Alox12e cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alox12e
Entrez Gene ID	11685
Full Name	arachidonate lipoxygenase, epidermal
Synonyms	8-LOX,AW259591,Alox12-ps1,Alox12-ps2,Aloxe,e-LOX1
General protein information	Preferred Names arachidonate lipoxygenase, epidermal Names arachidonate 12-lipoxygenase, epidermal-type 12-LOX arachidonate 12-lipoxygenase, epidermal
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B3\|11 42.99 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006532035.2	XP_006532098.1	arachidonate 12-lipoxygenase, epidermal-type isoform X2
XM_006532035.3	XP_006532098.1	arachidonate 12-lipoxygenase, epidermal-type isoform X2
NM_145684.2	NP_663717.1	arachidonate 12-lipoxygenase, epidermal-type
NM_145684.1	NP_663717.1	arachidonate 12-lipoxygenase, epidermal-type
XM_006532034.3	XP_006532097.1	arachidonate 12-lipoxygenase, epidermal-type isoform X1
XM_006532034.4	XP_006532097.1	arachidonate 12-lipoxygenase, epidermal-type isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC455243	XP_003315386.2
Macaca mulatta (Rhesus monkey)	LOC713683	XP_001104023.1
Canis lupus familiaris (dog)	LOC607567	XP_849396.2
Mus musculus (house mouse)	Alox12e	NP_663717.1
Bos taurus (cattle)	ALOX12E	NP_001077001.1
Rattus norvegicus (Norway rat)	Alox12e	NP_001100484.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

Transcription Factor MafB Coordinates Epidermal Keratinocyte Differentiation.
Miyai M, Hamada M, Moriguchi T, Hiruma J, Kamitani-Kawamoto A, Watanabe H, Hara-Chikuma M, Takahashi K, Takahashi S, Kataoka K
The Journal of investigative dermatology136(9)1848-1857(2016 09)

The disintegrin/metalloproteinase Adam10 is essential for epidermal integrity and Notch-mediated signaling.
Weber S, Niessen MT, Prox J, Lüllmann-Rauch R, Schmitz A, Schwanbeck R, Blobel CP, Jorissen E, de Strooper B, Niessen CM, Saftig P
Development (Cambridge, England)138(3)495-505(2011 Feb)

Identification of quantitative trait loci that regulate obesity and serum lipid levels in MRL/MpJ x SJL/J inbred mice.
Srivastava AK, Mohan S, Masinde GL, Yu H, Baylink DJ
Journal of lipid research47(1)123-33(2006 Jan)

Identification and characterization of a phorbol ester-responsive element in the murine 8S-lipoxygenase gene.
Kim E, Muga SJ, Fischer SM
The Journal of biological chemistry279(12)11188-97(2004 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Alox12e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alox12e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11940	NM_145684.2 Latest version!	Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu45438	XM_006532034.4 Latest version!	Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu45439	XM_006532035.3 Latest version!	Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu45439	XM_006532035.2	Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu11940	NM_145684.1	Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu45438	XM_006532034.3	Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alox12e ( NM_145684.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663717.1 Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), mRNA.

Price & Availability↑

CloneID	OMu11940
Clone ID Related Accession (Same CDS sequence)	NM_145684.2 , NM_145684.1
Accession Version	NM_145684.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Mus musculus(house mouse)
Product	arachidonate 12-lipoxygenase, epidermal-type
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL596096.7, AK138743.1 and BX630350.1. On Feb 13, 2018 this sequence version replaced NM_145684.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051047.1, X99252.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCAAGT ACAAGATCCT CGTAGCCACT GGAGACTCGG TTTTTGCGGG CTCCGCCAAC 
CTGGTGCATC TGTGGCTGGT GGGCGAGCAT GGAGAGGCGG ACCTTGGGAA GCAGCTGCGG 
CCACTGCTGG GAAGGAAGAC AGAGCTGGAG GTCGACGTCC CCTTGCATCT AGGGCGCCTC 
CTGGCGGTGA AGCTGCGCAA ACAGAAAGGC CTATTGGATT CTGACTGGTT CTGCAAGAGC 
ATCACTGTGC AGGGCCCTGG GACCCAAGGA GAAGCCTTTT TCCCCTGCTA CAGTTGGGTG 
CAGGGCAAGG AGACTATCTG CCTAACCGAG GGCACTGCCC TGAAGGTAAC TGATGACACT 
CAGAACCTGT TTAGGAAGTA TCGAGAGCAG GAGCTGGAGA ACAGAAGGAA TGTGTATCGG 
TGGGGCTCCT GGAAGGAGGG ATTAATCCTG CCTATAGCAG GGAGTACTGA ACGGGACCTC 
CCCCGGAACC AGAGATTCAT GAAGGATAAG GATTTAGATT TTTCCCTCTC TCTGGTCAAA 
GAGTTGAAGA ACTTTGCTAT TAAGGGGACT CTAGATTTTG TAAGTCGTGT ACAAAAGCTG 
GAAGATTACC AGAAAGTATT CCCACATACA AAGACTGCTC TGCCTGAGCG GGTCCGTGGT 
TCCTGGAAGG AAGATGCTCT CTTTGGGTAC CAGTTCCTCA ACGGTGCAAA CCCCATGCTC 
CTGAGGCGGT CCATGCGCCT TCCAGCCCGG CTGGTCCTGC CTCCAGGGAT GGAAGACGTA 
CAGACCCAGC TGGAGAAGGA GCTCAAGGCT GGATCTCTGT TCGAAGTTGA TTTCTCCCTG 
CTGGATGGAG TCAAACCTAA CATCATCATT TTTAAGCAGC AATATGTGAC AGCCCCTTTG 
GTCATGCTGA AACTACAGCC CGATGGGAGG CTCTTACCCA TGGTCATCCA GCTCCAGCCA 
CCACGACACG GATGTCCCCC GCCTCTGCTC TTCTTGCCCT CAGATCCCCC CATGGCCTGG 
CTCTTGGCCA AGATCTGGGT CCGAAGCTCT GACTTCCAAC TGCACCAGTT ACAGTCACAT 
CTGCTGAGGG GACACCTCAT GGCCGAGGTT ATTTCTGTGG CCACAATGAG GAGTTTACCC 
AGCCTGCACC CCATATACAA GCTCCTTGCC CCCCACTTTC GATACACCAT GGAGATCAAC 
ACCCTGGCAC GGAATAATCT TGTCTCCGAA TGGGGAATTT TTGACCTGGT GGTGAGCACG 
GGGAGCGGAG GCCATGTGGA CATTCTCCAG AGAGCCACGT CGTGTTTGAC CTACCGCTCC 
TTTTGCCCTC CGGATGACTT GGCTGACCGT GGACTCGTGG GGGTGAAATC TTCTCTGTAT 
GCCCAGGATG CCCTCAGGCT GTGGGAGATC ATCAGTCGGT ATGTGGAAAG GATGGTTGAG 
CTTTTCTACA GGAGTGACAC CGATGTGAAG GAGGACCCGG AGCTGCAGGT CTGGTGCCGC 
GAGGTCACTG AGGTTGGACT GCTTGGAGCC CAGGACCGAG GGTTCCCCCT GTCCTTAGAG 
TCCCGGGCTG AGCTGTGCCG CTTTGTCGCT ATGTGCATCT TCACGTGCAC TGGTCAGCAC 
GCATCTACAC ATCTGGGTCA GCTGGACTGG TATGCCTGGA TCCCAAATGG CCCATGCACC 
ATGCGGAAGC CCCCACCCAT CTCCAAGGAT GTGACAGAGA GGGACATTGT GGACTCGTTG 
CCTTGCCTTC AGCAGGCACG GATGCAGATA ACAGTCACCA AGTTCCTTGG CAGACGCCAG 
CCTGTCATGG TGGCCCTGGG GCAGCATAAA GAGGAGTATT TCTCAGGCCC CAGGCCCCGG 
GATGTGCTGA AGCAATTCCA GGAGGAGCTG GCTATCATGG ACAAGGAGAT TGAGGTCCGG 
AATGCAAGCC TGGACCTGCC CTATGAGTAC CTCCGACCCA GCCTGGTGGA AAACAGTGTG 
ACCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663717.1
CDS	75..2063
Translation	MVKYKILVATGDSVFAGSANLVHLWLVGEHGEADLGKQLRPLLGRKTELEVDVPLHLGRLLAVKLRKQKGLLDSDWFCKSITVQGPGTQGEAFFPCYSWVQGKETICLTEGTALKVTDDTQNLFRKYREQELENRRNVYRWGSWKEGLILPIAGSTERDLPRNQRFMKDKDLDFSLSLVKELKNFAIKGTLDFVSRVQKLEDYQKVFPHTKTALPERVRGSWKEDALFGYQFLNGANPMLLRRSMRLPARLVLPPGMEDVQTQLEKELKAGSLFEVDFSLLDGVKPNIIIFKQQYVTAPLVMLKLQPDGRLLPMVIQLQPPRHGCPPPLLFLPSDPPMAWLLAKIWVRSSDFQLHQLQSHLLRGHLMAEVISVATMRSLPSLHPIYKLLAPHFRYTMEINTLARNNLVSEWGIFDLVVSTGSGGHVDILQRATSCLTYRSFCPPDDLADRGLVGVKSSLYAQDALRLWEIISRYVERMVELFYRSDTDVKEDPELQVWCREVTEVGLLGAQDRGFPLSLESRAELCRFVAMCIFTCTGQHASTHLGQLDWYAWIPNGPCTMRKPPPISKDVTERDIVDSLPCLQQARMQITVTKFLGRRQPVMVALGQHKEEYFSGPRPRDVLKQFQEELAIMDKEIEVRNASLDLPYEYLRPSLVENSVTI

Target ORF information:

RefSeq Version	NM_145684.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145684.2

ORF Insert Sequence:

ATGGTCAAGT ACAAGATCCT CGTAGCCACT GGAGACTCGG TTTTTGCGGG CTCCGCCAAC 
CTGGTGCATC TGTGGCTGGT GGGCGAGCAT GGAGAGGCGG ACCTTGGGAA GCAGCTGCGG 
CCACTGCTGG GAAGGAAGAC AGAGCTGGAG GTCGACGTCC CCTTGCATCT AGGGCGCCTC 
CTGGCGGTGA AGCTGCGCAA ACAGAAAGGC CTATTGGATT CTGACTGGTT CTGCAAGAGC 
ATCACTGTGC AGGGCCCTGG GACCCAAGGA GAAGCCTTTT TCCCCTGCTA CAGTTGGGTG 
CAGGGCAAGG AGACTATCTG CCTAACCGAG GGCACTGCCC TGAAGGTAAC TGATGACACT 
CAGAACCTGT TTAGGAAGTA TCGAGAGCAG GAGCTGGAGA ACAGAAGGAA TGTGTATCGG 
TGGGGCTCCT GGAAGGAGGG ATTAATCCTG CCTATAGCAG GGAGTACTGA ACGGGACCTC 
CCCCGGAACC AGAGATTCAT GAAGGATAAG GATTTAGATT TTTCCCTCTC TCTGGTCAAA 
GAGTTGAAGA ACTTTGCTAT TAAGGGGACT CTAGATTTTG TAAGTCGTGT ACAAAAGCTG 
GAAGATTACC AGAAAGTATT CCCACATACA AAGACTGCTC TGCCTGAGCG GGTCCGTGGT 
TCCTGGAAGG AAGATGCTCT CTTTGGGTAC CAGTTCCTCA ACGGTGCAAA CCCCATGCTC 
CTGAGGCGGT CCATGCGCCT TCCAGCCCGG CTGGTCCTGC CTCCAGGGAT GGAAGACGTA 
CAGACCCAGC TGGAGAAGGA GCTCAAGGCT GGATCTCTGT TCGAAGTTGA TTTCTCCCTG 
CTGGATGGAG TCAAACCTAA CATCATCATT TTTAAGCAGC AATATGTGAC AGCCCCTTTG 
GTCATGCTGA AACTACAGCC CGATGGGAGG CTCTTACCCA TGGTCATCCA GCTCCAGCCA 
CCACGACACG GATGTCCCCC GCCTCTGCTC TTCTTGCCCT CAGATCCCCC CATGGCCTGG 
CTCTTGGCCA AGATCTGGGT CCGAAGCTCT GACTTCCAAC TGCACCAGTT ACAGTCACAT 
CTGCTGAGGG GACACCTCAT GGCCGAGGTT ATTTCTGTGG CCACAATGAG GAGTTTACCC 
AGCCTGCACC CCATATACAA GCTCCTTGCC CCCCACTTTC GATACACCAT GGAGATCAAC 
ACCCTGGCAC GGAATAATCT TGTCTCCGAA TGGGGAATTT TTGACCTGGT GGTGAGCACG 
GGGAGCGGAG GCCATGTGGA CATTCTCCAG AGAGCCACGT CGTGTTTGAC CTACCGCTCC 
TTTTGCCCTC CGGATGACTT GGCTGACCGT GGACTCGTGG GGGTGAAATC TTCTCTGTAT 
GCCCAGGATG CCCTCAGGCT GTGGGAGATC ATCAGTCGGT ATGTGGAAAG GATGGTTGAG 
CTTTTCTACA GGAGTGACAC CGATGTGAAG GAGGACCCGG AGCTGCAGGT CTGGTGCCGC 
GAGGTCACTG AGGTTGGACT GCTTGGAGCC CAGGACCGAG GGTTCCCCCT GTCCTTAGAG 
TCCCGGGCTG AGCTGTGCCG CTTTGTCGCT ATGTGCATCT TCACGTGCAC TGGTCAGCAC 
GCATCTACAC ATCTGGGTCA GCTGGACTGG TATGCCTGGA TCCCAAATGG CCCATGCACC 
ATGCGGAAGC CCCCACCCAT CTCCAAGGAT GTGACAGAGA GGGACATTGT GGACTCGTTG 
CCTTGCCTTC AGCAGGCACG GATGCAGATA ACAGTCACCA AGTTCCTTGG CAGACGCCAG 
CCTGTCATGG TGGCCCTGGG GCAGCATAAA GAGGAGTATT TCTCAGGCCC CAGGCCCCGG 
GATGTGCTGA AGCAATTCCA GGAGGAGCTG GCTATCATGG ACAAGGAGAT TGAGGTCCGG 
AATGCAAGCC TGGACCTGCC CTATGAGTAC CTCCGACCCA GCCTGGTGGA AAACAGTGTG 
ACCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alox12e ( NM_145684.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663717.1 Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), mRNA.

Price & Availability↑

CloneID	OMu11940
Clone ID Related Accession (Same CDS sequence)	NM_145684.2 , NM_145684.1
Accession Version	NM_145684.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509120000000
Organism	Mus musculus(house mouse)
Product	arachidonate 12-lipoxygenase, epidermal-type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X99252.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X99252.1, BC051047.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTCAAGT ACAAGATCCT CGTAGCCACT GGAGACTCGG TTTTTGCGGG CTCCGCCAAC 
CTGGTGCATC TGTGGCTGGT GGGCGAGCAT GGAGAGGCGG ACCTTGGGAA GCAGCTGCGG 
CCACTGCTGG GAAGGAAGAC AGAGCTGGAG GTCGACGTCC CCTTGCATCT AGGGCGCCTC 
CTGGCGGTGA AGCTGCGCAA ACAGAAAGGC CTATTGGATT CTGACTGGTT CTGCAAGAGC 
ATCACTGTGC AGGGCCCTGG GACCCAAGGA GAAGCCTTTT TCCCCTGCTA CAGTTGGGTG 
CAGGGCAAGG AGACTATCTG CCTAACCGAG GGCACTGCCC TGAAGGTAAC TGATGACACT 
CAGAACCTGT TTAGGAAGTA TCGAGAGCAG GAGCTGGAGA ACAGAAGGAA TGTGTATCGG 
TGGGGCTCCT GGAAGGAGGG ATTAATCCTG CCTATAGCAG GGAGTACTGA ACGGGACCTC 
CCCCGGAACC AGAGATTCAT GAAGGATAAG GATTTAGATT TTTCCCTCTC TCTGGTCAAA 
GAGTTGAAGA ACTTTGCTAT TAAGGGGACT CTAGATTTTG TAAGTCGTGT ACAAAAGCTG 
GAAGATTACC AGAAAGTATT CCCACATACA AAGACTGCTC TGCCTGAGCG GGTCCGTGGT 
TCCTGGAAGG AAGATGCTCT CTTTGGGTAC CAGTTCCTCA ACGGTGCAAA CCCCATGCTC 
CTGAGGCGGT CCATGCGCCT TCCAGCCCGG CTGGTCCTGC CTCCAGGGAT GGAAGACGTA 
CAGACCCAGC TGGAGAAGGA GCTCAAGGCT GGATCTCTGT TCGAAGTTGA TTTCTCCCTG 
CTGGATGGAG TCAAACCTAA CATCATCATT TTTAAGCAGC AATATGTGAC AGCCCCTTTG 
GTCATGCTGA AACTACAGCC CGATGGGAGG CTCTTACCCA TGGTCATCCA GCTCCAGCCA 
CCACGACACG GATGTCCCCC GCCTCTGCTC TTCTTGCCCT CAGATCCCCC CATGGCCTGG 
CTCTTGGCCA AGATCTGGGT CCGAAGCTCT GACTTCCAAC TGCACCAGTT ACAGTCACAT 
CTGCTGAGGG GACACCTCAT GGCCGAGGTT ATTTCTGTGG CCACAATGAG GAGTTTACCC 
AGCCTGCACC CCATATACAA GCTCCTTGCC CCCCACTTTC GATACACCAT GGAGATCAAC 
ACCCTGGCAC GGAATAATCT TGTCTCCGAA TGGGGAATTT TTGACCTGGT GGTGAGCACG 
GGGAGCGGAG GCCATGTGGA CATTCTCCAG AGAGCCACGT CGTGTTTGAC CTACCGCTCC 
TTTTGCCCTC CGGATGACTT GGCTGACCGT GGACTCGTGG GGGTGAAATC TTCTCTGTAT 
GCCCAGGATG CCCTCAGGCT GTGGGAGATC ATCAGTCGGT ATGTGGAAAG GATGGTTGAG 
CTTTTCTACA GGAGTGACAC CGATGTGAAG GAGGACCCGG AGCTGCAGGT CTGGTGCCGC 
GAGGTCACTG AGGTTGGACT GCTTGGAGCC CAGGACCGAG GGTTCCCCCT GTCCTTAGAG 
TCCCGGGCTG AGCTGTGCCG CTTTGTCGCT ATGTGCATCT TCACGTGCAC TGGTCAGCAC 
GCATCTACAC ATCTGGGTCA GCTGGACTGG TATGCCTGGA TCCCAAATGG CCCATGCACC 
ATGCGGAAGC CCCCACCCAT CTCCAAGGAT GTGACAGAGA GGGACATTGT GGACTCGTTG 
CCTTGCCTTC AGCAGGCACG GATGCAGATA ACAGTCACCA AGTTCCTTGG CAGACGCCAG 
CCTGTCATGG TGGCCCTGGG GCAGCATAAA GAGGAGTATT TCTCAGGCCC CAGGCCCCGG 
GATGTGCTGA AGCAATTCCA GGAGGAGCTG GCTATCATGG ACAAGGAGAT TGAGGTCCGG 
AATGCAAGCC TGGACCTGCC CTATGAGTAC CTCCGACCCA GCCTGGTGGA AAACAGTGTG 
ACCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663717.1
CDS	63..2051
Translation	MVKYKILVATGDSVFAGSANLVHLWLVGEHGEADLGKQLRPLLGRKTELEVDVPLHLGRLLAVKLRKQKGLLDSDWFCKSITVQGPGTQGEAFFPCYSWVQGKETICLTEGTALKVTDDTQNLFRKYREQELENRRNVYRWGSWKEGLILPIAGSTERDLPRNQRFMKDKDLDFSLSLVKELKNFAIKGTLDFVSRVQKLEDYQKVFPHTKTALPERVRGSWKEDALFGYQFLNGANPMLLRRSMRLPARLVLPPGMEDVQTQLEKELKAGSLFEVDFSLLDGVKPNIIIFKQQYVTAPLVMLKLQPDGRLLPMVIQLQPPRHGCPPPLLFLPSDPPMAWLLAKIWVRSSDFQLHQLQSHLLRGHLMAEVISVATMRSLPSLHPIYKLLAPHFRYTMEINTLARNNLVSEWGIFDLVVSTGSGGHVDILQRATSCLTYRSFCPPDDLADRGLVGVKSSLYAQDALRLWEIISRYVERMVELFYRSDTDVKEDPELQVWCREVTEVGLLGAQDRGFPLSLESRAELCRFVAMCIFTCTGQHASTHLGQLDWYAWIPNGPCTMRKPPPISKDVTERDIVDSLPCLQQARMQITVTKFLGRRQPVMVALGQHKEEYFSGPRPRDVLKQFQEELAIMDKEIEVRNASLDLPYEYLRPSLVENSVTI

Target ORF information:

RefSeq Version	NM_145684.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145684.1

ORF Insert Sequence:

ATGGTCAAGT ACAAGATCCT CGTAGCCACT GGAGACTCGG TTTTTGCGGG CTCCGCCAAC 
CTGGTGCATC TGTGGCTGGT GGGCGAGCAT GGAGAGGCGG ACCTTGGGAA GCAGCTGCGG 
CCACTGCTGG GAAGGAAGAC AGAGCTGGAG GTCGACGTCC CCTTGCATCT AGGGCGCCTC 
CTGGCGGTGA AGCTGCGCAA ACAGAAAGGC CTATTGGATT CTGACTGGTT CTGCAAGAGC 
ATCACTGTGC AGGGCCCTGG GACCCAAGGA GAAGCCTTTT TCCCCTGCTA CAGTTGGGTG 
CAGGGCAAGG AGACTATCTG CCTAACCGAG GGCACTGCCC TGAAGGTAAC TGATGACACT 
CAGAACCTGT TTAGGAAGTA TCGAGAGCAG GAGCTGGAGA ACAGAAGGAA TGTGTATCGG 
TGGGGCTCCT GGAAGGAGGG ATTAATCCTG CCTATAGCAG GGAGTACTGA ACGGGACCTC 
CCCCGGAACC AGAGATTCAT GAAGGATAAG GATTTAGATT TTTCCCTCTC TCTGGTCAAA 
GAGTTGAAGA ACTTTGCTAT TAAGGGGACT CTAGATTTTG TAAGTCGTGT ACAAAAGCTG 
GAAGATTACC AGAAAGTATT CCCACATACA AAGACTGCTC TGCCTGAGCG GGTCCGTGGT 
TCCTGGAAGG AAGATGCTCT CTTTGGGTAC CAGTTCCTCA ACGGTGCAAA CCCCATGCTC 
CTGAGGCGGT CCATGCGCCT TCCAGCCCGG CTGGTCCTGC CTCCAGGGAT GGAAGACGTA 
CAGACCCAGC TGGAGAAGGA GCTCAAGGCT GGATCTCTGT TCGAAGTTGA TTTCTCCCTG 
CTGGATGGAG TCAAACCTAA CATCATCATT TTTAAGCAGC AATATGTGAC AGCCCCTTTG 
GTCATGCTGA AACTACAGCC CGATGGGAGG CTCTTACCCA TGGTCATCCA GCTCCAGCCA 
CCACGACACG GATGTCCCCC GCCTCTGCTC TTCTTGCCCT CAGATCCCCC CATGGCCTGG 
CTCTTGGCCA AGATCTGGGT CCGAAGCTCT GACTTCCAAC TGCACCAGTT ACAGTCACAT 
CTGCTGAGGG GACACCTCAT GGCCGAGGTT ATTTCTGTGG CCACAATGAG GAGTTTACCC 
AGCCTGCACC CCATATACAA GCTCCTTGCC CCCCACTTTC GATACACCAT GGAGATCAAC 
ACCCTGGCAC GGAATAATCT TGTCTCCGAA TGGGGAATTT TTGACCTGGT GGTGAGCACG 
GGGAGCGGAG GCCATGTGGA CATTCTCCAG AGAGCCACGT CGTGTTTGAC CTACCGCTCC 
TTTTGCCCTC CGGATGACTT GGCTGACCGT GGACTCGTGG GGGTGAAATC TTCTCTGTAT 
GCCCAGGATG CCCTCAGGCT GTGGGAGATC ATCAGTCGGT ATGTGGAAAG GATGGTTGAG 
CTTTTCTACA GGAGTGACAC CGATGTGAAG GAGGACCCGG AGCTGCAGGT CTGGTGCCGC 
GAGGTCACTG AGGTTGGACT GCTTGGAGCC CAGGACCGAG GGTTCCCCCT GTCCTTAGAG 
TCCCGGGCTG AGCTGTGCCG CTTTGTCGCT ATGTGCATCT TCACGTGCAC TGGTCAGCAC 
GCATCTACAC ATCTGGGTCA GCTGGACTGG TATGCCTGGA TCCCAAATGG CCCATGCACC 
ATGCGGAAGC CCCCACCCAT CTCCAAGGAT GTGACAGAGA GGGACATTGT GGACTCGTTG 
CCTTGCCTTC AGCAGGCACG GATGCAGATA ACAGTCACCA AGTTCCTTGG CAGACGCCAG 
CCTGTCATGG TGGCCCTGGG GCAGCATAAA GAGGAGTATT TCTCAGGCCC CAGGCCCCGG 
GATGTGCTGA AGCAATTCCA GGAGGAGCTG GCTATCATGG ACAAGGAGAT TGAGGTCCGG 
AATGCAAGCC TGGACCTGCC CTATGAGTAC CTCCGACCCA GCCTGGTGGA AAACAGTGTG 
ACCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alox12e ( XM_006532034.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532097.1 Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), transcript variant X1, mRNA.

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CloneID	OMu45438
Clone ID Related Accession (Same CDS sequence)	XM_006532034.3 , XM_006532034.4
Accession Version	XM_006532034.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	arachidonate 12-lipoxygenase, epidermal-type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCT TGCCAAGAAA ACAAGGTAGG GATGCATTTT TGGAAACTGC TGGCAAACCA 
TCTAGAGACA GTGAAGGTCC CTCTAGAGGA TCAGCCGAGA TGGTCAAGTA CAAGATCCTC 
GTAGCCACTG GAGACTCGGT TTTTGCGGGC TCCGCCAACC TGGTGCATCT GTGGCTGGTG 
GGCGAGCATG GAGAGGCGGA CCTTGGGAAG CAGCTGCGGC CACTGCTGGG AAGGAAGACA 
GAGCTGGAGG TCGACGTCCC CTTGCATCTA GGGCGCCTCC TGGCGGTGAA GCTGCGCAAA 
CAGAAAGGCC TATTGGATTC TGACTGGTTC TGCAAGAGCA TCACTGTGCA GGGCCCTGGG 
ACCCAAGGAG AAGCCTTTTT CCCCTGCTAC AGTTGGGTGC AGGGCAAGGA GACTATCTGC 
CTAACCGAGG GCACTGCCCT GAAGGTAACT GATGACACTC AGAACCTGTT TAGGAAGTAT 
CGAGAGCAGG AGCTGGAGAA CAGAAGGAAT GTGTATCGGT GGGGCTCCTG GAAGGAGGGA 
TTAATCCTGC CTATAGCAGG GAGTACTGAA CGGGACCTCC CCCGGAACCA GAGATTCATG 
AAGGATAAGG ATTTAGATTT TTCCCTCTCT CTGGTCAAAG AGTTGAAGAA CTTTGCTATT 
AAGGGGACTC TAGATTTTGT AAGTCGTGTA CAAAAGCTGG AAGATTACCA GAAAGTATTC 
CCACATACAA AGACTGCTCT GCCTGAGCGG GTCCGTGGTT CCTGGAAGGA AGATGCTCTC 
TTTGGGTACC AGTTCCTCAA CGGTGCAAAC CCCATGCTCC TGAGGCGGTC CATGCGCCTT 
CCAGCCCGGC TGGTCCTGCC TCCAGGGATG GAAGACGTAC AGACCCAGCT GGAGAAGGAG 
CTCAAGGCTG GATCTCTGTT CGAAGTTGAT TTCTCCCTGC TGGATGGAGT CAAACCTAAC 
ATCATCATTT TTAAGCAGCA ATATGTGACA GCCCCTTTGG TCATGCTGAA ACTACAGCCC 
GATGGGAGGC TCTTACCCAT GGTCATCCAG CTCCAGCCAC CACGACACGG ATGTCCCCCG 
CCTCTGCTCT TCTTGCCCTC AGATCCCCCC ATGGCCTGGC TCTTGGCCAA GATCTGGGTC 
CGAAGCTCTG ACTTCCAACT GCACCAGTTA CAGTCACATC TGCTGAGGGG ACACCTCATG 
GCCGAGGTTA TTTCTGTGGC CACAATGAGG AGTTTACCCA GCCTGCACCC CATATACAAG 
CTCCTTGCCC CCCACTTTCG ATACACCATG GAGATCAACA CCCTGGCACG GAATAATCTT 
GTCTCCGAAT GGGGAATTTT TGACCTGGTG GTGAGCACGG GGAGCGGAGG CCATGTGGAC 
ATTCTCCAGA GAGCCACGTC GTGTTTGACC TACCGCTCCT TTTGCCCTCC GGATGACTTG 
GCTGACCGTG GACTCGTGGG GGTGAAATCT TCTCTGTATG CCCAGGATGC CCTCAGGCTG 
TGGGAGATCA TCAGTCGGTA TGTGGAAAGG ATGGTTGAGC TTTTCTACAG GAGTGACACC 
GATGTGAAGG AGGACCCGGA GCTGCAGGTC TGGTGCCGCG AGGTCACTGA GGTTGGACTG 
CTTGGAGCCC AGGACCGAGG GTTCCCCCTG TCCTTAGAGT CCCGGGCTGA GCTGTGCCGC 
TTTGTCGCTA TGTGCATCTT CACGTGCACT GGTCAGCACG CATCTACACA TCTGGGTCAG 
CTGGACTGGT ATGCCTGGAT CCCAAATGGC CCATGCACCA TGCGGAAGCC CCCACCCATC 
TCCAAGGATG TGACAGAGAG GGACATTGTG GACTCGTTGC CTTGCCTTCA GCAGGCACGG 
ATGCAGATAA CAGTCACCAA GTTCCTTGGC AGACGCCAGC CTGTCATGGT GGCCCTGGGG 
CAGCATAAAG AGGAGTATTT CTCAGGCCCC AGGCCCCGGG ATGTGCTGAA GCAATTCCAG 
GAGGAGCTGG CTATCATGGA CAAGGAGATT GAGGTCCGGA ATGCAAGCCT GGACCTGCCC 
TATGAGTACC TCCGACCCAG CCTGGTGGAA AACAGTGTGA CCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532097.1
CDS	36..2123
Translation	MKPLPRKQGRDAFLETAGKPSRDSEGPSRGSAEMVKYKILVATGDSVFAGSANLVHLWLVGEHGEADLGKQLRPLLGRKTELEVDVPLHLGRLLAVKLRKQKGLLDSDWFCKSITVQGPGTQGEAFFPCYSWVQGKETICLTEGTALKVTDDTQNLFRKYREQELENRRNVYRWGSWKEGLILPIAGSTERDLPRNQRFMKDKDLDFSLSLVKELKNFAIKGTLDFVSRVQKLEDYQKVFPHTKTALPERVRGSWKEDALFGYQFLNGANPMLLRRSMRLPARLVLPPGMEDVQTQLEKELKAGSLFEVDFSLLDGVKPNIIIFKQQYVTAPLVMLKLQPDGRLLPMVIQLQPPRHGCPPPLLFLPSDPPMAWLLAKIWVRSSDFQLHQLQSHLLRGHLMAEVISVATMRSLPSLHPIYKLLAPHFRYTMEINTLARNNLVSEWGIFDLVVSTGSGGHVDILQRATSCLTYRSFCPPDDLADRGLVGVKSSLYAQDALRLWEIISRYVERMVELFYRSDTDVKEDPELQVWCREVTEVGLLGAQDRGFPLSLESRAELCRFVAMCIFTCTGQHASTHLGQLDWYAWIPNGPCTMRKPPPISKDVTERDIVDSLPCLQQARMQITVTKFLGRRQPVMVALGQHKEEYFSGPRPRDVLKQFQEELAIMDKEIEVRNASLDLPYEYLRPSLVENSVTI

Target ORF information:

RefSeq Version	XM_006532034.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532034.3

ORF Insert Sequence:

ATGAAGCCCT TGCCAAGAAA ACAAGGTAGG GATGCATTTT TGGAAACTGC TGGCAAACCA 
TCTAGAGACA GTGAAGGTCC CTCTAGAGGA TCAGCCGAGA TGGTCAAGTA CAAGATCCTC 
GTAGCCACTG GAGACTCGGT TTTTGCGGGC TCCGCCAACC TGGTGCATCT GTGGCTGGTG 
GGCGAGCATG GAGAGGCGGA CCTTGGGAAG CAGCTGCGGC CACTGCTGGG AAGGAAGACA 
GAGCTGGAGG TCGACGTCCC CTTGCATCTA GGGCGCCTCC TGGCGGTGAA GCTGCGCAAA 
CAGAAAGGCC TATTGGATTC TGACTGGTTC TGCAAGAGCA TCACTGTGCA GGGCCCTGGG 
ACCCAAGGAG AAGCCTTTTT CCCCTGCTAC AGTTGGGTGC AGGGCAAGGA GACTATCTGC 
CTAACCGAGG GCACTGCCCT GAAGGTAACT GATGACACTC AGAACCTGTT TAGGAAGTAT 
CGAGAGCAGG AGCTGGAGAA CAGAAGGAAT GTGTATCGGT GGGGCTCCTG GAAGGAGGGA 
TTAATCCTGC CTATAGCAGG GAGTACTGAA CGGGACCTCC CCCGGAACCA GAGATTCATG 
AAGGATAAGG ATTTAGATTT TTCCCTCTCT CTGGTCAAAG AGTTGAAGAA CTTTGCTATT 
AAGGGGACTC TAGATTTTGT AAGTCGTGTA CAAAAGCTGG AAGATTACCA GAAAGTATTC 
CCACATACAA AGACTGCTCT GCCTGAGCGG GTCCGTGGTT CCTGGAAGGA AGATGCTCTC 
TTTGGGTACC AGTTCCTCAA CGGTGCAAAC CCCATGCTCC TGAGGCGGTC CATGCGCCTT 
CCAGCCCGGC TGGTCCTGCC TCCAGGGATG GAAGACGTAC AGACCCAGCT GGAGAAGGAG 
CTCAAGGCTG GATCTCTGTT CGAAGTTGAT TTCTCCCTGC TGGATGGAGT CAAACCTAAC 
ATCATCATTT TTAAGCAGCA ATATGTGACA GCCCCTTTGG TCATGCTGAA ACTACAGCCC 
GATGGGAGGC TCTTACCCAT GGTCATCCAG CTCCAGCCAC CACGACACGG ATGTCCCCCG 
CCTCTGCTCT TCTTGCCCTC AGATCCCCCC ATGGCCTGGC TCTTGGCCAA GATCTGGGTC 
CGAAGCTCTG ACTTCCAACT GCACCAGTTA CAGTCACATC TGCTGAGGGG ACACCTCATG 
GCCGAGGTTA TTTCTGTGGC CACAATGAGG AGTTTACCCA GCCTGCACCC CATATACAAG 
CTCCTTGCCC CCCACTTTCG ATACACCATG GAGATCAACA CCCTGGCACG GAATAATCTT 
GTCTCCGAAT GGGGAATTTT TGACCTGGTG GTGAGCACGG GGAGCGGAGG CCATGTGGAC 
ATTCTCCAGA GAGCCACGTC GTGTTTGACC TACCGCTCCT TTTGCCCTCC GGATGACTTG 
GCTGACCGTG GACTCGTGGG GGTGAAATCT TCTCTGTATG CCCAGGATGC CCTCAGGCTG 
TGGGAGATCA TCAGTCGGTA TGTGGAAAGG ATGGTTGAGC TTTTCTACAG GAGTGACACC 
GATGTGAAGG AGGACCCGGA GCTGCAGGTC TGGTGCCGCG AGGTCACTGA GGTTGGACTG 
CTTGGAGCCC AGGACCGAGG GTTCCCCCTG TCCTTAGAGT CCCGGGCTGA GCTGTGCCGC 
TTTGTCGCTA TGTGCATCTT CACGTGCACT GGTCAGCACG CATCTACACA TCTGGGTCAG 
CTGGACTGGT ATGCCTGGAT CCCAAATGGC CCATGCACCA TGCGGAAGCC CCCACCCATC 
TCCAAGGATG TGACAGAGAG GGACATTGTG GACTCGTTGC CTTGCCTTCA GCAGGCACGG 
ATGCAGATAA CAGTCACCAA GTTCCTTGGC AGACGCCAGC CTGTCATGGT GGCCCTGGGG 
CAGCATAAAG AGGAGTATTT CTCAGGCCCC AGGCCCCGGG ATGTGCTGAA GCAATTCCAG 
GAGGAGCTGG CTATCATGGA CAAGGAGATT GAGGTCCGGA ATGCAAGCCT GGACCTGCCC 
TATGAGTACC TCCGACCCAG CCTGGTGGAA AACAGTGTGA CCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alox12e ( XM_006532034.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532097.1 Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45438
Clone ID Related Accession (Same CDS sequence)	XM_006532034.3 , XM_006532034.4
Accession Version	XM_006532034.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	arachidonate 12-lipoxygenase, epidermal-type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532034.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCT TGCCAAGAAA ACAAGGTAGG GATGCATTTT TGGAAACTGC TGGCAAACCA 
TCTAGAGACA GTGAAGGTCC CTCTAGAGGA TCAGCCGAGA TGGTCAAGTA CAAGATCCTC 
GTAGCCACTG GAGACTCGGT TTTTGCGGGC TCCGCCAACC TGGTGCATCT GTGGCTGGTG 
GGCGAGCATG GAGAGGCGGA CCTTGGGAAG CAGCTGCGGC CACTGCTGGG AAGGAAGACA 
GAGCTGGAGG TCGACGTCCC CTTGCATCTA GGGCGCCTCC TGGCGGTGAA GCTGCGCAAA 
CAGAAAGGCC TATTGGATTC TGACTGGTTC TGCAAGAGCA TCACTGTGCA GGGCCCTGGG 
ACCCAAGGAG AAGCCTTTTT CCCCTGCTAC AGTTGGGTGC AGGGCAAGGA GACTATCTGC 
CTAACCGAGG GCACTGCCCT GAAGGTAACT GATGACACTC AGAACCTGTT TAGGAAGTAT 
CGAGAGCAGG AGCTGGAGAA CAGAAGGAAT GTGTATCGGT GGGGCTCCTG GAAGGAGGGA 
TTAATCCTGC CTATAGCAGG GAGTACTGAA CGGGACCTCC CCCGGAACCA GAGATTCATG 
AAGGATAAGG ATTTAGATTT TTCCCTCTCT CTGGTCAAAG AGTTGAAGAA CTTTGCTATT 
AAGGGGACTC TAGATTTTGT AAGTCGTGTA CAAAAGCTGG AAGATTACCA GAAAGTATTC 
CCACATACAA AGACTGCTCT GCCTGAGCGG GTCCGTGGTT CCTGGAAGGA AGATGCTCTC 
TTTGGGTACC AGTTCCTCAA CGGTGCAAAC CCCATGCTCC TGAGGCGGTC CATGCGCCTT 
CCAGCCCGGC TGGTCCTGCC TCCAGGGATG GAAGACGTAC AGACCCAGCT GGAGAAGGAG 
CTCAAGGCTG GATCTCTGTT CGAAGTTGAT TTCTCCCTGC TGGATGGAGT CAAACCTAAC 
ATCATCATTT TTAAGCAGCA ATATGTGACA GCCCCTTTGG TCATGCTGAA ACTACAGCCC 
GATGGGAGGC TCTTACCCAT GGTCATCCAG CTCCAGCCAC CACGACACGG ATGTCCCCCG 
CCTCTGCTCT TCTTGCCCTC AGATCCCCCC ATGGCCTGGC TCTTGGCCAA GATCTGGGTC 
CGAAGCTCTG ACTTCCAACT GCACCAGTTA CAGTCACATC TGCTGAGGGG ACACCTCATG 
GCCGAGGTTA TTTCTGTGGC CACAATGAGG AGTTTACCCA GCCTGCACCC CATATACAAG 
CTCCTTGCCC CCCACTTTCG ATACACCATG GAGATCAACA CCCTGGCACG GAATAATCTT 
GTCTCCGAAT GGGGAATTTT TGACCTGGTG GTGAGCACGG GGAGCGGAGG CCATGTGGAC 
ATTCTCCAGA GAGCCACGTC GTGTTTGACC TACCGCTCCT TTTGCCCTCC GGATGACTTG 
GCTGACCGTG GACTCGTGGG GGTGAAATCT TCTCTGTATG CCCAGGATGC CCTCAGGCTG 
TGGGAGATCA TCAGTCGGTA TGTGGAAAGG ATGGTTGAGC TTTTCTACAG GAGTGACACC 
GATGTGAAGG AGGACCCGGA GCTGCAGGTC TGGTGCCGCG AGGTCACTGA GGTTGGACTG 
CTTGGAGCCC AGGACCGAGG GTTCCCCCTG TCCTTAGAGT CCCGGGCTGA GCTGTGCCGC 
TTTGTCGCTA TGTGCATCTT CACGTGCACT GGTCAGCACG CATCTACACA TCTGGGTCAG 
CTGGACTGGT ATGCCTGGAT CCCAAATGGC CCATGCACCA TGCGGAAGCC CCCACCCATC 
TCCAAGGATG TGACAGAGAG GGACATTGTG GACTCGTTGC CTTGCCTTCA GCAGGCACGG 
ATGCAGATAA CAGTCACCAA GTTCCTTGGC AGACGCCAGC CTGTCATGGT GGCCCTGGGG 
CAGCATAAAG AGGAGTATTT CTCAGGCCCC AGGCCCCGGG ATGTGCTGAA GCAATTCCAG 
GAGGAGCTGG CTATCATGGA CAAGGAGATT GAGGTCCGGA ATGCAAGCCT GGACCTGCCC 
TATGAGTACC TCCGACCCAG CCTGGTGGAA AACAGTGTGA CCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532097.1
CDS	29..2116
Translation	MKPLPRKQGRDAFLETAGKPSRDSEGPSRGSAEMVKYKILVATGDSVFAGSANLVHLWLVGEHGEADLGKQLRPLLGRKTELEVDVPLHLGRLLAVKLRKQKGLLDSDWFCKSITVQGPGTQGEAFFPCYSWVQGKETICLTEGTALKVTDDTQNLFRKYREQELENRRNVYRWGSWKEGLILPIAGSTERDLPRNQRFMKDKDLDFSLSLVKELKNFAIKGTLDFVSRVQKLEDYQKVFPHTKTALPERVRGSWKEDALFGYQFLNGANPMLLRRSMRLPARLVLPPGMEDVQTQLEKELKAGSLFEVDFSLLDGVKPNIIIFKQQYVTAPLVMLKLQPDGRLLPMVIQLQPPRHGCPPPLLFLPSDPPMAWLLAKIWVRSSDFQLHQLQSHLLRGHLMAEVISVATMRSLPSLHPIYKLLAPHFRYTMEINTLARNNLVSEWGIFDLVVSTGSGGHVDILQRATSCLTYRSFCPPDDLADRGLVGVKSSLYAQDALRLWEIISRYVERMVELFYRSDTDVKEDPELQVWCREVTEVGLLGAQDRGFPLSLESRAELCRFVAMCIFTCTGQHASTHLGQLDWYAWIPNGPCTMRKPPPISKDVTERDIVDSLPCLQQARMQITVTKFLGRRQPVMVALGQHKEEYFSGPRPRDVLKQFQEELAIMDKEIEVRNASLDLPYEYLRPSLVENSVTI

Target ORF information:

RefSeq Version	XM_006532034.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532034.4

ORF Insert Sequence:

ATGAAGCCCT TGCCAAGAAA ACAAGGTAGG GATGCATTTT TGGAAACTGC TGGCAAACCA 
TCTAGAGACA GTGAAGGTCC CTCTAGAGGA TCAGCCGAGA TGGTCAAGTA CAAGATCCTC 
GTAGCCACTG GAGACTCGGT TTTTGCGGGC TCCGCCAACC TGGTGCATCT GTGGCTGGTG 
GGCGAGCATG GAGAGGCGGA CCTTGGGAAG CAGCTGCGGC CACTGCTGGG AAGGAAGACA 
GAGCTGGAGG TCGACGTCCC CTTGCATCTA GGGCGCCTCC TGGCGGTGAA GCTGCGCAAA 
CAGAAAGGCC TATTGGATTC TGACTGGTTC TGCAAGAGCA TCACTGTGCA GGGCCCTGGG 
ACCCAAGGAG AAGCCTTTTT CCCCTGCTAC AGTTGGGTGC AGGGCAAGGA GACTATCTGC 
CTAACCGAGG GCACTGCCCT GAAGGTAACT GATGACACTC AGAACCTGTT TAGGAAGTAT 
CGAGAGCAGG AGCTGGAGAA CAGAAGGAAT GTGTATCGGT GGGGCTCCTG GAAGGAGGGA 
TTAATCCTGC CTATAGCAGG GAGTACTGAA CGGGACCTCC CCCGGAACCA GAGATTCATG 
AAGGATAAGG ATTTAGATTT TTCCCTCTCT CTGGTCAAAG AGTTGAAGAA CTTTGCTATT 
AAGGGGACTC TAGATTTTGT AAGTCGTGTA CAAAAGCTGG AAGATTACCA GAAAGTATTC 
CCACATACAA AGACTGCTCT GCCTGAGCGG GTCCGTGGTT CCTGGAAGGA AGATGCTCTC 
TTTGGGTACC AGTTCCTCAA CGGTGCAAAC CCCATGCTCC TGAGGCGGTC CATGCGCCTT 
CCAGCCCGGC TGGTCCTGCC TCCAGGGATG GAAGACGTAC AGACCCAGCT GGAGAAGGAG 
CTCAAGGCTG GATCTCTGTT CGAAGTTGAT TTCTCCCTGC TGGATGGAGT CAAACCTAAC 
ATCATCATTT TTAAGCAGCA ATATGTGACA GCCCCTTTGG TCATGCTGAA ACTACAGCCC 
GATGGGAGGC TCTTACCCAT GGTCATCCAG CTCCAGCCAC CACGACACGG ATGTCCCCCG 
CCTCTGCTCT TCTTGCCCTC AGATCCCCCC ATGGCCTGGC TCTTGGCCAA GATCTGGGTC 
CGAAGCTCTG ACTTCCAACT GCACCAGTTA CAGTCACATC TGCTGAGGGG ACACCTCATG 
GCCGAGGTTA TTTCTGTGGC CACAATGAGG AGTTTACCCA GCCTGCACCC CATATACAAG 
CTCCTTGCCC CCCACTTTCG ATACACCATG GAGATCAACA CCCTGGCACG GAATAATCTT 
GTCTCCGAAT GGGGAATTTT TGACCTGGTG GTGAGCACGG GGAGCGGAGG CCATGTGGAC 
ATTCTCCAGA GAGCCACGTC GTGTTTGACC TACCGCTCCT TTTGCCCTCC GGATGACTTG 
GCTGACCGTG GACTCGTGGG GGTGAAATCT TCTCTGTATG CCCAGGATGC CCTCAGGCTG 
TGGGAGATCA TCAGTCGGTA TGTGGAAAGG ATGGTTGAGC TTTTCTACAG GAGTGACACC 
GATGTGAAGG AGGACCCGGA GCTGCAGGTC TGGTGCCGCG AGGTCACTGA GGTTGGACTG 
CTTGGAGCCC AGGACCGAGG GTTCCCCCTG TCCTTAGAGT CCCGGGCTGA GCTGTGCCGC 
TTTGTCGCTA TGTGCATCTT CACGTGCACT GGTCAGCACG CATCTACACA TCTGGGTCAG 
CTGGACTGGT ATGCCTGGAT CCCAAATGGC CCATGCACCA TGCGGAAGCC CCCACCCATC 
TCCAAGGATG TGACAGAGAG GGACATTGTG GACTCGTTGC CTTGCCTTCA GCAGGCACGG 
ATGCAGATAA CAGTCACCAA GTTCCTTGGC AGACGCCAGC CTGTCATGGT GGCCCTGGGG 
CAGCATAAAG AGGAGTATTT CTCAGGCCCC AGGCCCCGGG ATGTGCTGAA GCAATTCCAG 
GAGGAGCTGG CTATCATGGA CAAGGAGATT GAGGTCCGGA ATGCAAGCCT GGACCTGCCC 
TATGAGTACC TCCGACCCAG CCTGGTGGAA AACAGTGTGA CCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alox12e ( XM_006532035.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532098.1 Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu45439
Clone ID Related Accession (Same CDS sequence)	XM_006532035.2 , XM_006532035.3
Accession Version	XM_006532035.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	arachidonate 12-lipoxygenase, epidermal-type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006532035.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCT TGCCAAGAAA ACAAGGTAGG GATGCATTTT TGGAAACTGC TGGCAAACCA 
TCTAGAGACA GTGAAGGTCC CTCTAGAGGA TCAGCCGAGA TGGTCAAGTA CAAGATCCTC 
GTAGCCACTG GAGACTCGGT TTTTGCGGGC TCCGCCAACC TGGTGCATCT GTGGCTGGTG 
GGCGAGCATG GAGAGGCGGA CCTTGGGAAG CAGCTGCGGC CACTGCTGGG AAGGACAGAG 
CTGGAGGTCG ACGTCCCCTT GCATCTAGGG CGCCTCCTGG CGGTGAAGCT GCGCAAACAG 
AAAGGCCTAT TGGATTCTGA CTGGTTCTGC AAGAGCATCA CTGTGCAGGG CCCTGGGACC 
CAAGGAGAAG CCTTTTTCCC CTGCTACAGT TGGGTGCAGG GCAAGGAGAC TATCTGCCTA 
ACCGAGGGCA CTGCCCTGAA GGTAACTGAT GACACTCAGA ACCTGTTTAG GAAGTATCGA 
GAGCAGGAGC TGGAGAACAG AAGGAATGTG TATCGGTGGG GCTCCTGGAA GGAGGGATTA 
ATCCTGCCTA TAGCAGGGAG TACTGAACGG GACCTCCCCC GGAACCAGAG ATTCATGAAG 
GATAAGGATT TAGATTTTTC CCTCTCTCTG GTCAAAGAGT TGAAGAACTT TGCTATTAAG 
GGGACTCTAG ATTTTGTAAG TCGTGTACAA AAGCTGGAAG ATTACCAGAA AGTATTCCCA 
CATACAAAGA CTGCTCTGCC TGAGCGGGTC CGTGGTTCCT GGAAGGAAGA TGCTCTCTTT 
GGGTACCAGT TCCTCAACGG TGCAAACCCC ATGCTCCTGA GGCGGTCCAT GCGCCTTCCA 
GCCCGGCTGG TCCTGCCTCC AGGGATGGAA GACGTACAGA CCCAGCTGGA GAAGGAGCTC 
AAGGCTGGAT CTCTGTTCGA AGTTGATTTC TCCCTGCTGG ATGGAGTCAA ACCTAACATC 
ATCATTTTTA AGCAGCAATA TGTGACAGCC CCTTTGGTCA TGCTGAAACT ACAGCCCGAT 
GGGAGGCTCT TACCCATGGT CATCCAGCTC CAGCCACCAC GACACGGATG TCCCCCGCCT 
CTGCTCTTCT TGCCCTCAGA TCCCCCCATG GCCTGGCTCT TGGCCAAGAT CTGGGTCCGA 
AGCTCTGACT TCCAACTGCA CCAGTTACAG TCACATCTGC TGAGGGGACA CCTCATGGCC 
GAGGTTATTT CTGTGGCCAC AATGAGGAGT TTACCCAGCC TGCACCCCAT ATACAAGCTC 
CTTGCCCCCC ACTTTCGATA CACCATGGAG ATCAACACCC TGGCACGGAA TAATCTTGTC 
TCCGAATGGG GAATTTTTGA CCTGGTGGTG AGCACGGGGA GCGGAGGCCA TGTGGACATT 
CTCCAGAGAG CCACGTCGTG TTTGACCTAC CGCTCCTTTT GCCCTCCGGA TGACTTGGCT 
GACCGTGGAC TCGTGGGGGT GAAATCTTCT CTGTATGCCC AGGATGCCCT CAGGCTGTGG 
GAGATCATCA GTCGGTATGT GGAAAGGATG GTTGAGCTTT TCTACAGGAG TGACACCGAT 
GTGAAGGAGG ACCCGGAGCT GCAGGTCTGG TGCCGCGAGG TCACTGAGGT TGGACTGCTT 
GGAGCCCAGG ACCGAGGGTT CCCCCTGTCC TTAGAGTCCC GGGCTGAGCT GTGCCGCTTT 
GTCGCTATGT GCATCTTCAC GTGCACTGGT CAGCACGCAT CTACACATCT GGGTCAGCTG 
GACTGGTATG CCTGGATCCC AAATGGCCCA TGCACCATGC GGAAGCCCCC ACCCATCTCC 
AAGGATGTGA CAGAGAGGGA CATTGTGGAC TCGTTGCCTT GCCTTCAGCA GGCACGGATG 
CAGATAACAG TCACCAAGTT CCTTGGCAGA CGCCAGCCTG TCATGGTGGC CCTGGGGCAG 
CATAAAGAGG AGTATTTCTC AGGCCCCAGG CCCCGGGATG TGCTGAAGCA ATTCCAGGAG 
GAGCTGGCTA TCATGGACAA GGAGATTGAG GTCCGGAATG CAAGCCTGGA CCTGCCCTAT 
GAGTACCTCC GACCCAGCCT GGTGGAAAAC AGTGTGACCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532098.1
CDS	36..2120
Translation	MKPLPRKQGRDAFLETAGKPSRDSEGPSRGSAEMVKYKILVATGDSVFAGSANLVHLWLVGEHGEADLGKQLRPLLGRTELEVDVPLHLGRLLAVKLRKQKGLLDSDWFCKSITVQGPGTQGEAFFPCYSWVQGKETICLTEGTALKVTDDTQNLFRKYREQELENRRNVYRWGSWKEGLILPIAGSTERDLPRNQRFMKDKDLDFSLSLVKELKNFAIKGTLDFVSRVQKLEDYQKVFPHTKTALPERVRGSWKEDALFGYQFLNGANPMLLRRSMRLPARLVLPPGMEDVQTQLEKELKAGSLFEVDFSLLDGVKPNIIIFKQQYVTAPLVMLKLQPDGRLLPMVIQLQPPRHGCPPPLLFLPSDPPMAWLLAKIWVRSSDFQLHQLQSHLLRGHLMAEVISVATMRSLPSLHPIYKLLAPHFRYTMEINTLARNNLVSEWGIFDLVVSTGSGGHVDILQRATSCLTYRSFCPPDDLADRGLVGVKSSLYAQDALRLWEIISRYVERMVELFYRSDTDVKEDPELQVWCREVTEVGLLGAQDRGFPLSLESRAELCRFVAMCIFTCTGQHASTHLGQLDWYAWIPNGPCTMRKPPPISKDVTERDIVDSLPCLQQARMQITVTKFLGRRQPVMVALGQHKEEYFSGPRPRDVLKQFQEELAIMDKEIEVRNASLDLPYEYLRPSLVENSVTI

Target ORF information:

RefSeq Version	XM_006532035.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532035.2

ORF Insert Sequence:

ATGAAGCCCT TGCCAAGAAA ACAAGGTAGG GATGCATTTT TGGAAACTGC TGGCAAACCA 
TCTAGAGACA GTGAAGGTCC CTCTAGAGGA TCAGCCGAGA TGGTCAAGTA CAAGATCCTC 
GTAGCCACTG GAGACTCGGT TTTTGCGGGC TCCGCCAACC TGGTGCATCT GTGGCTGGTG 
GGCGAGCATG GAGAGGCGGA CCTTGGGAAG CAGCTGCGGC CACTGCTGGG AAGGACAGAG 
CTGGAGGTCG ACGTCCCCTT GCATCTAGGG CGCCTCCTGG CGGTGAAGCT GCGCAAACAG 
AAAGGCCTAT TGGATTCTGA CTGGTTCTGC AAGAGCATCA CTGTGCAGGG CCCTGGGACC 
CAAGGAGAAG CCTTTTTCCC CTGCTACAGT TGGGTGCAGG GCAAGGAGAC TATCTGCCTA 
ACCGAGGGCA CTGCCCTGAA GGTAACTGAT GACACTCAGA ACCTGTTTAG GAAGTATCGA 
GAGCAGGAGC TGGAGAACAG AAGGAATGTG TATCGGTGGG GCTCCTGGAA GGAGGGATTA 
ATCCTGCCTA TAGCAGGGAG TACTGAACGG GACCTCCCCC GGAACCAGAG ATTCATGAAG 
GATAAGGATT TAGATTTTTC CCTCTCTCTG GTCAAAGAGT TGAAGAACTT TGCTATTAAG 
GGGACTCTAG ATTTTGTAAG TCGTGTACAA AAGCTGGAAG ATTACCAGAA AGTATTCCCA 
CATACAAAGA CTGCTCTGCC TGAGCGGGTC CGTGGTTCCT GGAAGGAAGA TGCTCTCTTT 
GGGTACCAGT TCCTCAACGG TGCAAACCCC ATGCTCCTGA GGCGGTCCAT GCGCCTTCCA 
GCCCGGCTGG TCCTGCCTCC AGGGATGGAA GACGTACAGA CCCAGCTGGA GAAGGAGCTC 
AAGGCTGGAT CTCTGTTCGA AGTTGATTTC TCCCTGCTGG ATGGAGTCAA ACCTAACATC 
ATCATTTTTA AGCAGCAATA TGTGACAGCC CCTTTGGTCA TGCTGAAACT ACAGCCCGAT 
GGGAGGCTCT TACCCATGGT CATCCAGCTC CAGCCACCAC GACACGGATG TCCCCCGCCT 
CTGCTCTTCT TGCCCTCAGA TCCCCCCATG GCCTGGCTCT TGGCCAAGAT CTGGGTCCGA 
AGCTCTGACT TCCAACTGCA CCAGTTACAG TCACATCTGC TGAGGGGACA CCTCATGGCC 
GAGGTTATTT CTGTGGCCAC AATGAGGAGT TTACCCAGCC TGCACCCCAT ATACAAGCTC 
CTTGCCCCCC ACTTTCGATA CACCATGGAG ATCAACACCC TGGCACGGAA TAATCTTGTC 
TCCGAATGGG GAATTTTTGA CCTGGTGGTG AGCACGGGGA GCGGAGGCCA TGTGGACATT 
CTCCAGAGAG CCACGTCGTG TTTGACCTAC CGCTCCTTTT GCCCTCCGGA TGACTTGGCT 
GACCGTGGAC TCGTGGGGGT GAAATCTTCT CTGTATGCCC AGGATGCCCT CAGGCTGTGG 
GAGATCATCA GTCGGTATGT GGAAAGGATG GTTGAGCTTT TCTACAGGAG TGACACCGAT 
GTGAAGGAGG ACCCGGAGCT GCAGGTCTGG TGCCGCGAGG TCACTGAGGT TGGACTGCTT 
GGAGCCCAGG ACCGAGGGTT CCCCCTGTCC TTAGAGTCCC GGGCTGAGCT GTGCCGCTTT 
GTCGCTATGT GCATCTTCAC GTGCACTGGT CAGCACGCAT CTACACATCT GGGTCAGCTG 
GACTGGTATG CCTGGATCCC AAATGGCCCA TGCACCATGC GGAAGCCCCC ACCCATCTCC 
AAGGATGTGA CAGAGAGGGA CATTGTGGAC TCGTTGCCTT GCCTTCAGCA GGCACGGATG 
CAGATAACAG TCACCAAGTT CCTTGGCAGA CGCCAGCCTG TCATGGTGGC CCTGGGGCAG 
CATAAAGAGG AGTATTTCTC AGGCCCCAGG CCCCGGGATG TGCTGAAGCA ATTCCAGGAG 
GAGCTGGCTA TCATGGACAA GGAGATTGAG GTCCGGAATG CAAGCCTGGA CCTGCCCTAT 
GAGTACCTCC GACCCAGCCT GGTGGAAAAC AGTGTGACCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alox12e ( XM_006532035.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532098.1 Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), transcript variant X2, mRNA.

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CloneID	OMu45439
Clone ID Related Accession (Same CDS sequence)	XM_006532035.2 , XM_006532035.3
Accession Version	XM_006532035.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	arachidonate 12-lipoxygenase, epidermal-type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532035.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCT TGCCAAGAAA ACAAGGTAGG GATGCATTTT TGGAAACTGC TGGCAAACCA 
TCTAGAGACA GTGAAGGTCC CTCTAGAGGA TCAGCCGAGA TGGTCAAGTA CAAGATCCTC 
GTAGCCACTG GAGACTCGGT TTTTGCGGGC TCCGCCAACC TGGTGCATCT GTGGCTGGTG 
GGCGAGCATG GAGAGGCGGA CCTTGGGAAG CAGCTGCGGC CACTGCTGGG AAGGACAGAG 
CTGGAGGTCG ACGTCCCCTT GCATCTAGGG CGCCTCCTGG CGGTGAAGCT GCGCAAACAG 
AAAGGCCTAT TGGATTCTGA CTGGTTCTGC AAGAGCATCA CTGTGCAGGG CCCTGGGACC 
CAAGGAGAAG CCTTTTTCCC CTGCTACAGT TGGGTGCAGG GCAAGGAGAC TATCTGCCTA 
ACCGAGGGCA CTGCCCTGAA GGTAACTGAT GACACTCAGA ACCTGTTTAG GAAGTATCGA 
GAGCAGGAGC TGGAGAACAG AAGGAATGTG TATCGGTGGG GCTCCTGGAA GGAGGGATTA 
ATCCTGCCTA TAGCAGGGAG TACTGAACGG GACCTCCCCC GGAACCAGAG ATTCATGAAG 
GATAAGGATT TAGATTTTTC CCTCTCTCTG GTCAAAGAGT TGAAGAACTT TGCTATTAAG 
GGGACTCTAG ATTTTGTAAG TCGTGTACAA AAGCTGGAAG ATTACCAGAA AGTATTCCCA 
CATACAAAGA CTGCTCTGCC TGAGCGGGTC CGTGGTTCCT GGAAGGAAGA TGCTCTCTTT 
GGGTACCAGT TCCTCAACGG TGCAAACCCC ATGCTCCTGA GGCGGTCCAT GCGCCTTCCA 
GCCCGGCTGG TCCTGCCTCC AGGGATGGAA GACGTACAGA CCCAGCTGGA GAAGGAGCTC 
AAGGCTGGAT CTCTGTTCGA AGTTGATTTC TCCCTGCTGG ATGGAGTCAA ACCTAACATC 
ATCATTTTTA AGCAGCAATA TGTGACAGCC CCTTTGGTCA TGCTGAAACT ACAGCCCGAT 
GGGAGGCTCT TACCCATGGT CATCCAGCTC CAGCCACCAC GACACGGATG TCCCCCGCCT 
CTGCTCTTCT TGCCCTCAGA TCCCCCCATG GCCTGGCTCT TGGCCAAGAT CTGGGTCCGA 
AGCTCTGACT TCCAACTGCA CCAGTTACAG TCACATCTGC TGAGGGGACA CCTCATGGCC 
GAGGTTATTT CTGTGGCCAC AATGAGGAGT TTACCCAGCC TGCACCCCAT ATACAAGCTC 
CTTGCCCCCC ACTTTCGATA CACCATGGAG ATCAACACCC TGGCACGGAA TAATCTTGTC 
TCCGAATGGG GAATTTTTGA CCTGGTGGTG AGCACGGGGA GCGGAGGCCA TGTGGACATT 
CTCCAGAGAG CCACGTCGTG TTTGACCTAC CGCTCCTTTT GCCCTCCGGA TGACTTGGCT 
GACCGTGGAC TCGTGGGGGT GAAATCTTCT CTGTATGCCC AGGATGCCCT CAGGCTGTGG 
GAGATCATCA GTCGGTATGT GGAAAGGATG GTTGAGCTTT TCTACAGGAG TGACACCGAT 
GTGAAGGAGG ACCCGGAGCT GCAGGTCTGG TGCCGCGAGG TCACTGAGGT TGGACTGCTT 
GGAGCCCAGG ACCGAGGGTT CCCCCTGTCC TTAGAGTCCC GGGCTGAGCT GTGCCGCTTT 
GTCGCTATGT GCATCTTCAC GTGCACTGGT CAGCACGCAT CTACACATCT GGGTCAGCTG 
GACTGGTATG CCTGGATCCC AAATGGCCCA TGCACCATGC GGAAGCCCCC ACCCATCTCC 
AAGGATGTGA CAGAGAGGGA CATTGTGGAC TCGTTGCCTT GCCTTCAGCA GGCACGGATG 
CAGATAACAG TCACCAAGTT CCTTGGCAGA CGCCAGCCTG TCATGGTGGC CCTGGGGCAG 
CATAAAGAGG AGTATTTCTC AGGCCCCAGG CCCCGGGATG TGCTGAAGCA ATTCCAGGAG 
GAGCTGGCTA TCATGGACAA GGAGATTGAG GTCCGGAATG CAAGCCTGGA CCTGCCCTAT 
GAGTACCTCC GACCCAGCCT GGTGGAAAAC AGTGTGACCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532098.1
CDS	29..2113
Translation	MKPLPRKQGRDAFLETAGKPSRDSEGPSRGSAEMVKYKILVATGDSVFAGSANLVHLWLVGEHGEADLGKQLRPLLGRTELEVDVPLHLGRLLAVKLRKQKGLLDSDWFCKSITVQGPGTQGEAFFPCYSWVQGKETICLTEGTALKVTDDTQNLFRKYREQELENRRNVYRWGSWKEGLILPIAGSTERDLPRNQRFMKDKDLDFSLSLVKELKNFAIKGTLDFVSRVQKLEDYQKVFPHTKTALPERVRGSWKEDALFGYQFLNGANPMLLRRSMRLPARLVLPPGMEDVQTQLEKELKAGSLFEVDFSLLDGVKPNIIIFKQQYVTAPLVMLKLQPDGRLLPMVIQLQPPRHGCPPPLLFLPSDPPMAWLLAKIWVRSSDFQLHQLQSHLLRGHLMAEVISVATMRSLPSLHPIYKLLAPHFRYTMEINTLARNNLVSEWGIFDLVVSTGSGGHVDILQRATSCLTYRSFCPPDDLADRGLVGVKSSLYAQDALRLWEIISRYVERMVELFYRSDTDVKEDPELQVWCREVTEVGLLGAQDRGFPLSLESRAELCRFVAMCIFTCTGQHASTHLGQLDWYAWIPNGPCTMRKPPPISKDVTERDIVDSLPCLQQARMQITVTKFLGRRQPVMVALGQHKEEYFSGPRPRDVLKQFQEELAIMDKEIEVRNASLDLPYEYLRPSLVENSVTI

Target ORF information:

RefSeq Version	XM_006532035.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus arachidonate lipoxygenase, epidermal (Alox12e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532035.3

ORF Insert Sequence:

ATGAAGCCCT TGCCAAGAAA ACAAGGTAGG GATGCATTTT TGGAAACTGC TGGCAAACCA 
TCTAGAGACA GTGAAGGTCC CTCTAGAGGA TCAGCCGAGA TGGTCAAGTA CAAGATCCTC 
GTAGCCACTG GAGACTCGGT TTTTGCGGGC TCCGCCAACC TGGTGCATCT GTGGCTGGTG 
GGCGAGCATG GAGAGGCGGA CCTTGGGAAG CAGCTGCGGC CACTGCTGGG AAGGACAGAG 
CTGGAGGTCG ACGTCCCCTT GCATCTAGGG CGCCTCCTGG CGGTGAAGCT GCGCAAACAG 
AAAGGCCTAT TGGATTCTGA CTGGTTCTGC AAGAGCATCA CTGTGCAGGG CCCTGGGACC 
CAAGGAGAAG CCTTTTTCCC CTGCTACAGT TGGGTGCAGG GCAAGGAGAC TATCTGCCTA 
ACCGAGGGCA CTGCCCTGAA GGTAACTGAT GACACTCAGA ACCTGTTTAG GAAGTATCGA 
GAGCAGGAGC TGGAGAACAG AAGGAATGTG TATCGGTGGG GCTCCTGGAA GGAGGGATTA 
ATCCTGCCTA TAGCAGGGAG TACTGAACGG GACCTCCCCC GGAACCAGAG ATTCATGAAG 
GATAAGGATT TAGATTTTTC CCTCTCTCTG GTCAAAGAGT TGAAGAACTT TGCTATTAAG 
GGGACTCTAG ATTTTGTAAG TCGTGTACAA AAGCTGGAAG ATTACCAGAA AGTATTCCCA 
CATACAAAGA CTGCTCTGCC TGAGCGGGTC CGTGGTTCCT GGAAGGAAGA TGCTCTCTTT 
GGGTACCAGT TCCTCAACGG TGCAAACCCC ATGCTCCTGA GGCGGTCCAT GCGCCTTCCA 
GCCCGGCTGG TCCTGCCTCC AGGGATGGAA GACGTACAGA CCCAGCTGGA GAAGGAGCTC 
AAGGCTGGAT CTCTGTTCGA AGTTGATTTC TCCCTGCTGG ATGGAGTCAA ACCTAACATC 
ATCATTTTTA AGCAGCAATA TGTGACAGCC CCTTTGGTCA TGCTGAAACT ACAGCCCGAT 
GGGAGGCTCT TACCCATGGT CATCCAGCTC CAGCCACCAC GACACGGATG TCCCCCGCCT 
CTGCTCTTCT TGCCCTCAGA TCCCCCCATG GCCTGGCTCT TGGCCAAGAT CTGGGTCCGA 
AGCTCTGACT TCCAACTGCA CCAGTTACAG TCACATCTGC TGAGGGGACA CCTCATGGCC 
GAGGTTATTT CTGTGGCCAC AATGAGGAGT TTACCCAGCC TGCACCCCAT ATACAAGCTC 
CTTGCCCCCC ACTTTCGATA CACCATGGAG ATCAACACCC TGGCACGGAA TAATCTTGTC 
TCCGAATGGG GAATTTTTGA CCTGGTGGTG AGCACGGGGA GCGGAGGCCA TGTGGACATT 
CTCCAGAGAG CCACGTCGTG TTTGACCTAC CGCTCCTTTT GCCCTCCGGA TGACTTGGCT 
GACCGTGGAC TCGTGGGGGT GAAATCTTCT CTGTATGCCC AGGATGCCCT CAGGCTGTGG 
GAGATCATCA GTCGGTATGT GGAAAGGATG GTTGAGCTTT TCTACAGGAG TGACACCGAT 
GTGAAGGAGG ACCCGGAGCT GCAGGTCTGG TGCCGCGAGG TCACTGAGGT TGGACTGCTT 
GGAGCCCAGG ACCGAGGGTT CCCCCTGTCC TTAGAGTCCC GGGCTGAGCT GTGCCGCTTT 
GTCGCTATGT GCATCTTCAC GTGCACTGGT CAGCACGCAT CTACACATCT GGGTCAGCTG 
GACTGGTATG CCTGGATCCC AAATGGCCCA TGCACCATGC GGAAGCCCCC ACCCATCTCC 
AAGGATGTGA CAGAGAGGGA CATTGTGGAC TCGTTGCCTT GCCTTCAGCA GGCACGGATG 
CAGATAACAG TCACCAAGTT CCTTGGCAGA CGCCAGCCTG TCATGGTGGC CCTGGGGCAG 
CATAAAGAGG AGTATTTCTC AGGCCCCAGG CCCCGGGATG TGCTGAAGCA ATTCCAGGAG 
GAGCTGGCTA TCATGGACAA GGAGATTGAG GTCCGGAATG CAAGCCTGGA CCTGCCCTAT 
GAGTACCTCC GACCCAGCCT GGTGGAAAAC AGTGTGACCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

Transcription Factor MafB Coordinates Epidermal Keratinocyte Differentiation.
The Journal of investigative dermatology136(9)1848-1857(2016 09)
Miyai M,Hamada M,Moriguchi T,Hiruma J,Kamitani-Kawamoto A,Watanabe H,Hara-Chikuma M,Takahashi K,Takahashi S,Kataoka K

The disintegrin/metalloproteinase Adam10 is essential for epidermal integrity and Notch-mediated signaling.
Development (Cambridge, England)138(3)495-505(2011 Feb)
Weber S,Niessen MT,Prox J,Lüllmann-Rauch R,Schmitz A,Schwanbeck R,Blobel CP,Jorissen E,de Strooper B,Niessen CM,Saftig P

Identification of quantitative trait loci that regulate obesity and serum lipid levels in MRL/MpJ x SJL/J inbred mice.
Journal of lipid research47(1)123-33(2006 Jan)
Srivastava AK,Mohan S,Masinde GL,Yu H,Baylink DJ

Identification and characterization of a phorbol ester-responsive element in the murine 8S-lipoxygenase gene.
The Journal of biological chemistry279(12)11188-97(2004 Mar)
Kim E,Muga SJ,Fischer SM

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Alox12e cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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