Ap1g1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ap1g1
Entrez Gene ID	11765
Full Name	adaptor protein complex AP-1, gamma 1 subunit
Synonyms	AA409002,AU041323,AW551707,Adtg,D8Ertd374e
General protein information	Preferred Names adaptor protein complex AP-1, gamma 1 subunit Names AP-1 complex subunit gamma-1 adapter-related protein complex 1 subunit gamma-1 adaptor protein complex AP-1 subunit gamma-1 adaptor-related protein complex 1 subunit gamma-1 clathrin assembly protein complex 1 gamma-1 large chain gamma-adaptin gamma1-adaptin golgi adaptor HA1/AP1 adaptin subunit gamma-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 D3\|8 57.26 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001301211.1	NP_001288140.1	AP-1 complex subunit gamma-1 isoform 2
NM_009677.6	NP_033807.2	AP-1 complex subunit gamma-1 isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AP1G1	NP_001178365.1
Neurospora crassa	NCU04121	XP_961277.2
Homo sapiens (human)	AP1G1	NP_001025178.1
Canis lupus familiaris (dog)	AP1G1	XP_003434755.1
Mus musculus (house mouse)	Ap1g1	NP_033807.2
Rattus norvegicus (Norway rat)	Ap1g1	NP_604455.1
Gallus gallus (chicken)	AP1G1	NP_001006132.1
Drosophila melanogaster (fruit fly)	AP-1gamma	NP_788890.2
Caenorhabditis elegans (roundworm)	apg-1	NP_740937.1
Xenopus tropicalis (tropical clawed frog)	ap1g1	XP_002933729.2
Schizosaccharomyces pombe (fission yeast)	apl4	NP_588559.1
Oryza sativa (rice)	Os06g0167100	NP_001056918.1
Pan troglodytes (chimpanzee)	AP1G1	XP_001171470.1
Magnaporthe oryzae (rice blast fungus)	MGG_11801	XP_003709615.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008251	XP_317218.3
Arabidopsis thaliana (thale cress)	GAMMA-ADAPTIN 1	NP_173802.1
Arabidopsis thaliana (thale cress)	AT1G60070	NP_001185270.1
Danio rerio (zebrafish)	ap1g1	NP_955976.1

Gene Ontology
Bibliography

Related articles in PubMed

A hypomorphic mutation of the gamma-1 adaptin gene (Ap1g1) causes inner ear, retina, thyroid, and testes abnormalities in mice.
Johnson KR, Gagnon LH, Chang B
Mammalian genome : official journal of the International Mammalian Genome Society27(5-6)200-12(2016 06)

Structural basis of HIV-1 Vpu-mediated BST2 antagonism via hijacking of the clathrin adaptor protein complex 1.
Jia X, Weber E, Tokarev A, Lewinski M, Rizk M, Suarez M, Guatelli J, Xiong Y
eLife3e02362(2014 Apr)

Structural basis for recruitment and activation of the AP-1 clathrin adaptor complex by Arf1.
Ren X, Farías GG, Canagarajah BJ, Bonifacino JS, Hurley JH
Cell152(4)755-67(2013 Feb)

Calcyon, a mammalian specific NEEP21 family member, interacts with adaptor protein complex 3 (AP-3) and regulates targeting of AP-3 cargoes.
Muthusamy N, Faundez V, Bergson C
Journal of neurochemistry123(1)60-72(2012 Oct)

Analysis of Gga null mice demonstrates a non-redundant role for mammalian GGA2 during development.
Govero J, Doray B, Bai H, Kornfeld S
PloS one7(1)e30184(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ap1g1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ap1g1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15615	NM_009677.6 Latest version!	Mus musculus adaptor protein complex AP-1, gamma 1 subunit (Ap1g1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu67136	NM_001301211.1 Latest version!	Mus musculus adaptor protein complex AP-1, gamma 1 subunit (Ap1g1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ap1g1 ( NM_009677.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033807.2 Mus musculus adaptor protein complex AP-1, gamma 1 subunit (Ap1g1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15615
Clone ID Related Accession (Same CDS sequence)	NM_009677.6
Accession Version	NM_009677.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	AP-1 complex subunit gamma-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY738987.1, AK036387.1, AC122807.3 and BU755831.1. On Jul 25, 2014 this sequence version replaced NM_009677.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.85241.1, SRR1660817.51748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAGCCC CCATCAGATT GCGGGAGCTG ATCCGGACCA TCCGGACAGC CCGAACCCAA 
GCTGAGGAAC GAGAAATGAT CCAGAAAGAA TGTGCTGCAA TCCGGTCCTC ATTTAGAGAA 
GAAGACAATA CATACCGGTG TCGGAATGTG GCAAAGTTAC TATATATGCA CATGCTGGGC 
TACCCTGCTC ACTTTGGACA GTTGGAGTGC CTCAAGCTAA TCGCCTCACA AAAATTCACA 
GACAAACGCA TTGGCTATTT AGGAGCAATG CTATTATTAG ATGAAAGACA AGATGTGCAT 
CTTCTCATGA CCAACTGTAT CAAGAATGAT CTTAATCATA GCACGCAGTT TGTGCAGGGG 
CTAGCACTTT GTACCCTCGG CTGCATGGGC TCCTCGGAGA TGTGCAGAGA TCTTGCGGGA 
GAGGTAGAAA AGCTTCTGAA AACCTCCAAC TCTTACTTAA GAAAAAAGGC AGCACTGTGT 
GCTGTTCACG TCATCAGGAA GGTTCCTGAA CTTATGGAGA TGTTTTTACC AGCAACAAAA 
AATTTATTGA ATGAGAAGAA CCATGGTGTC CTTCACACGT CTGTAGTCCT CCTCACAGAG 
ATGTGTGAAC GGAGCCCAGA CATGCTCGCA CATTTCAGAA AGAATGAAAA GCTTGTGCCC 
CAATTAGTTC GTATTCTAAA GAACCTCATC ATGTCCGGAT ATTCCCCAGA ACATGATGTC 
TCTGGTATCA GTGACCCCTT TTTGCAGGTA CGAATTTTGC GGTTATTAAG AATTTTAGGA 
CGGAATGACG ATGATTCAAG TGAAGCTATG AATGATATAT TAGCACAGGT TGCCACTAAT 
ACAGAGACTA GTAAAAATGT AGGAAATGCT ATTCTGTATG AGACGGTTTT GACTATCATG 
GATATTAAAT CAGAGAGTGG ATTGCGAGTT CTAGCCATAA ATATTCTTGG TCGATTCTTA 
TTGAACAATG ACAAAAATAT TAGATATGTT GCTCTGACAT CTCTGTTGAA GACTGTGCAG 
ACAGATCATA ACGCTGTACA GAGGCATAGA AGCACAATTG TGGACTGTCT GAAAGATCTG 
GATGTCTCCA TAAAAAGACG TGCAATGGAA CTGAGTTTTG CCCTGGTGAA TGGGAATAAT 
ATCCGAGGCA TGATGAAAGA ATTACTTTAT TTTCTGGATT CCTGTGAGCC AGAATTTAAA 
GCTGATTGTG CATCTGGAAT CTTCCTTGCT GCAGAAAAGT ACGCACCTTC CAAACGGTGG 
CATATAGATA CAATTATGCG TGTCTTGACA ACGGCAGGAA GTTATGTTCG CGATGATGCA 
GTTCCTAACT TGATTCAGTT AATAACCAAC AGTGTGGAGA TGCACGCTTA CACCGTCCAG 
CGCCTGTACA AGGCGATTCT CGGTGACTAT TCTCAACAAC CCTTGGTACA AGTGGCTGCG 
TGGTGTATAG GTGAATATGG CGATCTTCTT GTGTCTGGCC AGTGTGAAGA GGAAGAGCCT 
ATTCAGGTGA CAGAAGATGA AGTGCTGGAT ATTTTAGAAA GTGTCCTGAT CTCTAATATG 
TCCACCTCTG TGACAAGAGG CTATGCTCTC ACTGCCATTA TGAAGCTGTC TACCCGATTC 
ACCTGTACTG TAAACCGAAT TAAGAAAGTG GTTTCCATCT ATGGGAGCAG CATCGACGTA 
GAGCTCCAGC AGAGGGCAGT AGAGTACAAC GCACTTTTTA AGAAGTATGA CCACATGAGG 
TCTGCCCTAC TTGAAAGAAT GCCTGTCATG GAAAAAGTGA CCACAAATGG CCCTTCGGAG 
ATCGTGCAGA CAAATGGAGA GACAGAACCA GCGCCCCTAG AGACTAAACC ACCACCCTCA 
GGGCCACAAC CCACAAGCCA GGCCAATGAT CTATTGGATT TGTTGGGAGG AAATGACATA 
ACACCTGTTA TCCCAACTGC ACCTACAAGC AAACCAGCAT CAGCTGGTGG AGAACTTCTC 
GACTTGCTAG GAGACATCAC CCTGACAGGT GCTCCAGCTG CTGCTCCTAC CCCTGCCTCA 
GTGCCACAGA TATCCCAGCC CCCCTTCTTG TTGGATGGGC TTTCCTCACA GCCTCTCTTC 
AATGACATCG CTCCAGGCAT CCCCTCCATC ACAGCGTACA GTAAGAACGG CTTGAAGATA 
GAGTTCACCT TTGAACGGTC AAACACCAAC CCCAGCGTAA CAGTGATAAC GATACAGGCC 
TCTAACAGCA CAGAGCTAGA CATGACGGAC TTTGTTTTCC AGGCTGCAGT ACCAAAGACA 
TTCCAGCTGC AGCTTCTGTC TCCTAGCAGC AGCGTTGTCC CAGCCTTTAA TACAGGGACC 
ATCACACAAG TCATTAAAGT TCTGAATCCA CAGAAGCAAC AGCTGCGAAT GCGGATCAAG 
CTCACATATA ATCACAAGGG CTCGGCAATG CAAGATCTAG CAGAAGTGAA CAATTTCCCC 
CCTCAGTCCT GGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033807.2
CDS	384..2861
Translation	MPAPIRLRELIRTIRTARTQAEEREMIQKECAAIRSSFREEDNTYRCRNVAKLLYMHMLGYPAHFGQLECLKLIASQKFTDKRIGYLGAMLLLDERQDVHLLMTNCIKNDLNHSTQFVQGLALCTLGCMGSSEMCRDLAGEVEKLLKTSNSYLRKKAALCAVHVIRKVPELMEMFLPATKNLLNEKNHGVLHTSVVLLTEMCERSPDMLAHFRKNEKLVPQLVRILKNLIMSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATNTETSKNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHRSTIVDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRWHIDTIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQQPLVQVAAWCIGEYGDLLVSGQCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDVELQQRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPSEIVQTNGETEPAPLETKPPPSGPQPTSQANDLLDLLGGNDITPVIPTAPTSKPASAGGELLDLLGDITLTGAPAAAPTPASVPQISQPPFLLDGLSSQPLFNDIAPGIPSITAYSKNGLKIEFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSSSVVPAFNTGTITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSWQ

Target ORF information:

RefSeq Version	NM_009677.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor protein complex AP-1, gamma 1 subunit (Ap1g1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009677.6

ORF Insert Sequence:

ATGCCAGCCC CCATCAGATT GCGGGAGCTG ATCCGGACCA TCCGGACAGC CCGAACCCAA 
GCTGAGGAAC GAGAAATGAT CCAGAAAGAA TGTGCTGCAA TCCGGTCCTC ATTTAGAGAA 
GAAGACAATA CATACCGGTG TCGGAATGTG GCAAAGTTAC TATATATGCA CATGCTGGGC 
TACCCTGCTC ACTTTGGACA GTTGGAGTGC CTCAAGCTAA TCGCCTCACA AAAATTCACA 
GACAAACGCA TTGGCTATTT AGGAGCAATG CTATTATTAG ATGAAAGACA AGATGTGCAT 
CTTCTCATGA CCAACTGTAT CAAGAATGAT CTTAATCATA GCACGCAGTT TGTGCAGGGG 
CTAGCACTTT GTACCCTCGG CTGCATGGGC TCCTCGGAGA TGTGCAGAGA TCTTGCGGGA 
GAGGTAGAAA AGCTTCTGAA AACCTCCAAC TCTTACTTAA GAAAAAAGGC AGCACTGTGT 
GCTGTTCACG TCATCAGGAA GGTTCCTGAA CTTATGGAGA TGTTTTTACC AGCAACAAAA 
AATTTATTGA ATGAGAAGAA CCATGGTGTC CTTCACACGT CTGTAGTCCT CCTCACAGAG 
ATGTGTGAAC GGAGCCCAGA CATGCTCGCA CATTTCAGAA AGAATGAAAA GCTTGTGCCC 
CAATTAGTTC GTATTCTAAA GAACCTCATC ATGTCCGGAT ATTCCCCAGA ACATGATGTC 
TCTGGTATCA GTGACCCCTT TTTGCAGGTA CGAATTTTGC GGTTATTAAG AATTTTAGGA 
CGGAATGACG ATGATTCAAG TGAAGCTATG AATGATATAT TAGCACAGGT TGCCACTAAT 
ACAGAGACTA GTAAAAATGT AGGAAATGCT ATTCTGTATG AGACGGTTTT GACTATCATG 
GATATTAAAT CAGAGAGTGG ATTGCGAGTT CTAGCCATAA ATATTCTTGG TCGATTCTTA 
TTGAACAATG ACAAAAATAT TAGATATGTT GCTCTGACAT CTCTGTTGAA GACTGTGCAG 
ACAGATCATA ACGCTGTACA GAGGCATAGA AGCACAATTG TGGACTGTCT GAAAGATCTG 
GATGTCTCCA TAAAAAGACG TGCAATGGAA CTGAGTTTTG CCCTGGTGAA TGGGAATAAT 
ATCCGAGGCA TGATGAAAGA ATTACTTTAT TTTCTGGATT CCTGTGAGCC AGAATTTAAA 
GCTGATTGTG CATCTGGAAT CTTCCTTGCT GCAGAAAAGT ACGCACCTTC CAAACGGTGG 
CATATAGATA CAATTATGCG TGTCTTGACA ACGGCAGGAA GTTATGTTCG CGATGATGCA 
GTTCCTAACT TGATTCAGTT AATAACCAAC AGTGTGGAGA TGCACGCTTA CACCGTCCAG 
CGCCTGTACA AGGCGATTCT CGGTGACTAT TCTCAACAAC CCTTGGTACA AGTGGCTGCG 
TGGTGTATAG GTGAATATGG CGATCTTCTT GTGTCTGGCC AGTGTGAAGA GGAAGAGCCT 
ATTCAGGTGA CAGAAGATGA AGTGCTGGAT ATTTTAGAAA GTGTCCTGAT CTCTAATATG 
TCCACCTCTG TGACAAGAGG CTATGCTCTC ACTGCCATTA TGAAGCTGTC TACCCGATTC 
ACCTGTACTG TAAACCGAAT TAAGAAAGTG GTTTCCATCT ATGGGAGCAG CATCGACGTA 
GAGCTCCAGC AGAGGGCAGT AGAGTACAAC GCACTTTTTA AGAAGTATGA CCACATGAGG 
TCTGCCCTAC TTGAAAGAAT GCCTGTCATG GAAAAAGTGA CCACAAATGG CCCTTCGGAG 
ATCGTGCAGA CAAATGGAGA GACAGAACCA GCGCCCCTAG AGACTAAACC ACCACCCTCA 
GGGCCACAAC CCACAAGCCA GGCCAATGAT CTATTGGATT TGTTGGGAGG AAATGACATA 
ACACCTGTTA TCCCAACTGC ACCTACAAGC AAACCAGCAT CAGCTGGTGG AGAACTTCTC 
GACTTGCTAG GAGACATCAC CCTGACAGGT GCTCCAGCTG CTGCTCCTAC CCCTGCCTCA 
GTGCCACAGA TATCCCAGCC CCCCTTCTTG TTGGATGGGC TTTCCTCACA GCCTCTCTTC 
AATGACATCG CTCCAGGCAT CCCCTCCATC ACAGCGTACA GTAAGAACGG CTTGAAGATA 
GAGTTCACCT TTGAACGGTC AAACACCAAC CCCAGCGTAA CAGTGATAAC GATACAGGCC 
TCTAACAGCA CAGAGCTAGA CATGACGGAC TTTGTTTTCC AGGCTGCAGT ACCAAAGACA 
TTCCAGCTGC AGCTTCTGTC TCCTAGCAGC AGCGTTGTCC CAGCCTTTAA TACAGGGACC 
ATCACACAAG TCATTAAAGT TCTGAATCCA CAGAAGCAAC AGCTGCGAAT GCGGATCAAG 
CTCACATATA ATCACAAGGG CTCGGCAATG CAAGATCTAG CAGAAGTGAA CAATTTCCCC 
CCTCAGTCCT GGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap1g1 ( NM_001301211.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288140.1 Mus musculus adaptor protein complex AP-1, gamma 1 subunit (Ap1g1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu67136
Clone ID Related Accession (Same CDS sequence)	NM_001301211.1
Accession Version	NM_001301211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Mus musculus(house mouse)
Product	AP-1 complex subunit gamma-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY738987.1, X54424.1, AC122807.3 and BU755831.1. On Jul 25, 2014 this sequence version replaced XM_006530584.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.101410.1, SRR1660821.138487.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAGCCC CCATCAGATT GCGGGAGCTG ATCCGGACCA TCCGGACAGC CCGAACCCAA 
GCTGAGGAAC GAGAAATGAT CCAGAAAGAA TGTGCTGCAA TCCGGTCCTC ATTTAGAGAA 
GAAGACAATA CATACCGGTG TCGGAATGTG GCAAAGTTAC TATATATGCA CATGCTGGGC 
TACCCTGCTC ACTTTGGACA GTTGGAGTGC CTCAAGCTAA TCGCCTCACA AAAATTCACA 
GACAAACGCA TTGGCTATTT AGGAGCAATG CTATTATTAG ATGAAAGACA AGATGTGCAT 
CTTCTCATGA CCAACTGTAT CAAGAATGAT CTTAATCATA GCACGCAGTT TGTGCAGGGG 
CTAGCACTTT GTACCCTCGG CTGCATGGGC TCCTCGGAGA TGTGCAGAGA TCTTGCGGGA 
GAGGTAGAAA AGCTTCTGAA AACCTCCAAC TCTTACTTAA GAAAAAAGGC AGCACTGTGT 
GCTGTTCACG TCATCAGGAA GGTTCCTGAA CTTATGGAGA TGTTTTTACC AGCAACAAAA 
AATTTATTGA ATGAGAAGAA CCATGGTGTC CTTCACACGT CTGTAGTCCT CCTCACAGAG 
ATGTGTGAAC GGAGCCCAGA CATGCTCGCA CATTTCAGAA AGCTTGTGCC CCAATTAGTT 
CGTATTCTAA AGAACCTCAT CATGTCCGGA TATTCCCCAG AACATGATGT CTCTGGTATC 
AGTGACCCCT TTTTGCAGGT ACGAATTTTG CGGTTATTAA GAATTTTAGG ACGGAATGAC 
GATGATTCAA GTGAAGCTAT GAATGATATA TTAGCACAGG TTGCCACTAA TACAGAGACT 
AGTAAAAATG TAGGAAATGC TATTCTGTAT GAGACGGTTT TGACTATCAT GGATATTAAA 
TCAGAGAGTG GATTGCGAGT TCTAGCCATA AATATTCTTG GTCGATTCTT ATTGAACAAT 
GACAAAAATA TTAGATATGT TGCTCTGACA TCTCTGTTGA AGACTGTGCA GACAGATCAT 
AACGCTGTAC AGAGGCATAG AAGCACAATT GTGGACTGTC TGAAAGATCT GGATGTCTCC 
ATAAAAAGAC GTGCAATGGA ACTGAGTTTT GCCCTGGTGA ATGGGAATAA TATCCGAGGC 
ATGATGAAAG AATTACTTTA TTTTCTGGAT TCCTGTGAGC CAGAATTTAA AGCTGATTGT 
GCATCTGGAA TCTTCCTTGC TGCAGAAAAG TACGCACCTT CCAAACGGTG GCATATAGAT 
ACAATTATGC GTGTCTTGAC AACGGCAGGA AGTTATGTTC GCGATGATGC AGTTCCTAAC 
TTGATTCAGT TAATAACCAA CAGTGTGGAG ATGCACGCTT ACACCGTCCA GCGCCTGTAC 
AAGGCGATTC TCGGTGACTA TTCTCAACAA CCCTTGGTAC AAGTGGCTGC GTGGTGTATA 
GGTGAATATG GCGATCTTCT TGTGTCTGGC CAGTGTGAAG AGGAAGAGCC TATTCAGGTG 
ACAGAAGATG AAGTGCTGGA TATTTTAGAA AGTGTCCTGA TCTCTAATAT GTCCACCTCT 
GTGACAAGAG GCTATGCTCT CACTGCCATT ATGAAGCTGT CTACCCGATT CACCTGTACT 
GTAAACCGAA TTAAGAAAGT GGTTTCCATC TATGGGAGCA GCATCGACGT AGAGCTCCAG 
CAGAGGGCAG TAGAGTACAA CGCACTTTTT AAGAAGTATG ACCACATGAG GTCTGCCCTA 
CTTGAAAGAA TGCCTGTCAT GGAAAAAGTG ACCACAAATG GCCCTTCGGA GATCGTGCAG 
ACAAATGGAG AGACAGAACC AGCGCCCCTA GAGACTAAAC CACCACCCTC AGGGCCACAA 
CCCACAAGCC AGGCCAATGA TCTATTGGAT TTGTTGGGAG GAAATGACAT AACACCTGTT 
ATCCCAACTG CACCTACAAG CAAACCAGCA TCAGCTGGTG GAGAACTTCT CGACTTGCTA 
GGAGACATCA CCCTGACAGG TGCTCCAGCT GCTGCTCCTA CCCCTGCCTC AGTGCCACAG 
ATATCCCAGC CCCCCTTCTT GTTGGATGGG CTTTCCTCAC AGCCTCTCTT CAATGACATC 
GCTCCAGGCA TCCCCTCCAT CACAGCGTAC AGTAAGAACG GCTTGAAGAT AGAGTTCACC 
TTTGAACGGT CAAACACCAA CCCCAGCGTA ACAGTGATAA CGATACAGGC CTCTAACAGC 
ACAGAGCTAG ACATGACGGA CTTTGTTTTC CAGGCTGCAG TACCAAAGAC ATTCCAGCTG 
CAGCTTCTGT CTCCTAGCAG CAGCGTTGTC CCAGCCTTTA ATACAGGGAC CATCACACAA 
GTCATTAAAG TTCTGAATCC ACAGAAGCAA CAGCTGCGAA TGCGGATCAA GCTCACATAT 
AATCACAAGG GCTCGGCAAT GCAAGATCTA GCAGAAGTGA ACAATTTCCC CCCTCAGTCC 
TGGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288140.1
CDS	384..2852
Translation	MPAPIRLRELIRTIRTARTQAEEREMIQKECAAIRSSFREEDNTYRCRNVAKLLYMHMLGYPAHFGQLECLKLIASQKFTDKRIGYLGAMLLLDERQDVHLLMTNCIKNDLNHSTQFVQGLALCTLGCMGSSEMCRDLAGEVEKLLKTSNSYLRKKAALCAVHVIRKVPELMEMFLPATKNLLNEKNHGVLHTSVVLLTEMCERSPDMLAHFRKLVPQLVRILKNLIMSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATNTETSKNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHRSTIVDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRWHIDTIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQQPLVQVAAWCIGEYGDLLVSGQCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDVELQQRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPSEIVQTNGETEPAPLETKPPPSGPQPTSQANDLLDLLGGNDITPVIPTAPTSKPASAGGELLDLLGDITLTGAPAAAPTPASVPQISQPPFLLDGLSSQPLFNDIAPGIPSITAYSKNGLKIEFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSSSVVPAFNTGTITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSWQ

Target ORF information:

RefSeq Version	NM_001301211.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor protein complex AP-1, gamma 1 subunit (Ap1g1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301211.1

ORF Insert Sequence:

ATGCCAGCCC CCATCAGATT GCGGGAGCTG ATCCGGACCA TCCGGACAGC CCGAACCCAA 
GCTGAGGAAC GAGAAATGAT CCAGAAAGAA TGTGCTGCAA TCCGGTCCTC ATTTAGAGAA 
GAAGACAATA CATACCGGTG TCGGAATGTG GCAAAGTTAC TATATATGCA CATGCTGGGC 
TACCCTGCTC ACTTTGGACA GTTGGAGTGC CTCAAGCTAA TCGCCTCACA AAAATTCACA 
GACAAACGCA TTGGCTATTT AGGAGCAATG CTATTATTAG ATGAAAGACA AGATGTGCAT 
CTTCTCATGA CCAACTGTAT CAAGAATGAT CTTAATCATA GCACGCAGTT TGTGCAGGGG 
CTAGCACTTT GTACCCTCGG CTGCATGGGC TCCTCGGAGA TGTGCAGAGA TCTTGCGGGA 
GAGGTAGAAA AGCTTCTGAA AACCTCCAAC TCTTACTTAA GAAAAAAGGC AGCACTGTGT 
GCTGTTCACG TCATCAGGAA GGTTCCTGAA CTTATGGAGA TGTTTTTACC AGCAACAAAA 
AATTTATTGA ATGAGAAGAA CCATGGTGTC CTTCACACGT CTGTAGTCCT CCTCACAGAG 
ATGTGTGAAC GGAGCCCAGA CATGCTCGCA CATTTCAGAA AGCTTGTGCC CCAATTAGTT 
CGTATTCTAA AGAACCTCAT CATGTCCGGA TATTCCCCAG AACATGATGT CTCTGGTATC 
AGTGACCCCT TTTTGCAGGT ACGAATTTTG CGGTTATTAA GAATTTTAGG ACGGAATGAC 
GATGATTCAA GTGAAGCTAT GAATGATATA TTAGCACAGG TTGCCACTAA TACAGAGACT 
AGTAAAAATG TAGGAAATGC TATTCTGTAT GAGACGGTTT TGACTATCAT GGATATTAAA 
TCAGAGAGTG GATTGCGAGT TCTAGCCATA AATATTCTTG GTCGATTCTT ATTGAACAAT 
GACAAAAATA TTAGATATGT TGCTCTGACA TCTCTGTTGA AGACTGTGCA GACAGATCAT 
AACGCTGTAC AGAGGCATAG AAGCACAATT GTGGACTGTC TGAAAGATCT GGATGTCTCC 
ATAAAAAGAC GTGCAATGGA ACTGAGTTTT GCCCTGGTGA ATGGGAATAA TATCCGAGGC 
ATGATGAAAG AATTACTTTA TTTTCTGGAT TCCTGTGAGC CAGAATTTAA AGCTGATTGT 
GCATCTGGAA TCTTCCTTGC TGCAGAAAAG TACGCACCTT CCAAACGGTG GCATATAGAT 
ACAATTATGC GTGTCTTGAC AACGGCAGGA AGTTATGTTC GCGATGATGC AGTTCCTAAC 
TTGATTCAGT TAATAACCAA CAGTGTGGAG ATGCACGCTT ACACCGTCCA GCGCCTGTAC 
AAGGCGATTC TCGGTGACTA TTCTCAACAA CCCTTGGTAC AAGTGGCTGC GTGGTGTATA 
GGTGAATATG GCGATCTTCT TGTGTCTGGC CAGTGTGAAG AGGAAGAGCC TATTCAGGTG 
ACAGAAGATG AAGTGCTGGA TATTTTAGAA AGTGTCCTGA TCTCTAATAT GTCCACCTCT 
GTGACAAGAG GCTATGCTCT CACTGCCATT ATGAAGCTGT CTACCCGATT CACCTGTACT 
GTAAACCGAA TTAAGAAAGT GGTTTCCATC TATGGGAGCA GCATCGACGT AGAGCTCCAG 
CAGAGGGCAG TAGAGTACAA CGCACTTTTT AAGAAGTATG ACCACATGAG GTCTGCCCTA 
CTTGAAAGAA TGCCTGTCAT GGAAAAAGTG ACCACAAATG GCCCTTCGGA GATCGTGCAG 
ACAAATGGAG AGACAGAACC AGCGCCCCTA GAGACTAAAC CACCACCCTC AGGGCCACAA 
CCCACAAGCC AGGCCAATGA TCTATTGGAT TTGTTGGGAG GAAATGACAT AACACCTGTT 
ATCCCAACTG CACCTACAAG CAAACCAGCA TCAGCTGGTG GAGAACTTCT CGACTTGCTA 
GGAGACATCA CCCTGACAGG TGCTCCAGCT GCTGCTCCTA CCCCTGCCTC AGTGCCACAG 
ATATCCCAGC CCCCCTTCTT GTTGGATGGG CTTTCCTCAC AGCCTCTCTT CAATGACATC 
GCTCCAGGCA TCCCCTCCAT CACAGCGTAC AGTAAGAACG GCTTGAAGAT AGAGTTCACC 
TTTGAACGGT CAAACACCAA CCCCAGCGTA ACAGTGATAA CGATACAGGC CTCTAACAGC 
ACAGAGCTAG ACATGACGGA CTTTGTTTTC CAGGCTGCAG TACCAAAGAC ATTCCAGCTG 
CAGCTTCTGT CTCCTAGCAG CAGCGTTGTC CCAGCCTTTA ATACAGGGAC CATCACACAA 
GTCATTAAAG TTCTGAATCC ACAGAAGCAA CAGCTGCGAA TGCGGATCAA GCTCACATAT 
AATCACAAGG GCTCGGCAAT GCAAGATCTA GCAGAAGTGA ACAATTTCCC CCCTCAGTCC 
TGGCAATGA