Helb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Helb
Entrez Gene ID	117599
Full Name	helicase (DNA) B
Synonyms	AI447783,D10Ertd664e
General protein information	Preferred Names helicase (DNA) B Names DNA helicase B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 D2\|10 67.94 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017313781.2	XP_017169270.1	DNA helicase B isoform X1
XM_017313781.1	XP_017169270.1	DNA helicase B isoform X1
NM_080446.2	NP_536694.2	DNA helicase B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HELB	XP_850757.2
Rattus norvegicus (Norway rat)	Helb	XP_006241488.1
Xenopus tropicalis (tropical clawed frog)	helb	XP_002937585.2
Homo sapiens (human)	HELB	NP_387467.2
Bos taurus (cattle)	HELB	NP_001193111.1
Danio rerio (zebrafish)	helb	XP_001336777.3
Gallus gallus (chicken)	HELB	XP_416077.3
Pan troglodytes (chimpanzee)	HELB	XP_001162205.1
Macaca mulatta (Rhesus monkey)	HELB	XP_001117092.1
Mus musculus (house mouse)	Helb	NP_536694.2

Gene Ontology
Bibliography

Related articles in PubMed

HELB Is a Feedback Inhibitor of DNA End Resection.
Tkáč J, Xu G, Adhikary H, Young JTF, Gallo D, Escribano-Díaz C, Krietsch J, Orthwein A, Munro M, Sol W, Al-Hakim A, Lin ZY, Jonkers J, Borst P, Brown GW, Gingras AC, Rottenberg S, Masson JY, Durocher D
Molecular cell61(3)405-418(2016 Feb)

Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDnas identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Kitamura H, Nakagawa T, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes11(2)127-35(2004 Apr)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Molecular cloning of a cDNA encoding mouse DNA helicase B, which has homology to Escherichia coli RecD protein, and identification of a mutation in the DNA helicase B from tsFT848 temperature-sensitive DNA replication mutant cells.
Tada S, Kobayashi T, Omori A, Kusa Y, Okumura N, Kodaira H, Ishimi Y, Seki M, Enomoto T
Nucleic acids research29(18)3835-40(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Helb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Helb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu77418	XM_017313781.2 Latest version!	Mus musculus helicase (DNA) B (Helb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu09652	NM_080446.2 Latest version!	Mus musculus helicase (DNA) B (Helb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu77418	XM_017313781.1	Mus musculus helicase (DNA) B (Helb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Helb ( XM_017313781.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169270.1 Mus musculus helicase (DNA) B (Helb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77418
Clone ID Related Accession (Same CDS sequence)	XM_017313781.2 , XM_017313781.1
Accession Version	XM_017313781.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	DNA helicase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGGC AGGATCGTCT GCGCGAGCTC CTGGGCCCTC TGCACCCGTA CAAGAGCGAC 
GACGAGGAGG AGGATTGTGC GCAGGAGGAG GAGGGGGAGC AGGAGGAGGA GTTTGTGGAT 
GCCGAGGAAC TCTGCAGCGG GGGCATCAAG GCGGGCAGCC TCCCTGGGCG CGCGCGAGTG 
TCTATCCCTG ATGAGTATAC TAAAGAGAAA TGTACCGTGT ACGGACGTTT CCCACTCAAA 
GGTCCGTGGT GGCGCGTGAA GGTCCAGGTC CTGAAACCCC AGCGATCCAG GAGCTATCAA 
GTTCAAGGCT TTCCGGCATA CTTTCTACAG GTTGATATGT CGCCACCAGA TCAAAAACAA 
ATCTGTTCGC TTTTTCTTAA AGAATGCAAC TTAGCCTCTG AGCGCATACA GGAATTTTTA 
AAATGGGTAG AAAAAGTGTC AAGCTTTGAA AATCTACACT TTGAAAATCT TTGGGAAACA 
TTAAGGCTTT TCTACAGAGA AACCGAAAAG AAAGATAAAA AGCTGTCTAC ACCAAGGGAG 
CAGCAGGGTG AGGAGATGAG AGTGGAAAAA TCATTCGCAT TTATAAGTGC AATGGTAGCT 
CTGCAATTCC CCAAAGTCAT GGAATTCCTT CCATCGCTTT TCCCTCGACA CTTCAAAAGG 
CTCATCAGCT CGAGTTCTGA TTGGGTGCTG GGATGCATAG AAGATGTTCT GGGTACACAG 
CCGTGGAAGC TGGGCTTCAG GAGGATAACC TACAGAGAAA TGAAACTAGT GCGCTGTGAG 
GCCAGCTGGA CAGCGTTCAG TCAGTGCCCC TCGCTGCTCC AGCTGATGAC GCCCCTGCAG 
AAGAATGCAT TGGTCATATA TTCTAAGCTG AGGCAGACAT GCAGAGAAGA CGGACACACG 
TACATTGAAG TAAAAGACTT GACCTCGGGC TTGTCGGAGC ATATGTCATT CGAGGAAGCG 
TGTCAGTCTC TGGCGTTTCT GAAGGACATT GACGTGGTCA TTTATGAGAA GGACTATGTC 
TTTCTTTCTG AACTGTACGA GGCCGAGCAA GACATCGCCT CCTCCATCTG CGAGCTGATG 
AGCAGACCTC CGTGGCACTT AAAAGTGGAC GTGAAGAACG TCCTGGCGTC AATTAGAGGC 
GCAAAGCCCA ACGATCCAGG AAGTGCTGAG GCAGTGGAAG GGAGCAAGCC GGAGGAGGTG 
GGGTCAGAGC AGGGTGACAG TGTTTTAGAC GCCCAAGATG GCGATGACCA TGTTAGAAGC 
AATGGCGAAC ACGTCGCAAA TGCAGAAATA AACGACGTCC CACTGGATCA AGATCAGGTG 
GTCGCCCTGG AGACGATCTG CGCCAACGCG GTGACTGTCC TCAGTGGGAA AGGCGGCTGT 
GGGAAGACCA CCATTGTTAG CCGTCTTTTT AAGCACATGG AACATCTGGA AGAAACGGAA 
GTGCAGCAAG CCTGCGAGGA TTTTGAACAA GACCAGGAAG CCTCAGAAGA GTGGCTTGAC 
TGTCCTAAGC AGAGCCCAGC CGGTGTGGAC AAGGCTGTAG AAGTCTTGCT CACCGCACCT 
ACAGGGAAGG CTGCTGGCTT ATTGAGGCAG AGGACCGATC TTCCGGCTTA CACGCTGTGC 
CAGGTCAATT ACAGCTTCTA TATGTGGAAA ACGAAAAATG AGGTGGACAA GCCGTGGAAA 
TTCTCCACGG TTCGAGTTCT GGTTGTGGAC GAAGGGAGTT TGGTGTCTGT AGGGATCTTC 
AAGTCAGTGT TACAGTTGCT GTGCAAACAC TCCAAGCTGT CCAAGCTCAT CATCCTTGGT 
GATGTTAGAC AGTTGCCCAG TATTGAACCT GGTAACATGC TGCAGGATGT CTTTGAGACT 
CTTAAGTCAA GACAGTGTGC CATCGAACTA AAGACGAACC ACAGGACAGA GTCTCAGCTG 
ATTGTGGACA ATGCTACAAG AATCTCCAGA CGTCAGTTTC CAAAATTTGA TGCTGAACTA 
AATATCTGTG GTAATCCTAC ATTACCTCTC TCCATTCAAG ATAAGACATT TATCTTTGTG 
AGACTCCCTG AAGAAGACAG CAGATCTCAG TCGTCTAAAG GTGAACACCG ATCTAATTTA 
TATACTGCAG TTAAAACTTT GCTTCAAGGA AAGGACTTCT GCTCTTTTGA AAGTTCAAAA 
ACATCGCAAT TTATTGCATT TAGAAGGCAA GACTGTGACC TCATCAATGA CTGCTGCTGC 
AAGCACTACA CAGGCCACCT CATCAAAGAC CATGAGAAGA AACTCATCTT TGCCGTCGGT 
GATAAGATCT GCTGTACCCG GAATGCATAC CTCTCAGATT TACTCCCCGA CAAAGACCAG 
GAAGCCGAGG GGAAAGGCTA TGGAGACGCC CCTGACGACG ATGCTAAAAT CAAACAGGAT 
TTCGAAAGCA GCACCCGCCT GTGCAACGGG GAAATATTTT TCATAACACG GGATGTAACT 
GACGTAACTT TCAAAAGAAA AAGACTTCTG ACCATTAATA ACGAGGCCGG TTTGGAAGTG 
ACTGTGGACT TTAGCAAACT AATGGCAAAC TGTCAGATCA AGCATGCCTG GGCAAGGACC 
ATTCATACTT TTCAGCTTGA CTCCAGCAGG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169270.1
CDS	149..2764
Translation	MARQDRLRELLGPLHPYKSDDEEEDCAQEEEGEQEEEFVDAEELCSGGIKAGSLPGRARVSIPDEYTKEKCTVYGRFPLKGPWWRVKVQVLKPQRSRSYQVQGFPAYFLQVDMSPPDQKQICSLFLKECNLASERIQEFLKWVEKVSSFENLHFENLWETLRLFYRETEKKDKKLSTPREQQGEEMRVEKSFAFISAMVALQFPKVMEFLPSLFPRHFKRLISSSSDWVLGCIEDVLGTQPWKLGFRRITYREMKLVRCEASWTAFSQCPSLLQLMTPLQKNALVIYSKLRQTCREDGHTYIEVKDLTSGLSEHMSFEEACQSLAFLKDIDVVIYEKDYVFLSELYEAEQDIASSICELMSRPPWHLKVDVKNVLASIRGAKPNDPGSAEAVEGSKPEEVGSEQGDSVLDAQDGDDHVRSNGEHVANAEINDVPLDQDQVVALETICANAVTVLSGKGGCGKTTIVSRLFKHMEHLEETEVQQACEDFEQDQEASEEWLDCPKQSPAGVDKAVEVLLTAPTGKAAGLLRQRTDLPAYTLCQVNYSFYMWKTKNEVDKPWKFSTVRVLVVDEGSLVSVGIFKSVLQLLCKHSKLSKLIILGDVRQLPSIEPGNMLQDVFETLKSRQCAIELKTNHRTESQLIVDNATRISRRQFPKFDAELNICGNPTLPLSIQDKTFIFVRLPEEDSRSQSSKGEHRSNLYTAVKTLLQGKDFCSFESSKTSQFIAFRRQDCDLINDCCCKHYTGHLIKDHEKKLIFAVGDKICCTRNAYLSDLLPDKDQEAEGKGYGDAPDDDAKIKQDFESSTRLCNGEIFFITRDVTDVTFKRKRLLTINNEAGLEVTVDFSKLMANCQIKHAWARTIHTFQLDSSRA

Target ORF information:

RefSeq Version	XM_017313781.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus helicase (DNA) B (Helb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313781.2

ORF Insert Sequence:

ATGGCTAGGC AGGATCGTCT GCGCGAGCTC CTGGGCCCTC TGCACCCGTA CAAGAGCGAC 
GACGAGGAGG AGGATTGTGC GCAGGAGGAG GAGGGGGAGC AGGAGGAGGA GTTTGTGGAT 
GCCGAGGAAC TCTGCAGCGG GGGCATCAAG GCGGGCAGCC TCCCTGGGCG CGCGCGAGTG 
TCTATCCCTG ATGAGTATAC TAAAGAGAAA TGTACCGTGT ACGGACGTTT CCCACTCAAA 
GGTCCGTGGT GGCGCGTGAA GGTCCAGGTC CTGAAACCCC AGCGATCCAG GAGCTATCAA 
GTTCAAGGCT TTCCGGCATA CTTTCTACAG GTTGATATGT CGCCACCAGA TCAAAAACAA 
ATCTGTTCGC TTTTTCTTAA AGAATGCAAC TTAGCCTCTG AGCGCATACA GGAATTTTTA 
AAATGGGTAG AAAAAGTGTC AAGCTTTGAA AATCTACACT TTGAAAATCT TTGGGAAACA 
TTAAGGCTTT TCTACAGAGA AACCGAAAAG AAAGATAAAA AGCTGTCTAC ACCAAGGGAG 
CAGCAGGGTG AGGAGATGAG AGTGGAAAAA TCATTCGCAT TTATAAGTGC AATGGTAGCT 
CTGCAATTCC CCAAAGTCAT GGAATTCCTT CCATCGCTTT TCCCTCGACA CTTCAAAAGG 
CTCATCAGCT CGAGTTCTGA TTGGGTGCTG GGATGCATAG AAGATGTTCT GGGTACACAG 
CCGTGGAAGC TGGGCTTCAG GAGGATAACC TACAGAGAAA TGAAACTAGT GCGCTGTGAG 
GCCAGCTGGA CAGCGTTCAG TCAGTGCCCC TCGCTGCTCC AGCTGATGAC GCCCCTGCAG 
AAGAATGCAT TGGTCATATA TTCTAAGCTG AGGCAGACAT GCAGAGAAGA CGGACACACG 
TACATTGAAG TAAAAGACTT GACCTCGGGC TTGTCGGAGC ATATGTCATT CGAGGAAGCG 
TGTCAGTCTC TGGCGTTTCT GAAGGACATT GACGTGGTCA TTTATGAGAA GGACTATGTC 
TTTCTTTCTG AACTGTACGA GGCCGAGCAA GACATCGCCT CCTCCATCTG CGAGCTGATG 
AGCAGACCTC CGTGGCACTT AAAAGTGGAC GTGAAGAACG TCCTGGCGTC AATTAGAGGC 
GCAAAGCCCA ACGATCCAGG AAGTGCTGAG GCAGTGGAAG GGAGCAAGCC GGAGGAGGTG 
GGGTCAGAGC AGGGTGACAG TGTTTTAGAC GCCCAAGATG GCGATGACCA TGTTAGAAGC 
AATGGCGAAC ACGTCGCAAA TGCAGAAATA AACGACGTCC CACTGGATCA AGATCAGGTG 
GTCGCCCTGG AGACGATCTG CGCCAACGCG GTGACTGTCC TCAGTGGGAA AGGCGGCTGT 
GGGAAGACCA CCATTGTTAG CCGTCTTTTT AAGCACATGG AACATCTGGA AGAAACGGAA 
GTGCAGCAAG CCTGCGAGGA TTTTGAACAA GACCAGGAAG CCTCAGAAGA GTGGCTTGAC 
TGTCCTAAGC AGAGCCCAGC CGGTGTGGAC AAGGCTGTAG AAGTCTTGCT CACCGCACCT 
ACAGGGAAGG CTGCTGGCTT ATTGAGGCAG AGGACCGATC TTCCGGCTTA CACGCTGTGC 
CAGGTCAATT ACAGCTTCTA TATGTGGAAA ACGAAAAATG AGGTGGACAA GCCGTGGAAA 
TTCTCCACGG TTCGAGTTCT GGTTGTGGAC GAAGGGAGTT TGGTGTCTGT AGGGATCTTC 
AAGTCAGTGT TACAGTTGCT GTGCAAACAC TCCAAGCTGT CCAAGCTCAT CATCCTTGGT 
GATGTTAGAC AGTTGCCCAG TATTGAACCT GGTAACATGC TGCAGGATGT CTTTGAGACT 
CTTAAGTCAA GACAGTGTGC CATCGAACTA AAGACGAACC ACAGGACAGA GTCTCAGCTG 
ATTGTGGACA ATGCTACAAG AATCTCCAGA CGTCAGTTTC CAAAATTTGA TGCTGAACTA 
AATATCTGTG GTAATCCTAC ATTACCTCTC TCCATTCAAG ATAAGACATT TATCTTTGTG 
AGACTCCCTG AAGAAGACAG CAGATCTCAG TCGTCTAAAG GTGAACACCG ATCTAATTTA 
TATACTGCAG TTAAAACTTT GCTTCAAGGA AAGGACTTCT GCTCTTTTGA AAGTTCAAAA 
ACATCGCAAT TTATTGCATT TAGAAGGCAA GACTGTGACC TCATCAATGA CTGCTGCTGC 
AAGCACTACA CAGGCCACCT CATCAAAGAC CATGAGAAGA AACTCATCTT TGCCGTCGGT 
GATAAGATCT GCTGTACCCG GAATGCATAC CTCTCAGATT TACTCCCCGA CAAAGACCAG 
GAAGCCGAGG GGAAAGGCTA TGGAGACGCC CCTGACGACG ATGCTAAAAT CAAACAGGAT 
TTCGAAAGCA GCACCCGCCT GTGCAACGGG GAAATATTTT TCATAACACG GGATGTAACT 
GACGTAACTT TCAAAAGAAA AAGACTTCTG ACCATTAATA ACGAGGCCGG TTTGGAAGTG 
ACTGTGGACT TTAGCAAACT AATGGCAAAC TGTCAGATCA AGCATGCCTG GGCAAGGACC 
ATTCATACTT TTCAGCTTGA CTCCAGCAGG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Helb ( XM_017313781.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169270.1 Mus musculus helicase (DNA) B (Helb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77418
Clone ID Related Accession (Same CDS sequence)	XM_017313781.2 , XM_017313781.1
Accession Version	XM_017313781.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	DNA helicase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGGC AGGATCGTCT GCGCGAGCTC CTGGGCCCTC TGCACCCGTA CAAGAGCGAC 
GACGAGGAGG AGGATTGTGC GCAGGAGGAG GAGGGGGAGC AGGAGGAGGA GTTTGTGGAT 
GCCGAGGAAC TCTGCAGCGG GGGCATCAAG GCGGGCAGCC TCCCTGGGCG CGCGCGAGTG 
TCTATCCCTG ATGAGTATAC TAAAGAGAAA TGTACCGTGT ACGGACGTTT CCCACTCAAA 
GGTCCGTGGT GGCGCGTGAA GGTCCAGGTC CTGAAACCCC AGCGATCCAG GAGCTATCAA 
GTTCAAGGCT TTCCGGCATA CTTTCTACAG GTTGATATGT CGCCACCAGA TCAAAAACAA 
ATCTGTTCGC TTTTTCTTAA AGAATGCAAC TTAGCCTCTG AGCGCATACA GGAATTTTTA 
AAATGGGTAG AAAAAGTGTC AAGCTTTGAA AATCTACACT TTGAAAATCT TTGGGAAACA 
TTAAGGCTTT TCTACAGAGA AACCGAAAAG AAAGATAAAA AGCTGTCTAC ACCAAGGGAG 
CAGCAGGGTG AGGAGATGAG AGTGGAAAAA TCATTCGCAT TTATAAGTGC AATGGTAGCT 
CTGCAATTCC CCAAAGTCAT GGAATTCCTT CCATCGCTTT TCCCTCGACA CTTCAAAAGG 
CTCATCAGCT CGAGTTCTGA TTGGGTGCTG GGATGCATAG AAGATGTTCT GGGTACACAG 
CCGTGGAAGC TGGGCTTCAG GAGGATAACC TACAGAGAAA TGAAACTAGT GCGCTGTGAG 
GCCAGCTGGA CAGCGTTCAG TCAGTGCCCC TCGCTGCTCC AGCTGATGAC GCCCCTGCAG 
AAGAATGCAT TGGTCATATA TTCTAAGCTG AGGCAGACAT GCAGAGAAGA CGGACACACG 
TACATTGAAG TAAAAGACTT GACCTCGGGC TTGTCGGAGC ATATGTCATT CGAGGAAGCG 
TGTCAGTCTC TGGCGTTTCT GAAGGACATT GACGTGGTCA TTTATGAGAA GGACTATGTC 
TTTCTTTCTG AACTGTACGA GGCCGAGCAA GACATCGCCT CCTCCATCTG CGAGCTGATG 
AGCAGACCTC CGTGGCACTT AAAAGTGGAC GTGAAGAACG TCCTGGCGTC AATTAGAGGC 
GCAAAGCCCA ACGATCCAGG AAGTGCTGAG GCAGTGGAAG GGAGCAAGCC GGAGGAGGTG 
GGGTCAGAGC AGGGTGACAG TGTTTTAGAC GCCCAAGATG GCGATGACCA TGTTAGAAGC 
AATGGCGAAC ACGTCGCAAA TGCAGAAATA AACGACGTCC CACTGGATCA AGATCAGGTG 
GTCGCCCTGG AGACGATCTG CGCCAACGCG GTGACTGTCC TCAGTGGGAA AGGCGGCTGT 
GGGAAGACCA CCATTGTTAG CCGTCTTTTT AAGCACATGG AACATCTGGA AGAAACGGAA 
GTGCAGCAAG CCTGCGAGGA TTTTGAACAA GACCAGGAAG CCTCAGAAGA GTGGCTTGAC 
TGTCCTAAGC AGAGCCCAGC CGGTGTGGAC AAGGCTGTAG AAGTCTTGCT CACCGCACCT 
ACAGGGAAGG CTGCTGGCTT ATTGAGGCAG AGGACCGATC TTCCGGCTTA CACGCTGTGC 
CAGGTCAATT ACAGCTTCTA TATGTGGAAA ACGAAAAATG AGGTGGACAA GCCGTGGAAA 
TTCTCCACGG TTCGAGTTCT GGTTGTGGAC GAAGGGAGTT TGGTGTCTGT AGGGATCTTC 
AAGTCAGTGT TACAGTTGCT GTGCAAACAC TCCAAGCTGT CCAAGCTCAT CATCCTTGGT 
GATGTTAGAC AGTTGCCCAG TATTGAACCT GGTAACATGC TGCAGGATGT CTTTGAGACT 
CTTAAGTCAA GACAGTGTGC CATCGAACTA AAGACGAACC ACAGGACAGA GTCTCAGCTG 
ATTGTGGACA ATGCTACAAG AATCTCCAGA CGTCAGTTTC CAAAATTTGA TGCTGAACTA 
AATATCTGTG GTAATCCTAC ATTACCTCTC TCCATTCAAG ATAAGACATT TATCTTTGTG 
AGACTCCCTG AAGAAGACAG CAGATCTCAG TCGTCTAAAG GTGAACACCG ATCTAATTTA 
TATACTGCAG TTAAAACTTT GCTTCAAGGA AAGGACTTCT GCTCTTTTGA AAGTTCAAAA 
ACATCGCAAT TTATTGCATT TAGAAGGCAA GACTGTGACC TCATCAATGA CTGCTGCTGC 
AAGCACTACA CAGGCCACCT CATCAAAGAC CATGAGAAGA AACTCATCTT TGCCGTCGGT 
GATAAGATCT GCTGTACCCG GAATGCATAC CTCTCAGATT TACTCCCCGA CAAAGACCAG 
GAAGCCGAGG GGAAAGGCTA TGGAGACGCC CCTGACGACG ATGCTAAAAT CAAACAGGAT 
TTCGAAAGCA GCACCCGCCT GTGCAACGGG GAAATATTTT TCATAACACG GGATGTAACT 
GACGTAACTT TCAAAAGAAA AAGACTTCTG ACCATTAATA ACGAGGCCGG TTTGGAAGTG 
ACTGTGGACT TTAGCAAACT AATGGCAAAC TGTCAGATCA AGCATGCCTG GGCAAGGACC 
ATTCATACTT TTCAGCTTGA CTCCAGCAGG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169270.1
CDS	154..2769
Translation	MARQDRLRELLGPLHPYKSDDEEEDCAQEEEGEQEEEFVDAEELCSGGIKAGSLPGRARVSIPDEYTKEKCTVYGRFPLKGPWWRVKVQVLKPQRSRSYQVQGFPAYFLQVDMSPPDQKQICSLFLKECNLASERIQEFLKWVEKVSSFENLHFENLWETLRLFYRETEKKDKKLSTPREQQGEEMRVEKSFAFISAMVALQFPKVMEFLPSLFPRHFKRLISSSSDWVLGCIEDVLGTQPWKLGFRRITYREMKLVRCEASWTAFSQCPSLLQLMTPLQKNALVIYSKLRQTCREDGHTYIEVKDLTSGLSEHMSFEEACQSLAFLKDIDVVIYEKDYVFLSELYEAEQDIASSICELMSRPPWHLKVDVKNVLASIRGAKPNDPGSAEAVEGSKPEEVGSEQGDSVLDAQDGDDHVRSNGEHVANAEINDVPLDQDQVVALETICANAVTVLSGKGGCGKTTIVSRLFKHMEHLEETEVQQACEDFEQDQEASEEWLDCPKQSPAGVDKAVEVLLTAPTGKAAGLLRQRTDLPAYTLCQVNYSFYMWKTKNEVDKPWKFSTVRVLVVDEGSLVSVGIFKSVLQLLCKHSKLSKLIILGDVRQLPSIEPGNMLQDVFETLKSRQCAIELKTNHRTESQLIVDNATRISRRQFPKFDAELNICGNPTLPLSIQDKTFIFVRLPEEDSRSQSSKGEHRSNLYTAVKTLLQGKDFCSFESSKTSQFIAFRRQDCDLINDCCCKHYTGHLIKDHEKKLIFAVGDKICCTRNAYLSDLLPDKDQEAEGKGYGDAPDDDAKIKQDFESSTRLCNGEIFFITRDVTDVTFKRKRLLTINNEAGLEVTVDFSKLMANCQIKHAWARTIHTFQLDSSRA

Target ORF information:

RefSeq Version	XM_017313781.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus helicase (DNA) B (Helb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313781.1

ORF Insert Sequence:

ATGGCTAGGC AGGATCGTCT GCGCGAGCTC CTGGGCCCTC TGCACCCGTA CAAGAGCGAC 
GACGAGGAGG AGGATTGTGC GCAGGAGGAG GAGGGGGAGC AGGAGGAGGA GTTTGTGGAT 
GCCGAGGAAC TCTGCAGCGG GGGCATCAAG GCGGGCAGCC TCCCTGGGCG CGCGCGAGTG 
TCTATCCCTG ATGAGTATAC TAAAGAGAAA TGTACCGTGT ACGGACGTTT CCCACTCAAA 
GGTCCGTGGT GGCGCGTGAA GGTCCAGGTC CTGAAACCCC AGCGATCCAG GAGCTATCAA 
GTTCAAGGCT TTCCGGCATA CTTTCTACAG GTTGATATGT CGCCACCAGA TCAAAAACAA 
ATCTGTTCGC TTTTTCTTAA AGAATGCAAC TTAGCCTCTG AGCGCATACA GGAATTTTTA 
AAATGGGTAG AAAAAGTGTC AAGCTTTGAA AATCTACACT TTGAAAATCT TTGGGAAACA 
TTAAGGCTTT TCTACAGAGA AACCGAAAAG AAAGATAAAA AGCTGTCTAC ACCAAGGGAG 
CAGCAGGGTG AGGAGATGAG AGTGGAAAAA TCATTCGCAT TTATAAGTGC AATGGTAGCT 
CTGCAATTCC CCAAAGTCAT GGAATTCCTT CCATCGCTTT TCCCTCGACA CTTCAAAAGG 
CTCATCAGCT CGAGTTCTGA TTGGGTGCTG GGATGCATAG AAGATGTTCT GGGTACACAG 
CCGTGGAAGC TGGGCTTCAG GAGGATAACC TACAGAGAAA TGAAACTAGT GCGCTGTGAG 
GCCAGCTGGA CAGCGTTCAG TCAGTGCCCC TCGCTGCTCC AGCTGATGAC GCCCCTGCAG 
AAGAATGCAT TGGTCATATA TTCTAAGCTG AGGCAGACAT GCAGAGAAGA CGGACACACG 
TACATTGAAG TAAAAGACTT GACCTCGGGC TTGTCGGAGC ATATGTCATT CGAGGAAGCG 
TGTCAGTCTC TGGCGTTTCT GAAGGACATT GACGTGGTCA TTTATGAGAA GGACTATGTC 
TTTCTTTCTG AACTGTACGA GGCCGAGCAA GACATCGCCT CCTCCATCTG CGAGCTGATG 
AGCAGACCTC CGTGGCACTT AAAAGTGGAC GTGAAGAACG TCCTGGCGTC AATTAGAGGC 
GCAAAGCCCA ACGATCCAGG AAGTGCTGAG GCAGTGGAAG GGAGCAAGCC GGAGGAGGTG 
GGGTCAGAGC AGGGTGACAG TGTTTTAGAC GCCCAAGATG GCGATGACCA TGTTAGAAGC 
AATGGCGAAC ACGTCGCAAA TGCAGAAATA AACGACGTCC CACTGGATCA AGATCAGGTG 
GTCGCCCTGG AGACGATCTG CGCCAACGCG GTGACTGTCC TCAGTGGGAA AGGCGGCTGT 
GGGAAGACCA CCATTGTTAG CCGTCTTTTT AAGCACATGG AACATCTGGA AGAAACGGAA 
GTGCAGCAAG CCTGCGAGGA TTTTGAACAA GACCAGGAAG CCTCAGAAGA GTGGCTTGAC 
TGTCCTAAGC AGAGCCCAGC CGGTGTGGAC AAGGCTGTAG AAGTCTTGCT CACCGCACCT 
ACAGGGAAGG CTGCTGGCTT ATTGAGGCAG AGGACCGATC TTCCGGCTTA CACGCTGTGC 
CAGGTCAATT ACAGCTTCTA TATGTGGAAA ACGAAAAATG AGGTGGACAA GCCGTGGAAA 
TTCTCCACGG TTCGAGTTCT GGTTGTGGAC GAAGGGAGTT TGGTGTCTGT AGGGATCTTC 
AAGTCAGTGT TACAGTTGCT GTGCAAACAC TCCAAGCTGT CCAAGCTCAT CATCCTTGGT 
GATGTTAGAC AGTTGCCCAG TATTGAACCT GGTAACATGC TGCAGGATGT CTTTGAGACT 
CTTAAGTCAA GACAGTGTGC CATCGAACTA AAGACGAACC ACAGGACAGA GTCTCAGCTG 
ATTGTGGACA ATGCTACAAG AATCTCCAGA CGTCAGTTTC CAAAATTTGA TGCTGAACTA 
AATATCTGTG GTAATCCTAC ATTACCTCTC TCCATTCAAG ATAAGACATT TATCTTTGTG 
AGACTCCCTG AAGAAGACAG CAGATCTCAG TCGTCTAAAG GTGAACACCG ATCTAATTTA 
TATACTGCAG TTAAAACTTT GCTTCAAGGA AAGGACTTCT GCTCTTTTGA AAGTTCAAAA 
ACATCGCAAT TTATTGCATT TAGAAGGCAA GACTGTGACC TCATCAATGA CTGCTGCTGC 
AAGCACTACA CAGGCCACCT CATCAAAGAC CATGAGAAGA AACTCATCTT TGCCGTCGGT 
GATAAGATCT GCTGTACCCG GAATGCATAC CTCTCAGATT TACTCCCCGA CAAAGACCAG 
GAAGCCGAGG GGAAAGGCTA TGGAGACGCC CCTGACGACG ATGCTAAAAT CAAACAGGAT 
TTCGAAAGCA GCACCCGCCT GTGCAACGGG GAAATATTTT TCATAACACG GGATGTAACT 
GACGTAACTT TCAAAAGAAA AAGACTTCTG ACCATTAATA ACGAGGCCGG TTTGGAAGTG 
ACTGTGGACT TTAGCAAACT AATGGCAAAC TGTCAGATCA AGCATGCCTG GGCAAGGACC 
ATTCATACTT TTCAGCTTGA CTCCAGCAGG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Helb ( NM_080446.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_536694.2 Mus musculus helicase (DNA) B (Helb), mRNA.

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CloneID	OMu09652
Clone ID Related Accession (Same CDS sequence)	NM_080446.2
Accession Version	NM_080446.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Mus musculus(house mouse)
Product	DNA helicase B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK131122.1 and AB048542.1. On Jan 18, 2008 this sequence version replaced NM_080446.1. ##Evidence-Data-START## Transcript exon combination :: AB048542.1, AK131122.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTAGGC AGGATCGTCT GCGCGAGCTC CTGGGCCCTC TGCACCCGTA CAAGAGCGAC 
GACGAGGAGG AGGATTGTGC GCAGGAGGAG GAGGGGGAGC AGGAGGAGGA GTTTGTGGAT 
GCCGAGGAAC TCTGCAGCGG GGGCATCAAG GCGGGCAGCC TCCCTGGGCG CGCGCGAGTG 
TCTATCCCTG ATGAGTATAC TAAAGAGAAA TGTACCGTGT ACGGACGTTT CCCACTCAAA 
GGTCCGTGGT GGCGCGTGAA GGTCCAGGTC CTGAAACCCC AGCGATCCAG GAGCTATCAA 
GTTCAAGGCT TTCCGGCATA CTTTCTACAG GTTGATATGT CGCCACCAGA TCAAAAACAA 
ATCTGTTCGC TTTTTCTTAA AGAATGCAAC TTAGCCTCTG AGCGCATACA GGAATTTTTA 
AAATGGGTAG AAAAAGTGTC AAGCTTTGAA AATCTACACT TTGAAAATCT TTGGGAAACA 
TTAAGGCTTT TCTACAGAGA AACCGAAAAG AAAGATAAAA AGCTGTCTAC ACCAAGGGAG 
CAGCAGGGTG AGGAGATGAG AGTGGAAAAA TCATTCGCAT TTATAAGTGC AATGGTAGCT 
CTGCAATTCC CCAAAGTCAT GGAATTCCTT CCATCGCTTT TCCCTCGACA CTTCAAAAGG 
CTCATCAGCT CGAGTTCTGA TTGGGTGCTG GGATGCATAG AAGATGTTCT GGGTACACAG 
CCGTGGAAGC TGGGCTTCAG GAGGATAACC TACAGAGAAA TGAAACTAGT GCGCTGTGAG 
GCCAGCTGGA CAGCGTTCAG TCAGTGCCCC TCGCTGCTCC AGCTGATGAC GCCCCTGCAG 
AAGAATGCAT TGGTCATATA TTCTAAGCTG AGGCAGACAT GCAGAGAAGA CGGACACACG 
TACATTGAAG TAAAAGACTT GACCTCGGGC TTGTCGGAGC ATATGTCATT CGAGGAAGCG 
TGTCAGTCTC TGGCGTTTCT GAAGGACATT GACGTGGTCA TTTATGAGAA GGACTATGTC 
TTTCTTTCTG AACTGTACGA GGCCGAGCAA GACATCGCCT CCTCCATCTG CGAGCTGATG 
AGCAGACCTC CGTGGCACTT AAAAGTGGAC GTGAAGAACG TCCTGGCGTC AATTAGAGGC 
GCAAAGCCCA ACGATCCAGG AAGTGCTGAG GCAGTGGAAG GGAGCAAGCC GGAGGAGGTG 
GGGTCAGAGC AGGGTGACAG TGTTTTAGAC GCCCAAGATG GCGATGACCA TGTTAGAAGC 
AATGGCGAAC ACGTCGCAAA TGCAGAAATA AACGACGTCC CACTGGATCA AGATCAGGTG 
GTCGCCCTGG AGACGATCTG CGCCAACGCG GTGACTGTCC TCAGTGGGAA AGGCGGCTGT 
GGGAAGACCA CCATTGTTAG CCGTCTTTTT AAGCACATGG AACATCTGGA AGAAACGGAA 
GTGCAGCAAG CCTGCGAGGA TTTTGAACAA GACCAGGAAG CCTCAGAAGA GTGGCTTGAC 
TGTCCTAAGC AGAGCCCAGC CGGTGTGGAC AAGGCTGTAG AAGTCTTGCT CACCGCACCT 
ACAGGGAAGG CTGCTGGCTT ATTGAGGCAG AGGACCGATC TTCCGGCTTA CACGCTGTGC 
CAGGTCAATT ACAGCTTCTA TATGTGGAAA ACGAAAAATG AGGTGGACAA GCCGTGGAAA 
TTCTCCACGG TTCGAGTTCT GGTTGTGGAC GAAGGGAGTT TGGTGTCTGT AGGGATCTTC 
AAGTCAGTGT TACAGTTGCT GTGCAAACAC TCCAAGCTGT CCAAGCTCAT CATCCTTGGT 
GATGTTAGAC AGTTGCCCAG TATTGAACCT GGTAACATGC TGCAGGATGT CTTTGAGACT 
CTTAAGTCAA GACAGTGTGC CATCGAACTA AAGACGAACC ACAGGACAGA GTCTCAGCTG 
ATTGTGGACA ATGCTACAAG AATCTCCAGA CGTCAGTTTC CAAAATTTGA TGCTGAACTA 
AATATCTGTG GTAATCCTAC ATTACCTCTC TCCATTCAAG ATAAGACATT TATCTTTGTG 
AGACTCCCTG AAGAAGACAG CAGATCTCAG TCGTCTAAAG GTGAACACCG ATCTAATTTA 
TATACTGCAG TTAAAACTTT GCTTCAAGGA AAGGACTTCT GCTCTTTTGA AAGTTCAAAA 
ACATCGCAAT TTATTGCATT TAGAAGGCAA GACTGTGACC TCATCAATGA CTGCTGCTGC 
AAGCACTACA CAGGCCACCT CATCAAAGAC CATGAGAAGA AACTCATCTT TGCCGTCGGT 
GATAAGATCT GCTGTACCCG GAATGCATAC CTCTCAGATT TACTCCCCGA CAAAGACCAG 
GAAGCCGAGG GGAAAGGCTA TGGAGACGCC CCTGACGACG ATGCTAAAAT CAAACAGGAT 
TTCGAAAGCA GCACCCGCCT GTGCAACGGG GAAATATTTT TCATAACACG GGATGTAACT 
GACGTAACTT TCAAAAGAAA AAGACTTCTG ACCATTAATA ACGAGGCCGG TTTGGAAGTG 
ACTGTGGACT TTAGCAAACT AATGGCAAAC TGTCAGATCA AGCATGCCTG GGCAAGGACC 
ATTCATACTT TTCAGGGGTC CGAGGAGAAC ACAGTCGTCT ACGTCGTGGG GAAGGCAGGC 
CGCCAGCACT GGCAACATGT TTACACGGCA GTGACCCGGG GCCGCTCCAG AGTGTACATC 
ATCGCGCAGG AGTCTGAACT CAGGAGTGCC ACCCGGAAGC GCGGTTTCCC CAGGCAGACG 
CGTTTGAAAC ACTTCTTGCA GAAGAAGCTC TCTGGCAGCT GCGCACCCTC AACAGGTTTC 
GCATCCCAGC CTTCGAGCCC TCGAGTCGGA GGAAGACCTG ACACCCAGCC TCCAGCGTCA 
CACCTCTGTA GAACCCCAGA CAACAAAGCC ACTGCAGACA GCGCCAGAGG CGACGAAAGA 
TGGCTGTCAG CTTCTGTTAA CGATGACGTG GATACGGATG AGGAGTCAGC ACAGCTCAGA 
GGGTCCAAAA GAATCGGTGA CGGCTTCCCG TTTGATGAAG AAAGCCCCAG TAAATTCCGT 
ATGGTTGAAG CGCCATCTCC ACAGGTGTCC TCTGTGTTTC AGAATATGAG GTTGAATACT 
CTCACTCCCA GACAGCTTTT TAAACCCACC GACAATCAAG ACACAGGTAC GGCAGGTGTG 
GCGGACGACG CCAACGATCC CAGCAATCAG GAGATGGAAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536694.2
CDS	104..3328
Translation	MARQDRLRELLGPLHPYKSDDEEEDCAQEEEGEQEEEFVDAEELCSGGIKAGSLPGRARVSIPDEYTKEKCTVYGRFPLKGPWWRVKVQVLKPQRSRSYQVQGFPAYFLQVDMSPPDQKQICSLFLKECNLASERIQEFLKWVEKVSSFENLHFENLWETLRLFYRETEKKDKKLSTPREQQGEEMRVEKSFAFISAMVALQFPKVMEFLPSLFPRHFKRLISSSSDWVLGCIEDVLGTQPWKLGFRRITYREMKLVRCEASWTAFSQCPSLLQLMTPLQKNALVIYSKLRQTCREDGHTYIEVKDLTSGLSEHMSFEEACQSLAFLKDIDVVIYEKDYVFLSELYEAEQDIASSICELMSRPPWHLKVDVKNVLASIRGAKPNDPGSAEAVEGSKPEEVGSEQGDSVLDAQDGDDHVRSNGEHVANAEINDVPLDQDQVVALETICANAVTVLSGKGGCGKTTIVSRLFKHMEHLEETEVQQACEDFEQDQEASEEWLDCPKQSPAGVDKAVEVLLTAPTGKAAGLLRQRTDLPAYTLCQVNYSFYMWKTKNEVDKPWKFSTVRVLVVDEGSLVSVGIFKSVLQLLCKHSKLSKLIILGDVRQLPSIEPGNMLQDVFETLKSRQCAIELKTNHRTESQLIVDNATRISRRQFPKFDAELNICGNPTLPLSIQDKTFIFVRLPEEDSRSQSSKGEHRSNLYTAVKTLLQGKDFCSFESSKTSQFIAFRRQDCDLINDCCCKHYTGHLIKDHEKKLIFAVGDKICCTRNAYLSDLLPDKDQEAEGKGYGDAPDDDAKIKQDFESSTRLCNGEIFFITRDVTDVTFKRKRLLTINNEAGLEVTVDFSKLMANCQIKHAWARTIHTFQGSEENTVVYVVGKAGRQHWQHVYTAVTRGRSRVYIIAQESELRSATRKRGFPRQTRLKHFLQKKLSGSCAPSTGFASQPSSPRVGGRPDTQPPASHLCRTPDNKATADSARGDERWLSASVNDDVDTDEESAQLRGSKRIGDGFPFDEESPSKFRMVEAPSPQVSSVFQNMRLNTLTPRQLFKPTDNQDTGTAGVADDANDPSNQEMEM

Target ORF information:

RefSeq Version	NM_080446.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus helicase (DNA) B (Helb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080446.2

ORF Insert Sequence:

ATGGCTAGGC AGGATCGTCT GCGCGAGCTC CTGGGCCCTC TGCACCCGTA CAAGAGCGAC 
GACGAGGAGG AGGATTGTGC GCAGGAGGAG GAGGGGGAGC AGGAGGAGGA GTTTGTGGAT 
GCCGAGGAAC TCTGCAGCGG GGGCATCAAG GCGGGCAGCC TCCCTGGGCG CGCGCGAGTG 
TCTATCCCTG ATGAGTATAC TAAAGAGAAA TGTACCGTGT ACGGACGTTT CCCACTCAAA 
GGTCCGTGGT GGCGCGTGAA GGTCCAGGTC CTGAAACCCC AGCGATCCAG GAGCTATCAA 
GTTCAAGGCT TTCCGGCATA CTTTCTACAG GTTGATATGT CGCCACCAGA TCAAAAACAA 
ATCTGTTCGC TTTTTCTTAA AGAATGCAAC TTAGCCTCTG AGCGCATACA GGAATTTTTA 
AAATGGGTAG AAAAAGTGTC AAGCTTTGAA AATCTACACT TTGAAAATCT TTGGGAAACA 
TTAAGGCTTT TCTACAGAGA AACCGAAAAG AAAGATAAAA AGCTGTCTAC ACCAAGGGAG 
CAGCAGGGTG AGGAGATGAG AGTGGAAAAA TCATTCGCAT TTATAAGTGC AATGGTAGCT 
CTGCAATTCC CCAAAGTCAT GGAATTCCTT CCATCGCTTT TCCCTCGACA CTTCAAAAGG 
CTCATCAGCT CGAGTTCTGA TTGGGTGCTG GGATGCATAG AAGATGTTCT GGGTACACAG 
CCGTGGAAGC TGGGCTTCAG GAGGATAACC TACAGAGAAA TGAAACTAGT GCGCTGTGAG 
GCCAGCTGGA CAGCGTTCAG TCAGTGCCCC TCGCTGCTCC AGCTGATGAC GCCCCTGCAG 
AAGAATGCAT TGGTCATATA TTCTAAGCTG AGGCAGACAT GCAGAGAAGA CGGACACACG 
TACATTGAAG TAAAAGACTT GACCTCGGGC TTGTCGGAGC ATATGTCATT CGAGGAAGCG 
TGTCAGTCTC TGGCGTTTCT GAAGGACATT GACGTGGTCA TTTATGAGAA GGACTATGTC 
TTTCTTTCTG AACTGTACGA GGCCGAGCAA GACATCGCCT CCTCCATCTG CGAGCTGATG 
AGCAGACCTC CGTGGCACTT AAAAGTGGAC GTGAAGAACG TCCTGGCGTC AATTAGAGGC 
GCAAAGCCCA ACGATCCAGG AAGTGCTGAG GCAGTGGAAG GGAGCAAGCC GGAGGAGGTG 
GGGTCAGAGC AGGGTGACAG TGTTTTAGAC GCCCAAGATG GCGATGACCA TGTTAGAAGC 
AATGGCGAAC ACGTCGCAAA TGCAGAAATA AACGACGTCC CACTGGATCA AGATCAGGTG 
GTCGCCCTGG AGACGATCTG CGCCAACGCG GTGACTGTCC TCAGTGGGAA AGGCGGCTGT 
GGGAAGACCA CCATTGTTAG CCGTCTTTTT AAGCACATGG AACATCTGGA AGAAACGGAA 
GTGCAGCAAG CCTGCGAGGA TTTTGAACAA GACCAGGAAG CCTCAGAAGA GTGGCTTGAC 
TGTCCTAAGC AGAGCCCAGC CGGTGTGGAC AAGGCTGTAG AAGTCTTGCT CACCGCACCT 
ACAGGGAAGG CTGCTGGCTT ATTGAGGCAG AGGACCGATC TTCCGGCTTA CACGCTGTGC 
CAGGTCAATT ACAGCTTCTA TATGTGGAAA ACGAAAAATG AGGTGGACAA GCCGTGGAAA 
TTCTCCACGG TTCGAGTTCT GGTTGTGGAC GAAGGGAGTT TGGTGTCTGT AGGGATCTTC 
AAGTCAGTGT TACAGTTGCT GTGCAAACAC TCCAAGCTGT CCAAGCTCAT CATCCTTGGT 
GATGTTAGAC AGTTGCCCAG TATTGAACCT GGTAACATGC TGCAGGATGT CTTTGAGACT 
CTTAAGTCAA GACAGTGTGC CATCGAACTA AAGACGAACC ACAGGACAGA GTCTCAGCTG 
ATTGTGGACA ATGCTACAAG AATCTCCAGA CGTCAGTTTC CAAAATTTGA TGCTGAACTA 
AATATCTGTG GTAATCCTAC ATTACCTCTC TCCATTCAAG ATAAGACATT TATCTTTGTG 
AGACTCCCTG AAGAAGACAG CAGATCTCAG TCGTCTAAAG GTGAACACCG ATCTAATTTA 
TATACTGCAG TTAAAACTTT GCTTCAAGGA AAGGACTTCT GCTCTTTTGA AAGTTCAAAA 
ACATCGCAAT TTATTGCATT TAGAAGGCAA GACTGTGACC TCATCAATGA CTGCTGCTGC 
AAGCACTACA CAGGCCACCT CATCAAAGAC CATGAGAAGA AACTCATCTT TGCCGTCGGT 
GATAAGATCT GCTGTACCCG GAATGCATAC CTCTCAGATT TACTCCCCGA CAAAGACCAG 
GAAGCCGAGG GGAAAGGCTA TGGAGACGCC CCTGACGACG ATGCTAAAAT CAAACAGGAT 
TTCGAAAGCA GCACCCGCCT GTGCAACGGG GAAATATTTT TCATAACACG GGATGTAACT 
GACGTAACTT TCAAAAGAAA AAGACTTCTG ACCATTAATA ACGAGGCCGG TTTGGAAGTG 
ACTGTGGACT TTAGCAAACT AATGGCAAAC TGTCAGATCA AGCATGCCTG GGCAAGGACC 
ATTCATACTT TTCAGGGGTC CGAGGAGAAC ACAGTCGTCT ACGTCGTGGG GAAGGCAGGC 
CGCCAGCACT GGCAACATGT TTACACGGCA GTGACCCGGG GCCGCTCCAG AGTGTACATC 
ATCGCGCAGG AGTCTGAACT CAGGAGTGCC ACCCGGAAGC GCGGTTTCCC CAGGCAGACG 
CGTTTGAAAC ACTTCTTGCA GAAGAAGCTC TCTGGCAGCT GCGCACCCTC AACAGGTTTC 
GCATCCCAGC CTTCGAGCCC TCGAGTCGGA GGAAGACCTG ACACCCAGCC TCCAGCGTCA 
CACCTCTGTA GAACCCCAGA CAACAAAGCC ACTGCAGACA GCGCCAGAGG CGACGAAAGA 
TGGCTGTCAG CTTCTGTTAA CGATGACGTG GATACGGATG AGGAGTCAGC ACAGCTCAGA 
GGGTCCAAAA GAATCGGTGA CGGCTTCCCG TTTGATGAAG AAAGCCCCAG TAAATTCCGT 
ATGGTTGAAG CGCCATCTCC ACAGGTGTCC TCTGTGTTTC AGAATATGAG GTTGAATACT 
CTCACTCCCA GACAGCTTTT TAAACCCACC GACAATCAAG ACACAGGTAC GGCAGGTGTG 
GCGGACGACG CCAACGATCC CAGCAATCAG GAGATGGAAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HELB Is a Feedback Inhibitor of DNA End Resection.
Molecular cell61(3)405-418(2016 Feb)
Tkáč J,Xu G,Adhikary H,Young JTF,Gallo D,Escribano-Díaz C,Krietsch J,Orthwein A,Munro M,Sol W,Al-Hakim A,Lin ZY,Jonkers J,Borst P,Brown GW,Gingras AC,Rottenberg S,Masson JY,Durocher D

Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDnas identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes11(2)127-35(2004 Apr)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Kitamura H,Nakagawa T,Nagase T,Ohara O,Koga H

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Molecular cloning of a cDNA encoding mouse DNA helicase B, which has homology to Escherichia coli RecD protein, and identification of a mutation in the DNA helicase B from tsFT848 temperature-sensitive DNA replication mutant cells.
Nucleic acids research29(18)3835-40(2001 Sep)
Tada S,Kobayashi T,Omori A,Kusa Y,Okumura N,Kodaira H,Ishimi Y,Seki M,Enomoto T

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Helb cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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