Lgr4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lgr4
Entrez Gene ID	107515
Full Name	leucine-rich repeat-containing G protein-coupled receptor 4
Synonyms	9130225G07,A330106J01Rik,A930009A08Rik,Gpr48
General protein information	Preferred Names leucine-rich repeat-containing G protein-coupled receptor 4 Names leucine-rich repeat-containing G-protein coupled receptor 4 G protein-coupled receptor GPR48 G-protein coupled receptor 48
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_172671.2	NP_766259.2	leucine-rich repeat-containing G-protein coupled receptor 4 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LGR4	XP_005633820.1
Pan troglodytes (chimpanzee)	LGR4	XP_003313024.2
Homo sapiens (human)	LGR4	NP_060960.2
Rattus norvegicus (Norway rat)	Lgr4	NP_775450.1
Macaca mulatta (Rhesus monkey)	LGR4	XP_001090038.2
Bos taurus (cattle)	LGR4	NP_001192440.1
Mus musculus (house mouse)	Lgr4	NP_766259.2
Gallus gallus (chicken)	LGR4	XP_426162.3
Xenopus tropicalis (tropical clawed frog)	lgr4	NP_001119970.1
Danio rerio (zebrafish)	lgr4	XP_687184.4

Gene Ontology
Bibliography

Related articles in PubMed

Loss of Lgr4 inhibits differentiation, migration and apoptosis, and promotes proliferation in bone mesenchymal stem cells.
Sun P, Jia K, Zheng C, Zhu X, Li J, He L, Siwko S, Xue F, Liu M, Luo J
Journal of cellular physiology234(7)10855-10867(2019 Jul)

LGR4 protects hepatocytes from injury in mouse.
Li Z, Liu S, Lou J, Mulholland M, Zhang W
American journal of physiology. Gastrointestinal and liver physiology316(1)G123-G131(2019 01)

Myocardial-specific R-spondin3 drives proliferation of the coronary stems primarily through the Leucine Rich Repeat G Protein coupled receptor LGR4.
Da Silva F, Massa F, Motamedi FJ, Vidal V, Rocha AS, Gregoire EP, Cai CL, Wagner KD, Schedl A
Developmental biology441(1)42-51(2018 09)

Inhibition of Rspo-Lgr4 Facilitates Checkpoint Blockade Therapy by Switching Macrophage Polarization.
Tan B, Shi X, Zhang J, Qin J, Zhang N, Ren H, Qian M, Siwko S, Carmon K, Liu Q, Han H, Du B, Liu M
Cancer research78(17)4929-4942(2018 09)

RSPO2 inhibition of RNF43 and ZNRF3 governs limb development independently of LGR4/5/6.
Szenker-Ravi E, Altunoglu U, Leushacke M, Bosso-Lefèvre C, Khatoo M, Thi Tran H, Naert T, Noelanders R, Hajamohideen A, Beneteau C, de Sousa SB, Karaman B, Latypova X, Başaran S, Yücel EB, Tan TT, Vlaminck L, Nayak SS, Shukla A, Girisha KM, Le Caignec C, Soshnikova N, Uyguner ZO, Vleminckx K, Barker N, Kayserili H, Reversade B
Nature557(7706)564-569(2018 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lgr4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lgr4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22784	NM_172671.2 Latest version!	Mus musculus leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lgr4 ( NM_172671.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766259.2 Mus musculus leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4), mRNA.

Price & Availability↑

CloneID	OMu22784
Clone ID Related Accession (Same CDS sequence)	NM_172671.2
Accession Version	NM_172671.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574438400000
Organism	Mus musculus(house mouse)
Product	leucine-rich repeat-containing G-protein coupled receptor 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ093786.1, BE987673.1, CF164628.1, CN667965.1, AK033699.1, BU520309.1, CN456468.1, CA527305.1 and BE861913.1. On or before Feb 4, 2007 this sequence version replaced XM_917125.2, XM_355385.4, NM_172671.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGGCC CGCTGCGGCT GCTCTGCTTC TTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCACC TCTCTGCGCT GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGGAAGGG GTTGACGGCG GTACCGGAGG GGCTCAGCGC CTTCACCCAA 
GCACTGGATA TCAGTATGAA CAATATCACT CAGTTACCAG AAGATGCATT TAAGAATTTT 
CCTTTTCTAG AGGAGCTACA ACTGGCTGGT AACGACCTTT CTTTTATCCA CCCAAAAGCC 
TTGTCTGGGT TGAAAGAACT CAAAGTCCTA ACCCTCCAGA ACAATCAGTT GAAAACAGTA 
CCCAGTGAAG CCATTCGTGG ACTGAGTGCT TTGCAGTCTC TACGCTTAGA TGCCAACCAT 
ATTACCTCAG TCCCGGAGGA CAGTTTTGAA GGGCTCGTTC AGTTGCGGCA TCTGTGGCTG 
GATGACAACA TCTTGACGGA AGTGCCTGTG CGTCCGCTCA GCAACCTGCC AACCCTGCAG 
GCGCTGACCT TGGCTCTCAA CAACATCTCA AGCATCCCCG ACTTCGCATT CACCAACCTT 
TCAAGCTTGG TAGTGCTGCA TCTTCATAAC AATAAAATTA AAAGCCTCAG TCAACACTGT 
TTTGATGGAC TAGATAACCT GGAAACCCTG GACTTGAATT ATAATAACTT GGATGAATTT 
CCTCAGGCTA TTAAAGCCCT TCCCAGCCTT AAAGAGCTGG GATTTCACAG TAATTCTATT 
TCTGTTATCC CGGATGGAGC ATTTGCTGGT AATCCACTGC TAAGAACTAT CCATTTGTAT 
GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ACCTGTCTGA TCTGCATTCC 
TTAGTCATTC GTGGTGCAAG CCTGGTGCAG TGGTTCCCCA ATCTGGCCGG AACTGTCCAT 
CTGGAGAGTC TAACCTTGAC AGGGACAAAA ATAAGCAGCA TACCTGATGA TCTGTGCCAA 
AACCAAAAGA TGCTGCGGAC TCTGGACTTA TCTTATAACG ATATAAGAGA CCTTCCAAGT 
TTTAATGGTT GTCGTGCATT GGAAGAAATT TCATTGCAGC GTAATCAAAT CTCCCTGATA 
AAGGAAACTA CTTTTCAAGG CCTAACATCC CTAAGGATTC TAGATCTGAG TAGAAACCTG 
ATTCGTGAAA TTCACAGTGG AGCTTTTGCG AAGCTTGGGA CAATTACTAA CCTGGATGTG 
AGTTTCAATG AATTAACCTC ATTTCCTACG GAAGGCCTGA ATGGGCTCAA TCAACTTAAA 
CTTGTGGGTA ACTTCCAGCT GAAAGATGCC TTGGCAGCCA GAGACTTTGC CAATCTCAGG 
TCTCTATCAG TACCATATGC TTATCAGTGT TGTGCATTTT GGGGGTGTGA CTCTTATGCA 
AATTTAAACA CAGAAGATAA CAGCCCCCAA GACCACAGTG TGACAAAAGA GAAAGGTGCT 
ACAGATGCAG CAAATGCCAC CAGCACTGCT GAAAGTGAAG AACATAGCCA AATAATCATC 
CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG 
ATTCGCCTTA CAGTGTGGTT CATTTTCCTG GTCGCCTTGC TTTTCAACCT GCTTGTCATT 
TTAACAGTGT TTGCGTCTTG TTCATCACTG CCTGCCTCCA AGCTCTTCAT AGGCTTGATT 
TCTGTGTCTA ACTTACTCAT GGGCATCTAT ACTGGCATCC TTACTTTTCT TGATGCTGTG 
TCCTGGGGCC GATTTGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGCGG CTGCAAGGTA 
GCTGGGTCTC TGGCAGTCTT CTCCTCAGAG AGCGCCGTAT TCCTGTTAAC CCTGGCAGCC 
GTGGAAAGAA GCGTTTTTGC AAAGGATGTA ATGAAAAATG GGAAAAGCAG TCACCTCCGA 
CAGTTCCAGG TGGCTGCCCT CGTAGCTTTG CTGGGTGCTG CAATAGCAGG CTGCTTCCCC 
CTTTTTCACG GAGGGCAATA TTCTGCATCA CCCTTGTGCT TGCCATTTCC TACAGGAGAG 
ACACCATCAT TAGGATTCAC TGTGACCCTA GTGCTATTAA ACTCACTAGC ATTTTTATTG 
ATGGCCATTA TCTACACTAA ACTCTACTGC AACTTAGAGA AAGAAGACCC GTCAGAAAAC 
TCCCAGTCTA GCATGATTAA GCACGTTGCT TGGCTCATCT TCACAAACTG CATCTTCTTC 
TGCCCTGTTG CATTTTTCTC ATTCGCACCA TTGATCACGG CAATCTCCAT CAGCCCCGAG 
ATAATGAAGT CTGTTACGCT GATATTCTTC CCGCTGCCTG CTTGCCTGAA TCCAGTCCTG 
TACGTTTTCT TCAACCCAAA GTTTAAAGAC GACTGGAAGC TCCTGAAGCG GCGTGTCACC 
AGGAAACACG GATCAGTCTC AGTCTCCATC AGCAGCCAAG GCGGTTGTGG GGAACAGGAT 
TTCTACTACG ACTGTGGCAT GTATTCCCAC TTGCAGGGTA ACCTGACTGT CTGTGACTGC 
TGTGAGTCAT TTCTTCTGAC AAAACCAGTA TCGTGCAAAC ACTTAATAAA ATCGCACAGT 
TGTCCTGTAT TGACAGTGGC CTCTTGCCAG AGGCCAGAGG CCTACTGGTC TGATTGTGGC 
ACACAGTCGG CCCATTCTGA CTATGCAGAT GAAGAGGATT CCTTTGTCTC GGACAGCTCT 
GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTCGCGGATT CCCTCTGGTG 
CGCTATGCTT ATAATCTACC GAGAGTCAGA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766259.2
CDS	475..3330
Translation	MPGPLRLLCFFALGLLGSAGPSGAAPPLCAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALDISMNNITQLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNILTEVPVRPLSNLPTLQALTLALNNISSIPDFAFTNLSSLVVLHLHNNKIKSLSQHCFDGLDNLETLDLNYNNLDEFPQAIKALPSLKELGFHSNSISVIPDGAFAGNPLLRTIHLYDNPLSFVGNSAFHNLSDLHSLVIRGASLVQWFPNLAGTVHLESLTLTGTKISSIPDDLCQNQKMLRTLDLSYNDIRDLPSFNGCRALEEISLQRNQISLIKETTFQGLTSLRILDLSRNLIREIHSGAFAKLGTITNLDVSFNELTSFPTEGLNGLNQLKLVGNFQLKDALAARDFANLRSLSVPYAYQCCAFWGCDSYANLNTEDNSPQDHSVTKEKGATDAANATSTAESEEHSQIIIHCTPSTGAFKPCEYLLGSWMIRLTVWFIFLVALLFNLLVILTVFASCSSLPASKLFIGLISVSNLLMGIYTGILTFLDAVSWGRFAEFGIWWETGSGCKVAGSLAVFSSESAVFLLTLAAVERSVFAKDVMKNGKSSHLRQFQVAALVALLGAAIAGCFPLFHGGQYSASPLCLPFPTGETPSLGFTVTLVLLNSLAFLLMAIIYTKLYCNLEKEDPSENSQSSMIKHVAWLIFTNCIFFCPVAFFSFAPLITAISISPEIMKSVTLIFFPLPACLNPVLYVFFNPKFKDDWKLLKRRVTRKHGSVSVSISSQGGCGEQDFYYDCGMYSHLQGNLTVCDCCESFLLTKPVSCKHLIKSHSCPVLTVASCQRPEAYWSDCGTQSAHSDYADEEDSFVSDSSDQVQACGRACFYQSRGFPLVRYAYNLPRVRD

Target ORF information:

RefSeq Version	NM_172671.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172671.2

ORF Insert Sequence:

ATGCCGGGCC CGCTGCGGCT GCTCTGCTTC TTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCACC TCTCTGCGCT GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGGAAGGG GTTGACGGCG GTACCGGAGG GGCTCAGCGC CTTCACCCAA 
GCACTGGATA TCAGTATGAA CAATATCACT CAGTTACCAG AAGATGCATT TAAGAATTTT 
CCTTTTCTAG AGGAGCTACA ACTGGCTGGT AACGACCTTT CTTTTATCCA CCCAAAAGCC 
TTGTCTGGGT TGAAAGAACT CAAAGTCCTA ACCCTCCAGA ACAATCAGTT GAAAACAGTA 
CCCAGTGAAG CCATTCGTGG ACTGAGTGCT TTGCAGTCTC TACGCTTAGA TGCCAACCAT 
ATTACCTCAG TCCCGGAGGA CAGTTTTGAA GGGCTCGTTC AGTTGCGGCA TCTGTGGCTG 
GATGACAACA TCTTGACGGA AGTGCCTGTG CGTCCGCTCA GCAACCTGCC AACCCTGCAG 
GCGCTGACCT TGGCTCTCAA CAACATCTCA AGCATCCCCG ACTTCGCATT CACCAACCTT 
TCAAGCTTGG TAGTGCTGCA TCTTCATAAC AATAAAATTA AAAGCCTCAG TCAACACTGT 
TTTGATGGAC TAGATAACCT GGAAACCCTG GACTTGAATT ATAATAACTT GGATGAATTT 
CCTCAGGCTA TTAAAGCCCT TCCCAGCCTT AAAGAGCTGG GATTTCACAG TAATTCTATT 
TCTGTTATCC CGGATGGAGC ATTTGCTGGT AATCCACTGC TAAGAACTAT CCATTTGTAT 
GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ACCTGTCTGA TCTGCATTCC 
TTAGTCATTC GTGGTGCAAG CCTGGTGCAG TGGTTCCCCA ATCTGGCCGG AACTGTCCAT 
CTGGAGAGTC TAACCTTGAC AGGGACAAAA ATAAGCAGCA TACCTGATGA TCTGTGCCAA 
AACCAAAAGA TGCTGCGGAC TCTGGACTTA TCTTATAACG ATATAAGAGA CCTTCCAAGT 
TTTAATGGTT GTCGTGCATT GGAAGAAATT TCATTGCAGC GTAATCAAAT CTCCCTGATA 
AAGGAAACTA CTTTTCAAGG CCTAACATCC CTAAGGATTC TAGATCTGAG TAGAAACCTG 
ATTCGTGAAA TTCACAGTGG AGCTTTTGCG AAGCTTGGGA CAATTACTAA CCTGGATGTG 
AGTTTCAATG AATTAACCTC ATTTCCTACG GAAGGCCTGA ATGGGCTCAA TCAACTTAAA 
CTTGTGGGTA ACTTCCAGCT GAAAGATGCC TTGGCAGCCA GAGACTTTGC CAATCTCAGG 
TCTCTATCAG TACCATATGC TTATCAGTGT TGTGCATTTT GGGGGTGTGA CTCTTATGCA 
AATTTAAACA CAGAAGATAA CAGCCCCCAA GACCACAGTG TGACAAAAGA GAAAGGTGCT 
ACAGATGCAG CAAATGCCAC CAGCACTGCT GAAAGTGAAG AACATAGCCA AATAATCATC 
CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG 
ATTCGCCTTA CAGTGTGGTT CATTTTCCTG GTCGCCTTGC TTTTCAACCT GCTTGTCATT 
TTAACAGTGT TTGCGTCTTG TTCATCACTG CCTGCCTCCA AGCTCTTCAT AGGCTTGATT 
TCTGTGTCTA ACTTACTCAT GGGCATCTAT ACTGGCATCC TTACTTTTCT TGATGCTGTG 
TCCTGGGGCC GATTTGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGCGG CTGCAAGGTA 
GCTGGGTCTC TGGCAGTCTT CTCCTCAGAG AGCGCCGTAT TCCTGTTAAC CCTGGCAGCC 
GTGGAAAGAA GCGTTTTTGC AAAGGATGTA ATGAAAAATG GGAAAAGCAG TCACCTCCGA 
CAGTTCCAGG TGGCTGCCCT CGTAGCTTTG CTGGGTGCTG CAATAGCAGG CTGCTTCCCC 
CTTTTTCACG GAGGGCAATA TTCTGCATCA CCCTTGTGCT TGCCATTTCC TACAGGAGAG 
ACACCATCAT TAGGATTCAC TGTGACCCTA GTGCTATTAA ACTCACTAGC ATTTTTATTG 
ATGGCCATTA TCTACACTAA ACTCTACTGC AACTTAGAGA AAGAAGACCC GTCAGAAAAC 
TCCCAGTCTA GCATGATTAA GCACGTTGCT TGGCTCATCT TCACAAACTG CATCTTCTTC 
TGCCCTGTTG CATTTTTCTC ATTCGCACCA TTGATCACGG CAATCTCCAT CAGCCCCGAG 
ATAATGAAGT CTGTTACGCT GATATTCTTC CCGCTGCCTG CTTGCCTGAA TCCAGTCCTG 
TACGTTTTCT TCAACCCAAA GTTTAAAGAC GACTGGAAGC TCCTGAAGCG GCGTGTCACC 
AGGAAACACG GATCAGTCTC AGTCTCCATC AGCAGCCAAG GCGGTTGTGG GGAACAGGAT 
TTCTACTACG ACTGTGGCAT GTATTCCCAC TTGCAGGGTA ACCTGACTGT CTGTGACTGC 
TGTGAGTCAT TTCTTCTGAC AAAACCAGTA TCGTGCAAAC ACTTAATAAA ATCGCACAGT 
TGTCCTGTAT TGACAGTGGC CTCTTGCCAG AGGCCAGAGG CCTACTGGTC TGATTGTGGC 
ACACAGTCGG CCCATTCTGA CTATGCAGAT GAAGAGGATT CCTTTGTCTC GGACAGCTCT 
GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTCGCGGATT CCCTCTGGTG 
CGCTATGCTT ATAATCTACC GAGAGTCAGA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of Lgr4 inhibits differentiation, migration and apoptosis, and promotes proliferation in bone mesenchymal stem cells.
Journal of cellular physiology234(7)10855-10867(2019 Jul)
Sun P,Jia K,Zheng C,Zhu X,Li J,He L,Siwko S,Xue F,Liu M,Luo J

LGR4 protects hepatocytes from injury in mouse.
American journal of physiology. Gastrointestinal and liver physiology316(1)G123-G131(2019 01)
Li Z,Liu S,Lou J,Mulholland M,Zhang W

Myocardial-specific R-spondin3 drives proliferation of the coronary stems primarily through the Leucine Rich Repeat G Protein coupled receptor LGR4.
Developmental biology441(1)42-51(2018 09)
Da Silva F,Massa F,Motamedi FJ,Vidal V,Rocha AS,Gregoire EP,Cai CL,Wagner KD,Schedl A

Inhibition of Rspo-Lgr4 Facilitates Checkpoint Blockade Therapy by Switching Macrophage Polarization.
Cancer research78(17)4929-4942(2018 09)
Tan B,Shi X,Zhang J,Qin J,Zhang N,Ren H,Qian M,Siwko S,Carmon K,Liu Q,Han H,Du B,Liu M

RSPO2 inhibition of RNF43 and ZNRF3 governs limb development independently of LGR4/5/6.
Nature557(7706)564-569(2018 05)
Szenker-Ravi E,Altunoglu U,Leushacke M,Bosso-Lefèvre C,Khatoo M,Thi Tran H,Naert T,Noelanders R,Hajamohideen A,Beneteau C,de Sousa SB,Karaman B,Latypova X,Başaran S,Yücel EB,Tan TT,Vlaminck L,Nayak SS,Shukla A,Girisha KM,Le Caignec C,Soshnikova N,Uyguner ZO,Vleminckx K,Barker N,Kayserili H,Reversade B

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Lgr4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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