Amd1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Amd1
Entrez Gene ID	11702
Full Name	S-adenosylmethionine decarboxylase 1
Synonyms	AdoMetDC,Amd-1,SAMDC,SAMDC 1,adoMetDC1
General protein information	Preferred Names S-adenosylmethionine decarboxylase 1 Names S-adenosylmethionine decarboxylase proenzyme 1 adoMetDC 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 B1\|10 21.85 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009665.5	NP_033795.1	S-adenosylmethionine decarboxylase proenzyme 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Amd1	NP_112273.2
Danio rerio (zebrafish)	amd1	NP_001155073.1
Drosophila melanogaster (fruit fly)	SamDC	NP_477223.2
Oryza sativa (rice)	Os04g0498600	NP_001053213.1
Mus musculus (house mouse)	Amd2	NP_031470.3
Homo sapiens (human)	AMD1	NP_001625.2
Gallus gallus (chicken)	AMD1	NP_001012587.1
Arabidopsis thaliana (thale cress)	AT5G15950	NP_001031888.1
Arabidopsis thaliana (thale cress)	SAMDC	NP_186896.1
Schizosaccharomyces pombe (fission yeast)	spe2	NP_596179.1
Neurospora crassa	NCU01083	XP_961695.1
Pan troglodytes (chimpanzee)	AMD1	XP_001155870.1
Saccharomyces cerevisiae (baker's yeast)	SPE2	NP_014590.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009619	XP_318648.4
Canis lupus familiaris (dog)	AMD1	XP_539081.2
Bos taurus (cattle)	AMD1	NP_776415.1
Mus musculus (house mouse)	Amd1	NP_033795.1
Arabidopsis thaliana (thale cress)	AT3G25570	NP_189184.1
Xenopus tropicalis (tropical clawed frog)	amd1	NP_001017143.1
Oryza sativa (rice)	Os09g0424300	NP_001063217.1
Kluyveromyces lactis	KLLA0C14410g	XP_452845.1
Eremothecium gossypii	AGOS_ABL061C	NP_982886.1
Magnaporthe oryzae (rice blast fungus)	MGG_14297	XP_003710731.1
Caenorhabditis elegans (roundworm)	smd-1	NP_493448.1
Oryza sativa (rice)	Os02g0611200	NP_001047404.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of polyamine synthesis causes entry of the mouse blastocyst into embryonic diapause.
Fenelon JC, Murphy BD
Biology of reproduction97(1)119-132(2017 Jul)

AMD1 is essential for ESC self-renewal and is translationally down-regulated on differentiation to neural precursor cells.
Zhang D, Zhao T, Ang HS, Chong P, Saiki R, Igarashi K, Yang H, Vardy LA
Genes & development26(5)461-73(2012 Mar)

Transcriptomic analysis of polyamine-related genes and polyamine levels in placenta, yolk sac and fetus during the second half of mouse pregnancy.
Lopez-Garcia C, Lopez-Contreras AJ, Cremades A, Castells MT, Peñafiel R
Placenta30(3)241-9(2009 Mar)

Molecular and morphological changes in placenta and embryo development associated with the inhibition of polyamine synthesis during midpregnancy in mice.
López-García C, López-Contreras AJ, Cremades A, Castells MT, Marín F, Schreiber F, Peñafiel R
Endocrinology149(10)5012-23(2008 Oct)

Regulated translation termination at the upstream open reading frame in s-adenosylmethionine decarboxylase mRNA.
Raney A, Law GL, Mize GJ, Morris DR
The Journal of biological chemistry277(8)5988-94(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Amd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Amd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18403	NM_009665.5 Latest version!	Mus musculus S-adenosylmethionine decarboxylase 1 (Amd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Amd1 ( NM_009665.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033795.1 Mus musculus S-adenosylmethionine decarboxylase 1 (Amd1), mRNA.

Price & Availability↑

CloneID	OMu18403
Clone ID Related Accession (Same CDS sequence)	NM_009665.5
Accession Version	NM_009665.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Mus musculus(house mouse)
Product	S-adenosylmethionine decarboxylase proenzyme 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC142273.3 and AK146840.1. On Mar 19, 2011 this sequence version replaced NM_009665.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011110.1, AK146840.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 10829027 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCTG CACATTTTTT CGAAGGGACG GAGAAACTGC TGGAGGTCTG GTTTTCCAGA 
CAGCAGTCCG ACGCAAGCCA GGGATCTGGG GATCTTCGTA CCATCCCAAG ATCTGAATGG 
GATGTCCTTT TGAAGGATGT GCAGTGCTCA ATCATAAGTG TGACAAAGAC TGACAAGCAG 
GAAGCTTATG TACTCAGTGA GAGTAGCATG TTTGTCTCCA AGAGACGTTT CATTTTGAAG 
ACATGTGGTA CCACCCTCTT ACTGAAAGCA CTGGTTCCCC TGTTGAAGCT TGCTAGGGAT 
TACAGTGGGT TTGACTCAAT TCAAAGCTTC TTTTATTCTC GTAAGAATTT CATGAAGCCT 
TCTCACCAAG GGTACCCACA CCGGAATTTC CAGGAAGAAA TCGAGTTTCT TAATGCAATT 
TTCCCAAATG GAGCAGCATA TTGTATGGGA CGCATGAATT CTGACTGCTG GTACTTGTAT 
ACTTTGGATT TCCCAGAGAG CCGAGTAATC AGTCAGCCAG ATCAAACACT GGAAATTCTG 
ATGAGTGAGC TTGACCCAGC AGTTATGGAC CAGTTCTACA TGAAAGATGG TGTTACTGCA 
AAGGATGTCA CTCGTGAGAG TGGAATTCGT GACCTGATAC CAGGTTCTGT CATTGATGCC 
ACACTGTTCA ATCCTTGTGG CTACTCGATG AATGGAATGA AATCGGATGG AACATATTGG 
ACTATTCACA TCACTCCAGA ACCAGAATTT TCTTATGTTA GCTTTGAAAC AAACCTAAGT 
CAGACCTCCT ATGACGACCT GATCAGGAAA GTTGTGGAAG TCTTCAAGCC AGGAAAATTT 
GTGACCACCT TGTTTGTTAA TCAGAGTTCT AAATGTCGCA CAGTGCTTTC TTCGCCCCAG 
AAGATTGACG GCTTTAAACG TCTTGATTGC CAGAGTGCTA TGTTCAACGA TTACAATTTT 
GTTTTTACCA GTTTTGCTAA GAAACAGCAA CAACAGCAGA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033795.1
CDS	331..1335
Translation	MEAAHFFEGTEKLLEVWFSRQQSDASQGSGDLRTIPRSEWDVLLKDVQCSIISVTKTDKQEAYVLSESSMFVSKRRFILKTCGTTLLLKALVPLLKLARDYSGFDSIQSFFYSRKNFMKPSHQGYPHRNFQEEIEFLNAIFPNGAAYCMGRMNSDCWYLYTLDFPESRVISQPDQTLEILMSELDPAVMDQFYMKDGVTAKDVTRESGIRDLIPGSVIDATLFNPCGYSMNGMKSDGTYWTIHITPEPEFSYVSFETNLSQTSYDDLIRKVVEVFKPGKFVTTLFVNQSSKCRTVLSSPQKIDGFKRLDCQSAMFNDYNFVFTSFAKKQQQQQS

Target ORF information:

RefSeq Version	NM_009665.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus S-adenosylmethionine decarboxylase 1 (Amd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009665.5

ORF Insert Sequence:

ATGGAAGCTG CACATTTTTT CGAAGGGACG GAGAAACTGC TGGAGGTCTG GTTTTCCAGA 
CAGCAGTCCG ACGCAAGCCA GGGATCTGGG GATCTTCGTA CCATCCCAAG ATCTGAATGG 
GATGTCCTTT TGAAGGATGT GCAGTGCTCA ATCATAAGTG TGACAAAGAC TGACAAGCAG 
GAAGCTTATG TACTCAGTGA GAGTAGCATG TTTGTCTCCA AGAGACGTTT CATTTTGAAG 
ACATGTGGTA CCACCCTCTT ACTGAAAGCA CTGGTTCCCC TGTTGAAGCT TGCTAGGGAT 
TACAGTGGGT TTGACTCAAT TCAAAGCTTC TTTTATTCTC GTAAGAATTT CATGAAGCCT 
TCTCACCAAG GGTACCCACA CCGGAATTTC CAGGAAGAAA TCGAGTTTCT TAATGCAATT 
TTCCCAAATG GAGCAGCATA TTGTATGGGA CGCATGAATT CTGACTGCTG GTACTTGTAT 
ACTTTGGATT TCCCAGAGAG CCGAGTAATC AGTCAGCCAG ATCAAACACT GGAAATTCTG 
ATGAGTGAGC TTGACCCAGC AGTTATGGAC CAGTTCTACA TGAAAGATGG TGTTACTGCA 
AAGGATGTCA CTCGTGAGAG TGGAATTCGT GACCTGATAC CAGGTTCTGT CATTGATGCC 
ACACTGTTCA ATCCTTGTGG CTACTCGATG AATGGAATGA AATCGGATGG AACATATTGG 
ACTATTCACA TCACTCCAGA ACCAGAATTT TCTTATGTTA GCTTTGAAAC AAACCTAAGT 
CAGACCTCCT ATGACGACCT GATCAGGAAA GTTGTGGAAG TCTTCAAGCC AGGAAAATTT 
GTGACCACCT TGTTTGTTAA TCAGAGTTCT AAATGTCGCA CAGTGCTTTC TTCGCCCCAG 
AAGATTGACG GCTTTAAACG TCTTGATTGC CAGAGTGCTA TGTTCAACGA TTACAATTTT 
GTTTTTACCA GTTTTGCTAA GAAACAGCAA CAACAGCAGA GTTGA