LOC100209292 cDNA ORF clone, Hydra vulgaris

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100209292
Entrez Gene ID	100209292
Full Name	hemicentin-1-like
Gene Type	protein-coding
Organism	Hydra vulgaris

mRNA and Protein(s)

mRNA	Protein	Name
XM_012704455.1	XP_012559909.1	hemicentin-1-like

Pathways from BioSystems
Gene Ontology

The following LOC100209292 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100209292 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHc16715	XM_012704455.1 Latest version!	Hydra vulgaris hemicentin-1-like (LOC100209292), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100209292 ( XM_012704455.1 ) cDNA ORF clone, Hydra vulgaris -> XP_012559909.1 Hydra vulgaris hemicentin-1-like (LOC100209292), mRNA.

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CloneID	OHc16715
Clone ID Related Accession (Same CDS sequence)	XM_012704455.1
Accession Version	XM_012704455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433606400000
Organism	Hydra vulgaris
Product	hemicentin-1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004169555.1) and transcript sequence (GAOL01025689.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hydra vulgaris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1937 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTATACAT GCATGGTATT TAACAGATAT GAGAGACAAA AAAGTACTGC ATTTGTGAGG 
CTGATAGAGC CGTTTCAGCC AACAATATTT TCAACACGTC TTTCTCAGGA GCATTCAGAT 
GATGAAACTG AAGTTGCTAT AAATATTGGA ACTAATGCTA CATTTTATGA TGGAAGTAGT 
CTTAACATAA GATGTGAAGT TAAAGCTGCA GAGGAGTATT TTATAACTTG GATGTTTAAT 
TCTATTCCAT TAAATTCAAA TTCAGAAAGG TTGATTATTG AAAATACAAA ACAAGAAAAC 
AGTGGTTTGT ATTTTTGCAA GGTAAGCGAC AAAAGTGGGA AAGGAGATGA ACAGTTGTCT 
CATATCACTA TAATAAAAAA AATATTTCCA AAAATTGTTG AAGGAAACAA AGTATTAAGG 
TATATAAGAG GTAGAGGAGA TGCTCAAGTT ATGATTGGTG AAAATGCAAC TGTCAGAGAA 
GGTTTCACTG TTAAAATACA ATGTTCATAC TATGCTGTTC CTAAAGGAAA GTTAACTTGG 
GAAAGGCAAG GAGTCATCCT TTTAAATGAT TACCCAAATG AAAAGTCTCA CGATGATAAG 
TATGTTAGCC AATATATAAT ACGTACTGTT CAAATGAAAC ATTCTGGTGA ATATACTTGT 
GTAGCTGCCA ACAGAATGGG GAAGACTGCT GTTTCATCAA TAATTACTGT TATTGCTGCA 
AATTCTGGCC CACCAGTTAT TCAGAGTGGA TCTCGGAGAA GAACTGTAGA AATCTTATCT 
TCCAAAGCCT CAAATGTCGT TAAAGTTGGA GATTTTGTAA AAATTGTTGA GGGTGCACCT 
CTGAAGCTTT TGTGTAATGC TTCAGGTGTA CCACATCCAC ATATCACATG GTTTTTTGGT 
CGAGACCCTT TAGTAGATGA CTCAGTCATA CAAACATCTA CTATGCTTTT AATTTTAAAC 
TTTCAGCCTC ATCATGCTGG AGACTATAAA TGTGTTGCTA AAAATGCAGT TGGTGAAACT 
CGTGCATTTA CAGAAGTTCG GTTGATTGTT CCCAGTGCTC CAGTTATTAC TATTGGTCAT 
TCTGTTTTGA AAAATTCTGA CCCATTAAAA TCTAATGTTA TTACAATTGG AGATTATCTA 
AAAACACCTG GAGGTAGAAA TATTACTATA CGGTGTCCTG CTAAAGGCTA TCCAAATGTA 
GAACTTGTGT GGATGCATGA GAGTAAAATG TTACAAACCT CAGAAAAATT AATTGTTAAC 
TCAGCACAAA AAACACTAAC AATAATAAAT TCAGATGAGT GGGATACAGG GAAGTATGCA 
TGTTTTGCAA GTAACAGTGC TGGAATTTCA AGTGGATTTT CTTTTTTAAA AGTTTTAGCT 
CAAGGTTCAC CTATAATTAC TAGTTCTGAT GCAATAAAAA CTGATTTTGG AATTTCACCA 
TATGTTGTTA TCGAGATTGG TACAAATCTA ACTACTATTA CTGGGAAAAC ATTGAGTATT 
CTCTGTCCGT CTGAAGGTGC AGAGTCAACC TTTTATGAAT GGACAATAAA TAAAGAGAAT 
TTGAACTATG ATGCACGAAT AAATGTTTTG AATGGTAGTG AACTTATAAT TAATGATTTG 
CAACACTTTG ATGAAGGAAT TTATCAGTGT AGAGCTTTTA ATCAACATGG TTTTGATGCC 
ATGATTTCAA AAATTACAAT CATCAAAAAA CAGGATGAAA AACGACAACA AAATGACCCA 
GAAAATATTG AATCAGAAAA ACCTCAACAG AATGGCATTA AAAATATTGG TCCAGAAAAG 
CAACAACAAA ATGGCCCCTC AAATGTTGAT TCAGGTGTAA TTTTTAAAGA TGAAGTTACT 
GCTATTAAAG GATCAACTCT GGTTTTAACA TGTGCTTTAC CTGGTGCTAC AAGGCCACAG 
TTAAAATGGT ATAAAAATGG CCAATTTTTA TCTCACAGCA GCAAATATTT ACCTAGAGGT 
GAGCAGCTTG TTTTAAATAA TATTGATGAC AAATCTGCCG GGGAATACAC TTGCGAGTTA 
ACTGCAGATC TTGGTGTTAC ACTAATAATC TATAAAGTCA AGATTGAAGA TTTTCAACCT 
GTCACTATTA AAAAAAGCAA CAAAATCTAT AAAGTAACTG ACATTATGAA ACAAATAGAA 
GTTGTTATTG GAGACACAGT TAAATCAATT CAAGATGCAA ATATCAAACT TAAATGTCCT 
TATGTAGCTA GGCCAAATGC CACTGTTTAC TGGTTGCATA ACCAAAAAAT GATAAAGCAA 
GGAGATCTGT TTTTGATAAG TAATAATGCA AATGATCTGA TTATTCCAAA AATGGATAAA 
AGGTATGCTG GTTCTTACAC TTGTCGTGCT GTTCAACAGG ATAGGAAAGA TGAGGCCACA 
ACGCGAATTG AACTGCTTGA TTCAAAGCGC CCAATTATTT ATTCATCAAA TAAAAACTTG 
ACTTCAGAAG TTCTTTCTAG AGCAATTTTA AAAATAAATA TAGGTGATTC TCTTGTGGTA 
AACTTAAACA AACAAATTGA AATTACATGT TTGGGGGAAG GACTACCAAT TCCTAAAATT 
CTCTGGAAAT TTAATGGAGA AACAATAGAG CAATTAAACT TTGTGGATGA CCTTAAAAAT 
GGATCAATAT TAGTTAAAAA AGTTCTATGG GAGCATAATG GAAAATTTGA ATGTTTTTTT 
TTGAACTCTC TTGGATTTGA TTATGCAAGT AGCAGGATAT CAGTAGTAGG TGAGTTTACT 
CCTCATAACA TTAAACCCAC TGCACGCAAC GTGTTTAAAT TGAATACAAC TAAAGAAACA 
GTTTTGGTTG ATATTGGGAC AAATGTAACA ATAAGGACTC ATAATAATTT AAAAATGCTG 
TGCCCAGTTG ATAAATTTGG AAAAGTAGAT GTTGTCTGGT ATAAAAATGA TAAACAGTTG 
GAGTTATATG AAAGCACTAT ACAACTTAAT AATCTTGTAG TAGAGGATTC TGGGAGTTAT 
TTTTGTTTTA CAAATGATCC AGCAAAATTG AAGTTTGGTT CTGTTGAAGT TAATGTTTTA 
GCTCCATTCC ATGAACTTGT TGAGAGGTCT GATGTTCATC ATTTGGAGTA TCTAGAAAAT 
AAAATTAAAT TACTCAGTGC AGATGTAGGT GCACATGTTC TGATACTTTT GGGCAATTCA 
ATAAGATTCA GATGCCCTGT CAAGGAAAAC AGCAAAGTAA AAATTAGCTG GACAAAAAAT 
GGTGAAAATT TTGACGTGAA AGGTAACAAC TTTTACAGAG TTGTTGAATT TAAAGAGCAT 
GGCAATTTTA ATGTTTCATG TTTGATTACA AGCGTGTTAG GCTCTGATTT CTTTACTTCA 
CAAGTGACAG TTGTTGTACC AAAGTCTCCA ACAATTATCA AAGTTGAACA AAATACAACA 
TCTGTTAAAA CAAATTCTGT ATTTAGCATA GGTGGATCAA TAACAATTTT TGATGGAAGC 
AGTGTAGAGA TTAAATGTGA AACCATAGGT TTTCCAAAGC CAAAAATAGT TTGGAATTAT 
CAAGGCAGAG AGTTACAAAA TGCCAAAAAC GTTTATTTTA AGGAGGAAAG AGGAAAAACA 
TTAGCTTTAT TTTCTGTAAC AAAATCAAAT GAAGGCATTT ATTCATGCAC TGCATCAAAT 
GGTTTTGAAC CAGATGATAA ACAGAGTATT AAAGTTAATG TTAAAGTTCC AAGAAAACCA 
ACATTTGTTA AGAATAAAAA CCAAAATGAA ACAAAAATCT TGCAGATAGA AGGAAAATAT 
TCTTCAAGTT TGAAAAATAA AAATACACAG ATTAAAGATG TCGGAGAAAA CTTTGAAGTG 
TTAGCTGGCT CACCATTAAA ATTAAAATGT GATGTTGTAG GGGAACCGAA ACCTTCAATT 
GAATGGAGAA AAGATGGAGT GTATGTTTTT AAAGACAGCG AGCATTTTGT GATAGAGACA 
AATGATGTTC ATAATAGTGG TTTATATACA TGTACTGCCC TTAATGAATT TGGAGCTGTC 
AGTGAAAGTT CTATGATTAA CATTGTTGTT GCTAAAATAC CAATTTCTCA ACATTATAAT 
TTTGAAGTTA TTAAACACAC AAACGGATTG AAAAATGTGG ATGTTCTATT TGGAAACAAC 
TTGATGACTT TTACTGGAAC AGATGTTTCA CTGCGATGTC CAGCTAAAGG TGTTCCCGCA 
CCAAGTTTTA CTTTTTTTAA AGATGGAAAA CTTTTAATAA ATGAAAAAAA TATTACAGTT 
GATAATTCAG GGTTATTAAA GTTGTTCAAT GTTAATGAAA TGTCATCTGC AGGAGTTTAT 
ACATGTCGTG CTGAAAATAT TGCAGGGTTT ATAGAAATTA ACTCAACGCT AACACTCTAT 
TCTTCTAATA AGCCAAAGAT TAGTTCCATT GAAAAAGCAC CTTCGTATCA CTACAATAAA 
AATACAAAAG AGAGTGCCTT GTTCTTAACT ATTGGTCAAA ACACTACTAC ATATCCCAGC 
ACTAAAATAT ATATAGAGTG TCCTGTTGAG GGATTTCCAA CTCCAGATAT TGTTTGGAAA 
GTTAATGATG AAATTGTAAT GGAAGAAAGA GAAGCTAAAA TAAGTGACAA CAAAACAACT 
TTAATGTATG ATCTTGATCA TCAAGTTAGT CAGACAAGTT TTACTTGTAT AACAGTCAAT 
TTAGCAGGAT TTGATGAAAA AACATCACTT ATACAAGTTG TTGAAAAAAA GCAAGCAGGT 
ATTTTGTTGC CATATTTTAA ACCATTTAAT GATGAACATA TGGAGATCAA ATTAAACATT 
GGTGAAAGTG CAGACGTTTT GGTTGGATCA ACAGTTTCAA TATCATGTCC TGTAATTGGT 
GAGCCAATAC CTGAAGTAAT GTGGATAAAG CTAGGAAGAC CACTAGGTTG GTTACAATCC 
AACAGAAATG TTTCTATAAA AAACAGTCAC TTAACTTTAT ACAATGTAGA TCAAGATTTT 
AATGGGGAGT ACTTGTGCAA TGCAAGCAAT ATATTTGGTA CATCAATTAA AGCTTCAACT 
ATATTTATAA AAGAGAAAGA AGCACCAAAA ATACTTTCGT CAAATAAAAC ATTTGTTGCT 
GGTCATGGTG TAAATGTTAT TCGTGTTCTT ATTGGCACAA AAATCTATAC TAGAAAAACT 
TCACGAATAA TTATTGAATG CCCCGTATCA GGAACACCAC CTCCAAAGGT AATTTGGTAT 
AAAGGTCAAC TTGTTCTCAC TCATATATTT AAAGACACAA TCACTATTGA AAATGCAGAA 
AAGCATGATG GAGGCATTTA CTTTTGTGTT GCTACAAACA TAGCTGGGAG TGTACAAGTT 
AGCACAGAAC TAGTTGTTGG ATTCGAACCT CAAATGCAAA GTATTCCTCC TGATCTCTCT 
CCTGTTATCT CACTTGCGCA GAATTCTGAA GTATTTGTAC CTATAGGAGT AAATGAGGCA 
TTTGTTCGAA TGAATAACAC AGTACATTTA CAATGTTTTA CTGATGCTTT TCCTTCTCCA 
AGCATAGAAT GGTTTTATAA TGGTTTTGAA ATTGATTATG GTGATTTCAA ATTCAAAGCT 
GCAAATAACC ATGTTCTTAA AATTGCAAAA ATGACAACCA GAAATAGTGG TGTTTACTCT 
TGTATAGCAA AAAATCCGTT TGGACAAGCA AGTTCAAAAT CTAATTTGTA TTTAATGGAA 
CCGCCAATCA TTAAGGAAAA TAAAGAAATT TTGGTATTGA GAAAGATTCC AGATGAGGTC 
ATAAATATCC AAGTTGGAAG TCATCTCACT GTTTTATCTG GAACCTCATT TAGTTTATCT 
TGTTTTGTAA GTGGCTCACC TTTACCAACA ATACGATGGT TGAGAAATAA TATACGATTT 
GAGTTGAGCG AACGAATATC TTTTGCAACA CAAAAGTTAT ACTTCACAAA TGTTTTACCT 
CAAGACTCTG CTACGTATAC CTGTGAAGCT CGAAACAATT GGGGAAAAGT AAAGAGTTCA 
ACAATACTAC TTGTTGCAGA GCAACCTGTA GCGACTCTGT CTTTAAGTTT TTTGACCAAT 
TCTGGTGTAT CAACTGTAAG TGATGAATCT GTTGTTGTGC CAGCTGGATC TCATATAACT 
TTATTTTGTC ATGTGACTGG ATTTCCAATT CCTCGTATAG TTTGGTTAAA GGATCATGAT 
GTTTTTACTG GAACTGAAAA AAAATACCAA TTGTATAATC AATCAGCAAT TGAGGTAGAA 
CATTTTGAGC CTAAAGACAT GGGGTCCTAT ATATGTGTTG CAACAAATAT ATTAGGTGAA 
GCTACTGGGA TAATTAATTT ACTAATTGGA GTTTTGCCAA AGATCTCAAT TACTTTAGAC 
TCAATTGTTT CAAAAAACGA CTCAGTGATT GTACATGTTG GCTCTAATTT GGATGTGATG 
CCTGGTTCTA GTGTTAGAAT TATTTGTAAT GCTGCAGGAG AGCCAAAACC AAAAATATTA 
TGGGAAATAA CACCAAATAT AAACTCTGCT ATTCAGACAG TTCAGACAGA TAATGATGGG 
GAAATTGTGC TGAGGAATGT TTCTCATGAT GATGTGGGCT TATACACATG CGTTGCTCGA 
AATTCCATTG GTGTTGATAA AGCTGCATCT TCACTATCGA TAATGGAATC CCCAAAAATA 
CTATCTTTAA AGATTCAATA TTCGACTAAC ATTGGTGATG TTTTCTTTGC TAATGGAATG 
TTTCATGTTA TTGAAGGTGA ATCTATACTG CTAGTATGTG ATGTTGCTGG CTATCCATTA 
CCTATTATGA AGATGCAACA ATCAAACAGA TTAGGTTCAG TACATTTTAA TGAAAAGATT 
GGCAATGATT TTGAATCCAG TTTTCAAAAT TTTTCTTCAC ATCTTGAGTT GAATGTTAGT 
GAAACATTTG TTTTGCGCAA AAACAAAAAT GTTGTTGGTT TGCGTTCCCA ACTTTTTATT 
GGTAGCGCCA GACTAAATCA TTCATCATTT TACTTCTGTT ATGCAGAAAA TTATTTGGGT 
AGTGAGAAAG TAAATCTGGC TTTAGTTGTT CACAAAAAAC CTATAGATCC TTACAGGGGA 
GTATGGACAT CATCAAGTTT TAGTGAGTGT TCAAAACCTT GTGTTCGTTC AAACTATGGT 
ATTCAACAAC GTGAAATAAT ATGCTTCTCC TTTAAAGGTG AAGTAATGGA TCATGCTTAT 
TGTAATCCAC TTCTGAAAAT ATCATTACAA AGAGAATGTG AGAGTGCTAT ATGTGCTACT 
GAATGGAAAA CATCAGACTG GACAATGTGT TCTTCCACGT GTGGTGCGGA TGGTAAACAA 
TCAAGAGTTA CGTATTGTGG TTATAAAAAT TCTTATCTGA AAATATCTCC AAAAAATTGC 
ATTTTGCATG CAAAACCTGA AACAACTAGA GGTTGTAATA ATTTTCCATG TGATCAAGAG 
AGTTGCGTAG ACCGGAAGGT AGACTGCAGC ATCATAAAAG GATTAGGATT TTGTAAAATG 
GAAACATACA AAAGCCTTTG CTGCGAAAGT TGTCGAACAT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012559909.1
CDS	368..7912
Translation	MYTCMVFNRYERQKSTAFVRLIEPFQPTIFSTRLSQEHSDDETEVAINIGTNATFYDGSSLNIRCEVKAAEEYFITWMFNSIPLNSNSERLIIENTKQENSGLYFCKVSDKSGKGDEQLSHITIIKKIFPKIVEGNKVLRYIRGRGDAQVMIGENATVREGFTVKIQCSYYAVPKGKLTWERQGVILLNDYPNEKSHDDKYVSQYIIRTVQMKHSGEYTCVAANRMGKTAVSSIITVIAANSGPPVIQSGSRRRTVEILSSKASNVVKVGDFVKIVEGAPLKLLCNASGVPHPHITWFFGRDPLVDDSVIQTSTMLLILNFQPHHAGDYKCVAKNAVGETRAFTEVRLIVPSAPVITIGHSVLKNSDPLKSNVITIGDYLKTPGGRNITIRCPAKGYPNVELVWMHESKMLQTSEKLIVNSAQKTLTIINSDEWDTGKYACFASNSAGISSGFSFLKVLAQGSPIITSSDAIKTDFGISPYVVIEIGTNLTTITGKTLSILCPSEGAESTFYEWTINKENLNYDARINVLNGSELIINDLQHFDEGIYQCRAFNQHGFDAMISKITIIKKQDEKRQQNDPENIESEKPQQNGIKNIGPEKQQQNGPSNVDSGVIFKDEVTAIKGSTLVLTCALPGATRPQLKWYKNGQFLSHSSKYLPRGEQLVLNNIDDKSAGEYTCELTADLGVTLIIYKVKIEDFQPVTIKKSNKIYKVTDIMKQIEVVIGDTVKSIQDANIKLKCPYVARPNATVYWLHNQKMIKQGDLFLISNNANDLIIPKMDKRYAGSYTCRAVQQDRKDEATTRIELLDSKRPIIYSSNKNLTSEVLSRAILKINIGDSLVVNLNKQIEITCLGEGLPIPKILWKFNGETIEQLNFVDDLKNGSILVKKVLWEHNGKFECFFLNSLGFDYASSRISVVGEFTPHNIKPTARNVFKLNTTKETVLVDIGTNVTIRTHNNLKMLCPVDKFGKVDVVWYKNDKQLELYESTIQLNNLVVEDSGSYFCFTNDPAKLKFGSVEVNVLAPFHELVERSDVHHLEYLENKIKLLSADVGAHVLILLGNSIRFRCPVKENSKVKISWTKNGENFDVKGNNFYRVVEFKEHGNFNVSCLITSVLGSDFFTSQVTVVVPKSPTIIKVEQNTTSVKTNSVFSIGGSITIFDGSSVEIKCETIGFPKPKIVWNYQGRELQNAKNVYFKEERGKTLALFSVTKSNEGIYSCTASNGFEPDDKQSIKVNVKVPRKPTFVKNKNQNETKILQIEGKYSSSLKNKNTQIKDVGENFEVLAGSPLKLKCDVVGEPKPSIEWRKDGVYVFKDSEHFVIETNDVHNSGLYTCTALNEFGAVSESSMINIVVAKIPISQHYNFEVIKHTNGLKNVDVLFGNNLMTFTGTDVSLRCPAKGVPAPSFTFFKDGKLLINEKNITVDNSGLLKLFNVNEMSSAGVYTCRAENIAGFIEINSTLTLYSSNKPKISSIEKAPSYHYNKNTKESALFLTIGQNTTTYPSTKIYIECPVEGFPTPDIVWKVNDEIVMEEREAKISDNKTTLMYDLDHQVSQTSFTCITVNLAGFDEKTSLIQVVEKKQAGILLPYFKPFNDEHMEIKLNIGESADVLVGSTVSISCPVIGEPIPEVMWIKLGRPLGWLQSNRNVSIKNSHLTLYNVDQDFNGEYLCNASNIFGTSIKASTIFIKEKEAPKILSSNKTFVAGHGVNVIRVLIGTKIYTRKTSRIIIECPVSGTPPPKVIWYKGQLVLTHIFKDTITIENAEKHDGGIYFCVATNIAGSVQVSTELVVGFEPQMQSIPPDLSPVISLAQNSEVFVPIGVNEAFVRMNNTVHLQCFTDAFPSPSIEWFYNGFEIDYGDFKFKAANNHVLKIAKMTTRNSGVYSCIAKNPFGQASSKSNLYLMEPPIIKENKEILVLRKIPDEVINIQVGSHLTVLSGTSFSLSCFVSGSPLPTIRWLRNNIRFELSERISFATQKLYFTNVLPQDSATYTCEARNNWGKVKSSTILLVAEQPVATLSLSFLTNSGVSTVSDESVVVPAGSHITLFCHVTGFPIPRIVWLKDHDVFTGTEKKYQLYNQSAIEVEHFEPKDMGSYICVATNILGEATGIINLLIGVLPKISITLDSIVSKNDSVIVHVGSNLDVMPGSSVRIICNAAGEPKPKILWEITPNINSAIQTVQTDNDGEIVLRNVSHDDVGLYTCVARNSIGVDKAASSLSIMESPKILSLKIQYSTNIGDVFFANGMFHVIEGESILLVCDVAGYPLPIMKMQQSNRLGSVHFNEKIGNDFESSFQNFSSHLELNVSETFVLRKNKNVVGLRSQLFIGSARLNHSSFYFCYAENYLGSEKVNLALVVHKKPIDPYRGVWTSSSFSECSKPCVRSNYGIQQREIICFSFKGEVMDHAYCNPLLKISLQRECESAICATEWKTSDWTMCSSTCGADGKQSRVTYCGYKNSYLKISPKNCILHAKPETTRGCNNFPCDQESCVDRKVDCSIIKGLGFCKMETYKSLCCESCRTF

Target ORF information:

RefSeq Version	XM_012704455.1
Organism	Hydra vulgaris
Definition	Hydra vulgaris hemicentin-1-like (LOC100209292), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012704455.1

ORF Insert Sequence:

ATGTATACAT GCATGGTATT TAACAGATAT GAGAGACAAA AAAGTACTGC ATTTGTGAGG 
CTGATAGAGC CGTTTCAGCC AACAATATTT TCAACACGTC TTTCTCAGGA GCATTCAGAT 
GATGAAACTG AAGTTGCTAT AAATATTGGA ACTAATGCTA CATTTTATGA TGGAAGTAGT 
CTTAACATAA GATGTGAAGT TAAAGCTGCA GAGGAGTATT TTATAACTTG GATGTTTAAT 
TCTATTCCAT TAAATTCAAA TTCAGAAAGG TTGATTATTG AAAATACAAA ACAAGAAAAC 
AGTGGTTTGT ATTTTTGCAA GGTAAGCGAC AAAAGTGGGA AAGGAGATGA ACAGTTGTCT 
CATATCACTA TAATAAAAAA AATATTTCCA AAAATTGTTG AAGGAAACAA AGTATTAAGG 
TATATAAGAG GTAGAGGAGA TGCTCAAGTT ATGATTGGTG AAAATGCAAC TGTCAGAGAA 
GGTTTCACTG TTAAAATACA ATGTTCATAC TATGCTGTTC CTAAAGGAAA GTTAACTTGG 
GAAAGGCAAG GAGTCATCCT TTTAAATGAT TACCCAAATG AAAAGTCTCA CGATGATAAG 
TATGTTAGCC AATATATAAT ACGTACTGTT CAAATGAAAC ATTCTGGTGA ATATACTTGT 
GTAGCTGCCA ACAGAATGGG GAAGACTGCT GTTTCATCAA TAATTACTGT TATTGCTGCA 
AATTCTGGCC CACCAGTTAT TCAGAGTGGA TCTCGGAGAA GAACTGTAGA AATCTTATCT 
TCCAAAGCCT CAAATGTCGT TAAAGTTGGA GATTTTGTAA AAATTGTTGA GGGTGCACCT 
CTGAAGCTTT TGTGTAATGC TTCAGGTGTA CCACATCCAC ATATCACATG GTTTTTTGGT 
CGAGACCCTT TAGTAGATGA CTCAGTCATA CAAACATCTA CTATGCTTTT AATTTTAAAC 
TTTCAGCCTC ATCATGCTGG AGACTATAAA TGTGTTGCTA AAAATGCAGT TGGTGAAACT 
CGTGCATTTA CAGAAGTTCG GTTGATTGTT CCCAGTGCTC CAGTTATTAC TATTGGTCAT 
TCTGTTTTGA AAAATTCTGA CCCATTAAAA TCTAATGTTA TTACAATTGG AGATTATCTA 
AAAACACCTG GAGGTAGAAA TATTACTATA CGGTGTCCTG CTAAAGGCTA TCCAAATGTA 
GAACTTGTGT GGATGCATGA GAGTAAAATG TTACAAACCT CAGAAAAATT AATTGTTAAC 
TCAGCACAAA AAACACTAAC AATAATAAAT TCAGATGAGT GGGATACAGG GAAGTATGCA 
TGTTTTGCAA GTAACAGTGC TGGAATTTCA AGTGGATTTT CTTTTTTAAA AGTTTTAGCT 
CAAGGTTCAC CTATAATTAC TAGTTCTGAT GCAATAAAAA CTGATTTTGG AATTTCACCA 
TATGTTGTTA TCGAGATTGG TACAAATCTA ACTACTATTA CTGGGAAAAC ATTGAGTATT 
CTCTGTCCGT CTGAAGGTGC AGAGTCAACC TTTTATGAAT GGACAATAAA TAAAGAGAAT 
TTGAACTATG ATGCACGAAT AAATGTTTTG AATGGTAGTG AACTTATAAT TAATGATTTG 
CAACACTTTG ATGAAGGAAT TTATCAGTGT AGAGCTTTTA ATCAACATGG TTTTGATGCC 
ATGATTTCAA AAATTACAAT CATCAAAAAA CAGGATGAAA AACGACAACA AAATGACCCA 
GAAAATATTG AATCAGAAAA ACCTCAACAG AATGGCATTA AAAATATTGG TCCAGAAAAG 
CAACAACAAA ATGGCCCCTC AAATGTTGAT TCAGGTGTAA TTTTTAAAGA TGAAGTTACT 
GCTATTAAAG GATCAACTCT GGTTTTAACA TGTGCTTTAC CTGGTGCTAC AAGGCCACAG 
TTAAAATGGT ATAAAAATGG CCAATTTTTA TCTCACAGCA GCAAATATTT ACCTAGAGGT 
GAGCAGCTTG TTTTAAATAA TATTGATGAC AAATCTGCCG GGGAATACAC TTGCGAGTTA 
ACTGCAGATC TTGGTGTTAC ACTAATAATC TATAAAGTCA AGATTGAAGA TTTTCAACCT 
GTCACTATTA AAAAAAGCAA CAAAATCTAT AAAGTAACTG ACATTATGAA ACAAATAGAA 
GTTGTTATTG GAGACACAGT TAAATCAATT CAAGATGCAA ATATCAAACT TAAATGTCCT 
TATGTAGCTA GGCCAAATGC CACTGTTTAC TGGTTGCATA ACCAAAAAAT GATAAAGCAA 
GGAGATCTGT TTTTGATAAG TAATAATGCA AATGATCTGA TTATTCCAAA AATGGATAAA 
AGGTATGCTG GTTCTTACAC TTGTCGTGCT GTTCAACAGG ATAGGAAAGA TGAGGCCACA 
ACGCGAATTG AACTGCTTGA TTCAAAGCGC CCAATTATTT ATTCATCAAA TAAAAACTTG 
ACTTCAGAAG TTCTTTCTAG AGCAATTTTA AAAATAAATA TAGGTGATTC TCTTGTGGTA 
AACTTAAACA AACAAATTGA AATTACATGT TTGGGGGAAG GACTACCAAT TCCTAAAATT 
CTCTGGAAAT TTAATGGAGA AACAATAGAG CAATTAAACT TTGTGGATGA CCTTAAAAAT 
GGATCAATAT TAGTTAAAAA AGTTCTATGG GAGCATAATG GAAAATTTGA ATGTTTTTTT 
TTGAACTCTC TTGGATTTGA TTATGCAAGT AGCAGGATAT CAGTAGTAGG TGAGTTTACT 
CCTCATAACA TTAAACCCAC TGCACGCAAC GTGTTTAAAT TGAATACAAC TAAAGAAACA 
GTTTTGGTTG ATATTGGGAC AAATGTAACA ATAAGGACTC ATAATAATTT AAAAATGCTG 
TGCCCAGTTG ATAAATTTGG AAAAGTAGAT GTTGTCTGGT ATAAAAATGA TAAACAGTTG 
GAGTTATATG AAAGCACTAT ACAACTTAAT AATCTTGTAG TAGAGGATTC TGGGAGTTAT 
TTTTGTTTTA CAAATGATCC AGCAAAATTG AAGTTTGGTT CTGTTGAAGT TAATGTTTTA 
GCTCCATTCC ATGAACTTGT TGAGAGGTCT GATGTTCATC ATTTGGAGTA TCTAGAAAAT 
AAAATTAAAT TACTCAGTGC AGATGTAGGT GCACATGTTC TGATACTTTT GGGCAATTCA 
ATAAGATTCA GATGCCCTGT CAAGGAAAAC AGCAAAGTAA AAATTAGCTG GACAAAAAAT 
GGTGAAAATT TTGACGTGAA AGGTAACAAC TTTTACAGAG TTGTTGAATT TAAAGAGCAT 
GGCAATTTTA ATGTTTCATG TTTGATTACA AGCGTGTTAG GCTCTGATTT CTTTACTTCA 
CAAGTGACAG TTGTTGTACC AAAGTCTCCA ACAATTATCA AAGTTGAACA AAATACAACA 
TCTGTTAAAA CAAATTCTGT ATTTAGCATA GGTGGATCAA TAACAATTTT TGATGGAAGC 
AGTGTAGAGA TTAAATGTGA AACCATAGGT TTTCCAAAGC CAAAAATAGT TTGGAATTAT 
CAAGGCAGAG AGTTACAAAA TGCCAAAAAC GTTTATTTTA AGGAGGAAAG AGGAAAAACA 
TTAGCTTTAT TTTCTGTAAC AAAATCAAAT GAAGGCATTT ATTCATGCAC TGCATCAAAT 
GGTTTTGAAC CAGATGATAA ACAGAGTATT AAAGTTAATG TTAAAGTTCC AAGAAAACCA 
ACATTTGTTA AGAATAAAAA CCAAAATGAA ACAAAAATCT TGCAGATAGA AGGAAAATAT 
TCTTCAAGTT TGAAAAATAA AAATACACAG ATTAAAGATG TCGGAGAAAA CTTTGAAGTG 
TTAGCTGGCT CACCATTAAA ATTAAAATGT GATGTTGTAG GGGAACCGAA ACCTTCAATT 
GAATGGAGAA AAGATGGAGT GTATGTTTTT AAAGACAGCG AGCATTTTGT GATAGAGACA 
AATGATGTTC ATAATAGTGG TTTATATACA TGTACTGCCC TTAATGAATT TGGAGCTGTC 
AGTGAAAGTT CTATGATTAA CATTGTTGTT GCTAAAATAC CAATTTCTCA ACATTATAAT 
TTTGAAGTTA TTAAACACAC AAACGGATTG AAAAATGTGG ATGTTCTATT TGGAAACAAC 
TTGATGACTT TTACTGGAAC AGATGTTTCA CTGCGATGTC CAGCTAAAGG TGTTCCCGCA 
CCAAGTTTTA CTTTTTTTAA AGATGGAAAA CTTTTAATAA ATGAAAAAAA TATTACAGTT 
GATAATTCAG GGTTATTAAA GTTGTTCAAT GTTAATGAAA TGTCATCTGC AGGAGTTTAT 
ACATGTCGTG CTGAAAATAT TGCAGGGTTT ATAGAAATTA ACTCAACGCT AACACTCTAT 
TCTTCTAATA AGCCAAAGAT TAGTTCCATT GAAAAAGCAC CTTCGTATCA CTACAATAAA 
AATACAAAAG AGAGTGCCTT GTTCTTAACT ATTGGTCAAA ACACTACTAC ATATCCCAGC 
ACTAAAATAT ATATAGAGTG TCCTGTTGAG GGATTTCCAA CTCCAGATAT TGTTTGGAAA 
GTTAATGATG AAATTGTAAT GGAAGAAAGA GAAGCTAAAA TAAGTGACAA CAAAACAACT 
TTAATGTATG ATCTTGATCA TCAAGTTAGT CAGACAAGTT TTACTTGTAT AACAGTCAAT 
TTAGCAGGAT TTGATGAAAA AACATCACTT ATACAAGTTG TTGAAAAAAA GCAAGCAGGT 
ATTTTGTTGC CATATTTTAA ACCATTTAAT GATGAACATA TGGAGATCAA ATTAAACATT 
GGTGAAAGTG CAGACGTTTT GGTTGGATCA ACAGTTTCAA TATCATGTCC TGTAATTGGT 
GAGCCAATAC CTGAAGTAAT GTGGATAAAG CTAGGAAGAC CACTAGGTTG GTTACAATCC 
AACAGAAATG TTTCTATAAA AAACAGTCAC TTAACTTTAT ACAATGTAGA TCAAGATTTT 
AATGGGGAGT ACTTGTGCAA TGCAAGCAAT ATATTTGGTA CATCAATTAA AGCTTCAACT 
ATATTTATAA AAGAGAAAGA AGCACCAAAA ATACTTTCGT CAAATAAAAC ATTTGTTGCT 
GGTCATGGTG TAAATGTTAT TCGTGTTCTT ATTGGCACAA AAATCTATAC TAGAAAAACT 
TCACGAATAA TTATTGAATG CCCCGTATCA GGAACACCAC CTCCAAAGGT AATTTGGTAT 
AAAGGTCAAC TTGTTCTCAC TCATATATTT AAAGACACAA TCACTATTGA AAATGCAGAA 
AAGCATGATG GAGGCATTTA CTTTTGTGTT GCTACAAACA TAGCTGGGAG TGTACAAGTT 
AGCACAGAAC TAGTTGTTGG ATTCGAACCT CAAATGCAAA GTATTCCTCC TGATCTCTCT 
CCTGTTATCT CACTTGCGCA GAATTCTGAA GTATTTGTAC CTATAGGAGT AAATGAGGCA 
TTTGTTCGAA TGAATAACAC AGTACATTTA CAATGTTTTA CTGATGCTTT TCCTTCTCCA 
AGCATAGAAT GGTTTTATAA TGGTTTTGAA ATTGATTATG GTGATTTCAA ATTCAAAGCT 
GCAAATAACC ATGTTCTTAA AATTGCAAAA ATGACAACCA GAAATAGTGG TGTTTACTCT 
TGTATAGCAA AAAATCCGTT TGGACAAGCA AGTTCAAAAT CTAATTTGTA TTTAATGGAA 
CCGCCAATCA TTAAGGAAAA TAAAGAAATT TTGGTATTGA GAAAGATTCC AGATGAGGTC 
ATAAATATCC AAGTTGGAAG TCATCTCACT GTTTTATCTG GAACCTCATT TAGTTTATCT 
TGTTTTGTAA GTGGCTCACC TTTACCAACA ATACGATGGT TGAGAAATAA TATACGATTT 
GAGTTGAGCG AACGAATATC TTTTGCAACA CAAAAGTTAT ACTTCACAAA TGTTTTACCT 
CAAGACTCTG CTACGTATAC CTGTGAAGCT CGAAACAATT GGGGAAAAGT AAAGAGTTCA 
ACAATACTAC TTGTTGCAGA GCAACCTGTA GCGACTCTGT CTTTAAGTTT TTTGACCAAT 
TCTGGTGTAT CAACTGTAAG TGATGAATCT GTTGTTGTGC CAGCTGGATC TCATATAACT 
TTATTTTGTC ATGTGACTGG ATTTCCAATT CCTCGTATAG TTTGGTTAAA GGATCATGAT 
GTTTTTACTG GAACTGAAAA AAAATACCAA TTGTATAATC AATCAGCAAT TGAGGTAGAA 
CATTTTGAGC CTAAAGACAT GGGGTCCTAT ATATGTGTTG CAACAAATAT ATTAGGTGAA 
GCTACTGGGA TAATTAATTT ACTAATTGGA GTTTTGCCAA AGATCTCAAT TACTTTAGAC 
TCAATTGTTT CAAAAAACGA CTCAGTGATT GTACATGTTG GCTCTAATTT GGATGTGATG 
CCTGGTTCTA GTGTTAGAAT TATTTGTAAT GCTGCAGGAG AGCCAAAACC AAAAATATTA 
TGGGAAATAA CACCAAATAT AAACTCTGCT ATTCAGACAG TTCAGACAGA TAATGATGGG 
GAAATTGTGC TGAGGAATGT TTCTCATGAT GATGTGGGCT TATACACATG CGTTGCTCGA 
AATTCCATTG GTGTTGATAA AGCTGCATCT TCACTATCGA TAATGGAATC CCCAAAAATA 
CTATCTTTAA AGATTCAATA TTCGACTAAC ATTGGTGATG TTTTCTTTGC TAATGGAATG 
TTTCATGTTA TTGAAGGTGA ATCTATACTG CTAGTATGTG ATGTTGCTGG CTATCCATTA 
CCTATTATGA AGATGCAACA ATCAAACAGA TTAGGTTCAG TACATTTTAA TGAAAAGATT 
GGCAATGATT TTGAATCCAG TTTTCAAAAT TTTTCTTCAC ATCTTGAGTT GAATGTTAGT 
GAAACATTTG TTTTGCGCAA AAACAAAAAT GTTGTTGGTT TGCGTTCCCA ACTTTTTATT 
GGTAGCGCCA GACTAAATCA TTCATCATTT TACTTCTGTT ATGCAGAAAA TTATTTGGGT 
AGTGAGAAAG TAAATCTGGC TTTAGTTGTT CACAAAAAAC CTATAGATCC TTACAGGGGA 
GTATGGACAT CATCAAGTTT TAGTGAGTGT TCAAAACCTT GTGTTCGTTC AAACTATGGT 
ATTCAACAAC GTGAAATAAT ATGCTTCTCC TTTAAAGGTG AAGTAATGGA TCATGCTTAT 
TGTAATCCAC TTCTGAAAAT ATCATTACAA AGAGAATGTG AGAGTGCTAT ATGTGCTACT 
GAATGGAAAA CATCAGACTG GACAATGTGT TCTTCCACGT GTGGTGCGGA TGGTAAACAA 
TCAAGAGTTA CGTATTGTGG TTATAAAAAT TCTTATCTGA AAATATCTCC AAAAAATTGC 
ATTTTGCATG CAAAACCTGA AACAACTAGA GGTTGTAATA ATTTTCCATG TGATCAAGAG 
AGTTGCGTAG ACCGGAAGGT AGACTGCAGC ATCATAAAAG GATTAGGATT TTGTAAAATG 
GAAACATACA AAAGCCTTTG CTGCGAAAGT TGTCGAACAT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100209292 cDNA ORF clone, Hydra vulgaris

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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