GCC2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCC2
Entrez Gene ID	9648
Full Name	GRIP and coiled-coil domain containing 2
Synonyms	GCC185,RANBP2L4,REN53
General protein information	Preferred Names GRIP and coiled-coil domain containing 2 Names GRIP and coiled-coil domain-containing protein 2 185 kDa Golgi coiled-coil protein CLL-associated antigen KW-11 CTCL tumor antigen se1-1 GCC protein, 185-kD Golgi coiled-coil protein GCC185 Ran-binding protein 2-like 4 renal carcinoma antigen NY-REN-53
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q12.3
Summary	The protein encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009].
Disorder MIM:	612711

mRNA and Protein(s)

mRNA	Protein	Name
XM_011512213.2	XP_011510515.1	GRIP and coiled-coil domain-containing protein 2 isoform X3
NM_181453.3	NP_852118.1	GRIP and coiled-coil domain-containing protein 2
NM_181453.4	NP_852118.2	GRIP and coiled-coil domain-containing protein 2
XM_006712871.1	XP_006712934.1	GRIP and coiled-coil domain-containing protein 2 isoform X2
XM_006712870.3	XP_006712933.1	GRIP and coiled-coil domain-containing protein 2 isoform X1
XM_006712872.3	XP_006712935.1	GRIP and coiled-coil domain-containing protein 2 isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	GCC2	NP_852118.1
Bos taurus (cattle)	GCC2	NP_001179188.1
Rattus norvegicus (Norway rat)	Gcc2	NP_001101103.1
Macaca mulatta (Rhesus monkey)	LOC693729	XP_002808095.1
Gallus gallus (chicken)	GCC2	XP_425585.3
Pan troglodytes (chimpanzee)	GCC2	XP_001135112.2
Xenopus tropicalis (tropical clawed frog)	gcc2	XP_002937710.2
Mus musculus (house mouse)	Gcc2	NP_081651.2
Canis lupus familiaris (dog)	GCC2	XP_531770.3

Gene Ontology
Bibliography

Related articles in PubMed

HIV-1 Nef binds with human GCC185 protein and regulates mannose 6 phosphate receptor recycling.
Kumar M, Kaur S, Nazir A, Tripathi RK
Biochemical and biophysical research communications474(1)137-145(2016 05)

Protein flexibility is required for vesicle tethering at the Golgi.
Cheung PY, Limouse C, Mabuchi H, Pfeffer SR
eLife4(2015 Dec)

ARL4A acts with GCC185 to modulate Golgi complex organization.
Lin YC, Chiang TC, Liu YT, Tsai YT, Jang LT, Lee FJ
Journal of cell science124(Pt 23)4014-26(2011 Dec)

GCC185 plays independent roles in Golgi structure maintenance and AP-1-mediated vesicle tethering.
Brown FC, Schindelhaim CH, Pfeffer SR
The Journal of cell biology194(5)779-87(2011 Sep)

Multiple Rab GTPase binding sites in GCC185 suggest a model for vesicle tethering at the trans-Golgi.
Hayes GL, Brown FC, Haas AK, Nottingham RM, Barr FA, Pfeffer SR
Molecular biology of the cell20(1)209-17(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GCC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu36855	XM_006712872.3 Latest version!	Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu108354	NM_181453.4 Latest version!	Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu31795	NM_181453.3 Latest version!	Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu36853	XM_006712870.3 Latest version!	Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu36854	XM_006712871.1 Latest version!	Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu63067	XM_011512213.2 Latest version!	Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCC2 ( XM_006712872.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712935.1 Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X8, mRNA.

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CloneID	OHu36855
Clone ID Related Accession (Same CDS sequence)	XM_006712872.3
Accession Version	XM_006712872.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	GRIP and coiled-coil domain-containing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712872.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTAA CTCAGGATCT TGTTCAAGAT GGGGTGGCTT CACCAGCTAC CCCTGGGACC 
GGGAAATCTA AGCTGGAAAC ATTGCCCAAA GAAGACCTCA TCAAGTTTGC CAAGAAACAG 
ATGATGCTAA TACAGAAAGC TAAATCAAGG TGTACAGAAT TGGAGAAAGA AATTGAAGAA 
CTCAGATCAA AACCTGTTAC TGAAGGAACT GGTGATATTA TTAAGGCATT AACTGAACGT 
CTGGATGCTC TTCTTCTGGA AAAAGCAGAG ACTGAGCAAC AGTGTCTTTC TCTGAAAAAG 
GAAAATATAA AAATGAAGCA AGAGGTTGAG GATTCTGTAA CAAAGATGGG AGATGCACAT 
AAGGAGTTGG AACAATCACA TATAAACTAT GTGAAAGAAA TTGAAAATTT GAAAAATGAG 
TTGATGGCAG TACGTTCCAA ATACAGTGAA GACAAAGCTA ACTTACAAAA GCAGCTGGAA 
GAAGCAATGA ATACGCAATT AGAACTTTCA GAACAACTTA AATTTCAGAA CAACTCTGAA 
GATAATGTTA AAAAACTACA AGAAGAGATT GAGAAAATTA GGCCAGGCTT TGAGGAGCAA 
ATTTTATATC TGCAAAAGCA ATTAGACGCT ACCACTGATG AAAAGAAGGA AACAGTTACT 
CAACTCCAAA ATATCATTGA GGCTAATTCT CAGCATTACC AAAAAAATAT TAATAGTTTG 
CAGGAAGAGC TTTTACAGTT GAAAGCTATA CACCAAGAAG AGGTGAAAGA GTTGATGTGC 
CAGATTGAAG CATCAGCTAA GGAACATGAA GCAGAGATAA ATAAGTTGAA CGAGCTAAAA 
GAGAACTTAG TAAAACAATG TGAGGCAAGT GAAAAGAACA TCCAGAAGAA ATATGAATGT 
GAGTTAGAAA ATTTAAGGAA AGCCACCTCA AATGCAAACC AAGACAATCA GATATGTTCT 
ATTCTCTTGC AAGAAAATAC ATTTGTAGAA CAAGTAGTAA ATGAAAAAGT CAAACACTTA 
GAAGATACCT TAAAAGAACT TGAATCTCAA CACAGTATCT TAAAAGATGA GGTAACTTAT 
ATGAATAATC TTAAGTTAAA ACTTGAAATG GATGCTCAAC ATATAAAGGA TGAGTTTTTT 
CATGAACGGG AAGACTTAGA GTTTAAAATT AATGAATTAT TACTAGCTAA AGAAGAACAG 
GGCTGTGTAA TTGAAAAATT AAAATCTGAG CTAGCAGGTT TAAATAAACA GTTTTGCTAT 
ACTGTAGAAC AGCATAACAG AGAAGTACAG AGTCTTAAGG AACAACATCA AAAAGAAATA 
TCAGAACTAA ATGAGACATT TTTGTCAGAT TCAGAAAAAG AAAAATTAAC ATTAATGTTT 
GAAATACAGG GTCTTAAGGA ACAGTGTGAA AACCTACAGC AAGAAAAGCA AGAAGCAATT 
TTAAATTATG AGAGTTTACG AGAGATTATG GAAATTTTAC AAACAGAACT GGGGGAATCT 
GCTGGAAAAA TAAGTCAAGA GTTCGAATCA ATGAAGCAAC AGCAAGCATC TGATGTTCAT 
GAACTGCAGC AGAAGCTCAG AACTGCTTTT ACTGAAAAAG ATGCCCTTCT CGAAACTGTG 
AATCGCCTCC AGGGAGAAAA TGAAAAGTTA CTATCTCAAC AAGAATTGGT ACCAGAACTT 
GAAAATACCA TAAAGAACCT TCAAGAAAAG AATGGAGTAT ACTTACTTAG TCTCAGTCAA 
AGAGATACCA TGTTAAAAGA ATTAGAAGGA AAGATAAATT CTCTTACTGA GGAAAAAGAT 
GATTTTATAA ATAAACTGAA AAATTCCCAT GAAGAAATGG ATAATTTCCA TAAGAAATGT 
GAAAGGGAAG AAAGATTGAT TCTTGAACTT GGGAAGAAAG TAGAGCAAAC AATCCAGTAC 
AACAGTGAAC TAGAACAAAA GGTAAATGAA TTAACAGGAG GACTAGAGGA GACTTTAAAA 
GAAAAGGATC AAAATGACCA AAAACTAGAA AAACTTATGG TTCAAATGAA AGTTCTCTCT 
GAAGACAAAG AAGTATTGTC AGCTGAAGTG AAGTCTCTTT ATGAGGAAAA CAATAAACTC 
AGTTCAGAAA AAAAACAGTT GAGTAGGGAT TTGGAGGTTT TTTTGTCTCA AAAAGAAGAT 
GTTATCCTTA AAGAACATAT TACTCAATTA GAAAAGAAAC TTCAGTTAAT GGTTGAAGAG 
CAAGATAATT TAAATAAACT GCTTGAAAAT GAGCAAGTTC AGAAGTTATT TGTTAAAACT 
CAGTTGTATG GTTTTCTTAA AGAAATGGGA TCAGAAGTTT CAGAAGACAG TGAAGAGAAA 
GATGTTGTTA ATGTCCTACA GGCAGTCGGT GAATCCTTGG CAAAAATAAA TGAGGAAAAA 
TGCAACCTGG CTTTTCAGCG TGATGAAAAA GTATTAGAGT TAGAAAAAGA GATTAAGTGC 
CTTCAAGAAG AGAGTGTAGT TCAGTGTGAA GAACTTAAGT CTTTATTGAG AGACTATGAG 
CAAGAGAAAG TTCTCTTAAG GAAAGAGTTA GAAGAAATAC AGTCAGAAAA AGAGGCCCTG 
CAGTCTGATC TTCTAGAAAT GAAGAATGCT AATGAAAAAA CAAGGCTTGA AAATCAGAAT 
CTTTTAATTC AAGTTGAAGA AGTATCTCAA ACATGTAGCA AAAGTGAAAT CCATAATGAA 
AAAGAAAAAT GTTTTATAAA GGAACATGAA AACCTAAAGC CACTACTAGA ACAAAAAGAA 
TTACGAGATA GGAGAGCAGA GTTGATACTA TTAAAGGATT CCTTAGCAAA ATCACCTTCT 
GTAAAAAATG ATCCTCTGTC TTCAGTAAAA GAGTTGGAAG AAAAAATAGG AAAAAATCGA 
CTAGAAAGGG GAAAATTATT GCTGCAGAAG AAGGAGGGAG AATTGTTGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712935.1
CDS	715..3648
Translation	MEVTQDLVQDGVASPATPGTGKSKLETLPKEDLIKFAKKQMMLIQKAKSRCTELEKEIEELRSKPVTEGTGDIIKALTERLDALLLEKAETEQQCLSLKKENIKMKQEVEDSVTKMGDAHKELEQSHINYVKEIENLKNELMAVRSKYSEDKANLQKQLEEAMNTQLELSEQLKFQNNSEDNVKKLQEEIEKIRPGFEEQILYLQKQLDATTDEKKETVTQLQNIIEANSQHYQKNINSLQEELLQLKAIHQEEVKELMCQIEASAKEHEAEINKLNELKENLVKQCEASEKNIQKKYECELENLRKATSNANQDNQICSILLQENTFVEQVVNEKVKHLEDTLKELESQHSILKDEVTYMNNLKLKLEMDAQHIKDEFFHEREDLEFKINELLLAKEEQGCVIEKLKSELAGLNKQFCYTVEQHNREVQSLKEQHQKEISELNETFLSDSEKEKLTLMFEIQGLKEQCENLQQEKQEAILNYESLREIMEILQTELGESAGKISQEFESMKQQQASDVHELQQKLRTAFTEKDALLETVNRLQGENEKLLSQQELVPELENTIKNLQEKNGVYLLSLSQRDTMLKELEGKINSLTEEKDDFINKLKNSHEEMDNFHKKCEREERLILELGKKVEQTIQYNSELEQKVNELTGGLEETLKEKDQNDQKLEKLMVQMKVLSEDKEVLSAEVKSLYEENNKLSSEKKQLSRDLEVFLSQKEDVILKEHITQLEKKLQLMVEEQDNLNKLLENEQVQKLFVKTQLYGFLKEMGSEVSEDSEEKDVVNVLQAVGESLAKINEEKCNLAFQRDEKVLELEKEIKCLQEESVVQCEELKSLLRDYEQEKVLLRKELEEIQSEKEALQSDLLEMKNANEKTRLENQNLLIQVEEVSQTCSKSEIHNEKEKCFIKEHENLKPLLEQKELRDRRAELILLKDSLAKSPSVKNDPLSSVKELEEKIGKNRLERGKLLLQKKEGELLL

Target ORF information:

RefSeq Version	XM_006712872.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712872.3

ORF Insert Sequence:

ATGGAGGTAA CTCAGGATCT TGTTCAAGAT GGGGTGGCTT CACCAGCTAC CCCTGGGACC 
GGGAAATCTA AGCTGGAAAC ATTGCCCAAA GAAGACCTCA TCAAGTTTGC CAAGAAACAG 
ATGATGCTAA TACAGAAAGC TAAATCAAGG TGTACAGAAT TGGAGAAAGA AATTGAAGAA 
CTCAGATCAA AACCTGTTAC TGAAGGAACT GGTGATATTA TTAAGGCATT AACTGAACGT 
CTGGATGCTC TTCTTCTGGA AAAAGCAGAG ACTGAGCAAC AGTGTCTTTC TCTGAAAAAG 
GAAAATATAA AAATGAAGCA AGAGGTTGAG GATTCTGTAA CAAAGATGGG AGATGCACAT 
AAGGAGTTGG AACAATCACA TATAAACTAT GTGAAAGAAA TTGAAAATTT GAAAAATGAG 
TTGATGGCAG TACGTTCCAA ATACAGTGAA GACAAAGCTA ACTTACAAAA GCAGCTGGAA 
GAAGCAATGA ATACGCAATT AGAACTTTCA GAACAACTTA AATTTCAGAA CAACTCTGAA 
GATAATGTTA AAAAACTACA AGAAGAGATT GAGAAAATTA GGCCAGGCTT TGAGGAGCAA 
ATTTTATATC TGCAAAAGCA ATTAGACGCT ACCACTGATG AAAAGAAGGA AACAGTTACT 
CAACTCCAAA ATATCATTGA GGCTAATTCT CAGCATTACC AAAAAAATAT TAATAGTTTG 
CAGGAAGAGC TTTTACAGTT GAAAGCTATA CACCAAGAAG AGGTGAAAGA GTTGATGTGC 
CAGATTGAAG CATCAGCTAA GGAACATGAA GCAGAGATAA ATAAGTTGAA CGAGCTAAAA 
GAGAACTTAG TAAAACAATG TGAGGCAAGT GAAAAGAACA TCCAGAAGAA ATATGAATGT 
GAGTTAGAAA ATTTAAGGAA AGCCACCTCA AATGCAAACC AAGACAATCA GATATGTTCT 
ATTCTCTTGC AAGAAAATAC ATTTGTAGAA CAAGTAGTAA ATGAAAAAGT CAAACACTTA 
GAAGATACCT TAAAAGAACT TGAATCTCAA CACAGTATCT TAAAAGATGA GGTAACTTAT 
ATGAATAATC TTAAGTTAAA ACTTGAAATG GATGCTCAAC ATATAAAGGA TGAGTTTTTT 
CATGAACGGG AAGACTTAGA GTTTAAAATT AATGAATTAT TACTAGCTAA AGAAGAACAG 
GGCTGTGTAA TTGAAAAATT AAAATCTGAG CTAGCAGGTT TAAATAAACA GTTTTGCTAT 
ACTGTAGAAC AGCATAACAG AGAAGTACAG AGTCTTAAGG AACAACATCA AAAAGAAATA 
TCAGAACTAA ATGAGACATT TTTGTCAGAT TCAGAAAAAG AAAAATTAAC ATTAATGTTT 
GAAATACAGG GTCTTAAGGA ACAGTGTGAA AACCTACAGC AAGAAAAGCA AGAAGCAATT 
TTAAATTATG AGAGTTTACG AGAGATTATG GAAATTTTAC AAACAGAACT GGGGGAATCT 
GCTGGAAAAA TAAGTCAAGA GTTCGAATCA ATGAAGCAAC AGCAAGCATC TGATGTTCAT 
GAACTGCAGC AGAAGCTCAG AACTGCTTTT ACTGAAAAAG ATGCCCTTCT CGAAACTGTG 
AATCGCCTCC AGGGAGAAAA TGAAAAGTTA CTATCTCAAC AAGAATTGGT ACCAGAACTT 
GAAAATACCA TAAAGAACCT TCAAGAAAAG AATGGAGTAT ACTTACTTAG TCTCAGTCAA 
AGAGATACCA TGTTAAAAGA ATTAGAAGGA AAGATAAATT CTCTTACTGA GGAAAAAGAT 
GATTTTATAA ATAAACTGAA AAATTCCCAT GAAGAAATGG ATAATTTCCA TAAGAAATGT 
GAAAGGGAAG AAAGATTGAT TCTTGAACTT GGGAAGAAAG TAGAGCAAAC AATCCAGTAC 
AACAGTGAAC TAGAACAAAA GGTAAATGAA TTAACAGGAG GACTAGAGGA GACTTTAAAA 
GAAAAGGATC AAAATGACCA AAAACTAGAA AAACTTATGG TTCAAATGAA AGTTCTCTCT 
GAAGACAAAG AAGTATTGTC AGCTGAAGTG AAGTCTCTTT ATGAGGAAAA CAATAAACTC 
AGTTCAGAAA AAAAACAGTT GAGTAGGGAT TTGGAGGTTT TTTTGTCTCA AAAAGAAGAT 
GTTATCCTTA AAGAACATAT TACTCAATTA GAAAAGAAAC TTCAGTTAAT GGTTGAAGAG 
CAAGATAATT TAAATAAACT GCTTGAAAAT GAGCAAGTTC AGAAGTTATT TGTTAAAACT 
CAGTTGTATG GTTTTCTTAA AGAAATGGGA TCAGAAGTTT CAGAAGACAG TGAAGAGAAA 
GATGTTGTTA ATGTCCTACA GGCAGTCGGT GAATCCTTGG CAAAAATAAA TGAGGAAAAA 
TGCAACCTGG CTTTTCAGCG TGATGAAAAA GTATTAGAGT TAGAAAAAGA GATTAAGTGC 
CTTCAAGAAG AGAGTGTAGT TCAGTGTGAA GAACTTAAGT CTTTATTGAG AGACTATGAG 
CAAGAGAAAG TTCTCTTAAG GAAAGAGTTA GAAGAAATAC AGTCAGAAAA AGAGGCCCTG 
CAGTCTGATC TTCTAGAAAT GAAGAATGCT AATGAAAAAA CAAGGCTTGA AAATCAGAAT 
CTTTTAATTC AAGTTGAAGA AGTATCTCAA ACATGTAGCA AAAGTGAAAT CCATAATGAA 
AAAGAAAAAT GTTTTATAAA GGAACATGAA AACCTAAAGC CACTACTAGA ACAAAAAGAA 
TTACGAGATA GGAGAGCAGA GTTGATACTA TTAAAGGATT CCTTAGCAAA ATCACCTTCT 
GTAAAAAATG ATCCTCTGTC TTCAGTAAAA GAGTTGGAAG AAAAAATAGG AAAAAATCGA 
CTAGAAAGGG GAAAATTATT GCTGCAGAAG AAGGAGGGAG AATTGTTGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCC2 ( NM_181453.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_852118.2 Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu108354
Clone ID Related Accession (Same CDS sequence)	NM_181453.4
Accession Version	NM_181453.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562601600000
Organism	Homo sapiens(human)
Product	GRIP and coiled-coil domain-containing protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068941.7 and AC012487.12. On Jun 2, 2019 this sequence version replaced NM_181453.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. CCDS Note: This CCDS representation does not have any full-length human transcript support, but it is supported by overlapping partial transcript evidence, including AF432211.1, AK090813.1, AK308707.1, and several tiled ESTs. This representation is also supported by studies in PMID:18946081, which functionally characterizes this protein, and by homology support in mouse (NP_081651.2, CCDS23859.1) and rat (NP_001101103.1). PMID:18243103 also describes a partial crystal structure for this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.24154.1, SRR1803614.9197.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGATC TTGTTCAAGA TGGGGTGGCT TCACCAGCTA CCCCTGGGAC CGGGAAATCT 
AAGCTGGAAA CATTGCCCAA AGAAGACCTC ATCAAGTTTG CCAAGAAACA GATGATGCTA 
ATACAGAAAG CTAAATCAAG GTGTACAGAA TTGGAGAAAG AAATTGAAGA ACTCAGATCA 
AAACCTGTTA CTGAAGGAAC TGGTGATATT ATTAAGGCAT TAACTGAACG TCTGGATGCT 
CTTCTTCTGG AAAAAGCAGA GACTGAGCAA CAGTGTCTTT CTCTGAAAAA GGAAAATATA 
AAAATGAAGC AAGAGGTTGA GGATTCTGTA ACAAAGATGG GAGATGCACA TAAGGAGTTG 
GAACAATCAC ATATAAACTA TGTGAAAGAA ATTGAAAATT TGAAAAATGA GTTGATGGCA 
GTACGTTCCA AATACAGTGA AGACAAAGCT AACTTACAAA AGCAGCTGGA AGAAGCAATG 
AATACGCAAT TAGAACTTTC AGAACAACTT AAATTTCAGA ACAACTCTGA AGATAATGTT 
AAAAAACTAC AAGAAGAGAT TGAGAAAATT AGGCCAGGCT TTGAGGAGCA AATTTTATAT 
CTGCAAAAGC AATTAGACGC TACCACTGAT GAAAAGAAGG AAACAGTTAC TCAACTCCAA 
AATATCATTG AGGCTAATTC TCAGCATTAC CAAAAAAATA TTAATAGTTT GCAGGAAGAG 
CTTTTACAGT TGAAAGCTAT ACACCAAGAA GAGGTGAAAG AGTTGATGTG CCAGATTGAA 
GCATCAGCTA AGGAACATGA AGCAGAGATA AATAAGTTGA ACGAGCTAAA AGAGAACTTA 
GTAAAACAAT GTGAGGCAAG TGAAAAGAAC ATCCAGAAGA AATATGAATG TGAGTTAGAA 
AATTTAAGGA AAGCCACCTC AAATGCAAAC CAAGACAATC AGATATGTTC TATTCTCTTG 
CAAGAAAATA CATTTGTAGA ACAAGTAGTA AATGAAAAAG TCAAACACTT AGAAGATACC 
TTAAAAGAAC TTGAATCTCA ACACAGTATC TTAAAAGATG AGGTAACTTA TATGAATAAT 
CTTAAGTTAA AACTTGAAAT GGATGCTCAA CATATAAAGG ATGAGTTTTT TCATGAACGG 
GAAGACTTAG AGTTTAAAAT TAATGAATTA TTACTAGCTA AAGAAGAACA GGGCTGTGTA 
ATTGAAAAAT TAAAATCTGA GCTAGCAGGT TTAAATAAAC AGTTTTGCTA TACTGTAGAA 
CAGCATAACA GAGAAGTACA GAGTCTTAAG GAACAACATC AAAAAGAAAT ATCAGAACTA 
AATGAGACAT TTTTGTCAGA TTCAGAAAAA GAAAAATTAA CATTAATGTT TGAAATACAG 
GGTCTTAAGG AACAGTGTGA AAACCTACAG CAAGAAAAGC AAGAAGCAAT TTTAAATTAT 
GAGAGTTTAC GAGAGATTAT GGAAATTTTA CAAACAGAAC TGGGGGAATC TGCTGGAAAA 
ATAAGTCAAG AGTTCGAATC AATGAAGCAA CAGCAAGCAT CTGATGTTCA TGAACTGCAG 
CAGAAGCTCA GAACTGCTTT TACTGAAAAA GATGCCCTTC TCGAAACTGT GAATCGCCTC 
CAGGGAGAAA ATGAAAAGTT ACTATCTCAA CAAGAATTGG TACCAGAACT TGAAAATACC 
ATAAAGAACC TTCAAGAAAA GAATGGAGTA TACTTACTTA GTCTCAGTCA AAGAGATACC 
ATGTTAAAAG AATTAGAAGG AAAGATAAAT TCTCTTACTG AGGAAAAAGA TGATTTTATA 
AATAAACTGA AAAATTCCCA TGAAGAAATG GATAATTTCC ATAAGAAATG TGAAAGGGAA 
GAAAGATTGA TTCTTGAACT TGGGAAGAAA GTAGAGCAAA CAATCCAGTA CAACAGTGAA 
CTAGAACAAA AGGTAAATGA ATTAACAGGA GGACTAGAGG AGACTTTAAA AGAAAAGGAT 
CAAAATGACC AAAAACTAGA AAAACTTATG GTTCAAATGA AAGTTCTCTC TGAAGACAAA 
GAAGTATTGT CAGCTGAAGT GAAGTCTCTT TATGAGGAAA ACAATAAACT CAGTTCAGAA 
AAAAAACAGT TGAGTAGGGA TTTGGAGGTT TTTTTGTCTC AAAAAGAAGA TGTTATCCTT 
AAAGAACATA TTACTCAATT AGAAAAGAAA CTTCAGTTAA TGGTTGAAGA GCAAGATAAT 
TTAAATAAAC TGCTTGAAAA TGAGCAAGTT CAGAAGTTAT TTGTTAAAAC TCAGTTGTAT 
GGTTTTCTTA AAGAAATGGG ATCAGAAGTT TCAGAAGACA GTGAAGAGAA AGATGTTGTT 
AATGTCCTAC AGGCAGTCGG TGAATCCTTG GCAAAAATAA ATGAGGAAAA ATGCAACCTG 
GCTTTTCAGC GTGATGAAAA AGTATTAGAG TTAGAAAAAG AGATTAAGTG CCTTCAAGAA 
GAGAGTGTAG TTCAGTGTGA AGAACTTAAG TCTTTATTGA GAGACTATGA GCAAGAGAAA 
GTTCTCTTAA GGAAAGAGTT AGAAGAAATA CAGTCAGAAA AAGAGGCCCT GCAGTCTGAT 
CTTCTAGAAA TGAAGAATGC TAATGAAAAA ACAAGGCTTG AAAATCAGAA TCTTTTAATT 
CAAGTTGAAG AAGTATCTCA AACATGTAGC AAAAGTGAAA TCCATAATGA AAAAGAAAAA 
TGTTTTATAA AGGAACATGA AAACCTAAAG CCACTACTAG AACAAAAAGA ATTACGAGAT 
AGGAGAGCAG AGTTGATACT ATTAAAGGAT TCCTTAGCAA AATCACCTTC TGTAAAAAAT 
GATCCTCTGT CTTCAGTAAA AGAGTTGGAA GAAAAAATAG AAAATCTGGA AAAAGAATGC 
AAAGAAAAGG AGGAGAAAAT AAATAAGATA AAATTAGTTG CCGTAAAGGC AAAGAAAGAA 
CTAGATTCCA GCAGAAAAGA GACCCAGACT GTGAAGGAAG AACTTGAATC TCTTCGATCA 
GAAAAGGACC AGTTATCTGC TTCCATGAGA GATCTCATTC AAGGAGCAGA AAGCTATAAG 
AATCTTTTAT TAGAATATGA AAAGCAGTCA GAGCAACTGG ATGTGGAAAA AGAACGTGCT 
AATAATTTTG AGCATCGTAT TGAAGACCTT ACAAGACAAT TAAGAAATTC GACTTTGCAG 
TGTGAAACAA TAAATTCTGA TAATGAAGAT CTCCTGGCTC GTATTGAGAC ATTACAGTCT 
AATGCCAAAT TATTAGAAGT ACAGATTTTA GAAGTCCAGA GAGCCAAAGC AATGGTAGAC 
AAAGAATTAG AAGCTGAAAA ACTTCAGAAA GAACAGAAGA TAAAGGAACA TGCCACTACT 
GTAAATGAAC TTGAAGAACT TCAGGTACAA CTTCAAAAGC AAAAGAAACA GCTTCAGAAA 
ACCATGCAAG AATTAGAGCT GGTTAAAAAG GATGCCCAAC AAACCACATT GATGAATATG 
GAAATAGCTG ATTATGAACG TTTGATGAAA GAACTAAATC AAAAGTTAAC TAATAAAAAC 
AACAAGATAG AAGATTTGGA GCAAGAAATA AAAATTCAAA AACAGAAACA AGAAACCCTA 
CAAGAAGAAA TAACTTCATT ACAGTCTTCA GTACAACAAT ATGAAGAAAA AAACACCAAA 
ATCAAGCAAT TGCTTGTGAA AACCAAAAAG GAACTGGCAG ATTCAAAGCA AGCAGAAACT 
GATCACTTAA TACTTCAAGC ATCTTTAAAA GGTGAGCTGG AGGCAAGCCA GCAGCAAGTA 
GAAGTCTATA AAATACAGCT GGCTGAAATA ACATCAGAGA AGCACAAAAT CCACGAGCAC 
CTGAAAACCT CTGCGGAACA GCACCAGCGT ACGCTAAGTG CATACCAGCA GAGAGTGACA 
GCACTACAGG AAGAGTGCCG TGCTGCCAAG GCAGAACAAG CTACTGTAAC CTCTGAATTC 
GAGAGCTACA AAGTCCGAGT TCATAATGTT CTAAAACAAC AGAAAAATAA ATCTATGTCT 
CAGGCTGAAA CTGAGGGCGC TAAACAAGAA AGGGAACATC TGGAAATGCT GATTGACCAG 
CTAAAAATCA AATTACAAGA TAGCCAAAAT AACTTACAGA TTAATGTATC TGAACTTCAA 
ACATTGCAGT CTGAACATGA TACACTGCTA GAAAGGCACA ACAAGATGCT GCAGGAAACT 
GTGTCCAAAG AGGCGGAACT CCGGGAAAAA TTGTGTTCAA TACAGTCAGA GAACATGATG 
ATGAAATCTG AACATACACA GACTGTGAGT CAGCTAACAT CCCAGAACGA GGTCCTTCGA 
AATAGCTTCC GAGATCAAGT GCGACATTTG CAGGAAGAAC ACAGAAAGAC AGTGGAGACA 
TTACAGCAGC AGCTCTCCAA GATGGAAGCA CAGCTCTTCC AGCTTAAGAA TGAACCGACC 
ACAAGAAGCC CAGTTTCCTC TCAACAATCT TTGAAGAACC TTCGAGAAAG GAGAAACACA 
GACCTCCCGC TTCTAGACAT GCACACTGTA ACCCGGGAAG AGGGAGAAGG CATGGAGACA 
ACTGATACGG AGTCTGTGTC TTCCGCCAGC ACATACACAC AGTCTTTAGA GCAGCTGCTT 
AACTCTCCCG AAACTAAACT TGAGCCTCCA TTATGGCATG CTGAATTTAC CAAAGAAGAA 
TTGGTTCAGA AGCTCAGTTC CACCACAAAA AGTGCAGATC ACTTAAACGG CCTGCTTCGG 
GAAACAGAAG CAACCAATGC AATTCTTATG GAGCAAATTA AGCTTCTCAA AAGTGAAATA 
AGAAGATTGG AAAGGAATCA AGAGCGAGAG AAGTCTGCAG CTAACCTGGA ATACTTGAAG 
AACGTCTTGC TGCAGTTCAT TTTCTTGAAA CCAGGTAGTG AAAGAGAGAG ACTTCTTCCT 
GTTATAAATA CGATGTTGCA GCTCAGCCCT GAAGAAAAGG GAAAACTTGC TGCGGTTGCT 
CAAGGTGAGG AAGAAAATGC TTCCCGTTCT TCTGGATGGG CATCCTATCT TCATAGTTGG 
TCTGGACTTC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852118.2
CDS	70..5124
Translation	MEDLVQDGVASPATPGTGKSKLETLPKEDLIKFAKKQMMLIQKAKSRCTELEKEIEELRSKPVTEGTGDIIKALTERLDALLLEKAETEQQCLSLKKENIKMKQEVEDSVTKMGDAHKELEQSHINYVKEIENLKNELMAVRSKYSEDKANLQKQLEEAMNTQLELSEQLKFQNNSEDNVKKLQEEIEKIRPGFEEQILYLQKQLDATTDEKKETVTQLQNIIEANSQHYQKNINSLQEELLQLKAIHQEEVKELMCQIEASAKEHEAEINKLNELKENLVKQCEASEKNIQKKYECELENLRKATSNANQDNQICSILLQENTFVEQVVNEKVKHLEDTLKELESQHSILKDEVTYMNNLKLKLEMDAQHIKDEFFHEREDLEFKINELLLAKEEQGCVIEKLKSELAGLNKQFCYTVEQHNREVQSLKEQHQKEISELNETFLSDSEKEKLTLMFEIQGLKEQCENLQQEKQEAILNYESLREIMEILQTELGESAGKISQEFESMKQQQASDVHELQQKLRTAFTEKDALLETVNRLQGENEKLLSQQELVPELENTIKNLQEKNGVYLLSLSQRDTMLKELEGKINSLTEEKDDFINKLKNSHEEMDNFHKKCEREERLILELGKKVEQTIQYNSELEQKVNELTGGLEETLKEKDQNDQKLEKLMVQMKVLSEDKEVLSAEVKSLYEENNKLSSEKKQLSRDLEVFLSQKEDVILKEHITQLEKKLQLMVEEQDNLNKLLENEQVQKLFVKTQLYGFLKEMGSEVSEDSEEKDVVNVLQAVGESLAKINEEKCNLAFQRDEKVLELEKEIKCLQEESVVQCEELKSLLRDYEQEKVLLRKELEEIQSEKEALQSDLLEMKNANEKTRLENQNLLIQVEEVSQTCSKSEIHNEKEKCFIKEHENLKPLLEQKELRDRRAELILLKDSLAKSPSVKNDPLSSVKELEEKIENLEKECKEKEEKINKIKLVAVKAKKELDSSRKETQTVKEELESLRSEKDQLSASMRDLIQGAESYKNLLLEYEKQSEQLDVEKERANNFEHRIEDLTRQLRNSTLQCETINSDNEDLLARIETLQSNAKLLEVQILEVQRAKAMVDKELEAEKLQKEQKIKEHATTVNELEELQVQLQKQKKQLQKTMQELELVKKDAQQTTLMNMEIADYERLMKELNQKLTNKNNKIEDLEQEIKIQKQKQETLQEEITSLQSSVQQYEEKNTKIKQLLVKTKKELADSKQAETDHLILQASLKGELEASQQQVEVYKIQLAEITSEKHKIHEHLKTSAEQHQRTLSAYQQRVTALQEECRAAKAEQATVTSEFESYKVRVHNVLKQQKNKSMSQAETEGAKQEREHLEMLIDQLKIKLQDSQNNLQINVSELQTLQSEHDTLLERHNKMLQETVSKEAELREKLCSIQSENMMMKSEHTQTVSQLTSQNEVLRNSFRDQVRHLQEEHRKTVETLQQQLSKMEAQLFQLKNEPTTRSPVSSQQSLKNLRERRNTDLPLLDMHTVTREEGEGMETTDTESVSSASTYTQSLEQLLNSPETKLEPPLWHAEFTKEELVQKLSSTTKSADHLNGLLRETEATNAILMEQIKLLKSEIRRLERNQEREKSAANLEYLKNVLLQFIFLKPGSERERLLPVINTMLQLSPEEKGKLAAVAQGEEENASRSSGWASYLHSWSGLR

Target ORF information:

RefSeq Version	NM_181453.4
Organism	Homo sapiens(human)
Definition	Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181453.4

ORF Insert Sequence:

ATGGAGGATC TTGTTCAAGA TGGGGTGGCT TCACCAGCTA CCCCTGGGAC CGGGAAATCT 
AAGCTGGAAA CATTGCCCAA AGAAGACCTC ATCAAGTTTG CCAAGAAACA GATGATGCTA 
ATACAGAAAG CTAAATCAAG GTGTACAGAA TTGGAGAAAG AAATTGAAGA ACTCAGATCA 
AAACCTGTTA CTGAAGGAAC TGGTGATATT ATTAAGGCAT TAACTGAACG TCTGGATGCT 
CTTCTTCTGG AAAAAGCAGA GACTGAGCAA CAGTGTCTTT CTCTGAAAAA GGAAAATATA 
AAAATGAAGC AAGAGGTTGA GGATTCTGTA ACAAAGATGG GAGATGCACA TAAGGAGTTG 
GAACAATCAC ATATAAACTA TGTGAAAGAA ATTGAAAATT TGAAAAATGA GTTGATGGCA 
GTACGTTCCA AATACAGTGA AGACAAAGCT AACTTACAAA AGCAGCTGGA AGAAGCAATG 
AATACGCAAT TAGAACTTTC AGAACAACTT AAATTTCAGA ACAACTCTGA AGATAATGTT 
AAAAAACTAC AAGAAGAGAT TGAGAAAATT AGGCCAGGCT TTGAGGAGCA AATTTTATAT 
CTGCAAAAGC AATTAGACGC TACCACTGAT GAAAAGAAGG AAACAGTTAC TCAACTCCAA 
AATATCATTG AGGCTAATTC TCAGCATTAC CAAAAAAATA TTAATAGTTT GCAGGAAGAG 
CTTTTACAGT TGAAAGCTAT ACACCAAGAA GAGGTGAAAG AGTTGATGTG CCAGATTGAA 
GCATCAGCTA AGGAACATGA AGCAGAGATA AATAAGTTGA ACGAGCTAAA AGAGAACTTA 
GTAAAACAAT GTGAGGCAAG TGAAAAGAAC ATCCAGAAGA AATATGAATG TGAGTTAGAA 
AATTTAAGGA AAGCCACCTC AAATGCAAAC CAAGACAATC AGATATGTTC TATTCTCTTG 
CAAGAAAATA CATTTGTAGA ACAAGTAGTA AATGAAAAAG TCAAACACTT AGAAGATACC 
TTAAAAGAAC TTGAATCTCA ACACAGTATC TTAAAAGATG AGGTAACTTA TATGAATAAT 
CTTAAGTTAA AACTTGAAAT GGATGCTCAA CATATAAAGG ATGAGTTTTT TCATGAACGG 
GAAGACTTAG AGTTTAAAAT TAATGAATTA TTACTAGCTA AAGAAGAACA GGGCTGTGTA 
ATTGAAAAAT TAAAATCTGA GCTAGCAGGT TTAAATAAAC AGTTTTGCTA TACTGTAGAA 
CAGCATAACA GAGAAGTACA GAGTCTTAAG GAACAACATC AAAAAGAAAT ATCAGAACTA 
AATGAGACAT TTTTGTCAGA TTCAGAAAAA GAAAAATTAA CATTAATGTT TGAAATACAG 
GGTCTTAAGG AACAGTGTGA AAACCTACAG CAAGAAAAGC AAGAAGCAAT TTTAAATTAT 
GAGAGTTTAC GAGAGATTAT GGAAATTTTA CAAACAGAAC TGGGGGAATC TGCTGGAAAA 
ATAAGTCAAG AGTTCGAATC AATGAAGCAA CAGCAAGCAT CTGATGTTCA TGAACTGCAG 
CAGAAGCTCA GAACTGCTTT TACTGAAAAA GATGCCCTTC TCGAAACTGT GAATCGCCTC 
CAGGGAGAAA ATGAAAAGTT ACTATCTCAA CAAGAATTGG TACCAGAACT TGAAAATACC 
ATAAAGAACC TTCAAGAAAA GAATGGAGTA TACTTACTTA GTCTCAGTCA AAGAGATACC 
ATGTTAAAAG AATTAGAAGG AAAGATAAAT TCTCTTACTG AGGAAAAAGA TGATTTTATA 
AATAAACTGA AAAATTCCCA TGAAGAAATG GATAATTTCC ATAAGAAATG TGAAAGGGAA 
GAAAGATTGA TTCTTGAACT TGGGAAGAAA GTAGAGCAAA CAATCCAGTA CAACAGTGAA 
CTAGAACAAA AGGTAAATGA ATTAACAGGA GGACTAGAGG AGACTTTAAA AGAAAAGGAT 
CAAAATGACC AAAAACTAGA AAAACTTATG GTTCAAATGA AAGTTCTCTC TGAAGACAAA 
GAAGTATTGT CAGCTGAAGT GAAGTCTCTT TATGAGGAAA ACAATAAACT CAGTTCAGAA 
AAAAAACAGT TGAGTAGGGA TTTGGAGGTT TTTTTGTCTC AAAAAGAAGA TGTTATCCTT 
AAAGAACATA TTACTCAATT AGAAAAGAAA CTTCAGTTAA TGGTTGAAGA GCAAGATAAT 
TTAAATAAAC TGCTTGAAAA TGAGCAAGTT CAGAAGTTAT TTGTTAAAAC TCAGTTGTAT 
GGTTTTCTTA AAGAAATGGG ATCAGAAGTT TCAGAAGACA GTGAAGAGAA AGATGTTGTT 
AATGTCCTAC AGGCAGTCGG TGAATCCTTG GCAAAAATAA ATGAGGAAAA ATGCAACCTG 
GCTTTTCAGC GTGATGAAAA AGTATTAGAG TTAGAAAAAG AGATTAAGTG CCTTCAAGAA 
GAGAGTGTAG TTCAGTGTGA AGAACTTAAG TCTTTATTGA GAGACTATGA GCAAGAGAAA 
GTTCTCTTAA GGAAAGAGTT AGAAGAAATA CAGTCAGAAA AAGAGGCCCT GCAGTCTGAT 
CTTCTAGAAA TGAAGAATGC TAATGAAAAA ACAAGGCTTG AAAATCAGAA TCTTTTAATT 
CAAGTTGAAG AAGTATCTCA AACATGTAGC AAAAGTGAAA TCCATAATGA AAAAGAAAAA 
TGTTTTATAA AGGAACATGA AAACCTAAAG CCACTACTAG AACAAAAAGA ATTACGAGAT 
AGGAGAGCAG AGTTGATACT ATTAAAGGAT TCCTTAGCAA AATCACCTTC TGTAAAAAAT 
GATCCTCTGT CTTCAGTAAA AGAGTTGGAA GAAAAAATAG AAAATCTGGA AAAAGAATGC 
AAAGAAAAGG AGGAGAAAAT AAATAAGATA AAATTAGTTG CCGTAAAGGC AAAGAAAGAA 
CTAGATTCCA GCAGAAAAGA GACCCAGACT GTGAAGGAAG AACTTGAATC TCTTCGATCA 
GAAAAGGACC AGTTATCTGC TTCCATGAGA GATCTCATTC AAGGAGCAGA AAGCTATAAG 
AATCTTTTAT TAGAATATGA AAAGCAGTCA GAGCAACTGG ATGTGGAAAA AGAACGTGCT 
AATAATTTTG AGCATCGTAT TGAAGACCTT ACAAGACAAT TAAGAAATTC GACTTTGCAG 
TGTGAAACAA TAAATTCTGA TAATGAAGAT CTCCTGGCTC GTATTGAGAC ATTACAGTCT 
AATGCCAAAT TATTAGAAGT ACAGATTTTA GAAGTCCAGA GAGCCAAAGC AATGGTAGAC 
AAAGAATTAG AAGCTGAAAA ACTTCAGAAA GAACAGAAGA TAAAGGAACA TGCCACTACT 
GTAAATGAAC TTGAAGAACT TCAGGTACAA CTTCAAAAGC AAAAGAAACA GCTTCAGAAA 
ACCATGCAAG AATTAGAGCT GGTTAAAAAG GATGCCCAAC AAACCACATT GATGAATATG 
GAAATAGCTG ATTATGAACG TTTGATGAAA GAACTAAATC AAAAGTTAAC TAATAAAAAC 
AACAAGATAG AAGATTTGGA GCAAGAAATA AAAATTCAAA AACAGAAACA AGAAACCCTA 
CAAGAAGAAA TAACTTCATT ACAGTCTTCA GTACAACAAT ATGAAGAAAA AAACACCAAA 
ATCAAGCAAT TGCTTGTGAA AACCAAAAAG GAACTGGCAG ATTCAAAGCA AGCAGAAACT 
GATCACTTAA TACTTCAAGC ATCTTTAAAA GGTGAGCTGG AGGCAAGCCA GCAGCAAGTA 
GAAGTCTATA AAATACAGCT GGCTGAAATA ACATCAGAGA AGCACAAAAT CCACGAGCAC 
CTGAAAACCT CTGCGGAACA GCACCAGCGT ACGCTAAGTG CATACCAGCA GAGAGTGACA 
GCACTACAGG AAGAGTGCCG TGCTGCCAAG GCAGAACAAG CTACTGTAAC CTCTGAATTC 
GAGAGCTACA AAGTCCGAGT TCATAATGTT CTAAAACAAC AGAAAAATAA ATCTATGTCT 
CAGGCTGAAA CTGAGGGCGC TAAACAAGAA AGGGAACATC TGGAAATGCT GATTGACCAG 
CTAAAAATCA AATTACAAGA TAGCCAAAAT AACTTACAGA TTAATGTATC TGAACTTCAA 
ACATTGCAGT CTGAACATGA TACACTGCTA GAAAGGCACA ACAAGATGCT GCAGGAAACT 
GTGTCCAAAG AGGCGGAACT CCGGGAAAAA TTGTGTTCAA TACAGTCAGA GAACATGATG 
ATGAAATCTG AACATACACA GACTGTGAGT CAGCTAACAT CCCAGAACGA GGTCCTTCGA 
AATAGCTTCC GAGATCAAGT GCGACATTTG CAGGAAGAAC ACAGAAAGAC AGTGGAGACA 
TTACAGCAGC AGCTCTCCAA GATGGAAGCA CAGCTCTTCC AGCTTAAGAA TGAACCGACC 
ACAAGAAGCC CAGTTTCCTC TCAACAATCT TTGAAGAACC TTCGAGAAAG GAGAAACACA 
GACCTCCCGC TTCTAGACAT GCACACTGTA ACCCGGGAAG AGGGAGAAGG CATGGAGACA 
ACTGATACGG AGTCTGTGTC TTCCGCCAGC ACATACACAC AGTCTTTAGA GCAGCTGCTT 
AACTCTCCCG AAACTAAACT TGAGCCTCCA TTATGGCATG CTGAATTTAC CAAAGAAGAA 
TTGGTTCAGA AGCTCAGTTC CACCACAAAA AGTGCAGATC ACTTAAACGG CCTGCTTCGG 
GAAACAGAAG CAACCAATGC AATTCTTATG GAGCAAATTA AGCTTCTCAA AAGTGAAATA 
AGAAGATTGG AAAGGAATCA AGAGCGAGAG AAGTCTGCAG CTAACCTGGA ATACTTGAAG 
AACGTCTTGC TGCAGTTCAT TTTCTTGAAA CCAGGTAGTG AAAGAGAGAG ACTTCTTCCT 
GTTATAAATA CGATGTTGCA GCTCAGCCCT GAAGAAAAGG GAAAACTTGC TGCGGTTGCT 
CAAGGTGAGG AAGAAAATGC TTCCCGTTCT TCTGGATGGG CATCCTATCT TCATAGTTGG 
TCTGGACTTC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCC2 ( NM_181453.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_852118.1 Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant 1, mRNA.

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CloneID	OHu31795
Clone ID Related Accession (Same CDS sequence)	NM_181453.3
Accession Version	NM_181453.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550678400000
Organism	Homo sapiens(human)
Product	GRIP and coiled-coil domain-containing protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK090813.1, BC146789.1, AA171957.1, BM694019.1 and AI084646.1. On or before Nov 2, 2012 this sequence version replaced XM_003960914.1, XM_003960915.1, XM_003960916.1, XM_003960917.1, XM_003960918.1, XM_003960919.1, XM_003960920.1, NM_181453.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. CCDS Note: This CCDS representation does not have any full-length human transcript support, but it is supported by overlapping partial transcript evidence, including AF432211.1, AK090813.1, AK308707.1, and several tiled ESTs. This representation is also supported by studies in PMID:18946081, which functionally characterizes this protein, and by homology support in mouse (NP_081651.2, CCDS23859.1) and rat (NP_001101103.1). PMID:18243103 also describes a partial crystal structure for this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.37825.1, SRR1660805.114312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGATC TTGTTCAAGA TGGGGTGGCT TCACCAGCTA CCCCTGGGAC CGGGAAATCT 
AAGCTGGAAA CATTGCCCAA AGAAGACCTC ATCAAGTTTG CCAAGAAACA GATGATGCTA 
ATACAGAAAG CTAAATCAAG GTGTACAGAA TTGGAGAAAG AAATTGAAGA ACTCAGATCA 
AAACCTGTTA CTGAAGGAAC TGGTGATATT ATTAAGGCAT TAACTGAACG TCTGGATGCT 
CTTCTTCTGG AAAAAGCAGA GACTGAGCAA CAGTGTCTTT CTCTGAAAAA GGAAAATATA 
AAAATGAAGC AAGAGGTTGA GGATTCTGTA ACAAAGATGG GAGATGCACA TAAGGAGTTG 
GAACAATCAC ATATAAACTA TGTGAAAGAA ATTGAAAATT TGAAAAATGA GTTGATGGCA 
GTACGTTCCA AATACAGTGA AGACAAAGCT AACTTACAAA AGCAGCTGGA AGAAGCAATG 
AATACGCAAT TAGAACTTTC AGAACAACTT AAATTTCAGA ACAACTCTGA AGATAATGTT 
AAAAAACTAC AAGAAGAGAT TGAGAAAATT AGGCCAGGCT TTGAGGAGCA AATTTTATAT 
CTGCAAAAGC AATTAGACGC TACCACTGAT GAAAAGAAGG AAACAGTTAC TCAACTCCAA 
AATATCATTG AGGCTAATTC TCAGCATTAC CAAAAAAATA TTAATAGTTT GCAGGAAGAG 
CTTTTACAGT TGAAAGCTAT ACACCAAGAA GAGGTGAAAG AGTTGATGTG CCAGATTGAA 
GCATCAGCTA AGGAACATGA AGCAGAGATA AATAAGTTGA ACGAGCTAAA AGAGAACTTA 
GTAAAACAAT GTGAGGCAAG TGAAAAGAAC ATCCAGAAGA AATATGAATG TGAGTTAGAA 
AATTTAAGGA AAGCCACCTC AAATGCAAAC CAAGACAATC AGATATGTTC TATTCTCTTG 
CAAGAAAATA CATTTGTAGA ACAAGTAGTA AATGAAAAAG TCAAACACTT AGAAGATACC 
TTAAAAGAAC TTGAATCTCA ACACAGTATC TTAAAAGATG AGGTAACTTA TATGAATAAT 
CTTAAGTTAA AACTTGAAAT GGATGCTCAA CATATAAAGG ATGAGTTTTT TCATGAACGG 
GAAGACTTAG AGTTTAAAAT TAATGAATTA TTACTAGCTA AAGAAGAACA GGGCTGTGTA 
ATTGAAAAAT TAAAATCTGA GCTAGCAGGT TTAAATAAAC AGTTTTGCTA TACTGTAGAA 
CAGCATAACA GAGAAGTACA GAGTCTTAAG GAACAACATC AAAAAGAAAT ATCAGAACTA 
AATGAGACAT TTTTGTCAGA TTCAGAAAAA GAAAAATTAA CATTAATGTT TGAAATACAG 
GGTCTTAAGG AACAGTGTGA AAACCTACAG CAAGAAAAGC AAGAAGCAAT TTTAAATTAT 
GAGAGTTTAC GAGAGATTAT GGAAATTTTA CAAACAGAAC TGGGGGAATC TGCTGGAAAA 
ATAAGTCAAG AGTTCGAATC AATGAAGCAA CAGCAAGCAT CTGATGTTCA TGAACTGCAG 
CAGAAGCTCA GAACTGCTTT TACTGAAAAA GATGCCCTTC TCGAAACTGT GAATCGCCTC 
CAGGGAGAAA ATGAAAAGTT ACTATCTCAA CAAGAATTGG TACCAGAACT TGAAAATACC 
ATAAAGAACC TTCAAGAAAA GAATGGAGTA TACTTACTTA GTCTCAGTCA AAGAGATACC 
ATGTTAAAAG AATTAGAAGG AAAGATAAAT TCTCTTACTG AGGAAAAAGA TGATTTTATA 
AATAAACTGA AAAATTCCCA TGAAGAAATG GATAATTTCC ATAAGAAATG TGAAAGGGAA 
GAAAGATTGA TTCTTGAACT TGGGAAGAAA GTAGAGCAAA CAATCCAGTA CAACAGTGAA 
CTAGAACAAA AGGTAAATGA ATTAACAGGA GGACTAGAGG AGACTTTAAA AGAAAAGGAT 
CAAAATGACC AAAAACTAGA AAAACTTATG GTTCAAATGA AAGTTCTCTC TGAAGACAAA 
GAAGTATTGT CAGCTGAAGT GAAGTCTCTT TATGAGGAAA ACAATAAACT CAGTTCAGAA 
AAAAAACAGT TGAGTAGGGA TTTGGAGGTT TTTTTGTCTC AAAAAGAAGA TGTTATCCTT 
AAAGAACATA TTACTCAATT AGAAAAGAAA CTTCAGTTAA TGGTTGAAGA GCAAGATAAT 
TTAAATAAAC TGCTTGAAAA TGAGCAAGTT CAGAAGTTAT TTGTTAAAAC TCAGTTGTAT 
GGTTTTCTTA AAGAAATGGG ATCAGAAGTT TCAGAAGACA GTGAAGAGAA AGATGTTGTT 
AATGTCCTAC AGGCAGTCGG TGAATCCTTG GCAAAAATAA ATGAGGAAAA ATGCAACCTG 
GCTTTTCAGC GTGATGAAAA AGTATTAGAG TTAGAAAAAG AGATTAAGTG CCTTCAAGAA 
GAGAGTGTAG TTCAGTGTGA AGAACTTAAG TCTTTATTGA GAGACTATGA GCAAGAGAAA 
GTTCTCTTAA GGAAAGAGTT AGAAGAAATA CAGTCAGAAA AAGAGGCCCT GCAGTCTGAT 
CTTCTAGAAA TGAAGAATGC TAATGAAAAA ACAAGGCTTG AAAATCAGAA TCTTTTAATT 
CAAGTTGAAG AAGTATCTCA AACATGTAGC AAAAGTGAAA TCCATAATGA AAAAGAAAAA 
TGTTTTATAA AGGAACATGA AAACCTAAAG CCACTACTAG AACAAAAAGA ATTACGAGAT 
AGGAGAGCAG AGTTGATACT ATTAAAGGAT TCCTTAGCAA AATCACCTTC TGTAAAAAAT 
GATCCTCTGT CTTCAGTAAA AGAGTTGGAA GAAAAAATAG AAAATCTGGA AAAAGAATGC 
AAAGAAAAGG AGGAGAAAAT AAATAAGATA AAATTAGTTG CCGTAAAGGC AAAGAAAGAA 
CTAGATTCCA GCAGAAAAGA GACCCAGACT GTGAAGGAAG AACTTGAATC TCTTCGATCA 
GAAAAGGACC AGTTATCTGC TTCCATGAGA GATCTCATTC AAGGAGCAGA AAGCTATAAG 
AATCTTTTAT TAGAATATGA AAAGCAGTCA GAGCAACTGG ATGTGGAAAA AGAACGTGCT 
AATAATTTTG AGCATCGTAT TGAAGACCTT ACAAGACAAT TAAGAAATTC GACTTTGCAG 
TGTGAAACAA TAAATTCTGA TAATGAAGAT CTCCTGGCTC GTATTGAGAC ATTACAGTCT 
AATGCCAAAT TATTAGAAGT ACAGATTTTA GAAGTCCAGA GAGCCAAAGC AATGGTAGAC 
AAAGAATTAG AAGCTGAAAA ACTTCAGAAA GAACAGAAGA TAAAGGAACA TGCCACTACT 
GTAAATGAAC TTGAAGAACT TCAGGTACAA CTTCAAAAGG AAAAGAAACA GCTTCAGAAA 
ACCATGCAAG AATTAGAGCT GGTTAAAAAG GATGCCCAAC AAACCACATT GATGAATATG 
GAAATAGCTG ATTATGAACG TTTGATGAAA GAACTAAATC AAAAGTTAAC TAATAAAAAC 
AACAAGATAG AAGATTTGGA GCAAGAAATA AAAATTCAAA AACAGAAACA AGAAACCCTA 
CAAGAAGAAA TAACTTCATT ACAGTCTTCA GTACAACAAT ATGAAGAAAA AAACACCAAA 
ATCAAGCAAT TGCTTGTGAA AACCAAAAAG GAACTGGCAG ATTCAAAGCA AGCAGAAACT 
GATCACTTAA TACTTCAAGC ATCTTTAAAA GGTGAGCTGG AGGCAAGCCA GCAGCAAGTA 
GAAGTCTATA AAATACAGCT GGCTGAAATA ACATCAGAGA AGCACAAAAT CCACGAGCAC 
CTGAAAACCT CTGCGGAACA GCACCAGCGT ACGCTAAGTG CATACCAGCA GAGAGTGACA 
GCACTACAGG AAGAGTGCCG TGCTGCCAAG GCAGAACAAG CTACTGTAAC CTCTGAATTC 
GAGAGCTACA AAGTCCGAGT TCATAATGTT CTAAAACAAC AGAAAAATAA ATCTATGTCT 
CAGGCTGAAA CTGAGGGCGC TAAACAAGAA AGGGAACATC TGGAAATGCT GATTGACCAG 
CTAAAAATCA AATTACAAGA TAGCCAAAAT AACTTACAGA TTAATGTATC TGAACTTCAA 
ACATTGCAGT CTGAACATGA TACACTGCTA GAAAGGCACA ACAAGATGCT GCAGGAAACT 
GTGTCCAAAG AGGCGGAACT CCGGGAAAAA TTGTGTTCAA TACAGTCAGA GAACATGATG 
ATGAAATCTG AACATACACA GACTGTGAGT CAGCTAACAT CCCAGAACGA GGTCCTTCGA 
AATAGCTTCC GAGATCAAGT GCGACATTTG CAGGAAGAAC ACAGAAAGAC AGTGGAGACA 
TTACAGCAGC AGCTCTCCAA GATGGAAGCA CAGCTCTTCC AGCTTAAGAA TGAACCGACC 
ACAAGAAGCC CAGTTTCCTC TCAACAATCT TTGAAGAACC TTCGAGAAAG GAGAAACACA 
GACCTCCCGC TTCTAGACAT GCACACTGTA ACCCGGGAAG AGGGAGAAGG CATGGAGACA 
ACTGATACGG AGTCTGTGTC TTCCGCCAGC ACATACACAC AGTCTTTAGA GCAGCTGCTT 
AACTCTCCCG AAACTAAACT TGAGCCTCCA TTATGGCATG CTGAATTTAC CAAAGAAGAA 
TTGGTTCAGA AGCTCAGTTC CACCACAAAA AGTGCAGATC ACTTAAACGG CCTGCTTCGG 
GAAACAGAAG CAACCAATGC AATTCTTATG GAGCAAATTA AGCTTCTCAA AAGTGAAATA 
AGAAGATTGG AAAGGAATCA AGAGCGAGAG AAGTCTGCAG CTAACCTGGA ATACTTGAAG 
AACGTCTTGC TGCAGTTCAT TTTCTTGAAA CCAGGTAGTG AAAGAGAGAG ACTTCTTCCT 
GTTATAAATA CGATGTTGCA GCTCAGCCCT GAAGAAAAGG GAAAACTTGC TGCGGTTGCT 
CAAGGTGAGG AAGAAAATGC TTCCCGTTCT TCTGGATGGG CATCCTATCT TCATAGTTGG 
TCTGGACTTC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852118.1
CDS	155..5209
Translation	MEDLVQDGVASPATPGTGKSKLETLPKEDLIKFAKKQMMLIQKAKSRCTELEKEIEELRSKPVTEGTGDIIKALTERLDALLLEKAETEQQCLSLKKENIKMKQEVEDSVTKMGDAHKELEQSHINYVKEIENLKNELMAVRSKYSEDKANLQKQLEEAMNTQLELSEQLKFQNNSEDNVKKLQEEIEKIRPGFEEQILYLQKQLDATTDEKKETVTQLQNIIEANSQHYQKNINSLQEELLQLKAIHQEEVKELMCQIEASAKEHEAEINKLNELKENLVKQCEASEKNIQKKYECELENLRKATSNANQDNQICSILLQENTFVEQVVNEKVKHLEDTLKELESQHSILKDEVTYMNNLKLKLEMDAQHIKDEFFHEREDLEFKINELLLAKEEQGCVIEKLKSELAGLNKQFCYTVEQHNREVQSLKEQHQKEISELNETFLSDSEKEKLTLMFEIQGLKEQCENLQQEKQEAILNYESLREIMEILQTELGESAGKISQEFESMKQQQASDVHELQQKLRTAFTEKDALLETVNRLQGENEKLLSQQELVPELENTIKNLQEKNGVYLLSLSQRDTMLKELEGKINSLTEEKDDFINKLKNSHEEMDNFHKKCEREERLILELGKKVEQTIQYNSELEQKVNELTGGLEETLKEKDQNDQKLEKLMVQMKVLSEDKEVLSAEVKSLYEENNKLSSEKKQLSRDLEVFLSQKEDVILKEHITQLEKKLQLMVEEQDNLNKLLENEQVQKLFVKTQLYGFLKEMGSEVSEDSEEKDVVNVLQAVGESLAKINEEKCNLAFQRDEKVLELEKEIKCLQEESVVQCEELKSLLRDYEQEKVLLRKELEEIQSEKEALQSDLLEMKNANEKTRLENQNLLIQVEEVSQTCSKSEIHNEKEKCFIKEHENLKPLLEQKELRDRRAELILLKDSLAKSPSVKNDPLSSVKELEEKIENLEKECKEKEEKINKIKLVAVKAKKELDSSRKETQTVKEELESLRSEKDQLSASMRDLIQGAESYKNLLLEYEKQSEQLDVEKERANNFEHRIEDLTRQLRNSTLQCETINSDNEDLLARIETLQSNAKLLEVQILEVQRAKAMVDKELEAEKLQKEQKIKEHATTVNELEELQVQLQKEKKQLQKTMQELELVKKDAQQTTLMNMEIADYERLMKELNQKLTNKNNKIEDLEQEIKIQKQKQETLQEEITSLQSSVQQYEEKNTKIKQLLVKTKKELADSKQAETDHLILQASLKGELEASQQQVEVYKIQLAEITSEKHKIHEHLKTSAEQHQRTLSAYQQRVTALQEECRAAKAEQATVTSEFESYKVRVHNVLKQQKNKSMSQAETEGAKQEREHLEMLIDQLKIKLQDSQNNLQINVSELQTLQSEHDTLLERHNKMLQETVSKEAELREKLCSIQSENMMMKSEHTQTVSQLTSQNEVLRNSFRDQVRHLQEEHRKTVETLQQQLSKMEAQLFQLKNEPTTRSPVSSQQSLKNLRERRNTDLPLLDMHTVTREEGEGMETTDTESVSSASTYTQSLEQLLNSPETKLEPPLWHAEFTKEELVQKLSSTTKSADHLNGLLRETEATNAILMEQIKLLKSEIRRLERNQEREKSAANLEYLKNVLLQFIFLKPGSERERLLPVINTMLQLSPEEKGKLAAVAQGEEENASRSSGWASYLHSWSGLR

Target ORF information:

RefSeq Version	NM_181453.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181453.3

ORF Insert Sequence:

ATGGAGGATC TTGTTCAAGA TGGGGTGGCT TCACCAGCTA CCCCTGGGAC CGGGAAATCT 
AAGCTGGAAA CATTGCCCAA AGAAGACCTC ATCAAGTTTG CCAAGAAACA GATGATGCTA 
ATACAGAAAG CTAAATCAAG GTGTACAGAA TTGGAGAAAG AAATTGAAGA ACTCAGATCA 
AAACCTGTTA CTGAAGGAAC TGGTGATATT ATTAAGGCAT TAACTGAACG TCTGGATGCT 
CTTCTTCTGG AAAAAGCAGA GACTGAGCAA CAGTGTCTTT CTCTGAAAAA GGAAAATATA 
AAAATGAAGC AAGAGGTTGA GGATTCTGTA ACAAAGATGG GAGATGCACA TAAGGAGTTG 
GAACAATCAC ATATAAACTA TGTGAAAGAA ATTGAAAATT TGAAAAATGA GTTGATGGCA 
GTACGTTCCA AATACAGTGA AGACAAAGCT AACTTACAAA AGCAGCTGGA AGAAGCAATG 
AATACGCAAT TAGAACTTTC AGAACAACTT AAATTTCAGA ACAACTCTGA AGATAATGTT 
AAAAAACTAC AAGAAGAGAT TGAGAAAATT AGGCCAGGCT TTGAGGAGCA AATTTTATAT 
CTGCAAAAGC AATTAGACGC TACCACTGAT GAAAAGAAGG AAACAGTTAC TCAACTCCAA 
AATATCATTG AGGCTAATTC TCAGCATTAC CAAAAAAATA TTAATAGTTT GCAGGAAGAG 
CTTTTACAGT TGAAAGCTAT ACACCAAGAA GAGGTGAAAG AGTTGATGTG CCAGATTGAA 
GCATCAGCTA AGGAACATGA AGCAGAGATA AATAAGTTGA ACGAGCTAAA AGAGAACTTA 
GTAAAACAAT GTGAGGCAAG TGAAAAGAAC ATCCAGAAGA AATATGAATG TGAGTTAGAA 
AATTTAAGGA AAGCCACCTC AAATGCAAAC CAAGACAATC AGATATGTTC TATTCTCTTG 
CAAGAAAATA CATTTGTAGA ACAAGTAGTA AATGAAAAAG TCAAACACTT AGAAGATACC 
TTAAAAGAAC TTGAATCTCA ACACAGTATC TTAAAAGATG AGGTAACTTA TATGAATAAT 
CTTAAGTTAA AACTTGAAAT GGATGCTCAA CATATAAAGG ATGAGTTTTT TCATGAACGG 
GAAGACTTAG AGTTTAAAAT TAATGAATTA TTACTAGCTA AAGAAGAACA GGGCTGTGTA 
ATTGAAAAAT TAAAATCTGA GCTAGCAGGT TTAAATAAAC AGTTTTGCTA TACTGTAGAA 
CAGCATAACA GAGAAGTACA GAGTCTTAAG GAACAACATC AAAAAGAAAT ATCAGAACTA 
AATGAGACAT TTTTGTCAGA TTCAGAAAAA GAAAAATTAA CATTAATGTT TGAAATACAG 
GGTCTTAAGG AACAGTGTGA AAACCTACAG CAAGAAAAGC AAGAAGCAAT TTTAAATTAT 
GAGAGTTTAC GAGAGATTAT GGAAATTTTA CAAACAGAAC TGGGGGAATC TGCTGGAAAA 
ATAAGTCAAG AGTTCGAATC AATGAAGCAA CAGCAAGCAT CTGATGTTCA TGAACTGCAG 
CAGAAGCTCA GAACTGCTTT TACTGAAAAA GATGCCCTTC TCGAAACTGT GAATCGCCTC 
CAGGGAGAAA ATGAAAAGTT ACTATCTCAA CAAGAATTGG TACCAGAACT TGAAAATACC 
ATAAAGAACC TTCAAGAAAA GAATGGAGTA TACTTACTTA GTCTCAGTCA AAGAGATACC 
ATGTTAAAAG AATTAGAAGG AAAGATAAAT TCTCTTACTG AGGAAAAAGA TGATTTTATA 
AATAAACTGA AAAATTCCCA TGAAGAAATG GATAATTTCC ATAAGAAATG TGAAAGGGAA 
GAAAGATTGA TTCTTGAACT TGGGAAGAAA GTAGAGCAAA CAATCCAGTA CAACAGTGAA 
CTAGAACAAA AGGTAAATGA ATTAACAGGA GGACTAGAGG AGACTTTAAA AGAAAAGGAT 
CAAAATGACC AAAAACTAGA AAAACTTATG GTTCAAATGA AAGTTCTCTC TGAAGACAAA 
GAAGTATTGT CAGCTGAAGT GAAGTCTCTT TATGAGGAAA ACAATAAACT CAGTTCAGAA 
AAAAAACAGT TGAGTAGGGA TTTGGAGGTT TTTTTGTCTC AAAAAGAAGA TGTTATCCTT 
AAAGAACATA TTACTCAATT AGAAAAGAAA CTTCAGTTAA TGGTTGAAGA GCAAGATAAT 
TTAAATAAAC TGCTTGAAAA TGAGCAAGTT CAGAAGTTAT TTGTTAAAAC TCAGTTGTAT 
GGTTTTCTTA AAGAAATGGG ATCAGAAGTT TCAGAAGACA GTGAAGAGAA AGATGTTGTT 
AATGTCCTAC AGGCAGTCGG TGAATCCTTG GCAAAAATAA ATGAGGAAAA ATGCAACCTG 
GCTTTTCAGC GTGATGAAAA AGTATTAGAG TTAGAAAAAG AGATTAAGTG CCTTCAAGAA 
GAGAGTGTAG TTCAGTGTGA AGAACTTAAG TCTTTATTGA GAGACTATGA GCAAGAGAAA 
GTTCTCTTAA GGAAAGAGTT AGAAGAAATA CAGTCAGAAA AAGAGGCCCT GCAGTCTGAT 
CTTCTAGAAA TGAAGAATGC TAATGAAAAA ACAAGGCTTG AAAATCAGAA TCTTTTAATT 
CAAGTTGAAG AAGTATCTCA AACATGTAGC AAAAGTGAAA TCCATAATGA AAAAGAAAAA 
TGTTTTATAA AGGAACATGA AAACCTAAAG CCACTACTAG AACAAAAAGA ATTACGAGAT 
AGGAGAGCAG AGTTGATACT ATTAAAGGAT TCCTTAGCAA AATCACCTTC TGTAAAAAAT 
GATCCTCTGT CTTCAGTAAA AGAGTTGGAA GAAAAAATAG AAAATCTGGA AAAAGAATGC 
AAAGAAAAGG AGGAGAAAAT AAATAAGATA AAATTAGTTG CCGTAAAGGC AAAGAAAGAA 
CTAGATTCCA GCAGAAAAGA GACCCAGACT GTGAAGGAAG AACTTGAATC TCTTCGATCA 
GAAAAGGACC AGTTATCTGC TTCCATGAGA GATCTCATTC AAGGAGCAGA AAGCTATAAG 
AATCTTTTAT TAGAATATGA AAAGCAGTCA GAGCAACTGG ATGTGGAAAA AGAACGTGCT 
AATAATTTTG AGCATCGTAT TGAAGACCTT ACAAGACAAT TAAGAAATTC GACTTTGCAG 
TGTGAAACAA TAAATTCTGA TAATGAAGAT CTCCTGGCTC GTATTGAGAC ATTACAGTCT 
AATGCCAAAT TATTAGAAGT ACAGATTTTA GAAGTCCAGA GAGCCAAAGC AATGGTAGAC 
AAAGAATTAG AAGCTGAAAA ACTTCAGAAA GAACAGAAGA TAAAGGAACA TGCCACTACT 
GTAAATGAAC TTGAAGAACT TCAGGTACAA CTTCAAAAGG AAAAGAAACA GCTTCAGAAA 
ACCATGCAAG AATTAGAGCT GGTTAAAAAG GATGCCCAAC AAACCACATT GATGAATATG 
GAAATAGCTG ATTATGAACG TTTGATGAAA GAACTAAATC AAAAGTTAAC TAATAAAAAC 
AACAAGATAG AAGATTTGGA GCAAGAAATA AAAATTCAAA AACAGAAACA AGAAACCCTA 
CAAGAAGAAA TAACTTCATT ACAGTCTTCA GTACAACAAT ATGAAGAAAA AAACACCAAA 
ATCAAGCAAT TGCTTGTGAA AACCAAAAAG GAACTGGCAG ATTCAAAGCA AGCAGAAACT 
GATCACTTAA TACTTCAAGC ATCTTTAAAA GGTGAGCTGG AGGCAAGCCA GCAGCAAGTA 
GAAGTCTATA AAATACAGCT GGCTGAAATA ACATCAGAGA AGCACAAAAT CCACGAGCAC 
CTGAAAACCT CTGCGGAACA GCACCAGCGT ACGCTAAGTG CATACCAGCA GAGAGTGACA 
GCACTACAGG AAGAGTGCCG TGCTGCCAAG GCAGAACAAG CTACTGTAAC CTCTGAATTC 
GAGAGCTACA AAGTCCGAGT TCATAATGTT CTAAAACAAC AGAAAAATAA ATCTATGTCT 
CAGGCTGAAA CTGAGGGCGC TAAACAAGAA AGGGAACATC TGGAAATGCT GATTGACCAG 
CTAAAAATCA AATTACAAGA TAGCCAAAAT AACTTACAGA TTAATGTATC TGAACTTCAA 
ACATTGCAGT CTGAACATGA TACACTGCTA GAAAGGCACA ACAAGATGCT GCAGGAAACT 
GTGTCCAAAG AGGCGGAACT CCGGGAAAAA TTGTGTTCAA TACAGTCAGA GAACATGATG 
ATGAAATCTG AACATACACA GACTGTGAGT CAGCTAACAT CCCAGAACGA GGTCCTTCGA 
AATAGCTTCC GAGATCAAGT GCGACATTTG CAGGAAGAAC ACAGAAAGAC AGTGGAGACA 
TTACAGCAGC AGCTCTCCAA GATGGAAGCA CAGCTCTTCC AGCTTAAGAA TGAACCGACC 
ACAAGAAGCC CAGTTTCCTC TCAACAATCT TTGAAGAACC TTCGAGAAAG GAGAAACACA 
GACCTCCCGC TTCTAGACAT GCACACTGTA ACCCGGGAAG AGGGAGAAGG CATGGAGACA 
ACTGATACGG AGTCTGTGTC TTCCGCCAGC ACATACACAC AGTCTTTAGA GCAGCTGCTT 
AACTCTCCCG AAACTAAACT TGAGCCTCCA TTATGGCATG CTGAATTTAC CAAAGAAGAA 
TTGGTTCAGA AGCTCAGTTC CACCACAAAA AGTGCAGATC ACTTAAACGG CCTGCTTCGG 
GAAACAGAAG CAACCAATGC AATTCTTATG GAGCAAATTA AGCTTCTCAA AAGTGAAATA 
AGAAGATTGG AAAGGAATCA AGAGCGAGAG AAGTCTGCAG CTAACCTGGA ATACTTGAAG 
AACGTCTTGC TGCAGTTCAT TTTCTTGAAA CCAGGTAGTG AAAGAGAGAG ACTTCTTCCT 
GTTATAAATA CGATGTTGCA GCTCAGCCCT GAAGAAAAGG GAAAACTTGC TGCGGTTGCT 
CAAGGTGAGG AAGAAAATGC TTCCCGTTCT TCTGGATGGG CATCCTATCT TCATAGTTGG 
TCTGGACTTC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCC2 ( XM_006712870.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712933.1 Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X1, mRNA.

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CloneID	OHu36853
Clone ID Related Accession (Same CDS sequence)	XM_006712870.3
Accession Version	XM_006712870.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	GRIP and coiled-coil domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712870.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTAA CTCAGGATCT TGTTCAAGAT GGGGTGGCTT CACCAGCTAC CCCTGGGACC 
GGGAAATCTA AGCTGGAAAC ATTGCCCAAA GAAGACCTCA TCAAGTTTGC CAAGAAACAG 
ATGATGCTAA TACAGAAAGC TAAATCAAGG TGTACAGAAT TGGAGAAAGA AATTGAAGAA 
CTCAGATCAA AACCTGTTAC TGAAGGAACT GGTGATATTA TTAAGGCATT AACTGAACGT 
CTGGATGCTC TTCTTCTGGA AAAAGCAGAG ACTGAGCAAC AGTGTCTTTC TCTGAAAAAG 
GAAAATATAA AAATGAAGCA AGAGGTTGAG GATTCTGTAA CAAAGATGGG AGATGCACAT 
AAGGAGTTGG AACAATCACA TATAAACTAT GTGAAAGAAA TTGAAAATTT GAAAAATGAG 
TTGATGGCAG TACGTTCCAA ATACAGTGAA GACAAAGCTA ACTTACAAAA GCAGCTGGAA 
GAAGCAATGA ATACGCAATT AGAACTTTCA GAACAACTTA AATTTCAGAA CAACTCTGAA 
GATAATGTTA AAAAACTACA AGAAGAGATT GAGAAAATTA GGCCAGGCTT TGAGGAGCAA 
ATTTTATATC TGCAAAAGCA ATTAGACGCT ACCACTGATG AAAAGAAGGA AACAGTTACT 
CAACTCCAAA ATATCATTGA GGCTAATTCT CAGCATTACC AAAAAAATAT TAATAGTTTG 
CAGGAAGAGC TTTTACAGTT GAAAGCTATA CACCAAGAAG AGGTGAAAGA GTTGATGTGC 
CAGATTGAAG CATCAGCTAA GGAACATGAA GCAGAGATAA ATAAGTTGAA CGAGCTAAAA 
GAGAACTTAG TAAAACAATG TGAGGCAAGT GAAAAGAACA TCCAGAAGAA ATATGAATGT 
GAGTTAGAAA ATTTAAGGAA AGCCACCTCA AATGCAAACC AAGACAATCA GATATGTTCT 
ATTCTCTTGC AAGAAAATAC ATTTGTAGAA CAAGTAGTAA ATGAAAAAGT CAAACACTTA 
GAAGATACCT TAAAAGAACT TGAATCTCAA CACAGTATCT TAAAAGATGA GGTAACTTAT 
ATGAATAATC TTAAGTTAAA ACTTGAAATG GATGCTCAAC ATATAAAGGA TGAGTTTTTT 
CATGAACGGG AAGACTTAGA GTTTAAAATT AATGAATTAT TACTAGCTAA AGAAGAACAG 
GGCTGTGTAA TTGAAAAATT AAAATCTGAG CTAGCAGGTT TAAATAAACA GTTTTGCTAT 
ACTGTAGAAC AGCATAACAG AGAAGTACAG AGTCTTAAGG AACAACATCA AAAAGAAATA 
TCAGAACTAA ATGAGACATT TTTGTCAGAT TCAGAAAAAG AAAAATTAAC ATTAATGTTT 
GAAATACAGG GTCTTAAGGA ACAGTGTGAA AACCTACAGC AAGAAAAGCA AGAAGCAATT 
TTAAATTATG AGAGTTTACG AGAGATTATG GAAATTTTAC AAACAGAACT GGGGGAATCT 
GCTGGAAAAA TAAGTCAAGA GTTCGAATCA ATGAAGCAAC AGCAAGCATC TGATGTTCAT 
GAACTGCAGC AGAAGCTCAG AACTGCTTTT ACTGAAAAAG ATGCCCTTCT CGAAACTGTG 
AATCGCCTCC AGGGAGAAAA TGAAAAGTTA CTATCTCAAC AAGAATTGGT ACCAGAACTT 
GAAAATACCA TAAAGAACCT TCAAGAAAAG AATGGAGTAT ACTTACTTAG TCTCAGTCAA 
AGAGATACCA TGTTAAAAGA ATTAGAAGGA AAGATAAATT CTCTTACTGA GGAAAAAGAT 
GATTTTATAA ATAAACTGAA AAATTCCCAT GAAGAAATGG ATAATTTCCA TAAGAAATGT 
GAAAGGGAAG AAAGATTGAT TCTTGAACTT GGGAAGAAAG TAGAGCAAAC AATCCAGTAC 
AACAGTGAAC TAGAACAAAA GGTAAATGAA TTAACAGGAG GACTAGAGGA GACTTTAAAA 
GAAAAGGATC AAAATGACCA AAAACTAGAA AAACTTATGG TTCAAATGAA AGTTCTCTCT 
GAAGACAAAG AAGTATTGTC AGCTGAAGTG AAGTCTCTTT ATGAGGAAAA CAATAAACTC 
AGTTCAGAAA AAAAACAGTT GAGTAGGGAT TTGGAGGTTT TTTTGTCTCA AAAAGAAGAT 
GTTATCCTTA AAGAACATAT TACTCAATTA GAAAAGAAAC TTCAGTTAAT GGTTGAAGAG 
CAAGATAATT TAAATAAACT GCTTGAAAAT GAGCAAGTTC AGAAGTTATT TGTTAAAACT 
CAGTTGTATG GTTTTCTTAA AGAAATGGGA TCAGAAGTTT CAGAAGACAG TGAAGAGAAA 
GATGTTGTTA ATGTCCTACA GGCAGTCGGT GAATCCTTGG CAAAAATAAA TGAGGAAAAA 
TGCAACCTGG CTTTTCAGCG TGATGAAAAA GTATTAGAGT TAGAAAAAGA GATTAAGTGC 
CTTCAAGAAG AGAGTGTAGT TCAGTGTGAA GAACTTAAGT CTTTATTGAG AGACTATGAG 
CAAGAGAAAG TTCTCTTAAG GAAAGAGTTA GAAGAAATAC AGTCAGAAAA AGAGGCCCTG 
CAGTCTGATC TTCTAGAAAT GAAGAATGCT AATGAAAAAA CAAGGCTTGA AAATCAGAAT 
CTTTTAATTC AAGTTGAAGA AGTATCTCAA ACATGTAGCA AAAGTGAAAT CCATAATGAA 
AAAGAAAAAT GTTTTATAAA GGAACATGAA AACCTAAAGC CACTACTAGA ACAAAAAGAA 
TTACGAGATA GGAGAGCAGA GTTGATACTA TTAAAGGATT CCTTAGCAAA ATCACCTTCT 
GTAAAAAATG ATCCTCTGTC TTCAGTAAAA GAGTTGGAAG AAAAAATAGA AAATCTGGAA 
AAAGAATGCA AAGAAAAGGA GGAGAAAATA AATAAGATAA AATTAGTTGC CGTAAAGGCA 
AAGAAAGAAC TAGATTCCAG CAGAAAAGAG ACCCAGACTG TGAAGGAAGA ACTTGAATCT 
CTTCGATCAG AAAAGGACCA GTTATCTGCT TCCATGAGAG ATCTCATTCA AGGAGCAGAA 
AGCTATAAGA ATCTTTTATT AGAATATGAA AAGCAGTCAG AGCAACTGGA TGTGGAAAAA 
GAACGTGCTA ATAATTTTGA GCATCGTATT GAAGACCTTA CAAGACAATT AAGAAATTCG 
ACTTTGCAGT GTGAAACAAT AAATTCTGAT AATGAAGATC TCCTGGCTCG TATTGAGACA 
TTACAGTCTA ATGCCAAATT ATTAGAAGTA CAGATTTTAG AAGTCCAGAG AGCCAAAGCA 
ATGGTAGACA AAGAATTAGA AGCTGAAAAA CTTCAGAAAG AACAGAAGAT AAAGGAACAT 
GCCACTACTG TAAATGAACT TGAAGAACTT CAGGTACAAC TTCAAAAGCA AAAGAAACAG 
CTTCAGAAAA CCATGCAAGA ATTAGAGCTG GTTAAAAAGG ATGCCCAACA AACCACATTG 
ATGAATATGG AAATAGCTGA TTATGAACGT TTGATGAAAG AACTAAATCA AAAGTTAACT 
AATAAAAACA ACAAGATAGA AGATTTGGAG CAAGAAATAA AAATTCAAAA ACAGAAACAA 
GAAACCCTAC AAGAAGAAAT AACTTCATTA CAGTCTTCAG TACAACAATA TGAAGAAAAA 
AACACCAAAA TCAAGCAATT GCTTGTGAAA ACCAAAAAGG AACTGGCAGA TTCAAAGCAA 
GCAGAAACTG ATCACTTAAT ACTTCAAGCA TCTTTAAAAG GTGAGCTGGA GGCAAGCCAG 
CAGCAAGTAG AAGTCTATAA AATACAGCTG GCTGAAATAA CATCAGAGAA GCACAAAATC 
CACGAGCACC TGAAAACCTC TGCGGAACAG CACCAGCGTA CGCTAAGTGC ATACCAGCAG 
AGAGTGACAG CACTACAGGA AGAGTGCCGT GCTGCCAAGG CAGAACAAGC TACTGTAACC 
TCTGAATTCG AGAGCTACAA AGTCCGAGTT CATAATGTTC TAAAACAACA GAAAAATAAA 
TCTATGTCTC AGGCTGAAAC TGAGGGCGCT AAACAAGAAA GGGAACATCT GGAAATGCTG 
ATTGACCAGC TAAAAATCAA ATTACAAGAT AGCCAAAATA ACTTACAGAT TAATGTATCT 
GAACTTCAAA CATTGCAGTC TGAACATGAT ACACTGCTAG AAAGGCACAA CAAGATGCTG 
CAGGAAACTG TGTCCAAAGA GGCGGAACTC CGGGAAAAAT TGTGTTCAAT ACAGTCAGAG 
AACATGATGA TGAAATCTGA ACATACACAG ACTGTGAGTC AGCTAACATC CCAGAACGAG 
GTCCTTCGAA ATAGCTTCCG AGATCAAGTG CGACATTTGC AGGAAGAACA CAGAAAGACA 
GTGGAGACAT TACAGCAGCA GCTCTCCAAG ATGGAAGCAC AGCTCTTCCA GCTTAAGAAT 
GAACCGACCA CAAGAAGCCC AGTTTCCTCT CAACAATCTT TGAAGAACCT TCGAGAAAGG 
AGAAACACAG ACCTCCCGCT TCTAGACATG CACACTGTAA CCCGGGAAGA GGGAGAAGGC 
ATGGAGACAA CTGATACGGA GTCTGTGTCT TCCGCCAGCA CATACACACA GTCTTTAGAG 
CAGCTGCTTA ACTCTCCCGA AACTAAACTT GAGCCTCCAT TATGGCATGC TGAATTTACC 
AAAGAAGAAT TGGTTCAGAA GCTCAGTTCC ACCACAAAAA GTGCAGATCA CTTAAACGGC 
CTGCTTCGGG AAACAGAAGC AACCAATGCA ATTCTTATGG AGCAAATTAA GCTTCTCAAA 
AGTGAAATAA GAAGATTGGA AAGGAATCAA GAGCGAGAGA AGTCTGCAGC TAACCTGGAA 
TACTTGAAGA ACGTCTTGCT GCAGTTCATT TTCTTGAAAC CAGGTAGTGA AAGAGAGAGA 
CTTCTTCCTG TTATAAATAC GATGTTGCAG CTCAGCCCTG AAGAAAAGGG AAAACTTGCT 
GCGGTTGCTC AAGGTGAGGA AGAAAATGCT TCCCGTTCTT CTGGATGGGC ATCCTATCTT 
CATAGTTGGT CTGGACTTCG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712933.1
CDS	715..5778
Translation	MEVTQDLVQDGVASPATPGTGKSKLETLPKEDLIKFAKKQMMLIQKAKSRCTELEKEIEELRSKPVTEGTGDIIKALTERLDALLLEKAETEQQCLSLKKENIKMKQEVEDSVTKMGDAHKELEQSHINYVKEIENLKNELMAVRSKYSEDKANLQKQLEEAMNTQLELSEQLKFQNNSEDNVKKLQEEIEKIRPGFEEQILYLQKQLDATTDEKKETVTQLQNIIEANSQHYQKNINSLQEELLQLKAIHQEEVKELMCQIEASAKEHEAEINKLNELKENLVKQCEASEKNIQKKYECELENLRKATSNANQDNQICSILLQENTFVEQVVNEKVKHLEDTLKELESQHSILKDEVTYMNNLKLKLEMDAQHIKDEFFHEREDLEFKINELLLAKEEQGCVIEKLKSELAGLNKQFCYTVEQHNREVQSLKEQHQKEISELNETFLSDSEKEKLTLMFEIQGLKEQCENLQQEKQEAILNYESLREIMEILQTELGESAGKISQEFESMKQQQASDVHELQQKLRTAFTEKDALLETVNRLQGENEKLLSQQELVPELENTIKNLQEKNGVYLLSLSQRDTMLKELEGKINSLTEEKDDFINKLKNSHEEMDNFHKKCEREERLILELGKKVEQTIQYNSELEQKVNELTGGLEETLKEKDQNDQKLEKLMVQMKVLSEDKEVLSAEVKSLYEENNKLSSEKKQLSRDLEVFLSQKEDVILKEHITQLEKKLQLMVEEQDNLNKLLENEQVQKLFVKTQLYGFLKEMGSEVSEDSEEKDVVNVLQAVGESLAKINEEKCNLAFQRDEKVLELEKEIKCLQEESVVQCEELKSLLRDYEQEKVLLRKELEEIQSEKEALQSDLLEMKNANEKTRLENQNLLIQVEEVSQTCSKSEIHNEKEKCFIKEHENLKPLLEQKELRDRRAELILLKDSLAKSPSVKNDPLSSVKELEEKIENLEKECKEKEEKINKIKLVAVKAKKELDSSRKETQTVKEELESLRSEKDQLSASMRDLIQGAESYKNLLLEYEKQSEQLDVEKERANNFEHRIEDLTRQLRNSTLQCETINSDNEDLLARIETLQSNAKLLEVQILEVQRAKAMVDKELEAEKLQKEQKIKEHATTVNELEELQVQLQKQKKQLQKTMQELELVKKDAQQTTLMNMEIADYERLMKELNQKLTNKNNKIEDLEQEIKIQKQKQETLQEEITSLQSSVQQYEEKNTKIKQLLVKTKKELADSKQAETDHLILQASLKGELEASQQQVEVYKIQLAEITSEKHKIHEHLKTSAEQHQRTLSAYQQRVTALQEECRAAKAEQATVTSEFESYKVRVHNVLKQQKNKSMSQAETEGAKQEREHLEMLIDQLKIKLQDSQNNLQINVSELQTLQSEHDTLLERHNKMLQETVSKEAELREKLCSIQSENMMMKSEHTQTVSQLTSQNEVLRNSFRDQVRHLQEEHRKTVETLQQQLSKMEAQLFQLKNEPTTRSPVSSQQSLKNLRERRNTDLPLLDMHTVTREEGEGMETTDTESVSSASTYTQSLEQLLNSPETKLEPPLWHAEFTKEELVQKLSSTTKSADHLNGLLRETEATNAILMEQIKLLKSEIRRLERNQEREKSAANLEYLKNVLLQFIFLKPGSERERLLPVINTMLQLSPEEKGKLAAVAQGEEENASRSSGWASYLHSWSGLR

Target ORF information:

RefSeq Version	XM_006712870.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712870.3

ORF Insert Sequence:

ATGGAGGTAA CTCAGGATCT TGTTCAAGAT GGGGTGGCTT CACCAGCTAC CCCTGGGACC 
GGGAAATCTA AGCTGGAAAC ATTGCCCAAA GAAGACCTCA TCAAGTTTGC CAAGAAACAG 
ATGATGCTAA TACAGAAAGC TAAATCAAGG TGTACAGAAT TGGAGAAAGA AATTGAAGAA 
CTCAGATCAA AACCTGTTAC TGAAGGAACT GGTGATATTA TTAAGGCATT AACTGAACGT 
CTGGATGCTC TTCTTCTGGA AAAAGCAGAG ACTGAGCAAC AGTGTCTTTC TCTGAAAAAG 
GAAAATATAA AAATGAAGCA AGAGGTTGAG GATTCTGTAA CAAAGATGGG AGATGCACAT 
AAGGAGTTGG AACAATCACA TATAAACTAT GTGAAAGAAA TTGAAAATTT GAAAAATGAG 
TTGATGGCAG TACGTTCCAA ATACAGTGAA GACAAAGCTA ACTTACAAAA GCAGCTGGAA 
GAAGCAATGA ATACGCAATT AGAACTTTCA GAACAACTTA AATTTCAGAA CAACTCTGAA 
GATAATGTTA AAAAACTACA AGAAGAGATT GAGAAAATTA GGCCAGGCTT TGAGGAGCAA 
ATTTTATATC TGCAAAAGCA ATTAGACGCT ACCACTGATG AAAAGAAGGA AACAGTTACT 
CAACTCCAAA ATATCATTGA GGCTAATTCT CAGCATTACC AAAAAAATAT TAATAGTTTG 
CAGGAAGAGC TTTTACAGTT GAAAGCTATA CACCAAGAAG AGGTGAAAGA GTTGATGTGC 
CAGATTGAAG CATCAGCTAA GGAACATGAA GCAGAGATAA ATAAGTTGAA CGAGCTAAAA 
GAGAACTTAG TAAAACAATG TGAGGCAAGT GAAAAGAACA TCCAGAAGAA ATATGAATGT 
GAGTTAGAAA ATTTAAGGAA AGCCACCTCA AATGCAAACC AAGACAATCA GATATGTTCT 
ATTCTCTTGC AAGAAAATAC ATTTGTAGAA CAAGTAGTAA ATGAAAAAGT CAAACACTTA 
GAAGATACCT TAAAAGAACT TGAATCTCAA CACAGTATCT TAAAAGATGA GGTAACTTAT 
ATGAATAATC TTAAGTTAAA ACTTGAAATG GATGCTCAAC ATATAAAGGA TGAGTTTTTT 
CATGAACGGG AAGACTTAGA GTTTAAAATT AATGAATTAT TACTAGCTAA AGAAGAACAG 
GGCTGTGTAA TTGAAAAATT AAAATCTGAG CTAGCAGGTT TAAATAAACA GTTTTGCTAT 
ACTGTAGAAC AGCATAACAG AGAAGTACAG AGTCTTAAGG AACAACATCA AAAAGAAATA 
TCAGAACTAA ATGAGACATT TTTGTCAGAT TCAGAAAAAG AAAAATTAAC ATTAATGTTT 
GAAATACAGG GTCTTAAGGA ACAGTGTGAA AACCTACAGC AAGAAAAGCA AGAAGCAATT 
TTAAATTATG AGAGTTTACG AGAGATTATG GAAATTTTAC AAACAGAACT GGGGGAATCT 
GCTGGAAAAA TAAGTCAAGA GTTCGAATCA ATGAAGCAAC AGCAAGCATC TGATGTTCAT 
GAACTGCAGC AGAAGCTCAG AACTGCTTTT ACTGAAAAAG ATGCCCTTCT CGAAACTGTG 
AATCGCCTCC AGGGAGAAAA TGAAAAGTTA CTATCTCAAC AAGAATTGGT ACCAGAACTT 
GAAAATACCA TAAAGAACCT TCAAGAAAAG AATGGAGTAT ACTTACTTAG TCTCAGTCAA 
AGAGATACCA TGTTAAAAGA ATTAGAAGGA AAGATAAATT CTCTTACTGA GGAAAAAGAT 
GATTTTATAA ATAAACTGAA AAATTCCCAT GAAGAAATGG ATAATTTCCA TAAGAAATGT 
GAAAGGGAAG AAAGATTGAT TCTTGAACTT GGGAAGAAAG TAGAGCAAAC AATCCAGTAC 
AACAGTGAAC TAGAACAAAA GGTAAATGAA TTAACAGGAG GACTAGAGGA GACTTTAAAA 
GAAAAGGATC AAAATGACCA AAAACTAGAA AAACTTATGG TTCAAATGAA AGTTCTCTCT 
GAAGACAAAG AAGTATTGTC AGCTGAAGTG AAGTCTCTTT ATGAGGAAAA CAATAAACTC 
AGTTCAGAAA AAAAACAGTT GAGTAGGGAT TTGGAGGTTT TTTTGTCTCA AAAAGAAGAT 
GTTATCCTTA AAGAACATAT TACTCAATTA GAAAAGAAAC TTCAGTTAAT GGTTGAAGAG 
CAAGATAATT TAAATAAACT GCTTGAAAAT GAGCAAGTTC AGAAGTTATT TGTTAAAACT 
CAGTTGTATG GTTTTCTTAA AGAAATGGGA TCAGAAGTTT CAGAAGACAG TGAAGAGAAA 
GATGTTGTTA ATGTCCTACA GGCAGTCGGT GAATCCTTGG CAAAAATAAA TGAGGAAAAA 
TGCAACCTGG CTTTTCAGCG TGATGAAAAA GTATTAGAGT TAGAAAAAGA GATTAAGTGC 
CTTCAAGAAG AGAGTGTAGT TCAGTGTGAA GAACTTAAGT CTTTATTGAG AGACTATGAG 
CAAGAGAAAG TTCTCTTAAG GAAAGAGTTA GAAGAAATAC AGTCAGAAAA AGAGGCCCTG 
CAGTCTGATC TTCTAGAAAT GAAGAATGCT AATGAAAAAA CAAGGCTTGA AAATCAGAAT 
CTTTTAATTC AAGTTGAAGA AGTATCTCAA ACATGTAGCA AAAGTGAAAT CCATAATGAA 
AAAGAAAAAT GTTTTATAAA GGAACATGAA AACCTAAAGC CACTACTAGA ACAAAAAGAA 
TTACGAGATA GGAGAGCAGA GTTGATACTA TTAAAGGATT CCTTAGCAAA ATCACCTTCT 
GTAAAAAATG ATCCTCTGTC TTCAGTAAAA GAGTTGGAAG AAAAAATAGA AAATCTGGAA 
AAAGAATGCA AAGAAAAGGA GGAGAAAATA AATAAGATAA AATTAGTTGC CGTAAAGGCA 
AAGAAAGAAC TAGATTCCAG CAGAAAAGAG ACCCAGACTG TGAAGGAAGA ACTTGAATCT 
CTTCGATCAG AAAAGGACCA GTTATCTGCT TCCATGAGAG ATCTCATTCA AGGAGCAGAA 
AGCTATAAGA ATCTTTTATT AGAATATGAA AAGCAGTCAG AGCAACTGGA TGTGGAAAAA 
GAACGTGCTA ATAATTTTGA GCATCGTATT GAAGACCTTA CAAGACAATT AAGAAATTCG 
ACTTTGCAGT GTGAAACAAT AAATTCTGAT AATGAAGATC TCCTGGCTCG TATTGAGACA 
TTACAGTCTA ATGCCAAATT ATTAGAAGTA CAGATTTTAG AAGTCCAGAG AGCCAAAGCA 
ATGGTAGACA AAGAATTAGA AGCTGAAAAA CTTCAGAAAG AACAGAAGAT AAAGGAACAT 
GCCACTACTG TAAATGAACT TGAAGAACTT CAGGTACAAC TTCAAAAGCA AAAGAAACAG 
CTTCAGAAAA CCATGCAAGA ATTAGAGCTG GTTAAAAAGG ATGCCCAACA AACCACATTG 
ATGAATATGG AAATAGCTGA TTATGAACGT TTGATGAAAG AACTAAATCA AAAGTTAACT 
AATAAAAACA ACAAGATAGA AGATTTGGAG CAAGAAATAA AAATTCAAAA ACAGAAACAA 
GAAACCCTAC AAGAAGAAAT AACTTCATTA CAGTCTTCAG TACAACAATA TGAAGAAAAA 
AACACCAAAA TCAAGCAATT GCTTGTGAAA ACCAAAAAGG AACTGGCAGA TTCAAAGCAA 
GCAGAAACTG ATCACTTAAT ACTTCAAGCA TCTTTAAAAG GTGAGCTGGA GGCAAGCCAG 
CAGCAAGTAG AAGTCTATAA AATACAGCTG GCTGAAATAA CATCAGAGAA GCACAAAATC 
CACGAGCACC TGAAAACCTC TGCGGAACAG CACCAGCGTA CGCTAAGTGC ATACCAGCAG 
AGAGTGACAG CACTACAGGA AGAGTGCCGT GCTGCCAAGG CAGAACAAGC TACTGTAACC 
TCTGAATTCG AGAGCTACAA AGTCCGAGTT CATAATGTTC TAAAACAACA GAAAAATAAA 
TCTATGTCTC AGGCTGAAAC TGAGGGCGCT AAACAAGAAA GGGAACATCT GGAAATGCTG 
ATTGACCAGC TAAAAATCAA ATTACAAGAT AGCCAAAATA ACTTACAGAT TAATGTATCT 
GAACTTCAAA CATTGCAGTC TGAACATGAT ACACTGCTAG AAAGGCACAA CAAGATGCTG 
CAGGAAACTG TGTCCAAAGA GGCGGAACTC CGGGAAAAAT TGTGTTCAAT ACAGTCAGAG 
AACATGATGA TGAAATCTGA ACATACACAG ACTGTGAGTC AGCTAACATC CCAGAACGAG 
GTCCTTCGAA ATAGCTTCCG AGATCAAGTG CGACATTTGC AGGAAGAACA CAGAAAGACA 
GTGGAGACAT TACAGCAGCA GCTCTCCAAG ATGGAAGCAC AGCTCTTCCA GCTTAAGAAT 
GAACCGACCA CAAGAAGCCC AGTTTCCTCT CAACAATCTT TGAAGAACCT TCGAGAAAGG 
AGAAACACAG ACCTCCCGCT TCTAGACATG CACACTGTAA CCCGGGAAGA GGGAGAAGGC 
ATGGAGACAA CTGATACGGA GTCTGTGTCT TCCGCCAGCA CATACACACA GTCTTTAGAG 
CAGCTGCTTA ACTCTCCCGA AACTAAACTT GAGCCTCCAT TATGGCATGC TGAATTTACC 
AAAGAAGAAT TGGTTCAGAA GCTCAGTTCC ACCACAAAAA GTGCAGATCA CTTAAACGGC 
CTGCTTCGGG AAACAGAAGC AACCAATGCA ATTCTTATGG AGCAAATTAA GCTTCTCAAA 
AGTGAAATAA GAAGATTGGA AAGGAATCAA GAGCGAGAGA AGTCTGCAGC TAACCTGGAA 
TACTTGAAGA ACGTCTTGCT GCAGTTCATT TTCTTGAAAC CAGGTAGTGA AAGAGAGAGA 
CTTCTTCCTG TTATAAATAC GATGTTGCAG CTCAGCCCTG AAGAAAAGGG AAAACTTGCT 
GCGGTTGCTC AAGGTGAGGA AGAAAATGCT TCCCGTTCTT CTGGATGGGC ATCCTATCTT 
CATAGTTGGT CTGGACTTCG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCC2 ( XM_006712871.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712934.1 Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X2, mRNA.

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CloneID	OHu36854
Clone ID Related Accession (Same CDS sequence)	XM_006712871.1
Accession Version	XM_006712871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	GRIP and coiled-coil domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCAAG AGGTTGAGGA TTCTGTAACA AAGATGGGAG ATGCACATAA GGAGTTGGAA 
CAATCACATA TAAACTATGT GAAAGAAATT GAAAATTTGA AAAATGAGTT GATGGCAGTA 
CGTTCCAAAT ACAGTGAAGA CAAAGCTAAC TTACAAAAGC AGCTGGAAGA AGCAATGAAT 
ACGCAATTAG AACTTTCAGA ACAACTTAAA TTTCAGAACA ACTCTGAAGA TAATGTTAAA 
AAACTACAAG AAGAGATTGA GAAAATTAGG CCAGGCTTTG AGGAGCAAAT TTTATATCTG 
CAAAAGCAAT TAGACGCTAC CACTGATGAA AAGAAGGAAA CAGTTACTCA ACTCCAAAAT 
ATCATTGAGG CTAATTCTCA GCATTACCAA AAAAATATTA ATAGTTTGCA GGAAGAGCTT 
TTACAGTTGA AAGCTATACA CCAAGAAGAG GTGAAAGAGT TGATGTGCCA GATTGAAGCA 
TCAGCTAAGG AACATGAAGC AGAGATAAAT AAGTTGAACG AGCTAAAAGA GAACTTAGTA 
AAACAATGTG AGGCAAGTGA AAAGAACATC CAGAAGAAAT ATGAATGTGA GTTAGAAAAT 
TTAAGGAAAG CCACCTCAAA TGCAAACCAA GACAATCAGA TATGTTCTAT TCTCTTGCAA 
GAAAATACAT TTGTAGAACA AGTAGTAAAT GAAAAAGTCA AACACTTAGA AGATACCTTA 
AAAGAACTTG AATCTCAACA CAGTATCTTA AAAGATGAGG TAACTTATAT GAATAATCTT 
AAGTTAAAAC TTGAAATGGA TGCTCAACAT ATAAAGGATG AGTTTTTTCA TGAACGGGAA 
GACTTAGAGT TTAAAATTAA TGAATTATTA CTAGCTAAAG AAGAACAGGG CTGTGTAATT 
GAAAAATTAA AATCTGAGCT AGCAGGTTTA AATAAACAGT TTTGCTATAC TGTAGAACAG 
CATAACAGAG AAGTACAGAG TCTTAAGGAA CAACATCAAA AAGAAATATC AGAACTAAAT 
GAGACATTTT TGTCAGATTC AGAAAAAGAA AAATTAACAT TAATGTTTGA AATACAGGGT 
CTTAAGGAAC AGTGTGAAAA CCTACAGCAA GAAAAGCAAG AAGCAATTTT AAATTATGAG 
AGTTTACGAG AGATTATGGA AATTTTACAA ACAGAACTGG GGGAATCTGC TGGAAAAATA 
AGTCAAGAGT TCGAATCAAT GAAGCAACAG CAAGCATCTG ATGTTCATGA ACTGCAGCAG 
AAGCTCAGAA CTGCTTTTAC TGAAAAAGAT GCCCTTCTCG AAACTGTGAA TCGCCTCCAG 
GGAGAAAATG AAAAGTTACT ATCTCAACAA GAATTGGTAC CAGAACTTGA AAATACCATA 
AAGAACCTTC AAGAAAAGAA TGGAGTATAC TTACTTAGTC TCAGTCAAAG AGATACCATG 
TTAAAAGAAT TAGAAGGAAA GATAAATTCT CTTACTGAGG AAAAAGATGA TTTTATAAAT 
AAACTGAAAA ATTCCCATGA AGAAATGGAT AATTTCCATA AGAAATGTGA AAGGGAAGAA 
AGATTGATTC TTGAACTTGG GAAGAAAGTA GAGCAAACAA TCCAGTACAA CAGTGAACTA 
GAACAAAAGG TAAATGAATT AACAGGAGGA CTAGAGGAGA CTTTAAAAGA AAAGGATCAA 
AATGACCAAA AACTAGAAAA ACTTATGGTT CAAATGAAAG TTCTCTCTGA AGACAAAGAA 
GTATTGTCAG CTGAAGTGAA GTCTCTTTAT GAGGAAAACA ATAAACTCAG TTCAGAAAAA 
AAACAGTTGA GTAGGGATTT GGAGGTTTTT TTGTCTCAAA AAGAAGATGT TATCCTTAAA 
GAACATATTA CTCAATTAGA AAAGAAACTT CAGTTAATGG TTGAAGAGCA AGATAATTTA 
AATAAACTGC TTGAAAATGA GCAAGTTCAG AAGTTATTTG TTAAAACTCA GTTGTATGGT 
TTTCTTAAAG AAATGGGATC AGAAGTTTCA GAAGACAGTG AAGAGAAAGA TGTTGTTAAT 
GTCCTACAGG CAGTCGGTGA ATCCTTGGCA AAAATAAATG AGGAAAAATG CAACCTGGCT 
TTTCAGCGTG ATGAAAAAGT ATTAGAGTTA GAAAAAGAGA TTAAGTGCCT TCAAGAAGAG 
AGTGTAGTTC AGTGTGAAGA ACTTAAGTCT TTATTGAGAG ACTATGAGCA AGAGAAAGTT 
CTCTTAAGGA AAGAGTTAGA AGAAATACAG TCAGAAAAAG AGGCCCTGCA GTCTGATCTT 
CTAGAAATGA AGAATGCTAA TGAAAAAACA AGGCTTGAAA ATCAGAATCT TTTAATTCAA 
GTTGAAGAAG TATCTCAAAC ATGTAGCAAA AGTGAAATCC ATAATGAAAA AGAAAAATGT 
TTTATAAAGG AACATGAAAA CCTAAAGCCA CTACTAGAAC AAAAAGAATT ACGAGATAGG 
AGAGCAGAGT TGATACTATT AAAGGATTCC TTAGCAAAAT CACCTTCTGT AAAAAATGAT 
CCTCTGTCTT CAGTAAAAGA GTTGGAAGAA AAAATAGAAA ATCTGGAAAA AGAATGCAAA 
GAAAAGGAGG AGAAAATAAA TAAGATAAAA TTAGTTGCCG TAAAGGCAAA GAAAGAACTA 
GATTCCAGCA GAAAAGAGAC CCAGACTGTG AAGGAAGAAC TTGAATCTCT TCGATCAGAA 
AAGGACCAGT TATCTGCTTC CATGAGAGAT CTCATTCAAG GAGCAGAAAG CTATAAGAAT 
CTTTTATTAG AATATGAAAA GCAGTCAGAG CAACTGGATG TGGAAAAAGA ACGTGCTAAT 
AATTTTGAGC ATCGTATTGA AGACCTTACA AGACAATTAA GAAATTCGAC TTTGCAGTGT 
GAAACAATAA ATTCTGATAA TGAAGATCTC CTGGCTCGTA TTGAGACATT ACAGTCTAAT 
GCCAAATTAT TAGAAGTACA GATTTTAGAA GTCCAGAGAG CCAAAGCAAT GGTAGACAAA 
GAATTAGAAG CTGAAAAACT TCAGAAAGAA CAGAAGATAA AGGAACATGC CACTACTGTA 
AATGAACTTG AAGAACTTCA GGTACAACTT CAAAAGCAAA AGAAACAGCT TCAGAAAACC 
ATGCAAGAAT TAGAGCTGGT TAAAAAGGAT GCCCAACAAA CCACATTGAT GAATATGGAA 
ATAGCTGATT ATGAACGTTT GATGAAAGAA CTAAATCAAA AGTTAACTAA TAAAAACAAC 
AAGATAGAAG ATTTGGAGCA AGAAATAAAA ATTCAAAAAC AGAAACAAGA AACCCTACAA 
GAAGAAATAA CTTCATTACA GTCTTCAGTA CAACAATATG AAGAAAAAAA CACCAAAATC 
AAGCAATTGC TTGTGAAAAC CAAAAAGGAA CTGGCAGATT CAAAGCAAGC AGAAACTGAT 
CACTTAATAC TTCAAGCATC TTTAAAAGGT GAGCTGGAGG CAAGCCAGCA GCAAGTAGAA 
GTCTATAAAA TACAGCTGGC TGAAATAACA TCAGAGAAGC ACAAAATCCA CGAGCACCTG 
AAAACCTCTG CGGAACAGCA CCAGCGTACG CTAAGTGCAT ACCAGCAGAG AGTGACAGCA 
CTACAGGAAG AGTGCCGTGC TGCCAAGGCA GAACAAGCTA CTGTAACCTC TGAATTCGAG 
AGCTACAAAG TCCGAGTTCA TAATGTTCTA AAACAACAGA AAAATAAATC TATGTCTCAG 
GCTGAAACTG AGGGCGCTAA ACAAGAAAGG GAACATCTGG AAATGCTGAT TGACCAGCTA 
AAAATCAAAT TACAAGATAG CCAAAATAAC TTACAGATTA ATGTATCTGA ACTTCAAACA 
TTGCAGTCTG AACATGATAC ACTGCTAGAA AGGCACAACA AGATGCTGCA GGAAACTGTG 
TCCAAAGAGG CGGAACTCCG GGAAAAATTG TGTTCAATAC AGTCAGAGAA CATGATGATG 
AAATCTGAAC ATACACAGAC TGTGAGTCAG CTAACATCCC AGAACGAGGT CCTTCGAAAT 
AGCTTCCGAG ATCAAGTGCG ACATTTGCAG GAAGAACACA GAAAGACAGT GGAGACATTA 
CAGCAGCAGC TCTCCAAGAT GGAAGCACAG CTCTTCCAGC TTAAGAATGA ACCGACCACA 
AGAAGCCCAG TTTCCTCTCA ACAATCTTTG AAGAACCTTC GAGAAAGGAG AAACACAGAC 
CTCCCGCTTC TAGACATGCA CACTGTAACC CGGGAAGAGG GAGAAGGCAT GGAGACAACT 
GATACGGAGT CTGTGTCTTC CGCCAGCACA TACACACAGT CTTTAGAGCA GCTGCTTAAC 
TCTCCCGAAA CTAAACTTGA GCCTCCATTA TGGCATGCTG AATTTACCAA AGAAGAATTG 
GTTCAGAAGC TCAGTTCCAC CACAAAAAGT GCAGATCACT TAAACGGCCT GCTTCGGGAA 
ACAGAAGCAA CCAATGCAAT TCTTATGGAG CAAATTAAGC TTCTCAAAAG TGAAATAAGA 
AGATTGGAAA GGAATCAAGA GCGAGAGAAG TCTGCAGCTA ACCTGGAATA CTTGAAGAAC 
GTCTTGCTGC AGTTCATTTT CTTGAAACCA GGTAGTGAAA GAGAGAGACT TCTTCCTGTT 
ATAAATACGA TGTTGCAGCT CAGCCCTGAA GAAAAGGGAA AACTTGCTGC GGTTGCTCAA 
GGTGAGGAAG AAAATGCTTC CCGTTCTTCT GGATGGGCAT CCTATCTTCA TAGTTGGTCT 
GGACTTCGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712934.1
CDS	281..5032
Translation	MKQEVEDSVTKMGDAHKELEQSHINYVKEIENLKNELMAVRSKYSEDKANLQKQLEEAMNTQLELSEQLKFQNNSEDNVKKLQEEIEKIRPGFEEQILYLQKQLDATTDEKKETVTQLQNIIEANSQHYQKNINSLQEELLQLKAIHQEEVKELMCQIEASAKEHEAEINKLNELKENLVKQCEASEKNIQKKYECELENLRKATSNANQDNQICSILLQENTFVEQVVNEKVKHLEDTLKELESQHSILKDEVTYMNNLKLKLEMDAQHIKDEFFHEREDLEFKINELLLAKEEQGCVIEKLKSELAGLNKQFCYTVEQHNREVQSLKEQHQKEISELNETFLSDSEKEKLTLMFEIQGLKEQCENLQQEKQEAILNYESLREIMEILQTELGESAGKISQEFESMKQQQASDVHELQQKLRTAFTEKDALLETVNRLQGENEKLLSQQELVPELENTIKNLQEKNGVYLLSLSQRDTMLKELEGKINSLTEEKDDFINKLKNSHEEMDNFHKKCEREERLILELGKKVEQTIQYNSELEQKVNELTGGLEETLKEKDQNDQKLEKLMVQMKVLSEDKEVLSAEVKSLYEENNKLSSEKKQLSRDLEVFLSQKEDVILKEHITQLEKKLQLMVEEQDNLNKLLENEQVQKLFVKTQLYGFLKEMGSEVSEDSEEKDVVNVLQAVGESLAKINEEKCNLAFQRDEKVLELEKEIKCLQEESVVQCEELKSLLRDYEQEKVLLRKELEEIQSEKEALQSDLLEMKNANEKTRLENQNLLIQVEEVSQTCSKSEIHNEKEKCFIKEHENLKPLLEQKELRDRRAELILLKDSLAKSPSVKNDPLSSVKELEEKIENLEKECKEKEEKINKIKLVAVKAKKELDSSRKETQTVKEELESLRSEKDQLSASMRDLIQGAESYKNLLLEYEKQSEQLDVEKERANNFEHRIEDLTRQLRNSTLQCETINSDNEDLLARIETLQSNAKLLEVQILEVQRAKAMVDKELEAEKLQKEQKIKEHATTVNELEELQVQLQKQKKQLQKTMQELELVKKDAQQTTLMNMEIADYERLMKELNQKLTNKNNKIEDLEQEIKIQKQKQETLQEEITSLQSSVQQYEEKNTKIKQLLVKTKKELADSKQAETDHLILQASLKGELEASQQQVEVYKIQLAEITSEKHKIHEHLKTSAEQHQRTLSAYQQRVTALQEECRAAKAEQATVTSEFESYKVRVHNVLKQQKNKSMSQAETEGAKQEREHLEMLIDQLKIKLQDSQNNLQINVSELQTLQSEHDTLLERHNKMLQETVSKEAELREKLCSIQSENMMMKSEHTQTVSQLTSQNEVLRNSFRDQVRHLQEEHRKTVETLQQQLSKMEAQLFQLKNEPTTRSPVSSQQSLKNLRERRNTDLPLLDMHTVTREEGEGMETTDTESVSSASTYTQSLEQLLNSPETKLEPPLWHAEFTKEELVQKLSSTTKSADHLNGLLRETEATNAILMEQIKLLKSEIRRLERNQEREKSAANLEYLKNVLLQFIFLKPGSERERLLPVINTMLQLSPEEKGKLAAVAQGEEENASRSSGWASYLHSWSGLR

Target ORF information:

RefSeq Version	XM_006712871.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712871.1

ORF Insert Sequence:

ATGAAGCAAG AGGTTGAGGA TTCTGTAACA AAGATGGGAG ATGCACATAA GGAGTTGGAA 
CAATCACATA TAAACTATGT GAAAGAAATT GAAAATTTGA AAAATGAGTT GATGGCAGTA 
CGTTCCAAAT ACAGTGAAGA CAAAGCTAAC TTACAAAAGC AGCTGGAAGA AGCAATGAAT 
ACGCAATTAG AACTTTCAGA ACAACTTAAA TTTCAGAACA ACTCTGAAGA TAATGTTAAA 
AAACTACAAG AAGAGATTGA GAAAATTAGG CCAGGCTTTG AGGAGCAAAT TTTATATCTG 
CAAAAGCAAT TAGACGCTAC CACTGATGAA AAGAAGGAAA CAGTTACTCA ACTCCAAAAT 
ATCATTGAGG CTAATTCTCA GCATTACCAA AAAAATATTA ATAGTTTGCA GGAAGAGCTT 
TTACAGTTGA AAGCTATACA CCAAGAAGAG GTGAAAGAGT TGATGTGCCA GATTGAAGCA 
TCAGCTAAGG AACATGAAGC AGAGATAAAT AAGTTGAACG AGCTAAAAGA GAACTTAGTA 
AAACAATGTG AGGCAAGTGA AAAGAACATC CAGAAGAAAT ATGAATGTGA GTTAGAAAAT 
TTAAGGAAAG CCACCTCAAA TGCAAACCAA GACAATCAGA TATGTTCTAT TCTCTTGCAA 
GAAAATACAT TTGTAGAACA AGTAGTAAAT GAAAAAGTCA AACACTTAGA AGATACCTTA 
AAAGAACTTG AATCTCAACA CAGTATCTTA AAAGATGAGG TAACTTATAT GAATAATCTT 
AAGTTAAAAC TTGAAATGGA TGCTCAACAT ATAAAGGATG AGTTTTTTCA TGAACGGGAA 
GACTTAGAGT TTAAAATTAA TGAATTATTA CTAGCTAAAG AAGAACAGGG CTGTGTAATT 
GAAAAATTAA AATCTGAGCT AGCAGGTTTA AATAAACAGT TTTGCTATAC TGTAGAACAG 
CATAACAGAG AAGTACAGAG TCTTAAGGAA CAACATCAAA AAGAAATATC AGAACTAAAT 
GAGACATTTT TGTCAGATTC AGAAAAAGAA AAATTAACAT TAATGTTTGA AATACAGGGT 
CTTAAGGAAC AGTGTGAAAA CCTACAGCAA GAAAAGCAAG AAGCAATTTT AAATTATGAG 
AGTTTACGAG AGATTATGGA AATTTTACAA ACAGAACTGG GGGAATCTGC TGGAAAAATA 
AGTCAAGAGT TCGAATCAAT GAAGCAACAG CAAGCATCTG ATGTTCATGA ACTGCAGCAG 
AAGCTCAGAA CTGCTTTTAC TGAAAAAGAT GCCCTTCTCG AAACTGTGAA TCGCCTCCAG 
GGAGAAAATG AAAAGTTACT ATCTCAACAA GAATTGGTAC CAGAACTTGA AAATACCATA 
AAGAACCTTC AAGAAAAGAA TGGAGTATAC TTACTTAGTC TCAGTCAAAG AGATACCATG 
TTAAAAGAAT TAGAAGGAAA GATAAATTCT CTTACTGAGG AAAAAGATGA TTTTATAAAT 
AAACTGAAAA ATTCCCATGA AGAAATGGAT AATTTCCATA AGAAATGTGA AAGGGAAGAA 
AGATTGATTC TTGAACTTGG GAAGAAAGTA GAGCAAACAA TCCAGTACAA CAGTGAACTA 
GAACAAAAGG TAAATGAATT AACAGGAGGA CTAGAGGAGA CTTTAAAAGA AAAGGATCAA 
AATGACCAAA AACTAGAAAA ACTTATGGTT CAAATGAAAG TTCTCTCTGA AGACAAAGAA 
GTATTGTCAG CTGAAGTGAA GTCTCTTTAT GAGGAAAACA ATAAACTCAG TTCAGAAAAA 
AAACAGTTGA GTAGGGATTT GGAGGTTTTT TTGTCTCAAA AAGAAGATGT TATCCTTAAA 
GAACATATTA CTCAATTAGA AAAGAAACTT CAGTTAATGG TTGAAGAGCA AGATAATTTA 
AATAAACTGC TTGAAAATGA GCAAGTTCAG AAGTTATTTG TTAAAACTCA GTTGTATGGT 
TTTCTTAAAG AAATGGGATC AGAAGTTTCA GAAGACAGTG AAGAGAAAGA TGTTGTTAAT 
GTCCTACAGG CAGTCGGTGA ATCCTTGGCA AAAATAAATG AGGAAAAATG CAACCTGGCT 
TTTCAGCGTG ATGAAAAAGT ATTAGAGTTA GAAAAAGAGA TTAAGTGCCT TCAAGAAGAG 
AGTGTAGTTC AGTGTGAAGA ACTTAAGTCT TTATTGAGAG ACTATGAGCA AGAGAAAGTT 
CTCTTAAGGA AAGAGTTAGA AGAAATACAG TCAGAAAAAG AGGCCCTGCA GTCTGATCTT 
CTAGAAATGA AGAATGCTAA TGAAAAAACA AGGCTTGAAA ATCAGAATCT TTTAATTCAA 
GTTGAAGAAG TATCTCAAAC ATGTAGCAAA AGTGAAATCC ATAATGAAAA AGAAAAATGT 
TTTATAAAGG AACATGAAAA CCTAAAGCCA CTACTAGAAC AAAAAGAATT ACGAGATAGG 
AGAGCAGAGT TGATACTATT AAAGGATTCC TTAGCAAAAT CACCTTCTGT AAAAAATGAT 
CCTCTGTCTT CAGTAAAAGA GTTGGAAGAA AAAATAGAAA ATCTGGAAAA AGAATGCAAA 
GAAAAGGAGG AGAAAATAAA TAAGATAAAA TTAGTTGCCG TAAAGGCAAA GAAAGAACTA 
GATTCCAGCA GAAAAGAGAC CCAGACTGTG AAGGAAGAAC TTGAATCTCT TCGATCAGAA 
AAGGACCAGT TATCTGCTTC CATGAGAGAT CTCATTCAAG GAGCAGAAAG CTATAAGAAT 
CTTTTATTAG AATATGAAAA GCAGTCAGAG CAACTGGATG TGGAAAAAGA ACGTGCTAAT 
AATTTTGAGC ATCGTATTGA AGACCTTACA AGACAATTAA GAAATTCGAC TTTGCAGTGT 
GAAACAATAA ATTCTGATAA TGAAGATCTC CTGGCTCGTA TTGAGACATT ACAGTCTAAT 
GCCAAATTAT TAGAAGTACA GATTTTAGAA GTCCAGAGAG CCAAAGCAAT GGTAGACAAA 
GAATTAGAAG CTGAAAAACT TCAGAAAGAA CAGAAGATAA AGGAACATGC CACTACTGTA 
AATGAACTTG AAGAACTTCA GGTACAACTT CAAAAGCAAA AGAAACAGCT TCAGAAAACC 
ATGCAAGAAT TAGAGCTGGT TAAAAAGGAT GCCCAACAAA CCACATTGAT GAATATGGAA 
ATAGCTGATT ATGAACGTTT GATGAAAGAA CTAAATCAAA AGTTAACTAA TAAAAACAAC 
AAGATAGAAG ATTTGGAGCA AGAAATAAAA ATTCAAAAAC AGAAACAAGA AACCCTACAA 
GAAGAAATAA CTTCATTACA GTCTTCAGTA CAACAATATG AAGAAAAAAA CACCAAAATC 
AAGCAATTGC TTGTGAAAAC CAAAAAGGAA CTGGCAGATT CAAAGCAAGC AGAAACTGAT 
CACTTAATAC TTCAAGCATC TTTAAAAGGT GAGCTGGAGG CAAGCCAGCA GCAAGTAGAA 
GTCTATAAAA TACAGCTGGC TGAAATAACA TCAGAGAAGC ACAAAATCCA CGAGCACCTG 
AAAACCTCTG CGGAACAGCA CCAGCGTACG CTAAGTGCAT ACCAGCAGAG AGTGACAGCA 
CTACAGGAAG AGTGCCGTGC TGCCAAGGCA GAACAAGCTA CTGTAACCTC TGAATTCGAG 
AGCTACAAAG TCCGAGTTCA TAATGTTCTA AAACAACAGA AAAATAAATC TATGTCTCAG 
GCTGAAACTG AGGGCGCTAA ACAAGAAAGG GAACATCTGG AAATGCTGAT TGACCAGCTA 
AAAATCAAAT TACAAGATAG CCAAAATAAC TTACAGATTA ATGTATCTGA ACTTCAAACA 
TTGCAGTCTG AACATGATAC ACTGCTAGAA AGGCACAACA AGATGCTGCA GGAAACTGTG 
TCCAAAGAGG CGGAACTCCG GGAAAAATTG TGTTCAATAC AGTCAGAGAA CATGATGATG 
AAATCTGAAC ATACACAGAC TGTGAGTCAG CTAACATCCC AGAACGAGGT CCTTCGAAAT 
AGCTTCCGAG ATCAAGTGCG ACATTTGCAG GAAGAACACA GAAAGACAGT GGAGACATTA 
CAGCAGCAGC TCTCCAAGAT GGAAGCACAG CTCTTCCAGC TTAAGAATGA ACCGACCACA 
AGAAGCCCAG TTTCCTCTCA ACAATCTTTG AAGAACCTTC GAGAAAGGAG AAACACAGAC 
CTCCCGCTTC TAGACATGCA CACTGTAACC CGGGAAGAGG GAGAAGGCAT GGAGACAACT 
GATACGGAGT CTGTGTCTTC CGCCAGCACA TACACACAGT CTTTAGAGCA GCTGCTTAAC 
TCTCCCGAAA CTAAACTTGA GCCTCCATTA TGGCATGCTG AATTTACCAA AGAAGAATTG 
GTTCAGAAGC TCAGTTCCAC CACAAAAAGT GCAGATCACT TAAACGGCCT GCTTCGGGAA 
ACAGAAGCAA CCAATGCAAT TCTTATGGAG CAAATTAAGC TTCTCAAAAG TGAAATAAGA 
AGATTGGAAA GGAATCAAGA GCGAGAGAAG TCTGCAGCTA ACCTGGAATA CTTGAAGAAC 
GTCTTGCTGC AGTTCATTTT CTTGAAACCA GGTAGTGAAA GAGAGAGACT TCTTCCTGTT 
ATAAATACGA TGTTGCAGCT CAGCCCTGAA GAAAAGGGAA AACTTGCTGC GGTTGCTCAA 
GGTGAGGAAG AAAATGCTTC CCGTTCTTCT GGATGGGCAT CCTATCTTCA TAGTTGGTCT 
GGACTTCGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCC2 ( XM_011512213.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510515.1 Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X3, mRNA.

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CloneID	OHu63067
Clone ID Related Accession (Same CDS sequence)	XM_011512213.2
Accession Version	XM_011512213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	GRIP and coiled-coil domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512213.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG CACATAAGGA GTTGGAACAA TCACATATAA ACTATGTGAA AGAAATTGAA 
AATTTGAAAA ATGAGTTGAT GGCAGTACGT TCCAAATACA GTGAAGACAA AGCTAACTTA 
CAAAAGCAGC TGGAAGAAGC AATGAATACG CAATTAGAAC TTTCAGAACA ACTTAAATTT 
CAGAACAACT CTGAAGATAA TGTTAAAAAA CTACAAGAAG AGATTGAGAA AATTAGGCCA 
GGCTTTGAGG AGCAAATTTT ATATCTGCAA AAGCAATTAG ACGCTACCAC TGATGAAAAG 
AAGGAAACAG TTACTCAACT CCAAAATATC ATTGAGGCTA ATTCTCAGCA TTACCAAAAA 
AATATTAATA GTTTGCAGGA AGAGCTTTTA CAGTTGAAAG CTATACACCA AGAAGAGGTG 
AAAGAGTTGA TGTGCCAGAT TGAAGCATCA GCTAAGGAAC ATGAAGCAGA GATAAATAAG 
TTGAACGAGC TAAAAGAGAA CTTAGTAAAA CAATGTGAGG CAAGTGAAAA GAACATCCAG 
AAGAAATATG AATGTGAGTT AGAAAATTTA AGGAAAGCCA CCTCAAATGC AAACCAAGAC 
AATCAGATAT GTTCTATTCT CTTGCAAGAA AATACATTTG TAGAACAAGT AGTAAATGAA 
AAAGTCAAAC ACTTAGAAGA TACCTTAAAA GAACTTGAAT CTCAACACAG TATCTTAAAA 
GATGAGGTAA CTTATATGAA TAATCTTAAG TTAAAACTTG AAATGGATGC TCAACATATA 
AAGGATGAGT TTTTTCATGA ACGGGAAGAC TTAGAGTTTA AAATTAATGA ATTATTACTA 
GCTAAAGAAG AACAGGGCTG TGTAATTGAA AAATTAAAAT CTGAGCTAGC AGGTTTAAAT 
AAACAGTTTT GCTATACTGT AGAACAGCAT AACAGAGAAG TACAGAGTCT TAAGGAACAA 
CATCAAAAAG AAATATCAGA ACTAAATGAG ACATTTTTGT CAGATTCAGA AAAAGAAAAA 
TTAACATTAA TGTTTGAAAT ACAGGGTCTT AAGGAACAGT GTGAAAACCT ACAGCAAGAA 
AAGCAAGAAG CAATTTTAAA TTATGAGAGT TTACGAGAGA TTATGGAAAT TTTACAAACA 
GAACTGGGGG AATCTGCTGG AAAAATAAGT CAAGAGTTCG AATCAATGAA GCAACAGCAA 
GCATCTGATG TTCATGAACT GCAGCAGAAG CTCAGAACTG CTTTTACTGA AAAAGATGCC 
CTTCTCGAAA CTGTGAATCG CCTCCAGGGA GAAAATGAAA AGTTACTATC TCAACAAGAA 
TTGGTACCAG AACTTGAAAA TACCATAAAG AACCTTCAAG AAAAGAATGG AGTATACTTA 
CTTAGTCTCA GTCAAAGAGA TACCATGTTA AAAGAATTAG AAGGAAAGAT AAATTCTCTT 
ACTGAGGAAA AAGATGATTT TATAAATAAA CTGAAAAATT CCCATGAAGA AATGGATAAT 
TTCCATAAGA AATGTGAAAG GGAAGAAAGA TTGATTCTTG AACTTGGGAA GAAAGTAGAG 
CAAACAATCC AGTACAACAG TGAACTAGAA CAAAAGGTAA ATGAATTAAC AGGAGGACTA 
GAGGAGACTT TAAAAGAAAA GGATCAAAAT GACCAAAAAC TAGAAAAACT TATGGTTCAA 
ATGAAAGTTC TCTCTGAAGA CAAAGAAGTA TTGTCAGCTG AAGTGAAGTC TCTTTATGAG 
GAAAACAATA AACTCAGTTC AGAAAAAAAA CAGTTGAGTA GGGATTTGGA GGTTTTTTTG 
TCTCAAAAAG AAGATGTTAT CCTTAAAGAA CATATTACTC AATTAGAAAA GAAACTTCAG 
TTAATGGTTG AAGAGCAAGA TAATTTAAAT AAACTGCTTG AAAATGAGCA AGTTCAGAAG 
TTATTTGTTA AAACTCAGTT GTATGGTTTT CTTAAAGAAA TGGGATCAGA AGTTTCAGAA 
GACAGTGAAG AGAAAGATGT TGTTAATGTC CTACAGGCAG TCGGTGAATC CTTGGCAAAA 
ATAAATGAGG AAAAATGCAA CCTGGCTTTT CAGCGTGATG AAAAAGTATT AGAGTTAGAA 
AAAGAGATTA AGTGCCTTCA AGAAGAGAGT GTAGTTCAGT GTGAAGAACT TAAGTCTTTA 
TTGAGAGACT ATGAGCAAGA GAAAGTTCTC TTAAGGAAAG AGTTAGAAGA AATACAGTCA 
GAAAAAGAGG CCCTGCAGTC TGATCTTCTA GAAATGAAGA ATGCTAATGA AAAAACAAGG 
CTTGAAAATC AGAATCTTTT AATTCAAGTT GAAGAAGTAT CTCAAACATG TAGCAAAAGT 
GAAATCCATA ATGAAAAAGA AAAATGTTTT ATAAAGGAAC ATGAAAACCT AAAGCCACTA 
CTAGAACAAA AAGAATTACG AGATAGGAGA GCAGAGTTGA TACTATTAAA GGATTCCTTA 
GCAAAATCAC CTTCTGTAAA AAATGATCCT CTGTCTTCAG TAAAAGAGTT GGAAGAAAAA 
ATAGAAAATC TGGAAAAAGA ATGCAAAGAA AAGGAGGAGA AAATAAATAA GATAAAATTA 
GTTGCCGTAA AGGCAAAGAA AGAACTAGAT TCCAGCAGAA AAGAGACCCA GACTGTGAAG 
GAAGAACTTG AATCTCTTCG ATCAGAAAAG GACCAGTTAT CTGCTTCCAT GAGAGATCTC 
ATTCAAGGAG CAGAAAGCTA TAAGAATCTT TTATTAGAAT ATGAAAAGCA GTCAGAGCAA 
CTGGATGTGG AAAAAGAACG TGCTAATAAT TTTGAGCATC GTATTGAAGA CCTTACAAGA 
CAATTAAGAA ATTCGACTTT GCAGTGTGAA ACAATAAATT CTGATAATGA AGATCTCCTG 
GCTCGTATTG AGACATTACA GTCTAATGCC AAATTATTAG AAGTACAGAT TTTAGAAGTC 
CAGAGAGCCA AAGCAATGGT AGACAAAGAA TTAGAAGCTG AAAAACTTCA GAAAGAACAG 
AAGATAAAGG AACATGCCAC TACTGTAAAT GAACTTGAAG AACTTCAGGT ACAACTTCAA 
AAGCAAAAGA AACAGCTTCA GAAAACCATG CAAGAATTAG AGCTGGTTAA AAAGGATGCC 
CAACAAACCA CATTGATGAA TATGGAAATA GCTGATTATG AACGTTTGAT GAAAGAACTA 
AATCAAAAGT TAACTAATAA AAACAACAAG ATAGAAGATT TGGAGCAAGA AATAAAAATT 
CAAAAACAGA AACAAGAAAC CCTACAAGAA GAAATAACTT CATTACAGTC TTCAGTACAA 
CAATATGAAG AAAAAAACAC CAAAATCAAG CAATTGCTTG TGAAAACCAA AAAGGAACTG 
GCAGATTCAA AGCAAGCAGA AACTGATCAC TTAATACTTC AAGCATCTTT AAAAGGTGAG 
CTGGAGGCAA GCCAGCAGCA AGTAGAAGTC TATAAAATAC AGCTGGCTGA AATAACATCA 
GAGAAGCACA AAATCCACGA GCACCTGAAA ACCTCTGCGG AACAGCACCA GCGTACGCTA 
AGTGCATACC AGCAGAGAGT GACAGCACTA CAGGAAGAGT GCCGTGCTGC CAAGGCAGAA 
CAAGCTACTG TAACCTCTGA ATTCGAGAGC TACAAAGTCC GAGTTCATAA TGTTCTAAAA 
CAACAGAAAA ATAAATCTAT GTCTCAGGCT GAAACTGAGG GCGCTAAACA AGAAAGGGAA 
CATCTGGAAA TGCTGATTGA CCAGCTAAAA ATCAAATTAC AAGATAGCCA AAATAACTTA 
CAGATTAATG TATCTGAACT TCAAACATTG CAGTCTGAAC ATGATACACT GCTAGAAAGG 
CACAACAAGA TGCTGCAGGA AACTGTGTCC AAAGAGGCGG AACTCCGGGA AAAATTGTGT 
TCAATACAGT CAGAGAACAT GATGATGAAA TCTGAACATA CACAGACTGT GAGTCAGCTA 
ACATCCCAGA ACGAGGTCCT TCGAAATAGC TTCCGAGATC AAGTGCGACA TTTGCAGGAA 
GAACACAGAA AGACAGTGGA GACATTACAG CAGCAGCTCT CCAAGATGGA AGCACAGCTC 
TTCCAGCTTA AGAATGAACC GACCACAAGA AGCCCAGTTT CCTCTCAACA ATCTTTGAAG 
AACCTTCGAG AAAGGAGAAA CACAGACCTC CCGCTTCTAG ACATGCACAC TGTAACCCGG 
GAAGAGGGAG AAGGCATGGA GACAACTGAT ACGGAGTCTG TGTCTTCCGC CAGCACATAC 
ACACAGTCTT TAGAGCAGCT GCTTAACTCT CCCGAAACTA AACTTGAGCC TCCATTATGG 
CATGCTGAAT TTACCAAAGA AGAATTGGTT CAGAAGCTCA GTTCCACCAC AAAAAGTGCA 
GATCACTTAA ACGGCCTGCT TCGGGAAACA GAAGCAACCA ATGCAATTCT TATGGAGCAA 
ATTAAGCTTC TCAAAAGTGA AATAAGAAGA TTGGAAAGGA ATCAAGAGCG AGAGAAGTCT 
GCAGCTAACC TGGAATACTT GAAGAACGTC TTGCTGCAGT TCATTTTCTT GAAACCAGGT 
AGTGAAAGAG AGAGACTTCT TCCTGTTATA AATACGATGT TGCAGCTCAG CCCTGAAGAA 
AAGGGAAAAC TTGCTGCGGT TGCTCAAGGT GAGGAAGAAA ATGCTTCCCG TTCTTCTGGA 
TGGGCATCCT ATCTTCATAG TTGGTCTGGA CTTCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510515.1
CDS	307..5025
Translation	MGDAHKELEQSHINYVKEIENLKNELMAVRSKYSEDKANLQKQLEEAMNTQLELSEQLKFQNNSEDNVKKLQEEIEKIRPGFEEQILYLQKQLDATTDEKKETVTQLQNIIEANSQHYQKNINSLQEELLQLKAIHQEEVKELMCQIEASAKEHEAEINKLNELKENLVKQCEASEKNIQKKYECELENLRKATSNANQDNQICSILLQENTFVEQVVNEKVKHLEDTLKELESQHSILKDEVTYMNNLKLKLEMDAQHIKDEFFHEREDLEFKINELLLAKEEQGCVIEKLKSELAGLNKQFCYTVEQHNREVQSLKEQHQKEISELNETFLSDSEKEKLTLMFEIQGLKEQCENLQQEKQEAILNYESLREIMEILQTELGESAGKISQEFESMKQQQASDVHELQQKLRTAFTEKDALLETVNRLQGENEKLLSQQELVPELENTIKNLQEKNGVYLLSLSQRDTMLKELEGKINSLTEEKDDFINKLKNSHEEMDNFHKKCEREERLILELGKKVEQTIQYNSELEQKVNELTGGLEETLKEKDQNDQKLEKLMVQMKVLSEDKEVLSAEVKSLYEENNKLSSEKKQLSRDLEVFLSQKEDVILKEHITQLEKKLQLMVEEQDNLNKLLENEQVQKLFVKTQLYGFLKEMGSEVSEDSEEKDVVNVLQAVGESLAKINEEKCNLAFQRDEKVLELEKEIKCLQEESVVQCEELKSLLRDYEQEKVLLRKELEEIQSEKEALQSDLLEMKNANEKTRLENQNLLIQVEEVSQTCSKSEIHNEKEKCFIKEHENLKPLLEQKELRDRRAELILLKDSLAKSPSVKNDPLSSVKELEEKIENLEKECKEKEEKINKIKLVAVKAKKELDSSRKETQTVKEELESLRSEKDQLSASMRDLIQGAESYKNLLLEYEKQSEQLDVEKERANNFEHRIEDLTRQLRNSTLQCETINSDNEDLLARIETLQSNAKLLEVQILEVQRAKAMVDKELEAEKLQKEQKIKEHATTVNELEELQVQLQKQKKQLQKTMQELELVKKDAQQTTLMNMEIADYERLMKELNQKLTNKNNKIEDLEQEIKIQKQKQETLQEEITSLQSSVQQYEEKNTKIKQLLVKTKKELADSKQAETDHLILQASLKGELEASQQQVEVYKIQLAEITSEKHKIHEHLKTSAEQHQRTLSAYQQRVTALQEECRAAKAEQATVTSEFESYKVRVHNVLKQQKNKSMSQAETEGAKQEREHLEMLIDQLKIKLQDSQNNLQINVSELQTLQSEHDTLLERHNKMLQETVSKEAELREKLCSIQSENMMMKSEHTQTVSQLTSQNEVLRNSFRDQVRHLQEEHRKTVETLQQQLSKMEAQLFQLKNEPTTRSPVSSQQSLKNLRERRNTDLPLLDMHTVTREEGEGMETTDTESVSSASTYTQSLEQLLNSPETKLEPPLWHAEFTKEELVQKLSSTTKSADHLNGLLRETEATNAILMEQIKLLKSEIRRLERNQEREKSAANLEYLKNVLLQFIFLKPGSERERLLPVINTMLQLSPEEKGKLAAVAQGEEENASRSSGWASYLHSWSGLR

Target ORF information:

RefSeq Version	XM_011512213.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512213.2

ORF Insert Sequence:

ATGGGAGATG CACATAAGGA GTTGGAACAA TCACATATAA ACTATGTGAA AGAAATTGAA 
AATTTGAAAA ATGAGTTGAT GGCAGTACGT TCCAAATACA GTGAAGACAA AGCTAACTTA 
CAAAAGCAGC TGGAAGAAGC AATGAATACG CAATTAGAAC TTTCAGAACA ACTTAAATTT 
CAGAACAACT CTGAAGATAA TGTTAAAAAA CTACAAGAAG AGATTGAGAA AATTAGGCCA 
GGCTTTGAGG AGCAAATTTT ATATCTGCAA AAGCAATTAG ACGCTACCAC TGATGAAAAG 
AAGGAAACAG TTACTCAACT CCAAAATATC ATTGAGGCTA ATTCTCAGCA TTACCAAAAA 
AATATTAATA GTTTGCAGGA AGAGCTTTTA CAGTTGAAAG CTATACACCA AGAAGAGGTG 
AAAGAGTTGA TGTGCCAGAT TGAAGCATCA GCTAAGGAAC ATGAAGCAGA GATAAATAAG 
TTGAACGAGC TAAAAGAGAA CTTAGTAAAA CAATGTGAGG CAAGTGAAAA GAACATCCAG 
AAGAAATATG AATGTGAGTT AGAAAATTTA AGGAAAGCCA CCTCAAATGC AAACCAAGAC 
AATCAGATAT GTTCTATTCT CTTGCAAGAA AATACATTTG TAGAACAAGT AGTAAATGAA 
AAAGTCAAAC ACTTAGAAGA TACCTTAAAA GAACTTGAAT CTCAACACAG TATCTTAAAA 
GATGAGGTAA CTTATATGAA TAATCTTAAG TTAAAACTTG AAATGGATGC TCAACATATA 
AAGGATGAGT TTTTTCATGA ACGGGAAGAC TTAGAGTTTA AAATTAATGA ATTATTACTA 
GCTAAAGAAG AACAGGGCTG TGTAATTGAA AAATTAAAAT CTGAGCTAGC AGGTTTAAAT 
AAACAGTTTT GCTATACTGT AGAACAGCAT AACAGAGAAG TACAGAGTCT TAAGGAACAA 
CATCAAAAAG AAATATCAGA ACTAAATGAG ACATTTTTGT CAGATTCAGA AAAAGAAAAA 
TTAACATTAA TGTTTGAAAT ACAGGGTCTT AAGGAACAGT GTGAAAACCT ACAGCAAGAA 
AAGCAAGAAG CAATTTTAAA TTATGAGAGT TTACGAGAGA TTATGGAAAT TTTACAAACA 
GAACTGGGGG AATCTGCTGG AAAAATAAGT CAAGAGTTCG AATCAATGAA GCAACAGCAA 
GCATCTGATG TTCATGAACT GCAGCAGAAG CTCAGAACTG CTTTTACTGA AAAAGATGCC 
CTTCTCGAAA CTGTGAATCG CCTCCAGGGA GAAAATGAAA AGTTACTATC TCAACAAGAA 
TTGGTACCAG AACTTGAAAA TACCATAAAG AACCTTCAAG AAAAGAATGG AGTATACTTA 
CTTAGTCTCA GTCAAAGAGA TACCATGTTA AAAGAATTAG AAGGAAAGAT AAATTCTCTT 
ACTGAGGAAA AAGATGATTT TATAAATAAA CTGAAAAATT CCCATGAAGA AATGGATAAT 
TTCCATAAGA AATGTGAAAG GGAAGAAAGA TTGATTCTTG AACTTGGGAA GAAAGTAGAG 
CAAACAATCC AGTACAACAG TGAACTAGAA CAAAAGGTAA ATGAATTAAC AGGAGGACTA 
GAGGAGACTT TAAAAGAAAA GGATCAAAAT GACCAAAAAC TAGAAAAACT TATGGTTCAA 
ATGAAAGTTC TCTCTGAAGA CAAAGAAGTA TTGTCAGCTG AAGTGAAGTC TCTTTATGAG 
GAAAACAATA AACTCAGTTC AGAAAAAAAA CAGTTGAGTA GGGATTTGGA GGTTTTTTTG 
TCTCAAAAAG AAGATGTTAT CCTTAAAGAA CATATTACTC AATTAGAAAA GAAACTTCAG 
TTAATGGTTG AAGAGCAAGA TAATTTAAAT AAACTGCTTG AAAATGAGCA AGTTCAGAAG 
TTATTTGTTA AAACTCAGTT GTATGGTTTT CTTAAAGAAA TGGGATCAGA AGTTTCAGAA 
GACAGTGAAG AGAAAGATGT TGTTAATGTC CTACAGGCAG TCGGTGAATC CTTGGCAAAA 
ATAAATGAGG AAAAATGCAA CCTGGCTTTT CAGCGTGATG AAAAAGTATT AGAGTTAGAA 
AAAGAGATTA AGTGCCTTCA AGAAGAGAGT GTAGTTCAGT GTGAAGAACT TAAGTCTTTA 
TTGAGAGACT ATGAGCAAGA GAAAGTTCTC TTAAGGAAAG AGTTAGAAGA AATACAGTCA 
GAAAAAGAGG CCCTGCAGTC TGATCTTCTA GAAATGAAGA ATGCTAATGA AAAAACAAGG 
CTTGAAAATC AGAATCTTTT AATTCAAGTT GAAGAAGTAT CTCAAACATG TAGCAAAAGT 
GAAATCCATA ATGAAAAAGA AAAATGTTTT ATAAAGGAAC ATGAAAACCT AAAGCCACTA 
CTAGAACAAA AAGAATTACG AGATAGGAGA GCAGAGTTGA TACTATTAAA GGATTCCTTA 
GCAAAATCAC CTTCTGTAAA AAATGATCCT CTGTCTTCAG TAAAAGAGTT GGAAGAAAAA 
ATAGAAAATC TGGAAAAAGA ATGCAAAGAA AAGGAGGAGA AAATAAATAA GATAAAATTA 
GTTGCCGTAA AGGCAAAGAA AGAACTAGAT TCCAGCAGAA AAGAGACCCA GACTGTGAAG 
GAAGAACTTG AATCTCTTCG ATCAGAAAAG GACCAGTTAT CTGCTTCCAT GAGAGATCTC 
ATTCAAGGAG CAGAAAGCTA TAAGAATCTT TTATTAGAAT ATGAAAAGCA GTCAGAGCAA 
CTGGATGTGG AAAAAGAACG TGCTAATAAT TTTGAGCATC GTATTGAAGA CCTTACAAGA 
CAATTAAGAA ATTCGACTTT GCAGTGTGAA ACAATAAATT CTGATAATGA AGATCTCCTG 
GCTCGTATTG AGACATTACA GTCTAATGCC AAATTATTAG AAGTACAGAT TTTAGAAGTC 
CAGAGAGCCA AAGCAATGGT AGACAAAGAA TTAGAAGCTG AAAAACTTCA GAAAGAACAG 
AAGATAAAGG AACATGCCAC TACTGTAAAT GAACTTGAAG AACTTCAGGT ACAACTTCAA 
AAGCAAAAGA AACAGCTTCA GAAAACCATG CAAGAATTAG AGCTGGTTAA AAAGGATGCC 
CAACAAACCA CATTGATGAA TATGGAAATA GCTGATTATG AACGTTTGAT GAAAGAACTA 
AATCAAAAGT TAACTAATAA AAACAACAAG ATAGAAGATT TGGAGCAAGA AATAAAAATT 
CAAAAACAGA AACAAGAAAC CCTACAAGAA GAAATAACTT CATTACAGTC TTCAGTACAA 
CAATATGAAG AAAAAAACAC CAAAATCAAG CAATTGCTTG TGAAAACCAA AAAGGAACTG 
GCAGATTCAA AGCAAGCAGA AACTGATCAC TTAATACTTC AAGCATCTTT AAAAGGTGAG 
CTGGAGGCAA GCCAGCAGCA AGTAGAAGTC TATAAAATAC AGCTGGCTGA AATAACATCA 
GAGAAGCACA AAATCCACGA GCACCTGAAA ACCTCTGCGG AACAGCACCA GCGTACGCTA 
AGTGCATACC AGCAGAGAGT GACAGCACTA CAGGAAGAGT GCCGTGCTGC CAAGGCAGAA 
CAAGCTACTG TAACCTCTGA ATTCGAGAGC TACAAAGTCC GAGTTCATAA TGTTCTAAAA 
CAACAGAAAA ATAAATCTAT GTCTCAGGCT GAAACTGAGG GCGCTAAACA AGAAAGGGAA 
CATCTGGAAA TGCTGATTGA CCAGCTAAAA ATCAAATTAC AAGATAGCCA AAATAACTTA 
CAGATTAATG TATCTGAACT TCAAACATTG CAGTCTGAAC ATGATACACT GCTAGAAAGG 
CACAACAAGA TGCTGCAGGA AACTGTGTCC AAAGAGGCGG AACTCCGGGA AAAATTGTGT 
TCAATACAGT CAGAGAACAT GATGATGAAA TCTGAACATA CACAGACTGT GAGTCAGCTA 
ACATCCCAGA ACGAGGTCCT TCGAAATAGC TTCCGAGATC AAGTGCGACA TTTGCAGGAA 
GAACACAGAA AGACAGTGGA GACATTACAG CAGCAGCTCT CCAAGATGGA AGCACAGCTC 
TTCCAGCTTA AGAATGAACC GACCACAAGA AGCCCAGTTT CCTCTCAACA ATCTTTGAAG 
AACCTTCGAG AAAGGAGAAA CACAGACCTC CCGCTTCTAG ACATGCACAC TGTAACCCGG 
GAAGAGGGAG AAGGCATGGA GACAACTGAT ACGGAGTCTG TGTCTTCCGC CAGCACATAC 
ACACAGTCTT TAGAGCAGCT GCTTAACTCT CCCGAAACTA AACTTGAGCC TCCATTATGG 
CATGCTGAAT TTACCAAAGA AGAATTGGTT CAGAAGCTCA GTTCCACCAC AAAAAGTGCA 
GATCACTTAA ACGGCCTGCT TCGGGAAACA GAAGCAACCA ATGCAATTCT TATGGAGCAA 
ATTAAGCTTC TCAAAAGTGA AATAAGAAGA TTGGAAAGGA ATCAAGAGCG AGAGAAGTCT 
GCAGCTAACC TGGAATACTT GAAGAACGTC TTGCTGCAGT TCATTTTCTT GAAACCAGGT 
AGTGAAAGAG AGAGACTTCT TCCTGTTATA AATACGATGT TGCAGCTCAG CCCTGAAGAA 
AAGGGAAAAC TTGCTGCGGT TGCTCAAGGT GAGGAAGAAA ATGCTTCCCG TTCTTCTGGA 
TGGGCATCCT ATCTTCATAG TTGGTCTGGA CTTCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HIV-1 Nef binds with human GCC185 protein and regulates mannose 6 phosphate receptor recycling.
Biochemical and biophysical research communications474(1)137-145(2016 05)
Kumar M,Kaur S,Nazir A,Tripathi RK

Protein flexibility is required for vesicle tethering at the Golgi.
eLife4(2015 Dec)
Cheung PY,Limouse C,Mabuchi H,Pfeffer SR

ARL4A acts with GCC185 to modulate Golgi complex organization.
Journal of cell science124(Pt 23)4014-26(2011 Dec)
Lin YC,Chiang TC,Liu YT,Tsai YT,Jang LT,Lee FJ

GCC185 plays independent roles in Golgi structure maintenance and AP-1-mediated vesicle tethering.
The Journal of cell biology194(5)779-87(2011 Sep)
Brown FC,Schindelhaim CH,Pfeffer SR

Multiple Rab GTPase binding sites in GCC185 suggest a model for vesicle tethering at the trans-Golgi.
Molecular biology of the cell20(1)209-17(2009 Jan)
Hayes GL,Brown FC,Haas AK,Nottingham RM,Barr FA,Pfeffer SR

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GCC2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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