HELB cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	HELB
Entrez Gene ID	797342
Full Name	helicase (DNA) B
Synonyms	id:ibd3187,id:ibd5145,sb:cb596,si:dkey-150i15.3
General protein information	Preferred Names helicase (DNA) B Names DNA helicase B
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005164675.3	XP_005164732.1	DNA helicase B isoform X2
XM_001336741.6	XP_001336777.3	DNA helicase B
XM_001336741.5	XP_001336777.3	DNA helicase B isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HELB	XP_850757.2
Rattus norvegicus (Norway rat)	Helb	XP_006241488.1
Xenopus tropicalis (tropical clawed frog)	helb	XP_002937585.2
Homo sapiens (human)	HELB	NP_387467.2
Bos taurus (cattle)	HELB	NP_001193111.1
Danio rerio (zebrafish)	helb	XP_001336777.3
Gallus gallus (chicken)	HELB	XP_416077.3
Pan troglodytes (chimpanzee)	HELB	XP_001162205.1
Macaca mulatta (Rhesus monkey)	HELB	XP_001117092.1
Mus musculus (house mouse)	Helb	NP_536694.2

Gene Ontology

The following HELB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HELB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa09293	XM_005164675.3 Latest version!	Danio rerio helicase (DNA) B (helb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa09292	XM_001336741.6 Latest version!	Danio rerio helicase (DNA) B (helb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa09292	XM_001336741.5	Danio rerio helicase (DNA) B (helb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HELB ( XM_005164675.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164732.1 Danio rerio helicase (DNA) B (helb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa09293
Clone ID Related Accession (Same CDS sequence)	XM_005164675.3
Accession Version	XM_005164675.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	DNA helicase B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164675.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATATC TGCCAACTTT GCTGCCAGGC CAGTATCTGG ACATTATAAA AAGAGCAAGA 
ATAGAAGATA TTCCAGAAAC TTCAGCAATG GATGAAGACC CTCAAAACGA GCAGAGAAAA 
ACAAATGTAT TGTCCAAACT TGAGGAGCTA ATAAATACTA ATAATGTGTG GAAGCTGGGC 
TTCAACTATA TCATGTTTAA GGAATTGCAT CTGATCAGGT GTGAAGCAAA ACTTGAAGCC 
TTCAAGCTGT GCGATTTGTT TTCAGCTATC CCGCTTCTTC AGCGTAACGC GTTGATTTTA 
TATGCAGAAC TGAAGAAACA CTGCAGAGTC ACCGGAAGCA CTTACATAAC TCAGGAGATG 
CTTGTGGACA AAATATTTTC TGAAACCGGG GCGGATGGTG CCTGGGCAGC TCTGCACTTC 
CTTGTGACAC AGGGGGTGCT GATGAAGGAG AGCACCAAGG TTGCACTTCG AAACCTGCTG 
AAGTATGAGA AAGGAATTGC AGAATGTTTG ACAGGCCTGG TTCAAGGAGA CCCTTGGAAA 
ATCCACCTGG ATGTCAAGGA GGTTCTCCGT GAAGCTCAGA TAAAAAGATT AAGAGCAAAG 
GCTTGTGAAA AACACAATGC AACTATGAAA TCAAAGGATG ATGCAAACAC ATCTGCCTCC 
GCAGCAGAAA ATTCCACCGC TGTGTTTGAG CAGTTAGGCA TTGATTTAGA CCAGCCTGGT 
ACTTCAAGCA TGGTCCACAA TGGAGAAACA CCATCATGTG ATGATTCTGA AAATACAACG 
CTCACTATTA AAGAGGAAAA TCTCACATCA GACTCTGACT CAGATTTGGA CATTGATCCC 
AGTACGATAG AGTTGGATTG TGATCAGGTG CGGGCAGCCG AAATGATGTG TGCAAATCCG 
GTTACAGTGA TCAGTGGGAA AGGGGGTTGT GGGAAAACCA CAGTGGTCAG CCTGGTGTTT 
AAGGCGGCCA TGGAGCAGCA GACAAGCGAC TTGGAGGAGG TGCAGAAGGC CTGCGAGGAC 
TTTCAGAATG ACTCTCATGG CTCTAGTAAT GGGCTGGCGT TGGATGTGCA CGAGGAGAAA 
AACCATAGCG AGAAGAGCAT CAGTAATGAA AAATCTGTGG AAGTTCTCCT CACTGCTCCT 
ACAGGCAGAG CTGCTTCACT TCTCACTAAA AGAACTGGGT TCACTGCTTA CACCATGCAC 
CAGGTGTTAT GGAGTTTCAT GACTGTAGAT AAGGATCGAA GTGGGAATCC ACAGGACTGG 
AAGTTCTCAA AGGTTCGGGT GCTGGTGGTT GACGAGGGAA GCTTGGTTTC TGTTCAGATC 
CTTCACTCCA TTCTTAGCAT GCTTACCAAA CACGCACAAC TCCAGAAGTT CATCATTTTG 
GGTGATGTGC GGCAGTTGCC CAGCATTGAG CCTGGAAACA CACTATATGA TCTGTTTGAA 
GGTCTCAGAA GAGTCCGCTG GGCTATTGAG ATGAGGACCA ATCATCGAGC CGAGTCTGAG 
CTCATCGTCA GAAATGCTGG ACTCATCTCT GAGATGGGTA AAAAAAAGTA TTACAAGCCT 
CTGGACTTTG ATGCCACCAT AGACATGACA AACCCCTCTG CAGTGCCATC CGACAAAAGA 
TTCATTTTTA TCAAAATCAG TTCGGACAAT TTTTCTTTTG ACCTCCAGGA AGCCATCTTG 
TTCCTACTTA AGGAAGCTCC TGGGCTGAAA GATCATATAT TGTCACAGTT TGTGGCTTTT 
CGGAGGAAGG ATTGTGAGTT AATCAATGAG CTTTGCTGCA GACATTATTC AAACCATACC 
ACAAAAACTG CCAAGAAAAC ATTCAACTTT CAGGTAGGTG ATAAAGTCTG CTGCACTAAG 
AATGGCTACG TCACAGACTA TGATAAGAAG GAGACGTCTT TGGCGGACAT CAGAGCTTCA 
CATAATCGTG CTGAAAGTCA ACAAGATGAT CAGAAGAAAG AAAAGAAAGA GAAGAAAGAG 
CGATTGTGCA ATGGCGAAAT TTTCTTCATT AAAGATGATG TGACTAAATC TGTCGAGAAT 
GAAAAAAATA AAATGGTGCG TCAACTGACA CTCGATGATA AAAGCGACCG CCAGGTGACT 
TGTAACTACA AAGAGCTACA GAGAGAGTGT AAACTGCGCC ATTCTTGGGC AAGAACCATT 
CACACCTTTC AGGGCTCAGA AGCTGAAACG ATTGTATACA TTCTTGGAGA CAGTCCTGCC 
CAAAACTGGC AGCATGTTTA CACCGCGGTG ACTCGTGGTC AGAAGCGTGT GTATGTGGTG 
GGTACAGAGT ATGATCTAGA AGGTGCCATC AGGAAGAGGA TCACCCCTCG TAACACACGA 
CTCTGCGGCT TTGTCAACAC TAAAGTTGGC CAACAAGACC CTTTGACTAC ATCTGCCTCC 
ACCCAGAGGC AAGCAGAGAC TACCTTGAAC CACAGTTTTG GGCATTCACA ATCAACCCCA 
GTGGCCTCGC AAACTCCAAT CCGCCCTCAG ATGTTGTCCA GTACACGACC TTCATGTGCA 
AGAAACCTCT ACACTGACAA AAATGGAGAA GGCGATCAAA CGTCCAATAT CTCTTTGCAA 
GATGACATGG CATTCAGTCA AGCCTACTCG TGGTCACCCA TGGACAGCTG CGATGAGGCT 
GGCAGGGTGC AGAATGAAAA TATGACCGAA TTATCTAATC ATGTTGATGA TGCTTTGTTG 
GCAACGCTCA GCTCATCCCC AAATGAAAGC AGCAGGGGAT CAAAACGCAC GATTGCTGAA 
GACATCTACA GTACGCCATC TAAACTACCA AAGCAAACTG CTTCAGAAGA GTCTCCAATG 
GTCAGCTCAA GGCTGAATCT TCTGTCCCTT TCCAGTCCTG ACATCAAATC CAGCCGCCAG 
CTCTTCCAAG ACGATACACA CTCTCACAGG GGTCAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164732.1
CDS	716..3637
Translation	MKYLPTLLPGQYLDIIKRARIEDIPETSAMDEDPQNEQRKTNVLSKLEELINTNNVWKLGFNYIMFKELHLIRCEAKLEAFKLCDLFSAIPLLQRNALILYAELKKHCRVTGSTYITQEMLVDKIFSETGADGAWAALHFLVTQGVLMKESTKVALRNLLKYEKGIAECLTGLVQGDPWKIHLDVKEVLREAQIKRLRAKACEKHNATMKSKDDANTSASAAENSTAVFEQLGIDLDQPGTSSMVHNGETPSCDDSENTTLTIKEENLTSDSDSDLDIDPSTIELDCDQVRAAEMMCANPVTVISGKGGCGKTTVVSLVFKAAMEQQTSDLEEVQKACEDFQNDSHGSSNGLALDVHEEKNHSEKSISNEKSVEVLLTAPTGRAASLLTKRTGFTAYTMHQVLWSFMTVDKDRSGNPQDWKFSKVRVLVVDEGSLVSVQILHSILSMLTKHAQLQKFIILGDVRQLPSIEPGNTLYDLFEGLRRVRWAIEMRTNHRAESELIVRNAGLISEMGKKKYYKPLDFDATIDMTNPSAVPSDKRFIFIKISSDNFSFDLQEAILFLLKEAPGLKDHILSQFVAFRRKDCELINELCCRHYSNHTTKTAKKTFNFQVGDKVCCTKNGYVTDYDKKETSLADIRASHNRAESQQDDQKKEKKEKKERLCNGEIFFIKDDVTKSVENEKNKMVRQLTLDDKSDRQVTCNYKELQRECKLRHSWARTIHTFQGSEAETIVYILGDSPAQNWQHVYTAVTRGQKRVYVVGTEYDLEGAIRKRITPRNTRLCGFVNTKVGQQDPLTTSASTQRQAETTLNHSFGHSQSTPVASQTPIRPQMLSSTRPSCARNLYTDKNGEGDQTSNISLQDDMAFSQAYSWSPMDSCDEAGRVQNENMTELSNHVDDALLATLSSSPNESSRGSKRTIAEDIYSTPSKLPKQTASEESPMVSSRLNLLSLSSPDIKSSRQLFQDDTHSHRGQP

Target ORF information:

RefSeq Version	XM_005164675.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio helicase (DNA) B (helb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164675.3

ORF Insert Sequence:

ATGAAATATC TGCCAACTTT GCTGCCAGGC CAGTATCTGG ACATTATAAA AAGAGCAAGA 
ATAGAAGATA TTCCAGAAAC TTCAGCAATG GATGAAGACC CTCAAAACGA GCAGAGAAAA 
ACAAATGTAT TGTCCAAACT TGAGGAGCTA ATAAATACTA ATAATGTGTG GAAGCTGGGC 
TTCAACTATA TCATGTTTAA GGAATTGCAT CTGATCAGGT GTGAAGCAAA ACTTGAAGCC 
TTCAAGCTGT GCGATTTGTT TTCAGCTATC CCGCTTCTTC AGCGTAACGC GTTGATTTTA 
TATGCAGAAC TGAAGAAACA CTGCAGAGTC ACCGGAAGCA CTTACATAAC TCAGGAGATG 
CTTGTGGACA AAATATTTTC TGAAACCGGG GCGGATGGTG CCTGGGCAGC TCTGCACTTC 
CTTGTGACAC AGGGGGTGCT GATGAAGGAG AGCACCAAGG TTGCACTTCG AAACCTGCTG 
AAGTATGAGA AAGGAATTGC AGAATGTTTG ACAGGCCTGG TTCAAGGAGA CCCTTGGAAA 
ATCCACCTGG ATGTCAAGGA GGTTCTCCGT GAAGCTCAGA TAAAAAGATT AAGAGCAAAG 
GCTTGTGAAA AACACAATGC AACTATGAAA TCAAAGGATG ATGCAAACAC ATCTGCCTCC 
GCAGCAGAAA ATTCCACCGC TGTGTTTGAG CAGTTAGGCA TTGATTTAGA CCAGCCTGGT 
ACTTCAAGCA TGGTCCACAA TGGAGAAACA CCATCATGTG ATGATTCTGA AAATACAACG 
CTCACTATTA AAGAGGAAAA TCTCACATCA GACTCTGACT CAGATTTGGA CATTGATCCC 
AGTACGATAG AGTTGGATTG TGATCAGGTG CGGGCAGCCG AAATGATGTG TGCAAATCCG 
GTTACAGTGA TCAGTGGGAA AGGGGGTTGT GGGAAAACCA CAGTGGTCAG CCTGGTGTTT 
AAGGCGGCCA TGGAGCAGCA GACAAGCGAC TTGGAGGAGG TGCAGAAGGC CTGCGAGGAC 
TTTCAGAATG ACTCTCATGG CTCTAGTAAT GGGCTGGCGT TGGATGTGCA CGAGGAGAAA 
AACCATAGCG AGAAGAGCAT CAGTAATGAA AAATCTGTGG AAGTTCTCCT CACTGCTCCT 
ACAGGCAGAG CTGCTTCACT TCTCACTAAA AGAACTGGGT TCACTGCTTA CACCATGCAC 
CAGGTGTTAT GGAGTTTCAT GACTGTAGAT AAGGATCGAA GTGGGAATCC ACAGGACTGG 
AAGTTCTCAA AGGTTCGGGT GCTGGTGGTT GACGAGGGAA GCTTGGTTTC TGTTCAGATC 
CTTCACTCCA TTCTTAGCAT GCTTACCAAA CACGCACAAC TCCAGAAGTT CATCATTTTG 
GGTGATGTGC GGCAGTTGCC CAGCATTGAG CCTGGAAACA CACTATATGA TCTGTTTGAA 
GGTCTCAGAA GAGTCCGCTG GGCTATTGAG ATGAGGACCA ATCATCGAGC CGAGTCTGAG 
CTCATCGTCA GAAATGCTGG ACTCATCTCT GAGATGGGTA AAAAAAAGTA TTACAAGCCT 
CTGGACTTTG ATGCCACCAT AGACATGACA AACCCCTCTG CAGTGCCATC CGACAAAAGA 
TTCATTTTTA TCAAAATCAG TTCGGACAAT TTTTCTTTTG ACCTCCAGGA AGCCATCTTG 
TTCCTACTTA AGGAAGCTCC TGGGCTGAAA GATCATATAT TGTCACAGTT TGTGGCTTTT 
CGGAGGAAGG ATTGTGAGTT AATCAATGAG CTTTGCTGCA GACATTATTC AAACCATACC 
ACAAAAACTG CCAAGAAAAC ATTCAACTTT CAGGTAGGTG ATAAAGTCTG CTGCACTAAG 
AATGGCTACG TCACAGACTA TGATAAGAAG GAGACGTCTT TGGCGGACAT CAGAGCTTCA 
CATAATCGTG CTGAAAGTCA ACAAGATGAT CAGAAGAAAG AAAAGAAAGA GAAGAAAGAG 
CGATTGTGCA ATGGCGAAAT TTTCTTCATT AAAGATGATG TGACTAAATC TGTCGAGAAT 
GAAAAAAATA AAATGGTGCG TCAACTGACA CTCGATGATA AAAGCGACCG CCAGGTGACT 
TGTAACTACA AAGAGCTACA GAGAGAGTGT AAACTGCGCC ATTCTTGGGC AAGAACCATT 
CACACCTTTC AGGGCTCAGA AGCTGAAACG ATTGTATACA TTCTTGGAGA CAGTCCTGCC 
CAAAACTGGC AGCATGTTTA CACCGCGGTG ACTCGTGGTC AGAAGCGTGT GTATGTGGTG 
GGTACAGAGT ATGATCTAGA AGGTGCCATC AGGAAGAGGA TCACCCCTCG TAACACACGA 
CTCTGCGGCT TTGTCAACAC TAAAGTTGGC CAACAAGACC CTTTGACTAC ATCTGCCTCC 
ACCCAGAGGC AAGCAGAGAC TACCTTGAAC CACAGTTTTG GGCATTCACA ATCAACCCCA 
GTGGCCTCGC AAACTCCAAT CCGCCCTCAG ATGTTGTCCA GTACACGACC TTCATGTGCA 
AGAAACCTCT ACACTGACAA AAATGGAGAA GGCGATCAAA CGTCCAATAT CTCTTTGCAA 
GATGACATGG CATTCAGTCA AGCCTACTCG TGGTCACCCA TGGACAGCTG CGATGAGGCT 
GGCAGGGTGC AGAATGAAAA TATGACCGAA TTATCTAATC ATGTTGATGA TGCTTTGTTG 
GCAACGCTCA GCTCATCCCC AAATGAAAGC AGCAGGGGAT CAAAACGCAC GATTGCTGAA 
GACATCTACA GTACGCCATC TAAACTACCA AAGCAAACTG CTTCAGAAGA GTCTCCAATG 
GTCAGCTCAA GGCTGAATCT TCTGTCCCTT TCCAGTCCTG ACATCAAATC CAGCCGCCAG 
CTCTTCCAAG ACGATACACA CTCTCACAGG GGTCAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELB ( XM_001336741.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001336777.3 Danio rerio helicase (DNA) B (helb), mRNA.

Price & Availability↑

CloneID	ODa09292
Clone ID Related Accession (Same CDS sequence)	XM_001336741.6 , XM_001336741.5
Accession Version	XM_001336741.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	DNA helicase B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001336741.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTTC AAAGTCCCAT TGCGGGATTA AAAACCATAA CTGGTTACAT TTTACCAGAA 
GAACAAGCTG TCCAGCCAAA CAAAGACGAT GGTTTAGAAA GTGAAGATGA AGAGGAGGAA 
GGACAGCCTG AATTTCTGGA CATGAAGGAG ATGAACTCGG TGTCATCTGG GGGAAACATG 
TTCAAATCTT TAGTGCCAAC ATCAAAAGAA GTGTCAATTC TGGTTGGTAA AAAGACATAC 
AAGGTTGAGG GCCGATTCCC CCTCTGTGAC CCCTGGTGGG AAGTAACCTG TACAATTCGC 
CTAGGCCGAC TCAAGGCATT TGTTAAAGGC TACCCTTTCT ATCGACTCCG CACTCATCTG 
AAATCAGAGG GTCGGTCACT CTTGTCTCTT TTTCTCACCG CATGTAAGGC ACAAGCTGAT 
CATGTTAAAA TGTTTATGGA CTGGTTGCCC ACTGACAGAC GTGTGGAGCT TGTTAATGTG 
CTGGACGTCC TGCAGGAGTT TGAAGATTCC TCACTTGAGC ATGAAGCTGT GGCTCAACAG 
CTCAAGTCAA GTGTCAATTG TTCAGTTGTT TGGACGCACG TAAAAGCTGC CAGCTTGTAC 
CCTGGAATCA TGAAATATCT GCCAACTTTG CTGCCAGGCC AGTATCTGGA CATTATAAAA 
AGAGCAAGAA TAGAAGATAT TCCAGAAACT TCAGCAATGG ATGAAGACCC TCAAAACGAG 
CAGAGAAAAA CAAATGTATT GTCCAAACTT GAGGAGCTAA TAAATACTAA TAATGTGTGG 
AAGCTGGGCT TCAACTATAT CATGTTTAAG GAATTGCATC TGATCAGGTG TGAAGCAAAA 
CTTGAAGCCT TCAAGCTGTG CGATTTGTTT TCAGCTATCC CGCTTCTTCA GCGTAACGCG 
TTGATTTTAT ATGCAGAACT GAAGAAACAC TGCAGAGTCA CCGGAAGCAC TTACATAACT 
CAGGAGATGC TTGTGGACAA AATATTTTCT GAAACCGGGG CGGATGGTGC CTGGGCAGCT 
CTGCACTTCC TTGTGACACA GGGGGTGCTG ATGAAGGAGA GCACCAAGGT TGCACTTCGA 
AACCTGCTGA AGTATGAGAA AGGAATTGCA GAATGTTTGA CAGGCCTGGT TCAAGGAGAC 
CCTTGGAAAA TCCACCTGGA TGTCAAGGAG GTTCTCCGTG AAGCTCAGAT AAAAAGATTA 
AGAGCAAAGG CTTGTGAAAA ACACAATGCA ACTATGAAAT CAAAGGATGA TGCAAACACA 
TCTGCCTCCG CAGCAGAAAA TTCCACCGCT GTGTTTGAGC AGTTAGGCAT TGATTTAGAC 
CAGCCTGGTA CTTCAAGCAT GGTCCACAAT GGAGAAACAC CATCATGTGA TGATTCTGAA 
AATACAACGC TCACTATTAA AGAGGAAAAT CTCACATCAG ACTCTGACTC AGATTTGGAC 
ATTGATCCCA GTACGATAGA GTTGGATTGT GATCAGGTGC GGGCAGCCGA AATGATGTGT 
GCAAATCCGG TTACAGTGAT CAGTGGGAAA GGGGGTTGTG GGAAAACCAC AGTGGTCAGC 
CTGGTGTTTA AGGCGGCCAT GGAGCAGCAG ACAAGCGACT TGGAGGAGGT GCAGAAGGCC 
TGCGAGGACT TTCAGAATGA CTCTCATGGC TCTAGTAATG GGCTGGCGTT GGATGTGCAC 
GAGGAGAAAA ACCATAGCGA GAAGAGCATC AGTAATGAAA AATCTGTGGA AGTTCTCCTC 
ACTGCTCCTA CAGGCAGAGC TGCTTCACTT CTCACTAAAA GAACTGGGTT CACTGCTTAC 
ACCATGCACC AGGTGTTATG GAGTTTCATG ACTGTAGATA AGGATCGAAG TGGGAATCCA 
CAGGACTGGA AGTTCTCAAA GGTTCGGGTG CTGGTGGTTG ACGAGGGAAG CTTGGTTTCT 
GTTCAGATCC TTCACTCCAT TCTTAGCATG CTTACCAAAC ACGCACAACT CCAGAAGTTC 
ATCATTTTGG GTGATGTGCG GCAGTTGCCC AGCATTGAGC CTGGAAACAC ACTATATGAT 
CTGTTTGAAG GTCTCAGAAG AGTCCGCTGG GCTATTGAGA TGAGGACCAA TCATCGAGCC 
GAGTCTGAGC TCATCGTCAG AAATGCTGGA CTCATCTCTG AGATGGGTAA AAAAAAGTAT 
TACAAGCCTC TGGACTTTGA TGCCACCATA GACATGACAA ACCCCTCTGC AGTGCCATCC 
GACAAAAGAT TCATTTTTAT CAAAATCAGT TCGGACAATT TTTCTTTTGA CCTCCAGGAA 
GCCATCTTGT TCCTACTTAA GGAAGCTCCT GGGCTGAAAG ATCATATATT GTCACAGTTT 
GTGGCTTTTC GGAGGAAGGA TTGTGAGTTA ATCAATGAGC TTTGCTGCAG ACATTATTCA 
AACCATACCA CAAAAACTGC CAAGAAAACA TTCAACTTTC AGGTAGGTGA TAAAGTCTGC 
TGCACTAAGA ATGGCTACGT CACAGACTAT GATAAGAAGG AGACGTCTTT GGCGGACATC 
AGAGCTTCAC ATAATCGTGC TGAAAGTCAA CAAGATGATC AGAAGAAAGA AAAGAAAGAG 
AAGAAAGAGC GATTGTGCAA TGGCGAAATT TTCTTCATTA AAGATGATGT GACTAAATCT 
GTCGAGAATG AAAAAAATAA AATGGTGCGT CAACTGACAC TCGATGATAA AAGCGACCGC 
CAGGTGACTT GTAACTACAA AGAGCTACAG AGAGAGTGTA AACTGCGCCA TTCTTGGGCA 
AGAACCATTC ACACCTTTCA GGGCTCAGAA GCTGAAACGA TTGTATACAT TCTTGGAGAC 
AGTCCTGCCC AAAACTGGCA GCATGTTTAC ACCGCGGTGA CTCGTGGTCA GAAGCGTGTG 
TATGTGGTGG GTACAGAGTA TGATCTAGAA GGTGCCATCA GGAAGAGGAT CACCCCTCGT 
AACACACGAC TCTGCGGCTT TGTCAACACT AAAGTTGGCC AACAAGACCC TTTGACTACA 
TCTGCCTCCA CCCAGAGGCA AGCAGAGACT ACCTTGAACC ACAGTTTTGG GCATTCACAA 
TCAACCCCAG TGGCCTCGCA AACTCCAATC CGCCCTCAGA TGTTGTCCAG TACACGACCT 
TCATGTGCAA GAAACCTCTA CACTGACAAA AATGGAGAAG GCGATCAAAC GTCCAATATC 
TCTTTGCAAG ATGACATGGC ATTCAGTCAA GCCTACTCGT GGTCACCCAT GGACAGCTGC 
GATGAGGCTG GCAGGGTGCA GAATGAAAAT ATGACCGAAT TATCTAATCA TGTTGATGAT 
GCTTTGTTGG CAACGCTCAG CTCATCCCCA AATGAAAGCA GCAGGGGATC AAAACGCACG 
ATTGCTGAAG ACATCTACAG TACGCCATCT AAACTACCAA AGCAAACTGC TTCAGAAGAG 
TCTCCAATGG TCAGCTCAAG GCTGAATCTT CTGTCCCTTT CCAGTCCTGA CATCAAATCC 
AGCCGCCAGC TCTTCCAAGA CGATACACAC TCTCACAGGG GTCAGCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001336777.3
CDS	95..3625
Translation	MALQSPIAGLKTITGYILPEEQAVQPNKDDGLESEDEEEEGQPEFLDMKEMNSVSSGGNMFKSLVPTSKEVSILVGKKTYKVEGRFPLCDPWWEVTCTIRLGRLKAFVKGYPFYRLRTHLKSEGRSLLSLFLTACKAQADHVKMFMDWLPTDRRVELVNVLDVLQEFEDSSLEHEAVAQQLKSSVNCSVVWTHVKAASLYPGIMKYLPTLLPGQYLDIIKRARIEDIPETSAMDEDPQNEQRKTNVLSKLEELINTNNVWKLGFNYIMFKELHLIRCEAKLEAFKLCDLFSAIPLLQRNALILYAELKKHCRVTGSTYITQEMLVDKIFSETGADGAWAALHFLVTQGVLMKESTKVALRNLLKYEKGIAECLTGLVQGDPWKIHLDVKEVLREAQIKRLRAKACEKHNATMKSKDDANTSASAAENSTAVFEQLGIDLDQPGTSSMVHNGETPSCDDSENTTLTIKEENLTSDSDSDLDIDPSTIELDCDQVRAAEMMCANPVTVISGKGGCGKTTVVSLVFKAAMEQQTSDLEEVQKACEDFQNDSHGSSNGLALDVHEEKNHSEKSISNEKSVEVLLTAPTGRAASLLTKRTGFTAYTMHQVLWSFMTVDKDRSGNPQDWKFSKVRVLVVDEGSLVSVQILHSILSMLTKHAQLQKFIILGDVRQLPSIEPGNTLYDLFEGLRRVRWAIEMRTNHRAESELIVRNAGLISEMGKKKYYKPLDFDATIDMTNPSAVPSDKRFIFIKISSDNFSFDLQEAILFLLKEAPGLKDHILSQFVAFRRKDCELINELCCRHYSNHTTKTAKKTFNFQVGDKVCCTKNGYVTDYDKKETSLADIRASHNRAESQQDDQKKEKKEKKERLCNGEIFFIKDDVTKSVENEKNKMVRQLTLDDKSDRQVTCNYKELQRECKLRHSWARTIHTFQGSEAETIVYILGDSPAQNWQHVYTAVTRGQKRVYVVGTEYDLEGAIRKRITPRNTRLCGFVNTKVGQQDPLTTSASTQRQAETTLNHSFGHSQSTPVASQTPIRPQMLSSTRPSCARNLYTDKNGEGDQTSNISLQDDMAFSQAYSWSPMDSCDEAGRVQNENMTELSNHVDDALLATLSSSPNESSRGSKRTIAEDIYSTPSKLPKQTASEESPMVSSRLNLLSLSSPDIKSSRQLFQDDTHSHRGQP

Target ORF information:

RefSeq Version	XM_001336741.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio helicase (DNA) B (helb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001336741.6

ORF Insert Sequence:

ATGGCGCTTC AAAGTCCCAT TGCGGGATTA AAAACCATAA CTGGTTACAT TTTACCAGAA 
GAACAAGCTG TCCAGCCAAA CAAAGACGAT GGTTTAGAAA GTGAAGATGA AGAGGAGGAA 
GGACAGCCTG AATTTCTGGA CATGAAGGAG ATGAACTCGG TGTCATCTGG GGGAAACATG 
TTCAAATCTT TAGTGCCAAC ATCAAAAGAA GTGTCAATTC TGGTTGGTAA AAAGACATAC 
AAGGTTGAGG GCCGATTCCC CCTCTGTGAC CCCTGGTGGG AAGTAACCTG TACAATTCGC 
CTAGGCCGAC TCAAGGCATT TGTTAAAGGC TACCCTTTCT ATCGACTCCG CACTCATCTG 
AAATCAGAGG GTCGGTCACT CTTGTCTCTT TTTCTCACCG CATGTAAGGC ACAAGCTGAT 
CATGTTAAAA TGTTTATGGA CTGGTTGCCC ACTGACAGAC GTGTGGAGCT TGTTAATGTG 
CTGGACGTCC TGCAGGAGTT TGAAGATTCC TCACTTGAGC ATGAAGCTGT GGCTCAACAG 
CTCAAGTCAA GTGTCAATTG TTCAGTTGTT TGGACGCACG TAAAAGCTGC CAGCTTGTAC 
CCTGGAATCA TGAAATATCT GCCAACTTTG CTGCCAGGCC AGTATCTGGA CATTATAAAA 
AGAGCAAGAA TAGAAGATAT TCCAGAAACT TCAGCAATGG ATGAAGACCC TCAAAACGAG 
CAGAGAAAAA CAAATGTATT GTCCAAACTT GAGGAGCTAA TAAATACTAA TAATGTGTGG 
AAGCTGGGCT TCAACTATAT CATGTTTAAG GAATTGCATC TGATCAGGTG TGAAGCAAAA 
CTTGAAGCCT TCAAGCTGTG CGATTTGTTT TCAGCTATCC CGCTTCTTCA GCGTAACGCG 
TTGATTTTAT ATGCAGAACT GAAGAAACAC TGCAGAGTCA CCGGAAGCAC TTACATAACT 
CAGGAGATGC TTGTGGACAA AATATTTTCT GAAACCGGGG CGGATGGTGC CTGGGCAGCT 
CTGCACTTCC TTGTGACACA GGGGGTGCTG ATGAAGGAGA GCACCAAGGT TGCACTTCGA 
AACCTGCTGA AGTATGAGAA AGGAATTGCA GAATGTTTGA CAGGCCTGGT TCAAGGAGAC 
CCTTGGAAAA TCCACCTGGA TGTCAAGGAG GTTCTCCGTG AAGCTCAGAT AAAAAGATTA 
AGAGCAAAGG CTTGTGAAAA ACACAATGCA ACTATGAAAT CAAAGGATGA TGCAAACACA 
TCTGCCTCCG CAGCAGAAAA TTCCACCGCT GTGTTTGAGC AGTTAGGCAT TGATTTAGAC 
CAGCCTGGTA CTTCAAGCAT GGTCCACAAT GGAGAAACAC CATCATGTGA TGATTCTGAA 
AATACAACGC TCACTATTAA AGAGGAAAAT CTCACATCAG ACTCTGACTC AGATTTGGAC 
ATTGATCCCA GTACGATAGA GTTGGATTGT GATCAGGTGC GGGCAGCCGA AATGATGTGT 
GCAAATCCGG TTACAGTGAT CAGTGGGAAA GGGGGTTGTG GGAAAACCAC AGTGGTCAGC 
CTGGTGTTTA AGGCGGCCAT GGAGCAGCAG ACAAGCGACT TGGAGGAGGT GCAGAAGGCC 
TGCGAGGACT TTCAGAATGA CTCTCATGGC TCTAGTAATG GGCTGGCGTT GGATGTGCAC 
GAGGAGAAAA ACCATAGCGA GAAGAGCATC AGTAATGAAA AATCTGTGGA AGTTCTCCTC 
ACTGCTCCTA CAGGCAGAGC TGCTTCACTT CTCACTAAAA GAACTGGGTT CACTGCTTAC 
ACCATGCACC AGGTGTTATG GAGTTTCATG ACTGTAGATA AGGATCGAAG TGGGAATCCA 
CAGGACTGGA AGTTCTCAAA GGTTCGGGTG CTGGTGGTTG ACGAGGGAAG CTTGGTTTCT 
GTTCAGATCC TTCACTCCAT TCTTAGCATG CTTACCAAAC ACGCACAACT CCAGAAGTTC 
ATCATTTTGG GTGATGTGCG GCAGTTGCCC AGCATTGAGC CTGGAAACAC ACTATATGAT 
CTGTTTGAAG GTCTCAGAAG AGTCCGCTGG GCTATTGAGA TGAGGACCAA TCATCGAGCC 
GAGTCTGAGC TCATCGTCAG AAATGCTGGA CTCATCTCTG AGATGGGTAA AAAAAAGTAT 
TACAAGCCTC TGGACTTTGA TGCCACCATA GACATGACAA ACCCCTCTGC AGTGCCATCC 
GACAAAAGAT TCATTTTTAT CAAAATCAGT TCGGACAATT TTTCTTTTGA CCTCCAGGAA 
GCCATCTTGT TCCTACTTAA GGAAGCTCCT GGGCTGAAAG ATCATATATT GTCACAGTTT 
GTGGCTTTTC GGAGGAAGGA TTGTGAGTTA ATCAATGAGC TTTGCTGCAG ACATTATTCA 
AACCATACCA CAAAAACTGC CAAGAAAACA TTCAACTTTC AGGTAGGTGA TAAAGTCTGC 
TGCACTAAGA ATGGCTACGT CACAGACTAT GATAAGAAGG AGACGTCTTT GGCGGACATC 
AGAGCTTCAC ATAATCGTGC TGAAAGTCAA CAAGATGATC AGAAGAAAGA AAAGAAAGAG 
AAGAAAGAGC GATTGTGCAA TGGCGAAATT TTCTTCATTA AAGATGATGT GACTAAATCT 
GTCGAGAATG AAAAAAATAA AATGGTGCGT CAACTGACAC TCGATGATAA AAGCGACCGC 
CAGGTGACTT GTAACTACAA AGAGCTACAG AGAGAGTGTA AACTGCGCCA TTCTTGGGCA 
AGAACCATTC ACACCTTTCA GGGCTCAGAA GCTGAAACGA TTGTATACAT TCTTGGAGAC 
AGTCCTGCCC AAAACTGGCA GCATGTTTAC ACCGCGGTGA CTCGTGGTCA GAAGCGTGTG 
TATGTGGTGG GTACAGAGTA TGATCTAGAA GGTGCCATCA GGAAGAGGAT CACCCCTCGT 
AACACACGAC TCTGCGGCTT TGTCAACACT AAAGTTGGCC AACAAGACCC TTTGACTACA 
TCTGCCTCCA CCCAGAGGCA AGCAGAGACT ACCTTGAACC ACAGTTTTGG GCATTCACAA 
TCAACCCCAG TGGCCTCGCA AACTCCAATC CGCCCTCAGA TGTTGTCCAG TACACGACCT 
TCATGTGCAA GAAACCTCTA CACTGACAAA AATGGAGAAG GCGATCAAAC GTCCAATATC 
TCTTTGCAAG ATGACATGGC ATTCAGTCAA GCCTACTCGT GGTCACCCAT GGACAGCTGC 
GATGAGGCTG GCAGGGTGCA GAATGAAAAT ATGACCGAAT TATCTAATCA TGTTGATGAT 
GCTTTGTTGG CAACGCTCAG CTCATCCCCA AATGAAAGCA GCAGGGGATC AAAACGCACG 
ATTGCTGAAG ACATCTACAG TACGCCATCT AAACTACCAA AGCAAACTGC TTCAGAAGAG 
TCTCCAATGG TCAGCTCAAG GCTGAATCTT CTGTCCCTTT CCAGTCCTGA CATCAAATCC 
AGCCGCCAGC TCTTCCAAGA CGATACACAC TCTCACAGGG GTCAGCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELB ( XM_001336741.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001336777.3 Danio rerio helicase (DNA) B (helb), transcript variant X1, mRNA.

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CloneID	ODa09292
Clone ID Related Accession (Same CDS sequence)	XM_001336741.6 , XM_001336741.5
Accession Version	XM_001336741.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	DNA helicase B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_001336741.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTTC AAAGTCCCAT TGCGGGATTA AAAACCATAA CTGGTTACAT TTTACCAGAA 
GAACAAGCTG TCCAGCCAAA CAAAGACGAT GGTTTAGAAA GTGAAGATGA AGAGGAGGAA 
GGACAGCCTG AATTTCTGGA CATGAAGGAG ATGAACTCGG TGTCATCTGG GGGAAACATG 
TTCAAATCTT TAGTGCCAAC ATCAAAAGAA GTGTCAATTC TGGTTGGTAA AAAGACATAC 
AAGGTTGAGG GCCGATTCCC CCTCTGTGAC CCCTGGTGGG AAGTAACCTG TACAATTCGC 
CTAGGCCGAC TCAAGGCATT TGTTAAAGGC TACCCTTTCT ATCGACTCCG CACTCATCTG 
AAATCAGAGG GTCGGTCACT CTTGTCTCTT TTTCTCACCG CATGTAAGGC ACAAGCTGAT 
CATGTTAAAA TGTTTATGGA CTGGTTGCCC ACTGACAGAC GTGTGGAGCT TGTTAATGTG 
CTGGACGTCC TGCAGGAGTT TGAAGATTCC TCACTTGAGC ATGAAGCTGT GGCTCAACAG 
CTCAAGTCAA GTGTCAATTG TTCAGTTGTT TGGACGCACG TAAAAGCTGC CAGCTTGTAC 
CCTGGAATCA TGAAATATCT GCCAACTTTG CTGCCAGGCC AGTATCTGGA CATTATAAAA 
AGAGCAAGAA TAGAAGATAT TCCAGAAACT TCAGCAATGG ATGAAGACCC TCAAAACGAG 
CAGAGAAAAA CAAATGTATT GTCCAAACTT GAGGAGCTAA TAAATACTAA TAATGTGTGG 
AAGCTGGGCT TCAACTATAT CATGTTTAAG GAATTGCATC TGATCAGGTG TGAAGCAAAA 
CTTGAAGCCT TCAAGCTGTG CGATTTGTTT TCAGCTATCC CGCTTCTTCA GCGTAACGCG 
TTGATTTTAT ATGCAGAACT GAAGAAACAC TGCAGAGTCA CCGGAAGCAC TTACATAACT 
CAGGAGATGC TTGTGGACAA AATATTTTCT GAAACCGGGG CGGATGGTGC CTGGGCAGCT 
CTGCACTTCC TTGTGACACA GGGGGTGCTG ATGAAGGAGA GCACCAAGGT TGCACTTCGA 
AACCTGCTGA AGTATGAGAA AGGAATTGCA GAATGTTTGA CAGGCCTGGT TCAAGGAGAC 
CCTTGGAAAA TCCACCTGGA TGTCAAGGAG GTTCTCCGTG AAGCTCAGAT AAAAAGATTA 
AGAGCAAAGG CTTGTGAAAA ACACAATGCA ACTATGAAAT CAAAGGATGA TGCAAACACA 
TCTGCCTCCG CAGCAGAAAA TTCCACCGCT GTGTTTGAGC AGTTAGGCAT TGATTTAGAC 
CAGCCTGGTA CTTCAAGCAT GGTCCACAAT GGAGAAACAC CATCATGTGA TGATTCTGAA 
AATACAACGC TCACTATTAA AGAGGAAAAT CTCACATCAG ACTCTGACTC AGATTTGGAC 
ATTGATCCCA GTACGATAGA GTTGGATTGT GATCAGGTGC GGGCAGCCGA AATGATGTGT 
GCAAATCCGG TTACAGTGAT CAGTGGGAAA GGGGGTTGTG GGAAAACCAC AGTGGTCAGC 
CTGGTGTTTA AGGCGGCCAT GGAGCAGCAG ACAAGCGACT TGGAGGAGGT GCAGAAGGCC 
TGCGAGGACT TTCAGAATGA CTCTCATGGC TCTAGTAATG GGCTGGCGTT GGATGTGCAC 
GAGGAGAAAA ACCATAGCGA GAAGAGCATC AGTAATGAAA AATCTGTGGA AGTTCTCCTC 
ACTGCTCCTA CAGGCAGAGC TGCTTCACTT CTCACTAAAA GAACTGGGTT CACTGCTTAC 
ACCATGCACC AGGTGTTATG GAGTTTCATG ACTGTAGATA AGGATCGAAG TGGGAATCCA 
CAGGACTGGA AGTTCTCAAA GGTTCGGGTG CTGGTGGTTG ACGAGGGAAG CTTGGTTTCT 
GTTCAGATCC TTCACTCCAT TCTTAGCATG CTTACCAAAC ACGCACAACT CCAGAAGTTC 
ATCATTTTGG GTGATGTGCG GCAGTTGCCC AGCATTGAGC CTGGAAACAC ACTATATGAT 
CTGTTTGAAG GTCTCAGAAG AGTCCGCTGG GCTATTGAGA TGAGGACCAA TCATCGAGCC 
GAGTCTGAGC TCATCGTCAG AAATGCTGGA CTCATCTCTG AGATGGGTAA AAAAAAGTAT 
TACAAGCCTC TGGACTTTGA TGCCACCATA GACATGACAA ACCCCTCTGC AGTGCCATCC 
GACAAAAGAT TCATTTTTAT CAAAATCAGT TCGGACAATT TTTCTTTTGA CCTCCAGGAA 
GCCATCTTGT TCCTACTTAA GGAAGCTCCT GGGCTGAAAG ATCATATATT GTCACAGTTT 
GTGGCTTTTC GGAGGAAGGA TTGTGAGTTA ATCAATGAGC TTTGCTGCAG ACATTATTCA 
AACCATACCA CAAAAACTGC CAAGAAAACA TTCAACTTTC AGGTAGGTGA TAAAGTCTGC 
TGCACTAAGA ATGGCTACGT CACAGACTAT GATAAGAAGG AGACGTCTTT GGCGGACATC 
AGAGCTTCAC ATAATCGTGC TGAAAGTCAA CAAGATGATC AGAAGAAAGA AAAGAAAGAG 
AAGAAAGAGC GATTGTGCAA TGGCGAAATT TTCTTCATTA AAGATGATGT GACTAAATCT 
GTCGAGAATG AAAAAAATAA AATGGTGCGT CAACTGACAC TCGATGATAA AAGCGACCGC 
CAGGTGACTT GTAACTACAA AGAGCTACAG AGAGAGTGTA AACTGCGCCA TTCTTGGGCA 
AGAACCATTC ACACCTTTCA GGGCTCAGAA GCTGAAACGA TTGTATACAT TCTTGGAGAC 
AGTCCTGCCC AAAACTGGCA GCATGTTTAC ACCGCGGTGA CTCGTGGTCA GAAGCGTGTG 
TATGTGGTGG GTACAGAGTA TGATCTAGAA GGTGCCATCA GGAAGAGGAT CACCCCTCGT 
AACACACGAC TCTGCGGCTT TGTCAACACT AAAGTTGGCC AACAAGACCC TTTGACTACA 
TCTGCCTCCA CCCAGAGGCA AGCAGAGACT ACCTTGAACC ACAGTTTTGG GCATTCACAA 
TCAACCCCAG TGGCCTCGCA AACTCCAATC CGCCCTCAGA TGTTGTCCAG TACACGACCT 
TCATGTGCAA GAAACCTCTA CACTGACAAA AATGGAGAAG GCGATCAAAC GTCCAATATC 
TCTTTGCAAG ATGACATGGC ATTCAGTCAA GCCTACTCGT GGTCACCCAT GGACAGCTGC 
GATGAGGCTG GCAGGGTGCA GAATGAAAAT ATGACCGAAT TATCTAATCA TGTTGATGAT 
GCTTTGTTGG CAACGCTCAG CTCATCCCCA AATGAAAGCA GCAGGGGATC AAAACGCACG 
ATTGCTGAAG ACATCTACAG TACGCCATCT AAACTACCAA AGCAAACTGC TTCAGAAGAG 
TCTCCAATGG TCAGCTCAAG GCTGAATCTT CTGTCCCTTT CCAGTCCTGA CATCAAATCC 
AGCCGCCAGC TCTTCCAAGA CGATACACAC TCTCACAGGG GTCAGCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001336777.3
CDS	1015..4545
Translation	MALQSPIAGLKTITGYILPEEQAVQPNKDDGLESEDEEEEGQPEFLDMKEMNSVSSGGNMFKSLVPTSKEVSILVGKKTYKVEGRFPLCDPWWEVTCTIRLGRLKAFVKGYPFYRLRTHLKSEGRSLLSLFLTACKAQADHVKMFMDWLPTDRRVELVNVLDVLQEFEDSSLEHEAVAQQLKSSVNCSVVWTHVKAASLYPGIMKYLPTLLPGQYLDIIKRARIEDIPETSAMDEDPQNEQRKTNVLSKLEELINTNNVWKLGFNYIMFKELHLIRCEAKLEAFKLCDLFSAIPLLQRNALILYAELKKHCRVTGSTYITQEMLVDKIFSETGADGAWAALHFLVTQGVLMKESTKVALRNLLKYEKGIAECLTGLVQGDPWKIHLDVKEVLREAQIKRLRAKACEKHNATMKSKDDANTSASAAENSTAVFEQLGIDLDQPGTSSMVHNGETPSCDDSENTTLTIKEENLTSDSDSDLDIDPSTIELDCDQVRAAEMMCANPVTVISGKGGCGKTTVVSLVFKAAMEQQTSDLEEVQKACEDFQNDSHGSSNGLALDVHEEKNHSEKSISNEKSVEVLLTAPTGRAASLLTKRTGFTAYTMHQVLWSFMTVDKDRSGNPQDWKFSKVRVLVVDEGSLVSVQILHSILSMLTKHAQLQKFIILGDVRQLPSIEPGNTLYDLFEGLRRVRWAIEMRTNHRAESELIVRNAGLISEMGKKKYYKPLDFDATIDMTNPSAVPSDKRFIFIKISSDNFSFDLQEAILFLLKEAPGLKDHILSQFVAFRRKDCELINELCCRHYSNHTTKTAKKTFNFQVGDKVCCTKNGYVTDYDKKETSLADIRASHNRAESQQDDQKKEKKEKKERLCNGEIFFIKDDVTKSVENEKNKMVRQLTLDDKSDRQVTCNYKELQRECKLRHSWARTIHTFQGSEAETIVYILGDSPAQNWQHVYTAVTRGQKRVYVVGTEYDLEGAIRKRITPRNTRLCGFVNTKVGQQDPLTTSASTQRQAETTLNHSFGHSQSTPVASQTPIRPQMLSSTRPSCARNLYTDKNGEGDQTSNISLQDDMAFSQAYSWSPMDSCDEAGRVQNENMTELSNHVDDALLATLSSSPNESSRGSKRTIAEDIYSTPSKLPKQTASEESPMVSSRLNLLSLSSPDIKSSRQLFQDDTHSHRGQP

Target ORF information:

RefSeq Version	XM_001336741.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio helicase (DNA) B (helb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001336741.5

ORF Insert Sequence:

ATGGCGCTTC AAAGTCCCAT TGCGGGATTA AAAACCATAA CTGGTTACAT TTTACCAGAA 
GAACAAGCTG TCCAGCCAAA CAAAGACGAT GGTTTAGAAA GTGAAGATGA AGAGGAGGAA 
GGACAGCCTG AATTTCTGGA CATGAAGGAG ATGAACTCGG TGTCATCTGG GGGAAACATG 
TTCAAATCTT TAGTGCCAAC ATCAAAAGAA GTGTCAATTC TGGTTGGTAA AAAGACATAC 
AAGGTTGAGG GCCGATTCCC CCTCTGTGAC CCCTGGTGGG AAGTAACCTG TACAATTCGC 
CTAGGCCGAC TCAAGGCATT TGTTAAAGGC TACCCTTTCT ATCGACTCCG CACTCATCTG 
AAATCAGAGG GTCGGTCACT CTTGTCTCTT TTTCTCACCG CATGTAAGGC ACAAGCTGAT 
CATGTTAAAA TGTTTATGGA CTGGTTGCCC ACTGACAGAC GTGTGGAGCT TGTTAATGTG 
CTGGACGTCC TGCAGGAGTT TGAAGATTCC TCACTTGAGC ATGAAGCTGT GGCTCAACAG 
CTCAAGTCAA GTGTCAATTG TTCAGTTGTT TGGACGCACG TAAAAGCTGC CAGCTTGTAC 
CCTGGAATCA TGAAATATCT GCCAACTTTG CTGCCAGGCC AGTATCTGGA CATTATAAAA 
AGAGCAAGAA TAGAAGATAT TCCAGAAACT TCAGCAATGG ATGAAGACCC TCAAAACGAG 
CAGAGAAAAA CAAATGTATT GTCCAAACTT GAGGAGCTAA TAAATACTAA TAATGTGTGG 
AAGCTGGGCT TCAACTATAT CATGTTTAAG GAATTGCATC TGATCAGGTG TGAAGCAAAA 
CTTGAAGCCT TCAAGCTGTG CGATTTGTTT TCAGCTATCC CGCTTCTTCA GCGTAACGCG 
TTGATTTTAT ATGCAGAACT GAAGAAACAC TGCAGAGTCA CCGGAAGCAC TTACATAACT 
CAGGAGATGC TTGTGGACAA AATATTTTCT GAAACCGGGG CGGATGGTGC CTGGGCAGCT 
CTGCACTTCC TTGTGACACA GGGGGTGCTG ATGAAGGAGA GCACCAAGGT TGCACTTCGA 
AACCTGCTGA AGTATGAGAA AGGAATTGCA GAATGTTTGA CAGGCCTGGT TCAAGGAGAC 
CCTTGGAAAA TCCACCTGGA TGTCAAGGAG GTTCTCCGTG AAGCTCAGAT AAAAAGATTA 
AGAGCAAAGG CTTGTGAAAA ACACAATGCA ACTATGAAAT CAAAGGATGA TGCAAACACA 
TCTGCCTCCG CAGCAGAAAA TTCCACCGCT GTGTTTGAGC AGTTAGGCAT TGATTTAGAC 
CAGCCTGGTA CTTCAAGCAT GGTCCACAAT GGAGAAACAC CATCATGTGA TGATTCTGAA 
AATACAACGC TCACTATTAA AGAGGAAAAT CTCACATCAG ACTCTGACTC AGATTTGGAC 
ATTGATCCCA GTACGATAGA GTTGGATTGT GATCAGGTGC GGGCAGCCGA AATGATGTGT 
GCAAATCCGG TTACAGTGAT CAGTGGGAAA GGGGGTTGTG GGAAAACCAC AGTGGTCAGC 
CTGGTGTTTA AGGCGGCCAT GGAGCAGCAG ACAAGCGACT TGGAGGAGGT GCAGAAGGCC 
TGCGAGGACT TTCAGAATGA CTCTCATGGC TCTAGTAATG GGCTGGCGTT GGATGTGCAC 
GAGGAGAAAA ACCATAGCGA GAAGAGCATC AGTAATGAAA AATCTGTGGA AGTTCTCCTC 
ACTGCTCCTA CAGGCAGAGC TGCTTCACTT CTCACTAAAA GAACTGGGTT CACTGCTTAC 
ACCATGCACC AGGTGTTATG GAGTTTCATG ACTGTAGATA AGGATCGAAG TGGGAATCCA 
CAGGACTGGA AGTTCTCAAA GGTTCGGGTG CTGGTGGTTG ACGAGGGAAG CTTGGTTTCT 
GTTCAGATCC TTCACTCCAT TCTTAGCATG CTTACCAAAC ACGCACAACT CCAGAAGTTC 
ATCATTTTGG GTGATGTGCG GCAGTTGCCC AGCATTGAGC CTGGAAACAC ACTATATGAT 
CTGTTTGAAG GTCTCAGAAG AGTCCGCTGG GCTATTGAGA TGAGGACCAA TCATCGAGCC 
GAGTCTGAGC TCATCGTCAG AAATGCTGGA CTCATCTCTG AGATGGGTAA AAAAAAGTAT 
TACAAGCCTC TGGACTTTGA TGCCACCATA GACATGACAA ACCCCTCTGC AGTGCCATCC 
GACAAAAGAT TCATTTTTAT CAAAATCAGT TCGGACAATT TTTCTTTTGA CCTCCAGGAA 
GCCATCTTGT TCCTACTTAA GGAAGCTCCT GGGCTGAAAG ATCATATATT GTCACAGTTT 
GTGGCTTTTC GGAGGAAGGA TTGTGAGTTA ATCAATGAGC TTTGCTGCAG ACATTATTCA 
AACCATACCA CAAAAACTGC CAAGAAAACA TTCAACTTTC AGGTAGGTGA TAAAGTCTGC 
TGCACTAAGA ATGGCTACGT CACAGACTAT GATAAGAAGG AGACGTCTTT GGCGGACATC 
AGAGCTTCAC ATAATCGTGC TGAAAGTCAA CAAGATGATC AGAAGAAAGA AAAGAAAGAG 
AAGAAAGAGC GATTGTGCAA TGGCGAAATT TTCTTCATTA AAGATGATGT GACTAAATCT 
GTCGAGAATG AAAAAAATAA AATGGTGCGT CAACTGACAC TCGATGATAA AAGCGACCGC 
CAGGTGACTT GTAACTACAA AGAGCTACAG AGAGAGTGTA AACTGCGCCA TTCTTGGGCA 
AGAACCATTC ACACCTTTCA GGGCTCAGAA GCTGAAACGA TTGTATACAT TCTTGGAGAC 
AGTCCTGCCC AAAACTGGCA GCATGTTTAC ACCGCGGTGA CTCGTGGTCA GAAGCGTGTG 
TATGTGGTGG GTACAGAGTA TGATCTAGAA GGTGCCATCA GGAAGAGGAT CACCCCTCGT 
AACACACGAC TCTGCGGCTT TGTCAACACT AAAGTTGGCC AACAAGACCC TTTGACTACA 
TCTGCCTCCA CCCAGAGGCA AGCAGAGACT ACCTTGAACC ACAGTTTTGG GCATTCACAA 
TCAACCCCAG TGGCCTCGCA AACTCCAATC CGCCCTCAGA TGTTGTCCAG TACACGACCT 
TCATGTGCAA GAAACCTCTA CACTGACAAA AATGGAGAAG GCGATCAAAC GTCCAATATC 
TCTTTGCAAG ATGACATGGC ATTCAGTCAA GCCTACTCGT GGTCACCCAT GGACAGCTGC 
GATGAGGCTG GCAGGGTGCA GAATGAAAAT ATGACCGAAT TATCTAATCA TGTTGATGAT 
GCTTTGTTGG CAACGCTCAG CTCATCCCCA AATGAAAGCA GCAGGGGATC AAAACGCACG 
ATTGCTGAAG ACATCTACAG TACGCCATCT AAACTACCAA AGCAAACTGC TTCAGAAGAG 
TCTCCAATGG TCAGCTCAAG GCTGAATCTT CTGTCCCTTT CCAGTCCTGA CATCAAATCC 
AGCCGCCAGC TCTTCCAAGA CGATACACAC TCTCACAGGG GTCAGCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HELB cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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