LMJF_32_3020 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_32_3020 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_32_3020 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb06617 XM_001685576.1
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Leishmania major strain Friedlin conserved hypothetical protein partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OLb06617
    Clone ID Related Accession (Same CDS sequence) XM_001685576.1
    Accession Version XM_001685576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product conserved hypothetical protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007273). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCTTTTG CTACTTCTAT TACCTGTGTC CTTTTCTACA TGCTGCAACT CTCTCTGCTT 
    CTTCGTGGTG CTCTGTGCAT TTCGTATCGC GCACTGAAAA GCGCCGCAGC CGCCCCTGTC
    GCCGCAGCGG GGGTGTCAGC CGCTAGCTCG AGATTAGGCG GAGCATGGGA GGGTAGTATC
    GAGCGCGCTG AGGCTCTCGA GAACCTCAGC AACGAGGTGT TTGGCCGAGG AAACAGACAT
    TTGAAAAATG TGGAAACGCT GACGGCTCTC CCGGAAGGTC TGCACTCCTT CCCAGAAGTG
    TGCTTCATTG GGAAGCCAGG GTGCGGCAAG TCGAGCCTTA TCTCGTGTCT TTTGCATAAT
    CACCGGCTGG GCAAAGCCGG GGCAACTGCC GGCACTACGC GACTTCTGCA ATTTTTCAAT
    GTGGGCGACG CGCTTCTCTT GGTGGATACA CCAGGCTACG GTGGCTGGCG AGGTCGTGAG
    GTGGGACAGC GTCTAGCAGA GCAGGCCAAC GCGTTTTCGA TTCTGTTCCG CTACCTGGCC
    TTGCGCAACG GGAGTAATCT CAAGCGAGTA TACTGGCTCA TGGAGTCCTC GGCGCCGACG
    CCAGTGTCCT TCCAACCGCG TGACGAGGAG CTTCTAACTT TTCTCTCCCG GGAGCGCATT
    CCTTTTAGTG TCGTGCTCAC CAAGATCGAC CGCCACTGGC GCCACTACGC CGAGCAGCAG
    CGGACGGCTG ACCGAGTAGG AAACGATGGC TTCGTACACC CAAGGCACAG AAGACCAGAA
    ACACACGATC AGGGCTTTTT TACGCGCACG AGCCTGCCTC AAGACGGTGT GGCACGCAAT
    ATGCAGGAGG TGTATGAGTT TCTAGGCACC GATCAAGTAC CAATTCTCGG CGTAAGTGCC
    AACCGCCTAC AACCAAACCG TTCGCGCAAT CTGGAGCTGC TGCAGCACGA CATCGTTCAC
    TACTGCACGC AAGACCTGCT GCACTCAGAG GAGCTCTCTT TTCGCAACAT GCACAAGCTG
    AGCTACGCAC CACCGACGGC TGATCGTATT CATGAGATTC AGCTGCGATA CCCAGTGGAG
    TCGTTCGTTG TTCCCGAGAA CAACAACATG TCATTGGCAC GCATGGTGGA GCACCACGAG
    GAGATGAAAG CACGATTTTG CGAAAACAAC GTATGTGCGC GGCGCCTCTC CGCCAAAGAT
    GTGGCGGCGT GCCATCTAGA GAGCGTCGCA AAGCTGAAGG CGGCCTGTAA ACACGATACT
    GCAGAACGAG AGCGCGCGGA AAAGGTGCGT CCCACCGCGA CCTATCTCTT CGAATCTACC
    TTGGCTGCCG CTGTAACGTC GCTGCCGCTG CCAGGCGATG TCGCAGGCCT GTTGCGCGCC
    AGCGAAGCGC AGGGCACCGC TACTGCATCG GTAAGCCGTG TAGGGGGCCT TCCTGAGCGA
    TGGAAGGAAT GCGATGACCA CAATGCCGAG GCGTGCGCGG CTGACGTGTG CGCTAGCCGG
    CGCTTCCGCG TGCGGCCGCT CCTCGTAGGC GAGAGGCAGA CGAGCCGAGA CTCCTTGGGG
    GATATACTGG AGCGTGCGCT CGATGAGGAG GAGCCGAGGC AGGACTTTGC AGGTAGCATC
    GTGGACAAAG GCGAAAAGAC CGCCAACGCA GCGTCGCCTC CACACTGTGC ACACGCGGAA
    ACCACCTCAT CGTCTCATAC GTTGAATCCG GTGCCAGACG AGGCTCCGGT ACCGCAGCCG
    TACCGCGTTG ACGCTGTGCT CCTGCAGGAG CAGCCTCTCG AAGCACTTCC GCTGCCCGCG
    ATGCTCGACC CAGATGCTCA CTACGTCACG GCCATTGATG GAACGCCGAT CCCGCGAAGC
    ATGATTTCCG TGTCCGTGGA GCAGCTGGCT GTGAGCAAGG ACGATGAGCT TGCCCATTTC
    GCCACCAAGT CTGGCGCTGG CGCGTACGAA GAGCTAATTG TGGCTGACCA AGAGAAGAGT
    AATGCGGGGG CCGATCTCTT CATGGAGACG AGTGATGTAG CGCATCTTCC TGAGGAGGTG
    CAGACACAGT TGGAGGAGGC TTGTGCGCGT CTCACTCGCT CTGCAGCGCG CAAGCAGAAG
    GAGCGATTCC TTACAAAGTA TGTGGAGCGG AAGCGAAAGG AGCGATCGAT CTACATGCAG
    GCGGAGGGGT ACATGTGCCC GTGGCTAGGG AGGTCTGAGG GTCGGAGCGT GGTGCAGGGT
    TTCGCTGGCT CGTGCGGCAC CGGAAAAGGC GGGGCTGTCA TGCGTGACCT CAAGCAGAAG
    GGGTTCGGTG GTAAGAGCTA CTCTGCGCGG ACCATGAAGA ACAGAGGCCG CGCCACGAAA
    AAGACCGGGT TTTGGGCCGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001685628.1
    CDS1..2364
    Translation

    Target ORF information:

    RefSeq Version XM_001685576.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001685576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTCTTTTG CTACTTCTAT TACCTGTGTC CTTTTCTACA TGCTGCAACT CTCTCTGCTT 
    CTTCGTGGTG CTCTGTGCAT TTCGTATCGC GCACTGAAAA GCGCCGCAGC CGCCCCTGTC
    GCCGCAGCGG GGGTGTCAGC CGCTAGCTCG AGATTAGGCG GAGCATGGGA GGGTAGTATC
    GAGCGCGCTG AGGCTCTCGA GAACCTCAGC AACGAGGTGT TTGGCCGAGG AAACAGACAT
    TTGAAAAATG TGGAAACGCT GACGGCTCTC CCGGAAGGTC TGCACTCCTT CCCAGAAGTG
    TGCTTCATTG GGAAGCCAGG GTGCGGCAAG TCGAGCCTTA TCTCGTGTCT TTTGCATAAT
    CACCGGCTGG GCAAAGCCGG GGCAACTGCC GGCACTACGC GACTTCTGCA ATTTTTCAAT
    GTGGGCGACG CGCTTCTCTT GGTGGATACA CCAGGCTACG GTGGCTGGCG AGGTCGTGAG
    GTGGGACAGC GTCTAGCAGA GCAGGCCAAC GCGTTTTCGA TTCTGTTCCG CTACCTGGCC
    TTGCGCAACG GGAGTAATCT CAAGCGAGTA TACTGGCTCA TGGAGTCCTC GGCGCCGACG
    CCAGTGTCCT TCCAACCGCG TGACGAGGAG CTTCTAACTT TTCTCTCCCG GGAGCGCATT
    CCTTTTAGTG TCGTGCTCAC CAAGATCGAC CGCCACTGGC GCCACTACGC CGAGCAGCAG
    CGGACGGCTG ACCGAGTAGG AAACGATGGC TTCGTACACC CAAGGCACAG AAGACCAGAA
    ACACACGATC AGGGCTTTTT TACGCGCACG AGCCTGCCTC AAGACGGTGT GGCACGCAAT
    ATGCAGGAGG TGTATGAGTT TCTAGGCACC GATCAAGTAC CAATTCTCGG CGTAAGTGCC
    AACCGCCTAC AACCAAACCG TTCGCGCAAT CTGGAGCTGC TGCAGCACGA CATCGTTCAC
    TACTGCACGC AAGACCTGCT GCACTCAGAG GAGCTCTCTT TTCGCAACAT GCACAAGCTG
    AGCTACGCAC CACCGACGGC TGATCGTATT CATGAGATTC AGCTGCGATA CCCAGTGGAG
    TCGTTCGTTG TTCCCGAGAA CAACAACATG TCATTGGCAC GCATGGTGGA GCACCACGAG
    GAGATGAAAG CACGATTTTG CGAAAACAAC GTATGTGCGC GGCGCCTCTC CGCCAAAGAT
    GTGGCGGCGT GCCATCTAGA GAGCGTCGCA AAGCTGAAGG CGGCCTGTAA ACACGATACT
    GCAGAACGAG AGCGCGCGGA AAAGGTGCGT CCCACCGCGA CCTATCTCTT CGAATCTACC
    TTGGCTGCCG CTGTAACGTC GCTGCCGCTG CCAGGCGATG TCGCAGGCCT GTTGCGCGCC
    AGCGAAGCGC AGGGCACCGC TACTGCATCG GTAAGCCGTG TAGGGGGCCT TCCTGAGCGA
    TGGAAGGAAT GCGATGACCA CAATGCCGAG GCGTGCGCGG CTGACGTGTG CGCTAGCCGG
    CGCTTCCGCG TGCGGCCGCT CCTCGTAGGC GAGAGGCAGA CGAGCCGAGA CTCCTTGGGG
    GATATACTGG AGCGTGCGCT CGATGAGGAG GAGCCGAGGC AGGACTTTGC AGGTAGCATC
    GTGGACAAAG GCGAAAAGAC CGCCAACGCA GCGTCGCCTC CACACTGTGC ACACGCGGAA
    ACCACCTCAT CGTCTCATAC GTTGAATCCG GTGCCAGACG AGGCTCCGGT ACCGCAGCCG
    TACCGCGTTG ACGCTGTGCT CCTGCAGGAG CAGCCTCTCG AAGCACTTCC GCTGCCCGCG
    ATGCTCGACC CAGATGCTCA CTACGTCACG GCCATTGATG GAACGCCGAT CCCGCGAAGC
    ATGATTTCCG TGTCCGTGGA GCAGCTGGCT GTGAGCAAGG ACGATGAGCT TGCCCATTTC
    GCCACCAAGT CTGGCGCTGG CGCGTACGAA GAGCTAATTG TGGCTGACCA AGAGAAGAGT
    AATGCGGGGG CCGATCTCTT CATGGAGACG AGTGATGTAG CGCATCTTCC TGAGGAGGTG
    CAGACACAGT TGGAGGAGGC TTGTGCGCGT CTCACTCGCT CTGCAGCGCG CAAGCAGAAG
    GAGCGATTCC TTACAAAGTA TGTGGAGCGG AAGCGAAAGG AGCGATCGAT CTACATGCAG
    GCGGAGGGGT ACATGTGCCC GTGGCTAGGG AGGTCTGAGG GTCGGAGCGT GGTGCAGGGT
    TTCGCTGGCT CGTGCGGCAC CGGAAAAGGC GGGGCTGTCA TGCGTGACCT CAAGCAGAAG
    GGGTTCGGTG GTAAGAGCTA CTCTGCGCGG ACCATGAAGA ACAGAGGCCG CGCCACGAAA
    AAGACCGGGT TTTGGGCCGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ