Cpa1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cpa1
Entrez Gene ID	109697
Full Name	carboxypeptidase A1, pancreatic
Synonyms	0910001L12Rik,Cpa
General protein information	Preferred Names carboxypeptidase A1, pancreatic Names carboxypeptidase A1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 A3.3\|6 12.52 cM
Summary	This gene encodes carboxypeptidase A, a zinc-dependent metalloprotease that cleaves peptide bonds at the C-terminus of protein substrates. The encoded preproprotein undergoes proteolytic activation to generate a mature, functional enzyme. This gene is expressed in pancreas, the encoded protein is a major component of digestive enzymes secreted by pancreas and plays an important role in the process of digestion. This gene is located in a cluster of related carboxypeptidase genes on chromosome 6. [provided by RefSeq, Jan 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_025350.3	NP_079626.2	carboxypeptidase A1 precursor
NM_025350.4	NP_079626.2	carboxypeptidase A1 preproprotein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CPA1	XP_005628437.1
Bos taurus (cattle)	CPA1	NP_777175.1
Mus musculus (house mouse)	Cpa1	NP_079626.2
Anopheles gambiae (African malaria mosquito)	CBPA1_ANOGA	XP_318615.2
Macaca mulatta (Rhesus monkey)	CPA1	XP_001096893.1
Rattus norvegicus (Norway rat)	Cpa1	NP_058694.2
Gallus gallus (chicken)	CPA1	NP_989915.1
Danio rerio (zebrafish)	cpa5	NP_954965.1
Drosophila melanogaster (fruit fly)	CG17633	NP_609310.2
Homo sapiens (human)	CPA1	NP_001859.1
Danio rerio (zebrafish)	cpa1	NP_001018318.1
Xenopus tropicalis (tropical clawed frog)	cpa1	NP_001107681.1
Pan troglodytes (chimpanzee)	CPA1	XP_003318844.1

Gene Ontology
Bibliography

Related articles in PubMed

The Pdx1-Bound Swi/Snf Chromatin Remodeling Complex Regulates Pancreatic Progenitor Cell Proliferation and Mature Islet β-Cell Function.
Spaeth JM, Liu JH, Peters D, Guo M, Osipovich AB, Mohammadi F, Roy N, Bhushan A, Magnuson MA, Hebrok M, Wright CVE, Stein R
Diabetes68(9)1806-1818(2019 Sep)

LATS1/2 suppress NFκB and aberrant EMT initiation to permit pancreatic progenitor differentiation.
Braitsch CM, Azizoglu DB, Htike Y, Barlow HR, Schnell U, Chaney CP, Carroll TJ, Stanger BZ, Cleaver O
PLoS biology17(7)e3000382(2019 07)

Human
Hegyi E, Sahin-Tóth M
Gut68(2)301-312(2019 02)

Mechanosignalling via integrins directs fate decisions of pancreatic progenitors.
Mamidi A, Prawiro C, Seymour PA, de Lichtenberg KH, Jackson A, Serup P, Semb H
Nature564(7734)114-118(2018 12)

Endocrine lineage biases arise in temporally distinct endocrine progenitors during pancreatic morphogenesis.
Scavuzzo MA, Hill MC, Chmielowiec J, Yang D, Teaw J, Sheng K, Kong Y, Bettini M, Zong C, Martin JF, Borowiak M
Nature communications9(1)3356(2018 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cpa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cpa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16582	NM_025350.4 Latest version!	Mus musculus carboxypeptidase A1, pancreatic (Cpa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$188.30 $269.00
View Old Accession Versions >>
OMu16582	NM_025350.3	Mus musculus carboxypeptidase A1, pancreatic (Cpa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cpa1 ( NM_025350.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079626.2 Mus musculus carboxypeptidase A1, pancreatic (Cpa1), mRNA.

Price & Availability↑

CloneID	OMu16582
Clone ID Related Accession (Same CDS sequence)	NM_025350.3 , NM_025350.4
Accession Version	NM_025350.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1423843200000
Organism	Mus musculus(House mouse)
Product	carboxypeptidase A1 precursor
Comment

ATGAAGAGGC TGCTGGTTCT GAGTGTGCTG CTGGCAGCAG TCTTCGGCAA TGAGAACTTT 
GTGGGGCACC AGGTTCTCCG AATCTCTGCC ACCGACGAAG CCCAGGTGCA GAAAGTGAGG 
GAGCTGGAGG AGTTGGAGCA CTTGAAGTTG GACTTCTGGC GGGACCCTGC CCGGGCTGGT 
CTCCCCATCG ATGTTCGAGT GCCCTTCCCC ACCATCCAGT CCGTGAAAGC ATTCTTGGAA 
TATCATGATA TTAGCTATGA GATCATGATC GAAGATGTGC AGTCTCTGCT CGACGAGGAG 
AAGCAGCAGA TGTCCGCCTT CCAGGCCAGG GCTCTGTCCA CCGATGCTTT CAATTATGCC 
ACCTACCATA CTCTGGATGA GATCTATGAA TTCATGGACC TGCTGGTTAC AGAGCACCCA 
CAGCTTGTAA GCAAGATCCA GATCGGCAGC ACCTTTGAAG GTCGTCCCAT CAATGTGCTG 
AAGTTCAGCA CTGGAGGGAC CAATCGCCCA GCAATCTGGA TTGACACTGG CATCCATTCC 
AGGGAGTGGG TCACTCAGGC CAGTGGGGTC TGGTTTGCAA AAAAGATCAC GAAGGACTAT 
GGCCAGGAAC CCACCCTCAC GGCCATTCTT GACAACATGG ACATCTTCCT GGAGATTGTA 
ACTAACCCTG ATGGTTTTGT CTACACCCAC AAAACGAATC GCATGTGGCG CAAAACTCGA 
TCACACACCG AGGGCTCCCT CTGCGTCGGG GTGGACCCCA ACAGGAACTG GGATGCTGCC 
TTTGGGATGC CCGGAGCTAG TAGCAACCCC TGCTCAGAAA CTTACCGTGG CAAGTTTCCC 
AACTCTGAAG TGGAGGTCAA GTCCATCGTG GACTTTGTGA CGAGCCACGG GAACATCAAG 
GCCTTCATCT CCATCCACAG CTACTCCCAG CTCCTTCTCT ACCCCTATGG CTACACATCA 
GAACCAGCTC CTGACAAGGA GGAGCTGGAT CAGCTAGCAA AATCTGCTGT GACAGCCTTG 
ACATCTCTAC ACGGGACCAA GTTCAAGTAT GGCAGTATCA TTGATACGAT CTATCAAGCC 
AGTGGGAGCA CTATTGACTG GACCTACAGC CAGGGCATCA AGTATTCCTT CACTTTTGAG 
CTGAGGGACA CTGGGCTCAG AGGTTTCCTG CTGCCTGCCT CCCAGATCAT CCCCACAGCG 
GAGGAGACAT GGCTGGCCCT TTTGACCATC ATGGACCACA CTGTCAAACA CCCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_079626.2

Translation

MKRLLVLSVLLAAVFGNENFVGHQVLRISATDEAQVQKVRELEELEHLKLDFWRDPARAGLPIDVRVPFPTIQSVKAFLEYHDISYEIMIEDVQSLLDEEKQQMSAFQARALSTDAFNYATYHTLDEIYEFMDLLVTEHPQLVSKIQIGSTFEGRPINVLKFSTGGTNRPAIWIDTGIHSREWVTQASGVWFAKKITKDYGQEPTLTAILDNMDIFLEIVTNPDGFVYTHKTNRMWRKTRSHTEGSLCVGVDPNRNWDAAFGMPGASSNPCSETYRGKFPNSEVEVKSIVDFVTSHGNIKAFISIHSYSQLLLYPYGYTSEPAPDKEELDQLAKSAVTALTSLHGTKFKYGSIIDTIYQASGSTIDWTYSQGIKYSFTFELRDTGLRGFLLPASQIIPTAEETWLALLTIMDHTVKHPY

Target ORF information:

RefSeq Version	NM_025350.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus carboxypeptidase A1, pancreatic (Cpa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025350.3

ORF Insert Sequence:

ATGAAGAGGC TGCTGGTTCT GAGTGTGCTG CTGGCAGCAG TCTTCGGCAA TGAGAACTTT 
GTGGGGCACC AGGTTCTCCG AATCTCTGCC ACCGACGAAG CCCAGGTGCA GAAAGTGAGG 
GAGCTGGAGG AGTTGGAGCA CTTGAAGTTG GACTTCTGGC GGGACCCTGC CCGGGCTGGT 
CTCCCCATCG ATGTTCGAGT GCCCTTCCCC ACCATCCAGT CCGTGAAAGC ATTCTTGGAA 
TATCATGATA TTAGCTATGA GATCATGATC GAAGATGTGC AGTCTCTGCT CGACGAGGAG 
AAGCAGCAGA TGTCCGCCTT CCAGGCCAGG GCTCTGTCCA CCGATGCTTT CAATTATGCC 
ACCTACCATA CTCTGGATGA GATCTATGAA TTCATGGACC TGCTGGTTAC AGAGCACCCA 
CAGCTTGTAA GCAAGATCCA GATCGGCAGC ACCTTTGAAG GTCGTCCCAT CAATGTGCTG 
AAGTTCAGCA CTGGAGGGAC CAATCGCCCA GCAATCTGGA TTGACACTGG CATCCATTCC 
AGGGAGTGGG TCACTCAGGC CAGTGGGGTC TGGTTTGCAA AAAAGATCAC GAAGGACTAT 
GGCCAGGAAC CCACCCTCAC GGCCATTCTT GACAACATGG ACATCTTCCT GGAGATTGTA 
ACTAACCCTG ATGGTTTTGT CTACACCCAC AAAACGAATC GCATGTGGCG CAAAACTCGA 
TCACACACCG AGGGCTCCCT CTGCGTCGGG GTGGACCCCA ACAGGAACTG GGATGCTGCC 
TTTGGGATGC CCGGAGCTAG TAGCAACCCC TGCTCAGAAA CTTACCGTGG CAAGTTTCCC 
AACTCTGAAG TGGAGGTCAA GTCCATCGTG GACTTTGTGA CGAGCCACGG GAACATCAAG 
GCCTTCATCT CCATCCACAG CTACTCCCAG CTCCTTCTCT ACCCCTATGG CTACACATCA 
GAACCAGCTC CTGACAAGGA GGAGCTGGAT CAGCTAGCAA AATCTGCTGT GACAGCCTTG 
ACATCTCTAC ACGGGACCAA GTTCAAGTAT GGCAGTATCA TTGATACGAT CTATCAAGCC 
AGTGGGAGCA CTATTGACTG GACCTACAGC CAGGGCATCA AGTATTCCTT CACTTTTGAG 
CTGAGGGACA CTGGGCTCAG AGGTTTCCTG CTGCCTGCCT CCCAGATCAT CCCCACAGCG 
GAGGAGACAT GGCTGGCCCT TTTGACCATC ATGGACCACA CTGTCAAACA CCCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cpa1 ( NM_025350.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079626.2 Mus musculus carboxypeptidase A1, pancreatic (Cpa1), mRNA.

Price & Availability↑

CloneID	OMu16582
Clone ID Related Accession (Same CDS sequence)	NM_025350.3 , NM_025350.4
Accession Version	NM_025350.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576425600000
Organism	Mus musculus(house mouse)
Product	carboxypeptidase A1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC052661.1 and AC155656.10. On Jan 23, 2016 this sequence version replaced NM_025350.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052661.1, AK003088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGAGGC TGCTGGTTCT GAGTGTGCTG CTGGCAGCAG TCTTCGGCAA TGAGAACTTT 
GTGGGGCACC AGGTTCTCCG AATCTCTGCC ACCGACGAAG CCCAGGTGCA GAAAGTGAGG 
GAGCTGGAGG AGTTGGAGCA CTTGAAGTTG GACTTCTGGC GGGACCCTGC CCGGGCTGGT 
CTCCCCATCG ATGTTCGAGT GCCCTTCCCC ACCATCCAGT CCGTGAAAGC ATTCTTGGAA 
TATCATGATA TTAGCTATGA GATCATGATC GAAGATGTGC AGTCTCTGCT CGACGAGGAG 
AAGCAGCAGA TGTCCGCCTT CCAGGCCAGG GCTCTGTCCA CCGATGCTTT CAATTATGCC 
ACCTACCATA CTCTGGATGA GATCTATGAA TTCATGGACC TGCTGGTTAC AGAGCACCCA 
CAGCTTGTAA GCAAGATCCA GATCGGCAGC ACCTTTGAAG GTCGTCCCAT CAATGTGCTG 
AAGTTCAGCA CTGGAGGGAC CAATCGCCCA GCAATCTGGA TTGACACTGG CATCCATTCC 
AGGGAGTGGG TCACTCAGGC CAGTGGGGTC TGGTTTGCAA AAAAGATCAC GAAGGACTAT 
GGCCAGGAAC CCACCCTCAC GGCCATTCTT GACAACATGG ACATCTTCCT GGAGATTGTA 
ACTAACCCTG ATGGTTTTGT CTACACCCAC AAAACGAATC GCATGTGGCG CAAAACTCGA 
TCACACACCG AGGGCTCCCT CTGCGTCGGG GTGGACCCCA ACAGGAACTG GGATGCTGCC 
TTTGGGATGC CCGGAGCTAG TAGCAACCCC TGCTCAGAAA CTTACCGTGG CAAGTTTCCC 
AACTCTGAAG TGGAGGTCAA GTCCATCGTG GACTTTGTGA CGAGCCACGG GAACATCAAG 
GCCTTCATCT CCATCCACAG CTACTCCCAG CTCCTTCTCT ACCCCTATGG CTACACATCA 
GAACCAGCTC CTGACAAGGA GGAGCTGGAT CAGCTAGCAA AATCTGCTGT GACAGCCTTG 
ACATCTCTAC ACGGGACCAA GTTCAAGTAT GGCAGTATCA TTGATACGAT CTATCAAGCC 
AGTGGGAGCA CTATTGACTG GACCTACAGC CAGGGCATCA AGTATTCCTT CACTTTTGAG 
CTGAGGGACA CTGGGCTCAG AGGTTTCCTG CTGCCTGCCT CCCAGATCAT CCCCACAGCG 
GAGGAGACAT GGCTGGCCCT TTTGACCATC ATGGACCACA CTGTCAAACA CCCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079626.2
CDS	247..1506
Translation	MKRLLVLSVLLAAVFGNENFVGHQVLRISATDEAQVQKVRELEELEHLKLDFWRDPARAGLPIDVRVPFPTIQSVKAFLEYHDISYEIMIEDVQSLLDEEKQQMSAFQARALSTDAFNYATYHTLDEIYEFMDLLVTEHPQLVSKIQIGSTFEGRPINVLKFSTGGTNRPAIWIDTGIHSREWVTQASGVWFAKKITKDYGQEPTLTAILDNMDIFLEIVTNPDGFVYTHKTNRMWRKTRSHTEGSLCVGVDPNRNWDAAFGMPGASSNPCSETYRGKFPNSEVEVKSIVDFVTSHGNIKAFISIHSYSQLLLYPYGYTSEPAPDKEELDQLAKSAVTALTSLHGTKFKYGSIIDTIYQASGSTIDWTYSQGIKYSFTFELRDTGLRGFLLPASQIIPTAEETWLALLTIMDHTVKHPY

Target ORF information:

RefSeq Version	NM_025350.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus carboxypeptidase A1, pancreatic (Cpa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025350.4

ORF Insert Sequence:

ATGAAGAGGC TGCTGGTTCT GAGTGTGCTG CTGGCAGCAG TCTTCGGCAA TGAGAACTTT 
GTGGGGCACC AGGTTCTCCG AATCTCTGCC ACCGACGAAG CCCAGGTGCA GAAAGTGAGG 
GAGCTGGAGG AGTTGGAGCA CTTGAAGTTG GACTTCTGGC GGGACCCTGC CCGGGCTGGT 
CTCCCCATCG ATGTTCGAGT GCCCTTCCCC ACCATCCAGT CCGTGAAAGC ATTCTTGGAA 
TATCATGATA TTAGCTATGA GATCATGATC GAAGATGTGC AGTCTCTGCT CGACGAGGAG 
AAGCAGCAGA TGTCCGCCTT CCAGGCCAGG GCTCTGTCCA CCGATGCTTT CAATTATGCC 
ACCTACCATA CTCTGGATGA GATCTATGAA TTCATGGACC TGCTGGTTAC AGAGCACCCA 
CAGCTTGTAA GCAAGATCCA GATCGGCAGC ACCTTTGAAG GTCGTCCCAT CAATGTGCTG 
AAGTTCAGCA CTGGAGGGAC CAATCGCCCA GCAATCTGGA TTGACACTGG CATCCATTCC 
AGGGAGTGGG TCACTCAGGC CAGTGGGGTC TGGTTTGCAA AAAAGATCAC GAAGGACTAT 
GGCCAGGAAC CCACCCTCAC GGCCATTCTT GACAACATGG ACATCTTCCT GGAGATTGTA 
ACTAACCCTG ATGGTTTTGT CTACACCCAC AAAACGAATC GCATGTGGCG CAAAACTCGA 
TCACACACCG AGGGCTCCCT CTGCGTCGGG GTGGACCCCA ACAGGAACTG GGATGCTGCC 
TTTGGGATGC CCGGAGCTAG TAGCAACCCC TGCTCAGAAA CTTACCGTGG CAAGTTTCCC 
AACTCTGAAG TGGAGGTCAA GTCCATCGTG GACTTTGTGA CGAGCCACGG GAACATCAAG 
GCCTTCATCT CCATCCACAG CTACTCCCAG CTCCTTCTCT ACCCCTATGG CTACACATCA 
GAACCAGCTC CTGACAAGGA GGAGCTGGAT CAGCTAGCAA AATCTGCTGT GACAGCCTTG 
ACATCTCTAC ACGGGACCAA GTTCAAGTAT GGCAGTATCA TTGATACGAT CTATCAAGCC 
AGTGGGAGCA CTATTGACTG GACCTACAGC CAGGGCATCA AGTATTCCTT CACTTTTGAG 
CTGAGGGACA CTGGGCTCAG AGGTTTCCTG CTGCCTGCCT CCCAGATCAT CCCCACAGCG 
GAGGAGACAT GGCTGGCCCT TTTGACCATC ATGGACCACA CTGTCAAACA CCCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Pdx1-Bound Swi/Snf Chromatin Remodeling Complex Regulates Pancreatic Progenitor Cell Proliferation and Mature Islet β-Cell Function.
Diabetes68(9)1806-1818(2019 Sep)
Spaeth JM,Liu JH,Peters D,Guo M,Osipovich AB,Mohammadi F,Roy N,Bhushan A,Magnuson MA,Hebrok M,Wright CVE,Stein R

LATS1/2 suppress NFκB and aberrant EMT initiation to permit pancreatic progenitor differentiation.
PLoS biology17(7)e3000382(2019 07)
Braitsch CM,Azizoglu DB,Htike Y,Barlow HR,Schnell U,Chaney CP,Carroll TJ,Stanger BZ,Cleaver O

Human
Gut68(2)301-312(2019 02)
Hegyi E,Sahin-Tóth M

Mechanosignalling via integrins directs fate decisions of pancreatic progenitors.
Nature564(7734)114-118(2018 12)
Mamidi A,Prawiro C,Seymour PA,de Lichtenberg KH,Jackson A,Serup P,Semb H

Endocrine lineage biases arise in temporally distinct endocrine progenitors during pancreatic morphogenesis.
Nature communications9(1)3356(2018 08)
Scavuzzo MA,Hill MC,Chmielowiec J,Yang D,Teaw J,Sheng K,Kong Y,Bettini M,Zong C,Martin JF,Borowiak M

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Cpa1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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