cpa5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cpa5
Entrez Gene ID	246092
Full Name	carboxypeptidase A5
Synonyms	cb1060,cpa,wu:fb68f03
General protein information	Preferred Names carboxypeptidase A5 Names carboxypeptidase A carbA
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199271.1	NP_954965.1	carboxypeptidase A precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CPA1	XP_005628437.1
Bos taurus (cattle)	CPA1	NP_777175.1
Mus musculus (house mouse)	Cpa1	NP_079626.2
Anopheles gambiae (African malaria mosquito)	CBPA1_ANOGA	XP_318615.2
Macaca mulatta (Rhesus monkey)	CPA1	XP_001096893.1
Rattus norvegicus (Norway rat)	Cpa1	NP_058694.2
Gallus gallus (chicken)	CPA1	NP_989915.1
Danio rerio (zebrafish)	cpa5	NP_954965.1
Drosophila melanogaster (fruit fly)	CG17633	NP_609310.2
Homo sapiens (human)	CPA1	NP_001859.1
Danio rerio (zebrafish)	cpa1	NP_001018318.1
Xenopus tropicalis (tropical clawed frog)	cpa1	NP_001107681.1
Pan troglodytes (chimpanzee)	CPA1	XP_003318844.1

Gene Ontology
Bibliography

Related articles in PubMed

A metabolic interplay coordinated by HLX regulates myeloid differentiation and AML through partly overlapping pathways.
Piragyte I, Clapes T, Polyzou A, Klein Geltink RI, Lefkopoulos S, Yin N, Cauchy P, Curtis JD, Klaeylé L, Langa X, Beckmann CCA, Wlodarski MW, Müller P, Van Essen D, Rambold A, Kapp FG, Mione M, Buescher JM, Pearce EL, Polyzos A, Trompouki E
Nature communications9(1)3090(2018 08)

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
Tang Q, Iyer S, Lobbardi R, Moore JC, Chen H, Lareau C, Hebert C, Shaw ML, Neftel C, Suva ML, Ceol CJ, Bernards A, Aryee M, Pinello L, Drummond IA, Langenau DM
The Journal of experimental medicine214(10)2875-2887(2017 Oct)

Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development.
Nissim S, Weeks O, Talbot JC, Hedgepeth JW, Wucherpfennig J, Schatzman-Bone S, Swinburne I, Cortes M, Alexa K, Megason S, North TE, Amacher SL, Goessling W
Developmental biology418(1)108-123(2016 10)

Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish.
Moore FE, Garcia EG, Lobbardi R, Jain E, Tang Q, Moore JC, Cortes M, Molodtsov A, Kasheta M, Luo CC, Garcia AJ, Mylvaganam R, Yoder JA, Blackburn JS, Sadreyev RI, Ceol CJ, North TE, Langenau DM
The Journal of experimental medicine213(6)979-92(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cpa5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cpa5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33060	NM_199271.1 Latest version!	Danio rerio carboxypeptidase A5 (cpa5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cpa5 ( NM_199271.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_954965.1 Danio rerio carboxypeptidase A5 (cpa5), mRNA.

Price & Availability↑

CloneID	ODa33060
Clone ID Related Accession (Same CDS sequence)	NM_199271.1
Accession Version	NM_199271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	carboxypeptidase A precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC056300.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056300.1, BC065991.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505380 [ECO:0000348] ##Evidence-Data-END##

ATGAAGAGGC TGCTGGTGCT GATCGCTCTG TTTGTGGCCG TCTTTGGCAA AAAGACCTTT 
GAGGGGGATC AGGTGTTTCG GATCACTCCA AAAGATGAAG CTCAGATTGA CCTCCTGAAG 
GAGCTCACTG AAGAGGGCGA ACATCTTGGC CTTAGTGTCT GGATGGAGCC CATTCTTGAG 
TCTCTGCCTC TGGATCTTCA TGTCCCCTTT CACAGTCTCC AGGCTGTCAG GGCGTTTCTG 
GCCTACAATC AGATCCCATA CCACATCATG ATCGAGAATG TCCAGGAACT GTTGGATGAT 
GAGCAACGTG ATATGGTGAA ATATCGTGGC CTTGCAAGAT CTACAGATGA CTTTGTGTAC 
ACCACCTACC ATGACCTGAA CTCTATCAAC TCATTCATGG ACATGCTTGT CGCAGAGAAC 
AGGAACATGG TCAGCAAAGT TGTGATTGGT CAGAGCTACG AGAAACGCCC CTTGAATGTC 
CTGAAGTTCA GCACTGGAGC AAACCGTCCT GGTATCTGGA TCGACACTGG TATCCACTCC 
AGAGAATGGG TCACCCAGGC TAGTGGAGTC TGGTTTGCCA AGAAGATCGT GAAAGACTAT 
GGACGTGACC CCGTCCTCAC TGACATCTTG AACAGCCATG ATATCTTCCT GGAGATTGTC 
ACCAACCCTG ACGGTTTCGT CTACACCCAC ACCAAAGACC GCATGTGGCG TAAAACCAGA 
AAGCCAAACC CTGGCTCCAG CTGCGTTGGA GTTGATCCCA ACAGGAACTG GGATGCTGGT 
TTTGGAGGTG GTGGTTCCAG CAACAACCCC TGCACTGAAA CCTACCGCGG CCCCAGTGCT 
CACTCTGAGC CCGAGGTGAA GGCCATTGTG GACTTTGTGA AGTCTCACGG CAAAATCAAG 
GCCTTTGTGT CTATCCACAG CTATTCCCAG ATGCTCCTCT ACCCATACGG ATACACATAT 
ACCGCCGCCA AGGACAAGGC TGAACTGCAT GAGGTCGCCA GGAAGGCCAT CACTTCACTG 
CAGTCATTGT ACAACACTCG CTACACCTAT GGAAGCATCA TTACCACCAT CTATCAAGCC 
AGTGGAGGCA CTATTGACTG GACCTACAAC CAGGGCATCA AGTACTCCTA CACCTTTGAG 
CTGAGAGACA CCGGTCGCTA TGGTTTTATT CTGCCAGCCA ATCAAATCGT CCCAACCGCC 
GAGGAGACCT GGCTCGCTCT GATGGCCATC ATGGAGCACA CCAAGAATAA CCCTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954965.1
CDS	24..1283
Translation	MKRLLVLIALFVAVFGKKTFEGDQVFRITPKDEAQIDLLKELTEEGEHLGLSVWMEPILESLPLDLHVPFHSLQAVRAFLAYNQIPYHIMIENVQELLDDEQRDMVKYRGLARSTDDFVYTTYHDLNSINSFMDMLVAENRNMVSKVVIGQSYEKRPLNVLKFSTGANRPGIWIDTGIHSREWVTQASGVWFAKKIVKDYGRDPVLTDILNSHDIFLEIVTNPDGFVYTHTKDRMWRKTRKPNPGSSCVGVDPNRNWDAGFGGGGSSNNPCTETYRGPSAHSEPEVKAIVDFVKSHGKIKAFVSIHSYSQMLLYPYGYTYTAAKDKAELHEVARKAITSLQSLYNTRYTYGSIITTIYQASGGTIDWTYNQGIKYSYTFELRDTGRYGFILPANQIVPTAEETWLALMAIMEHTKNNPY

Target ORF information:

RefSeq Version	NM_199271.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carboxypeptidase A5 (cpa5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199271.1

ORF Insert Sequence:

ATGAAGAGGC TGCTGGTGCT GATCGCTCTG TTTGTGGCCG TCTTTGGCAA AAAGACCTTT 
GAGGGGGATC AGGTGTTTCG GATCACTCCA AAAGATGAAG CTCAGATTGA CCTCCTGAAG 
GAGCTCACTG AAGAGGGCGA ACATCTTGGC CTTAGTGTCT GGATGGAGCC CATTCTTGAG 
TCTCTGCCTC TGGATCTTCA TGTCCCCTTT CACAGTCTCC AGGCTGTCAG GGCGTTTCTG 
GCCTACAATC AGATCCCATA CCACATCATG ATCGAGAATG TCCAGGAACT GTTGGATGAT 
GAGCAACGTG ATATGGTGAA ATATCGTGGC CTTGCAAGAT CTACAGATGA CTTTGTGTAC 
ACCACCTACC ATGACCTGAA CTCTATCAAC TCATTCATGG ACATGCTTGT CGCAGAGAAC 
AGGAACATGG TCAGCAAAGT TGTGATTGGT CAGAGCTACG AGAAACGCCC CTTGAATGTC 
CTGAAGTTCA GCACTGGAGC AAACCGTCCT GGTATCTGGA TCGACACTGG TATCCACTCC 
AGAGAATGGG TCACCCAGGC TAGTGGAGTC TGGTTTGCCA AGAAGATCGT GAAAGACTAT 
GGACGTGACC CCGTCCTCAC TGACATCTTG AACAGCCATG ATATCTTCCT GGAGATTGTC 
ACCAACCCTG ACGGTTTCGT CTACACCCAC ACCAAAGACC GCATGTGGCG TAAAACCAGA 
AAGCCAAACC CTGGCTCCAG CTGCGTTGGA GTTGATCCCA ACAGGAACTG GGATGCTGGT 
TTTGGAGGTG GTGGTTCCAG CAACAACCCC TGCACTGAAA CCTACCGCGG CCCCAGTGCT 
CACTCTGAGC CCGAGGTGAA GGCCATTGTG GACTTTGTGA AGTCTCACGG CAAAATCAAG 
GCCTTTGTGT CTATCCACAG CTATTCCCAG ATGCTCCTCT ACCCATACGG ATACACATAT 
ACCGCCGCCA AGGACAAGGC TGAACTGCAT GAGGTCGCCA GGAAGGCCAT CACTTCACTG 
CAGTCATTGT ACAACACTCG CTACACCTAT GGAAGCATCA TTACCACCAT CTATCAAGCC 
AGTGGAGGCA CTATTGACTG GACCTACAAC CAGGGCATCA AGTACTCCTA CACCTTTGAG 
CTGAGAGACA CCGGTCGCTA TGGTTTTATT CTGCCAGCCA ATCAAATCGT CCCAACCGCC 
GAGGAGACCT GGCTCGCTCT GATGGCCATC ATGGAGCACA CCAAGAATAA CCCTTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A metabolic interplay coordinated by HLX regulates myeloid differentiation and AML through partly overlapping pathways.
Nature communications9(1)3090(2018 08)
Piragyte I,Clapes T,Polyzou A,Klein Geltink RI,Lefkopoulos S,Yin N,Cauchy P,Curtis JD,Klaeylé L,Langa X,Beckmann CCA,Wlodarski MW,Müller P,Van Essen D,Rambold A,Kapp FG,Mione M,Buescher JM,Pearce EL,Polyzos A,Trompouki E

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
The Journal of experimental medicine214(10)2875-2887(2017 Oct)
Tang Q,Iyer S,Lobbardi R,Moore JC,Chen H,Lareau C,Hebert C,Shaw ML,Neftel C,Suva ML,Ceol CJ,Bernards A,Aryee M,Pinello L,Drummond IA,Langenau DM

Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development.
Developmental biology418(1)108-123(2016 10)
Nissim S,Weeks O,Talbot JC,Hedgepeth JW,Wucherpfennig J,Schatzman-Bone S,Swinburne I,Cortes M,Alexa K,Megason S,North TE,Amacher SL,Goessling W

Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish.
The Journal of experimental medicine213(6)979-92(2016 05)
Moore FE,Garcia EG,Lobbardi R,Jain E,Tang Q,Moore JC,Cortes M,Molodtsov A,Kasheta M,Luo CC,Garcia AJ,Mylvaganam R,Yoder JA,Blackburn JS,Sadreyev RI,Ceol CJ,North TE,Langenau DM

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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cpa5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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