTCATCACG CAAAGCCAAA 
AAACTAGCCC AGACTGCTTC AAAATTACCT CCACCTGTGG CAAGGAAACC AAGTCAGCTA 
CCTGTGTATA AACTTCTGCC AGCACAGAAT AGGCTGCAGG CACAAAAACA TGTTAGCTTT 
ACACCAGGGG ATGATGTGCC CCGGGTGTAC TGTGTAGAAG GGACACCTAT AAACTTTTCC 
ACAGCAACGT CTCTAAGTGA TCTGACAATA GAGTCCCCTC CAAATGAATT GGCTACTGGA 
GATGGGGTCA GAGCGGGTAT ACAGTCAGGT GAATTTGAAA AACGAGATAC CATTCCTACA 
GAAGGCAGAA GTACAGATGA TGCTCAGCGA GGAAAAATCT CATCTATAGT TACACCAGAC 
CTGGATGACA ACAAAGCAGA GGAAGGAGAT ATTCTTGCAG AATGTATCAA TTCTGCTATG 
CCCAAAGGAA AAAGCCACAA GCCTTTCCGA GTGAAAAAGA TAATGGACCA AGTCCAACAA 
GCATCCTCGA CTTCATCTGG AGCTAACAAA AATCAAGTAG ACACTAAGAA AAAGAAGCCT 
ACTTCACCAG TAAAGCCCAT GCCACAAAAT ACTGAATATA GAACGCGTGT GAGAAAGAAT 
ACAGACTCAA AAGTTAATGT AAATACTGAA GAAACTTTCT CAGACAACAA AGACTCAAAG 
AAACCAAGCT TACAAACCAA TGCCAAGGCC TTCAATGAAA AGCTACCTAA CAATGAAGAC 
AGAGTGCGGG GGAGCTTCGC CTTGGACTCA CCGCATCACT ACACCCCTAT TGAGGGGACG 
CCGTACTGCT TTTCCCGAAA TGACTCCTTG AGTTCTCTGG ATTTTGATGA TGACGATGTT 
GACCTTTCCA GGGAAAAGGC CGAGTTAAGA AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC 
AAAGTTACCT GCCGCCCAGA ACCAAACTCA AGCCAGCAGG CAGCTAGTAA GTCACAAGCC 
AGTATAAAAC ATCCAGCAAA CAGAGCACAG TCCAAACCAG TGCTGCAGAA ACAGCCCACT 
TTCCCCCAGT CCTCCAAAGA CGGACCAGAT AGAGGGGCAG CAACTGACGA AAAACTGCAG 
AATTTTGCTA TTGAAAATAC TCCAGTTTGC TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT 
AGTGACATTG ACCAGGAAAA CAACAATAAC AAAGAAAGTG AACCAATCAA AGAAGCTGAA 
CCTGCCAACT CACAAGGAGA GCCCAGTAAG CCTCAGGCAT CCGGGTATGC TCCCAAGTCC 
TTCCACGTCG AAGACACCCC TGTCTGTTTC TCAAGAAACA GCTCTCTCAG TTCTCTTAGC 
ATTGACTCTG AGGACGACCT GTTACAGGAG TGTATAAGTT CTGCCATGCC AAAAAAGAAA 
AGGCCTTCAA GACTCAAGAG TGAGAGCGAA AAGCAGAGCC CTAGAAAAGT GGGTGGCATA 
TTAGCTGAAG ACCTGACGCT TGATTTGAAA GATCTACAGA GGCCAGATTC AGAACACGCT 
TTCTCCCCCG ACTCAGAAAA TTTTGACTGG AAAGCTATTC AGGAAGGCGC AAACTCCATA 
GTAAGTAGTT TGCACCAAGC TGCTGCAGCC GCCGCGTGCT TATCTAGACA AGCGTCATCC 
GACTCAGATT CCATTCTGTC ACTAAAGTCC GGCATTTCTC TGGGATCGCC TTTTCATCTT 
ACACCTGATC AAGAGGAAAA GCCATTCACA AGCAATAAAG GCCCAAGAAT TCTCAAACCT 
GGAGAGAAAA GCACATTAGA AGCAAAAAAA ATAGAATCTG AAAACAAAGG AATCAAAGGC 
GGGAAAAAGG TTTATAAAAG CTTGATTACG GGAAAGATTC GCTCCAATTC AGAAATTTCC 
AGCCAAATGA AACAACCCCT CCCGACAAAC ATGCCTTCAA TCTCAAGAGG CAGGACGATG 
ATTCACATCC CAGGGCTTCG GAATAGCTCC TCTAGTACAA GCCCTGTCTC TAAGAAAGGC 
CCACCCCTCA AGACTCCAGC CTCTAAAAGC CCCAGTGAAG GGCCGGGAGC TACCACTTCT 
CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA GAGCTTAGCC CTATCACCAG GCAAACTTCC 
CAAATCAGTG GGTCAAATAA GGGGTCTTCT AGATCAGGAT CTAGAGACTC CACTCCCTCA 
AGACCTACAC AGCAACCATT AAGTAGGCCA ATGCAGTCTC CAGGGCGAAA CTCAATTTCC 
CCTGGTAGAA ATGGAATAAG CCCTCCTAAC AAACTGTCTC AGCTGCCCAG AACATCATCT 
CCCAGTACTG CTTCAACTAA GTCCTCCGGT TCTGGGAAAA TGTCATATAC ATCCCCAGGT 
AGACAGCTGA GCCAACAAAA TCTTACCAAA CAAGCAAGTT TATCCAAGAA TGCCAGCAGT 
ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA CTGAATCAGA TGAGTAACGG CAATGGGTCA 
AATAAAAAGG TAGAACTTTC TAGAATGTCT TCAACTAAAT CAAGTGGAAG TGAATCAGAC 
AGATCAGAAA GGCCTGCATT AGTACGCCAG TCTACTTTCA TCAAAGAAGC CCCAAGCCCA 
ACCCTGAGGA GGAAACTGGA GGAATCTGCC TCATTTGAAT CCCTTTCTCC ATCTTCTAGA 
CCAGATTCTC CCACCAGGTC GCAGGCACAG ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT 
ATGTCTCTGT CCACACATCC ATCTGTTCAG GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT 
CTCAGCCCCA CTATCGAGTA TAATGACGGA AGGCCCACAA AACGGCATGA TATTGCACGC 
TCCCATTCTG AAAGTCCTTC CAGACTACCA ATCAACCGGG CGGGAACCTG GAAGCGTGAA 
CACAGCAAAC ATTCCTCGTC CCTTCCTCGA GTGAGTACTT GGAGAAGAAC TGGAAGCTCA 
TCTTCTATTC TTTCTGCTTC ATCAGAGTCC AGTGAAAAAG CAAAAAGTGA GGATGAAAGG 
CATGTGAGCT CCATGCCAGC ACCCAGACAG ATGAAGGAAA ACCAGGTGCC CACCAAAGGA 
ACATGGAGGA AAATCAAGGA AAGTGACATT TCTCCCACAG GCATGGCTTC TCAGAGCGCT 
TCCTCAGGTG CTGCCAGTGG TGCTGAATCC AAGCCTCTGA TCTATCAGAT GGCACCTCCT 
GTCTCTAAAA CAGAGGATGT TTGGGTGAGA ATTGAGGACT GCCCCATTAA CAACCCTAGA 
TCTGGACGGT CCCCCACAGG CAACACCCCC CCAGTGATTG ACAGTGTTTC AGAGAAGGGA 
AGTTCAAGCA TTAAAGATTC AAAAGACACC CATGGGAAAC AGAGTGTGGG CAGTGGCAGT 
CCTGTGCAAA CCGTGGGTCT GGAAACCCGC CTCAACTCCT TTGTTCAGGT AGAGGCCCCA 
GAACAGAAAG GAACTGAGGC AAAACCAGGA CAGAGTAACC CAGTCTCTAT AGCAGAGACT 
GCTGAGACGT GTATAGCAGA GCGTACCCCT TTCAGTTCCA GTAGCTCCAG CAAGCACAGC 
TCACCTAGCG GGACTGTTGC TGCCAGAGTG ACACCTTTTA ATTACAACCC TAGCCCTAGG 
AAGAGCAGCG CAGACAGCAC TTCAGCCCGG CCGTCTCAGA TCCCTACGCC AGTGAGCACC 
AACACGAAGA AGAGAGATTC GAAGACTGAC AGCACAGAAT CCAGTGGAGC CCAAAGTCCT 
AAACGCCATT CCGGGTCTTA CCTCGTGACG TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525532.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525595.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X3, mRNA.

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CloneID	OMu54319
Clone ID Related Accession (Same CDS sequence)	XM_006525532.3 , XM_006525532.4
Accession Version	XM_006525532.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525532.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGGTG 
GAAATGGTGT ATTCCTTGTT GTCAATGCTT GGTACTCATG ATAAGGACGA TATGTCACGA 
ACTTTGCTAG CTATGTCCAG CTCCCAAGAC AGCTGTATAT CCATGCGGCA GTCTGGATGT 
CTTCCTCTCC TCATCCAGCT TTTACATGGC AATGACAAAG ACTCTGTATT GTTGGGAAAT 
TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG GCCAGTGCAG CACTCCACAA CATCATTCAC 
TCACAGCCTG ATGACAAGAG AGGCAGGCGT GAAATCCGAG TCCTTCATCT TTTGGAACAG 
ATACGAGCTT ACTGTGAAAC CTGTTGGGAG TGGCAGGAAG CCCACGAACA AGGCATGGAC 
CAGGACAAAA ACCCAATGCC AGCTCCTGTT GAGCATCAGA TCTGTCCTGC TGTGTGTGTT 
CTAATGAAGC TTTCATTTGA TGAAGAGCAT AGGCATGCAA TGAATGAACT TGGGGGACTG 
CAGGCCATTG CAGAGTTATT GCAGGTGGAC TGTGAGATGT ATGGGCTTAC TAATGACCAC 
TACAGTGTTA CTTTAAGACG GTATGCTGGA ATGGCTTTGA CAAACTTGAC CTTTGGAGAT 
GTTGCCAACA AGGCTACGCT GTGTTCTATG AAAGGCTGCA TGAGAGCACT TGTGGCCCAG 
TTAAAATCTG AGAGTGAAGA CTTACAGCAG GTTATTGCAA GTGTTTTGAG GAATTTGTCT 
TGGCGAGCAG ATGTAAATAG CAAAAAGACG TTGAGAGAAG TTGGAAGTGT GAAAGCATTG 
ATGGAATGTG CTTTGGAAGT TAAAAAGGAA TCAACCCTCA AAAGCGTTTT GAGTGCCTTA 
TGGAACCTGT CTGCACACTG CACTGAGAAT AAGGCTGACA TCTGTGCTGT GGATGGAGCA 
CTGGCATTTC TGGTTGGCAC CCTCACTTAC CGGAGCCAGA CAAATACTTT AGCCATTATT 
GAAAGTGGAG GTGGGATATT ACGGAATGTG TCCAGCTTGA TAGCTACAAA CGAAGACCAC 
AGGCAAATCC TAAGAGAGAA CAATTGCCTA CAAACTTTAT TACAGCACTT GAAATCTCAC 
AGCTTGACAA TAGTCAGTAA TGCATGTGGA ACTTTGTGGA ATCTCTCAGC AAGAAATCCT 
AAAGACCAGG AAGCCTTGTG GGACATGGGG GCAGTGAGCA TGCTCAAGAA CCTCATTCAT 
TCCAAGCACA AAATGATTGC CATGGGAAGT GCAGCAGCTT TAAGGAATCT CATGGCAAAC 
AGACCTGCAA AGTATAAGGA TGCCAATATC ATGTCTCCCG GCTCAAGTCT GCCATCCCTT 
CACGTTAGGA AACAGAAAGC TCTAGAAGCT GAGCTAGATG CTCAGCATTT ATCAGAAACC 
TTCGACAACA TTGACAACCT AAGTCCCAAG GCCTCTCACC GGAGTAAGCA GAGACACAAG 
CAGAATCTTT ATGGTGACTA TGCTTTTGAC GCCAATCGAC ATGATGATAG TAGGTCAGAC 
AATTTCAATA CTGGAAACAT GACTGTTCTT TCACCATATT TAAATACTAC GGTATTGCCC 
AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG 
GAGAGAGAGC GAGGTATTGG CCTCAGTGCT TACCATCCAA CAACAGAAAA TGCAGGAACC 
TCATCAAAAC GAGGTCTGCA GATCACTACC ACTGCAGCCC AGATAGCCAA AGTTATGGAA 
GAAGTATCAG CCATTCATAC CTCCCAGGAC GACAGAAGTT CTGCTTCTAC CACCGAGTTC 
CATTGTGTGG CAGACGACAG GAGTGCGGCA CGAAGAAGCT CTGCCTCCCA CACACACTCA 
AACACATACA ACTTCACTAA GTCGGAAAAT TCAAATAGGA CATGCTCTAT GCCTTATGCC 
AAAGTGGAAT ATAAACGATC TTCAAATGAC AGTTTAAATA GTGTCACTAG TAGTGATGGA 
TATGGTAAAA GAGGCCAAAT GAAACCCTCA GTTGAATCCT ATTCTGAAGA TGATGAAAGT 
AAATTTTGCA GTTATGGTCA GTATCCAGCT GACCTAGCCC ATAAGATACA CAGTGCAAAT 
CATATGGATG ATAATGATGG AGAACTGGAT ACACCAATAA ATTACAGTCT TAAATATTCA 
GATGAGCAGT TGAACTCAGG AAGGCAGAGT CCCTCACAGA ATGAAAGGTG GGCAAGACCA 
AAGCATGTGA TAGAAGATGA AATAAAGCAA AACGAGCAAA GACAAGCAAG AAGCCAGAAC 
ACCAGTTATC CTGTCTATTC TGAGAATACC GATGACAAAC ACCTCAAATT CCAACCACAT 
TTTGGACAAC AAGAATGTGT TTCCCCATAT AGGTCAAGGG GAACCAGTGG TTCAGAAACA 
AATCGAATGG GTTCTAGTCA TGCAATTAAT CAAAATGTAA ACCAGTCTCT GTGTCAGGAA 
GATGATTATG AAGATGATAA ACCTACCAAC TACAGTGAAC GTTATTCTGA GGAAGAACAA 
CATGAAGAAG AAGAAGAGAG ACCGACAAAT TATAGCATAA AATATAATGA AGAGAAACAT 
CATGTGGATC AGCCTATTGA TTATAGTTTA AAATATGCCA CTGACATTTC TTCCTCACAA 
AAACCATCAT TTTCATTCTC AAAGAATTCA TCAGCACAAA GCACTAAACC TGAACATCTC 
TCTCCAAGCA GCGAGAATAC AGCTGTACCT CCATCTAATG CCAAAAGGCA GAATCAGCTG 
CGTCCAAGTT CAGCACAAAG AAATGGCCAG ACTCAAAAAG GCACTACTTG CAAAGTCCCC 
TCCATCAACC AAGAAACAAT ACAGACTTAC TGCGTAGAAG ACACCCCAAT ATGTTTTTCA 
AGGTGCAGTT CATTATCATC ACTGTCATCA GCTGACGATG AAATAGGATG TGATCAGACA 
ACACAGGAAG CAGATTCTGC TAATACTCTG CAGACAGCAG AAGTAAAAGA GAATGATGTA 
ACTCGGTCAG CTGAAGATCC TGCAACTGAA GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC 
AAACCCAGCC GACTCCAGGC TTCTGGCTTA TCTTCAGAAT CAACCAGGCA TAATAAAGCT 
GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT 
CCCCCAGAAC ACTATGTCCA GGAGACTCCG CTCGTATTCA GCAGGTGTAC TTCTGTCAGC 
TCCCTTGACA GTTTTGAGAG TCGCTCCATT GCCAGCTCTG TTCAGAGTGA GCCATGTAGT 
GGAATGGTGA GTGGCATCAT AAGCCCCAGT GACCTTCCAG ATAGTCCTGG GCAGACCATG 
CCACCAAGCA GAAGCAAAAC CCCTCCACCT CCTCCACAGA CAGTGCAGGC CAAGAGAGAG 
GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG AAGAGAGAGA GTGGGCCTAA GCAGACTGCT 
GTAAATGCTG CCGTGCAGAG GGTGCAGGTC CTTCCAGACG TGGATACTTT GTTACACTTC 
GCCACAGAAA GTACTCCAGA CGGGTTTTCT TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG 
GATGAGCCAT TTATACAGAA AGATGTAGAA TTAAGAATCA TGCCTCCAGT TCAGGAAAAC 
GACAATGGGA ATGAAACTGA ATCAGAACAG CCTGAGGAAT CAAATGAAAA CCAGGATAAA 
GAGGTAGAAA AGCCTGACTC TGAAAAAGAC TTATTAGATG ATTCTGATGA CGATGATATT 
GAAATATTAG AAGAATGTAT TATTTCAGCC ATGCCAACAA AGTCATCACG CAAAGCCAAA 
AAACTAGCCC AGACTGCTTC AAAATTACCT CCACCTGTGG CAAGGAAACC AAGTCAGCTA 
CCTGTGTATA AACTTCTGCC AGCACAGAAT AGGCTGCAGG CACAAAAACA TGTTAGCTTT 
ACACCAGGGG ATGATGTGCC CCGGGTGTAC TGTGTAGAAG GGACACCTAT AAACTTTTCC 
ACAGCAACGT CTCTAAGTGA TCTGACAATA GAGTCCCCTC CAAATGAATT GGCTACTGGA 
GATGGGGTCA GAGCGGGTAT ACAGTCAGGT GAATTTGAAA AACGAGATAC CATTCCTACA 
GAAGGCAGAA GTACAGATGA TGCTCAGCGA GGAAAAATCT CATCTATAGT TACACCAGAC 
CTGGATGACA ACAAAGCAGA GGAAGGAGAT ATTCTTGCAG AATGTATCAA TTCTGCTATG 
CCCAAAGGAA AAAGCCACAA GCCTTTCCGA GTGAAAAAGA TAATGGACCA AGTCCAACAA 
GCATCCTCGA CTTCATCTGG AGCTAACAAA AATCAAGTAG ACACTAAGAA AAAGAAGCCT 
ACTTCACCAG TAAAGCCCAT GCCACAAAAT ACTGAATATA GAACGCGTGT GAGAAAGAAT 
ACAGACTCAA AAGTTAATGT AAATACTGAA GAAACTTTCT CAGACAACAA AGACTCAAAG 
AAACCAAGCT TACAAACCAA TGCCAAGGCC TTCAATGAAA AGCTACCTAA CAATGAAGAC 
AGAGTGCGGG GGAGCTTCGC CTTGGACTCA CCGCATCACT ACACCCCTAT TGAGGGGACG 
CCGTACTGCT TTTCCCGAAA TGACTCCTTG AGTTCTCTGG ATTTTGATGA TGACGATGTT 
GACCTTTCCA GGGAAAAGGC CGAGTTAAGA AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC 
AAAGTTACCT GCCGCCCAGA ACCAAACTCA AGCCAGCAGG CAGCTAGTAA GTCACAAGCC 
AGTATAAAAC ATCCAGCAAA CAGAGCACAG TCCAAACCAG TGCTGCAGAA ACAGCCCACT 
TTCCCCCAGT CCTCCAAAGA CGGACCAGAT AGAGGGGCAG CAACTGACGA AAAACTGCAG 
AATTTTGCTA TTGAAAATAC TCCAGTTTGC TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT 
AGTGACATTG ACCAGGAAAA CAACAATAAC AAAGAAAGTG AACCAATCAA AGAAGCTGAA 
CCTGCCAACT CACAAGGAGA GCCCAGTAAG CCTCAGGCAT CCGGGTATGC TCCCAAGTCC 
TTCCACGTCG AAGACACCCC TGTCTGTTTC TCAAGAAACA GCTCTCTCAG TTCTCTTAGC 
ATTGACTCTG AGGACGACCT GTTACAGGAG TGTATAAGTT CTGCCATGCC AAAAAAGAAA 
AGGCCTTCAA GACTCAAGAG TGAGAGCGAA AAGCAGAGCC CTAGAAAAGT GGGTGGCATA 
TTAGCTGAAG ACCTGACGCT TGATTTGAAA GATCTACAGA GGCCAGATTC AGAACACGCT 
TTCTCCCCCG ACTCAGAAAA TTTTGACTGG AAAGCTATTC AGGAAGGCGC AAACTCCATA 
GTAAGTAGTT TGCACCAAGC TGCTGCAGCC GCCGCGTGCT TATCTAGACA AGCGTCATCC 
GACTCAGATT CCATTCTGTC ACTAAAGTCC GGCATTTCTC TGGGATCGCC TTTTCATCTT 
ACACCTGATC AAGAGGAAAA GCCATTCACA AGCAATAAAG GCCCAAGAAT TCTCAAACCT 
GGAGAGAAAA GCACATTAGA AGCAAAAAAA ATAGAATCTG AAAACAAAGG AATCAAAGGC 
GGGAAAAAGG TTTATAAAAG CTTGATTACG GGAAAGATTC GCTCCAATTC AGAAATTTCC 
AGCCAAATGA AACAACCCCT CCCGACAAAC ATGCCTTCAA TCTCAAGAGG CAGGACGATG 
ATTCACATCC CAGGGCTTCG GAATAGCTCC TCTAGTACAA GCCCTGTCTC TAAGAAAGGC 
CCACCCCTCA AGACTCCAGC CTCTAAAAGC CCCAGTGAAG GGCCGGGAGC TACCACTTCT 
CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA GAGCTTAGCC CTATCACCAG GCAAACTTCC 
CAAATCAGTG GGTCAAATAA GGGGTCTTCT AGATCAGGAT CTAGAGACTC CACTCCCTCA 
AGACCTACAC AGCAACCATT AAGTAGGCCA ATGCAGTCTC CAGGGCGAAA CTCAATTTCC 
CCTGGTAGAA ATGGAATAAG CCCTCCTAAC AAACTGTCTC AGCTGCCCAG AACATCATCT 
CCCAGTACTG CTTCAACTAA GTCCTCCGGT TCTGGGAAAA TGTCATATAC ATCCCCAGGT 
AGACAGCTGA GCCAACAAAA TCTTACCAAA CAAGCAAGTT TATCCAAGAA TGCCAGCAGT 
ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA CTGAATCAGA TGAGTAACGG CAATGGGTCA 
AATAAAAAGG TAGAACTTTC TAGAATGTCT TCAACTAAAT CAAGTGGAAG TGAATCAGAC 
AGATCAGAAA GGCCTGCATT AGTACGCCAG TCTACTTTCA TCAAAGAAGC CCCAAGCCCA 
ACCCTGAGGA GGAAACTGGA GGAATCTGCC TCATTTGAAT CCCTTTCTCC ATCTTCTAGA 
CCAGATTCTC CCACCAGGTC GCAGGCACAG ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT 
ATGTCTCTGT CCACACATCC ATCTGTTCAG GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT 
CTCAGCCCCA CTATCGAGTA TAATGACGGA AGGCCCACAA AACGGCATGA TATTGCACGC 
TCCCATTCTG AAAGTCCTTC CAGACTACCA ATCAACCGGG CGGGAACCTG GAAGCGTGAA 
CACAGCAAAC ATTCCTCGTC CCTTCCTCGA GTGAGTACTT GGAGAAGAAC TGGAAGCTCA 
TCTTCTATTC TTTCTGCTTC ATCAGAGTCC AGTGAAAAAG CAAAAAGTGA GGATGAAAGG 
CATGTGAGCT CCATGCCAGC ACCCAGACAG ATGAAGGAAA ACCAGGTGCC CACCAAAGGA 
ACATGGAGGA AAATCAAGGA AAGTGACATT TCTCCCACAG GCATGGCTTC TCAGAGCGCT 
TCCTCAGGTG CTGCCAGTGG TGCTGAATCC AAGCCTCTGA TCTATCAGAT GGCACCTCCT 
GTCTCTAAAA CAGAGGATGT TTGGGTGAGA ATTGAGGACT GCCCCATTAA CAACCCTAGA 
TCTGGACGGT CCCCCACAGG CAACACCCCC CCAGTGATTG ACAGTGTTTC AGAGAAGGGA 
AGTTCAAGCA TTAAAGATTC AAAAGACACC CATGGGAAAC AGAGTGTGGG CAGTGGCAGT 
CCTGTGCAAA CCGTGGGTCT GGAAACCCGC CTCAACTCCT TTGTTCAGGT AGAGGCCCCA 
GAACAGAAAG GAACTGAGGC AAAACCAGGA CAGAGTAACC CAGTCTCTAT AGCAGAGACT 
GCTGAGACGT GTATAGCAGA GCGTACCCCT TTCAGTTCCA GTAGCTCCAG CAAGCACAGC 
TCACCTAGCG GGACTGTTGC TGCCAGAGTG ACACCTTTTA ATTACAACCC TAGCCCTAGG 
AAGAGCAGCG CAGACAGCAC TTCAGCCCGG CCGTCTCAGA TCCCTACGCC AGTGAGCACC 
AACACGAAGA AGAGAGATTC GAAGACTGAC AGCACAGAAT CCAGTGGAGC CCAAAGTCCT 
AAACGCCATT CCGGGTCTTA CCTCGTGACG TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525595.1
CDS	816..9374
Translation	MYAFLRSGPVAALPASVPPVTLGARSSGGGRGRVPGEGRRPGGARACGRGASVWQEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525532.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525532.4

ORF Insert Sequence:

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGGTG 
GAAATGGTGT ATTCCTTGTT GTCAATGCTT GGTACTCATG ATAAGGACGA TATGTCACGA 
ACTTTGCTAG CTATGTCCAG CTCCCAAGAC AGCTGTATAT CCATGCGGCA GTCTGGATGT 
CTTCCTCTCC TCATCCAGCT TTTACATGGC AATGACAAAG ACTCTGTATT GTTGGGAAAT 
TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG GCCAGTGCAG CACTCCACAA CATCATTCAC 
TCACAGCCTG ATGACAAGAG AGGCAGGCGT GAAATCCGAG TCCTTCATCT TTTGGAACAG 
ATACGAGCTT ACTGTGAAAC CTGTTGGGAG TGGCAGGAAG CCCACGAACA AGGCATGGAC 
CAGGACAAAA ACCCAATGCC AGCTCCTGTT GAGCATCAGA TCTGTCCTGC TGTGTGTGTT 
CTAATGAAGC TTTCATTTGA TGAAGAGCAT AGGCATGCAA TGAATGAACT TGGGGGACTG 
CAGGCCATTG CAGAGTTATT GCAGGTGGAC TGTGAGATGT ATGGGCTTAC TAATGACCAC 
TACAGTGTTA CTTTAAGACG GTATGCTGGA ATGGCTTTGA CAAACTTGAC CTTTGGAGAT 
GTTGCCAACA AGGCTACGCT GTGTTCTATG AAAGGCTGCA TGAGAGCACT TGTGGCCCAG 
TTAAAATCTG AGAGTGAAGA CTTACAGCAG GTTATTGCAA GTGTTTTGAG GAATTTGTCT 
TGGCGAGCAG ATGTAAATAG CAAAAAGACG TTGAGAGAAG TTGGAAGTGT GAAAGCATTG 
ATGGAATGTG CTTTGGAAGT TAAAAAGGAA TCAACCCTCA AAAGCGTTTT GAGTGCCTTA 
TGGAACCTGT CTGCACACTG CACTGAGAAT AAGGCTGACA TCTGTGCTGT GGATGGAGCA 
CTGGCATTTC TGGTTGGCAC CCTCACTTAC CGGAGCCAGA CAAATACTTT AGCCATTATT 
GAAAGTGGAG GTGGGATATT ACGGAATGTG TCCAGCTTGA TAGCTACAAA CGAAGACCAC 
AGGCAAATCC TAAGAGAGAA CAATTGCCTA CAAACTTTAT TACAGCACTT GAAATCTCAC 
AGCTTGACAA TAGTCAGTAA TGCATGTGGA ACTTTGTGGA ATCTCTCAGC AAGAAATCCT 
AAAGACCAGG AAGCCTTGTG GGACATGGGG GCAGTGAGCA TGCTCAAGAA CCTCATTCAT 
TCCAAGCACA AAATGATTGC CATGGGAAGT GCAGCAGCTT TAAGGAATCT CATGGCAAAC 
AGACCTGCAA AGTATAAGGA TGCCAATATC ATGTCTCCCG GCTCAAGTCT GCCATCCCTT 
CACGTTAGGA AACAGAAAGC TCTAGAAGCT GAGCTAGATG CTCAGCATTT ATCAGAAACC 
TTCGACAACA TTGACAACCT AAGTCCCAAG GCCTCTCACC GGAGTAAGCA GAGACACAAG 
CAGAATCTTT ATGGTGACTA TGCTTTTGAC GCCAATCGAC ATGATGATAG TAGGTCAGAC 
AATTTCAATA CTGGAAACAT GACTGTTCTT TCACCATATT TAAATACTAC GGTATTGCCC 
AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG 
GAGAGAGAGC GAGGTATTGG CCTCAGTGCT TACCATCCAA CAACAGAAAA TGCAGGAACC 
TCATCAAAAC GAGGTCTGCA GATCACTACC ACTGCAGCCC AGATAGCCAA AGTTATGGAA 
GAAGTATCAG CCATTCATAC CTCCCAGGAC GACAGAAGTT CTGCTTCTAC CACCGAGTTC 
CATTGTGTGG CAGACGACAG GAGTGCGGCA CGAAGAAGCT CTGCCTCCCA CACACACTCA 
AACACATACA ACTTCACTAA GTCGGAAAAT TCAAATAGGA CATGCTCTAT GCCTTATGCC 
AAAGTGGAAT ATAAACGATC TTCAAATGAC AGTTTAAATA GTGTCACTAG TAGTGATGGA 
TATGGTAAAA GAGGCCAAAT GAAACCCTCA GTTGAATCCT ATTCTGAAGA TGATGAAAGT 
AAATTTTGCA GTTATGGTCA GTATCCAGCT GACCTAGCCC ATAAGATACA CAGTGCAAAT 
CATATGGATG ATAATGATGG AGAACTGGAT ACACCAATAA ATTACAGTCT TAAATATTCA 
GATGAGCAGT TGAACTCAGG AAGGCAGAGT CCCTCACAGA ATGAAAGGTG GGCAAGACCA 
AAGCATGTGA TAGAAGATGA AATAAAGCAA AACGAGCAAA GACAAGCAAG AAGCCAGAAC 
ACCAGTTATC CTGTCTATTC TGAGAATACC GATGACAAAC ACCTCAAATT CCAACCACAT 
TTTGGACAAC AAGAATGTGT TTCCCCATAT AGGTCAAGGG GAACCAGTGG TTCAGAAACA 
AATCGAATGG GTTCTAGTCA TGCAATTAAT CAAAATGTAA ACCAGTCTCT GTGTCAGGAA 
GATGATTATG AAGATGATAA ACCTACCAAC TACAGTGAAC GTTATTCTGA GGAAGAACAA 
CATGAAGAAG AAGAAGAGAG ACCGACAAAT TATAGCATAA AATATAATGA AGAGAAACAT 
CATGTGGATC AGCCTATTGA TTATAGTTTA AAATATGCCA CTGACATTTC TTCCTCACAA 
AAACCATCAT TTTCATTCTC AAAGAATTCA TCAGCACAAA GCACTAAACC TGAACATCTC 
TCTCCAAGCA GCGAGAATAC AGCTGTACCT CCATCTAATG CCAAAAGGCA GAATCAGCTG 
CGTCCAAGTT CAGCACAAAG AAATGGCCAG ACTCAAAAAG GCACTACTTG CAAAGTCCCC 
TCCATCAACC AAGAAACAAT ACAGACTTAC TGCGTAGAAG ACACCCCAAT ATGTTTTTCA 
AGGTGCAGTT CATTATCATC ACTGTCATCA GCTGACGATG AAATAGGATG TGATCAGACA 
ACACAGGAAG CAGATTCTGC TAATACTCTG CAGACAGCAG AAGTAAAAGA GAATGATGTA 
ACTCGGTCAG CTGAAGATCC TGCAACTGAA GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC 
AAACCCAGCC GACTCCAGGC TTCTGGCTTA TCTTCAGAAT CAACCAGGCA TAATAAAGCT 
GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT 
CCCCCAGAAC ACTATGTCCA GGAGACTCCG CTCGTATTCA GCAGGTGTAC TTCTGTCAGC 
TCCCTTGACA GTTTTGAGAG TCGCTCCATT GCCAGCTCTG TTCAGAGTGA GCCATGTAGT 
GGAATGGTGA GTGGCATCAT AAGCCCCAGT GACCTTCCAG ATAGTCCTGG GCAGACCATG 
CCACCAAGCA GAAGCAAAAC CCCTCCACCT CCTCCACAGA CAGTGCAGGC CAAGAGAGAG 
GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG AAGAGAGAGA GTGGGCCTAA GCAGACTGCT 
GTAAATGCTG CCGTGCAGAG GGTGCAGGTC CTTCCAGACG TGGATACTTT GTTACACTTC 
GCCACAGAAA GTACTCCAGA CGGGTTTTCT TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG 
GATGAGCCAT TTATACAGAA AGATGTAGAA TTAAGAATCA TGCCTCCAGT TCAGGAAAAC 
GACAATGGGA ATGAAACTGA ATCAGAACAG CCTGAGGAAT CAAATGAAAA CCAGGATAAA 
GAGGTAGAAA AGCCTGACTC TGAAAAAGAC TTATTAGATG ATTCTGATGA CGATGATATT 
GAAATATTAG AAGAATGTAT TATTTCAGCC ATGCCAACAA AGTCATCACG CAAAGCCAAA 
AAACTAGCCC AGACTGCTTC AAAATTACCT CCACCTGTGG CAAGGAAACC AAGTCAGCTA 
CCTGTGTATA AACTTCTGCC AGCACAGAAT AGGCTGCAGG CACAAAAACA TGTTAGCTTT 
ACACCAGGGG ATGATGTGCC CCGGGTGTAC TGTGTAGAAG GGACACCTAT AAACTTTTCC 
ACAGCAACGT CTCTAAGTGA TCTGACAATA GAGTCCCCTC CAAATGAATT GGCTACTGGA 
GATGGGGTCA GAGCGGGTAT ACAGTCAGGT GAATTTGAAA AACGAGATAC CATTCCTACA 
GAAGGCAGAA GTACAGATGA TGCTCAGCGA GGAAAAATCT CATCTATAGT TACACCAGAC 
CTGGATGACA ACAAAGCAGA GGAAGGAGAT ATTCTTGCAG AATGTATCAA TTCTGCTATG 
CCCAAAGGAA AAAGCCACAA GCCTTTCCGA GTGAAAAAGA TAATGGACCA AGTCCAACAA 
GCATCCTCGA CTTCATCTGG AGCTAACAAA AATCAAGTAG ACACTAAGAA AAAGAAGCCT 
ACTTCACCAG TAAAGCCCAT GCCACAAAAT ACTGAATATA GAACGCGTGT GAGAAAGAAT 
ACAGACTCAA AAGTTAATGT AAATACTGAA GAAACTTTCT CAGACAACAA AGACTCAAAG 
AAACCAAGCT TACAAACCAA TGCCAAGGCC TTCAATGAAA AGCTACCTAA CAATGAAGAC 
AGAGTGCGGG GGAGCTTCGC CTTGGACTCA CCGCATCACT ACACCCCTAT TGAGGGGACG 
CCGTACTGCT TTTCCCGAAA TGACTCCTTG AGTTCTCTGG ATTTTGATGA TGACGATGTT 
GACCTTTCCA GGGAAAAGGC CGAGTTAAGA AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC 
AAAGTTACCT GCCGCCCAGA ACCAAACTCA AGCCAGCAGG CAGCTAGTAA GTCACAAGCC 
AGTATAAAAC ATCCAGCAAA CAGAGCACAG TCCAAACCAG TGCTGCAGAA ACAGCCCACT 
TTCCCCCAGT CCTCCAAAGA CGGACCAGAT AGAGGGGCAG CAACTGACGA AAAACTGCAG 
AATTTTGCTA TTGAAAATAC TCCAGTTTGC TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT 
AGTGACATTG ACCAGGAAAA CAACAATAAC AAAGAAAGTG AACCAATCAA AGAAGCTGAA 
CCTGCCAACT CACAAGGAGA GCCCAGTAAG CCTCAGGCAT CCGGGTATGC TCCCAAGTCC 
TTCCACGTCG AAGACACCCC TGTCTGTTTC TCAAGAAACA GCTCTCTCAG TTCTCTTAGC 
ATTGACTCTG AGGACGACCT GTTACAGGAG TGTATAAGTT CTGCCATGCC AAAAAAGAAA 
AGGCCTTCAA GACTCAAGAG TGAGAGCGAA AAGCAGAGCC CTAGAAAAGT GGGTGGCATA 
TTAGCTGAAG ACCTGACGCT TGATTTGAAA GATCTACAGA GGCCAGATTC AGAACACGCT 
TTCTCCCCCG ACTCAGAAAA TTTTGACTGG AAAGCTATTC AGGAAGGCGC AAACTCCATA 
GTAAGTAGTT TGCACCAAGC TGCTGCAGCC GCCGCGTGCT TATCTAGACA AGCGTCATCC 
GACTCAGATT CCATTCTGTC ACTAAAGTCC GGCATTTCTC TGGGATCGCC TTTTCATCTT 
ACACCTGATC AAGAGGAAAA GCCATTCACA AGCAATAAAG GCCCAAGAAT TCTCAAACCT 
GGAGAGAAAA GCACATTAGA AGCAAAAAAA ATAGAATCTG AAAACAAAGG AATCAAAGGC 
GGGAAAAAGG TTTATAAAAG CTTGATTACG GGAAAGATTC GCTCCAATTC AGAAATTTCC 
AGCCAAATGA AACAACCCCT CCCGACAAAC ATGCCTTCAA TCTCAAGAGG CAGGACGATG 
ATTCACATCC CAGGGCTTCG GAATAGCTCC TCTAGTACAA GCCCTGTCTC TAAGAAAGGC 
CCACCCCTCA AGACTCCAGC CTCTAAAAGC CCCAGTGAAG GGCCGGGAGC TACCACTTCT 
CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA GAGCTTAGCC CTATCACCAG GCAAACTTCC 
CAAATCAGTG GGTCAAATAA GGGGTCTTCT AGATCAGGAT CTAGAGACTC CACTCCCTCA 
AGACCTACAC AGCAACCATT AAGTAGGCCA ATGCAGTCTC CAGGGCGAAA CTCAATTTCC 
CCTGGTAGAA ATGGAATAAG CCCTCCTAAC AAACTGTCTC AGCTGCCCAG AACATCATCT 
CCCAGTACTG CTTCAACTAA GTCCTCCGGT TCTGGGAAAA TGTCATATAC ATCCCCAGGT 
AGACAGCTGA GCCAACAAAA TCTTACCAAA CAAGCAAGTT TATCCAAGAA TGCCAGCAGT 
ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA CTGAATCAGA TGAGTAACGG CAATGGGTCA 
AATAAAAAGG TAGAACTTTC TAGAATGTCT TCAACTAAAT CAAGTGGAAG TGAATCAGAC 
AGATCAGAAA GGCCTGCATT AGTACGCCAG TCTACTTTCA TCAAAGAAGC CCCAAGCCCA 
ACCCTGAGGA GGAAACTGGA GGAATCTGCC TCATTTGAAT CCCTTTCTCC ATCTTCTAGA 
CCAGATTCTC CCACCAGGTC GCAGGCACAG ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT 
ATGTCTCTGT CCACACATCC ATCTGTTCAG GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT 
CTCAGCCCCA CTATCGAGTA TAATGACGGA AGGCCCACAA AACGGCATGA TATTGCACGC 
TCCCATTCTG AAAGTCCTTC CAGACTACCA ATCAACCGGG CGGGAACCTG GAAGCGTGAA 
CACAGCAAAC ATTCCTCGTC CCTTCCTCGA GTGAGTACTT GGAGAAGAAC TGGAAGCTCA 
TCTTCTATTC TTTCTGCTTC ATCAGAGTCC AGTGAAAAAG CAAAAAGTGA GGATGAAAGG 
CATGTGAGCT CCATGCCAGC ACCCAGACAG ATGAAGGAAA ACCAGGTGCC CACCAAAGGA 
ACATGGAGGA AAATCAAGGA AAGTGACATT TCTCCCACAG GCATGGCTTC TCAGAGCGCT 
TCCTCAGGTG CTGCCAGTGG TGCTGAATCC AAGCCTCTGA TCTATCAGAT GGCACCTCCT 
GTCTCTAAAA CAGAGGATGT TTGGGTGAGA ATTGAGGACT GCCCCATTAA CAACCCTAGA 
TCTGGACGGT CCCCCACAGG CAACACCCCC CCAGTGATTG ACAGTGTTTC AGAGAAGGGA 
AGTTCAAGCA TTAAAGATTC AAAAGACACC CATGGGAAAC AGAGTGTGGG CAGTGGCAGT 
CCTGTGCAAA CCGTGGGTCT GGAAACCCGC CTCAACTCCT TTGTTCAGGT AGAGGCCCCA 
GAACAGAAAG GAACTGAGGC AAAACCAGGA CAGAGTAACC CAGTCTCTAT AGCAGAGACT 
GCTGAGACGT GTATAGCAGA GCGTACCCCT TTCAGTTCCA GTAGCTCCAG CAAGCACAGC 
TCACCTAGCG GGACTGTTGC TGCCAGAGTG ACACCTTTTA ATTACAACCC TAGCCCTAGG 
AAGAGCAGCG CAGACAGCAC TTCAGCCCGG CCGTCTCAGA TCCCTACGCC AGTGAGCACC 
AACACGAAGA AGAGAGATTC GAAGACTGAC AGCACAGAAT CCAGTGGAGC CCAAAGTCCT 
AAACGCCATT CCGGGTCTTA CCTCGTGACG TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525533.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525596.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X5, mRNA.

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CloneID	OMu54320
Clone ID Related Accession (Same CDS sequence)	XM_006525533.3 , XM_006525533.2
Accession Version	XM_006525533.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525533.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGT ATTGGCGCAG CTTGCCGGGG TGTCACTGGA GACAGAATGG AGGTGCTGCC 
GGGCTCGAAA ATGGGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGTAGGAAG GCTACCCGGG GCATTTCATC CCAGGAGCTA 
GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC ATTGCAGAGT TATTGCAGGT GGACTGTGAG 
ATGTATGGGC TTACTAATGA CCACTACAGT GTTACTTTAA GACGGTATGC TGGAATGGCT 
TTGACAAACT TGACCTTTGG AGATGTTGCC AACAAGGCTA CGCTGTGTTC TATGAAAGGC 
TGCATGAGAG CACTTGTGGC CCAGTTAAAA TCTGAGAGTG AAGACTTACA GCAGGTTATT 
GCAAGTGTTT TGAGGAATTT GTCTTGGCGA GCAGATGTAA ATAGCAAAAA GACGTTGAGA 
GAAGTTGGAA GTGTGAAAGC ATTGATGGAA TGTGCTTTGG AAGTTAAAAA GGAATCAACC 
CTCAAAAGCG TTTTGAGTGC CTTATGGAAC CTGTCTGCAC ACTGCACTGA GAATAAGGCT 
GACATCTGTG CTGTGGATGG AGCACTGGCA TTTCTGGTTG GCACCCTCAC TTACCGGAGC 
CAGACAAATA CTTTAGCCAT TATTGAAAGT GGAGGTGGGA TATTACGGAA TGTGTCCAGC 
TTGATAGCTA CAAACGAAGA CCACAGGCAA ATCCTAAGAG AGAACAATTG CCTACAAACT 
TTATTACAGC ACTTGAAATC TCACAGCTTG ACAATAGTCA GTAATGCATG TGGAACTTTG 
TGGAATCTCT CAGCAAGAAA TCCTAAAGAC CAGGAAGCCT TGTGGGACAT GGGGGCAGTG 
AGCATGCTCA AGAACCTCAT TCATTCCAAG CACAAAATGA TTGCCATGGG AAGTGCAGCA 
GCTTTAAGGA ATCTCATGGC AAACAGACCT GCAAAGTATA AGGATGCCAA TATCATGTCT 
CCCGGCTCAA GTCTGCCATC CCTTCACGTT AGGAAACAGA AAGCTCTAGA AGCTGAGCTA 
GATGCTCAGC ATTTATCAGA AACCTTCGAC AACATTGACA ACCTAAGTCC CAAGGCCTCT 
CACCGGAGTA AGCAGAGACA CAAGCAGAAT CTTTATGGTG ACTATGCTTT TGACGCCAAT 
CGACATGATG ATAGTAGGTC AGACAATTTC AATACTGGAA ACATGACTGT TCTTTCACCA 
TATTTAAATA CTACGGTATT GCCCAGCTCT TCTTCCTCAA GGGGAAGTTT AGACAGTTCT 
CGTTCTGAGA AAGACAGAAG TTTGGAGAGA GAGCGAGGTA TTGGCCTCAG TGCTTACCAT 
CCAACAACAG AAAATGCAGG AACCTCATCA AAACGAGGTC TGCAGATCAC TACCACTGCA 
GCCCAGATAG CCAAAGTTAT GGAAGAAGTA TCAGCCATTC ATACCTCCCA GGACGACAGA 
AGTTCTGCTT CTACCACCGA GTTCCATTGT GTGGCAGACG ACAGGAGTGC GGCACGAAGA 
AGCTCTGCCT CCCACACACA CTCAAACACA TACAACTTCA CTAAGTCGGA AAATTCAAAT 
AGGACATGCT CTATGCCTTA TGCCAAAGTG GAATATAAAC GATCTTCAAA TGACAGTTTA 
AATAGTGTCA CTAGTAGTGA TGGATATGGT AAAAGAGGCC AAATGAAACC CTCAGTTGAA 
TCCTATTCTG AAGATGATGA AAGTAAATTT TGCAGTTATG GTCAGTATCC AGCTGACCTA 
GCCCATAAGA TACACAGTGC AAATCATATG GATGATAATG ATGGAGAACT GGATACACCA 
ATAAATTACA GTCTTAAATA TTCAGATGAG CAGTTGAACT CAGGAAGGCA GAGTCCCTCA 
CAGAATGAAA GGTGGGCAAG ACCAAAGCAT GTGATAGAAG ATGAAATAAA GCAAAACGAG 
CAAAGACAAG CAAGAAGCCA GAACACCAGT TATCCTGTCT ATTCTGAGAA TACCGATGAC 
AAACACCTCA AATTCCAACC ACATTTTGGA CAACAAGAAT GTGTTTCCCC ATATAGGTCA 
AGGGGAACCA GTGGTTCAGA AACAAATCGA ATGGGTTCTA GTCATGCAAT TAATCAAAAT 
GTAAACCAGT CTCTGTGTCA GGAAGATGAT TATGAAGATG ATAAACCTAC CAACTACAGT 
GAACGTTATT CTGAGGAAGA ACAACATGAA GAAGAAGAAG AGAGACCGAC AAATTATAGC 
ATAAAATATA ATGAAGAGAA ACATCATGTG GATCAGCCTA TTGATTATAG TTTAAAATAT 
GCCACTGACA TTTCTTCCTC ACAAAAACCA TCATTTTCAT TCTCAAAGAA TTCATCAGCA 
CAAAGCACTA AACCTGAACA TCTCTCTCCA AGCAGCGAGA ATACAGCTGT ACCTCCATCT 
AATGCCAAAA GGCAGAATCA GCTGCGTCCA AGTTCAGCAC AAAGAAATGG CCAGACTCAA 
AAAGGCACTA CTTGCAAAGT CCCCTCCATC AACCAAGAAA CAATACAGAC TTACTGCGTA 
GAAGACACCC CAATATGTTT TTCAAGGTGC AGTTCATTAT CATCACTGTC ATCAGCTGAC 
GATGAAATAG GATGTGATCA GACAACACAG GAAGCAGATT CTGCTAATAC TCTGCAGACA 
GCAGAAGTAA AAGAGAATGA TGTAACTCGG TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA 
GCAGTGTCCC AGAATGCTAG AGCCAAACCC AGCCGACTCC AGGCTTCTGG CTTATCTTCA 
GAATCAACCA GGCATAATAA AGCTGTTGAG TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA 
AGTGGTGCTC AGACACCCAA AAGTCCCCCA GAACACTATG TCCAGGAGAC TCCGCTCGTA 
TTCAGCAGGT GTACTTCTGT CAGCTCCCTT GACAGTTTTG AGAGTCGCTC CATTGCCAGC 
TCTGTTCAGA GTGAGCCATG TAGTGGAATG GTGAGTGGCA TCATAAGCCC CAGTGACCTT 
CCAGATAGTC CTGGGCAGAC CATGCCACCA AGCAGAAGCA AAACCCCTCC ACCTCCTCCA 
CAGACAGTGC AGGCCAAGAG AGAGGTGCCA AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA 
GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA 
GACGTGGATA CTTTGTTACA CTTCGCCACA GAAAGTACTC CAGACGGGTT TTCTTGTTCC 
TCCAGCCTAA GTGCTCTGAG CCTGGATGAG CCATTTATAC AGAAAGATGT AGAATTAAGA 
ATCATGCCTC CAGTTCAGGA AAACGACAAT GGGAATGAAA CTGAATCAGA ACAGCCTGAG 
GAATCAAATG AAAACCAGGA TAAAGAGGTA GAAAAGCCTG ACTCTGAAAA AGACTTATTA 
GATGATTCTG ATGACGATGA TATTGAAATA TTAGAAGAAT GTATTATTTC AGCCATGCCA 
ACAAAGTCAT CACGCAAAGC CAAAAAACTA GCCCAGACTG CTTCAAAATT ACCTCCACCT 
GTGGCAAGGA AACCAAGTCA GCTACCTGTG TATAAACTTC TGCCAGCACA GAATAGGCTG 
CAGGCACAAA AACATGTTAG CTTTACACCA GGGGATGATG TGCCCCGGGT GTACTGTGTA 
GAAGGGACAC CTATAAACTT TTCCACAGCA ACGTCTCTAA GTGATCTGAC AATAGAGTCC 
CCTCCAAATG AATTGGCTAC TGGAGATGGG GTCAGAGCGG GTATACAGTC AGGTGAATTT 
GAAAAACGAG ATACCATTCC TACAGAAGGC AGAAGTACAG ATGATGCTCA GCGAGGAAAA 
ATCTCATCTA TAGTTACACC AGACCTGGAT GACAACAAAG CAGAGGAAGG AGATATTCTT 
GCAGAATGTA TCAATTCTGC TATGCCCAAA GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA 
AAGATAATGG ACCAAGTCCA ACAAGCATCC TCGACTTCAT CTGGAGCTAA CAAAAATCAA 
GTAGACACTA AGAAAAAGAA GCCTACTTCA CCAGTAAAGC CCATGCCACA AAATACTGAA 
TATAGAACGC GTGTGAGAAA GAATACAGAC TCAAAAGTTA ATGTAAATAC TGAAGAAACT 
TTCTCAGACA ACAAAGACTC AAAGAAACCA AGCTTACAAA CCAATGCCAA GGCCTTCAAT 
GAAAAGCTAC CTAACAATGA AGACAGAGTG CGGGGGAGCT TCGCCTTGGA CTCACCGCAT 
CACTACACCC CTATTGAGGG GACGCCGTAC TGCTTTTCCC GAAATGACTC CTTGAGTTCT 
CTGGATTTTG ATGATGACGA TGTTGACCTT TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC 
AAAGAAAGCA AGGATTCCGA AGCCAAAGTT ACCTGCCGCC CAGAACCAAA CTCAAGCCAG 
CAGGCAGCTA GTAAGTCACA AGCCAGTATA AAACATCCAG CAAACAGAGC ACAGTCCAAA 
CCAGTGCTGC AGAAACAGCC CACTTTCCCC CAGTCCTCCA AAGACGGACC AGATAGAGGG 
GCAGCAACTG ACGAAAAACT GCAGAATTTT GCTATTGAAA ATACTCCAGT TTGCTTTTCT 
CGAAATTCCT CTCTGAGTTC CCTTAGTGAC ATTGACCAGG AAAACAACAA TAACAAAGAA 
AGTGAACCAA TCAAAGAAGC TGAACCTGCC AACTCACAAG GAGAGCCCAG TAAGCCTCAG 
GCATCCGGGT ATGCTCCCAA GTCCTTCCAC GTCGAAGACA CCCCTGTCTG TTTCTCAAGA 
AACAGCTCTC TCAGTTCTCT TAGCATTGAC TCTGAGGACG ACCTGTTACA GGAGTGTATA 
AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT TCAAGACTCA AGAGTGAGAG CGAAAAGCAG 
AGCCCTAGAA AAGTGGGTGG CATATTAGCT GAAGACCTGA CGCTTGATTT GAAAGATCTA 
CAGAGGCCAG ATTCAGAACA CGCTTTCTCC CCCGACTCAG AAAATTTTGA CTGGAAAGCT 
ATTCAGGAAG GCGCAAACTC CATAGTAAGT AGTTTGCACC AAGCTGCTGC AGCCGCCGCG 
TGCTTATCTA GACAAGCGTC ATCCGACTCA GATTCCATTC TGTCACTAAA GTCCGGCATT 
TCTCTGGGAT CGCCTTTTCA TCTTACACCT GATCAAGAGG AAAAGCCATT CACAAGCAAT 
AAAGGCCCAA GAATTCTCAA ACCTGGAGAG AAAAGCACAT TAGAAGCAAA AAAAATAGAA 
TCTGAAAACA AAGGAATCAA AGGCGGGAAA AAGGTTTATA AAAGCTTGAT TACGGGAAAG 
ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA ATGAAACAAC CCCTCCCGAC AAACATGCCT 
TCAATCTCAA GAGGCAGGAC GATGATTCAC ATCCCAGGGC TTCGGAATAG CTCCTCTAGT 
ACAAGCCCTG TCTCTAAGAA AGGCCCACCC CTCAAGACTC CAGCCTCTAA AAGCCCCAGT 
GAAGGGCCGG GAGCTACCAC TTCTCCTCGA GGAACTAAGC CAGCAGGAAA GTCAGAGCTT 
AGCCCTATCA CCAGGCAAAC TTCCCAAATC AGTGGGTCAA ATAAGGGGTC TTCTAGATCA 
GGATCTAGAG ACTCCACTCC CTCAAGACCT ACACAGCAAC CATTAAGTAG GCCAATGCAG 
TCTCCAGGGC GAAACTCAAT TTCCCCTGGT AGAAATGGAA TAAGCCCTCC TAACAAACTG 
TCTCAGCTGC CCAGAACATC ATCTCCCAGT ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG 
AAAATGTCAT ATACATCCCC AGGTAGACAG CTGAGCCAAC AAAATCTTAC CAAACAAGCA 
AGTTTATCCA AGAATGCCAG CAGTATCCCC AGAAGTGAGT CGGCATCTAA AGGACTGAAT 
CAGATGAGTA ACGGCAATGG GTCAAATAAA AAGGTAGAAC TTTCTAGAAT GTCTTCAACT 
AAATCAAGTG GAAGTGAATC AGACAGATCA GAAAGGCCTG CATTAGTACG CCAGTCTACT 
TTCATCAAAG AAGCCCCAAG CCCAACCCTG AGGAGGAAAC TGGAGGAATC TGCCTCATTT 
GAATCCCTTT CTCCATCTTC TAGACCAGAT TCTCCCACCA GGTCGCAGGC ACAGACCCCA 
GTTTTAAGCC CTTCCCTTCC TGATATGTCT CTGTCCACAC ATCCATCTGT TCAGGCAGGT 
GGGTGGCGAA AGCTCCCGCC TAATCTCAGC CCCACTATCG AGTATAATGA CGGAAGGCCC 
ACAAAACGGC ATGATATTGC ACGCTCCCAT TCTGAAAGTC CTTCCAGACT ACCAATCAAC 
CGGGCGGGAA CCTGGAAGCG TGAACACAGC AAACATTCCT CGTCCCTTCC TCGAGTGAGT 
ACTTGGAGAA GAACTGGAAG CTCATCTTCT ATTCTTTCTG CTTCATCAGA GTCCAGTGAA 
AAAGCAAAAA GTGAGGATGA AAGGCATGTG AGCTCCATGC CAGCACCCAG ACAGATGAAG 
GAAAACCAGG TGCCCACCAA AGGAACATGG AGGAAAATCA AGGAAAGTGA CATTTCTCCC 
ACAGGCATGG CTTCTCAGAG CGCTTCCTCA GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT 
CTGATCTATC AGATGGCACC TCCTGTCTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG 
GACTGCCCCA TTAACAACCC TAGATCTGGA CGGTCCCCCA CAGGCAACAC CCCCCCAGTG 
ATTGACAGTG TTTCAGAGAA GGGAAGTTCA AGCATTAAAG ATTCAAAAGA CACCCATGGG 
AAACAGAGTG TGGGCAGTGG CAGTCCTGTG CAAACCGTGG GTCTGGAAAC CCGCCTCAAC 
TCCTTTGTTC AGGTAGAGGC CCCAGAACAG AAAGGAACTG AGGCAAAACC AGGACAGAGT 
AACCCAGTCT CTATAGCAGA GACTGCTGAG ACGTGTATAG CAGAGCGTAC CCCTTTCAGT 
TCCAGTAGCT CCAGCAAGCA CAGCTCACCT AGCGGGACTG TTGCTGCCAG AGTGACACCT 
TTTAATTACA ACCCTAGCCC TAGGAAGAGC AGCGCAGACA GCACTTCAGC CCGGCCGTCT 
CAGATCCCTA CGCCAGTGAG CACCAACACG AAGAAGAGAG ATTCGAAGAC TGACAGCACA 
GAATCCAGTG GAGCCCAAAG TCCTAAACGC CATTCCGGGT CTTACCTCGT GACGTCTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525596.1
CDS	133..8655
Translation	MWLYWRSLPGCHWRQNGGAAGLENGEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525533.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525533.3

ORF Insert Sequence:

ATGTGGCTGT ATTGGCGCAG CTTGCCGGGG TGTCACTGGA GACAGAATGG AGGTGCTGCC 
GGGCTCGAAA ATGGGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGTAGGAAG GCTACCCGGG GCATTTCATC CCAGGAGCTA 
GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC ATTGCAGAGT TATTGCAGGT GGACTGTGAG 
ATGTATGGGC TTACTAATGA CCACTACAGT GTTACTTTAA GACGGTATGC TGGAATGGCT 
TTGACAAACT TGACCTTTGG AGATGTTGCC AACAAGGCTA CGCTGTGTTC TATGAAAGGC 
TGCATGAGAG CACTTGTGGC CCAGTTAAAA TCTGAGAGTG AAGACTTACA GCAGGTTATT 
GCAAGTGTTT TGAGGAATTT GTCTTGGCGA GCAGATGTAA ATAGCAAAAA GACGTTGAGA 
GAAGTTGGAA GTGTGAAAGC ATTGATGGAA TGTGCTTTGG AAGTTAAAAA GGAATCAACC 
CTCAAAAGCG TTTTGAGTGC CTTATGGAAC CTGTCTGCAC ACTGCACTGA GAATAAGGCT 
GACATCTGTG CTGTGGATGG AGCACTGGCA TTTCTGGTTG GCACCCTCAC TTACCGGAGC 
CAGACAAATA CTTTAGCCAT TATTGAAAGT GGAGGTGGGA TATTACGGAA TGTGTCCAGC 
TTGATAGCTA CAAACGAAGA CCACAGGCAA ATCCTAAGAG AGAACAATTG CCTACAAACT 
TTATTACAGC ACTTGAAATC TCACAGCTTG ACAATAGTCA GTAATGCATG TGGAACTTTG 
TGGAATCTCT CAGCAAGAAA TCCTAAAGAC CAGGAAGCCT TGTGGGACAT GGGGGCAGTG 
AGCATGCTCA AGAACCTCAT TCATTCCAAG CACAAAATGA TTGCCATGGG AAGTGCAGCA 
GCTTTAAGGA ATCTCATGGC AAACAGACCT GCAAAGTATA AGGATGCCAA TATCATGTCT 
CCCGGCTCAA GTCTGCCATC CCTTCACGTT AGGAAACAGA AAGCTCTAGA AGCTGAGCTA 
GATGCTCAGC ATTTATCAGA AACCTTCGAC AACATTGACA ACCTAAGTCC CAAGGCCTCT 
CACCGGAGTA AGCAGAGACA CAAGCAGAAT CTTTATGGTG ACTATGCTTT TGACGCCAAT 
CGACATGATG ATAGTAGGTC AGACAATTTC AATACTGGAA ACATGACTGT TCTTTCACCA 
TATTTAAATA CTACGGTATT GCCCAGCTCT TCTTCCTCAA GGGGAAGTTT AGACAGTTCT 
CGTTCTGAGA AAGACAGAAG TTTGGAGAGA GAGCGAGGTA TTGGCCTCAG TGCTTACCAT 
CCAACAACAG AAAATGCAGG AACCTCATCA AAACGAGGTC TGCAGATCAC TACCACTGCA 
GCCCAGATAG CCAAAGTTAT GGAAGAAGTA TCAGCCATTC ATACCTCCCA GGACGACAGA 
AGTTCTGCTT CTACCACCGA GTTCCATTGT GTGGCAGACG ACAGGAGTGC GGCACGAAGA 
AGCTCTGCCT CCCACACACA CTCAAACACA TACAACTTCA CTAAGTCGGA AAATTCAAAT 
AGGACATGCT CTATGCCTTA TGCCAAAGTG GAATATAAAC GATCTTCAAA TGACAGTTTA 
AATAGTGTCA CTAGTAGTGA TGGATATGGT AAAAGAGGCC AAATGAAACC CTCAGTTGAA 
TCCTATTCTG AAGATGATGA AAGTAAATTT TGCAGTTATG GTCAGTATCC AGCTGACCTA 
GCCCATAAGA TACACAGTGC AAATCATATG GATGATAATG ATGGAGAACT GGATACACCA 
ATAAATTACA GTCTTAAATA TTCAGATGAG CAGTTGAACT CAGGAAGGCA GAGTCCCTCA 
CAGAATGAAA GGTGGGCAAG ACCAAAGCAT GTGATAGAAG ATGAAATAAA GCAAAACGAG 
CAAAGACAAG CAAGAAGCCA GAACACCAGT TATCCTGTCT ATTCTGAGAA TACCGATGAC 
AAACACCTCA AATTCCAACC ACATTTTGGA CAACAAGAAT GTGTTTCCCC ATATAGGTCA 
AGGGGAACCA GTGGTTCAGA AACAAATCGA ATGGGTTCTA GTCATGCAAT TAATCAAAAT 
GTAAACCAGT CTCTGTGTCA GGAAGATGAT TATGAAGATG ATAAACCTAC CAACTACAGT 
GAACGTTATT CTGAGGAAGA ACAACATGAA GAAGAAGAAG AGAGACCGAC AAATTATAGC 
ATAAAATATA ATGAAGAGAA ACATCATGTG GATCAGCCTA TTGATTATAG TTTAAAATAT 
GCCACTGACA TTTCTTCCTC ACAAAAACCA TCATTTTCAT TCTCAAAGAA TTCATCAGCA 
CAAAGCACTA AACCTGAACA TCTCTCTCCA AGCAGCGAGA ATACAGCTGT ACCTCCATCT 
AATGCCAAAA GGCAGAATCA GCTGCGTCCA AGTTCAGCAC AAAGAAATGG CCAGACTCAA 
AAAGGCACTA CTTGCAAAGT CCCCTCCATC AACCAAGAAA CAATACAGAC TTACTGCGTA 
GAAGACACCC CAATATGTTT TTCAAGGTGC AGTTCATTAT CATCACTGTC ATCAGCTGAC 
GATGAAATAG GATGTGATCA GACAACACAG GAAGCAGATT CTGCTAATAC TCTGCAGACA 
GCAGAAGTAA AAGAGAATGA TGTAACTCGG TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA 
GCAGTGTCCC AGAATGCTAG AGCCAAACCC AGCCGACTCC AGGCTTCTGG CTTATCTTCA 
GAATCAACCA GGCATAATAA AGCTGTTGAG TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA 
AGTGGTGCTC AGACACCCAA AAGTCCCCCA GAACACTATG TCCAGGAGAC TCCGCTCGTA 
TTCAGCAGGT GTACTTCTGT CAGCTCCCTT GACAGTTTTG AGAGTCGCTC CATTGCCAGC 
TCTGTTCAGA GTGAGCCATG TAGTGGAATG GTGAGTGGCA TCATAAGCCC CAGTGACCTT 
CCAGATAGTC CTGGGCAGAC CATGCCACCA AGCAGAAGCA AAACCCCTCC ACCTCCTCCA 
CAGACAGTGC AGGCCAAGAG AGAGGTGCCA AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA 
GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA 
GACGTGGATA CTTTGTTACA CTTCGCCACA GAAAGTACTC CAGACGGGTT TTCTTGTTCC 
TCCAGCCTAA GTGCTCTGAG CCTGGATGAG CCATTTATAC AGAAAGATGT AGAATTAAGA 
ATCATGCCTC CAGTTCAGGA AAACGACAAT GGGAATGAAA CTGAATCAGA ACAGCCTGAG 
GAATCAAATG AAAACCAGGA TAAAGAGGTA GAAAAGCCTG ACTCTGAAAA AGACTTATTA 
GATGATTCTG ATGACGATGA TATTGAAATA TTAGAAGAAT GTATTATTTC AGCCATGCCA 
ACAAAGTCAT CACGCAAAGC CAAAAAACTA GCCCAGACTG CTTCAAAATT ACCTCCACCT 
GTGGCAAGGA AACCAAGTCA GCTACCTGTG TATAAACTTC TGCCAGCACA GAATAGGCTG 
CAGGCACAAA AACATGTTAG CTTTACACCA GGGGATGATG TGCCCCGGGT GTACTGTGTA 
GAAGGGACAC CTATAAACTT TTCCACAGCA ACGTCTCTAA GTGATCTGAC AATAGAGTCC 
CCTCCAAATG AATTGGCTAC TGGAGATGGG GTCAGAGCGG GTATACAGTC AGGTGAATTT 
GAAAAACGAG ATACCATTCC TACAGAAGGC AGAAGTACAG ATGATGCTCA GCGAGGAAAA 
ATCTCATCTA TAGTTACACC AGACCTGGAT GACAACAAAG CAGAGGAAGG AGATATTCTT 
GCAGAATGTA TCAATTCTGC TATGCCCAAA GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA 
AAGATAATGG ACCAAGTCCA ACAAGCATCC TCGACTTCAT CTGGAGCTAA CAAAAATCAA 
GTAGACACTA AGAAAAAGAA GCCTACTTCA CCAGTAAAGC CCATGCCACA AAATACTGAA 
TATAGAACGC GTGTGAGAAA GAATACAGAC TCAAAAGTTA ATGTAAATAC TGAAGAAACT 
TTCTCAGACA ACAAAGACTC AAAGAAACCA AGCTTACAAA CCAATGCCAA GGCCTTCAAT 
GAAAAGCTAC CTAACAATGA AGACAGAGTG CGGGGGAGCT TCGCCTTGGA CTCACCGCAT 
CACTACACCC CTATTGAGGG GACGCCGTAC TGCTTTTCCC GAAATGACTC CTTGAGTTCT 
CTGGATTTTG ATGATGACGA TGTTGACCTT TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC 
AAAGAAAGCA AGGATTCCGA AGCCAAAGTT ACCTGCCGCC CAGAACCAAA CTCAAGCCAG 
CAGGCAGCTA GTAAGTCACA AGCCAGTATA AAACATCCAG CAAACAGAGC ACAGTCCAAA 
CCAGTGCTGC AGAAACAGCC CACTTTCCCC CAGTCCTCCA AAGACGGACC AGATAGAGGG 
GCAGCAACTG ACGAAAAACT GCAGAATTTT GCTATTGAAA ATACTCCAGT TTGCTTTTCT 
CGAAATTCCT CTCTGAGTTC CCTTAGTGAC ATTGACCAGG AAAACAACAA TAACAAAGAA 
AGTGAACCAA TCAAAGAAGC TGAACCTGCC AACTCACAAG GAGAGCCCAG TAAGCCTCAG 
GCATCCGGGT ATGCTCCCAA GTCCTTCCAC GTCGAAGACA CCCCTGTCTG TTTCTCAAGA 
AACAGCTCTC TCAGTTCTCT TAGCATTGAC TCTGAGGACG ACCTGTTACA GGAGTGTATA 
AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT TCAAGACTCA AGAGTGAGAG CGAAAAGCAG 
AGCCCTAGAA AAGTGGGTGG CATATTAGCT GAAGACCTGA CGCTTGATTT GAAAGATCTA 
CAGAGGCCAG ATTCAGAACA CGCTTTCTCC CCCGACTCAG AAAATTTTGA CTGGAAAGCT 
ATTCAGGAAG GCGCAAACTC CATAGTAAGT AGTTTGCACC AAGCTGCTGC AGCCGCCGCG 
TGCTTATCTA GACAAGCGTC ATCCGACTCA GATTCCATTC TGTCACTAAA GTCCGGCATT 
TCTCTGGGAT CGCCTTTTCA TCTTACACCT GATCAAGAGG AAAAGCCATT CACAAGCAAT 
AAAGGCCCAA GAATTCTCAA ACCTGGAGAG AAAAGCACAT TAGAAGCAAA AAAAATAGAA 
TCTGAAAACA AAGGAATCAA AGGCGGGAAA AAGGTTTATA AAAGCTTGAT TACGGGAAAG 
ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA ATGAAACAAC CCCTCCCGAC AAACATGCCT 
TCAATCTCAA GAGGCAGGAC GATGATTCAC ATCCCAGGGC TTCGGAATAG CTCCTCTAGT 
ACAAGCCCTG TCTCTAAGAA AGGCCCACCC CTCAAGACTC CAGCCTCTAA AAGCCCCAGT 
GAAGGGCCGG GAGCTACCAC TTCTCCTCGA GGAACTAAGC CAGCAGGAAA GTCAGAGCTT 
AGCCCTATCA CCAGGCAAAC TTCCCAAATC AGTGGGTCAA ATAAGGGGTC TTCTAGATCA 
GGATCTAGAG ACTCCACTCC CTCAAGACCT ACACAGCAAC CATTAAGTAG GCCAATGCAG 
TCTCCAGGGC GAAACTCAAT TTCCCCTGGT AGAAATGGAA TAAGCCCTCC TAACAAACTG 
TCTCAGCTGC CCAGAACATC ATCTCCCAGT ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG 
AAAATGTCAT ATACATCCCC AGGTAGACAG CTGAGCCAAC AAAATCTTAC CAAACAAGCA 
AGTTTATCCA AGAATGCCAG CAGTATCCCC AGAAGTGAGT CGGCATCTAA AGGACTGAAT 
CAGATGAGTA ACGGCAATGG GTCAAATAAA AAGGTAGAAC TTTCTAGAAT GTCTTCAACT 
AAATCAAGTG GAAGTGAATC AGACAGATCA GAAAGGCCTG CATTAGTACG CCAGTCTACT 
TTCATCAAAG AAGCCCCAAG CCCAACCCTG AGGAGGAAAC TGGAGGAATC TGCCTCATTT 
GAATCCCTTT CTCCATCTTC TAGACCAGAT TCTCCCACCA GGTCGCAGGC ACAGACCCCA 
GTTTTAAGCC CTTCCCTTCC TGATATGTCT CTGTCCACAC ATCCATCTGT TCAGGCAGGT 
GGGTGGCGAA AGCTCCCGCC TAATCTCAGC CCCACTATCG AGTATAATGA CGGAAGGCCC 
ACAAAACGGC ATGATATTGC ACGCTCCCAT TCTGAAAGTC CTTCCAGACT ACCAATCAAC 
CGGGCGGGAA CCTGGAAGCG TGAACACAGC AAACATTCCT CGTCCCTTCC TCGAGTGAGT 
ACTTGGAGAA GAACTGGAAG CTCATCTTCT ATTCTTTCTG CTTCATCAGA GTCCAGTGAA 
AAAGCAAAAA GTGAGGATGA AAGGCATGTG AGCTCCATGC CAGCACCCAG ACAGATGAAG 
GAAAACCAGG TGCCCACCAA AGGAACATGG AGGAAAATCA AGGAAAGTGA CATTTCTCCC 
ACAGGCATGG CTTCTCAGAG CGCTTCCTCA GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT 
CTGATCTATC AGATGGCACC TCCTGTCTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG 
GACTGCCCCA TTAACAACCC TAGATCTGGA CGGTCCCCCA CAGGCAACAC CCCCCCAGTG 
ATTGACAGTG TTTCAGAGAA GGGAAGTTCA AGCATTAAAG ATTCAAAAGA CACCCATGGG 
AAACAGAGTG TGGGCAGTGG CAGTCCTGTG CAAACCGTGG GTCTGGAAAC CCGCCTCAAC 
TCCTTTGTTC AGGTAGAGGC CCCAGAACAG AAAGGAACTG AGGCAAAACC AGGACAGAGT 
AACCCAGTCT CTATAGCAGA GACTGCTGAG ACGTGTATAG CAGAGCGTAC CCCTTTCAGT 
TCCAGTAGCT CCAGCAAGCA CAGCTCACCT AGCGGGACTG TTGCTGCCAG AGTGACACCT 
TTTAATTACA ACCCTAGCCC TAGGAAGAGC AGCGCAGACA GCACTTCAGC CCGGCCGTCT 
CAGATCCCTA CGCCAGTGAG CACCAACACG AAGAAGAGAG ATTCGAAGAC TGACAGCACA 
GAATCCAGTG GAGCCCAAAG TCCTAAACGC CATTCCGGGT CTTACCTCGT GACGTCTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525533.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525596.1 Mus musculus adenomatosis polyposis coli (Apc), transcript variant X5, mRNA.

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CloneID	OMu54320
Clone ID Related Accession (Same CDS sequence)	XM_006525533.3 , XM_006525533.2
Accession Version	XM_006525533.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006525533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGT ATTGGCGCAG CTTGCCGGGG TGTCACTGGA GACAGAATGG AGGTGCTGCC 
GGGCTCGAAA ATGGGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGTAGGAAG GCTACCCGGG GCATTTCATC CCAGGAGCTA 
GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC ATTGCAGAGT TATTGCAGGT GGACTGTGAG 
ATGTATGGGC TTACTAATGA CCACTACAGT GTTACTTTAA GACGGTATGC TGGAATGGCT 
TTGACAAACT TGACCTTTGG AGATGTTGCC AACAAGGCTA CGCTGTGTTC TATGAAAGGC 
TGCATGAGAG CACTTGTGGC CCAGTTAAAA TCTGAGAGTG AAGACTTACA GCAGGTTATT 
GCAAGTGTTT TGAGGAATTT GTCTTGGCGA GCAGATGTAA ATAGCAAAAA GACGTTGAGA 
GAAGTTGGAA GTGTGAAAGC ATTGATGGAA TGTGCTTTGG AAGTTAAAAA GGAATCAACC 
CTCAAAAGCG TTTTGAGTGC CTTATGGAAC CTGTCTGCAC ACTGCACTGA GAATAAGGCT 
GACATCTGTG CTGTGGATGG AGCACTGGCA TTTCTGGTTG GCACCCTCAC TTACCGGAGC 
CAGACAAATA CTTTAGCCAT TATTGAAAGT GGAGGTGGGA TATTACGGAA TGTGTCCAGC 
TTGATAGCTA CAAACGAAGA CCACAGGCAA ATCCTAAGAG AGAACAATTG CCTACAAACT 
TTATTACAGC ACTTGAAATC TCACAGCTTG ACAATAGTCA GTAATGCATG TGGAACTTTG 
TGGAATCTCT CAGCAAGAAA TCCTAAAGAC CAGGAAGCCT TGTGGGACAT GGGGGCAGTG 
AGCATGCTCA AGAACCTCAT TCATTCCAAG CACAAAATGA TTGCCATGGG AAGTGCAGCA 
GCTTTAAGGA ATCTCATGGC AAACAGACCT GCAAAGTATA AGGATGCCAA TATCATGTCT 
CCCGGCTCAA GTCTGCCATC CCTTCACGTT AGGAAACAGA AAGCTCTAGA AGCTGAGCTA 
GATGCTCAGC ATTTATCAGA AACCTTCGAC AACATTGACA ACCTAAGTCC CAAGGCCTCT 
CACCGGAGTA AGCAGAGACA CAAGCAGAAT CTTTATGGTG ACTATGCTTT TGACGCCAAT 
CGACATGATG ATAGTAGGTC AGACAATTTC AATACTGGAA ACATGACTGT TCTTTCACCA 
TATTTAAATA CTACGGTATT GCCCAGCTCT TCTTCCTCAA GGGGAAGTTT AGACAGTTCT 
CGTTCTGAGA AAGACAGAAG TTTGGAGAGA GAGCGAGGTA TTGGCCTCAG TGCTTACCAT 
CCAACAACAG AAAATGCAGG AACCTCATCA AAACGAGGTC TGCAGATCAC TACCACTGCA 
GCCCAGATAG CCAAAGTTAT GGAAGAAGTA TCAGCCATTC ATACCTCCCA GGACGACAGA 
AGTTCTGCTT CTACCACCGA GTTCCATTGT GTGGCAGACG ACAGGAGTGC GGCACGAAGA 
AGCTCTGCCT CCCACACACA CTCAAACACA TACAACTTCA CTAAGTCGGA AAATTCAAAT 
AGGACATGCT CTATGCCTTA TGCCAAAGTG GAATATAAAC GATCTTCAAA TGACAGTTTA 
AATAGTGTCA CTAGTAGTGA TGGATATGGT AAAAGAGGCC AAATGAAACC CTCAGTTGAA 
TCCTATTCTG AAGATGATGA AAGTAAATTT TGCAGTTATG GTCAGTATCC AGCTGACCTA 
GCCCATAAGA TACACAGTGC AAATCATATG GATGATAATG ATGGAGAACT GGATACACCA 
ATAAATTACA GTCTTAAATA TTCAGATGAG CAGTTGAACT CAGGAAGGCA GAGTCCCTCA 
CAGAATGAAA GGTGGGCAAG ACCAAAGCAT GTGATAGAAG ATGAAATAAA GCAAAACGAG 
CAAAGACAAG CAAGAAGCCA GAACACCAGT TATCCTGTCT ATTCTGAGAA TACCGATGAC 
AAACACCTCA AATTCCAACC ACATTTTGGA CAACAAGAAT GTGTTTCCCC ATATAGGTCA 
AGGGGAACCA GTGGTTCAGA AACAAATCGA ATGGGTTCTA GTCATGCAAT TAATCAAAAT 
GTAAACCAGT CTCTGTGTCA GGAAGATGAT TATGAAGATG ATAAACCTAC CAACTACAGT 
GAACGTTATT CTGAGGAAGA ACAACATGAA GAAGAAGAAG AGAGACCGAC AAATTATAGC 
ATAAAATATA ATGAAGAGAA ACATCATGTG GATCAGCCTA TTGATTATAG TTTAAAATAT 
GCCACTGACA TTTCTTCCTC ACAAAAACCA TCATTTTCAT TCTCAAAGAA TTCATCAGCA 
CAAAGCACTA AACCTGAACA TCTCTCTCCA AGCAGCGAGA ATACAGCTGT ACCTCCATCT 
AATGCCAAAA GGCAGAATCA GCTGCGTCCA AGTTCAGCAC AAAGAAATGG CCAGACTCAA 
AAAGGCACTA CTTGCAAAGT CCCCTCCATC AACCAAGAAA CAATACAGAC TTACTGCGTA 
GAAGACACCC CAATATGTTT TTCAAGGTGC AGTTCATTAT CATCACTGTC ATCAGCTGAC 
GATGAAATAG GATGTGATCA GACAACACAG GAAGCAGATT CTGCTAATAC TCTGCAGACA 
GCAGAAGTAA AAGAGAATGA TGTAACTCGG TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA 
GCAGTGTCCC AGAATGCTAG AGCCAAACCC AGCCGACTCC AGGCTTCTGG CTTATCTTCA 
GAATCAACCA GGCATAATAA AGCTGTTGAG TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA 
AGTGGTGCTC AGACACCCAA AAGTCCCCCA GAACACTATG TCCAGGAGAC TCCGCTCGTA 
TTCAGCAGGT GTACTTCTGT CAGCTCCCTT GACAGTTTTG AGAGTCGCTC CATTGCCAGC 
TCTGTTCAGA GTGAGCCATG TAGTGGAATG GTGAGTGGCA TCATAAGCCC CAGTGACCTT 
CCAGATAGTC CTGGGCAGAC CATGCCACCA AGCAGAAGCA AAACCCCTCC ACCTCCTCCA 
CAGACAGTGC AGGCCAAGAG AGAGGTGCCA AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA 
GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA 
GACGTGGATA CTTTGTTACA CTTCGCCACA GAAAGTACTC CAGACGGGTT TTCTTGTTCC 
TCCAGCCTAA GTGCTCTGAG CCTGGATGAG CCATTTATAC AGAAAGATGT AGAATTAAGA 
ATCATGCCTC CAGTTCAGGA AAACGACAAT GGGAATGAAA CTGAATCAGA ACAGCCTGAG 
GAATCAAATG AAAACCAGGA TAAAGAGGTA GAAAAGCCTG ACTCTGAAAA AGACTTATTA 
GATGATTCTG ATGACGATGA TATTGAAATA TTAGAAGAAT GTATTATTTC AGCCATGCCA 
ACAAAGTCAT CACGCAAAGC CAAAAAACTA GCCCAGACTG CTTCAAAATT ACCTCCACCT 
GTGGCAAGGA AACCAAGTCA GCTACCTGTG TATAAACTTC TGCCAGCACA GAATAGGCTG 
CAGGCACAAA AACATGTTAG CTTTACACCA GGGGATGATG TGCCCCGGGT GTACTGTGTA 
GAAGGGACAC CTATAAACTT TTCCACAGCA ACGTCTCTAA GTGATCTGAC AATAGAGTCC 
CCTCCAAATG AATTGGCTAC TGGAGATGGG GTCAGAGCGG GTATACAGTC AGGTGAATTT 
GAAAAACGAG ATACCATTCC TACAGAAGGC AGAAGTACAG ATGATGCTCA GCGAGGAAAA 
ATCTCATCTA TAGTTACACC AGACCTGGAT GACAACAAAG CAGAGGAAGG AGATATTCTT 
GCAGAATGTA TCAATTCTGC TATGCCCAAA GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA 
AAGATAATGG ACCAAGTCCA ACAAGCATCC TCGACTTCAT CTGGAGCTAA CAAAAATCAA 
GTAGACACTA AGAAAAAGAA GCCTACTTCA CCAGTAAAGC CCATGCCACA AAATACTGAA 
TATAGAACGC GTGTGAGAAA GAATACAGAC TCAAAAGTTA ATGTAAATAC TGAAGAAACT 
TTCTCAGACA ACAAAGACTC AAAGAAACCA AGCTTACAAA CCAATGCCAA GGCCTTCAAT 
GAAAAGCTAC CTAACAATGA AGACAGAGTG CGGGGGAGCT TCGCCTTGGA CTCACCGCAT 
CACTACACCC CTATTGAGGG GACGCCGTAC TGCTTTTCCC GAAATGACTC CTTGAGTTCT 
CTGGATTTTG ATGATGACGA TGTTGACCTT TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC 
AAAGAAAGCA AGGATTCCGA AGCCAAAGTT ACCTGCCGCC CAGAACCAAA CTCAAGCCAG 
CAGGCAGCTA GTAAGTCACA AGCCAGTATA AAACATCCAG CAAACAGAGC ACAGTCCAAA 
CCAGTGCTGC AGAAACAGCC CACTTTCCCC CAGTCCTCCA AAGACGGACC AGATAGAGGG 
GCAGCAACTG ACGAAAAACT GCAGAATTTT GCTATTGAAA ATACTCCAGT TTGCTTTTCT 
CGAAATTCCT CTCTGAGTTC CCTTAGTGAC ATTGACCAGG AAAACAACAA TAACAAAGAA 
AGTGAACCAA TCAAAGAAGC TGAACCTGCC AACTCACAAG GAGAGCCCAG TAAGCCTCAG 
GCATCCGGGT ATGCTCCCAA GTCCTTCCAC GTCGAAGACA CCCCTGTCTG TTTCTCAAGA 
AACAGCTCTC TCAGTTCTCT TAGCATTGAC TCTGAGGACG ACCTGTTACA GGAGTGTATA 
AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT TCAAGACTCA AGAGTGAGAG CGAAAAGCAG 
AGCCCTAGAA AAGTGGGTGG CATATTAGCT GAAGACCTGA CGCTTGATTT GAAAGATCTA 
CAGAGGCCAG ATTCAGAACA CGCTTTCTCC CCCGACTCAG AAAATTTTGA CTGGAAAGCT 
ATTCAGGAAG GCGCAAACTC CATAGTAAGT AGTTTGCACC AAGCTGCTGC AGCCGCCGCG 
TGCTTATCTA GACAAGCGTC ATCCGACTCA GATTCCATTC TGTCACTAAA GTCCGGCATT 
TCTCTGGGAT CGCCTTTTCA TCTTACACCT GATCAAGAGG AAAAGCCATT CACAAGCAAT 
AAAGGCCCAA GAATTCTCAA ACCTGGAGAG AAAAGCACAT TAGAAGCAAA AAAAATAGAA 
TCTGAAAACA AAGGAATCAA AGGCGGGAAA AAGGTTTATA AAAGCTTGAT TACGGGAAAG 
ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA ATGAAACAAC CCCTCCCGAC AAACATGCCT 
TCAATCTCAA GAGGCAGGAC GATGATTCAC ATCCCAGGGC TTCGGAATAG CTCCTCTAGT 
ACAAGCCCTG TCTCTAAGAA AGGCCCACCC CTCAAGACTC CAGCCTCTAA AAGCCCCAGT 
GAAGGGCCGG GAGCTACCAC TTCTCCTCGA GGAACTAAGC CAGCAGGAAA GTCAGAGCTT 
AGCCCTATCA CCAGGCAAAC TTCCCAAATC AGTGGGTCAA ATAAGGGGTC TTCTAGATCA 
GGATCTAGAG ACTCCACTCC CTCAAGACCT ACACAGCAAC CATTAAGTAG GCCAATGCAG 
TCTCCAGGGC GAAACTCAAT TTCCCCTGGT AGAAATGGAA TAAGCCCTCC TAACAAACTG 
TCTCAGCTGC CCAGAACATC ATCTCCCAGT ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG 
AAAATGTCAT ATACATCCCC AGGTAGACAG CTGAGCCAAC AAAATCTTAC CAAACAAGCA 
AGTTTATCCA AGAATGCCAG CAGTATCCCC AGAAGTGAGT CGGCATCTAA AGGACTGAAT 
CAGATGAGTA ACGGCAATGG GTCAAATAAA AAGGTAGAAC TTTCTAGAAT GTCTTCAACT 
AAATCAAGTG GAAGTGAATC AGACAGATCA GAAAGGCCTG CATTAGTACG CCAGTCTACT 
TTCATCAAAG AAGCCCCAAG CCCAACCCTG AGGAGGAAAC TGGAGGAATC TGCCTCATTT 
GAATCCCTTT CTCCATCTTC TAGACCAGAT TCTCCCACCA GGTCGCAGGC ACAGACCCCA 
GTTTTAAGCC CTTCCCTTCC TGATATGTCT CTGTCCACAC ATCCATCTGT TCAGGCAGGT 
GGGTGGCGAA AGCTCCCGCC TAATCTCAGC CCCACTATCG AGTATAATGA CGGAAGGCCC 
ACAAAACGGC ATGATATTGC ACGCTCCCAT TCTGAAAGTC CTTCCAGACT ACCAATCAAC 
CGGGCGGGAA CCTGGAAGCG TGAACACAGC AAACATTCCT CGTCCCTTCC TCGAGTGAGT 
ACTTGGAGAA GAACTGGAAG CTCATCTTCT ATTCTTTCTG CTTCATCAGA GTCCAGTGAA 
AAAGCAAAAA GTGAGGATGA AAGGCATGTG AGCTCCATGC CAGCACCCAG ACAGATGAAG 
GAAAACCAGG TGCCCACCAA AGGAACATGG AGGAAAATCA AGGAAAGTGA CATTTCTCCC 
ACAGGCATGG CTTCTCAGAG CGCTTCCTCA GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT 
CTGATCTATC AGATGGCACC TCCTGTCTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG 
GACTGCCCCA TTAACAACCC TAGATCTGGA CGGTCCCCCA CAGGCAACAC CCCCCCAGTG 
ATTGACAGTG TTTCAGAGAA GGGAAGTTCA AGCATTAAAG ATTCAAAAGA CACCCATGGG 
AAACAGAGTG TGGGCAGTGG CAGTCCTGTG CAAACCGTGG GTCTGGAAAC CCGCCTCAAC 
TCCTTTGTTC AGGTAGAGGC CCCAGAACAG AAAGGAACTG AGGCAAAACC AGGACAGAGT 
AACCCAGTCT CTATAGCAGA GACTGCTGAG ACGTGTATAG CAGAGCGTAC CCCTTTCAGT 
TCCAGTAGCT CCAGCAAGCA CAGCTCACCT AGCGGGACTG TTGCTGCCAG AGTGACACCT 
TTTAATTACA ACCCTAGCCC TAGGAAGAGC AGCGCAGACA GCACTTCAGC CCGGCCGTCT 
CAGATCCCTA CGCCAGTGAG CACCAACACG AAGAAGAGAG ATTCGAAGAC TGACAGCACA 
GAATCCAGTG GAGCCCAAAG TCCTAAACGC CATTCCGGGT CTTACCTCGT GACGTCTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525596.1
CDS	133..8655
Translation	MWLYWRSLPGCHWRQNGGAAGLENGEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525533.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525533.2

ORF Insert Sequence:

ATGTGGCTGT ATTGGCGCAG CTTGCCGGGG TGTCACTGGA GACAGAATGG AGGTGCTGCC 
GGGCTCGAAA ATGGGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGTAGGAAG GCTACCCGGG GCATTTCATC CCAGGAGCTA 
GGTCAGGGGC TTTCAGGGGG ACTGCAGGCC ATTGCAGAGT TATTGCAGGT GGACTGTGAG 
ATGTATGGGC TTACTAATGA CCACTACAGT GTTACTTTAA GACGGTATGC TGGAATGGCT 
TTGACAAACT TGACCTTTGG AGATGTTGCC AACAAGGCTA CGCTGTGTTC TATGAAAGGC 
TGCATGAGAG CACTTGTGGC CCAGTTAAAA TCTGAGAGTG AAGACTTACA GCAGGTTATT 
GCAAGTGTTT TGAGGAATTT GTCTTGGCGA GCAGATGTAA ATAGCAAAAA GACGTTGAGA 
GAAGTTGGAA GTGTGAAAGC ATTGATGGAA TGTGCTTTGG AAGTTAAAAA GGAATCAACC 
CTCAAAAGCG TTTTGAGTGC CTTATGGAAC CTGTCTGCAC ACTGCACTGA GAATAAGGCT 
GACATCTGTG CTGTGGATGG AGCACTGGCA TTTCTGGTTG GCACCCTCAC TTACCGGAGC 
CAGACAAATA CTTTAGCCAT TATTGAAAGT GGAGGTGGGA TATTACGGAA TGTGTCCAGC 
TTGATAGCTA CAAACGAAGA CCACAGGCAA ATCCTAAGAG AGAACAATTG CCTACAAACT 
TTATTACAGC ACTTGAAATC TCACAGCTTG ACAATAGTCA GTAATGCATG TGGAACTTTG 
TGGAATCTCT CAGCAAGAAA TCCTAAAGAC CAGGAAGCCT TGTGGGACAT GGGGGCAGTG 
AGCATGCTCA AGAACCTCAT TCATTCCAAG CACAAAATGA TTGCCATGGG AAGTGCAGCA 
GCTTTAAGGA ATCTCATGGC AAACAGACCT GCAAAGTATA AGGATGCCAA TATCATGTCT 
CCCGGCTCAA GTCTGCCATC CCTTCACGTT AGGAAACAGA AAGCTCTAGA AGCTGAGCTA 
GATGCTCAGC ATTTATCAGA AACCTTCGAC AACATTGACA ACCTAAGTCC CAAGGCCTCT 
CACCGGAGTA AGCAGAGACA CAAGCAGAAT CTTTATGGTG ACTATGCTTT TGACGCCAAT 
CGACATGATG ATAGTAGGTC AGACAATTTC AATACTGGAA ACATGACTGT TCTTTCACCA 
TATTTAAATA CTACGGTATT GCCCAGCTCT TCTTCCTCAA GGGGAAGTTT AGACAGTTCT 
CGTTCTGAGA AAGACAGAAG TTTGGAGAGA GAGCGAGGTA TTGGCCTCAG TGCTTACCAT 
CCAACAACAG AAAATGCAGG AACCTCATCA AAACGAGGTC TGCAGATCAC TACCACTGCA 
GCCCAGATAG CCAAAGTTAT GGAAGAAGTA TCAGCCATTC ATACCTCCCA GGACGACAGA 
AGTTCTGCTT CTACCACCGA GTTCCATTGT GTGGCAGACG ACAGGAGTGC GGCACGAAGA 
AGCTCTGCCT CCCACACACA CTCAAACACA TACAACTTCA CTAAGTCGGA AAATTCAAAT 
AGGACATGCT CTATGCCTTA TGCCAAAGTG GAATATAAAC GATCTTCAAA TGACAGTTTA 
AATAGTGTCA CTAGTAGTGA TGGATATGGT AAAAGAGGCC AAATGAAACC CTCAGTTGAA 
TCCTATTCTG AAGATGATGA AAGTAAATTT TGCAGTTATG GTCAGTATCC AGCTGACCTA 
GCCCATAAGA TACACAGTGC AAATCATATG GATGATAATG ATGGAGAACT GGATACACCA 
ATAAATTACA GTCTTAAATA TTCAGATGAG CAGTTGAACT CAGGAAGGCA GAGTCCCTCA 
CAGAATGAAA GGTGGGCAAG ACCAAAGCAT GTGATAGAAG ATGAAATAAA GCAAAACGAG 
CAAAGACAAG CAAGAAGCCA GAACACCAGT TATCCTGTCT ATTCTGAGAA TACCGATGAC 
AAACACCTCA AATTCCAACC ACATTTTGGA CAACAAGAAT GTGTTTCCCC ATATAGGTCA 
AGGGGAACCA GTGGTTCAGA AACAAATCGA ATGGGTTCTA GTCATGCAAT TAATCAAAAT 
GTAAACCAGT CTCTGTGTCA GGAAGATGAT TATGAAGATG ATAAACCTAC CAACTACAGT 
GAACGTTATT CTGAGGAAGA ACAACATGAA GAAGAAGAAG AGAGACCGAC AAATTATAGC 
ATAAAATATA ATGAAGAGAA ACATCATGTG GATCAGCCTA TTGATTATAG TTTAAAATAT 
GCCACTGACA TTTCTTCCTC ACAAAAACCA TCATTTTCAT TCTCAAAGAA TTCATCAGCA 
CAAAGCACTA AACCTGAACA TCTCTCTCCA AGCAGCGAGA ATACAGCTGT ACCTCCATCT 
AATGCCAAAA GGCAGAATCA GCTGCGTCCA AGTTCAGCAC AAAGAAATGG CCAGACTCAA 
AAAGGCACTA CTTGCAAAGT CCCCTCCATC AACCAAGAAA CAATACAGAC TTACTGCGTA 
GAAGACACCC CAATATGTTT TTCAAGGTGC AGTTCATTAT CATCACTGTC ATCAGCTGAC 
GATGAAATAG GATGTGATCA GACAACACAG GAAGCAGATT CTGCTAATAC TCTGCAGACA 
GCAGAAGTAA AAGAGAATGA TGTAACTCGG TCAGCTGAAG ATCCTGCAAC TGAAGTTCCA 
GCAGTGTCCC AGAATGCTAG AGCCAAACCC AGCCGACTCC AGGCTTCTGG CTTATCTTCA 
GAATCAACCA GGCATAATAA AGCTGTTGAG TTTTCTTCAG GAGCCAAGTC TCCCTCCAAA 
AGTGGTGCTC AGACACCCAA AAGTCCCCCA GAACACTATG TCCAGGAGAC TCCGCTCGTA 
TTCAGCAGGT GTACTTCTGT CAGCTCCCTT GACAGTTTTG AGAGTCGCTC CATTGCCAGC 
TCTGTTCAGA GTGAGCCATG TAGTGGAATG GTGAGTGGCA TCATAAGCCC CAGTGACCTT 
CCAGATAGTC CTGGGCAGAC CATGCCACCA AGCAGAAGCA AAACCCCTCC ACCTCCTCCA 
CAGACAGTGC AGGCCAAGAG AGAGGTGCCA AAAAGTAAAG TCCCTGCTGC TGAGAAGAGA 
GAGAGTGGGC CTAAGCAGAC TGCTGTAAAT GCTGCCGTGC AGAGGGTGCA GGTCCTTCCA 
GACGTGGATA CTTTGTTACA CTTCGCCACA GAAAGTACTC CAGACGGGTT TTCTTGTTCC 
TCCAGCCTAA GTGCTCTGAG CCTGGATGAG CCATTTATAC AGAAAGATGT AGAATTAAGA 
ATCATGCCTC CAGTTCAGGA AAACGACAAT GGGAATGAAA CTGAATCAGA ACAGCCTGAG 
GAATCAAATG AAAACCAGGA TAAAGAGGTA GAAAAGCCTG ACTCTGAAAA AGACTTATTA 
GATGATTCTG ATGACGATGA TATTGAAATA TTAGAAGAAT GTATTATTTC AGCCATGCCA 
ACAAAGTCAT CACGCAAAGC CAAAAAACTA GCCCAGACTG CTTCAAAATT ACCTCCACCT 
GTGGCAAGGA AACCAAGTCA GCTACCTGTG TATAAACTTC TGCCAGCACA GAATAGGCTG 
CAGGCACAAA AACATGTTAG CTTTACACCA GGGGATGATG TGCCCCGGGT GTACTGTGTA 
GAAGGGACAC CTATAAACTT TTCCACAGCA ACGTCTCTAA GTGATCTGAC AATAGAGTCC 
CCTCCAAATG AATTGGCTAC TGGAGATGGG GTCAGAGCGG GTATACAGTC AGGTGAATTT 
GAAAAACGAG ATACCATTCC TACAGAAGGC AGAAGTACAG ATGATGCTCA GCGAGGAAAA 
ATCTCATCTA TAGTTACACC AGACCTGGAT GACAACAAAG CAGAGGAAGG AGATATTCTT 
GCAGAATGTA TCAATTCTGC TATGCCCAAA GGAAAAAGCC ACAAGCCTTT CCGAGTGAAA 
AAGATAATGG ACCAAGTCCA ACAAGCATCC TCGACTTCAT CTGGAGCTAA CAAAAATCAA 
GTAGACACTA AGAAAAAGAA GCCTACTTCA CCAGTAAAGC CCATGCCACA AAATACTGAA 
TATAGAACGC GTGTGAGAAA GAATACAGAC TCAAAAGTTA ATGTAAATAC TGAAGAAACT 
TTCTCAGACA ACAAAGACTC AAAGAAACCA AGCTTACAAA CCAATGCCAA GGCCTTCAAT 
GAAAAGCTAC CTAACAATGA AGACAGAGTG CGGGGGAGCT TCGCCTTGGA CTCACCGCAT 
CACTACACCC CTATTGAGGG GACGCCGTAC TGCTTTTCCC GAAATGACTC CTTGAGTTCT 
CTGGATTTTG ATGATGACGA TGTTGACCTT TCCAGGGAAA AGGCCGAGTT AAGAAAGGGC 
AAAGAAAGCA AGGATTCCGA AGCCAAAGTT ACCTGCCGCC CAGAACCAAA CTCAAGCCAG 
CAGGCAGCTA GTAAGTCACA AGCCAGTATA AAACATCCAG CAAACAGAGC ACAGTCCAAA 
CCAGTGCTGC AGAAACAGCC CACTTTCCCC CAGTCCTCCA AAGACGGACC AGATAGAGGG 
GCAGCAACTG ACGAAAAACT GCAGAATTTT GCTATTGAAA ATACTCCAGT TTGCTTTTCT 
CGAAATTCCT CTCTGAGTTC CCTTAGTGAC ATTGACCAGG AAAACAACAA TAACAAAGAA 
AGTGAACCAA TCAAAGAAGC TGAACCTGCC AACTCACAAG GAGAGCCCAG TAAGCCTCAG 
GCATCCGGGT ATGCTCCCAA GTCCTTCCAC GTCGAAGACA CCCCTGTCTG TTTCTCAAGA 
AACAGCTCTC TCAGTTCTCT TAGCATTGAC TCTGAGGACG ACCTGTTACA GGAGTGTATA 
AGTTCTGCCA TGCCAAAAAA GAAAAGGCCT TCAAGACTCA AGAGTGAGAG CGAAAAGCAG 
AGCCCTAGAA AAGTGGGTGG CATATTAGCT GAAGACCTGA CGCTTGATTT GAAAGATCTA 
CAGAGGCCAG ATTCAGAACA CGCTTTCTCC CCCGACTCAG AAAATTTTGA CTGGAAAGCT 
ATTCAGGAAG GCGCAAACTC CATAGTAAGT AGTTTGCACC AAGCTGCTGC AGCCGCCGCG 
TGCTTATCTA GACAAGCGTC ATCCGACTCA GATTCCATTC TGTCACTAAA GTCCGGCATT 
TCTCTGGGAT CGCCTTTTCA TCTTACACCT GATCAAGAGG AAAAGCCATT CACAAGCAAT 
AAAGGCCCAA GAATTCTCAA ACCTGGAGAG AAAAGCACAT TAGAAGCAAA AAAAATAGAA 
TCTGAAAACA AAGGAATCAA AGGCGGGAAA AAGGTTTATA AAAGCTTGAT TACGGGAAAG 
ATTCGCTCCA ATTCAGAAAT TTCCAGCCAA ATGAAACAAC CCCTCCCGAC AAACATGCCT 
TCAATCTCAA GAGGCAGGAC GATGATTCAC ATCCCAGGGC TTCGGAATAG CTCCTCTAGT 
ACAAGCCCTG TCTCTAAGAA AGGCCCACCC CTCAAGACTC CAGCCTCTAA AAGCCCCAGT 
GAAGGGCCGG GAGCTACCAC TTCTCCTCGA GGAACTAAGC CAGCAGGAAA GTCAGAGCTT 
AGCCCTATCA CCAGGCAAAC TTCCCAAATC AGTGGGTCAA ATAAGGGGTC TTCTAGATCA 
GGATCTAGAG ACTCCACTCC CTCAAGACCT ACACAGCAAC CATTAAGTAG GCCAATGCAG 
TCTCCAGGGC GAAACTCAAT TTCCCCTGGT AGAAATGGAA TAAGCCCTCC TAACAAACTG 
TCTCAGCTGC CCAGAACATC ATCTCCCAGT ACTGCTTCAA CTAAGTCCTC CGGTTCTGGG 
AAAATGTCAT ATACATCCCC AGGTAGACAG CTGAGCCAAC AAAATCTTAC CAAACAAGCA 
AGTTTATCCA AGAATGCCAG CAGTATCCCC AGAAGTGAGT CGGCATCTAA AGGACTGAAT 
CAGATGAGTA ACGGCAATGG GTCAAATAAA AAGGTAGAAC TTTCTAGAAT GTCTTCAACT 
AAATCAAGTG GAAGTGAATC AGACAGATCA GAAAGGCCTG CATTAGTACG CCAGTCTACT 
TTCATCAAAG AAGCCCCAAG CCCAACCCTG AGGAGGAAAC TGGAGGAATC TGCCTCATTT 
GAATCCCTTT CTCCATCTTC TAGACCAGAT TCTCCCACCA GGTCGCAGGC ACAGACCCCA 
GTTTTAAGCC CTTCCCTTCC TGATATGTCT CTGTCCACAC ATCCATCTGT TCAGGCAGGT 
GGGTGGCGAA AGCTCCCGCC TAATCTCAGC CCCACTATCG AGTATAATGA CGGAAGGCCC 
ACAAAACGGC ATGATATTGC ACGCTCCCAT TCTGAAAGTC CTTCCAGACT ACCAATCAAC 
CGGGCGGGAA CCTGGAAGCG TGAACACAGC AAACATTCCT CGTCCCTTCC TCGAGTGAGT 
ACTTGGAGAA GAACTGGAAG CTCATCTTCT ATTCTTTCTG CTTCATCAGA GTCCAGTGAA 
AAAGCAAAAA GTGAGGATGA AAGGCATGTG AGCTCCATGC CAGCACCCAG ACAGATGAAG 
GAAAACCAGG TGCCCACCAA AGGAACATGG AGGAAAATCA AGGAAAGTGA CATTTCTCCC 
ACAGGCATGG CTTCTCAGAG CGCTTCCTCA GGTGCTGCCA GTGGTGCTGA ATCCAAGCCT 
CTGATCTATC AGATGGCACC TCCTGTCTCT AAAACAGAGG ATGTTTGGGT GAGAATTGAG 
GACTGCCCCA TTAACAACCC TAGATCTGGA CGGTCCCCCA CAGGCAACAC CCCCCCAGTG 
ATTGACAGTG TTTCAGAGAA GGGAAGTTCA AGCATTAAAG ATTCAAAAGA CACCCATGGG 
AAACAGAGTG TGGGCAGTGG CAGTCCTGTG CAAACCGTGG GTCTGGAAAC CCGCCTCAAC 
TCCTTTGTTC AGGTAGAGGC CCCAGAACAG AAAGGAACTG AGGCAAAACC AGGACAGAGT 
AACCCAGTCT CTATAGCAGA GACTGCTGAG ACGTGTATAG CAGAGCGTAC CCCTTTCAGT 
TCCAGTAGCT CCAGCAAGCA CAGCTCACCT AGCGGGACTG TTGCTGCCAG AGTGACACCT 
TTTAATTACA ACCCTAGCCC TAGGAAGAGC AGCGCAGACA GCACTTCAGC CCGGCCGTCT 
CAGATCCCTA CGCCAGTGAG CACCAACACG AAGAAGAGAG ATTCGAAGAC TGACAGCACA 
GAATCCAGTG GAGCCCAAAG TCCTAAACGC CATTCCGGGT CTTACCTCGT GACGTCTGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525534.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525597.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X6, mRNA.

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CloneID	OMu54321
Clone ID Related Accession (Same CDS sequence)	XM_006525534.4 , XM_006525534.3
Accession Version	XM_006525534.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525534.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGTCCAG CTACACGTGT GGATCACGAA 
ACAGCCAGTG TTTTGAGTTC TAGCGGCACG CACTCTGCTC CTCGAAGGTT GACAAGTCAT 
CTGGGGACAA AGGTGGAAAT GGTGTATTCC TTGTTGTCAA TGCTTGGTAC TCATGATAAG 
GACGATATGT CACGAACTTT GCTAGCTATG TCCAGCTCCC AAGACAGCTG TATATCCATG 
CGGCAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT 
GTATTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC 
CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT 
CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAC 
GAACAAGGCA TGGACCAGGA CAAAAACCCA ATGCCAGCTC CTGTTGAGCA TCAGATCTGT 
CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA TTTGATGAAG AGCATAGGCA TGCAATGAAT 
GAACTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGTCA GGGGCTTTCA 
GGGGGACTGC AGGCCATTGC AGAGTTATTG CAGGTGGACT GTGAGATGTA TGGGCTTACT 
AATGACCACT ACAGTGTTAC TTTAAGACGG TATGCTGGAA TGGCTTTGAC AAACTTGACC 
TTTGGAGATG TTGCCAACAA GGCTACGCTG TGTTCTATGA AAGGCTGCAT GAGAGCACTT 
GTGGCCCAGT TAAAATCTGA GAGTGAAGAC TTACAGCAGG TTATTGCAAG TGTTTTGAGG 
AATTTGTCTT GGCGAGCAGA TGTAAATAGC AAAAAGACGT TGAGAGAAGT TGGAAGTGTG 
AAAGCATTGA TGGAATGTGC TTTGGAAGTT AAAAAGGAAT CAACCCTCAA AAGCGTTTTG 
AGTGCCTTAT GGAACCTGTC TGCACACTGC ACTGAGAATA AGGCTGACAT CTGTGCTGTG 
GATGGAGCAC TGGCATTTCT GGTTGGCACC CTCACTTACC GGAGCCAGAC AAATACTTTA 
GCCATTATTG AAAGTGGAGG TGGGATATTA CGGAATGTGT CCAGCTTGAT AGCTACAAAC 
GAAGACCACA GGCAAATCCT AAGAGAGAAC AATTGCCTAC AAACTTTATT ACAGCACTTG 
AAATCTCACA GCTTGACAAT AGTCAGTAAT GCATGTGGAA CTTTGTGGAA TCTCTCAGCA 
AGAAATCCTA AAGACCAGGA AGCCTTGTGG GACATGGGGG CAGTGAGCAT GCTCAAGAAC 
CTCATTCATT CCAAGCACAA AATGATTGCC ATGGGAAGTG CAGCAGCTTT AAGGAATCTC 
ATGGCAAACA GACCTGCAAA GTATAAGGAT GCCAATATCA TGTCTCCCGG CTCAAGTCTG 
CCATCCCTTC ACGTTAGGAA ACAGAAAGCT CTAGAAGCTG AGCTAGATGC TCAGCATTTA 
TCAGAAACCT TCGACAACAT TGACAACCTA AGTCCCAAGG CCTCTCACCG GAGTAAGCAG 
AGACACAAGC AGAATCTTTA TGGTGACTAT GCTTTTGACG CCAATCGACA TGATGATAGT 
AGGTCAGACA ATTTCAATAC TGGAAACATG ACTGTTCTTT CACCATATTT AAATACTACG 
GTATTGCCCA GCTCTTCTTC CTCAAGGGGA AGTTTAGACA GTTCTCGTTC TGAGAAAGAC 
AGAAGTTTGG AGAGAGAGCG AGGTATTGGC CTCAGTGCTT ACCATCCAAC AACAGAAAAT 
GCAGGAACCT CATCAAAACG AGGTCTGCAG ATCACTACCA CTGCAGCCCA GATAGCCAAA 
GTTATGGAAG AAGTATCAGC CATTCATACC TCCCAGGACG ACAGAAGTTC TGCTTCTACC 
ACCGAGTTCC ATTGTGTGGC AGACGACAGG AGTGCGGCAC GAAGAAGCTC TGCCTCCCAC 
ACACACTCAA ACACATACAA CTTCACTAAG TCGGAAAATT CAAATAGGAC ATGCTCTATG 
CCTTATGCCA AAGTGGAATA TAAACGATCT TCAAATGACA GTTTAAATAG TGTCACTAGT 
AGTGATGGAT ATGGTAAAAG AGGCCAAATG AAACCCTCAG TTGAATCCTA TTCTGAAGAT 
GATGAAAGTA AATTTTGCAG TTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAGATACAC 
AGTGCAAATC ATATGGATGA TAATGATGGA GAACTGGATA CACCAATAAA TTACAGTCTT 
AAATATTCAG ATGAGCAGTT GAACTCAGGA AGGCAGAGTC CCTCACAGAA TGAAAGGTGG 
GCAAGACCAA AGCATGTGAT AGAAGATGAA ATAAAGCAAA ACGAGCAAAG ACAAGCAAGA 
AGCCAGAACA CCAGTTATCC TGTCTATTCT GAGAATACCG ATGACAAACA CCTCAAATTC 
CAACCACATT TTGGACAACA AGAATGTGTT TCCCCATATA GGTCAAGGGG AACCAGTGGT 
TCAGAAACAA ATCGAATGGG TTCTAGTCAT GCAATTAATC AAAATGTAAA CCAGTCTCTG 
TGTCAGGAAG ATGATTATGA AGATGATAAA CCTACCAACT ACAGTGAACG TTATTCTGAG 
GAAGAACAAC ATGAAGAAGA AGAAGAGAGA CCGACAAATT ATAGCATAAA ATATAATGAA 
GAGAAACATC ATGTGGATCA GCCTATTGAT TATAGTTTAA AATATGCCAC TGACATTTCT 
TCCTCACAAA AACCATCATT TTCATTCTCA AAGAATTCAT CAGCACAAAG CACTAAACCT 
GAACATCTCT CTCCAAGCAG CGAGAATACA GCTGTACCTC CATCTAATGC CAAAAGGCAG 
AATCAGCTGC GTCCAAGTTC AGCACAAAGA AATGGCCAGA CTCAAAAAGG CACTACTTGC 
AAAGTCCCCT CCATCAACCA AGAAACAATA CAGACTTACT GCGTAGAAGA CACCCCAATA 
TGTTTTTCAA GGTGCAGTTC ATTATCATCA CTGTCATCAG CTGACGATGA AATAGGATGT 
GATCAGACAA CACAGGAAGC AGATTCTGCT AATACTCTGC AGACAGCAGA AGTAAAAGAG 
AATGATGTAA CTCGGTCAGC TGAAGATCCT GCAACTGAAG TTCCAGCAGT GTCCCAGAAT 
GCTAGAGCCA AACCCAGCCG ACTCCAGGCT TCTGGCTTAT CTTCAGAATC AACCAGGCAT 
AATAAAGCTG TTGAGTTTTC TTCAGGAGCC AAGTCTCCCT CCAAAAGTGG TGCTCAGACA 
CCCAAAAGTC CCCCAGAACA CTATGTCCAG GAGACTCCGC TCGTATTCAG CAGGTGTACT 
TCTGTCAGCT CCCTTGACAG TTTTGAGAGT CGCTCCATTG CCAGCTCTGT TCAGAGTGAG 
CCATGTAGTG GAATGGTGAG TGGCATCATA AGCCCCAGTG ACCTTCCAGA TAGTCCTGGG 
CAGACCATGC CACCAAGCAG AAGCAAAACC CCTCCACCTC CTCCACAGAC AGTGCAGGCC 
AAGAGAGAGG TGCCAAAAAG TAAAGTCCCT GCTGCTGAGA AGAGAGAGAG TGGGCCTAAG 
CAGACTGCTG TAAATGCTGC CGTGCAGAGG GTGCAGGTCC TTCCAGACGT GGATACTTTG 
TTACACTTCG CCACAGAAAG TACTCCAGAC GGGTTTTCTT GTTCCTCCAG CCTAAGTGCT 
CTGAGCCTGG ATGAGCCATT TATACAGAAA GATGTAGAAT TAAGAATCAT GCCTCCAGTT 
CAGGAAAACG ACAATGGGAA TGAAACTGAA TCAGAACAGC CTGAGGAATC AAATGAAAAC 
CAGGATAAAG AGGTAGAAAA GCCTGACTCT GAAAAAGACT TATTAGATGA TTCTGATGAC 
GATGATATTG AAATATTAGA AGAATGTATT ATTTCAGCCA TGCCAACAAA GTCATCACGC 
AAAGCCAAAA AACTAGCCCA GACTGCTTCA AAATTACCTC CACCTGTGGC AAGGAAACCA 
AGTCAGCTAC CTGTGTATAA ACTTCTGCCA GCACAGAATA GGCTGCAGGC ACAAAAACAT 
GTTAGCTTTA CACCAGGGGA TGATGTGCCC CGGGTGTACT GTGTAGAAGG GACACCTATA 
AACTTTTCCA CAGCAACGTC TCTAAGTGAT CTGACAATAG AGTCCCCTCC AAATGAATTG 
GCTACTGGAG ATGGGGTCAG AGCGGGTATA CAGTCAGGTG AATTTGAAAA ACGAGATACC 
ATTCCTACAG AAGGCAGAAG TACAGATGAT GCTCAGCGAG GAAAAATCTC ATCTATAGTT 
ACACCAGACC TGGATGACAA CAAAGCAGAG GAAGGAGATA TTCTTGCAGA ATGTATCAAT 
TCTGCTATGC CCAAAGGAAA AAGCCACAAG CCTTTCCGAG TGAAAAAGAT AATGGACCAA 
GTCCAACAAG CATCCTCGAC TTCATCTGGA GCTAACAAAA ATCAAGTAGA CACTAAGAAA 
AAGAAGCCTA CTTCACCAGT AAAGCCCATG CCACAAAATA CTGAATATAG AACGCGTGTG 
AGAAAGAATA CAGACTCAAA AGTTAATGTA AATACTGAAG AAACTTTCTC AGACAACAAA 
GACTCAAAGA AACCAAGCTT ACAAACCAAT GCCAAGGCCT TCAATGAAAA GCTACCTAAC 
AATGAAGACA GAGTGCGGGG GAGCTTCGCC TTGGACTCAC CGCATCACTA CACCCCTATT 
GAGGGGACGC CGTACTGCTT TTCCCGAAAT GACTCCTTGA GTTCTCTGGA TTTTGATGAT 
GACGATGTTG ACCTTTCCAG GGAAAAGGCC GAGTTAAGAA AGGGCAAAGA AAGCAAGGAT 
TCCGAAGCCA AAGTTACCTG CCGCCCAGAA CCAAACTCAA GCCAGCAGGC AGCTAGTAAG 
TCACAAGCCA GTATAAAACA TCCAGCAAAC AGAGCACAGT CCAAACCAGT GCTGCAGAAA 
CAGCCCACTT TCCCCCAGTC CTCCAAAGAC GGACCAGATA GAGGGGCAGC AACTGACGAA 
AAACTGCAGA ATTTTGCTAT TGAAAATACT CCAGTTTGCT TTTCTCGAAA TTCCTCTCTG 
AGTTCCCTTA GTGACATTGA CCAGGAAAAC AACAATAACA AAGAAAGTGA ACCAATCAAA 
GAAGCTGAAC CTGCCAACTC ACAAGGAGAG CCCAGTAAGC CTCAGGCATC CGGGTATGCT 
CCCAAGTCCT TCCACGTCGA AGACACCCCT GTCTGTTTCT CAAGAAACAG CTCTCTCAGT 
TCTCTTAGCA TTGACTCTGA GGACGACCTG TTACAGGAGT GTATAAGTTC TGCCATGCCA 
AAAAAGAAAA GGCCTTCAAG ACTCAAGAGT GAGAGCGAAA AGCAGAGCCC TAGAAAAGTG 
GGTGGCATAT TAGCTGAAGA CCTGACGCTT GATTTGAAAG ATCTACAGAG GCCAGATTCA 
GAACACGCTT TCTCCCCCGA CTCAGAAAAT TTTGACTGGA AAGCTATTCA GGAAGGCGCA 
AACTCCATAG TAAGTAGTTT GCACCAAGCT GCTGCAGCCG CCGCGTGCTT ATCTAGACAA 
GCGTCATCCG ACTCAGATTC CATTCTGTCA CTAAAGTCCG GCATTTCTCT GGGATCGCCT 
TTTCATCTTA CACCTGATCA AGAGGAAAAG CCATTCACAA GCAATAAAGG CCCAAGAATT 
CTCAAACCTG GAGAGAAAAG CACATTAGAA GCAAAAAAAA TAGAATCTGA AAACAAAGGA 
ATCAAAGGCG GGAAAAAGGT TTATAAAAGC TTGATTACGG GAAAGATTCG CTCCAATTCA 
GAAATTTCCA GCCAAATGAA ACAACCCCTC CCGACAAACA TGCCTTCAAT CTCAAGAGGC 
AGGACGATGA TTCACATCCC AGGGCTTCGG AATAGCTCCT CTAGTACAAG CCCTGTCTCT 
AAGAAAGGCC CACCCCTCAA GACTCCAGCC TCTAAAAGCC CCAGTGAAGG GCCGGGAGCT 
ACCACTTCTC CTCGAGGAAC TAAGCCAGCA GGAAAGTCAG AGCTTAGCCC TATCACCAGG 
CAAACTTCCC AAATCAGTGG GTCAAATAAG GGGTCTTCTA GATCAGGATC TAGAGACTCC 
ACTCCCTCAA GACCTACACA GCAACCATTA AGTAGGCCAA TGCAGTCTCC AGGGCGAAAC 
TCAATTTCCC CTGGTAGAAA TGGAATAAGC CCTCCTAACA AACTGTCTCA GCTGCCCAGA 
ACATCATCTC CCAGTACTGC TTCAACTAAG TCCTCCGGTT CTGGGAAAAT GTCATATACA 
TCCCCAGGTA GACAGCTGAG CCAACAAAAT CTTACCAAAC AAGCAAGTTT ATCCAAGAAT 
GCCAGCAGTA TCCCCAGAAG TGAGTCGGCA TCTAAAGGAC TGAATCAGAT GAGTAACGGC 
AATGGGTCAA ATAAAAAGGT AGAACTTTCT AGAATGTCTT CAACTAAATC AAGTGGAAGT 
GAATCAGACA GATCAGAAAG GCCTGCATTA GTACGCCAGT CTACTTTCAT CAAAGAAGCC 
CCAAGCCCAA CCCTGAGGAG GAAACTGGAG GAATCTGCCT CATTTGAATC CCTTTCTCCA 
TCTTCTAGAC CAGATTCTCC CACCAGGTCG CAGGCACAGA CCCCAGTTTT AAGCCCTTCC 
CTTCCTGATA TGTCTCTGTC CACACATCCA TCTGTTCAGG CAGGTGGGTG GCGAAAGCTC 
CCGCCTAATC TCAGCCCCAC TATCGAGTAT AATGACGGAA GGCCCACAAA ACGGCATGAT 
ATTGCACGCT CCCATTCTGA AAGTCCTTCC AGACTACCAA TCAACCGGGC GGGAACCTGG 
AAGCGTGAAC ACAGCAAACA TTCCTCGTCC CTTCCTCGAG TGAGTACTTG GAGAAGAACT 
GGAAGCTCAT CTTCTATTCT TTCTGCTTCA TCAGAGTCCA GTGAAAAAGC AAAAAGTGAG 
GATGAAAGGC ATGTGAGCTC CATGCCAGCA CCCAGACAGA TGAAGGAAAA CCAGGTGCCC 
ACCAAAGGAA CATGGAGGAA AATCAAGGAA AGTGACATTT CTCCCACAGG CATGGCTTCT 
CAGAGCGCTT CCTCAGGTGC TGCCAGTGGT GCTGAATCCA AGCCTCTGAT CTATCAGATG 
GCACCTCCTG TCTCTAAAAC AGAGGATGTT TGGGTGAGAA TTGAGGACTG CCCCATTAAC 
AACCCTAGAT CTGGACGGTC CCCCACAGGC AACACCCCCC CAGTGATTGA CAGTGTTTCA 
GAGAAGGGAA GTTCAAGCAT TAAAGATTCA AAAGACACCC ATGGGAAACA GAGTGTGGGC 
AGTGGCAGTC CTGTGCAAAC CGTGGGTCTG GAAACCCGCC TCAACTCCTT TGTTCAGGTA 
GAGGCCCCAG AACAGAAAGG AACTGAGGCA AAACCAGGAC AGAGTAACCC AGTCTCTATA 
GCAGAGACTG CTGAGACGTG TATAGCAGAG CGTACCCCTT TCAGTTCCAG TAGCTCCAGC 
AAGCACAGCT CACCTAGCGG GACTGTTGCT GCCAGAGTGA CACCTTTTAA TTACAACCCT 
AGCCCTAGGA AGAGCAGCGC AGACAGCACT TCAGCCCGGC CGTCTCAGAT CCCTACGCCA 
GTGAGCACCA ACACGAAGAA GAGAGATTCG AAGACTGACA GCACAGAATC CAGTGGAGCC 
CAAAGTCCTA AACGCCATTC CGGGTCTTAC CTCGTGACGT CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525597.1
CDS	816..9323
Translation	MYAFLRSGPVAALPASVPPVTLGARSSGGGRGRVPGEGRRPGGARACGRGASVWQEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAESPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525534.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525534.4

ORF Insert Sequence:

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGTCCAG CTACACGTGT GGATCACGAA 
ACAGCCAGTG TTTTGAGTTC TAGCGGCACG CACTCTGCTC CTCGAAGGTT GACAAGTCAT 
CTGGGGACAA AGGTGGAAAT GGTGTATTCC TTGTTGTCAA TGCTTGGTAC TCATGATAAG 
GACGATATGT CACGAACTTT GCTAGCTATG TCCAGCTCCC AAGACAGCTG TATATCCATG 
CGGCAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT 
GTATTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC 
CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT 
CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAC 
GAACAAGGCA TGGACCAGGA CAAAAACCCA ATGCCAGCTC CTGTTGAGCA TCAGATCTGT 
CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA TTTGATGAAG AGCATAGGCA TGCAATGAAT 
GAACTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGTCA GGGGCTTTCA 
GGGGGACTGC AGGCCATTGC AGAGTTATTG CAGGTGGACT GTGAGATGTA TGGGCTTACT 
AATGACCACT ACAGTGTTAC TTTAAGACGG TATGCTGGAA TGGCTTTGAC AAACTTGACC 
TTTGGAGATG TTGCCAACAA GGCTACGCTG TGTTCTATGA AAGGCTGCAT GAGAGCACTT 
GTGGCCCAGT TAAAATCTGA GAGTGAAGAC TTACAGCAGG TTATTGCAAG TGTTTTGAGG 
AATTTGTCTT GGCGAGCAGA TGTAAATAGC AAAAAGACGT TGAGAGAAGT TGGAAGTGTG 
AAAGCATTGA TGGAATGTGC TTTGGAAGTT AAAAAGGAAT CAACCCTCAA AAGCGTTTTG 
AGTGCCTTAT GGAACCTGTC TGCACACTGC ACTGAGAATA AGGCTGACAT CTGTGCTGTG 
GATGGAGCAC TGGCATTTCT GGTTGGCACC CTCACTTACC GGAGCCAGAC AAATACTTTA 
GCCATTATTG AAAGTGGAGG TGGGATATTA CGGAATGTGT CCAGCTTGAT AGCTACAAAC 
GAAGACCACA GGCAAATCCT AAGAGAGAAC AATTGCCTAC AAACTTTATT ACAGCACTTG 
AAATCTCACA GCTTGACAAT AGTCAGTAAT GCATGTGGAA CTTTGTGGAA TCTCTCAGCA 
AGAAATCCTA AAGACCAGGA AGCCTTGTGG GACATGGGGG CAGTGAGCAT GCTCAAGAAC 
CTCATTCATT CCAAGCACAA AATGATTGCC ATGGGAAGTG CAGCAGCTTT AAGGAATCTC 
ATGGCAAACA GACCTGCAAA GTATAAGGAT GCCAATATCA TGTCTCCCGG CTCAAGTCTG 
CCATCCCTTC ACGTTAGGAA ACAGAAAGCT CTAGAAGCTG AGCTAGATGC TCAGCATTTA 
TCAGAAACCT TCGACAACAT TGACAACCTA AGTCCCAAGG CCTCTCACCG GAGTAAGCAG 
AGACACAAGC AGAATCTTTA TGGTGACTAT GCTTTTGACG CCAATCGACA TGATGATAGT 
AGGTCAGACA ATTTCAATAC TGGAAACATG ACTGTTCTTT CACCATATTT AAATACTACG 
GTATTGCCCA GCTCTTCTTC CTCAAGGGGA AGTTTAGACA GTTCTCGTTC TGAGAAAGAC 
AGAAGTTTGG AGAGAGAGCG AGGTATTGGC CTCAGTGCTT ACCATCCAAC AACAGAAAAT 
GCAGGAACCT CATCAAAACG AGGTCTGCAG ATCACTACCA CTGCAGCCCA GATAGCCAAA 
GTTATGGAAG AAGTATCAGC CATTCATACC TCCCAGGACG ACAGAAGTTC TGCTTCTACC 
ACCGAGTTCC ATTGTGTGGC AGACGACAGG AGTGCGGCAC GAAGAAGCTC TGCCTCCCAC 
ACACACTCAA ACACATACAA CTTCACTAAG TCGGAAAATT CAAATAGGAC ATGCTCTATG 
CCTTATGCCA AAGTGGAATA TAAACGATCT TCAAATGACA GTTTAAATAG TGTCACTAGT 
AGTGATGGAT ATGGTAAAAG AGGCCAAATG AAACCCTCAG TTGAATCCTA TTCTGAAGAT 
GATGAAAGTA AATTTTGCAG TTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAGATACAC 
AGTGCAAATC ATATGGATGA TAATGATGGA GAACTGGATA CACCAATAAA TTACAGTCTT 
AAATATTCAG ATGAGCAGTT GAACTCAGGA AGGCAGAGTC CCTCACAGAA TGAAAGGTGG 
GCAAGACCAA AGCATGTGAT AGAAGATGAA ATAAAGCAAA ACGAGCAAAG ACAAGCAAGA 
AGCCAGAACA CCAGTTATCC TGTCTATTCT GAGAATACCG ATGACAAACA CCTCAAATTC 
CAACCACATT TTGGACAACA AGAATGTGTT TCCCCATATA GGTCAAGGGG AACCAGTGGT 
TCAGAAACAA ATCGAATGGG TTCTAGTCAT GCAATTAATC AAAATGTAAA CCAGTCTCTG 
TGTCAGGAAG ATGATTATGA AGATGATAAA CCTACCAACT ACAGTGAACG TTATTCTGAG 
GAAGAACAAC ATGAAGAAGA AGAAGAGAGA CCGACAAATT ATAGCATAAA ATATAATGAA 
GAGAAACATC ATGTGGATCA GCCTATTGAT TATAGTTTAA AATATGCCAC TGACATTTCT 
TCCTCACAAA AACCATCATT TTCATTCTCA AAGAATTCAT CAGCACAAAG CACTAAACCT 
GAACATCTCT CTCCAAGCAG CGAGAATACA GCTGTACCTC CATCTAATGC CAAAAGGCAG 
AATCAGCTGC GTCCAAGTTC AGCACAAAGA AATGGCCAGA CTCAAAAAGG CACTACTTGC 
AAAGTCCCCT CCATCAACCA AGAAACAATA CAGACTTACT GCGTAGAAGA CACCCCAATA 
TGTTTTTCAA GGTGCAGTTC ATTATCATCA CTGTCATCAG CTGACGATGA AATAGGATGT 
GATCAGACAA CACAGGAAGC AGATTCTGCT AATACTCTGC AGACAGCAGA AGTAAAAGAG 
AATGATGTAA CTCGGTCAGC TGAAGATCCT GCAACTGAAG TTCCAGCAGT GTCCCAGAAT 
GCTAGAGCCA AACCCAGCCG ACTCCAGGCT TCTGGCTTAT CTTCAGAATC AACCAGGCAT 
AATAAAGCTG TTGAGTTTTC TTCAGGAGCC AAGTCTCCCT CCAAAAGTGG TGCTCAGACA 
CCCAAAAGTC CCCCAGAACA CTATGTCCAG GAGACTCCGC TCGTATTCAG CAGGTGTACT 
TCTGTCAGCT CCCTTGACAG TTTTGAGAGT CGCTCCATTG CCAGCTCTGT TCAGAGTGAG 
CCATGTAGTG GAATGGTGAG TGGCATCATA AGCCCCAGTG ACCTTCCAGA TAGTCCTGGG 
CAGACCATGC CACCAAGCAG AAGCAAAACC CCTCCACCTC CTCCACAGAC AGTGCAGGCC 
AAGAGAGAGG TGCCAAAAAG TAAAGTCCCT GCTGCTGAGA AGAGAGAGAG TGGGCCTAAG 
CAGACTGCTG TAAATGCTGC CGTGCAGAGG GTGCAGGTCC TTCCAGACGT GGATACTTTG 
TTACACTTCG CCACAGAAAG TACTCCAGAC GGGTTTTCTT GTTCCTCCAG CCTAAGTGCT 
CTGAGCCTGG ATGAGCCATT TATACAGAAA GATGTAGAAT TAAGAATCAT GCCTCCAGTT 
CAGGAAAACG ACAATGGGAA TGAAACTGAA TCAGAACAGC CTGAGGAATC AAATGAAAAC 
CAGGATAAAG AGGTAGAAAA GCCTGACTCT GAAAAAGACT TATTAGATGA TTCTGATGAC 
GATGATATTG AAATATTAGA AGAATGTATT ATTTCAGCCA TGCCAACAAA GTCATCACGC 
AAAGCCAAAA AACTAGCCCA GACTGCTTCA AAATTACCTC CACCTGTGGC AAGGAAACCA 
AGTCAGCTAC CTGTGTATAA ACTTCTGCCA GCACAGAATA GGCTGCAGGC ACAAAAACAT 
GTTAGCTTTA CACCAGGGGA TGATGTGCCC CGGGTGTACT GTGTAGAAGG GACACCTATA 
AACTTTTCCA CAGCAACGTC TCTAAGTGAT CTGACAATAG AGTCCCCTCC AAATGAATTG 
GCTACTGGAG ATGGGGTCAG AGCGGGTATA CAGTCAGGTG AATTTGAAAA ACGAGATACC 
ATTCCTACAG AAGGCAGAAG TACAGATGAT GCTCAGCGAG GAAAAATCTC ATCTATAGTT 
ACACCAGACC TGGATGACAA CAAAGCAGAG GAAGGAGATA TTCTTGCAGA ATGTATCAAT 
TCTGCTATGC CCAAAGGAAA AAGCCACAAG CCTTTCCGAG TGAAAAAGAT AATGGACCAA 
GTCCAACAAG CATCCTCGAC TTCATCTGGA GCTAACAAAA ATCAAGTAGA CACTAAGAAA 
AAGAAGCCTA CTTCACCAGT AAAGCCCATG CCACAAAATA CTGAATATAG AACGCGTGTG 
AGAAAGAATA CAGACTCAAA AGTTAATGTA AATACTGAAG AAACTTTCTC AGACAACAAA 
GACTCAAAGA AACCAAGCTT ACAAACCAAT GCCAAGGCCT TCAATGAAAA GCTACCTAAC 
AATGAAGACA GAGTGCGGGG GAGCTTCGCC TTGGACTCAC CGCATCACTA CACCCCTATT 
GAGGGGACGC CGTACTGCTT TTCCCGAAAT GACTCCTTGA GTTCTCTGGA TTTTGATGAT 
GACGATGTTG ACCTTTCCAG GGAAAAGGCC GAGTTAAGAA AGGGCAAAGA AAGCAAGGAT 
TCCGAAGCCA AAGTTACCTG CCGCCCAGAA CCAAACTCAA GCCAGCAGGC AGCTAGTAAG 
TCACAAGCCA GTATAAAACA TCCAGCAAAC AGAGCACAGT CCAAACCAGT GCTGCAGAAA 
CAGCCCACTT TCCCCCAGTC CTCCAAAGAC GGACCAGATA GAGGGGCAGC AACTGACGAA 
AAACTGCAGA ATTTTGCTAT TGAAAATACT CCAGTTTGCT TTTCTCGAAA TTCCTCTCTG 
AGTTCCCTTA GTGACATTGA CCAGGAAAAC AACAATAACA AAGAAAGTGA ACCAATCAAA 
GAAGCTGAAC CTGCCAACTC ACAAGGAGAG CCCAGTAAGC CTCAGGCATC CGGGTATGCT 
CCCAAGTCCT TCCACGTCGA AGACACCCCT GTCTGTTTCT CAAGAAACAG CTCTCTCAGT 
TCTCTTAGCA TTGACTCTGA GGACGACCTG TTACAGGAGT GTATAAGTTC TGCCATGCCA 
AAAAAGAAAA GGCCTTCAAG ACTCAAGAGT GAGAGCGAAA AGCAGAGCCC TAGAAAAGTG 
GGTGGCATAT TAGCTGAAGA CCTGACGCTT GATTTGAAAG ATCTACAGAG GCCAGATTCA 
GAACACGCTT TCTCCCCCGA CTCAGAAAAT TTTGACTGGA AAGCTATTCA GGAAGGCGCA 
AACTCCATAG TAAGTAGTTT GCACCAAGCT GCTGCAGCCG CCGCGTGCTT ATCTAGACAA 
GCGTCATCCG ACTCAGATTC CATTCTGTCA CTAAAGTCCG GCATTTCTCT GGGATCGCCT 
TTTCATCTTA CACCTGATCA AGAGGAAAAG CCATTCACAA GCAATAAAGG CCCAAGAATT 
CTCAAACCTG GAGAGAAAAG CACATTAGAA GCAAAAAAAA TAGAATCTGA AAACAAAGGA 
ATCAAAGGCG GGAAAAAGGT TTATAAAAGC TTGATTACGG GAAAGATTCG CTCCAATTCA 
GAAATTTCCA GCCAAATGAA ACAACCCCTC CCGACAAACA TGCCTTCAAT CTCAAGAGGC 
AGGACGATGA TTCACATCCC AGGGCTTCGG AATAGCTCCT CTAGTACAAG CCCTGTCTCT 
AAGAAAGGCC CACCCCTCAA GACTCCAGCC TCTAAAAGCC CCAGTGAAGG GCCGGGAGCT 
ACCACTTCTC CTCGAGGAAC TAAGCCAGCA GGAAAGTCAG AGCTTAGCCC TATCACCAGG 
CAAACTTCCC AAATCAGTGG GTCAAATAAG GGGTCTTCTA GATCAGGATC TAGAGACTCC 
ACTCCCTCAA GACCTACACA GCAACCATTA AGTAGGCCAA TGCAGTCTCC AGGGCGAAAC 
TCAATTTCCC CTGGTAGAAA TGGAATAAGC CCTCCTAACA AACTGTCTCA GCTGCCCAGA 
ACATCATCTC CCAGTACTGC TTCAACTAAG TCCTCCGGTT CTGGGAAAAT GTCATATACA 
TCCCCAGGTA GACAGCTGAG CCAACAAAAT CTTACCAAAC AAGCAAGTTT ATCCAAGAAT 
GCCAGCAGTA TCCCCAGAAG TGAGTCGGCA TCTAAAGGAC TGAATCAGAT GAGTAACGGC 
AATGGGTCAA ATAAAAAGGT AGAACTTTCT AGAATGTCTT CAACTAAATC AAGTGGAAGT 
GAATCAGACA GATCAGAAAG GCCTGCATTA GTACGCCAGT CTACTTTCAT CAAAGAAGCC 
CCAAGCCCAA CCCTGAGGAG GAAACTGGAG GAATCTGCCT CATTTGAATC CCTTTCTCCA 
TCTTCTAGAC CAGATTCTCC CACCAGGTCG CAGGCACAGA CCCCAGTTTT AAGCCCTTCC 
CTTCCTGATA TGTCTCTGTC CACACATCCA TCTGTTCAGG CAGGTGGGTG GCGAAAGCTC 
CCGCCTAATC TCAGCCCCAC TATCGAGTAT AATGACGGAA GGCCCACAAA ACGGCATGAT 
ATTGCACGCT CCCATTCTGA AAGTCCTTCC AGACTACCAA TCAACCGGGC GGGAACCTGG 
AAGCGTGAAC ACAGCAAACA TTCCTCGTCC CTTCCTCGAG TGAGTACTTG GAGAAGAACT 
GGAAGCTCAT CTTCTATTCT TTCTGCTTCA TCAGAGTCCA GTGAAAAAGC AAAAAGTGAG 
GATGAAAGGC ATGTGAGCTC CATGCCAGCA CCCAGACAGA TGAAGGAAAA CCAGGTGCCC 
ACCAAAGGAA CATGGAGGAA AATCAAGGAA AGTGACATTT CTCCCACAGG CATGGCTTCT 
CAGAGCGCTT CCTCAGGTGC TGCCAGTGGT GCTGAATCCA AGCCTCTGAT CTATCAGATG 
GCACCTCCTG TCTCTAAAAC AGAGGATGTT TGGGTGAGAA TTGAGGACTG CCCCATTAAC 
AACCCTAGAT CTGGACGGTC CCCCACAGGC AACACCCCCC CAGTGATTGA CAGTGTTTCA 
GAGAAGGGAA GTTCAAGCAT TAAAGATTCA AAAGACACCC ATGGGAAACA GAGTGTGGGC 
AGTGGCAGTC CTGTGCAAAC CGTGGGTCTG GAAACCCGCC TCAACTCCTT TGTTCAGGTA 
GAGGCCCCAG AACAGAAAGG AACTGAGGCA AAACCAGGAC AGAGTAACCC AGTCTCTATA 
GCAGAGACTG CTGAGACGTG TATAGCAGAG CGTACCCCTT TCAGTTCCAG TAGCTCCAGC 
AAGCACAGCT CACCTAGCGG GACTGTTGCT GCCAGAGTGA CACCTTTTAA TTACAACCCT 
AGCCCTAGGA AGAGCAGCGC AGACAGCACT TCAGCCCGGC CGTCTCAGAT CCCTACGCCA 
GTGAGCACCA ACACGAAGAA GAGAGATTCG AAGACTGACA GCACAGAATC CAGTGGAGCC 
CAAAGTCCTA AACGCCATTC CGGGTCTTAC CTCGTGACGT CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525534.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525597.1 Mus musculus adenomatosis polyposis coli (Apc), transcript variant X6, mRNA.

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CloneID	OMu54321
Clone ID Related Accession (Same CDS sequence)	XM_006525534.4 , XM_006525534.3
Accession Version	XM_006525534.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525534.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGTCCAG CTACACGTGT GGATCACGAA 
ACAGCCAGTG TTTTGAGTTC TAGCGGCACG CACTCTGCTC CTCGAAGGTT GACAAGTCAT 
CTGGGGACAA AGGTGGAAAT GGTGTATTCC TTGTTGTCAA TGCTTGGTAC TCATGATAAG 
GACGATATGT CACGAACTTT GCTAGCTATG TCCAGCTCCC AAGACAGCTG TATATCCATG 
CGGCAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT 
GTATTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC 
CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT 
CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAC 
GAACAAGGCA TGGACCAGGA CAAAAACCCA ATGCCAGCTC CTGTTGAGCA TCAGATCTGT 
CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA TTTGATGAAG AGCATAGGCA TGCAATGAAT 
GAACTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGTCA GGGGCTTTCA 
GGGGGACTGC AGGCCATTGC AGAGTTATTG CAGGTGGACT GTGAGATGTA TGGGCTTACT 
AATGACCACT ACAGTGTTAC TTTAAGACGG TATGCTGGAA TGGCTTTGAC AAACTTGACC 
TTTGGAGATG TTGCCAACAA GGCTACGCTG TGTTCTATGA AAGGCTGCAT GAGAGCACTT 
GTGGCCCAGT TAAAATCTGA GAGTGAAGAC TTACAGCAGG TTATTGCAAG TGTTTTGAGG 
AATTTGTCTT GGCGAGCAGA TGTAAATAGC AAAAAGACGT TGAGAGAAGT TGGAAGTGTG 
AAAGCATTGA TGGAATGTGC TTTGGAAGTT AAAAAGGAAT CAACCCTCAA AAGCGTTTTG 
AGTGCCTTAT GGAACCTGTC TGCACACTGC ACTGAGAATA AGGCTGACAT CTGTGCTGTG 
GATGGAGCAC TGGCATTTCT GGTTGGCACC CTCACTTACC GGAGCCAGAC AAATACTTTA 
GCCATTATTG AAAGTGGAGG TGGGATATTA CGGAATGTGT CCAGCTTGAT AGCTACAAAC 
GAAGACCACA GGCAAATCCT AAGAGAGAAC AATTGCCTAC AAACTTTATT ACAGCACTTG 
AAATCTCACA GCTTGACAAT AGTCAGTAAT GCATGTGGAA CTTTGTGGAA TCTCTCAGCA 
AGAAATCCTA AAGACCAGGA AGCCTTGTGG GACATGGGGG CAGTGAGCAT GCTCAAGAAC 
CTCATTCATT CCAAGCACAA AATGATTGCC ATGGGAAGTG CAGCAGCTTT AAGGAATCTC 
ATGGCAAACA GACCTGCAAA GTATAAGGAT GCCAATATCA TGTCTCCCGG CTCAAGTCTG 
CCATCCCTTC ACGTTAGGAA ACAGAAAGCT CTAGAAGCTG AGCTAGATGC TCAGCATTTA 
TCAGAAACCT TCGACAACAT TGACAACCTA AGTCCCAAGG CCTCTCACCG GAGTAAGCAG 
AGACACAAGC AGAATCTTTA TGGTGACTAT GCTTTTGACG CCAATCGACA TGATGATAGT 
AGGTCAGACA ATTTCAATAC TGGAAACATG ACTGTTCTTT CACCATATTT AAATACTACG 
GTATTGCCCA GCTCTTCTTC CTCAAGGGGA AGTTTAGACA GTTCTCGTTC TGAGAAAGAC 
AGAAGTTTGG AGAGAGAGCG AGGTATTGGC CTCAGTGCTT ACCATCCAAC AACAGAAAAT 
GCAGGAACCT CATCAAAACG AGGTCTGCAG ATCACTACCA CTGCAGCCCA GATAGCCAAA 
GTTATGGAAG AAGTATCAGC CATTCATACC TCCCAGGACG ACAGAAGTTC TGCTTCTACC 
ACCGAGTTCC ATTGTGTGGC AGACGACAGG AGTGCGGCAC GAAGAAGCTC TGCCTCCCAC 
ACACACTCAA ACACATACAA CTTCACTAAG TCGGAAAATT CAAATAGGAC ATGCTCTATG 
CCTTATGCCA AAGTGGAATA TAAACGATCT TCAAATGACA GTTTAAATAG TGTCACTAGT 
AGTGATGGAT ATGGTAAAAG AGGCCAAATG AAACCCTCAG TTGAATCCTA TTCTGAAGAT 
GATGAAAGTA AATTTTGCAG TTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAGATACAC 
AGTGCAAATC ATATGGATGA TAATGATGGA GAACTGGATA CACCAATAAA TTACAGTCTT 
AAATATTCAG ATGAGCAGTT GAACTCAGGA AGGCAGAGTC CCTCACAGAA TGAAAGGTGG 
GCAAGACCAA AGCATGTGAT AGAAGATGAA ATAAAGCAAA ACGAGCAAAG ACAAGCAAGA 
AGCCAGAACA CCAGTTATCC TGTCTATTCT GAGAATACCG ATGACAAACA CCTCAAATTC 
CAACCACATT TTGGACAACA AGAATGTGTT TCCCCATATA GGTCAAGGGG AACCAGTGGT 
TCAGAAACAA ATCGAATGGG TTCTAGTCAT GCAATTAATC AAAATGTAAA CCAGTCTCTG 
TGTCAGGAAG ATGATTATGA AGATGATAAA CCTACCAACT ACAGTGAACG TTATTCTGAG 
GAAGAACAAC ATGAAGAAGA AGAAGAGAGA CCGACAAATT ATAGCATAAA ATATAATGAA 
GAGAAACATC ATGTGGATCA GCCTATTGAT TATAGTTTAA AATATGCCAC TGACATTTCT 
TCCTCACAAA AACCATCATT TTCATTCTCA AAGAATTCAT CAGCACAAAG CACTAAACCT 
GAACATCTCT CTCCAAGCAG CGAGAATACA GCTGTACCTC CATCTAATGC CAAAAGGCAG 
AATCAGCTGC GTCCAAGTTC AGCACAAAGA AATGGCCAGA CTCAAAAAGG CACTACTTGC 
AAAGTCCCCT CCATCAACCA AGAAACAATA CAGACTTACT GCGTAGAAGA CACCCCAATA 
TGTTTTTCAA GGTGCAGTTC ATTATCATCA CTGTCATCAG CTGACGATGA AATAGGATGT 
GATCAGACAA CACAGGAAGC AGATTCTGCT AATACTCTGC AGACAGCAGA AGTAAAAGAG 
AATGATGTAA CTCGGTCAGC TGAAGATCCT GCAACTGAAG TTCCAGCAGT GTCCCAGAAT 
GCTAGAGCCA AACCCAGCCG ACTCCAGGCT TCTGGCTTAT CTTCAGAATC AACCAGGCAT 
AATAAAGCTG TTGAGTTTTC TTCAGGAGCC AAGTCTCCCT CCAAAAGTGG TGCTCAGACA 
CCCAAAAGTC CCCCAGAACA CTATGTCCAG GAGACTCCGC TCGTATTCAG CAGGTGTACT 
TCTGTCAGCT CCCTTGACAG TTTTGAGAGT CGCTCCATTG CCAGCTCTGT TCAGAGTGAG 
CCATGTAGTG GAATGGTGAG TGGCATCATA AGCCCCAGTG ACCTTCCAGA TAGTCCTGGG 
CAGACCATGC CACCAAGCAG AAGCAAAACC CCTCCACCTC CTCCACAGAC AGTGCAGGCC 
AAGAGAGAGG TGCCAAAAAG TAAAGTCCCT GCTGCTGAGA AGAGAGAGAG TGGGCCTAAG 
CAGACTGCTG TAAATGCTGC CGTGCAGAGG GTGCAGGTCC TTCCAGACGT GGATACTTTG 
TTACACTTCG CCACAGAAAG TACTCCAGAC GGGTTTTCTT GTTCCTCCAG CCTAAGTGCT 
CTGAGCCTGG ATGAGCCATT TATACAGAAA GATGTAGAAT TAAGAATCAT GCCTCCAGTT 
CAGGAAAACG ACAATGGGAA TGAAACTGAA TCAGAACAGC CTGAGGAATC AAATGAAAAC 
CAGGATAAAG AGGTAGAAAA GCCTGACTCT GAAAAAGACT TATTAGATGA TTCTGATGAC 
GATGATATTG AAATATTAGA AGAATGTATT ATTTCAGCCA TGCCAACAAA GTCATCACGC 
AAAGCCAAAA AACTAGCCCA GACTGCTTCA AAATTACCTC CACCTGTGGC AAGGAAACCA 
AGTCAGCTAC CTGTGTATAA ACTTCTGCCA GCACAGAATA GGCTGCAGGC ACAAAAACAT 
GTTAGCTTTA CACCAGGGGA TGATGTGCCC CGGGTGTACT GTGTAGAAGG GACACCTATA 
AACTTTTCCA CAGCAACGTC TCTAAGTGAT CTGACAATAG AGTCCCCTCC AAATGAATTG 
GCTACTGGAG ATGGGGTCAG AGCGGGTATA CAGTCAGGTG AATTTGAAAA ACGAGATACC 
ATTCCTACAG AAGGCAGAAG TACAGATGAT GCTCAGCGAG GAAAAATCTC ATCTATAGTT 
ACACCAGACC TGGATGACAA CAAAGCAGAG GAAGGAGATA TTCTTGCAGA ATGTATCAAT 
TCTGCTATGC CCAAAGGAAA AAGCCACAAG CCTTTCCGAG TGAAAAAGAT AATGGACCAA 
GTCCAACAAG CATCCTCGAC TTCATCTGGA GCTAACAAAA ATCAAGTAGA CACTAAGAAA 
AAGAAGCCTA CTTCACCAGT AAAGCCCATG CCACAAAATA CTGAATATAG AACGCGTGTG 
AGAAAGAATA CAGACTCAAA AGTTAATGTA AATACTGAAG AAACTTTCTC AGACAACAAA 
GACTCAAAGA AACCAAGCTT ACAAACCAAT GCCAAGGCCT TCAATGAAAA GCTACCTAAC 
AATGAAGACA GAGTGCGGGG GAGCTTCGCC TTGGACTCAC CGCATCACTA CACCCCTATT 
GAGGGGACGC CGTACTGCTT TTCCCGAAAT GACTCCTTGA GTTCTCTGGA TTTTGATGAT 
GACGATGTTG ACCTTTCCAG GGAAAAGGCC GAGTTAAGAA AGGGCAAAGA AAGCAAGGAT 
TCCGAAGCCA AAGTTACCTG CCGCCCAGAA CCAAACTCAA GCCAGCAGGC AGCTAGTAAG 
TCACAAGCCA GTATAAAACA TCCAGCAAAC AGAGCACAGT CCAAACCAGT GCTGCAGAAA 
CAGCCCACTT TCCCCCAGTC CTCCAAAGAC GGACCAGATA GAGGGGCAGC AACTGACGAA 
AAACTGCAGA ATTTTGCTAT TGAAAATACT CCAGTTTGCT TTTCTCGAAA TTCCTCTCTG 
AGTTCCCTTA GTGACATTGA CCAGGAAAAC AACAATAACA AAGAAAGTGA ACCAATCAAA 
GAAGCTGAAC CTGCCAACTC ACAAGGAGAG CCCAGTAAGC CTCAGGCATC CGGGTATGCT 
CCCAAGTCCT TCCACGTCGA AGACACCCCT GTCTGTTTCT CAAGAAACAG CTCTCTCAGT 
TCTCTTAGCA TTGACTCTGA GGACGACCTG TTACAGGAGT GTATAAGTTC TGCCATGCCA 
AAAAAGAAAA GGCCTTCAAG ACTCAAGAGT GAGAGCGAAA AGCAGAGCCC TAGAAAAGTG 
GGTGGCATAT TAGCTGAAGA CCTGACGCTT GATTTGAAAG ATCTACAGAG GCCAGATTCA 
GAACACGCTT TCTCCCCCGA CTCAGAAAAT TTTGACTGGA AAGCTATTCA GGAAGGCGCA 
AACTCCATAG TAAGTAGTTT GCACCAAGCT GCTGCAGCCG CCGCGTGCTT ATCTAGACAA 
GCGTCATCCG ACTCAGATTC CATTCTGTCA CTAAAGTCCG GCATTTCTCT GGGATCGCCT 
TTTCATCTTA CACCTGATCA AGAGGAAAAG CCATTCACAA GCAATAAAGG CCCAAGAATT 
CTCAAACCTG GAGAGAAAAG CACATTAGAA GCAAAAAAAA TAGAATCTGA AAACAAAGGA 
ATCAAAGGCG GGAAAAAGGT TTATAAAAGC TTGATTACGG GAAAGATTCG CTCCAATTCA 
GAAATTTCCA GCCAAATGAA ACAACCCCTC CCGACAAACA TGCCTTCAAT CTCAAGAGGC 
AGGACGATGA TTCACATCCC AGGGCTTCGG AATAGCTCCT CTAGTACAAG CCCTGTCTCT 
AAGAAAGGCC CACCCCTCAA GACTCCAGCC TCTAAAAGCC CCAGTGAAGG GCCGGGAGCT 
ACCACTTCTC CTCGAGGAAC TAAGCCAGCA GGAAAGTCAG AGCTTAGCCC TATCACCAGG 
CAAACTTCCC AAATCAGTGG GTCAAATAAG GGGTCTTCTA GATCAGGATC TAGAGACTCC 
ACTCCCTCAA GACCTACACA GCAACCATTA AGTAGGCCAA TGCAGTCTCC AGGGCGAAAC 
TCAATTTCCC CTGGTAGAAA TGGAATAAGC CCTCCTAACA AACTGTCTCA GCTGCCCAGA 
ACATCATCTC CCAGTACTGC TTCAACTAAG TCCTCCGGTT CTGGGAAAAT GTCATATACA 
TCCCCAGGTA GACAGCTGAG CCAACAAAAT CTTACCAAAC AAGCAAGTTT ATCCAAGAAT 
GCCAGCAGTA TCCCCAGAAG TGAGTCGGCA TCTAAAGGAC TGAATCAGAT GAGTAACGGC 
AATGGGTCAA ATAAAAAGGT AGAACTTTCT AGAATGTCTT CAACTAAATC AAGTGGAAGT 
GAATCAGACA GATCAGAAAG GCCTGCATTA GTACGCCAGT CTACTTTCAT CAAAGAAGCC 
CCAAGCCCAA CCCTGAGGAG GAAACTGGAG GAATCTGCCT CATTTGAATC CCTTTCTCCA 
TCTTCTAGAC CAGATTCTCC CACCAGGTCG CAGGCACAGA CCCCAGTTTT AAGCCCTTCC 
CTTCCTGATA TGTCTCTGTC CACACATCCA TCTGTTCAGG CAGGTGGGTG GCGAAAGCTC 
CCGCCTAATC TCAGCCCCAC TATCGAGTAT AATGACGGAA GGCCCACAAA ACGGCATGAT 
ATTGCACGCT CCCATTCTGA AAGTCCTTCC AGACTACCAA TCAACCGGGC GGGAACCTGG 
AAGCGTGAAC ACAGCAAACA TTCCTCGTCC CTTCCTCGAG TGAGTACTTG GAGAAGAACT 
GGAAGCTCAT CTTCTATTCT TTCTGCTTCA TCAGAGTCCA GTGAAAAAGC AAAAAGTGAG 
GATGAAAGGC ATGTGAGCTC CATGCCAGCA CCCAGACAGA TGAAGGAAAA CCAGGTGCCC 
ACCAAAGGAA CATGGAGGAA AATCAAGGAA AGTGACATTT CTCCCACAGG CATGGCTTCT 
CAGAGCGCTT CCTCAGGTGC TGCCAGTGGT GCTGAATCCA AGCCTCTGAT CTATCAGATG 
GCACCTCCTG TCTCTAAAAC AGAGGATGTT TGGGTGAGAA TTGAGGACTG CCCCATTAAC 
AACCCTAGAT CTGGACGGTC CCCCACAGGC AACACCCCCC CAGTGATTGA CAGTGTTTCA 
GAGAAGGGAA GTTCAAGCAT TAAAGATTCA AAAGACACCC ATGGGAAACA GAGTGTGGGC 
AGTGGCAGTC CTGTGCAAAC CGTGGGTCTG GAAACCCGCC TCAACTCCTT TGTTCAGGTA 
GAGGCCCCAG AACAGAAAGG AACTGAGGCA AAACCAGGAC AGAGTAACCC AGTCTCTATA 
GCAGAGACTG CTGAGACGTG TATAGCAGAG CGTACCCCTT TCAGTTCCAG TAGCTCCAGC 
AAGCACAGCT CACCTAGCGG GACTGTTGCT GCCAGAGTGA CACCTTTTAA TTACAACCCT 
AGCCCTAGGA AGAGCAGCGC AGACAGCACT TCAGCCCGGC CGTCTCAGAT CCCTACGCCA 
GTGAGCACCA ACACGAAGAA GAGAGATTCG AAGACTGACA GCACAGAATC CAGTGGAGCC 
CAAAGTCCTA AACGCCATTC CGGGTCTTAC CTCGTGACGT CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525597.1
CDS	496..9003
Translation	MYAFLRSGPVAALPASVPPVTLGARSSGGGRGRVPGEGRRPGGARACGRGASVWQEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAESPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525534.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525534.3

ORF Insert Sequence:

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGTCCAG CTACACGTGT GGATCACGAA 
ACAGCCAGTG TTTTGAGTTC TAGCGGCACG CACTCTGCTC CTCGAAGGTT GACAAGTCAT 
CTGGGGACAA AGGTGGAAAT GGTGTATTCC TTGTTGTCAA TGCTTGGTAC TCATGATAAG 
GACGATATGT CACGAACTTT GCTAGCTATG TCCAGCTCCC AAGACAGCTG TATATCCATG 
CGGCAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT 
GTATTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC 
CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT 
CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAC 
GAACAAGGCA TGGACCAGGA CAAAAACCCA ATGCCAGCTC CTGTTGAGCA TCAGATCTGT 
CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA TTTGATGAAG AGCATAGGCA TGCAATGAAT 
GAACTTGGTA GGAAGGCTAC CCGGGGCATT TCATCCCAGG AGCTAGGTCA GGGGCTTTCA 
GGGGGACTGC AGGCCATTGC AGAGTTATTG CAGGTGGACT GTGAGATGTA TGGGCTTACT 
AATGACCACT ACAGTGTTAC TTTAAGACGG TATGCTGGAA TGGCTTTGAC AAACTTGACC 
TTTGGAGATG TTGCCAACAA GGCTACGCTG TGTTCTATGA AAGGCTGCAT GAGAGCACTT 
GTGGCCCAGT TAAAATCTGA GAGTGAAGAC TTACAGCAGG TTATTGCAAG TGTTTTGAGG 
AATTTGTCTT GGCGAGCAGA TGTAAATAGC AAAAAGACGT TGAGAGAAGT TGGAAGTGTG 
AAAGCATTGA TGGAATGTGC TTTGGAAGTT AAAAAGGAAT CAACCCTCAA AAGCGTTTTG 
AGTGCCTTAT GGAACCTGTC TGCACACTGC ACTGAGAATA AGGCTGACAT CTGTGCTGTG 
GATGGAGCAC TGGCATTTCT GGTTGGCACC CTCACTTACC GGAGCCAGAC AAATACTTTA 
GCCATTATTG AAAGTGGAGG TGGGATATTA CGGAATGTGT CCAGCTTGAT AGCTACAAAC 
GAAGACCACA GGCAAATCCT AAGAGAGAAC AATTGCCTAC AAACTTTATT ACAGCACTTG 
AAATCTCACA GCTTGACAAT AGTCAGTAAT GCATGTGGAA CTTTGTGGAA TCTCTCAGCA 
AGAAATCCTA AAGACCAGGA AGCCTTGTGG GACATGGGGG CAGTGAGCAT GCTCAAGAAC 
CTCATTCATT CCAAGCACAA AATGATTGCC ATGGGAAGTG CAGCAGCTTT AAGGAATCTC 
ATGGCAAACA GACCTGCAAA GTATAAGGAT GCCAATATCA TGTCTCCCGG CTCAAGTCTG 
CCATCCCTTC ACGTTAGGAA ACAGAAAGCT CTAGAAGCTG AGCTAGATGC TCAGCATTTA 
TCAGAAACCT TCGACAACAT TGACAACCTA AGTCCCAAGG CCTCTCACCG GAGTAAGCAG 
AGACACAAGC AGAATCTTTA TGGTGACTAT GCTTTTGACG CCAATCGACA TGATGATAGT 
AGGTCAGACA ATTTCAATAC TGGAAACATG ACTGTTCTTT CACCATATTT AAATACTACG 
GTATTGCCCA GCTCTTCTTC CTCAAGGGGA AGTTTAGACA GTTCTCGTTC TGAGAAAGAC 
AGAAGTTTGG AGAGAGAGCG AGGTATTGGC CTCAGTGCTT ACCATCCAAC AACAGAAAAT 
GCAGGAACCT CATCAAAACG AGGTCTGCAG ATCACTACCA CTGCAGCCCA GATAGCCAAA 
GTTATGGAAG AAGTATCAGC CATTCATACC TCCCAGGACG ACAGAAGTTC TGCTTCTACC 
ACCGAGTTCC ATTGTGTGGC AGACGACAGG AGTGCGGCAC GAAGAAGCTC TGCCTCCCAC 
ACACACTCAA ACACATACAA CTTCACTAAG TCGGAAAATT CAAATAGGAC ATGCTCTATG 
CCTTATGCCA AAGTGGAATA TAAACGATCT TCAAATGACA GTTTAAATAG TGTCACTAGT 
AGTGATGGAT ATGGTAAAAG AGGCCAAATG AAACCCTCAG TTGAATCCTA TTCTGAAGAT 
GATGAAAGTA AATTTTGCAG TTATGGTCAG TATCCAGCTG ACCTAGCCCA TAAGATACAC 
AGTGCAAATC ATATGGATGA TAATGATGGA GAACTGGATA CACCAATAAA TTACAGTCTT 
AAATATTCAG ATGAGCAGTT GAACTCAGGA AGGCAGAGTC CCTCACAGAA TGAAAGGTGG 
GCAAGACCAA AGCATGTGAT AGAAGATGAA ATAAAGCAAA ACGAGCAAAG ACAAGCAAGA 
AGCCAGAACA CCAGTTATCC TGTCTATTCT GAGAATACCG ATGACAAACA CCTCAAATTC 
CAACCACATT TTGGACAACA AGAATGTGTT TCCCCATATA GGTCAAGGGG AACCAGTGGT 
TCAGAAACAA ATCGAATGGG TTCTAGTCAT GCAATTAATC AAAATGTAAA CCAGTCTCTG 
TGTCAGGAAG ATGATTATGA AGATGATAAA CCTACCAACT ACAGTGAACG TTATTCTGAG 
GAAGAACAAC ATGAAGAAGA AGAAGAGAGA CCGACAAATT ATAGCATAAA ATATAATGAA 
GAGAAACATC ATGTGGATCA GCCTATTGAT TATAGTTTAA AATATGCCAC TGACATTTCT 
TCCTCACAAA AACCATCATT TTCATTCTCA AAGAATTCAT CAGCACAAAG CACTAAACCT 
GAACATCTCT CTCCAAGCAG CGAGAATACA GCTGTACCTC CATCTAATGC CAAAAGGCAG 
AATCAGCTGC GTCCAAGTTC AGCACAAAGA AATGGCCAGA CTCAAAAAGG CACTACTTGC 
AAAGTCCCCT CCATCAACCA AGAAACAATA CAGACTTACT GCGTAGAAGA CACCCCAATA 
TGTTTTTCAA GGTGCAGTTC ATTATCATCA CTGTCATCAG CTGACGATGA AATAGGATGT 
GATCAGACAA CACAGGAAGC AGATTCTGCT AATACTCTGC AGACAGCAGA AGTAAAAGAG 
AATGATGTAA CTCGGTCAGC TGAAGATCCT GCAACTGAAG TTCCAGCAGT GTCCCAGAAT 
GCTAGAGCCA AACCCAGCCG ACTCCAGGCT TCTGGCTTAT CTTCAGAATC AACCAGGCAT 
AATAAAGCTG TTGAGTTTTC TTCAGGAGCC AAGTCTCCCT CCAAAAGTGG TGCTCAGACA 
CCCAAAAGTC CCCCAGAACA CTATGTCCAG GAGACTCCGC TCGTATTCAG CAGGTGTACT 
TCTGTCAGCT CCCTTGACAG TTTTGAGAGT CGCTCCATTG CCAGCTCTGT TCAGAGTGAG 
CCATGTAGTG GAATGGTGAG TGGCATCATA AGCCCCAGTG ACCTTCCAGA TAGTCCTGGG 
CAGACCATGC CACCAAGCAG AAGCAAAACC CCTCCACCTC CTCCACAGAC AGTGCAGGCC 
AAGAGAGAGG TGCCAAAAAG TAAAGTCCCT GCTGCTGAGA AGAGAGAGAG TGGGCCTAAG 
CAGACTGCTG TAAATGCTGC CGTGCAGAGG GTGCAGGTCC TTCCAGACGT GGATACTTTG 
TTACACTTCG CCACAGAAAG TACTCCAGAC GGGTTTTCTT GTTCCTCCAG CCTAAGTGCT 
CTGAGCCTGG ATGAGCCATT TATACAGAAA GATGTAGAAT TAAGAATCAT GCCTCCAGTT 
CAGGAAAACG ACAATGGGAA TGAAACTGAA TCAGAACAGC CTGAGGAATC AAATGAAAAC 
CAGGATAAAG AGGTAGAAAA GCCTGACTCT GAAAAAGACT TATTAGATGA TTCTGATGAC 
GATGATATTG AAATATTAGA AGAATGTATT ATTTCAGCCA TGCCAACAAA GTCATCACGC 
AAAGCCAAAA AACTAGCCCA GACTGCTTCA AAATTACCTC CACCTGTGGC AAGGAAACCA 
AGTCAGCTAC CTGTGTATAA ACTTCTGCCA GCACAGAATA GGCTGCAGGC ACAAAAACAT 
GTTAGCTTTA CACCAGGGGA TGATGTGCCC CGGGTGTACT GTGTAGAAGG GACACCTATA 
AACTTTTCCA CAGCAACGTC TCTAAGTGAT CTGACAATAG AGTCCCCTCC AAATGAATTG 
GCTACTGGAG ATGGGGTCAG AGCGGGTATA CAGTCAGGTG AATTTGAAAA ACGAGATACC 
ATTCCTACAG AAGGCAGAAG TACAGATGAT GCTCAGCGAG GAAAAATCTC ATCTATAGTT 
ACACCAGACC TGGATGACAA CAAAGCAGAG GAAGGAGATA TTCTTGCAGA ATGTATCAAT 
TCTGCTATGC CCAAAGGAAA AAGCCACAAG CCTTTCCGAG TGAAAAAGAT AATGGACCAA 
GTCCAACAAG CATCCTCGAC TTCATCTGGA GCTAACAAAA ATCAAGTAGA CACTAAGAAA 
AAGAAGCCTA CTTCACCAGT AAAGCCCATG CCACAAAATA CTGAATATAG AACGCGTGTG 
AGAAAGAATA CAGACTCAAA AGTTAATGTA AATACTGAAG AAACTTTCTC AGACAACAAA 
GACTCAAAGA AACCAAGCTT ACAAACCAAT GCCAAGGCCT TCAATGAAAA GCTACCTAAC 
AATGAAGACA GAGTGCGGGG GAGCTTCGCC TTGGACTCAC CGCATCACTA CACCCCTATT 
GAGGGGACGC CGTACTGCTT TTCCCGAAAT GACTCCTTGA GTTCTCTGGA TTTTGATGAT 
GACGATGTTG ACCTTTCCAG GGAAAAGGCC GAGTTAAGAA AGGGCAAAGA AAGCAAGGAT 
TCCGAAGCCA AAGTTACCTG CCGCCCAGAA CCAAACTCAA GCCAGCAGGC AGCTAGTAAG 
TCACAAGCCA GTATAAAACA TCCAGCAAAC AGAGCACAGT CCAAACCAGT GCTGCAGAAA 
CAGCCCACTT TCCCCCAGTC CTCCAAAGAC GGACCAGATA GAGGGGCAGC AACTGACGAA 
AAACTGCAGA ATTTTGCTAT TGAAAATACT CCAGTTTGCT TTTCTCGAAA TTCCTCTCTG 
AGTTCCCTTA GTGACATTGA CCAGGAAAAC AACAATAACA AAGAAAGTGA ACCAATCAAA 
GAAGCTGAAC CTGCCAACTC ACAAGGAGAG CCCAGTAAGC CTCAGGCATC CGGGTATGCT 
CCCAAGTCCT TCCACGTCGA AGACACCCCT GTCTGTTTCT CAAGAAACAG CTCTCTCAGT 
TCTCTTAGCA TTGACTCTGA GGACGACCTG TTACAGGAGT GTATAAGTTC TGCCATGCCA 
AAAAAGAAAA GGCCTTCAAG ACTCAAGAGT GAGAGCGAAA AGCAGAGCCC TAGAAAAGTG 
GGTGGCATAT TAGCTGAAGA CCTGACGCTT GATTTGAAAG ATCTACAGAG GCCAGATTCA 
GAACACGCTT TCTCCCCCGA CTCAGAAAAT TTTGACTGGA AAGCTATTCA GGAAGGCGCA 
AACTCCATAG TAAGTAGTTT GCACCAAGCT GCTGCAGCCG CCGCGTGCTT ATCTAGACAA 
GCGTCATCCG ACTCAGATTC CATTCTGTCA CTAAAGTCCG GCATTTCTCT GGGATCGCCT 
TTTCATCTTA CACCTGATCA AGAGGAAAAG CCATTCACAA GCAATAAAGG CCCAAGAATT 
CTCAAACCTG GAGAGAAAAG CACATTAGAA GCAAAAAAAA TAGAATCTGA AAACAAAGGA 
ATCAAAGGCG GGAAAAAGGT TTATAAAAGC TTGATTACGG GAAAGATTCG CTCCAATTCA 
GAAATTTCCA GCCAAATGAA ACAACCCCTC CCGACAAACA TGCCTTCAAT CTCAAGAGGC 
AGGACGATGA TTCACATCCC AGGGCTTCGG AATAGCTCCT CTAGTACAAG CCCTGTCTCT 
AAGAAAGGCC CACCCCTCAA GACTCCAGCC TCTAAAAGCC CCAGTGAAGG GCCGGGAGCT 
ACCACTTCTC CTCGAGGAAC TAAGCCAGCA GGAAAGTCAG AGCTTAGCCC TATCACCAGG 
CAAACTTCCC AAATCAGTGG GTCAAATAAG GGGTCTTCTA GATCAGGATC TAGAGACTCC 
ACTCCCTCAA GACCTACACA GCAACCATTA AGTAGGCCAA TGCAGTCTCC AGGGCGAAAC 
TCAATTTCCC CTGGTAGAAA TGGAATAAGC CCTCCTAACA AACTGTCTCA GCTGCCCAGA 
ACATCATCTC CCAGTACTGC TTCAACTAAG TCCTCCGGTT CTGGGAAAAT GTCATATACA 
TCCCCAGGTA GACAGCTGAG CCAACAAAAT CTTACCAAAC AAGCAAGTTT ATCCAAGAAT 
GCCAGCAGTA TCCCCAGAAG TGAGTCGGCA TCTAAAGGAC TGAATCAGAT GAGTAACGGC 
AATGGGTCAA ATAAAAAGGT AGAACTTTCT AGAATGTCTT CAACTAAATC AAGTGGAAGT 
GAATCAGACA GATCAGAAAG GCCTGCATTA GTACGCCAGT CTACTTTCAT CAAAGAAGCC 
CCAAGCCCAA CCCTGAGGAG GAAACTGGAG GAATCTGCCT CATTTGAATC CCTTTCTCCA 
TCTTCTAGAC CAGATTCTCC CACCAGGTCG CAGGCACAGA CCCCAGTTTT AAGCCCTTCC 
CTTCCTGATA TGTCTCTGTC CACACATCCA TCTGTTCAGG CAGGTGGGTG GCGAAAGCTC 
CCGCCTAATC TCAGCCCCAC TATCGAGTAT AATGACGGAA GGCCCACAAA ACGGCATGAT 
ATTGCACGCT CCCATTCTGA AAGTCCTTCC AGACTACCAA TCAACCGGGC GGGAACCTGG 
AAGCGTGAAC ACAGCAAACA TTCCTCGTCC CTTCCTCGAG TGAGTACTTG GAGAAGAACT 
GGAAGCTCAT CTTCTATTCT TTCTGCTTCA TCAGAGTCCA GTGAAAAAGC AAAAAGTGAG 
GATGAAAGGC ATGTGAGCTC CATGCCAGCA CCCAGACAGA TGAAGGAAAA CCAGGTGCCC 
ACCAAAGGAA CATGGAGGAA AATCAAGGAA AGTGACATTT CTCCCACAGG CATGGCTTCT 
CAGAGCGCTT CCTCAGGTGC TGCCAGTGGT GCTGAATCCA AGCCTCTGAT CTATCAGATG 
GCACCTCCTG TCTCTAAAAC AGAGGATGTT TGGGTGAGAA TTGAGGACTG CCCCATTAAC 
AACCCTAGAT CTGGACGGTC CCCCACAGGC AACACCCCCC CAGTGATTGA CAGTGTTTCA 
GAGAAGGGAA GTTCAAGCAT TAAAGATTCA AAAGACACCC ATGGGAAACA GAGTGTGGGC 
AGTGGCAGTC CTGTGCAAAC CGTGGGTCTG GAAACCCGCC TCAACTCCTT TGTTCAGGTA 
GAGGCCCCAG AACAGAAAGG AACTGAGGCA AAACCAGGAC AGAGTAACCC AGTCTCTATA 
GCAGAGACTG CTGAGACGTG TATAGCAGAG CGTACCCCTT TCAGTTCCAG TAGCTCCAGC 
AAGCACAGCT CACCTAGCGG GACTGTTGCT GCCAGAGTGA CACCTTTTAA TTACAACCCT 
AGCCCTAGGA AGAGCAGCGC AGACAGCACT TCAGCCCGGC CGTCTCAGAT CCCTACGCCA 
GTGAGCACCA ACACGAAGAA GAGAGATTCG AAGACTGACA GCACAGAATC CAGTGGAGCC 
CAAAGTCCTA AACGCCATTC CGGGTCTTAC CTCGTGACGT CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250284.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106144.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X13, mRNA.

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CloneID	OMu54322
Clone ID Related Accession (Same CDS sequence)	XM_030250284.1 , XM_006525535.1 , XM_006525536.3 , XM_006525536.4 , XM_030250283.1
Accession Version	XM_030250284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGTAGG AAGGCTACCC GGGGCATTTC ATCCCAGGAG 
CTAGGTCAGG GGCTTTCAGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106144.1
CDS	420..8825
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250284.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250284.1

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGTAGG AAGGCTACCC GGGGCATTTC ATCCCAGGAG 
CTAGGTCAGG GGCTTTCAGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525535.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525598.1 Mus musculus adenomatosis polyposis coli (Apc), transcript variant X7, mRNA.

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CloneID	OMu54322
Clone ID Related Accession (Same CDS sequence)	XM_030250284.1 , XM_006525535.1 , XM_006525536.3 , XM_006525536.4 , XM_030250283.1
Accession Version	XM_006525535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGTAGG AAGGCTACCC GGGGCATTTC ATCCCAGGAG 
CTAGGTCAGG GGCTTTCAGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525598.1
CDS	298..8703
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525535.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525535.1

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGTAGG AAGGCTACCC GGGGCATTTC ATCCCAGGAG 
CTAGGTCAGG GGCTTTCAGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525536.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525599.1 Mus musculus adenomatosis polyposis coli (Apc), transcript variant X8, mRNA.

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CloneID	OMu54322
Clone ID Related Accession (Same CDS sequence)	XM_030250284.1 , XM_006525535.1 , XM_006525536.3 , XM_006525536.4 , XM_030250283.1
Accession Version	XM_006525536.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525536.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGTAGG AAGGCTACCC GGGGCATTTC ATCCCAGGAG 
CTAGGTCAGG GGCTTTCAGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525599.1
CDS	294..8699
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525536.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525536.3

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGTAGG AAGGCTACCC GGGGCATTTC ATCCCAGGAG 
CTAGGTCAGG GGCTTTCAGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525536.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525599.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X14, mRNA.

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CloneID	OMu54322
Clone ID Related Accession (Same CDS sequence)	XM_030250284.1 , XM_006525535.1 , XM_006525536.3 , XM_006525536.4 , XM_030250283.1
Accession Version	XM_006525536.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525536.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGTAGG AAGGCTACCC GGGGCATTTC ATCCCAGGAG 
CTAGGTCAGG GGCTTTCAGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525599.1
CDS	294..8699
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525536.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525536.4

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGTAGG AAGGCTACCC GGGGCATTTC ATCCCAGGAG 
CTAGGTCAGG GGCTTTCAGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250283.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106143.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X12, mRNA.

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CloneID	OMu54322
Clone ID Related Accession (Same CDS sequence)	XM_030250284.1 , XM_006525535.1 , XM_006525536.3 , XM_006525536.4 , XM_030250283.1
Accession Version	XM_030250283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGTAGG AAGGCTACCC GGGGCATTTC ATCCCAGGAG 
CTAGGTCAGG GGCTTTCAGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106143.1
CDS	725..9130
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250283.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250283.1

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGTAGG AAGGCTACCC GGGGCATTTC ATCCCAGGAG 
CTAGGTCAGG GGCTTTCAGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525538.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525601.1 Mus musculus adenomatosis polyposis coli (Apc), transcript variant X10, mRNA.

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CloneID	OMu54323
Clone ID Related Accession (Same CDS sequence)	XM_006525538.3 , XM_006525538.4
Accession Version	XM_006525538.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525538.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGATA 
CGAGCTTACT GTGAAACCTG TTGGGAGTGG CAGGAAGCCC ACGAACAAGG CATGGACCAG 
GACAAAAACC CAATGCCAGC TCCTGTTGAG CATCAGATCT GTCCTGCTGT GTGTGTTCTA 
ATGAAGCTTT CATTTGATGA AGAGCATAGG CATGCAATGA ATGAACTTGG TAGGAAGGCT 
ACCCGGGGCA TTTCATCCCA GGAGCTAGGT CAGGGGCTTT CAGGGGGACT GCAGGCCATT 
GCAGAGTTAT TGCAGGTGGA CTGTGAGATG TATGGGCTTA CTAATGACCA CTACAGTGTT 
ACTTTAAGAC GGTATGCTGG AATGGCTTTG ACAAACTTGA CCTTTGGAGA TGTTGCCAAC 
AAGGCTACGC TGTGTTCTAT GAAAGGCTGC ATGAGAGCAC TTGTGGCCCA GTTAAAATCT 
GAGAGTGAAG ACTTACAGCA GGTTATTGCA AGTGTTTTGA GGAATTTGTC TTGGCGAGCA 
GATGTAAATA GCAAAAAGAC GTTGAGAGAA GTTGGAAGTG TGAAAGCATT GATGGAATGT 
GCTTTGGAAG TTAAAAAGGA ATCAACCCTC AAAAGCGTTT TGAGTGCCTT ATGGAACCTG 
TCTGCACACT GCACTGAGAA TAAGGCTGAC ATCTGTGCTG TGGATGGAGC ACTGGCATTT 
CTGGTTGGCA CCCTCACTTA CCGGAGCCAG ACAAATACTT TAGCCATTAT TGAAAGTGGA 
GGTGGGATAT TACGGAATGT GTCCAGCTTG ATAGCTACAA ACGAAGACCA CAGGCAAATC 
CTAAGAGAGA ACAATTGCCT ACAAACTTTA TTACAGCACT TGAAATCTCA CAGCTTGACA 
ATAGTCAGTA ATGCATGTGG AACTTTGTGG AATCTCTCAG CAAGAAATCC TAAAGACCAG 
GAAGCCTTGT GGGACATGGG GGCAGTGAGC ATGCTCAAGA ACCTCATTCA TTCCAAGCAC 
AAAATGATTG CCATGGGAAG TGCAGCAGCT TTAAGGAATC TCATGGCAAA CAGACCTGCA 
AAGTATAAGG ATGCCAATAT CATGTCTCCC GGCTCAAGTC TGCCATCCCT TCACGTTAGG 
AAACAGAAAG CTCTAGAAGC TGAGCTAGAT GCTCAGCATT TATCAGAAAC CTTCGACAAC 
ATTGACAACC TAAGTCCCAA GGCCTCTCAC CGGAGTAAGC AGAGACACAA GCAGAATCTT 
TATGGTGACT ATGCTTTTGA CGCCAATCGA CATGATGATA GTAGGTCAGA CAATTTCAAT 
ACTGGAAACA TGACTGTTCT TTCACCATAT TTAAATACTA CGGTATTGCC CAGCTCTTCT 
TCCTCAAGGG GAAGTTTAGA CAGTTCTCGT TCTGAGAAAG ACAGAAGTTT GGAGAGAGAG 
CGAGGTATTG GCCTCAGTGC TTACCATCCA ACAACAGAAA ATGCAGGAAC CTCATCAAAA 
CGAGGTCTGC AGATCACTAC CACTGCAGCC CAGATAGCCA AAGTTATGGA AGAAGTATCA 
GCCATTCATA CCTCCCAGGA CGACAGAAGT TCTGCTTCTA CCACCGAGTT CCATTGTGTG 
GCAGACGACA GGAGTGCGGC ACGAAGAAGC TCTGCCTCCC ACACACACTC AAACACATAC 
AACTTCACTA AGTCGGAAAA TTCAAATAGG ACATGCTCTA TGCCTTATGC CAAAGTGGAA 
TATAAACGAT CTTCAAATGA CAGTTTAAAT AGTGTCACTA GTAGTGATGG ATATGGTAAA 
AGAGGCCAAA TGAAACCCTC AGTTGAATCC TATTCTGAAG ATGATGAAAG TAAATTTTGC 
AGTTATGGTC AGTATCCAGC TGACCTAGCC CATAAGATAC ACAGTGCAAA TCATATGGAT 
GATAATGATG GAGAACTGGA TACACCAATA AATTACAGTC TTAAATATTC AGATGAGCAG 
TTGAACTCAG GAAGGCAGAG TCCCTCACAG AATGAAAGGT GGGCAAGACC AAAGCATGTG 
ATAGAAGATG AAATAAAGCA AAACGAGCAA AGACAAGCAA GAAGCCAGAA CACCAGTTAT 
CCTGTCTATT CTGAGAATAC CGATGACAAA CACCTCAAAT TCCAACCACA TTTTGGACAA 
CAAGAATGTG TTTCCCCATA TAGGTCAAGG GGAACCAGTG GTTCAGAAAC AAATCGAATG 
GGTTCTAGTC ATGCAATTAA TCAAAATGTA AACCAGTCTC TGTGTCAGGA AGATGATTAT 
GAAGATGATA AACCTACCAA CTACAGTGAA CGTTATTCTG AGGAAGAACA ACATGAAGAA 
GAAGAAGAGA GACCGACAAA TTATAGCATA AAATATAATG AAGAGAAACA TCATGTGGAT 
CAGCCTATTG ATTATAGTTT AAAATATGCC ACTGACATTT CTTCCTCACA AAAACCATCA 
TTTTCATTCT CAAAGAATTC ATCAGCACAA AGCACTAAAC CTGAACATCT CTCTCCAAGC 
AGCGAGAATA CAGCTGTACC TCCATCTAAT GCCAAAAGGC AGAATCAGCT GCGTCCAAGT 
TCAGCACAAA GAAATGGCCA GACTCAAAAA GGCACTACTT GCAAAGTCCC CTCCATCAAC 
CAAGAAACAA TACAGACTTA CTGCGTAGAA GACACCCCAA TATGTTTTTC AAGGTGCAGT 
TCATTATCAT CACTGTCATC AGCTGACGAT GAAATAGGAT GTGATCAGAC AACACAGGAA 
GCAGATTCTG CTAATACTCT GCAGACAGCA GAAGTAAAAG AGAATGATGT AACTCGGTCA 
GCTGAAGATC CTGCAACTGA AGTTCCAGCA GTGTCCCAGA ATGCTAGAGC CAAACCCAGC 
CGACTCCAGG CTTCTGGCTT ATCTTCAGAA TCAACCAGGC ATAATAAAGC TGTTGAGTTT 
TCTTCAGGAG CCAAGTCTCC CTCCAAAAGT GGTGCTCAGA CACCCAAAAG TCCCCCAGAA 
CACTATGTCC AGGAGACTCC GCTCGTATTC AGCAGGTGTA CTTCTGTCAG CTCCCTTGAC 
AGTTTTGAGA GTCGCTCCAT TGCCAGCTCT GTTCAGAGTG AGCCATGTAG TGGAATGGTG 
AGTGGCATCA TAAGCCCCAG TGACCTTCCA GATAGTCCTG GGCAGACCAT GCCACCAAGC 
AGAAGCAAAA CCCCTCCACC TCCTCCACAG ACAGTGCAGG CCAAGAGAGA GGTGCCAAAA 
AGTAAAGTCC CTGCTGCTGA GAAGAGAGAG AGTGGGCCTA AGCAGACTGC TGTAAATGCT 
GCCGTGCAGA GGGTGCAGGT CCTTCCAGAC GTGGATACTT TGTTACACTT CGCCACAGAA 
AGTACTCCAG ACGGGTTTTC TTGTTCCTCC AGCCTAAGTG CTCTGAGCCT GGATGAGCCA 
TTTATACAGA AAGATGTAGA ATTAAGAATC ATGCCTCCAG TTCAGGAAAA CGACAATGGG 
AATGAAACTG AATCAGAACA GCCTGAGGAA TCAAATGAAA ACCAGGATAA AGAGGTAGAA 
AAGCCTGACT CTGAAAAAGA CTTATTAGAT GATTCTGATG ACGATGATAT TGAAATATTA 
GAAGAATGTA TTATTTCAGC CATGCCAACA AAGTCATCAC GCAAAGCCAA AAAACTAGCC 
CAGACTGCTT CAAAATTACC TCCACCTGTG GCAAGGAAAC CAAGTCAGCT ACCTGTGTAT 
AAACTTCTGC CAGCACAGAA TAGGCTGCAG GCACAAAAAC ATGTTAGCTT TACACCAGGG 
GATGATGTGC CCCGGGTGTA CTGTGTAGAA GGGACACCTA TAAACTTTTC CACAGCAACG 
TCTCTAAGTG ATCTGACAAT AGAGTCCCCT CCAAATGAAT TGGCTACTGG AGATGGGGTC 
AGAGCGGGTA TACAGTCAGG TGAATTTGAA AAACGAGATA CCATTCCTAC AGAAGGCAGA 
AGTACAGATG ATGCTCAGCG AGGAAAAATC TCATCTATAG TTACACCAGA CCTGGATGAC 
AACAAAGCAG AGGAAGGAGA TATTCTTGCA GAATGTATCA ATTCTGCTAT GCCCAAAGGA 
AAAAGCCACA AGCCTTTCCG AGTGAAAAAG ATAATGGACC AAGTCCAACA AGCATCCTCG 
ACTTCATCTG GAGCTAACAA AAATCAAGTA GACACTAAGA AAAAGAAGCC TACTTCACCA 
GTAAAGCCCA TGCCACAAAA TACTGAATAT AGAACGCGTG TGAGAAAGAA TACAGACTCA 
AAAGTTAATG TAAATACTGA AGAAACTTTC TCAGACAACA AAGACTCAAA GAAACCAAGC 
TTACAAACCA ATGCCAAGGC CTTCAATGAA AAGCTACCTA ACAATGAAGA CAGAGTGCGG 
GGGAGCTTCG CCTTGGACTC ACCGCATCAC TACACCCCTA TTGAGGGGAC GCCGTACTGC 
TTTTCCCGAA ATGACTCCTT GAGTTCTCTG GATTTTGATG ATGACGATGT TGACCTTTCC 
AGGGAAAAGG CCGAGTTAAG AAAGGGCAAA GAAAGCAAGG ATTCCGAAGC CAAAGTTACC 
TGCCGCCCAG AACCAAACTC AAGCCAGCAG GCAGCTAGTA AGTCACAAGC CAGTATAAAA 
CATCCAGCAA ACAGAGCACA GTCCAAACCA GTGCTGCAGA AACAGCCCAC TTTCCCCCAG 
TCCTCCAAAG ACGGACCAGA TAGAGGGGCA GCAACTGACG AAAAACTGCA GAATTTTGCT 
ATTGAAAATA CTCCAGTTTG CTTTTCTCGA AATTCCTCTC TGAGTTCCCT TAGTGACATT 
GACCAGGAAA ACAACAATAA CAAAGAAAGT GAACCAATCA AAGAAGCTGA ACCTGCCAAC 
TCACAAGGAG AGCCCAGTAA GCCTCAGGCA TCCGGGTATG CTCCCAAGTC CTTCCACGTC 
GAAGACACCC CTGTCTGTTT CTCAAGAAAC AGCTCTCTCA GTTCTCTTAG CATTGACTCT 
GAGGACGACC TGTTACAGGA GTGTATAAGT TCTGCCATGC CAAAAAAGAA AAGGCCTTCA 
AGACTCAAGA GTGAGAGCGA AAAGCAGAGC CCTAGAAAAG TGGGTGGCAT ATTAGCTGAA 
GACCTGACGC TTGATTTGAA AGATCTACAG AGGCCAGATT CAGAACACGC TTTCTCCCCC 
GACTCAGAAA ATTTTGACTG GAAAGCTATT CAGGAAGGCG CAAACTCCAT AGTAAGTAGT 
TTGCACCAAG CTGCTGCAGC CGCCGCGTGC TTATCTAGAC AAGCGTCATC CGACTCAGAT 
TCCATTCTGT CACTAAAGTC CGGCATTTCT CTGGGATCGC CTTTTCATCT TACACCTGAT 
CAAGAGGAAA AGCCATTCAC AAGCAATAAA GGCCCAAGAA TTCTCAAACC TGGAGAGAAA 
AGCACATTAG AAGCAAAAAA AATAGAATCT GAAAACAAAG GAATCAAAGG CGGGAAAAAG 
GTTTATAAAA GCTTGATTAC GGGAAAGATT CGCTCCAATT CAGAAATTTC CAGCCAAATG 
AAACAACCCC TCCCGACAAA CATGCCTTCA ATCTCAAGAG GCAGGACGAT GATTCACATC 
CCAGGGCTTC GGAATAGCTC CTCTAGTACA AGCCCTGTCT CTAAGAAAGG CCCACCCCTC 
AAGACTCCAG CCTCTAAAAG CCCCAGTGAA GGGCCGGGAG CTACCACTTC TCCTCGAGGA 
ACTAAGCCAG CAGGAAAGTC AGAGCTTAGC CCTATCACCA GGCAAACTTC CCAAATCAGT 
GGGTCAAATA AGGGGTCTTC TAGATCAGGA TCTAGAGACT CCACTCCCTC AAGACCTACA 
CAGCAACCAT TAAGTAGGCC AATGCAGTCT CCAGGGCGAA ACTCAATTTC CCCTGGTAGA 
AATGGAATAA GCCCTCCTAA CAAACTGTCT CAGCTGCCCA GAACATCATC TCCCAGTACT 
GCTTCAACTA AGTCCTCCGG TTCTGGGAAA ATGTCATATA CATCCCCAGG TAGACAGCTG 
AGCCAACAAA ATCTTACCAA ACAAGCAAGT TTATCCAAGA ATGCCAGCAG TATCCCCAGA 
AGTGAGTCGG CATCTAAAGG ACTGAATCAG ATGAGTAACG GCAATGGGTC AAATAAAAAG 
GTAGAACTTT CTAGAATGTC TTCAACTAAA TCAAGTGGAA GTGAATCAGA CAGATCAGAA 
AGGCCTGCAT TAGTACGCCA GTCTACTTTC ATCAAAGAAG CCCCAAGCCC AACCCTGAGG 
AGGAAACTGG AGGAATCTGC CTCATTTGAA TCCCTTTCTC CATCTTCTAG ACCAGATTCT 
CCCACCAGGT CGCAGGCACA GACCCCAGTT TTAAGCCCTT CCCTTCCTGA TATGTCTCTG 
TCCACACATC CATCTGTTCA GGCAGGTGGG TGGCGAAAGC TCCCGCCTAA TCTCAGCCCC 
ACTATCGAGT ATAATGACGG AAGGCCCACA AAACGGCATG ATATTGCACG CTCCCATTCT 
GAAAGTCCTT CCAGACTACC AATCAACCGG GCGGGAACCT GGAAGCGTGA ACACAGCAAA 
CATTCCTCGT CCCTTCCTCG AGTGAGTACT TGGAGAAGAA CTGGAAGCTC ATCTTCTATT 
CTTTCTGCTT CATCAGAGTC CAGTGAAAAA GCAAAAAGTG AGGATGAAAG GCATGTGAGC 
TCCATGCCAG CACCCAGACA GATGAAGGAA AACCAGGTGC CCACCAAAGG AACATGGAGG 
AAAATCAAGG AAAGTGACAT TTCTCCCACA GGCATGGCTT CTCAGAGCGC TTCCTCAGGT 
GCTGCCAGTG GTGCTGAATC CAAGCCTCTG ATCTATCAGA TGGCACCTCC TGTCTCTAAA 
ACAGAGGATG TTTGGGTGAG AATTGAGGAC TGCCCCATTA ACAACCCTAG ATCTGGACGG 
TCCCCCACAG GCAACACCCC CCCAGTGATT GACAGTGTTT CAGAGAAGGG AAGTTCAAGC 
ATTAAAGATT CAAAAGACAC CCATGGGAAA CAGAGTGTGG GCAGTGGCAG TCCTGTGCAA 
ACCGTGGGTC TGGAAACCCG CCTCAACTCC TTTGTTCAGG TAGAGGCCCC AGAACAGAAA 
GGAACTGAGG CAAAACCAGG ACAGAGTAAC CCAGTCTCTA TAGCAGAGAC TGCTGAGACG 
TGTATAGCAG AGCGTACCCC TTTCAGTTCC AGTAGCTCCA GCAAGCACAG CTCACCTAGC 
GGGACTGTTG CTGCCAGAGT GACACCTTTT AATTACAACC CTAGCCCTAG GAAGAGCAGC 
GCAGACAGCA CTTCAGCCCG GCCGTCTCAG ATCCCTACGC CAGTGAGCAC CAACACGAAG 
AAGAGAGATT CGAAGACTGA CAGCACAGAA TCCAGTGGAG CCCAAAGTCC TAAACGCCAT 
TCCGGGTCTT ACCTCGTGAC GTCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525601.1
CDS	495..8804
Translation	MYAFLRSGPVAALPASVPPVTLGARSSGGGRGRVPGEGRRPGGARACGRGASVWQEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525538.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525538.3

ORF Insert Sequence:

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGATA 
CGAGCTTACT GTGAAACCTG TTGGGAGTGG CAGGAAGCCC ACGAACAAGG CATGGACCAG 
GACAAAAACC CAATGCCAGC TCCTGTTGAG CATCAGATCT GTCCTGCTGT GTGTGTTCTA 
ATGAAGCTTT CATTTGATGA AGAGCATAGG CATGCAATGA ATGAACTTGG TAGGAAGGCT 
ACCCGGGGCA TTTCATCCCA GGAGCTAGGT CAGGGGCTTT CAGGGGGACT GCAGGCCATT 
GCAGAGTTAT TGCAGGTGGA CTGTGAGATG TATGGGCTTA CTAATGACCA CTACAGTGTT 
ACTTTAAGAC GGTATGCTGG AATGGCTTTG ACAAACTTGA CCTTTGGAGA TGTTGCCAAC 
AAGGCTACGC TGTGTTCTAT GAAAGGCTGC ATGAGAGCAC TTGTGGCCCA GTTAAAATCT 
GAGAGTGAAG ACTTACAGCA GGTTATTGCA AGTGTTTTGA GGAATTTGTC TTGGCGAGCA 
GATGTAAATA GCAAAAAGAC GTTGAGAGAA GTTGGAAGTG TGAAAGCATT GATGGAATGT 
GCTTTGGAAG TTAAAAAGGA ATCAACCCTC AAAAGCGTTT TGAGTGCCTT ATGGAACCTG 
TCTGCACACT GCACTGAGAA TAAGGCTGAC ATCTGTGCTG TGGATGGAGC ACTGGCATTT 
CTGGTTGGCA CCCTCACTTA CCGGAGCCAG ACAAATACTT TAGCCATTAT TGAAAGTGGA 
GGTGGGATAT TACGGAATGT GTCCAGCTTG ATAGCTACAA ACGAAGACCA CAGGCAAATC 
CTAAGAGAGA ACAATTGCCT ACAAACTTTA TTACAGCACT TGAAATCTCA CAGCTTGACA 
ATAGTCAGTA ATGCATGTGG AACTTTGTGG AATCTCTCAG CAAGAAATCC TAAAGACCAG 
GAAGCCTTGT GGGACATGGG GGCAGTGAGC ATGCTCAAGA ACCTCATTCA TTCCAAGCAC 
AAAATGATTG CCATGGGAAG TGCAGCAGCT TTAAGGAATC TCATGGCAAA CAGACCTGCA 
AAGTATAAGG ATGCCAATAT CATGTCTCCC GGCTCAAGTC TGCCATCCCT TCACGTTAGG 
AAACAGAAAG CTCTAGAAGC TGAGCTAGAT GCTCAGCATT TATCAGAAAC CTTCGACAAC 
ATTGACAACC TAAGTCCCAA GGCCTCTCAC CGGAGTAAGC AGAGACACAA GCAGAATCTT 
TATGGTGACT ATGCTTTTGA CGCCAATCGA CATGATGATA GTAGGTCAGA CAATTTCAAT 
ACTGGAAACA TGACTGTTCT TTCACCATAT TTAAATACTA CGGTATTGCC CAGCTCTTCT 
TCCTCAAGGG GAAGTTTAGA CAGTTCTCGT TCTGAGAAAG ACAGAAGTTT GGAGAGAGAG 
CGAGGTATTG GCCTCAGTGC TTACCATCCA ACAACAGAAA ATGCAGGAAC CTCATCAAAA 
CGAGGTCTGC AGATCACTAC CACTGCAGCC CAGATAGCCA AAGTTATGGA AGAAGTATCA 
GCCATTCATA CCTCCCAGGA CGACAGAAGT TCTGCTTCTA CCACCGAGTT CCATTGTGTG 
GCAGACGACA GGAGTGCGGC ACGAAGAAGC TCTGCCTCCC ACACACACTC AAACACATAC 
AACTTCACTA AGTCGGAAAA TTCAAATAGG ACATGCTCTA TGCCTTATGC CAAAGTGGAA 
TATAAACGAT CTTCAAATGA CAGTTTAAAT AGTGTCACTA GTAGTGATGG ATATGGTAAA 
AGAGGCCAAA TGAAACCCTC AGTTGAATCC TATTCTGAAG ATGATGAAAG TAAATTTTGC 
AGTTATGGTC AGTATCCAGC TGACCTAGCC CATAAGATAC ACAGTGCAAA TCATATGGAT 
GATAATGATG GAGAACTGGA TACACCAATA AATTACAGTC TTAAATATTC AGATGAGCAG 
TTGAACTCAG GAAGGCAGAG TCCCTCACAG AATGAAAGGT GGGCAAGACC AAAGCATGTG 
ATAGAAGATG AAATAAAGCA AAACGAGCAA AGACAAGCAA GAAGCCAGAA CACCAGTTAT 
CCTGTCTATT CTGAGAATAC CGATGACAAA CACCTCAAAT TCCAACCACA TTTTGGACAA 
CAAGAATGTG TTTCCCCATA TAGGTCAAGG GGAACCAGTG GTTCAGAAAC AAATCGAATG 
GGTTCTAGTC ATGCAATTAA TCAAAATGTA AACCAGTCTC TGTGTCAGGA AGATGATTAT 
GAAGATGATA AACCTACCAA CTACAGTGAA CGTTATTCTG AGGAAGAACA ACATGAAGAA 
GAAGAAGAGA GACCGACAAA TTATAGCATA AAATATAATG AAGAGAAACA TCATGTGGAT 
CAGCCTATTG ATTATAGTTT AAAATATGCC ACTGACATTT CTTCCTCACA AAAACCATCA 
TTTTCATTCT CAAAGAATTC ATCAGCACAA AGCACTAAAC CTGAACATCT CTCTCCAAGC 
AGCGAGAATA CAGCTGTACC TCCATCTAAT GCCAAAAGGC AGAATCAGCT GCGTCCAAGT 
TCAGCACAAA GAAATGGCCA GACTCAAAAA GGCACTACTT GCAAAGTCCC CTCCATCAAC 
CAAGAAACAA TACAGACTTA CTGCGTAGAA GACACCCCAA TATGTTTTTC AAGGTGCAGT 
TCATTATCAT CACTGTCATC AGCTGACGAT GAAATAGGAT GTGATCAGAC AACACAGGAA 
GCAGATTCTG CTAATACTCT GCAGACAGCA GAAGTAAAAG AGAATGATGT AACTCGGTCA 
GCTGAAGATC CTGCAACTGA AGTTCCAGCA GTGTCCCAGA ATGCTAGAGC CAAACCCAGC 
CGACTCCAGG CTTCTGGCTT ATCTTCAGAA TCAACCAGGC ATAATAAAGC TGTTGAGTTT 
TCTTCAGGAG CCAAGTCTCC CTCCAAAAGT GGTGCTCAGA CACCCAAAAG TCCCCCAGAA 
CACTATGTCC AGGAGACTCC GCTCGTATTC AGCAGGTGTA CTTCTGTCAG CTCCCTTGAC 
AGTTTTGAGA GTCGCTCCAT TGCCAGCTCT GTTCAGAGTG AGCCATGTAG TGGAATGGTG 
AGTGGCATCA TAAGCCCCAG TGACCTTCCA GATAGTCCTG GGCAGACCAT GCCACCAAGC 
AGAAGCAAAA CCCCTCCACC TCCTCCACAG ACAGTGCAGG CCAAGAGAGA GGTGCCAAAA 
AGTAAAGTCC CTGCTGCTGA GAAGAGAGAG AGTGGGCCTA AGCAGACTGC TGTAAATGCT 
GCCGTGCAGA GGGTGCAGGT CCTTCCAGAC GTGGATACTT TGTTACACTT CGCCACAGAA 
AGTACTCCAG ACGGGTTTTC TTGTTCCTCC AGCCTAAGTG CTCTGAGCCT GGATGAGCCA 
TTTATACAGA AAGATGTAGA ATTAAGAATC ATGCCTCCAG TTCAGGAAAA CGACAATGGG 
AATGAAACTG AATCAGAACA GCCTGAGGAA TCAAATGAAA ACCAGGATAA AGAGGTAGAA 
AAGCCTGACT CTGAAAAAGA CTTATTAGAT GATTCTGATG ACGATGATAT TGAAATATTA 
GAAGAATGTA TTATTTCAGC CATGCCAACA AAGTCATCAC GCAAAGCCAA AAAACTAGCC 
CAGACTGCTT CAAAATTACC TCCACCTGTG GCAAGGAAAC CAAGTCAGCT ACCTGTGTAT 
AAACTTCTGC CAGCACAGAA TAGGCTGCAG GCACAAAAAC ATGTTAGCTT TACACCAGGG 
GATGATGTGC CCCGGGTGTA CTGTGTAGAA GGGACACCTA TAAACTTTTC CACAGCAACG 
TCTCTAAGTG ATCTGACAAT AGAGTCCCCT CCAAATGAAT TGGCTACTGG AGATGGGGTC 
AGAGCGGGTA TACAGTCAGG TGAATTTGAA AAACGAGATA CCATTCCTAC AGAAGGCAGA 
AGTACAGATG ATGCTCAGCG AGGAAAAATC TCATCTATAG TTACACCAGA CCTGGATGAC 
AACAAAGCAG AGGAAGGAGA TATTCTTGCA GAATGTATCA ATTCTGCTAT GCCCAAAGGA 
AAAAGCCACA AGCCTTTCCG AGTGAAAAAG ATAATGGACC AAGTCCAACA AGCATCCTCG 
ACTTCATCTG GAGCTAACAA AAATCAAGTA GACACTAAGA AAAAGAAGCC TACTTCACCA 
GTAAAGCCCA TGCCACAAAA TACTGAATAT AGAACGCGTG TGAGAAAGAA TACAGACTCA 
AAAGTTAATG TAAATACTGA AGAAACTTTC TCAGACAACA AAGACTCAAA GAAACCAAGC 
TTACAAACCA ATGCCAAGGC CTTCAATGAA AAGCTACCTA ACAATGAAGA CAGAGTGCGG 
GGGAGCTTCG CCTTGGACTC ACCGCATCAC TACACCCCTA TTGAGGGGAC GCCGTACTGC 
TTTTCCCGAA ATGACTCCTT GAGTTCTCTG GATTTTGATG ATGACGATGT TGACCTTTCC 
AGGGAAAAGG CCGAGTTAAG AAAGGGCAAA GAAAGCAAGG ATTCCGAAGC CAAAGTTACC 
TGCCGCCCAG AACCAAACTC AAGCCAGCAG GCAGCTAGTA AGTCACAAGC CAGTATAAAA 
CATCCAGCAA ACAGAGCACA GTCCAAACCA GTGCTGCAGA AACAGCCCAC TTTCCCCCAG 
TCCTCCAAAG ACGGACCAGA TAGAGGGGCA GCAACTGACG AAAAACTGCA GAATTTTGCT 
ATTGAAAATA CTCCAGTTTG CTTTTCTCGA AATTCCTCTC TGAGTTCCCT TAGTGACATT 
GACCAGGAAA ACAACAATAA CAAAGAAAGT GAACCAATCA AAGAAGCTGA ACCTGCCAAC 
TCACAAGGAG AGCCCAGTAA GCCTCAGGCA TCCGGGTATG CTCCCAAGTC CTTCCACGTC 
GAAGACACCC CTGTCTGTTT CTCAAGAAAC AGCTCTCTCA GTTCTCTTAG CATTGACTCT 
GAGGACGACC TGTTACAGGA GTGTATAAGT TCTGCCATGC CAAAAAAGAA AAGGCCTTCA 
AGACTCAAGA GTGAGAGCGA AAAGCAGAGC CCTAGAAAAG TGGGTGGCAT ATTAGCTGAA 
GACCTGACGC TTGATTTGAA AGATCTACAG AGGCCAGATT CAGAACACGC TTTCTCCCCC 
GACTCAGAAA ATTTTGACTG GAAAGCTATT CAGGAAGGCG CAAACTCCAT AGTAAGTAGT 
TTGCACCAAG CTGCTGCAGC CGCCGCGTGC TTATCTAGAC AAGCGTCATC CGACTCAGAT 
TCCATTCTGT CACTAAAGTC CGGCATTTCT CTGGGATCGC CTTTTCATCT TACACCTGAT 
CAAGAGGAAA AGCCATTCAC AAGCAATAAA GGCCCAAGAA TTCTCAAACC TGGAGAGAAA 
AGCACATTAG AAGCAAAAAA AATAGAATCT GAAAACAAAG GAATCAAAGG CGGGAAAAAG 
GTTTATAAAA GCTTGATTAC GGGAAAGATT CGCTCCAATT CAGAAATTTC CAGCCAAATG 
AAACAACCCC TCCCGACAAA CATGCCTTCA ATCTCAAGAG GCAGGACGAT GATTCACATC 
CCAGGGCTTC GGAATAGCTC CTCTAGTACA AGCCCTGTCT CTAAGAAAGG CCCACCCCTC 
AAGACTCCAG CCTCTAAAAG CCCCAGTGAA GGGCCGGGAG CTACCACTTC TCCTCGAGGA 
ACTAAGCCAG CAGGAAAGTC AGAGCTTAGC CCTATCACCA GGCAAACTTC CCAAATCAGT 
GGGTCAAATA AGGGGTCTTC TAGATCAGGA TCTAGAGACT CCACTCCCTC AAGACCTACA 
CAGCAACCAT TAAGTAGGCC AATGCAGTCT CCAGGGCGAA ACTCAATTTC CCCTGGTAGA 
AATGGAATAA GCCCTCCTAA CAAACTGTCT CAGCTGCCCA GAACATCATC TCCCAGTACT 
GCTTCAACTA AGTCCTCCGG TTCTGGGAAA ATGTCATATA CATCCCCAGG TAGACAGCTG 
AGCCAACAAA ATCTTACCAA ACAAGCAAGT TTATCCAAGA ATGCCAGCAG TATCCCCAGA 
AGTGAGTCGG CATCTAAAGG ACTGAATCAG ATGAGTAACG GCAATGGGTC AAATAAAAAG 
GTAGAACTTT CTAGAATGTC TTCAACTAAA TCAAGTGGAA GTGAATCAGA CAGATCAGAA 
AGGCCTGCAT TAGTACGCCA GTCTACTTTC ATCAAAGAAG CCCCAAGCCC AACCCTGAGG 
AGGAAACTGG AGGAATCTGC CTCATTTGAA TCCCTTTCTC CATCTTCTAG ACCAGATTCT 
CCCACCAGGT CGCAGGCACA GACCCCAGTT TTAAGCCCTT CCCTTCCTGA TATGTCTCTG 
TCCACACATC CATCTGTTCA GGCAGGTGGG TGGCGAAAGC TCCCGCCTAA TCTCAGCCCC 
ACTATCGAGT ATAATGACGG AAGGCCCACA AAACGGCATG ATATTGCACG CTCCCATTCT 
GAAAGTCCTT CCAGACTACC AATCAACCGG GCGGGAACCT GGAAGCGTGA ACACAGCAAA 
CATTCCTCGT CCCTTCCTCG AGTGAGTACT TGGAGAAGAA CTGGAAGCTC ATCTTCTATT 
CTTTCTGCTT CATCAGAGTC CAGTGAAAAA GCAAAAAGTG AGGATGAAAG GCATGTGAGC 
TCCATGCCAG CACCCAGACA GATGAAGGAA AACCAGGTGC CCACCAAAGG AACATGGAGG 
AAAATCAAGG AAAGTGACAT TTCTCCCACA GGCATGGCTT CTCAGAGCGC TTCCTCAGGT 
GCTGCCAGTG GTGCTGAATC CAAGCCTCTG ATCTATCAGA TGGCACCTCC TGTCTCTAAA 
ACAGAGGATG TTTGGGTGAG AATTGAGGAC TGCCCCATTA ACAACCCTAG ATCTGGACGG 
TCCCCCACAG GCAACACCCC CCCAGTGATT GACAGTGTTT CAGAGAAGGG AAGTTCAAGC 
ATTAAAGATT CAAAAGACAC CCATGGGAAA CAGAGTGTGG GCAGTGGCAG TCCTGTGCAA 
ACCGTGGGTC TGGAAACCCG CCTCAACTCC TTTGTTCAGG TAGAGGCCCC AGAACAGAAA 
GGAACTGAGG CAAAACCAGG ACAGAGTAAC CCAGTCTCTA TAGCAGAGAC TGCTGAGACG 
TGTATAGCAG AGCGTACCCC TTTCAGTTCC AGTAGCTCCA GCAAGCACAG CTCACCTAGC 
GGGACTGTTG CTGCCAGAGT GACACCTTTT AATTACAACC CTAGCCCTAG GAAGAGCAGC 
GCAGACAGCA CTTCAGCCCG GCCGTCTCAG ATCCCTACGC CAGTGAGCAC CAACACGAAG 
AAGAGAGATT CGAAGACTGA CAGCACAGAA TCCAGTGGAG CCCAAAGTCC TAAACGCCAT 
TCCGGGTCTT ACCTCGTGAC GTCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525538.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525601.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X18, mRNA.

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CloneID	OMu54323
Clone ID Related Accession (Same CDS sequence)	XM_006525538.3 , XM_006525538.4
Accession Version	XM_006525538.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525538.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGATA 
CGAGCTTACT GTGAAACCTG TTGGGAGTGG CAGGAAGCCC ACGAACAAGG CATGGACCAG 
GACAAAAACC CAATGCCAGC TCCTGTTGAG CATCAGATCT GTCCTGCTGT GTGTGTTCTA 
ATGAAGCTTT CATTTGATGA AGAGCATAGG CATGCAATGA ATGAACTTGG TAGGAAGGCT 
ACCCGGGGCA TTTCATCCCA GGAGCTAGGT CAGGGGCTTT CAGGGGGACT GCAGGCCATT 
GCAGAGTTAT TGCAGGTGGA CTGTGAGATG TATGGGCTTA CTAATGACCA CTACAGTGTT 
ACTTTAAGAC GGTATGCTGG AATGGCTTTG ACAAACTTGA CCTTTGGAGA TGTTGCCAAC 
AAGGCTACGC TGTGTTCTAT GAAAGGCTGC ATGAGAGCAC TTGTGGCCCA GTTAAAATCT 
GAGAGTGAAG ACTTACAGCA GGTTATTGCA AGTGTTTTGA GGAATTTGTC TTGGCGAGCA 
GATGTAAATA GCAAAAAGAC GTTGAGAGAA GTTGGAAGTG TGAAAGCATT GATGGAATGT 
GCTTTGGAAG TTAAAAAGGA ATCAACCCTC AAAAGCGTTT TGAGTGCCTT ATGGAACCTG 
TCTGCACACT GCACTGAGAA TAAGGCTGAC ATCTGTGCTG TGGATGGAGC ACTGGCATTT 
CTGGTTGGCA CCCTCACTTA CCGGAGCCAG ACAAATACTT TAGCCATTAT TGAAAGTGGA 
GGTGGGATAT TACGGAATGT GTCCAGCTTG ATAGCTACAA ACGAAGACCA CAGGCAAATC 
CTAAGAGAGA ACAATTGCCT ACAAACTTTA TTACAGCACT TGAAATCTCA CAGCTTGACA 
ATAGTCAGTA ATGCATGTGG AACTTTGTGG AATCTCTCAG CAAGAAATCC TAAAGACCAG 
GAAGCCTTGT GGGACATGGG GGCAGTGAGC ATGCTCAAGA ACCTCATTCA TTCCAAGCAC 
AAAATGATTG CCATGGGAAG TGCAGCAGCT TTAAGGAATC TCATGGCAAA CAGACCTGCA 
AAGTATAAGG ATGCCAATAT CATGTCTCCC GGCTCAAGTC TGCCATCCCT TCACGTTAGG 
AAACAGAAAG CTCTAGAAGC TGAGCTAGAT GCTCAGCATT TATCAGAAAC CTTCGACAAC 
ATTGACAACC TAAGTCCCAA GGCCTCTCAC CGGAGTAAGC AGAGACACAA GCAGAATCTT 
TATGGTGACT ATGCTTTTGA CGCCAATCGA CATGATGATA GTAGGTCAGA CAATTTCAAT 
ACTGGAAACA TGACTGTTCT TTCACCATAT TTAAATACTA CGGTATTGCC CAGCTCTTCT 
TCCTCAAGGG GAAGTTTAGA CAGTTCTCGT TCTGAGAAAG ACAGAAGTTT GGAGAGAGAG 
CGAGGTATTG GCCTCAGTGC TTACCATCCA ACAACAGAAA ATGCAGGAAC CTCATCAAAA 
CGAGGTCTGC AGATCACTAC CACTGCAGCC CAGATAGCCA AAGTTATGGA AGAAGTATCA 
GCCATTCATA CCTCCCAGGA CGACAGAAGT TCTGCTTCTA CCACCGAGTT CCATTGTGTG 
GCAGACGACA GGAGTGCGGC ACGAAGAAGC TCTGCCTCCC ACACACACTC AAACACATAC 
AACTTCACTA AGTCGGAAAA TTCAAATAGG ACATGCTCTA TGCCTTATGC CAAAGTGGAA 
TATAAACGAT CTTCAAATGA CAGTTTAAAT AGTGTCACTA GTAGTGATGG ATATGGTAAA 
AGAGGCCAAA TGAAACCCTC AGTTGAATCC TATTCTGAAG ATGATGAAAG TAAATTTTGC 
AGTTATGGTC AGTATCCAGC TGACCTAGCC CATAAGATAC ACAGTGCAAA TCATATGGAT 
GATAATGATG GAGAACTGGA TACACCAATA AATTACAGTC TTAAATATTC AGATGAGCAG 
TTGAACTCAG GAAGGCAGAG TCCCTCACAG AATGAAAGGT GGGCAAGACC AAAGCATGTG 
ATAGAAGATG AAATAAAGCA AAACGAGCAA AGACAAGCAA GAAGCCAGAA CACCAGTTAT 
CCTGTCTATT CTGAGAATAC CGATGACAAA CACCTCAAAT TCCAACCACA TTTTGGACAA 
CAAGAATGTG TTTCCCCATA TAGGTCAAGG GGAACCAGTG GTTCAGAAAC AAATCGAATG 
GGTTCTAGTC ATGCAATTAA TCAAAATGTA AACCAGTCTC TGTGTCAGGA AGATGATTAT 
GAAGATGATA AACCTACCAA CTACAGTGAA CGTTATTCTG AGGAAGAACA ACATGAAGAA 
GAAGAAGAGA GACCGACAAA TTATAGCATA AAATATAATG AAGAGAAACA TCATGTGGAT 
CAGCCTATTG ATTATAGTTT AAAATATGCC ACTGACATTT CTTCCTCACA AAAACCATCA 
TTTTCATTCT CAAAGAATTC ATCAGCACAA AGCACTAAAC CTGAACATCT CTCTCCAAGC 
AGCGAGAATA CAGCTGTACC TCCATCTAAT GCCAAAAGGC AGAATCAGCT GCGTCCAAGT 
TCAGCACAAA GAAATGGCCA GACTCAAAAA GGCACTACTT GCAAAGTCCC CTCCATCAAC 
CAAGAAACAA TACAGACTTA CTGCGTAGAA GACACCCCAA TATGTTTTTC AAGGTGCAGT 
TCATTATCAT CACTGTCATC AGCTGACGAT GAAATAGGAT GTGATCAGAC AACACAGGAA 
GCAGATTCTG CTAATACTCT GCAGACAGCA GAAGTAAAAG AGAATGATGT AACTCGGTCA 
GCTGAAGATC CTGCAACTGA AGTTCCAGCA GTGTCCCAGA ATGCTAGAGC CAAACCCAGC 
CGACTCCAGG CTTCTGGCTT ATCTTCAGAA TCAACCAGGC ATAATAAAGC TGTTGAGTTT 
TCTTCAGGAG CCAAGTCTCC CTCCAAAAGT GGTGCTCAGA CACCCAAAAG TCCCCCAGAA 
CACTATGTCC AGGAGACTCC GCTCGTATTC AGCAGGTGTA CTTCTGTCAG CTCCCTTGAC 
AGTTTTGAGA GTCGCTCCAT TGCCAGCTCT GTTCAGAGTG AGCCATGTAG TGGAATGGTG 
AGTGGCATCA TAAGCCCCAG TGACCTTCCA GATAGTCCTG GGCAGACCAT GCCACCAAGC 
AGAAGCAAAA CCCCTCCACC TCCTCCACAG ACAGTGCAGG CCAAGAGAGA GGTGCCAAAA 
AGTAAAGTCC CTGCTGCTGA GAAGAGAGAG AGTGGGCCTA AGCAGACTGC TGTAAATGCT 
GCCGTGCAGA GGGTGCAGGT CCTTCCAGAC GTGGATACTT TGTTACACTT CGCCACAGAA 
AGTACTCCAG ACGGGTTTTC TTGTTCCTCC AGCCTAAGTG CTCTGAGCCT GGATGAGCCA 
TTTATACAGA AAGATGTAGA ATTAAGAATC ATGCCTCCAG TTCAGGAAAA CGACAATGGG 
AATGAAACTG AATCAGAACA GCCTGAGGAA TCAAATGAAA ACCAGGATAA AGAGGTAGAA 
AAGCCTGACT CTGAAAAAGA CTTATTAGAT GATTCTGATG ACGATGATAT TGAAATATTA 
GAAGAATGTA TTATTTCAGC CATGCCAACA AAGTCATCAC GCAAAGCCAA AAAACTAGCC 
CAGACTGCTT CAAAATTACC TCCACCTGTG GCAAGGAAAC CAAGTCAGCT ACCTGTGTAT 
AAACTTCTGC CAGCACAGAA TAGGCTGCAG GCACAAAAAC ATGTTAGCTT TACACCAGGG 
GATGATGTGC CCCGGGTGTA CTGTGTAGAA GGGACACCTA TAAACTTTTC CACAGCAACG 
TCTCTAAGTG ATCTGACAAT AGAGTCCCCT CCAAATGAAT TGGCTACTGG AGATGGGGTC 
AGAGCGGGTA TACAGTCAGG TGAATTTGAA AAACGAGATA CCATTCCTAC AGAAGGCAGA 
AGTACAGATG ATGCTCAGCG AGGAAAAATC TCATCTATAG TTACACCAGA CCTGGATGAC 
AACAAAGCAG AGGAAGGAGA TATTCTTGCA GAATGTATCA ATTCTGCTAT GCCCAAAGGA 
AAAAGCCACA AGCCTTTCCG AGTGAAAAAG ATAATGGACC AAGTCCAACA AGCATCCTCG 
ACTTCATCTG GAGCTAACAA AAATCAAGTA GACACTAAGA AAAAGAAGCC TACTTCACCA 
GTAAAGCCCA TGCCACAAAA TACTGAATAT AGAACGCGTG TGAGAAAGAA TACAGACTCA 
AAAGTTAATG TAAATACTGA AGAAACTTTC TCAGACAACA AAGACTCAAA GAAACCAAGC 
TTACAAACCA ATGCCAAGGC CTTCAATGAA AAGCTACCTA ACAATGAAGA CAGAGTGCGG 
GGGAGCTTCG CCTTGGACTC ACCGCATCAC TACACCCCTA TTGAGGGGAC GCCGTACTGC 
TTTTCCCGAA ATGACTCCTT GAGTTCTCTG GATTTTGATG ATGACGATGT TGACCTTTCC 
AGGGAAAAGG CCGAGTTAAG AAAGGGCAAA GAAAGCAAGG ATTCCGAAGC CAAAGTTACC 
TGCCGCCCAG AACCAAACTC AAGCCAGCAG GCAGCTAGTA AGTCACAAGC CAGTATAAAA 
CATCCAGCAA ACAGAGCACA GTCCAAACCA GTGCTGCAGA AACAGCCCAC TTTCCCCCAG 
TCCTCCAAAG ACGGACCAGA TAGAGGGGCA GCAACTGACG AAAAACTGCA GAATTTTGCT 
ATTGAAAATA CTCCAGTTTG CTTTTCTCGA AATTCCTCTC TGAGTTCCCT TAGTGACATT 
GACCAGGAAA ACAACAATAA CAAAGAAAGT GAACCAATCA AAGAAGCTGA ACCTGCCAAC 
TCACAAGGAG AGCCCAGTAA GCCTCAGGCA TCCGGGTATG CTCCCAAGTC CTTCCACGTC 
GAAGACACCC CTGTCTGTTT CTCAAGAAAC AGCTCTCTCA GTTCTCTTAG CATTGACTCT 
GAGGACGACC TGTTACAGGA GTGTATAAGT TCTGCCATGC CAAAAAAGAA AAGGCCTTCA 
AGACTCAAGA GTGAGAGCGA AAAGCAGAGC CCTAGAAAAG TGGGTGGCAT ATTAGCTGAA 
GACCTGACGC TTGATTTGAA AGATCTACAG AGGCCAGATT CAGAACACGC TTTCTCCCCC 
GACTCAGAAA ATTTTGACTG GAAAGCTATT CAGGAAGGCG CAAACTCCAT AGTAAGTAGT 
TTGCACCAAG CTGCTGCAGC CGCCGCGTGC TTATCTAGAC AAGCGTCATC CGACTCAGAT 
TCCATTCTGT CACTAAAGTC CGGCATTTCT CTGGGATCGC CTTTTCATCT TACACCTGAT 
CAAGAGGAAA AGCCATTCAC AAGCAATAAA GGCCCAAGAA TTCTCAAACC TGGAGAGAAA 
AGCACATTAG AAGCAAAAAA AATAGAATCT GAAAACAAAG GAATCAAAGG CGGGAAAAAG 
GTTTATAAAA GCTTGATTAC GGGAAAGATT CGCTCCAATT CAGAAATTTC CAGCCAAATG 
AAACAACCCC TCCCGACAAA CATGCCTTCA ATCTCAAGAG GCAGGACGAT GATTCACATC 
CCAGGGCTTC GGAATAGCTC CTCTAGTACA AGCCCTGTCT CTAAGAAAGG CCCACCCCTC 
AAGACTCCAG CCTCTAAAAG CCCCAGTGAA GGGCCGGGAG CTACCACTTC TCCTCGAGGA 
ACTAAGCCAG CAGGAAAGTC AGAGCTTAGC CCTATCACCA GGCAAACTTC CCAAATCAGT 
GGGTCAAATA AGGGGTCTTC TAGATCAGGA TCTAGAGACT CCACTCCCTC AAGACCTACA 
CAGCAACCAT TAAGTAGGCC AATGCAGTCT CCAGGGCGAA ACTCAATTTC CCCTGGTAGA 
AATGGAATAA GCCCTCCTAA CAAACTGTCT CAGCTGCCCA GAACATCATC TCCCAGTACT 
GCTTCAACTA AGTCCTCCGG TTCTGGGAAA ATGTCATATA CATCCCCAGG TAGACAGCTG 
AGCCAACAAA ATCTTACCAA ACAAGCAAGT TTATCCAAGA ATGCCAGCAG TATCCCCAGA 
AGTGAGTCGG CATCTAAAGG ACTGAATCAG ATGAGTAACG GCAATGGGTC AAATAAAAAG 
GTAGAACTTT CTAGAATGTC TTCAACTAAA TCAAGTGGAA GTGAATCAGA CAGATCAGAA 
AGGCCTGCAT TAGTACGCCA GTCTACTTTC ATCAAAGAAG CCCCAAGCCC AACCCTGAGG 
AGGAAACTGG AGGAATCTGC CTCATTTGAA TCCCTTTCTC CATCTTCTAG ACCAGATTCT 
CCCACCAGGT CGCAGGCACA GACCCCAGTT TTAAGCCCTT CCCTTCCTGA TATGTCTCTG 
TCCACACATC CATCTGTTCA GGCAGGTGGG TGGCGAAAGC TCCCGCCTAA TCTCAGCCCC 
ACTATCGAGT ATAATGACGG AAGGCCCACA AAACGGCATG ATATTGCACG CTCCCATTCT 
GAAAGTCCTT CCAGACTACC AATCAACCGG GCGGGAACCT GGAAGCGTGA ACACAGCAAA 
CATTCCTCGT CCCTTCCTCG AGTGAGTACT TGGAGAAGAA CTGGAAGCTC ATCTTCTATT 
CTTTCTGCTT CATCAGAGTC CAGTGAAAAA GCAAAAAGTG AGGATGAAAG GCATGTGAGC 
TCCATGCCAG CACCCAGACA GATGAAGGAA AACCAGGTGC CCACCAAAGG AACATGGAGG 
AAAATCAAGG AAAGTGACAT TTCTCCCACA GGCATGGCTT CTCAGAGCGC TTCCTCAGGT 
GCTGCCAGTG GTGCTGAATC CAAGCCTCTG ATCTATCAGA TGGCACCTCC TGTCTCTAAA 
ACAGAGGATG TTTGGGTGAG AATTGAGGAC TGCCCCATTA ACAACCCTAG ATCTGGACGG 
TCCCCCACAG GCAACACCCC CCCAGTGATT GACAGTGTTT CAGAGAAGGG AAGTTCAAGC 
ATTAAAGATT CAAAAGACAC CCATGGGAAA CAGAGTGTGG GCAGTGGCAG TCCTGTGCAA 
ACCGTGGGTC TGGAAACCCG CCTCAACTCC TTTGTTCAGG TAGAGGCCCC AGAACAGAAA 
GGAACTGAGG CAAAACCAGG ACAGAGTAAC CCAGTCTCTA TAGCAGAGAC TGCTGAGACG 
TGTATAGCAG AGCGTACCCC TTTCAGTTCC AGTAGCTCCA GCAAGCACAG CTCACCTAGC 
GGGACTGTTG CTGCCAGAGT GACACCTTTT AATTACAACC CTAGCCCTAG GAAGAGCAGC 
GCAGACAGCA CTTCAGCCCG GCCGTCTCAG ATCCCTACGC CAGTGAGCAC CAACACGAAG 
AAGAGAGATT CGAAGACTGA CAGCACAGAA TCCAGTGGAG CCCAAAGTCC TAAACGCCAT 
TCCGGGTCTT ACCTCGTGAC GTCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525601.1
CDS	816..9125
Translation	MYAFLRSGPVAALPASVPPVTLGARSSGGGRGRVPGEGRRPGGARACGRGASVWQEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525538.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525538.4

ORF Insert Sequence:

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGATA 
CGAGCTTACT GTGAAACCTG TTGGGAGTGG CAGGAAGCCC ACGAACAAGG CATGGACCAG 
GACAAAAACC CAATGCCAGC TCCTGTTGAG CATCAGATCT GTCCTGCTGT GTGTGTTCTA 
ATGAAGCTTT CATTTGATGA AGAGCATAGG CATGCAATGA ATGAACTTGG TAGGAAGGCT 
ACCCGGGGCA TTTCATCCCA GGAGCTAGGT CAGGGGCTTT CAGGGGGACT GCAGGCCATT 
GCAGAGTTAT TGCAGGTGGA CTGTGAGATG TATGGGCTTA CTAATGACCA CTACAGTGTT 
ACTTTAAGAC GGTATGCTGG AATGGCTTTG ACAAACTTGA CCTTTGGAGA TGTTGCCAAC 
AAGGCTACGC TGTGTTCTAT GAAAGGCTGC ATGAGAGCAC TTGTGGCCCA GTTAAAATCT 
GAGAGTGAAG ACTTACAGCA GGTTATTGCA AGTGTTTTGA GGAATTTGTC TTGGCGAGCA 
GATGTAAATA GCAAAAAGAC GTTGAGAGAA GTTGGAAGTG TGAAAGCATT GATGGAATGT 
GCTTTGGAAG TTAAAAAGGA ATCAACCCTC AAAAGCGTTT TGAGTGCCTT ATGGAACCTG 
TCTGCACACT GCACTGAGAA TAAGGCTGAC ATCTGTGCTG TGGATGGAGC ACTGGCATTT 
CTGGTTGGCA CCCTCACTTA CCGGAGCCAG ACAAATACTT TAGCCATTAT TGAAAGTGGA 
GGTGGGATAT TACGGAATGT GTCCAGCTTG ATAGCTACAA ACGAAGACCA CAGGCAAATC 
CTAAGAGAGA ACAATTGCCT ACAAACTTTA TTACAGCACT TGAAATCTCA CAGCTTGACA 
ATAGTCAGTA ATGCATGTGG AACTTTGTGG AATCTCTCAG CAAGAAATCC TAAAGACCAG 
GAAGCCTTGT GGGACATGGG GGCAGTGAGC ATGCTCAAGA ACCTCATTCA TTCCAAGCAC 
AAAATGATTG CCATGGGAAG TGCAGCAGCT TTAAGGAATC TCATGGCAAA CAGACCTGCA 
AAGTATAAGG ATGCCAATAT CATGTCTCCC GGCTCAAGTC TGCCATCCCT TCACGTTAGG 
AAACAGAAAG CTCTAGAAGC TGAGCTAGAT GCTCAGCATT TATCAGAAAC CTTCGACAAC 
ATTGACAACC TAAGTCCCAA GGCCTCTCAC CGGAGTAAGC AGAGACACAA GCAGAATCTT 
TATGGTGACT ATGCTTTTGA CGCCAATCGA CATGATGATA GTAGGTCAGA CAATTTCAAT 
ACTGGAAACA TGACTGTTCT TTCACCATAT TTAAATACTA CGGTATTGCC CAGCTCTTCT 
TCCTCAAGGG GAAGTTTAGA CAGTTCTCGT TCTGAGAAAG ACAGAAGTTT GGAGAGAGAG 
CGAGGTATTG GCCTCAGTGC TTACCATCCA ACAACAGAAA ATGCAGGAAC CTCATCAAAA 
CGAGGTCTGC AGATCACTAC CACTGCAGCC CAGATAGCCA AAGTTATGGA AGAAGTATCA 
GCCATTCATA CCTCCCAGGA CGACAGAAGT TCTGCTTCTA CCACCGAGTT CCATTGTGTG 
GCAGACGACA GGAGTGCGGC ACGAAGAAGC TCTGCCTCCC ACACACACTC AAACACATAC 
AACTTCACTA AGTCGGAAAA TTCAAATAGG ACATGCTCTA TGCCTTATGC CAAAGTGGAA 
TATAAACGAT CTTCAAATGA CAGTTTAAAT AGTGTCACTA GTAGTGATGG ATATGGTAAA 
AGAGGCCAAA TGAAACCCTC AGTTGAATCC TATTCTGAAG ATGATGAAAG TAAATTTTGC 
AGTTATGGTC AGTATCCAGC TGACCTAGCC CATAAGATAC ACAGTGCAAA TCATATGGAT 
GATAATGATG GAGAACTGGA TACACCAATA AATTACAGTC TTAAATATTC AGATGAGCAG 
TTGAACTCAG GAAGGCAGAG TCCCTCACAG AATGAAAGGT GGGCAAGACC AAAGCATGTG 
ATAGAAGATG AAATAAAGCA AAACGAGCAA AGACAAGCAA GAAGCCAGAA CACCAGTTAT 
CCTGTCTATT CTGAGAATAC CGATGACAAA CACCTCAAAT TCCAACCACA TTTTGGACAA 
CAAGAATGTG TTTCCCCATA TAGGTCAAGG GGAACCAGTG GTTCAGAAAC AAATCGAATG 
GGTTCTAGTC ATGCAATTAA TCAAAATGTA AACCAGTCTC TGTGTCAGGA AGATGATTAT 
GAAGATGATA AACCTACCAA CTACAGTGAA CGTTATTCTG AGGAAGAACA ACATGAAGAA 
GAAGAAGAGA GACCGACAAA TTATAGCATA AAATATAATG AAGAGAAACA TCATGTGGAT 
CAGCCTATTG ATTATAGTTT AAAATATGCC ACTGACATTT CTTCCTCACA AAAACCATCA 
TTTTCATTCT CAAAGAATTC ATCAGCACAA AGCACTAAAC CTGAACATCT CTCTCCAAGC 
AGCGAGAATA CAGCTGTACC TCCATCTAAT GCCAAAAGGC AGAATCAGCT GCGTCCAAGT 
TCAGCACAAA GAAATGGCCA GACTCAAAAA GGCACTACTT GCAAAGTCCC CTCCATCAAC 
CAAGAAACAA TACAGACTTA CTGCGTAGAA GACACCCCAA TATGTTTTTC AAGGTGCAGT 
TCATTATCAT CACTGTCATC AGCTGACGAT GAAATAGGAT GTGATCAGAC AACACAGGAA 
GCAGATTCTG CTAATACTCT GCAGACAGCA GAAGTAAAAG AGAATGATGT AACTCGGTCA 
GCTGAAGATC CTGCAACTGA AGTTCCAGCA GTGTCCCAGA ATGCTAGAGC CAAACCCAGC 
CGACTCCAGG CTTCTGGCTT ATCTTCAGAA TCAACCAGGC ATAATAAAGC TGTTGAGTTT 
TCTTCAGGAG CCAAGTCTCC CTCCAAAAGT GGTGCTCAGA CACCCAAAAG TCCCCCAGAA 
CACTATGTCC AGGAGACTCC GCTCGTATTC AGCAGGTGTA CTTCTGTCAG CTCCCTTGAC 
AGTTTTGAGA GTCGCTCCAT TGCCAGCTCT GTTCAGAGTG AGCCATGTAG TGGAATGGTG 
AGTGGCATCA TAAGCCCCAG TGACCTTCCA GATAGTCCTG GGCAGACCAT GCCACCAAGC 
AGAAGCAAAA CCCCTCCACC TCCTCCACAG ACAGTGCAGG CCAAGAGAGA GGTGCCAAAA 
AGTAAAGTCC CTGCTGCTGA GAAGAGAGAG AGTGGGCCTA AGCAGACTGC TGTAAATGCT 
GCCGTGCAGA GGGTGCAGGT CCTTCCAGAC GTGGATACTT TGTTACACTT CGCCACAGAA 
AGTACTCCAG ACGGGTTTTC TTGTTCCTCC AGCCTAAGTG CTCTGAGCCT GGATGAGCCA 
TTTATACAGA AAGATGTAGA ATTAAGAATC ATGCCTCCAG TTCAGGAAAA CGACAATGGG 
AATGAAACTG AATCAGAACA GCCTGAGGAA TCAAATGAAA ACCAGGATAA AGAGGTAGAA 
AAGCCTGACT CTGAAAAAGA CTTATTAGAT GATTCTGATG ACGATGATAT TGAAATATTA 
GAAGAATGTA TTATTTCAGC CATGCCAACA AAGTCATCAC GCAAAGCCAA AAAACTAGCC 
CAGACTGCTT CAAAATTACC TCCACCTGTG GCAAGGAAAC CAAGTCAGCT ACCTGTGTAT 
AAACTTCTGC CAGCACAGAA TAGGCTGCAG GCACAAAAAC ATGTTAGCTT TACACCAGGG 
GATGATGTGC CCCGGGTGTA CTGTGTAGAA GGGACACCTA TAAACTTTTC CACAGCAACG 
TCTCTAAGTG ATCTGACAAT AGAGTCCCCT CCAAATGAAT TGGCTACTGG AGATGGGGTC 
AGAGCGGGTA TACAGTCAGG TGAATTTGAA AAACGAGATA CCATTCCTAC AGAAGGCAGA 
AGTACAGATG ATGCTCAGCG AGGAAAAATC TCATCTATAG TTACACCAGA CCTGGATGAC 
AACAAAGCAG AGGAAGGAGA TATTCTTGCA GAATGTATCA ATTCTGCTAT GCCCAAAGGA 
AAAAGCCACA AGCCTTTCCG AGTGAAAAAG ATAATGGACC AAGTCCAACA AGCATCCTCG 
ACTTCATCTG GAGCTAACAA AAATCAAGTA GACACTAAGA AAAAGAAGCC TACTTCACCA 
GTAAAGCCCA TGCCACAAAA TACTGAATAT AGAACGCGTG TGAGAAAGAA TACAGACTCA 
AAAGTTAATG TAAATACTGA AGAAACTTTC TCAGACAACA AAGACTCAAA GAAACCAAGC 
TTACAAACCA ATGCCAAGGC CTTCAATGAA AAGCTACCTA ACAATGAAGA CAGAGTGCGG 
GGGAGCTTCG CCTTGGACTC ACCGCATCAC TACACCCCTA TTGAGGGGAC GCCGTACTGC 
TTTTCCCGAA ATGACTCCTT GAGTTCTCTG GATTTTGATG ATGACGATGT TGACCTTTCC 
AGGGAAAAGG CCGAGTTAAG AAAGGGCAAA GAAAGCAAGG ATTCCGAAGC CAAAGTTACC 
TGCCGCCCAG AACCAAACTC AAGCCAGCAG GCAGCTAGTA AGTCACAAGC CAGTATAAAA 
CATCCAGCAA ACAGAGCACA GTCCAAACCA GTGCTGCAGA AACAGCCCAC TTTCCCCCAG 
TCCTCCAAAG ACGGACCAGA TAGAGGGGCA GCAACTGACG AAAAACTGCA GAATTTTGCT 
ATTGAAAATA CTCCAGTTTG CTTTTCTCGA AATTCCTCTC TGAGTTCCCT TAGTGACATT 
GACCAGGAAA ACAACAATAA CAAAGAAAGT GAACCAATCA AAGAAGCTGA ACCTGCCAAC 
TCACAAGGAG AGCCCAGTAA GCCTCAGGCA TCCGGGTATG CTCCCAAGTC CTTCCACGTC 
GAAGACACCC CTGTCTGTTT CTCAAGAAAC AGCTCTCTCA GTTCTCTTAG CATTGACTCT 
GAGGACGACC TGTTACAGGA GTGTATAAGT TCTGCCATGC CAAAAAAGAA AAGGCCTTCA 
AGACTCAAGA GTGAGAGCGA AAAGCAGAGC CCTAGAAAAG TGGGTGGCAT ATTAGCTGAA 
GACCTGACGC TTGATTTGAA AGATCTACAG AGGCCAGATT CAGAACACGC TTTCTCCCCC 
GACTCAGAAA ATTTTGACTG GAAAGCTATT CAGGAAGGCG CAAACTCCAT AGTAAGTAGT 
TTGCACCAAG CTGCTGCAGC CGCCGCGTGC TTATCTAGAC AAGCGTCATC CGACTCAGAT 
TCCATTCTGT CACTAAAGTC CGGCATTTCT CTGGGATCGC CTTTTCATCT TACACCTGAT 
CAAGAGGAAA AGCCATTCAC AAGCAATAAA GGCCCAAGAA TTCTCAAACC TGGAGAGAAA 
AGCACATTAG AAGCAAAAAA AATAGAATCT GAAAACAAAG GAATCAAAGG CGGGAAAAAG 
GTTTATAAAA GCTTGATTAC GGGAAAGATT CGCTCCAATT CAGAAATTTC CAGCCAAATG 
AAACAACCCC TCCCGACAAA CATGCCTTCA ATCTCAAGAG GCAGGACGAT GATTCACATC 
CCAGGGCTTC GGAATAGCTC CTCTAGTACA AGCCCTGTCT CTAAGAAAGG CCCACCCCTC 
AAGACTCCAG CCTCTAAAAG CCCCAGTGAA GGGCCGGGAG CTACCACTTC TCCTCGAGGA 
ACTAAGCCAG CAGGAAAGTC AGAGCTTAGC CCTATCACCA GGCAAACTTC CCAAATCAGT 
GGGTCAAATA AGGGGTCTTC TAGATCAGGA TCTAGAGACT CCACTCCCTC AAGACCTACA 
CAGCAACCAT TAAGTAGGCC AATGCAGTCT CCAGGGCGAA ACTCAATTTC CCCTGGTAGA 
AATGGAATAA GCCCTCCTAA CAAACTGTCT CAGCTGCCCA GAACATCATC TCCCAGTACT 
GCTTCAACTA AGTCCTCCGG TTCTGGGAAA ATGTCATATA CATCCCCAGG TAGACAGCTG 
AGCCAACAAA ATCTTACCAA ACAAGCAAGT TTATCCAAGA ATGCCAGCAG TATCCCCAGA 
AGTGAGTCGG CATCTAAAGG ACTGAATCAG ATGAGTAACG GCAATGGGTC AAATAAAAAG 
GTAGAACTTT CTAGAATGTC TTCAACTAAA TCAAGTGGAA GTGAATCAGA CAGATCAGAA 
AGGCCTGCAT TAGTACGCCA GTCTACTTTC ATCAAAGAAG CCCCAAGCCC AACCCTGAGG 
AGGAAACTGG AGGAATCTGC CTCATTTGAA TCCCTTTCTC CATCTTCTAG ACCAGATTCT 
CCCACCAGGT CGCAGGCACA GACCCCAGTT TTAAGCCCTT CCCTTCCTGA TATGTCTCTG 
TCCACACATC CATCTGTTCA GGCAGGTGGG TGGCGAAAGC TCCCGCCTAA TCTCAGCCCC 
ACTATCGAGT ATAATGACGG AAGGCCCACA AAACGGCATG ATATTGCACG CTCCCATTCT 
GAAAGTCCTT CCAGACTACC AATCAACCGG GCGGGAACCT GGAAGCGTGA ACACAGCAAA 
CATTCCTCGT CCCTTCCTCG AGTGAGTACT TGGAGAAGAA CTGGAAGCTC ATCTTCTATT 
CTTTCTGCTT CATCAGAGTC CAGTGAAAAA GCAAAAAGTG AGGATGAAAG GCATGTGAGC 
TCCATGCCAG CACCCAGACA GATGAAGGAA AACCAGGTGC CCACCAAAGG AACATGGAGG 
AAAATCAAGG AAAGTGACAT TTCTCCCACA GGCATGGCTT CTCAGAGCGC TTCCTCAGGT 
GCTGCCAGTG GTGCTGAATC CAAGCCTCTG ATCTATCAGA TGGCACCTCC TGTCTCTAAA 
ACAGAGGATG TTTGGGTGAG AATTGAGGAC TGCCCCATTA ACAACCCTAG ATCTGGACGG 
TCCCCCACAG GCAACACCCC CCCAGTGATT GACAGTGTTT CAGAGAAGGG AAGTTCAAGC 
ATTAAAGATT CAAAAGACAC CCATGGGAAA CAGAGTGTGG GCAGTGGCAG TCCTGTGCAA 
ACCGTGGGTC TGGAAACCCG CCTCAACTCC TTTGTTCAGG TAGAGGCCCC AGAACAGAAA 
GGAACTGAGG CAAAACCAGG ACAGAGTAAC CCAGTCTCTA TAGCAGAGAC TGCTGAGACG 
TGTATAGCAG AGCGTACCCC TTTCAGTTCC AGTAGCTCCA GCAAGCACAG CTCACCTAGC 
GGGACTGTTG CTGCCAGAGT GACACCTTTT AATTACAACC CTAGCCCTAG GAAGAGCAGC 
GCAGACAGCA CTTCAGCCCG GCCGTCTCAG ATCCCTACGC CAGTGAGCAC CAACACGAAG 
AAGAGAGATT CGAAGACTGA CAGCACAGAA TCCAGTGGAG CCCAAAGTCC TAAACGCCAT 
TCCGGGTCTT ACCTCGTGAC GTCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525539.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525602.1 Mus musculus adenomatosis polyposis coli (Apc), transcript variant X11, mRNA.

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CloneID	OMu54324
Clone ID Related Accession (Same CDS sequence)	XM_006525539.2 , XM_006525539.3
Accession Version	XM_006525539.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGATA CGAGCTTACT GTGAAACCTG TTGGGAGTGG 
CAGGAAGCCC ACGAACAAGG CATGGACCAG GACAAAAACC CAATGCCAGC TCCTGTTGAG 
CATCAGATCT GTCCTGCTGT GTGTGTTCTA ATGAAGCTTT CATTTGATGA AGAGCATAGG 
CATGCAATGA ATGAACTTGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525602.1
CDS	312..8537
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525539.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525539.2

ORF Insert Sequence:

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGATA CGAGCTTACT GTGAAACCTG TTGGGAGTGG 
CAGGAAGCCC ACGAACAAGG CATGGACCAG GACAAAAACC CAATGCCAGC TCCTGTTGAG 
CATCAGATCT GTCCTGCTGT GTGTGTTCTA ATGAAGCTTT CATTTGATGA AGAGCATAGG 
CATGCAATGA ATGAACTTGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_006525539.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525602.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X24, mRNA.

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CloneID	OMu54324
Clone ID Related Accession (Same CDS sequence)	XM_006525539.2 , XM_006525539.3
Accession Version	XM_006525539.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525539.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGATA CGAGCTTACT GTGAAACCTG TTGGGAGTGG 
CAGGAAGCCC ACGAACAAGG CATGGACCAG GACAAAAACC CAATGCCAGC TCCTGTTGAG 
CATCAGATCT GTCCTGCTGT GTGTGTTCTA ATGAAGCTTT CATTTGATGA AGAGCATAGG 
CATGCAATGA ATGAACTTGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525602.1
CDS	413..8638
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_006525539.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525539.3

ORF Insert Sequence:

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGATA CGAGCTTACT GTGAAACCTG TTGGGAGTGG 
CAGGAAGCCC ACGAACAAGG CATGGACCAG GACAAAAACC CAATGCCAGC TCCTGTTGAG 
CATCAGATCT GTCCTGCTGT GTGTGTTCTA ATGAAGCTTT CATTTGATGA AGAGCATAGG 
CATGCAATGA ATGAACTTGG GGGACTGCAG GCCATTGCAG AGTTATTGCA GGTGGACTGT 
GAGATGTATG GGCTTACTAA TGACCACTAC AGTGTTACTT TAAGACGGTA TGCTGGAATG 
GCTTTGACAA ACTTGACCTT TGGAGATGTT GCCAACAAGG CTACGCTGTG TTCTATGAAA 
GGCTGCATGA GAGCACTTGT GGCCCAGTTA AAATCTGAGA GTGAAGACTT ACAGCAGGTT 
ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG CGAGCAGATG TAAATAGCAA AAAGACGTTG 
AGAGAAGTTG GAAGTGTGAA AGCATTGATG GAATGTGCTT TGGAAGTTAA AAAGGAATCA 
ACCCTCAAAA GCGTTTTGAG TGCCTTATGG AACCTGTCTG CACACTGCAC TGAGAATAAG 
GCTGACATCT GTGCTGTGGA TGGAGCACTG GCATTTCTGG TTGGCACCCT CACTTACCGG 
AGCCAGACAA ATACTTTAGC CATTATTGAA AGTGGAGGTG GGATATTACG GAATGTGTCC 
AGCTTGATAG CTACAAACGA AGACCACAGG CAAATCCTAA GAGAGAACAA TTGCCTACAA 
ACTTTATTAC AGCACTTGAA ATCTCACAGC TTGACAATAG TCAGTAATGC ATGTGGAACT 
TTGTGGAATC TCTCAGCAAG AAATCCTAAA GACCAGGAAG CCTTGTGGGA CATGGGGGCA 
GTGAGCATGC TCAAGAACCT CATTCATTCC AAGCACAAAA TGATTGCCAT GGGAAGTGCA 
GCAGCTTTAA GGAATCTCAT GGCAAACAGA CCTGCAAAGT ATAAGGATGC CAATATCATG 
TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG 
CTAGATGCTC AGCATTTATC AGAAACCTTC GACAACATTG ACAACCTAAG TCCCAAGGCC 
TCTCACCGGA GTAAGCAGAG ACACAAGCAG AATCTTTATG GTGACTATGC TTTTGACGCC 
AATCGACATG ATGATAGTAG GTCAGACAAT TTCAATACTG GAAACATGAC TGTTCTTTCA 
CCATATTTAA ATACTACGGT ATTGCCCAGC TCTTCTTCCT CAAGGGGAAG TTTAGACAGT 
TCTCGTTCTG AGAAAGACAG AAGTTTGGAG AGAGAGCGAG GTATTGGCCT CAGTGCTTAC 
CATCCAACAA CAGAAAATGC AGGAACCTCA TCAAAACGAG GTCTGCAGAT CACTACCACT 
GCAGCCCAGA TAGCCAAAGT TATGGAAGAA GTATCAGCCA TTCATACCTC CCAGGACGAC 
AGAAGTTCTG CTTCTACCAC CGAGTTCCAT TGTGTGGCAG ACGACAGGAG TGCGGCACGA 
AGAAGCTCTG CCTCCCACAC ACACTCAAAC ACATACAACT TCACTAAGTC GGAAAATTCA 
AATAGGACAT GCTCTATGCC TTATGCCAAA GTGGAATATA AACGATCTTC AAATGACAGT 
TTAAATAGTG TCACTAGTAG TGATGGATAT GGTAAAAGAG GCCAAATGAA ACCCTCAGTT 
GAATCCTATT CTGAAGATGA TGAAAGTAAA TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC 
CTAGCCCATA AGATACACAG TGCAAATCAT ATGGATGATA ATGATGGAGA ACTGGATACA 
CCAATAAATT ACAGTCTTAA ATATTCAGAT GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC 
TCACAGAATG AAAGGTGGGC AAGACCAAAG CATGTGATAG AAGATGAAAT AAAGCAAAAC 
GAGCAAAGAC AAGCAAGAAG CCAGAACACC AGTTATCCTG TCTATTCTGA GAATACCGAT 
GACAAACACC TCAAATTCCA ACCACATTTT GGACAACAAG AATGTGTTTC CCCATATAGG 
TCAAGGGGAA CCAGTGGTTC AGAAACAAAT CGAATGGGTT CTAGTCATGC AATTAATCAA 
AATGTAAACC AGTCTCTGTG TCAGGAAGAT GATTATGAAG ATGATAAACC TACCAACTAC 
AGTGAACGTT ATTCTGAGGA AGAACAACAT GAAGAAGAAG AAGAGAGACC GACAAATTAT 
AGCATAAAAT ATAATGAAGA GAAACATCAT GTGGATCAGC CTATTGATTA TAGTTTAAAA 
TATGCCACTG ACATTTCTTC CTCACAAAAA CCATCATTTT CATTCTCAAA GAATTCATCA 
GCACAAAGCA CTAAACCTGA ACATCTCTCT CCAAGCAGCG AGAATACAGC TGTACCTCCA 
TCTAATGCCA AAAGGCAGAA TCAGCTGCGT CCAAGTTCAG CACAAAGAAA TGGCCAGACT 
CAAAAAGGCA CTACTTGCAA AGTCCCCTCC ATCAACCAAG AAACAATACA GACTTACTGC 
GTAGAAGACA CCCCAATATG TTTTTCAAGG TGCAGTTCAT TATCATCACT GTCATCAGCT 
GACGATGAAA TAGGATGTGA TCAGACAACA CAGGAAGCAG ATTCTGCTAA TACTCTGCAG 
ACAGCAGAAG TAAAAGAGAA TGATGTAACT CGGTCAGCTG AAGATCCTGC AACTGAAGTT 
CCAGCAGTGT CCCAGAATGC TAGAGCCAAA CCCAGCCGAC TCCAGGCTTC TGGCTTATCT 
TCAGAATCAA CCAGGCATAA TAAAGCTGTT GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC 
AAAAGTGGTG CTCAGACACC CAAAAGTCCC CCAGAACACT ATGTCCAGGA GACTCCGCTC 
GTATTCAGCA GGTGTACTTC TGTCAGCTCC CTTGACAGTT TTGAGAGTCG CTCCATTGCC 
AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA ATGGTGAGTG GCATCATAAG CCCCAGTGAC 
CTTCCAGATA GTCCTGGGCA GACCATGCCA CCAAGCAGAA GCAAAACCCC TCCACCTCCT 
CCACAGACAG TGCAGGCCAA GAGAGAGGTG CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG 
AGAGAGAGTG GGCCTAAGCA GACTGCTGTA AATGCTGCCG TGCAGAGGGT GCAGGTCCTT 
CCAGACGTGG ATACTTTGTT ACACTTCGCC ACAGAAAGTA CTCCAGACGG GTTTTCTTGT 
TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT GAGCCATTTA TACAGAAAGA TGTAGAATTA 
AGAATCATGC CTCCAGTTCA GGAAAACGAC AATGGGAATG AAACTGAATC AGAACAGCCT 
GAGGAATCAA ATGAAAACCA GGATAAAGAG GTAGAAAAGC CTGACTCTGA AAAAGACTTA 
TTAGATGATT CTGATGACGA TGATATTGAA ATATTAGAAG AATGTATTAT TTCAGCCATG 
CCAACAAAGT CATCACGCAA AGCCAAAAAA CTAGCCCAGA CTGCTTCAAA ATTACCTCCA 
CCTGTGGCAA GGAAACCAAG TCAGCTACCT GTGTATAAAC TTCTGCCAGC ACAGAATAGG 
CTGCAGGCAC AAAAACATGT TAGCTTTACA CCAGGGGATG ATGTGCCCCG GGTGTACTGT 
GTAGAAGGGA CACCTATAAA CTTTTCCACA GCAACGTCTC TAAGTGATCT GACAATAGAG 
TCCCCTCCAA ATGAATTGGC TACTGGAGAT GGGGTCAGAG CGGGTATACA GTCAGGTGAA 
TTTGAAAAAC GAGATACCAT TCCTACAGAA GGCAGAAGTA CAGATGATGC TCAGCGAGGA 
AAAATCTCAT CTATAGTTAC ACCAGACCTG GATGACAACA AAGCAGAGGA AGGAGATATT 
CTTGCAGAAT GTATCAATTC TGCTATGCCC AAAGGAAAAA GCCACAAGCC TTTCCGAGTG 
AAAAAGATAA TGGACCAAGT CCAACAAGCA TCCTCGACTT CATCTGGAGC TAACAAAAAT 
CAAGTAGACA CTAAGAAAAA GAAGCCTACT TCACCAGTAA AGCCCATGCC ACAAAATACT 
GAATATAGAA CGCGTGTGAG AAAGAATACA GACTCAAAAG TTAATGTAAA TACTGAAGAA 
ACTTTCTCAG ACAACAAAGA CTCAAAGAAA CCAAGCTTAC AAACCAATGC CAAGGCCTTC 
AATGAAAAGC TACCTAACAA TGAAGACAGA GTGCGGGGGA GCTTCGCCTT GGACTCACCG 
CATCACTACA CCCCTATTGA GGGGACGCCG TACTGCTTTT CCCGAAATGA CTCCTTGAGT 
TCTCTGGATT TTGATGATGA CGATGTTGAC CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG 
GGCAAAGAAA GCAAGGATTC CGAAGCCAAA GTTACCTGCC GCCCAGAACC AAACTCAAGC 
CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT ATAAAACATC CAGCAAACAG AGCACAGTCC 
AAACCAGTGC TGCAGAAACA GCCCACTTTC CCCCAGTCCT CCAAAGACGG ACCAGATAGA 
GGGGCAGCAA CTGACGAAAA ACTGCAGAAT TTTGCTATTG AAAATACTCC AGTTTGCTTT 
TCTCGAAATT CCTCTCTGAG TTCCCTTAGT GACATTGACC AGGAAAACAA CAATAACAAA 
GAAAGTGAAC CAATCAAAGA AGCTGAACCT GCCAACTCAC AAGGAGAGCC CAGTAAGCCT 
CAGGCATCCG GGTATGCTCC CAAGTCCTTC CACGTCGAAG ACACCCCTGT CTGTTTCTCA 
AGAAACAGCT CTCTCAGTTC TCTTAGCATT GACTCTGAGG ACGACCTGTT ACAGGAGTGT 
ATAAGTTCTG CCATGCCAAA AAAGAAAAGG CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG 
CAGAGCCCTA GAAAAGTGGG TGGCATATTA GCTGAAGACC TGACGCTTGA TTTGAAAGAT 
CTACAGAGGC CAGATTCAGA ACACGCTTTC TCCCCCGACT CAGAAAATTT TGACTGGAAA 
GCTATTCAGG AAGGCGCAAA CTCCATAGTA AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC 
GCGTGCTTAT CTAGACAAGC GTCATCCGAC TCAGATTCCA TTCTGTCACT AAAGTCCGGC 
ATTTCTCTGG GATCGCCTTT TCATCTTACA CCTGATCAAG AGGAAAAGCC ATTCACAAGC 
AATAAAGGCC CAAGAATTCT CAAACCTGGA GAGAAAAGCA CATTAGAAGC AAAAAAAATA 
GAATCTGAAA ACAAAGGAAT CAAAGGCGGG AAAAAGGTTT ATAAAAGCTT GATTACGGGA 
AAGATTCGCT CCAATTCAGA AATTTCCAGC CAAATGAAAC AACCCCTCCC GACAAACATG 
CCTTCAATCT CAAGAGGCAG GACGATGATT CACATCCCAG GGCTTCGGAA TAGCTCCTCT 
AGTACAAGCC CTGTCTCTAA GAAAGGCCCA CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC 
AGTGAAGGGC CGGGAGCTAC CACTTCTCCT CGAGGAACTA AGCCAGCAGG AAAGTCAGAG 
CTTAGCCCTA TCACCAGGCA AACTTCCCAA ATCAGTGGGT CAAATAAGGG GTCTTCTAGA 
TCAGGATCTA GAGACTCCAC TCCCTCAAGA CCTACACAGC AACCATTAAG TAGGCCAATG 
CAGTCTCCAG GGCGAAACTC AATTTCCCCT GGTAGAAATG GAATAAGCCC TCCTAACAAA 
CTGTCTCAGC TGCCCAGAAC ATCATCTCCC AGTACTGCTT CAACTAAGTC CTCCGGTTCT 
GGGAAAATGT CATATACATC CCCAGGTAGA CAGCTGAGCC AACAAAATCT TACCAAACAA 
GCAAGTTTAT CCAAGAATGC CAGCAGTATC CCCAGAAGTG AGTCGGCATC TAAAGGACTG 
AATCAGATGA GTAACGGCAA TGGGTCAAAT AAAAAGGTAG AACTTTCTAG AATGTCTTCA 
ACTAAATCAA GTGGAAGTGA ATCAGACAGA TCAGAAAGGC CTGCATTAGT ACGCCAGTCT 
ACTTTCATCA AAGAAGCCCC AAGCCCAACC CTGAGGAGGA AACTGGAGGA ATCTGCCTCA 
TTTGAATCCC TTTCTCCATC TTCTAGACCA GATTCTCCCA CCAGGTCGCA GGCACAGACC 
CCAGTTTTAA GCCCTTCCCT TCCTGATATG TCTCTGTCCA CACATCCATC TGTTCAGGCA 
GGTGGGTGGC GAAAGCTCCC GCCTAATCTC AGCCCCACTA TCGAGTATAA TGACGGAAGG 
CCCACAAAAC GGCATGATAT TGCACGCTCC CATTCTGAAA GTCCTTCCAG ACTACCAATC 
AACCGGGCGG GAACCTGGAA GCGTGAACAC AGCAAACATT CCTCGTCCCT TCCTCGAGTG 
AGTACTTGGA GAAGAACTGG AAGCTCATCT TCTATTCTTT CTGCTTCATC AGAGTCCAGT 
GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT GTGAGCTCCA TGCCAGCACC CAGACAGATG 
AAGGAAAACC AGGTGCCCAC CAAAGGAACA TGGAGGAAAA TCAAGGAAAG TGACATTTCT 
CCCACAGGCA TGGCTTCTCA GAGCGCTTCC TCAGGTGCTG CCAGTGGTGC TGAATCCAAG 
CCTCTGATCT ATCAGATGGC ACCTCCTGTC TCTAAAACAG AGGATGTTTG GGTGAGAATT 
GAGGACTGCC CCATTAACAA CCCTAGATCT GGACGGTCCC CCACAGGCAA CACCCCCCCA 
GTGATTGACA GTGTTTCAGA GAAGGGAAGT TCAAGCATTA AAGATTCAAA AGACACCCAT 
GGGAAACAGA GTGTGGGCAG TGGCAGTCCT GTGCAAACCG TGGGTCTGGA AACCCGCCTC 
AACTCCTTTG TTCAGGTAGA GGCCCCAGAA CAGAAAGGAA CTGAGGCAAA ACCAGGACAG 
AGTAACCCAG TCTCTATAGC AGAGACTGCT GAGACGTGTA TAGCAGAGCG TACCCCTTTC 
AGTTCCAGTA GCTCCAGCAA GCACAGCTCA CCTAGCGGGA CTGTTGCTGC CAGAGTGACA 
CCTTTTAATT ACAACCCTAG CCCTAGGAAG AGCAGCGCAG ACAGCACTTC AGCCCGGCCG 
TCTCAGATCC CTACGCCAGT GAGCACCAAC ACGAAGAAGA GAGATTCGAA GACTGACAGC 
ACAGAATCCA GTGGAGCCCA AAGTCCTAAA CGCCATTCCG GGTCTTACCT CGTGACGTCT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250287.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106147.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X17, mRNA.

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CloneID	OMu79754
Clone ID Related Accession (Same CDS sequence)	XM_030250287.1 , XM_030250286.1 , NM_001360979.1 , XM_017317804.1
Accession Version	XM_030250287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGGGGA CTGCAGGCCA TTGCAGAGTT ATTGCAGGTG 
GACTGTGAGA TGTATGGGCT TACTAATGAC CACTACAGTG TTACTTTAAG ACGGTATGCT 
GGAATGGCTT TGACAAACTT GACCTTTGGA GATGTTGCCA ACAAGGCTAC GCTGTGTTCT 
ATGAAAGGCT GCATGAGAGC ACTTGTGGCC CAGTTAAAAT CTGAGAGTGA AGACTTACAG 
CAGGTTATTG CAAGTGTTTT GAGGAATTTG TCTTGGCGAG CAGATGTAAA TAGCAAAAAG 
ACGTTGAGAG AAGTTGGAAG TGTGAAAGCA TTGATGGAAT GTGCTTTGGA AGTTAAAAAG 
GAATCAACCC TCAAAAGCGT TTTGAGTGCC TTATGGAACC TGTCTGCACA CTGCACTGAG 
AATAAGGCTG ACATCTGTGC TGTGGATGGA GCACTGGCAT TTCTGGTTGG CACCCTCACT 
TACCGGAGCC AGACAAATAC TTTAGCCATT ATTGAAAGTG GAGGTGGGAT ATTACGGAAT 
GTGTCCAGCT TGATAGCTAC AAACGAAGAC CACAGGCAAA TCCTAAGAGA GAACAATTGC 
CTACAAACTT TATTACAGCA CTTGAAATCT CACAGCTTGA CAATAGTCAG TAATGCATGT 
GGAACTTTGT GGAATCTCTC AGCAAGAAAT CCTAAAGACC AGGAAGCCTT GTGGGACATG 
GGGGCAGTGA GCATGCTCAA GAACCTCATT CATTCCAAGC ACAAAATGAT TGCCATGGGA 
AGTGCAGCAG CTTTAAGGAA TCTCATGGCA AACAGACCTG CAAAGTATAA GGATGCCAAT 
ATCATGTCTC CCGGCTCAAG TCTGCCATCC CTTCACGTTA GGAAACAGAA AGCTCTAGAA 
GCTGAGCTAG ATGCTCAGCA TTTATCAGAA ACCTTCGACA ACATTGACAA CCTAAGTCCC 
AAGGCCTCTC ACCGGAGTAA GCAGAGACAC AAGCAGAATC TTTATGGTGA CTATGCTTTT 
GACGCCAATC GACATGATGA TAGTAGGTCA GACAATTTCA ATACTGGAAA CATGACTGTT 
CTTTCACCAT ATTTAAATAC TACGGTATTG CCCAGCTCTT CTTCCTCAAG GGGAAGTTTA 
GACAGTTCTC GTTCTGAGAA AGACAGAAGT TTGGAGAGAG AGCGAGGTAT TGGCCTCAGT 
GCTTACCATC CAACAACAGA AAATGCAGGA ACCTCATCAA AACGAGGTCT GCAGATCACT 
ACCACTGCAG CCCAGATAGC CAAAGTTATG GAAGAAGTAT CAGCCATTCA TACCTCCCAG 
GACGACAGAA GTTCTGCTTC TACCACCGAG TTCCATTGTG TGGCAGACGA CAGGAGTGCG 
GCACGAAGAA GCTCTGCCTC CCACACACAC TCAAACACAT ACAACTTCAC TAAGTCGGAA 
AATTCAAATA GGACATGCTC TATGCCTTAT GCCAAAGTGG AATATAAACG ATCTTCAAAT 
GACAGTTTAA ATAGTGTCAC TAGTAGTGAT GGATATGGTA AAAGAGGCCA AATGAAACCC 
TCAGTTGAAT CCTATTCTGA AGATGATGAA AGTAAATTTT GCAGTTATGG TCAGTATCCA 
GCTGACCTAG CCCATAAGAT ACACAGTGCA AATCATATGG ATGATAATGA TGGAGAACTG 
GATACACCAA TAAATTACAG TCTTAAATAT TCAGATGAGC AGTTGAACTC AGGAAGGCAG 
AGTCCCTCAC AGAATGAAAG GTGGGCAAGA CCAAAGCATG TGATAGAAGA TGAAATAAAG 
CAAAACGAGC AAAGACAAGC AAGAAGCCAG AACACCAGTT ATCCTGTCTA TTCTGAGAAT 
ACCGATGACA AACACCTCAA ATTCCAACCA CATTTTGGAC AACAAGAATG TGTTTCCCCA 
TATAGGTCAA GGGGAACCAG TGGTTCAGAA ACAAATCGAA TGGGTTCTAG TCATGCAATT 
AATCAAAATG TAAACCAGTC TCTGTGTCAG GAAGATGATT ATGAAGATGA TAAACCTACC 
AACTACAGTG AACGTTATTC TGAGGAAGAA CAACATGAAG AAGAAGAAGA GAGACCGACA 
AATTATAGCA TAAAATATAA TGAAGAGAAA CATCATGTGG ATCAGCCTAT TGATTATAGT 
TTAAAATATG CCACTGACAT TTCTTCCTCA CAAAAACCAT CATTTTCATT CTCAAAGAAT 
TCATCAGCAC AAAGCACTAA ACCTGAACAT CTCTCTCCAA GCAGCGAGAA TACAGCTGTA 
CCTCCATCTA ATGCCAAAAG GCAGAATCAG CTGCGTCCAA GTTCAGCACA AAGAAATGGC 
CAGACTCAAA AAGGCACTAC TTGCAAAGTC CCCTCCATCA ACCAAGAAAC AATACAGACT 
TACTGCGTAG AAGACACCCC AATATGTTTT TCAAGGTGCA GTTCATTATC ATCACTGTCA 
TCAGCTGACG ATGAAATAGG ATGTGATCAG ACAACACAGG AAGCAGATTC TGCTAATACT 
CTGCAGACAG CAGAAGTAAA AGAGAATGAT GTAACTCGGT CAGCTGAAGA TCCTGCAACT 
GAAGTTCCAG CAGTGTCCCA GAATGCTAGA GCCAAACCCA GCCGACTCCA GGCTTCTGGC 
TTATCTTCAG AATCAACCAG GCATAATAAA GCTGTTGAGT TTTCTTCAGG AGCCAAGTCT 
CCCTCCAAAA GTGGTGCTCA GACACCCAAA AGTCCCCCAG AACACTATGT CCAGGAGACT 
CCGCTCGTAT TCAGCAGGTG TACTTCTGTC AGCTCCCTTG ACAGTTTTGA GAGTCGCTCC 
ATTGCCAGCT CTGTTCAGAG TGAGCCATGT AGTGGAATGG TGAGTGGCAT CATAAGCCCC 
AGTGACCTTC CAGATAGTCC TGGGCAGACC ATGCCACCAA GCAGAAGCAA AACCCCTCCA 
CCTCCTCCAC AGACAGTGCA GGCCAAGAGA GAGGTGCCAA AAAGTAAAGT CCCTGCTGCT 
GAGAAGAGAG AGAGTGGGCC TAAGCAGACT GCTGTAAATG CTGCCGTGCA GAGGGTGCAG 
GTCCTTCCAG ACGTGGATAC TTTGTTACAC TTCGCCACAG AAAGTACTCC AGACGGGTTT 
TCTTGTTCCT CCAGCCTAAG TGCTCTGAGC CTGGATGAGC CATTTATACA GAAAGATGTA 
GAATTAAGAA TCATGCCTCC AGTTCAGGAA AACGACAATG GGAATGAAAC TGAATCAGAA 
CAGCCTGAGG AATCAAATGA AAACCAGGAT AAAGAGGTAG AAAAGCCTGA CTCTGAAAAA 
GACTTATTAG ATGATTCTGA TGACGATGAT ATTGAAATAT TAGAAGAATG TATTATTTCA 
GCCATGCCAA CAAAGTCATC ACGCAAAGCC AAAAAACTAG CCCAGACTGC TTCAAAATTA 
CCTCCACCTG TGGCAAGGAA ACCAAGTCAG CTACCTGTGT ATAAACTTCT GCCAGCACAG 
AATAGGCTGC AGGCACAAAA ACATGTTAGC TTTACACCAG GGGATGATGT GCCCCGGGTG 
TACTGTGTAG AAGGGACACC TATAAACTTT TCCACAGCAA CGTCTCTAAG TGATCTGACA 
ATAGAGTCCC CTCCAAATGA ATTGGCTACT GGAGATGGGG TCAGAGCGGG TATACAGTCA 
GGTGAATTTG AAAAACGAGA TACCATTCCT ACAGAAGGCA GAAGTACAGA TGATGCTCAG 
CGAGGAAAAA TCTCATCTAT AGTTACACCA GACCTGGATG ACAACAAAGC AGAGGAAGGA 
GATATTCTTG CAGAATGTAT CAATTCTGCT ATGCCCAAAG GAAAAAGCCA CAAGCCTTTC 
CGAGTGAAAA AGATAATGGA CCAAGTCCAA CAAGCATCCT CGACTTCATC TGGAGCTAAC 
AAAAATCAAG TAGACACTAA GAAAAAGAAG CCTACTTCAC CAGTAAAGCC CATGCCACAA 
AATACTGAAT ATAGAACGCG TGTGAGAAAG AATACAGACT CAAAAGTTAA TGTAAATACT 
GAAGAAACTT TCTCAGACAA CAAAGACTCA AAGAAACCAA GCTTACAAAC CAATGCCAAG 
GCCTTCAATG AAAAGCTACC TAACAATGAA GACAGAGTGC GGGGGAGCTT CGCCTTGGAC 
TCACCGCATC ACTACACCCC TATTGAGGGG ACGCCGTACT GCTTTTCCCG AAATGACTCC 
TTGAGTTCTC TGGATTTTGA TGATGACGAT GTTGACCTTT CCAGGGAAAA GGCCGAGTTA 
AGAAAGGGCA AAGAAAGCAA GGATTCCGAA GCCAAAGTTA CCTGCCGCCC AGAACCAAAC 
TCAAGCCAGC AGGCAGCTAG TAAGTCACAA GCCAGTATAA AACATCCAGC AAACAGAGCA 
CAGTCCAAAC CAGTGCTGCA GAAACAGCCC ACTTTCCCCC AGTCCTCCAA AGACGGACCA 
GATAGAGGGG CAGCAACTGA CGAAAAACTG CAGAATTTTG CTATTGAAAA TACTCCAGTT 
TGCTTTTCTC GAAATTCCTC TCTGAGTTCC CTTAGTGACA TTGACCAGGA AAACAACAAT 
AACAAAGAAA GTGAACCAAT CAAAGAAGCT GAACCTGCCA ACTCACAAGG AGAGCCCAGT 
AAGCCTCAGG CATCCGGGTA TGCTCCCAAG TCCTTCCACG TCGAAGACAC CCCTGTCTGT 
TTCTCAAGAA ACAGCTCTCT CAGTTCTCTT AGCATTGACT CTGAGGACGA CCTGTTACAG 
GAGTGTATAA GTTCTGCCAT GCCAAAAAAG AAAAGGCCTT CAAGACTCAA GAGTGAGAGC 
GAAAAGCAGA GCCCTAGAAA AGTGGGTGGC ATATTAGCTG AAGACCTGAC GCTTGATTTG 
AAAGATCTAC AGAGGCCAGA TTCAGAACAC GCTTTCTCCC CCGACTCAGA AAATTTTGAC 
TGGAAAGCTA TTCAGGAAGG CGCAAACTCC ATAGTAAGTA GTTTGCACCA AGCTGCTGCA 
GCCGCCGCGT GCTTATCTAG ACAAGCGTCA TCCGACTCAG ATTCCATTCT GTCACTAAAG 
TCCGGCATTT CTCTGGGATC GCCTTTTCAT CTTACACCTG ATCAAGAGGA AAAGCCATTC 
ACAAGCAATA AAGGCCCAAG AATTCTCAAA CCTGGAGAGA AAAGCACATT AGAAGCAAAA 
AAAATAGAAT CTGAAAACAA AGGAATCAAA GGCGGGAAAA AGGTTTATAA AAGCTTGATT 
ACGGGAAAGA TTCGCTCCAA TTCAGAAATT TCCAGCCAAA TGAAACAACC CCTCCCGACA 
AACATGCCTT CAATCTCAAG AGGCAGGACG ATGATTCACA TCCCAGGGCT TCGGAATAGC 
TCCTCTAGTA CAAGCCCTGT CTCTAAGAAA GGCCCACCCC TCAAGACTCC AGCCTCTAAA 
AGCCCCAGTG AAGGGCCGGG AGCTACCACT TCTCCTCGAG GAACTAAGCC AGCAGGAAAG 
TCAGAGCTTA GCCCTATCAC CAGGCAAACT TCCCAAATCA GTGGGTCAAA TAAGGGGTCT 
TCTAGATCAG GATCTAGAGA CTCCACTCCC TCAAGACCTA CACAGCAACC ATTAAGTAGG 
CCAATGCAGT CTCCAGGGCG AAACTCAATT TCCCCTGGTA GAAATGGAAT AAGCCCTCCT 
AACAAACTGT CTCAGCTGCC CAGAACATCA TCTCCCAGTA CTGCTTCAAC TAAGTCCTCC 
GGTTCTGGGA AAATGTCATA TACATCCCCA GGTAGACAGC TGAGCCAACA AAATCTTACC 
AAACAAGCAA GTTTATCCAA GAATGCCAGC AGTATCCCCA GAAGTGAGTC GGCATCTAAA 
GGACTGAATC AGATGAGTAA CGGCAATGGG TCAAATAAAA AGGTAGAACT TTCTAGAATG 
TCTTCAACTA AATCAAGTGG AAGTGAATCA GACAGATCAG AAAGGCCTGC ATTAGTACGC 
CAGTCTACTT TCATCAAAGA AGCCCCAAGC CCAACCCTGA GGAGGAAACT GGAGGAATCT 
GCCTCATTTG AATCCCTTTC TCCATCTTCT AGACCAGATT CTCCCACCAG GTCGCAGGCA 
CAGACCCCAG TTTTAAGCCC TTCCCTTCCT GATATGTCTC TGTCCACACA TCCATCTGTT 
CAGGCAGGTG GGTGGCGAAA GCTCCCGCCT AATCTCAGCC CCACTATCGA GTATAATGAC 
GGAAGGCCCA CAAAACGGCA TGATATTGCA CGCTCCCATT CTGAAAGTCC TTCCAGACTA 
CCAATCAACC GGGCGGGAAC CTGGAAGCGT GAACACAGCA AACATTCCTC GTCCCTTCCT 
CGAGTGAGTA CTTGGAGAAG AACTGGAAGC TCATCTTCTA TTCTTTCTGC TTCATCAGAG 
TCCAGTGAAA AAGCAAAAAG TGAGGATGAA AGGCATGTGA GCTCCATGCC AGCACCCAGA 
CAGATGAAGG AAAACCAGGT GCCCACCAAA GGAACATGGA GGAAAATCAA GGAAAGTGAC 
ATTTCTCCCA CAGGCATGGC TTCTCAGAGC GCTTCCTCAG GTGCTGCCAG TGGTGCTGAA 
TCCAAGCCTC TGATCTATCA GATGGCACCT CCTGTCTCTA AAACAGAGGA TGTTTGGGTG 
AGAATTGAGG ACTGCCCCAT TAACAACCCT AGATCTGGAC GGTCCCCCAC AGGCAACACC 
CCCCCAGTGA TTGACAGTGT TTCAGAGAAG GGAAGTTCAA GCATTAAAGA TTCAAAAGAC 
ACCCATGGGA AACAGAGTGT GGGCAGTGGC AGTCCTGTGC AAACCGTGGG TCTGGAAACC 
CGCCTCAACT CCTTTGTTCA GGTAGAGGCC CCAGAACAGA AAGGAACTGA GGCAAAACCA 
GGACAGAGTA ACCCAGTCTC TATAGCAGAG ACTGCTGAGA CGTGTATAGC AGAGCGTACC 
CCTTTCAGTT CCAGTAGCTC CAGCAAGCAC AGCTCACCTA GCGGGACTGT TGCTGCCAGA 
GTGACACCTT TTAATTACAA CCCTAGCCCT AGGAAGAGCA GCGCAGACAG CACTTCAGCC 
CGGCCGTCTC AGATCCCTAC GCCAGTGAGC ACCAACACGA AGAAGAGAGA TTCGAAGACT 
GACAGCACAG AATCCAGTGG AGCCCAAAGT CCTAAACGCC ATTCCGGGTC TTACCTCGTG 
ACGTCTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106147.1
CDS	460..8811
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250287.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250287.1

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGGGGA CTGCAGGCCA TTGCAGAGTT ATTGCAGGTG 
GACTGTGAGA TGTATGGGCT TACTAATGAC CACTACAGTG TTACTTTAAG ACGGTATGCT 
GGAATGGCTT TGACAAACTT GACCTTTGGA GATGTTGCCA ACAAGGCTAC GCTGTGTTCT 
ATGAAAGGCT GCATGAGAGC ACTTGTGGCC CAGTTAAAAT CTGAGAGTGA AGACTTACAG 
CAGGTTATTG CAAGTGTTTT GAGGAATTTG TCTTGGCGAG CAGATGTAAA TAGCAAAAAG 
ACGTTGAGAG AAGTTGGAAG TGTGAAAGCA TTGATGGAAT GTGCTTTGGA AGTTAAAAAG 
GAATCAACCC TCAAAAGCGT TTTGAGTGCC TTATGGAACC TGTCTGCACA CTGCACTGAG 
AATAAGGCTG ACATCTGTGC TGTGGATGGA GCACTGGCAT TTCTGGTTGG CACCCTCACT 
TACCGGAGCC AGACAAATAC TTTAGCCATT ATTGAAAGTG GAGGTGGGAT ATTACGGAAT 
GTGTCCAGCT TGATAGCTAC AAACGAAGAC CACAGGCAAA TCCTAAGAGA GAACAATTGC 
CTACAAACTT TATTACAGCA CTTGAAATCT CACAGCTTGA CAATAGTCAG TAATGCATGT 
GGAACTTTGT GGAATCTCTC AGCAAGAAAT CCTAAAGACC AGGAAGCCTT GTGGGACATG 
GGGGCAGTGA GCATGCTCAA GAACCTCATT CATTCCAAGC ACAAAATGAT TGCCATGGGA 
AGTGCAGCAG CTTTAAGGAA TCTCATGGCA AACAGACCTG CAAAGTATAA GGATGCCAAT 
ATCATGTCTC CCGGCTCAAG TCTGCCATCC CTTCACGTTA GGAAACAGAA AGCTCTAGAA 
GCTGAGCTAG ATGCTCAGCA TTTATCAGAA ACCTTCGACA ACATTGACAA CCTAAGTCCC 
AAGGCCTCTC ACCGGAGTAA GCAGAGACAC AAGCAGAATC TTTATGGTGA CTATGCTTTT 
GACGCCAATC GACATGATGA TAGTAGGTCA GACAATTTCA ATACTGGAAA CATGACTGTT 
CTTTCACCAT ATTTAAATAC TACGGTATTG CCCAGCTCTT CTTCCTCAAG GGGAAGTTTA 
GACAGTTCTC GTTCTGAGAA AGACAGAAGT TTGGAGAGAG AGCGAGGTAT TGGCCTCAGT 
GCTTACCATC CAACAACAGA AAATGCAGGA ACCTCATCAA AACGAGGTCT GCAGATCACT 
ACCACTGCAG CCCAGATAGC CAAAGTTATG GAAGAAGTAT CAGCCATTCA TACCTCCCAG 
GACGACAGAA GTTCTGCTTC TACCACCGAG TTCCATTGTG TGGCAGACGA CAGGAGTGCG 
GCACGAAGAA GCTCTGCCTC CCACACACAC TCAAACACAT ACAACTTCAC TAAGTCGGAA 
AATTCAAATA GGACATGCTC TATGCCTTAT GCCAAAGTGG AATATAAACG ATCTTCAAAT 
GACAGTTTAA ATAGTGTCAC TAGTAGTGAT GGATATGGTA AAAGAGGCCA AATGAAACCC 
TCAGTTGAAT CCTATTCTGA AGATGATGAA AGTAAATTTT GCAGTTATGG TCAGTATCCA 
GCTGACCTAG CCCATAAGAT ACACAGTGCA AATCATATGG ATGATAATGA TGGAGAACTG 
GATACACCAA TAAATTACAG TCTTAAATAT TCAGATGAGC AGTTGAACTC AGGAAGGCAG 
AGTCCCTCAC AGAATGAAAG GTGGGCAAGA CCAAAGCATG TGATAGAAGA TGAAATAAAG 
CAAAACGAGC AAAGACAAGC AAGAAGCCAG AACACCAGTT ATCCTGTCTA TTCTGAGAAT 
ACCGATGACA AACACCTCAA ATTCCAACCA CATTTTGGAC AACAAGAATG TGTTTCCCCA 
TATAGGTCAA GGGGAACCAG TGGTTCAGAA ACAAATCGAA TGGGTTCTAG TCATGCAATT 
AATCAAAATG TAAACCAGTC TCTGTGTCAG GAAGATGATT ATGAAGATGA TAAACCTACC 
AACTACAGTG AACGTTATTC TGAGGAAGAA CAACATGAAG AAGAAGAAGA GAGACCGACA 
AATTATAGCA TAAAATATAA TGAAGAGAAA CATCATGTGG ATCAGCCTAT TGATTATAGT 
TTAAAATATG CCACTGACAT TTCTTCCTCA CAAAAACCAT CATTTTCATT CTCAAAGAAT 
TCATCAGCAC AAAGCACTAA ACCTGAACAT CTCTCTCCAA GCAGCGAGAA TACAGCTGTA 
CCTCCATCTA ATGCCAAAAG GCAGAATCAG CTGCGTCCAA GTTCAGCACA AAGAAATGGC 
CAGACTCAAA AAGGCACTAC TTGCAAAGTC CCCTCCATCA ACCAAGAAAC AATACAGACT 
TACTGCGTAG AAGACACCCC AATATGTTTT TCAAGGTGCA GTTCATTATC ATCACTGTCA 
TCAGCTGACG ATGAAATAGG ATGTGATCAG ACAACACAGG AAGCAGATTC TGCTAATACT 
CTGCAGACAG CAGAAGTAAA AGAGAATGAT GTAACTCGGT CAGCTGAAGA TCCTGCAACT 
GAAGTTCCAG CAGTGTCCCA GAATGCTAGA GCCAAACCCA GCCGACTCCA GGCTTCTGGC 
TTATCTTCAG AATCAACCAG GCATAATAAA GCTGTTGAGT TTTCTTCAGG AGCCAAGTCT 
CCCTCCAAAA GTGGTGCTCA GACACCCAAA AGTCCCCCAG AACACTATGT CCAGGAGACT 
CCGCTCGTAT TCAGCAGGTG TACTTCTGTC AGCTCCCTTG ACAGTTTTGA GAGTCGCTCC 
ATTGCCAGCT CTGTTCAGAG TGAGCCATGT AGTGGAATGG TGAGTGGCAT CATAAGCCCC 
AGTGACCTTC CAGATAGTCC TGGGCAGACC ATGCCACCAA GCAGAAGCAA AACCCCTCCA 
CCTCCTCCAC AGACAGTGCA GGCCAAGAGA GAGGTGCCAA AAAGTAAAGT CCCTGCTGCT 
GAGAAGAGAG AGAGTGGGCC TAAGCAGACT GCTGTAAATG CTGCCGTGCA GAGGGTGCAG 
GTCCTTCCAG ACGTGGATAC TTTGTTACAC TTCGCCACAG AAAGTACTCC AGACGGGTTT 
TCTTGTTCCT CCAGCCTAAG TGCTCTGAGC CTGGATGAGC CATTTATACA GAAAGATGTA 
GAATTAAGAA TCATGCCTCC AGTTCAGGAA AACGACAATG GGAATGAAAC TGAATCAGAA 
CAGCCTGAGG AATCAAATGA AAACCAGGAT AAAGAGGTAG AAAAGCCTGA CTCTGAAAAA 
GACTTATTAG ATGATTCTGA TGACGATGAT ATTGAAATAT TAGAAGAATG TATTATTTCA 
GCCATGCCAA CAAAGTCATC ACGCAAAGCC AAAAAACTAG CCCAGACTGC TTCAAAATTA 
CCTCCACCTG TGGCAAGGAA ACCAAGTCAG CTACCTGTGT ATAAACTTCT GCCAGCACAG 
AATAGGCTGC AGGCACAAAA ACATGTTAGC TTTACACCAG GGGATGATGT GCCCCGGGTG 
TACTGTGTAG AAGGGACACC TATAAACTTT TCCACAGCAA CGTCTCTAAG TGATCTGACA 
ATAGAGTCCC CTCCAAATGA ATTGGCTACT GGAGATGGGG TCAGAGCGGG TATACAGTCA 
GGTGAATTTG AAAAACGAGA TACCATTCCT ACAGAAGGCA GAAGTACAGA TGATGCTCAG 
CGAGGAAAAA TCTCATCTAT AGTTACACCA GACCTGGATG ACAACAAAGC AGAGGAAGGA 
GATATTCTTG CAGAATGTAT CAATTCTGCT ATGCCCAAAG GAAAAAGCCA CAAGCCTTTC 
CGAGTGAAAA AGATAATGGA CCAAGTCCAA CAAGCATCCT CGACTTCATC TGGAGCTAAC 
AAAAATCAAG TAGACACTAA GAAAAAGAAG CCTACTTCAC CAGTAAAGCC CATGCCACAA 
AATACTGAAT ATAGAACGCG TGTGAGAAAG AATACAGACT CAAAAGTTAA TGTAAATACT 
GAAGAAACTT TCTCAGACAA CAAAGACTCA AAGAAACCAA GCTTACAAAC CAATGCCAAG 
GCCTTCAATG AAAAGCTACC TAACAATGAA GACAGAGTGC GGGGGAGCTT CGCCTTGGAC 
TCACCGCATC ACTACACCCC TATTGAGGGG ACGCCGTACT GCTTTTCCCG AAATGACTCC 
TTGAGTTCTC TGGATTTTGA TGATGACGAT GTTGACCTTT CCAGGGAAAA GGCCGAGTTA 
AGAAAGGGCA AAGAAAGCAA GGATTCCGAA GCCAAAGTTA CCTGCCGCCC AGAACCAAAC 
TCAAGCCAGC AGGCAGCTAG TAAGTCACAA GCCAGTATAA AACATCCAGC AAACAGAGCA 
CAGTCCAAAC CAGTGCTGCA GAAACAGCCC ACTTTCCCCC AGTCCTCCAA AGACGGACCA 
GATAGAGGGG CAGCAACTGA CGAAAAACTG CAGAATTTTG CTATTGAAAA TACTCCAGTT 
TGCTTTTCTC GAAATTCCTC TCTGAGTTCC CTTAGTGACA TTGACCAGGA AAACAACAAT 
AACAAAGAAA GTGAACCAAT CAAAGAAGCT GAACCTGCCA ACTCACAAGG AGAGCCCAGT 
AAGCCTCAGG CATCCGGGTA TGCTCCCAAG TCCTTCCACG TCGAAGACAC CCCTGTCTGT 
TTCTCAAGAA ACAGCTCTCT CAGTTCTCTT AGCATTGACT CTGAGGACGA CCTGTTACAG 
GAGTGTATAA GTTCTGCCAT GCCAAAAAAG AAAAGGCCTT CAAGACTCAA GAGTGAGAGC 
GAAAAGCAGA GCCCTAGAAA AGTGGGTGGC ATATTAGCTG AAGACCTGAC GCTTGATTTG 
AAAGATCTAC AGAGGCCAGA TTCAGAACAC GCTTTCTCCC CCGACTCAGA AAATTTTGAC 
TGGAAAGCTA TTCAGGAAGG CGCAAACTCC ATAGTAAGTA GTTTGCACCA AGCTGCTGCA 
GCCGCCGCGT GCTTATCTAG ACAAGCGTCA TCCGACTCAG ATTCCATTCT GTCACTAAAG 
TCCGGCATTT CTCTGGGATC GCCTTTTCAT CTTACACCTG ATCAAGAGGA AAAGCCATTC 
ACAAGCAATA AAGGCCCAAG AATTCTCAAA CCTGGAGAGA AAAGCACATT AGAAGCAAAA 
AAAATAGAAT CTGAAAACAA AGGAATCAAA GGCGGGAAAA AGGTTTATAA AAGCTTGATT 
ACGGGAAAGA TTCGCTCCAA TTCAGAAATT TCCAGCCAAA TGAAACAACC CCTCCCGACA 
AACATGCCTT CAATCTCAAG AGGCAGGACG ATGATTCACA TCCCAGGGCT TCGGAATAGC 
TCCTCTAGTA CAAGCCCTGT CTCTAAGAAA GGCCCACCCC TCAAGACTCC AGCCTCTAAA 
AGCCCCAGTG AAGGGCCGGG AGCTACCACT TCTCCTCGAG GAACTAAGCC AGCAGGAAAG 
TCAGAGCTTA GCCCTATCAC CAGGCAAACT TCCCAAATCA GTGGGTCAAA TAAGGGGTCT 
TCTAGATCAG GATCTAGAGA CTCCACTCCC TCAAGACCTA CACAGCAACC ATTAAGTAGG 
CCAATGCAGT CTCCAGGGCG AAACTCAATT TCCCCTGGTA GAAATGGAAT AAGCCCTCCT 
AACAAACTGT CTCAGCTGCC CAGAACATCA TCTCCCAGTA CTGCTTCAAC TAAGTCCTCC 
GGTTCTGGGA AAATGTCATA TACATCCCCA GGTAGACAGC TGAGCCAACA AAATCTTACC 
AAACAAGCAA GTTTATCCAA GAATGCCAGC AGTATCCCCA GAAGTGAGTC GGCATCTAAA 
GGACTGAATC AGATGAGTAA CGGCAATGGG TCAAATAAAA AGGTAGAACT TTCTAGAATG 
TCTTCAACTA AATCAAGTGG AAGTGAATCA GACAGATCAG AAAGGCCTGC ATTAGTACGC 
CAGTCTACTT TCATCAAAGA AGCCCCAAGC CCAACCCTGA GGAGGAAACT GGAGGAATCT 
GCCTCATTTG AATCCCTTTC TCCATCTTCT AGACCAGATT CTCCCACCAG GTCGCAGGCA 
CAGACCCCAG TTTTAAGCCC TTCCCTTCCT GATATGTCTC TGTCCACACA TCCATCTGTT 
CAGGCAGGTG GGTGGCGAAA GCTCCCGCCT AATCTCAGCC CCACTATCGA GTATAATGAC 
GGAAGGCCCA CAAAACGGCA TGATATTGCA CGCTCCCATT CTGAAAGTCC TTCCAGACTA 
CCAATCAACC GGGCGGGAAC CTGGAAGCGT GAACACAGCA AACATTCCTC GTCCCTTCCT 
CGAGTGAGTA CTTGGAGAAG AACTGGAAGC TCATCTTCTA TTCTTTCTGC TTCATCAGAG 
TCCAGTGAAA AAGCAAAAAG TGAGGATGAA AGGCATGTGA GCTCCATGCC AGCACCCAGA 
CAGATGAAGG AAAACCAGGT GCCCACCAAA GGAACATGGA GGAAAATCAA GGAAAGTGAC 
ATTTCTCCCA CAGGCATGGC TTCTCAGAGC GCTTCCTCAG GTGCTGCCAG TGGTGCTGAA 
TCCAAGCCTC TGATCTATCA GATGGCACCT CCTGTCTCTA AAACAGAGGA TGTTTGGGTG 
AGAATTGAGG ACTGCCCCAT TAACAACCCT AGATCTGGAC GGTCCCCCAC AGGCAACACC 
CCCCCAGTGA TTGACAGTGT TTCAGAGAAG GGAAGTTCAA GCATTAAAGA TTCAAAAGAC 
ACCCATGGGA AACAGAGTGT GGGCAGTGGC AGTCCTGTGC AAACCGTGGG TCTGGAAACC 
CGCCTCAACT CCTTTGTTCA GGTAGAGGCC CCAGAACAGA AAGGAACTGA GGCAAAACCA 
GGACAGAGTA ACCCAGTCTC TATAGCAGAG ACTGCTGAGA CGTGTATAGC AGAGCGTACC 
CCTTTCAGTT CCAGTAGCTC CAGCAAGCAC AGCTCACCTA GCGGGACTGT TGCTGCCAGA 
GTGACACCTT TTAATTACAA CCCTAGCCCT AGGAAGAGCA GCGCAGACAG CACTTCAGCC 
CGGCCGTCTC AGATCCCTAC GCCAGTGAGC ACCAACACGA AGAAGAGAGA TTCGAAGACT 
GACAGCACAG AATCCAGTGG AGCCCAAAGT CCTAAACGCC ATTCCGGGTC TTACCTCGTG 
ACGTCTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250286.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106146.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X16, mRNA.

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CloneID	OMu79754
Clone ID Related Accession (Same CDS sequence)	XM_030250287.1 , XM_030250286.1 , NM_001360979.1 , XM_017317804.1
Accession Version	XM_030250286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGGGGA CTGCAGGCCA TTGCAGAGTT ATTGCAGGTG 
GACTGTGAGA TGTATGGGCT TACTAATGAC CACTACAGTG TTACTTTAAG ACGGTATGCT 
GGAATGGCTT TGACAAACTT GACCTTTGGA GATGTTGCCA ACAAGGCTAC GCTGTGTTCT 
ATGAAAGGCT GCATGAGAGC ACTTGTGGCC CAGTTAAAAT CTGAGAGTGA AGACTTACAG 
CAGGTTATTG CAAGTGTTTT GAGGAATTTG TCTTGGCGAG CAGATGTAAA TAGCAAAAAG 
ACGTTGAGAG AAGTTGGAAG TGTGAAAGCA TTGATGGAAT GTGCTTTGGA AGTTAAAAAG 
GAATCAACCC TCAAAAGCGT TTTGAGTGCC TTATGGAACC TGTCTGCACA CTGCACTGAG 
AATAAGGCTG ACATCTGTGC TGTGGATGGA GCACTGGCAT TTCTGGTTGG CACCCTCACT 
TACCGGAGCC AGACAAATAC TTTAGCCATT ATTGAAAGTG GAGGTGGGAT ATTACGGAAT 
GTGTCCAGCT TGATAGCTAC AAACGAAGAC CACAGGCAAA TCCTAAGAGA GAACAATTGC 
CTACAAACTT TATTACAGCA CTTGAAATCT CACAGCTTGA CAATAGTCAG TAATGCATGT 
GGAACTTTGT GGAATCTCTC AGCAAGAAAT CCTAAAGACC AGGAAGCCTT GTGGGACATG 
GGGGCAGTGA GCATGCTCAA GAACCTCATT CATTCCAAGC ACAAAATGAT TGCCATGGGA 
AGTGCAGCAG CTTTAAGGAA TCTCATGGCA AACAGACCTG CAAAGTATAA GGATGCCAAT 
ATCATGTCTC CCGGCTCAAG TCTGCCATCC CTTCACGTTA GGAAACAGAA AGCTCTAGAA 
GCTGAGCTAG ATGCTCAGCA TTTATCAGAA ACCTTCGACA ACATTGACAA CCTAAGTCCC 
AAGGCCTCTC ACCGGAGTAA GCAGAGACAC AAGCAGAATC TTTATGGTGA CTATGCTTTT 
GACGCCAATC GACATGATGA TAGTAGGTCA GACAATTTCA ATACTGGAAA CATGACTGTT 
CTTTCACCAT ATTTAAATAC TACGGTATTG CCCAGCTCTT CTTCCTCAAG GGGAAGTTTA 
GACAGTTCTC GTTCTGAGAA AGACAGAAGT TTGGAGAGAG AGCGAGGTAT TGGCCTCAGT 
GCTTACCATC CAACAACAGA AAATGCAGGA ACCTCATCAA AACGAGGTCT GCAGATCACT 
ACCACTGCAG CCCAGATAGC CAAAGTTATG GAAGAAGTAT CAGCCATTCA TACCTCCCAG 
GACGACAGAA GTTCTGCTTC TACCACCGAG TTCCATTGTG TGGCAGACGA CAGGAGTGCG 
GCACGAAGAA GCTCTGCCTC CCACACACAC TCAAACACAT ACAACTTCAC TAAGTCGGAA 
AATTCAAATA GGACATGCTC TATGCCTTAT GCCAAAGTGG AATATAAACG ATCTTCAAAT 
GACAGTTTAA ATAGTGTCAC TAGTAGTGAT GGATATGGTA AAAGAGGCCA AATGAAACCC 
TCAGTTGAAT CCTATTCTGA AGATGATGAA AGTAAATTTT GCAGTTATGG TCAGTATCCA 
GCTGACCTAG CCCATAAGAT ACACAGTGCA AATCATATGG ATGATAATGA TGGAGAACTG 
GATACACCAA TAAATTACAG TCTTAAATAT TCAGATGAGC AGTTGAACTC AGGAAGGCAG 
AGTCCCTCAC AGAATGAAAG GTGGGCAAGA CCAAAGCATG TGATAGAAGA TGAAATAAAG 
CAAAACGAGC AAAGACAAGC AAGAAGCCAG AACACCAGTT ATCCTGTCTA TTCTGAGAAT 
ACCGATGACA AACACCTCAA ATTCCAACCA CATTTTGGAC AACAAGAATG TGTTTCCCCA 
TATAGGTCAA GGGGAACCAG TGGTTCAGAA ACAAATCGAA TGGGTTCTAG TCATGCAATT 
AATCAAAATG TAAACCAGTC TCTGTGTCAG GAAGATGATT ATGAAGATGA TAAACCTACC 
AACTACAGTG AACGTTATTC TGAGGAAGAA CAACATGAAG AAGAAGAAGA GAGACCGACA 
AATTATAGCA TAAAATATAA TGAAGAGAAA CATCATGTGG ATCAGCCTAT TGATTATAGT 
TTAAAATATG CCACTGACAT TTCTTCCTCA CAAAAACCAT CATTTTCATT CTCAAAGAAT 
TCATCAGCAC AAAGCACTAA ACCTGAACAT CTCTCTCCAA GCAGCGAGAA TACAGCTGTA 
CCTCCATCTA ATGCCAAAAG GCAGAATCAG CTGCGTCCAA GTTCAGCACA AAGAAATGGC 
CAGACTCAAA AAGGCACTAC TTGCAAAGTC CCCTCCATCA ACCAAGAAAC AATACAGACT 
TACTGCGTAG AAGACACCCC AATATGTTTT TCAAGGTGCA GTTCATTATC ATCACTGTCA 
TCAGCTGACG ATGAAATAGG ATGTGATCAG ACAACACAGG AAGCAGATTC TGCTAATACT 
CTGCAGACAG CAGAAGTAAA AGAGAATGAT GTAACTCGGT CAGCTGAAGA TCCTGCAACT 
GAAGTTCCAG CAGTGTCCCA GAATGCTAGA GCCAAACCCA GCCGACTCCA GGCTTCTGGC 
TTATCTTCAG AATCAACCAG GCATAATAAA GCTGTTGAGT TTTCTTCAGG AGCCAAGTCT 
CCCTCCAAAA GTGGTGCTCA GACACCCAAA AGTCCCCCAG AACACTATGT CCAGGAGACT 
CCGCTCGTAT TCAGCAGGTG TACTTCTGTC AGCTCCCTTG ACAGTTTTGA GAGTCGCTCC 
ATTGCCAGCT CTGTTCAGAG TGAGCCATGT AGTGGAATGG TGAGTGGCAT CATAAGCCCC 
AGTGACCTTC CAGATAGTCC TGGGCAGACC ATGCCACCAA GCAGAAGCAA AACCCCTCCA 
CCTCCTCCAC AGACAGTGCA GGCCAAGAGA GAGGTGCCAA AAAGTAAAGT CCCTGCTGCT 
GAGAAGAGAG AGAGTGGGCC TAAGCAGACT GCTGTAAATG CTGCCGTGCA GAGGGTGCAG 
GTCCTTCCAG ACGTGGATAC TTTGTTACAC TTCGCCACAG AAAGTACTCC AGACGGGTTT 
TCTTGTTCCT CCAGCCTAAG TGCTCTGAGC CTGGATGAGC CATTTATACA GAAAGATGTA 
GAATTAAGAA TCATGCCTCC AGTTCAGGAA AACGACAATG GGAATGAAAC TGAATCAGAA 
CAGCCTGAGG AATCAAATGA AAACCAGGAT AAAGAGGTAG AAAAGCCTGA CTCTGAAAAA 
GACTTATTAG ATGATTCTGA TGACGATGAT ATTGAAATAT TAGAAGAATG TATTATTTCA 
GCCATGCCAA CAAAGTCATC ACGCAAAGCC AAAAAACTAG CCCAGACTGC TTCAAAATTA 
CCTCCACCTG TGGCAAGGAA ACCAAGTCAG CTACCTGTGT ATAAACTTCT GCCAGCACAG 
AATAGGCTGC AGGCACAAAA ACATGTTAGC TTTACACCAG GGGATGATGT GCCCCGGGTG 
TACTGTGTAG AAGGGACACC TATAAACTTT TCCACAGCAA CGTCTCTAAG TGATCTGACA 
ATAGAGTCCC CTCCAAATGA ATTGGCTACT GGAGATGGGG TCAGAGCGGG TATACAGTCA 
GGTGAATTTG AAAAACGAGA TACCATTCCT ACAGAAGGCA GAAGTACAGA TGATGCTCAG 
CGAGGAAAAA TCTCATCTAT AGTTACACCA GACCTGGATG ACAACAAAGC AGAGGAAGGA 
GATATTCTTG CAGAATGTAT CAATTCTGCT ATGCCCAAAG GAAAAAGCCA CAAGCCTTTC 
CGAGTGAAAA AGATAATGGA CCAAGTCCAA CAAGCATCCT CGACTTCATC TGGAGCTAAC 
AAAAATCAAG TAGACACTAA GAAAAAGAAG CCTACTTCAC CAGTAAAGCC CATGCCACAA 
AATACTGAAT ATAGAACGCG TGTGAGAAAG AATACAGACT CAAAAGTTAA TGTAAATACT 
GAAGAAACTT TCTCAGACAA CAAAGACTCA AAGAAACCAA GCTTACAAAC CAATGCCAAG 
GCCTTCAATG AAAAGCTACC TAACAATGAA GACAGAGTGC GGGGGAGCTT CGCCTTGGAC 
TCACCGCATC ACTACACCCC TATTGAGGGG ACGCCGTACT GCTTTTCCCG AAATGACTCC 
TTGAGTTCTC TGGATTTTGA TGATGACGAT GTTGACCTTT CCAGGGAAAA GGCCGAGTTA 
AGAAAGGGCA AAGAAAGCAA GGATTCCGAA GCCAAAGTTA CCTGCCGCCC AGAACCAAAC 
TCAAGCCAGC AGGCAGCTAG TAAGTCACAA GCCAGTATAA AACATCCAGC AAACAGAGCA 
CAGTCCAAAC CAGTGCTGCA GAAACAGCCC ACTTTCCCCC AGTCCTCCAA AGACGGACCA 
GATAGAGGGG CAGCAACTGA CGAAAAACTG CAGAATTTTG CTATTGAAAA TACTCCAGTT 
TGCTTTTCTC GAAATTCCTC TCTGAGTTCC CTTAGTGACA TTGACCAGGA AAACAACAAT 
AACAAAGAAA GTGAACCAAT CAAAGAAGCT GAACCTGCCA ACTCACAAGG AGAGCCCAGT 
AAGCCTCAGG CATCCGGGTA TGCTCCCAAG TCCTTCCACG TCGAAGACAC CCCTGTCTGT 
TTCTCAAGAA ACAGCTCTCT CAGTTCTCTT AGCATTGACT CTGAGGACGA CCTGTTACAG 
GAGTGTATAA GTTCTGCCAT GCCAAAAAAG AAAAGGCCTT CAAGACTCAA GAGTGAGAGC 
GAAAAGCAGA GCCCTAGAAA AGTGGGTGGC ATATTAGCTG AAGACCTGAC GCTTGATTTG 
AAAGATCTAC AGAGGCCAGA TTCAGAACAC GCTTTCTCCC CCGACTCAGA AAATTTTGAC 
TGGAAAGCTA TTCAGGAAGG CGCAAACTCC ATAGTAAGTA GTTTGCACCA AGCTGCTGCA 
GCCGCCGCGT GCTTATCTAG ACAAGCGTCA TCCGACTCAG ATTCCATTCT GTCACTAAAG 
TCCGGCATTT CTCTGGGATC GCCTTTTCAT CTTACACCTG ATCAAGAGGA AAAGCCATTC 
ACAAGCAATA AAGGCCCAAG AATTCTCAAA CCTGGAGAGA AAAGCACATT AGAAGCAAAA 
AAAATAGAAT CTGAAAACAA AGGAATCAAA GGCGGGAAAA AGGTTTATAA AAGCTTGATT 
ACGGGAAAGA TTCGCTCCAA TTCAGAAATT TCCAGCCAAA TGAAACAACC CCTCCCGACA 
AACATGCCTT CAATCTCAAG AGGCAGGACG ATGATTCACA TCCCAGGGCT TCGGAATAGC 
TCCTCTAGTA CAAGCCCTGT CTCTAAGAAA GGCCCACCCC TCAAGACTCC AGCCTCTAAA 
AGCCCCAGTG AAGGGCCGGG AGCTACCACT TCTCCTCGAG GAACTAAGCC AGCAGGAAAG 
TCAGAGCTTA GCCCTATCAC CAGGCAAACT TCCCAAATCA GTGGGTCAAA TAAGGGGTCT 
TCTAGATCAG GATCTAGAGA CTCCACTCCC TCAAGACCTA CACAGCAACC ATTAAGTAGG 
CCAATGCAGT CTCCAGGGCG AAACTCAATT TCCCCTGGTA GAAATGGAAT AAGCCCTCCT 
AACAAACTGT CTCAGCTGCC CAGAACATCA TCTCCCAGTA CTGCTTCAAC TAAGTCCTCC 
GGTTCTGGGA AAATGTCATA TACATCCCCA GGTAGACAGC TGAGCCAACA AAATCTTACC 
AAACAAGCAA GTTTATCCAA GAATGCCAGC AGTATCCCCA GAAGTGAGTC GGCATCTAAA 
GGACTGAATC AGATGAGTAA CGGCAATGGG TCAAATAAAA AGGTAGAACT TTCTAGAATG 
TCTTCAACTA AATCAAGTGG AAGTGAATCA GACAGATCAG AAAGGCCTGC ATTAGTACGC 
CAGTCTACTT TCATCAAAGA AGCCCCAAGC CCAACCCTGA GGAGGAAACT GGAGGAATCT 
GCCTCATTTG AATCCCTTTC TCCATCTTCT AGACCAGATT CTCCCACCAG GTCGCAGGCA 
CAGACCCCAG TTTTAAGCCC TTCCCTTCCT GATATGTCTC TGTCCACACA TCCATCTGTT 
CAGGCAGGTG GGTGGCGAAA GCTCCCGCCT AATCTCAGCC CCACTATCGA GTATAATGAC 
GGAAGGCCCA CAAAACGGCA TGATATTGCA CGCTCCCATT CTGAAAGTCC TTCCAGACTA 
CCAATCAACC GGGCGGGAAC CTGGAAGCGT GAACACAGCA AACATTCCTC GTCCCTTCCT 
CGAGTGAGTA CTTGGAGAAG AACTGGAAGC TCATCTTCTA TTCTTTCTGC TTCATCAGAG 
TCCAGTGAAA AAGCAAAAAG TGAGGATGAA AGGCATGTGA GCTCCATGCC AGCACCCAGA 
CAGATGAAGG AAAACCAGGT GCCCACCAAA GGAACATGGA GGAAAATCAA GGAAAGTGAC 
ATTTCTCCCA CAGGCATGGC TTCTCAGAGC GCTTCCTCAG GTGCTGCCAG TGGTGCTGAA 
TCCAAGCCTC TGATCTATCA GATGGCACCT CCTGTCTCTA AAACAGAGGA TGTTTGGGTG 
AGAATTGAGG ACTGCCCCAT TAACAACCCT AGATCTGGAC GGTCCCCCAC AGGCAACACC 
CCCCCAGTGA TTGACAGTGT TTCAGAGAAG GGAAGTTCAA GCATTAAAGA TTCAAAAGAC 
ACCCATGGGA AACAGAGTGT GGGCAGTGGC AGTCCTGTGC AAACCGTGGG TCTGGAAACC 
CGCCTCAACT CCTTTGTTCA GGTAGAGGCC CCAGAACAGA AAGGAACTGA GGCAAAACCA 
GGACAGAGTA ACCCAGTCTC TATAGCAGAG ACTGCTGAGA CGTGTATAGC AGAGCGTACC 
CCTTTCAGTT CCAGTAGCTC CAGCAAGCAC AGCTCACCTA GCGGGACTGT TGCTGCCAGA 
GTGACACCTT TTAATTACAA CCCTAGCCCT AGGAAGAGCA GCGCAGACAG CACTTCAGCC 
CGGCCGTCTC AGATCCCTAC GCCAGTGAGC ACCAACACGA AGAAGAGAGA TTCGAAGACT 
GACAGCACAG AATCCAGTGG AGCCCAAAGT CCTAAACGCC ATTCCGGGTC TTACCTCGTG 
ACGTCTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106146.1
CDS	725..9076
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250286.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250286.1

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGGGGA CTGCAGGCCA TTGCAGAGTT ATTGCAGGTG 
GACTGTGAGA TGTATGGGCT TACTAATGAC CACTACAGTG TTACTTTAAG ACGGTATGCT 
GGAATGGCTT TGACAAACTT GACCTTTGGA GATGTTGCCA ACAAGGCTAC GCTGTGTTCT 
ATGAAAGGCT GCATGAGAGC ACTTGTGGCC CAGTTAAAAT CTGAGAGTGA AGACTTACAG 
CAGGTTATTG CAAGTGTTTT GAGGAATTTG TCTTGGCGAG CAGATGTAAA TAGCAAAAAG 
ACGTTGAGAG AAGTTGGAAG TGTGAAAGCA TTGATGGAAT GTGCTTTGGA AGTTAAAAAG 
GAATCAACCC TCAAAAGCGT TTTGAGTGCC TTATGGAACC TGTCTGCACA CTGCACTGAG 
AATAAGGCTG ACATCTGTGC TGTGGATGGA GCACTGGCAT TTCTGGTTGG CACCCTCACT 
TACCGGAGCC AGACAAATAC TTTAGCCATT ATTGAAAGTG GAGGTGGGAT ATTACGGAAT 
GTGTCCAGCT TGATAGCTAC AAACGAAGAC CACAGGCAAA TCCTAAGAGA GAACAATTGC 
CTACAAACTT TATTACAGCA CTTGAAATCT CACAGCTTGA CAATAGTCAG TAATGCATGT 
GGAACTTTGT GGAATCTCTC AGCAAGAAAT CCTAAAGACC AGGAAGCCTT GTGGGACATG 
GGGGCAGTGA GCATGCTCAA GAACCTCATT CATTCCAAGC ACAAAATGAT TGCCATGGGA 
AGTGCAGCAG CTTTAAGGAA TCTCATGGCA AACAGACCTG CAAAGTATAA GGATGCCAAT 
ATCATGTCTC CCGGCTCAAG TCTGCCATCC CTTCACGTTA GGAAACAGAA AGCTCTAGAA 
GCTGAGCTAG ATGCTCAGCA TTTATCAGAA ACCTTCGACA ACATTGACAA CCTAAGTCCC 
AAGGCCTCTC ACCGGAGTAA GCAGAGACAC AAGCAGAATC TTTATGGTGA CTATGCTTTT 
GACGCCAATC GACATGATGA TAGTAGGTCA GACAATTTCA ATACTGGAAA CATGACTGTT 
CTTTCACCAT ATTTAAATAC TACGGTATTG CCCAGCTCTT CTTCCTCAAG GGGAAGTTTA 
GACAGTTCTC GTTCTGAGAA AGACAGAAGT TTGGAGAGAG AGCGAGGTAT TGGCCTCAGT 
GCTTACCATC CAACAACAGA AAATGCAGGA ACCTCATCAA AACGAGGTCT GCAGATCACT 
ACCACTGCAG CCCAGATAGC CAAAGTTATG GAAGAAGTAT CAGCCATTCA TACCTCCCAG 
GACGACAGAA GTTCTGCTTC TACCACCGAG TTCCATTGTG TGGCAGACGA CAGGAGTGCG 
GCACGAAGAA GCTCTGCCTC CCACACACAC TCAAACACAT ACAACTTCAC TAAGTCGGAA 
AATTCAAATA GGACATGCTC TATGCCTTAT GCCAAAGTGG AATATAAACG ATCTTCAAAT 
GACAGTTTAA ATAGTGTCAC TAGTAGTGAT GGATATGGTA AAAGAGGCCA AATGAAACCC 
TCAGTTGAAT CCTATTCTGA AGATGATGAA AGTAAATTTT GCAGTTATGG TCAGTATCCA 
GCTGACCTAG CCCATAAGAT ACACAGTGCA AATCATATGG ATGATAATGA TGGAGAACTG 
GATACACCAA TAAATTACAG TCTTAAATAT TCAGATGAGC AGTTGAACTC AGGAAGGCAG 
AGTCCCTCAC AGAATGAAAG GTGGGCAAGA CCAAAGCATG TGATAGAAGA TGAAATAAAG 
CAAAACGAGC AAAGACAAGC AAGAAGCCAG AACACCAGTT ATCCTGTCTA TTCTGAGAAT 
ACCGATGACA AACACCTCAA ATTCCAACCA CATTTTGGAC AACAAGAATG TGTTTCCCCA 
TATAGGTCAA GGGGAACCAG TGGTTCAGAA ACAAATCGAA TGGGTTCTAG TCATGCAATT 
AATCAAAATG TAAACCAGTC TCTGTGTCAG GAAGATGATT ATGAAGATGA TAAACCTACC 
AACTACAGTG AACGTTATTC TGAGGAAGAA CAACATGAAG AAGAAGAAGA GAGACCGACA 
AATTATAGCA TAAAATATAA TGAAGAGAAA CATCATGTGG ATCAGCCTAT TGATTATAGT 
TTAAAATATG CCACTGACAT TTCTTCCTCA CAAAAACCAT CATTTTCATT CTCAAAGAAT 
TCATCAGCAC AAAGCACTAA ACCTGAACAT CTCTCTCCAA GCAGCGAGAA TACAGCTGTA 
CCTCCATCTA ATGCCAAAAG GCAGAATCAG CTGCGTCCAA GTTCAGCACA AAGAAATGGC 
CAGACTCAAA AAGGCACTAC TTGCAAAGTC CCCTCCATCA ACCAAGAAAC AATACAGACT 
TACTGCGTAG AAGACACCCC AATATGTTTT TCAAGGTGCA GTTCATTATC ATCACTGTCA 
TCAGCTGACG ATGAAATAGG ATGTGATCAG ACAACACAGG AAGCAGATTC TGCTAATACT 
CTGCAGACAG CAGAAGTAAA AGAGAATGAT GTAACTCGGT CAGCTGAAGA TCCTGCAACT 
GAAGTTCCAG CAGTGTCCCA GAATGCTAGA GCCAAACCCA GCCGACTCCA GGCTTCTGGC 
TTATCTTCAG AATCAACCAG GCATAATAAA GCTGTTGAGT TTTCTTCAGG AGCCAAGTCT 
CCCTCCAAAA GTGGTGCTCA GACACCCAAA AGTCCCCCAG AACACTATGT CCAGGAGACT 
CCGCTCGTAT TCAGCAGGTG TACTTCTGTC AGCTCCCTTG ACAGTTTTGA GAGTCGCTCC 
ATTGCCAGCT CTGTTCAGAG TGAGCCATGT AGTGGAATGG TGAGTGGCAT CATAAGCCCC 
AGTGACCTTC CAGATAGTCC TGGGCAGACC ATGCCACCAA GCAGAAGCAA AACCCCTCCA 
CCTCCTCCAC AGACAGTGCA GGCCAAGAGA GAGGTGCCAA AAAGTAAAGT CCCTGCTGCT 
GAGAAGAGAG AGAGTGGGCC TAAGCAGACT GCTGTAAATG CTGCCGTGCA GAGGGTGCAG 
GTCCTTCCAG ACGTGGATAC TTTGTTACAC TTCGCCACAG AAAGTACTCC AGACGGGTTT 
TCTTGTTCCT CCAGCCTAAG TGCTCTGAGC CTGGATGAGC CATTTATACA GAAAGATGTA 
GAATTAAGAA TCATGCCTCC AGTTCAGGAA AACGACAATG GGAATGAAAC TGAATCAGAA 
CAGCCTGAGG AATCAAATGA AAACCAGGAT AAAGAGGTAG AAAAGCCTGA CTCTGAAAAA 
GACTTATTAG ATGATTCTGA TGACGATGAT ATTGAAATAT TAGAAGAATG TATTATTTCA 
GCCATGCCAA CAAAGTCATC ACGCAAAGCC AAAAAACTAG CCCAGACTGC TTCAAAATTA 
CCTCCACCTG TGGCAAGGAA ACCAAGTCAG CTACCTGTGT ATAAACTTCT GCCAGCACAG 
AATAGGCTGC AGGCACAAAA ACATGTTAGC TTTACACCAG GGGATGATGT GCCCCGGGTG 
TACTGTGTAG AAGGGACACC TATAAACTTT TCCACAGCAA CGTCTCTAAG TGATCTGACA 
ATAGAGTCCC CTCCAAATGA ATTGGCTACT GGAGATGGGG TCAGAGCGGG TATACAGTCA 
GGTGAATTTG AAAAACGAGA TACCATTCCT ACAGAAGGCA GAAGTACAGA TGATGCTCAG 
CGAGGAAAAA TCTCATCTAT AGTTACACCA GACCTGGATG ACAACAAAGC AGAGGAAGGA 
GATATTCTTG CAGAATGTAT CAATTCTGCT ATGCCCAAAG GAAAAAGCCA CAAGCCTTTC 
CGAGTGAAAA AGATAATGGA CCAAGTCCAA CAAGCATCCT CGACTTCATC TGGAGCTAAC 
AAAAATCAAG TAGACACTAA GAAAAAGAAG CCTACTTCAC CAGTAAAGCC CATGCCACAA 
AATACTGAAT ATAGAACGCG TGTGAGAAAG AATACAGACT CAAAAGTTAA TGTAAATACT 
GAAGAAACTT TCTCAGACAA CAAAGACTCA AAGAAACCAA GCTTACAAAC CAATGCCAAG 
GCCTTCAATG AAAAGCTACC TAACAATGAA GACAGAGTGC GGGGGAGCTT CGCCTTGGAC 
TCACCGCATC ACTACACCCC TATTGAGGGG ACGCCGTACT GCTTTTCCCG AAATGACTCC 
TTGAGTTCTC TGGATTTTGA TGATGACGAT GTTGACCTTT CCAGGGAAAA GGCCGAGTTA 
AGAAAGGGCA AAGAAAGCAA GGATTCCGAA GCCAAAGTTA CCTGCCGCCC AGAACCAAAC 
TCAAGCCAGC AGGCAGCTAG TAAGTCACAA GCCAGTATAA AACATCCAGC AAACAGAGCA 
CAGTCCAAAC CAGTGCTGCA GAAACAGCCC ACTTTCCCCC AGTCCTCCAA AGACGGACCA 
GATAGAGGGG CAGCAACTGA CGAAAAACTG CAGAATTTTG CTATTGAAAA TACTCCAGTT 
TGCTTTTCTC GAAATTCCTC TCTGAGTTCC CTTAGTGACA TTGACCAGGA AAACAACAAT 
AACAAAGAAA GTGAACCAAT CAAAGAAGCT GAACCTGCCA ACTCACAAGG AGAGCCCAGT 
AAGCCTCAGG CATCCGGGTA TGCTCCCAAG TCCTTCCACG TCGAAGACAC CCCTGTCTGT 
TTCTCAAGAA ACAGCTCTCT CAGTTCTCTT AGCATTGACT CTGAGGACGA CCTGTTACAG 
GAGTGTATAA GTTCTGCCAT GCCAAAAAAG AAAAGGCCTT CAAGACTCAA GAGTGAGAGC 
GAAAAGCAGA GCCCTAGAAA AGTGGGTGGC ATATTAGCTG AAGACCTGAC GCTTGATTTG 
AAAGATCTAC AGAGGCCAGA TTCAGAACAC GCTTTCTCCC CCGACTCAGA AAATTTTGAC 
TGGAAAGCTA TTCAGGAAGG CGCAAACTCC ATAGTAAGTA GTTTGCACCA AGCTGCTGCA 
GCCGCCGCGT GCTTATCTAG ACAAGCGTCA TCCGACTCAG ATTCCATTCT GTCACTAAAG 
TCCGGCATTT CTCTGGGATC GCCTTTTCAT CTTACACCTG ATCAAGAGGA AAAGCCATTC 
ACAAGCAATA AAGGCCCAAG AATTCTCAAA CCTGGAGAGA AAAGCACATT AGAAGCAAAA 
AAAATAGAAT CTGAAAACAA AGGAATCAAA GGCGGGAAAA AGGTTTATAA AAGCTTGATT 
ACGGGAAAGA TTCGCTCCAA TTCAGAAATT TCCAGCCAAA TGAAACAACC CCTCCCGACA 
AACATGCCTT CAATCTCAAG AGGCAGGACG ATGATTCACA TCCCAGGGCT TCGGAATAGC 
TCCTCTAGTA CAAGCCCTGT CTCTAAGAAA GGCCCACCCC TCAAGACTCC AGCCTCTAAA 
AGCCCCAGTG AAGGGCCGGG AGCTACCACT TCTCCTCGAG GAACTAAGCC AGCAGGAAAG 
TCAGAGCTTA GCCCTATCAC CAGGCAAACT TCCCAAATCA GTGGGTCAAA TAAGGGGTCT 
TCTAGATCAG GATCTAGAGA CTCCACTCCC TCAAGACCTA CACAGCAACC ATTAAGTAGG 
CCAATGCAGT CTCCAGGGCG AAACTCAATT TCCCCTGGTA GAAATGGAAT AAGCCCTCCT 
AACAAACTGT CTCAGCTGCC CAGAACATCA TCTCCCAGTA CTGCTTCAAC TAAGTCCTCC 
GGTTCTGGGA AAATGTCATA TACATCCCCA GGTAGACAGC TGAGCCAACA AAATCTTACC 
AAACAAGCAA GTTTATCCAA GAATGCCAGC AGTATCCCCA GAAGTGAGTC GGCATCTAAA 
GGACTGAATC AGATGAGTAA CGGCAATGGG TCAAATAAAA AGGTAGAACT TTCTAGAATG 
TCTTCAACTA AATCAAGTGG AAGTGAATCA GACAGATCAG AAAGGCCTGC ATTAGTACGC 
CAGTCTACTT TCATCAAAGA AGCCCCAAGC CCAACCCTGA GGAGGAAACT GGAGGAATCT 
GCCTCATTTG AATCCCTTTC TCCATCTTCT AGACCAGATT CTCCCACCAG GTCGCAGGCA 
CAGACCCCAG TTTTAAGCCC TTCCCTTCCT GATATGTCTC TGTCCACACA TCCATCTGTT 
CAGGCAGGTG GGTGGCGAAA GCTCCCGCCT AATCTCAGCC CCACTATCGA GTATAATGAC 
GGAAGGCCCA CAAAACGGCA TGATATTGCA CGCTCCCATT CTGAAAGTCC TTCCAGACTA 
CCAATCAACC GGGCGGGAAC CTGGAAGCGT GAACACAGCA AACATTCCTC GTCCCTTCCT 
CGAGTGAGTA CTTGGAGAAG AACTGGAAGC TCATCTTCTA TTCTTTCTGC TTCATCAGAG 
TCCAGTGAAA AAGCAAAAAG TGAGGATGAA AGGCATGTGA GCTCCATGCC AGCACCCAGA 
CAGATGAAGG AAAACCAGGT GCCCACCAAA GGAACATGGA GGAAAATCAA GGAAAGTGAC 
ATTTCTCCCA CAGGCATGGC TTCTCAGAGC GCTTCCTCAG GTGCTGCCAG TGGTGCTGAA 
TCCAAGCCTC TGATCTATCA GATGGCACCT CCTGTCTCTA AAACAGAGGA TGTTTGGGTG 
AGAATTGAGG ACTGCCCCAT TAACAACCCT AGATCTGGAC GGTCCCCCAC AGGCAACACC 
CCCCCAGTGA TTGACAGTGT TTCAGAGAAG GGAAGTTCAA GCATTAAAGA TTCAAAAGAC 
ACCCATGGGA AACAGAGTGT GGGCAGTGGC AGTCCTGTGC AAACCGTGGG TCTGGAAACC 
CGCCTCAACT CCTTTGTTCA GGTAGAGGCC CCAGAACAGA AAGGAACTGA GGCAAAACCA 
GGACAGAGTA ACCCAGTCTC TATAGCAGAG ACTGCTGAGA CGTGTATAGC AGAGCGTACC 
CCTTTCAGTT CCAGTAGCTC CAGCAAGCAC AGCTCACCTA GCGGGACTGT TGCTGCCAGA 
GTGACACCTT TTAATTACAA CCCTAGCCCT AGGAAGAGCA GCGCAGACAG CACTTCAGCC 
CGGCCGTCTC AGATCCCTAC GCCAGTGAGC ACCAACACGA AGAAGAGAGA TTCGAAGACT 
GACAGCACAG AATCCAGTGG AGCCCAAAGT CCTAAACGCC ATTCCGGGTC TTACCTCGTG 
ACGTCTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_017317804.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173293.1 Mus musculus adenomatosis polyposis coli (Apc), transcript variant X9, mRNA.

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CloneID	OMu79754
Clone ID Related Accession (Same CDS sequence)	XM_030250287.1 , XM_030250286.1 , NM_001360979.1 , XM_017317804.1
Accession Version	XM_017317804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGGGGA CTGCAGGCCA TTGCAGAGTT ATTGCAGGTG 
GACTGTGAGA TGTATGGGCT TACTAATGAC CACTACAGTG TTACTTTAAG ACGGTATGCT 
GGAATGGCTT TGACAAACTT GACCTTTGGA GATGTTGCCA ACAAGGCTAC GCTGTGTTCT 
ATGAAAGGCT GCATGAGAGC ACTTGTGGCC CAGTTAAAAT CTGAGAGTGA AGACTTACAG 
CAGGTTATTG CAAGTGTTTT GAGGAATTTG TCTTGGCGAG CAGATGTAAA TAGCAAAAAG 
ACGTTGAGAG AAGTTGGAAG TGTGAAAGCA TTGATGGAAT GTGCTTTGGA AGTTAAAAAG 
GAATCAACCC TCAAAAGCGT TTTGAGTGCC TTATGGAACC TGTCTGCACA CTGCACTGAG 
AATAAGGCTG ACATCTGTGC TGTGGATGGA GCACTGGCAT TTCTGGTTGG CACCCTCACT 
TACCGGAGCC AGACAAATAC TTTAGCCATT ATTGAAAGTG GAGGTGGGAT ATTACGGAAT 
GTGTCCAGCT TGATAGCTAC AAACGAAGAC CACAGGCAAA TCCTAAGAGA GAACAATTGC 
CTACAAACTT TATTACAGCA CTTGAAATCT CACAGCTTGA CAATAGTCAG TAATGCATGT 
GGAACTTTGT GGAATCTCTC AGCAAGAAAT CCTAAAGACC AGGAAGCCTT GTGGGACATG 
GGGGCAGTGA GCATGCTCAA GAACCTCATT CATTCCAAGC ACAAAATGAT TGCCATGGGA 
AGTGCAGCAG CTTTAAGGAA TCTCATGGCA AACAGACCTG CAAAGTATAA GGATGCCAAT 
ATCATGTCTC CCGGCTCAAG TCTGCCATCC CTTCACGTTA GGAAACAGAA AGCTCTAGAA 
GCTGAGCTAG ATGCTCAGCA TTTATCAGAA ACCTTCGACA ACATTGACAA CCTAAGTCCC 
AAGGCCTCTC ACCGGAGTAA GCAGAGACAC AAGCAGAATC TTTATGGTGA CTATGCTTTT 
GACGCCAATC GACATGATGA TAGTAGGTCA GACAATTTCA ATACTGGAAA CATGACTGTT 
CTTTCACCAT ATTTAAATAC TACGGTATTG CCCAGCTCTT CTTCCTCAAG GGGAAGTTTA 
GACAGTTCTC GTTCTGAGAA AGACAGAAGT TTGGAGAGAG AGCGAGGTAT TGGCCTCAGT 
GCTTACCATC CAACAACAGA AAATGCAGGA ACCTCATCAA AACGAGGTCT GCAGATCACT 
ACCACTGCAG CCCAGATAGC CAAAGTTATG GAAGAAGTAT CAGCCATTCA TACCTCCCAG 
GACGACAGAA GTTCTGCTTC TACCACCGAG TTCCATTGTG TGGCAGACGA CAGGAGTGCG 
GCACGAAGAA GCTCTGCCTC CCACACACAC TCAAACACAT ACAACTTCAC TAAGTCGGAA 
AATTCAAATA GGACATGCTC TATGCCTTAT GCCAAAGTGG AATATAAACG ATCTTCAAAT 
GACAGTTTAA ATAGTGTCAC TAGTAGTGAT GGATATGGTA AAAGAGGCCA AATGAAACCC 
TCAGTTGAAT CCTATTCTGA AGATGATGAA AGTAAATTTT GCAGTTATGG TCAGTATCCA 
GCTGACCTAG CCCATAAGAT ACACAGTGCA AATCATATGG ATGATAATGA TGGAGAACTG 
GATACACCAA TAAATTACAG TCTTAAATAT TCAGATGAGC AGTTGAACTC AGGAAGGCAG 
AGTCCCTCAC AGAATGAAAG GTGGGCAAGA CCAAAGCATG TGATAGAAGA TGAAATAAAG 
CAAAACGAGC AAAGACAAGC AAGAAGCCAG AACACCAGTT ATCCTGTCTA TTCTGAGAAT 
ACCGATGACA AACACCTCAA ATTCCAACCA CATTTTGGAC AACAAGAATG TGTTTCCCCA 
TATAGGTCAA GGGGAACCAG TGGTTCAGAA ACAAATCGAA TGGGTTCTAG TCATGCAATT 
AATCAAAATG TAAACCAGTC TCTGTGTCAG GAAGATGATT ATGAAGATGA TAAACCTACC 
AACTACAGTG AACGTTATTC TGAGGAAGAA CAACATGAAG AAGAAGAAGA GAGACCGACA 
AATTATAGCA TAAAATATAA TGAAGAGAAA CATCATGTGG ATCAGCCTAT TGATTATAGT 
TTAAAATATG CCACTGACAT TTCTTCCTCA CAAAAACCAT CATTTTCATT CTCAAAGAAT 
TCATCAGCAC AAAGCACTAA ACCTGAACAT CTCTCTCCAA GCAGCGAGAA TACAGCTGTA 
CCTCCATCTA ATGCCAAAAG GCAGAATCAG CTGCGTCCAA GTTCAGCACA AAGAAATGGC 
CAGACTCAAA AAGGCACTAC TTGCAAAGTC CCCTCCATCA ACCAAGAAAC AATACAGACT 
TACTGCGTAG AAGACACCCC AATATGTTTT TCAAGGTGCA GTTCATTATC ATCACTGTCA 
TCAGCTGACG ATGAAATAGG ATGTGATCAG ACAACACAGG AAGCAGATTC TGCTAATACT 
CTGCAGACAG CAGAAGTAAA AGAGAATGAT GTAACTCGGT CAGCTGAAGA TCCTGCAACT 
GAAGTTCCAG CAGTGTCCCA GAATGCTAGA GCCAAACCCA GCCGACTCCA GGCTTCTGGC 
TTATCTTCAG AATCAACCAG GCATAATAAA GCTGTTGAGT TTTCTTCAGG AGCCAAGTCT 
CCCTCCAAAA GTGGTGCTCA GACACCCAAA AGTCCCCCAG AACACTATGT CCAGGAGACT 
CCGCTCGTAT TCAGCAGGTG TACTTCTGTC AGCTCCCTTG ACAGTTTTGA GAGTCGCTCC 
ATTGCCAGCT CTGTTCAGAG TGAGCCATGT AGTGGAATGG TGAGTGGCAT CATAAGCCCC 
AGTGACCTTC CAGATAGTCC TGGGCAGACC ATGCCACCAA GCAGAAGCAA AACCCCTCCA 
CCTCCTCCAC AGACAGTGCA GGCCAAGAGA GAGGTGCCAA AAAGTAAAGT CCCTGCTGCT 
GAGAAGAGAG AGAGTGGGCC TAAGCAGACT GCTGTAAATG CTGCCGTGCA GAGGGTGCAG 
GTCCTTCCAG ACGTGGATAC TTTGTTACAC TTCGCCACAG AAAGTACTCC AGACGGGTTT 
TCTTGTTCCT CCAGCCTAAG TGCTCTGAGC CTGGATGAGC CATTTATACA GAAAGATGTA 
GAATTAAGAA TCATGCCTCC AGTTCAGGAA AACGACAATG GGAATGAAAC TGAATCAGAA 
CAGCCTGAGG AATCAAATGA AAACCAGGAT AAAGAGGTAG AAAAGCCTGA CTCTGAAAAA 
GACTTATTAG ATGATTCTGA TGACGATGAT ATTGAAATAT TAGAAGAATG TATTATTTCA 
GCCATGCCAA CAAAGTCATC ACGCAAAGCC AAAAAACTAG CCCAGACTGC TTCAAAATTA 
CCTCCACCTG TGGCAAGGAA ACCAAGTCAG CTACCTGTGT ATAAACTTCT GCCAGCACAG 
AATAGGCTGC AGGCACAAAA ACATGTTAGC TTTACACCAG GGGATGATGT GCCCCGGGTG 
TACTGTGTAG AAGGGACACC TATAAACTTT TCCACAGCAA CGTCTCTAAG TGATCTGACA 
ATAGAGTCCC CTCCAAATGA ATTGGCTACT GGAGATGGGG TCAGAGCGGG TATACAGTCA 
GGTGAATTTG AAAAACGAGA TACCATTCCT ACAGAAGGCA GAAGTACAGA TGATGCTCAG 
CGAGGAAAAA TCTCATCTAT AGTTACACCA GACCTGGATG ACAACAAAGC AGAGGAAGGA 
GATATTCTTG CAGAATGTAT CAATTCTGCT ATGCCCAAAG GAAAAAGCCA CAAGCCTTTC 
CGAGTGAAAA AGATAATGGA CCAAGTCCAA CAAGCATCCT CGACTTCATC TGGAGCTAAC 
AAAAATCAAG TAGACACTAA GAAAAAGAAG CCTACTTCAC CAGTAAAGCC CATGCCACAA 
AATACTGAAT ATAGAACGCG TGTGAGAAAG AATACAGACT CAAAAGTTAA TGTAAATACT 
GAAGAAACTT TCTCAGACAA CAAAGACTCA AAGAAACCAA GCTTACAAAC CAATGCCAAG 
GCCTTCAATG AAAAGCTACC TAACAATGAA GACAGAGTGC GGGGGAGCTT CGCCTTGGAC 
TCACCGCATC ACTACACCCC TATTGAGGGG ACGCCGTACT GCTTTTCCCG AAATGACTCC 
TTGAGTTCTC TGGATTTTGA TGATGACGAT GTTGACCTTT CCAGGGAAAA GGCCGAGTTA 
AGAAAGGGCA AAGAAAGCAA GGATTCCGAA GCCAAAGTTA CCTGCCGCCC AGAACCAAAC 
TCAAGCCAGC AGGCAGCTAG TAAGTCACAA GCCAGTATAA AACATCCAGC AAACAGAGCA 
CAGTCCAAAC CAGTGCTGCA GAAACAGCCC ACTTTCCCCC AGTCCTCCAA AGACGGACCA 
GATAGAGGGG CAGCAACTGA CGAAAAACTG CAGAATTTTG CTATTGAAAA TACTCCAGTT 
TGCTTTTCTC GAAATTCCTC TCTGAGTTCC CTTAGTGACA TTGACCAGGA AAACAACAAT 
AACAAAGAAA GTGAACCAAT CAAAGAAGCT GAACCTGCCA ACTCACAAGG AGAGCCCAGT 
AAGCCTCAGG CATCCGGGTA TGCTCCCAAG TCCTTCCACG TCGAAGACAC CCCTGTCTGT 
TTCTCAAGAA ACAGCTCTCT CAGTTCTCTT AGCATTGACT CTGAGGACGA CCTGTTACAG 
GAGTGTATAA GTTCTGCCAT GCCAAAAAAG AAAAGGCCTT CAAGACTCAA GAGTGAGAGC 
GAAAAGCAGA GCCCTAGAAA AGTGGGTGGC ATATTAGCTG AAGACCTGAC GCTTGATTTG 
AAAGATCTAC AGAGGCCAGA TTCAGAACAC GCTTTCTCCC CCGACTCAGA AAATTTTGAC 
TGGAAAGCTA TTCAGGAAGG CGCAAACTCC ATAGTAAGTA GTTTGCACCA AGCTGCTGCA 
GCCGCCGCGT GCTTATCTAG ACAAGCGTCA TCCGACTCAG ATTCCATTCT GTCACTAAAG 
TCCGGCATTT CTCTGGGATC GCCTTTTCAT CTTACACCTG ATCAAGAGGA AAAGCCATTC 
ACAAGCAATA AAGGCCCAAG AATTCTCAAA CCTGGAGAGA AAAGCACATT AGAAGCAAAA 
AAAATAGAAT CTGAAAACAA AGGAATCAAA GGCGGGAAAA AGGTTTATAA AAGCTTGATT 
ACGGGAAAGA TTCGCTCCAA TTCAGAAATT TCCAGCCAAA TGAAACAACC CCTCCCGACA 
AACATGCCTT CAATCTCAAG AGGCAGGACG ATGATTCACA TCCCAGGGCT TCGGAATAGC 
TCCTCTAGTA CAAGCCCTGT CTCTAAGAAA GGCCCACCCC TCAAGACTCC AGCCTCTAAA 
AGCCCCAGTG AAGGGCCGGG AGCTACCACT TCTCCTCGAG GAACTAAGCC AGCAGGAAAG 
TCAGAGCTTA GCCCTATCAC CAGGCAAACT TCCCAAATCA GTGGGTCAAA TAAGGGGTCT 
TCTAGATCAG GATCTAGAGA CTCCACTCCC TCAAGACCTA CACAGCAACC ATTAAGTAGG 
CCAATGCAGT CTCCAGGGCG AAACTCAATT TCCCCTGGTA GAAATGGAAT AAGCCCTCCT 
AACAAACTGT CTCAGCTGCC CAGAACATCA TCTCCCAGTA CTGCTTCAAC TAAGTCCTCC 
GGTTCTGGGA AAATGTCATA TACATCCCCA GGTAGACAGC TGAGCCAACA AAATCTTACC 
AAACAAGCAA GTTTATCCAA GAATGCCAGC AGTATCCCCA GAAGTGAGTC GGCATCTAAA 
GGACTGAATC AGATGAGTAA CGGCAATGGG TCAAATAAAA AGGTAGAACT TTCTAGAATG 
TCTTCAACTA AATCAAGTGG AAGTGAATCA GACAGATCAG AAAGGCCTGC ATTAGTACGC 
CAGTCTACTT TCATCAAAGA AGCCCCAAGC CCAACCCTGA GGAGGAAACT GGAGGAATCT 
GCCTCATTTG AATCCCTTTC TCCATCTTCT AGACCAGATT CTCCCACCAG GTCGCAGGCA 
CAGACCCCAG TTTTAAGCCC TTCCCTTCCT GATATGTCTC TGTCCACACA TCCATCTGTT 
CAGGCAGGTG GGTGGCGAAA GCTCCCGCCT AATCTCAGCC CCACTATCGA GTATAATGAC 
GGAAGGCCCA CAAAACGGCA TGATATTGCA CGCTCCCATT CTGAAAGTCC TTCCAGACTA 
CCAATCAACC GGGCGGGAAC CTGGAAGCGT GAACACAGCA AACATTCCTC GTCCCTTCCT 
CGAGTGAGTA CTTGGAGAAG AACTGGAAGC TCATCTTCTA TTCTTTCTGC TTCATCAGAG 
TCCAGTGAAA AAGCAAAAAG TGAGGATGAA AGGCATGTGA GCTCCATGCC AGCACCCAGA 
CAGATGAAGG AAAACCAGGT GCCCACCAAA GGAACATGGA GGAAAATCAA GGAAAGTGAC 
ATTTCTCCCA CAGGCATGGC TTCTCAGAGC GCTTCCTCAG GTGCTGCCAG TGGTGCTGAA 
TCCAAGCCTC TGATCTATCA GATGGCACCT CCTGTCTCTA AAACAGAGGA TGTTTGGGTG 
AGAATTGAGG ACTGCCCCAT TAACAACCCT AGATCTGGAC GGTCCCCCAC AGGCAACACC 
CCCCCAGTGA TTGACAGTGT TTCAGAGAAG GGAAGTTCAA GCATTAAAGA TTCAAAAGAC 
ACCCATGGGA AACAGAGTGT GGGCAGTGGC AGTCCTGTGC AAACCGTGGG TCTGGAAACC 
CGCCTCAACT CCTTTGTTCA GGTAGAGGCC CCAGAACAGA AAGGAACTGA GGCAAAACCA 
GGACAGAGTA ACCCAGTCTC TATAGCAGAG ACTGCTGAGA CGTGTATAGC AGAGCGTACC 
CCTTTCAGTT CCAGTAGCTC CAGCAAGCAC AGCTCACCTA GCGGGACTGT TGCTGCCAGA 
GTGACACCTT TTAATTACAA CCCTAGCCCT AGGAAGAGCA GCGCAGACAG CACTTCAGCC 
CGGCCGTCTC AGATCCCTAC GCCAGTGAGC ACCAACACGA AGAAGAGAGA TTCGAAGACT 
GACAGCACAG AATCCAGTGG AGCCCAAAGT CCTAAACGCC ATTCCGGGTC TTACCTCGTG 
ACGTCTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173293.1
CDS	294..8645
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_017317804.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenomatosis polyposis coli (Apc), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317804.1

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG GTGGAAATGG TGTATTCCTT GTTGTCAATG 
CTTGGTACTC ATGATAAGGA CGATATGTCA CGAACTTTGC TAGCTATGTC CAGCTCCCAA 
GACAGCTGTA TATCCATGCG GCAGTCTGGA TGTCTTCCTC TCCTCATCCA GCTTTTACAT 
GGCAATGACA AAGACTCTGT ATTGTTGGGA AATTCCCGGG GCAGTAAAGA GGCTCGGGCC 
AGGGCCAGTG CAGCACTCCA CAACATCATT CACTCACAGC CTGATGACAA GAGAGGCAGG 
CGTGAAATCC GAGTCCTTCA TCTTTTGGAA CAGATACGAG CTTACTGTGA AACCTGTTGG 
GAGTGGCAGG AAGCCCACGA ACAAGGCATG GACCAGGACA AAAACCCAAT GCCAGCTCCT 
GTTGAGCATC AGATCTGTCC TGCTGTGTGT GTTCTAATGA AGCTTTCATT TGATGAAGAG 
CATAGGCATG CAATGAATGA ACTTGGGGGA CTGCAGGCCA TTGCAGAGTT ATTGCAGGTG 
GACTGTGAGA TGTATGGGCT TACTAATGAC CACTACAGTG TTACTTTAAG ACGGTATGCT 
GGAATGGCTT TGACAAACTT GACCTTTGGA GATGTTGCCA ACAAGGCTAC GCTGTGTTCT 
ATGAAAGGCT GCATGAGAGC ACTTGTGGCC CAGTTAAAAT CTGAGAGTGA AGACTTACAG 
CAGGTTATTG CAAGTGTTTT GAGGAATTTG TCTTGGCGAG CAGATGTAAA TAGCAAAAAG 
ACGTTGAGAG AAGTTGGAAG TGTGAAAGCA TTGATGGAAT GTGCTTTGGA AGTTAAAAAG 
GAATCAACCC TCAAAAGCGT TTTGAGTGCC TTATGGAACC TGTCTGCACA CTGCACTGAG 
AATAAGGCTG ACATCTGTGC TGTGGATGGA GCACTGGCAT TTCTGGTTGG CACCCTCACT 
TACCGGAGCC AGACAAATAC TTTAGCCATT ATTGAAAGTG GAGGTGGGAT ATTACGGAAT 
GTGTCCAGCT TGATAGCTAC AAACGAAGAC CACAGGCAAA TCCTAAGAGA GAACAATTGC 
CTACAAACTT TATTACAGCA CTTGAAATCT CACAGCTTGA CAATAGTCAG TAATGCATGT 
GGAACTTTGT GGAATCTCTC AGCAAGAAAT CCTAAAGACC AGGAAGCCTT GTGGGACATG 
GGGGCAGTGA GCATGCTCAA GAACCTCATT CATTCCAAGC ACAAAATGAT TGCCATGGGA 
AGTGCAGCAG CTTTAAGGAA TCTCATGGCA AACAGACCTG CAAAGTATAA GGATGCCAAT 
ATCATGTCTC CCGGCTCAAG TCTGCCATCC CTTCACGTTA GGAAACAGAA AGCTCTAGAA 
GCTGAGCTAG ATGCTCAGCA TTTATCAGAA ACCTTCGACA ACATTGACAA CCTAAGTCCC 
AAGGCCTCTC ACCGGAGTAA GCAGAGACAC AAGCAGAATC TTTATGGTGA CTATGCTTTT 
GACGCCAATC GACATGATGA TAGTAGGTCA GACAATTTCA ATACTGGAAA CATGACTGTT 
CTTTCACCAT ATTTAAATAC TACGGTATTG CCCAGCTCTT CTTCCTCAAG GGGAAGTTTA 
GACAGTTCTC GTTCTGAGAA AGACAGAAGT TTGGAGAGAG AGCGAGGTAT TGGCCTCAGT 
GCTTACCATC CAACAACAGA AAATGCAGGA ACCTCATCAA AACGAGGTCT GCAGATCACT 
ACCACTGCAG CCCAGATAGC CAAAGTTATG GAAGAAGTAT CAGCCATTCA TACCTCCCAG 
GACGACAGAA GTTCTGCTTC TACCACCGAG TTCCATTGTG TGGCAGACGA CAGGAGTGCG 
GCACGAAGAA GCTCTGCCTC CCACACACAC TCAAACACAT ACAACTTCAC TAAGTCGGAA 
AATTCAAATA GGACATGCTC TATGCCTTAT GCCAAAGTGG AATATAAACG ATCTTCAAAT 
GACAGTTTAA ATAGTGTCAC TAGTAGTGAT GGATATGGTA AAAGAGGCCA AATGAAACCC 
TCAGTTGAAT CCTATTCTGA AGATGATGAA AGTAAATTTT GCAGTTATGG TCAGTATCCA 
GCTGACCTAG CCCATAAGAT ACACAGTGCA AATCATATGG ATGATAATGA TGGAGAACTG 
GATACACCAA TAAATTACAG TCTTAAATAT TCAGATGAGC AGTTGAACTC AGGAAGGCAG 
AGTCCCTCAC AGAATGAAAG GTGGGCAAGA CCAAAGCATG TGATAGAAGA TGAAATAAAG 
CAAAACGAGC AAAGACAAGC AAGAAGCCAG AACACCAGTT ATCCTGTCTA TTCTGAGAAT 
ACCGATGACA AACACCTCAA ATTCCAACCA CATTTTGGAC AACAAGAATG TGTTTCCCCA 
TATAGGTCAA GGGGAACCAG TGGTTCAGAA ACAAATCGAA TGGGTTCTAG TCATGCAATT 
AATCAAAATG TAAACCAGTC TCTGTGTCAG GAAGATGATT ATGAAGATGA TAAACCTACC 
AACTACAGTG AACGTTATTC TGAGGAAGAA CAACATGAAG AAGAAGAAGA GAGACCGACA 
AATTATAGCA TAAAATATAA TGAAGAGAAA CATCATGTGG ATCAGCCTAT TGATTATAGT 
TTAAAATATG CCACTGACAT TTCTTCCTCA CAAAAACCAT CATTTTCATT CTCAAAGAAT 
TCATCAGCAC AAAGCACTAA ACCTGAACAT CTCTCTCCAA GCAGCGAGAA TACAGCTGTA 
CCTCCATCTA ATGCCAAAAG GCAGAATCAG CTGCGTCCAA GTTCAGCACA AAGAAATGGC 
CAGACTCAAA AAGGCACTAC TTGCAAAGTC CCCTCCATCA ACCAAGAAAC AATACAGACT 
TACTGCGTAG AAGACACCCC AATATGTTTT TCAAGGTGCA GTTCATTATC ATCACTGTCA 
TCAGCTGACG ATGAAATAGG ATGTGATCAG ACAACACAGG AAGCAGATTC TGCTAATACT 
CTGCAGACAG CAGAAGTAAA AGAGAATGAT GTAACTCGGT CAGCTGAAGA TCCTGCAACT 
GAAGTTCCAG CAGTGTCCCA GAATGCTAGA GCCAAACCCA GCCGACTCCA GGCTTCTGGC 
TTATCTTCAG AATCAACCAG GCATAATAAA GCTGTTGAGT TTTCTTCAGG AGCCAAGTCT 
CCCTCCAAAA GTGGTGCTCA GACACCCAAA AGTCCCCCAG AACACTATGT CCAGGAGACT 
CCGCTCGTAT TCAGCAGGTG TACTTCTGTC AGCTCCCTTG ACAGTTTTGA GAGTCGCTCC 
ATTGCCAGCT CTGTTCAGAG TGAGCCATGT AGTGGAATGG TGAGTGGCAT CATAAGCCCC 
AGTGACCTTC CAGATAGTCC TGGGCAGACC ATGCCACCAA GCAGAAGCAA AACCCCTCCA 
CCTCCTCCAC AGACAGTGCA GGCCAAGAGA GAGGTGCCAA AAAGTAAAGT CCCTGCTGCT 
GAGAAGAGAG AGAGTGGGCC TAAGCAGACT GCTGTAAATG CTGCCGTGCA GAGGGTGCAG 
GTCCTTCCAG ACGTGGATAC TTTGTTACAC TTCGCCACAG AAAGTACTCC AGACGGGTTT 
TCTTGTTCCT CCAGCCTAAG TGCTCTGAGC CTGGATGAGC CATTTATACA GAAAGATGTA 
GAATTAAGAA TCATGCCTCC AGTTCAGGAA AACGACAATG GGAATGAAAC TGAATCAGAA 
CAGCCTGAGG AATCAAATGA AAACCAGGAT AAAGAGGTAG AAAAGCCTGA CTCTGAAAAA 
GACTTATTAG ATGATTCTGA TGACGATGAT ATTGAAATAT TAGAAGAATG TATTATTTCA 
GCCATGCCAA CAAAGTCATC ACGCAAAGCC AAAAAACTAG CCCAGACTGC TTCAAAATTA 
CCTCCACCTG TGGCAAGGAA ACCAAGTCAG CTACCTGTGT ATAAACTTCT GCCAGCACAG 
AATAGGCTGC AGGCACAAAA ACATGTTAGC TTTACACCAG GGGATGATGT GCCCCGGGTG 
TACTGTGTAG AAGGGACACC TATAAACTTT TCCACAGCAA CGTCTCTAAG TGATCTGACA 
ATAGAGTCCC CTCCAAATGA ATTGGCTACT GGAGATGGGG TCAGAGCGGG TATACAGTCA 
GGTGAATTTG AAAAACGAGA TACCATTCCT ACAGAAGGCA GAAGTACAGA TGATGCTCAG 
CGAGGAAAAA TCTCATCTAT AGTTACACCA GACCTGGATG ACAACAAAGC AGAGGAAGGA 
GATATTCTTG CAGAATGTAT CAATTCTGCT ATGCCCAAAG GAAAAAGCCA CAAGCCTTTC 
CGAGTGAAAA AGATAATGGA CCAAGTCCAA CAAGCATCCT CGACTTCATC TGGAGCTAAC 
AAAAATCAAG TAGACACTAA GAAAAAGAAG CCTACTTCAC CAGTAAAGCC CATGCCACAA 
AATACTGAAT ATAGAACGCG TGTGAGAAAG AATACAGACT CAAAAGTTAA TGTAAATACT 
GAAGAAACTT TCTCAGACAA CAAAGACTCA AAGAAACCAA GCTTACAAAC CAATGCCAAG 
GCCTTCAATG AAAAGCTACC TAACAATGAA GACAGAGTGC GGGGGAGCTT CGCCTTGGAC 
TCACCGCATC ACTACACCCC TATTGAGGGG ACGCCGTACT GCTTTTCCCG AAATGACTCC 
TTGAGTTCTC TGGATTTTGA TGATGACGAT GTTGACCTTT CCAGGGAAAA GGCCGAGTTA 
AGAAAGGGCA AAGAAAGCAA GGATTCCGAA GCCAAAGTTA CCTGCCGCCC AGAACCAAAC 
TCAAGCCAGC AGGCAGCTAG TAAGTCACAA GCCAGTATAA AACATCCAGC AAACAGAGCA 
CAGTCCAAAC CAGTGCTGCA GAAACAGCCC ACTTTCCCCC AGTCCTCCAA AGACGGACCA 
GATAGAGGGG CAGCAACTGA CGAAAAACTG CAGAATTTTG CTATTGAAAA TACTCCAGTT 
TGCTTTTCTC GAAATTCCTC TCTGAGTTCC CTTAGTGACA TTGACCAGGA AAACAACAAT 
AACAAAGAAA GTGAACCAAT CAAAGAAGCT GAACCTGCCA ACTCACAAGG AGAGCCCAGT 
AAGCCTCAGG CATCCGGGTA TGCTCCCAAG TCCTTCCACG TCGAAGACAC CCCTGTCTGT 
TTCTCAAGAA ACAGCTCTCT CAGTTCTCTT AGCATTGACT CTGAGGACGA CCTGTTACAG 
GAGTGTATAA GTTCTGCCAT GCCAAAAAAG AAAAGGCCTT CAAGACTCAA GAGTGAGAGC 
GAAAAGCAGA GCCCTAGAAA AGTGGGTGGC ATATTAGCTG AAGACCTGAC GCTTGATTTG 
AAAGATCTAC AGAGGCCAGA TTCAGAACAC GCTTTCTCCC CCGACTCAGA AAATTTTGAC 
TGGAAAGCTA TTCAGGAAGG CGCAAACTCC ATAGTAAGTA GTTTGCACCA AGCTGCTGCA 
GCCGCCGCGT GCTTATCTAG ACAAGCGTCA TCCGACTCAG ATTCCATTCT GTCACTAAAG 
TCCGGCATTT CTCTGGGATC GCCTTTTCAT CTTACACCTG ATCAAGAGGA AAAGCCATTC 
ACAAGCAATA AAGGCCCAAG AATTCTCAAA CCTGGAGAGA AAAGCACATT AGAAGCAAAA 
AAAATAGAAT CTGAAAACAA AGGAATCAAA GGCGGGAAAA AGGTTTATAA AAGCTTGATT 
ACGGGAAAGA TTCGCTCCAA TTCAGAAATT TCCAGCCAAA TGAAACAACC CCTCCCGACA 
AACATGCCTT CAATCTCAAG AGGCAGGACG ATGATTCACA TCCCAGGGCT TCGGAATAGC 
TCCTCTAGTA CAAGCCCTGT CTCTAAGAAA GGCCCACCCC TCAAGACTCC AGCCTCTAAA 
AGCCCCAGTG AAGGGCCGGG AGCTACCACT TCTCCTCGAG GAACTAAGCC AGCAGGAAAG 
TCAGAGCTTA GCCCTATCAC CAGGCAAACT TCCCAAATCA GTGGGTCAAA TAAGGGGTCT 
TCTAGATCAG GATCTAGAGA CTCCACTCCC TCAAGACCTA CACAGCAACC ATTAAGTAGG 
CCAATGCAGT CTCCAGGGCG AAACTCAATT TCCCCTGGTA GAAATGGAAT AAGCCCTCCT 
AACAAACTGT CTCAGCTGCC CAGAACATCA TCTCCCAGTA CTGCTTCAAC TAAGTCCTCC 
GGTTCTGGGA AAATGTCATA TACATCCCCA GGTAGACAGC TGAGCCAACA AAATCTTACC 
AAACAAGCAA GTTTATCCAA GAATGCCAGC AGTATCCCCA GAAGTGAGTC GGCATCTAAA 
GGACTGAATC AGATGAGTAA CGGCAATGGG TCAAATAAAA AGGTAGAACT TTCTAGAATG 
TCTTCAACTA AATCAAGTGG AAGTGAATCA GACAGATCAG AAAGGCCTGC ATTAGTACGC 
CAGTCTACTT TCATCAAAGA AGCCCCAAGC CCAACCCTGA GGAGGAAACT GGAGGAATCT 
GCCTCATTTG AATCCCTTTC TCCATCTTCT AGACCAGATT CTCCCACCAG GTCGCAGGCA 
CAGACCCCAG TTTTAAGCCC TTCCCTTCCT GATATGTCTC TGTCCACACA TCCATCTGTT 
CAGGCAGGTG GGTGGCGAAA GCTCCCGCCT AATCTCAGCC CCACTATCGA GTATAATGAC 
GGAAGGCCCA CAAAACGGCA TGATATTGCA CGCTCCCATT CTGAAAGTCC TTCCAGACTA 
CCAATCAACC GGGCGGGAAC CTGGAAGCGT GAACACAGCA AACATTCCTC GTCCCTTCCT 
CGAGTGAGTA CTTGGAGAAG AACTGGAAGC TCATCTTCTA TTCTTTCTGC TTCATCAGAG 
TCCAGTGAAA AAGCAAAAAG TGAGGATGAA AGGCATGTGA GCTCCATGCC AGCACCCAGA 
CAGATGAAGG AAAACCAGGT GCCCACCAAA GGAACATGGA GGAAAATCAA GGAAAGTGAC 
ATTTCTCCCA CAGGCATGGC TTCTCAGAGC GCTTCCTCAG GTGCTGCCAG TGGTGCTGAA 
TCCAAGCCTC TGATCTATCA GATGGCACCT CCTGTCTCTA AAACAGAGGA TGTTTGGGTG 
AGAATTGAGG ACTGCCCCAT TAACAACCCT AGATCTGGAC GGTCCCCCAC AGGCAACACC 
CCCCCAGTGA TTGACAGTGT TTCAGAGAAG GGAAGTTCAA GCATTAAAGA TTCAAAAGAC 
ACCCATGGGA AACAGAGTGT GGGCAGTGGC AGTCCTGTGC AAACCGTGGG TCTGGAAACC 
CGCCTCAACT CCTTTGTTCA GGTAGAGGCC CCAGAACAGA AAGGAACTGA GGCAAAACCA 
GGACAGAGTA ACCCAGTCTC TATAGCAGAG ACTGCTGAGA CGTGTATAGC AGAGCGTACC 
CCTTTCAGTT CCAGTAGCTC CAGCAAGCAC AGCTCACCTA GCGGGACTGT TGCTGCCAGA 
GTGACACCTT TTAATTACAA CCCTAGCCCT AGGAAGAGCA GCGCAGACAG CACTTCAGCC 
CGGCCGTCTC AGATCCCTAC GCCAGTGAGC ACCAACACGA AGAAGAGAGA TTCGAAGACT 
GACAGCACAG AATCCAGTGG AGCCCAAAGT CCTAAACGCC ATTCCGGGTC TTACCTCGTG 
ACGTCTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250278.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106138.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X7, mRNA.

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CloneID	OMu87321
Clone ID Related Accession (Same CDS sequence)	XM_030250278.1
Accession Version	XM_030250278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGTCCAG CTACACGTGT GGATCACGAA ACAGCCAGTG TTTTGAGTTC TAGCGGCACG 
CACTCTGCTC CTCGAAGGTT GACAAGTCAT CTGGGGACAA AGGTGGAAAT GGTGTATTCC 
TTGTTGTCAA TGCTTGGTAC TCATGATAAG GACGATATGT CACGAACTTT GCTAGCTATG 
TCCAGCTCCC AAGACAGCTG TATATCCATG CGGCAGTCTG GATGTCTTCC TCTCCTCATC 
CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTATTGTTGG GAAATTCCCG GGGCAGTAAA 
GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC 
AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT 
GAAACCTGTT GGGAGTGGCA GGAAGCCCAC GAACAAGGCA TGGACCAGGA CAAAAACCCA 
ATGCCAGCTC CTGTTGAGCA TCAGATCTGT CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA 
TTTGATGAAG AGCATAGGCA TGCAATGAAT GAACTTGGTA GGAAGGCTAC CCGGGGCATT 
TCATCCCAGG AGCTAGGTCA GGGGCTTTCA GGGGGACTGC AGGCCATTGC AGAGTTATTG 
CAGGTGGACT GTGAGATGTA TGGGCTTACT AATGACCACT ACAGTGTTAC TTTAAGACGG 
TATGCTGGAA TGGCTTTGAC AAACTTGACC TTTGGAGATG TTGCCAACAA GGCTACGCTG 
TGTTCTATGA AAGGCTGCAT GAGAGCACTT GTGGCCCAGT TAAAATCTGA GAGTGAAGAC 
TTACAGCAGG TTATTGCAAG TGTTTTGAGG AATTTGTCTT GGCGAGCAGA TGTAAATAGC 
AAAAAGACGT TGAGAGAAGT TGGAAGTGTG AAAGCATTGA TGGAATGTGC TTTGGAAGTT 
AAAAAGGAAT CAACCCTCAA AAGCGTTTTG AGTGCCTTAT GGAACCTGTC TGCACACTGC 
ACTGAGAATA AGGCTGACAT CTGTGCTGTG GATGGAGCAC TGGCATTTCT GGTTGGCACC 
CTCACTTACC GGAGCCAGAC AAATACTTTA GCCATTATTG AAAGTGGAGG TGGGATATTA 
CGGAATGTGT CCAGCTTGAT AGCTACAAAC GAAGACCACA GGCAAATCCT AAGAGAGAAC 
AATTGCCTAC AAACTTTATT ACAGCACTTG AAATCTCACA GCTTGACAAT AGTCAGTAAT 
GCATGTGGAA CTTTGTGGAA TCTCTCAGCA AGAAATCCTA AAGACCAGGA AGCCTTGTGG 
GACATGGGGG CAGTGAGCAT GCTCAAGAAC CTCATTCATT CCAAGCACAA AATGATTGCC 
ATGGGAAGTG CAGCAGCTTT AAGGAATCTC ATGGCAAACA GACCTGCAAA GTATAAGGAT 
GCCAATATCA TGTCTCCCGG CTCAAGTCTG CCATCCCTTC ACGTTAGGAA ACAGAAAGCT 
CTAGAAGCTG AGCTAGATGC TCAGCATTTA TCAGAAACCT TCGACAACAT TGACAACCTA 
AGTCCCAAGG CCTCTCACCG GAGTAAGCAG AGACACAAGC AGAATCTTTA TGGTGACTAT 
GCTTTTGACG CCAATCGACA TGATGATAGT AGGTCAGACA ATTTCAATAC TGGAAACATG 
ACTGTTCTTT CACCATATTT AAATACTACG GTATTGCCCA GCTCTTCTTC CTCAAGGGGA 
AGTTTAGACA GTTCTCGTTC TGAGAAAGAC AGAAGTTTGG AGAGAGAGCG AGGTATTGGC 
CTCAGTGCTT ACCATCCAAC AACAGAAAAT GCAGGAACCT CATCAAAACG AGGTCTGCAG 
ATCACTACCA CTGCAGCCCA GATAGCCAAA GTTATGGAAG AAGTATCAGC CATTCATACC 
TCCCAGGACG ACAGAAGTTC TGCTTCTACC ACCGAGTTCC ATTGTGTGGC AGACGACAGG 
AGTGCGGCAC GAAGAAGCTC TGCCTCCCAC ACACACTCAA ACACATACAA CTTCACTAAG 
TCGGAAAATT CAAATAGGAC ATGCTCTATG CCTTATGCCA AAGTGGAATA TAAACGATCT 
TCAAATGACA GTTTAAATAG TGTCACTAGT AGTGATGGAT ATGGTAAAAG AGGCCAAATG 
AAACCCTCAG TTGAATCCTA TTCTGAAGAT GATGAAAGTA AATTTTGCAG TTATGGTCAG 
TATCCAGCTG ACCTAGCCCA TAAGATACAC AGTGCAAATC ATATGGATGA TAATGATGGA 
GAACTGGATA CACCAATAAA TTACAGTCTT AAATATTCAG ATGAGCAGTT GAACTCAGGA 
AGGCAGAGTC CCTCACAGAA TGAAAGGTGG GCAAGACCAA AGCATGTGAT AGAAGATGAA 
ATAAAGCAAA ACGAGCAAAG ACAAGCAAGA AGCCAGAACA CCAGTTATCC TGTCTATTCT 
GAGAATACCG ATGACAAACA CCTCAAATTC CAACCACATT TTGGACAACA AGAATGTGTT 
TCCCCATATA GGTCAAGGGG AACCAGTGGT TCAGAAACAA ATCGAATGGG TTCTAGTCAT 
GCAATTAATC AAAATGTAAA CCAGTCTCTG TGTCAGGAAG ATGATTATGA AGATGATAAA 
CCTACCAACT ACAGTGAACG TTATTCTGAG GAAGAACAAC ATGAAGAAGA AGAAGAGAGA 
CCGACAAATT ATAGCATAAA ATATAATGAA GAGAAACATC ATGTGGATCA GCCTATTGAT 
TATAGTTTAA AATATGCCAC TGACATTTCT TCCTCACAAA AACCATCATT TTCATTCTCA 
AAGAATTCAT CAGCACAAAG CACTAAACCT GAACATCTCT CTCCAAGCAG CGAGAATACA 
GCTGTACCTC CATCTAATGC CAAAAGGCAG AATCAGCTGC GTCCAAGTTC AGCACAAAGA 
AATGGCCAGA CTCAAAAAGG CACTACTTGC AAAGTCCCCT CCATCAACCA AGAAACAATA 
CAGACTTACT GCGTAGAAGA CACCCCAATA TGTTTTTCAA GGTGCAGTTC ATTATCATCA 
CTGTCATCAG CTGACGATGA AATAGGATGT GATCAGACAA CACAGGAAGC AGATTCTGCT 
AATACTCTGC AGACAGCAGA AGTAAAAGAG AATGATGTAA CTCGGTCAGC TGAAGATCCT 
GCAACTGAAG TTCCAGCAGT GTCCCAGAAT GCTAGAGCCA AACCCAGCCG ACTCCAGGCT 
TCTGGCTTAT CTTCAGAATC AACCAGGCAT AATAAAGCTG TTGAGTTTTC TTCAGGAGCC 
AAGTCTCCCT CCAAAAGTGG TGCTCAGACA CCCAAAAGTC CCCCAGAACA CTATGTCCAG 
GAGACTCCGC TCGTATTCAG CAGGTGTACT TCTGTCAGCT CCCTTGACAG TTTTGAGAGT 
CGCTCCATTG CCAGCTCTGT TCAGAGTGAG CCATGTAGTG GAATGGTGAG TGGCATCATA 
AGCCCCAGTG ACCTTCCAGA TAGTCCTGGG CAGACCATGC CACCAAGCAG AAGCAAAACC 
CCTCCACCTC CTCCACAGAC AGTGCAGGCC AAGAGAGAGG TGCCAAAAAG TAAAGTCCCT 
GCTGCTGAGA AGAGAGAGAG TGGGCCTAAG CAGACTGCTG TAAATGCTGC CGTGCAGAGG 
GTGCAGGTCC TTCCAGACGT GGATACTTTG TTACACTTCG CCACAGAAAG TACTCCAGAC 
GGGTTTTCTT GTTCCTCCAG CCTAAGTGCT CTGAGCCTGG ATGAGCCATT TATACAGAAA 
GATGTAGAAT TAAGAATCAT GCCTCCAGTT CAGGAAAACG ACAATGGGAA TGAAACTGAA 
TCAGAACAGC CTGAGGAATC AAATGAAAAC CAGGATAAAG AGGTAGAAAA GCCTGACTCT 
GAAAAAGACT TATTAGATGA TTCTGATGAC GATGATATTG AAATATTAGA AGAATGTATT 
ATTTCAGCCA TGCCAACAAA GTCATCACGC AAAGCCAAAA AACTAGCCCA GACTGCTTCA 
AAATTACCTC CACCTGTGGC AAGGAAACCA AGTCAGCTAC CTGTGTATAA ACTTCTGCCA 
GCACAGAATA GGCTGCAGGC ACAAAAACAT GTTAGCTTTA CACCAGGGGA TGATGTGCCC 
CGGGTGTACT GTGTAGAAGG GACACCTATA AACTTTTCCA CAGCAACGTC TCTAAGTGAT 
CTGACAATAG AGTCCCCTCC AAATGAATTG GCTACTGGAG ATGGGGTCAG AGCGGGTATA 
CAGTCAGGTG AATTTGAAAA ACGAGATACC ATTCCTACAG AAGGCAGAAG TACAGATGAT 
GCTCAGCGAG GAAAAATCTC ATCTATAGTT ACACCAGACC TGGATGACAA CAAAGCAGAG 
GAAGGAGATA TTCTTGCAGA ATGTATCAAT TCTGCTATGC CCAAAGGAAA AAGCCACAAG 
CCTTTCCGAG TGAAAAAGAT AATGGACCAA GTCCAACAAG CATCCTCGAC TTCATCTGGA 
GCTAACAAAA ATCAAGTAGA CACTAAGAAA AAGAAGCCTA CTTCACCAGT AAAGCCCATG 
CCACAAAATA CTGAATATAG AACGCGTGTG AGAAAGAATA CAGACTCAAA AGTTAATGTA 
AATACTGAAG AAACTTTCTC AGACAACAAA GACTCAAAGA AACCAAGCTT ACAAACCAAT 
GCCAAGGCCT TCAATGAAAA GCTACCTAAC AATGAAGACA GAGTGCGGGG GAGCTTCGCC 
TTGGACTCAC CGCATCACTA CACCCCTATT GAGGGGACGC CGTACTGCTT TTCCCGAAAT 
GACTCCTTGA GTTCTCTGGA TTTTGATGAT GACGATGTTG ACCTTTCCAG GGAAAAGGCC 
GAGTTAAGAA AGGGCAAAGA AAGCAAGGAT TCCGAAGCCA AAGTTACCTG CCGCCCAGAA 
CCAAACTCAA GCCAGCAGGC AGCTAGTAAG TCACAAGCCA GTATAAAACA TCCAGCAAAC 
AGAGCACAGT CCAAACCAGT GCTGCAGAAA CAGCCCACTT TCCCCCAGTC CTCCAAAGAC 
GGACCAGATA GAGGGGCAGC AACTGACGAA AAACTGCAGA ATTTTGCTAT TGAAAATACT 
CCAGTTTGCT TTTCTCGAAA TTCCTCTCTG AGTTCCCTTA GTGACATTGA CCAGGAAAAC 
AACAATAACA AAGAAAGTGA ACCAATCAAA GAAGCTGAAC CTGCCAACTC ACAAGGAGAG 
CCCAGTAAGC CTCAGGCATC CGGGTATGCT CCCAAGTCCT TCCACGTCGA AGACACCCCT 
GTCTGTTTCT CAAGAAACAG CTCTCTCAGT TCTCTTAGCA TTGACTCTGA GGACGACCTG 
TTACAGGAGT GTATAAGTTC TGCCATGCCA AAAAAGAAAA GGCCTTCAAG ACTCAAGAGT 
GAGAGCGAAA AGCAGAGCCC TAGAAAAGTG GGTGGCATAT TAGCTGAAGA CCTGACGCTT 
GATTTGAAAG ATCTACAGAG GCCAGATTCA GAACACGCTT TCTCCCCCGA CTCAGAAAAT 
TTTGACTGGA AAGCTATTCA GGAAGGCGCA AACTCCATAG TAAGTAGTTT GCACCAAGCT 
GCTGCAGCCG CCGCGTGCTT ATCTAGACAA GCGTCATCCG ACTCAGATTC CATTCTGTCA 
CTAAAGTCCG GCATTTCTCT GGGATCGCCT TTTCATCTTA CACCTGATCA AGAGGAAAAG 
CCATTCACAA GCAATAAAGG CCCAAGAATT CTCAAACCTG GAGAGAAAAG CACATTAGAA 
GCAAAAAAAA TAGAATCTGA AAACAAAGGA ATCAAAGGCG GGAAAAAGGT TTATAAAAGC 
TTGATTACGG GAAAGATTCG CTCCAATTCA GAAATTTCCA GCCAAATGAA ACAACCCCTC 
CCGACAAACA TGCCTTCAAT CTCAAGAGGC AGGACGATGA TTCACATCCC AGGGCTTCGG 
AATAGCTCCT CTAGTACAAG CCCTGTCTCT AAGAAAGGCC CACCCCTCAA GACTCCAGCC 
TCTAAAAGCC CCAGTGAAGG GCCGGGAGCT ACCACTTCTC CTCGAGGAAC TAAGCCAGCA 
GGAAAGTCAG AGCTTAGCCC TATCACCAGG CAAACTTCCC AAATCAGTGG GTCAAATAAG 
GGGTCTTCTA GATCAGGATC TAGAGACTCC ACTCCCTCAA GACCTACACA GCAACCATTA 
AGTAGGCCAA TGCAGTCTCC AGGGCGAAAC TCAATTTCCC CTGGTAGAAA TGGAATAAGC 
CCTCCTAACA AACTGTCTCA GCTGCCCAGA ACATCATCTC CCAGTACTGC TTCAACTAAG 
TCCTCCGGTT CTGGGAAAAT GTCATATACA TCCCCAGGTA GACAGCTGAG CCAACAAAAT 
CTTACCAAAC AAGCAAGTTT ATCCAAGAAT GCCAGCAGTA TCCCCAGAAG TGAGTCGGCA 
TCTAAAGGAC TGAATCAGAT GAGTAACGGC AATGGGTCAA ATAAAAAGGT AGAACTTTCT 
AGAATGTCTT CAACTAAATC AAGTGGAAGT GAATCAGACA GATCAGAAAG GCCTGCATTA 
GTACGCCAGT CTACTTTCAT CAAAGAAGCC CCAAGCCCAA CCCTGAGGAG GAAACTGGAG 
GAATCTGCCT CATTTGAATC CCTTTCTCCA TCTTCTAGAC CAGATTCTCC CACCAGGTCG 
CAGGCACAGA CCCCAGTTTT AAGCCCTTCC CTTCCTGATA TGTCTCTGTC CACACATCCA 
TCTGTTCAGG CAGGTGGGTG GCGAAAGCTC CCGCCTAATC TCAGCCCCAC TATCGAGTAT 
AATGACGGAA GGCCCACAAA ACGGCATGAT ATTGCACGCT CCCATTCTGA AAGTCCTTCC 
AGACTACCAA TCAACCGGGC GGGAACCTGG AAGCGTGAAC ACAGCAAACA TTCCTCGTCC 
CTTCCTCGAG TGAGTACTTG GAGAAGAACT GGAAGCTCAT CTTCTATTCT TTCTGCTTCA 
TCAGAGTCCA GTGAAAAAGC AAAAAGTGAG GATGAAAGGC ATGTGAGCTC CATGCCAGCA 
CCCAGACAGA TGAAGGAAAA CCAGGTGCCC ACCAAAGGAA CATGGAGGAA AATCAAGGAA 
AGTGACATTT CTCCCACAGG CATGGCTTCT CAGAGCGCTT CCTCAGGTGC TGCCAGTGGT 
GCTGAATCCA AGCCTCTGAT CTATCAGATG GCACCTCCTG TCTCTAAAAC AGAGGATGTT 
TGGGTGAGAA TTGAGGACTG CCCCATTAAC AACCCTAGAT CTGGACGGTC CCCCACAGGC 
AACACCCCCC CAGTGATTGA CAGTGTTTCA GAGAAGGGAA GTTCAAGCAT TAAAGATTCA 
AAAGACACCC ATGGGAAACA GAGTGTGGGC AGTGGCAGTC CTGTGCAAAC CGTGGGTCTG 
GAAACCCGCC TCAACTCCTT TGTTCAGGTA GAGGCCCCAG AACAGAAAGG AACTGAGGCA 
AAACCAGGAC AGAGTAACCC AGTCTCTATA GCAGAGACTG CTGAGACGTG TATAGCAGAG 
CGTACCCCTT TCAGTTCCAG TAGCTCCAGC AAGCACAGCT CACCTAGCGG GACTGTTGCT 
GCCAGAGTGA CACCTTTTAA TTACAACCCT AGCCCTAGGA AGAGCAGCGC AGACAGCACT 
TCAGCCCGGC CGTCTCAGAT CCCTACGCCA GTGAGCACCA ACACGAAGAA GAGAGATTCG 
AAGACTGACA GCACAGAATC CAGTGGAGCC CAAAGTCCTA AACGCCATTC CGGGTCTTAC 
CTCGTGACGT CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106138.1
CDS	101..8578
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAESPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250278.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250278.1

ORF Insert Sequence:

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGTCCAG CTACACGTGT GGATCACGAA ACAGCCAGTG TTTTGAGTTC TAGCGGCACG 
CACTCTGCTC CTCGAAGGTT GACAAGTCAT CTGGGGACAA AGGTGGAAAT GGTGTATTCC 
TTGTTGTCAA TGCTTGGTAC TCATGATAAG GACGATATGT CACGAACTTT GCTAGCTATG 
TCCAGCTCCC AAGACAGCTG TATATCCATG CGGCAGTCTG GATGTCTTCC TCTCCTCATC 
CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTATTGTTGG GAAATTCCCG GGGCAGTAAA 
GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC 
AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT 
GAAACCTGTT GGGAGTGGCA GGAAGCCCAC GAACAAGGCA TGGACCAGGA CAAAAACCCA 
ATGCCAGCTC CTGTTGAGCA TCAGATCTGT CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA 
TTTGATGAAG AGCATAGGCA TGCAATGAAT GAACTTGGTA GGAAGGCTAC CCGGGGCATT 
TCATCCCAGG AGCTAGGTCA GGGGCTTTCA GGGGGACTGC AGGCCATTGC AGAGTTATTG 
CAGGTGGACT GTGAGATGTA TGGGCTTACT AATGACCACT ACAGTGTTAC TTTAAGACGG 
TATGCTGGAA TGGCTTTGAC AAACTTGACC TTTGGAGATG TTGCCAACAA GGCTACGCTG 
TGTTCTATGA AAGGCTGCAT GAGAGCACTT GTGGCCCAGT TAAAATCTGA GAGTGAAGAC 
TTACAGCAGG TTATTGCAAG TGTTTTGAGG AATTTGTCTT GGCGAGCAGA TGTAAATAGC 
AAAAAGACGT TGAGAGAAGT TGGAAGTGTG AAAGCATTGA TGGAATGTGC TTTGGAAGTT 
AAAAAGGAAT CAACCCTCAA AAGCGTTTTG AGTGCCTTAT GGAACCTGTC TGCACACTGC 
ACTGAGAATA AGGCTGACAT CTGTGCTGTG GATGGAGCAC TGGCATTTCT GGTTGGCACC 
CTCACTTACC GGAGCCAGAC AAATACTTTA GCCATTATTG AAAGTGGAGG TGGGATATTA 
CGGAATGTGT CCAGCTTGAT AGCTACAAAC GAAGACCACA GGCAAATCCT AAGAGAGAAC 
AATTGCCTAC AAACTTTATT ACAGCACTTG AAATCTCACA GCTTGACAAT AGTCAGTAAT 
GCATGTGGAA CTTTGTGGAA TCTCTCAGCA AGAAATCCTA AAGACCAGGA AGCCTTGTGG 
GACATGGGGG CAGTGAGCAT GCTCAAGAAC CTCATTCATT CCAAGCACAA AATGATTGCC 
ATGGGAAGTG CAGCAGCTTT AAGGAATCTC ATGGCAAACA GACCTGCAAA GTATAAGGAT 
GCCAATATCA TGTCTCCCGG CTCAAGTCTG CCATCCCTTC ACGTTAGGAA ACAGAAAGCT 
CTAGAAGCTG AGCTAGATGC TCAGCATTTA TCAGAAACCT TCGACAACAT TGACAACCTA 
AGTCCCAAGG CCTCTCACCG GAGTAAGCAG AGACACAAGC AGAATCTTTA TGGTGACTAT 
GCTTTTGACG CCAATCGACA TGATGATAGT AGGTCAGACA ATTTCAATAC TGGAAACATG 
ACTGTTCTTT CACCATATTT AAATACTACG GTATTGCCCA GCTCTTCTTC CTCAAGGGGA 
AGTTTAGACA GTTCTCGTTC TGAGAAAGAC AGAAGTTTGG AGAGAGAGCG AGGTATTGGC 
CTCAGTGCTT ACCATCCAAC AACAGAAAAT GCAGGAACCT CATCAAAACG AGGTCTGCAG 
ATCACTACCA CTGCAGCCCA GATAGCCAAA GTTATGGAAG AAGTATCAGC CATTCATACC 
TCCCAGGACG ACAGAAGTTC TGCTTCTACC ACCGAGTTCC ATTGTGTGGC AGACGACAGG 
AGTGCGGCAC GAAGAAGCTC TGCCTCCCAC ACACACTCAA ACACATACAA CTTCACTAAG 
TCGGAAAATT CAAATAGGAC ATGCTCTATG CCTTATGCCA AAGTGGAATA TAAACGATCT 
TCAAATGACA GTTTAAATAG TGTCACTAGT AGTGATGGAT ATGGTAAAAG AGGCCAAATG 
AAACCCTCAG TTGAATCCTA TTCTGAAGAT GATGAAAGTA AATTTTGCAG TTATGGTCAG 
TATCCAGCTG ACCTAGCCCA TAAGATACAC AGTGCAAATC ATATGGATGA TAATGATGGA 
GAACTGGATA CACCAATAAA TTACAGTCTT AAATATTCAG ATGAGCAGTT GAACTCAGGA 
AGGCAGAGTC CCTCACAGAA TGAAAGGTGG GCAAGACCAA AGCATGTGAT AGAAGATGAA 
ATAAAGCAAA ACGAGCAAAG ACAAGCAAGA AGCCAGAACA CCAGTTATCC TGTCTATTCT 
GAGAATACCG ATGACAAACA CCTCAAATTC CAACCACATT TTGGACAACA AGAATGTGTT 
TCCCCATATA GGTCAAGGGG AACCAGTGGT TCAGAAACAA ATCGAATGGG TTCTAGTCAT 
GCAATTAATC AAAATGTAAA CCAGTCTCTG TGTCAGGAAG ATGATTATGA AGATGATAAA 
CCTACCAACT ACAGTGAACG TTATTCTGAG GAAGAACAAC ATGAAGAAGA AGAAGAGAGA 
CCGACAAATT ATAGCATAAA ATATAATGAA GAGAAACATC ATGTGGATCA GCCTATTGAT 
TATAGTTTAA AATATGCCAC TGACATTTCT TCCTCACAAA AACCATCATT TTCATTCTCA 
AAGAATTCAT CAGCACAAAG CACTAAACCT GAACATCTCT CTCCAAGCAG CGAGAATACA 
GCTGTACCTC CATCTAATGC CAAAAGGCAG AATCAGCTGC GTCCAAGTTC AGCACAAAGA 
AATGGCCAGA CTCAAAAAGG CACTACTTGC AAAGTCCCCT CCATCAACCA AGAAACAATA 
CAGACTTACT GCGTAGAAGA CACCCCAATA TGTTTTTCAA GGTGCAGTTC ATTATCATCA 
CTGTCATCAG CTGACGATGA AATAGGATGT GATCAGACAA CACAGGAAGC AGATTCTGCT 
AATACTCTGC AGACAGCAGA AGTAAAAGAG AATGATGTAA CTCGGTCAGC TGAAGATCCT 
GCAACTGAAG TTCCAGCAGT GTCCCAGAAT GCTAGAGCCA AACCCAGCCG ACTCCAGGCT 
TCTGGCTTAT CTTCAGAATC AACCAGGCAT AATAAAGCTG TTGAGTTTTC TTCAGGAGCC 
AAGTCTCCCT CCAAAAGTGG TGCTCAGACA CCCAAAAGTC CCCCAGAACA CTATGTCCAG 
GAGACTCCGC TCGTATTCAG CAGGTGTACT TCTGTCAGCT CCCTTGACAG TTTTGAGAGT 
CGCTCCATTG CCAGCTCTGT TCAGAGTGAG CCATGTAGTG GAATGGTGAG TGGCATCATA 
AGCCCCAGTG ACCTTCCAGA TAGTCCTGGG CAGACCATGC CACCAAGCAG AAGCAAAACC 
CCTCCACCTC CTCCACAGAC AGTGCAGGCC AAGAGAGAGG TGCCAAAAAG TAAAGTCCCT 
GCTGCTGAGA AGAGAGAGAG TGGGCCTAAG CAGACTGCTG TAAATGCTGC CGTGCAGAGG 
GTGCAGGTCC TTCCAGACGT GGATACTTTG TTACACTTCG CCACAGAAAG TACTCCAGAC 
GGGTTTTCTT GTTCCTCCAG CCTAAGTGCT CTGAGCCTGG ATGAGCCATT TATACAGAAA 
GATGTAGAAT TAAGAATCAT GCCTCCAGTT CAGGAAAACG ACAATGGGAA TGAAACTGAA 
TCAGAACAGC CTGAGGAATC AAATGAAAAC CAGGATAAAG AGGTAGAAAA GCCTGACTCT 
GAAAAAGACT TATTAGATGA TTCTGATGAC GATGATATTG AAATATTAGA AGAATGTATT 
ATTTCAGCCA TGCCAACAAA GTCATCACGC AAAGCCAAAA AACTAGCCCA GACTGCTTCA 
AAATTACCTC CACCTGTGGC AAGGAAACCA AGTCAGCTAC CTGTGTATAA ACTTCTGCCA 
GCACAGAATA GGCTGCAGGC ACAAAAACAT GTTAGCTTTA CACCAGGGGA TGATGTGCCC 
CGGGTGTACT GTGTAGAAGG GACACCTATA AACTTTTCCA CAGCAACGTC TCTAAGTGAT 
CTGACAATAG AGTCCCCTCC AAATGAATTG GCTACTGGAG ATGGGGTCAG AGCGGGTATA 
CAGTCAGGTG AATTTGAAAA ACGAGATACC ATTCCTACAG AAGGCAGAAG TACAGATGAT 
GCTCAGCGAG GAAAAATCTC ATCTATAGTT ACACCAGACC TGGATGACAA CAAAGCAGAG 
GAAGGAGATA TTCTTGCAGA ATGTATCAAT TCTGCTATGC CCAAAGGAAA AAGCCACAAG 
CCTTTCCGAG TGAAAAAGAT AATGGACCAA GTCCAACAAG CATCCTCGAC TTCATCTGGA 
GCTAACAAAA ATCAAGTAGA CACTAAGAAA AAGAAGCCTA CTTCACCAGT AAAGCCCATG 
CCACAAAATA CTGAATATAG AACGCGTGTG AGAAAGAATA CAGACTCAAA AGTTAATGTA 
AATACTGAAG AAACTTTCTC AGACAACAAA GACTCAAAGA AACCAAGCTT ACAAACCAAT 
GCCAAGGCCT TCAATGAAAA GCTACCTAAC AATGAAGACA GAGTGCGGGG GAGCTTCGCC 
TTGGACTCAC CGCATCACTA CACCCCTATT GAGGGGACGC CGTACTGCTT TTCCCGAAAT 
GACTCCTTGA GTTCTCTGGA TTTTGATGAT GACGATGTTG ACCTTTCCAG GGAAAAGGCC 
GAGTTAAGAA AGGGCAAAGA AAGCAAGGAT TCCGAAGCCA AAGTTACCTG CCGCCCAGAA 
CCAAACTCAA GCCAGCAGGC AGCTAGTAAG TCACAAGCCA GTATAAAACA TCCAGCAAAC 
AGAGCACAGT CCAAACCAGT GCTGCAGAAA CAGCCCACTT TCCCCCAGTC CTCCAAAGAC 
GGACCAGATA GAGGGGCAGC AACTGACGAA AAACTGCAGA ATTTTGCTAT TGAAAATACT 
CCAGTTTGCT TTTCTCGAAA TTCCTCTCTG AGTTCCCTTA GTGACATTGA CCAGGAAAAC 
AACAATAACA AAGAAAGTGA ACCAATCAAA GAAGCTGAAC CTGCCAACTC ACAAGGAGAG 
CCCAGTAAGC CTCAGGCATC CGGGTATGCT CCCAAGTCCT TCCACGTCGA AGACACCCCT 
GTCTGTTTCT CAAGAAACAG CTCTCTCAGT TCTCTTAGCA TTGACTCTGA GGACGACCTG 
TTACAGGAGT GTATAAGTTC TGCCATGCCA AAAAAGAAAA GGCCTTCAAG ACTCAAGAGT 
GAGAGCGAAA AGCAGAGCCC TAGAAAAGTG GGTGGCATAT TAGCTGAAGA CCTGACGCTT 
GATTTGAAAG ATCTACAGAG GCCAGATTCA GAACACGCTT TCTCCCCCGA CTCAGAAAAT 
TTTGACTGGA AAGCTATTCA GGAAGGCGCA AACTCCATAG TAAGTAGTTT GCACCAAGCT 
GCTGCAGCCG CCGCGTGCTT ATCTAGACAA GCGTCATCCG ACTCAGATTC CATTCTGTCA 
CTAAAGTCCG GCATTTCTCT GGGATCGCCT TTTCATCTTA CACCTGATCA AGAGGAAAAG 
CCATTCACAA GCAATAAAGG CCCAAGAATT CTCAAACCTG GAGAGAAAAG CACATTAGAA 
GCAAAAAAAA TAGAATCTGA AAACAAAGGA ATCAAAGGCG GGAAAAAGGT TTATAAAAGC 
TTGATTACGG GAAAGATTCG CTCCAATTCA GAAATTTCCA GCCAAATGAA ACAACCCCTC 
CCGACAAACA TGCCTTCAAT CTCAAGAGGC AGGACGATGA TTCACATCCC AGGGCTTCGG 
AATAGCTCCT CTAGTACAAG CCCTGTCTCT AAGAAAGGCC CACCCCTCAA GACTCCAGCC 
TCTAAAAGCC CCAGTGAAGG GCCGGGAGCT ACCACTTCTC CTCGAGGAAC TAAGCCAGCA 
GGAAAGTCAG AGCTTAGCCC TATCACCAGG CAAACTTCCC AAATCAGTGG GTCAAATAAG 
GGGTCTTCTA GATCAGGATC TAGAGACTCC ACTCCCTCAA GACCTACACA GCAACCATTA 
AGTAGGCCAA TGCAGTCTCC AGGGCGAAAC TCAATTTCCC CTGGTAGAAA TGGAATAAGC 
CCTCCTAACA AACTGTCTCA GCTGCCCAGA ACATCATCTC CCAGTACTGC TTCAACTAAG 
TCCTCCGGTT CTGGGAAAAT GTCATATACA TCCCCAGGTA GACAGCTGAG CCAACAAAAT 
CTTACCAAAC AAGCAAGTTT ATCCAAGAAT GCCAGCAGTA TCCCCAGAAG TGAGTCGGCA 
TCTAAAGGAC TGAATCAGAT GAGTAACGGC AATGGGTCAA ATAAAAAGGT AGAACTTTCT 
AGAATGTCTT CAACTAAATC AAGTGGAAGT GAATCAGACA GATCAGAAAG GCCTGCATTA 
GTACGCCAGT CTACTTTCAT CAAAGAAGCC CCAAGCCCAA CCCTGAGGAG GAAACTGGAG 
GAATCTGCCT CATTTGAATC CCTTTCTCCA TCTTCTAGAC CAGATTCTCC CACCAGGTCG 
CAGGCACAGA CCCCAGTTTT AAGCCCTTCC CTTCCTGATA TGTCTCTGTC CACACATCCA 
TCTGTTCAGG CAGGTGGGTG GCGAAAGCTC CCGCCTAATC TCAGCCCCAC TATCGAGTAT 
AATGACGGAA GGCCCACAAA ACGGCATGAT ATTGCACGCT CCCATTCTGA AAGTCCTTCC 
AGACTACCAA TCAACCGGGC GGGAACCTGG AAGCGTGAAC ACAGCAAACA TTCCTCGTCC 
CTTCCTCGAG TGAGTACTTG GAGAAGAACT GGAAGCTCAT CTTCTATTCT TTCTGCTTCA 
TCAGAGTCCA GTGAAAAAGC AAAAAGTGAG GATGAAAGGC ATGTGAGCTC CATGCCAGCA 
CCCAGACAGA TGAAGGAAAA CCAGGTGCCC ACCAAAGGAA CATGGAGGAA AATCAAGGAA 
AGTGACATTT CTCCCACAGG CATGGCTTCT CAGAGCGCTT CCTCAGGTGC TGCCAGTGGT 
GCTGAATCCA AGCCTCTGAT CTATCAGATG GCACCTCCTG TCTCTAAAAC AGAGGATGTT 
TGGGTGAGAA TTGAGGACTG CCCCATTAAC AACCCTAGAT CTGGACGGTC CCCCACAGGC 
AACACCCCCC CAGTGATTGA CAGTGTTTCA GAGAAGGGAA GTTCAAGCAT TAAAGATTCA 
AAAGACACCC ATGGGAAACA GAGTGTGGGC AGTGGCAGTC CTGTGCAAAC CGTGGGTCTG 
GAAACCCGCC TCAACTCCTT TGTTCAGGTA GAGGCCCCAG AACAGAAAGG AACTGAGGCA 
AAACCAGGAC AGAGTAACCC AGTCTCTATA GCAGAGACTG CTGAGACGTG TATAGCAGAG 
CGTACCCCTT TCAGTTCCAG TAGCTCCAGC AAGCACAGCT CACCTAGCGG GACTGTTGCT 
GCCAGAGTGA CACCTTTTAA TTACAACCCT AGCCCTAGGA AGAGCAGCGC AGACAGCACT 
TCAGCCCGGC CGTCTCAGAT CCCTACGCCA GTGAGCACCA ACACGAAGAA GAGAGATTCG 
AAGACTGACA GCACAGAATC CAGTGGAGCC CAAAGTCCTA AACGCCATTC CGGGTCTTAC 
CTCGTGACGT CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250279.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106139.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X8, mRNA.

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CloneID	OMu87322
Clone ID Related Accession (Same CDS sequence)	XM_030250279.1
Accession Version	XM_030250279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGT ATTGGCGCAG CTTGCCGGGG TGTCACTGGA GACAGAATGG AGGTGCTGCC 
GGGCTCGAAA ATGGGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGGGGACTG CAGGCCATTG CAGAGTTATT GCAGGTGGAC 
TGTGAGATGT ATGGGCTTAC TAATGACCAC TACAGTGTTA CTTTAAGACG GTATGCTGGA 
ATGGCTTTGA CAAACTTGAC CTTTGGAGAT GTTGCCAACA AGGCTACGCT GTGTTCTATG 
AAAGGCTGCA TGAGAGCACT TGTGGCCCAG TTAAAATCTG AGAGTGAAGA CTTACAGCAG 
GTTATTGCAA GTGTTTTGAG GAATTTGTCT TGGCGAGCAG ATGTAAATAG CAAAAAGACG 
TTGAGAGAAG TTGGAAGTGT GAAAGCATTG ATGGAATGTG CTTTGGAAGT TAAAAAGGAA 
TCAACCCTCA AAAGCGTTTT GAGTGCCTTA TGGAACCTGT CTGCACACTG CACTGAGAAT 
AAGGCTGACA TCTGTGCTGT GGATGGAGCA CTGGCATTTC TGGTTGGCAC CCTCACTTAC 
CGGAGCCAGA CAAATACTTT AGCCATTATT GAAAGTGGAG GTGGGATATT ACGGAATGTG 
TCCAGCTTGA TAGCTACAAA CGAAGACCAC AGGCAAATCC TAAGAGAGAA CAATTGCCTA 
CAAACTTTAT TACAGCACTT GAAATCTCAC AGCTTGACAA TAGTCAGTAA TGCATGTGGA 
ACTTTGTGGA ATCTCTCAGC AAGAAATCCT AAAGACCAGG AAGCCTTGTG GGACATGGGG 
GCAGTGAGCA TGCTCAAGAA CCTCATTCAT TCCAAGCACA AAATGATTGC CATGGGAAGT 
GCAGCAGCTT TAAGGAATCT CATGGCAAAC AGACCTGCAA AGTATAAGGA TGCCAATATC 
ATGTCTCCCG GCTCAAGTCT GCCATCCCTT CACGTTAGGA AACAGAAAGC TCTAGAAGCT 
GAGCTAGATG CTCAGCATTT ATCAGAAACC TTCGACAACA TTGACAACCT AAGTCCCAAG 
GCCTCTCACC GGAGTAAGCA GAGACACAAG CAGAATCTTT ATGGTGACTA TGCTTTTGAC 
GCCAATCGAC ATGATGATAG TAGGTCAGAC AATTTCAATA CTGGAAACAT GACTGTTCTT 
TCACCATATT TAAATACTAC GGTATTGCCC AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC 
AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG GAGAGAGAGC GAGGTATTGG CCTCAGTGCT 
TACCATCCAA CAACAGAAAA TGCAGGAACC TCATCAAAAC GAGGTCTGCA GATCACTACC 
ACTGCAGCCC AGATAGCCAA AGTTATGGAA GAAGTATCAG CCATTCATAC CTCCCAGGAC 
GACAGAAGTT CTGCTTCTAC CACCGAGTTC CATTGTGTGG CAGACGACAG GAGTGCGGCA 
CGAAGAAGCT CTGCCTCCCA CACACACTCA AACACATACA ACTTCACTAA GTCGGAAAAT 
TCAAATAGGA CATGCTCTAT GCCTTATGCC AAAGTGGAAT ATAAACGATC TTCAAATGAC 
AGTTTAAATA GTGTCACTAG TAGTGATGGA TATGGTAAAA GAGGCCAAAT GAAACCCTCA 
GTTGAATCCT ATTCTGAAGA TGATGAAAGT AAATTTTGCA GTTATGGTCA GTATCCAGCT 
GACCTAGCCC ATAAGATACA CAGTGCAAAT CATATGGATG ATAATGATGG AGAACTGGAT 
ACACCAATAA ATTACAGTCT TAAATATTCA GATGAGCAGT TGAACTCAGG AAGGCAGAGT 
CCCTCACAGA ATGAAAGGTG GGCAAGACCA AAGCATGTGA TAGAAGATGA AATAAAGCAA 
AACGAGCAAA GACAAGCAAG AAGCCAGAAC ACCAGTTATC CTGTCTATTC TGAGAATACC 
GATGACAAAC ACCTCAAATT CCAACCACAT TTTGGACAAC AAGAATGTGT TTCCCCATAT 
AGGTCAAGGG GAACCAGTGG TTCAGAAACA AATCGAATGG GTTCTAGTCA TGCAATTAAT 
CAAAATGTAA ACCAGTCTCT GTGTCAGGAA GATGATTATG AAGATGATAA ACCTACCAAC 
TACAGTGAAC GTTATTCTGA GGAAGAACAA CATGAAGAAG AAGAAGAGAG ACCGACAAAT 
TATAGCATAA AATATAATGA AGAGAAACAT CATGTGGATC AGCCTATTGA TTATAGTTTA 
AAATATGCCA CTGACATTTC TTCCTCACAA AAACCATCAT TTTCATTCTC AAAGAATTCA 
TCAGCACAAA GCACTAAACC TGAACATCTC TCTCCAAGCA GCGAGAATAC AGCTGTACCT 
CCATCTAATG CCAAAAGGCA GAATCAGCTG CGTCCAAGTT CAGCACAAAG AAATGGCCAG 
ACTCAAAAAG GCACTACTTG CAAAGTCCCC TCCATCAACC AAGAAACAAT ACAGACTTAC 
TGCGTAGAAG ACACCCCAAT ATGTTTTTCA AGGTGCAGTT CATTATCATC ACTGTCATCA 
GCTGACGATG AAATAGGATG TGATCAGACA ACACAGGAAG CAGATTCTGC TAATACTCTG 
CAGACAGCAG AAGTAAAAGA GAATGATGTA ACTCGGTCAG CTGAAGATCC TGCAACTGAA 
GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC AAACCCAGCC GACTCCAGGC TTCTGGCTTA 
TCTTCAGAAT CAACCAGGCA TAATAAAGCT GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC 
TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT CCCCCAGAAC ACTATGTCCA GGAGACTCCG 
CTCGTATTCA GCAGGTGTAC TTCTGTCAGC TCCCTTGACA GTTTTGAGAG TCGCTCCATT 
GCCAGCTCTG TTCAGAGTGA GCCATGTAGT GGAATGGTGA GTGGCATCAT AAGCCCCAGT 
GACCTTCCAG ATAGTCCTGG GCAGACCATG CCACCAAGCA GAAGCAAAAC CCCTCCACCT 
CCTCCACAGA CAGTGCAGGC CAAGAGAGAG GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG 
AAGAGAGAGA GTGGGCCTAA GCAGACTGCT GTAAATGCTG CCGTGCAGAG GGTGCAGGTC 
CTTCCAGACG TGGATACTTT GTTACACTTC GCCACAGAAA GTACTCCAGA CGGGTTTTCT 
TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG GATGAGCCAT TTATACAGAA AGATGTAGAA 
TTAAGAATCA TGCCTCCAGT TCAGGAAAAC GACAATGGGA ATGAAACTGA ATCAGAACAG 
CCTGAGGAAT CAAATGAAAA CCAGGATAAA GAGGTAGAAA AGCCTGACTC TGAAAAAGAC 
TTATTAGATG ATTCTGATGA CGATGATATT GAAATATTAG AAGAATGTAT TATTTCAGCC 
ATGCCAACAA AGTCATCACG CAAAGCCAAA AAACTAGCCC AGACTGCTTC AAAATTACCT 
CCACCTGTGG CAAGGAAACC AAGTCAGCTA CCTGTGTATA AACTTCTGCC AGCACAGAAT 
AGGCTGCAGG CACAAAAACA TGTTAGCTTT ACACCAGGGG ATGATGTGCC CCGGGTGTAC 
TGTGTAGAAG GGACACCTAT AAACTTTTCC ACAGCAACGT CTCTAAGTGA TCTGACAATA 
GAGTCCCCTC CAAATGAATT GGCTACTGGA GATGGGGTCA GAGCGGGTAT ACAGTCAGGT 
GAATTTGAAA AACGAGATAC CATTCCTACA GAAGGCAGAA GTACAGATGA TGCTCAGCGA 
GGAAAAATCT CATCTATAGT TACACCAGAC CTGGATGACA ACAAAGCAGA GGAAGGAGAT 
ATTCTTGCAG AATGTATCAA TTCTGCTATG CCCAAAGGAA AAAGCCACAA GCCTTTCCGA 
GTGAAAAAGA TAATGGACCA AGTCCAACAA GCATCCTCGA CTTCATCTGG AGCTAACAAA 
AATCAAGTAG ACACTAAGAA AAAGAAGCCT ACTTCACCAG TAAAGCCCAT GCCACAAAAT 
ACTGAATATA GAACGCGTGT GAGAAAGAAT ACAGACTCAA AAGTTAATGT AAATACTGAA 
GAAACTTTCT CAGACAACAA AGACTCAAAG AAACCAAGCT TACAAACCAA TGCCAAGGCC 
TTCAATGAAA AGCTACCTAA CAATGAAGAC AGAGTGCGGG GGAGCTTCGC CTTGGACTCA 
CCGCATCACT ACACCCCTAT TGAGGGGACG CCGTACTGCT TTTCCCGAAA TGACTCCTTG 
AGTTCTCTGG ATTTTGATGA TGACGATGTT GACCTTTCCA GGGAAAAGGC CGAGTTAAGA 
AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC AAAGTTACCT GCCGCCCAGA ACCAAACTCA 
AGCCAGCAGG CAGCTAGTAA GTCACAAGCC AGTATAAAAC ATCCAGCAAA CAGAGCACAG 
TCCAAACCAG TGCTGCAGAA ACAGCCCACT TTCCCCCAGT CCTCCAAAGA CGGACCAGAT 
AGAGGGGCAG CAACTGACGA AAAACTGCAG AATTTTGCTA TTGAAAATAC TCCAGTTTGC 
TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT AGTGACATTG ACCAGGAAAA CAACAATAAC 
AAAGAAAGTG AACCAATCAA AGAAGCTGAA CCTGCCAACT CACAAGGAGA GCCCAGTAAG 
CCTCAGGCAT CCGGGTATGC TCCCAAGTCC TTCCACGTCG AAGACACCCC TGTCTGTTTC 
TCAAGAAACA GCTCTCTCAG TTCTCTTAGC ATTGACTCTG AGGACGACCT GTTACAGGAG 
TGTATAAGTT CTGCCATGCC AAAAAAGAAA AGGCCTTCAA GACTCAAGAG TGAGAGCGAA 
AAGCAGAGCC CTAGAAAAGT GGGTGGCATA TTAGCTGAAG ACCTGACGCT TGATTTGAAA 
GATCTACAGA GGCCAGATTC AGAACACGCT TTCTCCCCCG ACTCAGAAAA TTTTGACTGG 
AAAGCTATTC AGGAAGGCGC AAACTCCATA GTAAGTAGTT TGCACCAAGC TGCTGCAGCC 
GCCGCGTGCT TATCTAGACA AGCGTCATCC GACTCAGATT CCATTCTGTC ACTAAAGTCC 
GGCATTTCTC TGGGATCGCC TTTTCATCTT ACACCTGATC AAGAGGAAAA GCCATTCACA 
AGCAATAAAG GCCCAAGAAT TCTCAAACCT GGAGAGAAAA GCACATTAGA AGCAAAAAAA 
ATAGAATCTG AAAACAAAGG AATCAAAGGC GGGAAAAAGG TTTATAAAAG CTTGATTACG 
GGAAAGATTC GCTCCAATTC AGAAATTTCC AGCCAAATGA AACAACCCCT CCCGACAAAC 
ATGCCTTCAA TCTCAAGAGG CAGGACGATG ATTCACATCC CAGGGCTTCG GAATAGCTCC 
TCTAGTACAA GCCCTGTCTC TAAGAAAGGC CCACCCCTCA AGACTCCAGC CTCTAAAAGC 
CCCAGTGAAG GGCCGGGAGC TACCACTTCT CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA 
GAGCTTAGCC CTATCACCAG GCAAACTTCC CAAATCAGTG GGTCAAATAA GGGGTCTTCT 
AGATCAGGAT CTAGAGACTC CACTCCCTCA AGACCTACAC AGCAACCATT AAGTAGGCCA 
ATGCAGTCTC CAGGGCGAAA CTCAATTTCC CCTGGTAGAA ATGGAATAAG CCCTCCTAAC 
AAACTGTCTC AGCTGCCCAG AACATCATCT CCCAGTACTG CTTCAACTAA GTCCTCCGGT 
TCTGGGAAAA TGTCATATAC ATCCCCAGGT AGACAGCTGA GCCAACAAAA TCTTACCAAA 
CAAGCAAGTT TATCCAAGAA TGCCAGCAGT ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA 
CTGAATCAGA TGAGTAACGG CAATGGGTCA AATAAAAAGG TAGAACTTTC TAGAATGTCT 
TCAACTAAAT CAAGTGGAAG TGAATCAGAC AGATCAGAAA GGCCTGCATT AGTACGCCAG 
TCTACTTTCA TCAAAGAAGC CCCAAGCCCA ACCCTGAGGA GGAAACTGGA GGAATCTGCC 
TCATTTGAAT CCCTTTCTCC ATCTTCTAGA CCAGATTCTC CCACCAGGTC GCAGGCACAG 
ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT ATGTCTCTGT CCACACATCC ATCTGTTCAG 
GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT CTCAGCCCCA CTATCGAGTA TAATGACGGA 
AGGCCCACAA AACGGCATGA TATTGCACGC TCCCATTCTG AAAGTCCTTC CAGACTACCA 
ATCAACCGGG CGGGAACCTG GAAGCGTGAA CACAGCAAAC ATTCCTCGTC CCTTCCTCGA 
GTGAGTACTT GGAGAAGAAC TGGAAGCTCA TCTTCTATTC TTTCTGCTTC ATCAGAGTCC 
AGTGAAAAAG CAAAAAGTGA GGATGAAAGG CATGTGAGCT CCATGCCAGC ACCCAGACAG 
ATGAAGGAAA ACCAGGTGCC CACCAAAGGA ACATGGAGGA AAATCAAGGA AAGTGACATT 
TCTCCCACAG GCATGGCTTC TCAGAGCGCT TCCTCAGGTG CTGCCAGTGG TGCTGAATCC 
AAGCCTCTGA TCTATCAGAT GGCACCTCCT GTCTCTAAAA CAGAGGATGT TTGGGTGAGA 
ATTGAGGACT GCCCCATTAA CAACCCTAGA TCTGGACGGT CCCCCACAGG CAACACCCCC 
CCAGTGATTG ACAGTGTTTC AGAGAAGGGA AGTTCAAGCA TTAAAGATTC AAAAGACACC 
CATGGGAAAC AGAGTGTGGG CAGTGGCAGT CCTGTGCAAA CCGTGGGTCT GGAAACCCGC 
CTCAACTCCT TTGTTCAGGT AGAGGCCCCA GAACAGAAAG GAACTGAGGC AAAACCAGGA 
CAGAGTAACC CAGTCTCTAT AGCAGAGACT GCTGAGACGT GTATAGCAGA GCGTACCCCT 
TTCAGTTCCA GTAGCTCCAG CAAGCACAGC TCACCTAGCG GGACTGTTGC TGCCAGAGTG 
ACACCTTTTA ATTACAACCC TAGCCCTAGG AAGAGCAGCG CAGACAGCAC TTCAGCCCGG 
CCGTCTCAGA TCCCTACGCC AGTGAGCACC AACACGAAGA AGAGAGATTC GAAGACTGAC 
AGCACAGAAT CCAGTGGAGC CCAAAGTCCT AAACGCCATT CCGGGTCTTA CCTCGTGACG 
TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106139.1
CDS	133..8601
Translation	MWLYWRSLPGCHWRQNGGAAGLENGEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250279.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250279.1

ORF Insert Sequence:

ATGTGGCTGT ATTGGCGCAG CTTGCCGGGG TGTCACTGGA GACAGAATGG AGGTGCTGCC 
GGGCTCGAAA ATGGGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGGTG GAAATGGTGT ATTCCTTGTT GTCAATGCTT 
GGTACTCATG ATAAGGACGA TATGTCACGA ACTTTGCTAG CTATGTCCAG CTCCCAAGAC 
AGCTGTATAT CCATGCGGCA GTCTGGATGT CTTCCTCTCC TCATCCAGCT TTTACATGGC 
AATGACAAAG ACTCTGTATT GTTGGGAAAT TCCCGGGGCA GTAAAGAGGC TCGGGCCAGG 
GCCAGTGCAG CACTCCACAA CATCATTCAC TCACAGCCTG ATGACAAGAG AGGCAGGCGT 
GAAATCCGAG TCCTTCATCT TTTGGAACAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGGGGACTG CAGGCCATTG CAGAGTTATT GCAGGTGGAC 
TGTGAGATGT ATGGGCTTAC TAATGACCAC TACAGTGTTA CTTTAAGACG GTATGCTGGA 
ATGGCTTTGA CAAACTTGAC CTTTGGAGAT GTTGCCAACA AGGCTACGCT GTGTTCTATG 
AAAGGCTGCA TGAGAGCACT TGTGGCCCAG TTAAAATCTG AGAGTGAAGA CTTACAGCAG 
GTTATTGCAA GTGTTTTGAG GAATTTGTCT TGGCGAGCAG ATGTAAATAG CAAAAAGACG 
TTGAGAGAAG TTGGAAGTGT GAAAGCATTG ATGGAATGTG CTTTGGAAGT TAAAAAGGAA 
TCAACCCTCA AAAGCGTTTT GAGTGCCTTA TGGAACCTGT CTGCACACTG CACTGAGAAT 
AAGGCTGACA TCTGTGCTGT GGATGGAGCA CTGGCATTTC TGGTTGGCAC CCTCACTTAC 
CGGAGCCAGA CAAATACTTT AGCCATTATT GAAAGTGGAG GTGGGATATT ACGGAATGTG 
TCCAGCTTGA TAGCTACAAA CGAAGACCAC AGGCAAATCC TAAGAGAGAA CAATTGCCTA 
CAAACTTTAT TACAGCACTT GAAATCTCAC AGCTTGACAA TAGTCAGTAA TGCATGTGGA 
ACTTTGTGGA ATCTCTCAGC AAGAAATCCT AAAGACCAGG AAGCCTTGTG GGACATGGGG 
GCAGTGAGCA TGCTCAAGAA CCTCATTCAT TCCAAGCACA AAATGATTGC CATGGGAAGT 
GCAGCAGCTT TAAGGAATCT CATGGCAAAC AGACCTGCAA AGTATAAGGA TGCCAATATC 
ATGTCTCCCG GCTCAAGTCT GCCATCCCTT CACGTTAGGA AACAGAAAGC TCTAGAAGCT 
GAGCTAGATG CTCAGCATTT ATCAGAAACC TTCGACAACA TTGACAACCT AAGTCCCAAG 
GCCTCTCACC GGAGTAAGCA GAGACACAAG CAGAATCTTT ATGGTGACTA TGCTTTTGAC 
GCCAATCGAC ATGATGATAG TAGGTCAGAC AATTTCAATA CTGGAAACAT GACTGTTCTT 
TCACCATATT TAAATACTAC GGTATTGCCC AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC 
AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG GAGAGAGAGC GAGGTATTGG CCTCAGTGCT 
TACCATCCAA CAACAGAAAA TGCAGGAACC TCATCAAAAC GAGGTCTGCA GATCACTACC 
ACTGCAGCCC AGATAGCCAA AGTTATGGAA GAAGTATCAG CCATTCATAC CTCCCAGGAC 
GACAGAAGTT CTGCTTCTAC CACCGAGTTC CATTGTGTGG CAGACGACAG GAGTGCGGCA 
CGAAGAAGCT CTGCCTCCCA CACACACTCA AACACATACA ACTTCACTAA GTCGGAAAAT 
TCAAATAGGA CATGCTCTAT GCCTTATGCC AAAGTGGAAT ATAAACGATC TTCAAATGAC 
AGTTTAAATA GTGTCACTAG TAGTGATGGA TATGGTAAAA GAGGCCAAAT GAAACCCTCA 
GTTGAATCCT ATTCTGAAGA TGATGAAAGT AAATTTTGCA GTTATGGTCA GTATCCAGCT 
GACCTAGCCC ATAAGATACA CAGTGCAAAT CATATGGATG ATAATGATGG AGAACTGGAT 
ACACCAATAA ATTACAGTCT TAAATATTCA GATGAGCAGT TGAACTCAGG AAGGCAGAGT 
CCCTCACAGA ATGAAAGGTG GGCAAGACCA AAGCATGTGA TAGAAGATGA AATAAAGCAA 
AACGAGCAAA GACAAGCAAG AAGCCAGAAC ACCAGTTATC CTGTCTATTC TGAGAATACC 
GATGACAAAC ACCTCAAATT CCAACCACAT TTTGGACAAC AAGAATGTGT TTCCCCATAT 
AGGTCAAGGG GAACCAGTGG TTCAGAAACA AATCGAATGG GTTCTAGTCA TGCAATTAAT 
CAAAATGTAA ACCAGTCTCT GTGTCAGGAA GATGATTATG AAGATGATAA ACCTACCAAC 
TACAGTGAAC GTTATTCTGA GGAAGAACAA CATGAAGAAG AAGAAGAGAG ACCGACAAAT 
TATAGCATAA AATATAATGA AGAGAAACAT CATGTGGATC AGCCTATTGA TTATAGTTTA 
AAATATGCCA CTGACATTTC TTCCTCACAA AAACCATCAT TTTCATTCTC AAAGAATTCA 
TCAGCACAAA GCACTAAACC TGAACATCTC TCTCCAAGCA GCGAGAATAC AGCTGTACCT 
CCATCTAATG CCAAAAGGCA GAATCAGCTG CGTCCAAGTT CAGCACAAAG AAATGGCCAG 
ACTCAAAAAG GCACTACTTG CAAAGTCCCC TCCATCAACC AAGAAACAAT ACAGACTTAC 
TGCGTAGAAG ACACCCCAAT ATGTTTTTCA AGGTGCAGTT CATTATCATC ACTGTCATCA 
GCTGACGATG AAATAGGATG TGATCAGACA ACACAGGAAG CAGATTCTGC TAATACTCTG 
CAGACAGCAG AAGTAAAAGA GAATGATGTA ACTCGGTCAG CTGAAGATCC TGCAACTGAA 
GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC AAACCCAGCC GACTCCAGGC TTCTGGCTTA 
TCTTCAGAAT CAACCAGGCA TAATAAAGCT GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC 
TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT CCCCCAGAAC ACTATGTCCA GGAGACTCCG 
CTCGTATTCA GCAGGTGTAC TTCTGTCAGC TCCCTTGACA GTTTTGAGAG TCGCTCCATT 
GCCAGCTCTG TTCAGAGTGA GCCATGTAGT GGAATGGTGA GTGGCATCAT AAGCCCCAGT 
GACCTTCCAG ATAGTCCTGG GCAGACCATG CCACCAAGCA GAAGCAAAAC CCCTCCACCT 
CCTCCACAGA CAGTGCAGGC CAAGAGAGAG GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG 
AAGAGAGAGA GTGGGCCTAA GCAGACTGCT GTAAATGCTG CCGTGCAGAG GGTGCAGGTC 
CTTCCAGACG TGGATACTTT GTTACACTTC GCCACAGAAA GTACTCCAGA CGGGTTTTCT 
TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG GATGAGCCAT TTATACAGAA AGATGTAGAA 
TTAAGAATCA TGCCTCCAGT TCAGGAAAAC GACAATGGGA ATGAAACTGA ATCAGAACAG 
CCTGAGGAAT CAAATGAAAA CCAGGATAAA GAGGTAGAAA AGCCTGACTC TGAAAAAGAC 
TTATTAGATG ATTCTGATGA CGATGATATT GAAATATTAG AAGAATGTAT TATTTCAGCC 
ATGCCAACAA AGTCATCACG CAAAGCCAAA AAACTAGCCC AGACTGCTTC AAAATTACCT 
CCACCTGTGG CAAGGAAACC AAGTCAGCTA CCTGTGTATA AACTTCTGCC AGCACAGAAT 
AGGCTGCAGG CACAAAAACA TGTTAGCTTT ACACCAGGGG ATGATGTGCC CCGGGTGTAC 
TGTGTAGAAG GGACACCTAT AAACTTTTCC ACAGCAACGT CTCTAAGTGA TCTGACAATA 
GAGTCCCCTC CAAATGAATT GGCTACTGGA GATGGGGTCA GAGCGGGTAT ACAGTCAGGT 
GAATTTGAAA AACGAGATAC CATTCCTACA GAAGGCAGAA GTACAGATGA TGCTCAGCGA 
GGAAAAATCT CATCTATAGT TACACCAGAC CTGGATGACA ACAAAGCAGA GGAAGGAGAT 
ATTCTTGCAG AATGTATCAA TTCTGCTATG CCCAAAGGAA AAAGCCACAA GCCTTTCCGA 
GTGAAAAAGA TAATGGACCA AGTCCAACAA GCATCCTCGA CTTCATCTGG AGCTAACAAA 
AATCAAGTAG ACACTAAGAA AAAGAAGCCT ACTTCACCAG TAAAGCCCAT GCCACAAAAT 
ACTGAATATA GAACGCGTGT GAGAAAGAAT ACAGACTCAA AAGTTAATGT AAATACTGAA 
GAAACTTTCT CAGACAACAA AGACTCAAAG AAACCAAGCT TACAAACCAA TGCCAAGGCC 
TTCAATGAAA AGCTACCTAA CAATGAAGAC AGAGTGCGGG GGAGCTTCGC CTTGGACTCA 
CCGCATCACT ACACCCCTAT TGAGGGGACG CCGTACTGCT TTTCCCGAAA TGACTCCTTG 
AGTTCTCTGG ATTTTGATGA TGACGATGTT GACCTTTCCA GGGAAAAGGC CGAGTTAAGA 
AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC AAAGTTACCT GCCGCCCAGA ACCAAACTCA 
AGCCAGCAGG CAGCTAGTAA GTCACAAGCC AGTATAAAAC ATCCAGCAAA CAGAGCACAG 
TCCAAACCAG TGCTGCAGAA ACAGCCCACT TTCCCCCAGT CCTCCAAAGA CGGACCAGAT 
AGAGGGGCAG CAACTGACGA AAAACTGCAG AATTTTGCTA TTGAAAATAC TCCAGTTTGC 
TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT AGTGACATTG ACCAGGAAAA CAACAATAAC 
AAAGAAAGTG AACCAATCAA AGAAGCTGAA CCTGCCAACT CACAAGGAGA GCCCAGTAAG 
CCTCAGGCAT CCGGGTATGC TCCCAAGTCC TTCCACGTCG AAGACACCCC TGTCTGTTTC 
TCAAGAAACA GCTCTCTCAG TTCTCTTAGC ATTGACTCTG AGGACGACCT GTTACAGGAG 
TGTATAAGTT CTGCCATGCC AAAAAAGAAA AGGCCTTCAA GACTCAAGAG TGAGAGCGAA 
AAGCAGAGCC CTAGAAAAGT GGGTGGCATA TTAGCTGAAG ACCTGACGCT TGATTTGAAA 
GATCTACAGA GGCCAGATTC AGAACACGCT TTCTCCCCCG ACTCAGAAAA TTTTGACTGG 
AAAGCTATTC AGGAAGGCGC AAACTCCATA GTAAGTAGTT TGCACCAAGC TGCTGCAGCC 
GCCGCGTGCT TATCTAGACA AGCGTCATCC GACTCAGATT CCATTCTGTC ACTAAAGTCC 
GGCATTTCTC TGGGATCGCC TTTTCATCTT ACACCTGATC AAGAGGAAAA GCCATTCACA 
AGCAATAAAG GCCCAAGAAT TCTCAAACCT GGAGAGAAAA GCACATTAGA AGCAAAAAAA 
ATAGAATCTG AAAACAAAGG AATCAAAGGC GGGAAAAAGG TTTATAAAAG CTTGATTACG 
GGAAAGATTC GCTCCAATTC AGAAATTTCC AGCCAAATGA AACAACCCCT CCCGACAAAC 
ATGCCTTCAA TCTCAAGAGG CAGGACGATG ATTCACATCC CAGGGCTTCG GAATAGCTCC 
TCTAGTACAA GCCCTGTCTC TAAGAAAGGC CCACCCCTCA AGACTCCAGC CTCTAAAAGC 
CCCAGTGAAG GGCCGGGAGC TACCACTTCT CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA 
GAGCTTAGCC CTATCACCAG GCAAACTTCC CAAATCAGTG GGTCAAATAA GGGGTCTTCT 
AGATCAGGAT CTAGAGACTC CACTCCCTCA AGACCTACAC AGCAACCATT AAGTAGGCCA 
ATGCAGTCTC CAGGGCGAAA CTCAATTTCC CCTGGTAGAA ATGGAATAAG CCCTCCTAAC 
AAACTGTCTC AGCTGCCCAG AACATCATCT CCCAGTACTG CTTCAACTAA GTCCTCCGGT 
TCTGGGAAAA TGTCATATAC ATCCCCAGGT AGACAGCTGA GCCAACAAAA TCTTACCAAA 
CAAGCAAGTT TATCCAAGAA TGCCAGCAGT ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA 
CTGAATCAGA TGAGTAACGG CAATGGGTCA AATAAAAAGG TAGAACTTTC TAGAATGTCT 
TCAACTAAAT CAAGTGGAAG TGAATCAGAC AGATCAGAAA GGCCTGCATT AGTACGCCAG 
TCTACTTTCA TCAAAGAAGC CCCAAGCCCA ACCCTGAGGA GGAAACTGGA GGAATCTGCC 
TCATTTGAAT CCCTTTCTCC ATCTTCTAGA CCAGATTCTC CCACCAGGTC GCAGGCACAG 
ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT ATGTCTCTGT CCACACATCC ATCTGTTCAG 
GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT CTCAGCCCCA CTATCGAGTA TAATGACGGA 
AGGCCCACAA AACGGCATGA TATTGCACGC TCCCATTCTG AAAGTCCTTC CAGACTACCA 
ATCAACCGGG CGGGAACCTG GAAGCGTGAA CACAGCAAAC ATTCCTCGTC CCTTCCTCGA 
GTGAGTACTT GGAGAAGAAC TGGAAGCTCA TCTTCTATTC TTTCTGCTTC ATCAGAGTCC 
AGTGAAAAAG CAAAAAGTGA GGATGAAAGG CATGTGAGCT CCATGCCAGC ACCCAGACAG 
ATGAAGGAAA ACCAGGTGCC CACCAAAGGA ACATGGAGGA AAATCAAGGA AAGTGACATT 
TCTCCCACAG GCATGGCTTC TCAGAGCGCT TCCTCAGGTG CTGCCAGTGG TGCTGAATCC 
AAGCCTCTGA TCTATCAGAT GGCACCTCCT GTCTCTAAAA CAGAGGATGT TTGGGTGAGA 
ATTGAGGACT GCCCCATTAA CAACCCTAGA TCTGGACGGT CCCCCACAGG CAACACCCCC 
CCAGTGATTG ACAGTGTTTC AGAGAAGGGA AGTTCAAGCA TTAAAGATTC AAAAGACACC 
CATGGGAAAC AGAGTGTGGG CAGTGGCAGT CCTGTGCAAA CCGTGGGTCT GGAAACCCGC 
CTCAACTCCT TTGTTCAGGT AGAGGCCCCA GAACAGAAAG GAACTGAGGC AAAACCAGGA 
CAGAGTAACC CAGTCTCTAT AGCAGAGACT GCTGAGACGT GTATAGCAGA GCGTACCCCT 
TTCAGTTCCA GTAGCTCCAG CAAGCACAGC TCACCTAGCG GGACTGTTGC TGCCAGAGTG 
ACACCTTTTA ATTACAACCC TAGCCCTAGG AAGAGCAGCG CAGACAGCAC TTCAGCCCGG 
CCGTCTCAGA TCCCTACGCC AGTGAGCACC AACACGAAGA AGAGAGATTC GAAGACTGAC 
AGCACAGAAT CCAGTGGAGC CCAAAGTCCT AAACGCCATT CCGGGTCTTA CCTCGTGACG 
TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250280.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106140.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X9, mRNA.

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CloneID	OMu87323
Clone ID Related Accession (Same CDS sequence)	XM_030250280.1
Accession Version	XM_030250280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGTCCAG CTACACGTGT GGATCACGAA 
ACAGCCAGTG TTTTGAGTTC TAGCGGCACG CACTCTGCTC CTCGAAGGTT GACAAGTCAT 
CTGGGGACAA AGGTGGAAAT GGTGTATTCC TTGTTGTCAA TGCTTGGTAC TCATGATAAG 
GACGATATGT CACGAACTTT GCTAGCTATG TCCAGCTCCC AAGACAGCTG TATATCCATG 
CGGCAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT 
GTATTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC 
CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT 
CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAC 
GAACAAGGCA TGGACCAGGA CAAAAACCCA ATGCCAGCTC CTGTTGAGCA TCAGATCTGT 
CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA TTTGATGAAG AGCATAGGCA TGCAATGAAT 
GAACTTGGGG GACTGCAGGC CATTGCAGAG TTATTGCAGG TGGACTGTGA GATGTATGGG 
CTTACTAATG ACCACTACAG TGTTACTTTA AGACGGTATG CTGGAATGGC TTTGACAAAC 
TTGACCTTTG GAGATGTTGC CAACAAGGCT ACGCTGTGTT CTATGAAAGG CTGCATGAGA 
GCACTTGTGG CCCAGTTAAA ATCTGAGAGT GAAGACTTAC AGCAGGTTAT TGCAAGTGTT 
TTGAGGAATT TGTCTTGGCG AGCAGATGTA AATAGCAAAA AGACGTTGAG AGAAGTTGGA 
AGTGTGAAAG CATTGATGGA ATGTGCTTTG GAAGTTAAAA AGGAATCAAC CCTCAAAAGC 
GTTTTGAGTG CCTTATGGAA CCTGTCTGCA CACTGCACTG AGAATAAGGC TGACATCTGT 
GCTGTGGATG GAGCACTGGC ATTTCTGGTT GGCACCCTCA CTTACCGGAG CCAGACAAAT 
ACTTTAGCCA TTATTGAAAG TGGAGGTGGG ATATTACGGA ATGTGTCCAG CTTGATAGCT 
ACAAACGAAG ACCACAGGCA AATCCTAAGA GAGAACAATT GCCTACAAAC TTTATTACAG 
CACTTGAAAT CTCACAGCTT GACAATAGTC AGTAATGCAT GTGGAACTTT GTGGAATCTC 
TCAGCAAGAA ATCCTAAAGA CCAGGAAGCC TTGTGGGACA TGGGGGCAGT GAGCATGCTC 
AAGAACCTCA TTCATTCCAA GCACAAAATG ATTGCCATGG GAAGTGCAGC AGCTTTAAGG 
AATCTCATGG CAAACAGACC TGCAAAGTAT AAGGATGCCA ATATCATGTC TCCCGGCTCA 
AGTCTGCCAT CCCTTCACGT TAGGAAACAG AAAGCTCTAG AAGCTGAGCT AGATGCTCAG 
CATTTATCAG AAACCTTCGA CAACATTGAC AACCTAAGTC CCAAGGCCTC TCACCGGAGT 
AAGCAGAGAC ACAAGCAGAA TCTTTATGGT GACTATGCTT TTGACGCCAA TCGACATGAT 
GATAGTAGGT CAGACAATTT CAATACTGGA AACATGACTG TTCTTTCACC ATATTTAAAT 
ACTACGGTAT TGCCCAGCTC TTCTTCCTCA AGGGGAAGTT TAGACAGTTC TCGTTCTGAG 
AAAGACAGAA GTTTGGAGAG AGAGCGAGGT ATTGGCCTCA GTGCTTACCA TCCAACAACA 
GAAAATGCAG GAACCTCATC AAAACGAGGT CTGCAGATCA CTACCACTGC AGCCCAGATA 
GCCAAAGTTA TGGAAGAAGT ATCAGCCATT CATACCTCCC AGGACGACAG AAGTTCTGCT 
TCTACCACCG AGTTCCATTG TGTGGCAGAC GACAGGAGTG CGGCACGAAG AAGCTCTGCC 
TCCCACACAC ACTCAAACAC ATACAACTTC ACTAAGTCGG AAAATTCAAA TAGGACATGC 
TCTATGCCTT ATGCCAAAGT GGAATATAAA CGATCTTCAA ATGACAGTTT AAATAGTGTC 
ACTAGTAGTG ATGGATATGG TAAAAGAGGC CAAATGAAAC CCTCAGTTGA ATCCTATTCT 
GAAGATGATG AAAGTAAATT TTGCAGTTAT GGTCAGTATC CAGCTGACCT AGCCCATAAG 
ATACACAGTG CAAATCATAT GGATGATAAT GATGGAGAAC TGGATACACC AATAAATTAC 
AGTCTTAAAT ATTCAGATGA GCAGTTGAAC TCAGGAAGGC AGAGTCCCTC ACAGAATGAA 
AGGTGGGCAA GACCAAAGCA TGTGATAGAA GATGAAATAA AGCAAAACGA GCAAAGACAA 
GCAAGAAGCC AGAACACCAG TTATCCTGTC TATTCTGAGA ATACCGATGA CAAACACCTC 
AAATTCCAAC CACATTTTGG ACAACAAGAA TGTGTTTCCC CATATAGGTC AAGGGGAACC 
AGTGGTTCAG AAACAAATCG AATGGGTTCT AGTCATGCAA TTAATCAAAA TGTAAACCAG 
TCTCTGTGTC AGGAAGATGA TTATGAAGAT GATAAACCTA CCAACTACAG TGAACGTTAT 
TCTGAGGAAG AACAACATGA AGAAGAAGAA GAGAGACCGA CAAATTATAG CATAAAATAT 
AATGAAGAGA AACATCATGT GGATCAGCCT ATTGATTATA GTTTAAAATA TGCCACTGAC 
ATTTCTTCCT CACAAAAACC ATCATTTTCA TTCTCAAAGA ATTCATCAGC ACAAAGCACT 
AAACCTGAAC ATCTCTCTCC AAGCAGCGAG AATACAGCTG TACCTCCATC TAATGCCAAA 
AGGCAGAATC AGCTGCGTCC AAGTTCAGCA CAAAGAAATG GCCAGACTCA AAAAGGCACT 
ACTTGCAAAG TCCCCTCCAT CAACCAAGAA ACAATACAGA CTTACTGCGT AGAAGACACC 
CCAATATGTT TTTCAAGGTG CAGTTCATTA TCATCACTGT CATCAGCTGA CGATGAAATA 
GGATGTGATC AGACAACACA GGAAGCAGAT TCTGCTAATA CTCTGCAGAC AGCAGAAGTA 
AAAGAGAATG ATGTAACTCG GTCAGCTGAA GATCCTGCAA CTGAAGTTCC AGCAGTGTCC 
CAGAATGCTA GAGCCAAACC CAGCCGACTC CAGGCTTCTG GCTTATCTTC AGAATCAACC 
AGGCATAATA AAGCTGTTGA GTTTTCTTCA GGAGCCAAGT CTCCCTCCAA AAGTGGTGCT 
CAGACACCCA AAAGTCCCCC AGAACACTAT GTCCAGGAGA CTCCGCTCGT ATTCAGCAGG 
TGTACTTCTG TCAGCTCCCT TGACAGTTTT GAGAGTCGCT CCATTGCCAG CTCTGTTCAG 
AGTGAGCCAT GTAGTGGAAT GGTGAGTGGC ATCATAAGCC CCAGTGACCT TCCAGATAGT 
CCTGGGCAGA CCATGCCACC AAGCAGAAGC AAAACCCCTC CACCTCCTCC ACAGACAGTG 
CAGGCCAAGA GAGAGGTGCC AAAAAGTAAA GTCCCTGCTG CTGAGAAGAG AGAGAGTGGG 
CCTAAGCAGA CTGCTGTAAA TGCTGCCGTG CAGAGGGTGC AGGTCCTTCC AGACGTGGAT 
ACTTTGTTAC ACTTCGCCAC AGAAAGTACT CCAGACGGGT TTTCTTGTTC CTCCAGCCTA 
AGTGCTCTGA GCCTGGATGA GCCATTTATA CAGAAAGATG TAGAATTAAG AATCATGCCT 
CCAGTTCAGG AAAACGACAA TGGGAATGAA ACTGAATCAG AACAGCCTGA GGAATCAAAT 
GAAAACCAGG ATAAAGAGGT AGAAAAGCCT GACTCTGAAA AAGACTTATT AGATGATTCT 
GATGACGATG ATATTGAAAT ATTAGAAGAA TGTATTATTT CAGCCATGCC AACAAAGTCA 
TCACGCAAAG CCAAAAAACT AGCCCAGACT GCTTCAAAAT TACCTCCACC TGTGGCAAGG 
AAACCAAGTC AGCTACCTGT GTATAAACTT CTGCCAGCAC AGAATAGGCT GCAGGCACAA 
AAACATGTTA GCTTTACACC AGGGGATGAT GTGCCCCGGG TGTACTGTGT AGAAGGGACA 
CCTATAAACT TTTCCACAGC AACGTCTCTA AGTGATCTGA CAATAGAGTC CCCTCCAAAT 
GAATTGGCTA CTGGAGATGG GGTCAGAGCG GGTATACAGT CAGGTGAATT TGAAAAACGA 
GATACCATTC CTACAGAAGG CAGAAGTACA GATGATGCTC AGCGAGGAAA AATCTCATCT 
ATAGTTACAC CAGACCTGGA TGACAACAAA GCAGAGGAAG GAGATATTCT TGCAGAATGT 
ATCAATTCTG CTATGCCCAA AGGAAAAAGC CACAAGCCTT TCCGAGTGAA AAAGATAATG 
GACCAAGTCC AACAAGCATC CTCGACTTCA TCTGGAGCTA ACAAAAATCA AGTAGACACT 
AAGAAAAAGA AGCCTACTTC ACCAGTAAAG CCCATGCCAC AAAATACTGA ATATAGAACG 
CGTGTGAGAA AGAATACAGA CTCAAAAGTT AATGTAAATA CTGAAGAAAC TTTCTCAGAC 
AACAAAGACT CAAAGAAACC AAGCTTACAA ACCAATGCCA AGGCCTTCAA TGAAAAGCTA 
CCTAACAATG AAGACAGAGT GCGGGGGAGC TTCGCCTTGG ACTCACCGCA TCACTACACC 
CCTATTGAGG GGACGCCGTA CTGCTTTTCC CGAAATGACT CCTTGAGTTC TCTGGATTTT 
GATGATGACG ATGTTGACCT TTCCAGGGAA AAGGCCGAGT TAAGAAAGGG CAAAGAAAGC 
AAGGATTCCG AAGCCAAAGT TACCTGCCGC CCAGAACCAA ACTCAAGCCA GCAGGCAGCT 
AGTAAGTCAC AAGCCAGTAT AAAACATCCA GCAAACAGAG CACAGTCCAA ACCAGTGCTG 
CAGAAACAGC CCACTTTCCC CCAGTCCTCC AAAGACGGAC CAGATAGAGG GGCAGCAACT 
GACGAAAAAC TGCAGAATTT TGCTATTGAA AATACTCCAG TTTGCTTTTC TCGAAATTCC 
TCTCTGAGTT CCCTTAGTGA CATTGACCAG GAAAACAACA ATAACAAAGA AAGTGAACCA 
ATCAAAGAAG CTGAACCTGC CAACTCACAA GGAGAGCCCA GTAAGCCTCA GGCATCCGGG 
TATGCTCCCA AGTCCTTCCA CGTCGAAGAC ACCCCTGTCT GTTTCTCAAG AAACAGCTCT 
CTCAGTTCTC TTAGCATTGA CTCTGAGGAC GACCTGTTAC AGGAGTGTAT AAGTTCTGCC 
ATGCCAAAAA AGAAAAGGCC TTCAAGACTC AAGAGTGAGA GCGAAAAGCA GAGCCCTAGA 
AAAGTGGGTG GCATATTAGC TGAAGACCTG ACGCTTGATT TGAAAGATCT ACAGAGGCCA 
GATTCAGAAC ACGCTTTCTC CCCCGACTCA GAAAATTTTG ACTGGAAAGC TATTCAGGAA 
GGCGCAAACT CCATAGTAAG TAGTTTGCAC CAAGCTGCTG CAGCCGCCGC GTGCTTATCT 
AGACAAGCGT CATCCGACTC AGATTCCATT CTGTCACTAA AGTCCGGCAT TTCTCTGGGA 
TCGCCTTTTC ATCTTACACC TGATCAAGAG GAAAAGCCAT TCACAAGCAA TAAAGGCCCA 
AGAATTCTCA AACCTGGAGA GAAAAGCACA TTAGAAGCAA AAAAAATAGA ATCTGAAAAC 
AAAGGAATCA AAGGCGGGAA AAAGGTTTAT AAAAGCTTGA TTACGGGAAA GATTCGCTCC 
AATTCAGAAA TTTCCAGCCA AATGAAACAA CCCCTCCCGA CAAACATGCC TTCAATCTCA 
AGAGGCAGGA CGATGATTCA CATCCCAGGG CTTCGGAATA GCTCCTCTAG TACAAGCCCT 
GTCTCTAAGA AAGGCCCACC CCTCAAGACT CCAGCCTCTA AAAGCCCCAG TGAAGGGCCG 
GGAGCTACCA CTTCTCCTCG AGGAACTAAG CCAGCAGGAA AGTCAGAGCT TAGCCCTATC 
ACCAGGCAAA CTTCCCAAAT CAGTGGGTCA AATAAGGGGT CTTCTAGATC AGGATCTAGA 
GACTCCACTC CCTCAAGACC TACACAGCAA CCATTAAGTA GGCCAATGCA GTCTCCAGGG 
CGAAACTCAA TTTCCCCTGG TAGAAATGGA ATAAGCCCTC CTAACAAACT GTCTCAGCTG 
CCCAGAACAT CATCTCCCAG TACTGCTTCA ACTAAGTCCT CCGGTTCTGG GAAAATGTCA 
TATACATCCC CAGGTAGACA GCTGAGCCAA CAAAATCTTA CCAAACAAGC AAGTTTATCC 
AAGAATGCCA GCAGTATCCC CAGAAGTGAG TCGGCATCTA AAGGACTGAA TCAGATGAGT 
AACGGCAATG GGTCAAATAA AAAGGTAGAA CTTTCTAGAA TGTCTTCAAC TAAATCAAGT 
GGAAGTGAAT CAGACAGATC AGAAAGGCCT GCATTAGTAC GCCAGTCTAC TTTCATCAAA 
GAAGCCCCAA GCCCAACCCT GAGGAGGAAA CTGGAGGAAT CTGCCTCATT TGAATCCCTT 
TCTCCATCTT CTAGACCAGA TTCTCCCACC AGGTCGCAGG CACAGACCCC AGTTTTAAGC 
CCTTCCCTTC CTGATATGTC TCTGTCCACA CATCCATCTG TTCAGGCAGG TGGGTGGCGA 
AAGCTCCCGC CTAATCTCAG CCCCACTATC GAGTATAATG ACGGAAGGCC CACAAAACGG 
CATGATATTG CACGCTCCCA TTCTGAAAGT CCTTCCAGAC TACCAATCAA CCGGGCGGGA 
ACCTGGAAGC GTGAACACAG CAAACATTCC TCGTCCCTTC CTCGAGTGAG TACTTGGAGA 
AGAACTGGAA GCTCATCTTC TATTCTTTCT GCTTCATCAG AGTCCAGTGA AAAAGCAAAA 
AGTGAGGATG AAAGGCATGT GAGCTCCATG CCAGCACCCA GACAGATGAA GGAAAACCAG 
GTGCCCACCA AAGGAACATG GAGGAAAATC AAGGAAAGTG ACATTTCTCC CACAGGCATG 
GCTTCTCAGA GCGCTTCCTC AGGTGCTGCC AGTGGTGCTG AATCCAAGCC TCTGATCTAT 
CAGATGGCAC CTCCTGTCTC TAAAACAGAG GATGTTTGGG TGAGAATTGA GGACTGCCCC 
ATTAACAACC CTAGATCTGG ACGGTCCCCC ACAGGCAACA CCCCCCCAGT GATTGACAGT 
GTTTCAGAGA AGGGAAGTTC AAGCATTAAA GATTCAAAAG ACACCCATGG GAAACAGAGT 
GTGGGCAGTG GCAGTCCTGT GCAAACCGTG GGTCTGGAAA CCCGCCTCAA CTCCTTTGTT 
CAGGTAGAGG CCCCAGAACA GAAAGGAACT GAGGCAAAAC CAGGACAGAG TAACCCAGTC 
TCTATAGCAG AGACTGCTGA GACGTGTATA GCAGAGCGTA CCCCTTTCAG TTCCAGTAGC 
TCCAGCAAGC ACAGCTCACC TAGCGGGACT GTTGCTGCCA GAGTGACACC TTTTAATTAC 
AACCCTAGCC CTAGGAAGAG CAGCGCAGAC AGCACTTCAG CCCGGCCGTC TCAGATCCCT 
ACGCCAGTGA GCACCAACAC GAAGAAGAGA GATTCGAAGA CTGACAGCAC AGAATCCAGT 
GGAGCCCAAA GTCCTAAACG CCATTCCGGG TCTTACCTCG TGACGTCTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106140.1
CDS	816..9269
Translation	MYAFLRSGPVAALPASVPPVTLGARSSGGGRGRVPGEGRRPGGARACGRGASVWQEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAESPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250280.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250280.1

ORF Insert Sequence:

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGTCCAG CTACACGTGT GGATCACGAA 
ACAGCCAGTG TTTTGAGTTC TAGCGGCACG CACTCTGCTC CTCGAAGGTT GACAAGTCAT 
CTGGGGACAA AGGTGGAAAT GGTGTATTCC TTGTTGTCAA TGCTTGGTAC TCATGATAAG 
GACGATATGT CACGAACTTT GCTAGCTATG TCCAGCTCCC AAGACAGCTG TATATCCATG 
CGGCAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT 
GTATTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC 
CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT 
CATCTTTTGG AACAGATACG AGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCCCAC 
GAACAAGGCA TGGACCAGGA CAAAAACCCA ATGCCAGCTC CTGTTGAGCA TCAGATCTGT 
CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA TTTGATGAAG AGCATAGGCA TGCAATGAAT 
GAACTTGGGG GACTGCAGGC CATTGCAGAG TTATTGCAGG TGGACTGTGA GATGTATGGG 
CTTACTAATG ACCACTACAG TGTTACTTTA AGACGGTATG CTGGAATGGC TTTGACAAAC 
TTGACCTTTG GAGATGTTGC CAACAAGGCT ACGCTGTGTT CTATGAAAGG CTGCATGAGA 
GCACTTGTGG CCCAGTTAAA ATCTGAGAGT GAAGACTTAC AGCAGGTTAT TGCAAGTGTT 
TTGAGGAATT TGTCTTGGCG AGCAGATGTA AATAGCAAAA AGACGTTGAG AGAAGTTGGA 
AGTGTGAAAG CATTGATGGA ATGTGCTTTG GAAGTTAAAA AGGAATCAAC CCTCAAAAGC 
GTTTTGAGTG CCTTATGGAA CCTGTCTGCA CACTGCACTG AGAATAAGGC TGACATCTGT 
GCTGTGGATG GAGCACTGGC ATTTCTGGTT GGCACCCTCA CTTACCGGAG CCAGACAAAT 
ACTTTAGCCA TTATTGAAAG TGGAGGTGGG ATATTACGGA ATGTGTCCAG CTTGATAGCT 
ACAAACGAAG ACCACAGGCA AATCCTAAGA GAGAACAATT GCCTACAAAC TTTATTACAG 
CACTTGAAAT CTCACAGCTT GACAATAGTC AGTAATGCAT GTGGAACTTT GTGGAATCTC 
TCAGCAAGAA ATCCTAAAGA CCAGGAAGCC TTGTGGGACA TGGGGGCAGT GAGCATGCTC 
AAGAACCTCA TTCATTCCAA GCACAAAATG ATTGCCATGG GAAGTGCAGC AGCTTTAAGG 
AATCTCATGG CAAACAGACC TGCAAAGTAT AAGGATGCCA ATATCATGTC TCCCGGCTCA 
AGTCTGCCAT CCCTTCACGT TAGGAAACAG AAAGCTCTAG AAGCTGAGCT AGATGCTCAG 
CATTTATCAG AAACCTTCGA CAACATTGAC AACCTAAGTC CCAAGGCCTC TCACCGGAGT 
AAGCAGAGAC ACAAGCAGAA TCTTTATGGT GACTATGCTT TTGACGCCAA TCGACATGAT 
GATAGTAGGT CAGACAATTT CAATACTGGA AACATGACTG TTCTTTCACC ATATTTAAAT 
ACTACGGTAT TGCCCAGCTC TTCTTCCTCA AGGGGAAGTT TAGACAGTTC TCGTTCTGAG 
AAAGACAGAA GTTTGGAGAG AGAGCGAGGT ATTGGCCTCA GTGCTTACCA TCCAACAACA 
GAAAATGCAG GAACCTCATC AAAACGAGGT CTGCAGATCA CTACCACTGC AGCCCAGATA 
GCCAAAGTTA TGGAAGAAGT ATCAGCCATT CATACCTCCC AGGACGACAG AAGTTCTGCT 
TCTACCACCG AGTTCCATTG TGTGGCAGAC GACAGGAGTG CGGCACGAAG AAGCTCTGCC 
TCCCACACAC ACTCAAACAC ATACAACTTC ACTAAGTCGG AAAATTCAAA TAGGACATGC 
TCTATGCCTT ATGCCAAAGT GGAATATAAA CGATCTTCAA ATGACAGTTT AAATAGTGTC 
ACTAGTAGTG ATGGATATGG TAAAAGAGGC CAAATGAAAC CCTCAGTTGA ATCCTATTCT 
GAAGATGATG AAAGTAAATT TTGCAGTTAT GGTCAGTATC CAGCTGACCT AGCCCATAAG 
ATACACAGTG CAAATCATAT GGATGATAAT GATGGAGAAC TGGATACACC AATAAATTAC 
AGTCTTAAAT ATTCAGATGA GCAGTTGAAC TCAGGAAGGC AGAGTCCCTC ACAGAATGAA 
AGGTGGGCAA GACCAAAGCA TGTGATAGAA GATGAAATAA AGCAAAACGA GCAAAGACAA 
GCAAGAAGCC AGAACACCAG TTATCCTGTC TATTCTGAGA ATACCGATGA CAAACACCTC 
AAATTCCAAC CACATTTTGG ACAACAAGAA TGTGTTTCCC CATATAGGTC AAGGGGAACC 
AGTGGTTCAG AAACAAATCG AATGGGTTCT AGTCATGCAA TTAATCAAAA TGTAAACCAG 
TCTCTGTGTC AGGAAGATGA TTATGAAGAT GATAAACCTA CCAACTACAG TGAACGTTAT 
TCTGAGGAAG AACAACATGA AGAAGAAGAA GAGAGACCGA CAAATTATAG CATAAAATAT 
AATGAAGAGA AACATCATGT GGATCAGCCT ATTGATTATA GTTTAAAATA TGCCACTGAC 
ATTTCTTCCT CACAAAAACC ATCATTTTCA TTCTCAAAGA ATTCATCAGC ACAAAGCACT 
AAACCTGAAC ATCTCTCTCC AAGCAGCGAG AATACAGCTG TACCTCCATC TAATGCCAAA 
AGGCAGAATC AGCTGCGTCC AAGTTCAGCA CAAAGAAATG GCCAGACTCA AAAAGGCACT 
ACTTGCAAAG TCCCCTCCAT CAACCAAGAA ACAATACAGA CTTACTGCGT AGAAGACACC 
CCAATATGTT TTTCAAGGTG CAGTTCATTA TCATCACTGT CATCAGCTGA CGATGAAATA 
GGATGTGATC AGACAACACA GGAAGCAGAT TCTGCTAATA CTCTGCAGAC AGCAGAAGTA 
AAAGAGAATG ATGTAACTCG GTCAGCTGAA GATCCTGCAA CTGAAGTTCC AGCAGTGTCC 
CAGAATGCTA GAGCCAAACC CAGCCGACTC CAGGCTTCTG GCTTATCTTC AGAATCAACC 
AGGCATAATA AAGCTGTTGA GTTTTCTTCA GGAGCCAAGT CTCCCTCCAA AAGTGGTGCT 
CAGACACCCA AAAGTCCCCC AGAACACTAT GTCCAGGAGA CTCCGCTCGT ATTCAGCAGG 
TGTACTTCTG TCAGCTCCCT TGACAGTTTT GAGAGTCGCT CCATTGCCAG CTCTGTTCAG 
AGTGAGCCAT GTAGTGGAAT GGTGAGTGGC ATCATAAGCC CCAGTGACCT TCCAGATAGT 
CCTGGGCAGA CCATGCCACC AAGCAGAAGC AAAACCCCTC CACCTCCTCC ACAGACAGTG 
CAGGCCAAGA GAGAGGTGCC AAAAAGTAAA GTCCCTGCTG CTGAGAAGAG AGAGAGTGGG 
CCTAAGCAGA CTGCTGTAAA TGCTGCCGTG CAGAGGGTGC AGGTCCTTCC AGACGTGGAT 
ACTTTGTTAC ACTTCGCCAC AGAAAGTACT CCAGACGGGT TTTCTTGTTC CTCCAGCCTA 
AGTGCTCTGA GCCTGGATGA GCCATTTATA CAGAAAGATG TAGAATTAAG AATCATGCCT 
CCAGTTCAGG AAAACGACAA TGGGAATGAA ACTGAATCAG AACAGCCTGA GGAATCAAAT 
GAAAACCAGG ATAAAGAGGT AGAAAAGCCT GACTCTGAAA AAGACTTATT AGATGATTCT 
GATGACGATG ATATTGAAAT ATTAGAAGAA TGTATTATTT CAGCCATGCC AACAAAGTCA 
TCACGCAAAG CCAAAAAACT AGCCCAGACT GCTTCAAAAT TACCTCCACC TGTGGCAAGG 
AAACCAAGTC AGCTACCTGT GTATAAACTT CTGCCAGCAC AGAATAGGCT GCAGGCACAA 
AAACATGTTA GCTTTACACC AGGGGATGAT GTGCCCCGGG TGTACTGTGT AGAAGGGACA 
CCTATAAACT TTTCCACAGC AACGTCTCTA AGTGATCTGA CAATAGAGTC CCCTCCAAAT 
GAATTGGCTA CTGGAGATGG GGTCAGAGCG GGTATACAGT CAGGTGAATT TGAAAAACGA 
GATACCATTC CTACAGAAGG CAGAAGTACA GATGATGCTC AGCGAGGAAA AATCTCATCT 
ATAGTTACAC CAGACCTGGA TGACAACAAA GCAGAGGAAG GAGATATTCT TGCAGAATGT 
ATCAATTCTG CTATGCCCAA AGGAAAAAGC CACAAGCCTT TCCGAGTGAA AAAGATAATG 
GACCAAGTCC AACAAGCATC CTCGACTTCA TCTGGAGCTA ACAAAAATCA AGTAGACACT 
AAGAAAAAGA AGCCTACTTC ACCAGTAAAG CCCATGCCAC AAAATACTGA ATATAGAACG 
CGTGTGAGAA AGAATACAGA CTCAAAAGTT AATGTAAATA CTGAAGAAAC TTTCTCAGAC 
AACAAAGACT CAAAGAAACC AAGCTTACAA ACCAATGCCA AGGCCTTCAA TGAAAAGCTA 
CCTAACAATG AAGACAGAGT GCGGGGGAGC TTCGCCTTGG ACTCACCGCA TCACTACACC 
CCTATTGAGG GGACGCCGTA CTGCTTTTCC CGAAATGACT CCTTGAGTTC TCTGGATTTT 
GATGATGACG ATGTTGACCT TTCCAGGGAA AAGGCCGAGT TAAGAAAGGG CAAAGAAAGC 
AAGGATTCCG AAGCCAAAGT TACCTGCCGC CCAGAACCAA ACTCAAGCCA GCAGGCAGCT 
AGTAAGTCAC AAGCCAGTAT AAAACATCCA GCAAACAGAG CACAGTCCAA ACCAGTGCTG 
CAGAAACAGC CCACTTTCCC CCAGTCCTCC AAAGACGGAC CAGATAGAGG GGCAGCAACT 
GACGAAAAAC TGCAGAATTT TGCTATTGAA AATACTCCAG TTTGCTTTTC TCGAAATTCC 
TCTCTGAGTT CCCTTAGTGA CATTGACCAG GAAAACAACA ATAACAAAGA AAGTGAACCA 
ATCAAAGAAG CTGAACCTGC CAACTCACAA GGAGAGCCCA GTAAGCCTCA GGCATCCGGG 
TATGCTCCCA AGTCCTTCCA CGTCGAAGAC ACCCCTGTCT GTTTCTCAAG AAACAGCTCT 
CTCAGTTCTC TTAGCATTGA CTCTGAGGAC GACCTGTTAC AGGAGTGTAT AAGTTCTGCC 
ATGCCAAAAA AGAAAAGGCC TTCAAGACTC AAGAGTGAGA GCGAAAAGCA GAGCCCTAGA 
AAAGTGGGTG GCATATTAGC TGAAGACCTG ACGCTTGATT TGAAAGATCT ACAGAGGCCA 
GATTCAGAAC ACGCTTTCTC CCCCGACTCA GAAAATTTTG ACTGGAAAGC TATTCAGGAA 
GGCGCAAACT CCATAGTAAG TAGTTTGCAC CAAGCTGCTG CAGCCGCCGC GTGCTTATCT 
AGACAAGCGT CATCCGACTC AGATTCCATT CTGTCACTAA AGTCCGGCAT TTCTCTGGGA 
TCGCCTTTTC ATCTTACACC TGATCAAGAG GAAAAGCCAT TCACAAGCAA TAAAGGCCCA 
AGAATTCTCA AACCTGGAGA GAAAAGCACA TTAGAAGCAA AAAAAATAGA ATCTGAAAAC 
AAAGGAATCA AAGGCGGGAA AAAGGTTTAT AAAAGCTTGA TTACGGGAAA GATTCGCTCC 
AATTCAGAAA TTTCCAGCCA AATGAAACAA CCCCTCCCGA CAAACATGCC TTCAATCTCA 
AGAGGCAGGA CGATGATTCA CATCCCAGGG CTTCGGAATA GCTCCTCTAG TACAAGCCCT 
GTCTCTAAGA AAGGCCCACC CCTCAAGACT CCAGCCTCTA AAAGCCCCAG TGAAGGGCCG 
GGAGCTACCA CTTCTCCTCG AGGAACTAAG CCAGCAGGAA AGTCAGAGCT TAGCCCTATC 
ACCAGGCAAA CTTCCCAAAT CAGTGGGTCA AATAAGGGGT CTTCTAGATC AGGATCTAGA 
GACTCCACTC CCTCAAGACC TACACAGCAA CCATTAAGTA GGCCAATGCA GTCTCCAGGG 
CGAAACTCAA TTTCCCCTGG TAGAAATGGA ATAAGCCCTC CTAACAAACT GTCTCAGCTG 
CCCAGAACAT CATCTCCCAG TACTGCTTCA ACTAAGTCCT CCGGTTCTGG GAAAATGTCA 
TATACATCCC CAGGTAGACA GCTGAGCCAA CAAAATCTTA CCAAACAAGC AAGTTTATCC 
AAGAATGCCA GCAGTATCCC CAGAAGTGAG TCGGCATCTA AAGGACTGAA TCAGATGAGT 
AACGGCAATG GGTCAAATAA AAAGGTAGAA CTTTCTAGAA TGTCTTCAAC TAAATCAAGT 
GGAAGTGAAT CAGACAGATC AGAAAGGCCT GCATTAGTAC GCCAGTCTAC TTTCATCAAA 
GAAGCCCCAA GCCCAACCCT GAGGAGGAAA CTGGAGGAAT CTGCCTCATT TGAATCCCTT 
TCTCCATCTT CTAGACCAGA TTCTCCCACC AGGTCGCAGG CACAGACCCC AGTTTTAAGC 
CCTTCCCTTC CTGATATGTC TCTGTCCACA CATCCATCTG TTCAGGCAGG TGGGTGGCGA 
AAGCTCCCGC CTAATCTCAG CCCCACTATC GAGTATAATG ACGGAAGGCC CACAAAACGG 
CATGATATTG CACGCTCCCA TTCTGAAAGT CCTTCCAGAC TACCAATCAA CCGGGCGGGA 
ACCTGGAAGC GTGAACACAG CAAACATTCC TCGTCCCTTC CTCGAGTGAG TACTTGGAGA 
AGAACTGGAA GCTCATCTTC TATTCTTTCT GCTTCATCAG AGTCCAGTGA AAAAGCAAAA 
AGTGAGGATG AAAGGCATGT GAGCTCCATG CCAGCACCCA GACAGATGAA GGAAAACCAG 
GTGCCCACCA AAGGAACATG GAGGAAAATC AAGGAAAGTG ACATTTCTCC CACAGGCATG 
GCTTCTCAGA GCGCTTCCTC AGGTGCTGCC AGTGGTGCTG AATCCAAGCC TCTGATCTAT 
CAGATGGCAC CTCCTGTCTC TAAAACAGAG GATGTTTGGG TGAGAATTGA GGACTGCCCC 
ATTAACAACC CTAGATCTGG ACGGTCCCCC ACAGGCAACA CCCCCCCAGT GATTGACAGT 
GTTTCAGAGA AGGGAAGTTC AAGCATTAAA GATTCAAAAG ACACCCATGG GAAACAGAGT 
GTGGGCAGTG GCAGTCCTGT GCAAACCGTG GGTCTGGAAA CCCGCCTCAA CTCCTTTGTT 
CAGGTAGAGG CCCCAGAACA GAAAGGAACT GAGGCAAAAC CAGGACAGAG TAACCCAGTC 
TCTATAGCAG AGACTGCTGA GACGTGTATA GCAGAGCGTA CCCCTTTCAG TTCCAGTAGC 
TCCAGCAAGC ACAGCTCACC TAGCGGGACT GTTGCTGCCA GAGTGACACC TTTTAATTAC 
AACCCTAGCC CTAGGAAGAG CAGCGCAGAC AGCACTTCAG CCCGGCCGTC TCAGATCCCT 
ACGCCAGTGA GCACCAACAC GAAGAAGAGA GATTCGAAGA CTGACAGCAC AGAATCCAGT 
GGAGCCCAAA GTCCTAAACG CCATTCCGGG TCTTACCTCG TGACGTCTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250281.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106141.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X10, mRNA.

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CloneID	OMu87324
Clone ID Related Accession (Same CDS sequence)	XM_030250281.1
Accession Version	XM_030250281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGTCCAG CTACACGTGT GGATCACGAA ACAGCCAGTG TTTTGAGTTC TAGCGGCACG 
CACTCTGCTC CTCGAAGGTT GACAAGTCAT CTGGGGACAA AGGTGGAAAT GGTGTATTCC 
TTGTTGTCAA TGCTTGGTAC TCATGATAAG GACGATATGT CACGAACTTT GCTAGCTATG 
TCCAGCTCCC AAGACAGCTG TATATCCATG CGGCAGTCTG GATGTCTTCC TCTCCTCATC 
CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTATTGTTGG GAAATTCCCG GGGCAGTAAA 
GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC 
AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT 
GAAACCTGTT GGGAGTGGCA GGAAGCCCAC GAACAAGGCA TGGACCAGGA CAAAAACCCA 
ATGCCAGCTC CTGTTGAGCA TCAGATCTGT CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA 
TTTGATGAAG AGCATAGGCA TGCAATGAAT GAACTTGGGG GACTGCAGGC CATTGCAGAG 
TTATTGCAGG TGGACTGTGA GATGTATGGG CTTACTAATG ACCACTACAG TGTTACTTTA 
AGACGGTATG CTGGAATGGC TTTGACAAAC TTGACCTTTG GAGATGTTGC CAACAAGGCT 
ACGCTGTGTT CTATGAAAGG CTGCATGAGA GCACTTGTGG CCCAGTTAAA ATCTGAGAGT 
GAAGACTTAC AGCAGGTTAT TGCAAGTGTT TTGAGGAATT TGTCTTGGCG AGCAGATGTA 
AATAGCAAAA AGACGTTGAG AGAAGTTGGA AGTGTGAAAG CATTGATGGA ATGTGCTTTG 
GAAGTTAAAA AGGAATCAAC CCTCAAAAGC GTTTTGAGTG CCTTATGGAA CCTGTCTGCA 
CACTGCACTG AGAATAAGGC TGACATCTGT GCTGTGGATG GAGCACTGGC ATTTCTGGTT 
GGCACCCTCA CTTACCGGAG CCAGACAAAT ACTTTAGCCA TTATTGAAAG TGGAGGTGGG 
ATATTACGGA ATGTGTCCAG CTTGATAGCT ACAAACGAAG ACCACAGGCA AATCCTAAGA 
GAGAACAATT GCCTACAAAC TTTATTACAG CACTTGAAAT CTCACAGCTT GACAATAGTC 
AGTAATGCAT GTGGAACTTT GTGGAATCTC TCAGCAAGAA ATCCTAAAGA CCAGGAAGCC 
TTGTGGGACA TGGGGGCAGT GAGCATGCTC AAGAACCTCA TTCATTCCAA GCACAAAATG 
ATTGCCATGG GAAGTGCAGC AGCTTTAAGG AATCTCATGG CAAACAGACC TGCAAAGTAT 
AAGGATGCCA ATATCATGTC TCCCGGCTCA AGTCTGCCAT CCCTTCACGT TAGGAAACAG 
AAAGCTCTAG AAGCTGAGCT AGATGCTCAG CATTTATCAG AAACCTTCGA CAACATTGAC 
AACCTAAGTC CCAAGGCCTC TCACCGGAGT AAGCAGAGAC ACAAGCAGAA TCTTTATGGT 
GACTATGCTT TTGACGCCAA TCGACATGAT GATAGTAGGT CAGACAATTT CAATACTGGA 
AACATGACTG TTCTTTCACC ATATTTAAAT ACTACGGTAT TGCCCAGCTC TTCTTCCTCA 
AGGGGAAGTT TAGACAGTTC TCGTTCTGAG AAAGACAGAA GTTTGGAGAG AGAGCGAGGT 
ATTGGCCTCA GTGCTTACCA TCCAACAACA GAAAATGCAG GAACCTCATC AAAACGAGGT 
CTGCAGATCA CTACCACTGC AGCCCAGATA GCCAAAGTTA TGGAAGAAGT ATCAGCCATT 
CATACCTCCC AGGACGACAG AAGTTCTGCT TCTACCACCG AGTTCCATTG TGTGGCAGAC 
GACAGGAGTG CGGCACGAAG AAGCTCTGCC TCCCACACAC ACTCAAACAC ATACAACTTC 
ACTAAGTCGG AAAATTCAAA TAGGACATGC TCTATGCCTT ATGCCAAAGT GGAATATAAA 
CGATCTTCAA ATGACAGTTT AAATAGTGTC ACTAGTAGTG ATGGATATGG TAAAAGAGGC 
CAAATGAAAC CCTCAGTTGA ATCCTATTCT GAAGATGATG AAAGTAAATT TTGCAGTTAT 
GGTCAGTATC CAGCTGACCT AGCCCATAAG ATACACAGTG CAAATCATAT GGATGATAAT 
GATGGAGAAC TGGATACACC AATAAATTAC AGTCTTAAAT ATTCAGATGA GCAGTTGAAC 
TCAGGAAGGC AGAGTCCCTC ACAGAATGAA AGGTGGGCAA GACCAAAGCA TGTGATAGAA 
GATGAAATAA AGCAAAACGA GCAAAGACAA GCAAGAAGCC AGAACACCAG TTATCCTGTC 
TATTCTGAGA ATACCGATGA CAAACACCTC AAATTCCAAC CACATTTTGG ACAACAAGAA 
TGTGTTTCCC CATATAGGTC AAGGGGAACC AGTGGTTCAG AAACAAATCG AATGGGTTCT 
AGTCATGCAA TTAATCAAAA TGTAAACCAG TCTCTGTGTC AGGAAGATGA TTATGAAGAT 
GATAAACCTA CCAACTACAG TGAACGTTAT TCTGAGGAAG AACAACATGA AGAAGAAGAA 
GAGAGACCGA CAAATTATAG CATAAAATAT AATGAAGAGA AACATCATGT GGATCAGCCT 
ATTGATTATA GTTTAAAATA TGCCACTGAC ATTTCTTCCT CACAAAAACC ATCATTTTCA 
TTCTCAAAGA ATTCATCAGC ACAAAGCACT AAACCTGAAC ATCTCTCTCC AAGCAGCGAG 
AATACAGCTG TACCTCCATC TAATGCCAAA AGGCAGAATC AGCTGCGTCC AAGTTCAGCA 
CAAAGAAATG GCCAGACTCA AAAAGGCACT ACTTGCAAAG TCCCCTCCAT CAACCAAGAA 
ACAATACAGA CTTACTGCGT AGAAGACACC CCAATATGTT TTTCAAGGTG CAGTTCATTA 
TCATCACTGT CATCAGCTGA CGATGAAATA GGATGTGATC AGACAACACA GGAAGCAGAT 
TCTGCTAATA CTCTGCAGAC AGCAGAAGTA AAAGAGAATG ATGTAACTCG GTCAGCTGAA 
GATCCTGCAA CTGAAGTTCC AGCAGTGTCC CAGAATGCTA GAGCCAAACC CAGCCGACTC 
CAGGCTTCTG GCTTATCTTC AGAATCAACC AGGCATAATA AAGCTGTTGA GTTTTCTTCA 
GGAGCCAAGT CTCCCTCCAA AAGTGGTGCT CAGACACCCA AAAGTCCCCC AGAACACTAT 
GTCCAGGAGA CTCCGCTCGT ATTCAGCAGG TGTACTTCTG TCAGCTCCCT TGACAGTTTT 
GAGAGTCGCT CCATTGCCAG CTCTGTTCAG AGTGAGCCAT GTAGTGGAAT GGTGAGTGGC 
ATCATAAGCC CCAGTGACCT TCCAGATAGT CCTGGGCAGA CCATGCCACC AAGCAGAAGC 
AAAACCCCTC CACCTCCTCC ACAGACAGTG CAGGCCAAGA GAGAGGTGCC AAAAAGTAAA 
GTCCCTGCTG CTGAGAAGAG AGAGAGTGGG CCTAAGCAGA CTGCTGTAAA TGCTGCCGTG 
CAGAGGGTGC AGGTCCTTCC AGACGTGGAT ACTTTGTTAC ACTTCGCCAC AGAAAGTACT 
CCAGACGGGT TTTCTTGTTC CTCCAGCCTA AGTGCTCTGA GCCTGGATGA GCCATTTATA 
CAGAAAGATG TAGAATTAAG AATCATGCCT CCAGTTCAGG AAAACGACAA TGGGAATGAA 
ACTGAATCAG AACAGCCTGA GGAATCAAAT GAAAACCAGG ATAAAGAGGT AGAAAAGCCT 
GACTCTGAAA AAGACTTATT AGATGATTCT GATGACGATG ATATTGAAAT ATTAGAAGAA 
TGTATTATTT CAGCCATGCC AACAAAGTCA TCACGCAAAG CCAAAAAACT AGCCCAGACT 
GCTTCAAAAT TACCTCCACC TGTGGCAAGG AAACCAAGTC AGCTACCTGT GTATAAACTT 
CTGCCAGCAC AGAATAGGCT GCAGGCACAA AAACATGTTA GCTTTACACC AGGGGATGAT 
GTGCCCCGGG TGTACTGTGT AGAAGGGACA CCTATAAACT TTTCCACAGC AACGTCTCTA 
AGTGATCTGA CAATAGAGTC CCCTCCAAAT GAATTGGCTA CTGGAGATGG GGTCAGAGCG 
GGTATACAGT CAGGTGAATT TGAAAAACGA GATACCATTC CTACAGAAGG CAGAAGTACA 
GATGATGCTC AGCGAGGAAA AATCTCATCT ATAGTTACAC CAGACCTGGA TGACAACAAA 
GCAGAGGAAG GAGATATTCT TGCAGAATGT ATCAATTCTG CTATGCCCAA AGGAAAAAGC 
CACAAGCCTT TCCGAGTGAA AAAGATAATG GACCAAGTCC AACAAGCATC CTCGACTTCA 
TCTGGAGCTA ACAAAAATCA AGTAGACACT AAGAAAAAGA AGCCTACTTC ACCAGTAAAG 
CCCATGCCAC AAAATACTGA ATATAGAACG CGTGTGAGAA AGAATACAGA CTCAAAAGTT 
AATGTAAATA CTGAAGAAAC TTTCTCAGAC AACAAAGACT CAAAGAAACC AAGCTTACAA 
ACCAATGCCA AGGCCTTCAA TGAAAAGCTA CCTAACAATG AAGACAGAGT GCGGGGGAGC 
TTCGCCTTGG ACTCACCGCA TCACTACACC CCTATTGAGG GGACGCCGTA CTGCTTTTCC 
CGAAATGACT CCTTGAGTTC TCTGGATTTT GATGATGACG ATGTTGACCT TTCCAGGGAA 
AAGGCCGAGT TAAGAAAGGG CAAAGAAAGC AAGGATTCCG AAGCCAAAGT TACCTGCCGC 
CCAGAACCAA ACTCAAGCCA GCAGGCAGCT AGTAAGTCAC AAGCCAGTAT AAAACATCCA 
GCAAACAGAG CACAGTCCAA ACCAGTGCTG CAGAAACAGC CCACTTTCCC CCAGTCCTCC 
AAAGACGGAC CAGATAGAGG GGCAGCAACT GACGAAAAAC TGCAGAATTT TGCTATTGAA 
AATACTCCAG TTTGCTTTTC TCGAAATTCC TCTCTGAGTT CCCTTAGTGA CATTGACCAG 
GAAAACAACA ATAACAAAGA AAGTGAACCA ATCAAAGAAG CTGAACCTGC CAACTCACAA 
GGAGAGCCCA GTAAGCCTCA GGCATCCGGG TATGCTCCCA AGTCCTTCCA CGTCGAAGAC 
ACCCCTGTCT GTTTCTCAAG AAACAGCTCT CTCAGTTCTC TTAGCATTGA CTCTGAGGAC 
GACCTGTTAC AGGAGTGTAT AAGTTCTGCC ATGCCAAAAA AGAAAAGGCC TTCAAGACTC 
AAGAGTGAGA GCGAAAAGCA GAGCCCTAGA AAAGTGGGTG GCATATTAGC TGAAGACCTG 
ACGCTTGATT TGAAAGATCT ACAGAGGCCA GATTCAGAAC ACGCTTTCTC CCCCGACTCA 
GAAAATTTTG ACTGGAAAGC TATTCAGGAA GGCGCAAACT CCATAGTAAG TAGTTTGCAC 
CAAGCTGCTG CAGCCGCCGC GTGCTTATCT AGACAAGCGT CATCCGACTC AGATTCCATT 
CTGTCACTAA AGTCCGGCAT TTCTCTGGGA TCGCCTTTTC ATCTTACACC TGATCAAGAG 
GAAAAGCCAT TCACAAGCAA TAAAGGCCCA AGAATTCTCA AACCTGGAGA GAAAAGCACA 
TTAGAAGCAA AAAAAATAGA ATCTGAAAAC AAAGGAATCA AAGGCGGGAA AAAGGTTTAT 
AAAAGCTTGA TTACGGGAAA GATTCGCTCC AATTCAGAAA TTTCCAGCCA AATGAAACAA 
CCCCTCCCGA CAAACATGCC TTCAATCTCA AGAGGCAGGA CGATGATTCA CATCCCAGGG 
CTTCGGAATA GCTCCTCTAG TACAAGCCCT GTCTCTAAGA AAGGCCCACC CCTCAAGACT 
CCAGCCTCTA AAAGCCCCAG TGAAGGGCCG GGAGCTACCA CTTCTCCTCG AGGAACTAAG 
CCAGCAGGAA AGTCAGAGCT TAGCCCTATC ACCAGGCAAA CTTCCCAAAT CAGTGGGTCA 
AATAAGGGGT CTTCTAGATC AGGATCTAGA GACTCCACTC CCTCAAGACC TACACAGCAA 
CCATTAAGTA GGCCAATGCA GTCTCCAGGG CGAAACTCAA TTTCCCCTGG TAGAAATGGA 
ATAAGCCCTC CTAACAAACT GTCTCAGCTG CCCAGAACAT CATCTCCCAG TACTGCTTCA 
ACTAAGTCCT CCGGTTCTGG GAAAATGTCA TATACATCCC CAGGTAGACA GCTGAGCCAA 
CAAAATCTTA CCAAACAAGC AAGTTTATCC AAGAATGCCA GCAGTATCCC CAGAAGTGAG 
TCGGCATCTA AAGGACTGAA TCAGATGAGT AACGGCAATG GGTCAAATAA AAAGGTAGAA 
CTTTCTAGAA TGTCTTCAAC TAAATCAAGT GGAAGTGAAT CAGACAGATC AGAAAGGCCT 
GCATTAGTAC GCCAGTCTAC TTTCATCAAA GAAGCCCCAA GCCCAACCCT GAGGAGGAAA 
CTGGAGGAAT CTGCCTCATT TGAATCCCTT TCTCCATCTT CTAGACCAGA TTCTCCCACC 
AGGTCGCAGG CACAGACCCC AGTTTTAAGC CCTTCCCTTC CTGATATGTC TCTGTCCACA 
CATCCATCTG TTCAGGCAGG TGGGTGGCGA AAGCTCCCGC CTAATCTCAG CCCCACTATC 
GAGTATAATG ACGGAAGGCC CACAAAACGG CATGATATTG CACGCTCCCA TTCTGAAAGT 
CCTTCCAGAC TACCAATCAA CCGGGCGGGA ACCTGGAAGC GTGAACACAG CAAACATTCC 
TCGTCCCTTC CTCGAGTGAG TACTTGGAGA AGAACTGGAA GCTCATCTTC TATTCTTTCT 
GCTTCATCAG AGTCCAGTGA AAAAGCAAAA AGTGAGGATG AAAGGCATGT GAGCTCCATG 
CCAGCACCCA GACAGATGAA GGAAAACCAG GTGCCCACCA AAGGAACATG GAGGAAAATC 
AAGGAAAGTG ACATTTCTCC CACAGGCATG GCTTCTCAGA GCGCTTCCTC AGGTGCTGCC 
AGTGGTGCTG AATCCAAGCC TCTGATCTAT CAGATGGCAC CTCCTGTCTC TAAAACAGAG 
GATGTTTGGG TGAGAATTGA GGACTGCCCC ATTAACAACC CTAGATCTGG ACGGTCCCCC 
ACAGGCAACA CCCCCCCAGT GATTGACAGT GTTTCAGAGA AGGGAAGTTC AAGCATTAAA 
GATTCAAAAG ACACCCATGG GAAACAGAGT GTGGGCAGTG GCAGTCCTGT GCAAACCGTG 
GGTCTGGAAA CCCGCCTCAA CTCCTTTGTT CAGGTAGAGG CCCCAGAACA GAAAGGAACT 
GAGGCAAAAC CAGGACAGAG TAACCCAGTC TCTATAGCAG AGACTGCTGA GACGTGTATA 
GCAGAGCGTA CCCCTTTCAG TTCCAGTAGC TCCAGCAAGC ACAGCTCACC TAGCGGGACT 
GTTGCTGCCA GAGTGACACC TTTTAATTAC AACCCTAGCC CTAGGAAGAG CAGCGCAGAC 
AGCACTTCAG CCCGGCCGTC TCAGATCCCT ACGCCAGTGA GCACCAACAC GAAGAAGAGA 
GATTCGAAGA CTGACAGCAC AGAATCCAGT GGAGCCCAAA GTCCTAAACG CCATTCCGGG 
TCTTACCTCG TGACGTCTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106141.1
CDS	383..8806
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAESPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250281.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250281.1

ORF Insert Sequence:

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGTCCAG CTACACGTGT GGATCACGAA ACAGCCAGTG TTTTGAGTTC TAGCGGCACG 
CACTCTGCTC CTCGAAGGTT GACAAGTCAT CTGGGGACAA AGGTGGAAAT GGTGTATTCC 
TTGTTGTCAA TGCTTGGTAC TCATGATAAG GACGATATGT CACGAACTTT GCTAGCTATG 
TCCAGCTCCC AAGACAGCTG TATATCCATG CGGCAGTCTG GATGTCTTCC TCTCCTCATC 
CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTATTGTTGG GAAATTCCCG GGGCAGTAAA 
GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC 
AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT 
GAAACCTGTT GGGAGTGGCA GGAAGCCCAC GAACAAGGCA TGGACCAGGA CAAAAACCCA 
ATGCCAGCTC CTGTTGAGCA TCAGATCTGT CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA 
TTTGATGAAG AGCATAGGCA TGCAATGAAT GAACTTGGGG GACTGCAGGC CATTGCAGAG 
TTATTGCAGG TGGACTGTGA GATGTATGGG CTTACTAATG ACCACTACAG TGTTACTTTA 
AGACGGTATG CTGGAATGGC TTTGACAAAC TTGACCTTTG GAGATGTTGC CAACAAGGCT 
ACGCTGTGTT CTATGAAAGG CTGCATGAGA GCACTTGTGG CCCAGTTAAA ATCTGAGAGT 
GAAGACTTAC AGCAGGTTAT TGCAAGTGTT TTGAGGAATT TGTCTTGGCG AGCAGATGTA 
AATAGCAAAA AGACGTTGAG AGAAGTTGGA AGTGTGAAAG CATTGATGGA ATGTGCTTTG 
GAAGTTAAAA AGGAATCAAC CCTCAAAAGC GTTTTGAGTG CCTTATGGAA CCTGTCTGCA 
CACTGCACTG AGAATAAGGC TGACATCTGT GCTGTGGATG GAGCACTGGC ATTTCTGGTT 
GGCACCCTCA CTTACCGGAG CCAGACAAAT ACTTTAGCCA TTATTGAAAG TGGAGGTGGG 
ATATTACGGA ATGTGTCCAG CTTGATAGCT ACAAACGAAG ACCACAGGCA AATCCTAAGA 
GAGAACAATT GCCTACAAAC TTTATTACAG CACTTGAAAT CTCACAGCTT GACAATAGTC 
AGTAATGCAT GTGGAACTTT GTGGAATCTC TCAGCAAGAA ATCCTAAAGA CCAGGAAGCC 
TTGTGGGACA TGGGGGCAGT GAGCATGCTC AAGAACCTCA TTCATTCCAA GCACAAAATG 
ATTGCCATGG GAAGTGCAGC AGCTTTAAGG AATCTCATGG CAAACAGACC TGCAAAGTAT 
AAGGATGCCA ATATCATGTC TCCCGGCTCA AGTCTGCCAT CCCTTCACGT TAGGAAACAG 
AAAGCTCTAG AAGCTGAGCT AGATGCTCAG CATTTATCAG AAACCTTCGA CAACATTGAC 
AACCTAAGTC CCAAGGCCTC TCACCGGAGT AAGCAGAGAC ACAAGCAGAA TCTTTATGGT 
GACTATGCTT TTGACGCCAA TCGACATGAT GATAGTAGGT CAGACAATTT CAATACTGGA 
AACATGACTG TTCTTTCACC ATATTTAAAT ACTACGGTAT TGCCCAGCTC TTCTTCCTCA 
AGGGGAAGTT TAGACAGTTC TCGTTCTGAG AAAGACAGAA GTTTGGAGAG AGAGCGAGGT 
ATTGGCCTCA GTGCTTACCA TCCAACAACA GAAAATGCAG GAACCTCATC AAAACGAGGT 
CTGCAGATCA CTACCACTGC AGCCCAGATA GCCAAAGTTA TGGAAGAAGT ATCAGCCATT 
CATACCTCCC AGGACGACAG AAGTTCTGCT TCTACCACCG AGTTCCATTG TGTGGCAGAC 
GACAGGAGTG CGGCACGAAG AAGCTCTGCC TCCCACACAC ACTCAAACAC ATACAACTTC 
ACTAAGTCGG AAAATTCAAA TAGGACATGC TCTATGCCTT ATGCCAAAGT GGAATATAAA 
CGATCTTCAA ATGACAGTTT AAATAGTGTC ACTAGTAGTG ATGGATATGG TAAAAGAGGC 
CAAATGAAAC CCTCAGTTGA ATCCTATTCT GAAGATGATG AAAGTAAATT TTGCAGTTAT 
GGTCAGTATC CAGCTGACCT AGCCCATAAG ATACACAGTG CAAATCATAT GGATGATAAT 
GATGGAGAAC TGGATACACC AATAAATTAC AGTCTTAAAT ATTCAGATGA GCAGTTGAAC 
TCAGGAAGGC AGAGTCCCTC ACAGAATGAA AGGTGGGCAA GACCAAAGCA TGTGATAGAA 
GATGAAATAA AGCAAAACGA GCAAAGACAA GCAAGAAGCC AGAACACCAG TTATCCTGTC 
TATTCTGAGA ATACCGATGA CAAACACCTC AAATTCCAAC CACATTTTGG ACAACAAGAA 
TGTGTTTCCC CATATAGGTC AAGGGGAACC AGTGGTTCAG AAACAAATCG AATGGGTTCT 
AGTCATGCAA TTAATCAAAA TGTAAACCAG TCTCTGTGTC AGGAAGATGA TTATGAAGAT 
GATAAACCTA CCAACTACAG TGAACGTTAT TCTGAGGAAG AACAACATGA AGAAGAAGAA 
GAGAGACCGA CAAATTATAG CATAAAATAT AATGAAGAGA AACATCATGT GGATCAGCCT 
ATTGATTATA GTTTAAAATA TGCCACTGAC ATTTCTTCCT CACAAAAACC ATCATTTTCA 
TTCTCAAAGA ATTCATCAGC ACAAAGCACT AAACCTGAAC ATCTCTCTCC AAGCAGCGAG 
AATACAGCTG TACCTCCATC TAATGCCAAA AGGCAGAATC AGCTGCGTCC AAGTTCAGCA 
CAAAGAAATG GCCAGACTCA AAAAGGCACT ACTTGCAAAG TCCCCTCCAT CAACCAAGAA 
ACAATACAGA CTTACTGCGT AGAAGACACC CCAATATGTT TTTCAAGGTG CAGTTCATTA 
TCATCACTGT CATCAGCTGA CGATGAAATA GGATGTGATC AGACAACACA GGAAGCAGAT 
TCTGCTAATA CTCTGCAGAC AGCAGAAGTA AAAGAGAATG ATGTAACTCG GTCAGCTGAA 
GATCCTGCAA CTGAAGTTCC AGCAGTGTCC CAGAATGCTA GAGCCAAACC CAGCCGACTC 
CAGGCTTCTG GCTTATCTTC AGAATCAACC AGGCATAATA AAGCTGTTGA GTTTTCTTCA 
GGAGCCAAGT CTCCCTCCAA AAGTGGTGCT CAGACACCCA AAAGTCCCCC AGAACACTAT 
GTCCAGGAGA CTCCGCTCGT ATTCAGCAGG TGTACTTCTG TCAGCTCCCT TGACAGTTTT 
GAGAGTCGCT CCATTGCCAG CTCTGTTCAG AGTGAGCCAT GTAGTGGAAT GGTGAGTGGC 
ATCATAAGCC CCAGTGACCT TCCAGATAGT CCTGGGCAGA CCATGCCACC AAGCAGAAGC 
AAAACCCCTC CACCTCCTCC ACAGACAGTG CAGGCCAAGA GAGAGGTGCC AAAAAGTAAA 
GTCCCTGCTG CTGAGAAGAG AGAGAGTGGG CCTAAGCAGA CTGCTGTAAA TGCTGCCGTG 
CAGAGGGTGC AGGTCCTTCC AGACGTGGAT ACTTTGTTAC ACTTCGCCAC AGAAAGTACT 
CCAGACGGGT TTTCTTGTTC CTCCAGCCTA AGTGCTCTGA GCCTGGATGA GCCATTTATA 
CAGAAAGATG TAGAATTAAG AATCATGCCT CCAGTTCAGG AAAACGACAA TGGGAATGAA 
ACTGAATCAG AACAGCCTGA GGAATCAAAT GAAAACCAGG ATAAAGAGGT AGAAAAGCCT 
GACTCTGAAA AAGACTTATT AGATGATTCT GATGACGATG ATATTGAAAT ATTAGAAGAA 
TGTATTATTT CAGCCATGCC AACAAAGTCA TCACGCAAAG CCAAAAAACT AGCCCAGACT 
GCTTCAAAAT TACCTCCACC TGTGGCAAGG AAACCAAGTC AGCTACCTGT GTATAAACTT 
CTGCCAGCAC AGAATAGGCT GCAGGCACAA AAACATGTTA GCTTTACACC AGGGGATGAT 
GTGCCCCGGG TGTACTGTGT AGAAGGGACA CCTATAAACT TTTCCACAGC AACGTCTCTA 
AGTGATCTGA CAATAGAGTC CCCTCCAAAT GAATTGGCTA CTGGAGATGG GGTCAGAGCG 
GGTATACAGT CAGGTGAATT TGAAAAACGA GATACCATTC CTACAGAAGG CAGAAGTACA 
GATGATGCTC AGCGAGGAAA AATCTCATCT ATAGTTACAC CAGACCTGGA TGACAACAAA 
GCAGAGGAAG GAGATATTCT TGCAGAATGT ATCAATTCTG CTATGCCCAA AGGAAAAAGC 
CACAAGCCTT TCCGAGTGAA AAAGATAATG GACCAAGTCC AACAAGCATC CTCGACTTCA 
TCTGGAGCTA ACAAAAATCA AGTAGACACT AAGAAAAAGA AGCCTACTTC ACCAGTAAAG 
CCCATGCCAC AAAATACTGA ATATAGAACG CGTGTGAGAA AGAATACAGA CTCAAAAGTT 
AATGTAAATA CTGAAGAAAC TTTCTCAGAC AACAAAGACT CAAAGAAACC AAGCTTACAA 
ACCAATGCCA AGGCCTTCAA TGAAAAGCTA CCTAACAATG AAGACAGAGT GCGGGGGAGC 
TTCGCCTTGG ACTCACCGCA TCACTACACC CCTATTGAGG GGACGCCGTA CTGCTTTTCC 
CGAAATGACT CCTTGAGTTC TCTGGATTTT GATGATGACG ATGTTGACCT TTCCAGGGAA 
AAGGCCGAGT TAAGAAAGGG CAAAGAAAGC AAGGATTCCG AAGCCAAAGT TACCTGCCGC 
CCAGAACCAA ACTCAAGCCA GCAGGCAGCT AGTAAGTCAC AAGCCAGTAT AAAACATCCA 
GCAAACAGAG CACAGTCCAA ACCAGTGCTG CAGAAACAGC CCACTTTCCC CCAGTCCTCC 
AAAGACGGAC CAGATAGAGG GGCAGCAACT GACGAAAAAC TGCAGAATTT TGCTATTGAA 
AATACTCCAG TTTGCTTTTC TCGAAATTCC TCTCTGAGTT CCCTTAGTGA CATTGACCAG 
GAAAACAACA ATAACAAAGA AAGTGAACCA ATCAAAGAAG CTGAACCTGC CAACTCACAA 
GGAGAGCCCA GTAAGCCTCA GGCATCCGGG TATGCTCCCA AGTCCTTCCA CGTCGAAGAC 
ACCCCTGTCT GTTTCTCAAG AAACAGCTCT CTCAGTTCTC TTAGCATTGA CTCTGAGGAC 
GACCTGTTAC AGGAGTGTAT AAGTTCTGCC ATGCCAAAAA AGAAAAGGCC TTCAAGACTC 
AAGAGTGAGA GCGAAAAGCA GAGCCCTAGA AAAGTGGGTG GCATATTAGC TGAAGACCTG 
ACGCTTGATT TGAAAGATCT ACAGAGGCCA GATTCAGAAC ACGCTTTCTC CCCCGACTCA 
GAAAATTTTG ACTGGAAAGC TATTCAGGAA GGCGCAAACT CCATAGTAAG TAGTTTGCAC 
CAAGCTGCTG CAGCCGCCGC GTGCTTATCT AGACAAGCGT CATCCGACTC AGATTCCATT 
CTGTCACTAA AGTCCGGCAT TTCTCTGGGA TCGCCTTTTC ATCTTACACC TGATCAAGAG 
GAAAAGCCAT TCACAAGCAA TAAAGGCCCA AGAATTCTCA AACCTGGAGA GAAAAGCACA 
TTAGAAGCAA AAAAAATAGA ATCTGAAAAC AAAGGAATCA AAGGCGGGAA AAAGGTTTAT 
AAAAGCTTGA TTACGGGAAA GATTCGCTCC AATTCAGAAA TTTCCAGCCA AATGAAACAA 
CCCCTCCCGA CAAACATGCC TTCAATCTCA AGAGGCAGGA CGATGATTCA CATCCCAGGG 
CTTCGGAATA GCTCCTCTAG TACAAGCCCT GTCTCTAAGA AAGGCCCACC CCTCAAGACT 
CCAGCCTCTA AAAGCCCCAG TGAAGGGCCG GGAGCTACCA CTTCTCCTCG AGGAACTAAG 
CCAGCAGGAA AGTCAGAGCT TAGCCCTATC ACCAGGCAAA CTTCCCAAAT CAGTGGGTCA 
AATAAGGGGT CTTCTAGATC AGGATCTAGA GACTCCACTC CCTCAAGACC TACACAGCAA 
CCATTAAGTA GGCCAATGCA GTCTCCAGGG CGAAACTCAA TTTCCCCTGG TAGAAATGGA 
ATAAGCCCTC CTAACAAACT GTCTCAGCTG CCCAGAACAT CATCTCCCAG TACTGCTTCA 
ACTAAGTCCT CCGGTTCTGG GAAAATGTCA TATACATCCC CAGGTAGACA GCTGAGCCAA 
CAAAATCTTA CCAAACAAGC AAGTTTATCC AAGAATGCCA GCAGTATCCC CAGAAGTGAG 
TCGGCATCTA AAGGACTGAA TCAGATGAGT AACGGCAATG GGTCAAATAA AAAGGTAGAA 
CTTTCTAGAA TGTCTTCAAC TAAATCAAGT GGAAGTGAAT CAGACAGATC AGAAAGGCCT 
GCATTAGTAC GCCAGTCTAC TTTCATCAAA GAAGCCCCAA GCCCAACCCT GAGGAGGAAA 
CTGGAGGAAT CTGCCTCATT TGAATCCCTT TCTCCATCTT CTAGACCAGA TTCTCCCACC 
AGGTCGCAGG CACAGACCCC AGTTTTAAGC CCTTCCCTTC CTGATATGTC TCTGTCCACA 
CATCCATCTG TTCAGGCAGG TGGGTGGCGA AAGCTCCCGC CTAATCTCAG CCCCACTATC 
GAGTATAATG ACGGAAGGCC CACAAAACGG CATGATATTG CACGCTCCCA TTCTGAAAGT 
CCTTCCAGAC TACCAATCAA CCGGGCGGGA ACCTGGAAGC GTGAACACAG CAAACATTCC 
TCGTCCCTTC CTCGAGTGAG TACTTGGAGA AGAACTGGAA GCTCATCTTC TATTCTTTCT 
GCTTCATCAG AGTCCAGTGA AAAAGCAAAA AGTGAGGATG AAAGGCATGT GAGCTCCATG 
CCAGCACCCA GACAGATGAA GGAAAACCAG GTGCCCACCA AAGGAACATG GAGGAAAATC 
AAGGAAAGTG ACATTTCTCC CACAGGCATG GCTTCTCAGA GCGCTTCCTC AGGTGCTGCC 
AGTGGTGCTG AATCCAAGCC TCTGATCTAT CAGATGGCAC CTCCTGTCTC TAAAACAGAG 
GATGTTTGGG TGAGAATTGA GGACTGCCCC ATTAACAACC CTAGATCTGG ACGGTCCCCC 
ACAGGCAACA CCCCCCCAGT GATTGACAGT GTTTCAGAGA AGGGAAGTTC AAGCATTAAA 
GATTCAAAAG ACACCCATGG GAAACAGAGT GTGGGCAGTG GCAGTCCTGT GCAAACCGTG 
GGTCTGGAAA CCCGCCTCAA CTCCTTTGTT CAGGTAGAGG CCCCAGAACA GAAAGGAACT 
GAGGCAAAAC CAGGACAGAG TAACCCAGTC TCTATAGCAG AGACTGCTGA GACGTGTATA 
GCAGAGCGTA CCCCTTTCAG TTCCAGTAGC TCCAGCAAGC ACAGCTCACC TAGCGGGACT 
GTTGCTGCCA GAGTGACACC TTTTAATTAC AACCCTAGCC CTAGGAAGAG CAGCGCAGAC 
AGCACTTCAG CCCGGCCGTC TCAGATCCCT ACGCCAGTGA GCACCAACAC GAAGAAGAGA 
GATTCGAAGA CTGACAGCAC AGAATCCAGT GGAGCCCAAA GTCCTAAACG CCATTCCGGG 
TCTTACCTCG TGACGTCTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250282.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106142.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X11, mRNA.

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CloneID	OMu87325
Clone ID Related Accession (Same CDS sequence)	XM_030250282.1
Accession Version	XM_030250282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGT ATTGGCGCAG CTTGCCGGGG TGTCACTGGA GACAGAATGG AGGTGCTGCC 
GGGCTCGAAA ATGGGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGTCCAG CTACACGTGT GGATCACGAA ACAGCCAGTG TTTTGAGTTC TAGCGGCACG 
CACTCTGCTC CTCGAAGGTT GACAAGTCAT CTGGGGACAA AGGTGGAAAT GGTGTATTCC 
TTGTTGTCAA TGCTTGGTAC TCATGATAAG GACGATATGT CACGAACTTT GCTAGCTATG 
TCCAGCTCCC AAGACAGCTG TATATCCATG CGGCAGTCTG GATGTCTTCC TCTCCTCATC 
CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTATTGTTGG GAAATTCCCG GGGCAGTAAA 
GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC 
AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT 
GAAACCTGTT GGGAGTGGCA GGAAGCCCAC GAACAAGGCA TGGACCAGGA CAAAAACCCA 
ATGCCAGCTC CTGTTGAGCA TCAGATCTGT CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA 
TTTGATGAAG AGCATAGGCA TGCAATGAAT GAACTTGGTA GGAAGGCTAC CCGGGGCATT 
TCATCCCAGG AGCTAGGTCA GGGGCTTTCA GGGGGACTGC AGGCCATTGC AGAGTTATTG 
CAGGTGGACT GTGAGATGTA TGGGCTTACT AATGACCACT ACAGTGTTAC TTTAAGACGG 
TATGCTGGAA TGGCTTTGAC AAACTTGACC TTTGGAGATG TTGCCAACAA GGCTACGCTG 
TGTTCTATGA AAGGCTGCAT GAGAGCACTT GTGGCCCAGT TAAAATCTGA GAGTGAAGAC 
TTACAGCAGG TTATTGCAAG TGTTTTGAGG AATTTGTCTT GGCGAGCAGA TGTAAATAGC 
AAAAAGACGT TGAGAGAAGT TGGAAGTGTG AAAGCATTGA TGGAATGTGC TTTGGAAGTT 
AAAAAGGAAT CAACCCTCAA AAGCGTTTTG AGTGCCTTAT GGAACCTGTC TGCACACTGC 
ACTGAGAATA AGGCTGACAT CTGTGCTGTG GATGGAGCAC TGGCATTTCT GGTTGGCACC 
CTCACTTACC GGAGCCAGAC AAATACTTTA GCCATTATTG AAAGTGGAGG TGGGATATTA 
CGGAATGTGT CCAGCTTGAT AGCTACAAAC GAAGACCACA GGCAAATCCT AAGAGAGAAC 
AATTGCCTAC AAACTTTATT ACAGCACTTG AAATCTCACA GCTTGACAAT AGTCAGTAAT 
GCATGTGGAA CTTTGTGGAA TCTCTCAGCA AGAAATCCTA AAGACCAGGA AGCCTTGTGG 
GACATGGGGG CAGTGAGCAT GCTCAAGAAC CTCATTCATT CCAAGCACAA AATGATTGCC 
ATGGGAAGTG CAGCAGCTTT AAGGAATCTC ATGGCAAACA GACCTGCAAA GTATAAGGAT 
GCCAATATCA TGTCTCCCGG CTCAAGTCTG CCATCCCTTC ACGTTAGGAA ACAGAAAGCT 
CTAGAAGCTG AGCTAGATGC TCAGCATTTA TCAGAAACCT TCGACAACAT TGACAACCTA 
AGTCCCAAGG CCTCTCACCG GAGTAAGCAG AGACACAAGC AGAATCTTTA TGGTGACTAT 
GCTTTTGACG CCAATCGACA TGATGATAGT AGGTCAGACA ATTTCAATAC TGGAAACATG 
ACTGTTCTTT CACCATATTT AAATACTACG GTATTGCCCA GCTCTTCTTC CTCAAGGGGA 
AGTTTAGACA GTTCTCGTTC TGAGAAAGAC AGAAGTTTGG AGAGAGAGCG AGGTATTGGC 
CTCAGTGCTT ACCATCCAAC AACAGAAAAT GCAGGAACCT CATCAAAACG AGGTCTGCAG 
ATCACTACCA CTGCAGCCCA GATAGCCAAA GTTATGGAAG AAGTATCAGC CATTCATACC 
TCCCAGGACG ACAGAAGTTC TGCTTCTACC ACCGAGTTCC ATTGTGTGGC AGACGACAGG 
AGTGCGGCAC GAAGAAGCTC TGCCTCCCAC ACACACTCAA ACACATACAA CTTCACTAAG 
TCGGAAAATT CAAATAGGAC ATGCTCTATG CCTTATGCCA AAGTGGAATA TAAACGATCT 
TCAAATGACA GTTTAAATAG TGTCACTAGT AGTGATGGAT ATGGTAAAAG AGGCCAAATG 
AAACCCTCAG TTGAATCCTA TTCTGAAGAT GATGAAAGTA AATTTTGCAG TTATGGTCAG 
TATCCAGCTG ACCTAGCCCA TAAGATACAC AGTGCAAATC ATATGGATGA TAATGATGGA 
GAACTGGATA CACCAATAAA TTACAGTCTT AAATATTCAG ATGAGCAGTT GAACTCAGGA 
AGGCAGAGTC CCTCACAGAA TGAAAGGTGG GCAAGACCAA AGCATGTGAT AGAAGATGAA 
ATAAAGCAAA ACGAGCAAAG ACAAGCAAGA AGCCAGAACA CCAGTTATCC TGTCTATTCT 
GAGAATACCG ATGACAAACA CCTCAAATTC CAACCACATT TTGGACAACA AGAATGTGTT 
TCCCCATATA GGTCAAGGGG AACCAGTGGT TCAGAAACAA ATCGAATGGG TTCTAGTCAT 
GCAATTAATC AAAATGTAAA CCAGTCTCTG TGTCAGGAAG ATGATTATGA AGATGATAAA 
CCTACCAACT ACAGTGAACG TTATTCTGAG GAAGAACAAC ATGAAGAAGA AGAAGAGAGA 
CCGACAAATT ATAGCATAAA ATATAATGAA GAGAAACATC ATGTGGATCA GCCTATTGAT 
TATAGTTTAA AATATGCCAC TGACATTTCT TCCTCACAAA AACCATCATT TTCATTCTCA 
AAGAATTCAT CAGCACAAAG CACTAAACCT GAACATCTCT CTCCAAGCAG CGAGAATACA 
GCTGTACCTC CATCTAATGC CAAAAGGCAG AATCAGCTGC GTCCAAGTTC AGCACAAAGA 
AATGGCCAGA CTCAAAAAGG CACTACTTGC AAAGTCCCCT CCATCAACCA AGAAACAATA 
CAGACTTACT GCGTAGAAGA CACCCCAATA TGTTTTTCAA GGTGCAGTTC ATTATCATCA 
CTGTCATCAG CTGACGATGA AATAGGATGT GATCAGACAA CACAGGAAGC AGATTCTGCT 
AATACTCTGC AGACAGCAGA AGTAAAAGAG AATGATGTAA CTCGGTCAGC TGAAGATCCT 
GCAACTGAAG TTCCAGCAGT GTCCCAGAAT GCTAGAGCCA AACCCAGCCG ACTCCAGGCT 
TCTGGCTTAT CTTCAGAATC AACCAGGCAT AATAAAGCTG TTGAGTTTTC TTCAGGAGCC 
AAGTCTCCCT CCAAAAGTGG TGCTCAGACA CCCAAAAGTC CCCCAGAACA CTATGTCCAG 
GAGACTCCGC TCGTATTCAG CAGGTGTACT TCTGTCAGCT CCCTTGACAG TTTTGAGAGT 
CGCTCCATTG CCAGCTCTGT TCAGAGTGAG CCATGTAGTG GAATGGTGAG TGGCATCATA 
AGCCCCAGTG ACCTTCCAGA TAGTCCTGGG CAGACCATGC CACCAAGCAG AAGCAAAACC 
CCTCCACCTC CTCCACAGAC AGTGCAGGCC AAGAGAGAGG TGCCAAAAAG TAAAGTCCCT 
GCTGCTGAGA AGAGAGAGAG TGGGCCTAAG CAGACTGCTG TAAATGCTGC CGTGCAGAGG 
GTGCAGGTCC TTCCAGACGT GGATACTTTG TTACACTTCG CCACAGAAAG TACTCCAGAC 
GGGTTTTCTT GTTCCTCCAG CCTAAGTGCT CTGAGCCTGG ATGAGCCATT TATACAGAAA 
GATGTAGAAT TAAGAATCAT GCCTCCAGTT CAGGAAAACG ACAATGGGAA TGAAACTGAA 
TCAGAACAGC CTGAGGAATC AAATGAAAAC CAGGATAAAG AGGTAGAAAA GCCTGACTCT 
GAAAAAGACT TATTAGATGA TTCTGATGAC GATGATATTG AAATATTAGA AGAATGTATT 
ATTTCAGCCA TGCCAACAAA GTCATCACGC AAAGCCAAAA AACTAGCCCA GACTGCTTCA 
AAATTACCTC CACCTGTGGC AAGGAAACCA AGTCAGCTAC CTGTGTATAA ACTTCTGCCA 
GCACAGAATA GGCTGCAGGC ACAAAAACAT GTTAGCTTTA CACCAGGGGA TGATGTGCCC 
CGGGTGTACT GTGTAGAAGG GACACCTATA AACTTTTCCA CAGCAACGTC TCTAAGTGAT 
CTGACAATAG AGTCCCCTCC AAATGAATTG GCTACTGGAG ATGGGGTCAG AGCGGGTATA 
CAGTCAGGTG AATTTGAAAA ACGAGATACC ATTCCTACAG AAGGCAGAAG TACAGATGAT 
GCTCAGCGAG GAAAAATCTC ATCTATAGTT ACACCAGACC TGGATGACAA CAAAGCAGAG 
GAAGGAGATA TTCTTGCAGA ATGTATCAAT TCTGCTATGC CCAAAGGAAA AAGCCACAAG 
CCTTTCCGAG TGAAAAAGAT AATGGACCAA GTCCAACAAG CATCCTCGAC TTCATCTGGA 
GCTAACAAAA ATCAAGTAGA CACTAAGAAA AAGAAGCCTA CTTCACCAGT AAAGCCCATG 
CCACAAAATA CTGAATATAG AACGCGTGTG AGAAAGAATA CAGACTCAAA AGTTAATGTA 
AATACTGAAG AAACTTTCTC AGACAACAAA GACTCAAAGA AACCAAGCTT ACAAACCAAT 
GCCAAGGCCT TCAATGAAAA GCTACCTAAC AATGAAGACA GAGTGCGGGG GAGCTTCGCC 
TTGGACTCAC CGCATCACTA CACCCCTATT GAGGGGACGC CGTACTGCTT TTCCCGAAAT 
GACTCCTTGA GTTCTCTGGA TTTTGATGAT GACGATGTTG ACCTTTCCAG GGAAAAGGCC 
GAGTTAAGAA AGGGCAAAGA AAGCAAGGAT TCCGAAGCCA AAGTTACCTG CCGCCCAGAA 
CCAAACTCAA GCCAGCAGGC AGCTAGTAAG TCACAAGCCA GTATAAAACA TCCAGCAAAC 
AGAGCACAGT CCAAACCAGT GCTGCAGAAA CAGCCCACTT TCCCCCAGTC CTCCAAAGAC 
GGACCAGATA GAGGGGCAGC AACTGACGAA AAACTGCAGA ATTTTGCTAT TGAAAATACT 
CCAGTTTGCT TTTCTCGAAA TTCCTCTCTG AGTTCCCTTA GTGACATTGA CCAGGAAAAC 
AACAATAACA AAGAAAGTGA ACCAATCAAA GAAGCTGAAC CTGCCAACTC ACAAGGAGAG 
CCCAGTAAGC CTCAGGCATC CGGGTATGCT CCCAAGTCCT TCCACGTCGA AGACACCCCT 
GTCTGTTTCT CAAGAAACAG CTCTCTCAGT TCTCTTAGCA TTGACTCTGA GGACGACCTG 
TTACAGGAGT GTATAAGTTC TGCCATGCCA AAAAAGAAAA GGCCTTCAAG ACTCAAGAGT 
GAGAGCGAAA AGCAGAGCCC TAGAAAAGTG GGTGGCATAT TAGCTGAAGA CCTGACGCTT 
GATTTGAAAG ATCTACAGAG GCCAGATTCA GAACACGCTT TCTCCCCCGA CTCAGAAAAT 
TTTGACTGGA AAGCTATTCA GGAAGGCGCA AACTCCATAG TAAGTAGTTT GCACCAAGCT 
GCTGCAGCCG CCGCGTGCTT ATCTAGACAA GCGTCATCCG ACTCAGATTC CATTCTGTCA 
CTAAAGTCCG GCATTTCTCT GGGATCGCCT TTTCATCTTA CACCTGATCA AGAGGAAAAG 
CCATTCACAA GCAATAAAGG CCCAAGAATT CTCAAACCTG GAGAGAAAAG CACATTAGAA 
GCAAAAAAAA TAGAATCTGA AAACAAAGGA ATCAAAGGCG GGAAAAAGGT TTATAAAAGC 
TTGATTACGG GAAAGATTCG CTCCAATTCA GAAATTTCCA GCCAAATGAA ACAACCCCTC 
CCGACAAACA TGCCTTCAAT CTCAAGAGGC AGGACGATGA TTCACATCCC AGGGCTTCGG 
AATAGCTCCT CTAGTACAAG CCCTGTCTCT AAGAAAGGCC CACCCCTCAA GACTCCAGCC 
TCTAAAAGCC CCAGTGAAGG GCCGGGAGCT ACCACTTCTC CTCGAGGAAC TAAGCCAGCA 
GGAAAGTCAG AGCTTAGCCC TATCACCAGG CAAACTTCCC AAATCAGTGG GTCAAATAAG 
GGGTCTTCTA GATCAGGATC TAGAGACTCC ACTCCCTCAA GACCTACACA GCAACCATTA 
AGTAGGCCAA TGCAGTCTCC AGGGCGAAAC TCAATTTCCC CTGGTAGAAA TGGAATAAGC 
CCTCCTAACA AACTGTCTCA GCTGCCCAGA ACATCATCTC CCAGTACTGC TTCAACTAAG 
TCCTCCGGTT CTGGGAAAAT GTCATATACA TCCCCAGGTA GACAGCTGAG CCAACAAAAT 
CTTACCAAAC AAGCAAGTTT ATCCAAGAAT GCCAGCAGTA TCCCCAGAAG TGAGTCGGCA 
TCTAAAGGAC TGAATCAGAT GAGTAACGGC AATGGGTCAA ATAAAAAGGT AGAACTTTCT 
AGAATGTCTT CAACTAAATC AAGTGGAAGT GAATCAGACA GATCAGAAAG GCCTGCATTA 
GTACGCCAGT CTACTTTCAT CAAAGAAGCC CCAAGCCCAA CCCTGAGGAG GAAACTGGAG 
GAATCTGCCT CATTTGAATC CCTTTCTCCA TCTTCTAGAC CAGATTCTCC CACCAGGTCG 
CAGGCACAGA CCCCAGTTTT AAGCCCTTCC CTTCCTGATA TGTCTCTGTC CACACATCCA 
TCTGTTCAGG CAGGTGGGTG GCGAAAGCTC CCGCCTAATC TCAGCCCCAC TATCGAGTAT 
AATGACGGAA GGCCCACAAA ACGGCATGAT ATTGCACGCT CCCATTCTGA AAGTCCTTCC 
AGACTACCAA TCAACCGGGC GGGAACCTGG AAGCGTGAAC ACAGCAAACA TTCCTCGTCC 
CTTCCTCGAG TGAGTACTTG GAGAAGAACT GGAAGCTCAT CTTCTATTCT TTCTGCTTCA 
TCAGAGTCCA GTGAAAAAGC AAAAAGTGAG GATGAAAGGC ATGTGAGCTC CATGCCAGCA 
CCCAGACAGA TGAAGGAAAA CCAGGTGCCC ACCAAAGGAA CATGGAGGAA AATCAAGGAA 
AGTGACATTT CTCCCACAGG CATGGCTTCT CAGAGCGCTT CCTCAGGTGC TGCCAGTGGT 
GCTGAATCCA AGCCTCTGAT CTATCAGATG GCACCTCCTG TCTCTAAAAC AGAGGATGTT 
TGGGTGAGAA TTGAGGACTG CCCCATTAAC AACCCTAGAT CTGGACGGTC CCCCACAGGC 
AACACCCCCC CAGTGATTGA CAGTGTTTCA GAGAAGGGAA GTTCAAGCAT TAAAGATTCA 
AAAGACACCC ATGGGAAACA GAGTGTGGGC AGTGGCAGTC CTGTGCAAAC CGTGGGTCTG 
GAAACCCGCC TCAACTCCTT TGTTCAGGTA GAGGCCCCAG AACAGAAAGG AACTGAGGCA 
AAACCAGGAC AGAGTAACCC AGTCTCTATA GCAGAGACTG CTGAGACGTG TATAGCAGAG 
CGTACCCCTT TCAGTTCCAG TAGCTCCAGC AAGCACAGCT CACCTAGCGG GACTGTTGCT 
GCCAGAGTGA CACCTTTTAA TTACAACCCT AGCCCTAGGA AGAGCAGCGC AGACAGCACT 
TCAGCCCGGC CGTCTCAGAT CCCTACGCCA GTGAGCACCA ACACGAAGAA GAGAGATTCG 
AAGACTGACA GCACAGAATC CAGTGGAGCC CAAAGTCCTA AACGCCATTC CGGGTCTTAC 
CTCGTGACGT CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106142.1
CDS	133..8550
Translation	MWLYWRSLPGCHWRQNGGAAGLENGEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAESPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250282.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250282.1

ORF Insert Sequence:

ATGTGGCTGT ATTGGCGCAG CTTGCCGGGG TGTCACTGGA GACAGAATGG AGGTGCTGCC 
GGGCTCGAAA ATGGGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGTCCAG CTACACGTGT GGATCACGAA ACAGCCAGTG TTTTGAGTTC TAGCGGCACG 
CACTCTGCTC CTCGAAGGTT GACAAGTCAT CTGGGGACAA AGGTGGAAAT GGTGTATTCC 
TTGTTGTCAA TGCTTGGTAC TCATGATAAG GACGATATGT CACGAACTTT GCTAGCTATG 
TCCAGCTCCC AAGACAGCTG TATATCCATG CGGCAGTCTG GATGTCTTCC TCTCCTCATC 
CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTATTGTTGG GAAATTCCCG GGGCAGTAAA 
GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC 
AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT 
GAAACCTGTT GGGAGTGGCA GGAAGCCCAC GAACAAGGCA TGGACCAGGA CAAAAACCCA 
ATGCCAGCTC CTGTTGAGCA TCAGATCTGT CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA 
TTTGATGAAG AGCATAGGCA TGCAATGAAT GAACTTGGTA GGAAGGCTAC CCGGGGCATT 
TCATCCCAGG AGCTAGGTCA GGGGCTTTCA GGGGGACTGC AGGCCATTGC AGAGTTATTG 
CAGGTGGACT GTGAGATGTA TGGGCTTACT AATGACCACT ACAGTGTTAC TTTAAGACGG 
TATGCTGGAA TGGCTTTGAC AAACTTGACC TTTGGAGATG TTGCCAACAA GGCTACGCTG 
TGTTCTATGA AAGGCTGCAT GAGAGCACTT GTGGCCCAGT TAAAATCTGA GAGTGAAGAC 
TTACAGCAGG TTATTGCAAG TGTTTTGAGG AATTTGTCTT GGCGAGCAGA TGTAAATAGC 
AAAAAGACGT TGAGAGAAGT TGGAAGTGTG AAAGCATTGA TGGAATGTGC TTTGGAAGTT 
AAAAAGGAAT CAACCCTCAA AAGCGTTTTG AGTGCCTTAT GGAACCTGTC TGCACACTGC 
ACTGAGAATA AGGCTGACAT CTGTGCTGTG GATGGAGCAC TGGCATTTCT GGTTGGCACC 
CTCACTTACC GGAGCCAGAC AAATACTTTA GCCATTATTG AAAGTGGAGG TGGGATATTA 
CGGAATGTGT CCAGCTTGAT AGCTACAAAC GAAGACCACA GGCAAATCCT AAGAGAGAAC 
AATTGCCTAC AAACTTTATT ACAGCACTTG AAATCTCACA GCTTGACAAT AGTCAGTAAT 
GCATGTGGAA CTTTGTGGAA TCTCTCAGCA AGAAATCCTA AAGACCAGGA AGCCTTGTGG 
GACATGGGGG CAGTGAGCAT GCTCAAGAAC CTCATTCATT CCAAGCACAA AATGATTGCC 
ATGGGAAGTG CAGCAGCTTT AAGGAATCTC ATGGCAAACA GACCTGCAAA GTATAAGGAT 
GCCAATATCA TGTCTCCCGG CTCAAGTCTG CCATCCCTTC ACGTTAGGAA ACAGAAAGCT 
CTAGAAGCTG AGCTAGATGC TCAGCATTTA TCAGAAACCT TCGACAACAT TGACAACCTA 
AGTCCCAAGG CCTCTCACCG GAGTAAGCAG AGACACAAGC AGAATCTTTA TGGTGACTAT 
GCTTTTGACG CCAATCGACA TGATGATAGT AGGTCAGACA ATTTCAATAC TGGAAACATG 
ACTGTTCTTT CACCATATTT AAATACTACG GTATTGCCCA GCTCTTCTTC CTCAAGGGGA 
AGTTTAGACA GTTCTCGTTC TGAGAAAGAC AGAAGTTTGG AGAGAGAGCG AGGTATTGGC 
CTCAGTGCTT ACCATCCAAC AACAGAAAAT GCAGGAACCT CATCAAAACG AGGTCTGCAG 
ATCACTACCA CTGCAGCCCA GATAGCCAAA GTTATGGAAG AAGTATCAGC CATTCATACC 
TCCCAGGACG ACAGAAGTTC TGCTTCTACC ACCGAGTTCC ATTGTGTGGC AGACGACAGG 
AGTGCGGCAC GAAGAAGCTC TGCCTCCCAC ACACACTCAA ACACATACAA CTTCACTAAG 
TCGGAAAATT CAAATAGGAC ATGCTCTATG CCTTATGCCA AAGTGGAATA TAAACGATCT 
TCAAATGACA GTTTAAATAG TGTCACTAGT AGTGATGGAT ATGGTAAAAG AGGCCAAATG 
AAACCCTCAG TTGAATCCTA TTCTGAAGAT GATGAAAGTA AATTTTGCAG TTATGGTCAG 
TATCCAGCTG ACCTAGCCCA TAAGATACAC AGTGCAAATC ATATGGATGA TAATGATGGA 
GAACTGGATA CACCAATAAA TTACAGTCTT AAATATTCAG ATGAGCAGTT GAACTCAGGA 
AGGCAGAGTC CCTCACAGAA TGAAAGGTGG GCAAGACCAA AGCATGTGAT AGAAGATGAA 
ATAAAGCAAA ACGAGCAAAG ACAAGCAAGA AGCCAGAACA CCAGTTATCC TGTCTATTCT 
GAGAATACCG ATGACAAACA CCTCAAATTC CAACCACATT TTGGACAACA AGAATGTGTT 
TCCCCATATA GGTCAAGGGG AACCAGTGGT TCAGAAACAA ATCGAATGGG TTCTAGTCAT 
GCAATTAATC AAAATGTAAA CCAGTCTCTG TGTCAGGAAG ATGATTATGA AGATGATAAA 
CCTACCAACT ACAGTGAACG TTATTCTGAG GAAGAACAAC ATGAAGAAGA AGAAGAGAGA 
CCGACAAATT ATAGCATAAA ATATAATGAA GAGAAACATC ATGTGGATCA GCCTATTGAT 
TATAGTTTAA AATATGCCAC TGACATTTCT TCCTCACAAA AACCATCATT TTCATTCTCA 
AAGAATTCAT CAGCACAAAG CACTAAACCT GAACATCTCT CTCCAAGCAG CGAGAATACA 
GCTGTACCTC CATCTAATGC CAAAAGGCAG AATCAGCTGC GTCCAAGTTC AGCACAAAGA 
AATGGCCAGA CTCAAAAAGG CACTACTTGC AAAGTCCCCT CCATCAACCA AGAAACAATA 
CAGACTTACT GCGTAGAAGA CACCCCAATA TGTTTTTCAA GGTGCAGTTC ATTATCATCA 
CTGTCATCAG CTGACGATGA AATAGGATGT GATCAGACAA CACAGGAAGC AGATTCTGCT 
AATACTCTGC AGACAGCAGA AGTAAAAGAG AATGATGTAA CTCGGTCAGC TGAAGATCCT 
GCAACTGAAG TTCCAGCAGT GTCCCAGAAT GCTAGAGCCA AACCCAGCCG ACTCCAGGCT 
TCTGGCTTAT CTTCAGAATC AACCAGGCAT AATAAAGCTG TTGAGTTTTC TTCAGGAGCC 
AAGTCTCCCT CCAAAAGTGG TGCTCAGACA CCCAAAAGTC CCCCAGAACA CTATGTCCAG 
GAGACTCCGC TCGTATTCAG CAGGTGTACT TCTGTCAGCT CCCTTGACAG TTTTGAGAGT 
CGCTCCATTG CCAGCTCTGT TCAGAGTGAG CCATGTAGTG GAATGGTGAG TGGCATCATA 
AGCCCCAGTG ACCTTCCAGA TAGTCCTGGG CAGACCATGC CACCAAGCAG AAGCAAAACC 
CCTCCACCTC CTCCACAGAC AGTGCAGGCC AAGAGAGAGG TGCCAAAAAG TAAAGTCCCT 
GCTGCTGAGA AGAGAGAGAG TGGGCCTAAG CAGACTGCTG TAAATGCTGC CGTGCAGAGG 
GTGCAGGTCC TTCCAGACGT GGATACTTTG TTACACTTCG CCACAGAAAG TACTCCAGAC 
GGGTTTTCTT GTTCCTCCAG CCTAAGTGCT CTGAGCCTGG ATGAGCCATT TATACAGAAA 
GATGTAGAAT TAAGAATCAT GCCTCCAGTT CAGGAAAACG ACAATGGGAA TGAAACTGAA 
TCAGAACAGC CTGAGGAATC AAATGAAAAC CAGGATAAAG AGGTAGAAAA GCCTGACTCT 
GAAAAAGACT TATTAGATGA TTCTGATGAC GATGATATTG AAATATTAGA AGAATGTATT 
ATTTCAGCCA TGCCAACAAA GTCATCACGC AAAGCCAAAA AACTAGCCCA GACTGCTTCA 
AAATTACCTC CACCTGTGGC AAGGAAACCA AGTCAGCTAC CTGTGTATAA ACTTCTGCCA 
GCACAGAATA GGCTGCAGGC ACAAAAACAT GTTAGCTTTA CACCAGGGGA TGATGTGCCC 
CGGGTGTACT GTGTAGAAGG GACACCTATA AACTTTTCCA CAGCAACGTC TCTAAGTGAT 
CTGACAATAG AGTCCCCTCC AAATGAATTG GCTACTGGAG ATGGGGTCAG AGCGGGTATA 
CAGTCAGGTG AATTTGAAAA ACGAGATACC ATTCCTACAG AAGGCAGAAG TACAGATGAT 
GCTCAGCGAG GAAAAATCTC ATCTATAGTT ACACCAGACC TGGATGACAA CAAAGCAGAG 
GAAGGAGATA TTCTTGCAGA ATGTATCAAT TCTGCTATGC CCAAAGGAAA AAGCCACAAG 
CCTTTCCGAG TGAAAAAGAT AATGGACCAA GTCCAACAAG CATCCTCGAC TTCATCTGGA 
GCTAACAAAA ATCAAGTAGA CACTAAGAAA AAGAAGCCTA CTTCACCAGT AAAGCCCATG 
CCACAAAATA CTGAATATAG AACGCGTGTG AGAAAGAATA CAGACTCAAA AGTTAATGTA 
AATACTGAAG AAACTTTCTC AGACAACAAA GACTCAAAGA AACCAAGCTT ACAAACCAAT 
GCCAAGGCCT TCAATGAAAA GCTACCTAAC AATGAAGACA GAGTGCGGGG GAGCTTCGCC 
TTGGACTCAC CGCATCACTA CACCCCTATT GAGGGGACGC CGTACTGCTT TTCCCGAAAT 
GACTCCTTGA GTTCTCTGGA TTTTGATGAT GACGATGTTG ACCTTTCCAG GGAAAAGGCC 
GAGTTAAGAA AGGGCAAAGA AAGCAAGGAT TCCGAAGCCA AAGTTACCTG CCGCCCAGAA 
CCAAACTCAA GCCAGCAGGC AGCTAGTAAG TCACAAGCCA GTATAAAACA TCCAGCAAAC 
AGAGCACAGT CCAAACCAGT GCTGCAGAAA CAGCCCACTT TCCCCCAGTC CTCCAAAGAC 
GGACCAGATA GAGGGGCAGC AACTGACGAA AAACTGCAGA ATTTTGCTAT TGAAAATACT 
CCAGTTTGCT TTTCTCGAAA TTCCTCTCTG AGTTCCCTTA GTGACATTGA CCAGGAAAAC 
AACAATAACA AAGAAAGTGA ACCAATCAAA GAAGCTGAAC CTGCCAACTC ACAAGGAGAG 
CCCAGTAAGC CTCAGGCATC CGGGTATGCT CCCAAGTCCT TCCACGTCGA AGACACCCCT 
GTCTGTTTCT CAAGAAACAG CTCTCTCAGT TCTCTTAGCA TTGACTCTGA GGACGACCTG 
TTACAGGAGT GTATAAGTTC TGCCATGCCA AAAAAGAAAA GGCCTTCAAG ACTCAAGAGT 
GAGAGCGAAA AGCAGAGCCC TAGAAAAGTG GGTGGCATAT TAGCTGAAGA CCTGACGCTT 
GATTTGAAAG ATCTACAGAG GCCAGATTCA GAACACGCTT TCTCCCCCGA CTCAGAAAAT 
TTTGACTGGA AAGCTATTCA GGAAGGCGCA AACTCCATAG TAAGTAGTTT GCACCAAGCT 
GCTGCAGCCG CCGCGTGCTT ATCTAGACAA GCGTCATCCG ACTCAGATTC CATTCTGTCA 
CTAAAGTCCG GCATTTCTCT GGGATCGCCT TTTCATCTTA CACCTGATCA AGAGGAAAAG 
CCATTCACAA GCAATAAAGG CCCAAGAATT CTCAAACCTG GAGAGAAAAG CACATTAGAA 
GCAAAAAAAA TAGAATCTGA AAACAAAGGA ATCAAAGGCG GGAAAAAGGT TTATAAAAGC 
TTGATTACGG GAAAGATTCG CTCCAATTCA GAAATTTCCA GCCAAATGAA ACAACCCCTC 
CCGACAAACA TGCCTTCAAT CTCAAGAGGC AGGACGATGA TTCACATCCC AGGGCTTCGG 
AATAGCTCCT CTAGTACAAG CCCTGTCTCT AAGAAAGGCC CACCCCTCAA GACTCCAGCC 
TCTAAAAGCC CCAGTGAAGG GCCGGGAGCT ACCACTTCTC CTCGAGGAAC TAAGCCAGCA 
GGAAAGTCAG AGCTTAGCCC TATCACCAGG CAAACTTCCC AAATCAGTGG GTCAAATAAG 
GGGTCTTCTA GATCAGGATC TAGAGACTCC ACTCCCTCAA GACCTACACA GCAACCATTA 
AGTAGGCCAA TGCAGTCTCC AGGGCGAAAC TCAATTTCCC CTGGTAGAAA TGGAATAAGC 
CCTCCTAACA AACTGTCTCA GCTGCCCAGA ACATCATCTC CCAGTACTGC TTCAACTAAG 
TCCTCCGGTT CTGGGAAAAT GTCATATACA TCCCCAGGTA GACAGCTGAG CCAACAAAAT 
CTTACCAAAC AAGCAAGTTT ATCCAAGAAT GCCAGCAGTA TCCCCAGAAG TGAGTCGGCA 
TCTAAAGGAC TGAATCAGAT GAGTAACGGC AATGGGTCAA ATAAAAAGGT AGAACTTTCT 
AGAATGTCTT CAACTAAATC AAGTGGAAGT GAATCAGACA GATCAGAAAG GCCTGCATTA 
GTACGCCAGT CTACTTTCAT CAAAGAAGCC CCAAGCCCAA CCCTGAGGAG GAAACTGGAG 
GAATCTGCCT CATTTGAATC CCTTTCTCCA TCTTCTAGAC CAGATTCTCC CACCAGGTCG 
CAGGCACAGA CCCCAGTTTT AAGCCCTTCC CTTCCTGATA TGTCTCTGTC CACACATCCA 
TCTGTTCAGG CAGGTGGGTG GCGAAAGCTC CCGCCTAATC TCAGCCCCAC TATCGAGTAT 
AATGACGGAA GGCCCACAAA ACGGCATGAT ATTGCACGCT CCCATTCTGA AAGTCCTTCC 
AGACTACCAA TCAACCGGGC GGGAACCTGG AAGCGTGAAC ACAGCAAACA TTCCTCGTCC 
CTTCCTCGAG TGAGTACTTG GAGAAGAACT GGAAGCTCAT CTTCTATTCT TTCTGCTTCA 
TCAGAGTCCA GTGAAAAAGC AAAAAGTGAG GATGAAAGGC ATGTGAGCTC CATGCCAGCA 
CCCAGACAGA TGAAGGAAAA CCAGGTGCCC ACCAAAGGAA CATGGAGGAA AATCAAGGAA 
AGTGACATTT CTCCCACAGG CATGGCTTCT CAGAGCGCTT CCTCAGGTGC TGCCAGTGGT 
GCTGAATCCA AGCCTCTGAT CTATCAGATG GCACCTCCTG TCTCTAAAAC AGAGGATGTT 
TGGGTGAGAA TTGAGGACTG CCCCATTAAC AACCCTAGAT CTGGACGGTC CCCCACAGGC 
AACACCCCCC CAGTGATTGA CAGTGTTTCA GAGAAGGGAA GTTCAAGCAT TAAAGATTCA 
AAAGACACCC ATGGGAAACA GAGTGTGGGC AGTGGCAGTC CTGTGCAAAC CGTGGGTCTG 
GAAACCCGCC TCAACTCCTT TGTTCAGGTA GAGGCCCCAG AACAGAAAGG AACTGAGGCA 
AAACCAGGAC AGAGTAACCC AGTCTCTATA GCAGAGACTG CTGAGACGTG TATAGCAGAG 
CGTACCCCTT TCAGTTCCAG TAGCTCCAGC AAGCACAGCT CACCTAGCGG GACTGTTGCT 
GCCAGAGTGA CACCTTTTAA TTACAACCCT AGCCCTAGGA AGAGCAGCGC AGACAGCACT 
TCAGCCCGGC CGTCTCAGAT CCCTACGCCA GTGAGCACCA ACACGAAGAA GAGAGATTCG 
AAGACTGACA GCACAGAATC CAGTGGAGCC CAAAGTCCTA AACGCCATTC CGGGTCTTAC 
CTCGTGACGT CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250285.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106145.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X15, mRNA.

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CloneID	OMu87326
Clone ID Related Accession (Same CDS sequence)	XM_030250285.1
Accession Version	XM_030250285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGT ATTGGCGCAG CTTGCCGGGG TGTCACTGGA GACAGAATGG AGGTGCTGCC 
GGGCTCGAAA ATGGGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGTCCAG CTACACGTGT GGATCACGAA ACAGCCAGTG TTTTGAGTTC TAGCGGCACG 
CACTCTGCTC CTCGAAGGTT GACAAGTCAT CTGGGGACAA AGGTGGAAAT GGTGTATTCC 
TTGTTGTCAA TGCTTGGTAC TCATGATAAG GACGATATGT CACGAACTTT GCTAGCTATG 
TCCAGCTCCC AAGACAGCTG TATATCCATG CGGCAGTCTG GATGTCTTCC TCTCCTCATC 
CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTATTGTTGG GAAATTCCCG GGGCAGTAAA 
GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC 
AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT 
GAAACCTGTT GGGAGTGGCA GGAAGCCCAC GAACAAGGCA TGGACCAGGA CAAAAACCCA 
ATGCCAGCTC CTGTTGAGCA TCAGATCTGT CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA 
TTTGATGAAG AGCATAGGCA TGCAATGAAT GAACTTGGGG GACTGCAGGC CATTGCAGAG 
TTATTGCAGG TGGACTGTGA GATGTATGGG CTTACTAATG ACCACTACAG TGTTACTTTA 
AGACGGTATG CTGGAATGGC TTTGACAAAC TTGACCTTTG GAGATGTTGC CAACAAGGCT 
ACGCTGTGTT CTATGAAAGG CTGCATGAGA GCACTTGTGG CCCAGTTAAA ATCTGAGAGT 
GAAGACTTAC AGCAGGTTAT TGCAAGTGTT TTGAGGAATT TGTCTTGGCG AGCAGATGTA 
AATAGCAAAA AGACGTTGAG AGAAGTTGGA AGTGTGAAAG CATTGATGGA ATGTGCTTTG 
GAAGTTAAAA AGGAATCAAC CCTCAAAAGC GTTTTGAGTG CCTTATGGAA CCTGTCTGCA 
CACTGCACTG AGAATAAGGC TGACATCTGT GCTGTGGATG GAGCACTGGC ATTTCTGGTT 
GGCACCCTCA CTTACCGGAG CCAGACAAAT ACTTTAGCCA TTATTGAAAG TGGAGGTGGG 
ATATTACGGA ATGTGTCCAG CTTGATAGCT ACAAACGAAG ACCACAGGCA AATCCTAAGA 
GAGAACAATT GCCTACAAAC TTTATTACAG CACTTGAAAT CTCACAGCTT GACAATAGTC 
AGTAATGCAT GTGGAACTTT GTGGAATCTC TCAGCAAGAA ATCCTAAAGA CCAGGAAGCC 
TTGTGGGACA TGGGGGCAGT GAGCATGCTC AAGAACCTCA TTCATTCCAA GCACAAAATG 
ATTGCCATGG GAAGTGCAGC AGCTTTAAGG AATCTCATGG CAAACAGACC TGCAAAGTAT 
AAGGATGCCA ATATCATGTC TCCCGGCTCA AGTCTGCCAT CCCTTCACGT TAGGAAACAG 
AAAGCTCTAG AAGCTGAGCT AGATGCTCAG CATTTATCAG AAACCTTCGA CAACATTGAC 
AACCTAAGTC CCAAGGCCTC TCACCGGAGT AAGCAGAGAC ACAAGCAGAA TCTTTATGGT 
GACTATGCTT TTGACGCCAA TCGACATGAT GATAGTAGGT CAGACAATTT CAATACTGGA 
AACATGACTG TTCTTTCACC ATATTTAAAT ACTACGGTAT TGCCCAGCTC TTCTTCCTCA 
AGGGGAAGTT TAGACAGTTC TCGTTCTGAG AAAGACAGAA GTTTGGAGAG AGAGCGAGGT 
ATTGGCCTCA GTGCTTACCA TCCAACAACA GAAAATGCAG GAACCTCATC AAAACGAGGT 
CTGCAGATCA CTACCACTGC AGCCCAGATA GCCAAAGTTA TGGAAGAAGT ATCAGCCATT 
CATACCTCCC AGGACGACAG AAGTTCTGCT TCTACCACCG AGTTCCATTG TGTGGCAGAC 
GACAGGAGTG CGGCACGAAG AAGCTCTGCC TCCCACACAC ACTCAAACAC ATACAACTTC 
ACTAAGTCGG AAAATTCAAA TAGGACATGC TCTATGCCTT ATGCCAAAGT GGAATATAAA 
CGATCTTCAA ATGACAGTTT AAATAGTGTC ACTAGTAGTG ATGGATATGG TAAAAGAGGC 
CAAATGAAAC CCTCAGTTGA ATCCTATTCT GAAGATGATG AAAGTAAATT TTGCAGTTAT 
GGTCAGTATC CAGCTGACCT AGCCCATAAG ATACACAGTG CAAATCATAT GGATGATAAT 
GATGGAGAAC TGGATACACC AATAAATTAC AGTCTTAAAT ATTCAGATGA GCAGTTGAAC 
TCAGGAAGGC AGAGTCCCTC ACAGAATGAA AGGTGGGCAA GACCAAAGCA TGTGATAGAA 
GATGAAATAA AGCAAAACGA GCAAAGACAA GCAAGAAGCC AGAACACCAG TTATCCTGTC 
TATTCTGAGA ATACCGATGA CAAACACCTC AAATTCCAAC CACATTTTGG ACAACAAGAA 
TGTGTTTCCC CATATAGGTC AAGGGGAACC AGTGGTTCAG AAACAAATCG AATGGGTTCT 
AGTCATGCAA TTAATCAAAA TGTAAACCAG TCTCTGTGTC AGGAAGATGA TTATGAAGAT 
GATAAACCTA CCAACTACAG TGAACGTTAT TCTGAGGAAG AACAACATGA AGAAGAAGAA 
GAGAGACCGA CAAATTATAG CATAAAATAT AATGAAGAGA AACATCATGT GGATCAGCCT 
ATTGATTATA GTTTAAAATA TGCCACTGAC ATTTCTTCCT CACAAAAACC ATCATTTTCA 
TTCTCAAAGA ATTCATCAGC ACAAAGCACT AAACCTGAAC ATCTCTCTCC AAGCAGCGAG 
AATACAGCTG TACCTCCATC TAATGCCAAA AGGCAGAATC AGCTGCGTCC AAGTTCAGCA 
CAAAGAAATG GCCAGACTCA AAAAGGCACT ACTTGCAAAG TCCCCTCCAT CAACCAAGAA 
ACAATACAGA CTTACTGCGT AGAAGACACC CCAATATGTT TTTCAAGGTG CAGTTCATTA 
TCATCACTGT CATCAGCTGA CGATGAAATA GGATGTGATC AGACAACACA GGAAGCAGAT 
TCTGCTAATA CTCTGCAGAC AGCAGAAGTA AAAGAGAATG ATGTAACTCG GTCAGCTGAA 
GATCCTGCAA CTGAAGTTCC AGCAGTGTCC CAGAATGCTA GAGCCAAACC CAGCCGACTC 
CAGGCTTCTG GCTTATCTTC AGAATCAACC AGGCATAATA AAGCTGTTGA GTTTTCTTCA 
GGAGCCAAGT CTCCCTCCAA AAGTGGTGCT CAGACACCCA AAAGTCCCCC AGAACACTAT 
GTCCAGGAGA CTCCGCTCGT ATTCAGCAGG TGTACTTCTG TCAGCTCCCT TGACAGTTTT 
GAGAGTCGCT CCATTGCCAG CTCTGTTCAG AGTGAGCCAT GTAGTGGAAT GGTGAGTGGC 
ATCATAAGCC CCAGTGACCT TCCAGATAGT CCTGGGCAGA CCATGCCACC AAGCAGAAGC 
AAAACCCCTC CACCTCCTCC ACAGACAGTG CAGGCCAAGA GAGAGGTGCC AAAAAGTAAA 
GTCCCTGCTG CTGAGAAGAG AGAGAGTGGG CCTAAGCAGA CTGCTGTAAA TGCTGCCGTG 
CAGAGGGTGC AGGTCCTTCC AGACGTGGAT ACTTTGTTAC ACTTCGCCAC AGAAAGTACT 
CCAGACGGGT TTTCTTGTTC CTCCAGCCTA AGTGCTCTGA GCCTGGATGA GCCATTTATA 
CAGAAAGATG TAGAATTAAG AATCATGCCT CCAGTTCAGG AAAACGACAA TGGGAATGAA 
ACTGAATCAG AACAGCCTGA GGAATCAAAT GAAAACCAGG ATAAAGAGGT AGAAAAGCCT 
GACTCTGAAA AAGACTTATT AGATGATTCT GATGACGATG ATATTGAAAT ATTAGAAGAA 
TGTATTATTT CAGCCATGCC AACAAAGTCA TCACGCAAAG CCAAAAAACT AGCCCAGACT 
GCTTCAAAAT TACCTCCACC TGTGGCAAGG AAACCAAGTC AGCTACCTGT GTATAAACTT 
CTGCCAGCAC AGAATAGGCT GCAGGCACAA AAACATGTTA GCTTTACACC AGGGGATGAT 
GTGCCCCGGG TGTACTGTGT AGAAGGGACA CCTATAAACT TTTCCACAGC AACGTCTCTA 
AGTGATCTGA CAATAGAGTC CCCTCCAAAT GAATTGGCTA CTGGAGATGG GGTCAGAGCG 
GGTATACAGT CAGGTGAATT TGAAAAACGA GATACCATTC CTACAGAAGG CAGAAGTACA 
GATGATGCTC AGCGAGGAAA AATCTCATCT ATAGTTACAC CAGACCTGGA TGACAACAAA 
GCAGAGGAAG GAGATATTCT TGCAGAATGT ATCAATTCTG CTATGCCCAA AGGAAAAAGC 
CACAAGCCTT TCCGAGTGAA AAAGATAATG GACCAAGTCC AACAAGCATC CTCGACTTCA 
TCTGGAGCTA ACAAAAATCA AGTAGACACT AAGAAAAAGA AGCCTACTTC ACCAGTAAAG 
CCCATGCCAC AAAATACTGA ATATAGAACG CGTGTGAGAA AGAATACAGA CTCAAAAGTT 
AATGTAAATA CTGAAGAAAC TTTCTCAGAC AACAAAGACT CAAAGAAACC AAGCTTACAA 
ACCAATGCCA AGGCCTTCAA TGAAAAGCTA CCTAACAATG AAGACAGAGT GCGGGGGAGC 
TTCGCCTTGG ACTCACCGCA TCACTACACC CCTATTGAGG GGACGCCGTA CTGCTTTTCC 
CGAAATGACT CCTTGAGTTC TCTGGATTTT GATGATGACG ATGTTGACCT TTCCAGGGAA 
AAGGCCGAGT TAAGAAAGGG CAAAGAAAGC AAGGATTCCG AAGCCAAAGT TACCTGCCGC 
CCAGAACCAA ACTCAAGCCA GCAGGCAGCT AGTAAGTCAC AAGCCAGTAT AAAACATCCA 
GCAAACAGAG CACAGTCCAA ACCAGTGCTG CAGAAACAGC CCACTTTCCC CCAGTCCTCC 
AAAGACGGAC CAGATAGAGG GGCAGCAACT GACGAAAAAC TGCAGAATTT TGCTATTGAA 
AATACTCCAG TTTGCTTTTC TCGAAATTCC TCTCTGAGTT CCCTTAGTGA CATTGACCAG 
GAAAACAACA ATAACAAAGA AAGTGAACCA ATCAAAGAAG CTGAACCTGC CAACTCACAA 
GGAGAGCCCA GTAAGCCTCA GGCATCCGGG TATGCTCCCA AGTCCTTCCA CGTCGAAGAC 
ACCCCTGTCT GTTTCTCAAG AAACAGCTCT CTCAGTTCTC TTAGCATTGA CTCTGAGGAC 
GACCTGTTAC AGGAGTGTAT AAGTTCTGCC ATGCCAAAAA AGAAAAGGCC TTCAAGACTC 
AAGAGTGAGA GCGAAAAGCA GAGCCCTAGA AAAGTGGGTG GCATATTAGC TGAAGACCTG 
ACGCTTGATT TGAAAGATCT ACAGAGGCCA GATTCAGAAC ACGCTTTCTC CCCCGACTCA 
GAAAATTTTG ACTGGAAAGC TATTCAGGAA GGCGCAAACT CCATAGTAAG TAGTTTGCAC 
CAAGCTGCTG CAGCCGCCGC GTGCTTATCT AGACAAGCGT CATCCGACTC AGATTCCATT 
CTGTCACTAA AGTCCGGCAT TTCTCTGGGA TCGCCTTTTC ATCTTACACC TGATCAAGAG 
GAAAAGCCAT TCACAAGCAA TAAAGGCCCA AGAATTCTCA AACCTGGAGA GAAAAGCACA 
TTAGAAGCAA AAAAAATAGA ATCTGAAAAC AAAGGAATCA AAGGCGGGAA AAAGGTTTAT 
AAAAGCTTGA TTACGGGAAA GATTCGCTCC AATTCAGAAA TTTCCAGCCA AATGAAACAA 
CCCCTCCCGA CAAACATGCC TTCAATCTCA AGAGGCAGGA CGATGATTCA CATCCCAGGG 
CTTCGGAATA GCTCCTCTAG TACAAGCCCT GTCTCTAAGA AAGGCCCACC CCTCAAGACT 
CCAGCCTCTA AAAGCCCCAG TGAAGGGCCG GGAGCTACCA CTTCTCCTCG AGGAACTAAG 
CCAGCAGGAA AGTCAGAGCT TAGCCCTATC ACCAGGCAAA CTTCCCAAAT CAGTGGGTCA 
AATAAGGGGT CTTCTAGATC AGGATCTAGA GACTCCACTC CCTCAAGACC TACACAGCAA 
CCATTAAGTA GGCCAATGCA GTCTCCAGGG CGAAACTCAA TTTCCCCTGG TAGAAATGGA 
ATAAGCCCTC CTAACAAACT GTCTCAGCTG CCCAGAACAT CATCTCCCAG TACTGCTTCA 
ACTAAGTCCT CCGGTTCTGG GAAAATGTCA TATACATCCC CAGGTAGACA GCTGAGCCAA 
CAAAATCTTA CCAAACAAGC AAGTTTATCC AAGAATGCCA GCAGTATCCC CAGAAGTGAG 
TCGGCATCTA AAGGACTGAA TCAGATGAGT AACGGCAATG GGTCAAATAA AAAGGTAGAA 
CTTTCTAGAA TGTCTTCAAC TAAATCAAGT GGAAGTGAAT CAGACAGATC AGAAAGGCCT 
GCATTAGTAC GCCAGTCTAC TTTCATCAAA GAAGCCCCAA GCCCAACCCT GAGGAGGAAA 
CTGGAGGAAT CTGCCTCATT TGAATCCCTT TCTCCATCTT CTAGACCAGA TTCTCCCACC 
AGGTCGCAGG CACAGACCCC AGTTTTAAGC CCTTCCCTTC CTGATATGTC TCTGTCCACA 
CATCCATCTG TTCAGGCAGG TGGGTGGCGA AAGCTCCCGC CTAATCTCAG CCCCACTATC 
GAGTATAATG ACGGAAGGCC CACAAAACGG CATGATATTG CACGCTCCCA TTCTGAAAGT 
CCTTCCAGAC TACCAATCAA CCGGGCGGGA ACCTGGAAGC GTGAACACAG CAAACATTCC 
TCGTCCCTTC CTCGAGTGAG TACTTGGAGA AGAACTGGAA GCTCATCTTC TATTCTTTCT 
GCTTCATCAG AGTCCAGTGA AAAAGCAAAA AGTGAGGATG AAAGGCATGT GAGCTCCATG 
CCAGCACCCA GACAGATGAA GGAAAACCAG GTGCCCACCA AAGGAACATG GAGGAAAATC 
AAGGAAAGTG ACATTTCTCC CACAGGCATG GCTTCTCAGA GCGCTTCCTC AGGTGCTGCC 
AGTGGTGCTG AATCCAAGCC TCTGATCTAT CAGATGGCAC CTCCTGTCTC TAAAACAGAG 
GATGTTTGGG TGAGAATTGA GGACTGCCCC ATTAACAACC CTAGATCTGG ACGGTCCCCC 
ACAGGCAACA CCCCCCCAGT GATTGACAGT GTTTCAGAGA AGGGAAGTTC AAGCATTAAA 
GATTCAAAAG ACACCCATGG GAAACAGAGT GTGGGCAGTG GCAGTCCTGT GCAAACCGTG 
GGTCTGGAAA CCCGCCTCAA CTCCTTTGTT CAGGTAGAGG CCCCAGAACA GAAAGGAACT 
GAGGCAAAAC CAGGACAGAG TAACCCAGTC TCTATAGCAG AGACTGCTGA GACGTGTATA 
GCAGAGCGTA CCCCTTTCAG TTCCAGTAGC TCCAGCAAGC ACAGCTCACC TAGCGGGACT 
GTTGCTGCCA GAGTGACACC TTTTAATTAC AACCCTAGCC CTAGGAAGAG CAGCGCAGAC 
AGCACTTCAG CCCGGCCGTC TCAGATCCCT ACGCCAGTGA GCACCAACAC GAAGAAGAGA 
GATTCGAAGA CTGACAGCAC AGAATCCAGT GGAGCCCAAA GTCCTAAACG CCATTCCGGG 
TCTTACCTCG TGACGTCTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106145.1
CDS	133..8496
Translation	MWLYWRSLPGCHWRQNGGAAGLENGEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAESPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250285.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250285.1

ORF Insert Sequence:

ATGTGGCTGT ATTGGCGCAG CTTGCCGGGG TGTCACTGGA GACAGAATGG AGGTGCTGCC 
GGGCTCGAAA ATGGGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGTCCAG CTACACGTGT GGATCACGAA ACAGCCAGTG TTTTGAGTTC TAGCGGCACG 
CACTCTGCTC CTCGAAGGTT GACAAGTCAT CTGGGGACAA AGGTGGAAAT GGTGTATTCC 
TTGTTGTCAA TGCTTGGTAC TCATGATAAG GACGATATGT CACGAACTTT GCTAGCTATG 
TCCAGCTCCC AAGACAGCTG TATATCCATG CGGCAGTCTG GATGTCTTCC TCTCCTCATC 
CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTATTGTTGG GAAATTCCCG GGGCAGTAAA 
GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC 
AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG AGCTTACTGT 
GAAACCTGTT GGGAGTGGCA GGAAGCCCAC GAACAAGGCA TGGACCAGGA CAAAAACCCA 
ATGCCAGCTC CTGTTGAGCA TCAGATCTGT CCTGCTGTGT GTGTTCTAAT GAAGCTTTCA 
TTTGATGAAG AGCATAGGCA TGCAATGAAT GAACTTGGGG GACTGCAGGC CATTGCAGAG 
TTATTGCAGG TGGACTGTGA GATGTATGGG CTTACTAATG ACCACTACAG TGTTACTTTA 
AGACGGTATG CTGGAATGGC TTTGACAAAC TTGACCTTTG GAGATGTTGC CAACAAGGCT 
ACGCTGTGTT CTATGAAAGG CTGCATGAGA GCACTTGTGG CCCAGTTAAA ATCTGAGAGT 
GAAGACTTAC AGCAGGTTAT TGCAAGTGTT TTGAGGAATT TGTCTTGGCG AGCAGATGTA 
AATAGCAAAA AGACGTTGAG AGAAGTTGGA AGTGTGAAAG CATTGATGGA ATGTGCTTTG 
GAAGTTAAAA AGGAATCAAC CCTCAAAAGC GTTTTGAGTG CCTTATGGAA CCTGTCTGCA 
CACTGCACTG AGAATAAGGC TGACATCTGT GCTGTGGATG GAGCACTGGC ATTTCTGGTT 
GGCACCCTCA CTTACCGGAG CCAGACAAAT ACTTTAGCCA TTATTGAAAG TGGAGGTGGG 
ATATTACGGA ATGTGTCCAG CTTGATAGCT ACAAACGAAG ACCACAGGCA AATCCTAAGA 
GAGAACAATT GCCTACAAAC TTTATTACAG CACTTGAAAT CTCACAGCTT GACAATAGTC 
AGTAATGCAT GTGGAACTTT GTGGAATCTC TCAGCAAGAA ATCCTAAAGA CCAGGAAGCC 
TTGTGGGACA TGGGGGCAGT GAGCATGCTC AAGAACCTCA TTCATTCCAA GCACAAAATG 
ATTGCCATGG GAAGTGCAGC AGCTTTAAGG AATCTCATGG CAAACAGACC TGCAAAGTAT 
AAGGATGCCA ATATCATGTC TCCCGGCTCA AGTCTGCCAT CCCTTCACGT TAGGAAACAG 
AAAGCTCTAG AAGCTGAGCT AGATGCTCAG CATTTATCAG AAACCTTCGA CAACATTGAC 
AACCTAAGTC CCAAGGCCTC TCACCGGAGT AAGCAGAGAC ACAAGCAGAA TCTTTATGGT 
GACTATGCTT TTGACGCCAA TCGACATGAT GATAGTAGGT CAGACAATTT CAATACTGGA 
AACATGACTG TTCTTTCACC ATATTTAAAT ACTACGGTAT TGCCCAGCTC TTCTTCCTCA 
AGGGGAAGTT TAGACAGTTC TCGTTCTGAG AAAGACAGAA GTTTGGAGAG AGAGCGAGGT 
ATTGGCCTCA GTGCTTACCA TCCAACAACA GAAAATGCAG GAACCTCATC AAAACGAGGT 
CTGCAGATCA CTACCACTGC AGCCCAGATA GCCAAAGTTA TGGAAGAAGT ATCAGCCATT 
CATACCTCCC AGGACGACAG AAGTTCTGCT TCTACCACCG AGTTCCATTG TGTGGCAGAC 
GACAGGAGTG CGGCACGAAG AAGCTCTGCC TCCCACACAC ACTCAAACAC ATACAACTTC 
ACTAAGTCGG AAAATTCAAA TAGGACATGC TCTATGCCTT ATGCCAAAGT GGAATATAAA 
CGATCTTCAA ATGACAGTTT AAATAGTGTC ACTAGTAGTG ATGGATATGG TAAAAGAGGC 
CAAATGAAAC CCTCAGTTGA ATCCTATTCT GAAGATGATG AAAGTAAATT TTGCAGTTAT 
GGTCAGTATC CAGCTGACCT AGCCCATAAG ATACACAGTG CAAATCATAT GGATGATAAT 
GATGGAGAAC TGGATACACC AATAAATTAC AGTCTTAAAT ATTCAGATGA GCAGTTGAAC 
TCAGGAAGGC AGAGTCCCTC ACAGAATGAA AGGTGGGCAA GACCAAAGCA TGTGATAGAA 
GATGAAATAA AGCAAAACGA GCAAAGACAA GCAAGAAGCC AGAACACCAG TTATCCTGTC 
TATTCTGAGA ATACCGATGA CAAACACCTC AAATTCCAAC CACATTTTGG ACAACAAGAA 
TGTGTTTCCC CATATAGGTC AAGGGGAACC AGTGGTTCAG AAACAAATCG AATGGGTTCT 
AGTCATGCAA TTAATCAAAA TGTAAACCAG TCTCTGTGTC AGGAAGATGA TTATGAAGAT 
GATAAACCTA CCAACTACAG TGAACGTTAT TCTGAGGAAG AACAACATGA AGAAGAAGAA 
GAGAGACCGA CAAATTATAG CATAAAATAT AATGAAGAGA AACATCATGT GGATCAGCCT 
ATTGATTATA GTTTAAAATA TGCCACTGAC ATTTCTTCCT CACAAAAACC ATCATTTTCA 
TTCTCAAAGA ATTCATCAGC ACAAAGCACT AAACCTGAAC ATCTCTCTCC AAGCAGCGAG 
AATACAGCTG TACCTCCATC TAATGCCAAA AGGCAGAATC AGCTGCGTCC AAGTTCAGCA 
CAAAGAAATG GCCAGACTCA AAAAGGCACT ACTTGCAAAG TCCCCTCCAT CAACCAAGAA 
ACAATACAGA CTTACTGCGT AGAAGACACC CCAATATGTT TTTCAAGGTG CAGTTCATTA 
TCATCACTGT CATCAGCTGA CGATGAAATA GGATGTGATC AGACAACACA GGAAGCAGAT 
TCTGCTAATA CTCTGCAGAC AGCAGAAGTA AAAGAGAATG ATGTAACTCG GTCAGCTGAA 
GATCCTGCAA CTGAAGTTCC AGCAGTGTCC CAGAATGCTA GAGCCAAACC CAGCCGACTC 
CAGGCTTCTG GCTTATCTTC AGAATCAACC AGGCATAATA AAGCTGTTGA GTTTTCTTCA 
GGAGCCAAGT CTCCCTCCAA AAGTGGTGCT CAGACACCCA AAAGTCCCCC AGAACACTAT 
GTCCAGGAGA CTCCGCTCGT ATTCAGCAGG TGTACTTCTG TCAGCTCCCT TGACAGTTTT 
GAGAGTCGCT CCATTGCCAG CTCTGTTCAG AGTGAGCCAT GTAGTGGAAT GGTGAGTGGC 
ATCATAAGCC CCAGTGACCT TCCAGATAGT CCTGGGCAGA CCATGCCACC AAGCAGAAGC 
AAAACCCCTC CACCTCCTCC ACAGACAGTG CAGGCCAAGA GAGAGGTGCC AAAAAGTAAA 
GTCCCTGCTG CTGAGAAGAG AGAGAGTGGG CCTAAGCAGA CTGCTGTAAA TGCTGCCGTG 
CAGAGGGTGC AGGTCCTTCC AGACGTGGAT ACTTTGTTAC ACTTCGCCAC AGAAAGTACT 
CCAGACGGGT TTTCTTGTTC CTCCAGCCTA AGTGCTCTGA GCCTGGATGA GCCATTTATA 
CAGAAAGATG TAGAATTAAG AATCATGCCT CCAGTTCAGG AAAACGACAA TGGGAATGAA 
ACTGAATCAG AACAGCCTGA GGAATCAAAT GAAAACCAGG ATAAAGAGGT AGAAAAGCCT 
GACTCTGAAA AAGACTTATT AGATGATTCT GATGACGATG ATATTGAAAT ATTAGAAGAA 
TGTATTATTT CAGCCATGCC AACAAAGTCA TCACGCAAAG CCAAAAAACT AGCCCAGACT 
GCTTCAAAAT TACCTCCACC TGTGGCAAGG AAACCAAGTC AGCTACCTGT GTATAAACTT 
CTGCCAGCAC AGAATAGGCT GCAGGCACAA AAACATGTTA GCTTTACACC AGGGGATGAT 
GTGCCCCGGG TGTACTGTGT AGAAGGGACA CCTATAAACT TTTCCACAGC AACGTCTCTA 
AGTGATCTGA CAATAGAGTC CCCTCCAAAT GAATTGGCTA CTGGAGATGG GGTCAGAGCG 
GGTATACAGT CAGGTGAATT TGAAAAACGA GATACCATTC CTACAGAAGG CAGAAGTACA 
GATGATGCTC AGCGAGGAAA AATCTCATCT ATAGTTACAC CAGACCTGGA TGACAACAAA 
GCAGAGGAAG GAGATATTCT TGCAGAATGT ATCAATTCTG CTATGCCCAA AGGAAAAAGC 
CACAAGCCTT TCCGAGTGAA AAAGATAATG GACCAAGTCC AACAAGCATC CTCGACTTCA 
TCTGGAGCTA ACAAAAATCA AGTAGACACT AAGAAAAAGA AGCCTACTTC ACCAGTAAAG 
CCCATGCCAC AAAATACTGA ATATAGAACG CGTGTGAGAA AGAATACAGA CTCAAAAGTT 
AATGTAAATA CTGAAGAAAC TTTCTCAGAC AACAAAGACT CAAAGAAACC AAGCTTACAA 
ACCAATGCCA AGGCCTTCAA TGAAAAGCTA CCTAACAATG AAGACAGAGT GCGGGGGAGC 
TTCGCCTTGG ACTCACCGCA TCACTACACC CCTATTGAGG GGACGCCGTA CTGCTTTTCC 
CGAAATGACT CCTTGAGTTC TCTGGATTTT GATGATGACG ATGTTGACCT TTCCAGGGAA 
AAGGCCGAGT TAAGAAAGGG CAAAGAAAGC AAGGATTCCG AAGCCAAAGT TACCTGCCGC 
CCAGAACCAA ACTCAAGCCA GCAGGCAGCT AGTAAGTCAC AAGCCAGTAT AAAACATCCA 
GCAAACAGAG CACAGTCCAA ACCAGTGCTG CAGAAACAGC CCACTTTCCC CCAGTCCTCC 
AAAGACGGAC CAGATAGAGG GGCAGCAACT GACGAAAAAC TGCAGAATTT TGCTATTGAA 
AATACTCCAG TTTGCTTTTC TCGAAATTCC TCTCTGAGTT CCCTTAGTGA CATTGACCAG 
GAAAACAACA ATAACAAAGA AAGTGAACCA ATCAAAGAAG CTGAACCTGC CAACTCACAA 
GGAGAGCCCA GTAAGCCTCA GGCATCCGGG TATGCTCCCA AGTCCTTCCA CGTCGAAGAC 
ACCCCTGTCT GTTTCTCAAG AAACAGCTCT CTCAGTTCTC TTAGCATTGA CTCTGAGGAC 
GACCTGTTAC AGGAGTGTAT AAGTTCTGCC ATGCCAAAAA AGAAAAGGCC TTCAAGACTC 
AAGAGTGAGA GCGAAAAGCA GAGCCCTAGA AAAGTGGGTG GCATATTAGC TGAAGACCTG 
ACGCTTGATT TGAAAGATCT ACAGAGGCCA GATTCAGAAC ACGCTTTCTC CCCCGACTCA 
GAAAATTTTG ACTGGAAAGC TATTCAGGAA GGCGCAAACT CCATAGTAAG TAGTTTGCAC 
CAAGCTGCTG CAGCCGCCGC GTGCTTATCT AGACAAGCGT CATCCGACTC AGATTCCATT 
CTGTCACTAA AGTCCGGCAT TTCTCTGGGA TCGCCTTTTC ATCTTACACC TGATCAAGAG 
GAAAAGCCAT TCACAAGCAA TAAAGGCCCA AGAATTCTCA AACCTGGAGA GAAAAGCACA 
TTAGAAGCAA AAAAAATAGA ATCTGAAAAC AAAGGAATCA AAGGCGGGAA AAAGGTTTAT 
AAAAGCTTGA TTACGGGAAA GATTCGCTCC AATTCAGAAA TTTCCAGCCA AATGAAACAA 
CCCCTCCCGA CAAACATGCC TTCAATCTCA AGAGGCAGGA CGATGATTCA CATCCCAGGG 
CTTCGGAATA GCTCCTCTAG TACAAGCCCT GTCTCTAAGA AAGGCCCACC CCTCAAGACT 
CCAGCCTCTA AAAGCCCCAG TGAAGGGCCG GGAGCTACCA CTTCTCCTCG AGGAACTAAG 
CCAGCAGGAA AGTCAGAGCT TAGCCCTATC ACCAGGCAAA CTTCCCAAAT CAGTGGGTCA 
AATAAGGGGT CTTCTAGATC AGGATCTAGA GACTCCACTC CCTCAAGACC TACACAGCAA 
CCATTAAGTA GGCCAATGCA GTCTCCAGGG CGAAACTCAA TTTCCCCTGG TAGAAATGGA 
ATAAGCCCTC CTAACAAACT GTCTCAGCTG CCCAGAACAT CATCTCCCAG TACTGCTTCA 
ACTAAGTCCT CCGGTTCTGG GAAAATGTCA TATACATCCC CAGGTAGACA GCTGAGCCAA 
CAAAATCTTA CCAAACAAGC AAGTTTATCC AAGAATGCCA GCAGTATCCC CAGAAGTGAG 
TCGGCATCTA AAGGACTGAA TCAGATGAGT AACGGCAATG GGTCAAATAA AAAGGTAGAA 
CTTTCTAGAA TGTCTTCAAC TAAATCAAGT GGAAGTGAAT CAGACAGATC AGAAAGGCCT 
GCATTAGTAC GCCAGTCTAC TTTCATCAAA GAAGCCCCAA GCCCAACCCT GAGGAGGAAA 
CTGGAGGAAT CTGCCTCATT TGAATCCCTT TCTCCATCTT CTAGACCAGA TTCTCCCACC 
AGGTCGCAGG CACAGACCCC AGTTTTAAGC CCTTCCCTTC CTGATATGTC TCTGTCCACA 
CATCCATCTG TTCAGGCAGG TGGGTGGCGA AAGCTCCCGC CTAATCTCAG CCCCACTATC 
GAGTATAATG ACGGAAGGCC CACAAAACGG CATGATATTG CACGCTCCCA TTCTGAAAGT 
CCTTCCAGAC TACCAATCAA CCGGGCGGGA ACCTGGAAGC GTGAACACAG CAAACATTCC 
TCGTCCCTTC CTCGAGTGAG TACTTGGAGA AGAACTGGAA GCTCATCTTC TATTCTTTCT 
GCTTCATCAG AGTCCAGTGA AAAAGCAAAA AGTGAGGATG AAAGGCATGT GAGCTCCATG 
CCAGCACCCA GACAGATGAA GGAAAACCAG GTGCCCACCA AAGGAACATG GAGGAAAATC 
AAGGAAAGTG ACATTTCTCC CACAGGCATG GCTTCTCAGA GCGCTTCCTC AGGTGCTGCC 
AGTGGTGCTG AATCCAAGCC TCTGATCTAT CAGATGGCAC CTCCTGTCTC TAAAACAGAG 
GATGTTTGGG TGAGAATTGA GGACTGCCCC ATTAACAACC CTAGATCTGG ACGGTCCCCC 
ACAGGCAACA CCCCCCCAGT GATTGACAGT GTTTCAGAGA AGGGAAGTTC AAGCATTAAA 
GATTCAAAAG ACACCCATGG GAAACAGAGT GTGGGCAGTG GCAGTCCTGT GCAAACCGTG 
GGTCTGGAAA CCCGCCTCAA CTCCTTTGTT CAGGTAGAGG CCCCAGAACA GAAAGGAACT 
GAGGCAAAAC CAGGACAGAG TAACCCAGTC TCTATAGCAG AGACTGCTGA GACGTGTATA 
GCAGAGCGTA CCCCTTTCAG TTCCAGTAGC TCCAGCAAGC ACAGCTCACC TAGCGGGACT 
GTTGCTGCCA GAGTGACACC TTTTAATTAC AACCCTAGCC CTAGGAAGAG CAGCGCAGAC 
AGCACTTCAG CCCGGCCGTC TCAGATCCCT ACGCCAGTGA GCACCAACAC GAAGAAGAGA 
GATTCGAAGA CTGACAGCAC AGAATCCAGT GGAGCCCAAA GTCCTAAACG CCATTCCGGG 
TCTTACCTCG TGACGTCTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250289.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106149.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X20, mRNA.

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CloneID	OMu87327
Clone ID Related Accession (Same CDS sequence)	XM_030250289.1 , XM_030250288.1
Accession Version	XM_030250289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGTC CAGCTACACG TGTGGATCAC GAAACAGCCA GTGTTTTGAG TTCTAGCGGC 
ACGCACTCTG CTCCTCGAAG GTTGACAAGT CATCTGGGGA CAAAGGTGGA AATGGTGTAT 
TCCTTGTTGT CAATGCTTGG TACTCATGAT AAGGACGATA TGTCACGAAC TTTGCTAGCT 
ATGTCCAGCT CCCAAGACAG CTGTATATCC ATGCGGCAGT CTGGATGTCT TCCTCTCCTC 
ATCCAGCTTT TACATGGCAA TGACAAAGAC TCTGTATTGT TGGGAAATTC CCGGGGCAGT 
AAAGAGGCTC GGGCCAGGGC CAGTGCAGCA CTCCACAACA TCATTCACTC ACAGCCTGAT 
GACAAGAGAG GCAGGCGTGA AATCCGAGTC CTTCATCTTT TGGAACAGAT ACGAGCTTAC 
TGTGAAACCT GTTGGGAGTG GCAGGAAGCC CACGAACAAG GCATGGACCA GGACAAAAAC 
CCAATGCCAG CTCCTGTTGA GCATCAGATC TGTCCTGCTG TGTGTGTTCT AATGAAGCTT 
TCATTTGATG AAGAGCATAG GCATGCAATG AATGAACTTG GTAGGAAGGC TACCCGGGGC 
ATTTCATCCC AGGAGCTAGG TCAGGGGCTT TCAGGGGGAC TGCAGGCCAT TGCAGAGTTA 
TTGCAGGTGG ACTGTGAGAT GTATGGGCTT ACTAATGACC ACTACAGTGT TACTTTAAGA 
CGGTATGCTG GAATGGCTTT GACAAACTTG ACCTTTGGAG ATGTTGCCAA CAAGGCTACG 
CTGTGTTCTA TGAAAGGCTG CATGAGAGCA CTTGTGGCCC AGTTAAAATC TGAGAGTGAA 
GACTTACAGC AGGTTATTGC AAGTGTTTTG AGGAATTTGT CTTGGCGAGC AGATGTAAAT 
AGCAAAAAGA CGTTGAGAGA AGTTGGAAGT GTGAAAGCAT TGATGGAATG TGCTTTGGAA 
GTTAAAAAGG AATCAACCCT CAAAAGCGTT TTGAGTGCCT TATGGAACCT GTCTGCACAC 
TGCACTGAGA ATAAGGCTGA CATCTGTGCT GTGGATGGAG CACTGGCATT TCTGGTTGGC 
ACCCTCACTT ACCGGAGCCA GACAAATACT TTAGCCATTA TTGAAAGTGG AGGTGGGATA 
TTACGGAATG TGTCCAGCTT GATAGCTACA AACGAAGACC ACAGGCAAAT CCTAAGAGAG 
AACAATTGCC TACAAACTTT ATTACAGCAC TTGAAATCTC ACAGCTTGAC AATAGTCAGT 
AATGCATGTG GAACTTTGTG GAATCTCTCA GCAAGAAATC CTAAAGACCA GGAAGCCTTG 
TGGGACATGG GGGCAGTGAG CATGCTCAAG AACCTCATTC ATTCCAAGCA CAAAATGATT 
GCCATGGGAA GTGCAGCAGC TTTAAGGAAT CTCATGGCAA ACAGACCTGC AAAGTATAAG 
GATGCCAATA TCATGTCTCC CGGCTCAAGT CTGCCATCCC TTCACGTTAG GAAACAGAAA 
GCTCTAGAAG CTGAGCTAGA TGCTCAGCAT TTATCAGAAA CCTTCGACAA CATTGACAAC 
CTAAGTCCCA AGGCCTCTCA CCGGAGTAAG CAGAGACACA AGCAGAATCT TTATGGTGAC 
TATGCTTTTG ACGCCAATCG ACATGATGAT AGTAGGTCAG ACAATTTCAA TACTGGAAAC 
ATGACTGTTC TTTCACCATA TTTAAATACT ACGGTATTGC CCAGCTCTTC TTCCTCAAGG 
GGAAGTTTAG ACAGTTCTCG TTCTGAGAAA GACAGAAGTT TGGAGAGAGA GCGAGGTATT 
GGCCTCAGTG CTTACCATCC AACAACAGAA AATGCAGGAA CCTCATCAAA ACGAGGTCTG 
CAGATCACTA CCACTGCAGC CCAGATAGCC AAAGTTATGG AAGAAGTATC AGCCATTCAT 
ACCTCCCAGG ACGACAGAAG TTCTGCTTCT ACCACCGAGT TCCATTGTGT GGCAGACGAC 
AGGAGTGCGG CACGAAGAAG CTCTGCCTCC CACACACACT CAAACACATA CAACTTCACT 
AAGTCGGAAA ATTCAAATAG GACATGCTCT ATGCCTTATG CCAAAGTGGA ATATAAACGA 
TCTTCAAATG ACAGTTTAAA TAGTGTCACT AGTAGTGATG GATATGGTAA AAGAGGCCAA 
ATGAAACCCT CAGTTGAATC CTATTCTGAA GATGATGAAA GTAAATTTTG CAGTTATGGT 
CAGTATCCAG CTGACCTAGC CCATAAGATA CACAGTGCAA ATCATATGGA TGATAATGAT 
GGAGAACTGG ATACACCAAT AAATTACAGT CTTAAATATT CAGATGAGCA GTTGAACTCA 
GGAAGGCAGA GTCCCTCACA GAATGAAAGG TGGGCAAGAC CAAAGCATGT GATAGAAGAT 
GAAATAAAGC AAAACGAGCA AAGACAAGCA AGAAGCCAGA ACACCAGTTA TCCTGTCTAT 
TCTGAGAATA CCGATGACAA ACACCTCAAA TTCCAACCAC ATTTTGGACA ACAAGAATGT 
GTTTCCCCAT ATAGGTCAAG GGGAACCAGT GGTTCAGAAA CAAATCGAAT GGGTTCTAGT 
CATGCAATTA ATCAAAATGT AAACCAGTCT CTGTGTCAGG AAGATGATTA TGAAGATGAT 
AAACCTACCA ACTACAGTGA ACGTTATTCT GAGGAAGAAC AACATGAAGA AGAAGAAGAG 
AGACCGACAA ATTATAGCAT AAAATATAAT GAAGAGAAAC ATCATGTGGA TCAGCCTATT 
GATTATAGTT TAAAATATGC CACTGACATT TCTTCCTCAC AAAAACCATC ATTTTCATTC 
TCAAAGAATT CATCAGCACA AAGCACTAAA CCTGAACATC TCTCTCCAAG CAGCGAGAAT 
ACAGCTGTAC CTCCATCTAA TGCCAAAAGG CAGAATCAGC TGCGTCCAAG TTCAGCACAA 
AGAAATGGCC AGACTCAAAA AGGCACTACT TGCAAAGTCC CCTCCATCAA CCAAGAAACA 
ATACAGACTT ACTGCGTAGA AGACACCCCA ATATGTTTTT CAAGGTGCAG TTCATTATCA 
TCACTGTCAT CAGCTGACGA TGAAATAGGA TGTGATCAGA CAACACAGGA AGCAGATTCT 
GCTAATACTC TGCAGACAGC AGAAGTAAAA GAGAATGATG TAACTCGGTC AGCTGAAGAT 
CCTGCAACTG AAGTTCCAGC AGTGTCCCAG AATGCTAGAG CCAAACCCAG CCGACTCCAG 
GCTTCTGGCT TATCTTCAGA ATCAACCAGG CATAATAAAG CTGTTGAGTT TTCTTCAGGA 
GCCAAGTCTC CCTCCAAAAG TGGTGCTCAG ACACCCAAAA GTCCCCCAGA ACACTATGTC 
CAGGAGACTC CGCTCGTATT CAGCAGGTGT ACTTCTGTCA GCTCCCTTGA CAGTTTTGAG 
AGTCGCTCCA TTGCCAGCTC TGTTCAGAGT GAGCCATGTA GTGGAATGGT GAGTGGCATC 
ATAAGCCCCA GTGACCTTCC AGATAGTCCT GGGCAGACCA TGCCACCAAG CAGAAGCAAA 
ACCCCTCCAC CTCCTCCACA GACAGTGCAG GCCAAGAGAG AGGTGCCAAA AAGTAAAGTC 
CCTGCTGCTG AGAAGAGAGA GAGTGGGCCT AAGCAGACTG CTGTAAATGC TGCCGTGCAG 
AGGGTGCAGG TCCTTCCAGA CGTGGATACT TTGTTACACT TCGCCACAGA AAGTACTCCA 
GACGGGTTTT CTTGTTCCTC CAGCCTAAGT GCTCTGAGCC TGGATGAGCC ATTTATACAG 
AAAGATGTAG AATTAAGAAT CATGCCTCCA GTTCAGGAAA ACGACAATGG GAATGAAACT 
GAATCAGAAC AGCCTGAGGA ATCAAATGAA AACCAGGATA AAGAGGTAGA AAAGCCTGAC 
TCTGAAAAAG ACTTATTAGA TGATTCTGAT GACGATGATA TTGAAATATT AGAAGAATGT 
ATTATTTCAG CCATGCCAAC AAAGTCATCA CGCAAAGCCA AAAAACTAGC CCAGACTGCT 
TCAAAATTAC CTCCACCTGT GGCAAGGAAA CCAAGTCAGC TACCTGTGTA TAAACTTCTG 
CCAGCACAGA ATAGGCTGCA GGCACAAAAA CATGTTAGCT TTACACCAGG GGATGATGTG 
CCCCGGGTGT ACTGTGTAGA AGGGACACCT ATAAACTTTT CCACAGCAAC GTCTCTAAGT 
GATCTGACAA TAGAGTCCCC TCCAAATGAA TTGGCTACTG GAGATGGGGT CAGAGCGGGT 
ATACAGTCAG GTGAATTTGA AAAACGAGAT ACCATTCCTA CAGAAGGCAG AAGTACAGAT 
GATGCTCAGC GAGGAAAAAT CTCATCTATA GTTACACCAG ACCTGGATGA CAACAAAGCA 
GAGGAAGGAG ATATTCTTGC AGAATGTATC AATTCTGCTA TGCCCAAAGG AAAAAGCCAC 
AAGCCTTTCC GAGTGAAAAA GATAATGGAC CAAGTCCAAC AAGCATCCTC GACTTCATCT 
GGAGCTAACA AAAATCAAGT AGACACTAAG AAAAAGAAGC CTACTTCACC AGTAAAGCCC 
ATGCCACAAA ATACTGAATA TAGAACGCGT GTGAGAAAGA ATACAGACTC AAAAGTTAAT 
GTAAATACTG AAGAAACTTT CTCAGACAAC AAAGACTCAA AGAAACCAAG CTTACAAACC 
AATGCCAAGG CCTTCAATGA AAAGCTACCT AACAATGAAG ACAGAGTGCG GGGGAGCTTC 
GCCTTGGACT CACCGCATCA CTACACCCCT ATTGAGGGGA CGCCGTACTG CTTTTCCCGA 
AATGACTCCT TGAGTTCTCT GGATTTTGAT GATGACGATG TTGACCTTTC CAGGGAAAAG 
GCCGAGTTAA GAAAGGGCAA AGAAAGCAAG GATTCCGAAG CCAAAGTTAC CTGCCGCCCA 
GAACCAAACT CAAGCCAGCA GGCAGCTAGT AAGTCACAAG CCAGTATAAA ACATCCAGCA 
AACAGAGCAC AGTCCAAACC AGTGCTGCAG AAACAGCCCA CTTTCCCCCA GTCCTCCAAA 
GACGGACCAG ATAGAGGGGC AGCAACTGAC GAAAAACTGC AGAATTTTGC TATTGAAAAT 
ACTCCAGTTT GCTTTTCTCG AAATTCCTCT CTGAGTTCCC TTAGTGACAT TGACCAGGAA 
AACAACAATA ACAAAGAAAG TGAACCAATC AAAGAAGCTG AACCTGCCAA CTCACAAGGA 
GAGCCCAGTA AGCCTCAGGC ATCCGGGTAT GCTCCCAAGT CCTTCCACGT CGAAGACACC 
CCTGTCTGTT TCTCAAGAAA CAGCTCTCTC AGTTCTCTTA GCATTGACTC TGAGGACGAC 
CTGTTACAGG AGTGTATAAG TTCTGCCATG CCAAAAAAGA AAAGGCCTTC AAGACTCAAG 
AGTGAGAGCG AAAAGCAGAG CCCTAGAAAA GTGGGTGGCA TATTAGCTGA AGACCTGACG 
CTTGATTTGA AAGATCTACA GAGGCCAGAT TCAGAACACG CTTTCTCCCC CGACTCAGAA 
AATTTTGACT GGAAAGCTAT TCAGGAAGGC GCAAACTCCA TAGTAAGTAG TTTGCACCAA 
GCTGCTGCAG CCGCCGCGTG CTTATCTAGA CAAGCGTCAT CCGACTCAGA TTCCATTCTG 
TCACTAAAGT CCGGCATTTC TCTGGGATCG CCTTTTCATC TTACACCTGA TCAAGAGGAA 
AAGCCATTCA CAAGCAATAA AGGCCCAAGA ATTCTCAAAC CTGGAGAGAA AAGCACATTA 
GAAGCAAAAA AAATAGAATC TGAAAACAAA GGAATCAAAG GCGGGAAAAA GGTTTATAAA 
AGCTTGATTA CGGGAAAGAT TCGCTCCAAT TCAGAAATTT CCAGCCAAAT GAAACAACCC 
CTCCCGACAA ACATGCCTTC AATCTCAAGA GGCAGGACGA TGATTCACAT CCCAGGGCTT 
CGGAATAGCT CCTCTAGTAC AAGCCCTGTC TCTAAGAAAG GCCCACCCCT CAAGACTCCA 
GCCTCTAAAA GCCCCAGTGA AGGGCCGGGA GCTACCACTT CTCCTCGAGG AACTAAGCCA 
GCAGGAAAGT CAGAGCTTAG CCCTATCACC AGGCAAACTT CCCAAATCAG TGGGTCAAAT 
AAGGGGTCTT CTAGATCAGG ATCTAGAGAC TCCACTCCCT CAAGACCTAC ACAGCAACCA 
TTAAGTAGGC CAATGCAGTC TCCAGGGCGA AACTCAATTT CCCCTGGTAG AAATGGAATA 
AGCCCTCCTA ACAAACTGTC TCAGCTGCCC AGAACATCAT CTCCCAGTAC TGCTTCAACT 
AAGTCCTCCG GTTCTGGGAA AATGTCATAT ACATCCCCAG GTAGACAGCT GAGCCAACAA 
AATCTTACCA AACAAGCAAG TTTATCCAAG AATGCCAGCA GTATCCCCAG AAGTGAGTCG 
GCATCTAAAG GACTGAATCA GATGAGTAAC GGCAATGGGT CAAATAAAAA GGTAGAACTT 
TCTAGAATGT CTTCAACTAA ATCAAGTGGA AGTGAATCAG ACAGATCAGA AAGGCCTGCA 
TTAGTACGCC AGTCTACTTT CATCAAAGAA GCCCCAAGCC CAACCCTGAG GAGGAAACTG 
GAGGAATCTG CCTCATTTGA ATCCCTTTCT CCATCTTCTA GACCAGATTC TCCCACCAGG 
TCGCAGGCAC AGACCCCAGT TTTAAGCCCT TCCCTTCCTG ATATGTCTCT GTCCACACAT 
CCATCTGTTC AGGCAGGTGG GTGGCGAAAG CTCCCGCCTA ATCTCAGCCC CACTATCGAG 
TATAATGACG GAAGGCCCAC AAAACGGCAT GATATTGCAC GCTCCCATTC TGAAAGTCCT 
TCCAGACTAC CAATCAACCG GGCGGGAACC TGGAAGCGTG AACACAGCAA ACATTCCTCG 
TCCCTTCCTC GAGTGAGTAC TTGGAGAAGA ACTGGAAGCT CATCTTCTAT TCTTTCTGCT 
TCATCAGAGT CCAGTGAAAA AGCAAAAAGT GAGGATGAAA GGCATGTGAG CTCCATGCCA 
GCACCCAGAC AGATGAAGGA AAACCAGGTG CCCACCAAAG GAACATGGAG GAAAATCAAG 
GAAAGTGACA TTTCTCCCAC AGGCATGGCT TCTCAGAGCG CTTCCTCAGG TGCTGCCAGT 
GGTGCTGAAT CCAAGCCTCT GATCTATCAG ATGGCACCTC CTGTCTCTAA AACAGAGGAT 
GTTTGGGTGA GAATTGAGGA CTGCCCCATT AACAACCCTA GATCTGGACG GTCCCCCACA 
GGCAACACCC CCCCAGTGAT TGACAGTGTT TCAGAGAAGG GAAGTTCAAG CATTAAAGAT 
TCAAAAGACA CCCATGGGAA ACAGAGTGTG GGCAGTGGCA GTCCTGTGCA AACCGTGGGT 
CTGGAAACCC GCCTCAACTC CTTTGTTCAG GTAGAGGCCC CAGAACAGAA AGGAACTGAG 
GCAAAACCAG GACAGAGTAA CCCAGTCTCT ATAGCAGAGA CTGCTGAGAC GTGTATAGCA 
GAGCGTACCC CTTTCAGTTC CAGTAGCTCC AGCAAGCACA GCTCACCTAG CGGGACTGTT 
GCTGCCAGAG TGACACCTTT TAATTACAAC CCTAGCCCTA GGAAGAGCAG CGCAGACAGC 
ACTTCAGCCC GGCCGTCTCA GATCCCTACG CCAGTGAGCA CCAACACGAA GAAGAGAGAT 
TCGAAGACTG ACAGCACAGA ATCCAGTGGA GCCCAAAGTC CTAAACGCCA TTCCGGGTCT 
TACCTCGTGA CGTCTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106149.1
CDS	294..8594
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAESPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250289.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250289.1

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGTC CAGCTACACG TGTGGATCAC GAAACAGCCA GTGTTTTGAG TTCTAGCGGC 
ACGCACTCTG CTCCTCGAAG GTTGACAAGT CATCTGGGGA CAAAGGTGGA AATGGTGTAT 
TCCTTGTTGT CAATGCTTGG TACTCATGAT AAGGACGATA TGTCACGAAC TTTGCTAGCT 
ATGTCCAGCT CCCAAGACAG CTGTATATCC ATGCGGCAGT CTGGATGTCT TCCTCTCCTC 
ATCCAGCTTT TACATGGCAA TGACAAAGAC TCTGTATTGT TGGGAAATTC CCGGGGCAGT 
AAAGAGGCTC GGGCCAGGGC CAGTGCAGCA CTCCACAACA TCATTCACTC ACAGCCTGAT 
GACAAGAGAG GCAGGCGTGA AATCCGAGTC CTTCATCTTT TGGAACAGAT ACGAGCTTAC 
TGTGAAACCT GTTGGGAGTG GCAGGAAGCC CACGAACAAG GCATGGACCA GGACAAAAAC 
CCAATGCCAG CTCCTGTTGA GCATCAGATC TGTCCTGCTG TGTGTGTTCT AATGAAGCTT 
TCATTTGATG AAGAGCATAG GCATGCAATG AATGAACTTG GTAGGAAGGC TACCCGGGGC 
ATTTCATCCC AGGAGCTAGG TCAGGGGCTT TCAGGGGGAC TGCAGGCCAT TGCAGAGTTA 
TTGCAGGTGG ACTGTGAGAT GTATGGGCTT ACTAATGACC ACTACAGTGT TACTTTAAGA 
CGGTATGCTG GAATGGCTTT GACAAACTTG ACCTTTGGAG ATGTTGCCAA CAAGGCTACG 
CTGTGTTCTA TGAAAGGCTG CATGAGAGCA CTTGTGGCCC AGTTAAAATC TGAGAGTGAA 
GACTTACAGC AGGTTATTGC AAGTGTTTTG AGGAATTTGT CTTGGCGAGC AGATGTAAAT 
AGCAAAAAGA CGTTGAGAGA AGTTGGAAGT GTGAAAGCAT TGATGGAATG TGCTTTGGAA 
GTTAAAAAGG AATCAACCCT CAAAAGCGTT TTGAGTGCCT TATGGAACCT GTCTGCACAC 
TGCACTGAGA ATAAGGCTGA CATCTGTGCT GTGGATGGAG CACTGGCATT TCTGGTTGGC 
ACCCTCACTT ACCGGAGCCA GACAAATACT TTAGCCATTA TTGAAAGTGG AGGTGGGATA 
TTACGGAATG TGTCCAGCTT GATAGCTACA AACGAAGACC ACAGGCAAAT CCTAAGAGAG 
AACAATTGCC TACAAACTTT ATTACAGCAC TTGAAATCTC ACAGCTTGAC AATAGTCAGT 
AATGCATGTG GAACTTTGTG GAATCTCTCA GCAAGAAATC CTAAAGACCA GGAAGCCTTG 
TGGGACATGG GGGCAGTGAG CATGCTCAAG AACCTCATTC ATTCCAAGCA CAAAATGATT 
GCCATGGGAA GTGCAGCAGC TTTAAGGAAT CTCATGGCAA ACAGACCTGC AAAGTATAAG 
GATGCCAATA TCATGTCTCC CGGCTCAAGT CTGCCATCCC TTCACGTTAG GAAACAGAAA 
GCTCTAGAAG CTGAGCTAGA TGCTCAGCAT TTATCAGAAA CCTTCGACAA CATTGACAAC 
CTAAGTCCCA AGGCCTCTCA CCGGAGTAAG CAGAGACACA AGCAGAATCT TTATGGTGAC 
TATGCTTTTG ACGCCAATCG ACATGATGAT AGTAGGTCAG ACAATTTCAA TACTGGAAAC 
ATGACTGTTC TTTCACCATA TTTAAATACT ACGGTATTGC CCAGCTCTTC TTCCTCAAGG 
GGAAGTTTAG ACAGTTCTCG TTCTGAGAAA GACAGAAGTT TGGAGAGAGA GCGAGGTATT 
GGCCTCAGTG CTTACCATCC AACAACAGAA AATGCAGGAA CCTCATCAAA ACGAGGTCTG 
CAGATCACTA CCACTGCAGC CCAGATAGCC AAAGTTATGG AAGAAGTATC AGCCATTCAT 
ACCTCCCAGG ACGACAGAAG TTCTGCTTCT ACCACCGAGT TCCATTGTGT GGCAGACGAC 
AGGAGTGCGG CACGAAGAAG CTCTGCCTCC CACACACACT CAAACACATA CAACTTCACT 
AAGTCGGAAA ATTCAAATAG GACATGCTCT ATGCCTTATG CCAAAGTGGA ATATAAACGA 
TCTTCAAATG ACAGTTTAAA TAGTGTCACT AGTAGTGATG GATATGGTAA AAGAGGCCAA 
ATGAAACCCT CAGTTGAATC CTATTCTGAA GATGATGAAA GTAAATTTTG CAGTTATGGT 
CAGTATCCAG CTGACCTAGC CCATAAGATA CACAGTGCAA ATCATATGGA TGATAATGAT 
GGAGAACTGG ATACACCAAT AAATTACAGT CTTAAATATT CAGATGAGCA GTTGAACTCA 
GGAAGGCAGA GTCCCTCACA GAATGAAAGG TGGGCAAGAC CAAAGCATGT GATAGAAGAT 
GAAATAAAGC AAAACGAGCA AAGACAAGCA AGAAGCCAGA ACACCAGTTA TCCTGTCTAT 
TCTGAGAATA CCGATGACAA ACACCTCAAA TTCCAACCAC ATTTTGGACA ACAAGAATGT 
GTTTCCCCAT ATAGGTCAAG GGGAACCAGT GGTTCAGAAA CAAATCGAAT GGGTTCTAGT 
CATGCAATTA ATCAAAATGT AAACCAGTCT CTGTGTCAGG AAGATGATTA TGAAGATGAT 
AAACCTACCA ACTACAGTGA ACGTTATTCT GAGGAAGAAC AACATGAAGA AGAAGAAGAG 
AGACCGACAA ATTATAGCAT AAAATATAAT GAAGAGAAAC ATCATGTGGA TCAGCCTATT 
GATTATAGTT TAAAATATGC CACTGACATT TCTTCCTCAC AAAAACCATC ATTTTCATTC 
TCAAAGAATT CATCAGCACA AAGCACTAAA CCTGAACATC TCTCTCCAAG CAGCGAGAAT 
ACAGCTGTAC CTCCATCTAA TGCCAAAAGG CAGAATCAGC TGCGTCCAAG TTCAGCACAA 
AGAAATGGCC AGACTCAAAA AGGCACTACT TGCAAAGTCC CCTCCATCAA CCAAGAAACA 
ATACAGACTT ACTGCGTAGA AGACACCCCA ATATGTTTTT CAAGGTGCAG TTCATTATCA 
TCACTGTCAT CAGCTGACGA TGAAATAGGA TGTGATCAGA CAACACAGGA AGCAGATTCT 
GCTAATACTC TGCAGACAGC AGAAGTAAAA GAGAATGATG TAACTCGGTC AGCTGAAGAT 
CCTGCAACTG AAGTTCCAGC AGTGTCCCAG AATGCTAGAG CCAAACCCAG CCGACTCCAG 
GCTTCTGGCT TATCTTCAGA ATCAACCAGG CATAATAAAG CTGTTGAGTT TTCTTCAGGA 
GCCAAGTCTC CCTCCAAAAG TGGTGCTCAG ACACCCAAAA GTCCCCCAGA ACACTATGTC 
CAGGAGACTC CGCTCGTATT CAGCAGGTGT ACTTCTGTCA GCTCCCTTGA CAGTTTTGAG 
AGTCGCTCCA TTGCCAGCTC TGTTCAGAGT GAGCCATGTA GTGGAATGGT GAGTGGCATC 
ATAAGCCCCA GTGACCTTCC AGATAGTCCT GGGCAGACCA TGCCACCAAG CAGAAGCAAA 
ACCCCTCCAC CTCCTCCACA GACAGTGCAG GCCAAGAGAG AGGTGCCAAA AAGTAAAGTC 
CCTGCTGCTG AGAAGAGAGA GAGTGGGCCT AAGCAGACTG CTGTAAATGC TGCCGTGCAG 
AGGGTGCAGG TCCTTCCAGA CGTGGATACT TTGTTACACT TCGCCACAGA AAGTACTCCA 
GACGGGTTTT CTTGTTCCTC CAGCCTAAGT GCTCTGAGCC TGGATGAGCC ATTTATACAG 
AAAGATGTAG AATTAAGAAT CATGCCTCCA GTTCAGGAAA ACGACAATGG GAATGAAACT 
GAATCAGAAC AGCCTGAGGA ATCAAATGAA AACCAGGATA AAGAGGTAGA AAAGCCTGAC 
TCTGAAAAAG ACTTATTAGA TGATTCTGAT GACGATGATA TTGAAATATT AGAAGAATGT 
ATTATTTCAG CCATGCCAAC AAAGTCATCA CGCAAAGCCA AAAAACTAGC CCAGACTGCT 
TCAAAATTAC CTCCACCTGT GGCAAGGAAA CCAAGTCAGC TACCTGTGTA TAAACTTCTG 
CCAGCACAGA ATAGGCTGCA GGCACAAAAA CATGTTAGCT TTACACCAGG GGATGATGTG 
CCCCGGGTGT ACTGTGTAGA AGGGACACCT ATAAACTTTT CCACAGCAAC GTCTCTAAGT 
GATCTGACAA TAGAGTCCCC TCCAAATGAA TTGGCTACTG GAGATGGGGT CAGAGCGGGT 
ATACAGTCAG GTGAATTTGA AAAACGAGAT ACCATTCCTA CAGAAGGCAG AAGTACAGAT 
GATGCTCAGC GAGGAAAAAT CTCATCTATA GTTACACCAG ACCTGGATGA CAACAAAGCA 
GAGGAAGGAG ATATTCTTGC AGAATGTATC AATTCTGCTA TGCCCAAAGG AAAAAGCCAC 
AAGCCTTTCC GAGTGAAAAA GATAATGGAC CAAGTCCAAC AAGCATCCTC GACTTCATCT 
GGAGCTAACA AAAATCAAGT AGACACTAAG AAAAAGAAGC CTACTTCACC AGTAAAGCCC 
ATGCCACAAA ATACTGAATA TAGAACGCGT GTGAGAAAGA ATACAGACTC AAAAGTTAAT 
GTAAATACTG AAGAAACTTT CTCAGACAAC AAAGACTCAA AGAAACCAAG CTTACAAACC 
AATGCCAAGG CCTTCAATGA AAAGCTACCT AACAATGAAG ACAGAGTGCG GGGGAGCTTC 
GCCTTGGACT CACCGCATCA CTACACCCCT ATTGAGGGGA CGCCGTACTG CTTTTCCCGA 
AATGACTCCT TGAGTTCTCT GGATTTTGAT GATGACGATG TTGACCTTTC CAGGGAAAAG 
GCCGAGTTAA GAAAGGGCAA AGAAAGCAAG GATTCCGAAG CCAAAGTTAC CTGCCGCCCA 
GAACCAAACT CAAGCCAGCA GGCAGCTAGT AAGTCACAAG CCAGTATAAA ACATCCAGCA 
AACAGAGCAC AGTCCAAACC AGTGCTGCAG AAACAGCCCA CTTTCCCCCA GTCCTCCAAA 
GACGGACCAG ATAGAGGGGC AGCAACTGAC GAAAAACTGC AGAATTTTGC TATTGAAAAT 
ACTCCAGTTT GCTTTTCTCG AAATTCCTCT CTGAGTTCCC TTAGTGACAT TGACCAGGAA 
AACAACAATA ACAAAGAAAG TGAACCAATC AAAGAAGCTG AACCTGCCAA CTCACAAGGA 
GAGCCCAGTA AGCCTCAGGC ATCCGGGTAT GCTCCCAAGT CCTTCCACGT CGAAGACACC 
CCTGTCTGTT TCTCAAGAAA CAGCTCTCTC AGTTCTCTTA GCATTGACTC TGAGGACGAC 
CTGTTACAGG AGTGTATAAG TTCTGCCATG CCAAAAAAGA AAAGGCCTTC AAGACTCAAG 
AGTGAGAGCG AAAAGCAGAG CCCTAGAAAA GTGGGTGGCA TATTAGCTGA AGACCTGACG 
CTTGATTTGA AAGATCTACA GAGGCCAGAT TCAGAACACG CTTTCTCCCC CGACTCAGAA 
AATTTTGACT GGAAAGCTAT TCAGGAAGGC GCAAACTCCA TAGTAAGTAG TTTGCACCAA 
GCTGCTGCAG CCGCCGCGTG CTTATCTAGA CAAGCGTCAT CCGACTCAGA TTCCATTCTG 
TCACTAAAGT CCGGCATTTC TCTGGGATCG CCTTTTCATC TTACACCTGA TCAAGAGGAA 
AAGCCATTCA CAAGCAATAA AGGCCCAAGA ATTCTCAAAC CTGGAGAGAA AAGCACATTA 
GAAGCAAAAA AAATAGAATC TGAAAACAAA GGAATCAAAG GCGGGAAAAA GGTTTATAAA 
AGCTTGATTA CGGGAAAGAT TCGCTCCAAT TCAGAAATTT CCAGCCAAAT GAAACAACCC 
CTCCCGACAA ACATGCCTTC AATCTCAAGA GGCAGGACGA TGATTCACAT CCCAGGGCTT 
CGGAATAGCT CCTCTAGTAC AAGCCCTGTC TCTAAGAAAG GCCCACCCCT CAAGACTCCA 
GCCTCTAAAA GCCCCAGTGA AGGGCCGGGA GCTACCACTT CTCCTCGAGG AACTAAGCCA 
GCAGGAAAGT CAGAGCTTAG CCCTATCACC AGGCAAACTT CCCAAATCAG TGGGTCAAAT 
AAGGGGTCTT CTAGATCAGG ATCTAGAGAC TCCACTCCCT CAAGACCTAC ACAGCAACCA 
TTAAGTAGGC CAATGCAGTC TCCAGGGCGA AACTCAATTT CCCCTGGTAG AAATGGAATA 
AGCCCTCCTA ACAAACTGTC TCAGCTGCCC AGAACATCAT CTCCCAGTAC TGCTTCAACT 
AAGTCCTCCG GTTCTGGGAA AATGTCATAT ACATCCCCAG GTAGACAGCT GAGCCAACAA 
AATCTTACCA AACAAGCAAG TTTATCCAAG AATGCCAGCA GTATCCCCAG AAGTGAGTCG 
GCATCTAAAG GACTGAATCA GATGAGTAAC GGCAATGGGT CAAATAAAAA GGTAGAACTT 
TCTAGAATGT CTTCAACTAA ATCAAGTGGA AGTGAATCAG ACAGATCAGA AAGGCCTGCA 
TTAGTACGCC AGTCTACTTT CATCAAAGAA GCCCCAAGCC CAACCCTGAG GAGGAAACTG 
GAGGAATCTG CCTCATTTGA ATCCCTTTCT CCATCTTCTA GACCAGATTC TCCCACCAGG 
TCGCAGGCAC AGACCCCAGT TTTAAGCCCT TCCCTTCCTG ATATGTCTCT GTCCACACAT 
CCATCTGTTC AGGCAGGTGG GTGGCGAAAG CTCCCGCCTA ATCTCAGCCC CACTATCGAG 
TATAATGACG GAAGGCCCAC AAAACGGCAT GATATTGCAC GCTCCCATTC TGAAAGTCCT 
TCCAGACTAC CAATCAACCG GGCGGGAACC TGGAAGCGTG AACACAGCAA ACATTCCTCG 
TCCCTTCCTC GAGTGAGTAC TTGGAGAAGA ACTGGAAGCT CATCTTCTAT TCTTTCTGCT 
TCATCAGAGT CCAGTGAAAA AGCAAAAAGT GAGGATGAAA GGCATGTGAG CTCCATGCCA 
GCACCCAGAC AGATGAAGGA AAACCAGGTG CCCACCAAAG GAACATGGAG GAAAATCAAG 
GAAAGTGACA TTTCTCCCAC AGGCATGGCT TCTCAGAGCG CTTCCTCAGG TGCTGCCAGT 
GGTGCTGAAT CCAAGCCTCT GATCTATCAG ATGGCACCTC CTGTCTCTAA AACAGAGGAT 
GTTTGGGTGA GAATTGAGGA CTGCCCCATT AACAACCCTA GATCTGGACG GTCCCCCACA 
GGCAACACCC CCCCAGTGAT TGACAGTGTT TCAGAGAAGG GAAGTTCAAG CATTAAAGAT 
TCAAAAGACA CCCATGGGAA ACAGAGTGTG GGCAGTGGCA GTCCTGTGCA AACCGTGGGT 
CTGGAAACCC GCCTCAACTC CTTTGTTCAG GTAGAGGCCC CAGAACAGAA AGGAACTGAG 
GCAAAACCAG GACAGAGTAA CCCAGTCTCT ATAGCAGAGA CTGCTGAGAC GTGTATAGCA 
GAGCGTACCC CTTTCAGTTC CAGTAGCTCC AGCAAGCACA GCTCACCTAG CGGGACTGTT 
GCTGCCAGAG TGACACCTTT TAATTACAAC CCTAGCCCTA GGAAGAGCAG CGCAGACAGC 
ACTTCAGCCC GGCCGTCTCA GATCCCTACG CCAGTGAGCA CCAACACGAA GAAGAGAGAT 
TCGAAGACTG ACAGCACAGA ATCCAGTGGA GCCCAAAGTC CTAAACGCCA TTCCGGGTCT 
TACCTCGTGA CGTCTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250288.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106148.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X19, mRNA.

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CloneID	OMu87327
Clone ID Related Accession (Same CDS sequence)	XM_030250289.1 , XM_030250288.1
Accession Version	XM_030250288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGTC CAGCTACACG TGTGGATCAC GAAACAGCCA GTGTTTTGAG TTCTAGCGGC 
ACGCACTCTG CTCCTCGAAG GTTGACAAGT CATCTGGGGA CAAAGGTGGA AATGGTGTAT 
TCCTTGTTGT CAATGCTTGG TACTCATGAT AAGGACGATA TGTCACGAAC TTTGCTAGCT 
ATGTCCAGCT CCCAAGACAG CTGTATATCC ATGCGGCAGT CTGGATGTCT TCCTCTCCTC 
ATCCAGCTTT TACATGGCAA TGACAAAGAC TCTGTATTGT TGGGAAATTC CCGGGGCAGT 
AAAGAGGCTC GGGCCAGGGC CAGTGCAGCA CTCCACAACA TCATTCACTC ACAGCCTGAT 
GACAAGAGAG GCAGGCGTGA AATCCGAGTC CTTCATCTTT TGGAACAGAT ACGAGCTTAC 
TGTGAAACCT GTTGGGAGTG GCAGGAAGCC CACGAACAAG GCATGGACCA GGACAAAAAC 
CCAATGCCAG CTCCTGTTGA GCATCAGATC TGTCCTGCTG TGTGTGTTCT AATGAAGCTT 
TCATTTGATG AAGAGCATAG GCATGCAATG AATGAACTTG GTAGGAAGGC TACCCGGGGC 
ATTTCATCCC AGGAGCTAGG TCAGGGGCTT TCAGGGGGAC TGCAGGCCAT TGCAGAGTTA 
TTGCAGGTGG ACTGTGAGAT GTATGGGCTT ACTAATGACC ACTACAGTGT TACTTTAAGA 
CGGTATGCTG GAATGGCTTT GACAAACTTG ACCTTTGGAG ATGTTGCCAA CAAGGCTACG 
CTGTGTTCTA TGAAAGGCTG CATGAGAGCA CTTGTGGCCC AGTTAAAATC TGAGAGTGAA 
GACTTACAGC AGGTTATTGC AAGTGTTTTG AGGAATTTGT CTTGGCGAGC AGATGTAAAT 
AGCAAAAAGA CGTTGAGAGA AGTTGGAAGT GTGAAAGCAT TGATGGAATG TGCTTTGGAA 
GTTAAAAAGG AATCAACCCT CAAAAGCGTT TTGAGTGCCT TATGGAACCT GTCTGCACAC 
TGCACTGAGA ATAAGGCTGA CATCTGTGCT GTGGATGGAG CACTGGCATT TCTGGTTGGC 
ACCCTCACTT ACCGGAGCCA GACAAATACT TTAGCCATTA TTGAAAGTGG AGGTGGGATA 
TTACGGAATG TGTCCAGCTT GATAGCTACA AACGAAGACC ACAGGCAAAT CCTAAGAGAG 
AACAATTGCC TACAAACTTT ATTACAGCAC TTGAAATCTC ACAGCTTGAC AATAGTCAGT 
AATGCATGTG GAACTTTGTG GAATCTCTCA GCAAGAAATC CTAAAGACCA GGAAGCCTTG 
TGGGACATGG GGGCAGTGAG CATGCTCAAG AACCTCATTC ATTCCAAGCA CAAAATGATT 
GCCATGGGAA GTGCAGCAGC TTTAAGGAAT CTCATGGCAA ACAGACCTGC AAAGTATAAG 
GATGCCAATA TCATGTCTCC CGGCTCAAGT CTGCCATCCC TTCACGTTAG GAAACAGAAA 
GCTCTAGAAG CTGAGCTAGA TGCTCAGCAT TTATCAGAAA CCTTCGACAA CATTGACAAC 
CTAAGTCCCA AGGCCTCTCA CCGGAGTAAG CAGAGACACA AGCAGAATCT TTATGGTGAC 
TATGCTTTTG ACGCCAATCG ACATGATGAT AGTAGGTCAG ACAATTTCAA TACTGGAAAC 
ATGACTGTTC TTTCACCATA TTTAAATACT ACGGTATTGC CCAGCTCTTC TTCCTCAAGG 
GGAAGTTTAG ACAGTTCTCG TTCTGAGAAA GACAGAAGTT TGGAGAGAGA GCGAGGTATT 
GGCCTCAGTG CTTACCATCC AACAACAGAA AATGCAGGAA CCTCATCAAA ACGAGGTCTG 
CAGATCACTA CCACTGCAGC CCAGATAGCC AAAGTTATGG AAGAAGTATC AGCCATTCAT 
ACCTCCCAGG ACGACAGAAG TTCTGCTTCT ACCACCGAGT TCCATTGTGT GGCAGACGAC 
AGGAGTGCGG CACGAAGAAG CTCTGCCTCC CACACACACT CAAACACATA CAACTTCACT 
AAGTCGGAAA ATTCAAATAG GACATGCTCT ATGCCTTATG CCAAAGTGGA ATATAAACGA 
TCTTCAAATG ACAGTTTAAA TAGTGTCACT AGTAGTGATG GATATGGTAA AAGAGGCCAA 
ATGAAACCCT CAGTTGAATC CTATTCTGAA GATGATGAAA GTAAATTTTG CAGTTATGGT 
CAGTATCCAG CTGACCTAGC CCATAAGATA CACAGTGCAA ATCATATGGA TGATAATGAT 
GGAGAACTGG ATACACCAAT AAATTACAGT CTTAAATATT CAGATGAGCA GTTGAACTCA 
GGAAGGCAGA GTCCCTCACA GAATGAAAGG TGGGCAAGAC CAAAGCATGT GATAGAAGAT 
GAAATAAAGC AAAACGAGCA AAGACAAGCA AGAAGCCAGA ACACCAGTTA TCCTGTCTAT 
TCTGAGAATA CCGATGACAA ACACCTCAAA TTCCAACCAC ATTTTGGACA ACAAGAATGT 
GTTTCCCCAT ATAGGTCAAG GGGAACCAGT GGTTCAGAAA CAAATCGAAT GGGTTCTAGT 
CATGCAATTA ATCAAAATGT AAACCAGTCT CTGTGTCAGG AAGATGATTA TGAAGATGAT 
AAACCTACCA ACTACAGTGA ACGTTATTCT GAGGAAGAAC AACATGAAGA AGAAGAAGAG 
AGACCGACAA ATTATAGCAT AAAATATAAT GAAGAGAAAC ATCATGTGGA TCAGCCTATT 
GATTATAGTT TAAAATATGC CACTGACATT TCTTCCTCAC AAAAACCATC ATTTTCATTC 
TCAAAGAATT CATCAGCACA AAGCACTAAA CCTGAACATC TCTCTCCAAG CAGCGAGAAT 
ACAGCTGTAC CTCCATCTAA TGCCAAAAGG CAGAATCAGC TGCGTCCAAG TTCAGCACAA 
AGAAATGGCC AGACTCAAAA AGGCACTACT TGCAAAGTCC CCTCCATCAA CCAAGAAACA 
ATACAGACTT ACTGCGTAGA AGACACCCCA ATATGTTTTT CAAGGTGCAG TTCATTATCA 
TCACTGTCAT CAGCTGACGA TGAAATAGGA TGTGATCAGA CAACACAGGA AGCAGATTCT 
GCTAATACTC TGCAGACAGC AGAAGTAAAA GAGAATGATG TAACTCGGTC AGCTGAAGAT 
CCTGCAACTG AAGTTCCAGC AGTGTCCCAG AATGCTAGAG CCAAACCCAG CCGACTCCAG 
GCTTCTGGCT TATCTTCAGA ATCAACCAGG CATAATAAAG CTGTTGAGTT TTCTTCAGGA 
GCCAAGTCTC CCTCCAAAAG TGGTGCTCAG ACACCCAAAA GTCCCCCAGA ACACTATGTC 
CAGGAGACTC CGCTCGTATT CAGCAGGTGT ACTTCTGTCA GCTCCCTTGA CAGTTTTGAG 
AGTCGCTCCA TTGCCAGCTC TGTTCAGAGT GAGCCATGTA GTGGAATGGT GAGTGGCATC 
ATAAGCCCCA GTGACCTTCC AGATAGTCCT GGGCAGACCA TGCCACCAAG CAGAAGCAAA 
ACCCCTCCAC CTCCTCCACA GACAGTGCAG GCCAAGAGAG AGGTGCCAAA AAGTAAAGTC 
CCTGCTGCTG AGAAGAGAGA GAGTGGGCCT AAGCAGACTG CTGTAAATGC TGCCGTGCAG 
AGGGTGCAGG TCCTTCCAGA CGTGGATACT TTGTTACACT TCGCCACAGA AAGTACTCCA 
GACGGGTTTT CTTGTTCCTC CAGCCTAAGT GCTCTGAGCC TGGATGAGCC ATTTATACAG 
AAAGATGTAG AATTAAGAAT CATGCCTCCA GTTCAGGAAA ACGACAATGG GAATGAAACT 
GAATCAGAAC AGCCTGAGGA ATCAAATGAA AACCAGGATA AAGAGGTAGA AAAGCCTGAC 
TCTGAAAAAG ACTTATTAGA TGATTCTGAT GACGATGATA TTGAAATATT AGAAGAATGT 
ATTATTTCAG CCATGCCAAC AAAGTCATCA CGCAAAGCCA AAAAACTAGC CCAGACTGCT 
TCAAAATTAC CTCCACCTGT GGCAAGGAAA CCAAGTCAGC TACCTGTGTA TAAACTTCTG 
CCAGCACAGA ATAGGCTGCA GGCACAAAAA CATGTTAGCT TTACACCAGG GGATGATGTG 
CCCCGGGTGT ACTGTGTAGA AGGGACACCT ATAAACTTTT CCACAGCAAC GTCTCTAAGT 
GATCTGACAA TAGAGTCCCC TCCAAATGAA TTGGCTACTG GAGATGGGGT CAGAGCGGGT 
ATACAGTCAG GTGAATTTGA AAAACGAGAT ACCATTCCTA CAGAAGGCAG AAGTACAGAT 
GATGCTCAGC GAGGAAAAAT CTCATCTATA GTTACACCAG ACCTGGATGA CAACAAAGCA 
GAGGAAGGAG ATATTCTTGC AGAATGTATC AATTCTGCTA TGCCCAAAGG AAAAAGCCAC 
AAGCCTTTCC GAGTGAAAAA GATAATGGAC CAAGTCCAAC AAGCATCCTC GACTTCATCT 
GGAGCTAACA AAAATCAAGT AGACACTAAG AAAAAGAAGC CTACTTCACC AGTAAAGCCC 
ATGCCACAAA ATACTGAATA TAGAACGCGT GTGAGAAAGA ATACAGACTC AAAAGTTAAT 
GTAAATACTG AAGAAACTTT CTCAGACAAC AAAGACTCAA AGAAACCAAG CTTACAAACC 
AATGCCAAGG CCTTCAATGA AAAGCTACCT AACAATGAAG ACAGAGTGCG GGGGAGCTTC 
GCCTTGGACT CACCGCATCA CTACACCCCT ATTGAGGGGA CGCCGTACTG CTTTTCCCGA 
AATGACTCCT TGAGTTCTCT GGATTTTGAT GATGACGATG TTGACCTTTC CAGGGAAAAG 
GCCGAGTTAA GAAAGGGCAA AGAAAGCAAG GATTCCGAAG CCAAAGTTAC CTGCCGCCCA 
GAACCAAACT CAAGCCAGCA GGCAGCTAGT AAGTCACAAG CCAGTATAAA ACATCCAGCA 
AACAGAGCAC AGTCCAAACC AGTGCTGCAG AAACAGCCCA CTTTCCCCCA GTCCTCCAAA 
GACGGACCAG ATAGAGGGGC AGCAACTGAC GAAAAACTGC AGAATTTTGC TATTGAAAAT 
ACTCCAGTTT GCTTTTCTCG AAATTCCTCT CTGAGTTCCC TTAGTGACAT TGACCAGGAA 
AACAACAATA ACAAAGAAAG TGAACCAATC AAAGAAGCTG AACCTGCCAA CTCACAAGGA 
GAGCCCAGTA AGCCTCAGGC ATCCGGGTAT GCTCCCAAGT CCTTCCACGT CGAAGACACC 
CCTGTCTGTT TCTCAAGAAA CAGCTCTCTC AGTTCTCTTA GCATTGACTC TGAGGACGAC 
CTGTTACAGG AGTGTATAAG TTCTGCCATG CCAAAAAAGA AAAGGCCTTC AAGACTCAAG 
AGTGAGAGCG AAAAGCAGAG CCCTAGAAAA GTGGGTGGCA TATTAGCTGA AGACCTGACG 
CTTGATTTGA AAGATCTACA GAGGCCAGAT TCAGAACACG CTTTCTCCCC CGACTCAGAA 
AATTTTGACT GGAAAGCTAT TCAGGAAGGC GCAAACTCCA TAGTAAGTAG TTTGCACCAA 
GCTGCTGCAG CCGCCGCGTG CTTATCTAGA CAAGCGTCAT CCGACTCAGA TTCCATTCTG 
TCACTAAAGT CCGGCATTTC TCTGGGATCG CCTTTTCATC TTACACCTGA TCAAGAGGAA 
AAGCCATTCA CAAGCAATAA AGGCCCAAGA ATTCTCAAAC CTGGAGAGAA AAGCACATTA 
GAAGCAAAAA AAATAGAATC TGAAAACAAA GGAATCAAAG GCGGGAAAAA GGTTTATAAA 
AGCTTGATTA CGGGAAAGAT TCGCTCCAAT TCAGAAATTT CCAGCCAAAT GAAACAACCC 
CTCCCGACAA ACATGCCTTC AATCTCAAGA GGCAGGACGA TGATTCACAT CCCAGGGCTT 
CGGAATAGCT CCTCTAGTAC AAGCCCTGTC TCTAAGAAAG GCCCACCCCT CAAGACTCCA 
GCCTCTAAAA GCCCCAGTGA AGGGCCGGGA GCTACCACTT CTCCTCGAGG AACTAAGCCA 
GCAGGAAAGT CAGAGCTTAG CCCTATCACC AGGCAAACTT CCCAAATCAG TGGGTCAAAT 
AAGGGGTCTT CTAGATCAGG ATCTAGAGAC TCCACTCCCT CAAGACCTAC ACAGCAACCA 
TTAAGTAGGC CAATGCAGTC TCCAGGGCGA AACTCAATTT CCCCTGGTAG AAATGGAATA 
AGCCCTCCTA ACAAACTGTC TCAGCTGCCC AGAACATCAT CTCCCAGTAC TGCTTCAACT 
AAGTCCTCCG GTTCTGGGAA AATGTCATAT ACATCCCCAG GTAGACAGCT GAGCCAACAA 
AATCTTACCA AACAAGCAAG TTTATCCAAG AATGCCAGCA GTATCCCCAG AAGTGAGTCG 
GCATCTAAAG GACTGAATCA GATGAGTAAC GGCAATGGGT CAAATAAAAA GGTAGAACTT 
TCTAGAATGT CTTCAACTAA ATCAAGTGGA AGTGAATCAG ACAGATCAGA AAGGCCTGCA 
TTAGTACGCC AGTCTACTTT CATCAAAGAA GCCCCAAGCC CAACCCTGAG GAGGAAACTG 
GAGGAATCTG CCTCATTTGA ATCCCTTTCT CCATCTTCTA GACCAGATTC TCCCACCAGG 
TCGCAGGCAC AGACCCCAGT TTTAAGCCCT TCCCTTCCTG ATATGTCTCT GTCCACACAT 
CCATCTGTTC AGGCAGGTGG GTGGCGAAAG CTCCCGCCTA ATCTCAGCCC CACTATCGAG 
TATAATGACG GAAGGCCCAC AAAACGGCAT GATATTGCAC GCTCCCATTC TGAAAGTCCT 
TCCAGACTAC CAATCAACCG GGCGGGAACC TGGAAGCGTG AACACAGCAA ACATTCCTCG 
TCCCTTCCTC GAGTGAGTAC TTGGAGAAGA ACTGGAAGCT CATCTTCTAT TCTTTCTGCT 
TCATCAGAGT CCAGTGAAAA AGCAAAAAGT GAGGATGAAA GGCATGTGAG CTCCATGCCA 
GCACCCAGAC AGATGAAGGA AAACCAGGTG CCCACCAAAG GAACATGGAG GAAAATCAAG 
GAAAGTGACA TTTCTCCCAC AGGCATGGCT TCTCAGAGCG CTTCCTCAGG TGCTGCCAGT 
GGTGCTGAAT CCAAGCCTCT GATCTATCAG ATGGCACCTC CTGTCTCTAA AACAGAGGAT 
GTTTGGGTGA GAATTGAGGA CTGCCCCATT AACAACCCTA GATCTGGACG GTCCCCCACA 
GGCAACACCC CCCCAGTGAT TGACAGTGTT TCAGAGAAGG GAAGTTCAAG CATTAAAGAT 
TCAAAAGACA CCCATGGGAA ACAGAGTGTG GGCAGTGGCA GTCCTGTGCA AACCGTGGGT 
CTGGAAACCC GCCTCAACTC CTTTGTTCAG GTAGAGGCCC CAGAACAGAA AGGAACTGAG 
GCAAAACCAG GACAGAGTAA CCCAGTCTCT ATAGCAGAGA CTGCTGAGAC GTGTATAGCA 
GAGCGTACCC CTTTCAGTTC CAGTAGCTCC AGCAAGCACA GCTCACCTAG CGGGACTGTT 
GCTGCCAGAG TGACACCTTT TAATTACAAC CCTAGCCCTA GGAAGAGCAG CGCAGACAGC 
ACTTCAGCCC GGCCGTCTCA GATCCCTACG CCAGTGAGCA CCAACACGAA GAAGAGAGAT 
TCGAAGACTG ACAGCACAGA ATCCAGTGGA GCCCAAAGTC CTAAACGCCA TTCCGGGTCT 
TACCTCGTGA CGTCTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106148.1
CDS	725..9025
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAESPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250288.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250288.1

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGTC CAGCTACACG TGTGGATCAC GAAACAGCCA GTGTTTTGAG TTCTAGCGGC 
ACGCACTCTG CTCCTCGAAG GTTGACAAGT CATCTGGGGA CAAAGGTGGA AATGGTGTAT 
TCCTTGTTGT CAATGCTTGG TACTCATGAT AAGGACGATA TGTCACGAAC TTTGCTAGCT 
ATGTCCAGCT CCCAAGACAG CTGTATATCC ATGCGGCAGT CTGGATGTCT TCCTCTCCTC 
ATCCAGCTTT TACATGGCAA TGACAAAGAC TCTGTATTGT TGGGAAATTC CCGGGGCAGT 
AAAGAGGCTC GGGCCAGGGC CAGTGCAGCA CTCCACAACA TCATTCACTC ACAGCCTGAT 
GACAAGAGAG GCAGGCGTGA AATCCGAGTC CTTCATCTTT TGGAACAGAT ACGAGCTTAC 
TGTGAAACCT GTTGGGAGTG GCAGGAAGCC CACGAACAAG GCATGGACCA GGACAAAAAC 
CCAATGCCAG CTCCTGTTGA GCATCAGATC TGTCCTGCTG TGTGTGTTCT AATGAAGCTT 
TCATTTGATG AAGAGCATAG GCATGCAATG AATGAACTTG GTAGGAAGGC TACCCGGGGC 
ATTTCATCCC AGGAGCTAGG TCAGGGGCTT TCAGGGGGAC TGCAGGCCAT TGCAGAGTTA 
TTGCAGGTGG ACTGTGAGAT GTATGGGCTT ACTAATGACC ACTACAGTGT TACTTTAAGA 
CGGTATGCTG GAATGGCTTT GACAAACTTG ACCTTTGGAG ATGTTGCCAA CAAGGCTACG 
CTGTGTTCTA TGAAAGGCTG CATGAGAGCA CTTGTGGCCC AGTTAAAATC TGAGAGTGAA 
GACTTACAGC AGGTTATTGC AAGTGTTTTG AGGAATTTGT CTTGGCGAGC AGATGTAAAT 
AGCAAAAAGA CGTTGAGAGA AGTTGGAAGT GTGAAAGCAT TGATGGAATG TGCTTTGGAA 
GTTAAAAAGG AATCAACCCT CAAAAGCGTT TTGAGTGCCT TATGGAACCT GTCTGCACAC 
TGCACTGAGA ATAAGGCTGA CATCTGTGCT GTGGATGGAG CACTGGCATT TCTGGTTGGC 
ACCCTCACTT ACCGGAGCCA GACAAATACT TTAGCCATTA TTGAAAGTGG AGGTGGGATA 
TTACGGAATG TGTCCAGCTT GATAGCTACA AACGAAGACC ACAGGCAAAT CCTAAGAGAG 
AACAATTGCC TACAAACTTT ATTACAGCAC TTGAAATCTC ACAGCTTGAC AATAGTCAGT 
AATGCATGTG GAACTTTGTG GAATCTCTCA GCAAGAAATC CTAAAGACCA GGAAGCCTTG 
TGGGACATGG GGGCAGTGAG CATGCTCAAG AACCTCATTC ATTCCAAGCA CAAAATGATT 
GCCATGGGAA GTGCAGCAGC TTTAAGGAAT CTCATGGCAA ACAGACCTGC AAAGTATAAG 
GATGCCAATA TCATGTCTCC CGGCTCAAGT CTGCCATCCC TTCACGTTAG GAAACAGAAA 
GCTCTAGAAG CTGAGCTAGA TGCTCAGCAT TTATCAGAAA CCTTCGACAA CATTGACAAC 
CTAAGTCCCA AGGCCTCTCA CCGGAGTAAG CAGAGACACA AGCAGAATCT TTATGGTGAC 
TATGCTTTTG ACGCCAATCG ACATGATGAT AGTAGGTCAG ACAATTTCAA TACTGGAAAC 
ATGACTGTTC TTTCACCATA TTTAAATACT ACGGTATTGC CCAGCTCTTC TTCCTCAAGG 
GGAAGTTTAG ACAGTTCTCG TTCTGAGAAA GACAGAAGTT TGGAGAGAGA GCGAGGTATT 
GGCCTCAGTG CTTACCATCC AACAACAGAA AATGCAGGAA CCTCATCAAA ACGAGGTCTG 
CAGATCACTA CCACTGCAGC CCAGATAGCC AAAGTTATGG AAGAAGTATC AGCCATTCAT 
ACCTCCCAGG ACGACAGAAG TTCTGCTTCT ACCACCGAGT TCCATTGTGT GGCAGACGAC 
AGGAGTGCGG CACGAAGAAG CTCTGCCTCC CACACACACT CAAACACATA CAACTTCACT 
AAGTCGGAAA ATTCAAATAG GACATGCTCT ATGCCTTATG CCAAAGTGGA ATATAAACGA 
TCTTCAAATG ACAGTTTAAA TAGTGTCACT AGTAGTGATG GATATGGTAA AAGAGGCCAA 
ATGAAACCCT CAGTTGAATC CTATTCTGAA GATGATGAAA GTAAATTTTG CAGTTATGGT 
CAGTATCCAG CTGACCTAGC CCATAAGATA CACAGTGCAA ATCATATGGA TGATAATGAT 
GGAGAACTGG ATACACCAAT AAATTACAGT CTTAAATATT CAGATGAGCA GTTGAACTCA 
GGAAGGCAGA GTCCCTCACA GAATGAAAGG TGGGCAAGAC CAAAGCATGT GATAGAAGAT 
GAAATAAAGC AAAACGAGCA AAGACAAGCA AGAAGCCAGA ACACCAGTTA TCCTGTCTAT 
TCTGAGAATA CCGATGACAA ACACCTCAAA TTCCAACCAC ATTTTGGACA ACAAGAATGT 
GTTTCCCCAT ATAGGTCAAG GGGAACCAGT GGTTCAGAAA CAAATCGAAT GGGTTCTAGT 
CATGCAATTA ATCAAAATGT AAACCAGTCT CTGTGTCAGG AAGATGATTA TGAAGATGAT 
AAACCTACCA ACTACAGTGA ACGTTATTCT GAGGAAGAAC AACATGAAGA AGAAGAAGAG 
AGACCGACAA ATTATAGCAT AAAATATAAT GAAGAGAAAC ATCATGTGGA TCAGCCTATT 
GATTATAGTT TAAAATATGC CACTGACATT TCTTCCTCAC AAAAACCATC ATTTTCATTC 
TCAAAGAATT CATCAGCACA AAGCACTAAA CCTGAACATC TCTCTCCAAG CAGCGAGAAT 
ACAGCTGTAC CTCCATCTAA TGCCAAAAGG CAGAATCAGC TGCGTCCAAG TTCAGCACAA 
AGAAATGGCC AGACTCAAAA AGGCACTACT TGCAAAGTCC CCTCCATCAA CCAAGAAACA 
ATACAGACTT ACTGCGTAGA AGACACCCCA ATATGTTTTT CAAGGTGCAG TTCATTATCA 
TCACTGTCAT CAGCTGACGA TGAAATAGGA TGTGATCAGA CAACACAGGA AGCAGATTCT 
GCTAATACTC TGCAGACAGC AGAAGTAAAA GAGAATGATG TAACTCGGTC AGCTGAAGAT 
CCTGCAACTG AAGTTCCAGC AGTGTCCCAG AATGCTAGAG CCAAACCCAG CCGACTCCAG 
GCTTCTGGCT TATCTTCAGA ATCAACCAGG CATAATAAAG CTGTTGAGTT TTCTTCAGGA 
GCCAAGTCTC CCTCCAAAAG TGGTGCTCAG ACACCCAAAA GTCCCCCAGA ACACTATGTC 
CAGGAGACTC CGCTCGTATT CAGCAGGTGT ACTTCTGTCA GCTCCCTTGA CAGTTTTGAG 
AGTCGCTCCA TTGCCAGCTC TGTTCAGAGT GAGCCATGTA GTGGAATGGT GAGTGGCATC 
ATAAGCCCCA GTGACCTTCC AGATAGTCCT GGGCAGACCA TGCCACCAAG CAGAAGCAAA 
ACCCCTCCAC CTCCTCCACA GACAGTGCAG GCCAAGAGAG AGGTGCCAAA AAGTAAAGTC 
CCTGCTGCTG AGAAGAGAGA GAGTGGGCCT AAGCAGACTG CTGTAAATGC TGCCGTGCAG 
AGGGTGCAGG TCCTTCCAGA CGTGGATACT TTGTTACACT TCGCCACAGA AAGTACTCCA 
GACGGGTTTT CTTGTTCCTC CAGCCTAAGT GCTCTGAGCC TGGATGAGCC ATTTATACAG 
AAAGATGTAG AATTAAGAAT CATGCCTCCA GTTCAGGAAA ACGACAATGG GAATGAAACT 
GAATCAGAAC AGCCTGAGGA ATCAAATGAA AACCAGGATA AAGAGGTAGA AAAGCCTGAC 
TCTGAAAAAG ACTTATTAGA TGATTCTGAT GACGATGATA TTGAAATATT AGAAGAATGT 
ATTATTTCAG CCATGCCAAC AAAGTCATCA CGCAAAGCCA AAAAACTAGC CCAGACTGCT 
TCAAAATTAC CTCCACCTGT GGCAAGGAAA CCAAGTCAGC TACCTGTGTA TAAACTTCTG 
CCAGCACAGA ATAGGCTGCA GGCACAAAAA CATGTTAGCT TTACACCAGG GGATGATGTG 
CCCCGGGTGT ACTGTGTAGA AGGGACACCT ATAAACTTTT CCACAGCAAC GTCTCTAAGT 
GATCTGACAA TAGAGTCCCC TCCAAATGAA TTGGCTACTG GAGATGGGGT CAGAGCGGGT 
ATACAGTCAG GTGAATTTGA AAAACGAGAT ACCATTCCTA CAGAAGGCAG AAGTACAGAT 
GATGCTCAGC GAGGAAAAAT CTCATCTATA GTTACACCAG ACCTGGATGA CAACAAAGCA 
GAGGAAGGAG ATATTCTTGC AGAATGTATC AATTCTGCTA TGCCCAAAGG AAAAAGCCAC 
AAGCCTTTCC GAGTGAAAAA GATAATGGAC CAAGTCCAAC AAGCATCCTC GACTTCATCT 
GGAGCTAACA AAAATCAAGT AGACACTAAG AAAAAGAAGC CTACTTCACC AGTAAAGCCC 
ATGCCACAAA ATACTGAATA TAGAACGCGT GTGAGAAAGA ATACAGACTC AAAAGTTAAT 
GTAAATACTG AAGAAACTTT CTCAGACAAC AAAGACTCAA AGAAACCAAG CTTACAAACC 
AATGCCAAGG CCTTCAATGA AAAGCTACCT AACAATGAAG ACAGAGTGCG GGGGAGCTTC 
GCCTTGGACT CACCGCATCA CTACACCCCT ATTGAGGGGA CGCCGTACTG CTTTTCCCGA 
AATGACTCCT TGAGTTCTCT GGATTTTGAT GATGACGATG TTGACCTTTC CAGGGAAAAG 
GCCGAGTTAA GAAAGGGCAA AGAAAGCAAG GATTCCGAAG CCAAAGTTAC CTGCCGCCCA 
GAACCAAACT CAAGCCAGCA GGCAGCTAGT AAGTCACAAG CCAGTATAAA ACATCCAGCA 
AACAGAGCAC AGTCCAAACC AGTGCTGCAG AAACAGCCCA CTTTCCCCCA GTCCTCCAAA 
GACGGACCAG ATAGAGGGGC AGCAACTGAC GAAAAACTGC AGAATTTTGC TATTGAAAAT 
ACTCCAGTTT GCTTTTCTCG AAATTCCTCT CTGAGTTCCC TTAGTGACAT TGACCAGGAA 
AACAACAATA ACAAAGAAAG TGAACCAATC AAAGAAGCTG AACCTGCCAA CTCACAAGGA 
GAGCCCAGTA AGCCTCAGGC ATCCGGGTAT GCTCCCAAGT CCTTCCACGT CGAAGACACC 
CCTGTCTGTT TCTCAAGAAA CAGCTCTCTC AGTTCTCTTA GCATTGACTC TGAGGACGAC 
CTGTTACAGG AGTGTATAAG TTCTGCCATG CCAAAAAAGA AAAGGCCTTC AAGACTCAAG 
AGTGAGAGCG AAAAGCAGAG CCCTAGAAAA GTGGGTGGCA TATTAGCTGA AGACCTGACG 
CTTGATTTGA AAGATCTACA GAGGCCAGAT TCAGAACACG CTTTCTCCCC CGACTCAGAA 
AATTTTGACT GGAAAGCTAT TCAGGAAGGC GCAAACTCCA TAGTAAGTAG TTTGCACCAA 
GCTGCTGCAG CCGCCGCGTG CTTATCTAGA CAAGCGTCAT CCGACTCAGA TTCCATTCTG 
TCACTAAAGT CCGGCATTTC TCTGGGATCG CCTTTTCATC TTACACCTGA TCAAGAGGAA 
AAGCCATTCA CAAGCAATAA AGGCCCAAGA ATTCTCAAAC CTGGAGAGAA AAGCACATTA 
GAAGCAAAAA AAATAGAATC TGAAAACAAA GGAATCAAAG GCGGGAAAAA GGTTTATAAA 
AGCTTGATTA CGGGAAAGAT TCGCTCCAAT TCAGAAATTT CCAGCCAAAT GAAACAACCC 
CTCCCGACAA ACATGCCTTC AATCTCAAGA GGCAGGACGA TGATTCACAT CCCAGGGCTT 
CGGAATAGCT CCTCTAGTAC AAGCCCTGTC TCTAAGAAAG GCCCACCCCT CAAGACTCCA 
GCCTCTAAAA GCCCCAGTGA AGGGCCGGGA GCTACCACTT CTCCTCGAGG AACTAAGCCA 
GCAGGAAAGT CAGAGCTTAG CCCTATCACC AGGCAAACTT CCCAAATCAG TGGGTCAAAT 
AAGGGGTCTT CTAGATCAGG ATCTAGAGAC TCCACTCCCT CAAGACCTAC ACAGCAACCA 
TTAAGTAGGC CAATGCAGTC TCCAGGGCGA AACTCAATTT CCCCTGGTAG AAATGGAATA 
AGCCCTCCTA ACAAACTGTC TCAGCTGCCC AGAACATCAT CTCCCAGTAC TGCTTCAACT 
AAGTCCTCCG GTTCTGGGAA AATGTCATAT ACATCCCCAG GTAGACAGCT GAGCCAACAA 
AATCTTACCA AACAAGCAAG TTTATCCAAG AATGCCAGCA GTATCCCCAG AAGTGAGTCG 
GCATCTAAAG GACTGAATCA GATGAGTAAC GGCAATGGGT CAAATAAAAA GGTAGAACTT 
TCTAGAATGT CTTCAACTAA ATCAAGTGGA AGTGAATCAG ACAGATCAGA AAGGCCTGCA 
TTAGTACGCC AGTCTACTTT CATCAAAGAA GCCCCAAGCC CAACCCTGAG GAGGAAACTG 
GAGGAATCTG CCTCATTTGA ATCCCTTTCT CCATCTTCTA GACCAGATTC TCCCACCAGG 
TCGCAGGCAC AGACCCCAGT TTTAAGCCCT TCCCTTCCTG ATATGTCTCT GTCCACACAT 
CCATCTGTTC AGGCAGGTGG GTGGCGAAAG CTCCCGCCTA ATCTCAGCCC CACTATCGAG 
TATAATGACG GAAGGCCCAC AAAACGGCAT GATATTGCAC GCTCCCATTC TGAAAGTCCT 
TCCAGACTAC CAATCAACCG GGCGGGAACC TGGAAGCGTG AACACAGCAA ACATTCCTCG 
TCCCTTCCTC GAGTGAGTAC TTGGAGAAGA ACTGGAAGCT CATCTTCTAT TCTTTCTGCT 
TCATCAGAGT CCAGTGAAAA AGCAAAAAGT GAGGATGAAA GGCATGTGAG CTCCATGCCA 
GCACCCAGAC AGATGAAGGA AAACCAGGTG CCCACCAAAG GAACATGGAG GAAAATCAAG 
GAAAGTGACA TTTCTCCCAC AGGCATGGCT TCTCAGAGCG CTTCCTCAGG TGCTGCCAGT 
GGTGCTGAAT CCAAGCCTCT GATCTATCAG ATGGCACCTC CTGTCTCTAA AACAGAGGAT 
GTTTGGGTGA GAATTGAGGA CTGCCCCATT AACAACCCTA GATCTGGACG GTCCCCCACA 
GGCAACACCC CCCCAGTGAT TGACAGTGTT TCAGAGAAGG GAAGTTCAAG CATTAAAGAT 
TCAAAAGACA CCCATGGGAA ACAGAGTGTG GGCAGTGGCA GTCCTGTGCA AACCGTGGGT 
CTGGAAACCC GCCTCAACTC CTTTGTTCAG GTAGAGGCCC CAGAACAGAA AGGAACTGAG 
GCAAAACCAG GACAGAGTAA CCCAGTCTCT ATAGCAGAGA CTGCTGAGAC GTGTATAGCA 
GAGCGTACCC CTTTCAGTTC CAGTAGCTCC AGCAAGCACA GCTCACCTAG CGGGACTGTT 
GCTGCCAGAG TGACACCTTT TAATTACAAC CCTAGCCCTA GGAAGAGCAG CGCAGACAGC 
ACTTCAGCCC GGCCGTCTCA GATCCCTACG CCAGTGAGCA CCAACACGAA GAAGAGAGAT 
TCGAAGACTG ACAGCACAGA ATCCAGTGGA GCCCAAAGTC CTAAACGCCA TTCCGGGTCT 
TACCTCGTGA CGTCTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250290.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106150.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X21, mRNA.

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CloneID	OMu87328
Clone ID Related Accession (Same CDS sequence)	XM_030250290.1
Accession Version	XM_030250290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGATA CGAGCTTACT GTGAAACCTG TTGGGAGTGG 
CAGGAAGCCC ACGAACAAGG CATGGACCAG GACAAAAACC CAATGCCAGC TCCTGTTGAG 
CATCAGATCT GTCCTGCTGT GTGTGTTCTA ATGAAGCTTT CATTTGATGA AGAGCATAGG 
CATGCAATGA ATGAACTTGG TAGGAAGGCT ACCCGGGGCA TTTCATCCCA GGAGCTAGGT 
CAGGGGCTTT CAGGGGGACT GCAGGCCATT GCAGAGTTAT TGCAGGTGGA CTGTGAGATG 
TATGGGCTTA CTAATGACCA CTACAGTGTT ACTTTAAGAC GGTATGCTGG AATGGCTTTG 
ACAAACTTGA CCTTTGGAGA TGTTGCCAAC AAGGCTACGC TGTGTTCTAT GAAAGGCTGC 
ATGAGAGCAC TTGTGGCCCA GTTAAAATCT GAGAGTGAAG ACTTACAGCA GGTTATTGCA 
AGTGTTTTGA GGAATTTGTC TTGGCGAGCA GATGTAAATA GCAAAAAGAC GTTGAGAGAA 
GTTGGAAGTG TGAAAGCATT GATGGAATGT GCTTTGGAAG TTAAAAAGGA ATCAACCCTC 
AAAAGCGTTT TGAGTGCCTT ATGGAACCTG TCTGCACACT GCACTGAGAA TAAGGCTGAC 
ATCTGTGCTG TGGATGGAGC ACTGGCATTT CTGGTTGGCA CCCTCACTTA CCGGAGCCAG 
ACAAATACTT TAGCCATTAT TGAAAGTGGA GGTGGGATAT TACGGAATGT GTCCAGCTTG 
ATAGCTACAA ACGAAGACCA CAGGCAAATC CTAAGAGAGA ACAATTGCCT ACAAACTTTA 
TTACAGCACT TGAAATCTCA CAGCTTGACA ATAGTCAGTA ATGCATGTGG AACTTTGTGG 
AATCTCTCAG CAAGAAATCC TAAAGACCAG GAAGCCTTGT GGGACATGGG GGCAGTGAGC 
ATGCTCAAGA ACCTCATTCA TTCCAAGCAC AAAATGATTG CCATGGGAAG TGCAGCAGCT 
TTAAGGAATC TCATGGCAAA CAGACCTGCA AAGTATAAGG ATGCCAATAT CATGTCTCCC 
GGCTCAAGTC TGCCATCCCT TCACGTTAGG AAACAGAAAG CTCTAGAAGC TGAGCTAGAT 
GCTCAGCATT TATCAGAAAC CTTCGACAAC ATTGACAACC TAAGTCCCAA GGCCTCTCAC 
CGGAGTAAGC AGAGACACAA GCAGAATCTT TATGGTGACT ATGCTTTTGA CGCCAATCGA 
CATGATGATA GTAGGTCAGA CAATTTCAAT ACTGGAAACA TGACTGTTCT TTCACCATAT 
TTAAATACTA CGGTATTGCC CAGCTCTTCT TCCTCAAGGG GAAGTTTAGA CAGTTCTCGT 
TCTGAGAAAG ACAGAAGTTT GGAGAGAGAG CGAGGTATTG GCCTCAGTGC TTACCATCCA 
ACAACAGAAA ATGCAGGAAC CTCATCAAAA CGAGGTCTGC AGATCACTAC CACTGCAGCC 
CAGATAGCCA AAGTTATGGA AGAAGTATCA GCCATTCATA CCTCCCAGGA CGACAGAAGT 
TCTGCTTCTA CCACCGAGTT CCATTGTGTG GCAGACGACA GGAGTGCGGC ACGAAGAAGC 
TCTGCCTCCC ACACACACTC AAACACATAC AACTTCACTA AGTCGGAAAA TTCAAATAGG 
ACATGCTCTA TGCCTTATGC CAAAGTGGAA TATAAACGAT CTTCAAATGA CAGTTTAAAT 
AGTGTCACTA GTAGTGATGG ATATGGTAAA AGAGGCCAAA TGAAACCCTC AGTTGAATCC 
TATTCTGAAG ATGATGAAAG TAAATTTTGC AGTTATGGTC AGTATCCAGC TGACCTAGCC 
CATAAGATAC ACAGTGCAAA TCATATGGAT GATAATGATG GAGAACTGGA TACACCAATA 
AATTACAGTC TTAAATATTC AGATGAGCAG TTGAACTCAG GAAGGCAGAG TCCCTCACAG 
AATGAAAGGT GGGCAAGACC AAAGCATGTG ATAGAAGATG AAATAAAGCA AAACGAGCAA 
AGACAAGCAA GAAGCCAGAA CACCAGTTAT CCTGTCTATT CTGAGAATAC CGATGACAAA 
CACCTCAAAT TCCAACCACA TTTTGGACAA CAAGAATGTG TTTCCCCATA TAGGTCAAGG 
GGAACCAGTG GTTCAGAAAC AAATCGAATG GGTTCTAGTC ATGCAATTAA TCAAAATGTA 
AACCAGTCTC TGTGTCAGGA AGATGATTAT GAAGATGATA AACCTACCAA CTACAGTGAA 
CGTTATTCTG AGGAAGAACA ACATGAAGAA GAAGAAGAGA GACCGACAAA TTATAGCATA 
AAATATAATG AAGAGAAACA TCATGTGGAT CAGCCTATTG ATTATAGTTT AAAATATGCC 
ACTGACATTT CTTCCTCACA AAAACCATCA TTTTCATTCT CAAAGAATTC ATCAGCACAA 
AGCACTAAAC CTGAACATCT CTCTCCAAGC AGCGAGAATA CAGCTGTACC TCCATCTAAT 
GCCAAAAGGC AGAATCAGCT GCGTCCAAGT TCAGCACAAA GAAATGGCCA GACTCAAAAA 
GGCACTACTT GCAAAGTCCC CTCCATCAAC CAAGAAACAA TACAGACTTA CTGCGTAGAA 
GACACCCCAA TATGTTTTTC AAGGTGCAGT TCATTATCAT CACTGTCATC AGCTGACGAT 
GAAATAGGAT GTGATCAGAC AACACAGGAA GCAGATTCTG CTAATACTCT GCAGACAGCA 
GAAGTAAAAG AGAATGATGT AACTCGGTCA GCTGAAGATC CTGCAACTGA AGTTCCAGCA 
GTGTCCCAGA ATGCTAGAGC CAAACCCAGC CGACTCCAGG CTTCTGGCTT ATCTTCAGAA 
TCAACCAGGC ATAATAAAGC TGTTGAGTTT TCTTCAGGAG CCAAGTCTCC CTCCAAAAGT 
GGTGCTCAGA CACCCAAAAG TCCCCCAGAA CACTATGTCC AGGAGACTCC GCTCGTATTC 
AGCAGGTGTA CTTCTGTCAG CTCCCTTGAC AGTTTTGAGA GTCGCTCCAT TGCCAGCTCT 
GTTCAGAGTG AGCCATGTAG TGGAATGGTG AGTGGCATCA TAAGCCCCAG TGACCTTCCA 
GATAGTCCTG GGCAGACCAT GCCACCAAGC AGAAGCAAAA CCCCTCCACC TCCTCCACAG 
ACAGTGCAGG CCAAGAGAGA GGTGCCAAAA AGTAAAGTCC CTGCTGCTGA GAAGAGAGAG 
AGTGGGCCTA AGCAGACTGC TGTAAATGCT GCCGTGCAGA GGGTGCAGGT CCTTCCAGAC 
GTGGATACTT TGTTACACTT CGCCACAGAA AGTACTCCAG ACGGGTTTTC TTGTTCCTCC 
AGCCTAAGTG CTCTGAGCCT GGATGAGCCA TTTATACAGA AAGATGTAGA ATTAAGAATC 
ATGCCTCCAG TTCAGGAAAA CGACAATGGG AATGAAACTG AATCAGAACA GCCTGAGGAA 
TCAAATGAAA ACCAGGATAA AGAGGTAGAA AAGCCTGACT CTGAAAAAGA CTTATTAGAT 
GATTCTGATG ACGATGATAT TGAAATATTA GAAGAATGTA TTATTTCAGC CATGCCAACA 
AAGTCATCAC GCAAAGCCAA AAAACTAGCC CAGACTGCTT CAAAATTACC TCCACCTGTG 
GCAAGGAAAC CAAGTCAGCT ACCTGTGTAT AAACTTCTGC CAGCACAGAA TAGGCTGCAG 
GCACAAAAAC ATGTTAGCTT TACACCAGGG GATGATGTGC CCCGGGTGTA CTGTGTAGAA 
GGGACACCTA TAAACTTTTC CACAGCAACG TCTCTAAGTG ATCTGACAAT AGAGTCCCCT 
CCAAATGAAT TGGCTACTGG AGATGGGGTC AGAGCGGGTA TACAGTCAGG TGAATTTGAA 
AAACGAGATA CCATTCCTAC AGAAGGCAGA AGTACAGATG ATGCTCAGCG AGGAAAAATC 
TCATCTATAG TTACACCAGA CCTGGATGAC AACAAAGCAG AGGAAGGAGA TATTCTTGCA 
GAATGTATCA ATTCTGCTAT GCCCAAAGGA AAAAGCCACA AGCCTTTCCG AGTGAAAAAG 
ATAATGGACC AAGTCCAACA AGCATCCTCG ACTTCATCTG GAGCTAACAA AAATCAAGTA 
GACACTAAGA AAAAGAAGCC TACTTCACCA GTAAAGCCCA TGCCACAAAA TACTGAATAT 
AGAACGCGTG TGAGAAAGAA TACAGACTCA AAAGTTAATG TAAATACTGA AGAAACTTTC 
TCAGACAACA AAGACTCAAA GAAACCAAGC TTACAAACCA ATGCCAAGGC CTTCAATGAA 
AAGCTACCTA ACAATGAAGA CAGAGTGCGG GGGAGCTTCG CCTTGGACTC ACCGCATCAC 
TACACCCCTA TTGAGGGGAC GCCGTACTGC TTTTCCCGAA ATGACTCCTT GAGTTCTCTG 
GATTTTGATG ATGACGATGT TGACCTTTCC AGGGAAAAGG CCGAGTTAAG AAAGGGCAAA 
GAAAGCAAGG ATTCCGAAGC CAAAGTTACC TGCCGCCCAG AACCAAACTC AAGCCAGCAG 
GCAGCTAGTA AGTCACAAGC CAGTATAAAA CATCCAGCAA ACAGAGCACA GTCCAAACCA 
GTGCTGCAGA AACAGCCCAC TTTCCCCCAG TCCTCCAAAG ACGGACCAGA TAGAGGGGCA 
GCAACTGACG AAAAACTGCA GAATTTTGCT ATTGAAAATA CTCCAGTTTG CTTTTCTCGA 
AATTCCTCTC TGAGTTCCCT TAGTGACATT GACCAGGAAA ACAACAATAA CAAAGAAAGT 
GAACCAATCA AAGAAGCTGA ACCTGCCAAC TCACAAGGAG AGCCCAGTAA GCCTCAGGCA 
TCCGGGTATG CTCCCAAGTC CTTCCACGTC GAAGACACCC CTGTCTGTTT CTCAAGAAAC 
AGCTCTCTCA GTTCTCTTAG CATTGACTCT GAGGACGACC TGTTACAGGA GTGTATAAGT 
TCTGCCATGC CAAAAAAGAA AAGGCCTTCA AGACTCAAGA GTGAGAGCGA AAAGCAGAGC 
CCTAGAAAAG TGGGTGGCAT ATTAGCTGAA GACCTGACGC TTGATTTGAA AGATCTACAG 
AGGCCAGATT CAGAACACGC TTTCTCCCCC GACTCAGAAA ATTTTGACTG GAAAGCTATT 
CAGGAAGGCG CAAACTCCAT AGTAAGTAGT TTGCACCAAG CTGCTGCAGC CGCCGCGTGC 
TTATCTAGAC AAGCGTCATC CGACTCAGAT TCCATTCTGT CACTAAAGTC CGGCATTTCT 
CTGGGATCGC CTTTTCATCT TACACCTGAT CAAGAGGAAA AGCCATTCAC AAGCAATAAA 
GGCCCAAGAA TTCTCAAACC TGGAGAGAAA AGCACATTAG AAGCAAAAAA AATAGAATCT 
GAAAACAAAG GAATCAAAGG CGGGAAAAAG GTTTATAAAA GCTTGATTAC GGGAAAGATT 
CGCTCCAATT CAGAAATTTC CAGCCAAATG AAACAACCCC TCCCGACAAA CATGCCTTCA 
ATCTCAAGAG GCAGGACGAT GATTCACATC CCAGGGCTTC GGAATAGCTC CTCTAGTACA 
AGCCCTGTCT CTAAGAAAGG CCCACCCCTC AAGACTCCAG CCTCTAAAAG CCCCAGTGAA 
GGGCCGGGAG CTACCACTTC TCCTCGAGGA ACTAAGCCAG CAGGAAAGTC AGAGCTTAGC 
CCTATCACCA GGCAAACTTC CCAAATCAGT GGGTCAAATA AGGGGTCTTC TAGATCAGGA 
TCTAGAGACT CCACTCCCTC AAGACCTACA CAGCAACCAT TAAGTAGGCC AATGCAGTCT 
CCAGGGCGAA ACTCAATTTC CCCTGGTAGA AATGGAATAA GCCCTCCTAA CAAACTGTCT 
CAGCTGCCCA GAACATCATC TCCCAGTACT GCTTCAACTA AGTCCTCCGG TTCTGGGAAA 
ATGTCATATA CATCCCCAGG TAGACAGCTG AGCCAACAAA ATCTTACCAA ACAAGCAAGT 
TTATCCAAGA ATGCCAGCAG TATCCCCAGA AGTGAGTCGG CATCTAAAGG ACTGAATCAG 
ATGAGTAACG GCAATGGGTC AAATAAAAAG GTAGAACTTT CTAGAATGTC TTCAACTAAA 
TCAAGTGGAA GTGAATCAGA CAGATCAGAA AGGCCTGCAT TAGTACGCCA GTCTACTTTC 
ATCAAAGAAG CCCCAAGCCC AACCCTGAGG AGGAAACTGG AGGAATCTGC CTCATTTGAA 
TCCCTTTCTC CATCTTCTAG ACCAGATTCT CCCACCAGGT CGCAGGCACA GACCCCAGTT 
TTAAGCCCTT CCCTTCCTGA TATGTCTCTG TCCACACATC CATCTGTTCA GGCAGGTGGG 
TGGCGAAAGC TCCCGCCTAA TCTCAGCCCC ACTATCGAGT ATAATGACGG AAGGCCCACA 
AAACGGCATG ATATTGCACG CTCCCATTCT GAAAGTCCTT CCAGACTACC AATCAACCGG 
GCGGGAACCT GGAAGCGTGA ACACAGCAAA CATTCCTCGT CCCTTCCTCG AGTGAGTACT 
TGGAGAAGAA CTGGAAGCTC ATCTTCTATT CTTTCTGCTT CATCAGAGTC CAGTGAAAAA 
GCAAAAAGTG AGGATGAAAG GCATGTGAGC TCCATGCCAG CACCCAGACA GATGAAGGAA 
AACCAGGTGC CCACCAAAGG AACATGGAGG AAAATCAAGG AAAGTGACAT TTCTCCCACA 
GGCATGGCTT CTCAGAGCGC TTCCTCAGGT GCTGCCAGTG GTGCTGAATC CAAGCCTCTG 
ATCTATCAGA TGGCACCTCC TGTCTCTAAA ACAGAGGATG TTTGGGTGAG AATTGAGGAC 
TGCCCCATTA ACAACCCTAG ATCTGGACGG TCCCCCACAG GCAACACCCC CCCAGTGATT 
GACAGTGTTT CAGAGAAGGG AAGTTCAAGC ATTAAAGATT CAAAAGACAC CCATGGGAAA 
CAGAGTGTGG GCAGTGGCAG TCCTGTGCAA ACCGTGGGTC TGGAAACCCG CCTCAACTCC 
TTTGTTCAGG TAGAGGCCCC AGAACAGAAA GGAACTGAGG CAAAACCAGG ACAGAGTAAC 
CCAGTCTCTA TAGCAGAGAC TGCTGAGACG TGTATAGCAG AGCGTACCCC TTTCAGTTCC 
AGTAGCTCCA GCAAGCACAG CTCACCTAGC GGGACTGTTG CTGCCAGAGT GACACCTTTT 
AATTACAACC CTAGCCCTAG GAAGAGCAGC GCAGACAGCA CTTCAGCCCG GCCGTCTCAG 
ATCCCTACGC CAGTGAGCAC CAACACGAAG AAGAGAGATT CGAAGACTGA CAGCACAGAA 
TCCAGTGGAG CCCAAAGTCC TAAACGCCAT TCCGGGTCTT ACCTCGTGAC GTCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106150.1
CDS	413..8692
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250290.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250290.1

ORF Insert Sequence:

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGGTCAT CTCAGAGCAG GCATGATGCT GCCTCCCATG AAGCTGGCCG GCAGCACGAA 
GGCCACGGAG TGGCAGAAAG CAACACCGCA GCCTCCAGTA GTGGTCAGAG TCCAGCTACA 
CGTGTGGATC ACGAAACAGC CAGTGTTTTG AGTTCTAGCG GCACGCACTC TGCTCCTCGA 
AGGTTGACAA GTCATCTGGG GACAAAGATA CGAGCTTACT GTGAAACCTG TTGGGAGTGG 
CAGGAAGCCC ACGAACAAGG CATGGACCAG GACAAAAACC CAATGCCAGC TCCTGTTGAG 
CATCAGATCT GTCCTGCTGT GTGTGTTCTA ATGAAGCTTT CATTTGATGA AGAGCATAGG 
CATGCAATGA ATGAACTTGG TAGGAAGGCT ACCCGGGGCA TTTCATCCCA GGAGCTAGGT 
CAGGGGCTTT CAGGGGGACT GCAGGCCATT GCAGAGTTAT TGCAGGTGGA CTGTGAGATG 
TATGGGCTTA CTAATGACCA CTACAGTGTT ACTTTAAGAC GGTATGCTGG AATGGCTTTG 
ACAAACTTGA CCTTTGGAGA TGTTGCCAAC AAGGCTACGC TGTGTTCTAT GAAAGGCTGC 
ATGAGAGCAC TTGTGGCCCA GTTAAAATCT GAGAGTGAAG ACTTACAGCA GGTTATTGCA 
AGTGTTTTGA GGAATTTGTC TTGGCGAGCA GATGTAAATA GCAAAAAGAC GTTGAGAGAA 
GTTGGAAGTG TGAAAGCATT GATGGAATGT GCTTTGGAAG TTAAAAAGGA ATCAACCCTC 
AAAAGCGTTT TGAGTGCCTT ATGGAACCTG TCTGCACACT GCACTGAGAA TAAGGCTGAC 
ATCTGTGCTG TGGATGGAGC ACTGGCATTT CTGGTTGGCA CCCTCACTTA CCGGAGCCAG 
ACAAATACTT TAGCCATTAT TGAAAGTGGA GGTGGGATAT TACGGAATGT GTCCAGCTTG 
ATAGCTACAA ACGAAGACCA CAGGCAAATC CTAAGAGAGA ACAATTGCCT ACAAACTTTA 
TTACAGCACT TGAAATCTCA CAGCTTGACA ATAGTCAGTA ATGCATGTGG AACTTTGTGG 
AATCTCTCAG CAAGAAATCC TAAAGACCAG GAAGCCTTGT GGGACATGGG GGCAGTGAGC 
ATGCTCAAGA ACCTCATTCA TTCCAAGCAC AAAATGATTG CCATGGGAAG TGCAGCAGCT 
TTAAGGAATC TCATGGCAAA CAGACCTGCA AAGTATAAGG ATGCCAATAT CATGTCTCCC 
GGCTCAAGTC TGCCATCCCT TCACGTTAGG AAACAGAAAG CTCTAGAAGC TGAGCTAGAT 
GCTCAGCATT TATCAGAAAC CTTCGACAAC ATTGACAACC TAAGTCCCAA GGCCTCTCAC 
CGGAGTAAGC AGAGACACAA GCAGAATCTT TATGGTGACT ATGCTTTTGA CGCCAATCGA 
CATGATGATA GTAGGTCAGA CAATTTCAAT ACTGGAAACA TGACTGTTCT TTCACCATAT 
TTAAATACTA CGGTATTGCC CAGCTCTTCT TCCTCAAGGG GAAGTTTAGA CAGTTCTCGT 
TCTGAGAAAG ACAGAAGTTT GGAGAGAGAG CGAGGTATTG GCCTCAGTGC TTACCATCCA 
ACAACAGAAA ATGCAGGAAC CTCATCAAAA CGAGGTCTGC AGATCACTAC CACTGCAGCC 
CAGATAGCCA AAGTTATGGA AGAAGTATCA GCCATTCATA CCTCCCAGGA CGACAGAAGT 
TCTGCTTCTA CCACCGAGTT CCATTGTGTG GCAGACGACA GGAGTGCGGC ACGAAGAAGC 
TCTGCCTCCC ACACACACTC AAACACATAC AACTTCACTA AGTCGGAAAA TTCAAATAGG 
ACATGCTCTA TGCCTTATGC CAAAGTGGAA TATAAACGAT CTTCAAATGA CAGTTTAAAT 
AGTGTCACTA GTAGTGATGG ATATGGTAAA AGAGGCCAAA TGAAACCCTC AGTTGAATCC 
TATTCTGAAG ATGATGAAAG TAAATTTTGC AGTTATGGTC AGTATCCAGC TGACCTAGCC 
CATAAGATAC ACAGTGCAAA TCATATGGAT GATAATGATG GAGAACTGGA TACACCAATA 
AATTACAGTC TTAAATATTC AGATGAGCAG TTGAACTCAG GAAGGCAGAG TCCCTCACAG 
AATGAAAGGT GGGCAAGACC AAAGCATGTG ATAGAAGATG AAATAAAGCA AAACGAGCAA 
AGACAAGCAA GAAGCCAGAA CACCAGTTAT CCTGTCTATT CTGAGAATAC CGATGACAAA 
CACCTCAAAT TCCAACCACA TTTTGGACAA CAAGAATGTG TTTCCCCATA TAGGTCAAGG 
GGAACCAGTG GTTCAGAAAC AAATCGAATG GGTTCTAGTC ATGCAATTAA TCAAAATGTA 
AACCAGTCTC TGTGTCAGGA AGATGATTAT GAAGATGATA AACCTACCAA CTACAGTGAA 
CGTTATTCTG AGGAAGAACA ACATGAAGAA GAAGAAGAGA GACCGACAAA TTATAGCATA 
AAATATAATG AAGAGAAACA TCATGTGGAT CAGCCTATTG ATTATAGTTT AAAATATGCC 
ACTGACATTT CTTCCTCACA AAAACCATCA TTTTCATTCT CAAAGAATTC ATCAGCACAA 
AGCACTAAAC CTGAACATCT CTCTCCAAGC AGCGAGAATA CAGCTGTACC TCCATCTAAT 
GCCAAAAGGC AGAATCAGCT GCGTCCAAGT TCAGCACAAA GAAATGGCCA GACTCAAAAA 
GGCACTACTT GCAAAGTCCC CTCCATCAAC CAAGAAACAA TACAGACTTA CTGCGTAGAA 
GACACCCCAA TATGTTTTTC AAGGTGCAGT TCATTATCAT CACTGTCATC AGCTGACGAT 
GAAATAGGAT GTGATCAGAC AACACAGGAA GCAGATTCTG CTAATACTCT GCAGACAGCA 
GAAGTAAAAG AGAATGATGT AACTCGGTCA GCTGAAGATC CTGCAACTGA AGTTCCAGCA 
GTGTCCCAGA ATGCTAGAGC CAAACCCAGC CGACTCCAGG CTTCTGGCTT ATCTTCAGAA 
TCAACCAGGC ATAATAAAGC TGTTGAGTTT TCTTCAGGAG CCAAGTCTCC CTCCAAAAGT 
GGTGCTCAGA CACCCAAAAG TCCCCCAGAA CACTATGTCC AGGAGACTCC GCTCGTATTC 
AGCAGGTGTA CTTCTGTCAG CTCCCTTGAC AGTTTTGAGA GTCGCTCCAT TGCCAGCTCT 
GTTCAGAGTG AGCCATGTAG TGGAATGGTG AGTGGCATCA TAAGCCCCAG TGACCTTCCA 
GATAGTCCTG GGCAGACCAT GCCACCAAGC AGAAGCAAAA CCCCTCCACC TCCTCCACAG 
ACAGTGCAGG CCAAGAGAGA GGTGCCAAAA AGTAAAGTCC CTGCTGCTGA GAAGAGAGAG 
AGTGGGCCTA AGCAGACTGC TGTAAATGCT GCCGTGCAGA GGGTGCAGGT CCTTCCAGAC 
GTGGATACTT TGTTACACTT CGCCACAGAA AGTACTCCAG ACGGGTTTTC TTGTTCCTCC 
AGCCTAAGTG CTCTGAGCCT GGATGAGCCA TTTATACAGA AAGATGTAGA ATTAAGAATC 
ATGCCTCCAG TTCAGGAAAA CGACAATGGG AATGAAACTG AATCAGAACA GCCTGAGGAA 
TCAAATGAAA ACCAGGATAA AGAGGTAGAA AAGCCTGACT CTGAAAAAGA CTTATTAGAT 
GATTCTGATG ACGATGATAT TGAAATATTA GAAGAATGTA TTATTTCAGC CATGCCAACA 
AAGTCATCAC GCAAAGCCAA AAAACTAGCC CAGACTGCTT CAAAATTACC TCCACCTGTG 
GCAAGGAAAC CAAGTCAGCT ACCTGTGTAT AAACTTCTGC CAGCACAGAA TAGGCTGCAG 
GCACAAAAAC ATGTTAGCTT TACACCAGGG GATGATGTGC CCCGGGTGTA CTGTGTAGAA 
GGGACACCTA TAAACTTTTC CACAGCAACG TCTCTAAGTG ATCTGACAAT AGAGTCCCCT 
CCAAATGAAT TGGCTACTGG AGATGGGGTC AGAGCGGGTA TACAGTCAGG TGAATTTGAA 
AAACGAGATA CCATTCCTAC AGAAGGCAGA AGTACAGATG ATGCTCAGCG AGGAAAAATC 
TCATCTATAG TTACACCAGA CCTGGATGAC AACAAAGCAG AGGAAGGAGA TATTCTTGCA 
GAATGTATCA ATTCTGCTAT GCCCAAAGGA AAAAGCCACA AGCCTTTCCG AGTGAAAAAG 
ATAATGGACC AAGTCCAACA AGCATCCTCG ACTTCATCTG GAGCTAACAA AAATCAAGTA 
GACACTAAGA AAAAGAAGCC TACTTCACCA GTAAAGCCCA TGCCACAAAA TACTGAATAT 
AGAACGCGTG TGAGAAAGAA TACAGACTCA AAAGTTAATG TAAATACTGA AGAAACTTTC 
TCAGACAACA AAGACTCAAA GAAACCAAGC TTACAAACCA ATGCCAAGGC CTTCAATGAA 
AAGCTACCTA ACAATGAAGA CAGAGTGCGG GGGAGCTTCG CCTTGGACTC ACCGCATCAC 
TACACCCCTA TTGAGGGGAC GCCGTACTGC TTTTCCCGAA ATGACTCCTT GAGTTCTCTG 
GATTTTGATG ATGACGATGT TGACCTTTCC AGGGAAAAGG CCGAGTTAAG AAAGGGCAAA 
GAAAGCAAGG ATTCCGAAGC CAAAGTTACC TGCCGCCCAG AACCAAACTC AAGCCAGCAG 
GCAGCTAGTA AGTCACAAGC CAGTATAAAA CATCCAGCAA ACAGAGCACA GTCCAAACCA 
GTGCTGCAGA AACAGCCCAC TTTCCCCCAG TCCTCCAAAG ACGGACCAGA TAGAGGGGCA 
GCAACTGACG AAAAACTGCA GAATTTTGCT ATTGAAAATA CTCCAGTTTG CTTTTCTCGA 
AATTCCTCTC TGAGTTCCCT TAGTGACATT GACCAGGAAA ACAACAATAA CAAAGAAAGT 
GAACCAATCA AAGAAGCTGA ACCTGCCAAC TCACAAGGAG AGCCCAGTAA GCCTCAGGCA 
TCCGGGTATG CTCCCAAGTC CTTCCACGTC GAAGACACCC CTGTCTGTTT CTCAAGAAAC 
AGCTCTCTCA GTTCTCTTAG CATTGACTCT GAGGACGACC TGTTACAGGA GTGTATAAGT 
TCTGCCATGC CAAAAAAGAA AAGGCCTTCA AGACTCAAGA GTGAGAGCGA AAAGCAGAGC 
CCTAGAAAAG TGGGTGGCAT ATTAGCTGAA GACCTGACGC TTGATTTGAA AGATCTACAG 
AGGCCAGATT CAGAACACGC TTTCTCCCCC GACTCAGAAA ATTTTGACTG GAAAGCTATT 
CAGGAAGGCG CAAACTCCAT AGTAAGTAGT TTGCACCAAG CTGCTGCAGC CGCCGCGTGC 
TTATCTAGAC AAGCGTCATC CGACTCAGAT TCCATTCTGT CACTAAAGTC CGGCATTTCT 
CTGGGATCGC CTTTTCATCT TACACCTGAT CAAGAGGAAA AGCCATTCAC AAGCAATAAA 
GGCCCAAGAA TTCTCAAACC TGGAGAGAAA AGCACATTAG AAGCAAAAAA AATAGAATCT 
GAAAACAAAG GAATCAAAGG CGGGAAAAAG GTTTATAAAA GCTTGATTAC GGGAAAGATT 
CGCTCCAATT CAGAAATTTC CAGCCAAATG AAACAACCCC TCCCGACAAA CATGCCTTCA 
ATCTCAAGAG GCAGGACGAT GATTCACATC CCAGGGCTTC GGAATAGCTC CTCTAGTACA 
AGCCCTGTCT CTAAGAAAGG CCCACCCCTC AAGACTCCAG CCTCTAAAAG CCCCAGTGAA 
GGGCCGGGAG CTACCACTTC TCCTCGAGGA ACTAAGCCAG CAGGAAAGTC AGAGCTTAGC 
CCTATCACCA GGCAAACTTC CCAAATCAGT GGGTCAAATA AGGGGTCTTC TAGATCAGGA 
TCTAGAGACT CCACTCCCTC AAGACCTACA CAGCAACCAT TAAGTAGGCC AATGCAGTCT 
CCAGGGCGAA ACTCAATTTC CCCTGGTAGA AATGGAATAA GCCCTCCTAA CAAACTGTCT 
CAGCTGCCCA GAACATCATC TCCCAGTACT GCTTCAACTA AGTCCTCCGG TTCTGGGAAA 
ATGTCATATA CATCCCCAGG TAGACAGCTG AGCCAACAAA ATCTTACCAA ACAAGCAAGT 
TTATCCAAGA ATGCCAGCAG TATCCCCAGA AGTGAGTCGG CATCTAAAGG ACTGAATCAG 
ATGAGTAACG GCAATGGGTC AAATAAAAAG GTAGAACTTT CTAGAATGTC TTCAACTAAA 
TCAAGTGGAA GTGAATCAGA CAGATCAGAA AGGCCTGCAT TAGTACGCCA GTCTACTTTC 
ATCAAAGAAG CCCCAAGCCC AACCCTGAGG AGGAAACTGG AGGAATCTGC CTCATTTGAA 
TCCCTTTCTC CATCTTCTAG ACCAGATTCT CCCACCAGGT CGCAGGCACA GACCCCAGTT 
TTAAGCCCTT CCCTTCCTGA TATGTCTCTG TCCACACATC CATCTGTTCA GGCAGGTGGG 
TGGCGAAAGC TCCCGCCTAA TCTCAGCCCC ACTATCGAGT ATAATGACGG AAGGCCCACA 
AAACGGCATG ATATTGCACG CTCCCATTCT GAAAGTCCTT CCAGACTACC AATCAACCGG 
GCGGGAACCT GGAAGCGTGA ACACAGCAAA CATTCCTCGT CCCTTCCTCG AGTGAGTACT 
TGGAGAAGAA CTGGAAGCTC ATCTTCTATT CTTTCTGCTT CATCAGAGTC CAGTGAAAAA 
GCAAAAAGTG AGGATGAAAG GCATGTGAGC TCCATGCCAG CACCCAGACA GATGAAGGAA 
AACCAGGTGC CCACCAAAGG AACATGGAGG AAAATCAAGG AAAGTGACAT TTCTCCCACA 
GGCATGGCTT CTCAGAGCGC TTCCTCAGGT GCTGCCAGTG GTGCTGAATC CAAGCCTCTG 
ATCTATCAGA TGGCACCTCC TGTCTCTAAA ACAGAGGATG TTTGGGTGAG AATTGAGGAC 
TGCCCCATTA ACAACCCTAG ATCTGGACGG TCCCCCACAG GCAACACCCC CCCAGTGATT 
GACAGTGTTT CAGAGAAGGG AAGTTCAAGC ATTAAAGATT CAAAAGACAC CCATGGGAAA 
CAGAGTGTGG GCAGTGGCAG TCCTGTGCAA ACCGTGGGTC TGGAAACCCG CCTCAACTCC 
TTTGTTCAGG TAGAGGCCCC AGAACAGAAA GGAACTGAGG CAAAACCAGG ACAGAGTAAC 
CCAGTCTCTA TAGCAGAGAC TGCTGAGACG TGTATAGCAG AGCGTACCCC TTTCAGTTCC 
AGTAGCTCCA GCAAGCACAG CTCACCTAGC GGGACTGTTG CTGCCAGAGT GACACCTTTT 
AATTACAACC CTAGCCCTAG GAAGAGCAGC GCAGACAGCA CTTCAGCCCG GCCGTCTCAG 
ATCCCTACGC CAGTGAGCAC CAACACGAAG AAGAGAGATT CGAAGACTGA CAGCACAGAA 
TCCAGTGGAG CCCAAAGTCC TAAACGCCAT TCCGGGTCTT ACCTCGTGAC GTCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250291.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106151.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X22, mRNA.

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CloneID	OMu87329
Clone ID Related Accession (Same CDS sequence)	XM_030250291.1
Accession Version	XM_030250291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGATA 
CGAGCTTACT GTGAAACCTG TTGGGAGTGG CAGGAAGCCC ACGAACAAGG CATGGACCAG 
GACAAAAACC CAATGCCAGC TCCTGTTGAG CATCAGATCT GTCCTGCTGT GTGTGTTCTA 
ATGAAGCTTT CATTTGATGA AGAGCATAGG CATGCAATGA ATGAACTTGG GGGACTGCAG 
GCCATTGCAG AGTTATTGCA GGTGGACTGT GAGATGTATG GGCTTACTAA TGACCACTAC 
AGTGTTACTT TAAGACGGTA TGCTGGAATG GCTTTGACAA ACTTGACCTT TGGAGATGTT 
GCCAACAAGG CTACGCTGTG TTCTATGAAA GGCTGCATGA GAGCACTTGT GGCCCAGTTA 
AAATCTGAGA GTGAAGACTT ACAGCAGGTT ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG 
CGAGCAGATG TAAATAGCAA AAAGACGTTG AGAGAAGTTG GAAGTGTGAA AGCATTGATG 
GAATGTGCTT TGGAAGTTAA AAAGGAATCA ACCCTCAAAA GCGTTTTGAG TGCCTTATGG 
AACCTGTCTG CACACTGCAC TGAGAATAAG GCTGACATCT GTGCTGTGGA TGGAGCACTG 
GCATTTCTGG TTGGCACCCT CACTTACCGG AGCCAGACAA ATACTTTAGC CATTATTGAA 
AGTGGAGGTG GGATATTACG GAATGTGTCC AGCTTGATAG CTACAAACGA AGACCACAGG 
CAAATCCTAA GAGAGAACAA TTGCCTACAA ACTTTATTAC AGCACTTGAA ATCTCACAGC 
TTGACAATAG TCAGTAATGC ATGTGGAACT TTGTGGAATC TCTCAGCAAG AAATCCTAAA 
GACCAGGAAG CCTTGTGGGA CATGGGGGCA GTGAGCATGC TCAAGAACCT CATTCATTCC 
AAGCACAAAA TGATTGCCAT GGGAAGTGCA GCAGCTTTAA GGAATCTCAT GGCAAACAGA 
CCTGCAAAGT ATAAGGATGC CAATATCATG TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC 
GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG CTAGATGCTC AGCATTTATC AGAAACCTTC 
GACAACATTG ACAACCTAAG TCCCAAGGCC TCTCACCGGA GTAAGCAGAG ACACAAGCAG 
AATCTTTATG GTGACTATGC TTTTGACGCC AATCGACATG ATGATAGTAG GTCAGACAAT 
TTCAATACTG GAAACATGAC TGTTCTTTCA CCATATTTAA ATACTACGGT ATTGCCCAGC 
TCTTCTTCCT CAAGGGGAAG TTTAGACAGT TCTCGTTCTG AGAAAGACAG AAGTTTGGAG 
AGAGAGCGAG GTATTGGCCT CAGTGCTTAC CATCCAACAA CAGAAAATGC AGGAACCTCA 
TCAAAACGAG GTCTGCAGAT CACTACCACT GCAGCCCAGA TAGCCAAAGT TATGGAAGAA 
GTATCAGCCA TTCATACCTC CCAGGACGAC AGAAGTTCTG CTTCTACCAC CGAGTTCCAT 
TGTGTGGCAG ACGACAGGAG TGCGGCACGA AGAAGCTCTG CCTCCCACAC ACACTCAAAC 
ACATACAACT TCACTAAGTC GGAAAATTCA AATAGGACAT GCTCTATGCC TTATGCCAAA 
GTGGAATATA AACGATCTTC AAATGACAGT TTAAATAGTG TCACTAGTAG TGATGGATAT 
GGTAAAAGAG GCCAAATGAA ACCCTCAGTT GAATCCTATT CTGAAGATGA TGAAAGTAAA 
TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC CTAGCCCATA AGATACACAG TGCAAATCAT 
ATGGATGATA ATGATGGAGA ACTGGATACA CCAATAAATT ACAGTCTTAA ATATTCAGAT 
GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC TCACAGAATG AAAGGTGGGC AAGACCAAAG 
CATGTGATAG AAGATGAAAT AAAGCAAAAC GAGCAAAGAC AAGCAAGAAG CCAGAACACC 
AGTTATCCTG TCTATTCTGA GAATACCGAT GACAAACACC TCAAATTCCA ACCACATTTT 
GGACAACAAG AATGTGTTTC CCCATATAGG TCAAGGGGAA CCAGTGGTTC AGAAACAAAT 
CGAATGGGTT CTAGTCATGC AATTAATCAA AATGTAAACC AGTCTCTGTG TCAGGAAGAT 
GATTATGAAG ATGATAAACC TACCAACTAC AGTGAACGTT ATTCTGAGGA AGAACAACAT 
GAAGAAGAAG AAGAGAGACC GACAAATTAT AGCATAAAAT ATAATGAAGA GAAACATCAT 
GTGGATCAGC CTATTGATTA TAGTTTAAAA TATGCCACTG ACATTTCTTC CTCACAAAAA 
CCATCATTTT CATTCTCAAA GAATTCATCA GCACAAAGCA CTAAACCTGA ACATCTCTCT 
CCAAGCAGCG AGAATACAGC TGTACCTCCA TCTAATGCCA AAAGGCAGAA TCAGCTGCGT 
CCAAGTTCAG CACAAAGAAA TGGCCAGACT CAAAAAGGCA CTACTTGCAA AGTCCCCTCC 
ATCAACCAAG AAACAATACA GACTTACTGC GTAGAAGACA CCCCAATATG TTTTTCAAGG 
TGCAGTTCAT TATCATCACT GTCATCAGCT GACGATGAAA TAGGATGTGA TCAGACAACA 
CAGGAAGCAG ATTCTGCTAA TACTCTGCAG ACAGCAGAAG TAAAAGAGAA TGATGTAACT 
CGGTCAGCTG AAGATCCTGC AACTGAAGTT CCAGCAGTGT CCCAGAATGC TAGAGCCAAA 
CCCAGCCGAC TCCAGGCTTC TGGCTTATCT TCAGAATCAA CCAGGCATAA TAAAGCTGTT 
GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC AAAAGTGGTG CTCAGACACC CAAAAGTCCC 
CCAGAACACT ATGTCCAGGA GACTCCGCTC GTATTCAGCA GGTGTACTTC TGTCAGCTCC 
CTTGACAGTT TTGAGAGTCG CTCCATTGCC AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA 
ATGGTGAGTG GCATCATAAG CCCCAGTGAC CTTCCAGATA GTCCTGGGCA GACCATGCCA 
CCAAGCAGAA GCAAAACCCC TCCACCTCCT CCACAGACAG TGCAGGCCAA GAGAGAGGTG 
CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG AGAGAGAGTG GGCCTAAGCA GACTGCTGTA 
AATGCTGCCG TGCAGAGGGT GCAGGTCCTT CCAGACGTGG ATACTTTGTT ACACTTCGCC 
ACAGAAAGTA CTCCAGACGG GTTTTCTTGT TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT 
GAGCCATTTA TACAGAAAGA TGTAGAATTA AGAATCATGC CTCCAGTTCA GGAAAACGAC 
AATGGGAATG AAACTGAATC AGAACAGCCT GAGGAATCAA ATGAAAACCA GGATAAAGAG 
GTAGAAAAGC CTGACTCTGA AAAAGACTTA TTAGATGATT CTGATGACGA TGATATTGAA 
ATATTAGAAG AATGTATTAT TTCAGCCATG CCAACAAAGT CATCACGCAA AGCCAAAAAA 
CTAGCCCAGA CTGCTTCAAA ATTACCTCCA CCTGTGGCAA GGAAACCAAG TCAGCTACCT 
GTGTATAAAC TTCTGCCAGC ACAGAATAGG CTGCAGGCAC AAAAACATGT TAGCTTTACA 
CCAGGGGATG ATGTGCCCCG GGTGTACTGT GTAGAAGGGA CACCTATAAA CTTTTCCACA 
GCAACGTCTC TAAGTGATCT GACAATAGAG TCCCCTCCAA ATGAATTGGC TACTGGAGAT 
GGGGTCAGAG CGGGTATACA GTCAGGTGAA TTTGAAAAAC GAGATACCAT TCCTACAGAA 
GGCAGAAGTA CAGATGATGC TCAGCGAGGA AAAATCTCAT CTATAGTTAC ACCAGACCTG 
GATGACAACA AAGCAGAGGA AGGAGATATT CTTGCAGAAT GTATCAATTC TGCTATGCCC 
AAAGGAAAAA GCCACAAGCC TTTCCGAGTG AAAAAGATAA TGGACCAAGT CCAACAAGCA 
TCCTCGACTT CATCTGGAGC TAACAAAAAT CAAGTAGACA CTAAGAAAAA GAAGCCTACT 
TCACCAGTAA AGCCCATGCC ACAAAATACT GAATATAGAA CGCGTGTGAG AAAGAATACA 
GACTCAAAAG TTAATGTAAA TACTGAAGAA ACTTTCTCAG ACAACAAAGA CTCAAAGAAA 
CCAAGCTTAC AAACCAATGC CAAGGCCTTC AATGAAAAGC TACCTAACAA TGAAGACAGA 
GTGCGGGGGA GCTTCGCCTT GGACTCACCG CATCACTACA CCCCTATTGA GGGGACGCCG 
TACTGCTTTT CCCGAAATGA CTCCTTGAGT TCTCTGGATT TTGATGATGA CGATGTTGAC 
CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG GGCAAAGAAA GCAAGGATTC CGAAGCCAAA 
GTTACCTGCC GCCCAGAACC AAACTCAAGC CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT 
ATAAAACATC CAGCAAACAG AGCACAGTCC AAACCAGTGC TGCAGAAACA GCCCACTTTC 
CCCCAGTCCT CCAAAGACGG ACCAGATAGA GGGGCAGCAA CTGACGAAAA ACTGCAGAAT 
TTTGCTATTG AAAATACTCC AGTTTGCTTT TCTCGAAATT CCTCTCTGAG TTCCCTTAGT 
GACATTGACC AGGAAAACAA CAATAACAAA GAAAGTGAAC CAATCAAAGA AGCTGAACCT 
GCCAACTCAC AAGGAGAGCC CAGTAAGCCT CAGGCATCCG GGTATGCTCC CAAGTCCTTC 
CACGTCGAAG ACACCCCTGT CTGTTTCTCA AGAAACAGCT CTCTCAGTTC TCTTAGCATT 
GACTCTGAGG ACGACCTGTT ACAGGAGTGT ATAAGTTCTG CCATGCCAAA AAAGAAAAGG 
CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG CAGAGCCCTA GAAAAGTGGG TGGCATATTA 
GCTGAAGACC TGACGCTTGA TTTGAAAGAT CTACAGAGGC CAGATTCAGA ACACGCTTTC 
TCCCCCGACT CAGAAAATTT TGACTGGAAA GCTATTCAGG AAGGCGCAAA CTCCATAGTA 
AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC GCGTGCTTAT CTAGACAAGC GTCATCCGAC 
TCAGATTCCA TTCTGTCACT AAAGTCCGGC ATTTCTCTGG GATCGCCTTT TCATCTTACA 
CCTGATCAAG AGGAAAAGCC ATTCACAAGC AATAAAGGCC CAAGAATTCT CAAACCTGGA 
GAGAAAAGCA CATTAGAAGC AAAAAAAATA GAATCTGAAA ACAAAGGAAT CAAAGGCGGG 
AAAAAGGTTT ATAAAAGCTT GATTACGGGA AAGATTCGCT CCAATTCAGA AATTTCCAGC 
CAAATGAAAC AACCCCTCCC GACAAACATG CCTTCAATCT CAAGAGGCAG GACGATGATT 
CACATCCCAG GGCTTCGGAA TAGCTCCTCT AGTACAAGCC CTGTCTCTAA GAAAGGCCCA 
CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC AGTGAAGGGC CGGGAGCTAC CACTTCTCCT 
CGAGGAACTA AGCCAGCAGG AAAGTCAGAG CTTAGCCCTA TCACCAGGCA AACTTCCCAA 
ATCAGTGGGT CAAATAAGGG GTCTTCTAGA TCAGGATCTA GAGACTCCAC TCCCTCAAGA 
CCTACACAGC AACCATTAAG TAGGCCAATG CAGTCTCCAG GGCGAAACTC AATTTCCCCT 
GGTAGAAATG GAATAAGCCC TCCTAACAAA CTGTCTCAGC TGCCCAGAAC ATCATCTCCC 
AGTACTGCTT CAACTAAGTC CTCCGGTTCT GGGAAAATGT CATATACATC CCCAGGTAGA 
CAGCTGAGCC AACAAAATCT TACCAAACAA GCAAGTTTAT CCAAGAATGC CAGCAGTATC 
CCCAGAAGTG AGTCGGCATC TAAAGGACTG AATCAGATGA GTAACGGCAA TGGGTCAAAT 
AAAAAGGTAG AACTTTCTAG AATGTCTTCA ACTAAATCAA GTGGAAGTGA ATCAGACAGA 
TCAGAAAGGC CTGCATTAGT ACGCCAGTCT ACTTTCATCA AAGAAGCCCC AAGCCCAACC 
CTGAGGAGGA AACTGGAGGA ATCTGCCTCA TTTGAATCCC TTTCTCCATC TTCTAGACCA 
GATTCTCCCA CCAGGTCGCA GGCACAGACC CCAGTTTTAA GCCCTTCCCT TCCTGATATG 
TCTCTGTCCA CACATCCATC TGTTCAGGCA GGTGGGTGGC GAAAGCTCCC GCCTAATCTC 
AGCCCCACTA TCGAGTATAA TGACGGAAGG CCCACAAAAC GGCATGATAT TGCACGCTCC 
CATTCTGAAA GTCCTTCCAG ACTACCAATC AACCGGGCGG GAACCTGGAA GCGTGAACAC 
AGCAAACATT CCTCGTCCCT TCCTCGAGTG AGTACTTGGA GAAGAACTGG AAGCTCATCT 
TCTATTCTTT CTGCTTCATC AGAGTCCAGT GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT 
GTGAGCTCCA TGCCAGCACC CAGACAGATG AAGGAAAACC AGGTGCCCAC CAAAGGAACA 
TGGAGGAAAA TCAAGGAAAG TGACATTTCT CCCACAGGCA TGGCTTCTCA GAGCGCTTCC 
TCAGGTGCTG CCAGTGGTGC TGAATCCAAG CCTCTGATCT ATCAGATGGC ACCTCCTGTC 
TCTAAAACAG AGGATGTTTG GGTGAGAATT GAGGACTGCC CCATTAACAA CCCTAGATCT 
GGACGGTCCC CCACAGGCAA CACCCCCCCA GTGATTGACA GTGTTTCAGA GAAGGGAAGT 
TCAAGCATTA AAGATTCAAA AGACACCCAT GGGAAACAGA GTGTGGGCAG TGGCAGTCCT 
GTGCAAACCG TGGGTCTGGA AACCCGCCTC AACTCCTTTG TTCAGGTAGA GGCCCCAGAA 
CAGAAAGGAA CTGAGGCAAA ACCAGGACAG AGTAACCCAG TCTCTATAGC AGAGACTGCT 
GAGACGTGTA TAGCAGAGCG TACCCCTTTC AGTTCCAGTA GCTCCAGCAA GCACAGCTCA 
CCTAGCGGGA CTGTTGCTGC CAGAGTGACA CCTTTTAATT ACAACCCTAG CCCTAGGAAG 
AGCAGCGCAG ACAGCACTTC AGCCCGGCCG TCTCAGATCC CTACGCCAGT GAGCACCAAC 
ACGAAGAAGA GAGATTCGAA GACTGACAGC ACAGAATCCA GTGGAGCCCA AAGTCCTAAA 
CGCCATTCCG GGTCTTACCT CGTGACGTCT GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106151.1
CDS	1..8256
Translation	MYAFLRSGPVAALPASVPPVTLGARSSGGGRGRVPGEGRRPGGARACGRGASVWQEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250291.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250291.1

ORF Insert Sequence:

ATGTACGCCT TCCTGCGCTC GGGGCCGGTC GCCGCGTTGC CCGCCTCCGT ACCGCCCGTG 
ACTCTCGGGG CCCGGAGCTC CGGCGGCGGC CGGGGTCGAG TCCCGGGGGA GGGGAGGCGC 
CCGGGCGGCG CCCGAGCTTG CGGCCGCGGA GCGAGCGTCT GGCAGGAAGT ACTTAAGCAG 
CTACAGGGAA GTATTGAAGA TGAGACTATG ACTTCTGGAC AGATTGACTT ACTAGAGCGT 
CTTAAAGAAT TTAACTTAGA TAGTAATTTC CCCGGAGTGA AACTACGCTC AAAAATGTCC 
CTTCGCTCCT ACGGAAGTCG GGAAGGATCT GTATCCAGCC GTTCAGGAGA ATGCAGTCCT 
GTCCCCATGG GGTCATTCCC AAGAAGAACA TTTGTAAATG GAAGCAGAGA GAGTACTGGG 
TATCTAGAAG AGCTTGAAAA AGAAAGATCA TTACTCCTTG CTGATCTTGA CAAAGAAGAG 
AAGGAAAAGG ACTGGTATTA TGCTCAACTT CAGAACCTCA CAAAAAGAAT AGATAGCCTG 
CCTTTAACTG AAAATTTTTC CTTACAGACA GACATGACAA GACGGCAGCT GGAGTATGAA 
GCAAGGCAGA TCAGGGCTGC AATGGAGGAG CAGCTTGGCA CCTGCCAGGA CATGGAGAAG 
CGTGCACAGC GAAGAATAGC CAGGATCCAG CAAATAGAAA AGGACATACT GCGCGTGCGC 
CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG GAGAGGTCAT CTCAGAGCAG GCATGATGCT 
GCCTCCCATG AAGCTGGCCG GCAGCACGAA GGCCACGGAG TGGCAGAAAG CAACACCGCA 
GCCTCCAGTA GTGGTCAGAG TCCAGCTACA CGTGTGGATC ACGAAACAGC CAGTGTTTTG 
AGTTCTAGCG GCACGCACTC TGCTCCTCGA AGGTTGACAA GTCATCTGGG GACAAAGATA 
CGAGCTTACT GTGAAACCTG TTGGGAGTGG CAGGAAGCCC ACGAACAAGG CATGGACCAG 
GACAAAAACC CAATGCCAGC TCCTGTTGAG CATCAGATCT GTCCTGCTGT GTGTGTTCTA 
ATGAAGCTTT CATTTGATGA AGAGCATAGG CATGCAATGA ATGAACTTGG GGGACTGCAG 
GCCATTGCAG AGTTATTGCA GGTGGACTGT GAGATGTATG GGCTTACTAA TGACCACTAC 
AGTGTTACTT TAAGACGGTA TGCTGGAATG GCTTTGACAA ACTTGACCTT TGGAGATGTT 
GCCAACAAGG CTACGCTGTG TTCTATGAAA GGCTGCATGA GAGCACTTGT GGCCCAGTTA 
AAATCTGAGA GTGAAGACTT ACAGCAGGTT ATTGCAAGTG TTTTGAGGAA TTTGTCTTGG 
CGAGCAGATG TAAATAGCAA AAAGACGTTG AGAGAAGTTG GAAGTGTGAA AGCATTGATG 
GAATGTGCTT TGGAAGTTAA AAAGGAATCA ACCCTCAAAA GCGTTTTGAG TGCCTTATGG 
AACCTGTCTG CACACTGCAC TGAGAATAAG GCTGACATCT GTGCTGTGGA TGGAGCACTG 
GCATTTCTGG TTGGCACCCT CACTTACCGG AGCCAGACAA ATACTTTAGC CATTATTGAA 
AGTGGAGGTG GGATATTACG GAATGTGTCC AGCTTGATAG CTACAAACGA AGACCACAGG 
CAAATCCTAA GAGAGAACAA TTGCCTACAA ACTTTATTAC AGCACTTGAA ATCTCACAGC 
TTGACAATAG TCAGTAATGC ATGTGGAACT TTGTGGAATC TCTCAGCAAG AAATCCTAAA 
GACCAGGAAG CCTTGTGGGA CATGGGGGCA GTGAGCATGC TCAAGAACCT CATTCATTCC 
AAGCACAAAA TGATTGCCAT GGGAAGTGCA GCAGCTTTAA GGAATCTCAT GGCAAACAGA 
CCTGCAAAGT ATAAGGATGC CAATATCATG TCTCCCGGCT CAAGTCTGCC ATCCCTTCAC 
GTTAGGAAAC AGAAAGCTCT AGAAGCTGAG CTAGATGCTC AGCATTTATC AGAAACCTTC 
GACAACATTG ACAACCTAAG TCCCAAGGCC TCTCACCGGA GTAAGCAGAG ACACAAGCAG 
AATCTTTATG GTGACTATGC TTTTGACGCC AATCGACATG ATGATAGTAG GTCAGACAAT 
TTCAATACTG GAAACATGAC TGTTCTTTCA CCATATTTAA ATACTACGGT ATTGCCCAGC 
TCTTCTTCCT CAAGGGGAAG TTTAGACAGT TCTCGTTCTG AGAAAGACAG AAGTTTGGAG 
AGAGAGCGAG GTATTGGCCT CAGTGCTTAC CATCCAACAA CAGAAAATGC AGGAACCTCA 
TCAAAACGAG GTCTGCAGAT CACTACCACT GCAGCCCAGA TAGCCAAAGT TATGGAAGAA 
GTATCAGCCA TTCATACCTC CCAGGACGAC AGAAGTTCTG CTTCTACCAC CGAGTTCCAT 
TGTGTGGCAG ACGACAGGAG TGCGGCACGA AGAAGCTCTG CCTCCCACAC ACACTCAAAC 
ACATACAACT TCACTAAGTC GGAAAATTCA AATAGGACAT GCTCTATGCC TTATGCCAAA 
GTGGAATATA AACGATCTTC AAATGACAGT TTAAATAGTG TCACTAGTAG TGATGGATAT 
GGTAAAAGAG GCCAAATGAA ACCCTCAGTT GAATCCTATT CTGAAGATGA TGAAAGTAAA 
TTTTGCAGTT ATGGTCAGTA TCCAGCTGAC CTAGCCCATA AGATACACAG TGCAAATCAT 
ATGGATGATA ATGATGGAGA ACTGGATACA CCAATAAATT ACAGTCTTAA ATATTCAGAT 
GAGCAGTTGA ACTCAGGAAG GCAGAGTCCC TCACAGAATG AAAGGTGGGC AAGACCAAAG 
CATGTGATAG AAGATGAAAT AAAGCAAAAC GAGCAAAGAC AAGCAAGAAG CCAGAACACC 
AGTTATCCTG TCTATTCTGA GAATACCGAT GACAAACACC TCAAATTCCA ACCACATTTT 
GGACAACAAG AATGTGTTTC CCCATATAGG TCAAGGGGAA CCAGTGGTTC AGAAACAAAT 
CGAATGGGTT CTAGTCATGC AATTAATCAA AATGTAAACC AGTCTCTGTG TCAGGAAGAT 
GATTATGAAG ATGATAAACC TACCAACTAC AGTGAACGTT ATTCTGAGGA AGAACAACAT 
GAAGAAGAAG AAGAGAGACC GACAAATTAT AGCATAAAAT ATAATGAAGA GAAACATCAT 
GTGGATCAGC CTATTGATTA TAGTTTAAAA TATGCCACTG ACATTTCTTC CTCACAAAAA 
CCATCATTTT CATTCTCAAA GAATTCATCA GCACAAAGCA CTAAACCTGA ACATCTCTCT 
CCAAGCAGCG AGAATACAGC TGTACCTCCA TCTAATGCCA AAAGGCAGAA TCAGCTGCGT 
CCAAGTTCAG CACAAAGAAA TGGCCAGACT CAAAAAGGCA CTACTTGCAA AGTCCCCTCC 
ATCAACCAAG AAACAATACA GACTTACTGC GTAGAAGACA CCCCAATATG TTTTTCAAGG 
TGCAGTTCAT TATCATCACT GTCATCAGCT GACGATGAAA TAGGATGTGA TCAGACAACA 
CAGGAAGCAG ATTCTGCTAA TACTCTGCAG ACAGCAGAAG TAAAAGAGAA TGATGTAACT 
CGGTCAGCTG AAGATCCTGC AACTGAAGTT CCAGCAGTGT CCCAGAATGC TAGAGCCAAA 
CCCAGCCGAC TCCAGGCTTC TGGCTTATCT TCAGAATCAA CCAGGCATAA TAAAGCTGTT 
GAGTTTTCTT CAGGAGCCAA GTCTCCCTCC AAAAGTGGTG CTCAGACACC CAAAAGTCCC 
CCAGAACACT ATGTCCAGGA GACTCCGCTC GTATTCAGCA GGTGTACTTC TGTCAGCTCC 
CTTGACAGTT TTGAGAGTCG CTCCATTGCC AGCTCTGTTC AGAGTGAGCC ATGTAGTGGA 
ATGGTGAGTG GCATCATAAG CCCCAGTGAC CTTCCAGATA GTCCTGGGCA GACCATGCCA 
CCAAGCAGAA GCAAAACCCC TCCACCTCCT CCACAGACAG TGCAGGCCAA GAGAGAGGTG 
CCAAAAAGTA AAGTCCCTGC TGCTGAGAAG AGAGAGAGTG GGCCTAAGCA GACTGCTGTA 
AATGCTGCCG TGCAGAGGGT GCAGGTCCTT CCAGACGTGG ATACTTTGTT ACACTTCGCC 
ACAGAAAGTA CTCCAGACGG GTTTTCTTGT TCCTCCAGCC TAAGTGCTCT GAGCCTGGAT 
GAGCCATTTA TACAGAAAGA TGTAGAATTA AGAATCATGC CTCCAGTTCA GGAAAACGAC 
AATGGGAATG AAACTGAATC AGAACAGCCT GAGGAATCAA ATGAAAACCA GGATAAAGAG 
GTAGAAAAGC CTGACTCTGA AAAAGACTTA TTAGATGATT CTGATGACGA TGATATTGAA 
ATATTAGAAG AATGTATTAT TTCAGCCATG CCAACAAAGT CATCACGCAA AGCCAAAAAA 
CTAGCCCAGA CTGCTTCAAA ATTACCTCCA CCTGTGGCAA GGAAACCAAG TCAGCTACCT 
GTGTATAAAC TTCTGCCAGC ACAGAATAGG CTGCAGGCAC AAAAACATGT TAGCTTTACA 
CCAGGGGATG ATGTGCCCCG GGTGTACTGT GTAGAAGGGA CACCTATAAA CTTTTCCACA 
GCAACGTCTC TAAGTGATCT GACAATAGAG TCCCCTCCAA ATGAATTGGC TACTGGAGAT 
GGGGTCAGAG CGGGTATACA GTCAGGTGAA TTTGAAAAAC GAGATACCAT TCCTACAGAA 
GGCAGAAGTA CAGATGATGC TCAGCGAGGA AAAATCTCAT CTATAGTTAC ACCAGACCTG 
GATGACAACA AAGCAGAGGA AGGAGATATT CTTGCAGAAT GTATCAATTC TGCTATGCCC 
AAAGGAAAAA GCCACAAGCC TTTCCGAGTG AAAAAGATAA TGGACCAAGT CCAACAAGCA 
TCCTCGACTT CATCTGGAGC TAACAAAAAT CAAGTAGACA CTAAGAAAAA GAAGCCTACT 
TCACCAGTAA AGCCCATGCC ACAAAATACT GAATATAGAA CGCGTGTGAG AAAGAATACA 
GACTCAAAAG TTAATGTAAA TACTGAAGAA ACTTTCTCAG ACAACAAAGA CTCAAAGAAA 
CCAAGCTTAC AAACCAATGC CAAGGCCTTC AATGAAAAGC TACCTAACAA TGAAGACAGA 
GTGCGGGGGA GCTTCGCCTT GGACTCACCG CATCACTACA CCCCTATTGA GGGGACGCCG 
TACTGCTTTT CCCGAAATGA CTCCTTGAGT TCTCTGGATT TTGATGATGA CGATGTTGAC 
CTTTCCAGGG AAAAGGCCGA GTTAAGAAAG GGCAAAGAAA GCAAGGATTC CGAAGCCAAA 
GTTACCTGCC GCCCAGAACC AAACTCAAGC CAGCAGGCAG CTAGTAAGTC ACAAGCCAGT 
ATAAAACATC CAGCAAACAG AGCACAGTCC AAACCAGTGC TGCAGAAACA GCCCACTTTC 
CCCCAGTCCT CCAAAGACGG ACCAGATAGA GGGGCAGCAA CTGACGAAAA ACTGCAGAAT 
TTTGCTATTG AAAATACTCC AGTTTGCTTT TCTCGAAATT CCTCTCTGAG TTCCCTTAGT 
GACATTGACC AGGAAAACAA CAATAACAAA GAAAGTGAAC CAATCAAAGA AGCTGAACCT 
GCCAACTCAC AAGGAGAGCC CAGTAAGCCT CAGGCATCCG GGTATGCTCC CAAGTCCTTC 
CACGTCGAAG ACACCCCTGT CTGTTTCTCA AGAAACAGCT CTCTCAGTTC TCTTAGCATT 
GACTCTGAGG ACGACCTGTT ACAGGAGTGT ATAAGTTCTG CCATGCCAAA AAAGAAAAGG 
CCTTCAAGAC TCAAGAGTGA GAGCGAAAAG CAGAGCCCTA GAAAAGTGGG TGGCATATTA 
GCTGAAGACC TGACGCTTGA TTTGAAAGAT CTACAGAGGC CAGATTCAGA ACACGCTTTC 
TCCCCCGACT CAGAAAATTT TGACTGGAAA GCTATTCAGG AAGGCGCAAA CTCCATAGTA 
AGTAGTTTGC ACCAAGCTGC TGCAGCCGCC GCGTGCTTAT CTAGACAAGC GTCATCCGAC 
TCAGATTCCA TTCTGTCACT AAAGTCCGGC ATTTCTCTGG GATCGCCTTT TCATCTTACA 
CCTGATCAAG AGGAAAAGCC ATTCACAAGC AATAAAGGCC CAAGAATTCT CAAACCTGGA 
GAGAAAAGCA CATTAGAAGC AAAAAAAATA GAATCTGAAA ACAAAGGAAT CAAAGGCGGG 
AAAAAGGTTT ATAAAAGCTT GATTACGGGA AAGATTCGCT CCAATTCAGA AATTTCCAGC 
CAAATGAAAC AACCCCTCCC GACAAACATG CCTTCAATCT CAAGAGGCAG GACGATGATT 
CACATCCCAG GGCTTCGGAA TAGCTCCTCT AGTACAAGCC CTGTCTCTAA GAAAGGCCCA 
CCCCTCAAGA CTCCAGCCTC TAAAAGCCCC AGTGAAGGGC CGGGAGCTAC CACTTCTCCT 
CGAGGAACTA AGCCAGCAGG AAAGTCAGAG CTTAGCCCTA TCACCAGGCA AACTTCCCAA 
ATCAGTGGGT CAAATAAGGG GTCTTCTAGA TCAGGATCTA GAGACTCCAC TCCCTCAAGA 
CCTACACAGC AACCATTAAG TAGGCCAATG CAGTCTCCAG GGCGAAACTC AATTTCCCCT 
GGTAGAAATG GAATAAGCCC TCCTAACAAA CTGTCTCAGC TGCCCAGAAC ATCATCTCCC 
AGTACTGCTT CAACTAAGTC CTCCGGTTCT GGGAAAATGT CATATACATC CCCAGGTAGA 
CAGCTGAGCC AACAAAATCT TACCAAACAA GCAAGTTTAT CCAAGAATGC CAGCAGTATC 
CCCAGAAGTG AGTCGGCATC TAAAGGACTG AATCAGATGA GTAACGGCAA TGGGTCAAAT 
AAAAAGGTAG AACTTTCTAG AATGTCTTCA ACTAAATCAA GTGGAAGTGA ATCAGACAGA 
TCAGAAAGGC CTGCATTAGT ACGCCAGTCT ACTTTCATCA AAGAAGCCCC AAGCCCAACC 
CTGAGGAGGA AACTGGAGGA ATCTGCCTCA TTTGAATCCC TTTCTCCATC TTCTAGACCA 
GATTCTCCCA CCAGGTCGCA GGCACAGACC CCAGTTTTAA GCCCTTCCCT TCCTGATATG 
TCTCTGTCCA CACATCCATC TGTTCAGGCA GGTGGGTGGC GAAAGCTCCC GCCTAATCTC 
AGCCCCACTA TCGAGTATAA TGACGGAAGG CCCACAAAAC GGCATGATAT TGCACGCTCC 
CATTCTGAAA GTCCTTCCAG ACTACCAATC AACCGGGCGG GAACCTGGAA GCGTGAACAC 
AGCAAACATT CCTCGTCCCT TCCTCGAGTG AGTACTTGGA GAAGAACTGG AAGCTCATCT 
TCTATTCTTT CTGCTTCATC AGAGTCCAGT GAAAAAGCAA AAAGTGAGGA TGAAAGGCAT 
GTGAGCTCCA TGCCAGCACC CAGACAGATG AAGGAAAACC AGGTGCCCAC CAAAGGAACA 
TGGAGGAAAA TCAAGGAAAG TGACATTTCT CCCACAGGCA TGGCTTCTCA GAGCGCTTCC 
TCAGGTGCTG CCAGTGGTGC TGAATCCAAG CCTCTGATCT ATCAGATGGC ACCTCCTGTC 
TCTAAAACAG AGGATGTTTG GGTGAGAATT GAGGACTGCC CCATTAACAA CCCTAGATCT 
GGACGGTCCC CCACAGGCAA CACCCCCCCA GTGATTGACA GTGTTTCAGA GAAGGGAAGT 
TCAAGCATTA AAGATTCAAA AGACACCCAT GGGAAACAGA GTGTGGGCAG TGGCAGTCCT 
GTGCAAACCG TGGGTCTGGA AACCCGCCTC AACTCCTTTG TTCAGGTAGA GGCCCCAGAA 
CAGAAAGGAA CTGAGGCAAA ACCAGGACAG AGTAACCCAG TCTCTATAGC AGAGACTGCT 
GAGACGTGTA TAGCAGAGCG TACCCCTTTC AGTTCCAGTA GCTCCAGCAA GCACAGCTCA 
CCTAGCGGGA CTGTTGCTGC CAGAGTGACA CCTTTTAATT ACAACCCTAG CCCTAGGAAG 
AGCAGCGCAG ACAGCACTTC AGCCCGGCCG TCTCAGATCC CTACGCCAGT GAGCACCAAC 
ACGAAGAAGA GAGATTCGAA GACTGACAGC ACAGAATCCA GTGGAGCCCA AAGTCCTAAA 
CGCCATTCCG GGTCTTACCT CGTGACGTCT GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250292.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106152.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X23, mRNA.

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CloneID	OMu87330
Clone ID Related Accession (Same CDS sequence)	XM_030250292.1
Accession Version	XM_030250292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGTC CAGCTACACG TGTGGATCAC GAAACAGCCA GTGTTTTGAG TTCTAGCGGC 
ACGCACTCTG CTCCTCGAAG GTTGACAAGT CATCTGGGGA CAAAGGTGGA AATGGTGTAT 
TCCTTGTTGT CAATGCTTGG TACTCATGAT AAGGACGATA TGTCACGAAC TTTGCTAGCT 
ATGTCCAGCT CCCAAGACAG CTGTATATCC ATGCGGCAGT CTGGATGTCT TCCTCTCCTC 
ATCCAGCTTT TACATGGCAA TGACAAAGAC TCTGTATTGT TGGGAAATTC CCGGGGCAGT 
AAAGAGGCTC GGGCCAGGGC CAGTGCAGCA CTCCACAACA TCATTCACTC ACAGCCTGAT 
GACAAGAGAG GCAGGCGTGA AATCCGAGTC CTTCATCTTT TGGAACAGAT ACGAGCTTAC 
TGTGAAACCT GTTGGGAGTG GCAGGAAGCC CACGAACAAG GCATGGACCA GGACAAAAAC 
CCAATGCCAG CTCCTGTTGA GCATCAGATC TGTCCTGCTG TGTGTGTTCT AATGAAGCTT 
TCATTTGATG AAGAGCATAG GCATGCAATG AATGAACTTG GGGGACTGCA GGCCATTGCA 
GAGTTATTGC AGGTGGACTG TGAGATGTAT GGGCTTACTA ATGACCACTA CAGTGTTACT 
TTAAGACGGT ATGCTGGAAT GGCTTTGACA AACTTGACCT TTGGAGATGT TGCCAACAAG 
GCTACGCTGT GTTCTATGAA AGGCTGCATG AGAGCACTTG TGGCCCAGTT AAAATCTGAG 
AGTGAAGACT TACAGCAGGT TATTGCAAGT GTTTTGAGGA ATTTGTCTTG GCGAGCAGAT 
GTAAATAGCA AAAAGACGTT GAGAGAAGTT GGAAGTGTGA AAGCATTGAT GGAATGTGCT 
TTGGAAGTTA AAAAGGAATC AACCCTCAAA AGCGTTTTGA GTGCCTTATG GAACCTGTCT 
GCACACTGCA CTGAGAATAA GGCTGACATC TGTGCTGTGG ATGGAGCACT GGCATTTCTG 
GTTGGCACCC TCACTTACCG GAGCCAGACA AATACTTTAG CCATTATTGA AAGTGGAGGT 
GGGATATTAC GGAATGTGTC CAGCTTGATA GCTACAAACG AAGACCACAG GCAAATCCTA 
AGAGAGAACA ATTGCCTACA AACTTTATTA CAGCACTTGA AATCTCACAG CTTGACAATA 
GTCAGTAATG CATGTGGAAC TTTGTGGAAT CTCTCAGCAA GAAATCCTAA AGACCAGGAA 
GCCTTGTGGG ACATGGGGGC AGTGAGCATG CTCAAGAACC TCATTCATTC CAAGCACAAA 
ATGATTGCCA TGGGAAGTGC AGCAGCTTTA AGGAATCTCA TGGCAAACAG ACCTGCAAAG 
TATAAGGATG CCAATATCAT GTCTCCCGGC TCAAGTCTGC CATCCCTTCA CGTTAGGAAA 
CAGAAAGCTC TAGAAGCTGA GCTAGATGCT CAGCATTTAT CAGAAACCTT CGACAACATT 
GACAACCTAA GTCCCAAGGC CTCTCACCGG AGTAAGCAGA GACACAAGCA GAATCTTTAT 
GGTGACTATG CTTTTGACGC CAATCGACAT GATGATAGTA GGTCAGACAA TTTCAATACT 
GGAAACATGA CTGTTCTTTC ACCATATTTA AATACTACGG TATTGCCCAG CTCTTCTTCC 
TCAAGGGGAA GTTTAGACAG TTCTCGTTCT GAGAAAGACA GAAGTTTGGA GAGAGAGCGA 
GGTATTGGCC TCAGTGCTTA CCATCCAACA ACAGAAAATG CAGGAACCTC ATCAAAACGA 
GGTCTGCAGA TCACTACCAC TGCAGCCCAG ATAGCCAAAG TTATGGAAGA AGTATCAGCC 
ATTCATACCT CCCAGGACGA CAGAAGTTCT GCTTCTACCA CCGAGTTCCA TTGTGTGGCA 
GACGACAGGA GTGCGGCACG AAGAAGCTCT GCCTCCCACA CACACTCAAA CACATACAAC 
TTCACTAAGT CGGAAAATTC AAATAGGACA TGCTCTATGC CTTATGCCAA AGTGGAATAT 
AAACGATCTT CAAATGACAG TTTAAATAGT GTCACTAGTA GTGATGGATA TGGTAAAAGA 
GGCCAAATGA AACCCTCAGT TGAATCCTAT TCTGAAGATG ATGAAAGTAA ATTTTGCAGT 
TATGGTCAGT ATCCAGCTGA CCTAGCCCAT AAGATACACA GTGCAAATCA TATGGATGAT 
AATGATGGAG AACTGGATAC ACCAATAAAT TACAGTCTTA AATATTCAGA TGAGCAGTTG 
AACTCAGGAA GGCAGAGTCC CTCACAGAAT GAAAGGTGGG CAAGACCAAA GCATGTGATA 
GAAGATGAAA TAAAGCAAAA CGAGCAAAGA CAAGCAAGAA GCCAGAACAC CAGTTATCCT 
GTCTATTCTG AGAATACCGA TGACAAACAC CTCAAATTCC AACCACATTT TGGACAACAA 
GAATGTGTTT CCCCATATAG GTCAAGGGGA ACCAGTGGTT CAGAAACAAA TCGAATGGGT 
TCTAGTCATG CAATTAATCA AAATGTAAAC CAGTCTCTGT GTCAGGAAGA TGATTATGAA 
GATGATAAAC CTACCAACTA CAGTGAACGT TATTCTGAGG AAGAACAACA TGAAGAAGAA 
GAAGAGAGAC CGACAAATTA TAGCATAAAA TATAATGAAG AGAAACATCA TGTGGATCAG 
CCTATTGATT ATAGTTTAAA ATATGCCACT GACATTTCTT CCTCACAAAA ACCATCATTT 
TCATTCTCAA AGAATTCATC AGCACAAAGC ACTAAACCTG AACATCTCTC TCCAAGCAGC 
GAGAATACAG CTGTACCTCC ATCTAATGCC AAAAGGCAGA ATCAGCTGCG TCCAAGTTCA 
GCACAAAGAA ATGGCCAGAC TCAAAAAGGC ACTACTTGCA AAGTCCCCTC CATCAACCAA 
GAAACAATAC AGACTTACTG CGTAGAAGAC ACCCCAATAT GTTTTTCAAG GTGCAGTTCA 
TTATCATCAC TGTCATCAGC TGACGATGAA ATAGGATGTG ATCAGACAAC ACAGGAAGCA 
GATTCTGCTA ATACTCTGCA GACAGCAGAA GTAAAAGAGA ATGATGTAAC TCGGTCAGCT 
GAAGATCCTG CAACTGAAGT TCCAGCAGTG TCCCAGAATG CTAGAGCCAA ACCCAGCCGA 
CTCCAGGCTT CTGGCTTATC TTCAGAATCA ACCAGGCATA ATAAAGCTGT TGAGTTTTCT 
TCAGGAGCCA AGTCTCCCTC CAAAAGTGGT GCTCAGACAC CCAAAAGTCC CCCAGAACAC 
TATGTCCAGG AGACTCCGCT CGTATTCAGC AGGTGTACTT CTGTCAGCTC CCTTGACAGT 
TTTGAGAGTC GCTCCATTGC CAGCTCTGTT CAGAGTGAGC CATGTAGTGG AATGGTGAGT 
GGCATCATAA GCCCCAGTGA CCTTCCAGAT AGTCCTGGGC AGACCATGCC ACCAAGCAGA 
AGCAAAACCC CTCCACCTCC TCCACAGACA GTGCAGGCCA AGAGAGAGGT GCCAAAAAGT 
AAAGTCCCTG CTGCTGAGAA GAGAGAGAGT GGGCCTAAGC AGACTGCTGT AAATGCTGCC 
GTGCAGAGGG TGCAGGTCCT TCCAGACGTG GATACTTTGT TACACTTCGC CACAGAAAGT 
ACTCCAGACG GGTTTTCTTG TTCCTCCAGC CTAAGTGCTC TGAGCCTGGA TGAGCCATTT 
ATACAGAAAG ATGTAGAATT AAGAATCATG CCTCCAGTTC AGGAAAACGA CAATGGGAAT 
GAAACTGAAT CAGAACAGCC TGAGGAATCA AATGAAAACC AGGATAAAGA GGTAGAAAAG 
CCTGACTCTG AAAAAGACTT ATTAGATGAT TCTGATGACG ATGATATTGA AATATTAGAA 
GAATGTATTA TTTCAGCCAT GCCAACAAAG TCATCACGCA AAGCCAAAAA ACTAGCCCAG 
ACTGCTTCAA AATTACCTCC ACCTGTGGCA AGGAAACCAA GTCAGCTACC TGTGTATAAA 
CTTCTGCCAG CACAGAATAG GCTGCAGGCA CAAAAACATG TTAGCTTTAC ACCAGGGGAT 
GATGTGCCCC GGGTGTACTG TGTAGAAGGG ACACCTATAA ACTTTTCCAC AGCAACGTCT 
CTAAGTGATC TGACAATAGA GTCCCCTCCA AATGAATTGG CTACTGGAGA TGGGGTCAGA 
GCGGGTATAC AGTCAGGTGA ATTTGAAAAA CGAGATACCA TTCCTACAGA AGGCAGAAGT 
ACAGATGATG CTCAGCGAGG AAAAATCTCA TCTATAGTTA CACCAGACCT GGATGACAAC 
AAAGCAGAGG AAGGAGATAT TCTTGCAGAA TGTATCAATT CTGCTATGCC CAAAGGAAAA 
AGCCACAAGC CTTTCCGAGT GAAAAAGATA ATGGACCAAG TCCAACAAGC ATCCTCGACT 
TCATCTGGAG CTAACAAAAA TCAAGTAGAC ACTAAGAAAA AGAAGCCTAC TTCACCAGTA 
AAGCCCATGC CACAAAATAC TGAATATAGA ACGCGTGTGA GAAAGAATAC AGACTCAAAA 
GTTAATGTAA ATACTGAAGA AACTTTCTCA GACAACAAAG ACTCAAAGAA ACCAAGCTTA 
CAAACCAATG CCAAGGCCTT CAATGAAAAG CTACCTAACA ATGAAGACAG AGTGCGGGGG 
AGCTTCGCCT TGGACTCACC GCATCACTAC ACCCCTATTG AGGGGACGCC GTACTGCTTT 
TCCCGAAATG ACTCCTTGAG TTCTCTGGAT TTTGATGATG ACGATGTTGA CCTTTCCAGG 
GAAAAGGCCG AGTTAAGAAA GGGCAAAGAA AGCAAGGATT CCGAAGCCAA AGTTACCTGC 
CGCCCAGAAC CAAACTCAAG CCAGCAGGCA GCTAGTAAGT CACAAGCCAG TATAAAACAT 
CCAGCAAACA GAGCACAGTC CAAACCAGTG CTGCAGAAAC AGCCCACTTT CCCCCAGTCC 
TCCAAAGACG GACCAGATAG AGGGGCAGCA ACTGACGAAA AACTGCAGAA TTTTGCTATT 
GAAAATACTC CAGTTTGCTT TTCTCGAAAT TCCTCTCTGA GTTCCCTTAG TGACATTGAC 
CAGGAAAACA ACAATAACAA AGAAAGTGAA CCAATCAAAG AAGCTGAACC TGCCAACTCA 
CAAGGAGAGC CCAGTAAGCC TCAGGCATCC GGGTATGCTC CCAAGTCCTT CCACGTCGAA 
GACACCCCTG TCTGTTTCTC AAGAAACAGC TCTCTCAGTT CTCTTAGCAT TGACTCTGAG 
GACGACCTGT TACAGGAGTG TATAAGTTCT GCCATGCCAA AAAAGAAAAG GCCTTCAAGA 
CTCAAGAGTG AGAGCGAAAA GCAGAGCCCT AGAAAAGTGG GTGGCATATT AGCTGAAGAC 
CTGACGCTTG ATTTGAAAGA TCTACAGAGG CCAGATTCAG AACACGCTTT CTCCCCCGAC 
TCAGAAAATT TTGACTGGAA AGCTATTCAG GAAGGCGCAA ACTCCATAGT AAGTAGTTTG 
CACCAAGCTG CTGCAGCCGC CGCGTGCTTA TCTAGACAAG CGTCATCCGA CTCAGATTCC 
ATTCTGTCAC TAAAGTCCGG CATTTCTCTG GGATCGCCTT TTCATCTTAC ACCTGATCAA 
GAGGAAAAGC CATTCACAAG CAATAAAGGC CCAAGAATTC TCAAACCTGG AGAGAAAAGC 
ACATTAGAAG CAAAAAAAAT AGAATCTGAA AACAAAGGAA TCAAAGGCGG GAAAAAGGTT 
TATAAAAGCT TGATTACGGG AAAGATTCGC TCCAATTCAG AAATTTCCAG CCAAATGAAA 
CAACCCCTCC CGACAAACAT GCCTTCAATC TCAAGAGGCA GGACGATGAT TCACATCCCA 
GGGCTTCGGA ATAGCTCCTC TAGTACAAGC CCTGTCTCTA AGAAAGGCCC ACCCCTCAAG 
ACTCCAGCCT CTAAAAGCCC CAGTGAAGGG CCGGGAGCTA CCACTTCTCC TCGAGGAACT 
AAGCCAGCAG GAAAGTCAGA GCTTAGCCCT ATCACCAGGC AAACTTCCCA AATCAGTGGG 
TCAAATAAGG GGTCTTCTAG ATCAGGATCT AGAGACTCCA CTCCCTCAAG ACCTACACAG 
CAACCATTAA GTAGGCCAAT GCAGTCTCCA GGGCGAAACT CAATTTCCCC TGGTAGAAAT 
GGAATAAGCC CTCCTAACAA ACTGTCTCAG CTGCCCAGAA CATCATCTCC CAGTACTGCT 
TCAACTAAGT CCTCCGGTTC TGGGAAAATG TCATATACAT CCCCAGGTAG ACAGCTGAGC 
CAACAAAATC TTACCAAACA AGCAAGTTTA TCCAAGAATG CCAGCAGTAT CCCCAGAAGT 
GAGTCGGCAT CTAAAGGACT GAATCAGATG AGTAACGGCA ATGGGTCAAA TAAAAAGGTA 
GAACTTTCTA GAATGTCTTC AACTAAATCA AGTGGAAGTG AATCAGACAG ATCAGAAAGG 
CCTGCATTAG TACGCCAGTC TACTTTCATC AAAGAAGCCC CAAGCCCAAC CCTGAGGAGG 
AAACTGGAGG AATCTGCCTC ATTTGAATCC CTTTCTCCAT CTTCTAGACC AGATTCTCCC 
ACCAGGTCGC AGGCACAGAC CCCAGTTTTA AGCCCTTCCC TTCCTGATAT GTCTCTGTCC 
ACACATCCAT CTGTTCAGGC AGGTGGGTGG CGAAAGCTCC CGCCTAATCT CAGCCCCACT 
ATCGAGTATA ATGACGGAAG GCCCACAAAA CGGCATGATA TTGCACGCTC CCATTCTGAA 
AGTCCTTCCA GACTACCAAT CAACCGGGCG GGAACCTGGA AGCGTGAACA CAGCAAACAT 
TCCTCGTCCC TTCCTCGAGT GAGTACTTGG AGAAGAACTG GAAGCTCATC TTCTATTCTT 
TCTGCTTCAT CAGAGTCCAG TGAAAAAGCA AAAAGTGAGG ATGAAAGGCA TGTGAGCTCC 
ATGCCAGCAC CCAGACAGAT GAAGGAAAAC CAGGTGCCCA CCAAAGGAAC ATGGAGGAAA 
ATCAAGGAAA GTGACATTTC TCCCACAGGC ATGGCTTCTC AGAGCGCTTC CTCAGGTGCT 
GCCAGTGGTG CTGAATCCAA GCCTCTGATC TATCAGATGG CACCTCCTGT CTCTAAAACA 
GAGGATGTTT GGGTGAGAAT TGAGGACTGC CCCATTAACA ACCCTAGATC TGGACGGTCC 
CCCACAGGCA ACACCCCCCC AGTGATTGAC AGTGTTTCAG AGAAGGGAAG TTCAAGCATT 
AAAGATTCAA AAGACACCCA TGGGAAACAG AGTGTGGGCA GTGGCAGTCC TGTGCAAACC 
GTGGGTCTGG AAACCCGCCT CAACTCCTTT GTTCAGGTAG AGGCCCCAGA ACAGAAAGGA 
ACTGAGGCAA AACCAGGACA GAGTAACCCA GTCTCTATAG CAGAGACTGC TGAGACGTGT 
ATAGCAGAGC GTACCCCTTT CAGTTCCAGT AGCTCCAGCA AGCACAGCTC ACCTAGCGGG 
ACTGTTGCTG CCAGAGTGAC ACCTTTTAAT TACAACCCTA GCCCTAGGAA GAGCAGCGCA 
GACAGCACTT CAGCCCGGCC GTCTCAGATC CCTACGCCAG TGAGCACCAA CACGAAGAAG 
AGAGATTCGA AGACTGACAG CACAGAATCC AGTGGAGCCC AAAGTCCTAA ACGCCATTCC 
GGGTCTTACC TCGTGACGTC TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106152.1
CDS	294..8540
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAESPATRVDHETASVLSSSGTHSAPRRLTSHLGTKVEMVYSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250292.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250292.1

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGTC CAGCTACACG TGTGGATCAC GAAACAGCCA GTGTTTTGAG TTCTAGCGGC 
ACGCACTCTG CTCCTCGAAG GTTGACAAGT CATCTGGGGA CAAAGGTGGA AATGGTGTAT 
TCCTTGTTGT CAATGCTTGG TACTCATGAT AAGGACGATA TGTCACGAAC TTTGCTAGCT 
ATGTCCAGCT CCCAAGACAG CTGTATATCC ATGCGGCAGT CTGGATGTCT TCCTCTCCTC 
ATCCAGCTTT TACATGGCAA TGACAAAGAC TCTGTATTGT TGGGAAATTC CCGGGGCAGT 
AAAGAGGCTC GGGCCAGGGC CAGTGCAGCA CTCCACAACA TCATTCACTC ACAGCCTGAT 
GACAAGAGAG GCAGGCGTGA AATCCGAGTC CTTCATCTTT TGGAACAGAT ACGAGCTTAC 
TGTGAAACCT GTTGGGAGTG GCAGGAAGCC CACGAACAAG GCATGGACCA GGACAAAAAC 
CCAATGCCAG CTCCTGTTGA GCATCAGATC TGTCCTGCTG TGTGTGTTCT AATGAAGCTT 
TCATTTGATG AAGAGCATAG GCATGCAATG AATGAACTTG GGGGACTGCA GGCCATTGCA 
GAGTTATTGC AGGTGGACTG TGAGATGTAT GGGCTTACTA ATGACCACTA CAGTGTTACT 
TTAAGACGGT ATGCTGGAAT GGCTTTGACA AACTTGACCT TTGGAGATGT TGCCAACAAG 
GCTACGCTGT GTTCTATGAA AGGCTGCATG AGAGCACTTG TGGCCCAGTT AAAATCTGAG 
AGTGAAGACT TACAGCAGGT TATTGCAAGT GTTTTGAGGA ATTTGTCTTG GCGAGCAGAT 
GTAAATAGCA AAAAGACGTT GAGAGAAGTT GGAAGTGTGA AAGCATTGAT GGAATGTGCT 
TTGGAAGTTA AAAAGGAATC AACCCTCAAA AGCGTTTTGA GTGCCTTATG GAACCTGTCT 
GCACACTGCA CTGAGAATAA GGCTGACATC TGTGCTGTGG ATGGAGCACT GGCATTTCTG 
GTTGGCACCC TCACTTACCG GAGCCAGACA AATACTTTAG CCATTATTGA AAGTGGAGGT 
GGGATATTAC GGAATGTGTC CAGCTTGATA GCTACAAACG AAGACCACAG GCAAATCCTA 
AGAGAGAACA ATTGCCTACA AACTTTATTA CAGCACTTGA AATCTCACAG CTTGACAATA 
GTCAGTAATG CATGTGGAAC TTTGTGGAAT CTCTCAGCAA GAAATCCTAA AGACCAGGAA 
GCCTTGTGGG ACATGGGGGC AGTGAGCATG CTCAAGAACC TCATTCATTC CAAGCACAAA 
ATGATTGCCA TGGGAAGTGC AGCAGCTTTA AGGAATCTCA TGGCAAACAG ACCTGCAAAG 
TATAAGGATG CCAATATCAT GTCTCCCGGC TCAAGTCTGC CATCCCTTCA CGTTAGGAAA 
CAGAAAGCTC TAGAAGCTGA GCTAGATGCT CAGCATTTAT CAGAAACCTT CGACAACATT 
GACAACCTAA GTCCCAAGGC CTCTCACCGG AGTAAGCAGA GACACAAGCA GAATCTTTAT 
GGTGACTATG CTTTTGACGC CAATCGACAT GATGATAGTA GGTCAGACAA TTTCAATACT 
GGAAACATGA CTGTTCTTTC ACCATATTTA AATACTACGG TATTGCCCAG CTCTTCTTCC 
TCAAGGGGAA GTTTAGACAG TTCTCGTTCT GAGAAAGACA GAAGTTTGGA GAGAGAGCGA 
GGTATTGGCC TCAGTGCTTA CCATCCAACA ACAGAAAATG CAGGAACCTC ATCAAAACGA 
GGTCTGCAGA TCACTACCAC TGCAGCCCAG ATAGCCAAAG TTATGGAAGA AGTATCAGCC 
ATTCATACCT CCCAGGACGA CAGAAGTTCT GCTTCTACCA CCGAGTTCCA TTGTGTGGCA 
GACGACAGGA GTGCGGCACG AAGAAGCTCT GCCTCCCACA CACACTCAAA CACATACAAC 
TTCACTAAGT CGGAAAATTC AAATAGGACA TGCTCTATGC CTTATGCCAA AGTGGAATAT 
AAACGATCTT CAAATGACAG TTTAAATAGT GTCACTAGTA GTGATGGATA TGGTAAAAGA 
GGCCAAATGA AACCCTCAGT TGAATCCTAT TCTGAAGATG ATGAAAGTAA ATTTTGCAGT 
TATGGTCAGT ATCCAGCTGA CCTAGCCCAT AAGATACACA GTGCAAATCA TATGGATGAT 
AATGATGGAG AACTGGATAC ACCAATAAAT TACAGTCTTA AATATTCAGA TGAGCAGTTG 
AACTCAGGAA GGCAGAGTCC CTCACAGAAT GAAAGGTGGG CAAGACCAAA GCATGTGATA 
GAAGATGAAA TAAAGCAAAA CGAGCAAAGA CAAGCAAGAA GCCAGAACAC CAGTTATCCT 
GTCTATTCTG AGAATACCGA TGACAAACAC CTCAAATTCC AACCACATTT TGGACAACAA 
GAATGTGTTT CCCCATATAG GTCAAGGGGA ACCAGTGGTT CAGAAACAAA TCGAATGGGT 
TCTAGTCATG CAATTAATCA AAATGTAAAC CAGTCTCTGT GTCAGGAAGA TGATTATGAA 
GATGATAAAC CTACCAACTA CAGTGAACGT TATTCTGAGG AAGAACAACA TGAAGAAGAA 
GAAGAGAGAC CGACAAATTA TAGCATAAAA TATAATGAAG AGAAACATCA TGTGGATCAG 
CCTATTGATT ATAGTTTAAA ATATGCCACT GACATTTCTT CCTCACAAAA ACCATCATTT 
TCATTCTCAA AGAATTCATC AGCACAAAGC ACTAAACCTG AACATCTCTC TCCAAGCAGC 
GAGAATACAG CTGTACCTCC ATCTAATGCC AAAAGGCAGA ATCAGCTGCG TCCAAGTTCA 
GCACAAAGAA ATGGCCAGAC TCAAAAAGGC ACTACTTGCA AAGTCCCCTC CATCAACCAA 
GAAACAATAC AGACTTACTG CGTAGAAGAC ACCCCAATAT GTTTTTCAAG GTGCAGTTCA 
TTATCATCAC TGTCATCAGC TGACGATGAA ATAGGATGTG ATCAGACAAC ACAGGAAGCA 
GATTCTGCTA ATACTCTGCA GACAGCAGAA GTAAAAGAGA ATGATGTAAC TCGGTCAGCT 
GAAGATCCTG CAACTGAAGT TCCAGCAGTG TCCCAGAATG CTAGAGCCAA ACCCAGCCGA 
CTCCAGGCTT CTGGCTTATC TTCAGAATCA ACCAGGCATA ATAAAGCTGT TGAGTTTTCT 
TCAGGAGCCA AGTCTCCCTC CAAAAGTGGT GCTCAGACAC CCAAAAGTCC CCCAGAACAC 
TATGTCCAGG AGACTCCGCT CGTATTCAGC AGGTGTACTT CTGTCAGCTC CCTTGACAGT 
TTTGAGAGTC GCTCCATTGC CAGCTCTGTT CAGAGTGAGC CATGTAGTGG AATGGTGAGT 
GGCATCATAA GCCCCAGTGA CCTTCCAGAT AGTCCTGGGC AGACCATGCC ACCAAGCAGA 
AGCAAAACCC CTCCACCTCC TCCACAGACA GTGCAGGCCA AGAGAGAGGT GCCAAAAAGT 
AAAGTCCCTG CTGCTGAGAA GAGAGAGAGT GGGCCTAAGC AGACTGCTGT AAATGCTGCC 
GTGCAGAGGG TGCAGGTCCT TCCAGACGTG GATACTTTGT TACACTTCGC CACAGAAAGT 
ACTCCAGACG GGTTTTCTTG TTCCTCCAGC CTAAGTGCTC TGAGCCTGGA TGAGCCATTT 
ATACAGAAAG ATGTAGAATT AAGAATCATG CCTCCAGTTC AGGAAAACGA CAATGGGAAT 
GAAACTGAAT CAGAACAGCC TGAGGAATCA AATGAAAACC AGGATAAAGA GGTAGAAAAG 
CCTGACTCTG AAAAAGACTT ATTAGATGAT TCTGATGACG ATGATATTGA AATATTAGAA 
GAATGTATTA TTTCAGCCAT GCCAACAAAG TCATCACGCA AAGCCAAAAA ACTAGCCCAG 
ACTGCTTCAA AATTACCTCC ACCTGTGGCA AGGAAACCAA GTCAGCTACC TGTGTATAAA 
CTTCTGCCAG CACAGAATAG GCTGCAGGCA CAAAAACATG TTAGCTTTAC ACCAGGGGAT 
GATGTGCCCC GGGTGTACTG TGTAGAAGGG ACACCTATAA ACTTTTCCAC AGCAACGTCT 
CTAAGTGATC TGACAATAGA GTCCCCTCCA AATGAATTGG CTACTGGAGA TGGGGTCAGA 
GCGGGTATAC AGTCAGGTGA ATTTGAAAAA CGAGATACCA TTCCTACAGA AGGCAGAAGT 
ACAGATGATG CTCAGCGAGG AAAAATCTCA TCTATAGTTA CACCAGACCT GGATGACAAC 
AAAGCAGAGG AAGGAGATAT TCTTGCAGAA TGTATCAATT CTGCTATGCC CAAAGGAAAA 
AGCCACAAGC CTTTCCGAGT GAAAAAGATA ATGGACCAAG TCCAACAAGC ATCCTCGACT 
TCATCTGGAG CTAACAAAAA TCAAGTAGAC ACTAAGAAAA AGAAGCCTAC TTCACCAGTA 
AAGCCCATGC CACAAAATAC TGAATATAGA ACGCGTGTGA GAAAGAATAC AGACTCAAAA 
GTTAATGTAA ATACTGAAGA AACTTTCTCA GACAACAAAG ACTCAAAGAA ACCAAGCTTA 
CAAACCAATG CCAAGGCCTT CAATGAAAAG CTACCTAACA ATGAAGACAG AGTGCGGGGG 
AGCTTCGCCT TGGACTCACC GCATCACTAC ACCCCTATTG AGGGGACGCC GTACTGCTTT 
TCCCGAAATG ACTCCTTGAG TTCTCTGGAT TTTGATGATG ACGATGTTGA CCTTTCCAGG 
GAAAAGGCCG AGTTAAGAAA GGGCAAAGAA AGCAAGGATT CCGAAGCCAA AGTTACCTGC 
CGCCCAGAAC CAAACTCAAG CCAGCAGGCA GCTAGTAAGT CACAAGCCAG TATAAAACAT 
CCAGCAAACA GAGCACAGTC CAAACCAGTG CTGCAGAAAC AGCCCACTTT CCCCCAGTCC 
TCCAAAGACG GACCAGATAG AGGGGCAGCA ACTGACGAAA AACTGCAGAA TTTTGCTATT 
GAAAATACTC CAGTTTGCTT TTCTCGAAAT TCCTCTCTGA GTTCCCTTAG TGACATTGAC 
CAGGAAAACA ACAATAACAA AGAAAGTGAA CCAATCAAAG AAGCTGAACC TGCCAACTCA 
CAAGGAGAGC CCAGTAAGCC TCAGGCATCC GGGTATGCTC CCAAGTCCTT CCACGTCGAA 
GACACCCCTG TCTGTTTCTC AAGAAACAGC TCTCTCAGTT CTCTTAGCAT TGACTCTGAG 
GACGACCTGT TACAGGAGTG TATAAGTTCT GCCATGCCAA AAAAGAAAAG GCCTTCAAGA 
CTCAAGAGTG AGAGCGAAAA GCAGAGCCCT AGAAAAGTGG GTGGCATATT AGCTGAAGAC 
CTGACGCTTG ATTTGAAAGA TCTACAGAGG CCAGATTCAG AACACGCTTT CTCCCCCGAC 
TCAGAAAATT TTGACTGGAA AGCTATTCAG GAAGGCGCAA ACTCCATAGT AAGTAGTTTG 
CACCAAGCTG CTGCAGCCGC CGCGTGCTTA TCTAGACAAG CGTCATCCGA CTCAGATTCC 
ATTCTGTCAC TAAAGTCCGG CATTTCTCTG GGATCGCCTT TTCATCTTAC ACCTGATCAA 
GAGGAAAAGC CATTCACAAG CAATAAAGGC CCAAGAATTC TCAAACCTGG AGAGAAAAGC 
ACATTAGAAG CAAAAAAAAT AGAATCTGAA AACAAAGGAA TCAAAGGCGG GAAAAAGGTT 
TATAAAAGCT TGATTACGGG AAAGATTCGC TCCAATTCAG AAATTTCCAG CCAAATGAAA 
CAACCCCTCC CGACAAACAT GCCTTCAATC TCAAGAGGCA GGACGATGAT TCACATCCCA 
GGGCTTCGGA ATAGCTCCTC TAGTACAAGC CCTGTCTCTA AGAAAGGCCC ACCCCTCAAG 
ACTCCAGCCT CTAAAAGCCC CAGTGAAGGG CCGGGAGCTA CCACTTCTCC TCGAGGAACT 
AAGCCAGCAG GAAAGTCAGA GCTTAGCCCT ATCACCAGGC AAACTTCCCA AATCAGTGGG 
TCAAATAAGG GGTCTTCTAG ATCAGGATCT AGAGACTCCA CTCCCTCAAG ACCTACACAG 
CAACCATTAA GTAGGCCAAT GCAGTCTCCA GGGCGAAACT CAATTTCCCC TGGTAGAAAT 
GGAATAAGCC CTCCTAACAA ACTGTCTCAG CTGCCCAGAA CATCATCTCC CAGTACTGCT 
TCAACTAAGT CCTCCGGTTC TGGGAAAATG TCATATACAT CCCCAGGTAG ACAGCTGAGC 
CAACAAAATC TTACCAAACA AGCAAGTTTA TCCAAGAATG CCAGCAGTAT CCCCAGAAGT 
GAGTCGGCAT CTAAAGGACT GAATCAGATG AGTAACGGCA ATGGGTCAAA TAAAAAGGTA 
GAACTTTCTA GAATGTCTTC AACTAAATCA AGTGGAAGTG AATCAGACAG ATCAGAAAGG 
CCTGCATTAG TACGCCAGTC TACTTTCATC AAAGAAGCCC CAAGCCCAAC CCTGAGGAGG 
AAACTGGAGG AATCTGCCTC ATTTGAATCC CTTTCTCCAT CTTCTAGACC AGATTCTCCC 
ACCAGGTCGC AGGCACAGAC CCCAGTTTTA AGCCCTTCCC TTCCTGATAT GTCTCTGTCC 
ACACATCCAT CTGTTCAGGC AGGTGGGTGG CGAAAGCTCC CGCCTAATCT CAGCCCCACT 
ATCGAGTATA ATGACGGAAG GCCCACAAAA CGGCATGATA TTGCACGCTC CCATTCTGAA 
AGTCCTTCCA GACTACCAAT CAACCGGGCG GGAACCTGGA AGCGTGAACA CAGCAAACAT 
TCCTCGTCCC TTCCTCGAGT GAGTACTTGG AGAAGAACTG GAAGCTCATC TTCTATTCTT 
TCTGCTTCAT CAGAGTCCAG TGAAAAAGCA AAAAGTGAGG ATGAAAGGCA TGTGAGCTCC 
ATGCCAGCAC CCAGACAGAT GAAGGAAAAC CAGGTGCCCA CCAAAGGAAC ATGGAGGAAA 
ATCAAGGAAA GTGACATTTC TCCCACAGGC ATGGCTTCTC AGAGCGCTTC CTCAGGTGCT 
GCCAGTGGTG CTGAATCCAA GCCTCTGATC TATCAGATGG CACCTCCTGT CTCTAAAACA 
GAGGATGTTT GGGTGAGAAT TGAGGACTGC CCCATTAACA ACCCTAGATC TGGACGGTCC 
CCCACAGGCA ACACCCCCCC AGTGATTGAC AGTGTTTCAG AGAAGGGAAG TTCAAGCATT 
AAAGATTCAA AAGACACCCA TGGGAAACAG AGTGTGGGCA GTGGCAGTCC TGTGCAAACC 
GTGGGTCTGG AAACCCGCCT CAACTCCTTT GTTCAGGTAG AGGCCCCAGA ACAGAAAGGA 
ACTGAGGCAA AACCAGGACA GAGTAACCCA GTCTCTATAG CAGAGACTGC TGAGACGTGT 
ATAGCAGAGC GTACCCCTTT CAGTTCCAGT AGCTCCAGCA AGCACAGCTC ACCTAGCGGG 
ACTGTTGCTG CCAGAGTGAC ACCTTTTAAT TACAACCCTA GCCCTAGGAA GAGCAGCGCA 
GACAGCACTT CAGCCCGGCC GTCTCAGATC CCTACGCCAG TGAGCACCAA CACGAAGAAG 
AGAGATTCGA AGACTGACAG CACAGAATCC AGTGGAGCCC AAAGTCCTAA ACGCCATTCC 
GGGTCTTACC TCGTGACGTC TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250293.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106153.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X25, mRNA.

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CloneID	OMu87331
Clone ID Related Accession (Same CDS sequence)	XM_030250293.1
Accession Version	XM_030250293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGTCCAG CTACACGTGT GGATCACGAA ACAGCCAGTG TTTTGAGTTC TAGCGGCACG 
CACTCTGCTC CTCGAAGGTT GACAAGTCAT CTGGGGACAA AGATACGAGC TTACTGTGAA 
ACCTGTTGGG AGTGGCAGGA AGCCCACGAA CAAGGCATGG ACCAGGACAA AAACCCAATG 
CCAGCTCCTG TTGAGCATCA GATCTGTCCT GCTGTGTGTG TTCTAATGAA GCTTTCATTT 
GATGAAGAGC ATAGGCATGC AATGAATGAA CTTGGTAGGA AGGCTACCCG GGGCATTTCA 
TCCCAGGAGC TAGGTCAGGG GCTTTCAGGG GGACTGCAGG CCATTGCAGA GTTATTGCAG 
GTGGACTGTG AGATGTATGG GCTTACTAAT GACCACTACA GTGTTACTTT AAGACGGTAT 
GCTGGAATGG CTTTGACAAA CTTGACCTTT GGAGATGTTG CCAACAAGGC TACGCTGTGT 
TCTATGAAAG GCTGCATGAG AGCACTTGTG GCCCAGTTAA AATCTGAGAG TGAAGACTTA 
CAGCAGGTTA TTGCAAGTGT TTTGAGGAAT TTGTCTTGGC GAGCAGATGT AAATAGCAAA 
AAGACGTTGA GAGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTTAAA 
AAGGAATCAA CCCTCAAAAG CGTTTTGAGT GCCTTATGGA ACCTGTCTGC ACACTGCACT 
GAGAATAAGG CTGACATCTG TGCTGTGGAT GGAGCACTGG CATTTCTGGT TGGCACCCTC 
ACTTACCGGA GCCAGACAAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG GATATTACGG 
AATGTGTCCA GCTTGATAGC TACAAACGAA GACCACAGGC AAATCCTAAG AGAGAACAAT 
TGCCTACAAA CTTTATTACA GCACTTGAAA TCTCACAGCT TGACAATAGT CAGTAATGCA 
TGTGGAACTT TGTGGAATCT CTCAGCAAGA AATCCTAAAG ACCAGGAAGC CTTGTGGGAC 
ATGGGGGCAG TGAGCATGCT CAAGAACCTC ATTCATTCCA AGCACAAAAT GATTGCCATG 
GGAAGTGCAG CAGCTTTAAG GAATCTCATG GCAAACAGAC CTGCAAAGTA TAAGGATGCC 
AATATCATGT CTCCCGGCTC AAGTCTGCCA TCCCTTCACG TTAGGAAACA GAAAGCTCTA 
GAAGCTGAGC TAGATGCTCA GCATTTATCA GAAACCTTCG ACAACATTGA CAACCTAAGT 
CCCAAGGCCT CTCACCGGAG TAAGCAGAGA CACAAGCAGA ATCTTTATGG TGACTATGCT 
TTTGACGCCA ATCGACATGA TGATAGTAGG TCAGACAATT TCAATACTGG AAACATGACT 
GTTCTTTCAC CATATTTAAA TACTACGGTA TTGCCCAGCT CTTCTTCCTC AAGGGGAAGT 
TTAGACAGTT CTCGTTCTGA GAAAGACAGA AGTTTGGAGA GAGAGCGAGG TATTGGCCTC 
AGTGCTTACC ATCCAACAAC AGAAAATGCA GGAACCTCAT CAAAACGAGG TCTGCAGATC 
ACTACCACTG CAGCCCAGAT AGCCAAAGTT ATGGAAGAAG TATCAGCCAT TCATACCTCC 
CAGGACGACA GAAGTTCTGC TTCTACCACC GAGTTCCATT GTGTGGCAGA CGACAGGAGT 
GCGGCACGAA GAAGCTCTGC CTCCCACACA CACTCAAACA CATACAACTT CACTAAGTCG 
GAAAATTCAA ATAGGACATG CTCTATGCCT TATGCCAAAG TGGAATATAA ACGATCTTCA 
AATGACAGTT TAAATAGTGT CACTAGTAGT GATGGATATG GTAAAAGAGG CCAAATGAAA 
CCCTCAGTTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGTTA TGGTCAGTAT 
CCAGCTGACC TAGCCCATAA GATACACAGT GCAAATCATA TGGATGATAA TGATGGAGAA 
CTGGATACAC CAATAAATTA CAGTCTTAAA TATTCAGATG AGCAGTTGAA CTCAGGAAGG 
CAGAGTCCCT CACAGAATGA AAGGTGGGCA AGACCAAAGC ATGTGATAGA AGATGAAATA 
AAGCAAAACG AGCAAAGACA AGCAAGAAGC CAGAACACCA GTTATCCTGT CTATTCTGAG 
AATACCGATG ACAAACACCT CAAATTCCAA CCACATTTTG GACAACAAGA ATGTGTTTCC 
CCATATAGGT CAAGGGGAAC CAGTGGTTCA GAAACAAATC GAATGGGTTC TAGTCATGCA 
ATTAATCAAA ATGTAAACCA GTCTCTGTGT CAGGAAGATG ATTATGAAGA TGATAAACCT 
ACCAACTACA GTGAACGTTA TTCTGAGGAA GAACAACATG AAGAAGAAGA AGAGAGACCG 
ACAAATTATA GCATAAAATA TAATGAAGAG AAACATCATG TGGATCAGCC TATTGATTAT 
AGTTTAAAAT ATGCCACTGA CATTTCTTCC TCACAAAAAC CATCATTTTC ATTCTCAAAG 
AATTCATCAG CACAAAGCAC TAAACCTGAA CATCTCTCTC CAAGCAGCGA GAATACAGCT 
GTACCTCCAT CTAATGCCAA AAGGCAGAAT CAGCTGCGTC CAAGTTCAGC ACAAAGAAAT 
GGCCAGACTC AAAAAGGCAC TACTTGCAAA GTCCCCTCCA TCAACCAAGA AACAATACAG 
ACTTACTGCG TAGAAGACAC CCCAATATGT TTTTCAAGGT GCAGTTCATT ATCATCACTG 
TCATCAGCTG ACGATGAAAT AGGATGTGAT CAGACAACAC AGGAAGCAGA TTCTGCTAAT 
ACTCTGCAGA CAGCAGAAGT AAAAGAGAAT GATGTAACTC GGTCAGCTGA AGATCCTGCA 
ACTGAAGTTC CAGCAGTGTC CCAGAATGCT AGAGCCAAAC CCAGCCGACT CCAGGCTTCT 
GGCTTATCTT CAGAATCAAC CAGGCATAAT AAAGCTGTTG AGTTTTCTTC AGGAGCCAAG 
TCTCCCTCCA AAAGTGGTGC TCAGACACCC AAAAGTCCCC CAGAACACTA TGTCCAGGAG 
ACTCCGCTCG TATTCAGCAG GTGTACTTCT GTCAGCTCCC TTGACAGTTT TGAGAGTCGC 
TCCATTGCCA GCTCTGTTCA GAGTGAGCCA TGTAGTGGAA TGGTGAGTGG CATCATAAGC 
CCCAGTGACC TTCCAGATAG TCCTGGGCAG ACCATGCCAC CAAGCAGAAG CAAAACCCCT 
CCACCTCCTC CACAGACAGT GCAGGCCAAG AGAGAGGTGC CAAAAAGTAA AGTCCCTGCT 
GCTGAGAAGA GAGAGAGTGG GCCTAAGCAG ACTGCTGTAA ATGCTGCCGT GCAGAGGGTG 
CAGGTCCTTC CAGACGTGGA TACTTTGTTA CACTTCGCCA CAGAAAGTAC TCCAGACGGG 
TTTTCTTGTT CCTCCAGCCT AAGTGCTCTG AGCCTGGATG AGCCATTTAT ACAGAAAGAT 
GTAGAATTAA GAATCATGCC TCCAGTTCAG GAAAACGACA ATGGGAATGA AACTGAATCA 
GAACAGCCTG AGGAATCAAA TGAAAACCAG GATAAAGAGG TAGAAAAGCC TGACTCTGAA 
AAAGACTTAT TAGATGATTC TGATGACGAT GATATTGAAA TATTAGAAGA ATGTATTATT 
TCAGCCATGC CAACAAAGTC ATCACGCAAA GCCAAAAAAC TAGCCCAGAC TGCTTCAAAA 
TTACCTCCAC CTGTGGCAAG GAAACCAAGT CAGCTACCTG TGTATAAACT TCTGCCAGCA 
CAGAATAGGC TGCAGGCACA AAAACATGTT AGCTTTACAC CAGGGGATGA TGTGCCCCGG 
GTGTACTGTG TAGAAGGGAC ACCTATAAAC TTTTCCACAG CAACGTCTCT AAGTGATCTG 
ACAATAGAGT CCCCTCCAAA TGAATTGGCT ACTGGAGATG GGGTCAGAGC GGGTATACAG 
TCAGGTGAAT TTGAAAAACG AGATACCATT CCTACAGAAG GCAGAAGTAC AGATGATGCT 
CAGCGAGGAA AAATCTCATC TATAGTTACA CCAGACCTGG ATGACAACAA AGCAGAGGAA 
GGAGATATTC TTGCAGAATG TATCAATTCT GCTATGCCCA AAGGAAAAAG CCACAAGCCT 
TTCCGAGTGA AAAAGATAAT GGACCAAGTC CAACAAGCAT CCTCGACTTC ATCTGGAGCT 
AACAAAAATC AAGTAGACAC TAAGAAAAAG AAGCCTACTT CACCAGTAAA GCCCATGCCA 
CAAAATACTG AATATAGAAC GCGTGTGAGA AAGAATACAG ACTCAAAAGT TAATGTAAAT 
ACTGAAGAAA CTTTCTCAGA CAACAAAGAC TCAAAGAAAC CAAGCTTACA AACCAATGCC 
AAGGCCTTCA ATGAAAAGCT ACCTAACAAT GAAGACAGAG TGCGGGGGAG CTTCGCCTTG 
GACTCACCGC ATCACTACAC CCCTATTGAG GGGACGCCGT ACTGCTTTTC CCGAAATGAC 
TCCTTGAGTT CTCTGGATTT TGATGATGAC GATGTTGACC TTTCCAGGGA AAAGGCCGAG 
TTAAGAAAGG GCAAAGAAAG CAAGGATTCC GAAGCCAAAG TTACCTGCCG CCCAGAACCA 
AACTCAAGCC AGCAGGCAGC TAGTAAGTCA CAAGCCAGTA TAAAACATCC AGCAAACAGA 
GCACAGTCCA AACCAGTGCT GCAGAAACAG CCCACTTTCC CCCAGTCCTC CAAAGACGGA 
CCAGATAGAG GGGCAGCAAC TGACGAAAAA CTGCAGAATT TTGCTATTGA AAATACTCCA 
GTTTGCTTTT CTCGAAATTC CTCTCTGAGT TCCCTTAGTG ACATTGACCA GGAAAACAAC 
AATAACAAAG AAAGTGAACC AATCAAAGAA GCTGAACCTG CCAACTCACA AGGAGAGCCC 
AGTAAGCCTC AGGCATCCGG GTATGCTCCC AAGTCCTTCC ACGTCGAAGA CACCCCTGTC 
TGTTTCTCAA GAAACAGCTC TCTCAGTTCT CTTAGCATTG ACTCTGAGGA CGACCTGTTA 
CAGGAGTGTA TAAGTTCTGC CATGCCAAAA AAGAAAAGGC CTTCAAGACT CAAGAGTGAG 
AGCGAAAAGC AGAGCCCTAG AAAAGTGGGT GGCATATTAG CTGAAGACCT GACGCTTGAT 
TTGAAAGATC TACAGAGGCC AGATTCAGAA CACGCTTTCT CCCCCGACTC AGAAAATTTT 
GACTGGAAAG CTATTCAGGA AGGCGCAAAC TCCATAGTAA GTAGTTTGCA CCAAGCTGCT 
GCAGCCGCCG CGTGCTTATC TAGACAAGCG TCATCCGACT CAGATTCCAT TCTGTCACTA 
AAGTCCGGCA TTTCTCTGGG ATCGCCTTTT CATCTTACAC CTGATCAAGA GGAAAAGCCA 
TTCACAAGCA ATAAAGGCCC AAGAATTCTC AAACCTGGAG AGAAAAGCAC ATTAGAAGCA 
AAAAAAATAG AATCTGAAAA CAAAGGAATC AAAGGCGGGA AAAAGGTTTA TAAAAGCTTG 
ATTACGGGAA AGATTCGCTC CAATTCAGAA ATTTCCAGCC AAATGAAACA ACCCCTCCCG 
ACAAACATGC CTTCAATCTC AAGAGGCAGG ACGATGATTC ACATCCCAGG GCTTCGGAAT 
AGCTCCTCTA GTACAAGCCC TGTCTCTAAG AAAGGCCCAC CCCTCAAGAC TCCAGCCTCT 
AAAAGCCCCA GTGAAGGGCC GGGAGCTACC ACTTCTCCTC GAGGAACTAA GCCAGCAGGA 
AAGTCAGAGC TTAGCCCTAT CACCAGGCAA ACTTCCCAAA TCAGTGGGTC AAATAAGGGG 
TCTTCTAGAT CAGGATCTAG AGACTCCACT CCCTCAAGAC CTACACAGCA ACCATTAAGT 
AGGCCAATGC AGTCTCCAGG GCGAAACTCA ATTTCCCCTG GTAGAAATGG AATAAGCCCT 
CCTAACAAAC TGTCTCAGCT GCCCAGAACA TCATCTCCCA GTACTGCTTC AACTAAGTCC 
TCCGGTTCTG GGAAAATGTC ATATACATCC CCAGGTAGAC AGCTGAGCCA ACAAAATCTT 
ACCAAACAAG CAAGTTTATC CAAGAATGCC AGCAGTATCC CCAGAAGTGA GTCGGCATCT 
AAAGGACTGA ATCAGATGAG TAACGGCAAT GGGTCAAATA AAAAGGTAGA ACTTTCTAGA 
ATGTCTTCAA CTAAATCAAG TGGAAGTGAA TCAGACAGAT CAGAAAGGCC TGCATTAGTA 
CGCCAGTCTA CTTTCATCAA AGAAGCCCCA AGCCCAACCC TGAGGAGGAA ACTGGAGGAA 
TCTGCCTCAT TTGAATCCCT TTCTCCATCT TCTAGACCAG ATTCTCCCAC CAGGTCGCAG 
GCACAGACCC CAGTTTTAAG CCCTTCCCTT CCTGATATGT CTCTGTCCAC ACATCCATCT 
GTTCAGGCAG GTGGGTGGCG AAAGCTCCCG CCTAATCTCA GCCCCACTAT CGAGTATAAT 
GACGGAAGGC CCACAAAACG GCATGATATT GCACGCTCCC ATTCTGAAAG TCCTTCCAGA 
CTACCAATCA ACCGGGCGGG AACCTGGAAG CGTGAACACA GCAAACATTC CTCGTCCCTT 
CCTCGAGTGA GTACTTGGAG AAGAACTGGA AGCTCATCTT CTATTCTTTC TGCTTCATCA 
GAGTCCAGTG AAAAAGCAAA AAGTGAGGAT GAAAGGCATG TGAGCTCCAT GCCAGCACCC 
AGACAGATGA AGGAAAACCA GGTGCCCACC AAAGGAACAT GGAGGAAAAT CAAGGAAAGT 
GACATTTCTC CCACAGGCAT GGCTTCTCAG AGCGCTTCCT CAGGTGCTGC CAGTGGTGCT 
GAATCCAAGC CTCTGATCTA TCAGATGGCA CCTCCTGTCT CTAAAACAGA GGATGTTTGG 
GTGAGAATTG AGGACTGCCC CATTAACAAC CCTAGATCTG GACGGTCCCC CACAGGCAAC 
ACCCCCCCAG TGATTGACAG TGTTTCAGAG AAGGGAAGTT CAAGCATTAA AGATTCAAAA 
GACACCCATG GGAAACAGAG TGTGGGCAGT GGCAGTCCTG TGCAAACCGT GGGTCTGGAA 
ACCCGCCTCA ACTCCTTTGT TCAGGTAGAG GCCCCAGAAC AGAAAGGAAC TGAGGCAAAA 
CCAGGACAGA GTAACCCAGT CTCTATAGCA GAGACTGCTG AGACGTGTAT AGCAGAGCGT 
ACCCCTTTCA GTTCCAGTAG CTCCAGCAAG CACAGCTCAC CTAGCGGGAC TGTTGCTGCC 
AGAGTGACAC CTTTTAATTA CAACCCTAGC CCTAGGAAGA GCAGCGCAGA CAGCACTTCA 
GCCCGGCCGT CTCAGATCCC TACGCCAGTG AGCACCAACA CGAAGAAGAG AGATTCGAAG 
ACTGACAGCA CAGAATCCAG TGGAGCCCAA AGTCCTAAAC GCCATTCCGG GTCTTACCTC 
GTGACGTCTG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106153.1
CDS	101..8275
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAESPATRVDHETASVLSSSGTHSAPRRLTSHLGTKIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGRKATRGISSQELGQGLSGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250293.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250293.1

ORF Insert Sequence:

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGTCCAG CTACACGTGT GGATCACGAA ACAGCCAGTG TTTTGAGTTC TAGCGGCACG 
CACTCTGCTC CTCGAAGGTT GACAAGTCAT CTGGGGACAA AGATACGAGC TTACTGTGAA 
ACCTGTTGGG AGTGGCAGGA AGCCCACGAA CAAGGCATGG ACCAGGACAA AAACCCAATG 
CCAGCTCCTG TTGAGCATCA GATCTGTCCT GCTGTGTGTG TTCTAATGAA GCTTTCATTT 
GATGAAGAGC ATAGGCATGC AATGAATGAA CTTGGTAGGA AGGCTACCCG GGGCATTTCA 
TCCCAGGAGC TAGGTCAGGG GCTTTCAGGG GGACTGCAGG CCATTGCAGA GTTATTGCAG 
GTGGACTGTG AGATGTATGG GCTTACTAAT GACCACTACA GTGTTACTTT AAGACGGTAT 
GCTGGAATGG CTTTGACAAA CTTGACCTTT GGAGATGTTG CCAACAAGGC TACGCTGTGT 
TCTATGAAAG GCTGCATGAG AGCACTTGTG GCCCAGTTAA AATCTGAGAG TGAAGACTTA 
CAGCAGGTTA TTGCAAGTGT TTTGAGGAAT TTGTCTTGGC GAGCAGATGT AAATAGCAAA 
AAGACGTTGA GAGAAGTTGG AAGTGTGAAA GCATTGATGG AATGTGCTTT GGAAGTTAAA 
AAGGAATCAA CCCTCAAAAG CGTTTTGAGT GCCTTATGGA ACCTGTCTGC ACACTGCACT 
GAGAATAAGG CTGACATCTG TGCTGTGGAT GGAGCACTGG CATTTCTGGT TGGCACCCTC 
ACTTACCGGA GCCAGACAAA TACTTTAGCC ATTATTGAAA GTGGAGGTGG GATATTACGG 
AATGTGTCCA GCTTGATAGC TACAAACGAA GACCACAGGC AAATCCTAAG AGAGAACAAT 
TGCCTACAAA CTTTATTACA GCACTTGAAA TCTCACAGCT TGACAATAGT CAGTAATGCA 
TGTGGAACTT TGTGGAATCT CTCAGCAAGA AATCCTAAAG ACCAGGAAGC CTTGTGGGAC 
ATGGGGGCAG TGAGCATGCT CAAGAACCTC ATTCATTCCA AGCACAAAAT GATTGCCATG 
GGAAGTGCAG CAGCTTTAAG GAATCTCATG GCAAACAGAC CTGCAAAGTA TAAGGATGCC 
AATATCATGT CTCCCGGCTC AAGTCTGCCA TCCCTTCACG TTAGGAAACA GAAAGCTCTA 
GAAGCTGAGC TAGATGCTCA GCATTTATCA GAAACCTTCG ACAACATTGA CAACCTAAGT 
CCCAAGGCCT CTCACCGGAG TAAGCAGAGA CACAAGCAGA ATCTTTATGG TGACTATGCT 
TTTGACGCCA ATCGACATGA TGATAGTAGG TCAGACAATT TCAATACTGG AAACATGACT 
GTTCTTTCAC CATATTTAAA TACTACGGTA TTGCCCAGCT CTTCTTCCTC AAGGGGAAGT 
TTAGACAGTT CTCGTTCTGA GAAAGACAGA AGTTTGGAGA GAGAGCGAGG TATTGGCCTC 
AGTGCTTACC ATCCAACAAC AGAAAATGCA GGAACCTCAT CAAAACGAGG TCTGCAGATC 
ACTACCACTG CAGCCCAGAT AGCCAAAGTT ATGGAAGAAG TATCAGCCAT TCATACCTCC 
CAGGACGACA GAAGTTCTGC TTCTACCACC GAGTTCCATT GTGTGGCAGA CGACAGGAGT 
GCGGCACGAA GAAGCTCTGC CTCCCACACA CACTCAAACA CATACAACTT CACTAAGTCG 
GAAAATTCAA ATAGGACATG CTCTATGCCT TATGCCAAAG TGGAATATAA ACGATCTTCA 
AATGACAGTT TAAATAGTGT CACTAGTAGT GATGGATATG GTAAAAGAGG CCAAATGAAA 
CCCTCAGTTG AATCCTATTC TGAAGATGAT GAAAGTAAAT TTTGCAGTTA TGGTCAGTAT 
CCAGCTGACC TAGCCCATAA GATACACAGT GCAAATCATA TGGATGATAA TGATGGAGAA 
CTGGATACAC CAATAAATTA CAGTCTTAAA TATTCAGATG AGCAGTTGAA CTCAGGAAGG 
CAGAGTCCCT CACAGAATGA AAGGTGGGCA AGACCAAAGC ATGTGATAGA AGATGAAATA 
AAGCAAAACG AGCAAAGACA AGCAAGAAGC CAGAACACCA GTTATCCTGT CTATTCTGAG 
AATACCGATG ACAAACACCT CAAATTCCAA CCACATTTTG GACAACAAGA ATGTGTTTCC 
CCATATAGGT CAAGGGGAAC CAGTGGTTCA GAAACAAATC GAATGGGTTC TAGTCATGCA 
ATTAATCAAA ATGTAAACCA GTCTCTGTGT CAGGAAGATG ATTATGAAGA TGATAAACCT 
ACCAACTACA GTGAACGTTA TTCTGAGGAA GAACAACATG AAGAAGAAGA AGAGAGACCG 
ACAAATTATA GCATAAAATA TAATGAAGAG AAACATCATG TGGATCAGCC TATTGATTAT 
AGTTTAAAAT ATGCCACTGA CATTTCTTCC TCACAAAAAC CATCATTTTC ATTCTCAAAG 
AATTCATCAG CACAAAGCAC TAAACCTGAA CATCTCTCTC CAAGCAGCGA GAATACAGCT 
GTACCTCCAT CTAATGCCAA AAGGCAGAAT CAGCTGCGTC CAAGTTCAGC ACAAAGAAAT 
GGCCAGACTC AAAAAGGCAC TACTTGCAAA GTCCCCTCCA TCAACCAAGA AACAATACAG 
ACTTACTGCG TAGAAGACAC CCCAATATGT TTTTCAAGGT GCAGTTCATT ATCATCACTG 
TCATCAGCTG ACGATGAAAT AGGATGTGAT CAGACAACAC AGGAAGCAGA TTCTGCTAAT 
ACTCTGCAGA CAGCAGAAGT AAAAGAGAAT GATGTAACTC GGTCAGCTGA AGATCCTGCA 
ACTGAAGTTC CAGCAGTGTC CCAGAATGCT AGAGCCAAAC CCAGCCGACT CCAGGCTTCT 
GGCTTATCTT CAGAATCAAC CAGGCATAAT AAAGCTGTTG AGTTTTCTTC AGGAGCCAAG 
TCTCCCTCCA AAAGTGGTGC TCAGACACCC AAAAGTCCCC CAGAACACTA TGTCCAGGAG 
ACTCCGCTCG TATTCAGCAG GTGTACTTCT GTCAGCTCCC TTGACAGTTT TGAGAGTCGC 
TCCATTGCCA GCTCTGTTCA GAGTGAGCCA TGTAGTGGAA TGGTGAGTGG CATCATAAGC 
CCCAGTGACC TTCCAGATAG TCCTGGGCAG ACCATGCCAC CAAGCAGAAG CAAAACCCCT 
CCACCTCCTC CACAGACAGT GCAGGCCAAG AGAGAGGTGC CAAAAAGTAA AGTCCCTGCT 
GCTGAGAAGA GAGAGAGTGG GCCTAAGCAG ACTGCTGTAA ATGCTGCCGT GCAGAGGGTG 
CAGGTCCTTC CAGACGTGGA TACTTTGTTA CACTTCGCCA CAGAAAGTAC TCCAGACGGG 
TTTTCTTGTT CCTCCAGCCT AAGTGCTCTG AGCCTGGATG AGCCATTTAT ACAGAAAGAT 
GTAGAATTAA GAATCATGCC TCCAGTTCAG GAAAACGACA ATGGGAATGA AACTGAATCA 
GAACAGCCTG AGGAATCAAA TGAAAACCAG GATAAAGAGG TAGAAAAGCC TGACTCTGAA 
AAAGACTTAT TAGATGATTC TGATGACGAT GATATTGAAA TATTAGAAGA ATGTATTATT 
TCAGCCATGC CAACAAAGTC ATCACGCAAA GCCAAAAAAC TAGCCCAGAC TGCTTCAAAA 
TTACCTCCAC CTGTGGCAAG GAAACCAAGT CAGCTACCTG TGTATAAACT TCTGCCAGCA 
CAGAATAGGC TGCAGGCACA AAAACATGTT AGCTTTACAC CAGGGGATGA TGTGCCCCGG 
GTGTACTGTG TAGAAGGGAC ACCTATAAAC TTTTCCACAG CAACGTCTCT AAGTGATCTG 
ACAATAGAGT CCCCTCCAAA TGAATTGGCT ACTGGAGATG GGGTCAGAGC GGGTATACAG 
TCAGGTGAAT TTGAAAAACG AGATACCATT CCTACAGAAG GCAGAAGTAC AGATGATGCT 
CAGCGAGGAA AAATCTCATC TATAGTTACA CCAGACCTGG ATGACAACAA AGCAGAGGAA 
GGAGATATTC TTGCAGAATG TATCAATTCT GCTATGCCCA AAGGAAAAAG CCACAAGCCT 
TTCCGAGTGA AAAAGATAAT GGACCAAGTC CAACAAGCAT CCTCGACTTC ATCTGGAGCT 
AACAAAAATC AAGTAGACAC TAAGAAAAAG AAGCCTACTT CACCAGTAAA GCCCATGCCA 
CAAAATACTG AATATAGAAC GCGTGTGAGA AAGAATACAG ACTCAAAAGT TAATGTAAAT 
ACTGAAGAAA CTTTCTCAGA CAACAAAGAC TCAAAGAAAC CAAGCTTACA AACCAATGCC 
AAGGCCTTCA ATGAAAAGCT ACCTAACAAT GAAGACAGAG TGCGGGGGAG CTTCGCCTTG 
GACTCACCGC ATCACTACAC CCCTATTGAG GGGACGCCGT ACTGCTTTTC CCGAAATGAC 
TCCTTGAGTT CTCTGGATTT TGATGATGAC GATGTTGACC TTTCCAGGGA AAAGGCCGAG 
TTAAGAAAGG GCAAAGAAAG CAAGGATTCC GAAGCCAAAG TTACCTGCCG CCCAGAACCA 
AACTCAAGCC AGCAGGCAGC TAGTAAGTCA CAAGCCAGTA TAAAACATCC AGCAAACAGA 
GCACAGTCCA AACCAGTGCT GCAGAAACAG CCCACTTTCC CCCAGTCCTC CAAAGACGGA 
CCAGATAGAG GGGCAGCAAC TGACGAAAAA CTGCAGAATT TTGCTATTGA AAATACTCCA 
GTTTGCTTTT CTCGAAATTC CTCTCTGAGT TCCCTTAGTG ACATTGACCA GGAAAACAAC 
AATAACAAAG AAAGTGAACC AATCAAAGAA GCTGAACCTG CCAACTCACA AGGAGAGCCC 
AGTAAGCCTC AGGCATCCGG GTATGCTCCC AAGTCCTTCC ACGTCGAAGA CACCCCTGTC 
TGTTTCTCAA GAAACAGCTC TCTCAGTTCT CTTAGCATTG ACTCTGAGGA CGACCTGTTA 
CAGGAGTGTA TAAGTTCTGC CATGCCAAAA AAGAAAAGGC CTTCAAGACT CAAGAGTGAG 
AGCGAAAAGC AGAGCCCTAG AAAAGTGGGT GGCATATTAG CTGAAGACCT GACGCTTGAT 
TTGAAAGATC TACAGAGGCC AGATTCAGAA CACGCTTTCT CCCCCGACTC AGAAAATTTT 
GACTGGAAAG CTATTCAGGA AGGCGCAAAC TCCATAGTAA GTAGTTTGCA CCAAGCTGCT 
GCAGCCGCCG CGTGCTTATC TAGACAAGCG TCATCCGACT CAGATTCCAT TCTGTCACTA 
AAGTCCGGCA TTTCTCTGGG ATCGCCTTTT CATCTTACAC CTGATCAAGA GGAAAAGCCA 
TTCACAAGCA ATAAAGGCCC AAGAATTCTC AAACCTGGAG AGAAAAGCAC ATTAGAAGCA 
AAAAAAATAG AATCTGAAAA CAAAGGAATC AAAGGCGGGA AAAAGGTTTA TAAAAGCTTG 
ATTACGGGAA AGATTCGCTC CAATTCAGAA ATTTCCAGCC AAATGAAACA ACCCCTCCCG 
ACAAACATGC CTTCAATCTC AAGAGGCAGG ACGATGATTC ACATCCCAGG GCTTCGGAAT 
AGCTCCTCTA GTACAAGCCC TGTCTCTAAG AAAGGCCCAC CCCTCAAGAC TCCAGCCTCT 
AAAAGCCCCA GTGAAGGGCC GGGAGCTACC ACTTCTCCTC GAGGAACTAA GCCAGCAGGA 
AAGTCAGAGC TTAGCCCTAT CACCAGGCAA ACTTCCCAAA TCAGTGGGTC AAATAAGGGG 
TCTTCTAGAT CAGGATCTAG AGACTCCACT CCCTCAAGAC CTACACAGCA ACCATTAAGT 
AGGCCAATGC AGTCTCCAGG GCGAAACTCA ATTTCCCCTG GTAGAAATGG AATAAGCCCT 
CCTAACAAAC TGTCTCAGCT GCCCAGAACA TCATCTCCCA GTACTGCTTC AACTAAGTCC 
TCCGGTTCTG GGAAAATGTC ATATACATCC CCAGGTAGAC AGCTGAGCCA ACAAAATCTT 
ACCAAACAAG CAAGTTTATC CAAGAATGCC AGCAGTATCC CCAGAAGTGA GTCGGCATCT 
AAAGGACTGA ATCAGATGAG TAACGGCAAT GGGTCAAATA AAAAGGTAGA ACTTTCTAGA 
ATGTCTTCAA CTAAATCAAG TGGAAGTGAA TCAGACAGAT CAGAAAGGCC TGCATTAGTA 
CGCCAGTCTA CTTTCATCAA AGAAGCCCCA AGCCCAACCC TGAGGAGGAA ACTGGAGGAA 
TCTGCCTCAT TTGAATCCCT TTCTCCATCT TCTAGACCAG ATTCTCCCAC CAGGTCGCAG 
GCACAGACCC CAGTTTTAAG CCCTTCCCTT CCTGATATGT CTCTGTCCAC ACATCCATCT 
GTTCAGGCAG GTGGGTGGCG AAAGCTCCCG CCTAATCTCA GCCCCACTAT CGAGTATAAT 
GACGGAAGGC CCACAAAACG GCATGATATT GCACGCTCCC ATTCTGAAAG TCCTTCCAGA 
CTACCAATCA ACCGGGCGGG AACCTGGAAG CGTGAACACA GCAAACATTC CTCGTCCCTT 
CCTCGAGTGA GTACTTGGAG AAGAACTGGA AGCTCATCTT CTATTCTTTC TGCTTCATCA 
GAGTCCAGTG AAAAAGCAAA AAGTGAGGAT GAAAGGCATG TGAGCTCCAT GCCAGCACCC 
AGACAGATGA AGGAAAACCA GGTGCCCACC AAAGGAACAT GGAGGAAAAT CAAGGAAAGT 
GACATTTCTC CCACAGGCAT GGCTTCTCAG AGCGCTTCCT CAGGTGCTGC CAGTGGTGCT 
GAATCCAAGC CTCTGATCTA TCAGATGGCA CCTCCTGTCT CTAAAACAGA GGATGTTTGG 
GTGAGAATTG AGGACTGCCC CATTAACAAC CCTAGATCTG GACGGTCCCC CACAGGCAAC 
ACCCCCCCAG TGATTGACAG TGTTTCAGAG AAGGGAAGTT CAAGCATTAA AGATTCAAAA 
GACACCCATG GGAAACAGAG TGTGGGCAGT GGCAGTCCTG TGCAAACCGT GGGTCTGGAA 
ACCCGCCTCA ACTCCTTTGT TCAGGTAGAG GCCCCAGAAC AGAAAGGAAC TGAGGCAAAA 
CCAGGACAGA GTAACCCAGT CTCTATAGCA GAGACTGCTG AGACGTGTAT AGCAGAGCGT 
ACCCCTTTCA GTTCCAGTAG CTCCAGCAAG CACAGCTCAC CTAGCGGGAC TGTTGCTGCC 
AGAGTGACAC CTTTTAATTA CAACCCTAGC CCTAGGAAGA GCAGCGCAGA CAGCACTTCA 
GCCCGGCCGT CTCAGATCCC TACGCCAGTG AGCACCAACA CGAAGAAGAG AGATTCGAAG 
ACTGACAGCA CAGAATCCAG TGGAGCCCAA AGTCCTAAAC GCCATTCCGG GTCTTACCTC 
GTGACGTCTG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250294.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106154.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X26, mRNA.

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CloneID	OMu87332
Clone ID Related Accession (Same CDS sequence)	XM_030250294.1
Accession Version	XM_030250294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGTCCAG CTACACGTGT GGATCACGAA ACAGCCAGTG TTTTGAGTTC TAGCGGCACG 
CACTCTGCTC CTCGAAGGTT GACAAGTCAT CTGGGGACAA AGATACGAGC TTACTGTGAA 
ACCTGTTGGG AGTGGCAGGA AGCCCACGAA CAAGGCATGG ACCAGGACAA AAACCCAATG 
CCAGCTCCTG TTGAGCATCA GATCTGTCCT GCTGTGTGTG TTCTAATGAA GCTTTCATTT 
GATGAAGAGC ATAGGCATGC AATGAATGAA CTTGGGGGAC TGCAGGCCAT TGCAGAGTTA 
TTGCAGGTGG ACTGTGAGAT GTATGGGCTT ACTAATGACC ACTACAGTGT TACTTTAAGA 
CGGTATGCTG GAATGGCTTT GACAAACTTG ACCTTTGGAG ATGTTGCCAA CAAGGCTACG 
CTGTGTTCTA TGAAAGGCTG CATGAGAGCA CTTGTGGCCC AGTTAAAATC TGAGAGTGAA 
GACTTACAGC AGGTTATTGC AAGTGTTTTG AGGAATTTGT CTTGGCGAGC AGATGTAAAT 
AGCAAAAAGA CGTTGAGAGA AGTTGGAAGT GTGAAAGCAT TGATGGAATG TGCTTTGGAA 
GTTAAAAAGG AATCAACCCT CAAAAGCGTT TTGAGTGCCT TATGGAACCT GTCTGCACAC 
TGCACTGAGA ATAAGGCTGA CATCTGTGCT GTGGATGGAG CACTGGCATT TCTGGTTGGC 
ACCCTCACTT ACCGGAGCCA GACAAATACT TTAGCCATTA TTGAAAGTGG AGGTGGGATA 
TTACGGAATG TGTCCAGCTT GATAGCTACA AACGAAGACC ACAGGCAAAT CCTAAGAGAG 
AACAATTGCC TACAAACTTT ATTACAGCAC TTGAAATCTC ACAGCTTGAC AATAGTCAGT 
AATGCATGTG GAACTTTGTG GAATCTCTCA GCAAGAAATC CTAAAGACCA GGAAGCCTTG 
TGGGACATGG GGGCAGTGAG CATGCTCAAG AACCTCATTC ATTCCAAGCA CAAAATGATT 
GCCATGGGAA GTGCAGCAGC TTTAAGGAAT CTCATGGCAA ACAGACCTGC AAAGTATAAG 
GATGCCAATA TCATGTCTCC CGGCTCAAGT CTGCCATCCC TTCACGTTAG GAAACAGAAA 
GCTCTAGAAG CTGAGCTAGA TGCTCAGCAT TTATCAGAAA CCTTCGACAA CATTGACAAC 
CTAAGTCCCA AGGCCTCTCA CCGGAGTAAG CAGAGACACA AGCAGAATCT TTATGGTGAC 
TATGCTTTTG ACGCCAATCG ACATGATGAT AGTAGGTCAG ACAATTTCAA TACTGGAAAC 
ATGACTGTTC TTTCACCATA TTTAAATACT ACGGTATTGC CCAGCTCTTC TTCCTCAAGG 
GGAAGTTTAG ACAGTTCTCG TTCTGAGAAA GACAGAAGTT TGGAGAGAGA GCGAGGTATT 
GGCCTCAGTG CTTACCATCC AACAACAGAA AATGCAGGAA CCTCATCAAA ACGAGGTCTG 
CAGATCACTA CCACTGCAGC CCAGATAGCC AAAGTTATGG AAGAAGTATC AGCCATTCAT 
ACCTCCCAGG ACGACAGAAG TTCTGCTTCT ACCACCGAGT TCCATTGTGT GGCAGACGAC 
AGGAGTGCGG CACGAAGAAG CTCTGCCTCC CACACACACT CAAACACATA CAACTTCACT 
AAGTCGGAAA ATTCAAATAG GACATGCTCT ATGCCTTATG CCAAAGTGGA ATATAAACGA 
TCTTCAAATG ACAGTTTAAA TAGTGTCACT AGTAGTGATG GATATGGTAA AAGAGGCCAA 
ATGAAACCCT CAGTTGAATC CTATTCTGAA GATGATGAAA GTAAATTTTG CAGTTATGGT 
CAGTATCCAG CTGACCTAGC CCATAAGATA CACAGTGCAA ATCATATGGA TGATAATGAT 
GGAGAACTGG ATACACCAAT AAATTACAGT CTTAAATATT CAGATGAGCA GTTGAACTCA 
GGAAGGCAGA GTCCCTCACA GAATGAAAGG TGGGCAAGAC CAAAGCATGT GATAGAAGAT 
GAAATAAAGC AAAACGAGCA AAGACAAGCA AGAAGCCAGA ACACCAGTTA TCCTGTCTAT 
TCTGAGAATA CCGATGACAA ACACCTCAAA TTCCAACCAC ATTTTGGACA ACAAGAATGT 
GTTTCCCCAT ATAGGTCAAG GGGAACCAGT GGTTCAGAAA CAAATCGAAT GGGTTCTAGT 
CATGCAATTA ATCAAAATGT AAACCAGTCT CTGTGTCAGG AAGATGATTA TGAAGATGAT 
AAACCTACCA ACTACAGTGA ACGTTATTCT GAGGAAGAAC AACATGAAGA AGAAGAAGAG 
AGACCGACAA ATTATAGCAT AAAATATAAT GAAGAGAAAC ATCATGTGGA TCAGCCTATT 
GATTATAGTT TAAAATATGC CACTGACATT TCTTCCTCAC AAAAACCATC ATTTTCATTC 
TCAAAGAATT CATCAGCACA AAGCACTAAA CCTGAACATC TCTCTCCAAG CAGCGAGAAT 
ACAGCTGTAC CTCCATCTAA TGCCAAAAGG CAGAATCAGC TGCGTCCAAG TTCAGCACAA 
AGAAATGGCC AGACTCAAAA AGGCACTACT TGCAAAGTCC CCTCCATCAA CCAAGAAACA 
ATACAGACTT ACTGCGTAGA AGACACCCCA ATATGTTTTT CAAGGTGCAG TTCATTATCA 
TCACTGTCAT CAGCTGACGA TGAAATAGGA TGTGATCAGA CAACACAGGA AGCAGATTCT 
GCTAATACTC TGCAGACAGC AGAAGTAAAA GAGAATGATG TAACTCGGTC AGCTGAAGAT 
CCTGCAACTG AAGTTCCAGC AGTGTCCCAG AATGCTAGAG CCAAACCCAG CCGACTCCAG 
GCTTCTGGCT TATCTTCAGA ATCAACCAGG CATAATAAAG CTGTTGAGTT TTCTTCAGGA 
GCCAAGTCTC CCTCCAAAAG TGGTGCTCAG ACACCCAAAA GTCCCCCAGA ACACTATGTC 
CAGGAGACTC CGCTCGTATT CAGCAGGTGT ACTTCTGTCA GCTCCCTTGA CAGTTTTGAG 
AGTCGCTCCA TTGCCAGCTC TGTTCAGAGT GAGCCATGTA GTGGAATGGT GAGTGGCATC 
ATAAGCCCCA GTGACCTTCC AGATAGTCCT GGGCAGACCA TGCCACCAAG CAGAAGCAAA 
ACCCCTCCAC CTCCTCCACA GACAGTGCAG GCCAAGAGAG AGGTGCCAAA AAGTAAAGTC 
CCTGCTGCTG AGAAGAGAGA GAGTGGGCCT AAGCAGACTG CTGTAAATGC TGCCGTGCAG 
AGGGTGCAGG TCCTTCCAGA CGTGGATACT TTGTTACACT TCGCCACAGA AAGTACTCCA 
GACGGGTTTT CTTGTTCCTC CAGCCTAAGT GCTCTGAGCC TGGATGAGCC ATTTATACAG 
AAAGATGTAG AATTAAGAAT CATGCCTCCA GTTCAGGAAA ACGACAATGG GAATGAAACT 
GAATCAGAAC AGCCTGAGGA ATCAAATGAA AACCAGGATA AAGAGGTAGA AAAGCCTGAC 
TCTGAAAAAG ACTTATTAGA TGATTCTGAT GACGATGATA TTGAAATATT AGAAGAATGT 
ATTATTTCAG CCATGCCAAC AAAGTCATCA CGCAAAGCCA AAAAACTAGC CCAGACTGCT 
TCAAAATTAC CTCCACCTGT GGCAAGGAAA CCAAGTCAGC TACCTGTGTA TAAACTTCTG 
CCAGCACAGA ATAGGCTGCA GGCACAAAAA CATGTTAGCT TTACACCAGG GGATGATGTG 
CCCCGGGTGT ACTGTGTAGA AGGGACACCT ATAAACTTTT CCACAGCAAC GTCTCTAAGT 
GATCTGACAA TAGAGTCCCC TCCAAATGAA TTGGCTACTG GAGATGGGGT CAGAGCGGGT 
ATACAGTCAG GTGAATTTGA AAAACGAGAT ACCATTCCTA CAGAAGGCAG AAGTACAGAT 
GATGCTCAGC GAGGAAAAAT CTCATCTATA GTTACACCAG ACCTGGATGA CAACAAAGCA 
GAGGAAGGAG ATATTCTTGC AGAATGTATC AATTCTGCTA TGCCCAAAGG AAAAAGCCAC 
AAGCCTTTCC GAGTGAAAAA GATAATGGAC CAAGTCCAAC AAGCATCCTC GACTTCATCT 
GGAGCTAACA AAAATCAAGT AGACACTAAG AAAAAGAAGC CTACTTCACC AGTAAAGCCC 
ATGCCACAAA ATACTGAATA TAGAACGCGT GTGAGAAAGA ATACAGACTC AAAAGTTAAT 
GTAAATACTG AAGAAACTTT CTCAGACAAC AAAGACTCAA AGAAACCAAG CTTACAAACC 
AATGCCAAGG CCTTCAATGA AAAGCTACCT AACAATGAAG ACAGAGTGCG GGGGAGCTTC 
GCCTTGGACT CACCGCATCA CTACACCCCT ATTGAGGGGA CGCCGTACTG CTTTTCCCGA 
AATGACTCCT TGAGTTCTCT GGATTTTGAT GATGACGATG TTGACCTTTC CAGGGAAAAG 
GCCGAGTTAA GAAAGGGCAA AGAAAGCAAG GATTCCGAAG CCAAAGTTAC CTGCCGCCCA 
GAACCAAACT CAAGCCAGCA GGCAGCTAGT AAGTCACAAG CCAGTATAAA ACATCCAGCA 
AACAGAGCAC AGTCCAAACC AGTGCTGCAG AAACAGCCCA CTTTCCCCCA GTCCTCCAAA 
GACGGACCAG ATAGAGGGGC AGCAACTGAC GAAAAACTGC AGAATTTTGC TATTGAAAAT 
ACTCCAGTTT GCTTTTCTCG AAATTCCTCT CTGAGTTCCC TTAGTGACAT TGACCAGGAA 
AACAACAATA ACAAAGAAAG TGAACCAATC AAAGAAGCTG AACCTGCCAA CTCACAAGGA 
GAGCCCAGTA AGCCTCAGGC ATCCGGGTAT GCTCCCAAGT CCTTCCACGT CGAAGACACC 
CCTGTCTGTT TCTCAAGAAA CAGCTCTCTC AGTTCTCTTA GCATTGACTC TGAGGACGAC 
CTGTTACAGG AGTGTATAAG TTCTGCCATG CCAAAAAAGA AAAGGCCTTC AAGACTCAAG 
AGTGAGAGCG AAAAGCAGAG CCCTAGAAAA GTGGGTGGCA TATTAGCTGA AGACCTGACG 
CTTGATTTGA AAGATCTACA GAGGCCAGAT TCAGAACACG CTTTCTCCCC CGACTCAGAA 
AATTTTGACT GGAAAGCTAT TCAGGAAGGC GCAAACTCCA TAGTAAGTAG TTTGCACCAA 
GCTGCTGCAG CCGCCGCGTG CTTATCTAGA CAAGCGTCAT CCGACTCAGA TTCCATTCTG 
TCACTAAAGT CCGGCATTTC TCTGGGATCG CCTTTTCATC TTACACCTGA TCAAGAGGAA 
AAGCCATTCA CAAGCAATAA AGGCCCAAGA ATTCTCAAAC CTGGAGAGAA AAGCACATTA 
GAAGCAAAAA AAATAGAATC TGAAAACAAA GGAATCAAAG GCGGGAAAAA GGTTTATAAA 
AGCTTGATTA CGGGAAAGAT TCGCTCCAAT TCAGAAATTT CCAGCCAAAT GAAACAACCC 
CTCCCGACAA ACATGCCTTC AATCTCAAGA GGCAGGACGA TGATTCACAT CCCAGGGCTT 
CGGAATAGCT CCTCTAGTAC AAGCCCTGTC TCTAAGAAAG GCCCACCCCT CAAGACTCCA 
GCCTCTAAAA GCCCCAGTGA AGGGCCGGGA GCTACCACTT CTCCTCGAGG AACTAAGCCA 
GCAGGAAAGT CAGAGCTTAG CCCTATCACC AGGCAAACTT CCCAAATCAG TGGGTCAAAT 
AAGGGGTCTT CTAGATCAGG ATCTAGAGAC TCCACTCCCT CAAGACCTAC ACAGCAACCA 
TTAAGTAGGC CAATGCAGTC TCCAGGGCGA AACTCAATTT CCCCTGGTAG AAATGGAATA 
AGCCCTCCTA ACAAACTGTC TCAGCTGCCC AGAACATCAT CTCCCAGTAC TGCTTCAACT 
AAGTCCTCCG GTTCTGGGAA AATGTCATAT ACATCCCCAG GTAGACAGCT GAGCCAACAA 
AATCTTACCA AACAAGCAAG TTTATCCAAG AATGCCAGCA GTATCCCCAG AAGTGAGTCG 
GCATCTAAAG GACTGAATCA GATGAGTAAC GGCAATGGGT CAAATAAAAA GGTAGAACTT 
TCTAGAATGT CTTCAACTAA ATCAAGTGGA AGTGAATCAG ACAGATCAGA AAGGCCTGCA 
TTAGTACGCC AGTCTACTTT CATCAAAGAA GCCCCAAGCC CAACCCTGAG GAGGAAACTG 
GAGGAATCTG CCTCATTTGA ATCCCTTTCT CCATCTTCTA GACCAGATTC TCCCACCAGG 
TCGCAGGCAC AGACCCCAGT TTTAAGCCCT TCCCTTCCTG ATATGTCTCT GTCCACACAT 
CCATCTGTTC AGGCAGGTGG GTGGCGAAAG CTCCCGCCTA ATCTCAGCCC CACTATCGAG 
TATAATGACG GAAGGCCCAC AAAACGGCAT GATATTGCAC GCTCCCATTC TGAAAGTCCT 
TCCAGACTAC CAATCAACCG GGCGGGAACC TGGAAGCGTG AACACAGCAA ACATTCCTCG 
TCCCTTCCTC GAGTGAGTAC TTGGAGAAGA ACTGGAAGCT CATCTTCTAT TCTTTCTGCT 
TCATCAGAGT CCAGTGAAAA AGCAAAAAGT GAGGATGAAA GGCATGTGAG CTCCATGCCA 
GCACCCAGAC AGATGAAGGA AAACCAGGTG CCCACCAAAG GAACATGGAG GAAAATCAAG 
GAAAGTGACA TTTCTCCCAC AGGCATGGCT TCTCAGAGCG CTTCCTCAGG TGCTGCCAGT 
GGTGCTGAAT CCAAGCCTCT GATCTATCAG ATGGCACCTC CTGTCTCTAA AACAGAGGAT 
GTTTGGGTGA GAATTGAGGA CTGCCCCATT AACAACCCTA GATCTGGACG GTCCCCCACA 
GGCAACACCC CCCCAGTGAT TGACAGTGTT TCAGAGAAGG GAAGTTCAAG CATTAAAGAT 
TCAAAAGACA CCCATGGGAA ACAGAGTGTG GGCAGTGGCA GTCCTGTGCA AACCGTGGGT 
CTGGAAACCC GCCTCAACTC CTTTGTTCAG GTAGAGGCCC CAGAACAGAA AGGAACTGAG 
GCAAAACCAG GACAGAGTAA CCCAGTCTCT ATAGCAGAGA CTGCTGAGAC GTGTATAGCA 
GAGCGTACCC CTTTCAGTTC CAGTAGCTCC AGCAAGCACA GCTCACCTAG CGGGACTGTT 
GCTGCCAGAG TGACACCTTT TAATTACAAC CCTAGCCCTA GGAAGAGCAG CGCAGACAGC 
ACTTCAGCCC GGCCGTCTCA GATCCCTACG CCAGTGAGCA CCAACACGAA GAAGAGAGAT 
TCGAAGACTG ACAGCACAGA ATCCAGTGGA GCCCAAAGTC CTAAACGCCA TTCCGGGTCT 
TACCTCGTGA CGTCTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106154.1
CDS	101..8221
Translation	MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLTKLETEASNMKEVLKQLQGSIEDETMTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAESPATRVDHETASVLSSSGTHSAPRRLTSHLGTKIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250294.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250294.1

ORF Insert Sequence:

ATGGCTGCAG CTTCATATGA TCAGTTGTTA AAGCAAGTTG AGGCACTGAA GATGGAGAAC 
TCAAATCTTC GACAAGAGCT AGAAGATAAT TCCAATCATC TTACAAAACT GGAAACTGAG 
GCATCTAATA TGAAGGAAGT ACTTAAGCAG CTACAGGGAA GTATTGAAGA TGAGACTATG 
ACTTCTGGAC AGATTGACTT ACTAGAGCGT CTTAAAGAAT TTAACTTAGA TAGTAATTTC 
CCCGGAGTGA AACTACGCTC AAAAATGTCC CTTCGCTCCT ACGGAAGTCG GGAAGGATCT 
GTATCCAGCC GTTCAGGAGA ATGCAGTCCT GTCCCCATGG GGTCATTCCC AAGAAGAACA 
TTTGTAAATG GAAGCAGAGA GAGTACTGGG TATCTAGAAG AGCTTGAAAA AGAAAGATCA 
TTACTCCTTG CTGATCTTGA CAAAGAAGAG AAGGAAAAGG ACTGGTATTA TGCTCAACTT 
CAGAACCTCA CAAAAAGAAT AGATAGCCTG CCTTTAACTG AAAATTTTTC CTTACAGACA 
GACATGACAA GACGGCAGCT GGAGTATGAA GCAAGGCAGA TCAGGGCTGC AATGGAGGAG 
CAGCTTGGCA CCTGCCAGGA CATGGAGAAG CGTGCACAGC GAAGAATAGC CAGGATCCAG 
CAAATAGAAA AGGACATACT GCGCGTGCGC CAGCTTTTAC AGTCCCAGGC GGCGGAAGCG 
GAGAGTCCAG CTACACGTGT GGATCACGAA ACAGCCAGTG TTTTGAGTTC TAGCGGCACG 
CACTCTGCTC CTCGAAGGTT GACAAGTCAT CTGGGGACAA AGATACGAGC TTACTGTGAA 
ACCTGTTGGG AGTGGCAGGA AGCCCACGAA CAAGGCATGG ACCAGGACAA AAACCCAATG 
CCAGCTCCTG TTGAGCATCA GATCTGTCCT GCTGTGTGTG TTCTAATGAA GCTTTCATTT 
GATGAAGAGC ATAGGCATGC AATGAATGAA CTTGGGGGAC TGCAGGCCAT TGCAGAGTTA 
TTGCAGGTGG ACTGTGAGAT GTATGGGCTT ACTAATGACC ACTACAGTGT TACTTTAAGA 
CGGTATGCTG GAATGGCTTT GACAAACTTG ACCTTTGGAG ATGTTGCCAA CAAGGCTACG 
CTGTGTTCTA TGAAAGGCTG CATGAGAGCA CTTGTGGCCC AGTTAAAATC TGAGAGTGAA 
GACTTACAGC AGGTTATTGC AAGTGTTTTG AGGAATTTGT CTTGGCGAGC AGATGTAAAT 
AGCAAAAAGA CGTTGAGAGA AGTTGGAAGT GTGAAAGCAT TGATGGAATG TGCTTTGGAA 
GTTAAAAAGG AATCAACCCT CAAAAGCGTT TTGAGTGCCT TATGGAACCT GTCTGCACAC 
TGCACTGAGA ATAAGGCTGA CATCTGTGCT GTGGATGGAG CACTGGCATT TCTGGTTGGC 
ACCCTCACTT ACCGGAGCCA GACAAATACT TTAGCCATTA TTGAAAGTGG AGGTGGGATA 
TTACGGAATG TGTCCAGCTT GATAGCTACA AACGAAGACC ACAGGCAAAT CCTAAGAGAG 
AACAATTGCC TACAAACTTT ATTACAGCAC TTGAAATCTC ACAGCTTGAC AATAGTCAGT 
AATGCATGTG GAACTTTGTG GAATCTCTCA GCAAGAAATC CTAAAGACCA GGAAGCCTTG 
TGGGACATGG GGGCAGTGAG CATGCTCAAG AACCTCATTC ATTCCAAGCA CAAAATGATT 
GCCATGGGAA GTGCAGCAGC TTTAAGGAAT CTCATGGCAA ACAGACCTGC AAAGTATAAG 
GATGCCAATA TCATGTCTCC CGGCTCAAGT CTGCCATCCC TTCACGTTAG GAAACAGAAA 
GCTCTAGAAG CTGAGCTAGA TGCTCAGCAT TTATCAGAAA CCTTCGACAA CATTGACAAC 
CTAAGTCCCA AGGCCTCTCA CCGGAGTAAG CAGAGACACA AGCAGAATCT TTATGGTGAC 
TATGCTTTTG ACGCCAATCG ACATGATGAT AGTAGGTCAG ACAATTTCAA TACTGGAAAC 
ATGACTGTTC TTTCACCATA TTTAAATACT ACGGTATTGC CCAGCTCTTC TTCCTCAAGG 
GGAAGTTTAG ACAGTTCTCG TTCTGAGAAA GACAGAAGTT TGGAGAGAGA GCGAGGTATT 
GGCCTCAGTG CTTACCATCC AACAACAGAA AATGCAGGAA CCTCATCAAA ACGAGGTCTG 
CAGATCACTA CCACTGCAGC CCAGATAGCC AAAGTTATGG AAGAAGTATC AGCCATTCAT 
ACCTCCCAGG ACGACAGAAG TTCTGCTTCT ACCACCGAGT TCCATTGTGT GGCAGACGAC 
AGGAGTGCGG CACGAAGAAG CTCTGCCTCC CACACACACT CAAACACATA CAACTTCACT 
AAGTCGGAAA ATTCAAATAG GACATGCTCT ATGCCTTATG CCAAAGTGGA ATATAAACGA 
TCTTCAAATG ACAGTTTAAA TAGTGTCACT AGTAGTGATG GATATGGTAA AAGAGGCCAA 
ATGAAACCCT CAGTTGAATC CTATTCTGAA GATGATGAAA GTAAATTTTG CAGTTATGGT 
CAGTATCCAG CTGACCTAGC CCATAAGATA CACAGTGCAA ATCATATGGA TGATAATGAT 
GGAGAACTGG ATACACCAAT AAATTACAGT CTTAAATATT CAGATGAGCA GTTGAACTCA 
GGAAGGCAGA GTCCCTCACA GAATGAAAGG TGGGCAAGAC CAAAGCATGT GATAGAAGAT 
GAAATAAAGC AAAACGAGCA AAGACAAGCA AGAAGCCAGA ACACCAGTTA TCCTGTCTAT 
TCTGAGAATA CCGATGACAA ACACCTCAAA TTCCAACCAC ATTTTGGACA ACAAGAATGT 
GTTTCCCCAT ATAGGTCAAG GGGAACCAGT GGTTCAGAAA CAAATCGAAT GGGTTCTAGT 
CATGCAATTA ATCAAAATGT AAACCAGTCT CTGTGTCAGG AAGATGATTA TGAAGATGAT 
AAACCTACCA ACTACAGTGA ACGTTATTCT GAGGAAGAAC AACATGAAGA AGAAGAAGAG 
AGACCGACAA ATTATAGCAT AAAATATAAT GAAGAGAAAC ATCATGTGGA TCAGCCTATT 
GATTATAGTT TAAAATATGC CACTGACATT TCTTCCTCAC AAAAACCATC ATTTTCATTC 
TCAAAGAATT CATCAGCACA AAGCACTAAA CCTGAACATC TCTCTCCAAG CAGCGAGAAT 
ACAGCTGTAC CTCCATCTAA TGCCAAAAGG CAGAATCAGC TGCGTCCAAG TTCAGCACAA 
AGAAATGGCC AGACTCAAAA AGGCACTACT TGCAAAGTCC CCTCCATCAA CCAAGAAACA 
ATACAGACTT ACTGCGTAGA AGACACCCCA ATATGTTTTT CAAGGTGCAG TTCATTATCA 
TCACTGTCAT CAGCTGACGA TGAAATAGGA TGTGATCAGA CAACACAGGA AGCAGATTCT 
GCTAATACTC TGCAGACAGC AGAAGTAAAA GAGAATGATG TAACTCGGTC AGCTGAAGAT 
CCTGCAACTG AAGTTCCAGC AGTGTCCCAG AATGCTAGAG CCAAACCCAG CCGACTCCAG 
GCTTCTGGCT TATCTTCAGA ATCAACCAGG CATAATAAAG CTGTTGAGTT TTCTTCAGGA 
GCCAAGTCTC CCTCCAAAAG TGGTGCTCAG ACACCCAAAA GTCCCCCAGA ACACTATGTC 
CAGGAGACTC CGCTCGTATT CAGCAGGTGT ACTTCTGTCA GCTCCCTTGA CAGTTTTGAG 
AGTCGCTCCA TTGCCAGCTC TGTTCAGAGT GAGCCATGTA GTGGAATGGT GAGTGGCATC 
ATAAGCCCCA GTGACCTTCC AGATAGTCCT GGGCAGACCA TGCCACCAAG CAGAAGCAAA 
ACCCCTCCAC CTCCTCCACA GACAGTGCAG GCCAAGAGAG AGGTGCCAAA AAGTAAAGTC 
CCTGCTGCTG AGAAGAGAGA GAGTGGGCCT AAGCAGACTG CTGTAAATGC TGCCGTGCAG 
AGGGTGCAGG TCCTTCCAGA CGTGGATACT TTGTTACACT TCGCCACAGA AAGTACTCCA 
GACGGGTTTT CTTGTTCCTC CAGCCTAAGT GCTCTGAGCC TGGATGAGCC ATTTATACAG 
AAAGATGTAG AATTAAGAAT CATGCCTCCA GTTCAGGAAA ACGACAATGG GAATGAAACT 
GAATCAGAAC AGCCTGAGGA ATCAAATGAA AACCAGGATA AAGAGGTAGA AAAGCCTGAC 
TCTGAAAAAG ACTTATTAGA TGATTCTGAT GACGATGATA TTGAAATATT AGAAGAATGT 
ATTATTTCAG CCATGCCAAC AAAGTCATCA CGCAAAGCCA AAAAACTAGC CCAGACTGCT 
TCAAAATTAC CTCCACCTGT GGCAAGGAAA CCAAGTCAGC TACCTGTGTA TAAACTTCTG 
CCAGCACAGA ATAGGCTGCA GGCACAAAAA CATGTTAGCT TTACACCAGG GGATGATGTG 
CCCCGGGTGT ACTGTGTAGA AGGGACACCT ATAAACTTTT CCACAGCAAC GTCTCTAAGT 
GATCTGACAA TAGAGTCCCC TCCAAATGAA TTGGCTACTG GAGATGGGGT CAGAGCGGGT 
ATACAGTCAG GTGAATTTGA AAAACGAGAT ACCATTCCTA CAGAAGGCAG AAGTACAGAT 
GATGCTCAGC GAGGAAAAAT CTCATCTATA GTTACACCAG ACCTGGATGA CAACAAAGCA 
GAGGAAGGAG ATATTCTTGC AGAATGTATC AATTCTGCTA TGCCCAAAGG AAAAAGCCAC 
AAGCCTTTCC GAGTGAAAAA GATAATGGAC CAAGTCCAAC AAGCATCCTC GACTTCATCT 
GGAGCTAACA AAAATCAAGT AGACACTAAG AAAAAGAAGC CTACTTCACC AGTAAAGCCC 
ATGCCACAAA ATACTGAATA TAGAACGCGT GTGAGAAAGA ATACAGACTC AAAAGTTAAT 
GTAAATACTG AAGAAACTTT CTCAGACAAC AAAGACTCAA AGAAACCAAG CTTACAAACC 
AATGCCAAGG CCTTCAATGA AAAGCTACCT AACAATGAAG ACAGAGTGCG GGGGAGCTTC 
GCCTTGGACT CACCGCATCA CTACACCCCT ATTGAGGGGA CGCCGTACTG CTTTTCCCGA 
AATGACTCCT TGAGTTCTCT GGATTTTGAT GATGACGATG TTGACCTTTC CAGGGAAAAG 
GCCGAGTTAA GAAAGGGCAA AGAAAGCAAG GATTCCGAAG CCAAAGTTAC CTGCCGCCCA 
GAACCAAACT CAAGCCAGCA GGCAGCTAGT AAGTCACAAG CCAGTATAAA ACATCCAGCA 
AACAGAGCAC AGTCCAAACC AGTGCTGCAG AAACAGCCCA CTTTCCCCCA GTCCTCCAAA 
GACGGACCAG ATAGAGGGGC AGCAACTGAC GAAAAACTGC AGAATTTTGC TATTGAAAAT 
ACTCCAGTTT GCTTTTCTCG AAATTCCTCT CTGAGTTCCC TTAGTGACAT TGACCAGGAA 
AACAACAATA ACAAAGAAAG TGAACCAATC AAAGAAGCTG AACCTGCCAA CTCACAAGGA 
GAGCCCAGTA AGCCTCAGGC ATCCGGGTAT GCTCCCAAGT CCTTCCACGT CGAAGACACC 
CCTGTCTGTT TCTCAAGAAA CAGCTCTCTC AGTTCTCTTA GCATTGACTC TGAGGACGAC 
CTGTTACAGG AGTGTATAAG TTCTGCCATG CCAAAAAAGA AAAGGCCTTC AAGACTCAAG 
AGTGAGAGCG AAAAGCAGAG CCCTAGAAAA GTGGGTGGCA TATTAGCTGA AGACCTGACG 
CTTGATTTGA AAGATCTACA GAGGCCAGAT TCAGAACACG CTTTCTCCCC CGACTCAGAA 
AATTTTGACT GGAAAGCTAT TCAGGAAGGC GCAAACTCCA TAGTAAGTAG TTTGCACCAA 
GCTGCTGCAG CCGCCGCGTG CTTATCTAGA CAAGCGTCAT CCGACTCAGA TTCCATTCTG 
TCACTAAAGT CCGGCATTTC TCTGGGATCG CCTTTTCATC TTACACCTGA TCAAGAGGAA 
AAGCCATTCA CAAGCAATAA AGGCCCAAGA ATTCTCAAAC CTGGAGAGAA AAGCACATTA 
GAAGCAAAAA AAATAGAATC TGAAAACAAA GGAATCAAAG GCGGGAAAAA GGTTTATAAA 
AGCTTGATTA CGGGAAAGAT TCGCTCCAAT TCAGAAATTT CCAGCCAAAT GAAACAACCC 
CTCCCGACAA ACATGCCTTC AATCTCAAGA GGCAGGACGA TGATTCACAT CCCAGGGCTT 
CGGAATAGCT CCTCTAGTAC AAGCCCTGTC TCTAAGAAAG GCCCACCCCT CAAGACTCCA 
GCCTCTAAAA GCCCCAGTGA AGGGCCGGGA GCTACCACTT CTCCTCGAGG AACTAAGCCA 
GCAGGAAAGT CAGAGCTTAG CCCTATCACC AGGCAAACTT CCCAAATCAG TGGGTCAAAT 
AAGGGGTCTT CTAGATCAGG ATCTAGAGAC TCCACTCCCT CAAGACCTAC ACAGCAACCA 
TTAAGTAGGC CAATGCAGTC TCCAGGGCGA AACTCAATTT CCCCTGGTAG AAATGGAATA 
AGCCCTCCTA ACAAACTGTC TCAGCTGCCC AGAACATCAT CTCCCAGTAC TGCTTCAACT 
AAGTCCTCCG GTTCTGGGAA AATGTCATAT ACATCCCCAG GTAGACAGCT GAGCCAACAA 
AATCTTACCA AACAAGCAAG TTTATCCAAG AATGCCAGCA GTATCCCCAG AAGTGAGTCG 
GCATCTAAAG GACTGAATCA GATGAGTAAC GGCAATGGGT CAAATAAAAA GGTAGAACTT 
TCTAGAATGT CTTCAACTAA ATCAAGTGGA AGTGAATCAG ACAGATCAGA AAGGCCTGCA 
TTAGTACGCC AGTCTACTTT CATCAAAGAA GCCCCAAGCC CAACCCTGAG GAGGAAACTG 
GAGGAATCTG CCTCATTTGA ATCCCTTTCT CCATCTTCTA GACCAGATTC TCCCACCAGG 
TCGCAGGCAC AGACCCCAGT TTTAAGCCCT TCCCTTCCTG ATATGTCTCT GTCCACACAT 
CCATCTGTTC AGGCAGGTGG GTGGCGAAAG CTCCCGCCTA ATCTCAGCCC CACTATCGAG 
TATAATGACG GAAGGCCCAC AAAACGGCAT GATATTGCAC GCTCCCATTC TGAAAGTCCT 
TCCAGACTAC CAATCAACCG GGCGGGAACC TGGAAGCGTG AACACAGCAA ACATTCCTCG 
TCCCTTCCTC GAGTGAGTAC TTGGAGAAGA ACTGGAAGCT CATCTTCTAT TCTTTCTGCT 
TCATCAGAGT CCAGTGAAAA AGCAAAAAGT GAGGATGAAA GGCATGTGAG CTCCATGCCA 
GCACCCAGAC AGATGAAGGA AAACCAGGTG CCCACCAAAG GAACATGGAG GAAAATCAAG 
GAAAGTGACA TTTCTCCCAC AGGCATGGCT TCTCAGAGCG CTTCCTCAGG TGCTGCCAGT 
GGTGCTGAAT CCAAGCCTCT GATCTATCAG ATGGCACCTC CTGTCTCTAA AACAGAGGAT 
GTTTGGGTGA GAATTGAGGA CTGCCCCATT AACAACCCTA GATCTGGACG GTCCCCCACA 
GGCAACACCC CCCCAGTGAT TGACAGTGTT TCAGAGAAGG GAAGTTCAAG CATTAAAGAT 
TCAAAAGACA CCCATGGGAA ACAGAGTGTG GGCAGTGGCA GTCCTGTGCA AACCGTGGGT 
CTGGAAACCC GCCTCAACTC CTTTGTTCAG GTAGAGGCCC CAGAACAGAA AGGAACTGAG 
GCAAAACCAG GACAGAGTAA CCCAGTCTCT ATAGCAGAGA CTGCTGAGAC GTGTATAGCA 
GAGCGTACCC CTTTCAGTTC CAGTAGCTCC AGCAAGCACA GCTCACCTAG CGGGACTGTT 
GCTGCCAGAG TGACACCTTT TAATTACAAC CCTAGCCCTA GGAAGAGCAG CGCAGACAGC 
ACTTCAGCCC GGCCGTCTCA GATCCCTACG CCAGTGAGCA CCAACACGAA GAAGAGAGAT 
TCGAAGACTG ACAGCACAGA ATCCAGTGGA GCCCAAAGTC CTAAACGCCA TTCCGGGTCT 
TACCTCGTGA CGTCTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250295.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106155.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X27, mRNA.

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CloneID	OMu87333
Clone ID Related Accession (Same CDS sequence)	XM_030250295.1 , XM_030250296.1
Accession Version	XM_030250295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGGGGACTG CAGGCCATTG CAGAGTTATT GCAGGTGGAC 
TGTGAGATGT ATGGGCTTAC TAATGACCAC TACAGTGTTA CTTTAAGACG GTATGCTGGA 
ATGGCTTTGA CAAACTTGAC CTTTGGAGAT GTTGCCAACA AGGCTACGCT GTGTTCTATG 
AAAGGCTGCA TGAGAGCACT TGTGGCCCAG TTAAAATCTG AGAGTGAAGA CTTACAGCAG 
GTTATTGCAA GTGTTTTGAG GAATTTGTCT TGGCGAGCAG ATGTAAATAG CAAAAAGACG 
TTGAGAGAAG TTGGAAGTGT GAAAGCATTG ATGGAATGTG CTTTGGAAGT TAAAAAGGAA 
TCAACCCTCA AAAGCGTTTT GAGTGCCTTA TGGAACCTGT CTGCACACTG CACTGAGAAT 
AAGGCTGACA TCTGTGCTGT GGATGGAGCA CTGGCATTTC TGGTTGGCAC CCTCACTTAC 
CGGAGCCAGA CAAATACTTT AGCCATTATT GAAAGTGGAG GTGGGATATT ACGGAATGTG 
TCCAGCTTGA TAGCTACAAA CGAAGACCAC AGGCAAATCC TAAGAGAGAA CAATTGCCTA 
CAAACTTTAT TACAGCACTT GAAATCTCAC AGCTTGACAA TAGTCAGTAA TGCATGTGGA 
ACTTTGTGGA ATCTCTCAGC AAGAAATCCT AAAGACCAGG AAGCCTTGTG GGACATGGGG 
GCAGTGAGCA TGCTCAAGAA CCTCATTCAT TCCAAGCACA AAATGATTGC CATGGGAAGT 
GCAGCAGCTT TAAGGAATCT CATGGCAAAC AGACCTGCAA AGTATAAGGA TGCCAATATC 
ATGTCTCCCG GCTCAAGTCT GCCATCCCTT CACGTTAGGA AACAGAAAGC TCTAGAAGCT 
GAGCTAGATG CTCAGCATTT ATCAGAAACC TTCGACAACA TTGACAACCT AAGTCCCAAG 
GCCTCTCACC GGAGTAAGCA GAGACACAAG CAGAATCTTT ATGGTGACTA TGCTTTTGAC 
GCCAATCGAC ATGATGATAG TAGGTCAGAC AATTTCAATA CTGGAAACAT GACTGTTCTT 
TCACCATATT TAAATACTAC GGTATTGCCC AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC 
AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG GAGAGAGAGC GAGGTATTGG CCTCAGTGCT 
TACCATCCAA CAACAGAAAA TGCAGGAACC TCATCAAAAC GAGGTCTGCA GATCACTACC 
ACTGCAGCCC AGATAGCCAA AGTTATGGAA GAAGTATCAG CCATTCATAC CTCCCAGGAC 
GACAGAAGTT CTGCTTCTAC CACCGAGTTC CATTGTGTGG CAGACGACAG GAGTGCGGCA 
CGAAGAAGCT CTGCCTCCCA CACACACTCA AACACATACA ACTTCACTAA GTCGGAAAAT 
TCAAATAGGA CATGCTCTAT GCCTTATGCC AAAGTGGAAT ATAAACGATC TTCAAATGAC 
AGTTTAAATA GTGTCACTAG TAGTGATGGA TATGGTAAAA GAGGCCAAAT GAAACCCTCA 
GTTGAATCCT ATTCTGAAGA TGATGAAAGT AAATTTTGCA GTTATGGTCA GTATCCAGCT 
GACCTAGCCC ATAAGATACA CAGTGCAAAT CATATGGATG ATAATGATGG AGAACTGGAT 
ACACCAATAA ATTACAGTCT TAAATATTCA GATGAGCAGT TGAACTCAGG AAGGCAGAGT 
CCCTCACAGA ATGAAAGGTG GGCAAGACCA AAGCATGTGA TAGAAGATGA AATAAAGCAA 
AACGAGCAAA GACAAGCAAG AAGCCAGAAC ACCAGTTATC CTGTCTATTC TGAGAATACC 
GATGACAAAC ACCTCAAATT CCAACCACAT TTTGGACAAC AAGAATGTGT TTCCCCATAT 
AGGTCAAGGG GAACCAGTGG TTCAGAAACA AATCGAATGG GTTCTAGTCA TGCAATTAAT 
CAAAATGTAA ACCAGTCTCT GTGTCAGGAA GATGATTATG AAGATGATAA ACCTACCAAC 
TACAGTGAAC GTTATTCTGA GGAAGAACAA CATGAAGAAG AAGAAGAGAG ACCGACAAAT 
TATAGCATAA AATATAATGA AGAGAAACAT CATGTGGATC AGCCTATTGA TTATAGTTTA 
AAATATGCCA CTGACATTTC TTCCTCACAA AAACCATCAT TTTCATTCTC AAAGAATTCA 
TCAGCACAAA GCACTAAACC TGAACATCTC TCTCCAAGCA GCGAGAATAC AGCTGTACCT 
CCATCTAATG CCAAAAGGCA GAATCAGCTG CGTCCAAGTT CAGCACAAAG AAATGGCCAG 
ACTCAAAAAG GCACTACTTG CAAAGTCCCC TCCATCAACC AAGAAACAAT ACAGACTTAC 
TGCGTAGAAG ACACCCCAAT ATGTTTTTCA AGGTGCAGTT CATTATCATC ACTGTCATCA 
GCTGACGATG AAATAGGATG TGATCAGACA ACACAGGAAG CAGATTCTGC TAATACTCTG 
CAGACAGCAG AAGTAAAAGA GAATGATGTA ACTCGGTCAG CTGAAGATCC TGCAACTGAA 
GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC AAACCCAGCC GACTCCAGGC TTCTGGCTTA 
TCTTCAGAAT CAACCAGGCA TAATAAAGCT GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC 
TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT CCCCCAGAAC ACTATGTCCA GGAGACTCCG 
CTCGTATTCA GCAGGTGTAC TTCTGTCAGC TCCCTTGACA GTTTTGAGAG TCGCTCCATT 
GCCAGCTCTG TTCAGAGTGA GCCATGTAGT GGAATGGTGA GTGGCATCAT AAGCCCCAGT 
GACCTTCCAG ATAGTCCTGG GCAGACCATG CCACCAAGCA GAAGCAAAAC CCCTCCACCT 
CCTCCACAGA CAGTGCAGGC CAAGAGAGAG GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG 
AAGAGAGAGA GTGGGCCTAA GCAGACTGCT GTAAATGCTG CCGTGCAGAG GGTGCAGGTC 
CTTCCAGACG TGGATACTTT GTTACACTTC GCCACAGAAA GTACTCCAGA CGGGTTTTCT 
TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG GATGAGCCAT TTATACAGAA AGATGTAGAA 
TTAAGAATCA TGCCTCCAGT TCAGGAAAAC GACAATGGGA ATGAAACTGA ATCAGAACAG 
CCTGAGGAAT CAAATGAAAA CCAGGATAAA GAGGTAGAAA AGCCTGACTC TGAAAAAGAC 
TTATTAGATG ATTCTGATGA CGATGATATT GAAATATTAG AAGAATGTAT TATTTCAGCC 
ATGCCAACAA AGTCATCACG CAAAGCCAAA AAACTAGCCC AGACTGCTTC AAAATTACCT 
CCACCTGTGG CAAGGAAACC AAGTCAGCTA CCTGTGTATA AACTTCTGCC AGCACAGAAT 
AGGCTGCAGG CACAAAAACA TGTTAGCTTT ACACCAGGGG ATGATGTGCC CCGGGTGTAC 
TGTGTAGAAG GGACACCTAT AAACTTTTCC ACAGCAACGT CTCTAAGTGA TCTGACAATA 
GAGTCCCCTC CAAATGAATT GGCTACTGGA GATGGGGTCA GAGCGGGTAT ACAGTCAGGT 
GAATTTGAAA AACGAGATAC CATTCCTACA GAAGGCAGAA GTACAGATGA TGCTCAGCGA 
GGAAAAATCT CATCTATAGT TACACCAGAC CTGGATGACA ACAAAGCAGA GGAAGGAGAT 
ATTCTTGCAG AATGTATCAA TTCTGCTATG CCCAAAGGAA AAAGCCACAA GCCTTTCCGA 
GTGAAAAAGA TAATGGACCA AGTCCAACAA GCATCCTCGA CTTCATCTGG AGCTAACAAA 
AATCAAGTAG ACACTAAGAA AAAGAAGCCT ACTTCACCAG TAAAGCCCAT GCCACAAAAT 
ACTGAATATA GAACGCGTGT GAGAAAGAAT ACAGACTCAA AAGTTAATGT AAATACTGAA 
GAAACTTTCT CAGACAACAA AGACTCAAAG AAACCAAGCT TACAAACCAA TGCCAAGGCC 
TTCAATGAAA AGCTACCTAA CAATGAAGAC AGAGTGCGGG GGAGCTTCGC CTTGGACTCA 
CCGCATCACT ACACCCCTAT TGAGGGGACG CCGTACTGCT TTTCCCGAAA TGACTCCTTG 
AGTTCTCTGG ATTTTGATGA TGACGATGTT GACCTTTCCA GGGAAAAGGC CGAGTTAAGA 
AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC AAAGTTACCT GCCGCCCAGA ACCAAACTCA 
AGCCAGCAGG CAGCTAGTAA GTCACAAGCC AGTATAAAAC ATCCAGCAAA CAGAGCACAG 
TCCAAACCAG TGCTGCAGAA ACAGCCCACT TTCCCCCAGT CCTCCAAAGA CGGACCAGAT 
AGAGGGGCAG CAACTGACGA AAAACTGCAG AATTTTGCTA TTGAAAATAC TCCAGTTTGC 
TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT AGTGACATTG ACCAGGAAAA CAACAATAAC 
AAAGAAAGTG AACCAATCAA AGAAGCTGAA CCTGCCAACT CACAAGGAGA GCCCAGTAAG 
CCTCAGGCAT CCGGGTATGC TCCCAAGTCC TTCCACGTCG AAGACACCCC TGTCTGTTTC 
TCAAGAAACA GCTCTCTCAG TTCTCTTAGC ATTGACTCTG AGGACGACCT GTTACAGGAG 
TGTATAAGTT CTGCCATGCC AAAAAAGAAA AGGCCTTCAA GACTCAAGAG TGAGAGCGAA 
AAGCAGAGCC CTAGAAAAGT GGGTGGCATA TTAGCTGAAG ACCTGACGCT TGATTTGAAA 
GATCTACAGA GGCCAGATTC AGAACACGCT TTCTCCCCCG ACTCAGAAAA TTTTGACTGG 
AAAGCTATTC AGGAAGGCGC AAACTCCATA GTAAGTAGTT TGCACCAAGC TGCTGCAGCC 
GCCGCGTGCT TATCTAGACA AGCGTCATCC GACTCAGATT CCATTCTGTC ACTAAAGTCC 
GGCATTTCTC TGGGATCGCC TTTTCATCTT ACACCTGATC AAGAGGAAAA GCCATTCACA 
AGCAATAAAG GCCCAAGAAT TCTCAAACCT GGAGAGAAAA GCACATTAGA AGCAAAAAAA 
ATAGAATCTG AAAACAAAGG AATCAAAGGC GGGAAAAAGG TTTATAAAAG CTTGATTACG 
GGAAAGATTC GCTCCAATTC AGAAATTTCC AGCCAAATGA AACAACCCCT CCCGACAAAC 
ATGCCTTCAA TCTCAAGAGG CAGGACGATG ATTCACATCC CAGGGCTTCG GAATAGCTCC 
TCTAGTACAA GCCCTGTCTC TAAGAAAGGC CCACCCCTCA AGACTCCAGC CTCTAAAAGC 
CCCAGTGAAG GGCCGGGAGC TACCACTTCT CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA 
GAGCTTAGCC CTATCACCAG GCAAACTTCC CAAATCAGTG GGTCAAATAA GGGGTCTTCT 
AGATCAGGAT CTAGAGACTC CACTCCCTCA AGACCTACAC AGCAACCATT AAGTAGGCCA 
ATGCAGTCTC CAGGGCGAAA CTCAATTTCC CCTGGTAGAA ATGGAATAAG CCCTCCTAAC 
AAACTGTCTC AGCTGCCCAG AACATCATCT CCCAGTACTG CTTCAACTAA GTCCTCCGGT 
TCTGGGAAAA TGTCATATAC ATCCCCAGGT AGACAGCTGA GCCAACAAAA TCTTACCAAA 
CAAGCAAGTT TATCCAAGAA TGCCAGCAGT ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA 
CTGAATCAGA TGAGTAACGG CAATGGGTCA AATAAAAAGG TAGAACTTTC TAGAATGTCT 
TCAACTAAAT CAAGTGGAAG TGAATCAGAC AGATCAGAAA GGCCTGCATT AGTACGCCAG 
TCTACTTTCA TCAAAGAAGC CCCAAGCCCA ACCCTGAGGA GGAAACTGGA GGAATCTGCC 
TCATTTGAAT CCCTTTCTCC ATCTTCTAGA CCAGATTCTC CCACCAGGTC GCAGGCACAG 
ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT ATGTCTCTGT CCACACATCC ATCTGTTCAG 
GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT CTCAGCCCCA CTATCGAGTA TAATGACGGA 
AGGCCCACAA AACGGCATGA TATTGCACGC TCCCATTCTG AAAGTCCTTC CAGACTACCA 
ATCAACCGGG CGGGAACCTG GAAGCGTGAA CACAGCAAAC ATTCCTCGTC CCTTCCTCGA 
GTGAGTACTT GGAGAAGAAC TGGAAGCTCA TCTTCTATTC TTTCTGCTTC ATCAGAGTCC 
AGTGAAAAAG CAAAAAGTGA GGATGAAAGG CATGTGAGCT CCATGCCAGC ACCCAGACAG 
ATGAAGGAAA ACCAGGTGCC CACCAAAGGA ACATGGAGGA AAATCAAGGA AAGTGACATT 
TCTCCCACAG GCATGGCTTC TCAGAGCGCT TCCTCAGGTG CTGCCAGTGG TGCTGAATCC 
AAGCCTCTGA TCTATCAGAT GGCACCTCCT GTCTCTAAAA CAGAGGATGT TTGGGTGAGA 
ATTGAGGACT GCCCCATTAA CAACCCTAGA TCTGGACGGT CCCCCACAGG CAACACCCCC 
CCAGTGATTG ACAGTGTTTC AGAGAAGGGA AGTTCAAGCA TTAAAGATTC AAAAGACACC 
CATGGGAAAC AGAGTGTGGG CAGTGGCAGT CCTGTGCAAA CCGTGGGTCT GGAAACCCGC 
CTCAACTCCT TTGTTCAGGT AGAGGCCCCA GAACAGAAAG GAACTGAGGC AAAACCAGGA 
CAGAGTAACC CAGTCTCTAT AGCAGAGACT GCTGAGACGT GTATAGCAGA GCGTACCCCT 
TTCAGTTCCA GTAGCTCCAG CAAGCACAGC TCACCTAGCG GGACTGTTGC TGCCAGAGTG 
ACACCTTTTA ATTACAACCC TAGCCCTAGG AAGAGCAGCG CAGACAGCAC TTCAGCCCGG 
CCGTCTCAGA TCCCTACGCC AGTGAGCACC AACACGAAGA AGAGAGATTC GAAGACTGAC 
AGCACAGAAT CCAGTGGAGC CCAAAGTCCT AAACGCCATT CCGGGTCTTA CCTCGTGACG 
TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106155.1
CDS	399..8447
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250295.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250295.1

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGGGGACTG CAGGCCATTG CAGAGTTATT GCAGGTGGAC 
TGTGAGATGT ATGGGCTTAC TAATGACCAC TACAGTGTTA CTTTAAGACG GTATGCTGGA 
ATGGCTTTGA CAAACTTGAC CTTTGGAGAT GTTGCCAACA AGGCTACGCT GTGTTCTATG 
AAAGGCTGCA TGAGAGCACT TGTGGCCCAG TTAAAATCTG AGAGTGAAGA CTTACAGCAG 
GTTATTGCAA GTGTTTTGAG GAATTTGTCT TGGCGAGCAG ATGTAAATAG CAAAAAGACG 
TTGAGAGAAG TTGGAAGTGT GAAAGCATTG ATGGAATGTG CTTTGGAAGT TAAAAAGGAA 
TCAACCCTCA AAAGCGTTTT GAGTGCCTTA TGGAACCTGT CTGCACACTG CACTGAGAAT 
AAGGCTGACA TCTGTGCTGT GGATGGAGCA CTGGCATTTC TGGTTGGCAC CCTCACTTAC 
CGGAGCCAGA CAAATACTTT AGCCATTATT GAAAGTGGAG GTGGGATATT ACGGAATGTG 
TCCAGCTTGA TAGCTACAAA CGAAGACCAC AGGCAAATCC TAAGAGAGAA CAATTGCCTA 
CAAACTTTAT TACAGCACTT GAAATCTCAC AGCTTGACAA TAGTCAGTAA TGCATGTGGA 
ACTTTGTGGA ATCTCTCAGC AAGAAATCCT AAAGACCAGG AAGCCTTGTG GGACATGGGG 
GCAGTGAGCA TGCTCAAGAA CCTCATTCAT TCCAAGCACA AAATGATTGC CATGGGAAGT 
GCAGCAGCTT TAAGGAATCT CATGGCAAAC AGACCTGCAA AGTATAAGGA TGCCAATATC 
ATGTCTCCCG GCTCAAGTCT GCCATCCCTT CACGTTAGGA AACAGAAAGC TCTAGAAGCT 
GAGCTAGATG CTCAGCATTT ATCAGAAACC TTCGACAACA TTGACAACCT AAGTCCCAAG 
GCCTCTCACC GGAGTAAGCA GAGACACAAG CAGAATCTTT ATGGTGACTA TGCTTTTGAC 
GCCAATCGAC ATGATGATAG TAGGTCAGAC AATTTCAATA CTGGAAACAT GACTGTTCTT 
TCACCATATT TAAATACTAC GGTATTGCCC AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC 
AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG GAGAGAGAGC GAGGTATTGG CCTCAGTGCT 
TACCATCCAA CAACAGAAAA TGCAGGAACC TCATCAAAAC GAGGTCTGCA GATCACTACC 
ACTGCAGCCC AGATAGCCAA AGTTATGGAA GAAGTATCAG CCATTCATAC CTCCCAGGAC 
GACAGAAGTT CTGCTTCTAC CACCGAGTTC CATTGTGTGG CAGACGACAG GAGTGCGGCA 
CGAAGAAGCT CTGCCTCCCA CACACACTCA AACACATACA ACTTCACTAA GTCGGAAAAT 
TCAAATAGGA CATGCTCTAT GCCTTATGCC AAAGTGGAAT ATAAACGATC TTCAAATGAC 
AGTTTAAATA GTGTCACTAG TAGTGATGGA TATGGTAAAA GAGGCCAAAT GAAACCCTCA 
GTTGAATCCT ATTCTGAAGA TGATGAAAGT AAATTTTGCA GTTATGGTCA GTATCCAGCT 
GACCTAGCCC ATAAGATACA CAGTGCAAAT CATATGGATG ATAATGATGG AGAACTGGAT 
ACACCAATAA ATTACAGTCT TAAATATTCA GATGAGCAGT TGAACTCAGG AAGGCAGAGT 
CCCTCACAGA ATGAAAGGTG GGCAAGACCA AAGCATGTGA TAGAAGATGA AATAAAGCAA 
AACGAGCAAA GACAAGCAAG AAGCCAGAAC ACCAGTTATC CTGTCTATTC TGAGAATACC 
GATGACAAAC ACCTCAAATT CCAACCACAT TTTGGACAAC AAGAATGTGT TTCCCCATAT 
AGGTCAAGGG GAACCAGTGG TTCAGAAACA AATCGAATGG GTTCTAGTCA TGCAATTAAT 
CAAAATGTAA ACCAGTCTCT GTGTCAGGAA GATGATTATG AAGATGATAA ACCTACCAAC 
TACAGTGAAC GTTATTCTGA GGAAGAACAA CATGAAGAAG AAGAAGAGAG ACCGACAAAT 
TATAGCATAA AATATAATGA AGAGAAACAT CATGTGGATC AGCCTATTGA TTATAGTTTA 
AAATATGCCA CTGACATTTC TTCCTCACAA AAACCATCAT TTTCATTCTC AAAGAATTCA 
TCAGCACAAA GCACTAAACC TGAACATCTC TCTCCAAGCA GCGAGAATAC AGCTGTACCT 
CCATCTAATG CCAAAAGGCA GAATCAGCTG CGTCCAAGTT CAGCACAAAG AAATGGCCAG 
ACTCAAAAAG GCACTACTTG CAAAGTCCCC TCCATCAACC AAGAAACAAT ACAGACTTAC 
TGCGTAGAAG ACACCCCAAT ATGTTTTTCA AGGTGCAGTT CATTATCATC ACTGTCATCA 
GCTGACGATG AAATAGGATG TGATCAGACA ACACAGGAAG CAGATTCTGC TAATACTCTG 
CAGACAGCAG AAGTAAAAGA GAATGATGTA ACTCGGTCAG CTGAAGATCC TGCAACTGAA 
GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC AAACCCAGCC GACTCCAGGC TTCTGGCTTA 
TCTTCAGAAT CAACCAGGCA TAATAAAGCT GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC 
TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT CCCCCAGAAC ACTATGTCCA GGAGACTCCG 
CTCGTATTCA GCAGGTGTAC TTCTGTCAGC TCCCTTGACA GTTTTGAGAG TCGCTCCATT 
GCCAGCTCTG TTCAGAGTGA GCCATGTAGT GGAATGGTGA GTGGCATCAT AAGCCCCAGT 
GACCTTCCAG ATAGTCCTGG GCAGACCATG CCACCAAGCA GAAGCAAAAC CCCTCCACCT 
CCTCCACAGA CAGTGCAGGC CAAGAGAGAG GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG 
AAGAGAGAGA GTGGGCCTAA GCAGACTGCT GTAAATGCTG CCGTGCAGAG GGTGCAGGTC 
CTTCCAGACG TGGATACTTT GTTACACTTC GCCACAGAAA GTACTCCAGA CGGGTTTTCT 
TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG GATGAGCCAT TTATACAGAA AGATGTAGAA 
TTAAGAATCA TGCCTCCAGT TCAGGAAAAC GACAATGGGA ATGAAACTGA ATCAGAACAG 
CCTGAGGAAT CAAATGAAAA CCAGGATAAA GAGGTAGAAA AGCCTGACTC TGAAAAAGAC 
TTATTAGATG ATTCTGATGA CGATGATATT GAAATATTAG AAGAATGTAT TATTTCAGCC 
ATGCCAACAA AGTCATCACG CAAAGCCAAA AAACTAGCCC AGACTGCTTC AAAATTACCT 
CCACCTGTGG CAAGGAAACC AAGTCAGCTA CCTGTGTATA AACTTCTGCC AGCACAGAAT 
AGGCTGCAGG CACAAAAACA TGTTAGCTTT ACACCAGGGG ATGATGTGCC CCGGGTGTAC 
TGTGTAGAAG GGACACCTAT AAACTTTTCC ACAGCAACGT CTCTAAGTGA TCTGACAATA 
GAGTCCCCTC CAAATGAATT GGCTACTGGA GATGGGGTCA GAGCGGGTAT ACAGTCAGGT 
GAATTTGAAA AACGAGATAC CATTCCTACA GAAGGCAGAA GTACAGATGA TGCTCAGCGA 
GGAAAAATCT CATCTATAGT TACACCAGAC CTGGATGACA ACAAAGCAGA GGAAGGAGAT 
ATTCTTGCAG AATGTATCAA TTCTGCTATG CCCAAAGGAA AAAGCCACAA GCCTTTCCGA 
GTGAAAAAGA TAATGGACCA AGTCCAACAA GCATCCTCGA CTTCATCTGG AGCTAACAAA 
AATCAAGTAG ACACTAAGAA AAAGAAGCCT ACTTCACCAG TAAAGCCCAT GCCACAAAAT 
ACTGAATATA GAACGCGTGT GAGAAAGAAT ACAGACTCAA AAGTTAATGT AAATACTGAA 
GAAACTTTCT CAGACAACAA AGACTCAAAG AAACCAAGCT TACAAACCAA TGCCAAGGCC 
TTCAATGAAA AGCTACCTAA CAATGAAGAC AGAGTGCGGG GGAGCTTCGC CTTGGACTCA 
CCGCATCACT ACACCCCTAT TGAGGGGACG CCGTACTGCT TTTCCCGAAA TGACTCCTTG 
AGTTCTCTGG ATTTTGATGA TGACGATGTT GACCTTTCCA GGGAAAAGGC CGAGTTAAGA 
AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC AAAGTTACCT GCCGCCCAGA ACCAAACTCA 
AGCCAGCAGG CAGCTAGTAA GTCACAAGCC AGTATAAAAC ATCCAGCAAA CAGAGCACAG 
TCCAAACCAG TGCTGCAGAA ACAGCCCACT TTCCCCCAGT CCTCCAAAGA CGGACCAGAT 
AGAGGGGCAG CAACTGACGA AAAACTGCAG AATTTTGCTA TTGAAAATAC TCCAGTTTGC 
TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT AGTGACATTG ACCAGGAAAA CAACAATAAC 
AAAGAAAGTG AACCAATCAA AGAAGCTGAA CCTGCCAACT CACAAGGAGA GCCCAGTAAG 
CCTCAGGCAT CCGGGTATGC TCCCAAGTCC TTCCACGTCG AAGACACCCC TGTCTGTTTC 
TCAAGAAACA GCTCTCTCAG TTCTCTTAGC ATTGACTCTG AGGACGACCT GTTACAGGAG 
TGTATAAGTT CTGCCATGCC AAAAAAGAAA AGGCCTTCAA GACTCAAGAG TGAGAGCGAA 
AAGCAGAGCC CTAGAAAAGT GGGTGGCATA TTAGCTGAAG ACCTGACGCT TGATTTGAAA 
GATCTACAGA GGCCAGATTC AGAACACGCT TTCTCCCCCG ACTCAGAAAA TTTTGACTGG 
AAAGCTATTC AGGAAGGCGC AAACTCCATA GTAAGTAGTT TGCACCAAGC TGCTGCAGCC 
GCCGCGTGCT TATCTAGACA AGCGTCATCC GACTCAGATT CCATTCTGTC ACTAAAGTCC 
GGCATTTCTC TGGGATCGCC TTTTCATCTT ACACCTGATC AAGAGGAAAA GCCATTCACA 
AGCAATAAAG GCCCAAGAAT TCTCAAACCT GGAGAGAAAA GCACATTAGA AGCAAAAAAA 
ATAGAATCTG AAAACAAAGG AATCAAAGGC GGGAAAAAGG TTTATAAAAG CTTGATTACG 
GGAAAGATTC GCTCCAATTC AGAAATTTCC AGCCAAATGA AACAACCCCT CCCGACAAAC 
ATGCCTTCAA TCTCAAGAGG CAGGACGATG ATTCACATCC CAGGGCTTCG GAATAGCTCC 
TCTAGTACAA GCCCTGTCTC TAAGAAAGGC CCACCCCTCA AGACTCCAGC CTCTAAAAGC 
CCCAGTGAAG GGCCGGGAGC TACCACTTCT CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA 
GAGCTTAGCC CTATCACCAG GCAAACTTCC CAAATCAGTG GGTCAAATAA GGGGTCTTCT 
AGATCAGGAT CTAGAGACTC CACTCCCTCA AGACCTACAC AGCAACCATT AAGTAGGCCA 
ATGCAGTCTC CAGGGCGAAA CTCAATTTCC CCTGGTAGAA ATGGAATAAG CCCTCCTAAC 
AAACTGTCTC AGCTGCCCAG AACATCATCT CCCAGTACTG CTTCAACTAA GTCCTCCGGT 
TCTGGGAAAA TGTCATATAC ATCCCCAGGT AGACAGCTGA GCCAACAAAA TCTTACCAAA 
CAAGCAAGTT TATCCAAGAA TGCCAGCAGT ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA 
CTGAATCAGA TGAGTAACGG CAATGGGTCA AATAAAAAGG TAGAACTTTC TAGAATGTCT 
TCAACTAAAT CAAGTGGAAG TGAATCAGAC AGATCAGAAA GGCCTGCATT AGTACGCCAG 
TCTACTTTCA TCAAAGAAGC CCCAAGCCCA ACCCTGAGGA GGAAACTGGA GGAATCTGCC 
TCATTTGAAT CCCTTTCTCC ATCTTCTAGA CCAGATTCTC CCACCAGGTC GCAGGCACAG 
ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT ATGTCTCTGT CCACACATCC ATCTGTTCAG 
GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT CTCAGCCCCA CTATCGAGTA TAATGACGGA 
AGGCCCACAA AACGGCATGA TATTGCACGC TCCCATTCTG AAAGTCCTTC CAGACTACCA 
ATCAACCGGG CGGGAACCTG GAAGCGTGAA CACAGCAAAC ATTCCTCGTC CCTTCCTCGA 
GTGAGTACTT GGAGAAGAAC TGGAAGCTCA TCTTCTATTC TTTCTGCTTC ATCAGAGTCC 
AGTGAAAAAG CAAAAAGTGA GGATGAAAGG CATGTGAGCT CCATGCCAGC ACCCAGACAG 
ATGAAGGAAA ACCAGGTGCC CACCAAAGGA ACATGGAGGA AAATCAAGGA AAGTGACATT 
TCTCCCACAG GCATGGCTTC TCAGAGCGCT TCCTCAGGTG CTGCCAGTGG TGCTGAATCC 
AAGCCTCTGA TCTATCAGAT GGCACCTCCT GTCTCTAAAA CAGAGGATGT TTGGGTGAGA 
ATTGAGGACT GCCCCATTAA CAACCCTAGA TCTGGACGGT CCCCCACAGG CAACACCCCC 
CCAGTGATTG ACAGTGTTTC AGAGAAGGGA AGTTCAAGCA TTAAAGATTC AAAAGACACC 
CATGGGAAAC AGAGTGTGGG CAGTGGCAGT CCTGTGCAAA CCGTGGGTCT GGAAACCCGC 
CTCAACTCCT TTGTTCAGGT AGAGGCCCCA GAACAGAAAG GAACTGAGGC AAAACCAGGA 
CAGAGTAACC CAGTCTCTAT AGCAGAGACT GCTGAGACGT GTATAGCAGA GCGTACCCCT 
TTCAGTTCCA GTAGCTCCAG CAAGCACAGC TCACCTAGCG GGACTGTTGC TGCCAGAGTG 
ACACCTTTTA ATTACAACCC TAGCCCTAGG AAGAGCAGCG CAGACAGCAC TTCAGCCCGG 
CCGTCTCAGA TCCCTACGCC AGTGAGCACC AACACGAAGA AGAGAGATTC GAAGACTGAC 
AGCACAGAAT CCAGTGGAGC CCAAAGTCCT AAACGCCATT CCGGGTCTTA CCTCGTGACG 
TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apc ( XM_030250296.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106156.1 Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X28, mRNA.

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CloneID	OMu87333
Clone ID Related Accession (Same CDS sequence)	XM_030250295.1 , XM_030250296.1
Accession Version	XM_030250296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	adenomatous polyposis coli protein isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGGGGACTG CAGGCCATTG CAGAGTTATT GCAGGTGGAC 
TGTGAGATGT ATGGGCTTAC TAATGACCAC TACAGTGTTA CTTTAAGACG GTATGCTGGA 
ATGGCTTTGA CAAACTTGAC CTTTGGAGAT GTTGCCAACA AGGCTACGCT GTGTTCTATG 
AAAGGCTGCA TGAGAGCACT TGTGGCCCAG TTAAAATCTG AGAGTGAAGA CTTACAGCAG 
GTTATTGCAA GTGTTTTGAG GAATTTGTCT TGGCGAGCAG ATGTAAATAG CAAAAAGACG 
TTGAGAGAAG TTGGAAGTGT GAAAGCATTG ATGGAATGTG CTTTGGAAGT TAAAAAGGAA 
TCAACCCTCA AAAGCGTTTT GAGTGCCTTA TGGAACCTGT CTGCACACTG CACTGAGAAT 
AAGGCTGACA TCTGTGCTGT GGATGGAGCA CTGGCATTTC TGGTTGGCAC CCTCACTTAC 
CGGAGCCAGA CAAATACTTT AGCCATTATT GAAAGTGGAG GTGGGATATT ACGGAATGTG 
TCCAGCTTGA TAGCTACAAA CGAAGACCAC AGGCAAATCC TAAGAGAGAA CAATTGCCTA 
CAAACTTTAT TACAGCACTT GAAATCTCAC AGCTTGACAA TAGTCAGTAA TGCATGTGGA 
ACTTTGTGGA ATCTCTCAGC AAGAAATCCT AAAGACCAGG AAGCCTTGTG GGACATGGGG 
GCAGTGAGCA TGCTCAAGAA CCTCATTCAT TCCAAGCACA AAATGATTGC CATGGGAAGT 
GCAGCAGCTT TAAGGAATCT CATGGCAAAC AGACCTGCAA AGTATAAGGA TGCCAATATC 
ATGTCTCCCG GCTCAAGTCT GCCATCCCTT CACGTTAGGA AACAGAAAGC TCTAGAAGCT 
GAGCTAGATG CTCAGCATTT ATCAGAAACC TTCGACAACA TTGACAACCT AAGTCCCAAG 
GCCTCTCACC GGAGTAAGCA GAGACACAAG CAGAATCTTT ATGGTGACTA TGCTTTTGAC 
GCCAATCGAC ATGATGATAG TAGGTCAGAC AATTTCAATA CTGGAAACAT GACTGTTCTT 
TCACCATATT TAAATACTAC GGTATTGCCC AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC 
AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG GAGAGAGAGC GAGGTATTGG CCTCAGTGCT 
TACCATCCAA CAACAGAAAA TGCAGGAACC TCATCAAAAC GAGGTCTGCA GATCACTACC 
ACTGCAGCCC AGATAGCCAA AGTTATGGAA GAAGTATCAG CCATTCATAC CTCCCAGGAC 
GACAGAAGTT CTGCTTCTAC CACCGAGTTC CATTGTGTGG CAGACGACAG GAGTGCGGCA 
CGAAGAAGCT CTGCCTCCCA CACACACTCA AACACATACA ACTTCACTAA GTCGGAAAAT 
TCAAATAGGA CATGCTCTAT GCCTTATGCC AAAGTGGAAT ATAAACGATC TTCAAATGAC 
AGTTTAAATA GTGTCACTAG TAGTGATGGA TATGGTAAAA GAGGCCAAAT GAAACCCTCA 
GTTGAATCCT ATTCTGAAGA TGATGAAAGT AAATTTTGCA GTTATGGTCA GTATCCAGCT 
GACCTAGCCC ATAAGATACA CAGTGCAAAT CATATGGATG ATAATGATGG AGAACTGGAT 
ACACCAATAA ATTACAGTCT TAAATATTCA GATGAGCAGT TGAACTCAGG AAGGCAGAGT 
CCCTCACAGA ATGAAAGGTG GGCAAGACCA AAGCATGTGA TAGAAGATGA AATAAAGCAA 
AACGAGCAAA GACAAGCAAG AAGCCAGAAC ACCAGTTATC CTGTCTATTC TGAGAATACC 
GATGACAAAC ACCTCAAATT CCAACCACAT TTTGGACAAC AAGAATGTGT TTCCCCATAT 
AGGTCAAGGG GAACCAGTGG TTCAGAAACA AATCGAATGG GTTCTAGTCA TGCAATTAAT 
CAAAATGTAA ACCAGTCTCT GTGTCAGGAA GATGATTATG AAGATGATAA ACCTACCAAC 
TACAGTGAAC GTTATTCTGA GGAAGAACAA CATGAAGAAG AAGAAGAGAG ACCGACAAAT 
TATAGCATAA AATATAATGA AGAGAAACAT CATGTGGATC AGCCTATTGA TTATAGTTTA 
AAATATGCCA CTGACATTTC TTCCTCACAA AAACCATCAT TTTCATTCTC AAAGAATTCA 
TCAGCACAAA GCACTAAACC TGAACATCTC TCTCCAAGCA GCGAGAATAC AGCTGTACCT 
CCATCTAATG CCAAAAGGCA GAATCAGCTG CGTCCAAGTT CAGCACAAAG AAATGGCCAG 
ACTCAAAAAG GCACTACTTG CAAAGTCCCC TCCATCAACC AAGAAACAAT ACAGACTTAC 
TGCGTAGAAG ACACCCCAAT ATGTTTTTCA AGGTGCAGTT CATTATCATC ACTGTCATCA 
GCTGACGATG AAATAGGATG TGATCAGACA ACACAGGAAG CAGATTCTGC TAATACTCTG 
CAGACAGCAG AAGTAAAAGA GAATGATGTA ACTCGGTCAG CTGAAGATCC TGCAACTGAA 
GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC AAACCCAGCC GACTCCAGGC TTCTGGCTTA 
TCTTCAGAAT CAACCAGGCA TAATAAAGCT GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC 
TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT CCCCCAGAAC ACTATGTCCA GGAGACTCCG 
CTCGTATTCA GCAGGTGTAC TTCTGTCAGC TCCCTTGACA GTTTTGAGAG TCGCTCCATT 
GCCAGCTCTG TTCAGAGTGA GCCATGTAGT GGAATGGTGA GTGGCATCAT AAGCCCCAGT 
GACCTTCCAG ATAGTCCTGG GCAGACCATG CCACCAAGCA GAAGCAAAAC CCCTCCACCT 
CCTCCACAGA CAGTGCAGGC CAAGAGAGAG GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG 
AAGAGAGAGA GTGGGCCTAA GCAGACTGCT GTAAATGCTG CCGTGCAGAG GGTGCAGGTC 
CTTCCAGACG TGGATACTTT GTTACACTTC GCCACAGAAA GTACTCCAGA CGGGTTTTCT 
TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG GATGAGCCAT TTATACAGAA AGATGTAGAA 
TTAAGAATCA TGCCTCCAGT TCAGGAAAAC GACAATGGGA ATGAAACTGA ATCAGAACAG 
CCTGAGGAAT CAAATGAAAA CCAGGATAAA GAGGTAGAAA AGCCTGACTC TGAAAAAGAC 
TTATTAGATG ATTCTGATGA CGATGATATT GAAATATTAG AAGAATGTAT TATTTCAGCC 
ATGCCAACAA AGTCATCACG CAAAGCCAAA AAACTAGCCC AGACTGCTTC AAAATTACCT 
CCACCTGTGG CAAGGAAACC AAGTCAGCTA CCTGTGTATA AACTTCTGCC AGCACAGAAT 
AGGCTGCAGG CACAAAAACA TGTTAGCTTT ACACCAGGGG ATGATGTGCC CCGGGTGTAC 
TGTGTAGAAG GGACACCTAT AAACTTTTCC ACAGCAACGT CTCTAAGTGA TCTGACAATA 
GAGTCCCCTC CAAATGAATT GGCTACTGGA GATGGGGTCA GAGCGGGTAT ACAGTCAGGT 
GAATTTGAAA AACGAGATAC CATTCCTACA GAAGGCAGAA GTACAGATGA TGCTCAGCGA 
GGAAAAATCT CATCTATAGT TACACCAGAC CTGGATGACA ACAAAGCAGA GGAAGGAGAT 
ATTCTTGCAG AATGTATCAA TTCTGCTATG CCCAAAGGAA AAAGCCACAA GCCTTTCCGA 
GTGAAAAAGA TAATGGACCA AGTCCAACAA GCATCCTCGA CTTCATCTGG AGCTAACAAA 
AATCAAGTAG ACACTAAGAA AAAGAAGCCT ACTTCACCAG TAAAGCCCAT GCCACAAAAT 
ACTGAATATA GAACGCGTGT GAGAAAGAAT ACAGACTCAA AAGTTAATGT AAATACTGAA 
GAAACTTTCT CAGACAACAA AGACTCAAAG AAACCAAGCT TACAAACCAA TGCCAAGGCC 
TTCAATGAAA AGCTACCTAA CAATGAAGAC AGAGTGCGGG GGAGCTTCGC CTTGGACTCA 
CCGCATCACT ACACCCCTAT TGAGGGGACG CCGTACTGCT TTTCCCGAAA TGACTCCTTG 
AGTTCTCTGG ATTTTGATGA TGACGATGTT GACCTTTCCA GGGAAAAGGC CGAGTTAAGA 
AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC AAAGTTACCT GCCGCCCAGA ACCAAACTCA 
AGCCAGCAGG CAGCTAGTAA GTCACAAGCC AGTATAAAAC ATCCAGCAAA CAGAGCACAG 
TCCAAACCAG TGCTGCAGAA ACAGCCCACT TTCCCCCAGT CCTCCAAAGA CGGACCAGAT 
AGAGGGGCAG CAACTGACGA AAAACTGCAG AATTTTGCTA TTGAAAATAC TCCAGTTTGC 
TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT AGTGACATTG ACCAGGAAAA CAACAATAAC 
AAAGAAAGTG AACCAATCAA AGAAGCTGAA CCTGCCAACT CACAAGGAGA GCCCAGTAAG 
CCTCAGGCAT CCGGGTATGC TCCCAAGTCC TTCCACGTCG AAGACACCCC TGTCTGTTTC 
TCAAGAAACA GCTCTCTCAG TTCTCTTAGC ATTGACTCTG AGGACGACCT GTTACAGGAG 
TGTATAAGTT CTGCCATGCC AAAAAAGAAA AGGCCTTCAA GACTCAAGAG TGAGAGCGAA 
AAGCAGAGCC CTAGAAAAGT GGGTGGCATA TTAGCTGAAG ACCTGACGCT TGATTTGAAA 
GATCTACAGA GGCCAGATTC AGAACACGCT TTCTCCCCCG ACTCAGAAAA TTTTGACTGG 
AAAGCTATTC AGGAAGGCGC AAACTCCATA GTAAGTAGTT TGCACCAAGC TGCTGCAGCC 
GCCGCGTGCT TATCTAGACA AGCGTCATCC GACTCAGATT CCATTCTGTC ACTAAAGTCC 
GGCATTTCTC TGGGATCGCC TTTTCATCTT ACACCTGATC AAGAGGAAAA GCCATTCACA 
AGCAATAAAG GCCCAAGAAT TCTCAAACCT GGAGAGAAAA GCACATTAGA AGCAAAAAAA 
ATAGAATCTG AAAACAAAGG AATCAAAGGC GGGAAAAAGG TTTATAAAAG CTTGATTACG 
GGAAAGATTC GCTCCAATTC AGAAATTTCC AGCCAAATGA AACAACCCCT CCCGACAAAC 
ATGCCTTCAA TCTCAAGAGG CAGGACGATG ATTCACATCC CAGGGCTTCG GAATAGCTCC 
TCTAGTACAA GCCCTGTCTC TAAGAAAGGC CCACCCCTCA AGACTCCAGC CTCTAAAAGC 
CCCAGTGAAG GGCCGGGAGC TACCACTTCT CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA 
GAGCTTAGCC CTATCACCAG GCAAACTTCC CAAATCAGTG GGTCAAATAA GGGGTCTTCT 
AGATCAGGAT CTAGAGACTC CACTCCCTCA AGACCTACAC AGCAACCATT AAGTAGGCCA 
ATGCAGTCTC CAGGGCGAAA CTCAATTTCC CCTGGTAGAA ATGGAATAAG CCCTCCTAAC 
AAACTGTCTC AGCTGCCCAG AACATCATCT CCCAGTACTG CTTCAACTAA GTCCTCCGGT 
TCTGGGAAAA TGTCATATAC ATCCCCAGGT AGACAGCTGA GCCAACAAAA TCTTACCAAA 
CAAGCAAGTT TATCCAAGAA TGCCAGCAGT ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA 
CTGAATCAGA TGAGTAACGG CAATGGGTCA AATAAAAAGG TAGAACTTTC TAGAATGTCT 
TCAACTAAAT CAAGTGGAAG TGAATCAGAC AGATCAGAAA GGCCTGCATT AGTACGCCAG 
TCTACTTTCA TCAAAGAAGC CCCAAGCCCA ACCCTGAGGA GGAAACTGGA GGAATCTGCC 
TCATTTGAAT CCCTTTCTCC ATCTTCTAGA CCAGATTCTC CCACCAGGTC GCAGGCACAG 
ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT ATGTCTCTGT CCACACATCC ATCTGTTCAG 
GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT CTCAGCCCCA CTATCGAGTA TAATGACGGA 
AGGCCCACAA AACGGCATGA TATTGCACGC TCCCATTCTG AAAGTCCTTC CAGACTACCA 
ATCAACCGGG CGGGAACCTG GAAGCGTGAA CACAGCAAAC ATTCCTCGTC CCTTCCTCGA 
GTGAGTACTT GGAGAAGAAC TGGAAGCTCA TCTTCTATTC TTTCTGCTTC ATCAGAGTCC 
AGTGAAAAAG CAAAAAGTGA GGATGAAAGG CATGTGAGCT CCATGCCAGC ACCCAGACAG 
ATGAAGGAAA ACCAGGTGCC CACCAAAGGA ACATGGAGGA AAATCAAGGA AAGTGACATT 
TCTCCCACAG GCATGGCTTC TCAGAGCGCT TCCTCAGGTG CTGCCAGTGG TGCTGAATCC 
AAGCCTCTGA TCTATCAGAT GGCACCTCCT GTCTCTAAAA CAGAGGATGT TTGGGTGAGA 
ATTGAGGACT GCCCCATTAA CAACCCTAGA TCTGGACGGT CCCCCACAGG CAACACCCCC 
CCAGTGATTG ACAGTGTTTC AGAGAAGGGA AGTTCAAGCA TTAAAGATTC AAAAGACACC 
CATGGGAAAC AGAGTGTGGG CAGTGGCAGT CCTGTGCAAA CCGTGGGTCT GGAAACCCGC 
CTCAACTCCT TTGTTCAGGT AGAGGCCCCA GAACAGAAAG GAACTGAGGC AAAACCAGGA 
CAGAGTAACC CAGTCTCTAT AGCAGAGACT GCTGAGACGT GTATAGCAGA GCGTACCCCT 
TTCAGTTCCA GTAGCTCCAG CAAGCACAGC TCACCTAGCG GGACTGTTGC TGCCAGAGTG 
ACACCTTTTA ATTACAACCC TAGCCCTAGG AAGAGCAGCG CAGACAGCAC TTCAGCCCGG 
CCGTCTCAGA TCCCTACGCC AGTGAGCACC AACACGAAGA AGAGAGATTC GAAGACTGAC 
AGCACAGAAT CCAGTGGAGC CCAAAGTCCT AAACGCCATT CCGGGTCTTA CCTCGTGACG 
TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106156.1
CDS	294..8342
Translation	MTSGQIDLLERLKEFNLDSNFPGVKLRSKMSLRSYGSREGSVSSRSGECSPVPMGSFPRRTFVNGSRESTGYLEELEKERSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQRRIARIQQIEKDILRVRQLLQSQAAEAERSSQSRHDAASHEAGRQHEGHGVAESNTAASSSGQSPATRVDHETASVLSSSGTHSAPRRLTSHLGTKIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCVLMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGDVANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKALMECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAIIESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGTSSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHSNTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHVIEDEIKQNEQRQARSQNTSYPVYSENTDDKHLKFQPHFGQQECVSPYRSRGTSGSETNRMGSSHAINQNVNQSLCQEDDYEDDKPTNYSERYSEEEQHEEEEERPTNYSIKYNEEKHHVDQPIDYSLKYATDISSSQKPSFSFSKNSSAQSTKPEHLSPSSENTAVPPSNAKRQNQLRPSSAQRNGQTQKGTTCKVPSINQETIQTYCVEDTPICFSRCSSLSSLSSADDEIGCDQTTQEADSANTLQTAEVKENDVTRSAEDPATEVPAVSQNARAKPSRLQASGLSSESTRHNKAVEFSSGAKSPSKSGAQTPKSPPEHYVQETPLVFSRCTSVSSLDSFESRSIASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSRSKTPPPPPQTVQAKREVPKSKVPAAEKRESGPKQTAVNAAVQRVQVLPDVDTLLHFATESTPDGFSCSSSLSALSLDEPFIQKDVELRIMPPVQENDNGNETESEQPEESNENQDKEVEKPDSEKDLLDDSDDDDIEILEECIISAMPTKSSRKAKKLAQTASKLPPPVARKPSQLPVYKLLPAQNRLQAQKHVSFTPGDDVPRVYCVEGTPINFSTATSLSDLTIESPPNELATGDGVRAGIQSGEFEKRDTIPTEGRSTDDAQRGKISSIVTPDLDDNKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQVQQASSTSSGANKNQVDTKKKKPTSPVKPMPQNTEYRTRVRKNTDSKVNVNTEETFSDNKDSKKPSLQTNAKAFNEKLPNNEDRVRGSFALDSPHHYTPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCRPEPNSSQQAASKSQASIKHPANRAQSKPVLQKQPTFPQSSKDGPDRGAATDEKLQNFAIENTPVCFSRNSSLSSLSDIDQENNNNKESEPIKEAEPANSQGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSLSIDSEDDLLQECISSAMPKKKRPSRLKSESEKQSPRKVGGILAEDLTLDLKDLQRPDSEHAFSPDSENFDWKAIQEGANSIVSSLHQAAAAAACLSRQASSDSDSILSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKPGEKSTLEAKKIESENKGIKGGKKVYKSLITGKIRSNSEISSQMKQPLPTNMPSISRGRTMIHIPGLRNSSSSTSPVSKKGPPLKTPASKSPSEGPGATTSPRGTKPAGKSELSPITRQTSQISGSNKGSSRSGSRDSTPSRPTQQPLSRPMQSPGRNSISPGRNGISPPNKLSQLPRTSSPSTASTKSSGSGKMSYTSPGRQLSQQNLTKQASLSKNASSIPRSESASKGLNQMSNGNGSNKKVELSRMSSTKSSGSESDRSERPALVRQSTFIKEAPSPTLRRKLEESASFESLSPSSRPDSPTRSQAQTPVLSPSLPDMSLSTHPSVQAGGWRKLPPNLSPTIEYNDGRPTKRHDIARSHSESPSRLPINRAGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDERHVSSMPAPRQMKENQVPTKGTWRKIKESDISPTGMASQSASSGAASGAESKPLIYQMAPPVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKGSSSIKDSKDTHGKQSVGSGSPVQTVGLETRLNSFVQVEAPEQKGTEAKPGQSNPVSIAETAETCIAERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVSTNTKKRDSKTDSTESSGAQSPKRHSGSYLVTSV

Target ORF information:

RefSeq Version	XM_030250296.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus APC, WNT signaling pathway regulator (Apc), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250296.1

ORF Insert Sequence:

ATGACTTCTG GACAGATTGA CTTACTAGAG CGTCTTAAAG AATTTAACTT AGATAGTAAT 
TTCCCCGGAG TGAAACTACG CTCAAAAATG TCCCTTCGCT CCTACGGAAG TCGGGAAGGA 
TCTGTATCCA GCCGTTCAGG AGAATGCAGT CCTGTCCCCA TGGGGTCATT CCCAAGAAGA 
ACATTTGTAA ATGGAAGCAG AGAGAGTACT GGGTATCTAG AAGAGCTTGA AAAAGAAAGA 
TCATTACTCC TTGCTGATCT TGACAAAGAA GAGAAGGAAA AGGACTGGTA TTATGCTCAA 
CTTCAGAACC TCACAAAAAG AATAGATAGC CTGCCTTTAA CTGAAAATTT TTCCTTACAG 
ACAGACATGA CAAGACGGCA GCTGGAGTAT GAAGCAAGGC AGATCAGGGC TGCAATGGAG 
GAGCAGCTTG GCACCTGCCA GGACATGGAG AAGCGTGCAC AGCGAAGAAT AGCCAGGATC 
CAGCAAATAG AAAAGGACAT ACTGCGCGTG CGCCAGCTTT TACAGTCCCA GGCGGCGGAA 
GCGGAGAGGT CATCTCAGAG CAGGCATGAT GCTGCCTCCC ATGAAGCTGG CCGGCAGCAC 
GAAGGCCACG GAGTGGCAGA AAGCAACACC GCAGCCTCCA GTAGTGGTCA GAGTCCAGCT 
ACACGTGTGG ATCACGAAAC AGCCAGTGTT TTGAGTTCTA GCGGCACGCA CTCTGCTCCT 
CGAAGGTTGA CAAGTCATCT GGGGACAAAG ATACGAGCTT ACTGTGAAAC CTGTTGGGAG 
TGGCAGGAAG CCCACGAACA AGGCATGGAC CAGGACAAAA ACCCAATGCC AGCTCCTGTT 
GAGCATCAGA TCTGTCCTGC TGTGTGTGTT CTAATGAAGC TTTCATTTGA TGAAGAGCAT 
AGGCATGCAA TGAATGAACT TGGGGGACTG CAGGCCATTG CAGAGTTATT GCAGGTGGAC 
TGTGAGATGT ATGGGCTTAC TAATGACCAC TACAGTGTTA CTTTAAGACG GTATGCTGGA 
ATGGCTTTGA CAAACTTGAC CTTTGGAGAT GTTGCCAACA AGGCTACGCT GTGTTCTATG 
AAAGGCTGCA TGAGAGCACT TGTGGCCCAG TTAAAATCTG AGAGTGAAGA CTTACAGCAG 
GTTATTGCAA GTGTTTTGAG GAATTTGTCT TGGCGAGCAG ATGTAAATAG CAAAAAGACG 
TTGAGAGAAG TTGGAAGTGT GAAAGCATTG ATGGAATGTG CTTTGGAAGT TAAAAAGGAA 
TCAACCCTCA AAAGCGTTTT GAGTGCCTTA TGGAACCTGT CTGCACACTG CACTGAGAAT 
AAGGCTGACA TCTGTGCTGT GGATGGAGCA CTGGCATTTC TGGTTGGCAC CCTCACTTAC 
CGGAGCCAGA CAAATACTTT AGCCATTATT GAAAGTGGAG GTGGGATATT ACGGAATGTG 
TCCAGCTTGA TAGCTACAAA CGAAGACCAC AGGCAAATCC TAAGAGAGAA CAATTGCCTA 
CAAACTTTAT TACAGCACTT GAAATCTCAC AGCTTGACAA TAGTCAGTAA TGCATGTGGA 
ACTTTGTGGA ATCTCTCAGC AAGAAATCCT AAAGACCAGG AAGCCTTGTG GGACATGGGG 
GCAGTGAGCA TGCTCAAGAA CCTCATTCAT TCCAAGCACA AAATGATTGC CATGGGAAGT 
GCAGCAGCTT TAAGGAATCT CATGGCAAAC AGACCTGCAA AGTATAAGGA TGCCAATATC 
ATGTCTCCCG GCTCAAGTCT GCCATCCCTT CACGTTAGGA AACAGAAAGC TCTAGAAGCT 
GAGCTAGATG CTCAGCATTT ATCAGAAACC TTCGACAACA TTGACAACCT AAGTCCCAAG 
GCCTCTCACC GGAGTAAGCA GAGACACAAG CAGAATCTTT ATGGTGACTA TGCTTTTGAC 
GCCAATCGAC ATGATGATAG TAGGTCAGAC AATTTCAATA CTGGAAACAT GACTGTTCTT 
TCACCATATT TAAATACTAC GGTATTGCCC AGCTCTTCTT CCTCAAGGGG AAGTTTAGAC 
AGTTCTCGTT CTGAGAAAGA CAGAAGTTTG GAGAGAGAGC GAGGTATTGG CCTCAGTGCT 
TACCATCCAA CAACAGAAAA TGCAGGAACC TCATCAAAAC GAGGTCTGCA GATCACTACC 
ACTGCAGCCC AGATAGCCAA AGTTATGGAA GAAGTATCAG CCATTCATAC CTCCCAGGAC 
GACAGAAGTT CTGCTTCTAC CACCGAGTTC CATTGTGTGG CAGACGACAG GAGTGCGGCA 
CGAAGAAGCT CTGCCTCCCA CACACACTCA AACACATACA ACTTCACTAA GTCGGAAAAT 
TCAAATAGGA CATGCTCTAT GCCTTATGCC AAAGTGGAAT ATAAACGATC TTCAAATGAC 
AGTTTAAATA GTGTCACTAG TAGTGATGGA TATGGTAAAA GAGGCCAAAT GAAACCCTCA 
GTTGAATCCT ATTCTGAAGA TGATGAAAGT AAATTTTGCA GTTATGGTCA GTATCCAGCT 
GACCTAGCCC ATAAGATACA CAGTGCAAAT CATATGGATG ATAATGATGG AGAACTGGAT 
ACACCAATAA ATTACAGTCT TAAATATTCA GATGAGCAGT TGAACTCAGG AAGGCAGAGT 
CCCTCACAGA ATGAAAGGTG GGCAAGACCA AAGCATGTGA TAGAAGATGA AATAAAGCAA 
AACGAGCAAA GACAAGCAAG AAGCCAGAAC ACCAGTTATC CTGTCTATTC TGAGAATACC 
GATGACAAAC ACCTCAAATT CCAACCACAT TTTGGACAAC AAGAATGTGT TTCCCCATAT 
AGGTCAAGGG GAACCAGTGG TTCAGAAACA AATCGAATGG GTTCTAGTCA TGCAATTAAT 
CAAAATGTAA ACCAGTCTCT GTGTCAGGAA GATGATTATG AAGATGATAA ACCTACCAAC 
TACAGTGAAC GTTATTCTGA GGAAGAACAA CATGAAGAAG AAGAAGAGAG ACCGACAAAT 
TATAGCATAA AATATAATGA AGAGAAACAT CATGTGGATC AGCCTATTGA TTATAGTTTA 
AAATATGCCA CTGACATTTC TTCCTCACAA AAACCATCAT TTTCATTCTC AAAGAATTCA 
TCAGCACAAA GCACTAAACC TGAACATCTC TCTCCAAGCA GCGAGAATAC AGCTGTACCT 
CCATCTAATG CCAAAAGGCA GAATCAGCTG CGTCCAAGTT CAGCACAAAG AAATGGCCAG 
ACTCAAAAAG GCACTACTTG CAAAGTCCCC TCCATCAACC AAGAAACAAT ACAGACTTAC 
TGCGTAGAAG ACACCCCAAT ATGTTTTTCA AGGTGCAGTT CATTATCATC ACTGTCATCA 
GCTGACGATG AAATAGGATG TGATCAGACA ACACAGGAAG CAGATTCTGC TAATACTCTG 
CAGACAGCAG AAGTAAAAGA GAATGATGTA ACTCGGTCAG CTGAAGATCC TGCAACTGAA 
GTTCCAGCAG TGTCCCAGAA TGCTAGAGCC AAACCCAGCC GACTCCAGGC TTCTGGCTTA 
TCTTCAGAAT CAACCAGGCA TAATAAAGCT GTTGAGTTTT CTTCAGGAGC CAAGTCTCCC 
TCCAAAAGTG GTGCTCAGAC ACCCAAAAGT CCCCCAGAAC ACTATGTCCA GGAGACTCCG 
CTCGTATTCA GCAGGTGTAC TTCTGTCAGC TCCCTTGACA GTTTTGAGAG TCGCTCCATT 
GCCAGCTCTG TTCAGAGTGA GCCATGTAGT GGAATGGTGA GTGGCATCAT AAGCCCCAGT 
GACCTTCCAG ATAGTCCTGG GCAGACCATG CCACCAAGCA GAAGCAAAAC CCCTCCACCT 
CCTCCACAGA CAGTGCAGGC CAAGAGAGAG GTGCCAAAAA GTAAAGTCCC TGCTGCTGAG 
AAGAGAGAGA GTGGGCCTAA GCAGACTGCT GTAAATGCTG CCGTGCAGAG GGTGCAGGTC 
CTTCCAGACG TGGATACTTT GTTACACTTC GCCACAGAAA GTACTCCAGA CGGGTTTTCT 
TGTTCCTCCA GCCTAAGTGC TCTGAGCCTG GATGAGCCAT TTATACAGAA AGATGTAGAA 
TTAAGAATCA TGCCTCCAGT TCAGGAAAAC GACAATGGGA ATGAAACTGA ATCAGAACAG 
CCTGAGGAAT CAAATGAAAA CCAGGATAAA GAGGTAGAAA AGCCTGACTC TGAAAAAGAC 
TTATTAGATG ATTCTGATGA CGATGATATT GAAATATTAG AAGAATGTAT TATTTCAGCC 
ATGCCAACAA AGTCATCACG CAAAGCCAAA AAACTAGCCC AGACTGCTTC AAAATTACCT 
CCACCTGTGG CAAGGAAACC AAGTCAGCTA CCTGTGTATA AACTTCTGCC AGCACAGAAT 
AGGCTGCAGG CACAAAAACA TGTTAGCTTT ACACCAGGGG ATGATGTGCC CCGGGTGTAC 
TGTGTAGAAG GGACACCTAT AAACTTTTCC ACAGCAACGT CTCTAAGTGA TCTGACAATA 
GAGTCCCCTC CAAATGAATT GGCTACTGGA GATGGGGTCA GAGCGGGTAT ACAGTCAGGT 
GAATTTGAAA AACGAGATAC CATTCCTACA GAAGGCAGAA GTACAGATGA TGCTCAGCGA 
GGAAAAATCT CATCTATAGT TACACCAGAC CTGGATGACA ACAAAGCAGA GGAAGGAGAT 
ATTCTTGCAG AATGTATCAA TTCTGCTATG CCCAAAGGAA AAAGCCACAA GCCTTTCCGA 
GTGAAAAAGA TAATGGACCA AGTCCAACAA GCATCCTCGA CTTCATCTGG AGCTAACAAA 
AATCAAGTAG ACACTAAGAA AAAGAAGCCT ACTTCACCAG TAAAGCCCAT GCCACAAAAT 
ACTGAATATA GAACGCGTGT GAGAAAGAAT ACAGACTCAA AAGTTAATGT AAATACTGAA 
GAAACTTTCT CAGACAACAA AGACTCAAAG AAACCAAGCT TACAAACCAA TGCCAAGGCC 
TTCAATGAAA AGCTACCTAA CAATGAAGAC AGAGTGCGGG GGAGCTTCGC CTTGGACTCA 
CCGCATCACT ACACCCCTAT TGAGGGGACG CCGTACTGCT TTTCCCGAAA TGACTCCTTG 
AGTTCTCTGG ATTTTGATGA TGACGATGTT GACCTTTCCA GGGAAAAGGC CGAGTTAAGA 
AAGGGCAAAG AAAGCAAGGA TTCCGAAGCC AAAGTTACCT GCCGCCCAGA ACCAAACTCA 
AGCCAGCAGG CAGCTAGTAA GTCACAAGCC AGTATAAAAC ATCCAGCAAA CAGAGCACAG 
TCCAAACCAG TGCTGCAGAA ACAGCCCACT TTCCCCCAGT CCTCCAAAGA CGGACCAGAT 
AGAGGGGCAG CAACTGACGA AAAACTGCAG AATTTTGCTA TTGAAAATAC TCCAGTTTGC 
TTTTCTCGAA ATTCCTCTCT GAGTTCCCTT AGTGACATTG ACCAGGAAAA CAACAATAAC 
AAAGAAAGTG AACCAATCAA AGAAGCTGAA CCTGCCAACT CACAAGGAGA GCCCAGTAAG 
CCTCAGGCAT CCGGGTATGC TCCCAAGTCC TTCCACGTCG AAGACACCCC TGTCTGTTTC 
TCAAGAAACA GCTCTCTCAG TTCTCTTAGC ATTGACTCTG AGGACGACCT GTTACAGGAG 
TGTATAAGTT CTGCCATGCC AAAAAAGAAA AGGCCTTCAA GACTCAAGAG TGAGAGCGAA 
AAGCAGAGCC CTAGAAAAGT GGGTGGCATA TTAGCTGAAG ACCTGACGCT TGATTTGAAA 
GATCTACAGA GGCCAGATTC AGAACACGCT TTCTCCCCCG ACTCAGAAAA TTTTGACTGG 
AAAGCTATTC AGGAAGGCGC AAACTCCATA GTAAGTAGTT TGCACCAAGC TGCTGCAGCC 
GCCGCGTGCT TATCTAGACA AGCGTCATCC GACTCAGATT CCATTCTGTC ACTAAAGTCC 
GGCATTTCTC TGGGATCGCC TTTTCATCTT ACACCTGATC AAGAGGAAAA GCCATTCACA 
AGCAATAAAG GCCCAAGAAT TCTCAAACCT GGAGAGAAAA GCACATTAGA AGCAAAAAAA 
ATAGAATCTG AAAACAAAGG AATCAAAGGC GGGAAAAAGG TTTATAAAAG CTTGATTACG 
GGAAAGATTC GCTCCAATTC AGAAATTTCC AGCCAAATGA AACAACCCCT CCCGACAAAC 
ATGCCTTCAA TCTCAAGAGG CAGGACGATG ATTCACATCC CAGGGCTTCG GAATAGCTCC 
TCTAGTACAA GCCCTGTCTC TAAGAAAGGC CCACCCCTCA AGACTCCAGC CTCTAAAAGC 
CCCAGTGAAG GGCCGGGAGC TACCACTTCT CCTCGAGGAA CTAAGCCAGC AGGAAAGTCA 
GAGCTTAGCC CTATCACCAG GCAAACTTCC CAAATCAGTG GGTCAAATAA GGGGTCTTCT 
AGATCAGGAT CTAGAGACTC CACTCCCTCA AGACCTACAC AGCAACCATT AAGTAGGCCA 
ATGCAGTCTC CAGGGCGAAA CTCAATTTCC CCTGGTAGAA ATGGAATAAG CCCTCCTAAC 
AAACTGTCTC AGCTGCCCAG AACATCATCT CCCAGTACTG CTTCAACTAA GTCCTCCGGT 
TCTGGGAAAA TGTCATATAC ATCCCCAGGT AGACAGCTGA GCCAACAAAA TCTTACCAAA 
CAAGCAAGTT TATCCAAGAA TGCCAGCAGT ATCCCCAGAA GTGAGTCGGC ATCTAAAGGA 
CTGAATCAGA TGAGTAACGG CAATGGGTCA AATAAAAAGG TAGAACTTTC TAGAATGTCT 
TCAACTAAAT CAAGTGGAAG TGAATCAGAC AGATCAGAAA GGCCTGCATT AGTACGCCAG 
TCTACTTTCA TCAAAGAAGC CCCAAGCCCA ACCCTGAGGA GGAAACTGGA GGAATCTGCC 
TCATTTGAAT CCCTTTCTCC ATCTTCTAGA CCAGATTCTC CCACCAGGTC GCAGGCACAG 
ACCCCAGTTT TAAGCCCTTC CCTTCCTGAT ATGTCTCTGT CCACACATCC ATCTGTTCAG 
GCAGGTGGGT GGCGAAAGCT CCCGCCTAAT CTCAGCCCCA CTATCGAGTA TAATGACGGA 
AGGCCCACAA AACGGCATGA TATTGCACGC TCCCATTCTG AAAGTCCTTC CAGACTACCA 
ATCAACCGGG CGGGAACCTG GAAGCGTGAA CACAGCAAAC ATTCCTCGTC CCTTCCTCGA 
GTGAGTACTT GGAGAAGAAC TGGAAGCTCA TCTTCTATTC TTTCTGCTTC ATCAGAGTCC 
AGTGAAAAAG CAAAAAGTGA GGATGAAAGG CATGTGAGCT CCATGCCAGC ACCCAGACAG 
ATGAAGGAAA ACCAGGTGCC CACCAAAGGA ACATGGAGGA AAATCAAGGA AAGTGACATT 
TCTCCCACAG GCATGGCTTC TCAGAGCGCT TCCTCAGGTG CTGCCAGTGG TGCTGAATCC 
AAGCCTCTGA TCTATCAGAT GGCACCTCCT GTCTCTAAAA CAGAGGATGT TTGGGTGAGA 
ATTGAGGACT GCCCCATTAA CAACCCTAGA TCTGGACGGT CCCCCACAGG CAACACCCCC 
CCAGTGATTG ACAGTGTTTC AGAGAAGGGA AGTTCAAGCA TTAAAGATTC AAAAGACACC 
CATGGGAAAC AGAGTGTGGG CAGTGGCAGT CCTGTGCAAA CCGTGGGTCT GGAAACCCGC 
CTCAACTCCT TTGTTCAGGT AGAGGCCCCA GAACAGAAAG GAACTGAGGC AAAACCAGGA 
CAGAGTAACC CAGTCTCTAT AGCAGAGACT GCTGAGACGT GTATAGCAGA GCGTACCCCT 
TTCAGTTCCA GTAGCTCCAG CAAGCACAGC TCACCTAGCG GGACTGTTGC TGCCAGAGTG 
ACACCTTTTA ATTACAACCC TAGCCCTAGG AAGAGCAGCG CAGACAGCAC TTCAGCCCGG 
CCGTCTCAGA TCCCTACGCC AGTGAGCACC AACACGAAGA AGAGAGATTC GAAGACTGAC 
AGCACAGAAT CCAGTGGAGC CCAAAGTCCT AAACGCCATT CCGGGTCTTA CCTCGTGACG 
TCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Editing of the gut microbiota reduces carcinogenesis in mouse models of colitis-associated colorectal cancer.
The Journal of experimental medicine216(10)2378-2393(2019 Oct)
Zhu W,Miyata N,Winter MG,Arenales A,Hughes ER,Spiga L,Kim J,Sifuentes-Dominguez L,Starokadomskyy P,Gopal P,Byndloss MX,Santos RL,Burstein E,Winter SE

Epithelial NOTCH Signaling Rewires the Tumor Microenvironment of Colorectal Cancer to Drive Poor-Prognosis Subtypes and Metastasis.
Cancer cell36(3)319-336.e7(2019 Sep)
Jackstadt R,van Hooff SR,Leach JD,Cortes-Lavaud X,Lohuis JO,Ridgway RA,Wouters VM,Roper J,Kendall TJ,Roxburgh CS,Horgan PG,Nixon C,Nourse C,Gunzer M,Clark W,Hedley A,Yilmaz OH,Rashid M,Bailey P,Biankin AV,Campbell AD,Adams DJ,Barry ST,Steele CW,Medema JP,Sansom OJ

TRIM67 Activates p53 to Suppress Colorectal Cancer Initiation and Progression.
Cancer research79(16)4086-4098(2019 Aug)
Wang S,Zhang Y,Huang J,Wong CC,Zhai J,Li C,Wei G,Zhao L,Wang G,Wei H,Zhao Z,Yu J

Determination of the Potential Tumor-Suppressive Effects of
Cancers11(8)(2019 Aug)
Croes L,Fransen E,Hylebos M,Buys K,Hermans C,Broeckx G,Peeters M,Pauwels P,Op de Beeck K,Van Camp G

Lineage tracing and targeting of IL17RB
Proceedings of the National Academy of Sciences of the United States of America116(26)12996-13005(2019 Jun)
Goto N,Fukuda A,Yamaga Y,Yoshikawa T,Maruno T,Maekawa H,Inamoto S,Kawada K,Sakai Y,Miyoshi H,Taketo MM,Chiba T,Seno H

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EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

Apc cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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