AMD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AMD1
Entrez Gene ID	262
Full Name	adenosylmethionine decarboxylase 1
Synonyms	ADOMETDC,AMD,SAMDC
General protein information	Preferred Names adenosylmethionine decarboxylase 1 Names S-adenosylmethionine decarboxylase proenzyme S-adenosylmethionine decarboxylase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q21
Summary	This gene encodes an important intermediate enzyme in polyamine biosynthesis. The polyamines spermine, spermidine, and putrescine are low-molecular-weight aliphatic amines essential for cellular proliferation and tumor promotion. Multiple alternatively spliced transcript variants have been identified. Pseudogenes of this gene are found on chromosomes 5, 6, 10, X and Y. [provided by RefSeq, Dec 2013].
Disorder MIM:	180980

mRNA and Protein(s)

mRNA	Protein	Name
NM_001287215.1	NP_001274144.1	S-adenosylmethionine decarboxylase proenzyme isoform 4
NM_001634.5	NP_001625.2	S-adenosylmethionine decarboxylase proenzyme isoform 1
NM_001287214.1	NP_001274143.1	S-adenosylmethionine decarboxylase proenzyme isoform 3
NM_001287216.1	NP_001274145.1	S-adenosylmethionine decarboxylase proenzyme isoform 5

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Amd1	NP_112273.2
Danio rerio (zebrafish)	amd1	NP_001155073.1
Drosophila melanogaster (fruit fly)	SamDC	NP_477223.2
Oryza sativa (rice)	Os04g0498600	NP_001053213.1
Mus musculus (house mouse)	Amd2	NP_031470.3
Homo sapiens (human)	AMD1	NP_001625.2
Gallus gallus (chicken)	AMD1	NP_001012587.1
Arabidopsis thaliana (thale cress)	AT5G15950	NP_001031888.1
Arabidopsis thaliana (thale cress)	SAMDC	NP_186896.1
Schizosaccharomyces pombe (fission yeast)	spe2	NP_596179.1
Neurospora crassa	NCU01083	XP_961695.1
Pan troglodytes (chimpanzee)	AMD1	XP_001155870.1
Saccharomyces cerevisiae (baker's yeast)	SPE2	NP_014590.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009619	XP_318648.4
Canis lupus familiaris (dog)	AMD1	XP_539081.2
Bos taurus (cattle)	AMD1	NP_776415.1
Mus musculus (house mouse)	Amd1	NP_033795.1
Arabidopsis thaliana (thale cress)	AT3G25570	NP_189184.1
Xenopus tropicalis (tropical clawed frog)	amd1	NP_001017143.1
Oryza sativa (rice)	Os09g0424300	NP_001063217.1
Kluyveromyces lactis	KLLA0C14410g	XP_452845.1
Eremothecium gossypii	AGOS_ABL061C	NP_982886.1
Magnaporthe oryzae (rice blast fungus)	MGG_14297	XP_003710731.1
Caenorhabditis elegans (roundworm)	smd-1	NP_493448.1
Oryza sativa (rice)	Os02g0611200	NP_001047404.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic variants in RUNX3, AMD1 and MSRA in the methionine metabolic pathway and survival in nonsmall cell lung cancer patients.
Chen K, Liu H, Liu Z, Luo S, Patz EF Jr, Moorman PG, Su L, Shen S, Christiani DC, Wei Q
International journal of cancer145(3)621-631(2019 08)

Polyamine Regulator AMD1 Promotes Cell Migration in Epidermal Wound Healing.
Lim HK, Rahim AB, Leo VI, Das S, Lim TC, Uemura T, Igarashi K, Common J, Vardy LA
The Journal of investigative dermatology138(12)2653-2665(2018 12)

AMD1 mRNA employs ribosome stalling as a mechanism for molecular memory formation.
Yordanova MM, Loughran G, Zhdanov AV, Mariotti M, Kiniry SJ, O'Connor PBF, Andreev DE, Tzani I, Saffert P, Michel AM, Gladyshev VN, Papkovsky DB, Atkins JF, Baranov PV
Nature553(7688)356-360(2018 01)

mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer.
Zabala-Letona A, Arruabarrena-Aristorena A, Martín-Martín N, Fernandez-Ruiz S, Sutherland JD, Clasquin M, Tomas-Cortazar J, Jimenez J, Torres I, Quang P, Ximenez-Embun P, Bago R, Ugalde-Olano A, Loizaga-Iriarte A, Lacasa-Viscasillas I, Unda M, Torrano V, Cabrera D, van Liempd SM, Cendon Y, Castro E, Murray S, Revandkar A, Alimonti A, Zhang Y, Barnett A, Lein G, Pirman D, Cortazar AR, Arreal L, Prudkin L, Astobiza I, Valcarcel-Jimenez L, Zuñiga-García P, Fernandez-Dominguez I, Piva M, Caro-Maldonado A, Sánchez-Mosquera P, Castillo-Martín M, Serra V, Beraza N, Gentilella A, Thomas G, Azkargorta M, Elortza F, Farràs R, Olmos D, Efeyan A, Anguita J, Muñoz J, Falcón-Pérez JM, Barrio R, Macarulla T, Mato JM, Martinez-Chantar ML, Cordon-Cardo C, Aransay AM, Marks K, Baselga J, Tabernero J, Nuciforo P, Manning BD, Marjon K, Carracedo A
Nature547(7661)109-113(2017 07)

Regulated translation termination at the upstream open reading frame in s-adenosylmethionine decarboxylase mRNA.
Raney A, Law GL, Mize GJ, Morris DR
The Journal of biological chemistry277(8)5988-94(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AMD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06030	NM_001287215.1 Latest version!	Homo sapiens adenosylmethionine decarboxylase 1 (AMD1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu10046	NM_001287216.1 Latest version!	Homo sapiens adenosylmethionine decarboxylase 1 (AMD1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu10049	NM_001287214.1 Latest version!	Homo sapiens adenosylmethionine decarboxylase 1 (AMD1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu09815	NM_001634.5 Latest version!	Homo sapiens adenosylmethionine decarboxylase 1 (AMD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AMD1 ( NM_001287215.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274144.1 Homo sapiens adenosylmethionine decarboxylase 1 (AMD1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu06030
Clone ID Related Accession (Same CDS sequence)	NM_001287215.1
Accession Version	NM_001287215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576944000000
Organism	Homo sapiens(human)
Product	S-adenosylmethionine decarboxylase proenzyme isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK304351.1, AK130474.1 and AA808049.1. On Dec 10, 2013 this sequence version replaced XM_005266925.1. Transcript Variant: This variant (4) has an alternate 5' exon, which results in translation initiation at a downstream AUG start codon, compared to variant 1. The resulting isoform (4) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304351.1, SRR1803611.75053.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

ATGTTTGTCT CCAAGAGACG TTTCATTTTG AAGACATGTG GTACCACCCT CTTGCTGAAA 
GCACTGGTTC CCCTGTTGAA GCTTGCTAGG GATTACAGTG GGTTTGACTC AATTCAAAGC 
TTCTTTTATT CTCGTAAGAA TTTCATGAAG CCTTCTCACC AAGGGTACCC ACACCGGAAT 
TTCCAGGAAG AAATAGAGTT TCTTAATGCA ATTTTCCCAA ATGGAGCAGC ATATTGTATG 
GGACGTATGA ATTCTGACTG TTGGTACTTA TATACTCTGG ATTTCCCAGA GAGTCGGGTA 
ATCAGTCAGC CAGATCAAAC CTTGGAAATT CTGATGAGTG AGCTTGACCC AGCAGTTATG 
GACCAGTTCT ACATGAAAGA TGGTGTTACT GCAAAGGATG TCACTCGTGA GAGTGGAATT 
CGTGACCTGA TACCAGGTTC TGTCATTGAT GCCACAATGT TCAATCCTTG TGGGTATTCG 
ATGAATGGAA TGAAATCGGA TGGAACTTAT TGGACTATTC ACATCACTCC AGAACCAGAA 
TTTTCTTATG TTAGCTTTGA AACAAACTTA AGTCAGACCT CCTATGATGA CCTGATCAGG 
AAAGTTGTAG AAGTCTTCAA GCCAGGAAAA TTTGTGACCA CCTTGTTTGT TAATCAGAGT 
TCTAAATGTC GCACAGTGCT TGCTTCGCCC CAGAAGATTG AAGGTTTTAA GCGTCTTGAT 
TGCCAGAGTG CTATGTTCAA TGATTACAAT TTTGTTTTTA CCAGTTTTGC TAAGAAGCAG 
CAACAACAGC AGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274144.1
CDS	293..1090
Translation	MFVSKRRFILKTCGTTLLLKALVPLLKLARDYSGFDSIQSFFYSRKNFMKPSHQGYPHRNFQEEIEFLNAIFPNGAAYCMGRMNSDCWYLYTLDFPESRVISQPDQTLEILMSELDPAVMDQFYMKDGVTAKDVTRESGIRDLIPGSVIDATMFNPCGYSMNGMKSDGTYWTIHITPEPEFSYVSFETNLSQTSYDDLIRKVVEVFKPGKFVTTLFVNQSSKCRTVLASPQKIEGFKRLDCQSAMFNDYNFVFTSFAKKQQQQQS

Target ORF information:

RefSeq Version	NM_001287215.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adenosylmethionine decarboxylase 1 (AMD1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287215.1

ORF Insert Sequence:

ATGTTTGTCT CCAAGAGACG TTTCATTTTG AAGACATGTG GTACCACCCT CTTGCTGAAA 
GCACTGGTTC CCCTGTTGAA GCTTGCTAGG GATTACAGTG GGTTTGACTC AATTCAAAGC 
TTCTTTTATT CTCGTAAGAA TTTCATGAAG CCTTCTCACC AAGGGTACCC ACACCGGAAT 
TTCCAGGAAG AAATAGAGTT TCTTAATGCA ATTTTCCCAA ATGGAGCAGC ATATTGTATG 
GGACGTATGA ATTCTGACTG TTGGTACTTA TATACTCTGG ATTTCCCAGA GAGTCGGGTA 
ATCAGTCAGC CAGATCAAAC CTTGGAAATT CTGATGAGTG AGCTTGACCC AGCAGTTATG 
GACCAGTTCT ACATGAAAGA TGGTGTTACT GCAAAGGATG TCACTCGTGA GAGTGGAATT 
CGTGACCTGA TACCAGGTTC TGTCATTGAT GCCACAATGT TCAATCCTTG TGGGTATTCG 
ATGAATGGAA TGAAATCGGA TGGAACTTAT TGGACTATTC ACATCACTCC AGAACCAGAA 
TTTTCTTATG TTAGCTTTGA AACAAACTTA AGTCAGACCT CCTATGATGA CCTGATCAGG 
AAAGTTGTAG AAGTCTTCAA GCCAGGAAAA TTTGTGACCA CCTTGTTTGT TAATCAGAGT 
TCTAAATGTC GCACAGTGCT TGCTTCGCCC CAGAAGATTG AAGGTTTTAA GCGTCTTGAT 
TGCCAGAGTG CTATGTTCAA TGATTACAAT TTTGTTTTTA CCAGTTTTGC TAAGAAGCAG 
CAACAACAGC AGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMD1 ( NM_001287216.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274145.1 Homo sapiens adenosylmethionine decarboxylase 1 (AMD1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu10046
Clone ID Related Accession (Same CDS sequence)	NM_001287216.1
Accession Version	NM_001287216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Homo sapiens(human)
Product	S-adenosylmethionine decarboxylase proenzyme isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302761.1, AK130474.1 and AA808049.1. On Dec 10, 2013 this sequence version replaced XM_005266926.1. Transcript Variant: This variant (5) lacks consecutive four exons in the coding region, but maintains the reading frame, compared to variant 1. The resulting isoform (5) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302761.1, SRR1660809.135598.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 8416975, 10829027, 11741992 ##RefSeq-Attributes-END##

ATGGAAGCTG CACATTTTTT CGAAGGGACC GAGAAGCTGC TGGAGGTTTG GTTCTCCCGG 
CAGCAGCCCG ACGCAAACCA AGGATCTGGG GATCTTCGCA CTATCCCAAG GTACTTATAT 
ACTCTGGATT TCCCAGAGAG TCGGGTAATC AGTCAGCCAG ATCAAACCTT GGAAATTCTG 
ATGAGTGAGC TTGACCCAGC AGTTATGGAC CAGTTCTACA TGAAAGATGG TGTTACTGCA 
AAGGATGTCA CTCGTGAGAG TGGAATTCGT GACCTGATAC CAGGTTCTGT CATTGATGCC 
ACAATGTTCA ATCCTTGTGG GTATTCGATG AATGGAATGA AATCGGATGG AACTTATTGG 
ACTATTCACA TCACTCCAGA ACCAGAATTT TCTTATGTTA GCTTTGAAAC AAACTTAAGT 
CAGACCTCCT ATGATGACCT GATCAGGAAA GTTGTAGAAG TCTTCAAGCC AGGAAAATTT 
GTGACCACCT TGTTTGTTAA TCAGAGTTCT AAATGTCGCA CAGTGCTTGC TTCGCCCCAG 
AAGATTGAAG GTTTTAAGCG TCTTGATTGC CAGAGTGCTA TGTTCAATGA TTACAATTTT 
GTTTTTACCA GTTTTGCTAA GAAGCAGCAA CAACAGCAGA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274145.1
CDS	323..967
Translation	MEAAHFFEGTEKLLEVWFSRQQPDANQGSGDLRTIPRYLYTLDFPESRVISQPDQTLEILMSELDPAVMDQFYMKDGVTAKDVTRESGIRDLIPGSVIDATMFNPCGYSMNGMKSDGTYWTIHITPEPEFSYVSFETNLSQTSYDDLIRKVVEVFKPGKFVTTLFVNQSSKCRTVLASPQKIEGFKRLDCQSAMFNDYNFVFTSFAKKQQQQQS

Target ORF information:

RefSeq Version	NM_001287216.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adenosylmethionine decarboxylase 1 (AMD1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287216.1

ORF Insert Sequence:

ATGGAAGCTG CACATTTTTT CGAAGGGACC GAGAAGCTGC TGGAGGTTTG GTTCTCCCGG 
CAGCAGCCCG ACGCAAACCA AGGATCTGGG GATCTTCGCA CTATCCCAAG GTACTTATAT 
ACTCTGGATT TCCCAGAGAG TCGGGTAATC AGTCAGCCAG ATCAAACCTT GGAAATTCTG 
ATGAGTGAGC TTGACCCAGC AGTTATGGAC CAGTTCTACA TGAAAGATGG TGTTACTGCA 
AAGGATGTCA CTCGTGAGAG TGGAATTCGT GACCTGATAC CAGGTTCTGT CATTGATGCC 
ACAATGTTCA ATCCTTGTGG GTATTCGATG AATGGAATGA AATCGGATGG AACTTATTGG 
ACTATTCACA TCACTCCAGA ACCAGAATTT TCTTATGTTA GCTTTGAAAC AAACTTAAGT 
CAGACCTCCT ATGATGACCT GATCAGGAAA GTTGTAGAAG TCTTCAAGCC AGGAAAATTT 
GTGACCACCT TGTTTGTTAA TCAGAGTTCT AAATGTCGCA CAGTGCTTGC TTCGCCCCAG 
AAGATTGAAG GTTTTAAGCG TCTTGATTGC CAGAGTGCTA TGTTCAATGA TTACAATTTT 
GTTTTTACCA GTTTTGCTAA GAAGCAGCAA CAACAGCAGA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMD1 ( NM_001287214.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274143.1 Homo sapiens adenosylmethionine decarboxylase 1 (AMD1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu10049
Clone ID Related Accession (Same CDS sequence)	NM_001287214.1
Accession Version	NM_001287214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Homo sapiens(human)
Product	S-adenosylmethionine decarboxylase proenzyme isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302761.1, AK300330.1, AK130474.1 and AA808049.1. On Dec 10, 2013 this sequence version replaced XM_005266924.1. Transcript Variant: This variant (3) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300330.1, SRR1803611.109662.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 8416975, 10829027, 11741992 ##RefSeq-Attributes-END##

ATGGAAGCTG CACATTTTTT CGAAGGGACC GAGAAGCTGC TGGAGGTTTG GTTCTCCCGG 
CAGCAGCCCG ACGCAAACCA AGGATCTGGG GATCTTCGCA CTATCCCAAG TGAGAGTAGC 
ATGTTTGTCT CCAAGAGACG TTTCATTTTG AAGACATGTG GTACCACCCT CTTGCTGAAA 
GCACTGGTTC CCCTGTTGAA GCTTGCTAGG GATTACAGTG GGTTTGACTC AATTCAAAGC 
TTCTTTTATT CTCGTAAGAA TTTCATGAAG CCTTCTCACC AAGGGTACCC ACACCGGAAT 
TTCCAGGAAG AAATAGAGTT TCTTAATGCA ATTTTCCCAA ATGGAGCAGC ATATTGTATG 
GGACGTATGA ATTCTGACTG TTGGTACTTA TATACTCTGG ATTTCCCAGA GAGTCGGGTA 
ATCAGTCAGC CAGATCAAAC CTTGGAAATT CTGATGAGTG AGCTTGACCC AGCAGTTATG 
GACCAGTTCT ACATGAAAGA TGGTGTTACT GCAAAGGATG TCACTCGTGA GAGTGGAATT 
CGTGACCTGA TACCAGGTTC TGTCATTGAT GCCACAATGT TCAATCCTTG TGGGTATTCG 
ATGAATGGAA TGAAATCGGA TGGAACTTAT TGGACTATTC ACATCACTCC AGAACCAGAA 
TTTTCTTATG TTAGCTTTGA AACAAACTTA AGTCAGACCT CCTATGATGA CCTGATCAGG 
AAAGTTGTAG AAGTCTTCAA GCCAGGAAAA TTTGTGACCA CCTTGTTTGT TAATCAGAGT 
TCTAAATGTC GCACAGTGCT TGCTTCGCCC CAGAAGATTG AAGGTTTTAA GCGTCTTGAT 
TGCCAGAGTG CTATGTTCAA TGATTACAAT TTTGTTTTTA CCAGTTTTGC TAAGAAGCAG 
CAACAACAGC AGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274143.1
CDS	323..1240
Translation	MEAAHFFEGTEKLLEVWFSRQQPDANQGSGDLRTIPSESSMFVSKRRFILKTCGTTLLLKALVPLLKLARDYSGFDSIQSFFYSRKNFMKPSHQGYPHRNFQEEIEFLNAIFPNGAAYCMGRMNSDCWYLYTLDFPESRVISQPDQTLEILMSELDPAVMDQFYMKDGVTAKDVTRESGIRDLIPGSVIDATMFNPCGYSMNGMKSDGTYWTIHITPEPEFSYVSFETNLSQTSYDDLIRKVVEVFKPGKFVTTLFVNQSSKCRTVLASPQKIEGFKRLDCQSAMFNDYNFVFTSFAKKQQQQQS

Target ORF information:

RefSeq Version	NM_001287214.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adenosylmethionine decarboxylase 1 (AMD1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287214.1

ORF Insert Sequence:

ATGGAAGCTG CACATTTTTT CGAAGGGACC GAGAAGCTGC TGGAGGTTTG GTTCTCCCGG 
CAGCAGCCCG ACGCAAACCA AGGATCTGGG GATCTTCGCA CTATCCCAAG TGAGAGTAGC 
ATGTTTGTCT CCAAGAGACG TTTCATTTTG AAGACATGTG GTACCACCCT CTTGCTGAAA 
GCACTGGTTC CCCTGTTGAA GCTTGCTAGG GATTACAGTG GGTTTGACTC AATTCAAAGC 
TTCTTTTATT CTCGTAAGAA TTTCATGAAG CCTTCTCACC AAGGGTACCC ACACCGGAAT 
TTCCAGGAAG AAATAGAGTT TCTTAATGCA ATTTTCCCAA ATGGAGCAGC ATATTGTATG 
GGACGTATGA ATTCTGACTG TTGGTACTTA TATACTCTGG ATTTCCCAGA GAGTCGGGTA 
ATCAGTCAGC CAGATCAAAC CTTGGAAATT CTGATGAGTG AGCTTGACCC AGCAGTTATG 
GACCAGTTCT ACATGAAAGA TGGTGTTACT GCAAAGGATG TCACTCGTGA GAGTGGAATT 
CGTGACCTGA TACCAGGTTC TGTCATTGAT GCCACAATGT TCAATCCTTG TGGGTATTCG 
ATGAATGGAA TGAAATCGGA TGGAACTTAT TGGACTATTC ACATCACTCC AGAACCAGAA 
TTTTCTTATG TTAGCTTTGA AACAAACTTA AGTCAGACCT CCTATGATGA CCTGATCAGG 
AAAGTTGTAG AAGTCTTCAA GCCAGGAAAA TTTGTGACCA CCTTGTTTGT TAATCAGAGT 
TCTAAATGTC GCACAGTGCT TGCTTCGCCC CAGAAGATTG AAGGTTTTAA GCGTCTTGAT 
TGCCAGAGTG CTATGTTCAA TGATTACAAT TTTGTTTTTA CCAGTTTTGC TAAGAAGCAG 
CAACAACAGC AGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMD1 ( NM_001634.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001625.2 Homo sapiens adenosylmethionine decarboxylase 1 (AMD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09815
Clone ID Related Accession (Same CDS sequence)	NM_001634.5
Accession Version	NM_001634.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Homo sapiens(human)
Product	S-adenosylmethionine decarboxylase proenzyme isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302761.1, BX640599.1, AK300330.1, AK130474.1 and AA808049.1. On Dec 10, 2013 this sequence version replaced NM_001634.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.187809.1, BX640599.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein regulatory uORF :: PMID: 8416975, 10829027, 11741992 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAAGCTG CACATTTTTT CGAAGGGACC GAGAAGCTGC TGGAGGTTTG GTTCTCCCGG 
CAGCAGCCCG ACGCAAACCA AGGATCTGGG GATCTTCGCA CTATCCCAAG ATCTGAGTGG 
GACATACTTT TGAAGGATGT GCAATGTTCA ATCATAAGTG TGACAAAAAC TGACAAGCAG 
GAAGCTTATG TACTCAGTGA GAGTAGCATG TTTGTCTCCA AGAGACGTTT CATTTTGAAG 
ACATGTGGTA CCACCCTCTT GCTGAAAGCA CTGGTTCCCC TGTTGAAGCT TGCTAGGGAT 
TACAGTGGGT TTGACTCAAT TCAAAGCTTC TTTTATTCTC GTAAGAATTT CATGAAGCCT 
TCTCACCAAG GGTACCCACA CCGGAATTTC CAGGAAGAAA TAGAGTTTCT TAATGCAATT 
TTCCCAAATG GAGCAGCATA TTGTATGGGA CGTATGAATT CTGACTGTTG GTACTTATAT 
ACTCTGGATT TCCCAGAGAG TCGGGTAATC AGTCAGCCAG ATCAAACCTT GGAAATTCTG 
ATGAGTGAGC TTGACCCAGC AGTTATGGAC CAGTTCTACA TGAAAGATGG TGTTACTGCA 
AAGGATGTCA CTCGTGAGAG TGGAATTCGT GACCTGATAC CAGGTTCTGT CATTGATGCC 
ACAATGTTCA ATCCTTGTGG GTATTCGATG AATGGAATGA AATCGGATGG AACTTATTGG 
ACTATTCACA TCACTCCAGA ACCAGAATTT TCTTATGTTA GCTTTGAAAC AAACTTAAGT 
CAGACCTCCT ATGATGACCT GATCAGGAAA GTTGTAGAAG TCTTCAAGCC AGGAAAATTT 
GTGACCACCT TGTTTGTTAA TCAGAGTTCT AAATGTCGCA CAGTGCTTGC TTCGCCCCAG 
AAGATTGAAG GTTTTAAGCG TCTTGATTGC CAGAGTGCTA TGTTCAATGA TTACAATTTT 
GTTTTTACCA GTTTTGCTAA GAAGCAGCAA CAACAGCAGA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001625.2
CDS	323..1327
Translation	MEAAHFFEGTEKLLEVWFSRQQPDANQGSGDLRTIPRSEWDILLKDVQCSIISVTKTDKQEAYVLSESSMFVSKRRFILKTCGTTLLLKALVPLLKLARDYSGFDSIQSFFYSRKNFMKPSHQGYPHRNFQEEIEFLNAIFPNGAAYCMGRMNSDCWYLYTLDFPESRVISQPDQTLEILMSELDPAVMDQFYMKDGVTAKDVTRESGIRDLIPGSVIDATMFNPCGYSMNGMKSDGTYWTIHITPEPEFSYVSFETNLSQTSYDDLIRKVVEVFKPGKFVTTLFVNQSSKCRTVLASPQKIEGFKRLDCQSAMFNDYNFVFTSFAKKQQQQQS

Target ORF information:

RefSeq Version	NM_001634.5
Organism	Homo sapiens(human)
Definition	Homo sapiens adenosylmethionine decarboxylase 1 (AMD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001634.5

ORF Insert Sequence:

ATGGAAGCTG CACATTTTTT CGAAGGGACC GAGAAGCTGC TGGAGGTTTG GTTCTCCCGG 
CAGCAGCCCG ACGCAAACCA AGGATCTGGG GATCTTCGCA CTATCCCAAG ATCTGAGTGG 
GACATACTTT TGAAGGATGT GCAATGTTCA ATCATAAGTG TGACAAAAAC TGACAAGCAG 
GAAGCTTATG TACTCAGTGA GAGTAGCATG TTTGTCTCCA AGAGACGTTT CATTTTGAAG 
ACATGTGGTA CCACCCTCTT GCTGAAAGCA CTGGTTCCCC TGTTGAAGCT TGCTAGGGAT 
TACAGTGGGT TTGACTCAAT TCAAAGCTTC TTTTATTCTC GTAAGAATTT CATGAAGCCT 
TCTCACCAAG GGTACCCACA CCGGAATTTC CAGGAAGAAA TAGAGTTTCT TAATGCAATT 
TTCCCAAATG GAGCAGCATA TTGTATGGGA CGTATGAATT CTGACTGTTG GTACTTATAT 
ACTCTGGATT TCCCAGAGAG TCGGGTAATC AGTCAGCCAG ATCAAACCTT GGAAATTCTG 
ATGAGTGAGC TTGACCCAGC AGTTATGGAC CAGTTCTACA TGAAAGATGG TGTTACTGCA 
AAGGATGTCA CTCGTGAGAG TGGAATTCGT GACCTGATAC CAGGTTCTGT CATTGATGCC 
ACAATGTTCA ATCCTTGTGG GTATTCGATG AATGGAATGA AATCGGATGG AACTTATTGG 
ACTATTCACA TCACTCCAGA ACCAGAATTT TCTTATGTTA GCTTTGAAAC AAACTTAAGT 
CAGACCTCCT ATGATGACCT GATCAGGAAA GTTGTAGAAG TCTTCAAGCC AGGAAAATTT 
GTGACCACCT TGTTTGTTAA TCAGAGTTCT AAATGTCGCA CAGTGCTTGC TTCGCCCCAG 
AAGATTGAAG GTTTTAAGCG TCTTGATTGC CAGAGTGCTA TGTTCAATGA TTACAATTTT 
GTTTTTACCA GTTTTGCTAA GAAGCAGCAA CAACAGCAGA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic variants in RUNX3, AMD1 and MSRA in the methionine metabolic pathway and survival in nonsmall cell lung cancer patients.
International journal of cancer145(3)621-631(2019 08)
Chen K,Liu H,Liu Z,Luo S,Patz EF Jr,Moorman PG,Su L,Shen S,Christiani DC,Wei Q

Polyamine Regulator AMD1 Promotes Cell Migration in Epidermal Wound Healing.
The Journal of investigative dermatology138(12)2653-2665(2018 12)
Lim HK,Rahim AB,Leo VI,Das S,Lim TC,Uemura T,Igarashi K,Common J,Vardy LA

AMD1 mRNA employs ribosome stalling as a mechanism for molecular memory formation.
Nature553(7688)356-360(2018 01)
Yordanova MM,Loughran G,Zhdanov AV,Mariotti M,Kiniry SJ,O'Connor PBF,Andreev DE,Tzani I,Saffert P,Michel AM,Gladyshev VN,Papkovsky DB,Atkins JF,Baranov PV

mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer.
Nature547(7661)109-113(2017 07)
Zabala-Letona A,Arruabarrena-Aristorena A,Martín-Martín N,Fernandez-Ruiz S,Sutherland JD,Clasquin M,Tomas-Cortazar J,Jimenez J,Torres I,Quang P,Ximenez-Embun P,Bago R,Ugalde-Olano A,Loizaga-Iriarte A,Lacasa-Viscasillas I,Unda M,Torrano V,Cabrera D,van Liempd SM,Cendon Y,Castro E,Murray S,Revandkar A,Alimonti A,Zhang Y,Barnett A,Lein G,Pirman D,Cortazar AR,Arreal L,Prudkin L,Astobiza I,Valcarcel-Jimenez L,Zuñiga-García P,Fernandez-Dominguez I,Piva M,Caro-Maldonado A,Sánchez-Mosquera P,Castillo-Martín M,Serra V,Beraza N,Gentilella A,Thomas G,Azkargorta M,Elortza F,Farràs R,Olmos D,Efeyan A,Anguita J,Muñoz J,Falcón-Pérez JM,Barrio R,Macarulla T,Mato JM,Martinez-Chantar ML,Cordon-Cardo C,Aransay AM,Marks K,Baselga J,Tabernero J,Nuciforo P,Manning BD,Marjon K,Carracedo A

Regulated translation termination at the upstream open reading frame in s-adenosylmethionine decarboxylase mRNA.
The Journal of biological chemistry277(8)5988-94(2002 Feb)
Raney A,Law GL,Mize GJ,Morris DR

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

AMD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM